LOCUS       DBKOIJ010000001        50398 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000001 DBKOIJ010000000
VERSION     DBKOIJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 50398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.001"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            18..899
                     /locus_tag="ACV5NJ_000001"
     CDS             18..899
                     /locus_tag="ACV5NJ_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHY6484135.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            912..2522
                     /locus_tag="ACV5NJ_000002"
     CDS             912..2522
                     /locus_tag="ACV5NJ_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484136.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2599..3258)
                     /locus_tag="ACV5NJ_000003"
     CDS             complement(2599..3258)
                     /locus_tag="ACV5NJ_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HHY6484137.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3541..4206)
                     /locus_tag="ACV5NJ_000004"
     CDS             complement(3541..4206)
                     /locus_tag="ACV5NJ_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HHY6484138.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACV5NJ_000005"
     CDS             complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACV5NJ_000005"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HHY6484139.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACV5NJ_000006"
     CDS             complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACV5NJ_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HHY6484140.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACV5NJ_000007"
     CDS             complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACV5NJ_000007"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HHY6484141.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6520..6840
                     /locus_tag="ACV5NJ_000008"
     CDS             6520..6840
                     /locus_tag="ACV5NJ_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HHY6484142.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6952..7809)
                     /locus_tag="ACV5NJ_000009"
     CDS             complement(6952..7809)
                     /locus_tag="ACV5NJ_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHY6484143.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7917..9221)
                     /locus_tag="ACV5NJ_000010"
     CDS             complement(7917..9221)
                     /locus_tag="ACV5NJ_000010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHY6484144.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9218..9727)
                     /locus_tag="ACV5NJ_000011"
     CDS             complement(9218..9727)
                     /locus_tag="ACV5NJ_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484145.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9902..12037)
                     /locus_tag="ACV5NJ_000012"
     CDS             complement(9902..12037)
                     /locus_tag="ACV5NJ_000012"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HelD family protein"
                     /protein_id="HHY6484146.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12120..13775)
                     /locus_tag="ACV5NJ_000013"
     CDS             complement(12120..13775)
                     /locus_tag="ACV5NJ_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHY6484147.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13777..14091)
                     /locus_tag="ACV5NJ_000014"
     CDS             complement(13777..14091)
                     /locus_tag="ACV5NJ_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HHY6484148.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14261..14521
                     /locus_tag="ACV5NJ_000015"
     CDS             14261..14521
                     /locus_tag="ACV5NJ_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HHY6484149.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14535..14849
                     /locus_tag="ACV5NJ_000016"
     CDS             14535..14849
                     /locus_tag="ACV5NJ_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HHY6484150.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15579..16133)
                     /locus_tag="ACV5NJ_000017"
     CDS             complement(15579..16133)
                     /locus_tag="ACV5NJ_000017"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HHY6484151.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16259..16711)
                     /locus_tag="ACV5NJ_000018"
     CDS             complement(16259..16711)
                     /locus_tag="ACV5NJ_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HHY6484152.1"
                     /translation="MDMSMKLIHQAERYLAEKAYRTQKKEFLPKTAVTNRKENKKERQ
                     LFAKGDRIFVNEYQKEALVYEDIGEDTIDVYLDKKIIHVPRQRVRLVRSAEDLYPTGY
                     DLDSLFIDYKTRKRQRDLERGSKKAHKVLVKEMRKRQEERRVNDENSK"
     gene            complement(16713..18188)
                     /locus_tag="ACV5NJ_000019"
     CDS             complement(16713..18188)
                     /locus_tag="ACV5NJ_000019"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HHY6484153.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGRNRRKPSFVDCS"
     gene            complement(18494..19210)
                     /locus_tag="ACV5NJ_000020"
     CDS             complement(18494..19210)
                     /locus_tag="ACV5NJ_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HHY6484154.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19211..21034)
                     /gene="glpO"
                     /locus_tag="ACV5NJ_000021"
     CDS             complement(19211..21034)
                     /gene="glpO"
                     /locus_tag="ACV5NJ_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HHY6484155.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21048..22544)
                     /gene="glpK"
                     /locus_tag="ACV5NJ_000022"
     CDS             complement(21048..22544)
                     /gene="glpK"
                     /locus_tag="ACV5NJ_000022"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HHY6484156.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22680..24140)
                     /locus_tag="ACV5NJ_000023"
     CDS             complement(22680..24140)
                     /locus_tag="ACV5NJ_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6484157.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24130..25425)
                     /locus_tag="ACV5NJ_000024"
     CDS             complement(24130..25425)
                     /locus_tag="ACV5NJ_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHY6484158.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25757..26434)
                     /locus_tag="ACV5NJ_000025"
     CDS             complement(25757..26434)
                     /locus_tag="ACV5NJ_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HHY6484159.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26464..27555)
                     /locus_tag="ACV5NJ_000026"
     CDS             complement(26464..27555)
                     /locus_tag="ACV5NJ_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HHY6484160.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27656..28090)
                     /locus_tag="ACV5NJ_000027"
     CDS             complement(27656..28090)
                     /locus_tag="ACV5NJ_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHY6484161.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28674..30131)
                     /locus_tag="ACV5NJ_000028"
     CDS             complement(28674..30131)
                     /locus_tag="ACV5NJ_000028"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HHY6484162.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30128..32023)
                     /locus_tag="ACV5NJ_000029"
     CDS             complement(30128..32023)
                     /locus_tag="ACV5NJ_000029"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HHY6484163.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32081..33856)
                     /locus_tag="ACV5NJ_000030"
     CDS             complement(32081..33856)
                     /locus_tag="ACV5NJ_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HHY6484164.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33906..35402)
                     /locus_tag="ACV5NJ_000031"
     CDS             complement(33906..35402)
                     /locus_tag="ACV5NJ_000031"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HHY6484165.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35596..37218)
                     /locus_tag="ACV5NJ_000032"
     CDS             complement(35596..37218)
                     /locus_tag="ACV5NJ_000032"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HHY6484166.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37546..38859)
                     /locus_tag="ACV5NJ_000033"
     CDS             complement(37546..38859)
                     /locus_tag="ACV5NJ_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HHY6484167.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39104..39328)
                     /locus_tag="ACV5NJ_000034"
     CDS             complement(39104..39328)
                     /locus_tag="ACV5NJ_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484168.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39348..39770)
                     /locus_tag="ACV5NJ_000035"
     CDS             complement(39348..39770)
                     /locus_tag="ACV5NJ_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHY6484169.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39912..40622)
                     /locus_tag="ACV5NJ_000036"
     CDS             complement(39912..40622)
                     /locus_tag="ACV5NJ_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B3/B4 domain-containing protein"
                     /protein_id="HHY6484170.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40640..41137)
                     /locus_tag="ACV5NJ_000037"
     CDS             complement(40640..41137)
                     /locus_tag="ACV5NJ_000037"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6484171.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41174..41626)
                     /locus_tag="ACV5NJ_000038"
     CDS             complement(41174..41626)
                     /locus_tag="ACV5NJ_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHY6484172.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41761..42366)
                     /locus_tag="ACV5NJ_000039"
     CDS             complement(41761..42366)
                     /locus_tag="ACV5NJ_000039"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHY6484173.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42816..43436)
                     /locus_tag="ACV5NJ_000040"
     CDS             complement(42816..43436)
                     /locus_tag="ACV5NJ_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHY6484174.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43438..44307)
                     /locus_tag="ACV5NJ_000041"
     CDS             complement(43438..44307)
                     /locus_tag="ACV5NJ_000041"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HHY6484175.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44330..44737)
                     /locus_tag="ACV5NJ_000042"
     CDS             complement(44330..44737)
                     /locus_tag="ACV5NJ_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHY6484176.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44756..46045)
                     /locus_tag="ACV5NJ_000043"
     CDS             complement(44756..46045)
                     /locus_tag="ACV5NJ_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHY6484177.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46103..47442
                     /locus_tag="ACV5NJ_000044"
     CDS             join(46103..46544,46544..47442)
                     /locus_tag="ACV5NJ_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HHY6484178.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            47554..48450
                     /locus_tag="ACV5NJ_000045"
     CDS             47554..48450
                     /locus_tag="ACV5NJ_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HHY6484179.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            48542..48757
                     /locus_tag="ACV5NJ_000046"
     CDS             48542..48757
                     /locus_tag="ACV5NJ_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HHY6484180.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            48775..49047
                     /locus_tag="ACV5NJ_000047"
     CDS             48775..49047
                     /locus_tag="ACV5NJ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HHY6484181.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            49049..49912
                     /locus_tag="ACV5NJ_000048"
     CDS             49049..49912
                     /locus_tag="ACV5NJ_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HHY6484182.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT        14669 a        10487 c         8645 g        16597 t
ORIGIN      
        1 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
       61 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      121 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
      181 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      241 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      301 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      361 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      421 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      481 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      541 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      601 gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg
      661 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      721 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      781 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
      841 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
      901 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
      961 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1021 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1081 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1141 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1201 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1261 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1321 gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag
     1381 cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1441 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1501 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1561 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1621 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1681 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1741 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1801 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     1861 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     1921 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc ttgatttgtc
     1981 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2041 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2101 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2161 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2221 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2281 ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2341 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2401 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2461 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2521 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2581 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2641 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2701 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2761 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
     2821 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     2881 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     2941 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3001 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3061 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3121 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3181 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3241 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3301 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3361 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3421 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3481 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3541 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3601 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3661 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3721 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3781 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     3841 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     3901 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     3961 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4021 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4081 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4141 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4201 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4261 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4321 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4381 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4441 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4501 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4561 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4621 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4681 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4741 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4801 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     4861 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     4921 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     4981 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5041 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5101 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5161 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5221 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5281 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5341 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5401 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5461 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5521 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5581 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5641 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     5701 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5761 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     5821 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     5881 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     5941 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6001 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6061 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6121 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6181 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6241 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6301 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6361 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6421 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     6481 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6541 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6601 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6661 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6721 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6781 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     6841 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     6901 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     6961 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7021 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7081 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7141 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     7201 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     7261 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     7321 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     7381 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     7441 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     7501 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     7561 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     7621 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     7681 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     7741 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     7801 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     7861 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     7921 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     7981 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     8041 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     8101 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     8161 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     8221 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     8281 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     8341 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     8401 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     8461 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     8521 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     8581 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     8641 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     8701 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     8761 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     8821 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     8881 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     8941 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     9001 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     9061 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     9121 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     9181 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     9241 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     9301 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     9361 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     9421 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     9481 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     9541 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     9601 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     9661 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     9721 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     9781 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     9841 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     9901 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     9961 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
    10021 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
    10081 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
    10141 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
    10201 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
    10261 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
    10321 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
    10381 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
    10441 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
    10501 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
    10561 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
    10621 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
    10681 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
    10741 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
    10801 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
    10861 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
    10921 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
    10981 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
    11041 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
    11101 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
    11161 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
    11221 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
    11281 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
    11341 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
    11401 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
    11461 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
    11521 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
    11581 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
    11641 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
    11701 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
    11761 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
    11821 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
    11881 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
    11941 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
    12001 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
    12061 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
    12121 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
    12181 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
    12241 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
    12301 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
    12361 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
    12421 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
    12481 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
    12541 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
    12601 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
    12661 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
    12721 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
    12781 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
    12841 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
    12901 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
    12961 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
    13021 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
    13081 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
    13141 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
    13201 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
    13261 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
    13321 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
    13381 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
    13441 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    13501 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    13561 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    13621 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    13681 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    13741 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    13801 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    13861 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    13921 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    13981 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    14041 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    14101 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    14161 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    14221 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    14281 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    14341 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    14401 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    14461 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    14521 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    14581 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    14641 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    14701 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    14761 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    14821 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    14881 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    14941 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    15001 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    15061 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    15121 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    15181 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    15241 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    15301 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    15361 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    15421 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    15481 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
    15541 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    15601 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    15661 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    15721 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    15781 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    15841 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    15901 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    15961 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    16021 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    16081 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    16141 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    16201 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
    16261 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    16321 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    16381 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    16441 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    16501 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    16561 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
    16621 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    16681 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    16741 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    16801 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    16861 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    16921 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    16981 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    17041 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    17101 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    17161 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    17221 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    17281 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    17341 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    17401 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    17461 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    17521 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    17581 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    17641 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    17701 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    17761 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    17821 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    17881 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    17941 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    18001 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    18061 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    18121 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    18181 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    18241 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    18301 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    18361 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    18421 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    18481 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    18541 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    18601 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    18661 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    18721 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    18781 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    18841 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    18901 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    18961 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    19021 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    19081 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    19141 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
    19201 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    19261 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    19321 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    19381 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    19441 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    19501 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    19561 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    19621 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    19681 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    19741 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    19801 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    19861 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    19921 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    19981 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    20041 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    20101 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    20161 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    20221 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    20281 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    20341 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    20401 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    20461 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    20521 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    20581 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    20641 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    20701 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    20761 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    20821 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    20881 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    20941 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    21001 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    21061 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    21121 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    21181 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    21241 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    21301 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    21361 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    21421 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    21481 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    21541 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    21601 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    21661 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    21721 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    21781 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    21841 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    21901 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    21961 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    22021 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    22081 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    22141 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    22201 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    22261 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    22321 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    22381 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    22441 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    22501 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    22561 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    22621 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    22681 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    22741 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    22801 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    22861 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    22921 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    22981 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    23041 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    23101 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    23161 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    23221 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    23281 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    23341 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    23401 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    23461 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    23521 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    23581 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    23641 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    23701 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    23761 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    23821 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    23881 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    23941 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    24001 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    24061 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    24121 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    24181 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    24241 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    24301 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    24361 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    24421 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    24481 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    24541 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    24601 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    24661 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    24721 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    24781 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    24841 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    24901 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    24961 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    25021 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    25081 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    25141 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    25201 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    25261 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    25321 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    25381 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    25441 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    25501 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    25561 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    25621 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    25681 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    25741 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    25801 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    25861 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    25921 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    25981 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    26041 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    26101 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    26161 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    26221 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    26281 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    26341 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    26401 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    26461 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    26521 atatgctttt tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    26581 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    26641 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    26701 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    26761 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    26821 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    26881 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    26941 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    27001 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    27061 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    27121 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    27181 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    27241 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    27301 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    27361 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    27421 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    27481 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    27541 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    27601 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    27661 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    27721 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    27781 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    27841 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    27901 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    27961 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    28021 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    28081 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    28141 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    28201 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    28261 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    28321 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    28381 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    28441 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    28501 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    28561 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    28621 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    28681 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    28741 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    28801 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    28861 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    28921 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    28981 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    29041 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    29101 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    29161 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    29221 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    29281 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    29341 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    29401 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    29461 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    29521 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    29581 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    29641 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    29701 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    29761 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    29821 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    29881 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    29941 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    30001 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    30061 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    30121 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    30181 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    30241 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    30301 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    30361 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    30421 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    30481 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    30541 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    30601 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    30661 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    30721 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    30781 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    30841 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    30901 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    30961 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    31021 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    31081 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    31141 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    31201 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    31261 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    31321 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    31381 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    31441 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    31501 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    31561 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    31621 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    31681 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    31741 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    31801 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    31861 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    31921 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    31981 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    32041 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    32101 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    32161 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    32221 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    32281 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    32341 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    32401 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    32461 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    32521 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    32581 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    32641 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    32701 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    32761 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    32821 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    32881 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    32941 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    33001 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    33061 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    33121 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    33181 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    33241 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
    33301 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    33361 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    33421 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    33481 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    33541 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    33601 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    33661 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    33721 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    33781 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    33841 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    33901 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    33961 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    34021 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    34081 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    34141 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    34201 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    34261 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    34321 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    34381 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    34441 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    34501 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    34561 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    34621 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    34681 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    34741 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    34801 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    34861 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    34921 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    34981 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    35041 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    35101 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    35161 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    35221 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    35281 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    35341 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    35401 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    35461 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    35521 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    35581 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    35641 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    35701 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    35761 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    35821 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    35881 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    35941 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    36001 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
    36061 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    36121 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    36181 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    36241 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    36301 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    36361 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    36421 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    36481 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    36541 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    36601 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    36661 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    36721 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    36781 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    36841 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    36901 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    36961 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    37021 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    37081 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    37141 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    37201 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    37261 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    37321 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    37381 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    37441 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    37501 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    37561 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    37621 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    37681 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    37741 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    37801 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    37861 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    37921 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    37981 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    38041 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    38101 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    38161 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    38221 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    38281 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    38341 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    38401 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    38461 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    38521 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    38581 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    38641 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    38701 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    38761 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    38821 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    38881 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    38941 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    39001 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    39061 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    39121 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    39181 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    39241 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    39301 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    39361 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    39421 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    39481 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    39541 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    39601 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    39661 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    39721 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    39781 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    39841 tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta
    39901 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    39961 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    40021 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    40081 agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    40141 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    40201 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    40261 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    40321 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    40381 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    40441 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    40501 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    40561 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    40621 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    40681 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    40741 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    40801 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    40861 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    40921 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    40981 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    41041 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    41101 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    41161 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    41221 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    41281 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    41341 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    41401 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    41461 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    41521 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    41581 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    41641 attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    41701 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    41761 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    41821 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    41881 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    41941 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    42001 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    42061 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    42121 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    42181 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    42241 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    42301 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    42361 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    42421 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    42481 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    42541 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    42601 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    42661 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    42721 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    42781 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    42841 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    42901 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    42961 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    43021 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttgc
    43081 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    43141 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    43201 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    43261 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    43321 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    43381 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    43441 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    43501 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    43561 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    43621 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    43681 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    43741 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    43801 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    43861 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    43921 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    43981 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    44041 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    44101 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    44161 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    44221 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    44281 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    44341 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    44401 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    44461 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    44521 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    44581 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    44641 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    44701 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    44761 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    44821 catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact agcaaagcgg
    44881 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    44941 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    45001 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    45061 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    45121 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    45181 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    45241 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    45301 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    45361 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    45421 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    45481 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    45541 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    45601 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    45661 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    45721 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    45781 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    45841 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    45901 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    45961 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    46021 cgatgataaa attgataacg cccatgtttt gatgtgcacc ccaaaagtta gactagaaat
    46081 ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga aaaaagtagt
    46141 ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat atcaaataaa
    46201 tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg aaggcttggt
    46261 tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat atatggaaac
    46321 aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa atagaactct
    46381 aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga gccaaaaaag
    46441 gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta aagatgagtc
    46501 tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa agttagaaac
    46561 cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat ccttcagctc
    46621 aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa gtcaacttat
    46681 tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa gcaagaaatt
    46741 acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac tttagcgtta
    46801 aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc ccaaatggga
    46861 cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt
    46921 gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca
    46981 gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta
    47041 tttaacggag aaataattag ttatagtata tcaactagtc caacatataa actgattgaa
    47101 gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca
    47161 gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga gaataacatt
    47221 attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga aaattttttt
    47281 ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga gatatttcaa
    47341 agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact aaagttaaat
    47401 ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt ttggggttca
    47461 gttcataaag agggctttta tatattaatc acaaatcact tatcacaaat cacaagtgat
    47521 taatcacaaa tcacttgtga tttgtgattc ttaatgatac aatattacta tacaaaaaaa
    47581 gaatggggcg tagttatgga gaaggaaaaa ctaaaaatac ttgaagaatt aagacgtatt
    47641 ttaaacaata aaaatgaagc aattattatc ttgaataatt actttaaagg tggtgttgga
    47701 aagtccaaat tatcgactat gtttgcttac ttgacagaca aattgaattt aaaagtttta
    47761 atgatcgata aggacttaca ggcaacattg acaaaagact tagcaaaaac ttttgaggta
    47821 gaattgccac gtgtcaattt ttatgaaggc ttgaaaaatg gaaacttggc ttcttctatt
    47881 gttcatttga ctgataattt agacttgatc cctggcacgt ttgatttgat gttactgcca
    47941 aaattaactc gctcatggac ttttgaaaat gaaagtagat tgcttgctac tcttttagca
    48001 cctttaaaaa gtgactatga tctcattatt attgatactg taccaacgcc aagcgtttat
    48061 acaaataatg caatcgtggc gagtgattac gttatgatcc ctttacaagc agaagaagaa
    48121 agtacaaaca acattcaaaa ctatatttcc tatttgattg atttacaaga acaatttaac
    48181 cctggactag atatgatcgg ttttgttcct tatttagttg atacggacag cgcaacgata
    48241 aaatcaaacc tggaagaact gtacaagcaa cataaagaag ataacttggt tttccaaaat
    48301 attatcaagc gaagtaataa agtaagtacc tggtctaaaa atggaattac agaacacaaa
    48361 ggctatgaca aaaaagtttt atccatgtat gagaacgtat tttttgaaat gcttgagcga
    48421 attattcaat tagaaaacga aaaagaatag aatcacaaat cacaagtgat taatcacaaa
    48481 tcacttgtga tttgtgattg ttgatgataa aataagaata agaagaaata gaaagaagtg
    48541 agtgattgtg ggaaatttag gcgcacaaaa agcaaaacga aatgatacac caatcagtgc
    48601 aaaaaaagat ataatgggag ataagacggt tcgtgttcgt gctgacttac accatattat
    48661 aaaaattgaa acagcaaaaa atggcggaaa cgtaaaagaa gttatggatc aagccttaga
    48721 agaatatata cggaaatatt tacctgataa actttaaaaa ggagtgaaaa tgaaatggca
    48781 gttacgtatg aaaaaacatt tgaaatagag atcattaacg aattatcggc aagcgtttat
    48841 aatcgagtat taaactatgt tttgaaccat gaactagata ctaaaaatac tcgtttacta
    48901 gaagtgaatc ttttaaatca attagaagtg gcacaagaag ttgatttatt tcaacaacca
    48961 tttgaagaat tacaagctat tcatgagtat tggcggtcaa tgaatcaata ttcaaaacaa
    49021 attttgacaa aagagaaagt ggcttaacat ggcgaatatt actgacttca ccgaaaagca
    49081 atttgaagat cgtttagaaa agaatgttga acgactaact aaaaatagac tagcggttga
    49141 atcgccaacc gcttttttac ttggtgggca accaggatca ggtaaaacta gtttgcgatc
    49201 ggcaatttcc gaagagacac aaggaaatgt tgttatcatt gataatgata cgttcaaaca
    49261 acagcaccct aattttgatg aactagtgaa actttatgaa aaagacgtag taaaatacgt
    49321 taccccttat tctaatcgca tgacagaagc gatcataagc cgtttgagag ataaagggta
    49381 taatttagtg attgaaggta caggacgaac aacagacgtt cctattcaaa ccgcaacaat
    49441 gcttcaagcc aaagattatg aaacaaaaat gtatgtcatg gcagtaccta aaatcaactc
    49501 atatttagga acaattgaac ggtatgaaac tatgtatgca gatgatccaa tgacagccag
    49561 ggcaacacca aaacaagcgc atgatattgt tgtcaaaaac ttaccgacca atttagaaac
    49621 ccttcataaa acgggcttat ttagcgatat aaggctttac aatagagaag gagtgaaact
    49681 ctattcaagc ttagaaacgc cttccattag tccgaaagag accttagaaa gagaattaaa
    49741 tcgtaaagta tcagggaaag aaattcaacc gactttggag agaatagagc aaaaaatggt
    49801 tcaaaatcaa caccaagaaa cccctgaatt taaagcaatt caacaaaaaa tggaaagctt
    49861 gcagccacct acaccaccaa tacccaaaac acctaaactt ccagggattt aaaccttaaa
    49921 aaagtaaaag agtagttacc aaaaaacggt aactactctt tttttataaa aacatttcct
    49981 cattttttaa aagctgatcg agaaataaag cacacaactg tattctttct tttttcagtt
    50041 ggtacatttt gcagtctgta acgtgccatt tactgtttgt taccttaaca tatccagtcg
    50101 aaaacacaag ggtatataaa cgagcaagct cgcccttgtg ttttctttgt gtctatgttg
    50161 cgttctcgtc acagcaaacg acacgaacac actggcgaaa atgaaccaac aaaacgaaaa
    50221 aagaaaggag cgtacactgt aatttatttc tagcttttaa aacatgagga aaacagcttc
    50281 acttcctcaa acgcctaaca aaaatggttg tcaaaaaact aaaaatcaaa aaaagaaaag
    50341 aggaaaagaa aatggaaaaa acaaaaaaat tacaattaga agattttaca gaaaatgg
//
LOCUS       DBKOIJ010000002        47908 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000002 DBKOIJ010000000
VERSION     DBKOIJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.002"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            133..504
                     /locus_tag="ACV5NJ_000049"
     CDS             133..504
                     /locus_tag="ACV5NJ_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HHY6484183.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(778..1470)
                     /locus_tag="ACV5NJ_000050"
     CDS             complement(778..1470)
                     /locus_tag="ACV5NJ_000050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6484184.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1473..2258)
                     /locus_tag="ACV5NJ_000051"
     CDS             complement(1473..2258)
                     /locus_tag="ACV5NJ_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHY6484185.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2495..3895
                     /gene="lacG"
                     /locus_tag="ACV5NJ_000052"
     CDS             2495..3895
                     /gene="lacG"
                     /locus_tag="ACV5NJ_000052"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HHY6484186.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3920..4237
                     /locus_tag="ACV5NJ_000053"
     CDS             3920..4237
                     /locus_tag="ACV5NJ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HHY6484187.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4250..5923
                     /locus_tag="ACV5NJ_000054"
     CDS             4250..5923
                     /locus_tag="ACV5NJ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HHY6484188.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5937..7568
                     /locus_tag="ACV5NJ_000055"
     CDS             5937..7568
                     /locus_tag="ACV5NJ_000055"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 53 protein"
                     /protein_id="HHY6484189.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8059..9624)
                     /gene="guaA"
                     /locus_tag="ACV5NJ_000056"
     CDS             complement(8059..9624)
                     /gene="guaA"
                     /locus_tag="ACV5NJ_000056"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HHY6484190.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9683..10606)
                     /gene="coaA"
                     /locus_tag="ACV5NJ_000057"
     CDS             complement(9683..10606)
                     /gene="coaA"
                     /locus_tag="ACV5NJ_000057"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HHY6484191.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10802..11407
                     /locus_tag="ACV5NJ_000058"
     CDS             10802..11407
                     /locus_tag="ACV5NJ_000058"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHY6484192.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11715..12731
                     /locus_tag="ACV5NJ_000059"
     CDS             11715..12731
                     /locus_tag="ACV5NJ_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6484193.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12872..14173
                     /locus_tag="ACV5NJ_000060"
     CDS             12872..14173
                     /locus_tag="ACV5NJ_000060"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HHY6484194.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14219..14881
                     /gene="deoC"
                     /locus_tag="ACV5NJ_000061"
     CDS             14219..14881
                     /gene="deoC"
                     /locus_tag="ACV5NJ_000061"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HHY6484195.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14902..15300
                     /locus_tag="ACV5NJ_000062"
     CDS             14902..15300
                     /locus_tag="ACV5NJ_000062"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HHY6484196.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15366..16448
                     /locus_tag="ACV5NJ_000063"
     CDS             15366..16448
                     /locus_tag="ACV5NJ_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HHY6484197.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16660..18222
                     /locus_tag="ACV5NJ_000064"
     CDS             16660..18222
                     /locus_tag="ACV5NJ_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484198.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18215..19348
                     /locus_tag="ACV5NJ_000065"
     CDS             18215..19348
                     /locus_tag="ACV5NJ_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484199.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19348..20304
                     /locus_tag="ACV5NJ_000066"
     CDS             19348..20304
                     /locus_tag="ACV5NJ_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484200.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20403..21569
                     /gene="deoB"
                     /locus_tag="ACV5NJ_000067"
     CDS             20403..21569
                     /gene="deoB"
                     /locus_tag="ACV5NJ_000067"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HHY6484201.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21593..22411
                     /locus_tag="ACV5NJ_000068"
     CDS             21593..22411
                     /locus_tag="ACV5NJ_000068"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HHY6484202.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22428..23135
                     /gene="deoD"
                     /locus_tag="ACV5NJ_000069"
     CDS             22428..23135
                     /gene="deoD"
                     /locus_tag="ACV5NJ_000069"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HHY6484203.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23433..24119)
                     /gene="gpmA"
                     /locus_tag="ACV5NJ_000070"
     CDS             complement(23433..24119)
                     /gene="gpmA"
                     /locus_tag="ACV5NJ_000070"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HHY6484204.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24291..24872)
                     /locus_tag="ACV5NJ_000071"
     CDS             complement(24291..24872)
                     /locus_tag="ACV5NJ_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6484205.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25005..25688)
                     /gene="rpiA"
                     /locus_tag="ACV5NJ_000072"
     CDS             complement(25005..25688)
                     /gene="rpiA"
                     /locus_tag="ACV5NJ_000072"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HHY6484206.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26007..26420
                     /gene="rpsL"
                     /locus_tag="ACV5NJ_000073"
     CDS             26007..26420
                     /gene="rpsL"
                     /locus_tag="ACV5NJ_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HHY6484207.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26504..26974
                     /gene="rpsG"
                     /locus_tag="ACV5NJ_000074"
     CDS             26504..26974
                     /gene="rpsG"
                     /locus_tag="ACV5NJ_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HHY6484208.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27068..29155
                     /gene="fusA"
                     /locus_tag="ACV5NJ_000075"
     CDS             27068..29155
                     /gene="fusA"
                     /locus_tag="ACV5NJ_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HHY6484209.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29327..30514
                     /gene="tuf"
                     /locus_tag="ACV5NJ_000076"
     CDS             29327..30514
                     /gene="tuf"
                     /locus_tag="ACV5NJ_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HHY6484210.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30921..31229
                     /gene="rpsJ"
                     /locus_tag="ACV5NJ_000077"
     CDS             30921..31229
                     /gene="rpsJ"
                     /locus_tag="ACV5NJ_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HHY6484211.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31260..31889
                     /gene="rplC"
                     /locus_tag="ACV5NJ_000078"
     CDS             31260..31889
                     /gene="rplC"
                     /locus_tag="ACV5NJ_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HHY6484212.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31917..32540
                     /gene="rplD"
                     /locus_tag="ACV5NJ_000079"
     CDS             31917..32540
                     /gene="rplD"
                     /locus_tag="ACV5NJ_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HHY6484213.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32540..32830
                     /gene="rplW"
                     /locus_tag="ACV5NJ_000080"
     CDS             32540..32830
                     /gene="rplW"
                     /locus_tag="ACV5NJ_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HHY6484214.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32869..33702
                     /gene="rplB"
                     /locus_tag="ACV5NJ_000081"
     CDS             32869..33702
                     /gene="rplB"
                     /locus_tag="ACV5NJ_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HHY6484215.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33745..34023
                     /gene="rpsS"
                     /locus_tag="ACV5NJ_000082"
     CDS             33745..34023
                     /gene="rpsS"
                     /locus_tag="ACV5NJ_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HHY6484216.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34046..34393
                     /gene="rplV"
                     /locus_tag="ACV5NJ_000083"
     CDS             34046..34393
                     /gene="rplV"
                     /locus_tag="ACV5NJ_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HHY6484217.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34407..35063
                     /gene="rpsC"
                     /locus_tag="ACV5NJ_000084"
     CDS             34407..35063
                     /gene="rpsC"
                     /locus_tag="ACV5NJ_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HHY6484218.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35066..35500
                     /gene="rplP"
                     /locus_tag="ACV5NJ_000085"
     CDS             35066..35500
                     /gene="rplP"
                     /locus_tag="ACV5NJ_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HHY6484219.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35490..35678
                     /gene="rpmC"
                     /locus_tag="ACV5NJ_000086"
     CDS             35490..35678
                     /gene="rpmC"
                     /locus_tag="ACV5NJ_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HHY6484220.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35703..35969
                     /gene="rpsQ"
                     /locus_tag="ACV5NJ_000087"
     CDS             35703..35969
                     /gene="rpsQ"
                     /locus_tag="ACV5NJ_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HHY6484221.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36027..36395
                     /gene="rplN"
                     /locus_tag="ACV5NJ_000088"
     CDS             36027..36395
                     /gene="rplN"
                     /locus_tag="ACV5NJ_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HHY6484222.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36432..36740
                     /gene="rplX"
                     /locus_tag="ACV5NJ_000089"
     CDS             36432..36740
                     /gene="rplX"
                     /locus_tag="ACV5NJ_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HHY6484223.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36768..37307
                     /gene="rplE"
                     /locus_tag="ACV5NJ_000090"
     CDS             36768..37307
                     /gene="rplE"
                     /locus_tag="ACV5NJ_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HHY6484224.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37326..37511
                     /locus_tag="ACV5NJ_000091"
     CDS             37326..37511
                     /locus_tag="ACV5NJ_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HHY6484225.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37547..37945
                     /gene="rpsH"
                     /locus_tag="ACV5NJ_000092"
     CDS             37547..37945
                     /gene="rpsH"
                     /locus_tag="ACV5NJ_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HHY6484226.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37977..38513
                     /gene="rplF"
                     /locus_tag="ACV5NJ_000093"
     CDS             37977..38513
                     /gene="rplF"
                     /locus_tag="ACV5NJ_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HHY6484227.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38674..39030
                     /gene="rplR"
                     /locus_tag="ACV5NJ_000094"
     CDS             38674..39030
                     /gene="rplR"
                     /locus_tag="ACV5NJ_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HHY6484228.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39051..39551
                     /gene="rpsE"
                     /locus_tag="ACV5NJ_000095"
     CDS             39051..39551
                     /gene="rpsE"
                     /locus_tag="ACV5NJ_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HHY6484229.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39566..39745
                     /gene="rpmD"
                     /locus_tag="ACV5NJ_000096"
     CDS             39566..39745
                     /gene="rpmD"
                     /locus_tag="ACV5NJ_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HHY6484230.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39789..40229
                     /gene="rplO"
                     /locus_tag="ACV5NJ_000097"
     CDS             39789..40229
                     /gene="rplO"
                     /locus_tag="ACV5NJ_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HHY6484231.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40229..41524
                     /gene="secY"
                     /locus_tag="ACV5NJ_000098"
     CDS             40229..41524
                     /gene="secY"
                     /locus_tag="ACV5NJ_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HHY6484232.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41586..42233
                     /locus_tag="ACV5NJ_000099"
     CDS             41586..42233
                     /locus_tag="ACV5NJ_000099"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HHY6484233.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42424..42642
                     /gene="infA"
                     /locus_tag="ACV5NJ_000100"
     CDS             42424..42642
                     /gene="infA"
                     /locus_tag="ACV5NJ_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HHY6484234.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42675..42791
                     /gene="rpmJ"
                     /locus_tag="ACV5NJ_000101"
     CDS             42675..42791
                     /gene="rpmJ"
                     /locus_tag="ACV5NJ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HHY6484235.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42809..43174
                     /gene="rpsM"
                     /locus_tag="ACV5NJ_000102"
     CDS             42809..43174
                     /gene="rpsM"
                     /locus_tag="ACV5NJ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HHY6484236.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43202..43591
                     /gene="rpsK"
                     /locus_tag="ACV5NJ_000103"
     CDS             43202..43591
                     /gene="rpsK"
                     /locus_tag="ACV5NJ_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HHY6484237.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43671..44609
                     /locus_tag="ACV5NJ_000104"
     CDS             43671..44609
                     /locus_tag="ACV5NJ_000104"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HHY6484238.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44637..45017
                     /gene="rplQ"
                     /locus_tag="ACV5NJ_000105"
     CDS             44637..45017
                     /gene="rplQ"
                     /locus_tag="ACV5NJ_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HHY6484239.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45289..46128
                     /locus_tag="ACV5NJ_000106"
     CDS             45289..46128
                     /locus_tag="ACV5NJ_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HHY6484240.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46104..46973
                     /locus_tag="ACV5NJ_000107"
     CDS             46104..46973
                     /locus_tag="ACV5NJ_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HHY6484241.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46963..47760
                     /locus_tag="ACV5NJ_000108"
     CDS             46963..47760
                     /locus_tag="ACV5NJ_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HHY6484242.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15145 a         8765 c        10509 g        13489 t
ORIGIN      
        1 cagagaacgg gttggttttt aatagattta ataagtactg ataatgaaat ttctcctggt
       61 tgggaaaacg aagttggtta ccctgtagaa gaattttttc aagattactt tgcggagtat
      121 gccgatagga taatgagaga ttacgtaaca gataaaactc aagaagtcat caatcaatcc
      181 cagtcattaa atgaattatc tgataaggcg aaagaagaag cagttgccat tatttcttca
      241 gggattagtg actatattga tatgagtggt gaaatcaatt atggcgataa agacaaaatt
      301 gtacgcatga taaaagcttt tatcaaaact tttgatgtga aggcagatct taagtttaat
      361 gatacttacc tagtaggaaa attaattaat atccttggga cagagaaaga tacggaaacg
      421 tttatttcct ctttgtctat gttgtttaca gataagcatt tttcaactca gcttggtgga
      481 gaggaactag tggaaatctt ttaaccatta cgcccagcat taataaaagt atatcaaaaa
      541 agtactcctg atgattttta agattggttt gagaaataat gtgaaaacac tatttttaat
      601 gttaattata gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa gtcaaagata
      661 cactgctata tttcactaag gatatagcag tgtatctttc atgtcgtttc actggcagtt
      721 ttttataaaa ggaggtgtgc atttcagttg cttttctttg gtagttagtt gaatcgttta
      781 agaattaacg gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat ctttgatggt
      841 tccatcttta tttagctgtg cctgttcaaa tactgttttg tttttaccga atggatcaat
      901 tgcaataatt gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt aagcatcttc
      961 gataacaaga caatcagccg gagctaagct cagtttgtct gccgctttaa gaaaaatatc
     1021 cggttgcggt ttcccaggga aagagccgtc gtcatagaca atcttgtccc agtcaaacca
     1081 gcgggctaaa tcgaaaatat caaaataaaa agcaacattc tctttgacag tagccgtggc
     1141 aatcgttaat ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa gcccttttgt
     1201 caactgaagc tcgttttcat tttgcaagca aagcatacga taatagcttt ctttttcttc
     1261 tgacaatacg gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt tcaaaatctc
     1321 cgtattcgtc cgaccatgaa tattttttaa aatatcctcg tctgataaac catcttttgt
     1381 atgatcatgt atcatatgca gccaagcagc ttcatgcaga tgagagtcca aaaacattgt
     1441 tccgttgaaa tcaaatataa tccctttcat agttacgctc ctatagtggt attttcggtt
     1501 gaatcagcaa tttgcagatc gacatattgt gcatagtgct cttgaacctc ccgcgagatc
     1561 tggcgatccg taatgagcag atcgaaatca tgcaaattat aaaacacata aaaatcatct
     1621 cgattgaatt ttttattatc aacgagtaga tatttcttac gggaattatt tagagcgatc
     1681 tgctgtgctt ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg aacgccattt
     1741 gaactgataa atgcttttgt aaactttagt cttcttaaat tgtcgttggt gatcggtccg
     1801 acaaatgcgc cggtattttt ccggaaatca ccgccaatca gtagaatttc tggtccatct
     1861 gcttgagcaa gaatatcaaa aactggataa ttatttgtaa tgatccgaat tttcttgttc
     1921 agtaaatgcg tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat cgtttctccc
     1981 tcgtgaatca aggaggctgc tgtggcagca atttcttttt tttcttgaat ctgcagagtg
     2041 gctttttcag tgtgagaaag ttcttgatct aaagtgtagg aaagactttg agcccctcca
     2101 tggacacgca gcagcttgcc cgatttttct aattcatcta aatctctgcg aacggtcata
     2161 tcagaaacat ctaattgatt gatgatttca ttaacggtaa ggattccttt tttattgacc
     2221 atttctacga tagtaagcaa tctttctttt ttcaacattg tcttcacctc taatttaagg
     2281 ataatctatc atataaacgg acaacagtca atctaattcg aacaaaagta aacatttagg
     2341 gtggttttta tgtttgttac aatagatttt attgctgttt tctggttttt atgattattt
     2401 gtgtttgttt ttgttgacaa atgttttgga tgcgtttaca atgcaatcag ttagaaagag
     2461 cgaaaactga ttatagattt gaggagagat gtacatgctt aaattaccag aagattttat
     2521 ttttggcgga gccacagcag cctatcaggt tgaaggagcc actaaggaag gtggcaaagg
     2581 cgctgttgct tgggatgact ttttagagga acaagggcga ttcagtcccg atccggcaag
     2641 tgatttttat catcaatatg caaaggacat tgaattgtgt gaacgttttg gtgtaaatgg
     2701 tctaagatta tccattgctt ggagccgcat ttttcctgat ggagctggga aaccaaatcc
     2761 agaaggaatc gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa acgtaacgcc
     2821 gtttgtgacg ttgcatcatt ttgatacccc taaacgtttg tttgaccatg gagatttttt
     2881 gaatcgagaa acaatagaag catttgtttc ctatgccatt ttttgttttc atgaattcaa
     2941 agaagtcaag gtgtggagta cctttaatga aatctatcca gttgcaacca atcaatattt
     3001 gttaggtgtt ttcccaccag gaatcaaata tgattttaca aagatcgtcg cctgtcttca
     3061 taatatgatg gttgctcatg cccgtgtagt gaactatttc aaagaaaacg aacttccggg
     3121 tgaaattggt gtagttcact cattagagac gaaatatgcg gcaacagacg cgccggaaga
     3181 taagcatgct gcctttttag acgatgcatt gtcgatccgt ttcttattag atgctaccta
     3241 tttaggctat tattcaactg aaaccttgac tgctcttgat gagatatgtg aagcaaatca
     3301 ggccagctat cattttcctg aagaagattt cgttgaattg aaaaaagcat ccacgcgtaa
     3361 tgactactta gggatcaatc actaccaatg ccattttgtc aaagcttacg atggagaaaa
     3421 tgcaatccat cataacggta cgggtgaaaa agggacctct gtttataaag tgaagggaat
     3481 cggagaacgt atttataaag aaggaattcc tcgaactgat tgggactggc tgatttatcc
     3541 agaaggtcta tatgacttgt tgctgcgaat taaaagcgat tacccacatt acaacaaaat
     3601 ctatatcact gagaacggga tggggtataa agatcagttt gaagatggca ttattatgga
     3661 tcagccgaga atcgactatt tgagagtcta tttggaatcc ttaagcaagg cgataacagc
     3721 cggggtaaat gtgaaggggt attttttgtg gtcgcttatg gatcttttct catggacaaa
     3781 tgggtataac aaaaggtatg gcttgtttta tgttgatttt gaaacccaga agcgttaccc
     3841 aaaagaatca gcttactggt acaagttggt cagtgaaacc aaaacgatta tttaataagt
     3901 gataatggag gaagaaaaga tgaataaaga ggaattacaa atgcttggtt ttgagatcgt
     3961 tgcatattca ggcgacgctc gcagtacatt attgaaatta ttgaaagaag taagacaagg
     4021 aaactttgaa catgttgatt cggcgttgaa agaagccgac gaaaatttaa cccttgcaca
     4081 taattcgcaa acaaaaattt tagcggaaga agcatctggg aaagagatgg agatgggatt
     4141 catttttatc catgggcagg atcatttaat gaccacttta ttgctgagag atctgattca
     4201 agactttatt gtactgtaca gacagaatca aggataggag aagaacgcta tggatggaat
     4261 cattaaacaa atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta atagttattt
     4321 aggagccgtt cgtgacggat ttttaacagc gatgccagct attttatttt caagcatttt
     4381 cattttgatt gctgcggttc ctgagatttt tggttaccaa tggcctgaat ctgtctccac
     4441 atggctatgg aaagtctata attattcgat gggtgttgta gggttactcg tttcagcaac
     4501 cactgctcgc tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca aagtaatcaa
     4561 caccacctct gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg tctctccgat
     4621 tgaaggtgga ttttcaacgg aatatatggg gacaaaggga ctactggctt cctttgtcgc
     4681 tgcattttta acagttaatt tatataagtt ttgtgtgctg aaagatatta ccattaaaat
     4741 gcctaaagaa gttcctggaa cgatttcgca aaccttccga gatatttttc ctttctcttt
     4801 ttcagtattt gcggccgttt taattgattt aggcattcga caaatcttcc aaatctcatt
     4861 tgcagaagca atcattacgg tgctgcagcc gctatttaca gcagctgacg ggtacttagg
     4921 aatcgctatt atctggggag cgatggcgct gttttggttt gtcggtgttc atggaccgtc
     4981 gatcgtggag ccggcaattg cggcaattat ctatgccaat gtggaaacca atctgcaatt
     5041 gttcaattcc ggtgaacaag cttctaaagt actgacggtg ggtttaggga attttgtagg
     5101 aactatggga ggaactgggg caacattggt tgttcctttt atcttcctac tctttgctaa
     5161 gtctaagcag ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt tcgctgtcaa
     5221 cgaaccattg ctatttgcgg cacccatgat tttgaaccct tattttttta ttccattttt
     5281 gttaacacct gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat tgggcatgaa
     5341 cagctttatg tacgttttgc cttgggcaac tccagcaccg atcggtttgg ttctaggaac
     5401 gggcatgggt atcttagctt ttgttctagc agcggttcta atcgtcgttg acttcctgat
     5461 ctatctacca ttttgcaaag catacgatgc tgtcttagta gagaaagaag cacaaaaagc
     5521 agaggtgatc gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag tacctgtggc
     5581 agcgagtcaa gttatcagcg atgcaaataa agaaatcaat gtgttggtac tttgtgctgg
     5641 cgcagggaca agtgggatgt tagctaatgc tttaaaaaaa ggtgcatctg aatcgaaagc
     5701 accgatcagt gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc aggattttga
     5761 tatggtggtc ttagcaccgc aagtcaattc ttactatgat gatatcaaag aagacaccga
     5821 tcgcttagga atcaagctgg ttgcgacaaa aggagcagaa tatatcggat taacgagaga
     5881 tcctgaaaaa gctgttcagt ttattctgaa ccaatttcaa taaaggagga gccataatga
     5941 aaatgggaat ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg gctttggggg
     6001 ctgtttcaaa aactgttgaa gcatctgcca gcgaatttat tcgcggagca gatatttcga
     6061 tcctgtcaga tatggaaaaa tctggtgcaa aatattatga agacgatatt tcaaaagatg
     6121 ccttagagat tttaaaaaat aatggtgtca actatgttcg actgcgtttg tggcaggatc
     6181 cttataatag taatggagaa agttatggag cagggacaaa tgatctggaa acgacgattg
     6241 accttgcaaa acgagcaaaa aatctgggac ttaaggtgct gctggatttt cactacagtg
     6301 atttttgggt tgatcctggg aaacagaatc ttcctaaagc atggcaaggt ctgacctttg
     6361 aagaaatgaa tacggcattg tacgactata caaagaacgt tctgtcggaa atgaagcagc
     6421 tggacgttta tcctgatatg gttcagatcg gcaatgaact gaatagcgga atgctctggc
     6481 cttatggcaa gtcttgggga gaaggcgggg gagaatttga tcgattggct gcttttttga
     6541 agtctggtat ccaagctgtt cgagacactc aacctaaaac aactccagta atgctccact
     6601 tagctgatgg tggcgataca ggagcattta cttggtggtt tgatgaaatc acgagtcgag
     6661 gtgtgtcatt tgatttaatt ggagtgtcgt attatccgta ttggcacggt tcaatgaatg
     6721 aacttcaaac caatatgaat gcgatcagcc aacggtatgg taaagaagtg atcgtagttg
     6781 aaacagctta tgggcataca acagcgaatg ccgatactat gccaaatgct tttggtgaag
     6841 ccgaagcagc tgcgggggga tatgaaccaa caccgacagg acaagcagag tatttgctgg
     6901 atcttgccga tcggattcag gcagtaccga ataatcgcgg ggcgggcttc ttctattggg
     6961 aacctctttg gtacaacgga aatgtgagtt gggcaacaca agcaggtatg aattatctca
     7021 gtgttcaatc cactatggga aacgaatggg acaaccaagc gatgtttgat tttgctggaa
     7081 acgccttgcc aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat ctagttaaaa
     7141 atgcaagttt cgaaagcgat ggaccaacga ccagtccttc caattggcaa acctggtttc
     7201 aacaaggaac cgctaagggt acgatcaaaa cggaagcggg cacttttttt ggggactaca
     7261 aactaacttt ttggcaggat caagcatacg aggcatcagc ttatcaaaat gttagtggca
     7321 taagcaaagg aacttacaca ttgagtgcct atgtaatgtc tgggggagag tttgatacta
     7381 atcagttata tatcaaaaat tatggtggag cggaactaaa tactgcggtt gttaaaacaa
     7441 gtgaatggac aaaaattaca atcaaagaca ttccaattac aacaggtgtt tgtgaaattg
     7501 gggtttatag caaggcttca ggaaatgctt ggacgaacgt tgattttgtt tcattggtga
     7561 aacaatagac tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc cacatcgtta
     7621 gcgtagaagg caggaatcga acctgcacct cgcctttgcc aaaaataacc agaaacgatt
     7681 tccagtcatt tttaccaaaa cgagtaaagc accagacttc atctacactt tgtattatgc
     7741 taaagagagt tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag ttgtgcagat
     7801 aacataaagt ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata tgatttgagc
     7861 acttagatgg atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa cttcttttta
     7921 tgataagaat gaaacaaatt aacttatatt atagtctctt ttggtaggga accatcttat
     7981 ttttaatgaa aatcaagcat tttagctatt gaagaatgtt gatttatagg gattccccct
     8041 ccatattatg ttggctcttt attcccactc cactgttgct ggtggtttag aagtgatgtc
     8101 atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa
     8161 gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg
     8221 gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc
     8281 tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc
     8341 acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag
     8401 aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat
     8461 gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat
     8521 caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg
     8581 tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga
     8641 gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt
     8701 cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg
     8761 atctcttgca ttgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac
     8821 ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc
     8881 accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc
     8941 aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa
     9001 gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa
     9061 ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg
     9121 tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc
     9181 ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt
     9241 ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg
     9301 gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca
     9361 aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc
     9421 atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac
     9481 ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg
     9541 tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt
     9601 cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa
     9661 atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat
     9721 tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta
     9781 aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat
     9841 ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg
     9901 caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag
     9961 aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga
    10021 tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat
    10081 catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt
    10141 tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga
    10201 ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa
    10261 cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt
    10321 ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat
    10381 gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa agataaatca
    10441 gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac
    10501 ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc
    10561 gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg
    10621 ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga
    10681 gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact
    10741 tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga
    10801 catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc
    10861 ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga
    10921 aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga
    10981 tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt
    11041 tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca
    11101 aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga
    11161 agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa
    11221 aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact
    11281 gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac
    11341 atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa
    11401 agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg
    11461 ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt
    11521 caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga
    11581 acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt
    11641 tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga
    11701 agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag
    11761 taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa
    11821 aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg
    11881 tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct
    11941 tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt
    12001 tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc
    12061 gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag
    12121 acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg
    12181 ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta
    12241 ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag
    12301 aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga
    12361 aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac
    12421 tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct
    12481 atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg
    12541 atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt
    12601 ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa
    12661 aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga
    12721 caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct
    12781 ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat
    12841 aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa
    12901 gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa
    12961 tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat
    13021 gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga
    13081 tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac
    13141 gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc
    13201 tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt
    13261 tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt
    13321 aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt
    13381 cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc
    13441 ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa
    13501 catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg
    13561 agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag
    13621 tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga
    13681 aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga
    13741 tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa
    13801 agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt
    13861 gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt
    13921 ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga
    13981 agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa
    14041 aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt
    14101 attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat
    14161 catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat
    14221 ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt
    14281 gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc
    14341 atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat
    14401 cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat
    14461 caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga
    14521 tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct
    14581 tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga
    14641 aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg
    14701 agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa
    14761 agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac
    14821 aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta
    14881 attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt
    14941 gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta
    15001 acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg
    15061 aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg
    15121 catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa
    15181 gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg
    15241 acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa
    15301 ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta
    15361 cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat
    15421 ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag
    15481 acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc
    15541 agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg
    15601 atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag
    15661 cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc
    15721 aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg
    15781 gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg
    15841 ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg
    15901 aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat
    15961 taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag
    16021 gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag
    16081 gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa
    16141 aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg
    16201 gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag
    16261 atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat
    16321 tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag
    16381 ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag
    16441 aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat
    16501 tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac
    16561 tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat
    16621 cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc
    16681 atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat
    16741 ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct
    16801 acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt
    16861 gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc
    16921 catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc
    16981 gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt
    17041 tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga
    17101 atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt
    17161 gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag
    17221 gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa
    17281 gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt
    17341 aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa
    17401 acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg
    17461 aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa
    17521 gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact
    17581 ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa
    17641 aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac
    17701 gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa
    17761 ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg
    17821 atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc
    17881 ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt
    17941 gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg
    18001 atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt
    18061 ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat
    18121 ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa
    18181 gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg
    18241 taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat
    18301 ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg
    18361 ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt
    18421 aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact
    18481 ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt
    18541 tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc
    18601 aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta
    18661 tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc
    18721 aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac
    18781 acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat
    18841 tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt
    18901 gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat
    18961 ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca
    19021 aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt
    19081 gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa
    19141 gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc
    19201 gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa
    19261 agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa
    19321 tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt
    19381 acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct
    19441 gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt
    19501 tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct
    19561 attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc
    19621 agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta
    19681 ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat
    19741 ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc aattttcttt
    19801 aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc
    19861 atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc
    19921 gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg
    19981 ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt
    20041 acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct
    20101 ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc
    20161 gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg
    20221 ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg
    20281 aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt
    20341 gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa
    20401 tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag
    20461 atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag
    20521 gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg
    20581 tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga
    20641 aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg
    20701 tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta
    20761 aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc
    20821 aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg
    20881 cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata
    20941 taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac
    21001 caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca
    21061 cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg
    21121 atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg
    21181 ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag
    21241 tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg
    21301 aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa
    21361 tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat
    21421 atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat
    21481 attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg
    21541 gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa
    21601 gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat
    21661 tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc
    21721 tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc
    21781 aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt
    21841 ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg
    21901 actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt
    21961 accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat
    22021 cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc
    22081 tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg
    22141 cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg
    22201 gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca
    22261 cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc
    22321 gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc
    22381 attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata
    22441 ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag
    22501 ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta
    22561 tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg
    22621 gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat
    22681 tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac
    22741 tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt
    22801 ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg
    22861 gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga
    22921 cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc
    22981 tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc
    23041 atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga
    23101 tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc
    23161 attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact
    23221 tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt
    23281 aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg
    23341 acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa
    23401 atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt
    23461 aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg
    23521 tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca
    23581 gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc
    23641 aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac
    23701 attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga
    23761 agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt
    23821 tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg
    23881 atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca
    23941 cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt
    24001 ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta
    24061 aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg
    24121 tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta
    24181 caattttttg gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta
    24241 gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat
    24301 aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca
    24361 ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt
    24421 tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct
    24481 tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt
    24541 tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta
    24601 atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat
    24661 ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc
    24721 actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa
    24781 atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt
    24841 atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct
    24901 aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa
    24961 attttccgct gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt
    25021 gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg
    25081 aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc
    25141 tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact
    25201 tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt
    25261 agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg
    25321 actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc
    25381 tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca
    25441 gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct
    25501 aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct
    25561 tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga accggtcccg
    25621 agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt
    25681 aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta
    25741 gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa
    25801 aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc
    25861 tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca
    25921 gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc
    25981 aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc
    26041 gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta
    26101 agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta
    26161 caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa
    26221 acttaattga agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg
    26281 ttgtattatt acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc
    26341 gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa
    26401 ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata
    26461 gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc
    26521 tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt
    26581 gatcaaccgt gtaatggttg acggaaaacg cgggattgca gctaacatta tctataatgc
    26641 ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat
    26701 gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca
    26761 agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa
    26821 ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga
    26881 tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga
    26941 tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta
    27001 taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac
    27061 aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca
    27121 catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca
    27181 taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga
    27241 acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa
    27301 catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt
    27361 acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac
    27421 agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga
    27481 caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa
    27541 cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct
    27601 tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga
    27661 aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt
    27721 agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc
    27781 tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg
    27841 tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt
    27901 gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt
    27961 agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga
    28021 cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc
    28081 atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca
    28141 tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg
    28201 tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga
    28261 atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga
    28321 ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc
    28381 tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga
    28441 tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca
    28501 agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca
    28561 gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg
    28621 tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc
    28681 agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt
    28741 agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac
    28801 tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt
    28861 aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt
    28921 tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg
    28981 ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct
    29041 tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt
    29101 accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct
    29161 tgaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc
    29221 gtgtaaaata attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta
    29281 tcagacaaat aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa
    29341 atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa
    29401 aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat
    29461 ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac
    29521 agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca
    29581 cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt
    29641 agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca
    29701 agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga
    29761 attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg
    29821 tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata
    29881 cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg
    29941 tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg
    30001 tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt
    30061 tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa
    30121 attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg
    30181 tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa
    30241 attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt
    30301 cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt
    30361 accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat
    30421 ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt
    30481 tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa
    30541 catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt
    30601 tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa acacgaaata
    30661 aaagagtcag tattttctaa atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa
    30721 gccttgcttt gtgagtttat atatattata atgacaaagg tgcgtttact ttagtgaatt
    30781 gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg
    30841 acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa
    30901 taggcgaagg agggaacaag atggcaaaac aaaaaattcg tatccgttta aaagcgtatg
    30961 aacaccgtat tttagatcaa tcagcggata aaatcgtgga aactgcaaaa agaactggag
    31021 ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag tctttacaca atcatccgtg
    31081 cgactcataa atacaaagat tcacgcgaac aattcgaaat gcgtacacac aaacgtctta
    31141 tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta gacttaccat
    31201 ctggtgtaaa catcgaaatc aaattataaa actaaaacaa aaataatgga ggtgtactca
    31261 tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca aatcttcact gaatctggtg
    31321 aattaattcc agtaactgtt gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa
    31381 tggaaacgga tggctacgaa gctattcaag ttggttacca agatatgcgt gaagttttat
    31441 caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag cgcttcattc
    31501 gtgaattcaa aaatgttgag ctaggagaat atgaagtagg aaaagaaatt acagtagatg
    31561 ttttccaagc aggagacatt attgatgtaa caggtactac gaaaggtaaa ggattccaag
    31621 gggttatcaa acgccacgga caaagccgcg gaccaatggc tcacggttct cgttaccatc
    31681 gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg
    31741 ctggccgtat gggtggcaac cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg
    31801 cagaaagaaa tgttatcttg atcaaaggta atgttcctgg agctaaaaaa tctttaatta
    31861 ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga aaggaggaac taaggaatgc
    31921 cgaatgtagc attattcaaa caagatggaa gccaaaacgg tgaaatcaca cttaacgaag
    31981 aaatcttcgg aattgaacct aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg
    32041 cgtcattaag acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac
    32101 gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc cgctcaccac
    32161 aatggcgtgg aggtggcgta gtctttggac caacaccacg ttcttacagc tacaaacttc
    32221 ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca
    32281 acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag
    32341 ttcttgctaa cttatctatc gactctaaag tattggttgt tctagaaaaa ggtaacgact
    32401 ttgctgcttt atcagcacgt aacttaccaa acgtttctgt agtaacttct gataatgtga
    32461 gtgtgctaga cgttgtatca aatacaaaag tgttagcaac acaaactgct cttactcaaa
    32521 ttgaggaggt gcttgcataa tgaacttact agacgtaatc aaacgcccag tgatcactga
    32581 aaaatcaatg cttgccatgg atgacaagaa atacactttt gaagtggaca ctcgcgcaaa
    32641 caaaacttta gtaaaacaag cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa
    32701 catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa
    32761 acgtcgcaaa gcgatcgtta cattgactga agattcaaaa gaaatccaat tattcgaagc
    32821 tgctgaataa gcactaatag ataactcatt aaattaggag ggaaaaacgt ggcgattaaa
    32881 aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag gttctgattt tgctgaaatc
    32941 acaacatcaa cacctgaaaa atcattgtta caaccattga aaaaccatgc tggacgtaac
    33001 aacaacggcc gcatcactgt acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg
    33061 atcgacttca aacgtaacaa agataacgtt gttgcaactg ttcaaacgat cgaatatgat
    33121 ccaaaccgtt cagctaacat cgcgttagta cactatgaag atggagtgaa agcttacatc
    33181 ttagcaccta aaggactaga agtgggtatg aaacttgttt caggtccaga agcagatatc
    33241 aaagtcggaa atgctcttcc attagaaaac attccagttg gtactgttat ccacaacatt
    33301 gaaatgaaac ctggtaaagg tggacaattg atccgttctg ctggtacaag tgctcaagta
    33361 cttggtaaag aaggcaaata cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc
    33421 ttagctgcat gccgtgctac aatcggttct gtaggtaacg aacaacacga attaatcaac
    33481 atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc caactgtacg tggtagcgta
    33541 atgaacccta atgatcaccc acacggtggt ggtgaaggta aagctcctat cggacgtaaa
    33601 gcaccagttt ctccttgggg tcaaccagct cttggttaca aaactcgtaa caaaaaagct
    33661 aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat aattactttg accatgtgtc
    33721 gcacattttg agaggaggtt caccatgggt cgtagtttaa agaaaggacc ttttgtcgat
    33781 gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa
    33841 acttggtcac gccgttcaac agttttccca agttttgttg gatataccat cgcagtttat
    33901 gatggacgta aacatgtacc agtttacatc caagaagaca tggtaggaca caaattaggt
    33961 gaatttgcac ctacaagaac ttatcgtgga catgctgctg acgataagaa aacgaaacgc
    34021 taatttgagg ggaggataaa tccaaatggc agaacaaatc acatcagcta aagcaactgc
    34081 aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta attgacctta tcagaggtaa
    34141 aagtgtcgca gatgcaattt caatcttgaa attcacacca aacaaatctg ctggaatcat
    34201 tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat aactttgact tagatgttga
    34261 aaacttggta gtaagcgaag cttttgttaa cgaaggacct acaatgaaac gtttccgtcc
    34321 tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca agtcatatca cggtagtcgt
    34381 atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg
    34441 taggcatcat ccgcgactgg gatgctaaat ggtatgctga aaaagagtat gctgagttct
    34501 tacacgaaga tttaagaatc cgtaaattta tttcaactaa acttgctgat gctgctgtat
    34561 caacagttga aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac acagctaaac
    34621 caggtatggt tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca
    34681 aattaactgg taaaagaatc catatcaata tcgtggaaat caaaaaacca gatctagatg
    34741 caaaattagt aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg
    34801 ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag
    34861 tatctggtcg tctaaacgga gcagatatcg ctcgttcaga aggatactca gaaggaactg
    34921 ttcctcttca tactttacgt gcggacattg attacgcatg ggaagaagca gatacaacat
    34981 acggaaaact aggagttaaa gtgtggatct atcgtggaga aattcttcca actaaaaaaa
    35041 acactgagaa aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga
    35101 attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat tcggtgaata
    35161 cggtttgcaa gctgttgatt cacattggat cacaaaccgc caaatcgaag ctgctcgtat
    35221 cgcaatgact cgttacatga aacgtggtgg gaaagtatgg attaaaattt tccctcacaa
    35281 atcttatact gccaaagcaa ttggggtacg tatgggttct ggtaaagggg cacctgaagg
    35341 atgggttgca ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga
    35401 agtagctcgt gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt
    35461 aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca
    35521 ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga attgtttaat cttagattcc
    35581 aattagcaac aggtcaatta gaaaacactg cacgtattaa agaagtacgt aaatcgattg
    35641 cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt atggaaggag gccattaagc
    35701 gtatgactga aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa
    35761 tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac
    35821 gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa caacacagca aaagttggcg
    35881 acatcgtgag aattatggaa actcgtccat tatcagctac aaaacgtttc cgtttgttgg
    35941 aagtagtcga agaagcagtt attatctaat aacacgagat tttcctatat agattgaatg
    36001 caaaatctga aaggaggata cacaacgtga tccaagcaga aagtcgttta aaagtcgctg
    36061 ataactcagg cgcacgtgaa attttgacga tcaaagtcct tggtggatct ggtcgtaaaa
    36121 ctgctaacat cggtgatgtg atcgttgcta cggttaaaca agcaacgcca ggtggggttg
    36181 tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta
    36241 cagacggttc atatattaaa tttgatgaaa atgctgcagt gattattcgt gatgataaaa
    36301 gccctcgtgg aacacgtatc tttgggccag ttgcacgtga actacgtgaa aacaacttca
    36361 tgaagatcgt ttcactagca ccagaagttt tataatcttg agacacttat caaaggaggt
    36421 gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa agttatcact ggaaaagaca
    36481 aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag
    36541 gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa ggtggaattg
    36601 ttgagatgga agcgccaatt catgtttcta acgtgatggt ggttgactct actggtgtag
    36661 ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg
    36721 gtgaagtttt agataaataa aagtttaagg aaggagggtc ttactgaatg aaccgcctaa
    36781 aagaaaaata tcttaaagaa gtaactccat cattgatgga aaaatttaac tatagctctg
    36841 ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat
    36901 caaacgctaa aaacttagat aaagcagttg aagaattaac tttgatcact ggtcaaaaac
    36961 caatgattac aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa
    37021 ttggtgcaaa agttacattg cgcggagaaa gaatgtacga attcttagac aaattggttt
    37081 ctgtttcact accacgtgta cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc
    37141 gtggaaacta tactttaggt attaaagaac aattgatctt cccagaagta gattatgatt
    37201 tagtagataa agtacgtggt atggacatcg ttattgtaac aacagcaaac tctgatgaag
    37261 aatctcgtga gttgttgaca caattaggca tgccattcca aaaataatta aaggaggcga
    37321 actacgtggc taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac
    37381 aagcttatac tcgttgcgaa cgttgcggac gtccacattc agtttatcgt aagttccatc
    37441 tttgccgtat ttgcttccgc gaacttgcct ataaaggtca aattcccggc gtgaagaaag
    37501 ctagctggta aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg tcatgacaga
    37561 tccaattgca gattttctaa ctcgtatccg taacgcaaac atggttaaac atgagtcttt
    37621 agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt
    37681 cgttcgtgat gttgaataca tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa
    37741 atttggtaaa aacggcgaac gtgttatcac aaacttaaaa cgtatctcta aaccaggttt
    37801 gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg aacggtttag gtatcgcaat
    37861 catttcgact tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg
    37921 cgaagttatc gcttacgtat ggtaatcaag tagaatctac aaggaggtgt ctttaagtga
    37981 gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt aacagttact caagacggaa
    38041 acaacgttac agttaaagga ccaaaaggtg aattgactcg tactttttca gcagatatca
    38101 aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt aaagaaatga
    38161 aaacaattca tggaacaact cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag
    38221 gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa
    38281 aacttgtatt gaacgttggt tactcacatc cagtagaaat cacaccacca gctggagtaa
    38341 ctgttgaagt accagctaac acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg
    38401 gcgaattagc tgcaaacatt cgtggaactc gtcctccaga accttataaa ggtaaaggta
    38461 ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa aactggtaaa taatcgttta
    38521 gaaaaattgc atagaagcca gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa
    38581 aaaatacatt tttacttgtg ccgcagcttc tatttttcga aacgtattta ctgcttgaaa
    38641 tagtagtaca ataaaaataa agaggtgaca attgtgatta caaaaccaga taagaacaaa
    38701 acacgtcaaa agagacatcg tcgtgtacgt aacaatatct ctggtactgc tgagcgccca
    38761 cgcttgaacg tttttcgttc taacaaaaac atctacgctc aaattattga tgacgtagcg
    38821 ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca
    38881 gaagcagcac aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa aggcattaaa
    38941 gtagtagtct ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc tttagctgaa
    39001 gctgctcgcg aaaatggact agaattttag aagaaggagg aataccattc atggtttata
    39061 tcgatccaaa acatttggaa ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag
    39121 ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg
    39181 gacacgtagg atttggtaca ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa
    39241 ttgaagatgc gaagaaaaac ttagttgaag tacctatggt tggttcaaca atcccacacg
    39301 aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa acctgctgta gaaggttctg
    39361 gggttgctgc tggtggacca gttcgtgccg tattagaatt agctggggta gcagatatca
    39421 cttctaaatc tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt
    39481 tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg cggcaaatca gttgaagaat
    39541 taatcggata aggaggacaa caataatggc tgaattaaaa gttactttaa aacgcagtat
    39601 tatcggacgt cctcaaaacc aacgcgatac agttaaagcg ttaggtctta caaagattaa
    39661 cagttcagtg gttaagcctt ctaatgaagc aattaaaggc atgatcaata ctgtttctca
    39721 cctagtggac gttgaagaaa tttaatttag aaaaatcata actagaacat taaggaggtg
    39781 ccaaacctat gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc
    39841 gtgttggtcg tggtacttca tctggtaacg gaaaaactgc cggacgcgga caaaaaggac
    39901 aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga agggggacaa acaccattgt
    39961 tccgtcgttt accaaaacga ggattcacaa acgtgaaccg taaagactat gcagtcatta
    40021 atcttgatgt attgaaccgc tttgaagacg gagctgaagt aactcctgtt gctttagttg
    40081 aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat ggggaactaa
    40141 acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa gcaattgaag
    40201 cggctggtgg atcaatcgag gtgatctaat gtttagacta ttaaaagatg cttttaaagt
    40261 aaaagacatt agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg
    40321 tgcacatatt actgtaccag gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc
    40381 atttttcaac atgttgaact tggtaagtgg tagtgcgatg caaaactttt ccattttctc
    40441 aatgggggtt tcaccataca ttactgcttc tatcgtcatt cagcttcttc aaatggacat
    40501 cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca
    40561 agcgactcga tatctgacat tagtattggc atttatccaa tctgtcggta tcactgctgg
    40621 attcaatcaa tacgctcaat taggtttcgt gaataatcct aacgtagcaa cttacgtaac
    40681 gatcggtttg attctaacag ccggtacgat gtttgttact tggctaggag aacagatcac
    40741 agaaaaaggt atcggaaatg gcgtatcaat gatcattttt gcaggtatca tttctagatt
    40801 gcctgaaagt atcagagaaa tttatgaaga ttactttata aatattgatc cttcagacat
    40861 atggaaatct gctatttttg tggccatttt actagtagcg gtcttgttga tcgtgacatt
    40921 cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc caatatacga aacgcgtagc
    40981 tggcgcgcct acaagcagct atctgccgtt gaaagtaaat gcagctggag ttattccagt
    41041 tatctttgct agttcgttta ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag
    41101 attcggttct gaaaactggt acaaagtgat gatggagatc tttaactata atacagcacc
    41161 tggtgctacg atttatacga ttctgatcgt tgccttcact ttcttctacg cctttgttca
    41221 agtaaaccct gagaaattag cggaaaactt gcaaaagcaa ggaagttata taccaagcgt
    41281 acgccctggt aaggggactg aagagtatgt ctcaagatta ctgatgcgtt taagtacagt
    41341 tggtgcagtc ttccttggtt tagtggcatt gcttccaatc attgctcaaa tggtttggga
    41401 tcttccacaa tcaatcggat taggcggtac aagcttactg atcgttatcg gtgtagcttt
    41461 agaaactgcg aaacagttag aaggcttaat gctgaaacgt aaatatgttg gattcattga
    41521 gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct cacacattta ttaggaggga
    41581 aaacaatgaa cctcatttta atggggctgc caggcgcagg taaaggaaca caagctgaaa
    41641 aaatcattga tgcttatggt attccgcaca tctcaacagg agatatgttc cgcgcagcga
    41701 tgcaaaacga aacagctctt ggcctggaag caaagtctta tatggataaa ggtgcattag
    41761 ttcctgatga agtaacaaat ggaatcgtga aagagcgact agctgaacca gatacagaga
    41821 aaggcttctt attagatggc tttccacgta cgctagatca agcaaaagct ctggacgcaa
    41881 tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa
    41941 tcttggtcga acgtctagcc ggccgattta tctgcagcaa ttgtggagca acttaccata
    42001 aaattttcaa ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg catgaattct
    42061 atcaaagaga agatgataaa cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa
    42121 atagtgaacc aatcttggct tattataaag aacaaggttt gctaaacact attgatggtg
    42181 ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt
    42241 gcccgtagcc gacttgcatt atgcagttac tcttgtcaag ttcaataaat tatgatagaa
    42301 tatgtcagtc cgacttctag agaatcctct agatgatcca ttttgagaat tttgaggtgg
    42361 gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg
    42421 tgcgtggcaa aagaagatat gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat
    42481 gcaatgttta aagttgaact agaaaacgga caccaagttc ttgctactgt ttcaggtaaa
    42541 attcgtatgc attacatccg tatcttacct ggtgacaaag taacggttga attgtctcca
    42601 tacgatctta accgtggccg tattacttat cgctttaaat aattgtactc cgtaaaacta
    42661 caggaggtat tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag
    42721 taattcgtcg taaaggacgc gttatggtga tttgcccagc aaatccaaaa cataaacaac
    42781 gtcaaggata attggaggtg ctataaatat ggctcgtatt gcaggagtag atattcctcg
    42841 tgataaacgt gtagtagttt cacttactta tatctatggt attggtaaca ctactgctaa
    42901 aaagatctta gctgatgctg gtgtatctga agatgttcgc gttcgtgaat taacgaatga
    42961 acaaacagat gctatccgtg cagaagtaga taaattaaaa gttgaagggg atcttcgtcg
    43021 tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca
    43081 tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg
    43141 cccagctcgc gctatcgctg gcaagaaaaa ataattctta agtgaaggag gttaaaactt
    43201 catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat
    43261 tgcacatatc cattctacat tcaataacac tatcatcatg atcactgata ctcacggaaa
    43321 tgcattagcg tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc
    43381 ttttgccgct caaatggcag cagaaactgc tacaaaagca gcaatggaac acggattaaa
    43441 gactgttgaa gtaacagtta aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt
    43501 acaagctaca ggtttagaag tgactgcaat tcgtgacgtg actccagttc ctcataatgg
    43561 tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga gttttatcag
    43621 taacgtcatt gaacaagaca cttttcgttt tgaaaggggt aaagataaga atgattgagt
    43681 tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg
    43741 aacctcttga aagagggtat gggactactt tagggaattc cctacgtcgt attttattat
    43801 cttctttgcc aggtgctgcc atcactaata ttcaaatcga tggtgtactg catgaattct
    43861 ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat cttgaacatt aaaggcttag
    43921 cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat cgatattacc ggacctgcta
    43981 cagtaactgc tggcgatatt atcgttgata gtgatgtaga aattcttaac aaagatatgt
    44041 acatctgtag tgtctctgaa ggagctacgt tccatgctcg cttaacagtg aaacctggac
    44101 gtggttatgt tcaagcagat gaaaataaaa aagaagatat gccaattggc gttcttccag
    44161 tcgattcaat ctacacacct gttcgtcgtg tgaactatca agttgagaac acacgtgttg
    44221 gacaccgtga agattttgat aaattgacga tggagatttg gacagatggt tcaattgaac
    44281 cgttggaagc aatgagtttg tctgcgaaaa tcatgactga acatttggat atttttgtga
    44341 acttaactga tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag
    44401 aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc tgttcgctct tacaattgtt
    44461 tgaaacgtgc aggaatcaat actgtacaag aacttacaaa taaatctgag cctgaaatga
    44521 tcaaagtccg taacttagga cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact
    44581 taggtttagg tttacgtaaa gaagattaaa accttttacg aaggagggaa accaacgtga
    44641 gttatcgtaa actaggacgc acatctagcc aacgtaaagc gatgttgcgt gatttaacaa
    44701 ctgatttatt aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct
    44761 caactgcaga aaaaatgatc actttaggta aacgcggaga tttgcatgca cgtcgtcaag
    44821 ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca
    44881 ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc tcgttacgca gaacgtcaag
    44941 gcggatacac acgcattttg aagacagaac caagacgcgg tgatggtgca ccaatggttg
    45001 tcttagaact tgtctaaaat cacactaata acatcacttt atagatgatt cttttagagt
    45061 gttatgatgt tggaagatat ttttccgagt ctagctcagc gctgctcctc aagcgaatat
    45121 cgcttgggga gtaagcactc atcataaagt gtttttttat accttcaatt acctgtcatg
    45181 acagatatgt ttgaaggcat aaaaaaacat tttaagcgag aaataattga aaattttgtc
    45241 tttttttgat agtgttagta ggaactttag gatgaagaag gagtaatcat ggagccaatt
    45301 attgaattag aaaagataaa ttataaatac cagccagatg atcttcgccc cgcattgaaa
    45361 gatgtctcct ttacaattga taaaggtgag tggatcgcta ttattggaca caatggttca
    45421 ggaaaatcga cacttgccaa aacgatcaat ggattgcttc tgccagaatc agggatcgtg
    45481 aaagtgggaa atcaaatatt ggatgaagaa aatatttgga cgatccgaca gatggttgga
    45541 atggtttttc aaaatccaga caatcagttt gttggttcga cagtagaaga tgacgttgct
    45601 tttggtcttg aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca
    45661 cttgagaaag ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg
    45721 caaaaacaac gtgttgccat tgccggcgtg gttgccttac gacctgatat cattatttta
    45781 gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag aagtgatctc tacgatcaag
    45841 aagatcaaag aagaaagtca attgaccgtt atatctatca ctcatgatat tgatgaagca
    45901 gctaatgcca atcggatact agtgatgaga caaggcgaac ttgtccgtga aggtacacca
    45961 aaagaaattt tttctgctgg tcctgaactg atcgacttag gtctagatct accatttcct
    46021 gaaaaactga agagcgcact gaaagaacgt ggggttgatg tgcccagtga atatatgaca
    46081 gaagaaagga tggtggattg gttatggaca tccgttttga acaagtagac ttcacttatc
    46141 agccgaatac accatttgaa caaagagcct tatttgacat caatatgacg atcaaagaaa
    46201 acagttatac tgcattagta gggcataccg gaagcgggaa atcaacctta cttcagcatt
    46261 tgaatgcgct tgttaagcca acaagcggta cagtccatat tggagaacga gatattcagc
    46321 cggatacgga taataagaat ttaaagccca tccgaaaaaa agttggcatc gtctttcagt
    46381 ttccagaagc acagctattc gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa
    46441 actttggtgt cagcgaagaa gaagcactag tcctagcaaa agaaacatta gaacaagttg
    46501 ggctggatga aagctatttg gaacgttcgc catttgaact ttcaggaggg caaatgcgta
    46561 gagtagcaat cgctggtgtg cttgccatga ggccggaagt acttgtattg gatgaaccaa
    46621 cggcaggact tgatccccag ggcagaaaag aaatgatgga gatgttttgg cgtcttcata
    46681 aggaacagca gatcactatt gtattagtta cgcatttaat ggatgatgta gccaattttg
    46741 cagattatgt ctatgtgttg gaaaagggac gaatcgttaa tagcggggag ccgcaagagg
    46801 tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg cgtacctact gccaccgagt
    46861 ttgcagaaga attaatggct aaagggatga actttgctac cttgcctttg acagctgaag
    46921 aattagcaga cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa taaattgata
    46981 tttggtcgtt atatgcctgg agattccttg atccatcgat tggatccccg agctaaatta
    47041 ttagccagtt tttattttat cggaatcatt tttttagcaa ataattggga aacgtacctt
    47101 ttgttggcat ttttcacatt actttctgtc ttcttgtcaa aagtagattt gaaatttttt
    47161 atccgaggca ttcgcccatt gatctggcta atcctcttca ctgtagcttt gcaggtcttg
    47221 tttacaagtg gcggagaaac ttattggagt tggggtgttt tccatatcac tgaatttggt
    47281 gtgatgaacg gcctctttat tttctgtcga ttcgtgttga tcatttttat gtcgactcta
    47341 ctcacgctta ctacaccgcc tttggaatta tcggatgcga ttgagtactt gcttcgtcca
    47401 ttgaaagccg tacggttccc agtccacgaa atttcattga tgctgtcgat tgctcttcgt
    47461 tttgtgccta cattgatgga cgaaacagaa aagatcatga acgctcagcg cgctcgcggt
    47521 gttgattttg gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc tttgctgatt
    47581 cctttatttg tcagcagctt caaccgtgcg gaagatcttg cgactgctat ggaagcaaga
    47641 ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat tgcattggca taatagagac
    47701 acgatggtta tcattgcttt tgtattatta actgtgctac tgatcttttt aagaggataa
    47761 acgttcagtg ggccgctatc tattcttttc ttgctgatct gtttgcaaga aaggatgcaa
    47821 gcagtgtatg acaagaaata ttagggtctg tggcagacta aggtcacagg cctctttctt
    47881 ttatctgaaa agaaagaacg aaaaaatt
//
LOCUS       DBKOIJ010000003        45811 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000003 DBKOIJ010000000
VERSION     DBKOIJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 45811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.003"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            16..1086
                     /locus_tag="ACV5NJ_000109"
     CDS             16..1086
                     /locus_tag="ACV5NJ_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484243.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1101..1769
                     /locus_tag="ACV5NJ_000110"
     CDS             1101..1769
                     /locus_tag="ACV5NJ_000110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484244.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1789..2367
                     /locus_tag="ACV5NJ_000111"
     CDS             1789..2367
                     /locus_tag="ACV5NJ_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6484245.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2768..3679
                     /locus_tag="ACV5NJ_000112"
     CDS             2768..3679
                     /locus_tag="ACV5NJ_000112"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HHY6484246.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4057..4974)
                     /gene="rnhC"
                     /locus_tag="ACV5NJ_000113"
     CDS             complement(4057..4974)
                     /gene="rnhC"
                     /locus_tag="ACV5NJ_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HHY6484247.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5176..5613
                     /gene="zapA"
                     /locus_tag="ACV5NJ_000114"
     CDS             5176..5613
                     /gene="zapA"
                     /locus_tag="ACV5NJ_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HHY6484248.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5671..6219
                     /locus_tag="ACV5NJ_000115"
     CDS             5671..6219
                     /locus_tag="ACV5NJ_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HHY6484249.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6334..8694
                     /locus_tag="ACV5NJ_000116"
     CDS             6334..8694
                     /locus_tag="ACV5NJ_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HHY6484250.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8781..9095
                     /gene="trxA"
                     /locus_tag="ACV5NJ_000117"
     CDS             8781..9095
                     /gene="trxA"
                     /locus_tag="ACV5NJ_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HHY6484251.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9277..11055
                     /gene="uvrC"
                     /locus_tag="ACV5NJ_000118"
     CDS             9277..11055
                     /gene="uvrC"
                     /locus_tag="ACV5NJ_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HHY6484252.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11200..12099
                     /locus_tag="ACV5NJ_000119"
     CDS             11200..12099
                     /locus_tag="ACV5NJ_000119"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484253.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12092..13354
                     /locus_tag="ACV5NJ_000120"
     CDS             12092..13354
                     /locus_tag="ACV5NJ_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484254.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13515..15413
                     /locus_tag="ACV5NJ_000121"
     CDS             13515..15413
                     /locus_tag="ACV5NJ_000121"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HHY6484255.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15743..16462)
                     /locus_tag="ACV5NJ_000122"
     CDS             complement(15743..16462)
                     /locus_tag="ACV5NJ_000122"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HHY6484256.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16652..16699
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16799..17131
                     /locus_tag="ACV5NJ_000123"
     CDS             16799..17131
                     /locus_tag="ACV5NJ_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484257.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17118..19121
                     /locus_tag="ACV5NJ_000124"
     CDS             17118..19121
                     /locus_tag="ACV5NJ_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HHY6484258.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19140..19610
                     /locus_tag="ACV5NJ_000125"
     CDS             19140..19610
                     /locus_tag="ACV5NJ_000125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HHY6484259.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19631..20215
                     /locus_tag="ACV5NJ_000126"
     CDS             19631..20215
                     /locus_tag="ACV5NJ_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HHY6484260.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20230..21216
                     /locus_tag="ACV5NJ_000127"
     CDS             20230..21216
                     /locus_tag="ACV5NJ_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HHY6484261.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21206..21517
                     /locus_tag="ACV5NJ_000128"
     CDS             21206..21517
                     /locus_tag="ACV5NJ_000128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HHY6484262.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21539..23320
                     /locus_tag="ACV5NJ_000129"
     CDS             21539..23320
                     /locus_tag="ACV5NJ_000129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HHY6484263.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23313..24689
                     /locus_tag="ACV5NJ_000130"
     CDS             23313..24689
                     /locus_tag="ACV5NJ_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HHY6484264.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24696..25331
                     /locus_tag="ACV5NJ_000131"
     CDS             24696..25331
                     /locus_tag="ACV5NJ_000131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HHY6484265.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25361..26716
                     /locus_tag="ACV5NJ_000132"
     CDS             25361..26716
                     /locus_tag="ACV5NJ_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HHY6484266.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMMIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26908..28302)
                     /locus_tag="ACV5NJ_000133"
     CDS             complement(26908..28302)
                     /locus_tag="ACV5NJ_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HHY6484267.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28394..29365
                     /locus_tag="ACV5NJ_000134"
     CDS             28394..29365
                     /locus_tag="ACV5NJ_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HHY6484268.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29362..30144
                     /locus_tag="ACV5NJ_000135"
     CDS             29362..30144
                     /locus_tag="ACV5NJ_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HHY6484269.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30313..30684)
                     /gene="gloA"
                     /locus_tag="ACV5NJ_000136"
                     /gene_synonym="lguL"
     CDS             complement(30313..30684)
                     /gene="gloA"
                     /locus_tag="ACV5NJ_000136"
                     /gene_synonym="lguL"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HHY6484270.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30818..31261
                     /locus_tag="ACV5NJ_000137"
     CDS             30818..31261
                     /locus_tag="ACV5NJ_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HHY6484271.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31304..32113)
                     /gene="yidA"
                     /locus_tag="ACV5NJ_000138"
     CDS             complement(31304..32113)
                     /gene="yidA"
                     /locus_tag="ACV5NJ_000138"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HHY6484272.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32132..33499)
                     /locus_tag="ACV5NJ_000139"
     CDS             complement(32132..33499)
                     /locus_tag="ACV5NJ_000139"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HHY6484273.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33483..34313)
                     /locus_tag="ACV5NJ_000140"
     CDS             complement(33483..34313)
                     /locus_tag="ACV5NJ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HHY6484274.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34476..34913
                     /locus_tag="ACV5NJ_000141"
     CDS             34476..34913
                     /locus_tag="ACV5NJ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HHY6484275.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34989..35609
                     /gene="rpoE"
                     /locus_tag="ACV5NJ_000142"
     CDS             34989..35609
                     /gene="rpoE"
                     /locus_tag="ACV5NJ_000142"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HHY6484276.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35798..36304
                     /locus_tag="ACV5NJ_000143"
     CDS             35798..36304
                     /locus_tag="ACV5NJ_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HHY6484277.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            36321..37220
                     /locus_tag="ACV5NJ_000144"
     CDS             36321..37220
                     /locus_tag="ACV5NJ_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HHY6484278.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            37307..38191
                     /locus_tag="ACV5NJ_000145"
     CDS             37307..38191
                     /locus_tag="ACV5NJ_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HHY6484279.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(38383..38454)
                     /locus_tag="ACV5NJ_000146"
     tRNA            complement(38383..38454)
                     /locus_tag="ACV5NJ_000146"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38419..38421),aa:Arg,seq:ccg)
     gene            38514..39848
                     /gene="rpoN"
                     /locus_tag="ACV5NJ_000147"
     CDS             38514..39848
                     /gene="rpoN"
                     /locus_tag="ACV5NJ_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HHY6484280.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            39997..41088
                     /locus_tag="ACV5NJ_000148"
     CDS             39997..41088
                     /locus_tag="ACV5NJ_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HHY6484281.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            41129..42130
                     /gene="gap"
                     /locus_tag="ACV5NJ_000149"
     CDS             41129..42130
                     /gene="gap"
                     /locus_tag="ACV5NJ_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HHY6484282.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            42240..43430
                     /locus_tag="ACV5NJ_000150"
     CDS             42240..43430
                     /locus_tag="ACV5NJ_000150"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HHY6484283.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            43534..44289
                     /gene="tpiA"
                     /locus_tag="ACV5NJ_000151"
     CDS             43534..44289
                     /gene="tpiA"
                     /locus_tag="ACV5NJ_000151"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HHY6484284.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            44405..45703
                     /gene="eno"
                     /locus_tag="ACV5NJ_000152"
     CDS             44405..45703
                     /gene="eno"
                     /locus_tag="ACV5NJ_000152"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HHY6484285.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        15195 a         7925 c         9776 g        12915 t
ORIGIN      
        1 aggaggagga gttaaatgtt tttagcatta aatgaaatta tgcattcaaa gttacgttat
       61 gcgttagttg caggggtgat gtttttgatc gcctacctgg tatttttttt gactgggtta
      121 gcttatggct tagctcaaga taatcgaaca gcagtcgaca agtgggaggc agattcgatt
      181 gtgctttcga aagatgcaaa ttcaaatcta gggatgtcca tgattactaa gaagatagca
      241 gaagaagtcg aaggtgggaa agtggcgtat cttgcccaaa cacctggagt cgtgactagt
      301 aaagacagta cagaagaagg aaaaatcaat gttagcttct tcggtatcga taaaaatcaa
      361 ttcatcatgc ccaatcttgt tgaaggaaaa gcttttgata acgatgatga ggcagtaggc
      421 gatattagtt tgaaagaaga atatggtcta gccataggag atacagttaa gttatctggt
      481 agtgacaaga cgtttaaact aactggcttt acggatcatg ccaaattcaa tgtctcacca
      541 gttctctata caaccatcaa tgcttaccaa gaaatccgct tcgaaaaaga agatacaagt
      601 gaaaatgcac ggattaacgc aatagttgtc agaggtaaaa tcagtaattt acctgaagat
      661 ttagaacaga tcaaaatttc taaattcatc aatgaattac ctggatataa tgcgcaagta
      721 ttgacttttg gatttatgat cggatttttg atcgttattg cggctattgt gatcgggatt
      781 ttcatttatg tactgacgat gcaaaagatt aatatctttg gtgtaatgaa agcacaaggg
      841 atcacaggag gatttattgc gcgttctgta gtggcacaaa cattcatttt atcatttgtt
      901 ggtattcttc ttggtctatt aggaacagtg ggaacatccc ttgttctacc tgatgcggtt
      961 ccattccaaa gcaattggct gttttttggc gtcattagct tgttgatgtt agtagtagct
     1021 gtgttaggcg cactattttc tgttcggaca atcgtaaaaa tcgatccgtt gaaggcaatt
     1081 ggataagaag gaggaagaac atgaaagcaa tagaatttaa agaagtagaa aaaagtttcc
     1141 aagatggcga ccaagtagtt aaagcattga aagaaacaac catgagtatc gataaagggg
     1201 aatttgttgc aatcatcggt ccttctggtt caggtaaaag tacttttttg actttggcag
     1261 gcggattgca gacgcctagc agtggctctg tatttatcaa tgatcaagaa ttcagtaatc
     1321 aaaaagaaag cgaacgcgtt aaactacgtt ttaaagaaat tggctttatt ttgcaagctt
     1381 ccaatctggt tccttttctg acagtaaaaa agcagttatt attagtagat aagattaaac
     1441 atgaacatcg tcaaaaagaa gcaaacgaat tatttgagca gcttggcgtg gataagttga
     1501 tcaacaagta tccagaagaa ctatctggtg gagaacggca aagggtcgca atcgctcgtg
     1561 cattgtacaa tgatccgtcc attattctag cagatgaacc aactgctagt ttggattcag
     1621 aaaaggcacg agaagtagtc gatatcttgg caaaagaatc aaaagacaaa aataaagcaa
     1681 cgatcatggt tactcacgat acacggctga ttgacaaatg cgatagaata tttatgatgg
     1741 aagatggtgt attgaaagaa aaagaataac caaaaagcag gtggaaaaat ggtccatcga
     1801 tacaacaaag atacacagca aaaaattttg aaagaagcga ctgacttgtt tatgtccaag
     1861 ggatatctcg gcacttcaac aagagaaatc gcacaaaaag ttgggatcac ccagcccaat
     1921 ctctatcatt atttcggtga taaagaaaaa ctatatacgg cagttttaga aaaccactta
     1981 aaagatgttg gaaaagcttt gagagaaatc gttcaaacaa gcgaaacggg atttcaagta
     2041 accttgacaa aaatggcgca gtttttgatt gagacacatc tggtcgattt gtttctgatg
     2101 cttcacgatc ttgaaagcaa tctttcaaag gaaacaagag atcatctttt ctttctatgg
     2161 aagaaaaatt atcgtgaacc ttttgaagaa atcttttcag caaaccaatc tgtgctaaga
     2221 aatgggattt caaaagaaat cgcagcaagg cattttttct tagtgttagc accttacatc
     2281 actcggtcga aaagacctgt ggaagaaccg ctaaatgtgg cgcaattgat cgatttatat
     2341 ttgaatggtg tattaggaga agcgtaaagc tgttatatag ggagaaaata gttaggtgaa
     2401 gaagcaagat atgacgaaga tcgttatatc ttgcttcttt atataggtag tttttcataa
     2461 atacaaattt gttattctct catgattatc agggtatatt tttatttatt atgtcggtgt
     2521 tccaggaagt gttttaattt aataacgaag aaattaattg tttgtagaat gaaggaaact
     2581 atttattggg tagatgaatc accacgaata gtcaaaagga gatttcagga tatgccaaaa
     2641 atgaaaaaaa tcattttagc agtaatgtta ttttctacgg ttatatcttt tacagcccct
     2701 tttttgctta cttaagctga ttttaagttt tggcccttag tctggatcta cgaggtgaga
     2761 gtcaagaatg aaaaaaataa ttggagcttt tgtatttgca atcattatca ttattatctg
     2821 ttttcaaata gatgtttttg gtcaaaaaaa gatagttgga caaacggtca gcagtacgaa
     2881 cagtacggtt acaaagacaa aggaatcatt tgatgataca gaacaagaga cattaatcaa
     2941 aatcggaaaa aatgaccgta cactatatac agatgactcg ctaactaccc cacttgcaac
     3001 tataaacggc ggagagctgg cggagtttct ttctgaaaca aaaaatgctt atcagatcaa
     3061 gacaaatgat ggctatactg gatatttagc cctcgaagat ggaaccaaag tcacaaagaa
     3121 tatacaaaca aaaccggaag agctatctga tgcagtgatt gtattagatc ctgggcatgg
     3181 cggaaatgat actggggcat taagcaatga tgaacaaaca gaagaaaaag atataacatt
     3241 aagtacagcc ataaaagtaa aaaaggcgct agaggatgct ggagcaacgg tttatttgac
     3301 ccatactaca gatgaattag tacagcttgg agatatatgc gactacagtg aagaaaaaaa
     3361 ggcagatgtc tttatcagtt tacacgcaga ctctacggaa tatgcgaatg aagcaactgg
     3421 aatcacgaca tattattact atggacaaga ggaaacatta gcacagacaa ttgctgactc
     3481 cttcactgat ctgcctctgg attcaagagg aatctccact gggaactatc aagttcttcg
     3541 agaaaatctt caaccttcta ttttaatcga gatgggttat atgaataatg atagtgattt
     3601 aaaggaatta gtaacagaca gttatcagca gcagattgct gcaagtttga cacaagcatt
     3661 gaccacttat tttcagtaat aaataagccc agccaaacaa aaatagctcc tcatattttt
     3721 cgtttggccg ggcttattat cgcagatgca agcttttaaa caccgtttaa ggaagaggtc
     3781 gtgagcctgc cgttcaactc tgagtcacaa ggagcaattt caaaaaacag cttctcatgt
     3841 ttgatgacta ttgtgacttg tgaccgagga gttggcatga gaacaccgtt taagaaaaag
     3901 gttgtgaaat caacgttttg ttctgagtat tagagaaaca aacggaaaaa tagttcctca
     3961 tattttgccg tttgttgagc taataccgag gatcagatga ttgaacaccg tttattcgga
     4021 acaagggact gtgacatgac ttttgtcaca gttccattat ttttttagta gcttcaaagc
     4081 tttttctgta ttggcaaaat gaagtttggc atatttttca agcataggca tgccgccttt
     4141 ctccaatagt tttgccgcta cttggtcaga tttcgttcca gctcctgacg gaagggtgat
     4201 tccagcttca gctgatgctt tgtctaactc ttccaaaaag ctggcacgac tgatgataga
     4261 agcagcagca acagctaaat gatattgttc tcctttagta acgaagtaca gtttttcttg
     4321 taattgattt ttttctaaac gcgcatattt tttaaaattt gcttccggcg taaactgatc
     4381 gatcaaaatc gcttctggtt tttctggctc gatttttttt agcagcaaat ggatcgcttg
     4441 attgtgcaaa gcgactttca tccgaacggc attgtatttt ggttggatct cattgtattt
     4501 ttctggagat aagacaagca gtttataggg gatggttttt tttaagatgt cagcaagctg
     4561 gatgatttgg ctatctttta actcttttga atctctaaca ccaagtttac gtaattctgc
     4621 aagctgatca cgtttcacat aagcagcaca tactgttagc ggaccgaaat agctgccatt
     4681 tccgacctca tcactaccta aaacggataa ttgactgata ttttttggca aagaagaggc
     4741 tgtttttgct ggcttggctt gtttatcctg tgcctccccc catttatcag cttctattgc
     4801 tgcattcgta ccttgaaaca tcacttttcc ggatgtataa gcggtaatgg tcgtcccgtc
     4861 tttttttgca gcaaagctag tataaggaat ctgtttattt aacaaataag agcgatagta
     4921 tgctttcatt tgactaagtt ggtcgtttgt tgctttgata atgacgttgc tcataaaatt
     4981 ctcctcgttt ttatacataa aactataccg caactcacga cttttcgttt tctaagagat
     5041 tgcgagacat agcatttttt taaagtttat catataatag ttaagtatgt gaaaaatatt
     5101 tgtggagaac ggtgaaagaa ccaacttcat ggtacactaa ataaaacttt catcaaatag
     5161 gaggaaatga ttcgtatggc gcacgaaaaa acacgctaca aagcggtgat tgccaaccaa
     5221 acatatacta ttatcggacg ggaaaccaag catcatatgg atatcgtaac gaagctgatc
     5281 aatgaacagt tagcagaatt gaaacaatta tcaccacaaa tggataatga acaagcagca
     5341 atcttgatgg ctgtcaatgc gctttcggat caattgaaga aacaagaaag aatattggag
     5401 ctggaagaag aaacagcaga attaaagaaa aaaatgatca aatttacaga gcttgaaaat
     5461 cgtgtgaaac gcattgaagc aatcgagaac gaagctcggg aagtattgaa agaaaatggt
     5521 caagcagatc ataccattaa aaatcatgtg gaagcacaac agattttaaa cgagaaacga
     5581 aaaggacaaa ttaaacaaaa atcttctaat taagatcctt ttatcaaaat aagaaaaatt
     5641 taaaaaagtt agaagagaaa ggaacgtagc atgctgagtt tgctgatttt atttttattg
     5701 ctaattgcgt ttttttctgg agcacgtcgc gggtttgctt tgcaaatcat attcgcgatt
     5761 ggatatgtat tgtcatttat tgctgcccag catttttaca aaccattagc aagtcatttg
     5821 gaattataca ttccataccc agctgtcacc ccaacatcaa agttagcatt ttttgatcag
     5881 gtatttgcct ttcacctaga tgaagctttt tatgcaggaa ctgcttttct gattattttg
     5941 ctaattggct ggctgcttac ccgatttgtg ggggtttttg tccatggatt gacgtatgtg
     6001 ccgatcttga gacaggtaga ctggattgca ggaggcatcc tgtcactaat catggcttac
     6061 gttactatat ttttgatttt gcagttactt gcctttgttc cattagatgt ggtccaaaat
     6121 caattcggcg agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac
     6181 aagatgtatg agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata
     6241 tttcgttttt tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag
     6301 ctcttttttc atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg
     6361 gaatttgaaa aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa
     6421 gaactggcag aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa
     6481 actgaggatg gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaaattgaa
     6541 aatatccgtc cccatatgaa acggattgag atcggagcag acttgaatgg agtggagctt
     6601 gcacaagttt ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt
     6661 tctgacagtg aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc
     6721 cctgttttga gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac
     6781 gcttctcctg agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt
     6841 gaacagttag atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc
     6901 accatgcgaa atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc
     6961 ggtgtagtcc acgatcaaag tgcttcaggt cagactttat ttattgaacc aaaacaagtg
     7021 gtggatttga ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt
     7081 attttaagtg aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatact
     7141 tatgtgattg gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa
     7201 gccatcgtgc cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca
     7261 ctgatcgatc aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc
     7321 atcgtcatta caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg
     7381 ttacaactga tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt
     7441 atttttgagg aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg
     7501 accttttctt ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt
     7561 ctggtattat ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca
     7621 atcgcaatct tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat
     7681 cctgaattga aagtttatgg atataatcgg gcaaatacga ttaatgccag catggagttt
     7741 gatgtggata cattaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat
     7801 gcgtttgaaa tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag
     7861 ttgatgaatg atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg
     7921 aaaatggcag agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt
     7981 cacgatgatt tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa
     8041 aaagcaaaga aaaaagcaaa tgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc
     8101 attgctgata ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag
     8161 ttgatcgatg caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat
     8221 aaagtattga agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt
     8281 acgacttacg gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa
     8341 atcggtatat taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa
     8401 gagccgacac aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat
     8461 caattggacc ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg
     8521 gattctgcca ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc
     8581 gcacttcgca agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag
     8641 tttgcaccgg caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta
     8701 ctaaaggtaa gcagatggtt cgctatccaa gtatagaaat gctatactag tcatagattt
     8761 taaaaatgga ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa
     8821 cagataaagg acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc
     8881 aagcaccgat tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta
     8941 aaatggatgt ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa
     9001 cattaatgtt gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag
     9061 aacaactacg tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag
     9121 ggtgggactg aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa
     9181 ttaatatttt ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct
     9241 ataataactt taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa
     9301 ttagctttac ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc
     9361 atttatgtcg gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggtagc
     9421 cataatacga aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca
     9481 gaatcgaata tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa
     9541 tacaacatta tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa
     9601 tatccccgtt tggtgattac tcgtaaggta ctaaaagaca aagcactgta ttttggacca
     9661 tatcccgatg tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg
     9721 agaaaatgca aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgc
     9781 ctgtgtcctt attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg
     9841 aaaaactttt taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa
     9901 aaagctgctg aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc
     9961 attgagacaa tcatgacttg ccaaaaaatg acaaatactg atttactgga tcgagatgta
    10021 tttggttatg cgattgataa aggatggatg tgtgtccaag tgtttttcgt aagacaagga
    10081 aagctaattg aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc
    10141 ttgacttacc tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg
    10201 attcctgata cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa
    10261 ccaaaacgag gagaaaagaa aaaattagtt gaattggcga gtaagaatgc ctcagttgct
    10321 ttgaatgaac ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa
    10381 cgtttaggaa acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct
    10441 aatatcatgg ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca
    10501 agaaaagaat atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca
    10561 atgagagaag tcatctatcg cagatattcg cgagtgattc gcgaagggtt gcctttacct
    10621 gatttgatct tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa
    10681 aatcaattag gaatcgatat tcctattgct ggcatggtaa aaaacgataa acataaaaca
    10741 aatgagttgc tattcggtac agatttggct gtagtaccgc ttgagcgaaa ttcaccagag
    10801 tttttcttac tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg
    10861 cagctacgca gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca
    10921 aaacgaaaaa aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg
    10981 acagaagaat tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa
    11041 ttacatcagg aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat
    11101 gtacaacaaa aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt
    11161 ctaaggatat atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt
    11221 gttaaaactt ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga
    11281 aaaatcatgg ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc
    11341 ttggattttt tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag
    11401 aaagattatg atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg
    11461 attcaagacc aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa
    11521 ccaaaaatcg acttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta
    11581 aaatctttgt ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa
    11641 cctaaattga tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg
    11701 ctaaaagatg gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac
    11761 aatatggata atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg
    11821 gttttgaatg gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg
    11881 gaatctggtt taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt
    11941 catgaagaca atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt
    12001 actcgtgcga ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa
    12061 gagattttca aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga
    12121 tgtctattta aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat
    12181 catgctgggt atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa
    12241 aatcggcgta ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga
    12301 tgattttgag ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga
    12361 aaaaatcgat gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta
    12421 tagcgaaaat tcattaggac aaagtacaca actactgata caacaacaac tgactggctt
    12481 gcaatcgatg cttcgagcga gtcaattagg catttcacca gaagaagtag ctagcttgag
    12541 ccaacaagca agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt
    12601 cggagaagat aactcgacag tccaatacgt cgtaagttat gtagcaacga ttgtcttgtt
    12661 cattattatt ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac
    12721 gcggatcatg gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaatt
    12781 aacaggtgtt ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat
    12841 aggatacagt caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt
    12901 ggataacttg attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt
    12961 gattttttct gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc
    13021 aaaagcaatt ttgccagtga cttatctatc tctaggcgga tacatgcttg gattgatact
    13081 aggagcttca gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc
    13141 ttcatatatt atgccgattc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat
    13201 ttccttgttg gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta
    13261 caaatcgaat gtactcgttt atagcgaagg cggattatgg agttcattga aacaatcgat
    13321 ttcaattatg aggaatgaac gaaaaaaagc ataaaacaat cctaaaatac agaaggaagc
    13381 tggacatcta ttgttcccag cttctttttg tatagaccta ttttatttat tttactatat
    13441 tggatatata ttcattgaaa atagtgtata acaactttat aatattgagt ataccaattt
    13501 ttggaggcgt tttaatggac cgttatttag atttactttc agaaaaattc ccctcaacgg
    13561 aggatgtcat cacggaaatc atcaatctcg aagcaatttt gcagcttcca aaaggaacag
    13621 agttattttt gagtgatata catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg
    13681 gttcaggaaa tcttaaagaa aagatcaaag accttttttc tgaacaaatg acggaaacgg
    13741 atatcagcca attcactatt tttacggctt atccagaata tgcattaacg actgattggt
    13801 acaaagaaca agataaatcg cagctgatcc atcatttgat cgatctttta cggtttacga
    13861 cagtcaaata tacccggtca aaagttcgaa aaggattgcc gaaagaatat agctatatca
    13921 ttgaggaact cttgtatatc gatgaccgta tcaatggaaa aaaagactat gtaaaaaaaa
    13981 tcattgaaca acttgttttg atgaaggaag aagaaaaatt tttaacgaaa cttgcacaga
    14041 cgatccaaaa atctgtcatc gatcatttgc atatcgttgg agatattttt gaccgaggaa
    14101 ctcaagcagc caaagtgatg gatcgaatca tggatttttc ttctgtagat atccaatggg
    14161 gaaatcatga tattttgtgg attggtgcgt attgcggctc tgaggcatgt ttactgacac
    14221 ttttacggat tgcagcacgc tataattatt tatttgaatt agagaaagaa tatgggttga
    14281 atttacgtcc attgttttct tttgctcatc aaacttatca gaagaatcca gtatttgcgc
    14341 ctaaaaacag taaaaatcta tccgaaagag agcaagaaga attagaaaaa atccatcagg
    14401 cactttctat ccttcaattc aaatcagaac atcagttatt ggatcgtcga cctgaattta
    14461 aaatggacga tcgtaaattg ctggaaaaaa tcgactatga agcatcaacg attgatttag
    14521 aaggtcagct ctatgagttg aaaggaactt gttttcaaac gatccagcca gatgatccaa
    14581 aaaaattctt ggaagaggaa caaaaagtga ttgatagttt gatgtattca ttccagcact
    14641 ctttgagaat gcaaaaacat atgacgtttt tgatcgaaaa agggggaatg tatctcacga
    14701 acaatcagca tgtgctgttt catggctgta tacctgtaga cgagaaggga cagctcttag
    14761 catttgaatt agaccaatct tatcgcggaa aagaactgct gggctttttt gaaaaacaga
    14821 taactgaaag cgcaaaagat ctgactgcaa aagaagattt agcaaccgat ttgatttggt
    14881 atgcgtggag tgggccattt tcgcctttat tcggcaagag taaaatggcg acttttgaac
    14941 gttattttct cacagagcct catacgcacg tcgagaaaag caatccttat tatcacttgc
    15001 gagatgaaga gtggttcagt gaaaaaattt tagcagaatt cgacacaaaa gaagaagggt
    15061 cagcaatcat taatgggcac acgccagtaa aagtaaaaaa aggggagtca ccgatcaaag
    15121 caaatggaaa agtatttgtc attgacggag gtttatccaa agcttatcaa aaaacgacag
    15181 gaattgccgg ttattcacta ttatgcaatt cttatggttt ccaaatcgtg acacattatc
    15241 cgtttttgcc tattgaacaa caatttgcaa aaaaatccga ccaaacaaat gtgaaaaaag
    15301 taatcgaaca gcaactacca cgcaaactca atcgtgatac aacaaaaggt atggaactgc
    15361 agcagcagat tacccagctc aaaaggctgt tggactatcg aaaggacgat tgagaagaat
    15421 tccagtgaac attgttccat agactggaga ctgagacaaa agtcgtgtcg cagtctcttt
    15481 ttccggataa acggtgttct tctgccaact cctcggtcac aagtcacact attcaacaaa
    15541 catgaaaagc tgttttttga aattgctcct tgtgactcag agttgaacgg caggctcacg
    15601 acctcttggt ataaacggtg ttcaaaagcc agtttctcgg tattaagcca aatttatgca
    15661 aaaatgtgaa aagccatttt cacaaaattt ttcttaatac tcgaaactaa acagcttttt
    15721 cacaaccccc tcagaaagtc tattaaaatt cattcacaaa gaaatcgaat aagcgctgtt
    15781 cagatggatg tacacggtgg gtaagttccg gatgccattg aacaccgagg attttatgca
    15841 tatcatcttt gctttgaacc gcttcaacga gtccatcgtt agaaaaagcg atggctttta
    15901 ataaaggtga aagatctttg actgcctgat gatggtaaga attcacaaaa tatggatcgg
    15961 ggagaaggtt gcttaacaaa ctgtctgaaa caatcttgac ttcgtgagtc gtaaattgcg
    16021 gggcagtggg ctgttgttca tgttttactg accactttgg atataatgaa agatcttgat
    16081 aaagtgtacc ttccaaaacg acattcaata gttggagtcc ccggcagact gcaaaaattg
    16141 gttttctttg tttcaatgct tccaagatca gtgcctgttc aaattggtct cgttgaatat
    16201 ttgtttccgt tagctttgga tgaggatctt ccatgtaaag ttgtggactg acatcttgac
    16261 cacctgcaag aataagtttg tcgatttgtc caatcagttg aggagctgaa tctggagaca
    16321 tgactggcag aataagaggc aatccttgag catctaaaac tgcatccacg aatccttgcg
    16381 gtgtatagct cacttgattt ccttggaaag tatctgttgc tcgaatcagt tgatttccgg
    16441 caatgccgat gattggtttc atttgtattc cccctaaagt agacttattg taacaaggat
    16501 ttaaaaaaaa ttaaactatg taagacttgc ccctatactt atctgttctt gctgtgttac
    16561 tatctgtaaa gagaaatttc gtgtgaaatt catgacgata gttgaaaata ttgcttaatt
    16621 tgttataata aaaagtaagc agaaataaaa aggttgggcg taagcttcaa ggatctagtt
    16681 cttcaagggg tgagaaggac attttattga aggggcctgc ctatactaag ttatctttag
    16741 aggtacatac tttcaatgaa aaaagaaagc atgtacctct tttttaaatc gaaaggaagt
    16801 gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa atgaaaagcg
    16861 tgctcaaaca ctgcggcagc agctgaagat ctatgcaaca gaaaaagagc aggaattgga
    16921 aacgatccaa acaaactatc gacaacaact aaaacagttg gtggaagaaa aagaacaatt
    16981 tgaaatacaa gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag acaaggtgca
    17041 agcaactttg aaagatcaat atcaaaagca tcagagtcaa gcgatcggtg cgatcattga
    17101 aagggtgaaa gaaacttatg gccgtcacta agatggaaaa agtgaccctc atttcagata
    17161 aaagaaatca ggagaggatt ttacaagcag tacaaggaat ccaaaacgtt gaaatccggg
    17221 atctgtatca aaatagaaca aacaaccagt gggtggagaa gtattttcca gataccacga
    17281 ttattgataa agaagcaaaa ttaagcgctt taggtaatcg gttgaaagac atccaagaag
    17341 ctatccaatt catacaacac cacggcgata gccgtcaaaa gaaactccat ttgaaacgtc
    17401 aagaactttc cttacaagat ttggaagcca attattccga agaaaatttc ataaataaat
    17461 tgaaagaagt ccttgattta aaagaaaagt gggaacaact aactgaagaa cgtgatcgtt
    17521 tagaagaaga ggaagaatgg cttcttcatt ggcaacagct ggatattgct ccaagtatgt
    17581 atgcaagtaa gacgaccatt tttgagatgg ggactgtgag tgcagcaaat tttgaaagtt
    17641 tcaaggaaga actgagtaag cttgatgacg tttatatgga agaaatagat tatacgccaa
    17701 aacaagttta tctttcgtat gttgctttaa aaaaatcagt tgctgttata gaagaaattg
    17761 ctgggaaata cgggttcaat aaagaaacgt atccttatga acaaaatcct aaagaaaaac
    17821 tgcaagagac caagcagcaa ttatccattg ttcacgagca gcaaaagaag ataacgacag
    17881 cgttaggtca gtgcagtggc tatatccaag aatttgaatg ggtagaagaa gtcacacttg
    17941 ccgttgcaga acgagaaaaa ataaaagaac ggtttatcca tgcgacttat ctgatcgttt
    18001 tgcaaggttg ggtagatgca gaagaaaaac agaatttgct gcatgtgtta aacgaaaccg
    18061 tatcagaaga agatatatac gtcagtttcg aaacaccaaa ctcagaagag attcagcagg
    18121 aagtccctac taaattgaaa aaccatccgc ttgtcgaacc ttttgaattg ctgactgaaa
    18181 tgtacagtct gccaaaatat gaagaagtag atccaacacc ttggatgaca ccattttatc
    18241 ttgtcttctt cgggatgatg gtcgcagatg ttggttatgg tttgctgatg ctcattggga
    18301 caatactagc tcaaaaacta cttgttctac cacgcgggat gaaacgtttc gtaaaattct
    18361 ttgagattct ctcgatacca tcgatcatct ggggattgat ctacagttca tttttcggac
    18421 agtcattacc aaaagaactg tttggcatcc gcttgccttt cccaatccta tctacgacgg
    18481 atgacgtcaa tacgattttg attttatccg tgattttcgg tctgatacag attttggttg
    18541 gattatttgt ggcagcaaag gaaaatatca ggagaaaaga ttacttgagt gctgtcagtg
    18601 atggatttgc ctggcaaggg atcctaattg ggatcgttat tgcgttggtt ggtgccatgt
    18661 tgataaaaaa tacagcattt gtttaccttg gcgctgggct atcgatcatt gctgccttat
    18721 gtatcttgat cgttccgatt atccaaacgc cgtctaaagt caaaggtgct gccaaaggtg
    18781 cgtataatct ttacggactg accggctata tcggtgattt agtcagttat acacgtttga
    18841 tggcattggg tatttcaggc ggaagtattg gagcggcatt caatatgcta gttgctttca
    18901 tgccgccagc tgctcgattc agtgtaggga tccttttgat catcgcttta cacgcactga
    18961 atatgttttt gacactgcta agtgcctatg tccatggcgc acgattgcag tatgtcgaat
    19021 tttttgggaa gttttataca ggaggcggac gtgcctttga tccgctgaaa acggcggaaa
    19081 aatacgtcaa catcaatcac aagaaaaaaa acaataaata attggaggat ttttatataa
    19141 tgatggatta cttaattagt caaaatggtg gaatggtatt tgcggtatta gcgatggcaa
    19201 cagcgacgat tttttcaggg atcggttcag ctaaaggtgt tggaatgaca ggtgaggcag
    19261 cagcagcatt gacaacaagt caacccgaaa aattcgggca ggctctgatt ctgcaattgc
    19321 ttccaggcac gcaaggtttg tatggattcg ttattgcctt tttgattttt atcaacttag
    19381 gaaatgacat gtcagttgta caaggactga actttctagg tgcttctctg ccgattgctt
    19441 ttacaggctt gttttcaggt attgcccaag gaaaagtcgc agcggcaggt attcaaattt
    19501 tagcgaaaaa acctgaacat gctacaaaag gaatcatttt tgctgcgatg gttgaaacat
    19561 acgcgatttt aggtttcgtt atttccttcc tattagtgtt gaatgcctaa gcataaagga
    19621 ggaaaagcag atgaacgcca ttgaaaaaat catctctcag atgaacgaag cagcagagca
    19681 ggaaagagcg gcgttagaac aagaagagcg aatgaagatc gatcaaaact ttgaacaaaa
    19741 acgtacgcaa gttgaaacag aacatcaaaa acaaaaagaa aaacaaatcg aattactcga
    19801 aaaaaaatac cgtcagctaa ggaatcgcca gcaagtagaa gtacgacaag aaaatttgaa
    19861 tgcgaagcag gaatttttaa gacgattgtt tgcagatgcg gtaactgaaa tggaaaattg
    19921 ggatgaatct gaacaaatcc agtttatcaa aaatgcattg tattcattac ctttgacagg
    19981 aaaagtagca ttcattgctg gagaaaaatc agcagcctat ctttctcaga cgttgttgga
    20041 tgaatggaac aatgaacttc cctttatgat ggttttaagt gatgaaactg ttgctgatca
    20101 agcgggtttt ctgataaacg atcaaggagt tcagtataat tttcttttca gcagccttgt
    20161 acaagatatc caaggaacga tgagttttga gattgccaat caattattcg aataaggagg
    20221 gatcagtgta tggaatacca cgaactaaat cccttgattc gcggaagaga gcttgaactt
    20281 ttgtccaatg agacatttga acggatgatt caaacaaatt ctgtcgaagc acttggtgaa
    20341 atattgaaat ccacgattta cagtccatat atttatgacg gatatgaata tgacttcgaa
    20401 cagaatcttt taaaggagca gagccgtcta tttcgctggt taaaggaatc agcaccggag
    20461 cctgaaatcg tttggatcta tacgatgcgt tatactttcc ataatctgaa ggttttgaca
    20521 aaagctgaga tgacaaatca aaatctggac catttgtata tcgacgatgg attttattca
    20581 ttagaaacct tgaaggatgc cattcatacg caagtttcaa atgaacttcc tgaaagcatt
    20641 atggacagta tacgagaagt acgtgattat tttgaagagt ctaccattct tcagggaatt
    20701 gacgtgattt atgatcgaca gtttttgaca gagcagcgac ggcttgccga taagctagga
    20761 tatcaagaat tgctgacaga agtcattgct tttattgact tgaccaatat cacaacgatg
    20821 gctcgaggaa tattgcagaa aagaacatat gggttcatga cgacggtact ttccagctcg
    20881 ggaagtatcg aaaaagaagt atttttgaac tttgtagata aagatatgga agattacacg
    20941 aattttctat tagagacaga ttatgctgat tttctgcgcc ctgtcatccg tgagggaaat
    21001 attgatctcg taaagcttga acagttgaaa gatgattatc taagttcatt gtatcaagaa
    21061 gcacaaaccc aggctttcgg accactgcca ctactagcct ttttgaacgc aaaagaggta
    21121 gaaagcaaaa accttcgttt actggttatc ggcaaaagaa gccatttcga atcagaaaca
    21181 atcagaaagc gggtgagaca agtctatgac gcataagatc ggtgtagttg gcgacaaaga
    21241 ttctgttttg ccattcaaat tgtttggctt tgatgttcgt tatggtacaa ccaagcaaac
    21301 cgttcgtcag gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt atgtcacaga
    21361 gcaatgtgca gcattgagcc cagaaacgat cgaacgttac aaggaacaaa tgatacctgc
    21421 tatcgtattg attcccaatc atcaaggatc attagggata gggctggaag aaattcaaaa
    21481 aaacgtagaa aaagcagtag gtcaaaatat cttatagtat gaaaaggaga tagaaatttt
    21541 gcaaattgga aaaatcataa aagtctccgg acctcttgta atggctgaga acatgtctga
    21601 tgccagtatc caagacatgt gtctagtggg agatctagga gttatcggag aaattatcga
    21661 gatgcgtggc gatgtggcgt ctattcaagt atatgaagag acttctggaa ttggtccagg
    21721 cgaaccggtt cgctctactg gtgaagcttt atctgttgaa ttaggaccag ggatcatttc
    21781 acaaatgttt gacggaatcc aacggccatt agataccttt atggaaatca cacaaagtaa
    21841 tttcttaggg cgcggagttc aattgcccgc attggaccat gagaaaaaat ggtggttcga
    21901 accaacagta gaagccggag aaacggtgag tgctggagat gttatcggga tcgtagaaga
    21961 aacaaaagtt ataaaacata aaatcatggt gccaaatggg ataaaaggaa cgatcaaaac
    22021 aatcgaaaaa ggttcattca ctattgatga aacagtatgt gtcattgaaa ccgaaaatgg
    22081 tgacaaaaat ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc caatcaaaga
    22141 aaaattaaac ccggatgcac ccatgatcac tggacagcga gtgattgata cattcttccc
    22201 agtgacaaaa ggaggagcag cagctgtacc tggtcctttt ggtgcaggga aaacagtcgt
    22261 tcagcatcaa attgcaaaat gggccgatgt ggacatggtt gtctatgtgg gatgtggcga
    22321 gcgtggaaat gagatgacgg atgttttaaa tgaattccct gaattgattg accctaatac
    22381 aggagaatcg ttgatggaac gtacggtatt gattgctaat acatcaaaca tgccagtagc
    22441 agctcgagaa gcttctatct atacgggaat cacaattgcg gaatatttcc gtgacatggg
    22501 ttatgacgtt gctatcatgg cagactccac ttcacgatgg gcagaagcac ttcgtgagat
    22561 gagcggacgt ttggaagaaa tgcctggtga tgaaggttat ccagcttatc ttggttcacg
    22621 gcttgcggaa tactatgaac gatctggaag agtcattgca ttaggatcag aaggtcgcga
    22681 aggaagtata accgcaatca gtgctgtatc cccgtctgga ggggatattt cggaaccagt
    22741 cacacaaaat acattgcggg tcgtaaaagt attctggggt cttgattcca gtttagccca
    22801 aaaaagacat tttccttcga tcgattggat acaaagctat tctctttatt caacagaagt
    22861 agggaagtat atggatcata tccttcaagc tgactgggca tccatggtca ccgagggcat
    22921 gcgtattttg caggaagaac agcagctgaa tgaaatcgtg cgtttggtag ggattgattc
    22981 cctttcagat aatgaccgtc tgacattaga agtggcaaaa tccattcgtg aagattattt
    23041 gcagcaaaat gcttttgatg atgtcgatac attcacttca agagagaaac aattcaaaat
    23101 gttgaagatg atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt taggtgctta
    23161 tttaagtgag atcatggacg gtaccgtatc gattcgtgaa aggatcggac gaagcaaata
    23221 tatcccggaa acagaattag aaaaactaga tcaaattatt ggtgaaatca agcaaacgat
    23281 gcaaacgatc gtttcagaag gaggcatgag caatgattaa agaatatcgt acgatcggtg
    23341 aagtcgtcgg tccattgatg gcggttgaaa aagttgctgg tgtcaaatat gaagaattaa
    23401 tcgaagtccg tatgcaaaat ggcgagattc gtcgtggaca agtattggaa gtccaagaag
    23461 ataaagcaat ggtccaaata ttcgaaggaa caagtggtat caatttacgt gactcttctg
    23521 tacgattttt aggccatcct ttagaactag gtgtatcgga agacatgatc ggtcgagtat
    23581 tcgatggttt gggacgaccg aaagataacg gaccagagat cctgcctgaa aagcatttgg
    23641 atattaacgg agaagtaatc aatccagtag ctcgggatta tccagatgag tttatccaaa
    23701 caggtatctc tgcaatcgat catttgaata cattagttcg tgggcaaaaa ttaccagtat
    23761 tttctggctc tggtcttcct cataaagaat tagctgctca gatcgcgcga caagcaacgg
    23821 tactcgattc atcagatgat ttcgccgtag tctttgctgc tattggaatc acttttgaag
    23881 aagctgaatt tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt tctgtcatgt
    23941 ttatgaattt agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt atggctttga
    24001 ctgctgcaga atatcttgct tatgaaaagg gaatgcatgt tttggttatc atgacagaca
    24061 tgaccaacta tgcagaggca ttgcgagaga tttctgcagc acggcgagaa gttccaggaa
    24121 gaagaggata tcctgggtat ctttacacga acttagctac tttattcgaa cgagcaggaa
    24181 gaatccgcgg actaaaagga tcggttactc aaattccgat tctaacaatg ccagaagacg
    24241 ataaaactca tccaatccct gacttgactg gttatattac agaaggacag atcatcttga
    24301 ctagagagct ttacaagagt gggattcagc caccaattga tgtactcccg tctctttcgc
    24361 ggttaaaaga taagggaact ggacccggaa agacaagaga agaccatgca gctacgatga
    24421 atcagctttt tgcagcctat gcacaaggaa aacaggcaaa agaattagcc gtagttttag
    24481 gtgaatctgc tttatctgat gtagacaaga tctatgcgaa gtttgctgag cggtttgaaa
    24541 aagaatatgt caatcaagga ttctacacga accgttcgat tactgaaaca cttgatctag
    24601 ggtgggaact gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa gacgacatgc
    24661 tggacaaata tctgccagaa gggaagtgag ttctgatggc acgattgaat gtgaatccga
    24721 cccgcatgga attgactcga ttgaaaaaac agttgacaac tgccacaaga gggcataaat
    24781 tattaaaaga taagcaagat gaactgatgc gtcaattcat cctgttgatt cgaaagaaca
    24841 accagctgcg tcaagaaatg gaagagtcta tgacagaagc aatgtctgat tttgtattgg
    24901 caaatgcttc gatcacggaa cctttcatcg aagaattatt tgttttgccg gcggaaaacg
    24961 ttgatttgtc tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg atgaattttg
    25021 aatatgatga acagctgaca gacacacctt tggaatacgg gtatttgaat tccagtgccg
    25081 aactggatcg gtcaatcgat cggtttacta ccctgcttcc aaaacttttg gaactgacag
    25141 aaatcgaaaa aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt cggcgagtca
    25201 atgcattaga atatatgacg atccctcaat tagaagaaac gatctattat attcgtatga
    25261 agctggaaga aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa aatatgggta
    25321 ccactgacta agtggtatct ttttttaaaa gaggaaaacg atgattaaac aaagcaaaca
    25381 aataaagaaa cgtttgccgg ctgttcagct tctcgcatta ggattttttc tcctgatttt
    25441 agttggcggc ggcctactta ccctaccatt ttttagtaat agtgggcatg ggacaaattt
    25501 tatcgatgcg cttttcactg caacttctgc tgtctgtgta acgggattga caacattgaa
    25561 tacagcagag cattggaaca gtgctggtca atttttgatc atgcttttga ttgagatcgg
    25621 cggtcttggt tttatgatgg ttccgattct atgctttgca ttgataaaga agaaagtcag
    25681 tctcagcaca cgtattatat taaaagaagc gctgaatcta gaagaagtat caggtgtgat
    25741 gaagctcatg ctttacatcc taaaattggc ctttgtgatc caattgattg gtacggttgc
    25801 tttatctttt gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt acagtttgtt
    25861 ccatgcaatc tctagttttt gtaatgcagg gtttgatttg ttaggagaca gtttagccaa
    25921 tcaccagcaa aatgtttatt tgatcatggt ggtatctgct ttgatcattt ctggtgggtt
    25981 aggcttcatc gtatggcgtg atttactgga atatcatcag aaaaagaaga tgatgattca
    26041 ttcaaaaatc gcattaaccg ttacaggtac ccttttatta ggcggattcg ttgtgttctt
    26101 tttcacagag cgcaatgggg caacgctagt ggaagggaat atgtttcaac gattggcaaa
    26161 cacctttttt atgagtgtga cacctagaac ggctggatat tattcagttg attacctcca
    26221 tatgagccat gcaggtctga ttttaacgat gtttttgatg tacatcggcg gaacatctgg
    26281 gtcgacggcc ggcggattga aaacaactac gttaggtgtg ttgctgatcc agatgcgttc
    26341 gatgttgaaa ggacgtacga gagcggaagc atttggccgt accatccgac aaggcgcagt
    26401 gttgcgggca cttactttat tttttgttac gttgacgttg tgtgtagtcg ccatcatgat
    26461 tttatcggtg actgaaacga tacctaaaac ttctggcatc gaatatattg cttttgaggt
    26521 cttctctgcg tttggtacag taggtcttac gatgggattg acgccagact tgactgcaat
    26581 agggaaattg atcattatag cgcttatgta cattggacga gttggtgtat tgactgtggt
    26641 cttctctctg atgacaaaag cacaaagaac acaatcaaaa tacaaatatc cagaagaaag
    26701 tgtcatgatt ggataatttc atcttctgac agaaacctag tgatttagta aaataagaaa
    26761 ataaaataat ttttcttgct tcttggatac tttaagtgca aaatgttcgt ttattttttg
    26821 aggcactacc cgttgttgaa cctcgggtag tgcctctttt ttattgaaag gaatcaataa
    26881 gatttgattc ctttatttat caataggtta taattcatat ttttttattt tttcttttac
    26941 gatacttagt ccagaagcta aaatttcaag gttattagag gatagggaaa ttcgaacgaa
    27001 tgcatctggt tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga taccgttagc
    27061 aagaaagtca ttctcaatta tcttagggtc aatctcagga tcaactggaa gaattcgata
    27121 gtaagggaca cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa gctgattggc
    27181 tctttttgta agacttaatt tagtttctaa gatcctattt gcttcatttg aatggaggat
    27241 ttgagtgatg acttccacat ctaatccaga agttttcaca tttatattaa aaattgcttg
    27301 ttctatcttg ctttggacat gtactggaac tacaagataa gcaactcgca atccagaaca
    27361 aataaatttt gtcattccag ctaagtagat tgtttggtta ggtaataaat tttggaaagg
    27421 aggaatgatc gtatgttgat agtaagttgt taaaaatgaa tggatatcat cttcaataac
    27481 ccatagacct tctttttcaa tcacttcggc taattgctct cgtcttttgt gagagatttg
    27541 aaaccctata ggattattac aagaaggcat aagaaagaca ccatgaatct tcttttttct
    27601 acattcctga atcaatatat caggtcgcat tccttgctcg tcaaaatcaa ttggaacgat
    27661 ctctaaatga tataactgag ccaattcaat gaaattagcg tatgtatatc ggtctgttgc
    27721 tattctatca cctggagaga aaagtgcaga aagtgtgata gccaatccat tttgaacacc
    27781 ggaaacaata gatagagatt ctaaggacgg aatgactcct tgagttttta accaatttgc
    27841 tgcagtagtc aattgaaaat ttgtgccttt gggatcttgg taattaagca aaccaacaag
    27901 atttgggtta ttactagttg attgaataaa cgggacaacg aatttattac attcttcaaa
    27961 agaactaacc agaccaaaat caatgatatg ttcggttact tcattggtag agatggtgat
    28021 ggactcaaag acattgggag aaataaatgt gccacttcca atattcgtat acagcaaacc
    28081 tttctcaatt ccatatttat aagccttgcc aacggtagta aaatttaaat ctaaataatc
    28141 agctagctct ctttgggagg gtaactgagt gtttttctgt aatttattct ttaaaatatc
    28201 ttgttctaat tgttgaatca acgatcgata ataaggacgc ttcaattttt gtttgtctgg
    28261 tcgccaagtt aacgggtaat tgtcataaga attaataggc attttctcca ctccttaaaa
    28321 ttgtatacca tacaatttta ccattgattg tattgatttt agaagttaat cttttgtgag
    28381 gagggaattg tctatgaaaa ttattaattt tgaggaagta gtagcttctt ttagttttga
    28441 agaagcgatt caagtaatga aaaaatgttt ttttgattat gaaagtggta aaatcagcca
    28501 aaatcctcga ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt ttgcgatgat
    28561 gcctgcttat ttgggggaga acagatattt tggcacaaaa gtcattaccg cttttccgga
    28621 taatcatcag tatcatttac cttctcattt aggagagatt ttcttgtttg atgctcaaaa
    28681 cggactaccc aaagcattga taaacgcaaa tgcagtcacc tggattcgaa cagctgctgt
    28741 atctgcgctg gcaacagatt acttagcaaa tccgcatgca gaaacacttg ctttaattgg
    28801 agctggtcaa caagcattgt cgcatttgac tgcgatttct accattcgtc ccatcactac
    28861 agtgtttgtt tacgatattt tagaagaaag acgaaatact tttatcgcta aagctaaaaa
    28921 acagtatcct aaaataacgt ttataaatgc aatatcagta gaagatgcag tacgtccagc
    28981 ggaaatcatt tgtacgttga cctctagtaa agaggcattt cttcaaaaag agtgggtaag
    29041 ggaagacgca catatcaatg ctgtgggcac atttactcct gatacaagag agataagtac
    29101 atcgttgatg gaaacttctc aaatctatgt agatgattac tcagctgcct taacagaaag
    29161 cggggacctg cttatagcca tggcagaagg agatttagag gaatcttgta ttgttggtac
    29221 attaggcgaa ttagtttctg agaaaaaaac attttataaa ggagaatcga ggccgacgat
    29281 tttcgacgca gttggtctag ctattgaaga tctttgttgt gcagaatata tttataacaa
    29341 attagagaaa ggggctttaa aatgaactta gattattacg tagttgatgc atttgcagat
    29401 gaggtgttca aaggaaatcc agcagcggtt tatgtgctag aagaatggct gccagaaggt
    29461 acgatgcaaa aaattgccat tgaaaacaat ttatctgaaa cagcatttac agtgaagaaa
    29521 aaccaagaat ttgagcttag atggtttacg ccagatcgag aaatcgatct ttgtggtcac
    29581 gcgacattag caacagcatt tgttttattt aactattaca aaatacctga tgaaacgatt
    29641 aaattttcta ctcaaagcgg taatctattt gtgacaagaa ttaaagatta ttattacatg
    29701 gattttccta gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga agaagcgatt
    29761 ggggctaaga ttaaagaagc ctacttagca cgtgatttat ttttcgtatt agaagatgaa
    29821 aaaacggtgg ctaaattgca gccagatttt acagctatca aaaattttga attaggaata
    29881 ggagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac cttctttcca
    29941 aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct tattccttat
    30001 tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc aaggggcggg
    30061 gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc agttttgttt
    30121 tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa ttggtggaga
    30181 gtggcaacct tatagctaca atggttgtta aaaaaagacg caaacttttt caatttaatg
    30241 aaaagtttgc gtctcatttt aattattggt gtttgttttt tattcaatca agagtaaaat
    30301 gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt caatgacaaa
    30361 gtagtaagaa ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa gaccagcagc
    30421 tttatgttgc tcgtgtaatt tttctagatc atcagctgct atagcgatat gaccatagcc
    30481 gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt catagtcatc
    30541 cccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac ggcggcggct
    30601 ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat ctttcactcg
    30661 aacacatgtg tgagccattt tcatagattc atctctcctt ttccttttac tcaatcataa
    30721 caaaaagaag taaggaaata gttgatttgt gcttgttcat gagaaatttc tcatgtaaaa
    30781 tggatagaaa ataaaactgt taaggtggaa actattcatg gataaaccaa tttttggtga
    30841 aaaaaagcaa tttgctgatt atcaaacccg ttatgcagca tatattgtca tagaaagaga
    30901 tcagcagatt gcagtaatcg aggctccaaa tggtgctttt tttcttcctg gcggggaaat
    30961 tgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag aaatgggaat
    31021 tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact cgaattatcg
    31081 tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga agcgtatagg
    31141 agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag cgcttgtgaa
    31201 attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa ataaaaactg
    31261 aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt acatattttt
    31321 tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt gctgcttctt
    31381 tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca atcatggata
    31441 agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca aggtgctgtg
    31501 cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg aagtaatagg
    31561 gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc gcaattgctg
    31621 catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg tctggtgtca
    31681 tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg tggatcgtat
    31741 aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa tggagatgac
    31801 tgccgacacg gcgtgccatt acttcgatct ctaggaaatc ttcatgtgtc agtccgtgac
    31861 gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa gtaataacgt
    31921 aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct ggtaagggac
    31981 ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc gcttctttta
    32041 cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg atagcgacta
    32101 gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat gcgccattac
    32161 ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga tcacgcaagg
    32221 acggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct tgtcccgcta
    32281 atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca tttcgcaaaa
    32341 tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa ggtagatcgt
    32401 agctagaatt cacagcagtg taataaacag gattatatcc gaccttttct actaacaaag
    32461 tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat ttcagtggtt
    32521 tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat tcagaccatt
    32581 gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct aaagtaaagc
    32641 ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg gcatcttctt
    32701 gatacaactc gtgtgcgcga tgaagcagat gctccaaaat tacttccata ccacgggaaa
    32761 caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa tcttccactg
    32821 catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc acacgtaata
    32881 ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc cgaagcaaat
    32941 agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt gggttggggt
    33001 aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact cggttaagaa
    33061 tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct tcatgattcg
    33121 tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca tgcaacagtg
    33181 ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct attttttctt
    33241 ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa acgcctaatg
    33301 aatgagtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg cctaattgtt
    33361 taatgcgtcg tagacgttgg acttctttcg aattgatcaa atctaagatt acttgatgct
    33421 ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc agcatttgat
    33481 atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc cattctctgg
    33541 atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt agaatcctgt
    33601 aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt gacttctttt
    33661 acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg atccactgca
    33721 ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg cttcatgact
    33781 tgcccacgtt tttcctgatt accgttgaca cttaagtaca tcataacaga aatcccattt
    33841 ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa atcataagtt
    33901 ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg aaaagcgtat
    33961 ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt tgtgtcactt
    34021 tgggggaaaa tcaaagaaat attcaaaatt ccttcatctg atacgatccc taatccacct
    34081 gaatttctga tcactggagc gtaacctgca tcttccagtg tttggatccc gtcacttaga
    34141 aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg ccagaaatgt
    34201 aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa tgcaaaaggg
    34261 gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa tcgcagggtt cattcaaccg
    34321 gctcctttgt ataaattcat attttctaca atattatact ctaaaatcaa agagttgttt
    34381 tggaaaaaag gttttctgta aaaatggaca agttggttga caaaacaaaa gaaactagga
    34441 acaatgtagc tatgagaagt aaaggagaat gaataatgga attatcacaa ggaacaccga
    34501 tttcaatcaa attgcgtaca aaagtaaaac agcaagggga agtgcaagat ttttattttg
    34561 atttgaaagg gcaaatggtg acaattggcg atacgctgta catacgttat aaagagattc
    34621 aggaggagac gggcgatgag attcctgtaa cgatcaaatt agttccagat ggtcatgtac
    34681 agctgatccg tgcaggagaa atgcggatgc gtttgaaatt tggctataaa gaacggttag
    34741 aaacatccta tcgaacacca tacggtatga tccagattgc tacttttacg aaagaactgc
    34801 atgttagttt gaaagaccgt cccacagcag ggaaggttcg aatcgattat gatctgttca
    34861 tgggaccgga aaaaatcggc gaatattatt tgaccttgga ttttacagcg taaatataaa
    34921 agaaaagttt gattttttgt gtctgatttt gtatgataag gagtagactg tgaaaggacg
    34981 tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta tcaatgatcg
    35041 aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat ggatttttct gatttagcta
    35101 accagatcca aaactatctt gggaaatcag acagtgaaat tcgtgattca ctcgcacaat
    35161 tttatactga cttgaatatt gacggcagct ttatctcatt aggggataat cgttggggct
    35221 tgcgttcatg gtatgctatc gattcgatcg atgaagaagt aaaccatgga atggacgaag
    35281 aggatgaaga tactccacgt cgccgtaaac gcaagaaagt caacgccttt atcaatgatg
    35341 atgaagacgc gatcgattac aatgacgatg acccagaaga tacagatctg acagaagaag
    35401 atgacgatga tctgtttgat gacgatgatg atgaagatga agaaatcgct gcttataatt
    35461 ctgacttgca agaaatcgga gcagatgacc cgacagatga tgaagaagac ttgccaggaa
    35521 atatcgaaga agatttaagt atcatagacg aagacgatga tgatgaagat tatgaagagg
    35581 aagaattttc tgactctgaa aaagaataaa cattattttc ttacgagagg ttgtgacaga
    35641 gacgatcagc ctatcagaat aaggaggtgt gacacgattt ttgtcacacc ttcttttttg
    35701 tatataaaaa tcccaagtaa atacataaaa aataaaaaaa ttgaaacttt tagcgcacct
    35761 tgtacgtcat attaagtgag gcagaaagga gtggagaatg atttttactg gatataagaa
    35821 aaaacggcag atcgaaaaat accgtcacct gctgaatgga ctcatcgaaa ctgattatca
    35881 aaaaatgttc cgtatcacga tgaattacgt acatgataaa gaagaggcct tagatgtgat
    35941 gcaagatagt tttcataaag ccctgaacta ttttgaaaaa gaaaaagaga tcgagcattt
    36001 tagtgcatgg ttttatcgga tattgatccg cacggcatta gatagttggc gcagacaaaa
    36061 aaggacgcct gtggacttat ttgaaattca agatatcccc caacagttac ctttagaaac
    36121 ttccgttgca gagctccata tgattttaga taaaatagac agtccggaaa aagagattct
    36181 gatcctctac ttttttgaag gatttcgttt gaaagaaatt gcgactatct tggatatgaa
    36241 tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat gctttacgtc aaatcctaga
    36301 atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa tttaaaaaaa ctaaagaaaa
    36361 cttataaaga acaagcaatt cccaaagggc ttaaagagga gatccgccag cgttttatta
    36421 tcgaagagca atcatttata aaagcaaagc ggagaaagag acgtctccaa gggttcggag
    36481 tatctttggc tatcgtaatg attacaacaa gtagccttct ttttaatagt caagttcgaa
    36541 gctttgcaga agatttaccc atcttaggat cagtcattga attgatctta ggcgaacgct
    36601 ttactgatcg atctgaaaaa atcgatattc aggtaccgaa gatcaataca caaaatgaga
    36661 aagaaaataa aacaattcat ggattaaatc aaaaatactt tcgcgaagga caagcagatt
    36721 ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga tcattaccaa gttttgggag
    36781 attatcagaa aatcgtagac gataaccggt ttctcgtgat tgaaaggcag atcacccaaa
    36841 cagcagcaga tagccatgtt gaaaaaagat acgacacaat cgacaagaaa aacagtgtcc
    36901 agctctcact tccactttta ttcaaagatg acacctatct atctgttttg acaaaagaag
    36961 tcaaacggca gatggccgaa caagtcaagg aagatccatc taagtactat tggactgagc
    37021 aagatattca agaagggacg atagaaaaac caacacttgt tacgcctaca cgaagttttt
    37081 atttgaacaa agagcatcaa ttagttttga ctttttctca atacgaaatt gctcctggtt
    37141 atatgggtac accagaattt gtcattccta agtccgtgac aaaaacgatt ttggcatcgg
    37201 aagattattt ggatcattag acgagaagta gttgcgacta gcagtttcaa ttttcaagta
    37261 agtacaagta aataataaga agagaaaaag agaaagaaga gatatagtga aaagaaatta
    37321 tttagttatc agtattattg ctttatccgt gcttttgttt ttcgaaatta cttcggtggc
    37381 ctccttgatc accgaagtaa aaggacaaac acactcttcg gagcattcgt cacaatcttt
    37441 caaacaatca gaacaatcaa ccattaaaag ccaatcaact gaaacagcta acgtcaaaga
    37501 agccattcaa gaatcagcgg aaaaggtaca aagtcagtca tcacaatcaa cacaagacag
    37561 tacaaaaacg agccgagatc cggctgtcaa aagcgtcgca atcagctttg atgatggacc
    37621 aggagcgacc acaacaccgc agcttctgcg aatcttaaaa gaaaaaaacg tgcatgccac
    37681 ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa atcgtgaagc agacagctga
    37741 agcagggcac gagattggca atcacacata cgatcatcag aatctggcca ctctttctgc
    37801 ccagtcgatg acggaggaag tgacgaaagc agacactgag atcaaaaaag tcactggcaa
    37861 aacccccact tttgttcgtc cgccctatgg ttctgtcacg aatgtaggag caacgatcat
    37921 ccagcgaccg atcatcgaat ggtctgttga ttcagaagat tggaagacaa gaaatcctga
    37981 tttgattttg cagaagatcc aagcaacagt atacgatggg gcaattattt tattccatga
    38041 tatctatcct gaaacgattc gtgccgttcc tcaagtgatc gattatctga aagagcaagg
    38101 gtatcgcatc acgacagttg gggatttatt agggcatcca acggctgtcg agaactatta
    38161 tggcagaaac gatcatcggc cagttcagta agtgagacaa gtatctttaa taaataacac
    38221 attttaataa gtagttgaac acataactac cttacttgca aaggcttaac aatatctata
    38281 caggtacaac aaaaaactgt ccaccaattt tctttggcgg acagttttca cttaaaaata
    38341 aagaagcaac tattcgtaaa ttatgctaaa atagcagtag aacgcgtcct gagggaatcg
    38401 aacccccgtc tcaagaaccg gaatcttacg tgatatccac tacactaagg acgcaagcac
    38461 tgggataatt gtaactaaac agataaaaaa ttacaagggg attggacaaa aaaatgaaat
    38521 ttgagcaaaa tttttcacaa aaacagcagc agacacagaa actagccatg acgcaacagt
    38581 tgcaacagtc catccaagtt ttacaatata acagtgagga gctgttggca ttcgttgaga
    38641 atcaagcaat ggaaaatcca ttggtggaag ttgttgagcc ggaatggcag ccagattaca
    38701 taaaagcttc ttcatcttct tatgaaggag aagagacaaa ttacctaaat cagattcctg
    38761 atacaaaagg gtctttattt gattcattga tcgaacaagt ccatttgaat taccgagata
    38821 cttttttgag gaaaatcgtt ctttatcttg tagaatacat cgatttgaac ggatttttga
    38881 cgattagttt agaagaagcc ggaaaagaga ttggagctac gcctatccaa atgctcgatg
    38941 cgttgactct gattcagcaa ttagaaccag ctggagtagg tgcacgtagt ttgcaagaat
    39001 gtctcatgct tcaaacagaa cgagatgatt atgcgccgga attagcttat atcgtgttgg
    39061 aagaatgttt tgaggaactg gtggagcgaa aatggaaaga aatcgcacaa cgtttttctg
    39121 ttgatctaca tgctgttcag cagatattcg actatttaca aacgttgagt ccaagccctg
    39181 gccggatttt cgaccgtagc agcgaattgt acattattcc tgatgtccgg gtattagtgg
    39241 acgacgataa aaatgtacaa gttatctcaa accgaaaaaa tcagccaaat atacgctttc
    39301 aagagagtta cttcaaacaa atgtcacaac aagcagataa agaaactgaa aattatttaa
    39361 aagaacggaa acaggaattc gaatggttga agaaaacgat cctacaacgt ggggatacaa
    39421 ttttgcgggt tgctcaagtc attgtttccc ggcaaaaagc tttctttatc gacaaggaac
    39481 gtccaatcaa gcctcttacg ttaaaggaag ttgcaactga gatagatgtg catgaatcca
    39541 cggtcagtcg tgcagtcaac gggaaatatt tggaaacaga cttcggtgta tttgaattaa
    39601 aaaaattttt cactacacga attcctacga atagtacaga acagacagaa gacctttcag
    39661 cagatacagc gaagaaaaag ctgcaggaat tagtcgatca agaagacaaa aacaagccgt
    39721 tgtctgacca gaaattggtt gaattgctga aaaaagatga gatagcgatc tcaagacgaa
    39781 cagtagcaaa atatcgtgat ttattaggaa tcccgagttc atcaaaaaga aaacgttatg
    39841 ataactaatc agtcaaaaga ctagaaaaag tatttcactt tttctagtct ttttagttga
    39901 aaaacgagag caaaactgtt agacttacag tcgtgaggtt gagatttccc tttattgatg
    39961 tgaaattcga atagttaatt tcttttttta tcgtatatgg gtcgttttaa gtctacattg
    40021 gacgtaaagt gtccaactaa ggagatcgct atgctggatg aaataaaaat gattgaatct
    40081 gttgcaccag atattatcga agtgttgcaa gaacgctata aaattttacg aaatatctat
    40141 tggatgcagc cgattggacg tcgaagttta tcggaaagta tggggatcac agaacgagta
    40201 ttgcgaacag aaacagatct tttgaaaaat ctgaatttga tcgacacctc aaaaagcggt
    40261 atgacgctga caactaaggg cgaagaagtt tatcaaagcc ttgaaaactt catggatcag
    40321 cttttaggta cgcatcaaac cgaacaacag ttggcagaat attttggtaa ccaacgctgt
    40381 atcgttgttt caggaaacag cgaagagcag accaaagtgg ctgatgcatt cggcgaagcg
    40441 ctgagcgaag ccctagaccg actacttcca gaaggcgaga atatcatcgc tgtgatggga
    40501 ggaactacta tggctgtggt agccgaacaa ttgtctaatc tggaaaataa gaaacggcat
    40561 aacctgtttg tccctgcaag aggcgggatt ggtgaagcga taactgtaca ggcaaattct
    40621 gtcagtgcaa gaatggctgc taaagcaaac ggtaaccacc gtgcacttta tgttccggaa
    40681 caattaagtc tggcaactta caattcttta ctcaatgagc catctatcca agaagtctta
    40741 aacttgatta gtgaagcgaa ttgtgttatt catagtattg ggcgtgcatt acacatggca
    40801 gcacgtcgta agatgactga aaaagaatta gtcatgctga aacaagcaaa tgctgttgcg
    40861 gaatcttttg gctacttttt caatgaaaaa ggtgaagtcg tttataaggt ccctagaatc
    40921 ggcatagaat tgcaagattt ggaaaaagtc ccgatcatca tggcaatcgc gggcggaaaa
    40981 tcaaaagcta aagcaattcg agcttatatg aaaaatgcac caaaacaaac gtggctcatc
    41041 actgatgaag ctgctgcaaa tgagatttta aaaggggtaa ccctttaaaa taaatatttt
    41101 tttgattttc ataaggagga aatcttaaat gacagttaaa gtaggtatta atggttttgg
    41161 acgtattgga cgtctagcat tccgtcgtat ccaagatgta gatggaatcg aagtagtagc
    41221 aatcaacgac ttgacagatg ctaaaatgtt ggcacacttg ttaaaatacg acacaactca
    41281 aggacgtttc aacggaacag ttgaagttca tgaaggttca ttcaacgtaa acggaaaaga
    41341 agttaaagta ttagctaacc gcaacccaga agaattacca tggggcgaac taggcgtaga
    41401 tatcgttcta gaatgtactg gtttcttcac ttctaaatca gcagctgaaa aacatttaac
    41461 agctggtgct aaacgcgttg ttatctctgc tcctggcgga aacgatgttc caacaatcgt
    41521 ttataacaca aaccacgaaa cattaactgg taaagaaaca gttatctcag gtgcttcatg
    41581 tactactaac tgtttagctc ctatggctaa aacattgaac gacaaatttg gtgttgttga
    41641 aggattaatg acaactatcc acgcttacac aggtgaccaa atgactctag acggacctca
    41701 tcctaaaggt gacttccgcc gtgcacgcgc tgctgctgca aacatcgttc ctaactcaac
    41761 aggtgctgct aaagctatcg gtttagtaat tcctgaattg aacggtaaat tagacggagc
    41821 tgctcaacgt gttcctgtac caacaggttc attaacagaa ttagtaacag ttcttgaaaa
    41881 agaagtaact gttgacgaaa tcaatgcagc aatgaaagaa gcttcaaacg aatcttacgg
    41941 atacaacaca gacgaaatcg tttcttcaga tatcgttggt atgacttacg gttcattatt
    42001 tgatgctaca caaactaaag taatgacagt tggcgacaaa caattagtta aaactgttgc
    42061 ttggtacgac aacgaaatgt catacactgc acaattagta cgtactttag aatacttcgc
    42121 taacttataa gatagacaaa cgtacaagtg taaattatag acatgtacat ttaaaagcgg
    42181 ggaagcaatc gcgcttcttc cgcttttttt gataataagt ttattcagga ggaaataaaa
    42241 tggctaagaa aacagtgaaa gatatcgatt taaaagacaa aaaagttctt gtccgtgttg
    42301 acttcaacgt tcctttgaaa gacggcgtga tcactgacga cactcgtatc aaagcagcat
    42361 tgccaacaat caactacgtt ctagaacaag gcggaaaagc aatccttttc tctcaccttg
    42421 gacgtgtaaa aacagaagaa gataaagaag gaaaatcttt agctccagtt gcaaaacgtc
    42481 taggcgaatt attaggtaaa gaagtaacat tcgtacctga aactcgcgga gaacaattag
    42541 aagaagcaat ccgcaacatg aatgacggcg acgttgtcgt attcgaaaat acacgatttg
    42601 aagatattga cggtaaaaaa gaaagcaaaa atgatacaga actaggtaaa tactgggctt
    42661 cattaggcga tgtatttgtt aacgatgctt tcggtacagc tcaccgtgca catgcttcta
    42721 acgtaggtat cgcttcaact ggtatcccaa cagttgctgg attcttgatg gaaaaagaaa
    42781 tcaagttcat cggtgaagca gtagaagaac caaaacgacc aatgattgca atccttggtg
    42841 gcgcaaaagt ttctgataaa atcggcgtaa tcgaaaactt gattcctaaa gcagataaaa
    42901 tcttgatcgg tggcggaatg acttacacat tctacgaagc aaaaggaatc aagatcggta
    42961 actctctagt agaagctgat aaagtggaac tagcaaaaga attaatcgaa aaagcaggcg
    43021 acaaacttgt cttgccaatc gacaatgtct gtgcaccaga attttcaaat gatgttgaaa
    43081 cacaagtaat cgaaggagac attcctgatg gtttgatggc tttagacatc ggcccagcat
    43141 cagtaaaatt atttgctgat acattacaag gtgcaaaaac agttgtttgg aacggaccta
    43201 tgggtgtatt tgaaatgagt aactttgcta aaggtacaat cggtgtttgt gaagcaatcg
    43261 ctaacttaga aggcgcaaca acaatcatcg gtggcggaga ctcagctgca gcagcagaac
    43321 aattaggatt tgctgacaaa ttcacacaca tctcaacagg tggtggcgca agcttggaat
    43381 tgcttgaagg taaagaatta ccaggactag cagcaatcaa cgataaataa acgaccaagt
    43441 ttgctcaatt ctgacgagta atcgatcaaa aggtgaaggg agttttcatg actccctagc
    43501 catttttcaa taaattaagg agtgtgttcc tcaatgcgta aacctattat cgcaggtaac
    43561 tggaaaatga acaaaactgc atcagaagca aaagaatttg ctgaagcagt aaaaaacaaa
    43621 atcccttcaa atgacaaagt tgattcagtc atcggatctc ctgcattatt cttacaagaa
    43681 ttaatgacat cagctgaagg tacagaacta aaaatctcag cacaaaactg ctactgggaa
    43741 aactcaggtg cgttcactgg tgaaacttca ccagcagctc ttgctgatct aggtgttcat
    43801 tatgtgatca tcggacattc agaacgtcgt gaatatttcc atgaaacaga cgaagacatc
    43861 aataaaaaag caaaagctat ctttgcaaat ggtatgacac caatcctatg ctgcggcgaa
    43921 tctttagaaa cctatgaagc aggcaaaact gctgaatgga tcgaaggaca aattacagct
    43981 ggtttagctg atctttcagc tgaacaagtg tcaaacatgg ttattgctta tgaaccaatc
    44041 tgggcaatcg gtactggtaa atcagctgac gcacaaatcg ctgacgaaat ttgtggagtt
    44101 gtacgtcaaa cagttgaaaa actttatgga aaagaagtat cagaagcagt tcgtatccaa
    44161 tacggcggtt ctgtaaaacc agaaaatatt gctgaataca tggcaaaaga aaatgttgac
    44221 ggtgctttag tcggtggcgc aagcttgcaa gcagattcat ttttgtcatt gttagacgct
    44281 gtaaaataat tgttgaaaat ttgcgaaaaa tgcaagaaat gtttgcacta atgagcaaaa
    44341 ttaactaaaa tcgtatatga gggaaatccc ttaggaaaaa acaaactcaa aggagagaca
    44401 aaacatgtca attattactg atgtttacgc tcgcgagatc ttagactcac gtggtaaccc
    44461 aacaatcgaa gtagaagtat acacagaaag cggagctttt ggccgcggaa tggttccttc
    44521 aggtgcttca actggtgaat acgaagcagt tgaattacgt gacggagaca aagctcgtta
    44581 tggcggtaaa ggtgtaacta aagctgtcga caacgtaaac aacatcattg ctgaagcaat
    44641 cattggctac gatgttcgtg accaaatggc tatcgacaaa gctatgatcg ctttagacgg
    44701 aactcctaac aaaggtaaat taggtgcgaa cgctatttta ggtgtttcta tcgctgttgc
    44761 acgtgcagct gctgactacc tagaagtacc tttataccac tacctaggcg gattcaacac
    44821 aaaagtattg ccaacaccaa tgatgaacat catcaacggc ggatcacatg ctgataactc
    44881 aatcgacttc caagaattca tgatcatgcc tgttggcgct ccaacattca aagaagctct
    44941 acgtatgggt gctgaagtat tccacgcatt agcttcaatc ttgaaagctc gcggattagc
    45001 aacttctgtt ggtgacgaag gtggatttgc tccaaacctt ggttcaaacg aagaaggttt
    45061 tgaagtaatc atcgaagcaa tcgaaaaagc tggctatgta cctggtaaag acgttgttct
    45121 tgctatggat gctgcttctt cagaattcta cgacaaagaa aaaggtgttt acgtactagc
    45181 tgactcaggc gaaggcgaaa aaacaactga cgaaatgatc aaattctacg aagaattagt
    45241 ttctaaatat ccaatcatct caatcgaaga tggattagac gaaaacgact gggacggatt
    45301 caagaaatta actgacgtat taggcgacaa agttcaatta gttggtgacg acttgttcgt
    45361 aacaaacact caaaaattat ctgaaggtat cgaaaaagga attgctaact caatccttat
    45421 caaagtgaac caaatcggta cattaacaga aactttcgaa gctatcgaaa tggctaaaga
    45481 agctggctac acagcagttg tatctcaccg ttctggtgaa acagaagatt caacaatctc
    45541 tgatatcgct gttgcaacaa atgctggcca aatcaaaact ggttctctat cacgtactga
    45601 ccgtattgct aaatacaacc aattactacg tatcgaagac caacttggtg aagttgcaga
    45661 atacaaaggt ttgaaatctt tctacaactt aaaaaacaaa taattctttt ctaatgaaga
    45721 tacatgatcg actattgaaa gatagtcgag tatgatcact tagttaagta atcgaactct
    45781 ctacttgctg aaatatcagc aggtagagag t
//
LOCUS       DBKOIJ010000004        45367 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000004 DBKOIJ010000000
VERSION     DBKOIJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 45367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.004"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(1..1596)
                     /locus_tag="ACV5NJ_000153"
                     /pseudo
     CDS             complement(1..1596)
                     /locus_tag="ACV5NJ_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1919..2398)
                     /gene="rlmH"
                     /locus_tag="ACV5NJ_000154"
     CDS             complement(1919..2398)
                     /gene="rlmH"
                     /locus_tag="ACV5NJ_000154"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HHY6484286.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2826..4115
                     /locus_tag="ACV5NJ_000155"
     CDS             2826..4115
                     /locus_tag="ACV5NJ_000155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HHY6484287.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4154..4759)
                     /gene="ytpR"
                     /locus_tag="ACV5NJ_000156"
     CDS             complement(4154..4759)
                     /gene="ytpR"
                     /locus_tag="ACV5NJ_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HHY6484288.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4964..5428)
                     /locus_tag="ACV5NJ_000157"
     CDS             complement(4964..5428)
                     /locus_tag="ACV5NJ_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HHY6484289.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5450..5770)
                     /locus_tag="ACV5NJ_000158"
     CDS             complement(5450..5770)
                     /locus_tag="ACV5NJ_000158"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HHY6484290.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5914..6993)
                     /gene="pepA"
                     /locus_tag="ACV5NJ_000159"
     CDS             complement(5914..6993)
                     /gene="pepA"
                     /locus_tag="ACV5NJ_000159"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HHY6484291.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7193..7543
                     /locus_tag="ACV5NJ_000160"
     CDS             7193..7543
                     /locus_tag="ACV5NJ_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HHY6484292.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7766..8713
                     /locus_tag="ACV5NJ_000161"
     CDS             7766..8713
                     /locus_tag="ACV5NJ_000161"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHY6484293.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8770..10188)
                     /gene="pepV"
                     /locus_tag="ACV5NJ_000162"
     CDS             complement(8770..10188)
                     /gene="pepV"
                     /locus_tag="ACV5NJ_000162"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HHY6484294.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10337..11182
                     /locus_tag="ACV5NJ_000163"
     CDS             10337..11182
                     /locus_tag="ACV5NJ_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HHY6484295.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11186..11626
                     /locus_tag="ACV5NJ_000164"
     CDS             11186..11626
                     /locus_tag="ACV5NJ_000164"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HHY6484296.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11676..11870
                     /locus_tag="ACV5NJ_000165"
     CDS             11676..11870
                     /locus_tag="ACV5NJ_000165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484297.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12006..12692
                     /locus_tag="ACV5NJ_000166"
     CDS             12006..12692
                     /locus_tag="ACV5NJ_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6484298.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13100..13789)
                     /locus_tag="ACV5NJ_000167"
     CDS             complement(13100..13789)
                     /locus_tag="ACV5NJ_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484299.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13764..15893)
                     /locus_tag="ACV5NJ_000168"
     CDS             complement(13764..15893)
                     /locus_tag="ACV5NJ_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HHY6484300.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15941..17278)
                     /locus_tag="ACV5NJ_000169"
     CDS             complement(15941..17278)
                     /locus_tag="ACV5NJ_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HHY6484301.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17530..17889)
                     /gene="rplT"
                     /locus_tag="ACV5NJ_000170"
     CDS             complement(17530..17889)
                     /gene="rplT"
                     /locus_tag="ACV5NJ_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HHY6484302.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17942..18142)
                     /gene="rpmI"
                     /locus_tag="ACV5NJ_000171"
     CDS             complement(17942..18142)
                     /gene="rpmI"
                     /locus_tag="ACV5NJ_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HHY6484303.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18214..18738)
                     /gene="infC"
                     /locus_tag="ACV5NJ_000172"
     CDS             complement(18214..18738)
                     /gene="infC"
                     /locus_tag="ACV5NJ_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HHY6484304.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18782..18907)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19058..19690
                     /locus_tag="ACV5NJ_000173"
     CDS             19058..19690
                     /locus_tag="ACV5NJ_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HHY6484305.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19692..20597)
                     /locus_tag="ACV5NJ_000174"
     CDS             complement(19692..20597)
                     /locus_tag="ACV5NJ_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HHY6484306.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20682..21341)
                     /locus_tag="ACV5NJ_000175"
     CDS             complement(20682..21341)
                     /locus_tag="ACV5NJ_000175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HHY6484307.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21657..22601
                     /gene="mvk"
                     /locus_tag="ACV5NJ_000176"
     CDS             21657..22601
                     /gene="mvk"
                     /locus_tag="ACV5NJ_000176"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HHY6484308.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22594..23571
                     /gene="mvaD"
                     /locus_tag="ACV5NJ_000177"
     CDS             22594..23571
                     /gene="mvaD"
                     /locus_tag="ACV5NJ_000177"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HHY6484309.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23584..24669
                     /locus_tag="ACV5NJ_000178"
     CDS             23584..24669
                     /locus_tag="ACV5NJ_000178"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HHY6484310.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24666..25730
                     /gene="fni"
                     /locus_tag="ACV5NJ_000179"
     CDS             24666..25730
                     /gene="fni"
                     /locus_tag="ACV5NJ_000179"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HHY6484311.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25979..26782)
                     /locus_tag="ACV5NJ_000180"
     CDS             complement(25979..26782)
                     /locus_tag="ACV5NJ_000180"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HHY6484312.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26802..27644)
                     /gene="kduI"
                     /locus_tag="ACV5NJ_000181"
     CDS             complement(26802..27644)
                     /gene="kduI"
                     /locus_tag="ACV5NJ_000181"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HHY6484313.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27660..28667)
                     /locus_tag="ACV5NJ_000182"
     CDS             complement(27660..28667)
                     /locus_tag="ACV5NJ_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HHY6484314.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28674..29318)
                     /locus_tag="ACV5NJ_000183"
     CDS             complement(28674..29318)
                     /locus_tag="ACV5NJ_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HHY6484315.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29418..30176)
                     /locus_tag="ACV5NJ_000184"
     CDS             complement(29418..30176)
                     /locus_tag="ACV5NJ_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HHY6484316.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30303..31454)
                     /gene="fucO"
                     /locus_tag="ACV5NJ_000185"
     CDS             complement(30303..31454)
                     /gene="fucO"
                     /locus_tag="ACV5NJ_000185"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HHY6484317.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31536..31847)
                     /gene="rhaM"
                     /locus_tag="ACV5NJ_000186"
     CDS             complement(31536..31847)
                     /gene="rhaM"
                     /locus_tag="ACV5NJ_000186"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HHY6484318.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31849..32694)
                     /gene="rhaD"
                     /locus_tag="ACV5NJ_000187"
     CDS             complement(31849..32694)
                     /gene="rhaD"
                     /locus_tag="ACV5NJ_000187"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HHY6484319.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32708..33964)
                     /gene="rhaA"
                     /locus_tag="ACV5NJ_000188"
     CDS             complement(32708..33964)
                     /gene="rhaA"
                     /locus_tag="ACV5NJ_000188"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HHY6484320.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33961..35421)
                     /gene="rhaB"
                     /locus_tag="ACV5NJ_000189"
     CDS             complement(33961..35421)
                     /gene="rhaB"
                     /locus_tag="ACV5NJ_000189"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HHY6484321.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35574..36443
                     /locus_tag="ACV5NJ_000190"
     CDS             35574..36443
                     /locus_tag="ACV5NJ_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHY6484322.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36569..37468)
                     /locus_tag="ACV5NJ_000191"
     CDS             complement(36569..37468)
                     /locus_tag="ACV5NJ_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6484323.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37633..38457)
                     /locus_tag="ACV5NJ_000192"
     CDS             complement(37633..38457)
                     /locus_tag="ACV5NJ_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HHY6484324.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38468..39763)
                     /locus_tag="ACV5NJ_000193"
     CDS             complement(38468..39763)
                     /locus_tag="ACV5NJ_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HHY6484325.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39779..40258)
                     /locus_tag="ACV5NJ_000194"
     CDS             complement(39779..40258)
                     /locus_tag="ACV5NJ_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HHY6484326.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40264..41292)
                     /locus_tag="ACV5NJ_000195"
     CDS             complement(40264..41292)
                     /locus_tag="ACV5NJ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HHY6484327.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFRETVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41309..42415)
                     /locus_tag="ACV5NJ_000196"
     CDS             complement(41309..42415)
                     /locus_tag="ACV5NJ_000196"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HHY6484328.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42644..45241)
                     /gene="adhE"
                     /locus_tag="ACV5NJ_000197"
                     /gene_synonym="adhC"
     CDS             complement(42644..45241)
                     /gene="adhE"
                     /locus_tag="ACV5NJ_000197"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HHY6484329.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13125 a         9658 c         8240 g        14344 t
ORIGIN      
        1 aaactaatta aaagaaagag gtaactacat aaactaatta aaaaagctaa cttcatagtt
       61 ggaggataaa tggaagaaac ttcaatgatt gtttctcctt gtgttccttc aatcgacact
      121 agtcctatta tcaatctctc ttttttgtcc atttactttt gcgactgttc cccaataatc
      181 aaatacagga agcgttattt tttgtccttt agattcatgt actgtataga ctattttcga
      241 atccatcact tgataaggca tagtatgcca ttccttgcca attcttactt catgattctg
      301 caaatgttct ttgtattgta atgcttccgt gggtgcatag tcataaagtc ctccgggaag
      361 gagtgtcgtg taacgaataa gattttcttg attcattacc aagtgatctc tctgagtgac
      421 aactttatgt tcatagtttg cttttgttgc agcagataca gccaacagta ataaaacaat
      481 tgccgttata ttaactactt ttctttgctt agctgatgtg ctcttcatga aaacgaacga
      541 taaaatcaac gagccaaaca gtacttgaaa gcctaagatt cgccaaggaa attgaatcat
      601 ttgaatgggt gtattttgta ataaagacca tggaaaaaat ttggtggata agagccaaga
      661 gactaccgca ctaaggtaaa tcactttttc tttgcgatta aatcctttaa aaaagaagag
      721 tggacagatc aaagaaataa taccaatgat tcctaccgta aaggttgtca ggtcattatt
      781 catcgcacca atcaaactat ccgagacatt taatgcttgg tgttgtaagg tcgttttatc
      841 tggctcatta atcgattgaa ccatgatttg tcggaacatc ggataccaaa agtatgctgt
      901 taaaagaatc gtcattccta ctgcctttga caactccaca atcaaccgga tgctttcttt
      961 ttttcttgtt tcacttacta caaagccgcc taagactagt aagccaacgg ctattacact
     1021 cataaaagtg gaaagaacat gtgtataaat gagtaagctc atactgactg ctaatggaat
     1081 tgcacatggc tttttctcat aaatcagtgc gtaagcataa tacataacta tcggtataac
     1141 cgtaagtgca atcgcttcag caatagcaga acgatgataa atatcaatcg tacggtaccc
     1201 ataaaatgta tataaacagc taaatataaa agccgcatcg attcttttta aaaattttag
     1261 tccataatga aacatcagca tgatagtaca aacattcaat agccaaacat ataagatata
     1321 actaacgatt aaattattac ttatccaata aaaaatgact gccggaaaaa aggtcaagta
     1381 gggataaaaa taattcactc cgctcccata atgattaaac gtcgtaaaat tgattggacc
     1441 agaaaaaatt gaagataacc ctttgactct agcaaaatgg aaggcaatat cccccgatgt
     1501 agaaatagta tgaacatttc taaaataatt gattgaataa agtccagcaa taattgctag
     1561 gagaatggac aatattattt ttttctttac ttgcattact taattatata ctcctttcat
     1621 ggattgcttt atctagtata acttaaattt taaataaatc gttatttatt tttactctca
     1681 cttaaaaaag cactaaaatt ataagagagg gaggtaatgg tctgaacacc aagaattgtt
     1741 ccaacatcga attgcttaac taatgagttc aaagcttaat tatgtggctc ttatatttac
     1801 cgagcgtgga ttgcgatctc tttagcgaaa caaacatctt tcataaaaaa aacagccata
     1861 tccgctttct tggatatgac tatttactac tactttacta tctttcattc ttcttgtctc
     1921 acttatgata aggcgccccc gcattgatcc ggaaggcacg gtagatttgc tcgacaagga
     1981 tcagacgcat caattgatgt ggatacgtca tttttccaaa agagatctga gcattgcttc
     2041 ttttcatgac ggcttcgctg agtcctaaag agccgccgat aacaaaaaca aggtggcttt
     2101 ttccttggat acctaattgg tcgatttgtt tagcaaaaga ttcacttgta ggatttttcc
     2161 cttcaattgc taaggcatag acgtattctt gttctttgat tttcgctaag atccgttccc
     2221 cttctttttc ttttacttgt ctcatttgcg cttcgcttaa attctccggg gctttttcgt
     2281 caggaacttc gatcaattcg atttttgcat aggcattcag ccgtttgaca tactcattga
     2341 taccttggat caggtatttt tcttttaatt ttcctactga gataattttg atattcatgt
     2401 tttctctcct ttggtcttca tgctcacttt gtttattata atcaattttt tcgtctatat
     2461 gtttattttg gctatttttg ttaaaaagat ttccccaaca gttatgcaca acatccacag
     2521 gtttatccac agaaaacagt gttattttac cttatttcat cacattcatt aggcaaacat
     2581 acgttttata agaatttatc cactttatac acaggctgtg gatatgtttt atcccttatt
     2641 tttccaatcc tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa tcaaatattt
     2701 tcatctgaaa acaatgattt ttcctaaagt ttttcataaa aagttcacag tctcaaagta
     2761 gactaaagtc aacaaataag actatactaa cagtagataa atgaatgtaa ttggaggagg
     2821 aacacatgga tagaaaaaat gtgacaccaa agatgaaaaa aagtaaaaat aatagcctct
     2881 ggcgtaagtt aggtctgggg cttgttggag ggatcgttgg aggactactg acagctggca
     2941 ttttttatgc agcgatgggc actggcaaca atagttctac tgctacaagc ggcaatcaga
     3001 attctgccgg tgaaacagtt gtggaaaatg tcaaagtcaa tgtagattct gatatcacca
     3061 aagcggtaga taaagttcaa gacgctgtcg tttctgttat caacttacaa agtcaaaacc
     3121 aatcaagcgg ttttggcgga ctattcgggc agcaagaaga aagtagcagc tcagatgata
     3181 gtaatttgga agcttatagt gaaggaagcg gcgtgatcta caaaaaagac gggaatactg
     3241 cttatgtagt cacgaataat cacgtagtag atggccagca aggtcttgaa gtcttgatga
     3301 aagacggtac gaaggtaaaa gctgaattag ttggaacaga tgcttattca gatttagcgg
     3361 tgttgaagat caattcagac aaagtcgaaa cagttgcctc ttttggtgac tcaagtgctc
     3421 tgaaggttgg tgagcctgcg attgcgattg gttccccact aggatctgaa tatgcaaatt
     3481 ctgtcacttc aggaatcatt tcttccttga accgacaagt gacaagtacg aatgaatcga
     3541 acgaaacagt caatatcaat gcgatccaaa cagatgcagc aatcaaccct ggaaattctg
     3601 gtggtccttt agttaatatc gaaggacaag ttatcggtat caactcaagt aagatcgcaa
     3661 gtacttctga atctacttct aatgtaagcg tagaaggaat gggatttgct attccaagta
     3721 acgatgtagt gaatatcatc aatcaactgg aaaaagacgg aaaagtgaca cgtccagctc
     3781 taggtatcac aatggtcgac ttatctgccg tttctactca gcaacaagaa caaatcttga
     3841 agattccttc atctgtaact aacggtgtca tcgtgacaaa tgtccaaaca gctacaccgg
     3901 ctgaaaaagc tggattgaaa caatacgacg tcattactaa gatcgatgac aaagaagttt
     3961 cttctggtgt agaacttcaa tctgctttgt ataagaaaaa agtgggagat tctgtcaaaa
     4021 tcacttacta tgaaggctcg aaagaaaaaa caactactgt tgaattgacg atcgatcaat
     4081 cggcattaaa acaaaatcaa aatagtgaaa attaataaaa aagaggttgt gacaaagact
     4141 tgtcacaacc tctttatttt ggaaatgctt ctccgactac ggcatcctca ggcaatacta
     4201 agattccttt ttttgctgga gcatttggca aatgcaattc acgagcggaa cagatcattc
     4261 catagctatc tacccctcgc aaagttcctg gccagatcat cattccatct ggcatcatag
     4321 ctcctggttt tgcaacgaca actttttgtc cagcttcgat attgggtgca ccgcaaacga
     4381 tttgcaatac tgtaccattg tctacttctg tctcagtaat cgataaatgg tcagaatcag
     4441 gatgtgggac gcatgttttt acgtacccga caacgatctt cggttcatga tcagcttcaa
     4501 gctgttcttc gaatcctgct tctttgatca atcgattcaa tttcgctaat tcctcatctg
     4561 ttaagcttac ttggccgttc ccttcgattg ttagatgatc ggagatatgg aaaaagttcc
     4621 aagcaacaac ttgaccttgt tcatctttga tccgagcaac attccatttg cgttcaacag
     4681 tgttttctgt tcctttatcg tcggctataa taaccatcaa ggtgtctccg acgtgtgctt
     4741 tgttataagc aaaaatcatg ttctttcctc cattatcctt tgctgagatt tatcttaaca
     4801 aaaaattgca ttcactgcat gaaatttttt tcatgcttta tttccgaaga agcctcttct
     4861 ggaacaccat ttattcgtaa caagggactg tgacaagact tttgtcacag tccctttact
     4921 gttttagtta attgattaaa aaggtcgtac aagttccact tgcttaatta cttggctgtt
     4981 cttcttcgtc agaaacgatc aagacatcac aaggtgaatg acgtatcacg tagctggcta
     5041 cacttcctgt cataaagcgt tcgactgcat ttagacctga ctgcccaacc atgattagat
     5101 cgatctcata tttttttggc aatttgactg ccatggattc acgaacagaa ccatatgcaa
     5161 taattccttc tatatttgtg taatcaacag atttcccata ttctttacat tcttcgatca
     5221 tttccttggc tgtttcagtt tgctgatcga ctagattttg atcaataggt gcatagccca
     5281 taaagttata aaattgctga tcgattacgt ttgctacata tactttccca ttattacggc
     5341 gagccacttc taccgctttt ttgaaagcag caagtgcttg ttcactaccg tcgataccta
     5401 ctaaaatatt cttatattct tgattcatct tgacccctcc taacagatgt cagccttttg
     5461 ttacactttc taggaacgat tcgatttcct gttttgtttt acgatcttta ttcactagac
     5521 gacctaattc ttttccgtct tggatcacga caaaacttgg gataccaaaa atgttccatt
     5581 cagcggctac atctatgtac ttgtcacggt ctacttcaat aaattgccaa tcagaaaagt
     5641 cattttcaat ttcaggcatt tgcggtttga taaaacggca gtctccgcac cagccggcag
     5701 taaagaaaaa tacactcttt ccagtttcta cgtagcctgc taattcttct aaactatttg
     5761 ggataatcat tctttgtcct ctactttctg tttatgtatt tttcaattaa attatacacc
     5821 tttctttcga actaacaaac attgatcctc tttttataaa aaaaatctgg caaaaagcaa
     5881 tcttttgcca gattttatag tttcactatc gcattatttt ccgtatacga tcgtatttac
     5941 tgttgattta tccaaacctt tcagtgtttg aatcaacatt tctcgtgcag cttcaaaatc
     6001 gcgaatactg aacatcgttt gatgcgtatg gatataacgt cccactacgc caatcactgt
     6061 acttgggatt ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc cttttgaaac
     6121 aaagtattga taagggatat tgtttgtttc agcgatatcc agcaagtact cgcgcaaacg
     6181 aggaagcatg atcaaacctg gatcttggat acgcatcaat gtaccttctc ctaaatgtcc
     6241 gaatgtgcca tttttcgtca ctgtatcatc tgctgcagag cagtctactg caaagaacag
     6301 atctggcttg aatttattga cagaagcttt agaaccacgc aagcctactt cttcctggac
     6361 attcgcgcct gcaatcaatg tatgacctaa tgtttcattt tgcaacgctt ctaaagcttc
     6421 taataccatt gtacagccat agcggttgtc ccatgattta ctgataatat tctttccgtt
     6481 ggctgttttg atcgtttcag cgtaaggaac gattgtgtct cctggaagga caccgtaact
     6541 cattgcctct tctcttgatt caaacccagc atcgaacaaa atatcggtta cttctactga
     6601 tttttgtccg cttgttcctc gcaataaatg tgggggaacg gaagaagaaa tacatggata
     6661 attcttacca gtagaggttt taagtgtaaa acgctgagca gaaacaacgt aaggattcca
     6721 gccgcctaga ggtacgactt taaaaagccc gttttcttgg atttgtgtca gcatgaatcc
     6781 gacttcatcc atatgtgctg cgaccattac acggggtgca tccgcttctt catgatggcg
     6841 cagaccaaaa atgccgccca aaccatctaa ctgtaactca tctaccagcg gttccatatg
     6901 actttccata taagcgcgaa catcttgttc aaaaccgctt gttccttgta attctgttaa
     6961 ctctttgatt cgttgaaatg ttttttcttc catatgctat tcgtctaaat ttcagactaa
     7021 actcctctca gacttatatt gatctgcgga taaaatatga ttttttctgc agaccgatcg
     7081 tatttattat agcttatttt ttctggtagc ggttatttat ttcaaagaaa atatgagagt
     7141 gtggtaaaat aagcacaagg agtaacaagt gagaagaagg aggtatgtcc acatgtatga
     7201 agaaagcgaa ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc taggattatt
     7261 gagcggggta gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag cagaccaagt
     7321 acttgaaaat atcaagtcat cttttctaaa agaaggtgca atcgaaggtt cttggatctc
     7381 ttttgaaaag gaacctatta ggaaattcgc cgttcggtcg agtgcttatc ggggaggtct
     7441 ttctcgaata gaagatggcg aaactgttta ttatgaattt ttagcagatg ctcatacagg
     7501 aaccgtatta gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca aaaaagtgag
     7561 agaacgaata cgcacccact attcaaaaga gaggtatcat gtccttcttt tgtagtgggt
     7621 ggttttttag atagagcttt ccaaacatat attccagtat tttcttttta aataaatgat
     7681 atttagcggg aaaaactagc atttcctttg attatgaggt atttttaatt tagcttttgt
     7741 tttctatagt taaggagagt gaaaaatgga acaacaagaa aaaattgctg ttctgattcc
     7801 ttgttacaat gaggaagcaa caatttcaac agtcattgca gactttaaac gagaattacc
     7861 agaagcagat atttatgtct atgataataa ttcgaccgat cgaacatacg aattggccgt
     7921 tcaaggtgga gcaatcgtaa aaaaagaacc tcgtcaagga aaaggaaatg tgatccgcca
     7981 aatgttcttt gatatcgatg ccgactatta tttaatggta gatggcgatg atacttatcc
     8041 tgcagaagct gtacatggtt tgctcgagaa attacgttct ggtgaagcag atatggtcat
     8101 tggtgatcgc ctatcaaatg gaacctactt tgatgagaat aagagacctt tccacgattt
     8161 tggaaataac cttgttagaa atacaattac tagaatgtat aaaacaaaaa ttcttgatgt
     8221 catgaccggc tatcgaggat tcaatcgtat ctttgtcaaa agttttccta ttatgagttc
     8281 tggttttcaa atcgaaacgg aattgacgat ccatgcttta gataaaaaat tcaagttcgt
     8341 tgaaatccca atcgattatc gtgatcgtcc tgaaggcagc gaatcaaaat tgaatacatt
     8401 ctctgacgga ttcaaagtga tcatgatgat tattaaaatg tttaaagatt ataagccttt
     8461 gcttttcttt gggatttgga ccatcttctt ctttttattt ggattggtca cggggattcc
     8521 tgtgattcgc gaatatatgc tgacaagctt tattacacgc atcccttctg cgattttaag
     8581 tactggattg atgactttag cacttctttc attagtaaca gggttgattt tggatacagt
     8641 cgtcacgaac gctaagaaag aatatgaatt gaatctatat catgtttatc atgaatacca
     8701 cagtagaaag taaaaaaagg atcaagactg caaaaataat ttgcaattct tgatcctctt
     8761 tttaattaat tatttcgcca aacgatagat ggcatctgca tagattgctg ttgctctgaa
     8821 caagtcatca agactcataa attcgtttgc ttgatgcatt gtatcagtat atcctgggaa
     8881 catcgctcca taagcaacgc cacgtttcaa caaacgtccg taagttccgc caccgatgat
     8941 ttgttcgtag cctttttctc cagtgtggtc ttcatagact tgcaataatg tagccaccaa
     9001 tggatcatcc attggtacgt aatgtggttc cattacgcga gcacctcgag taactgttgc
     9061 gccttcttgc cccactgttt gttcgatgtc actttgcaat ccgtctactg ttacaccttt
     9121 tggaaaacgg aaattcatat tgatgaagtt cgcttcctct gtaccatttt cttcaaaagc
     9181 aaatagtcca gcattcattg tcaatttgcc cattttttca tcttcataag cgactcctag
     9241 tttttctcca taaaaatctt catggacata agcagctgct acatggataa attgtttagc
     9301 agatcctagg aattcatagt tatctaagaa caatgccaag aaagaacctg cattgatacc
     9361 agattgtggg cttgcaccat gtgcgccttt accaactaat tcgattttta catacgtatt
     9421 gtccgcttca atcgttccgc ttactggatt tgcttcgatg aattggtaaa atgcttcttc
     9481 catcgcgata gctgcatctg cacttggtac ttgaagtgct gcagttgctg ttcctggcac
     9541 catattttcg cgaagtccgg aaacaaaaga tttcaagaca taatctcctg cattgtcgcc
     9601 tttgaagcgt aaagctaaag aaacatttcc tttttcacca ttgatgattg ggaattcagc
     9661 atctggtgag aaaccaaaat ctggttcttc ttcatgttcg aaataataag ccatatcgcc
     9721 ccagccgctt tcttcatcgc tgccgactac gaagcgaact ttttttgata gtttcaagtc
     9781 aagttcttta atgatcttca tcgcatagta agctgccata cttggacctt tgtcatcgct
     9841 tgatccgcgt gcaaagatct ttccatcctt gattactggt tcataaggat ctgtgtccca
     9901 gccatctcct gcaggaacga catccatgtg tccaaatatc cctagcgttt cgtctccttc
     9961 gccaagatca atatgccctg catagttatc gacatttttt actttaaatc catcacgttc
    10021 gccataagct aacatatgtt tcaatgcgtc gcgaggtcca ggtccaaatg gtgcatctgg
    10081 tgttactttt gagtcatcgc gctcgctgtt gacacgtaaa agatttttca agtcttcaag
    10141 caagtcttct ttacgtgctt ctacttcttt ttgccaatca attgtcataa agttaatcct
    10201 ccacttattt aagtcttcgt gttcatgata ccacaaatta cagaaaaaaa tagttcaaaa
    10261 aatctgacaa ttccttttct tatctgtttt tctctaaaaa aaatatacgc tataatgagt
    10321 aaaaggaggt ttttagatgc tgataaacga acaactagtg aaaagagtca tcacacctcg
    10381 tccaaaaatt tcacataaag gaacatttgg acgtatcgtt ttgattggcg gcaatgaaca
    10441 atatggcgga gcaatcatta tgagtgcttt agctgccgta cacagcggag cgggtctcac
    10501 gactgtagta actgatgaaa aaaatcggag tgctcttcac agtcatctgc cagaagcaat
    10561 gattgtagtt tggacggata ctgaccagtt tctttccgta ataaagcaag cagatgtact
    10621 cttgattggt cccggattgg gtacttcttc taagagtttg gaacgtttca aacaagtact
    10681 agaaaaccaa tcaaaagaac aatggctggt gattgatggc tctgctttaa gtttacttgc
    10741 tgctaatcat cttttgctac cctatcccga acaaactgta cttacacctc atcaaatgga
    10801 gtggcaacgg ttgagttctc taccgataca agaacaacgg gataaagaaa acaaaaagaa
    10861 gcaacaggaa atcggtgctg tgctcgtttt gaaaagtcat gagacaaaaa tttatggtca
    10921 ccagcacttc tacaccaatc ctttaggttc tgctgctatg gcaacgggcg gcatgggtga
    10981 cactttagct gggatgatca caggattttt agcccagttt cctcaaaatg accaaacgat
    11041 tgcagcagct atttatcttc atagtttcat tggagaccag ctggcaaaag aacaatatat
    11101 tgttttgccg acacaaatca gcgaagcact tccgaaatgg atgaattatt tttgtaaaaa
    11161 atcaatggaa aacgaggcat gagtcatgga gaaatttaca ggttatgtgc gagaaccttt
    11221 ttattatgaa acagatcaaa tggggattat tcatcacagc aattatatcc gctggtttga
    11281 agaggctcgg gtagccttat tggtacatct agggtttccc tataaaaaaa tcgaagaatc
    11341 tgggatcatc atccccgttc tggaagtaaa ttgcagctat aaagaaatga tccattacgg
    11401 agaaaaagtg agcatccatc caactatcca aaagtatacg gggacacggt tggattttag
    11461 ctacgaaatt gtgggaactg aagacaaaaa attaaaaact actggcagta gtaaacactg
    11521 ttttttactt tctgattcgc aacgattagt gaagctgagc aaagtcaatc cggagttaga
    11581 tcagttattc caagcgtacg cttctttgga tgatgtaaat tcttaataaa gaccgtctaa
    11641 gcatttttct tgtattgtgt cgagtagttc gtataatgaa aagaaaaaag caggtgatcc
    11701 ccatgtatgt cgttaaagta atgcacggtt acatcgataa aactggctgt cgcactcggg
    11761 aaaaaaactt agacaatctg ctcattttca aagacaaaaa agaatccgaa gcttttgcta
    11821 aaagaatcgg cggtcgtgtc aaaccgatcc aagaggtacg accagattga ttcaatattt
    11881 ttcttttcga ggctctaata ggcatgccta tcagggcttt ttttgcagac tgtgttttta
    11941 tgcccacacg atttgtaagc gatttatgtt ataatagatt atatctgcat agaaaggaag
    12001 gttaaatggt tatcaataat ttgcaacagc aagcttatga atctatacgt aaacaaatca
    12061 tttattccga gttagcacct ggaagtaaaa tctccgataa agtgttagaa gaacaactag
    12121 ctatcgggcg tacaccgatt caagaagcac tgatccaact gcgcaatcaa gagcttgtaa
    12181 aaaccattcc acagtccggc acttatgttt caaaaatcga tatccgatct gcttcattag
    12241 cccgttatac gcgggaaaag ttggaaaaac cgatcctgca agaatgcagt gcgaaaatga
    12301 ccaagcaaga tcaagctaaa ctagaaaaaa tactagaaca aaccgatcag gcagtaatcg
    12361 ctcaaaataa aaaagctttt tttgatttgg atactgattt tcaccgaact tgttatgaaa
    12421 ttgctggaaa acgagaaatt tgggattggc tagaaagtta tagtacgcac ttgaaccgtt
    12481 tcagatggtt acgtttgacg atttcagaat tagactgggg tcgagtatta gatgaacatc
    12541 agacaatgct tcaatcaatg attgatcata actttgatga agtcggattt ctttgcacga
    12601 tgcatttaca tatgattatc gaggagcaag aatacgtgat tcataattac cccgattatt
    12661 ttgaagatat tcaggacaga cccatcaaat aagaggtttt ggaatcagcg ttttgacctg
    12721 agtattagag aaagaaacgg caaaatagtt cctcatattt tgccgttttt tttgagctaa
    12781 taccgagggt cagctgattg cacaccgttt attgagaggt tgtgaaaaag ctgccctgca
    12841 tcaagtaata agaacagcca agtaaaaata gcttcttatg tttttaactt ggctggactt
    12901 attaccgcag atgcaagctt ttgaacaccg ttttccaaga ggtcgtgagc ctgccgttca
    12961 tctccgagtc acaaggaaca atttcaaaaa acagcttctc atgtttgttg aatattttga
    13021 cttgtgaccg aggagttgac aggagaacat cgtttattcg gaaaaaaagg actgtgacac
    13081 gacttttgtc acagtctctt tatttgtaat ctaaatcata ctccgtacat cttttttcta
    13141 ttttttttat ttcttctaag atcccttggc tacgaatttc attatatggc caaagtaaga
    13201 ctgcctctaa tacataaggc gaaaaatcgc tttgattcat ctcttttgaa aaactcatcg
    13261 ctgctatctc gctcaatgca cgaatgtttg attgatgtgt ataaaaaggc aaccgccaca
    13321 gcacaatttt ttcgctttgc gtataaattc ccttaacaga agcttcgatg tggtgaaata
    13381 gttcatgtcc aataatcaaa gcttgcgctg tttctttaga gatcgaagct agtcctgaac
    13441 attttaattt ttctaacggc tccgtcatca atcgaatccc ttcatcttct gcataagttg
    13501 caaaagtgat tcgttcgttt atttgctctg attcacagga aacgattgga acagccattt
    13561 gttcacaaat ttctcctaca tcggcaaaag ggtacttttt tttgatgcta catgccgttt
    13621 cgataccaca atcgattgat tgttgtataa gaattttctg cttggacaaa ggaatctttt
    13681 tccaaaataa atctcgccga aactggtaga atccccaaat ttcttcgggg atctgttgta
    13741 attcgtttag taactcttct aacttatgtt tgtttgatat ttccggcaac gacgatcact
    13801 tcttcctccg gttccatagt agatacttcc agatccatca aaagctgtag ttgttccaga
    13861 tccgtcgctg taaaatcaga gactcttgct cctaagcaga gataaaattc tggtcgggca
    13921 atcaattcag tttgataaac tgccatatct tcccaaagtt gttcgacagc tgtcatataa
    13981 ttggctgcag ttgttttcat acatgaagca tgtccacgtt ggaccttgat aaatcctttt
    14041 tgatcgatga tccgaagact tccagcattt tttgaacgat tctgttcgcc gcatacataa
    14101 aagaaaggtg tattttccaa taagcaaact tcatttttat ttaatcgcat atcctctgca
    14161 gcaagctcta gtgcttcttc tgttgtgatc tccttggtta gatcggttgc tttcacttcc
    14221 gtagaaccag tagcgatagc cgttactttt gaagtctgtg gatcgatttc tacgtggact
    14281 tcgatcgatt ctggtgtagc gcctgattca atcgctttat tcatcgcttc atttttcaac
    14341 gatcgtatat cttctttact tggcgaaggg atgatacgtt ctacaacatc tcggaccatt
    14401 gcaagtgcta caccaataga agaaatcact tcggcgtttt caggaatcga atatttcaca
    14461 cccattttat tactaaagta agtaatcaat gaggcagcgc cgccccctac acctacaaga
    14521 gaaatctgat ctttctccaa gtgatacttg tctgccaatt ctaaaatcac cggttcaatc
    14581 ttagcatagg atttttccat gatctgttct gcgatgtctt caaccgtcgt atgacaataa
    14641 tcagctaatg cttggatggc ttttcgtgca gatgggacat ttccataaga aaaatgttct
    14701 tcttgcacta atccaaggac attggctgca catgtatttg taatagtcac ttcttctccg
    14761 tcttccatca ctaccttgac gtaatcagca gggtctcctt ctttaggaga gaaaaattca
    14821 accttaggac ctttgatttt ttctgtctcc gtaaatactg cataatctag accggcaata
    14881 tgtgcagaac gtggaccgac atcgatgatt cctgactgat tagcccgtac catcgaaccg
    14941 cctgccacac ctagaacccg aacatctaat gaagagatat atgttgggtg gccacctaca
    15001 attgaataat cgattgctgg acgtccattt ttaatgaccc cgatattggt tgttgttccg
    15061 cctacttcaa aataaacacc attagacgct ctcaagtaca tcaaagagcc catgactgat
    15121 gctgctggac cggaaagcat ggttaaaaca ggtcgttttt tcatttcatt gatttccatt
    15181 actccgccgt ctccgcgcat aatcatcaaa gaaacattga cccctgcttc tcgtacggaa
    15241 tcttctgtag aggttgccgt atctagcatt tttggtaaaa tagatgcatt gattgctgct
    15301 gtccgagttc ttcttgtcaa tccatataat tttgtgatgt cagaagccat cgttgtaggg
    15361 atcgatttta ccgatgctgc ttcataaaca actcgttctg gttccccatt atctactcca
    15421 aatgccatag aagaaaccaa gacctccgct ctttctcttt ctaaagaaga aatcgtttct
    15481 gaaacacggt ctacatttaa gtgtttgaca ggtaagaatg cattgacaat ctctattttt
    15541 ttcttattcc ctaaatcgat atcgttcaat ctagtttgtc gttttgctaa aaaaccttct
    15601 aatccacctt ttgccatccc gattacgccg acttttgcta cgtctccttc aatcagtgca
    15661 ttagtcgctt gtgtggtaga atgtgcaaca aacacaacat cttctggact gatattattt
    15721 tccagcaaac aattttgaaa agactgaacg acacctgcgg ctactccacg gacatcatcg
    15781 tgggttgttt tcactgaaga ttttccgatg atttcgtgcg tcgcattatc gattgcgact
    15841 gctttcgtat gtgtaccgcc aacatcaatt cccattcgca caaaacgttt cattttcttt
    15901 tcctccattc atctaatcgt ttttatcgac gttttgttta ctaggcgttc ccgtacaata
    15961 agaacgtgat caggcataag atcgctccga tgatccaacc agttgggatg gatttcttca
    16021 tatattctcg agaagttact tttgtataac ctaatcccca agcaacccaa gattgtgtaa
    16081 tatctaaatg ctgaggagca atcgttgtga ttgcaaataa cgggaacaaa aaggctggcg
    16141 acatcgttgg attaacagct aatacgacag ctaaaatagc tgagcctgaa ccgactagat
    16201 tcattggacc gcggaaaaat cctagtggtg tcaatatagc aaatacgatt gccaagacta
    16261 atggtgattg cggtatccaa tctccaaaaa tagctgaaaa ataaggagaa gcatacgcag
    16321 ctgaattatt gaacatcgct aaggtcatca agaaaccaac cattggcgca acatctactg
    16381 cgccatctgt aaataatttc gcaaactgac gacagatttc tttataacct ccgcgcaatt
    16441 ttccagtaaa cagcaatgcc cagatacctg ctagaataaa acctaagatg attggaagat
    16501 ctaaaagaac gacccctaaa actggtaata atacggctaa ccatgagatc attggtgcgt
    16561 cgccaccttc agctggtacg tttgctgcca ttgcatagcg tttctttttg ttcattgata
    16621 tattagcgac cgttagtacc acaactaagc ttacaatcat cccaatcata ccaatttgga
    16681 aatagtcatt ataagtatag ctttcagccg ctggattgaa accagcataa atcgtttgat
    16741 attgtttgaa attgacgata ttagcaaaga ttccagccat aatcgagccc ataaatgaga
    16801 acagtgcaac cggtactgaa atacctaatg acatcaagat tggcaacaca ataactgcaa
    16861 tagaaatcac tggtccgatt cctgtcatcg acatgaatat aacagcggtt acaatacata
    16921 gtaagctcat cgtgattctt ggcttgtccc ctccaagttc aactaccttt cgaatcaggg
    16981 tagctgcgat tccgctatct actagaacac gcccgaaaaa ggcaccaaag aagacattta
    17041 ccaagatcga tttggcgtac tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa
    17101 ttactccttg cccttccatc gccgggtttg gtgaaaacgt gtttccgact agcgctaatc
    17161 ccatccatac agtagccatg atggcaaagc cgaccatcaa gttgtagcct tttacacagt
    17221 accagaccat gaaaaagaat gagataacta acaaaatacc aataataata tcactcatgt
    17281 tattcctccg tccattttgt ttttctgata agcaaactaa tatatcagtc aaactcattc
    17341 taacttatgt agttagcgct ttcattaaag ggtgaccata aatgttcagt tttcaattaa
    17401 aaaaccttaa ttatgctatt ttagacttat acgttattgt cctgtttcat ttgatggagg
    17461 attgtaaata aaaaaagagg aaagaacata acttttcgtt atcttctttc ctctttataa
    17521 ttgtttagct tatttagcca aagcttcttt tgcttggtca gccaatgcag taaatgctgc
    17581 tgcatcgtta acagccaagt cagctaacat tttacggttg atgtcgattt cagcaacttt
    17641 caaaccgtgc attaattttg agtagctcaa gccattcata cgagctgctg cgttgatacg
    17701 agcaatccac aatttacgga agtcgcgttt cttttgacga cgatcgcgat atgcgtagtt
    17761 gtatgaattc attacttgtt cttttgctgt tttaaataaa gtgtgttttg aaccgtagta
    17821 accttttgct aattttagca ctcttttacg acgtttacga gttactgttc cacctttaac
    17881 acgtgccatg tataattcct cctaataaaa ataaatcttc agattctgtt atttgtactt
    17941 cttatttcat ttttgctagt tgttggcgaa tacgtttgaa gtcgccacta gaaaccatgc
    18001 ttgctttacg caattgacgg cgttgttttt ttgttttgcc gtggaaacgg tgacttgtaa
    18061 acgcgcggaa gcgtttcaat ccgcctttac cagtacgttt gaaacgtttt gctgatccgc
    18121 ggtgtgtttt ttgttttggc atgactatat ttcctcctca aaatctttcg ttatccgact
    18181 atcggctgtc caacagccag ttagtcattc aaattactta ctgtcgtttt tcggtgccag
    18241 cgtcaagaac atgctgcgtc cgtccatttt cgctttttgt tccactgttg caatatccgc
    18301 agtttcttca gctaagcgat tcaagacttt ttgaccaatt tctttatggg taatggcacg
    18361 gcctttgaaa cggatagaag ctttcacttt atcgcccttt tccaagaact tacgtgcatt
    18421 acgaagtttt gtattgaagt cattcaagtc gatcgtagga cttaagcgaa cttcttttac
    18481 attgatcact ttttgttttt tacgcgcttc gcggtctttc ttttgttgtt cgaaacggta
    18541 tttaccgtag tccatgattc gcgcaactgg tggtttcgca ccaggggcaa ctaacacaag
    18601 atccaagttt gcttgttctg caatattcaa tgcttctgct tttgtcttaa ctcctaattg
    18661 ctcaccatct gaaccgatca atcgtaactc tcgtgcacga atgccgtcgt taaccatcat
    18721 atcctttgct atggtcattc acctccaaat attatgagag aaagaaaagc ttaccgaata
    18781 aacggcaata aaaaaacgag ttctcttaca gaacccgcgc gtacttcacc taatagaacc
    18841 agtgaaaact atctagccca gtgacattat caactaaggc gagaagcggg tgcttctgct
    18901 tttatttctc aaccatcata gtatagcaaa aactaatctt tttgtcaatg tttttcatac
    18961 agtttgtctt ccttttctga aaatgattag attagcagac tttcttgcag tgattcccgc
    19021 ttctttgcta tactaaagaa aaacgaggtg aacccacatg aaaaaaatcg aacctattcc
    19081 accttctctt cctcttctta caaagggaga gttttattta gaagatgagg agatcttctc
    19141 tggtcttctg atcaacgggg gtgaccaaag ctatctcgat gccaaaaata tggtattaag
    19201 agagagtcat atccaaaaac ttacgatgca aaaaacagtt ttacgtcatt ttgaatgcag
    19261 taatgtcatt tttgaaaaat gtgatttttc caatctggag tggattggtg gcagtttcca
    19321 ccaagtcgtg tttcatcaat gtaaattaac aggtacgaat tttgccgaaa gttatcttcg
    19381 agactgtact ttcactgatt gtattgcttc gatggcttcc tttagtagta caaacttgaa
    19441 agctgtctcc ttcgatcatt gtcaattaga agacagcgaa ttttatgagg tcacttggaa
    19501 aaaccttttt ttgtctgaca atcagttgac cggttcgaac tggtttcgaa cttctctgaa
    19561 gggattagac tttacgacta atttcttcac agcaatcgct ttatctcaag attctttggc
    19621 gggactaatc gtcaaccaag agcaagcact tgtgatcgcc caagcccttg gtttgcagat
    19681 caaagactag atcaacgaat ccgccgccac agtgcaataa gcatccaaat agataagacc
    19741 aaactgatga tcaaggcgat tccccatccg aaaacggaat gggtaaaggg taactggaga
    19801 ttcattccga agaatcctgt cacaatagtt ggaacggtca agatcagcga ccagaccgtc
    19861 aaaaatttca tcgtgtcatt caagttgtta ttcagcaaat tattataagt gccagagagc
    19921 tggtctaaaa tctgcgaagc taagttcgtc atttcaactg cttgtcgtgc ttcgatcagc
    19981 gcatcgtcca actcttcttt ttccttttct gacaacttgc gataaatagc caatgccttg
    20041 atttgttcta aaagtactac attttgtttt gttcctgtaa ctaagtaaac caaaccgatc
    20101 tctaaatctg aaagttgcag cagattcttg ttcgttgttt tttctcttaa tttcagatta
    20161 agccgctgac gttcactatt cacttcctcg attaaaggaa aaaaggaatc agaaatcaaa
    20221 aacaatgtac gaaacaataa actgtataca ctcatgtctg gcattcgttc caaaagcgat
    20281 cgaattagtc gaatcacgta ctcggtattc ggcgtggtaa atgtaaacaa accgtcttct
    20341 tttaaaataa aagcgatagg actagtttca taatgattat cgactttttg ctggtgcgga
    20401 acatttgata caagcagtaa cgcttcatca aaagaatcgt actctacgcg cgcccgttca
    20461 tttttatcta gagaataagt cagcatctcg tcactaatgc catattcttc ttttaagttt
    20521 ttaacagcta gcacatcgtt ttgctgtaca ttgatccaaa aattacggtc ttgattaaaa
    20581 tagtgttttt cccgcatact ctatcctcct gacatttgtt tccagtataa agaaaaaagt
    20641 cgctcaaata aaggtttacc ctttatttga gcaactgtaa atcaaatggc tactttggtt
    20701 tggcgaatct ctgctaattt ttcttcgatc attttcaaga cttcttcttt tgcttgagga
    20761 tcttccacaa aattatagcg gtctccatcg atttggattt ttggactttc tttgtattct
    20821 tcataccatt gatcgtaacg tctgttcaat tctttgtaat aatcgtatag acttggatca
    20881 aaggacaatt gttcatacga acgaccacgt ttttcgatac gctccaacat cgtatcaaaa
    20941 gatacacgaa tatgaactaa taaatccgga tgttttttat gggcagcata gggaagttct
    21001 tccatcatgt tttccagcag ttcatcatac acacgaactt ctgtttctgt tgctcttcct
    21061 agatctgcat ttagatggaa aagcagtgaa tcttcataaa tcgaacggtc caatacatta
    21121 ttgtcatctt tcattgcttg tttgatgctt gcaaaacgtt tattcaaaaa ataaatttgc
    21181 agcaaaaaag catactgttc agggtttgca taaaataagg gcaagacttc attatcatca
    21241 atcgattcat aaaatgcctg actatcaaag tgttcagcca tcatttctgt taaacttgat
    21301 tttccggcac caattgtacc tgctaaaacg atcacgctca tttacctcca gctcacttca
    21361 tcactaaata ttgtgtttaa ctccaacctt tttaaatata cccccaatat ctagtagtgt
    21421 caattgtatt tttttctagt caaagaagaa aaaaagtcaa aaaattctta agaaaaaagt
    21481 gttttttctt tagaaaacgt attcttagtt ttacttctga gatggtttta gtaaaataaa
    21541 ggaggaatgt tttttgagac ccttagtgga caaaaaaatt cgtgaagcag cgcaactcgt
    21601 tttgggcgct ttattttttg cttaaaaatc acttttacgt gagaaggagt tagattatgg
    21661 caaactatgg ccaaggagaa tcaagcggaa agatcatatt gatgggcgag cacgcggttg
    21721 tttatggaga accagcgatt gcctttcctt tctatgcaac aaaagtcacc gcattccttg
    21781 aagagctgga tgcaatggac gatcaactgg tttcttccta ctattcagga aatttagccg
    21841 aagctcctca tgcattaaaa aatatcaaaa aattattcat tcacttaaaa aaacagcatg
    21901 acatccaaaa aaacttgcaa ctgaccattg aaagcacgat tcctgctgaa cgtggaatgg
    21961 gatcaagcgc tgcagtcgcc acagcagtca ctcgtgcttt ttatgattac ttagcatttc
    22021 ctttgtctcg tgaaatacta ttagaaaatg tccagctttc ggaaaaaatc gcccatggta
    22081 atcctagtgg aatcgatgca gccgctacta gcagcttgca gccgatttat tttacaaaag
    22141 ggcatccttt cgactacttt tctttgaaca tcgatgcttt tttgattgtc gctgatacag
    22201 gaatcaaagg acaaacaaga gaagccgtca aagatgtcgc tcacctcttt gaaacccagc
    22261 ctcatgaaac tggacaaatg atccaaaaat taggatactt gacgaagcaa gcaaaacaag
    22321 caatcattga aaattcacca gaaacgttgg cacagacaat ggatgaatca caatcacttc
    22381 tggaaaagct gacaatcagc aatgattttc ttaatctatt gatccaaaca gcaaaagata
    22441 ccggggcatt gggtgccaaa ttaactggcg gtggacgcgg tggctgtatg attgcattag
    22501 cacaaacaaa aacaaaagcc caagaaatca gtcaagcact tgaagatgcg ggtgctgctg
    22561 aaacttggat acaaggatta ggagtacata cctatgttta aaggcaaagc acgcgcatat
    22621 accaacattg ctctaatcaa atattggggt aagaaaaatg aagaactcat ccttccaatg
    22681 aacaacagcc tttcgttaac attggatgca ttttatacag agacagaagt catcttttct
    22741 gatagctata tggtagatga attttatcta gatggcacct tgcaagacga aaaagcaaca
    22801 aaaaaagtca gtcagtttct tgaccttttt cgtaaagaag ccggcctctc actaaaagct
    22861 tcagtaatca gtcagaattt cgttcctacc gcagctggat tagcctcctc tgccagcggg
    22921 ctagctgctt tagcaggcgc ttgcaatact gctcttaagc ttggattaga tgatctctct
    22981 ctttcaagat ttgctcgacg cgggtctggt tcagcttgcc gaagtatttt cggtggtttc
    23041 gtcgaatggg aaaaaggcca tgacgactta agttcttacg ctaagccagt cccttccgat
    23101 tctttcgaag acgatttagc aatggttttc gttttgatca acgaccagaa aaaagaagtg
    23161 tccagcagaa atgggatgcg tcggacagtc gaaacatcca gtttttatca aggctggtta
    23221 gattccgttg aaggggatct atatcaattg aaacaagcaa tcaaaacaaa agatttccaa
    23281 cttctcggag aaacgatgga aagaaacgga ctaaaaatgc atggaacgac tttagctgcc
    23341 cagcctccat tcacttactg gtctcctgac agcttaaaag cgatggatgc ggtgcgacaa
    23401 ttgagaaagc aaggcattcc atgctacttt acgatggatg ctggccctaa tgtcaaagtt
    23461 ttagtggaaa acagccattt atctgaggta caagaaacat ttaccaaatt atttagcaaa
    23521 gaacaagtaa ttactgcaca tgctggtcca ggaattgcga ttattgaata aagggagatt
    23581 tttatgattg aagtatctgc accaggcaaa ctctatattg ccggagaata tgcagttgtt
    23641 gaaacaggcc atccagcagt tatcgctgca gtcgatcaat tcgtgacagt cactgtagaa
    23701 tctgcacgaa aagtcggaag tatccaatct gcacaatata gtgggatgcc tgtacgttgg
    23761 acaagacgca acggagaatt agttttagat attcgggaaa atccttttca ttatatcctt
    23821 gctgctattc gcttgactga aaagtatgca caagaaaaaa acattctttt atccttttat
    23881 gatctgaaag taacaagtga gttagacagt tcaaatggcc ggaaatatgg attgggttct
    23941 agcggtgccg tgaccgttgc aacggtcaaa gccttgaatg ttttttacgc gttgaattta
    24001 tctcagttgg agattttcaa gattgcagca ctagccaatt tagcagttca agataatggt
    24061 tcctgcggcg acatcgctgc tagctgttat ggtggctgga tcgctttctc aaccttcgac
    24121 catccttggc tccaagaaca agaaactcag cattctatca gtgagttact tgccctggat
    24181 tggccaggtc tatccattga gccattgatt gctcctgaag atttacgttt attgattggt
    24241 tggacgggta gccctgcctc tacttctgat ttggtcgatc aagttcaccg ttcgagagaa
    24301 gataaaatgg tggcttatca gcttttctta aaaaatagta cagaatgtgt caacgaaatg
    24361 atcaaagggt ttaaagaaaa taatgtaacg ttgatccaac agatgattcg aaaaaaccga
    24421 caattactgc atgatttatc tgcaatcact ggggtcgtca tcgaaacgcc tgctttgaac
    24481 aaattgtgta atttagctga acagtatgaa ggagccgcaa aatcttctgg tgcaggtggg
    24541 ggcgattgcg gaatcgtaat tgttgaccag aaatctggca ttcttccttt aatgagtgca
    24601 tgggaaaaag cagaaatcac tccactgccg ttacatgtct atagcgatca aagaaaggaa
    24661 aaccgatgaa tcgaaaagac gaacatgttt cactagctaa agcctttcat gataaacaaa
    24721 aaaacgaatt tgacttcgta cggatcatcc ataacccttt gccgcaaata gcagtttctg
    24781 atgtcgatct aagtacacag gcagttgggt ttacactaag cagcccattt tatatcaatg
    24841 cgatgactgg cggaagcgaa aaaacaaaaa aaatcaacca agatcttgca atcgtcgcaa
    24901 gagaagcaga tttgatgatt gccactggtt cagtcagcgc tgccttgaaa gtcccttctt
    24961 tagccgatac ttatacgatt atgcgacaag aatatcctca cgggaaaatc attgccaaca
    25021 tcggtgcagg gacttctgtc gaaagagcca aagaagctat ccgattgttt cacgcagatg
    25081 ccttacagat tcatttgaat gcacctcaag agttggttat gcctgaggga gaccgtgatt
    25141 ttaccaattg gaaagtactt attcaagaaa ctcaaacagc cattgacgtc cccctcatcg
    25201 taaaagaagt cggttttggc atgacaagag aaacgctcaa tgatctagct gctttaggag
    25261 ttcatacagt tgatatcagc ggtcgaagcg gaacaagctt cacccagatc gaaaacgccc
    25321 gtcgctccaa gcgggaactg agttatttag ctgactgggg gcaatcgaca gtctcttcat
    25381 tgcttgaagc aaatgaagca gacacttcta tggaaatcct agcctccgga ggtatccgca
    25441 atgcctatga catatttaaa gccctttgtc tcggcgcaaa tgccgtgggt acttcaggaa
    25501 ccgtactgac gcatctgatg aatcacggcg tagaagaaac tattatactg atgaagcaat
    25561 ggcaagaaga gcttcgtctg ctttatacta tggtcggcgc aacgaatacc gcggccctac
    25621 accaacaatc tctgatcttt tccggaccag tgaaggattg gtgcgaagca agagggatcg
    25681 acttagtgaa gtatggaaga cgcacagaaa agagcgtggg acaaaagtaa agaacacttt
    25741 tgtctcacgc tctaaacacg tacaaacggc ggaagtaaaa gcaactcctt cggaaataag
    25801 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt ccttcttatt tcttggagtt
    25861 aaatgcttct gtcccagcct cttttttcga ataaatgatg tttaatcagc tgtccttcgg
    25921 tattcgttca atccgctgat ttcacaacct ctccactaaa cagtgtacta aagctctttt
    25981 aaggttgttt tccgatatag gcaagtatgc ctccgtccac atacaagaca tggccgttaa
    26041 cgaaatcaga tgcgctagaa gccaagaaga cagatggtcc agccaaatcg attggctcgc
    26101 cccagcgtgc tgctggcgta cgtccaacga taaattgatc gaatggatga cgttcaccgt
    26161 tttcttgtgt ttcgcgtaat ggagcagttt gcggtgtagc aatataacct ggtccaattc
    26221 cattacattg gatattgtat tgaccgtatt cagaagcgat gtttttcgtc agcattttta
    26281 atccgccttt tgccgcagca taagcactga ccgtttcgcg tcctaattca ctcatcatgg
    26341 agcagatatt gatgatttta ccgccgcctt tttcgatcat atccggaata actgcttttg
    26401 aaacgatgaa tggggcattt aaatccacgt cgattacttg gcggaagtct gccgcatcca
    26461 tttcgatcat cggtgtccgt ttgatgatcc ctgcattgtt gactagaata tcaactgaac
    26521 ctacatcttc tttgatctgt tgaaccatcg cttgaaccgc ttcttcatcg gtcacatcgc
    26581 agacataacc ttttgcatcg atgcctgctt ctttataatg cgctatacct tcatcaactg
    26641 attcttgatt caagttatta aatacgattt ttgctcctgc tgtagctaaa gagcgtgcaa
    26701 tctcaaatcc aataccatag acagcaccgg tgactaatgc tacttttcca tctaaacgaa
    26761 acatatccat actaaattcc atcattttct ctcccttatg cttattttaa ttgatccatt
    26821 tgcaccatat ccatgtctgt ataggtgata ttttctccgc acattgccca gatgaatgag
    26881 taattgcttg tacctacacc tgaatggatc gaccagcttg gcgaaataat ggcttgttca
    26941 ttgtctacaa ctaaatgttt tgtttcatct ggtttgccca tcatgtggaa cacacgtgta
    27001 tcttcattgg cataatcaaa gtagacataa gcttccatac gacgttcatg tgtatgacaa
    27061 ggcatcgtat tccaagcgct tcctggttct aaaatcgtat aacccatttg taattgacag
    27121 ctttcgcata catttggatg gatgtattgg tagattttac gttcattcag tgtcaggcct
    27181 tcaccagtct ccattggttt tacttggtcg atactgattt ttacatttgg atatttatga
    27241 tgagctggaa cagaactaat atagaattta gctgggtttt cagcatcttg agaagaaaat
    27301 acaacatgtc gtgtttcttt tccgatatag tatccgtctt gttttttcat ttcttctttt
    27361 tgcccatcga tctcgatgaa tcctggtcca ccgatattga tcacacctag ttcacgacgc
    27421 tctaagaagt aatcaacacc tagttctttg tctaatgtga tttctaattc tttttcagta
    27481 ggtgtcactc caccaaagat cattcgatcg ttgtgcgtat aagtcaaact gattttccct
    27541 ggttcaaata ccttttctac taaaaattct tttctcaatt gttccgttga ataatgacgg
    27601 atatcttctg gactgtgtgt ataacgtgtt tccatttctt gcattttctt ctactccttc
    27661 tatcgaatga tttttccaga gccgcatgct aaaaattgtg cgacttcttc ttgactaaat
    27721 tggttgcagt ccccatggat cgtatgcttc aaggcagaag ctgccgtagc aaaatcaatg
    27781 atttcttgcg gagacatatt ggataaaatc ccatggagca cgccacctgc aaaagcatct
    27841 ccgccaccta ctcgatccac gattggggtg attctatgga cttgggattc cacatattca
    27901 tggttcatcc acaatgtccc cttcaattca ttttcacttg ctgaatgaac ggtacgcttg
    27961 gtcgaataaa acacctgaat attcgggaac ttttcctgca ttttttgata ataataaatt
    28021 agaggttgtt cttctgcttc tggcgctttc tcgataccta acaaataaag agcatccatt
    28081 tttccagctg agcagatatc tactaaaggt aaaagctgat ggattacttc gctggcttct
    28141 tgctgtgtcc ataattttcc acgatagttg atatcgaagc tgatcaggca gccagctttt
    28201 tttgcagctt ctatcacctg tttcgtcatt ttttgccatt ttttagacaa agctggggtg
    28261 attcctgaaa catgaaatat atctattcct tggaataact cctctagcga ccattccaaa
    28321 tgatccattt cagcaaaact tgatccggca cgatcataga tcactgaggc ggcgcgttcg
    28381 ccactaccag tttccatata ataagtacct agtcttgatc caccagataa tacctgtttc
    28441 gtatctactc cataacattg caagtgcttt ttgactgctt ttcccaatgc gttatcaggt
    28501 acttttgtag caaaaatcgc ttcatgtccg taattagcta aagaaattgc tacgtttgct
    28561 tcccctccgc cataatgggc agcgaatcgt tcacttgaat cgatacgggt tccctgctct
    28621 gtggacaatc gcaacatgat ttctccgagt gtcactactt ttcccataag cagctacctc
    28681 ttgatttctt tcatttttgc tgcatactgt tgcgcgactt ccgttacttt gtcaaaatct
    28741 cctgtagctg cgggtgctaa aaggttccca cctactccga ctgttacgac accagcatcg
    28801 aaccactctt tcatattgtc cagactcaca ccgccagttg gcatgatatt gacttgaggc
    28861 atcggcgcct tgaaggcaga aatgatactt ggtccataag cgcttccagg aaatagtttg
    28921 acgatatcta ccccgctttt cagcgctgtt ttgatctcgg taatagtcat acatcctggt
    28981 aagtaaggaa tttgatacag attgcacatc tctgccgttt cttgatcaaa ggaagggcta
    29041 acaatatact ctgcgccagc taagatagcc aagcgagctg tgacagcgtc taatactgtg
    29101 cctgcgccaa tcactatttc tggttggtct ttgtagaatg acaaaagttc tttgattact
    29161 tgatctgctt gagggactgt aaaagtcaac tcaataccag ttagaccacc tttgatgatt
    29221 gcgtggcaag cattcaaagc ttcttctttg ctttttccgc gtaccaccgc aatcactccg
    29281 gcgtttttca atcgagataa aatatcaatt tttttcatct tctccaccta tttcctattt
    29341 agaaatttta ttttataact tggaaatcta tacccatatt atacggcaga aaagaaaaat
    29401 tgcaaacgct tttttcatta tttttgaatt ctttgctcga tttcgctttt tgtttgcttg
    29461 agggcactga ttatttgttc ttttgtctca tctgttaaac gatatttcgg catacttaca
    29521 ctaaatgcac caatgatttg ttcgtttttc acgattgagg ctcctataca aaaaatatca
    29581 cgttcgattt cttcatcgtc aaatgcgatg tgggtttctt ttaccgtttt gatttccttt
    29641 ttcaaccgta atgcattggt gatcgtatat tctgtgtaag ggatcaacgg atgcttttca
    29701 agataatcag tgtactgctc ttcactgaat tctgctaata cggctttgcc catcgcagaa
    29761 ttgtataatg gtcgggtgat tccgattttt gaagacatgc ggatcgtttg atgttttgga
    29821 tctagtttgt tcacgtacaa gatctcattg ttgtccaata cccctaagtg gatagtctca
    29881 tcgatctttt gctgcagctg ttctaaaaac ggtaaagtca actctaccaa atcgatttgt
    29941 tcaatgtttt tattcgcgta gcgaataagc tttgcaccta aacgatagtt tttctctgaa
    30001 tttttattta cgtagccgat catcaacaaa gtatctagta ttttcaagac tgtagaagag
    30061 gtcattccca caccctttgc gatcgtctgc aaagagacat cttggttttc cgctaaatag
    30121 tctagtattt tagaggcttt gatcaataca gttccataag gctttttatt ttccatataa
    30181 tattctccta tttctttata agaaaaatta tagcggaaat accatcaatt taaaagagat
    30241 atccagatat aaactaccat ttctctttac aggcaattta atgttgaaga tccgcttcgt
    30301 ttttacatca aactttgata taaagcaatg atttcttcta ctgtcgtttc tcttggattt
    30361 cctggtgtgc atacatctcg caaagcatct tctgcaattg ctggaatatc tgcttctttt
    30421 acacctaatt cagaaatggt ttctgggatt ccaaccgctt tgctcaaccg atcgatctca
    30481 ccacaagccg cttctcttac atcttctaaa gacatttcag ctgctccttt aatccctaga
    30541 acaagagcga tctcacggta cttttctcca gtatattctt tgttatattt catgacagtt
    30601 ggcaaaagtg ctgcacacgc tacgccatga ggaatgttgt accatgcact taacgggtga
    30661 gccatcccgt gtaccagtcc taaaccgaca tttgagaagc ccattcccgc aatatactgt
    30721 cctaatgcca ttttttctcg cccattttga tcgccatcta cagaagcttc caaagaatgg
    30781 ccgatgattt cgattgcttt taaatgcagc atatcagtca tttcccaagc gccttttgtg
    30841 ataaaccctt caatagcatg cgtcatagca tccatacctg ttgatgcagc caattttttc
    30901 ggcattccca tcatcatatc gctgtcgatg aacgcgacga ttgggatatc atgaggatca
    30961 acacaaacaa attttcggtg atttacttca tctgtgatga cgtagttgat cgttacttct
    31021 gctgctgttc cagaagtcgt tggtatagct aatataggta aacatggatt tttcgtatca
    31081 gcaacacctt ctaaactaat gacgtctgaa aactctggat tcgtcaaaat aatcccaatg
    31141 gcttttgccg tatcgattgg cgaacctcca ccgattgcga tcaaacagtc tgcctctgcc
    31201 tctttaacaa aagccactcc atcttttaca ttttggatcg ttggattagg tacgatcccg
    31261 tcgtataaag cataagcgat tgctgcttta tctagaagtt ctgttacttt tgttgctata
    31321 ccgtgctcga tcaatccttt gtcagtaatg acagctgctt tgccgaatcc tcgctgctga
    31381 aattcaggga cgatcgactc aatcgctcct tttccaaaat acgatgtttc atttaaaatc
    31441 attcgattac tcattttcta ttcctcctaa tttgtattat ttttcacaat aaaaacgaaa
    31501 gaaccccaag caaccggttg cccagttgct tgttgctaca gttcaaagac tggcatcaat
    31561 tcccaactga ctggagaggc atctggattt gtctccatga tatcttccat gtacttccac
    31621 catttttgat tgatagatgt atttgccatt ttttcccata acttttcatc ttcgatcact
    31681 aagtaaccaa ataacaaact tgtactcgga tcaagataga tcgaatagtc gatgaccccg
    31741 tgttcacgaa gtgctgtacg catttctggc caaagttcat catgtctttt tttgtattct
    31801 tcttgttttt ctgggtaaac tttcatgcaa aaagcttttt tcaccatgtc agacacctgc
    31861 tttcgtatga acttcctcca aataacctgc ttttggagtg acaccaaacg catctgctaa
    31921 atgccacagg ttttcgtccg tgattgtttg caagattggt ccttgcgctt tgacgtacgt
    31981 atagacttct gctgcctttt ctgctgtctc gatcaaacca aagacttcat ccatgtcttt
    32041 tcctgacccg tagattccat gttgtggcca taaaaccaat cgagtttctt tcattttttc
    32101 agctgttgct tccccaatct catttgttcc cggaaccatc cacggaatga tggaaattcc
    32161 ttctggaaaa acaactaagc attccgtaca catttgccat agcgtacgac taaaggaacg
    32221 ttcgtctaat tcatgcgtaa aactcatcgc taatagatga ctagcatggc aatgcataac
    32281 gatccgatta tctgggtcta cttctaaacg ggcgatatga ctcatgaaat gacttggcaa
    32341 ctcgcttgta ggaaggcatt cgttttccag cccccacaaa agttccagtg ttttgccatc
    32401 ttcagcaact cgaacgattc caagattctc ggcaggttcg ctcgctacat ttttaaaata
    32461 tcttcctgaa ccagttacaa taaaatagca tcctgctaaa cggctggcat caaaaatcat
    32521 cgagatgcgg cggatcactt tttcaggatc caagaacggt gtgatctctt cttcttttag
    32581 taaataagaa atattcccgc catttctttc atcccatccc aagcgataaa gattcgacgt
    32641 cgtttcgatc atttctttca cataaggcgc ttgtaaaata tctatttttt tcaatatagc
    32701 cacactccta ttttcttaat tccaacacgt ctttttgata ttcctggact tcttccagcc
    32761 aatccaaacc aactggaact tgatttttgt aacagaaata attccataca tcggcatacg
    32821 ggaaatcttt caattcttcc gtataagcca ttcttgaagt aaagtcaaaa gccagctcca
    32881 tttctttcaa tcgctcaact ggttccagca tagctttcag cagtgctttt tgtgtatttc
    32941 tcgtaccgat gacccaagca gcgatacggt tgatcgttgc atcaaagaaa tccagaccga
    33001 tatttgtttt ttccaacaat tcgttacgga ctaattcttt agcgatttcc tgcaattcat
    33061 catccatcaa aaccacatgg tctgaatccc aacgcactgg acgggaaaca tgcaacatga
    33121 ttcctttgct gaataaagct aatgaagata atttattgga aatcacttct gttggatgaa
    33181 aatgtccagc atctagacaa acaagtttgt cacgagtcaa cccgtaaccc atataaaatt
    33241 catgggaacc gactgtataa gcttcagcgc ctaaaccaaa tagtttgctt tctactgcat
    33301 cttgtacata ccgttcatct aactcttctg taaaaatttc atcgagtgat tccattaaac
    33361 gttcccgtgg ggtaaaacga tcaatcggat tatctttcat cccatcaggt acccaaaagt
    33421 tattgatact tgtttgccca gtttctttcc caaagtactc cgcgattttg cggcttcttt
    33481 tcccgtgttc gatccagtat tcacgaactt ctttatctgg atgagccaat gtaaagccgt
    33541 ctttcatcat tggatgcgaa aagaaagtcg gattgaagtc aagaccgatt tcttcttttt
    33601 ttgcccactc tacccatttt tcaaaatgtt tcggctcaat ttcatttaga tcgacagatt
    33661 cttctgtatc caaatagata gcatgaaggt tcaatttgtg tttgcccggt atcaacgaat
    33721 aagccttctc cagatcttgt ctaagctctt caggtgttcg tgcggctcct ggaaaattcc
    33781 ctgtcgccat gattccaccg gtcaattcgc catccggtgt caagaaacct ttgacatcat
    33841 ctccttgcca gcaatgcata gaaattttga tgtcctgtat cttttctagt acagcatctg
    33901 tatctacacc gatagttgca tatttttctt ttgcttgttc gtaatttctt tcaatcgtgt
    33961 tcatacgatt actcctttga ttttattcac gtaccattcc atcgtgtttt gcttattttc
    34021 atctgggtaa aaagtttcac atggaaacga ctgtttgatt acatttcttg cttgttctat
    34081 attcgaaaac tgacctgtac tgagcatttg catggcaaca ttcccgattg ctgtggcttc
    34141 actaggtcct gcttctacaa tgattccggc ataatctgct gtcaattgat tcagtagacg
    34201 attattcgat ccgcccccta cgatgtgcag cttttccaat ttcgttccag ttaagtgttc
    34261 tactttgatc agctcttgtg cgtaacaaag agcaagatta tcatacacac aacgagcgat
    34321 agctcccgat gagacaggga ctggctgctc tgtttcaaaa cagtattgct gaatctcttc
    34381 tatcatattt gggggattca aaaatcgagg gtcattgatt tccatcactt gctgcagcgg
    34441 tttttctttt gcagcaagct ctgccaattc cgcataactg tactcgtact cttgcattcg
    34501 ggcaacttcc tggatcaacc acatccccat gatgtttttc aaaaaacgca ccgtgttatg
    34561 ggcaccccat tcattcgtat aattttcaga aaaagcattg cgagaaacat tggcgatctt
    34621 tgtttcaatc cctaaaagcg accatgtccc actgctgata taaccccaac gttctccttg
    34681 acctggcgtt cctaatacag ctgacgccgt atcatgactt gctactgtga taaaggtcac
    34741 atctggcaaa tcatattttg aaaattgttc tttttgcaga tctcctagta tcgttcccgg
    34801 ctctgtcaat ggtggaaata attccttcga gacaccgatt ttttcaagaa gttctatttc
    34861 ccattctttt gtccctgcat tcagcagctg catagtagat gcattagttt tctccgtcac
    34921 catgttacca gtaaagacat accctaaata atcaggtatc agtaacaatt tatgtgcttt
    34981 tgccaaattt tctttttctt cagcaaacaa ttggaacaag gtattgaaag gctggatctg
    35041 aatccctgtc tgctcataca acttatctag agaatagatt tcagaaaact gttctactgc
    35101 tccattggta cgcttgtccc gataagcaat cggatcattt atccgatccc cttgttcatc
    35161 aatcagacag taatcgactg cccatgtatc gatccccgca aaaaggcgat caatccctgt
    35221 ctgctttaat ttttcaaatc caatcaaaat ctctttgata agataatcga tcttccagcg
    35281 atcgtgaccg tctttgttcc gaaagccgtt tttgaaccgg tgtacttctt ccaagatcaa
    35341 agaacctttt gcatccctcg tacttttcat tagtcgtccg cttgatgcac cgatatcgat
    35401 ggcaacaaat gttggattca tttctccacc tcttttactt tattgttgat tagcacttta
    35461 ttaataaagc gttatcattt attgacaatg ctatcataat acagaatctt atattttgct
    35521 tcacactatt ttggtatata attgcattat cttgacaagt tgggagtgtt tacatggatt
    35581 actgggaaaa agccaaccac tcatggtcgg aagactcaaa aagagtgatc ttgacgccta
    35641 cacaaagaag caggaattta tttttttata tccaagaaat cggttacttc aaagcaccaa
    35701 tcccttacta tactgaacga gcacatttac cttcttactt gataaagttt actttgtctg
    35761 gcaaaggttc gcttacatat aatgataatc agcaacagct aaaagctgga gacttatttt
    35821 ttattgactg ccaagaatat cagcgttatc aaacgatcag cgaagaacca tgggaaatgg
    35881 attggatcca tttgaatggt cagaacgtcc aatcatttta tcaggagttc acaaaagacg
    35941 gccataacac cttccattca acaggagatc catcagaaaa caagatccac catttgatca
    36001 cagaattgat ccacatgcaa caaggcacga atgcccgtac cgatttccag tcctctgtag
    36061 ctatccacga gttgctgaat gaactgattt tgcaaaagta tcagctagat ttcgaagaag
    36121 aggatattcc cgcctacgtt tacaaactga agcaatactt agacgaacat ttcaaagaaa
    36181 cgattacact tgatcaatta gaacaacaat tcttattgaa taaatatcag ctgagcaaag
    36241 atttttccaa atttatcggt tctccaccaa tcgattattt gatcagcaaa aaactttctt
    36301 acgctaaaga ccttcttcgg tatacagaat ttacggttcg aatgatttcc gaagaaatag
    36361 ggattgagaa ttttgcttac tttagccgcc tgttcaaagc aaaaacagga ctatcgccga
    36421 gagactttcg taaattcagc tgattcatat aatatgtacg tcattttttc aaaatatttt
    36481 gaaatatata gataaaaata aaataaccgc agtttctgct tctatgtaaa caacagcagc
    36541 tactgcggtt attttttagt gatcattctc attgttcatc ttcaaaatgt tcataatgaa
    36601 ccaactgttt ctgtgtatca cgtttcaaaa attctttagg cgttatgccg aactctctct
    36661 tgaatgcttt cagaaaatgc ggataagaag aaaaaccact tttactatat acagcactag
    36721 agctgcggta ttctcgcaac aagtgcttgg catagatcag ctttttcttc attacatact
    36781 gatgaaacgt aaatccagtg tactttttaa attctcgtgt gatatggtat ttgctgacaa
    36841 aaaactgctt ttccatttcc tctaaagaaa gcggatgaga taaatttttt gagatatatt
    36901 gtaacatttg ttccacacgc tcattaggaa ctacagcatt ctgttcttgc gcactctctt
    36961 ctttttgtac aacttgattg agaaaaatca agtattctac cagagcttgt tcatatttta
    37021 tgtcttcacc gtaaattttt ttatcaggag gatggtcgat aaatgataat ttttgcttca
    37081 atacttcctg ttctatttgc aatacacgac tcctcttctc tcccattggt tgaaaacaag
    37141 ccgatagatt agaccatggc gtggaacgac tttctagaaa agaaggggtg acaaagatat
    37201 agacacgctc gaatacatct gaagattggc ggacgatacg atgcaaatca ttggaatgga
    37261 tgagcagcac acttcctgct ttgatcgtaa attgtcgtcc gttcaaataa aaagtcgctt
    37321 ctccctttaa tgtagcatgg atctcataga aatcatgatg atgatagatc gttttatttt
    37381 ggctgacgac atctttgaca tgatagattt catactgatc agaaaccatc gtattgactt
    37441 gttcaccgct gattttcttt tggagcatgt gccgcacctc cttttgttca cttatgcaca
    37501 attttattat accacgtttt ttgtaatcaa aaagagctgc ttttatatac tcttataaaa
    37561 aagaagagac gtggcatctt ctagccccgc cccctccatt ctaaatatta gttaaccaac
    37621 gtcttcttta cattattcaa gaagacttct tccataacga atcccatcat cttttgtttc
    37681 ttcaagaacc acatacaggt acggacgatt cttcatgatg atttctttga tttttgtgta
    37741 ctcgatcaca ccgtctccca aatttaccgg cacgattttt tcctgttcga cacgaaaatc
    37801 tttcaaatga accgcacata tcttttcacc aaaacgttca aaagcttctt ctaccaattg
    37861 gacttgttct tgataatcac tacttgtgat caaattggtc ggatctaaaa tgattcctag
    37921 atagtccgaa tctactgctt ggatcagctg ctctaccctt gctaaagaat acaccggatg
    37981 attcaaacct ggttctatcc ccaccatcat ttggtatttt tctccagctt ttaccaagcg
    38041 acggataacg gacacagctt cagcaaatgc ttcatctgtg aaattttctt ccgtatattg
    38101 gatttccggc aaaacacatc cagtttccga agcaaccatc gacgcaccaa aggaagaagc
    38161 atagcgaaga tactgctcga actttgtcag caactcttgt cgtatcgtta aatctggatg
    38221 aatcatattg atatagcaac ttaaaattcc tacggtcact tcttgctttt ctaaaactcg
    38281 tttgacataa tttcccatcc cagaattgat ctcatttgcg ccagaaggta tttcaggaaa
    38341 cgaaagacct aaggcgagct gcagtgtatg aatcccttgt tcttttgttt tttctgcaag
    38401 atcttctatc gtttggactt cggaaagatc atgtcctcta attgtaatgt tcaacataac
    38461 tattctccta actcagcata ttgggtagcc acatgacgat ttgcgggaag taagtaatca
    38521 aaaacaagac gactaagata accccataaa aaggaagaag cggtttcagc attgcctctg
    38581 cttttacgcc tcctacactt gcaccgacat atagtcctgt acctactggc ggcgtgatgg
    38641 agcctacaca cagattcatg attgaaaaca acccgtaatg tacaggatca acccctacac
    38701 ttgtgacgat tggtaaaaat attggtgtaa aaatcaagat agcaggcgcc acatccataa
    38761 acatacccac cagtaataac acaagattga ctaataacag aataataaat ttattatccg
    38821 ttacgcccat aatcaacgaa ctaattgcct gaggaatacc ggtaaacgcc atggcgaaag
    38881 acatcatgga agaagtagcc aataagaaca tgatcgtccc tgacattgct actgattgaa
    38941 acaacatatt aggtatatct tttagtttta ccgttttata gtaaaacata gcaatcaaca
    39001 gggagtaaat gacagctatc gctgaagctt ctactgctgt aaatatcccc gtcaagatac
    39061 caccgataat gatgacaatc aatagaaggc taggaatcgc ctcttttatt atttttccag
    39121 catgagccat ctgtcctttt tcgacaacag ggtatttatt ctttttagca acgatgaatg
    39181 ctactagcat gattcctagc gcccacaaag cgcctacgag ataacctccc ataaacaaag
    39241 cagagatcga agcaccacca gcaacaagtg aatacatgat aaaagcagta cttggaggaa
    39301 tgaccatacc tgtcggagct gacgcgatat tgaccgctgt cgcaaacttc cggtcatatc
    39361 cttcttccac ttgctgaggg atcaacactc cgccaatcgc ggtagaagcg gcaatggccg
    39421 agctagagat ggaaccaaat aaagcattcc ctaacacatt cgtatgagcc aaagcccccg
    39481 gcacgcgccc gacgaacaac atcgcaaaat ccactagttt cttagctatc cctccattat
    39541 tcatgataat gcccgagaga acgaaaaacg gaatagcgat cagcgaaaaa ctattgatac
    39601 ttcccaccat tttttgagct gaactaaaag cagaaacatc aaacggtata actaacaata
    39661 aagtcatcat cgatgagaat gcaatactta tagcaatcgg catccctaat acaagcaaca
    39721 caacaaatac aaaaaataac actaatccgg cttctattgc cattttattc ctccattttt
    39781 atattttttc ttgaattgct ggcttgttgc ttaatgtatc gattagattg atcaggctat
    39841 agataatgat gagtaccccc gatacaggta ctgacagata aacatatccc ataggaatac
    39901 ttaaagaagg cgagatctgc cccattgtca atgtgacagc attccatccg ccatacacca
    39961 tgatcatcgc agcaaatacc agaaataaac tttgaacaac aacttctaaa attttacttt
    40021 ttttgccagt taatttttca cttaaaaccg ttacggctaa atgtgctttt tgtcccacaa
    40081 catacgcgga agacaacatg gcaaaccaga caagaccgaa tcgaacaagc tcttccgtga
    40141 ccgtatttgg atttcctaaa accgttctgg aaaatacttg ccataaaaca acagcgacca
    40201 tcccaacaag caaaacagct cccaggatct ctaatacctt gtttaaaatc attttcatca
    40261 cagctacgct ccttgctctt ggatcttttg ataaaggtct ttgtaggtag gatcgttttt
    40321 gaatatatca tgaagtgggg caactgtctc tcgaaacggt gtattatcta catcattgaa
    40381 ttggactcca tgttcttctt gtgctaaagc tttttcttct tcaattgctt ttgcaaaagc
    40441 ttcaatttca aaaaccgttg attctttcgc tgcttcttca atggcttttt cctgctcatc
    40501 agttagctgt tctttgatgg catcattcat gatgatgata tctggcacac gtgtatgctc
    40561 atcataggaa tagtatttag ccacaccacc atgtccagct gtatacaaga caaattcatt
    40621 gttttctgat ccgttgatca aattggattg aagagacgtg tacacttcat cacttcccat
    40681 aggtacgggc gaaccgccta aaagctctac cattttgata gcggtttcgc tttgcatcac
    40741 acggatcttc tttcccttca aatcttccgg cgtatggatc ggaccgtcta ccatataaaa
    40801 gctgcgctga ccagaatcat aataggtcaa tccggtaaat cctaaatttt tggtcgattg
    40861 gtagattggc tccattattt cttcattatc catcacctta aagaaatgtt cttcatcatt
    40921 gaatagataa ggaactgaaa aaatcgaata atctttagag aaactttcca acgctgaggc
    40981 acttactttt gtgaaatcta ctgctcctga ctgtgtcaat tcgatcagtt cacgttctcc
    41041 acccagttgt ccatctggga aatactggat ctgtacggaa ccatctgttt tttcttttac
    41101 taaacgccca aattcattca ttgcatcaat tactggctgg ctgttgcttg cataagcaaa
    41161 gcgcaaggta actatctcat ttacatctgc atctgcgaca gttgccacac tagaattatt
    41221 tccgcagccg cttagaccta gcagacctag gctaagcgaa cttgctgcaa acagcagtga
    41281 tgtttttttc atatgggttt cctttctttt aacaataaga gacgagcaat tctgtcaaac
    41341 aaagcaccgt taacgactgt ccataaggca ttgctgtgat tccgatattc cgataaaaat
    41401 ctaaactgtc ccccattcct gtcccgattg aaacgttttg aacttctcct ttttcatcga
    41461 tttgttcaag caatccttgg atagcacgat aagcgacttc tttatattct tgtggtaaat
    41521 aacgtttatg gaccgctttc aaaataccgt aagcaaatcc tgcagttgca gaagattcta
    41581 cgtaagaaga aggatcatcc aacaacgtat gccataaacc gctttcagac tgataagttt
    41641 ttaatgcacg cacttgtgct tcaagtgttt cgatcaataa tttgcgcaat gcatcttctt
    41701 tcgataattc taagatttca atgatctctg gaatagcaat cgtcaaccag cagtttccac
    41761 gagcccaaaa tgcttcggca tagttatgat ttccttcaaa cgtccagccg tgataccaca
    41821 atcctgttcg tttatccatc aagtatttga tatggatcat aaactggtaa cgagcttctt
    41881 ctaaatattc tgggcgattc aacagtttcc cgatttttgc caaaggaagc actgtcatca
    41941 tcaatgtatc gtcccacaac tgattcttat tttccggtcc atatgtggca tgctgtaacc
    42001 cgtcttcatt tgtccgaggc atgtcattca tgacccattc tgcccattgt tctaaataag
    42061 gcaaatagcg tgaatctttc gtgtcttcat acaagtaagc catagtcaat agcggtgcca
    42121 ttgtattgac gtttttgggc ggagcgcctt cttccatacg atcctcaaac cattcattga
    42181 ccacttgata agcgcgagga ttttttgtca tctggtaatt tttataaata ccatatagac
    42241 cgacaccttg tggccaattc cacactgtcc agcttttgtc atctactttc agtccatcaa
    42301 agtccaacaa aaattcacca ttatcgtctt tgatcgttgt caggttttca acgagcaggt
    42361 caattttttc tttcactaat tctttaggta caaaaatctc ttctttaata atcatcttta
    42421 cgccaccttt ttaaacgctt tcattttata tccttaatat agcagtaata ccgtccacta
    42481 taaatatcgc tttttgaaca aaaactgtct aaaattgacc taaatgttaa ataacaaaag
    42541 taactttcag ttacttggaa attaaaaaaa gaacttggag aaacaacgtt ttccaagttc
    42601 tttttcatct attttcttat cttttttcta cgctttcttt tgcttatccg ttgtaagcat
    42661 caatgatgat ctgtttcaat tcagagatca atggttcttt cggattagct gtcgtacatt
    42721 ggtcttcgta agctaattca gccatacggt ctacggttgt atctagtgtt tcttgtgtga
    42781 ctccttgagc ttttagactc atgttgatgc cgacagattt tcctaattca taaacagccg
    42841 ttgctaaagc ttcgactaat tcctctgttg tttcgccttt cagacctaag aattttgcga
    42901 tatctgcgta atctgtatct gcacggaaat agtcatattt agggaacatc gcatgttttt
    42961 gtggatcttt tgcattatag cggataatat gcggtaaaag aatcgcattt gtccgtccat
    43021 gagggatccc gtattccccg ccgattttat gtgcgactga gtgacaaata cctaggaatg
    43081 cgttagcaaa cgccatacca gccattgtag aagcgttgtg cattttttca cgtgattcca
    43141 tatcaggtgt ttttactgat ttttctaaat agtcaaagac taacttgatt gcttgtaggc
    43201 tcaatccacg tgtataatct gaagccatga ctgaaacata ggattcgatt gcatgtgtta
    43261 acacatccat cccggtatct gctgtgacag atgctggaac tgacatgacg aattgaggat
    43321 cgacgattgc gacatctggc gtcaatgcat aatctgctaa cggatatttc acatgtgttt
    43381 cactgtcagt gatgaccgca aatggcgtta cttcagaacc agtccctgat gttgtcggga
    43441 tacatacgaa ttgtgttttt tctggtttgt cgatcttata tgtccgttta cggatatcca
    43501 agaatttttg ttttgcacca aagaaagatg tgtctgggtg ttcatagaac atccacattc
    43561 ccttagctgc gtccattgca gaaccgcctc caagagcgat tactgtatct ggcttgaagt
    43621 ctgccatcag tttcgttcct gcgtatacgg tatttgtcga tgggttcggt tcaacatctg
    43681 agaagacttc gatcttcaca tcatttttac gtcgtgccaa tacttcacga actgtatcgc
    43741 agtaaccgaa ttggaccata ccagggtcac aaacgatcat gacacgttca acgttttcca
    43801 ttttctctaa gtacaataga gagttctttt caaagaagat ttttggcggt aatttgaacc
    43861 attgcatgtt atttctccgt ttcgctacag ttttgacatt gattaagttt acagcagaga
    43921 cgttacgaga aacagagttc ttaccataag aaccgcatcc caaagtcaat gacggaatca
    43981 tttcgttata gatatcaccg atgccgcctt ctgcagatgg tgtattcact aaaatacgac
    44041 aagctttcat tcgaagaccg aatttgacat gcaactcttc gttttctgaa tggataactg
    44101 ctgtatgtcc taatcctcca agattcaaca taccttcaca caaatcaaac gcatggtccg
    44161 tgttgttagc tttgatcatg gcaagaactg gagaaagttt ttctcttgat agcggataat
    44221 ctgggcctac gccttccaat tcagcaatca gcattttcgt tccttttggt acactgattc
    44281 ctgccaattt tgcgatttcc atcgcagaat gtccaacgat tgatggattg accgcatatt
    44341 ttccttcgtt catcactgca tcttctaatt tttgcaattc atctggtttt acgatataaa
    44401 cttggtgtgc ttggaattct gcttttactg ctgcatagat ttctttatcc acgatcacag
    44461 cttgttcaga agcacaaatc attccattat caaatgtttt cgaaacgatc aaatcgttca
    44521 ctgcacgttt aatttttgct gttttttcaa tataagctgg tacgttacct ggacctacac
    44581 ctaaagctgg ttttcctgta gagtaagcag acttgaccat tccagctcca cctgtcgcta
    44641 agacgatcgc gatacctgga tggttcatta gcatagaagt cgcttcgatc gaaggatgtt
    44701 cgatccattg gatacaattt tcaggagctc cggcagcgat agcagcatca cgtaccactc
    44761 gtgctgcttc tgcagaagat ttttgggcac ttggatggaa agcaaaaacg attggatttc
    44821 ttgttttcaa cgcgatcatt gatttgaaaa tcgttgtgga agtcggattc gttgttggag
    44881 ttactccgca aaccacaccg actggttcag caatttcaat caaccctttt tggacatctt
    44941 cattgatcac accaactgtt ttatcatgtt tgatattatg ccaaatatat tcggacgcat
    45001 acatattttt gattgcttta tcttcgtaaa caccgcggcc tgtttcctct acagccatct
    45061 tcgctaatgg catatgttga tccaaagctg ccattgccat ttcatgcacg atatggtcta
    45121 ctttttcttg atcaaagctt tccatttctt tcaatgctgc atttgctttt tctgccaaat
    45181 catcaatcat tgcttgtacg tcaattgtct tcgttttttc tttttctatt actttagcca
    45241 tcattatcct cctaatgggt catggttatt tgttaaataa ttcacatgta tatcatacaa
    45301 tatttatttt tctatgtaaa cacttttttg tgattttttt cacagttatt tttaaatata
    45361 aaattgc
//
LOCUS       DBKOIJ010000005        44660 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000005 DBKOIJ010000000
VERSION     DBKOIJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 44660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.005"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(6..1046)
                     /locus_tag="ACV5NJ_000198"
     CDS             complement(6..1046)
                     /locus_tag="ACV5NJ_000198"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HHY6484330.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1323..3125)
                     /locus_tag="ACV5NJ_000199"
     CDS             complement(1323..3125)
                     /locus_tag="ACV5NJ_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HHY6484331.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3139..3891)
                     /locus_tag="ACV5NJ_000200"
     CDS             complement(3139..3891)
                     /locus_tag="ACV5NJ_000200"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484332.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3900..4262)
                     /locus_tag="ACV5NJ_000201"
     CDS             complement(3900..4262)
                     /locus_tag="ACV5NJ_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HHY6484333.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4438..5310)
                     /locus_tag="ACV5NJ_000202"
     CDS             complement(4438..5310)
                     /locus_tag="ACV5NJ_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HHY6484334.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5361..5849)
                     /locus_tag="ACV5NJ_000203"
     CDS             complement(5361..5849)
                     /locus_tag="ACV5NJ_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HHY6484335.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(5987..6778)
                     /locus_tag="ACV5NJ_000204"
     CDS             complement(5987..6778)
                     /locus_tag="ACV5NJ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HHY6484336.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(6833..7441)
                     /locus_tag="ACV5NJ_000205"
     CDS             complement(6833..7441)
                     /locus_tag="ACV5NJ_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HHY6484337.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7434..7751)
                     /locus_tag="ACV5NJ_000206"
     CDS             complement(7434..7751)
                     /locus_tag="ACV5NJ_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HHY6484338.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7907..8635)
                     /locus_tag="ACV5NJ_000207"
     CDS             complement(7907..8635)
                     /locus_tag="ACV5NJ_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HHY6484339.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8794..9675
                     /locus_tag="ACV5NJ_000208"
     CDS             8794..9675
                     /locus_tag="ACV5NJ_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHY6484340.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9912..10955)
                     /locus_tag="ACV5NJ_000209"
     CDS             complement(9912..10955)
                     /locus_tag="ACV5NJ_000209"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HHY6484341.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10958..11626)
                     /locus_tag="ACV5NJ_000210"
     CDS             complement(10958..11626)
                     /locus_tag="ACV5NJ_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHY6484342.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11768..14104
                     /locus_tag="ACV5NJ_000211"
     CDS             11768..14104
                     /locus_tag="ACV5NJ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHY6484343.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14219..14530)
                     /locus_tag="ACV5NJ_000212"
     CDS             complement(14219..14530)
                     /locus_tag="ACV5NJ_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484344.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14711..16186
                     /locus_tag="ACV5NJ_000213"
     CDS             14711..16186
                     /locus_tag="ACV5NJ_000213"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HHY6484345.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16233..16817
                     /locus_tag="ACV5NJ_000214"
     CDS             16233..16817
                     /locus_tag="ACV5NJ_000214"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HHY6484346.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16819..17238
                     /locus_tag="ACV5NJ_000215"
     CDS             16819..17238
                     /locus_tag="ACV5NJ_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HHY6484347.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17519..17803)
                     /locus_tag="ACV5NJ_000216"
     CDS             complement(17519..17803)
                     /locus_tag="ACV5NJ_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HHY6484348.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18155..18865)
                     /locus_tag="ACV5NJ_000217"
     CDS             complement(18155..18865)
                     /locus_tag="ACV5NJ_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HHY6484349.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18862..20334)
                     /locus_tag="ACV5NJ_000218"
     CDS             complement(18862..20334)
                     /locus_tag="ACV5NJ_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HHY6484350.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20472..20945
                     /locus_tag="ACV5NJ_000219"
     CDS             20472..20945
                     /locus_tag="ACV5NJ_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHY6484351.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21098..21649)
                     /locus_tag="ACV5NJ_000220"
     CDS             complement(21098..21649)
                     /locus_tag="ACV5NJ_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HHY6484352.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21754..22284
                     /locus_tag="ACV5NJ_000221"
     CDS             21754..22284
                     /locus_tag="ACV5NJ_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HHY6484353.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22767..23210)
                     /locus_tag="ACV5NJ_000222"
     CDS             complement(22767..23210)
                     /locus_tag="ACV5NJ_000222"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6484354.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23226..24386)
                     /locus_tag="ACV5NJ_000223"
     CDS             complement(23226..24386)
                     /locus_tag="ACV5NJ_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HHY6484355.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24893..26593
                     /locus_tag="ACV5NJ_000224"
     CDS             24893..26593
                     /locus_tag="ACV5NJ_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484356.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26695..27528)
                     /locus_tag="ACV5NJ_000225"
     CDS             complement(26695..27528)
                     /locus_tag="ACV5NJ_000225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HHY6484357.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27832..29997)
                     /locus_tag="ACV5NJ_000226"
     CDS             complement(27832..29997)
                     /locus_tag="ACV5NJ_000226"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HHY6484358.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30257..32551
                     /locus_tag="ACV5NJ_000227"
     CDS             30257..32551
                     /locus_tag="ACV5NJ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HHY6484359.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32544..33209
                     /gene="pgmB"
                     /locus_tag="ACV5NJ_000228"
     CDS             32544..33209
                     /gene="pgmB"
                     /locus_tag="ACV5NJ_000228"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HHY6484360.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33202..34209
                     /locus_tag="ACV5NJ_000229"
     CDS             33202..34209
                     /locus_tag="ACV5NJ_000229"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HHY6484361.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34231..35250
                     /locus_tag="ACV5NJ_000230"
     CDS             34231..35250
                     /locus_tag="ACV5NJ_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6484362.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35531..36898)
                     /gene="nhaC"
                     /locus_tag="ACV5NJ_000231"
     CDS             complement(35531..36898)
                     /gene="nhaC"
                     /locus_tag="ACV5NJ_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HHY6484363.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(37007..38404)
                     /gene="tyrP"
                     /locus_tag="ACV5NJ_000232"
     CDS             complement(37007..38404)
                     /gene="tyrP"
                     /locus_tag="ACV5NJ_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HHY6484364.1"
                     /translation="MGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIFAVLFFAGP
                     ISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVASTLGPLLG
                     NTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYIPVVIMFVL
                     GVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMSSVYMPRLK
                     DATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDCQILGLPEV
                     IGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYGVSRNVVLT
                     QSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKKHPEFERPY
                     RIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVIPLIINHFK
                     KDSWGIEVEKSLEEK"
     gene            complement(38671..40548)
                     /gene="tdc"
                     /locus_tag="ACV5NJ_000233"
     CDS             complement(38671..40548)
                     /gene="tdc"
                     /locus_tag="ACV5NJ_000233"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HHY6484365.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40839..42095)
                     /gene="tyrS"
                     /locus_tag="ACV5NJ_000234"
     CDS             complement(40839..42095)
                     /gene="tyrS"
                     /locus_tag="ACV5NJ_000234"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HHY6484366.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(42207..42412)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42675..43976
                     /locus_tag="ACV5NJ_000235"
     CDS             42675..43976
                     /locus_tag="ACV5NJ_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HHY6484367.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(44045..44644)
                     /locus_tag="ACV5NJ_000236"
     CDS             complement(44045..44644)
                     /locus_tag="ACV5NJ_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6484368.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        13594 a         9220 c         7905 g        13941 t
ORIGIN      
        1 ttattctatt tctttatatt tattttgtat tccgttacaa actcgcgtac ttggttaggt
       61 tccagagtga catctccaaa actttcaagt cgctccgctc ctggcagttc ttgagtctca
      121 aatgtgatac cgccatgatt tactaattta ttcccgcgca tttccggtcc atcttctcca
      181 aaatttgctg taaaaatcac gattcctgaa gcatctgttt ttacttggat cgatactttt
      241 tcatctgggc ttgtcaattc cgcagcattt agtaaaccag attgtttttt caacaaaaat
      301 ggatgatcga tgccgcccac tatcattgat tggcgaaaat cactggcaaa gacctcacct
      361 aattttttag gacgttggaa gtcaaatgct gtgccactta ctgaaactac ttctcctgta
      421 ggcgttgtat ctttattgag cgtagcaaat aatcggctat tcaaccaaag tatatgttcg
      481 tcgattgact gtgctggatc accggttaaa ttgaaataga catgatttgt aggattgaat
      541 actgttttgc ggtcactgat cccttggacc gtcactcgcc atgtatcatc gttcgtcagc
      601 gtataccgta cttctacatc taaagttcca ggaaatccgt tttccatatc tgggctagtc
      661 tgatagaaaa tgaccgatgc ttcgttttca ccattgatga ctgtataatg ccactgcttg
      721 gcttcaaagc taggtgcacc accatgtaaa caatgaccag tgtccgggtc tgtttttact
      781 tcgaacgttt catcatctaa tttgaatgtg ccattcgtaa tccgtccagc catacgtccg
      841 attgaagcac cgatataagg gtctttttct agatattctt ttgcttcatc aaaacctaag
      901 atcaactctc gtttttcatt atcgattatt gtttgaaaat gaacaacccg tgccccaaga
      961 tcactgacag acaaagatgt cccattttga ttcgtgatcg taatcaagtg atagttttcg
     1021 ccaaaatttt gttcattgat ttccatgttc cttgcaccct cttttctttc atttgcactg
     1081 attccagtat acatggaaac cgttttctta acagcttatt ccgtgagtaa cgagaaaaaa
     1141 tttactatat tctaataaaa gaccgtgaaa aaaaggttgt gacaaaaata ttgtcacaac
     1201 ctctttttcc gaacaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
     1261 cgaaaaatat gaggagctat ttttgcttgg ctgttcttat tacttgatgc aggacagctt
     1321 tttcacaatc tctttttcaa tacgattcca gtatataacc atgtcgtcag tacatagtag
     1381 atgaaataca atacgaagaa tatcgtaaac ggaacccaaa tatctttgta tggattttgc
     1441 atgaatagac caaacatttg taatccgaat aagacatgga ctacacctaa gattcctggt
     1501 gctaagaata acacgcctac ttctctacgg atcgattgtt taagcaaccc gcgtcgtgtt
     1561 cctatctttt caagcatttc ataacgagca atgtcactct tgcttcctga caagatcttg
     1621 aacatcaaac agctagcaag catggtcaag aaagctaaac ctaggaagaa tcccataaat
     1681 tcaaaaccag agaatagacc attatacact tcatacattt catatttctg gttgctgtac
     1741 tctacttttt gcaaagatgg attgttcatt ttattttgag caacaagtgt ttcgattggt
     1801 ttcaggtttg cttggaagtc ttttacctga actagctgta aagtggattg tggcaagttc
     1861 agttgatcaa attctgattg actgactacc ttgatttctt tttcttgctg ttccggcaat
     1921 tcatagcttc gaagttcctc ttgtacagtc agttcattca tttttttagc aggaactttt
     1981 tctttcgtga tcttcggcgg tgttccatct tcatgtttta tcatgatcaa tggttgagcc
     2041 gcaaattcac ttgcattata gtaaatcgtt cccgcatctt ctttttgagt ataagaagca
     2101 gaaagtgttg gtgaaagttc tttgattttt tgctgatctt ctgcccgagg attattcatc
     2161 actagatcat atccagaatt gttttctgtc aagtctgcaa tttcattttt aaaacctaat
     2221 cctaccgtca aagcgcccag tgccaaggca aacagcatag cgaccattga gagcatttgt
     2281 gtatattcac gaatacgaaa gtttaactgt gacaatgtga aattgttcaa ttttttcaat
     2341 gaaatctgtt ctgatttctt tagtaatgat aaaaagaaaa tgatcacaga atggaaaatg
     2401 aaataagttc ctaatacgat cgtaactaag gctaccccta taccaaagac ttgataggtc
     2461 cccacgtgtt tcatcatata gtaaccgcag cctagaaaag caattcccag tactacttca
     2521 aggaaaaaca agaatttatg ctgcttgatt cttgttggtg tctgatccgc acgaatcaat
     2581 gctaaaatcg gttttttgac aatcgaaaaa gcattgacaa cagcagctaa taaaaacaaa
     2641 acagcaaaaa acaaaacagt gatcaacaac ccgtgaatac taaaaggtgt aaaatgcgaa
     2701 atattgagat ctaactgatc cactaataat cggtggacga tacttgtcaa tcctacaccg
     2761 acagctgaac ccactaaagt agctgcgatc cctactaaga atgtttcaga aaaaatcatc
     2821 tgtgcgattt ttctgccttt tgctcccagc atcataaaca ttgcatagtc tttttgccgc
     2881 atcgtcatca aaaaggaatt tgcatataaa atataaacga atgtgatgat ccctagtaaa
     2941 acagaaccga atcggaaaat gataactgta gccgcaacga tcgtattact tttcaaaaaa
     3001 gcttcgttgc ttgccattga ttcaaacatg taaaaaatag cagatgccat aactaatcct
     3061 gagaataaca ccaagtagtc tcttaaacgg ctcttgatcc cagtaagtga taatttccaa
     3121 agcatagtca atctccccct actgttccaa gtttccaaga atagatagta tttctcgata
     3181 gaaggaatca cggctttgat ctccacgttt cacttcttga tggataccgc catccttgat
     3241 gaataaaata cgttggcaat aacttgcaga aaacggatca tgcgtcacaa gaagaatcga
     3301 tacatgatct tttgtattca attcgtccat cgtatctaaa agatcccggg cacttttgga
     3361 atccagcgca cctgtaggct catcccctaa tagaatcgtt ggttgtgtga tcaatgcacg
     3421 tgcggcggcg acccgttgtt tttgtccacc ggaaatctca gaaggatagc tttccaaaat
     3481 atgagagatg gataaacgtt ctgcaatttt ttggacacgt tgcttgattt cttttggtct
     3541 gacgccttgt aaagaaagag gaacagcgat attttcttgt gctgtcaagc tttctaataa
     3601 attgaaatct tggaaaataa agccaatctc attcgcacga aaatctgcta gttgatttcc
     3661 ttttaatgtg gtcacatctt tttgattgat tcggatatgc ccatctgttg gtttatctaa
     3721 tgtggataaa atattcaaaa gtgtcgactt accagaacca gaagcgccca tgatcccaat
     3781 aaattcgcct ttttctacat cgaagctgat gccgcttaat gcttgcgtct ttttctcatt
     3841 gttttttccg tatacttttg ttacattctg tacttcgact attttgttca tttctttccc
     3901 taccctttaa ttagtttttc cagtgctgtt ttcggcacgt cttttttttc gacttcttca
     3961 tacgaagtga cacctttatt tttgttccag gtgattttta aatatgctcc tttacgaagc
     4021 ggtcgagtca attgtccctt gaagtccagt tctttttcct taccctcttt cgtatagcca
     4081 ggcaggatat atctatagtt gactgtttct tgtccagagt ctgattttga aactgttttt
     4141 gtaccatctg tcgtaatttg tacatagtat tcttctccac ccataactac gtaatctgcg
     4201 attttcaccc ctgcaagtac cacaacgatg aaaacaagta gaccgatcag aagttttttc
     4261 actttttccc cttctctctt actttatata ctgattatag aaaaaagcga acaattctgc
     4321 cttcgaatca actatcattc ttctttcaat tttgtcattt tctttaaaac aactaaaaaa
     4381 agagttcaag acaataatcc gtcctaaact ccttcttcct atcttacaaa aagtatgtca
     4441 cttacggtct ttcatgtcgc ctgtattgat aacaggttgc gttcctttgt ctgtaatgat
     4501 tgagacaagt aaattattga tcaattggac tttccgatca tcggtgaaat tgatttcttg
     4561 tccatcttcg atttgctcta aggccatttg agtgattgtg acagctcctt caacaatggt
     4621 ttgtcgagca gacaaaatgg cttttgcttg ttgacgttgg agcatggcac tagcgatttc
     4681 tgttgcatag gctaaatggt tcaagcgtgt ttcgattact tcgacacctg ctactgctaa
     4741 acgctcttgc agctcttttt gcaactcgtc agacactgcc gtcgtgtttc ctcgaagcgt
     4801 caaatcattc tcattgaatg tatcatacgg gtattggcta gcgatatgcc gaatggctgt
     4861 ctcactttgg atttctacaa aatcttgata atacgctaca tcgaataatg cctttgctgt
     4921 atcaacgact ttaaagacta ctactgctga gatttcgatg gggtttccat ccaaatcatt
     4981 gacttttaaa acagaactat tgaaattccg aactttcaac gaaacagtca ttttttgtgc
     5041 caaaggtatc gtaaggaaaa agccattttc tcgaatcgta cctaaatatt gtccaaaaaa
     5101 caaaattact tttgcttgat taggactgac tacagttgcc gaacttaaca acaataacgt
     5161 gatcaaccaa agaaaaatac ttaaaaaaag tgcccaaact ttcgcttgcc acatacctaa
     5221 atagaagaaa aacaatccaa ctaaagctaa tacgaccaat ccgacaatcc ctgcataacc
     5281 attgatataa aatgctcttt tttctctcat gctacttcct gcgctccttt atccttcaat
     5341 aaatatctaa tgttactttt ttaatttttt tttcgaatac gcgccattac accttctagt
     5401 tctaactctt tgtcttcagt gatgtaccaa ccgcttgcct gtgtccctcc ggctgcctgc
     5461 ggtaaataat ccaattttgt tcgaacccag tgcttttctt ctgtaaaata gtcttctacc
     5521 aagacctcta gatccatccc tgctgaaaga ggcaaatctt cccgaacaga tttaaagata
     5581 aatcgttcct tcttcccatc atacttcaat attcctgtat cgataatcgg taaagaataa
     5641 taatccagct ctttcttcac cgtcaataaa tgtttgaaaa cttcatggta cacttgctct
     5701 aattggcgac cttctgaatt gttcgggtaa ttaaccatca ctttttcttt gtctggatca
     5761 tagaaggttt ggcttagttg agtgatataa gtatgtaatc tttccatttc tgtttttaac
     5821 ttttctcttt gaccttcttc taccttcata tactctttca ccctgttttc tttttagata
     5881 ttttctttct tattttacta tagttatgtg cgttaagcga tgaaaaacag caaagattgg
     5941 aaggatcttt gctgtttttt cttaaaatat ccagttgagt acaggatcaa tctgtttcat
     6001 tgtcctcttg atccgtcgta atattgatcg atgtgtcttt agcagtgatc tttagctttt
     6061 tctgtccttg ttctaacaaa gtatttgtta ttggctggta gtctttttca atataagatt
     6121 caccagacgt ttggatagta tagccactgt tttccatctc atgcaaagat aaattgccac
     6181 cgtttaggat gacttttcct tctttgctga tcaaactgtc ttctagatgg aagttgctgt
     6241 tttcacctgt gacgatcaag ttatccacat tggcctcatc taatcgaagt gagcctaatt
     6301 catctttcgc agttattgtt cctcctgtaa attcattgaa cgatacccag ccatcatgtg
     6361 tcttagtatt cacttttttg attgatactt cgttgatatc aattgcacta ttattccctt
     6421 cgattgtcaa ttcagataat tcatgatctg cagggacaac aatagtgatt tgtgcacttg
     6481 tgtcttctaa actaatgatt gcacctgttg attttttgac tgcattcaca gtcaacacgc
     6541 catttttctc ctttatttct ggcttttctg tcttatcata agtcgttttt atctcaaaat
     6601 ccaatccgcg tatgaccgaa actgtttgat tttctgcatg gatgacgatt ttgtttgcat
     6661 ccttgatttt ttttgtctca gtaaccatat cgatcaaccg aggaccatta tcccaagtca
     6721 gactcttttg agcgcctaat gtataagttg cacctgctag gaggccacca gtaagcatca
     6781 gaccaatacc ggtaatcaaa aaatgtttca atctcaattt atgtgtcctc cttcaaacgc
     6841 ccgctgaatg cagatctctt tttgtaataa aacgtcgtcc catccattta acaaagttca
     6901 tcatgacata aaaaagccct tttgttcctt tcagcaacag tggcgaaata aatactgcgc
     6961 ttcctgtggc gaacagaccg acaccgataa agaataatcc actcagaatc gattgtccaa
     7021 acacactgat tcctgagatg atcatgaata ggccaacacc taagatactg atacataaaa
     7081 catagatact aaaaatgagc gctagtaaag caaccaatag tcccccaata accaatataa
     7141 accccagtgc tagtggaatg aggacaggag aagcaaataa accgataatg attaaccaaa
     7201 tcaggcgaat ttgtcttttt ggatttttca ctaactcgtt ttctgcttgt tcttcttctg
     7261 aaaagtaact gatttttaat tttaaggcaa actgctttgg tgtaccatat tcatcaatga
     7321 tctgatctaa tgtttttcca gaatctaaaa aatattcctc ataatactgg atcaaatcaa
     7381 aacgatcgtc ttcaggaatt tcttgaagat gatccacaac ttcattcaaa tattcattca
     7441 tttgtgctct cctccaataa ccggtctaaa gaatctcgaa aaacaatcca ttcatcaata
     7501 atttcttcta aatgacgact tcccattggt gtcaacgagt aatatcgacg gtttctgcct
     7561 tcaaaaggtt catcataggt ctcaagatat ccttcttttt tcaatcttct caaaaccgga
     7621 tatagtgtag attcactgac agagagactt tcttgtacga tttttgttaa ggcataccca
     7681 tacatatctt ccttgcttaa tgctgctagc acagtaccat caagaagaac agttggaatc
     7741 tggatgtgca ttacgtcacc ttctattctc aagtgtaata ttttatttca atctatatta
     7801 tacaacatat aatatagttt caaaatcagt ttgtcaaata caaaaaaact gactaaaaga
     7861 tttgatcctt tttagtcagc aaaaaatggc ttctctttga tttttattat aacagtggca
     7921 taatccaatt tgtccacatc cagctcagtg gcatgataac gatcattgca ggtatcatgt
     7981 cagctactgg gaacattttc acgttgatca ttctaaaacc ggtagcgacc atcaagaatc
     8041 cgccgcaagc tttaaaatct aagatcatgt ctggtgtagt caatggataa ataaattttg
     8101 ccaataagaa caaaatataa aaaataatga attggggaat tgctatcaca gaaacgacat
     8161 accctaaatt acacgcaaaa atagctgctg tgaaaaaatc aaggacagat ttagaaatca
     8221 aaatcgtact gtctcctgtc atcccagaat ctaaacttcc atagatcccc gtaccacttg
     8281 cacaaaatag aacaatgacc gtcactaatg tgctgataaa ttcatcgtgg gtcataccaa
     8341 gcttttcact agggaagatt ttagaaatag gtacttgcat cagcgcagcc cccttattta
     8401 tcccattacc taaatgaaca atcaaaccaa tcgctgttcc tatcactaaa gcaaaaataa
     8461 cagccggcat atttttcatc ggcgcaatgg aatagattcc catccccatc gaacacacac
     8521 caaaaaccat cgtcagctgt gctttaaaat gttcacttaa tttatttcct actaatccgc
     8581 caattagtcc gccaaacaaa acggaaccta cattgatcaa aactccagtt ggcatcaaaa
     8641 aactcctctc tttttctacg aagcgaaaaa aatcagaaaa atttctgtaa ttattgcttt
     8701 cgctgcaaga aagattgtaa cagacagaga agattacacc aattcatttt ttgtgttaaa
     8761 gtattcttta ttggaataag taggaggaat atgatggata tccataaatt cgaagttttt
     8821 cttgatttag ccgaaacaat gaatttcacc aaaactgctg atcgacaatt caccacccaa
     8881 ggaaatattt ctaaacagat tctttcgtta gaaacagaat tagacgtcaa attattcgaa
     8941 cgcgcccata gaaaaatcga attgaccgaa gctggaagct tattgctccc atatgtaaaa
     9001 aatgtcgtag aacaatacca tgctatgcaa gaagtgcttg aaacctatac gaaagacaac
     9061 aatctttcat taaatatctt aacgataccg acaatgatca attataaagg tttttccaaa
     9121 atcaccgagt ttttgaaacg ccatcctgaa tttacagtcc agttgaaaga agtggaaagt
     9181 gtcgaattaa cttacaatga cgccaatgaa catagtgaca cgatccactt tgctagaagt
     9241 ttccaggccc caccaagttc gattgagtgg ttgcctactg aaacagacga ttttgtagca
     9301 gttctaccta aaaatcatcc tttagctagt aaaaagaaat tggatctaag cgaactgaaa
     9361 aaggagaact ttttgctcct tggccccact acgaacttgt atcaacctgt actagatctg
     9421 tgtcgcgaag ctggatttga accaaaaatc tcttataaag gagcaagaat cgacttgatc
     9481 atcaatatga ttgcaaatga catggggatt gctattgtta tggaaaaaac agtcaagaat
     9541 ctattaaaag aaaatactgt gattctccct atttctccaa caacagagag ttatcttgca
     9601 tttactcgta aagtcggtga gcattctttg gcttcagata cattttggga gtatttaaat
     9661 caagaagaaa actaagccct acattttctg tccatctatc cacccaaaaa aagagagcga
     9721 cgcatgactt tcgctctctt tcttgcatca tgtccttgtt tcaatgacac ctctaacatt
     9781 tctcgtagtt caataagaaa gcagcgattt ttatgccaaa agaaccgtaa gaacatttcc
     9841 ctaatcacaa aaaaacgact aaaaacccct aaaaaccttc aatagaattt ttaggggttt
     9901 tattcttttt cctattgttt acttaaccgt atcattgctg tcgttcctac tccttcgata
     9961 ctgctataat caatacgtcc atcatgcagt tctagaattt ttttaaccag tgaaagtcct
    10021 agcccattcc cagaaatttg acgacttgta tctccttgat aaaatttttc aaacattcga
    10081 tctctggtct cttcattcat tcctacacct gaatcggtca cttccagtac aatctctgtg
    10141 gctgtctcaa atagtttgat atgaatctgt ccattgattt gattgtactt tatggcgtta
    10201 tccatgatat tcagccacac ttggtacaaa aactcttcat tgcctgtgta atttactgca
    10261 gcaagttcga tatccaattc tagctgttct ttctcccatt taggctgaaa aaaaacgatt
    10321 acttctctga tttgttcatc taatcgatac tctttctttt ccagttggat acgctggttt
    10381 tccagtttgt tcaattttaa gatgttctct gttaattgtg aaagctgcgt gatactttcg
    10441 ataatccgat ataaaaatat ttggcgttct tcgtcagaga gatttggggc ttgtaacaat
    10501 tgaacatatc cttggattgt tgctagtggt gttttgaatt catgggatac actggacaca
    10561 aagtcattcc tcagtgtctc gatactattg agttcttgga ccataacgtt gaaatcttta
    10621 tacagttgct gtacttctgc tactttctgc tgttcatcca ttcgaatcga aaaatctccc
    10681 gttgctacca gactcatatt tgtccctagc gcactgattg gatgcaggat acgccgccca
    10741 acaaaaatcg atattcctgt acccacgacc atactgaacc ccgcaaagac gaataacggg
    10801 tataacgttg gttttccagc cgattcagca ggcgagatag aatgtcgaga caataagaaa
    10861 ataaaaccga gaaaacacag cagtgtcact aagatcgaaa tgaagacaat actagcaaaa
    10921 tagatccata attgtgaaaa tagctttttt ttcatcttta aataaccgcc ttatagccta
    10981 gtccccgtac tgtgatgatt tgaaaatctt ccgtcttttc aaaacgggtc cttaaacgtt
    11041 tgatatgtac atcgatcgtt cgttcttctg tattcgtatc taatccccaa atatcatcca
    11101 tcaactgttg gcgagtgaag atcttgttgg gataagacag taatttatac agaagcagaa
    11161 attctttttg aggcagaatc aacgaatgct gatcttgtaa cactgtataa gcatctagct
    11221 ctaattttgt ttgtccgatt atcaagctac tgctgtaatt gatctttgaa cgtcgaagca
    11281 atgcttccac acgtaggatc atctcattga catcgatggg ttttgtcata taatcatctg
    11341 ctcctaattt gaacccgcgt ttcttatcct caaaatcggc ctttgccgta atcattagaa
    11401 caggtgtgtc aaatctggtg ctacgtaatg agtctagcaa gtcatagcca tccattttcg
    11461 gcatcatgat atctgtaatg attaattcta tacgtgtcgc ttctagtttt ttcagcgcat
    11521 cctctccatt caatgcggtg atgactgaaa agcccactcg ttccaacaca gaacggtaaa
    11581 gcaactgtaa ttgcggatca tcttctacta ctaaaatacg attcattgat ctccctctgt
    11641 tttttgaaaa atttctagat tttttcttgt agttcatctt cagttaacat ttatttcgta
    11701 tactacgatc attgttctga tatacttatt agtataccga atatttcaat acgggagaag
    11761 ggtgattatg cttcaattaa aagatataaa aaaatattat aaagtcggcg aaaccaccac
    11821 caaagcattg gatggtgtgt ctgtcgcttt cagacaaaaa gaattcgtag cgattttagg
    11881 tcctagtggt tctggtaaaa cgactatgct gaatgtgatt ggtggactag ataattatga
    11941 ttccggtgat atggtaatca acggaaagtc tactaaagac tttaaggaca gtgactggga
    12001 tgcctaccga aacaactcta tcggtttcat ttttcaaagt tacaacttga ttggtcatct
    12061 aggcattatt gaaaacgttg aacttgggat gacattaagt ggtgtttcta aagacgaaaa
    12121 acgaaaacgg gcagaagatg cattgcaccg agtcggcttg acaaatcata tgcataaaaa
    12181 gccaaatcag ttatctggtg gacaaatgca gcgagtggcc atcgctcgag cactagccaa
    12241 tgacccagat atccttttgt gcgatgaacc aaccggagcg ttagatacag aaacaagtat
    12301 ccagatcatg gaattgatcc aagagttatc gaaagaaaaa ttagtgatca tggtcacaca
    12361 taatcctgaa ctggcccatc aatacgcaga tcgaatcatt gaattttctg atggtaagat
    12421 cttaacagat tcccatcctc acatcgaacg accaaaagat gatcaattta acttacgcag
    12481 aacaaagatg agtttttgga cagcgttaaa gctttccttc aacaatatcc gaacaaaaaa
    12541 aggacgtact ttcttgacct cctttgcttc cagtattggg atcatcggaa tcgcgatcgt
    12601 tctgtccctt tcttctggat tccaaaaaca aatcgataac acgcaagctg aaacgatggc
    12661 gaaatttccg attactattt ctaaggtaac gactaaccag actcgtgacg atgccggatt
    12721 gggtgcaagc aaagcggatt atccagacag caaaacgatt actgcaaaag tcagcgaaga
    12781 agataaagca cagcacacga ataagattga tcaaacatac gtcgattacg taacagacat
    12841 cgatcctaac ttaagtaata atatcggatt tacccgtact acaggaatca atcttttacg
    12901 tgatgtaaat ggcaaagtac agcctgtcag cttctcgaat caaaatcctg atacggaatc
    12961 tctttcttta tctagtacga tgtcagcaat gacaggtgtg ggagtgtcaa gtttccctac
    13021 acagcttgac actagcaaag aaaacttttt aaaagacaat tattctttac ttgccggaag
    13081 ttatccagcc tcagcaacag atgtcgtgct gatcgtagac ggcaataata atactaatat
    13141 caacgcattg aagaatcttg gatttgatgt gaaagaagac gaaaaacttg attttgatga
    13201 tatcgttggt acaacattca aattagtaaa caacaatact tactatacaa aacttccaac
    13261 tgggaatttt atccctaata cagattatga tgcgatgtat cagaatgctt ctgatgaatt
    13321 gaagatctcg gggatcctac gcgtgaaatc ttcttctaca atgaatctct tatctccagg
    13381 aatcgcttat agcgatcaat taacgactca aatcgtcaac gaaaacaaag aatcagaaat
    13441 cgtcaaagct caaagagaca gtggtgtaaa tgtcttaact actgaaaaag ttgacgaaaa
    13501 tgccaaacaa acattgctta gttatttagg tggagacagc ttgccttcaa gtatcatgat
    13561 ttatccgaac aattttgaag acaaagaaaa gatcttggat tatctagatg attacaataa
    13621 aggcaaatca gatgaagata aaatcattta tactgattta gctggaacga tgactgaact
    13681 gactggcgga ttgatggatg ctatcactta tgtccttatt gcttttgcag ggatctcttt
    13741 agtaacaagt atgatcatga tcagtatcat tacttataca tctgtgattg aacggacaaa
    13801 agaaatcggt gtactaaaag ctttaggtgc acgtaaaaaa gacatcactc gcgtatttga
    13861 cgcagaaacg tgtattttag gtatttcttc tgggatatta ggtgtgttta ttgcatggct
    13921 agctacgttc cctatcaata gcatcttata caacatgacg gatctaaaaa atgttgctca
    13981 actaaatcct gtccatgcga ttattctagt gatcgtctct actatattga cgatgcttgg
    14041 cggtcatctt cctgcacgaa tggcagcgaa aaaagacgcc gctatcgctt tgcgtgcaga
    14101 ataaggttgt gacaaccaca tttagatttg agtcataaga gcgctgaaat agtagaaaac
    14161 agcaagaaac gaggaagtaa tatgttcctt gtttcgcgct gtttttttta attatttttt
    14221 ataattctgg tatacttttc tttcctttgt accacaactc ttctggcgta tacctgtctt
    14281 ttccatttat gaatactgtt ccatctgctt caatcgttac ttcagtccca atccacatat
    14341 tacgagaaag tgcgagaaga ttttttcttt ttcctcttcc tttttttcca aattgcaagg
    14401 catcggttat ctcttctgtc caatgagatt tcacgtattt tacttctccg ttttcaaatg
    14461 taactttcaa aatattcgct atattttcca cttttactgc tttaggaaat gactcctcca
    14521 tttttttcac tttccttcct ccaacactaa gtatgcgctt tcttttagtg ataagtatat
    14581 catagtttat tctattatca aaaaaacgca tccattccat tgtagaatac tttaccgaac
    14641 aagttgaatt gttttcctct ttttcacctt ttactataga taggtaaaga caactaagga
    14701 ggaatctctc atgacagatc aaccgtatga tttaagaaaa gtcatcgaag aactaaaaac
    14761 acttccaggt caatatcacg aaacagatat tgaaatcgat cctgaagcag aattatctgg
    14821 tgtttaccgt tatatcggtg ctggcggtac agtaaaacgt ccgactcaag aaggaccagc
    14881 tatgactttc aacaatatca aaggtttccc cggcacacgt gtgaacatcg gaacaatggc
    14941 tagtcgtaaa cgtgtaggtg aaattttgca ccatgattat cgaacacttg gtcgtttctt
    15001 gaaagacgct gtagaaaatc caatcaaacc tgtgaaagta gacaaagcag acgctccagc
    15061 acaagaagtc gttcatctag ctaccgatgc agacttcgat attcgcaaca ttttgcctgc
    15121 acctacaaat actcctgaag atgctggtcc atacatcaca atgggtgtag tcttaggatc
    15181 agatccagaa aaaacaatga gcgatgtaac gatccatcgt atggttctag aagataaaga
    15241 tacgatcggt atgtacatca tgcctggcgg acgccacatt ggtcacttcc aaaaacaatt
    15301 cgaagccttg aataaaccaa tgcctatcac tatcaatatt ggtttagatc cagcaattgc
    15361 cattggcacg actttcgaac cacctaccac tccacttggc tacaatgaac tatggtgtgc
    15421 aggagctttg cgtaacgaac ctattcaaat cgttgatggt atagcagtcg atgaagtggg
    15481 tattgctcgt gctgaattca tcattgaagc agaaatcatg ccaaatgaaa caatgcaaga
    15541 agatatcaac acgaactcag gaaaagcaat gccagaattt cctggctata ctggggatgc
    15601 taatccagct gtacaagtag tgaaagtcaa agcagttact caccgaaaag acaatccaat
    15661 catgcaaaca acgattggtc ccagtgaaga acacgtatct atggccggta ttcctacaga
    15721 agcaagtatt cttgccttgg tcgataaagc tattccagga aaagtagtaa atgtctacaa
    15781 tcctcctgca ggtggtggta aattgatgac catcatgcaa atccgtaaag aaaacgaagc
    15841 ggatgaaggt attcagcgtc aagcagctat tcttgcttta tcagccttca aagaattgaa
    15901 gacagtcatt ttagtcgatg aagatgttga tattttcgac atgaacgatg tagtttggac
    15961 tttcaacact cgtttccaag gagataaaga tatcatcgtg atcccaggaa tgaggaacca
    16021 tccattggac ccatcagaat taccagaata taatcctggc tctattcgtt tccgtgggat
    16081 aagtgcaaaa actatctttg acggtacagt tcctttcgat atgaaagaac attttgtacg
    16141 tgcaaaattc caagaagtag aagattggga aaaatatttg aaataatgaa tgatctgcaa
    16201 ctgttttgac atcataaaaa ggagtgcgga aaatggaaca attggcacaa cggaagaaaa
    16261 aaatcgtgat tggtgtaagt ggcgcttcag gcaccattta tgccatcaac ctagtgaaga
    16321 aactaaaaga atatccgatg atagaaacac acggaatcat cagtccatgg gcaaaacaga
    16381 acttaaagct ggaatccgac ttatcactaa ctgaattcga aagcctgttt gattaccatt
    16441 attcaaacaa agatttaggt gctgctgtcg ccagcggatc atttttgacc gatggaatga
    16501 tcatcgtacc ggcaagtatg aaaacagtcg caggtatctc tgttggttta ggcgacaacc
    16561 tgattgcacg tgctgctgat gtaacaatga aggagcaacg aaagttagtc attgttcctc
    16621 gtgaaacgcc attaaatgcc attcacttgg aaaacttgac taaattagca cgactaggtg
    16681 tgcaaatcat accaccaatt cctgcttttt ataatcagcc tcaaacgatc caagatcttg
    16741 ttaatcacca tacaatgaaa cttttggatc aatttgaaat tgtaaatgag cattcaaaac
    16801 gttggcaggg tgattaatat ggaaaaagaa acgatagttg aagtaaccaa actaggtttc
    16861 acgatccaag ctgctgttaa agtaatcggt aaagacttat tgatcacttt aacaggcgga
    16921 gatacaccac atatcggaac agtcactaca ttttctcgtg atacagagat gcaaactgta
    16981 cgtttcccta gccacgatgg tcggttccat aaagacgatg tcttagccga aaaaatagct
    17041 gtgatcatca aaactgccgt tccgggaagt tgtacgatca cttccggtat ccatgttgac
    17101 catatctcca atgcacaaat caacgcctct tttccgatgt cagaagagtt aggttacaag
    17161 attttagatt ggttggaaaa aatagatttc catatcaaag aaccagttta ttataaagat
    17221 ggtgaaaaac cattatgaaa gaagaggttg tgaaatcagc gttatgacct gagtatgaga
    17281 gaaaaaaatg gaaaaatagc ttctcatatt ttgccgtttt tgagctaata ccgagggaca
    17341 gctgattgaa cgccgtttat caagaggttg tgataaagcc gcttcgtttc gagtagtaag
    17401 aaaaattttg taaacatggg tttccacatt tttgcataaa tttgacttac taccgagaaa
    17461 ctggcttttg aacaccgttt atcaagaggt tgtgacacgt cttttgtcac aacctctttt
    17521 atttttgctt cactgtaact gctacaatat caccgatttc ttttccgatg tttttacgaa
    17581 tatcttttcg tagtccaagg atgtagcaga ctgaaccatc tggatttttc acgcccatat
    17641 tcacgatact gccttcatac ggcacattgt caaaagttgc ttggacttta actcttccag
    17701 caccgaattc ttttcttata tcatacggaa aaataatata tgccccgccc ttgccgactt
    17761 cggaagcata gatttttgat tcaaaagaat aaatttccct cattttattt ctcctatttt
    17821 ttacatctag tatagcatag caaaaaaaag aaggaactgt gacaaaagtc ttgtcgcagt
    17881 tccttttttc gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatagtc
    17941 aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg
    18001 ctcacaacct cttccttaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    18061 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    18121 ctttttcaca acctcctaca tggtgttcaa aatattattc gccttttgct tctttcacta
    18181 attttgcgaa tgcttgttgt tggaattcag cagctaaggc tttcgtacgt tgttcttgct
    18241 ctgctttgct catttttgga ttttcagtca ttgcttttac cataccgtct tgaacataag
    18301 cttttgcttc actttccatc gtactttgct gtttcttcac taattcagct aacttggctg
    18361 cttggtccgt acttaattct acccatgtct ctgggatttt gaatgtattc actcggctgt
    18421 catattcgtg attgttatca gcaatattga accagaattt atacatgtcg tttttgtatt
    18481 cccaataagt agttttcgtc actttttcag catcgccgtc tgtcttttct actttatttg
    18541 tttcgttatc cgtaccagtt gcttttggtt tttcttgttt ttcatttgtt cggtaaagat
    18601 acactttttc ttttccattc cctaaagctt tatacaaaag catatctgct ccttgggaat
    18661 cagcactgga aacaattttt tctgtttttg tttccgttac tttttccatt ccaaagtgat
    18721 tggacaaatt agcgacgatc aaacttagtg aagcgacgaa gattagtccg aaaatcaaag
    18781 ataaaaaggt ttgccatgtt tttttagcga acacgttcgt taaagcaaaa gcaagcaagc
    18841 tgacaatcaa aactccaata atcatgcatt atcccccgct ttcattagaa caggttcttt
    18901 tttcttcaat aaaaaggcaa taataaagcc aagaatccca aagatgatcg ctacgataaa
    18961 cgcggcatgg taacctgaca gtgtggcatt gattgcctga tctttataag ctaatggcgt
    19021 attatccaat acagatttgc ttgggatatt tgcttttgtc acattcgtca acacactgat
    19081 caaaatagct gtaccaatcg agcttgcgat ttgacggaat gtattattta ccgctgtccc
    19141 atgactgatc aagttatttg gcaatgcatt cataccagaa gtcgttaccg gcatcatgac
    19201 catggagatc ccaaacatac ggatcgcata aaggatcacg atatataaaa ctggagtcgt
    19261 ttttgtcaag aaagcaaatg gtgcagttgc cgctgtcaaa atgaacatcc ctgtgatggc
    19321 taaacgtttc gcgccgtatt tatcgaaaat cgcacccgtc acaggcatca tagcccccat
    19381 gatcaatgca cccggcaaca acatcaagcc agaatggaat gctgattctc cacgaatatt
    19441 ttgaatatac aatggtacga ccatttctgc ccctaccatc gccatatttg ttacaccagc
    19501 taaaacagct gcaatcgtga atgtagatga tttgaataca cgaagttcca agaatggatg
    19561 ttccaaatgt aattgacgcc atgagaacaa ggcgatgaaa atcacaccaa caactaagaa
    19621 accaattact ttcaagcttc cccagccgtc atctcctaca ctagaaaatc cgtaaagtaa
    19681 actaccgaat ccaatcgttg ataaaatagc ggataaaata tcaatgctag gatttgacaa
    19741 tggaataaca cttttcatta ggaagaatgc taaaaccaag acaagaacaa cgattggcag
    19801 taccatacca aacaagtcac gccaatggta gttatcaatg atataaccag ataatgtagg
    19861 gcctaaagca ggagcaagtc caataacgat ccctgacatc cccattgctg atcctctttt
    19921 ttctggcggg aaaatagata gcatgatatt ttgcaataat ggcatcgata ctcccacacc
    19981 gcaagcttgg atcaaacgac cgattagtaa tgtactaaaa ttaggtgctg taaagcaagt
    20041 aatcgttccg atcaaaaaga tcgtcatggc agaaagatat aaatttcttg aactgaattt
    20101 attgatcagc catgctgtta ttgggatcat gatcccgttg actagcaaga aaccagtcgt
    20161 taaccattgc acatcagacg ctgaaatatc gaaagctttc atcaatgtag gaaaagctgt
    20221 cgtcaataag gtctgattca aaacggtaca aaatgtacca attaataaga caacaactaa
    20281 aagtgtccga ttatatggtt ttccgtaaat atctacgggt tggttgttac tcatatttct
    20341 ccttctttct gaattttttc tgaaactact agaatacttt agtcctaaat tcgctatttg
    20401 tcaacctact tgacacataa gttaatacgg atataattaa gggaagcaag aggacaaaag
    20461 gagtgacaaa aatggttggg gcttccctta aacgtttgtt ggaaaatgat caattggttg
    20521 ttggcatcag tgaattaagt gatatcgtag gtgtgtctcc acgtcaatta aggtattggg
    20581 aacaaaaggg attcattcgt tcgatcgaag ctgatgcgaa ttgtcctaga aaatatcgtt
    20641 tacctacagt cataaaagta gaactgatca aaaaatattt agatgacgga tttactttgt
    20701 caaaagctgc agaaaaggca gaaatccgcc aaaagaaaat gcaccatgtc cgtaaagttt
    20761 tttcaaaagc aatcaaagat atcgaattga tcgacgaacg ctatacgatt ttacaaatcg
    20821 gtaacttcaa agaagagaat gctaagatgt acatcattca tgacaacgag acagacgaat
    20881 taacttacaa gctccttcct gtcgatgaaa aagctgacta cgaacagttg ttaaaagaac
    20941 tctaaacgcc actaatctcc taagtaccaa gctttttaat aagtagatgc tacataaata
    21001 cttgaaaaaa ggcctgtttg aacataagaa tatccgaacg gtgatctgcg tttctttcat
    21061 atagaaagga attctccatc actgttcgga tattttttta tttttttcgg ttactttctt
    21121 ctaatacatc tgttagccaa tttgcatatt ctgtttcccg attgatcgca tgcgctaaaa
    21181 tcaggtagtg tccgtaattt tcatgttttt ctttttcagt aggaaagatc gttttttttc
    21241 gctctaatag atgaagtcgt ttttcttcat gaaggtcacg ctgctgctga ataatttcta
    21301 ctaatgccgg atcattcacg tctttgacga aatacaattt cagaacaaat tcgtctcgat
    21361 taaccggtag ttcattggtg ggtgtatgaa tccactctgc caattgttcc ttcccttctt
    21421 ctgttaattc ataaactttc ttttccaatt tttcacctgt gatctctact tgatgctgaa
    21481 tgattccttg ctcttccatt tttgcaagtt caggataaat ctgactatgt tttgcttgcc
    21541 aaaattctcc tatttcattt ttgaacgatt gattcaattc atatcctgtc atcttcttct
    21601 tacttaataa ccctaacaaa atatatcgaa gtgtattttt tcttgccatc agttttccct
    21661 ccttgacaaa tctttaaatc cattctatca taaatgagta atcgaaaaca tgtagtttat
    21721 tacatgtttt aatttataag gagatgctta aacatgaaag aatttaaaga attggaagat
    21781 tttattggaa ctcactttat ctatacgtat gacaatggtt gggaatatga atggtatgcc
    21841 aaaaatgacc atactgttga ttaccgtatc catggcggaa tggtcaaagg ccgttgggta
    21901 aaagaccaag aagctgatat cgttaaattg acggacggcg tattcaaaat cacttggaca
    21961 gagccaacag ggacagacgt ggcattggac tttatgccaa atgaaaagaa acttcacgga
    22021 atgatctttt tccctaaatg ggttgaagaa catccagaaa tcactgttac tttccaaaac
    22081 gaacatatcg cagaaatgga agcagctcgt gaaaaatatg aaacttatcc aaaattactt
    22141 gttcctgaat ttgctacgat cacttatatg ggtgatgcag gtatagacaa tgaagatgtt
    22201 attagcgaag aaccatacga tggaatgcca gatgatatcc gtagcggaaa atacttcgat
    22261 gaaaattata aacgagtaaa ataaggttgt gaaagagagg ttgtgaaatc agcgttctga
    22321 cctgagtatt agagaaagaa acggaaaaat agttcctcat gttttgccgt tttttgagct
    22381 aataccgagg gtcagctgat tgaacaccgt ttatcaagag gttgtgaaat cagcgttatg
    22441 ctttgagtaa taagaacgct agttagaaaa tagcttttca tattttctag gctagtgggc
    22501 ttattaccga gaagcagccg tttgaacacc gtttatcaag aggttgtgaa atcagcgttt
    22561 tgacctgagt attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga
    22621 gctaataccg agggtcagct gattgaacac cgtttatcaa agattgtaac aactgcatat
    22681 tcacacagtt aaattagtga cttcaaaatg aaataatgct cccagcaaaa aattaggctg
    22741 ggagcattat ttcgttattc tgttcatcaa aaatcagcgt tttgattcca tttcatcaca
    22801 agttcctcct catttactgc ttcatctttt tgctgtgttt gaattgtaaa accttgtgaa
    22861 agatagaaat gaatagctga ttgatttttt tgatagactg tcaaagtcag tgttttattg
    22921 gtttctttta tttttttcat tagttgcgta ccgattcttt gttgccggta ttcccttttc
    22981 acaaagattc ctgcgataaa ctgatcaatc accccagcaa aaccaataat ttcttgatct
    23041 atttctgcta agtaaatcac tgcatctggt aaagctttcc taacagattc tacttgattc
    23101 tcccaataag ttgatggaat gaattcatga gaatccaagt tagcatgcag ccaaatcgat
    23161 agaacagatt ccagttcttt ggcagacaat tttgttgttg tgcggatcat tttttctctc
    23221 cttacttatt cattgatttt aaaataaaaa ccattttttt gaagctcttt tacaagtttt
    23281 cgcccctcaa aaaagtatat cttctcttgt tttacttttc taataccaaa agaaacagca
    23341 actttaggat catcagctga aacgattcga taagttccac caggcaattg atccagcttg
    23401 actttcccat gatggtcgct ttgtactgaa aacggcacac catttctagt aagtgttttt
    23461 ttcttatgtt tcaaaatgaa tgaactacca tttaccggtt tccctgacga atcactaata
    23521 ataaatgaaa aatcaagcac ttttttactt aatctatact gatagattag tcgtccgata
    23581 aacactaaca gtatgactac agctattatc gttcctgcaa gaataaacca ttgccgcatg
    23641 gttctttcct gatttccttt tccttcttct ttttttaccg attcgttata aggaatccga
    23701 tgtcctgtca ctagcaaacg atgcgtgttg atcatatacg gcgtacatgt aagcaaggta
    23761 actaagtctt ctccttctga aatcgttaga acagacgtgt catctggcag taccacttgt
    23821 atatttttaa cttgataagc caatttctcc cccaaaacag tcaaaataaa cgtgtctccg
    23881 ttttctaaac gatctaattc ggtaaataac cgtcgattag gtaaaccgct gtgagcagag
    23941 atcactgaat gcgtcgaatt tccaccaagc gggaaagagg ttccttgcaa gacgcctgct
    24001 ccattttcaa ggatcctacc attgagccga tcataaagtg aaatatttaa ttggatctta
    24061 ggtatattga tacttccgat cagccattcc tctaattggc tggatgttgt actttcattt
    24121 ctgttctttt cattgaaagg atcagtacca ggattgaatc cttggtcagt taatcgttgg
    24181 tttttctcct ccattttctt tcgctgcact tcaatttcag ctgcggtccg tttttgtact
    24241 tgttcgatac gtttttgatc aataaaacta tttaaagaat ccacataaaa tggatataaa
    24301 gcgatcagta accctacgac gatacatatt aataaaaaca aacgaaacaa aagtcgacga
    24361 gactgttttt gcttactctg cttcacatgt gtcctccctt gcaagttttt ttgttctttc
    24421 ttttattata tgggttcagg aaaaaaatag aaaatgaaag ccgatgaggt tgtgaaaaag
    24481 ctgtcctgca tcaagtaata agtacagccc aaaaaaatag ctactcatat tttttgttgg
    24541 gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa
    24601 actgcgttat gctttgagta ataagagcgc tagtcagaaa atagcttctc atattttcta
    24661 ggctagtgag cttattaccg agaagcagct gattgaacac cgtttaccga gaggtcatga
    24721 aaaagctact ttgtttcgag tattaagaaa aattctgttt caaaacaagg agatgcaaca
    24781 aaacttatgt cgcatcctct ctccttagaa ctttcactta tgtttttctt gtaaaattca
    24841 taaatatttt catgaatttt ctaatttctt gcctacctct cgcataggat atatgatgaa
    24901 tttatctaaa gtttgcaaag gagttcgaca attgaaaaaa tatggcccat ttttagcaat
    24961 ttttttcatt gctttggcat ttctcatgcc tcatttattc acacataaac taattttggg
    25021 tgctgatgcc ccattccatt acaatcggtt ctatgatgcc gcgcaacaaa ttaaatatgg
    25081 ggatttccat tattttcctt ccctttatgg attccagcaa agtggcagga tcgtgaatgc
    25141 cgtttacggc ccatttttcg cttatttaca aggattgatc gtgttgcttg caggtagctg
    25201 gtttcattat cagattcttt caaatttcat tctttatctt atttcaggat tttctatgct
    25261 gattttactg agatatgtca atgttcgaaa ttgggtaagt gtaccagtag ccgttttatt
    25321 catgagtacg tattctattc aatactggtc tatcaaccaa ggattcacta gctgggggac
    25381 ttgtttttat cctttatgta tgatccctat tgctgatttc gtccttcata aaaagttccc
    25441 tgttatcaaa gtcgctgtct cagtgggact tatgacacag atccatttac tgtcttcgat
    25501 catgttagct ttgatgtata tcccttttta tctttattac ttttttaacc agactgaaaa
    25561 aggaaaagca ctgcttcaac tgattaagtc ggtcttgctt tattttgttc tgacagcaaa
    25621 tatttgggca ggattagcta ttgtttacag tgggaataag attcttcctc cctttgttaa
    25681 cagagaaatg agtcaaaagg cgatagatct ggatggcagt tattggctcc attaccctaa
    25741 ggcctttcct atattgatat tatcaggttt tttgcttttt attttctttc aacaaaagaa
    25801 caccttattc caaaaaatcg tgttgtacac aagtcttgtt ttcttttttc tttctactag
    25861 cttgattcca tggacgacat tgatcgaaaa aaatgtgcct tttattagtt tgatccagtt
    25921 tccatttcga tttttcgtgc catttacgct attgcttcta ctttaccttg ccctttcact
    25981 gaaccagtgg tctgaacaaa agtggttccg tcttttaagt atatttggca ttcttatttt
    26041 ttcagcacag acgatccaga acttatacca gcatctggaa aagtgggaaa atgaaacatt
    26101 tgtcagtcga catacttatc tattcgatac gccggaagaa gcaaggaaaa cattcttcag
    26161 caaagatatg tctaccagct tgttcgtttt ccaaaaaacc acacctgact atctgccaat
    26221 ctataaggag acaaaagaca ataaatacga ccggtataac gaagaaatac tagaaaacga
    26281 aacaactttt ataaaaacac atccaggcgg tcttttgaca atcaagtgga aaaacactgg
    26341 ggacaaaact gttcatatcc ctgttatcgt ctacgatcgg actgtgctac agcaaaatgg
    26401 aaaaacattg actgattatg aagtaacaga tatcgggacc ccaatcgtga agcagcaaaa
    26461 aggaatcaac gagctcacgc ttcattatca aacgccaatc tatttctatt tcattctttc
    26521 tcttactttg attggctggt ttaccttgct atgtttattt atttatcata gatacaagct
    26581 tttacgtgct taggcaaaag ggataaatac atccgatcaa aaaactctcc aaacggtata
    26641 atcgtttgga gagttttatt ttaatcagtt tttttatgtt atatgttata ttttttattc
    26701 gttcacatct aattcgattt ccaccccgta gtgatcactg atgattggac tatcgatccc
    26761 atcaaaaatc cgttgatatt ttttaacctg cattccttca gggacgaaaa taaaatctat
    26821 acgcaatttt tctgtatttt gttcccaacc atctattttc ttttccacag tagctgcttt
    26881 tcctttttca ttcgcgacaa catacgaatc ttgaagaaga aggcaacttt ctcttacagt
    26941 ataataacct tgagagtcta ctggattatt gaaatcacct aggaaaaaaa gtggtacgcg
    27001 agtttccaaa aagtagtttt ccaagttttt ccactcaaaa taaaagccac tttttttatc
    27061 tgtccaccaa gaaaagtggc agctcgagac aaaaatgtcc tgatcatcta tctttgtttt
    27121 cccaatcaag atcttgcgtg tctgataatc ttctttcttt tgaaattggg agacggtaag
    27181 gacctgactt tccaatgttt ttttcgtaag cagtgcattt ccttcttcat atttatcata
    27241 accgatgtgg ctcatttccc aactccaaaa atactctttt ccgtgttcct ttaaaagctg
    27301 aactaaacgg taggcaaaat tatctttacg gataggtact tgattcttta ccggacagaa
    27361 cttcattagc ttcgcaggtt ctacttcttc agattccagt aattggttga tttcttgtaa
    27421 agcaatcact tcataatctt ctaacaggat cttatctgct aattcttgca attttttctc
    27481 tggttctggc tcaagccagc tatgcgtgtt gagtgtcatg aatttcattg gttttctcct
    27541 tgtcttcatt tcgaaacaaa agggttgtga caaaattttt gtcacaacct ctccagcaaa
    27601 tagtgttctc ctgctaactc ctcagtaata agctcaatgg accaggagct attttttgtt
    27661 tggctgtttt tatcacttgg tgcagggcag ctttttcacg acctctccac taaacagtat
    27721 tcaaactgct gcttctcggt aataagccca accaaacgaa aaataggagg agctattttt
    27781 gtttggctgt tcttatgact tgatgcagga cagctttttc acaacctctc cttacatttt
    27841 aatggagccg atcactttac cagctgtcac tgtacctaat tgcttgatca ctacttcttc
    27901 tactttatcg tttgtaaata cgatcatgat cgttggatct ttttcttcgt tttggatcac
    27961 atcaagatcc atctcagcta atttcgttcc tgttcctact tcttggcctt ctttgacagc
    28021 aatctcaaaa gctggctgat tcatttggac tgtatcgatt cccatatgga tcaaaacttc
    28081 gataccatct ttcgtttcca gcccaattgc atgtttgcta gggaaaatac ttgtcacttt
    28141 tccagcgatc ggcgcaacaa cttctcttga tgttggaata acagcaaagc cgtcacccat
    28201 cattttttgt gagaataccg gatcttttac ttcagataaa ggaatcactt ctccagaagc
    28261 tgcggaaact agttcttgtt ctatacctaa gtattgctta ctacctgctt gtccatttcc
    28321 tgtagaaacc atttctggct ctggaatctc tgctcctgat tgcagcagat cttcgatatc
    28381 tgatttcaag acatctgctt ttggtccata aactgcttgg actccattgt ctttgacgat
    28441 caatcccatc gcccctgctc gtttccaggc ttcttcagtc cctacttttt ctcgatcatt
    28501 tacacttacg cgtaaccgag tcatacaagc atcgacatct ttgatatttt cttttcctcc
    28561 cagcaaatga atgatctgga ccacttgctg atctgctgtt cctgttccgc tgttagatac
    28621 aacggtttct tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag caaagttgaa
    28681 ttttttgatc aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga tcacacacaa
    28741 cacgaagttg ataagatctc cacctagacc cgctttgata gctaacggtg tccgagtcag
    28801 caattcgata ttcccaaatg aatggacccg caatggcaag atatctgcca tcgcaaatgc
    28861 agccccttgg atcactgaat aaatgacata taatggcaca gcagcaaaca taaacatgaa
    28921 ctccaatggt tctgttacac ctgttagaaa gacagccaac gctgctgaaa aatacattga
    28981 tttatatctt gctttcttat caggatcgac attacgatac atcgcaaaag ccattcccat
    29041 caagatacct gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg tccagttatg
    29101 caacacaaac tcatattgcg acatatcccc tgcaccttta agattcacta aatcagtggc
    29161 ccaagccaac cataacggat cttgcccaaa tacttgtgtc cccgcctgtg cgcctgataa
    29221 aatctcatac gatccgccaa gctgtgtata gttgatagga atcgtcagca tatgatgcaa
    29281 tccaaatggc aatagtaaac gttctaatgt tccaaataaa aacggtgcta gaatcggtgc
    29341 gctttcttgt gattctgcaa tccataaacc aaaattattg attccagcct gtacgtttgg
    29401 ccacaccaca gcaagaatca atgccacgat tgttgaccat aagataacaa caaacggaac
    29461 aaagcgcttt ccattgaaaa atgacaaagc atccggcaat tttctataat tatagtactt
    29521 attataagcc attgccccga caaatccagc gatgatccct acgaatactc ccatattcaa
    29581 agctggtgct tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg taccgaacag
    29641 agtgtgggta aatgcatctt cattcgcaag catgtcactt gtgacaccaa agatagcccc
    29701 agtgatccga ttgattaaaa taaatgagat cccagctgca aatgctccgc ctgctctttc
    29761 ttttgcccaa cttcctccta ttgctaaagc aaataaaata tgaagattac caataattgc
    29821 ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat caggattgat
    29881 caagggaatt gatttcccta tactgatcat caacccagct gccggcataa ctgctaccac
    29941 aaccattaaa gcctttccaa atttttgcca aaactcaaaa ctaaatatct ttttcattcc
    30001 gtttagctcc tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt tttctttttt
    30061 gtaaatgctt tattttttat ttttctgtct ataccattct ataaaaccta taatacgtgt
    30121 gttcccttct ataattttct cctaaaaatt tattttttta agcttttttc tatgaaatat
    30181 tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta taaaacagct
    30241 cattaggagg agaaaaatga aacagatcaa acgactattt gctattgatc catggaaaat
    30301 cagcacgaca caattagata aaaaaaatct tcgactgcaa gaatctttaa caagtattgg
    30361 aaacggttat atggggatgc gcgggaactt tgaagaaact tactcagggg accaccatca
    30421 aggtacttat ttggcgggtg tctggtatcc cgataagaca cgtgtcggct ggtggaaaaa
    30481 tggttatcca gaatactttg gaaaagtgat caacgccttg aactttatcg cgatagatat
    30541 ttcgatcaac gatcagctga tcgacttggc ttctttggaa ccagaagact tttactggga
    30601 attagatatg aaaaatggtg tattatctcg tacattcaca atcactactt ctactaataa
    30661 agtacgcatt tccttcgaac gttttctgag cattgtcaaa aaagaatcag cttatatcta
    30721 tttaactata gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa aattggatgg
    30781 aaatgttcaa aacgaagaca gcaactatga tgaacatttt tggaatgaga ttgatcgtgg
    30841 aactatagaa gaaatcggct atttgacgac taaaactatt ccaaacaact ttgggattga
    30901 acaatttact gttactgcag caatgcgcca tttcgttaat ggaaaatcag tcatccctga
    30961 ctatcacgaa gatactttag cattacatgg ttgcttgtct gcagaattat ctcaaggtga
    31021 gacatggtca gtggaaaaag aaattatcgt tcttactagt agagatattc cagaagcgca
    31081 gcaagcagaa aaagctgtct cagaattaac gaaccagaca aacaattata tccaagcaaa
    31141 agaagagcaa acagcggctt gggcagaacg atggaagctt gccgatgtgg tcatcgaagg
    31201 agacgaagaa gcacaacaag gtattcgatt caatttgttc caactattct ctacttacta
    31261 cggcgaagat gaacgattga atatcggccc caaaggattc acaggagaaa aatacggcgg
    31321 agccacttac tgggatacag aagcgtatgc tgtccctctt tatcttgctt tagccaaacc
    31381 tgaagtaact aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag caatccacaa
    31441 cgctcagcaa caaggattaa aaggcgcgct ttatcctatg gtcaccttca ctggtgtcga
    31501 atgccataac gagtgggaaa tcacattcga ggagattcac cgaaatggcg cgattgccta
    31561 tgcaatctac aattatacga attacaccgg cgatacggaa tatttgaaaa atgaaggact
    31621 ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt gtccattttt caaaacgagc
    31681 aaataaatac atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca atatcaataa
    31741 caattggtat acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag aaaattattt
    31801 aacttatcaa aaagaagctg ctatcactat cagcgaagaa gaactagcca aatggaaaga
    31861 tatcgcagag aacatgtact tcccagttga cgaagaatta ggaatctttg tccagcatga
    31921 cacattctta gataaagatc tcatgcctgt atccgatatt cccacaagcg atcttcctct
    31981 gaatcagcat tggtcttggg acaaaatcct tagatcttgc ttcatcaagc aggcagatgt
    32041 actacagggg atttatttct tcaatgatac ttttacaaaa gaagaaaagc aaaaaaactt
    32101 tgatttctat gaaccaatga ctgtccatga gtcttccctt tcgccaagta tccattcgat
    32161 tttagcagca gaattaggaa tggaaagcaa agctgtagaa atgtaccagc gaacagctcg
    32221 cttggatttg gataattaca ataacgacac agaagacggc ttgcacatca cttccatgac
    32281 tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc atgatcagtt
    32341 gagattcgca ccatttctgc caaaaacttg gaaagcttat tctttccata tcaattatcg
    32401 cggacgttta ttgttcatct cagtcacaga tgaacaagtt actttcgagt tattagaagg
    32461 ttctcctcta gaaatttacg tctatgaaca gtcttattct ttagcggatc gtctagaaat
    32521 aacgctaaga aaggaggaag caaatgttta aaggggtatt gtttgattta gacggtgtca
    32581 ttaccgatac tgctgaattc cattatcgtg cttggcgaaa gcttggtcag gaaatcggca
    32641 ttacgatcga ccacacattc aacgaacagc taaaaggcgt cagccgagaa gactcactct
    32701 ctttgctttt agcttatggc ggaaaagaac actctttttc aaaggaagaa tttgcagaat
    32761 tagcgaaaag aaaaaacgat tattatttag agatgatcca gacgatcgaa ccaaaagatg
    32821 tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc aagattgctc
    32881 ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa aatgggtctt acaccttact
    32941 ttgatgctat tgccgacccg gcagaagcaa caaacggaaa acctgcgcca gatatcttcc
    33001 tccttgcagc aaaagctgtc ggactaaaag cagaagaatg tatcgggatc gaagacgcgc
    33061 aagccggtat ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg ggacgtgctg
    33121 aagaccttgg agaggagatc ccgcttgtcc ctgatacgac ttttcttacg atcgattact
    33181 tgaaagaaaa gtggcatgac catggataat ccagagatct ccgtcgtacc agttccagag
    33241 actgaaataa agaaaatcac attgaagaac gactacttag aagtcgttct tctcaattac
    33301 ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga aaatgtttta
    33361 ttgtcttacg atcgtttcga agatgttcta acagacaaaa gcttttttgg agcaactgtc
    33421 ggacctgtag ccggaaggat ccgggatgct tcttggggtg agcatagatt ggagaaaaac
    33481 tgcggatcac accatatcca cggtggcacg aaaggctggt ctttccaatt ttgggaagta
    33541 gaagttttca aaactccatc atcaataggt gtggtctttt acttaaaaga tgaattttct
    33601 acctatccag gaccaatcac tgctacgatc acttaccgtt taacaaaaaa cgaattagag
    33661 atgacaacct ctgctagtag ccttgttgaa accatctgca atccgaccaa ccatgcctac
    33721 ttcaaccttt caggaaatgg aacaagagac atctatgaac atcagttaac ggtcttttta
    33781 gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg gaaattggac aaaaaaagaa
    33841 aaactgccaa tcgactttag aaaatcccca acactgcaag agatccttgc atgttatcca
    33901 gatggactag atgatgtatt tcttttgcat catcctaggc tttccaaaac ttccttacaa
    33961 ttattcgaga agcattctgg tcgccaaatg actatagcaa cttctaataa aagtatggta
    34021 ttattttcta cgactggatt cgaagctgat ttctctgtca atggaaaaca gatgcattca
    34081 aactacggat tagcaatcga accgcaagaa atccctgata tcgttcattt ccccaaattt
    34141 ggatcgatca acctccatcc tggtcaagaa cggatcagtc agacgattta tcggttctct
    34201 gcacaataac aagagaaagg tgtttttatc atggctatta ccgtaaaaga tgtggcgaaa
    34261 aaagccggtg ttgccacctc gaccgtctct cgtgtcatca acgatcatcc tagtatttcc
    34321 gagacaacaa agaagaaagt ccgaaaaatt atggatgaac taggttatgt tcctaacatg
    34381 actgcccgca atctaggaaa acgcatttcc agtgccatcg gcgtaatctt accccctctt
    34441 gattcaaaag agcggatcgg taacccattt tatcttgaga tcatggaagc aatcaacgaa
    34501 gaagcgcgga attatgatat gactacagca atcgctactg ctaaaaattt tgaattgctg
    34561 ttagagaacg tccagcgtat gcatttacaa aaacaagtag atggttttat tctagtgtat
    34621 tcagatagtc aagatactgt cattgattat ttataccaaa accacatccc tttcactttg
    34681 atcggacagc cttatcatca tgagaacgaa atcgtttatg tagataatga taaccagctt
    34741 ctaggtaaac aagcaacaga atttttgatc cagaatggac ataagaatat cctgtttgtg
    34801 acaaacacga ctcatgaaag tttgtatttc gaacgatatt tcggttatca aaaagcaatg
    34861 atgatggctg gtcttcctgc gttatccagt gtagtactag agcatccgga ggattatctg
    34921 tcctttgaga aaacgttgaa agaaaacgaa gccacagcat tggtggtcat tgacgatatc
    34981 ttcgcccttc gcacgatgca gttggcgcaa atgtatggct atgtcattcc agagacactc
    35041 tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa cacaccctta ccttacaagt
    35101 atcgatatcg atatctccga attaggacgt gtcgctatgc aaaagctgcg cgaattgatc
    35161 catgaagaat tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat caaaagagag
    35221 acagtcgttc ctttagaaaa cccatactaa atagctgact cactaaatac gctgaccatt
    35281 tatcctatgg tcagcttttt atgtaaaaga agatactact tctaacacat tgtttatctg
    35341 taaaaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
    35401 aacggcggaa gcagaagcaa ctccttcgga aataagctga aattcacaaa aatttaaaaa
    35461 gcaattttct tgagtttctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt
    35521 tttccttcct tcaatttttt acacgtcgac gattcaataa aaaaccgcct aatattgtaa
    35581 agacaggtgc aagaatcgag aaaaaggcga atggcaagta atgcaacgcg ctaactttca
    35641 tcgttcccat aatgaatgtg ccgctgactc cccacggtac gagagaattg acggtcgcac
    35701 ctgaatccgc tagtgttcgt gtcaaatagg tttttgataa gccaacgtca tcaaaagaac
    35761 gcttgaatgt ttctcctgga agaatgatgg acagatactg ttctccaacg atcagattga
    35821 ttccgataca gctcaaggca gtaaacccga ctaaacgaga aggtcggtcc atcttttctt
    35881 ttaattcttg aacgatggta tcaacgatca gatatttgat cagtaaccca cctaatgcca
    35941 atgcaagtaa aatcaaacta gcagaaccta acatactcat gatcccgcct cgagaaagca
    36001 gttcatctag ctttggatca ggtgtatttg cgacataacc attcatcaag atgtctgctg
    36061 cttttccgac cgtcaaactt ttctcatgga taaaagaaag aacgagagca gctgtcgatc
    36121 ccgctagtaa tgtagggata gccggaattt ttttgattgc ccccccaaat aaaataacta
    36181 gtggaatcaa tgtccaagga gagatccaaa agctcgcttt catgttttgg atcatttcat
    36241 tgatactagc tagatcggct gaatgactgt tatgtcctaa tatcgtataa aaaatcaacg
    36301 aaaggacgaa tgctgggaaa tccgtataac gcataccagc gatatgttcg aatagattaa
    36361 ctcctccaat acccgctgcc aagttggtcg tatctgataa aggcgagcag ttattcccta
    36421 aaaaggcacc ggaaacgact gcgcctgtag tcattgcatc attgaatcca agtaaatgac
    36481 cgatccccaa aaaagcaatt ccaatcgtcg aaatcgttgt aaatgaactg ccgacagtca
    36541 ctccgacaat tgcacaaaca agaaatgctg tcggtaaaaa gaaacgcaca gaaacaagtt
    36601 taaatccata aaccataatc gtagggatgg ttccagataa aatccatgaa gcaactaaga
    36661 caccaatcaa aagaaaaatc aggattggaa tgatccctgg agtgattcct ttgatgatcc
    36721 cgtcatggat ctcatcccat gtgaactttt taaatctgcc ataaaacaac aaacacatga
    36781 aagctgcaag gatcgggata tgtggcgaga gctggaatcc gatgatcagt actcctagga
    36841 gtgccaacaa cacgatcaat acagcgatac tttcttttaa agaaatattt ttattcatcg
    36901 tcgatctcct atttcaaatg aaaaggccct gccatgaaaa aactattcac ttggcaggac
    36961 ctcctactaa atcagcgaca aaacgtcggc tgaaagactt atttttttat ttttcttcta
    37021 aacttttttc tacctcaatc ccccaagaat ctttcttgaa gtgattgatg atcaatggaa
    37081 taacgaacat gaccactgtg atagctgcta ctaataatgc atcactcaat gtcgctgtac
    37141 ctaatgtagc aaatacgaca acgatgatag agaagatcag catgcagctg actacccaag
    37201 ctaatccgtt tccgttttta ccaattcggt aaggacgttc gaattctgga tgtttcttac
    37261 gcattttaag caatgaaaca gcaatcaaaa tgtacacgat acaataaata acagttgtag
    37321 catttgtaag tgtcaagaat acagcactta cgtcatccat tacaccataa agcaaagcaa
    37381 ataatgaaat aacgattgat tgtgttagaa cgacatttct agatacgccg tatttgtttt
    37441 ctttatggaa tccgaatttt ggtggaagga atccttcacg agctacttgg atgattgttt
    37501 ttgatggtcc agtaacccaa gctgataatt gtaaaagcac accaataaat accatgaagc
    37561 tgaagatatt tccgatcact tctggtaagc ctaggatttg acagtcaatc aagataggct
    37621 gcgtgatatt tgctaattcc atttttccat ctggtacgac atttgctaca agcatcgcat
    37681 tgatgacatt caataaaact aatccgatca aagcgataaa tacgcctttt gtataatttt
    37741 ttgttgcgtc tttcaaacga ggcatgtaaa cagaactcat ttcgatccca acaaaaataa
    37801 atgtgatacc agctaagtat ttcaatgaat caatatgttc caagtcaggg aaagcttttg
    37861 accatgaaaa gtcacctaga tagccgtttg agactagtcc gactttgaag gctgccaaaa
    37921 cacctaaaac gaacatgatg actactggga tatatacccc tagccataca ccgatgttcc
    37981 cgccgacttt tgccatatcg aatttcaagt tcaagatcgt gatgatccag tagataacta
    38041 aaatacaacc taaaacgaac cagtgattat tgccaagttc tacattacca aatgtgtttc
    38101 ctaacaatgg tcctaaagta gaagcgacca ttaccatacc tgggaacatt tgcacccata
    38161 atagccatgc aacgacaaat ccccatttgc tgcccagtgc tgtttttacc cataactgag
    38221 gaccgccttc ttgaggaagc atcgtggaaa gctcaccgga aaccattgat atcggaccgg
    38281 caaaaaacaa aactgcaaaa attgaataaa aaatcaatgt ccaaccgact gcggctaacg
    38341 taggaatact ccgaaccgtt gcacacagtg ccattgtcag accgataaag gtcccaagac
    38401 ccattttact tacttcagat ttactcaccg ttgacactct ccttgatttt ttggataaaa
    38461 taacggtgtc tttgaagtag tttcagcaat aaacgaaaca attcagcgaa tatttgaggg
    38521 gattctttga gtttattgct tgaagggttt cttcaaagac aacctcaaaa caaagggatc
    38581 gaagagctta ggtctcccct tgctcttcag atccaaacat gcgtcagaaa cagttagtat
    38641 tcaagctgtt ttctaacatg attaccttgc ttattttgct tcgcttgcca ataattggtc
    38701 tgcgtagatt ttttccaatt tttcttgaag agctgctttg attttttcag catattcttc
    38761 aaagtgttct tctttgttca tgtatggtgt cattacgctg gcacgcaata cagttacttt
    38821 tccggcacgg ttccattctt catctgagaa tcctagttgg ttcacaaatt gtaatgggct
    38881 gttgccatag tctgggatag caaagtcagt atgtgaagtt aagaactcat taccgtaaat
    38941 gcttcctttc acgtatgaag aataatcata tacgtcatgg ttcaatttat tcattgcaac
    39001 taagtcatcg ttgccttttt ctttgaatac atagtctacc atattgaaat ctggataagt
    39061 taatggatga acttcgattt ctttgtctcc tactttaaat gacaagtcat tcaagaagtt
    39121 gtagaaacga tgtgcacctt cgattgatgc acccattaat ttaccatatc ctgtgacatt
    39181 caaaggtaag acatggtgtg ctgcccaaac gctggctgct gttgcccctg cttttgaacc
    39241 ttccaagata taagccccaa gtaatgctgg gatatctgcg cctttttcaa atacatatgt
    39301 cgcaaagtaa gagataacgt ctctcatgcg gatatctttg atgacgatac caccggcaga
    39361 atatggaaca tagcccattt tatgtgggtc aatcgttaca gattctgctt cttcgattgc
    39421 tttatatgca ctgtgtactt cttctaagat ataatctttg ttttctgtaa agacattgta
    39481 tttgtaatga acatctttta aatcttcaaa tggaatgaag ttgttgtctt cgtctaagaa
    39541 aatcgcacgg ccgtatccgc cataagcagc atctacgtgt aagtagaagt aaataccgtc
    39601 tttttctaat acgcgacgca atgcaacgat tttatcgata ccgtcgattg ctccttcttc
    39661 tgttgatcct acaacaccga ctacacctag gattggtgtt ttttcagctg ctagaccacg
    39721 aacgattttt tcaagttcgt tgatgtccat gcgatagttg tggtcaactg gcactggaat
    39781 cacttggtct aaaccgatac cgatgatgtc ggcagctttt agccatgaat agtgtttagt
    39841 ttgtggaacc aaccattttc ctaatttttc taaatgtttt ccgctacgtg ctgagtgagc
    39901 tttgatttcg tcgatttttt ctgggacgct gtctaacaga ttcatgattt cttctgttga
    39961 caagttcatt aattcccaat cgctttttcc agcaactagt tcaggtgtca cttctttcat
    40021 tgcaagcggc aatgatttga tgttgcgggc ataccaaagt ccttctaagt ttgctaaaga
    40081 accatcagca acgatgtggc cccagccatc tttatagctc attaattttg caaattccat
    40141 tcctacttct tcttccattt ggcttgttgc aggagaagat tcataggcaa cgttattacc
    40201 attccatagc atagcaaaat tgtacgctag taaagatggc atcaatgttt cagagttcat
    40261 atgaccccag taacgtccgg cattatgcca aggaacggaa tgggtacgca tccgcgctga
    40321 aatttctgat agaacatcct ttgttttatt cacagtgtgt tcaaaactag cactgctttt
    40381 ttcttctggc gtaataatag gcatgtcttg aggcatgtag ttttgacgcc agcctaaatg
    40441 ttcatccacc aattcattca acaaagcttt atagatttgg ccgttctcag ctttgtcccc
    40501 aatgaatagg gcatttaggt ttagatcttt cgacaatgat tcactcatag tgaaaccctc
    40561 ctaaaaaaat atttaaattt tgtcgagccc tacattacac ctgttcttct attctgtaaa
    40621 ctaagttttg gcctgatttt taatcatatt ttaatatgta tcaattatcc gaattgttca
    40681 cacttttgaa ataattaaaa aaaacgaggg aatttacaaa tgtaaactcc ctcgcaaaga
    40741 ttgtattaac gacaaccagc gttctatttt aggatgggtt tcaaaattgg aatgttgtaa
    40801 tagtatttcc ctttttcaag gtaaaaactt actaaattct aatcaattac tttggccaaa
    40861 aagtaatttt tcttaccttt tcgaataacc acaaacttac catcgaattc atcggatgga
    40921 tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa tacttatagc tccgtttgaa
    40981 acatcttcac gtgcttgacg tttagatggt tcaatttttg tggaaacaag cagttccacg
    41041 atattttcag gtgtgcttga aatttcaacg ttcggcattt taccgaatcc ttgcgcgatt
    41101 tcttccgcat ttagttcttt aatatttcca gagaacaatg cttgcgttat tttttgcgct
    41161 tctttcaagt cttcttcact atgcacaaat cttgtaactt cttctgccaa acggcgttgt
    41221 gcttcacgtt tctcaggttc tgtctctact tttttcgcta agtcttcgat ttcttcttga
    41281 gacaagaaag taaagaattt caagtatttc actacatcgc gatcatcttg attcaaccag
    41341 aattggtaga actcaaatgg tgaagttttc ttcggatcaa gccaaacggc accgcctgca
    41401 gtttttccga atttcgttcc gtcagccttc agcatcaatg ggatcgttaa accgaacgct
    41461 tctgcttctg gtccttctaa ttttcggatt aggtctaatc cagctgtgat atttccccat
    41521 tggtctgctc caccgatttg caattgcaca tcgtatgttt tatgcagatg caaaaagtcg
    41581 atagattgca ggatttggta agtaaactct gtaaatgaga ttcctacttc caaacggctg
    41641 gcaacgatat cttttgctaa catcgtgttg atgttgaagt ttttaccgta gtctctcaaa
    41701 aattcaagta aagagatttt tgacaaccag tcgtagttgt tcacaaatgt gatatttgca
    41761 tctttaccaa acaattttct catttgattg gaaagtgcgt ccacgttatg ttgcacttgt
    41821 tccatcgttt gcaacacacg ttctgttttg cggccgcttg ggtcgccgat cgttcctgtt
    41881 ccgccgccaa tcagaatata tggatgatgg cctgccaatt ggaatctttt catcatcata
    41941 aatggaatca aatgtccgat atgcatcgaa tcaccagttg gatcaacgcc gcaataaagt
    42001 gaaatgctct tttcttcaac gagctcgcga agtctttctt cgtttgtttg ctgattaatg
    42061 gcgtcgcgcc aagttaattc atcaataata ttcattttac tgacctccgt gtagtttttt
    42121 tatgaaagca ggaacctgct agcatatgta attaattatt tcttcaggat aggataggaa
    42181 ataaaaaaag tcccttggca tataagccaa gggacgaaat ctcgcggtac cacccaattt
    42241 gtatgtctag atatccaaca tacctctcga acatttaacg ctgtcgcgtg gcaatcactg
    42301 ccaaagactc ctgaaatgta attcgcttta aagcatgtac tggtttgcag cgaccaccag
    42361 ctttctgaaa cattggcttt aacaactact tcgtttcgat cttcgtcttg ttattattaa
    42421 tatgtgtcta tcatagagcg caactttttc tttgtcaaca gaaaaaggat atttttttta
    42481 aattcctcat tttttctttt ttgaatccgc tctaattccg aacattttat tttcaattat
    42541 tgttaaaaga aataactttt tctgtatttt ttctgcaatt tatcgagaag tcactttact
    42601 ttgcctctta ttctttcttc gttacaataa ggaatgggac aagtaaaaaa atgaaaaagc
    42661 aagggacgag gtatatggaa aaaaagcaaa aaattatttt aggagccact tctgctttaa
    42721 ctatcgttat tttaattcta agtttgtttt ttgtcatccg atcatctaca aaaaaagaac
    42781 caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga accgacagat gataagcaac
    42841 aactagttac tcaagcaaaa aatctggctc aaagctacga ttatgaccaa gcgattgcgt
    42901 tattggaaaa agatagtaag aacggatcta ctgaaacaca acagcttctt caaacactaa
    42961 aaaaggagaa agaacaactg atcgactggt ctgatcctac tcagatctct catgtctttt
    43021 tccacagctt gatcgtggac ccgcaaaaag ccttccactc tcaacaggca caaggataca
    43081 aagattatat ggtgactgtt gaagaattca accgttcgat cgaacaactt tatcaaaatg
    43141 attttgtttt agttaatttg aatgatttga tccaaaaaga cgaacaagga aatctttcct
    43201 ttaacgggct tgcgctacct gaaggaaaaa aaccattggt tctttcacaa gatgatgtca
    43261 gctattatga atatatggat aattccggtt ttcctagccg attggtcgta tcaaataaca
    43321 atgaaatcaa aaatgtctat aaagagaaag ataaagaaga aatcggtgac tttgacatgg
    43381 ttcctctgat cgatgccttt gtcaagaaac atcctgattt ttcttatcga ggcgcaaaag
    43441 gtaccttggc cctgacaggt tacaacggag ttttaggtta tcgaacatca aaatctcaat
    43501 atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc taaaaaagtg gcggaacagt
    43561 tgaagaaaga cggctggagc tttgcttccc atacatgggg tcacttgaat atgactcaag
    43621 cttcactaga agatgtaaaa aaagacaatg aattgtggca gcaagaagtt gcacctatcc
    43681 taggaaaaac aaatattctg atttatccat ttggtgcaga catcagcgac tggcagcctt
    43741 attcagcaga aaatcaaaaa tttgcttact tgaagagtca aggtttcgat attttctgta
    43801 atgtcgatgc ttctacacca gcttggggac aattaggtac ggatttctat cgaaatgctc
    43861 ggatcaatat cgatggcatt cgttttcaat ctgatttaga cggaaaaaat cctattcttg
    43921 atccattcat caatgtccat gatgtctatg acaaagaagc acgcgaatct gcttgatttt
    43981 acgtcattta tgaaccaaaa aagccgaact gacattttat cgtcagtttc ggctttttta
    44041 tgctttattt cttcttcggt gcgattccct tgatcaggac ttctgtccat tctgctgtat
    44101 atggaagcag ttttgcgtat atctccggct tagaaattcc ttctaccatt gcttgaaaat
    44161 agaggatcaa gaactcatcc gaatatttca aatccacttt tccttctttc cgccctcgat
    44221 ggaaaagatc aagcatgata ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa
    44281 attctttttt ttcttttttt gtatagtatg tcatcaaatc gatataaaat tgtttgttca
    44341 ctcgattgag ataacggatt ttgttttcac tcatggcaat cagtgtttct tcaaatggaa
    44401 gattttccgc aatcaacgct ttcgcttctt gccctaaatc attcataaat gcaaaaaata
    44461 cttcataaat cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct ttacttacct
    44521 gagcgttctc agctacctta tccattgtca ctccagcggt tcctatgtct gtgttcatca
    44581 agtcaaaagc tgcttgtaaa atctctttct ttttctcttg tgctctcttt tcaaaaccgt
    44641 tcatttgtag agcctcccgt
//
LOCUS       DBKOIJ010000006        42873 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000006 DBKOIJ010000000
VERSION     DBKOIJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.006"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            20..757
                     /locus_tag="ACV5NJ_000237"
     CDS             20..757
                     /locus_tag="ACV5NJ_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484369.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            750..1961
                     /locus_tag="ACV5NJ_000238"
     CDS             750..1961
                     /locus_tag="ACV5NJ_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484370.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            2165..2947
                     /locus_tag="ACV5NJ_000239"
     CDS             2165..2947
                     /locus_tag="ACV5NJ_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HHY6484371.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            3017..3679
                     /gene="trmB"
                     /locus_tag="ACV5NJ_000240"
     CDS             3017..3679
                     /gene="trmB"
                     /locus_tag="ACV5NJ_000240"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HHY6484372.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(3777..5531)
                     /gene="ade"
                     /locus_tag="ACV5NJ_000241"
     CDS             complement(3777..5531)
                     /gene="ade"
                     /locus_tag="ACV5NJ_000241"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HHY6484373.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            5663..6592
                     /gene="comGA"
                     /locus_tag="ACV5NJ_000242"
     CDS             5663..6592
                     /gene="comGA"
                     /locus_tag="ACV5NJ_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HHY6484374.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6549..7598
                     /gene="comGB"
                     /locus_tag="ACV5NJ_000243"
     CDS             6549..7598
                     /gene="comGB"
                     /locus_tag="ACV5NJ_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HHY6484375.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            7595..7873
                     /gene="comGC"
                     /locus_tag="ACV5NJ_000244"
     CDS             7595..7873
                     /gene="comGC"
                     /locus_tag="ACV5NJ_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HHY6484376.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            7848..8318
                     /gene="comGD"
                     /locus_tag="ACV5NJ_000245"
     CDS             7848..8318
                     /gene="comGD"
                     /locus_tag="ACV5NJ_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HHY6484377.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            8290..8637
                     /locus_tag="ACV5NJ_000246"
     CDS             8290..8637
                     /locus_tag="ACV5NJ_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484378.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            8600..9025
                     /gene="comGF"
                     /locus_tag="ACV5NJ_000247"
     CDS             8600..9025
                     /gene="comGF"
                     /locus_tag="ACV5NJ_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HHY6484379.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            8997..9356
                     /gene="comGG"
                     /locus_tag="ACV5NJ_000248"
     CDS             8997..9356
                     /gene="comGG"
                     /locus_tag="ACV5NJ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HHY6484380.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            9528..10535
                     /locus_tag="ACV5NJ_000249"
     CDS             9528..10535
                     /locus_tag="ACV5NJ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHY6484381.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            10560..11744
                     /locus_tag="ACV5NJ_000250"
     CDS             10560..11744
                     /locus_tag="ACV5NJ_000250"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HHY6484382.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12181..13764)
                     /gene="abc-f"
                     /locus_tag="ACV5NJ_000251"
     CDS             complement(12181..13764)
                     /gene="abc-f"
                     /locus_tag="ACV5NJ_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HHY6484383.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(13869..13997)
                     /locus_tag="ACV5NJ_000252"
     CDS             complement(13869..13997)
                     /locus_tag="ACV5NJ_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484384.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(14342..14485)
                     /locus_tag="ACV5NJ_000253"
     CDS             complement(14342..14485)
                     /locus_tag="ACV5NJ_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HHY6484385.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(14708..15187)
                     /locus_tag="ACV5NJ_000254"
     CDS             complement(14708..15187)
                     /locus_tag="ACV5NJ_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HHY6484386.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(15260..15544)
                     /locus_tag="ACV5NJ_000255"
     CDS             complement(15260..15544)
                     /locus_tag="ACV5NJ_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484387.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(15622..15816)
                     /gene="ssrS"
                     /locus_tag="ACV5NJ_000256"
     ncRNA           complement(15622..15816)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACV5NJ_000256"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(15888..17174)
                     /locus_tag="ACV5NJ_000257"
     CDS             complement(15888..17174)
                     /locus_tag="ACV5NJ_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HHY6484388.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            17312..17983
                     /locus_tag="ACV5NJ_000258"
     CDS             17312..17983
                     /locus_tag="ACV5NJ_000258"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HHY6484389.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            17949..18437
                     /locus_tag="ACV5NJ_000259"
     CDS             17949..18437
                     /locus_tag="ACV5NJ_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HHY6484390.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            18450..19397
                     /gene="prmA"
                     /locus_tag="ACV5NJ_000260"
     CDS             18450..19397
                     /gene="prmA"
                     /locus_tag="ACV5NJ_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HHY6484391.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            19398..20162
                     /locus_tag="ACV5NJ_000261"
     CDS             19398..20162
                     /locus_tag="ACV5NJ_000261"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HHY6484392.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            20395..22608
                     /locus_tag="ACV5NJ_000262"
     CDS             20395..22608
                     /locus_tag="ACV5NJ_000262"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HHY6484393.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            22619..23065
                     /gene="dtd"
                     /locus_tag="ACV5NJ_000263"
     CDS             22619..23065
                     /gene="dtd"
                     /locus_tag="ACV5NJ_000263"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HHY6484394.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            23236..24495
                     /locus_tag="ACV5NJ_000264"
     CDS             23236..24495
                     /locus_tag="ACV5NJ_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HHY6484395.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(24584..25531)
                     /locus_tag="ACV5NJ_000265"
     CDS             complement(24584..25531)
                     /locus_tag="ACV5NJ_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHY6484396.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            25925..27217
                     /gene="hisS"
                     /locus_tag="ACV5NJ_000266"
     CDS             25925..27217
                     /gene="hisS"
                     /locus_tag="ACV5NJ_000266"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HHY6484397.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            27232..29001
                     /gene="aspS"
                     /locus_tag="ACV5NJ_000267"
     CDS             27232..29001
                     /gene="aspS"
                     /locus_tag="ACV5NJ_000267"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HHY6484398.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            29110..29985
                     /locus_tag="ACV5NJ_000268"
     CDS             29110..29985
                     /locus_tag="ACV5NJ_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HHY6484399.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            30251..32134
                     /locus_tag="ACV5NJ_000269"
     CDS             30251..32134
                     /locus_tag="ACV5NJ_000269"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HHY6484400.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(32209..32493)
                     /locus_tag="ACV5NJ_000270"
     CDS             complement(32209..32493)
                     /locus_tag="ACV5NJ_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HHY6484401.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(32483..33217)
                     /locus_tag="ACV5NJ_000271"
     CDS             complement(32483..33217)
                     /locus_tag="ACV5NJ_000271"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HHY6484402.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            33346..33969
                     /locus_tag="ACV5NJ_000272"
     CDS             33346..33969
                     /locus_tag="ACV5NJ_000272"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HHY6484403.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            34161..35281
                     /locus_tag="ACV5NJ_000273"
                     /pseudo
     CDS             34161..35281
                     /locus_tag="ACV5NJ_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
     gene            35288..38416
                     /locus_tag="ACV5NJ_000274"
     CDS             35288..38416
                     /locus_tag="ACV5NJ_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHY6484404.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            38435..41629
                     /locus_tag="ACV5NJ_000275"
     CDS             38435..41629
                     /locus_tag="ACV5NJ_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HHY6484405.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(41775..42482)
                     /locus_tag="ACV5NJ_000276"
     CDS             complement(41775..42482)
                     /locus_tag="ACV5NJ_000276"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6484406.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        13861 a         7603 c         9081 g        12328 t
ORIGIN      
        1 aaatggcgga ggaaaaagaa tgagtttaat tatcgatcat gtcacagggg ggtacggaca
       61 tctccctgtg ttaaaagata tcagtttcga tgtacagcct ggagagatag tagggttgat
      121 tggactgaac ggagcaggga aaagtacgac catcaagcac attatcggtc tgctggaagc
      181 ttcaaaagga acgatagaaa tcaatcatct atccttaaag aaaaatacgg aagaatatcg
      241 caaacaaatt gggtatattc cagagactcc ttctttatat gaagaattga cattgagaga
      301 acatatcgaa gtcactgcga tggcctacga tatacctatg gatatagcaa tgaaaagagc
      361 agaaaagttg ttggagactt ttcggttatc taatcgtctt gactggttcc cagctaattt
      421 ttcaaaggga atgaaacaga aagtgatgat cttatgcgcc tttttgatcg agccttcttt
      481 atatatcatc gacgaaccat ttttaggctt ggatccacta gctatcaatg cattgcttga
      541 attgatggta gaaatgaaaa aagaaggagc cggtattttg atgtctacgc atattttagc
      601 tactgcagaa aaatattgtg atcgttttgt ggtcttacat aacggagaga tacgagcaga
      661 cggttctctt gagatgttga gaaaagaatt tcagctgccg gattcttctt tggacgaaat
      721 ctatattgct ttaacaaagg agcaggcaca tgagtagttt ttttcaaaaa cgtttagcaa
      781 gacatcaaaa gaaaatgatg aaatatatgc gttatgtatt gaacgatcat tttgttttag
      841 tttgtctttt cttgattggc ggtgtgggat tatattattc taattggttg aaaaccctgc
      901 agccaccgtt tgcaattgga gggctcataa tctctgtatt ttggatgctc tgtttattcg
      961 tgggtaagat ggctactttt gcagaggcag cagatattgt ttttttactt cctaaagaaa
     1021 aagaaatgca aacctatttg gaacgagctt ttcgctattc ttgcattttt ccgttgatct
     1081 ttttgatatt ggcatgcgga ttggccatgc cattagtggt ggttagtacg gagcaatcct
     1141 tttcgatgtt tttcgtctat ctgatcatat tatggagttt gaaattcagt catctgcaaa
     1201 tcaagagagc agaattgttt cgactagatc aacaagttat tcgttattgg aaattaggct
     1261 ggtttttttc ttctctgatc tttttgtttc tatcatttta ttggctaata tgggcaggaa
     1321 tagcaggtgc attagtacag gcaagtctat attactggtt tttgtggaag caacaagaaa
     1381 aaggattgga ttgggagaaa atggtgcatg tagaagaaca taggttgcat cagatctatc
     1441 aatttatcaa tttattcaca gatgttcctg aaattacggc aaagatcaaa agaagacgct
     1501 atttggatcc tatattgact catattaaaa gagaatcaaa aaacacttat ctttacttgt
     1561 ttgcaagaag attggcaaga ggttctgatt ttagtggtct ttatttccga ttagtaatta
     1621 taggtgggtt attgattgcc ggagtagtcg acttttattt tagtctgatt atcggtttct
     1681 tatttttata tttgatcggt ttccaactgt tacctttagc gaaccagttc cgctatatgg
     1741 ttctcgttca gctttatcca atccataaga atcagaagaa gcaggcgatt cagtatctac
     1801 tcacatgggt attaattatc gcgtcggtta tttttggttt gattgctgca gtcgtattag
     1861 atgggcagca gcgatgggtt ccgctagcgg cttatttgat tgttactatg ctctttgtta
     1921 aactatatgt accaactcgt ttgaagaaga tgtcagactg aaaacaaagc tgtgtagggt
     1981 aaaaatgaga atgaaaaata gagcgttgac agtaagaatg ctgttcgttc tatttttttt
     2041 gagaaaagct ggttttttga atggcaatta aatgaaaaag aacgtatatg tgcttgacgt
     2101 gtagtgttca ggcgactaga ataataagta ctgcaaaaca aagagtaaga ggacgggaga
     2161 atgcatgatg gagtttcagt tagaccaaga gtggcggtta cgtcccatca aaggcgacac
     2221 aggaaaaaca tacataggca tgcgtgatga ggacaaagtc tttattaaac gaaacacgac
     2281 tccgatgttg gctgctcttt caagggaagg aattacacca aaacttgttt ggacaaaacg
     2341 aacggggaat ggcgatactt tgacagcaca agagtggctg gaaggtcgct tgctgactcc
     2401 taatgagatt ggtcaaagaa acgacgtgat tgatgttttg taccatttac atcattcaaa
     2461 ttctcttaaa accatgctga cccgtatcgg tggtcgtgtc atgacgccgg agaagatgct
     2521 gcaaaaatat gaaaaagaat taccggaaga actccagaaa aatcaatttc tgcaaattgt
     2581 ccatcgtttt cttcgtgagc agatcccgtc aatagataag gaaaacttat cagtagtaca
     2641 cggagatgta aaccatcgta attggttagt atgtaaaaat tatttatatc tagtcgattg
     2701 ggattcaatc atgtttgcag accctgcggt ggatattgca acgattttag gaaattatgt
     2761 gccactgtct agctggaatc agtggctgat cagttatggc atacgcccga ctgacgaaat
     2821 gcttgaaaaa tttcattggt atgcggccat gaatttgctc caagaaatca ccagatattg
     2881 caaacgtgaa gatcatcgac ggatgaacga agaaatttta caattaaaac gaattttcag
     2941 tggctgaggt ttggcccaaa ctctgcggag atttggccag acctttttaa ttagaggaaa
     3001 ggaatggaga taaataatgc gtgtccgaaa tcgtccggga gcagcggaaa tgttagcggc
     3061 tcacccgaat tttgtaatta gtgatccaac attgtggaag ggaaaatgga atgaattgtt
     3121 tgaaaacgat catccaatcc acattgagat tggtatgggg aaaggccagt tcatcactgg
     3181 aatggcaaaa gcccatcctg aaatcaatta tattggagta gaaatgcagg tcagtgttgt
     3241 ttcgattgca ctagacaagt tgatcgaaca accgcttcct aacctgaaac ttctacatgt
     3301 ggatggatca gcgttgacag agtatttcgc tgacagcgaa gtagatcaaa tctatctgaa
     3361 tttttccgat ccatggccaa aaaaacgtca tgaaaaacgg cgattgacat ataagacttt
     3421 tcttgctgta gatgaacaga ttcttcgtcc aaatggagaa atccatttca aaacagataa
     3481 tcaaggattg tttgagtatt ctctggcaag tttttctcaa tatggcatga tcctcaaaca
     3541 ggtatggtta gatttacatc aaagtcaatt tgaaggcaat attatgacgg agtatgaaga
     3601 aaaattttct tcaaaaggtc aacgtattta tcgagtagaa gctcaatttc aagataagac
     3661 caaaaagaaa agtctgtgaa actaactttc tgctttcata aaaacattaa tatccataaa
     3721 taaatcccag aaaatgaaca ttacagatgt aatgtatttt ctgggattta tttgatttaa
     3781 aatgtattta tttcatcggt ttttccacaa ggtggaatca attggaagcg agaaaatgaa
     3841 aataatcctt tgtcggtgat cttcaattcg ggtatgacag ataatgttaa gaaagaaagt
     3901 gtcaaaaaag gatcaaaggt tttagctgct cctagttgat gtgctttagt tattaattca
     3961 ttcaactgtt catagacgat cgaaaaaggt tcctgtgtca tgattccgcc tataggaaga
     4021 gaaacacaag ctatttcctg ctgtttgttg acaacggtca tcccgccacc tttttgaatc
     4081 aaaagattag cagcatacag catatcctcg tcgtttgtcc caacgacaac tagattatgc
     4141 gagtcatggg caatcgttgt cgctatcgct ccttgtttca gctgaaatcc tttcactatg
     4201 cctaatccaa tgtttcccgt ttgatggtga cgctcgatca ccgcaatctt taattggtct
     4261 ttttcgattg aaggaatgaa tcgttcgcct tgtcggtcca ctttttctac caaatcttta
     4321 gtcactagac tattcgggat aatttcgatc acatgggctt gtgaagaaga aagaagcagc
     4381 tctaacgaat gtggatttaa tggttgagcg tttagttttg gcagacaaca cgtatatgaa
     4441 tttgaatgat cagcaaataa atggtcttgg agttgcccag attgaacaac taatgttccc
     4501 ttatagaaaa cttcatgaat tggcagcttg gtaagatcgt ctgttaagaa gaaatcagct
     4561 tgatacccag gggcaatcgc acctagtgtt ttcaaatcaa aacattctgc tgcattcaat
     4621 gttgccatct gaatggcttg taatggatcg attccaagtg caatagcttg acggacgata
     4681 tgatcgattg atccttcttt tactaaatca ttgatcagtt tatcatcggt gacaaagaag
     4741 catcttcttg aattttcagg tgtgatcgcc ggtagtagcg atgccagatc ttttgcaacg
     4801 gtcccttctc gaaccattaa atacatacca agatccaaac gttctttagc ttcttttgca
     4861 gttgtagctt catgatccgt ccgaatacct gctgcaggat aaacatttaa ctcttccatg
     4921 ccgataccag ctgcatgccc gtcaattttt ttcttatgct ggtgcatcaa ttggatcttt
     4981 tctaggatgt ctgtttcatt atttgctact gcttgatagt tcatgacttc agctaagcct
     5041 aaaacttcgg gtttttgtaa aaaaggggct aatttttttg catttaaatt tgcaccattg
     5101 gtctcaaaag gggttacagg tacacaagaa ggtagcataa aaaagatatt catgagtgtt
     5161 tgtcttgcat cttctatcat aaactcaaga cctgtactcc cagcaatgtt tcctatttca
     5221 tggggatctg tcactacagt agtaactcca tgaagcaagg atacttttga aaattcgctt
     5281 ggtgataaaa gtgagctttc gatatgcaca tggccatcga tgaatcctgg cactagatat
     5341 tgcccattag cattatgtac gctttttcct tcgtattcac cgattcctac gatcattcct
     5401 tcgcagattg ctacatcttt ttccatgatt tctttcgtaa agacgttgac gatcttagca
     5461 tgcttgatca ctaggtcagc aggctttttt ttacttgcta cttcgatata tttccttaat
     5521 gttgtttcca atggagtgcc tcccaagata aatttttata agtataacat aaattcggtt
     5581 tttgccaata tttttttgta aattcatttt atttgaaaca aaaagatcaa aaagacgtat
     5641 gttttttgtg gaggggagaa taatggaaat aaaacgatta tcggatcaaa tgataagtga
     5701 aagttttgcc aaacatgccc aagacgtata tatattgcca gcttcagaag tattttacat
     5761 ctattttcgc tatggaaaaa aacgaacatt ctggaagaaa gtctcttcgg cagatggaca
     5821 gcaactgatc tcaagattta agtatttggg acagatggat atcggagaaa aaagaaaagc
     5881 tcagttaggt gcatttactt attctttaga aaaacaggaa gttcgtctgc ggttgtcttc
     5941 tgtaggagat taccttcaac gagagagttt agtgattcgg ctattatacg gtacttttga
     6001 aacaccccat cgttgcttta gttctcgaga ttttgcaact attttacaga aaaccgtcag
     6061 acgtggatta taccttttca gcggaccagt cggatcaggc aagacttctc taatgtatcg
     6121 gttggctttg gaagaaaaat tgcaagtgat tactatcgaa gatcctgtag agatagaaga
     6181 accgtttttt ctgcagctac agatcaatcc gaaaatagca cagacttatg atacactttt
     6241 gagactcgca ttacggcatc gtccagactt gctaatcatt ggcgagataa gagatgaatc
     6301 aactgcacat gctgcggtaa gagcagcact aacagggcac cgggtttttg caaccgtaca
     6361 tgcacgcaat cttgaaggta cattggtcag aatccatgaa ctgacaaaga aaaaagaaga
     6421 gctatctgaa tgtttagctg gtgtcattta tcaagaactt ttggaagatg ccaatcacca
     6481 tccttctgtt ttatggagtt atcaatttat ggaggaagaa tcagtaaaaa aatcatggga
     6541 ggaaagttat gaagaagctc aggaaaatat ttggcagtca gaactattct aaaggattaa
     6601 caagaagcca gcaaaaccag tttgcacaaa ccatgggaga actgctttta tctggtttta
     6661 gtctgcaaga atccctttat ttattactca cgatccgttc gtttccacct gttattttgg
     6721 caaatatcca acatcagcta aaggaagggc aagcgtttag tgaggtactt tcgcaaatag
     6781 gatatggagc agagcaactg gtacagatcg agttagctga acgtcatggg aatctgccga
     6841 caactttgtt ggaaattgcc aaacagtttc ggcttgttga aggatttcgc aatgatttga
     6901 aaaagctgct tgcatatccg atgctattgc tcaccttttt gctaggtatc ttgtttagta
     6961 tgcggttgtt cgttttgcct cagcttattg gttcaggaat ggtcatgcaa gagcattggg
     7021 gtattcagct gatacaagtc tttcattggt atgttctact attcatccta gcggttactc
     7081 tattggcttt actagtaagg tatcgtctga tgaagttgtc tcctgttgat cagagtgaat
     7141 ggataagcag acaattattg attggaccga tatattcctt gtatcaatcg gcttatttgt
     7201 cactagagat tggaaagctt tttcatgaag gtttcgaatt aaggcaaatc atagtctgct
     7261 taaaggaaac gaaacaagaa agtctgattc agcatcttgc ctctaagttg attcaaggat
     7321 tggaggcagg tatgtcattg gctgagcagt tccagaatta tcgttttcta tcagaagaat
     7381 tcagtaaaat tgttttacag ggagaggcaa agggaagttt aggtaaagaa ctgatttttt
     7441 acagcgaatg gacaagaaaa cagcttttca aacgtcttca tcactggatg cattttattc
     7501 agccaatcat ttttttgggc gtagcggctt tgattttatt agtttatgcc gctgttttat
     7561 tacctgttta cggaagcata gaggaggttt tgccatgaaa aagagaaaaa tatcttatta
     7621 tagcggtttt acattgatcg aaatgctaat cgtcttactg atcatcagtg tattagtgtt
     7681 gttgttcgtc cctaatttgt cgcgttatag gaatcatgta gaccaagaaa gtcgtgaagc
     7741 gatcatccag ttggtagaca cacaaaaaga gctctatgcg cttcaaaata atggaagagt
     7801 tcctactgtg gaggaattgt tgaatgaagg ctatatcaaa cgagaacatg cagaaatcta
     7861 tcagcggcct tgaaggtaag gttttagcat caggatatac gttgtttgaa tcactcttac
     7921 tgattttatt acttacaacg atcctttctt ttccagttct cgcattttct gcatggaaga
     7981 atgaattggc agtctatcaa tttttcggcc aatttgaaaa acgaatctat tctacgcaaa
     8041 aaatcgctat tgtgaatcag atccaaacag gcttttattg gaatagtgaa gagaatcaaa
     8101 tcatttttca tgttccgaat ccagataaaa taaattggga agtattagat attcctgatg
     8161 agattacctt gaaacgccat gcttctatta cttttgctgc tagaaccggg aatgaaagtt
     8221 cactaaaagc ttatcaattt tattgggaag aaaaagagcg gacgattacg tatcaattcc
     8281 agatagggag tggtcgctat acaaaaagaa ttgaataaaa caggatttgt tttgttggag
     8341 tcccttacag cgcttatgat cgcttctgtc tgtgtctact tagtgagctt tggccagctg
     8401 tttttgcttc aggcagaaaa gaaaagtggg gaggaactgc tatttattcg aatgatttat
     8461 gaagatatca agacgcagcg gatgtatcac aaaagaccag agcgaaggga agaaatgatc
     8521 ccatacgata tccaaattgt cgataataat ggagtaatcg aaaaagtgtt tgtcaatatg
     8581 gagaaagaag cgatcagtat tgaaaaaaca agtaattcca gcatttacgc tgattgaatg
     8641 tctaattgga ctgatgatat tgagcagtct attgctcaca ttcaatcttt tgattcagca
     8701 aaccatccaa gtaagtgaat ttttgcagcg taaagatcaa aaagagtggc taatattttt
     8761 agcacaatta gaagaagaac tcaagagcag tcacaatgtc tctgtgaaaa atcagcaact
     8821 gcactacacg atcgaggata aacagtatat catcgaaaga tatcaggcaa tgatacgtaa
     8881 aagaagaact tccggcggac atcaacctat gttaacaagt atcactgagc ttcaattatt
     8941 ggaaaaagaa cacacaattt ctttttatgt acactttgaa aatggagaag atggatatgc
     9001 catatggaca aaaaacgatc agtagaaaat gttataaggg ctctatttta ttttctgtct
     9061 tgcttttatt tcttttatgt acgtttttat tgatggcagc attagaaagc taccgtttgt
     9121 caatggatct taatatacga atcaaaaatt attatatagc aaaaataatg aagcagatgg
     9181 ctttatcgga acagtctacg cttgccgaaa gttcggaagg agttttttac tacacaactg
     9241 gatctgtaac atatcagtgg gtacaacagt ccctattctt agaggtggaa gtctcaccgt
     9301 ttatctttcg gttcacagaa gaagtaaaaa atgatacaga tactagtaca gaatgatgga
     9361 atgttcgtga aaacgccctc ttttctgtta taacactgtt tttaataaaa aaatgtttgc
     9421 cgtttcacaa ataaaaaaca gtgaaaacga ttgaaatttt gtcaattgtt cgatagaatt
     9481 aaacaggtga aaaaattgtt ataatatatc tagaaatgag gtgcaccatg ttaccagaaa
     9541 aaatggaaca agggtttaat caaaacctgg aagctattca gcttttacag aatgcattgg
     9601 gaacttcctt tttagaagcc tatgtcgaaa atgcagaaaa tttaatagat gattatcaag
     9661 tacgtgtggt ggatggagtg ccgacaaaag agacaacgca acggatcaca gctctttatg
     9721 aagagttgaa gaaactttct ttcgagccag aagaatggcg cagactgtct caattacttt
     9781 tattgaaggg cagccagaca gaacatttgc aggcgaacca tcaactcact cctgatagta
     9841 tcggtttttt atttgttttt cttattgaac aactatacac aaataaaaaa gcaccggtta
     9901 agattctgga tattgcggca ggaatgggca acttgttatt aacagttctt ttaaacctat
     9961 cgaatgcggg gtaccaaaca gaaggaattg gtgtggatat tgatgataca ttgttggctg
    10021 tcgcagcttc tacaagtgat ttgacacaag caaatgttca atactttcat caagacgggt
    10081 tgcaggaatt attaatagat cctgtcgatt ttgcaatcag cgatttgcca atcggttatt
    10141 atccgaatga cgaaaaagca aaagagtttt taacaagtac cgaagaaggc catagttatg
    10201 cacatcattt attgctagag caatcaatga aatatgtgaa gccggatggt tttggtttgt
    10261 ttttgatgcc gagtggattt ttagaaacgg atcaaagtga agaaatcaaa aaatggttta
    10321 aagaagaagg ttatcttcaa ggaatgattc aattgccaga tgaattgttc cgtaataaac
    10381 aatcgcaaaa aagtattttg atcttacaga aaaaaggacc acaagcacaa caagtaaaag
    10441 aagtcttgct tgtgaaactt gcttcattaa aagaaccaga gaaggtgacg gaatttttca
    10501 acgagtttaa aaactggaaa tcatctgctc tgtaatcatt caacgagaag gagagtcata
    10561 tgtctaaaac aattgcaatc aatgctggga gttcaagttt gaaatggcaa ttatatcaaa
    10621 tgccagaaga aaccgtagtt gcgaaaggga tcgtcgaacg tatcggttta caagattcta
    10681 tttttaccat caagtacggc gataatcaaa aattcgaaca aatcatggat atcgaaaacc
    10741 acgaagtagc agtaaaaatg ctgttagatc agttgattga actgcaaatt ttaagctctt
    10801 atgatgaaat caccggagta gggcaccgtg ttgttcacgg aggagaaact tatggggatt
    10861 cagtagtgat cgatgagaaa gtaatggacc gcattgctga attagcagaa tttgctcctt
    10921 tgcataatcc tgcaaactta atggggatca aagcgtttaa aaagatttta cctgacattt
    10981 tgagtgtggc tgtattcgat acatctttcc actcaacaat gccaaagcaa aattatttat
    11041 atagcttacc ggtgtcttat tatgaagatt tcaaagtaag aaaatatggc ttccatggca
    11101 caagtcatcg ttatgtgtcg gaacgtgcgg caaaattatt gaacaaaccg atcgaagaac
    11161 taaaaatcat cacttgccac ttaggaaatg gtgtatcgat cacagcagtc gatggaggta
    11221 aatccatgga tacttctatg ggattcacac cattagctgg ggtgacaatg ggtacacgtt
    11281 caggtgatat cgatccagct gttctcccat atttaatgga aaaattagag attgatattg
    11341 atgaaatgat caatgttttg aacaagaaat ctggtctgct aggcctaaca ggattgtcta
    11401 gtgatatgcg tgatcttgaa aagaactatg aaaaagaaca tattcgttta gcttatgaca
    11461 ttttcgtgga ccgcatccga aaatacatcg gcggttatgt gacaacgatg aatggtgttg
    11521 atgcaattgt ctttactgct ggtatcggcg agaatgacgg acatgtacgt agtgaaatca
    11581 tcaaaggtat gtcttggttt ggttgtgaaa tcgatccagc gttgaatgaa ttgcgtggtg
    11641 aagaagtaga tatctctaca aaagaatcaa aagtaagagt attagtgatc ccaacagatg
    11701 aggaactaat gattgcccgt gatgtggagc gtctgcgcgg ctaagccaaa gcattcgtta
    11761 ggataaaaga tgaaacgcat gcaagaggga attcttgcat gtgttttttt tgcggagaag
    11821 accgcaaaat ggtaatttaa cttcaatttc ccttcttttt tacaggatct tcttagggaa
    11881 ttttgcttgg aaaagtggtc aaaagggaaa agactggcat tttatttatt gattttcagg
    11941 aaaatcccaa tattttattt tcattcatct ttataaggga cattacatat gtaaaaagaa
    12001 aaaacatttt tagatcagcc tcgtgggctg tcgactttat tcttcgcttt atgtttagct
    12061 agaaaaccga tccgaaacat tcattgatcg aataataaaa acttgatttt ccacctgtac
    12121 tttgcctgaa tcgtgaaagc acaggtatga aaatcaagtt ttttgttgcg aaaaaacgtc
    12181 ctagtcaatc agttggccga gcggctggcg gctgatctgc tcctgatcaa taacgagtgc
    12241 ttcttggaag tgttgctgca aaaagtagcg gtcatggctg actgctagga gcgttccttg
    12301 atagtcttgc agcatttctt caatttcttc tcgagcatag ctgtcgaggt gattggttgg
    12361 ttcatccaaa atcaggacgt tgattttttc taaaaagagt ttcagcaacg ccaaacggat
    12421 tttttcacca ccggataaat ctttgatccg tttacccaca tcgctttgat aaaagccact
    12481 tttggctaat tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg cgtaagccag
    12541 caaacggcta tcgggatcgg caaactgcag ctgctgcggc aaataaccga tttgcaagct
    12601 actacctaat ttgatggtgc caccgtaagg cttttcctca ccgagtaata atttgagtaa
    12661 ggttgatttt cctgcaccat tgtcgccgat gatggctaaa tgatctccac gatagactgc
    12721 aaaactgctg tctttgaata gtacttgatc agcgtactgc tggtgcaagt tttgaatgat
    12781 gaaaacttct ttgccagatt ttccaccatt agacaagctt tgcagccgat ttttcggggg
    12841 aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt tccagttctt tggctttttt
    12901 gaagaaggac tcattgtctc cttcatgtgc ccactgacga aaacgacgaa tcatgacttt
    12961 caaccgctgg acttcttttt gctgcagttc gtaatttttt gtcagctcag ccaagcgagc
    13021 ttctttcaag actcgaaaac ggctgtagtt tccgggatac tcaatcaatg caccttcttc
    13081 cagttcccat gtttttgtgg tgacttgatc gagaaattgc cgatcatgag aaacgaccag
    13141 aaccgatttt tttgtattct gtaaatagtt ttccaaccag cgaatactgt taagatctaa
    13201 atgattggtc ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa ttttggctaa
    13261 agcgacttta actcgttcgc ctccgctaag ttctgttaac cgtgcttcct gacggtgtgg
    13321 aatccctaag ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac cgcctagttc
    13381 ttcaaagcga tgctgcagct caccgtaacg ggccagacac ttctctaagt ttgtatctgg
    13441 ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg aggtcctgta tctcagcggc
    13501 agaatggagc aagtagtggt tgacggtcgc ttcggaaact gtaagttctt gttcgaccgt
    13561 cccgatcttt aagccttttt tgcgactgat aacaccgctg tcgacacctt ctctgccggt
    13621 caagatttgt aaaaaggttg atttgccagt tccgttgata ccgactaagg caattttttc
    13681 accagcttca atttgacagg ataaatcctc aaataatgga ttcccgccga ttgttttttt
    13741 aatagaaatt gcttgtatga tcatgatctc tctccttgag accactcaaa aaggccatgg
    13801 ttatccatgg cctgtgttat ttaaactcag attatcatga gaaaaatacc ttgagatggc
    13861 cattgatttt acgtgaattc aactgttttg gacaaacttg tcccgtcaac agcaactgta
    13921 aaaccaattg catggctaaa tgcgatatgg tatcgttggt aattttcatt acttgttatt
    13981 ttagtcatgc gcatcatagt attttccctc cttcgtgtag ttgtcttttc cagtgtacct
    14041 gaaagtgtca gaaagttcaa ctagcgctga catttactta atatttatcg atgtgtaatt
    14101 ctgtcagaga gcttttttta tctgactgag ggtaatgtct cgtggaccac atcattgtat
    14161 tctgagctaa accagcgttc aggcgaaatc atatggtgtt tgataaaaaa ctttgtctac
    14221 atgacttcct tttgccattt gcttaatacc gttgcattaa acagctcttc tctttggaaa
    14281 tctcaccaac atcgcgttca ggagtatcgt tgtggttgca atggcaagcg gtagcctttc
    14341 atcaattctt gaatgattta ccgaatgtag cacttgcctt ctcgcggtac ccgaacccga
    14401 aataaaattt tagtttggcg ataaaatcgg tatccgccat cgatacttca tattttttcg
    14461 tatcagattc atcggaaact tccacattga cgaaccaata agctttggca tgcttaggtc
    14521 gttttaaacc acaccagggc gactaatcgc cctggtgttc actttttttt attttacttg
    14581 aactcattga ctgttttccc tttattttaa acattataaa gaccgatgag tgcttgccgc
    14641 gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt ggcaggtcgt
    14701 ctacgtgtta agctttggtg tggtctttag ctggcaattg gttttctaaa tcagttcgga
    14761 caacaagcac atcgcaagct gcgtttctaa taacatattc agaaacagaa ccaataaata
    14821 aacgttctac agcattcaat cctgtggctc ctaacataat caaatctact tgattatctt
    14881 ctgggatttc tctggcaata atttgttttg gtgagccata ttctacgaca gaggttacat
    14941 tattcaagcc agctttcttc gcattgcttt cataatctgc caatgtttgc tttgccattt
    15001 cagtagcttg ttctgcaagc attccatcaa aggatgaaac ggtttggaaa gaacgtgtgt
    15061 caatgacatg tgctaataga agttctccgt tatttcggat agctacattg accgcctttt
    15121 taaaagctaa ttcagcttca tcagaaccat ctacggcaac catgattttt ttgtattgtt
    15181 gtaacataag cattcactcc tcaagtcaat cgtattgtac tcttttattg tagaacactt
    15241 tagggaaaaa gagaattatt tactgctttg gttcagcaat tttttttgca aatcggatac
    15301 tgaaggtggc agaaacaata attacgatca gtaaaaagac caaggcaata aagcgttgat
    15361 tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc atatgcagct ccgtaaaatt
    15421 gtaggaatcc ttgattttta tcatttttgt tgatcaatac aaaaaagata tcctgtttat
    15481 taaataaata atagctaacg aataataata ctaaagtggc gataatcatt aaaatcctaa
    15541 tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc aagagcaaat
    15601 tgctttattc tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg
    15661 atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc aaatgataag
    15721 gcatgttacc agcagtgaca caagacgggt cccaagatat caataaaaat gttcggtcaa
    15781 cacacaaaga ggacaattcg aacatcacag atttcccacc tctatagtag catatgaatt
    15841 taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga
    15901 ggatgctttt tccactcttt tatacggtaa tactgttgac ccagagcctg ttcatatttc
    15961 cctgtatctt ttggctgata atactgagca ttcttcagtt tatctggtaa atattgctga
    16021 ttgacccaag cctgatcaaa atgatgagga tactgataac ccacgccacg gttgagtgat
    16081 ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa catctccagc tttgccctct
    16141 cgaatatcag caagtgctgc atctaatgcc atgtaggcag aattggattt tggcgacaag
    16201 caaagatcga cgacgacgtc tgctagtggg atacgtgcct caggcaatcc caatttttca
    16261 gcagctaata cagcgttcac tgtacgagcc gcagcagcag gattacctaa acctatatct
    16321 tcataaccga taaccatcag acgccggcaa atacttgcca gatcacctgc ctcaactaaa
    16381 cgggcgagat aatgaagtgc agcatccaca tcacttccac gaatcgattt ttgaaaagcc
    16441 gaaataacgt cataatgagc atctccattt ttgtcatggg taagagcttt tctttggaca
    16501 cattcttcaa tgatcgacag tgtaagatga atccttcctt ctttatctga aggagtcgat
    16561 agtgtagcca gttccagccc attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa
    16621 aggtgaagaa gagcatcttc gtctaactgg attgcttgtt gtccaaggcc gcgttcttta
    16681 tcccttaaag catgttcgac tgccaactgg atgtcttgtt ctgttaatgg tttgacttca
    16741 aagatctgtg tacggctgcg gatcgctgga ttgatggtaa tataaggatt ctcagtagtc
    16801 gctccgatca agatgattcg gccactttct aaatgaggaa gcagaaaatc ctgttttgtt
    16861 ttatctaatc ggtgtacttc atccaataag aggatcactg taccactcat ttttgcttct
    16921 tccgctacaa cttgtagatc cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca
    16981 taattagtag agccggcgat cgcacttgcg atactcgttt ttccagtacc aggaggtcca
    17041 tatagaatca tggaagagag catgcgagca tcgaccatgc ggcgtatgat ttttccttcc
    17101 ccgactagat gctgctgacc aacgacttca tcgatcgttc gaggtctcat gcgataagcc
    17161 aatggctgtt gcataggatt ttcctccttc ttgtttttac gtcccttaat tatatcatat
    17221 ttagcaagat gcctagaaag aacagccttc tgttgttttg ttttcatagc ttatccaact
    17281 aatattgtgt atgataaagt aggtgagaaa aatgacggat aaacaaaaga tagatgaatt
    17341 tttttggaat aaaacgacgc cagaagtggc acgggattta ttggggatgt atttggagca
    17401 tgacacacca gaaggaagat tagctggata tattgtagac gcggaagcat acctaggtcc
    17461 agaagacgag gccgcccata gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta
    17521 tgaaaaacca ggtacgatct acctttacac gatgcatact catcggattt tgaacatcat
    17581 tactcagcca gaagggattc ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat
    17641 gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt ccagatatca gcaacggtcc
    17701 gggaaaatta gtggaagcat tagcaattcc tcaagaactt tatggtcagt ctatagcaga
    17761 tagttcgtta cgcttagttt ttgagaaaaa aaagacacca aaaaagatca atgctttgcc
    17821 tagaatcggt atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc
    17881 gggaaatcct tatatctctt tacaaagaaa aaaccaaatc gagaaagatt ggggttggag
    17941 gaaagaaaat gaaaaagaag gatctattaa catatttaga tgaaataatt gaaaaacaag
    18001 tgacagacta tgatgtagct ttagattggg atacaaaaaa tcatacgatc gagatcgttg
    18061 ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga tgatgcagaa gggatcgttt
    18121 cgcaagaacc aattatcgaa tttgaagacg gcgtacttct ttttaatcct cagaaatctg
    18181 tttttgatga acgcaattat ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa
    18241 aatcagtagc tgattcttta gtggaatatc taaatgaagt attagctcaa gggcaaagcg
    18301 atttgcttga ttttttggat gaagacgatg acgaagctgt attcgagctt cactgggata
    18361 atcagcaact agcagaatta gtagaagtaa aacagcaaaa tgaagcagat acttatttac
    18421 cgtacccaag ttattaagga gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg
    18481 aaagtgaagc agtcgaagca gttgctaata ttttgatgga ggcaggggcg agtggtgtcg
    18541 cgattgaaga ttctttagat gttgaacatt ttcagtcgga tcaatatggt gagattttag
    18601 ataaaaatga ttttacatcg ctgaaagaag gcgcactagt catggcttat tttccggaaa
    18661 cgactttctt accggaaatc ttgcctttta tcaaagatcg tatcacacaa ttacctgagt
    18721 ttggtctagc tattggaaaa aataaagtaa cagttagtga agtagaggaa agcaactggg
    18781 caacagcctg gaagaaatac tatcatccgg tgcgtattac gcgaatgctg acggttgcgc
    18841 caagctggga aaactaccag acagaagatc ctctagaaaa aattatcaca ctagatcctg
    18901 gtatggcatt tggcacaggc actcatccca cgacttcctt gacattgaca gcactagaaa
    18961 cagtcttgcg tggtggtgaa acattgctag atgtgggaac tgggtcgggc gtgctaagta
    19021 ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt tgatttagac gaagtagccg
    19081 ttcgttcggc gaaagaaaat atggatatga atgaaatagc caaagatgtc catgtatcag
    19141 ctaacgatct tttgacagga atcgagattg aaagtgatgt aatcgttgcg aacattttag
    19201 cagatatcat cttattgatg atcccagatg cttggcgttt actgaagcaa acaggcacgc
    19261 tgatcgtttc aggaatcatt gaagagaaga agcaaatggt tctggatgcc atgttagaac
    19321 aagggtttat cgtggatcaa attttccagc aaaaagactg gtatgcaatt attttgaaaa
    19381 aaccagaggt ggaatagatg cagcggtatt ttttaaaaga atcatatgaa tcaaaagact
    19441 attttgtttt agaaggggaa aactatcacc acattgtacg ggttatgagg atggaacccg
    19501 aggatgaagt ttttttggca ttcaatgatc aaatagccat tgttgcagaa atcacggaga
    19561 ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc
    19621 caagttcgat taccatagcg agtggctatc ctaaagggga aaagctggat tggatcgttc
    19681 agaaatccac tgaattagga gcgcaagctt ttcttggttt cccggcatca gcctctgtcg
    19741 ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag attgctcaag
    19801 aggcagcgga acaatcccat cggcagatcg tgccatcagt agagttattg gaaacgaaac
    19861 aagatttatt tgagcggcta gatgattatg acgtggtctt ggttgcttat gaggaatcag
    19921 caaagcaagg cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa ccagatagtc
    19981 gaatcttagc tgtctttgga ccagaaggtg gattatcccg cgatgaagtc gattattttg
    20041 tagaacacaa agggatactt tgtggattag gaccaagaat ccttcgtaca gaaacagcac
    20101 ctctttattt attaagtgct gctagctatc aatgggaatt aatgaatcct tctttcagct
    20161 aacgttcttc attaataaaa gagtatcgac agaaagaatt gaaaaaatag tcaacaattt
    20221 gtataataga aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa
    20281 aaagcgaaca tttccgtaaa aaaacgtata actgcacgga ttttgggaca attatcaaac
    20341 agatatttgt cccgattccc gttttaccga aaaaagaaag ggggatcgat tcctgtgaca
    20401 aaagaagaaa ttttaacggg acccggtgtc ataaagctcg ttagtcaata catgggtccc
    20461 gaacatgtag catttgttga gaaagcatgc gaatacgcga ctgctgcaca tgacggacag
    20521 tttagaaaat caggcgaacc ctacattatt catcctatcc aagtcgcagg tatattagca
    20581 gatttaaaaa tggatcccca tacagtggct acaggcttct tacatgatgt tgttgaagat
    20641 acagaaatca ctttagaaga tctgagagaa gaatttggcg atgacgttgc tatgttagtg
    20701 gacggcgtaa ccaaattagg gaaaataaaa tataaatccc acgaagagca gctagcagaa
    20761 aaccaccgaa agatgctgct agcaatggct caagatttac gagttatcat ggtcaaatta
    20821 gctgatagac tacataacat gcgtacgttg aagcacctgc gagaagataa gcaaagaagg
    20881 atcgctcagg aaactttaga aatctatgcg cctcttgcac atcgtctagg gatcagccgg
    20941 atcaaatggg aattagaaga tacagcactt cgttatctaa atccaaaaca gtattaccgt
    21001 atcgtccact tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt
    21061 gaagatattc gaatagcgac agaagagctg gggatttttg cagaaatcta tggacggcca
    21121 aaacacattt attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt
    21181 tatgacttgc tagctattcg agtgatcgta gattcaatca aggattgtta tgctgtatta
    21241 ggagcaatcc acacaaaatg gaaaccaatg cccggcagat ttaaggatta tattgccatg
    21301 ccaaaagcta atatgtacca atctttgcat actaccgtga tcggtccggc aggaaatccg
    21361 gtagaaattc aaatccgaac acaggaaatg catgaaatcg ctgaattcgg ggttgctgca
    21421 cactgggcct ataaagaagg aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa
    21481 ttaagctggt tccatgagat actcgaactt caagacgaaa gctatgatgc ttctgaattt
    21541 atggaaggcg taaaaggaga tatctttagt gataaagtct acgtcttcac accaaaagga
    21601 gacgttactg agttaccaaa aggatccgga ccattggact ttgcatacag tatccataca
    21661 gatatcggta acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca gcttgattac
    21721 aaattgaaaa acggagatat cattgagatc atgacttctc caaattcatt tggcccaagt
    21781 cgcgactggt tgaaattagt tgctactagc aaagcaagaa ataagatcaa acgtttcttc
    21841 aaagcccaag atcgagaaga aaatgtgatc aaaggccacg aatccgtggt caaatgtatt
    21901 acagatctag gatttacgcc taaagatatt ttgacgaaga acaaactgca agaagcactc
    21961 gatcgtttta attatcaaac agaagatgat ctctatgcag ctgtagggta tggagaagtt
    22021 agccccttga cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag
    22081 cagcaaaagc aagaagcaga agaaatcatg aatcagccga aaaaagaacc tgacaaaatg
    22141 aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag aaaacttact aattcgtatc
    22201 agtcgctgct gtaatccgat tcctggtgat gatatcgttg gttatatcac taaaggcaga
    22261 gggatatcca ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc aaatgtagca
    22321 gaacgtttga ttgaagtcga atgggaagat acatcgaata cacgaaaaga gtatgatgca
    22381 gatttggaaa tttacggcta taatcgttca ggcttattga atgatgtact tcaaacagtc
    22441 aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga cgaataaaga taaaatggcg
    22501 acgatccatt tgacggttgg tatccagaat ttatcccacc taaagagtat cgtggataaa
    22561 atcaaagcag tacctgatgt ctacagtgta cgccggacga atggatagga ggacagatat
    22621 gagagcagtt gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg
    22681 aaaaattggc tacggcctac ttgttttatt aggcattcat gaaaccgata cgcaaaaaga
    22741 tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa
    22801 aatgaatatt agtgtagaag atgtgggagg acaaatttta agtatctctc aattcacact
    22861 gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac gaccagaaac
    22921 agctattcct ttatacgaat cattcaatga aggaatcaga agtcgcggca tcacagtcgc
    22981 taccggacaa ttcggagcag atatgtcggt ttctctcgtc aatgatggac ctgtaacgat
    23041 cattattgat tctaaaaaca aataaagtaa tctggttttc actagtaaaa actatgaaac
    23101 acgtcgctta agttagactg ataaactcta acttaagtgg cttttttgtc tgcaaaaagc
    23161 gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt tattttttat
    23221 aggaggttgc tgaagatgaa agaagtacca gtagtagata tcaaaatcac agatttaaaa
    23281 aaattgtatc aaaaaggata caacatgttg gaggagttac gccatgaagc ggacgctccc
    23341 gtcgtcaaag caaagatttt caataaagaa gctatcacga tctacggatc atcagcagca
    23401 aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg
    23461 aagacacttt ttggtcaagg tggagtacag actttggatg gagctgctca tcaccatcgg
    23521 aaaaatattt ttatggatct aatgacaccg gaaagaatgg aagattatca tcgtatctta
    23581 gataagaatc tgacacaagc acttgaagca cagcatggtc aatttgaact atttgattta
    23641 agtaagatgg ttttctttac atctatttgc gagtgggcag gtatcaattt atcagcgatt
    23701 tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag cggaacgttt
    23761 acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga aaaatgggcg
    23821 caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta
    23881 tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga agtggcagct
    23941 gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg
    24001 ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga cttttcgacg
    24061 ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc aatgctacct
    24121 gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg cagttgggtt
    24181 attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc agattccttt
    24241 atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga atatgagatg
    24301 attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga atggatcact
    24361 cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt ctctgtacct
    24421 gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag ccgagcgcta
    24481 ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa acagtgctga
    24541 tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat ccacgaaatg
    24601 cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct tggacattgt
    24661 cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt ggattcttca
    24721 tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca atggggcgaa
    24781 gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct ggatttcttt
    24841 ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt cctgtcagca
    24901 aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca agataaggga
    24961 ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact acatcgacat
    25021 atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc
    25081 ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt tttggtgtgc
    25141 tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg
    25201 catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc cctttatttg
    25261 gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct ttttccttca
    25321 gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg gtatcgatca
    25381 aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca tcttccaaac
    25441 cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga
    25501 cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg tctagtctaa
    25561 aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt cagatatctc
    25621 ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg
    25681 agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat ctaaagtttc
    25741 attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta
    25801 ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga atttcattca
    25861 cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa agggagagaa
    25921 aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa
    25981 atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt ataatgaaat
    26041 gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg atacaacgga
    26101 tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg tgacgcttcg
    26161 accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt tcggcccaga
    26221 gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg aacgccctca
    26281 aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat cagaaaaccc
    26341 tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt tggggatcac
    26401 gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag cctatcgtca
    26461 agcattgatc gactatcttt taccttttga atcagagctg agtgaagact ctaaacgccg
    26521 ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc aaaaatttgt
    26581 ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac attttgatac
    26641 cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta atatggttcg
    26701 cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac caaaaatggg
    26761 ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag aactaggtgg
    26821 tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct tgactatgga
    26881 ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg
    26941 agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact ttggtttttc
    27001 agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag cagataaagc
    27061 gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa cgattaatgt
    27121 caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat atgaacattt
    27181 tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata aatggcacaa
    27241 agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt tacattaaaa
    27301 ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct acgtgatcgt
    27361 gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg
    27421 gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag acgcggagaa
    27481 aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag tgaaatcgtg
    27541 attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc aatcagtgac
    27601 gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc
    27661 aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa tgactttctc
    27721 gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga ctacctagtt
    27781 ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca attgtttaaa
    27841 caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg tttccgcgat
    27901 gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga aacaacgttc
    27961 ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt aatgaaagaa
    28021 gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga agcaatggct
    28081 cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga tctaagcgat
    28141 acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg
    28201 aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat ggatcagtta
    28261 ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt agaagaagac
    28321 ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat cattaaagca
    28381 acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga aatcgtcgct
    28441 gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat tgatgaatcg
    28501 aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga agaagaaggt
    28561 cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat cccgttattg
    28621 gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa
    28681 ttaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt actagaaact
    28741 ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac agctttagat
    28801 tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc aatgttatta
    28861 gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa agcagcagat
    28921 gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt gaatattgat
    28981 gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga atacgcttct
    29041 tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca tataaagcgg
    29101 ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac gaccaagctt
    29161 tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt ttatcagcca
    29221 ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac tttgtcaaaa
    29281 tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa tatacctttg
    29341 tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt tatcacagtc
    29401 actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac cgatccaatc
    29461 atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc gttgagaaac
    29521 ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag
    29581 tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc cggaattcta
    29641 tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact
    29701 gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa acatccagaa
    29761 aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat caatgaagca
    29821 gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac acgatacgaa
    29881 ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt aagtattgca
    29941 gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga gtttgacaaa
    30001 aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt tctaccatag
    30061 tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt gaaaaaaagc
    30121 atgtttattt ttttaaattt atgggatgat agaaaaaaat agtagagaaa ggacacgatt
    30181 ctacttgcta ttttcactta atacccgtat attacgaatg aaaaaagttt atgaaaagtt
    30241 ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca ctgggtttga
    30301 tgggctccgt tcattagctg tggtgggtgt tatcttctat caccttctgc caactagtat
    30361 gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta tctggttatt tgatcacgga
    30421 tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat atttggcaat tttatgtcag
    30481 aagaatgaag agactctatc cggcacttgt ttttatgctt gtgacagctt cggcttatat
    30541 cactttgttt cagcgagggc tgttaaacaa tcttcgagga gttgtggtca gcagcttact
    30601 gtatgtcaac aactggtggc aaatcaataa cggtttatct tatttcgatc gatttgctaa
    30661 tgagtcaccg tttacgcata tctggtcttt agcagtagaa ggtcaaaact atttggtttg
    30721 gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga tcttttatac
    30781 tgtgttaggt acatccttcc tctccgccat cttgatgatg atcttgtata caccaggagg
    30841 cgatccaacc cgtgtttatt acggtaccga cactcgtctg ttttctatat ggttgggaag
    30901 tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa aacatcccga aacaagcgaa
    30961 aaatattctg aatatcgctg gaggaatctc gcttttggca ctgattcttt gcttcttctt
    31021 tttagatgat cattacaatt tcatctatta cggaggaatg ctcctagtga gtctgttatg
    31081 cgtagtgcta gttgcagtga cagcacaccc cggagcaagt ttgaatcgat ggctgacaaa
    31141 tccagtattt acttggattg gcaaacgaag ctatggcatt tatctgtatc agtttccagt
    31201 tatgattttt tatgaagcaa aggtgactga tattgccgat catgttttgt tgcatacttt
    31261 agtggaaatc acattgattc taatcatcag tgaactttct tatcgatatg tggagcgtcc
    31321 gctagcacgt tttgattatg gtcgtacatt ccaagtgatg aaaggatggt tcagcaaacc
    31381 gatcatcagt aagagcaaac catgggttgt tccaggcaca ttacttgtag caattgcact
    31441 cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac aactgcaggc
    31501 aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga aacaattcag aaaacaccac
    31561 tagttcggtt gaccaagcga tccttgataa gtacgagttg tccaaggcac aagggaataa
    31621 ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt atgttggatg ctgcaacgga
    31681 tctacaagaa ctttatccaa aagtagtggt agatggagat gtcggacgac aattatatgc
    31741 cagtgtaccg tacattgaaa aattaaagga acaagatttg ttgaaagaca ctgttctgat
    31801 tggactagga acgaatggct cattctcgga agctcagttt gatagtgtga tggatgcaat
    31861 cggtgatcga aaagtatact gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga
    31921 cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac aatatgacgc tgattgattg
    31981 gtacagctac agtaatgacc atagtgactg gttttatgat gatcaagtcc atccaaatcc
    32041 tgaagggatg acacattaca ttcacttagt cagcgaagcc ttgctaggag gaagttcaag
    32101 tacgaacagc agtgagacaa acgtgacaga atgatagcta caaaaaactt caggcaaaaa
    32161 catttgtttt tgcctgaagt ttttttatat ttaccgtgtt tttaacattc atatagattc
    32221 aggaagcaaa acggttgagt gtgtctgtaa ataacgttct ttttgttttc gggtgagttt
    32281 tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat
    32341 catcttaaca ggtcttcttt tttgaagacg agtatattta gcaccagtac cattgttatg
    32401 ttcctctaat cgtctagaaa gatcggttgt atatccacca taaaatgtct gatctttaca
    32461 gaataatacg taaaaataat gttcatttgc catagagcat ctcacgaatc tctgcagtgt
    32521 attctccatc catattgtaa gtgatcaatg gtggagcgac tttgaatccg tctaatttgc
    32581 cttctttgat tccttcaact aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa
    32641 attggatcct tttaggtgca attcgatagt cttccatgac ctgaatgatt tctaagaacc
    32701 gatcaggacg atggaccata gtaaagcgtc catttgtttt tagtagctta cttgaaatat
    32761 cgatcacttc ttctaacgtc gtatgaatct catgacgggc gattgccagg tgtttattag
    32821 ggttcgtttt atttgttggc agccctttga aataaggtgg attacagacg actaagtcac
    32881 aagaattatg tcgaacgaca gataagctgt ctttcagatc gatggtgtgg acagtcattt
    32941 gttcatttaa atggttaagc gcgatactac gtcttgccat gtctgctaga cgaggttgaa
    33001 gttcgatagc gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca actgcgccat
    33061 ttcctgcaca aaggtcaaca atcttccctt ttttaggtac acgggaaaag tgagcaagca
    33121 gcacagcatc taaagaaaaa gaaaacactt tggaactttg gataatcttg atatcttctg
    33181 cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa
    33241 atagtcgtct gtattatact atttttatta aaaagaacca aggtggcttg tcaaacctct
    33301 attttttatg catactatga ccataagaag ggaaaggaag agactatgtt ttatcgattt
    33361 atgcgcgggg tagttcgagt actattagcc ttagttaatg gaaatgcccg ctatgagaat
    33421 aaagaagttt taccgcaaga tgagaattac atactagttg ctccccatcg tacttggtgg
    33481 gacccattat atctggcggt agctgcagca cccaaggaat tcagctttat ggcaaaagaa
    33541 gaactgttca aaaatccagt tctgcgattt atattaactc gatccaatgc ttttccagtt
    33601 aaaagagaaa atccaggacc aagcgtcatt aaaacacctg taaagatttt gaaatcgaca
    33661 aatttgagtt tgatcatgtt tcccagtggg acaagacatg ctacagaact taaaggcgga
    33721 atggcattga tttccagaat gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg
    33781 ttgacacttg gggatctgtt caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc
    33841 gacttatcag atatcaagaa aacggataaa gaaggtatgg cggagatcga acgacgaaca
    33901 caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa
    33961 aaaaaataaa atgataagtt tatgcgtggg cggtcttttg aatctactgt cctgttcagt
    34021 tcagactttt tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt ttgcgttcca
    34081 atatgtaaaa tagaagacag gtgtcatgta atggatcaaa gtgttataat aattagaagg
    34141 acattttttg aggtgattga atgcgttttt tacatacagc cgattggcat atcggaaaaa
    34201 aactacaagg ttatgacttg attgaagacc agcgtcatgt actatcttct atactagata
    34261 tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg agacttatat gataggagtg
    34321 tcccagctgt tgatgccgta gaattataca atgaattgat ggtcgattgg aatttaaagg
    34381 aaaagatccc gatttttgct atatcaggaa atcacgatag cagtacgaga ctttcagcgg
    34441 gtacgccatg gttcagttat tcgaactatt ttttgcgaac acagctagca gaagcatttg
    34501 aaccagtcga gtttaaaaac gtacaatttt tctttcttcc ttactttgaa ccaattgcag
    34561 cgagattata ttttgaagat gacaatatcc gaacgatcaa gcaagcggta gccaaggtga
    34621 tagataaaat gcaggagcta tttgattcag aaaaaaacaa gtattgatta gccatttttt
    34681 tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg ggttggattc
    34741 gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt tgcataatca
    34801 agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat tctctttatc
    34861 tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc tggagttgaa
    34921 ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt tcaaagaatt
    34981 gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata tctatctcac
    35041 tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc ctcgtatttt
    35101 ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga
    35161 aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg aagagcagct
    35221 aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg
    35281 atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta tcaatgagac
    35341 cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga agacaggagc
    35401 tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa cttccggaag
    35461 acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt
    35521 gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac cagaacaagt
    35581 tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa cgttgaccat
    35641 ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag cagatcagtt
    35701 gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt tactacccca
    35761 aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg ttttacgtca
    35821 tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca
    35881 acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa aaaggtttgt
    35941 tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc aatggacaaa
    36001 ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa cggaacttca
    36061 ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga aaaaaagtta
    36121 tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa aagacgaaat
    36181 agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag tcgaaccaat
    36241 ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca
    36301 aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa
    36361 ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa acaaacagat
    36421 gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac aattaacaat
    36481 ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg ctatctatca
    36541 ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc aagaagatcg
    36601 tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag aaaaacaaaa
    36661 agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag caactgaaga
    36721 aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac agctgaaaag
    36781 ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag gggagccatg
    36841 tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac agccaaataa
    36901 ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg tagaaagaac
    36961 tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac aattatcaga
    37021 tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag aacacttgca
    37081 acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa caactcttgt
    37141 agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa gaatagagct
    37201 ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct acaaacagca
    37261 gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa gtttagaaca
    37321 acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac ttgaaaaaca
    37381 gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc aattgttgca
    37441 agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag aaaaaataga
    37501 tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc agtcgtattt
    37561 ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata aagaaaacca
    37621 acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc tgcgtcaatt
    37681 agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc aacagttaga
    37741 agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac aagaacaaaa
    37801 aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa gccagacgaa
    37861 attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa
    37921 gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt tacaagtagc
    37981 aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag cggataccat
    38041 tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc aggcaggtat
    38101 gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac tttctttagc
    38161 attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg atgcattgtt
    38221 tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa tggaagcttt
    38281 agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa gagaattgaa
    38341 agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa gcaaaatacg
    38401 ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc cagaaaaagt
    38461 cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg tccctgacca
    38521 agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga
    38581 tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac ataaatattg
    38641 taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc gtatcatgaa
    38701 aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa
    38761 aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt tgattattga
    38821 ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta ttttggcact
    38881 ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta
    38941 tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac agtaccagtt
    39001 tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg
    39061 aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag gaaaactaga
    39121 taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc agatgaatca
    39181 gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac aattttccga
    39241 tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt tgctgcaagc
    39301 attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc aagggacatt
    39361 ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat tgattcgccg
    39421 attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat
    39481 tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag aagaggaagt
    39541 agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc
    39601 tcgagtcgat ttcgtctatg gtgaagtcat ctattcgact gacccgaagc atgaggtctc
    39661 gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag tacttgatct
    39721 attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat tgccaagcgg
    39781 ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt ttggtgaagt
    39841 cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc aaccgaaaat
    39901 cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag ggatagagga
    39961 gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt atacattaga
    40021 aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc aagtgttgca
    40081 acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta aaaatcaagg
    40141 attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat cgttccaaac
    40201 gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca aaggattgaa
    40261 tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta
    40321 tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac agacaattgc
    40381 ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg tagcgcctgc
    40441 gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt taagtatcca
    40501 agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca cagatatccg
    40561 agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc aaaaaattga
    40621 ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc
    40681 attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa tcgagaataa
    40741 tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc cgactaaaac
    40801 aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt ttattttacg
    40861 gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga atctatattc
    40921 tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga
    40981 aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac ttgctggtct
    41041 gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt accaaggcgg
    41101 ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga acaaacgaag
    41161 agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag agttagccga
    41221 gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc ggttaagtat
    41281 ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa aggctggggg
    41341 aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt ggtggaatcc
    41401 tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga
    41461 agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac tccagcaaga
    41521 aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga cgaaactgac
    41581 tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag agactcgaaa
    41641 aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat ttgatttaaa
    41701 aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca tttttttatt
    41761 tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg atcaaaagaa
    41821 gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg gttgaaccaa
    41881 agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt atcgccaacg
    41941 tataaggtct cttctggaag catatgaaat tctttctcag ccagctgaaa aatctctttt
    42001 tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg aatccaattg
    42061 ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt tgtgataatt
    42121 cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag
    42181 cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc ttcttccgta
    42241 atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg catatattct
    42301 agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg gcggaaccgg
    42361 atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt gaccgcgttt
    42421 cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac tgatttaatc
    42481 aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga ctattgtaac
    42541 agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat ttctatttcc
    42601 ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga aacacgtcaa
    42661 taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt ttaatgcaat
    42721 ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa ctattcaaac
    42781 ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta atcattgctt
    42841 taaaatacta cgcttatata acagagaaaa taa
//
LOCUS       DBKOIJ010000007        42079 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000007 DBKOIJ010000000
VERSION     DBKOIJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.007"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            152..799
                     /locus_tag="ACV5NJ_000277"
     CDS             152..799
                     /locus_tag="ACV5NJ_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HHY6484407.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            814..2340
                     /gene="zwf"
                     /locus_tag="ACV5NJ_000278"
     CDS             814..2340
                     /gene="zwf"
                     /locus_tag="ACV5NJ_000278"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HHY6484408.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2389..5187)
                     /gene="ileS"
                     /locus_tag="ACV5NJ_000279"
     CDS             complement(2389..5187)
                     /gene="ileS"
                     /locus_tag="ACV5NJ_000279"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HHY6484409.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5449..6156)
                     /locus_tag="ACV5NJ_000280"
     CDS             complement(5449..6156)
                     /locus_tag="ACV5NJ_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HHY6484410.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6177..6959)
                     /locus_tag="ACV5NJ_000281"
     CDS             complement(6177..6959)
                     /locus_tag="ACV5NJ_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HHY6484411.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7008..7286)
                     /locus_tag="ACV5NJ_000282"
     CDS             complement(7008..7286)
                     /locus_tag="ACV5NJ_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HHY6484412.1"
                     /translation="MALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLAKIC
                     EPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7310..7909)
                     /locus_tag="ACV5NJ_000283"
     CDS             complement(7310..7909)
                     /locus_tag="ACV5NJ_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HHY6484413.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7923..8600)
                     /locus_tag="ACV5NJ_000284"
     CDS             complement(7923..8600)
                     /locus_tag="ACV5NJ_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HHY6484414.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8617..9858)
                     /gene="ftsZ"
                     /locus_tag="ACV5NJ_000285"
     CDS             complement(8617..9858)
                     /gene="ftsZ"
                     /locus_tag="ACV5NJ_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HHY6484415.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9881..11206)
                     /gene="ftsA"
                     /locus_tag="ACV5NJ_000286"
     CDS             complement(9881..11206)
                     /gene="ftsA"
                     /locus_tag="ACV5NJ_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HHY6484416.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11362..12582)
                     /locus_tag="ACV5NJ_000287"
     CDS             complement(11362..12582)
                     /locus_tag="ACV5NJ_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HHY6484417.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12597..13685)
                     /gene="murG"
                     /locus_tag="ACV5NJ_000288"
     CDS             complement(12597..13685)
                     /gene="murG"
                     /locus_tag="ACV5NJ_000288"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HHY6484418.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13711..15072)
                     /gene="murD"
                     /locus_tag="ACV5NJ_000289"
     CDS             complement(13711..15072)
                     /gene="murD"
                     /locus_tag="ACV5NJ_000289"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HHY6484419.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15086..16048)
                     /gene="mraY"
                     /locus_tag="ACV5NJ_000290"
     CDS             complement(15086..16048)
                     /gene="mraY"
                     /locus_tag="ACV5NJ_000290"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HHY6484420.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16074..18266)
                     /locus_tag="ACV5NJ_000291"
     CDS             complement(16074..18266)
                     /locus_tag="ACV5NJ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HHY6484421.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18267..18668)
                     /gene="ftsL"
                     /locus_tag="ACV5NJ_000292"
     CDS             complement(18267..18668)
                     /gene="ftsL"
                     /locus_tag="ACV5NJ_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HHY6484422.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18673..19632)
                     /gene="rsmH"
                     /locus_tag="ACV5NJ_000293"
     CDS             complement(18673..19632)
                     /gene="rsmH"
                     /locus_tag="ACV5NJ_000293"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HHY6484423.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19653..20084)
                     /gene="mraZ"
                     /locus_tag="ACV5NJ_000294"
     CDS             complement(19653..20084)
                     /gene="mraZ"
                     /locus_tag="ACV5NJ_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HHY6484424.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20254..20622)
                     /locus_tag="ACV5NJ_000295"
     CDS             complement(20254..20622)
                     /locus_tag="ACV5NJ_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HHY6484425.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20793..21749
                     /locus_tag="ACV5NJ_000296"
     CDS             20793..21749
                     /locus_tag="ACV5NJ_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HHY6484426.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21822..21938
                     /locus_tag="ACV5NJ_000297"
     CDS             21822..21938
                     /locus_tag="ACV5NJ_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HHY6484427.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21993..23675)
                     /gene="recN"
                     /locus_tag="ACV5NJ_000298"
     CDS             complement(21993..23675)
                     /gene="recN"
                     /locus_tag="ACV5NJ_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HHY6484428.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23693..24142)
                     /locus_tag="ACV5NJ_000299"
     CDS             complement(23693..24142)
                     /locus_tag="ACV5NJ_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HHY6484429.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24249..25067)
                     /locus_tag="ACV5NJ_000300"
     CDS             complement(24249..25067)
                     /locus_tag="ACV5NJ_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HHY6484430.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25076..25966)
                     /locus_tag="ACV5NJ_000301"
     CDS             complement(25076..25966)
                     /locus_tag="ACV5NJ_000301"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HHY6484431.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25968..26192)
                     /locus_tag="ACV5NJ_000302"
     CDS             complement(25968..26192)
                     /locus_tag="ACV5NJ_000302"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HHY6484432.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26197..27534)
                     /gene="xseA"
                     /locus_tag="ACV5NJ_000303"
     CDS             complement(26197..27534)
                     /gene="xseA"
                     /locus_tag="ACV5NJ_000303"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HHY6484433.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27535..28389)
                     /locus_tag="ACV5NJ_000304"
     CDS             complement(27535..28389)
                     /locus_tag="ACV5NJ_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HHY6484434.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28486..28935)
                     /gene="nusB"
                     /locus_tag="ACV5NJ_000305"
     CDS             complement(28486..28935)
                     /gene="nusB"
                     /locus_tag="ACV5NJ_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HHY6484435.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28928..29353)
                     /locus_tag="ACV5NJ_000306"
     CDS             complement(28928..29353)
                     /locus_tag="ACV5NJ_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HHY6484436.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29599..30663)
                     /locus_tag="ACV5NJ_000307"
     CDS             complement(29599..30663)
                     /locus_tag="ACV5NJ_000307"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HHY6484437.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30835..31080)
                     /locus_tag="ACV5NJ_000308"
     CDS             complement(30835..31080)
                     /locus_tag="ACV5NJ_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HHY6484438.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31219..31593)
                     /locus_tag="ACV5NJ_000309"
     CDS             complement(31219..31593)
                     /locus_tag="ACV5NJ_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HHY6484439.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31676..32590)
                     /locus_tag="ACV5NJ_000310"
     CDS             complement(31676..32590)
                     /locus_tag="ACV5NJ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHY6484440.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32611..33414)
                     /locus_tag="ACV5NJ_000311"
     CDS             complement(32611..33414)
                     /locus_tag="ACV5NJ_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HHY6484441.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33447..34436)
                     /locus_tag="ACV5NJ_000312"
     CDS             complement(33447..34436)
                     /locus_tag="ACV5NJ_000312"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HHY6484442.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34539..35030)
                     /locus_tag="ACV5NJ_000313"
     CDS             complement(34539..35030)
                     /locus_tag="ACV5NJ_000313"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HHY6484443.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35221..38028)
                     /locus_tag="ACV5NJ_000314"
     CDS             complement(35221..38028)
                     /locus_tag="ACV5NJ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HHY6484444.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38462..38755)
                     /gene="rpmA"
                     /locus_tag="ACV5NJ_000315"
     CDS             complement(38462..38755)
                     /gene="rpmA"
                     /locus_tag="ACV5NJ_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HHY6484445.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38768..39112)
                     /locus_tag="ACV5NJ_000316"
     CDS             complement(38768..39112)
                     /locus_tag="ACV5NJ_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HHY6484446.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39139..39447)
                     /gene="rplU"
                     /locus_tag="ACV5NJ_000317"
     CDS             complement(39139..39447)
                     /gene="rplU"
                     /locus_tag="ACV5NJ_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HHY6484447.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39467..39543)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39729..40370
                     /locus_tag="ACV5NJ_000318"
     CDS             39729..40370
                     /locus_tag="ACV5NJ_000318"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HHY6484448.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40432..42063)
                     /locus_tag="ACV5NJ_000319"
     CDS             complement(40432..42063)
                     /locus_tag="ACV5NJ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HHY6484449.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT        11854 a         9165 c         7155 g        13905 t
ORIGIN      
        1 aacgaaagaa tgagtcttct ctatcatctt tcgttttttg ttgtaaaaac gtctaaaaat
       61 cctttgtttt ttatttagaa aaatagtcaa ttaacgtttt ttttgttaca cttactaatg
      121 atggtttttt accatgaaag gatgatcgaa tatgacacca aatcgagaag attatttgaa
      181 aatcatttta gaactaggtg gagatacaac gaaagtcaac aataaacaaa tcgtatctag
      241 tctagatgtc tcagctgcat ctgtcagtga aatgatttct aaacttgtga aagaagagct
      301 tgtagaacat tccccttatc aaggtgtaca attgactgca agcggactgc aaaaagctag
      361 cagtctgatt cgcaaacatc gcttatggga agttttttta gttgaacatt tagactatag
      421 ctggaatgaa gtccatgatg atgctgaagt attggaacat gtgacttcgg aacatcttgc
      481 agatcattta gaaaactatt tgaatcatcc tgaacattgt cctcatggag gatcgattcc
      541 taaaaaagag gaagttgtcc atgaagaacg ccgacaaaca ttggagtcct atcctgtcgg
      601 tacaaaagtg cgtatcgcac gagtgcttga tgaaaaagaa ttgttggatt acttagtcac
      661 tattgacctg aatatcaacg aagaatatga gatcgttgac atcgcagctt atgaaggacc
      721 gatcactatc caaaatgata gaaagaaaat ccccgtcagc tttaaggcgg ccagcacgat
      781 ttttgtcgat aaaatataaa ggagtgtatc cacatgtcta atgaaaaaaa tgtattgttt
      841 acgatattcg gcggtacagg tgacctagcc cagcgtaaat tgtacccgtc tttgttccgc
      901 ctttacagaa aaggaaattt aggcgagcat tttgctgtaa tcggaaccgc tcgccgtccc
      961 tggagcgatg agcactaccg tgaagttgtc aaagaaacaa tccgtgcatt gaatccttca
     1021 gatgaagaag caactacctt tgcaagccat ttttactatc aatcccatga tgtaaatgat
     1081 tcaacgcatt atcagacatt aaaagaattg tctgaccgat tagatggaca ctatcaatta
     1141 aacggtaacc gtgtctatta tttggctatg gcacctcaat tttttggaac gatcgtttct
     1201 catttgaaat cacaaaatat tatgacaaaa aatggcttca atcgtttgat catagaaaaa
     1261 ccgttcggtt ctgattacca gtctgctttt gaattgaacc agcaaatcag agaagtattc
     1321 ccagaacaag atattttcag gattgatcat tatttaggaa aagaaatgat ccaaaacatc
     1381 tcagctatcc gttttgccaa taatattttt gaggctcaat ggaacaatcg ctatatcgac
     1441 aatatccaaa ttacttttgg cgaaaacctc ggtgtagaag atcgcggggg ctactacgat
     1501 catagtggcg cacttaaaga catggtgcaa aatcatattt tacaggttgt agcattactt
     1561 gcaatggaac cgccagtagc attttccgaa aaagaaatcc gtacagaaaa gatcaaagca
     1621 ttgaaagcta tccgcattta taacgaagaa gaagtgttag aaaacttcgt tcgcgggcaa
     1681 tatgctcaag gtgaattgga tggtgtacaa ttcaagggct atcgcgaaga agacaaagtg
     1741 gatgctcaat cagagactga aacattcgtc gcaggcaaat tcactatcga taacttccgt
     1801 tggtcaggtg ttcctttcta tgtacgtaca ggcaaacgtt tgactgaaaa agggactcgg
     1861 atcaacatcg tattcaaaca agttcctgtt aatgtattca aaacatctgt cgatgaacca
     1921 tgtgacgata cgacacttcc acctaatgtt ctaacgatct atattcagcc aactgaagga
     1981 ttttctttat ccttgaatgg gaaagaagta gggcaaggat ttgaaacgga accaatcaaa
     2041 ttggaattta gaaatagtgc agaaatggtt gaaaatagcc cagaagctta cgaaaaatta
     2101 ctattagatg cgttgaatgg tgatggaacg aacttctctc attgggaaga agtcgctcaa
     2161 tcttggcata tcgtggatgt cattcgaaaa gcttgggacc aaacacaacc ggattttccc
     2221 aactacaaag ctggatcaat gggacctaaa gcagctttcg aactacttga aaaagacgga
     2281 ttcgaatgga tctggcaacc agataactgg tatcgtgaac gtggaaaatt aaacgattga
     2341 taacaaaagg gggtgtgaca aagaacatgt cacaccccct tttccctttt acttttcttc
     2401 gaatccttca gctacagctt caggatagaa gtcttcaacg atttttgcac aacgaccgca
     2461 tagatgagga agtttttcat ctactcctac atctttacga acttggcgac agcgatcgca
     2521 agtttccccg tctgcttttt ccactgtaat agcaacatca tcaaatacca tagcgttatc
     2581 tggcacttgt tcatctgctg ccataacttc aaagtagtct ggtgaaacga tcaataattg
     2641 tgcaatatct gcatccaaag cttttaacat ttcacgaact tgttgttttg gataaacagt
     2701 tagttttgct tccaaagatt taccaatcaa tttgctgttt cttgcttctt ccaatgcttt
     2761 taagacatta tcacggaagt ccatgaaagc agtccaggta tccatcaatt cttcttggtt
     2821 tgcaaactct tcataaccag gaaactcgct taactgaacg tactcctctt cttctttcag
     2881 gaatgaccag atttcttctg ctgtatgcgg aatgatagga gtcaataatt ttgtcaaagc
     2941 tacagctgtt tgatagaaaa cagtttgcat gcaacgacgt tgataatcat cttctgcttc
     3001 aatataaact acatctttag caaaatccag gtagaatgac gatagatcaa ccgtcaagaa
     3061 gttcataact gtacggtaaa tatgcatgaa gttatatttg tcgtaccctt cttctcggat
     3121 ttctttgatt acttggttca atcgaacggt catatatttg tctacagaac gtaaatcatt
     3181 gtacgaaaca gtatcttttt taggatcgaa atcttctgta ttcgctaata agaaacgcat
     3241 cgtatttctg attttacgat atacttctgc cacttgactt aagatatcca tagaaacacg
     3301 aacatctgct tcataatcta cactagatac ccataagcga aggatatctg caccaaattg
     3361 tttgatcact ttttctggtg caatgatgtt tcctaaagat ttactcatct tacggccttc
     3421 accatctaaa gcaaatcctt gagaaataac tgatttgtat ggtgctacac cattgatagc
     3481 cacactagtc gtgatacttg agttgaacca gccacgatat tggtctgatc cttctaaata
     3541 catatctgct gggaatgata attctggtcg ctgacgtaaa acagcttcat gagaagaacc
     3601 agaatcaaac cagacatcca tgatatcttt ttcttttgta aattcgccat taggagaacc
     3661 tgggtgggta aatccttcag gaagaagttc tttcgcttct ttttcaaacc agataattga
     3721 tccatgttca gcaaataatt ttgccacatg ttcgattgtt tctggtgtga tgattgcttc
     3781 tccattttca gcatagaaaa tcggaagtgg tactccccat gcacgctgac gggaaattac
     3841 ccaatcgccg cggtcacgga tcatattata caaacgtgtt ttaccccatg ggataaccca
     3901 ctctactttt tctacttcat ccaaaatatt ctgacggaat ttatcgatag atgcaaacca
     3961 ttgtggagtt gcccgataga tcacaggctt tttcgtccgc caatcgtgag gataactatg
     4021 agtaaagaaa tctagcttca gcaaagctcc tttttctttt aatagatcag tgatcatagg
     4081 atttgctttg tcataaaaga taccttcaaa tccaggtgct tcttctgtaa acaatccttt
     4141 tgagtctaca ggtgacacta cagatagttt atatttgcta ccagcgatat agtcatcttc
     4201 cccatgtcct ggagcagtat ggactaatcc agtaccagcg tctaaagtaa catgatcgcc
     4261 taaaatcaca agtgattcac gttcatagaa tggatgactt gcagtcatat attccatttc
     4321 ttgtccagca aattctttta gaactgttac attttcccaa ccgatttctg attggactgt
     4381 ttctagcaaa tctttcgcta cgacaaactt acggccatcg gcttccacca atacatacgt
     4441 aaattttgga tttacagcaa taccttggtt tgctggcaat gtccatggtg ttgtcgtcca
     4501 aatgacaaaa gctgtatctt catcaagaat atctttgccg tctttgacat tgaaagctac
     4561 aaaaatagat ggagatttta catctttata ttcaatttca gcttccgcta aagaagactc
     4621 acttgacgga gaccagtaaa ttggtttcaa tcctttgtaa atatagcctt tttcagccat
     4681 tttaccaaat acgcgtactt gtgcagcttc ataagaagga tctaatgtaa cataaggatg
     4741 ttcccaatct cctgcaactc ctaggcgttt gaaatctgct cgttgcttat ctacttggga
     4801 aagtgcatat tcacgacatt tttccaagta ttctgccatg gacatttctt tacgtttcac
     4861 gcctttatta gccaatactt gttcaatcgg caatccatgt gtatcccatc ctggaacata
     4921 tggtgcacga tagccagaca ttgattttga acgaatgata atgtctttac tgattttatt
     4981 caatgaatgt cctaaatgga tattaccatt ggcatatgga ggtccatcat gaagaacaaa
     5041 gctaggtttt ccttcgttca actcttgtct tttttggtag attcgatttt cttcccattc
     5101 tttttgccac tcgccttctc ggtttggcaa attaccacgc atagggaatt ttgtttttcc
     5161 taattgcaag gtttctttca ttttcattca accatacact cctttatttg ttttccggac
     5221 acaaaaaagc atgttcagcc gcaagggacg aacatgccgt ggtaccaccc aaatttagtt
     5281 gcatctatct agtgcaaact ctctgtgacc aataacgagg tcagccgctg cttcttactc
     5341 taatttcgga aaagcaggtc tcaagtagag gatctttcac catgcagtgt cttaccgaac
     5401 ttccactctc atcggctcgc tttaagaata acaaagtgct tttctgttct actcgttttc
     5461 attttcacca ggcacgatcg agatatcaat agcctgtgta tcaaatgccg tcataggtgc
     5521 ttctatgact cctgtgtctg agtttacatc agtatcattt tcattgtcaa gctgttttgc
     5581 taaaacttct ttgatcactt catggctgtc agtcacgtag ctggagaatg gttgtaaaat
     5641 ttcatcccat tccggacttt tcacttgttc taattgagac tcaagcatca agctgatacg
     5701 ttgatggaat actcgtgttt ttttcttaag atcttcagtt tctgtcgcaa gctgtcttgc
     5761 tttttctgtt gcatctgtca agatttttct tgctttttct tctgcatcag ttgttaattg
     5821 atgtgcacgt ttttcagcgt tcgcaactaa ctcatctgct ttgttttgtg cagaagtaac
     5881 gatcacttcg gattccttgc ttgcacttgt ttttacttta tcagccgtat cttgagctac
     5941 gatgattgat tggttcaaag catcttttaa ttcattgaag tactcaagtt tttcttcaga
     6001 atgtttgact gctttttcta attctcgatt tctttgagtc aattcctcat agtcacgaac
     6061 aactaaatct aagaaatcat ctacttcgtc ttgattatat ccccgcattt ttgtcgggaa
     6121 tgtcttattt tggatatcta atggagttaa agccatgagt aaaccacctt tttattttat
     6181 ttacgtaata caccgaacag taccctgtac ttttcttttt tcgtcttccc ctcaagttct
     6241 tggatctgga ctctgccata acctctgact gaaataatat ctaatagttc aagtacaaaa
     6301 tcaggtcttg tattttctgt ccagttcact tttactttac cagcttcgat cagctgcttg
     6361 gaccgctgtc tggaaatatt ataaatctca gagatcacac tgtctagtcg catggaacta
     6421 actgttcctc tttcttgtgt ccagctatct ttcggcataa tcatttctgt gtatttgatt
     6481 ggttccaagc gtacagcaac ctttccaatc tttgttactt gtgcagcaac aaaatgatcc
     6541 acctcatttg caataaatac ttgccagcga tcaccgtccg agatgatatc gccaaagtaa
     6601 gagcgtttga tccccgttcc cactaaagtt ccaaggattt tcccatgact taggctggca
     6661 aatttgctag gataatggat ttcatacaat gctgtttcaa aatcttcctc tacaggttcg
     6721 taataatcgg ggaaaatcaa acatcttttt ctttctgcct gttcatatcc gccataaaac
     6781 atgaaacgca aatcactgct ttgtcgaatc agtgtttcta aaatatagga ttgtcttggg
     6841 tcaagaaatt cggtcagata gggtgcatat tgtccttgaa cctgttcaat ccaatcgcct
     6901 accgaatcga taaatgtacg ttcatctgcc cgaaagtgct ggtatacatt tgcatccaaa
     6961 attttcccct cctatctatt ctgagaataa agattttgtt gcttcaatca ataaactaaa
     7021 tggacagtta tccatataac gatctgtgct aaaccttgta cggctaagtt caagatcagg
     7081 attgccccta aaattgtgaa atccaagggt ccaatcctca aaggcagacg atcgaacaaa
     7141 ctgatatacg gttcgcagat cttagctaat aatcgtccta gccctgattg atacccgcct
     7201 ggaaaccaag agagcaatgc atataccatc aagataatgc tatagaaata ggctgcccgt
     7261 tgaagtaaat gaagtaatag tgcaatagta taaatcgtag ttcctcctct tacagatcaa
     7321 acaaattcgc atctgcaaaa gattgagcgg ttgaactgtc aatctccatc ccagtcggtg
     7381 tacaaagaaa aatctcatct ccaacacgtt tgatatctcc atctagtgca taaactgttc
     7441 ctgttagaaa atcaacgatt cttctcgctt gtttttcttc tactaaatga aaattcacta
     7501 aggttgcttc tccagcaatg attcgtttag caatcgccat agcttcagaa taagctcttg
     7561 gttccattac agtgattttt ccagcaagat ttgctttagg ttgtgtagag gttcttgaag
     7621 ctgttctttc tttcgctaca gtttttgtag gtgattgatg tagttcaatt acttttttct
     7681 tttcagccgg ctgaatactt tcatgcgtta caggacgtga agtcgccgtt ttttgttttt
     7741 catgagtatt cgcagcagat acagaatgag ttgattgatt ctgtgttgta ttttttttag
     7801 ttggctgtac agcctttttt gttgtttcag gataatcttg gtattcgtac tcttcctcat
     7861 cagctaatcc gaaaaaatta gcaattgctc ctctgtttaa aagcggcatt tttgttcctc
     7921 ctttattcgt ctttgaatag cgctgttcct acacggataa atgttgcacc ttcttcaatc
     7981 gcaatttgaa agtcttgact catccccata cttaattcac tacatggtgc atgggcataa
     8041 ccttttgctt gaacaagatc acgcaattct ttcagcgtac caaacacttt atggatttgc
     8101 tcatcagaag cttctttagg cgccatagtc atcaacccaa caaccttgat ttttgtgcag
     8161 gcagctaaag attccacaaa cggtaaaagc tcatctggtg aaattccatg ttttgattcc
     8221 tcaccagaaa catttacttc aataaaacag gataattctt tctctaaccg cttctggatt
     8281 tcttctgcta atcttaaact gtccaacgca tgaaagtaat caatttcatt tacaatcaat
     8341 tttacctttc tgcgctgtaa attaccaatt aaatgccact ttatttcagg aaaatcaaat
     8401 aaatcttgtt tcttttctaa taatttatct actcggtttt ctgcacaatt cataacgcca
     8461 gctctaacca attcttcagc ttgagcggat tcaacagatt ttgttacagc tactaatgta
     8521 acttctttcg gatcacgatg aacaagagca cacgaatcct gaatttcttg cttgattttc
     8581 tgcaagttgt cagcaatcat gtgctcttcc tctcctttat tttttacgac ggaaaaacgg
     8641 tggcgtattt aattcatcat cgccatgaga ttttgtttct tcgcggttga atgtatcaaa
     8701 ttcttttttt tcgatattgt caaaattgga atcatcaact ctaggacgaa cattttgttc
     8761 acgtctgata tcccaatcgc caaaactgtt ttcttcttct gaagaagttg gtttagcttg
     8821 atccatatct aatacagtct tttgagcagg tgattgcatt tgtgcttgtc tagctggtcg
     8881 gctagacttg cgttcctttt ttgattcatc gatacctgtt gcgatgaccg tcacacggat
     8941 ttcatcgccc atttcttcat tgatagatgt acctagaatg atatttacat cgccagttgc
     9001 tgcatttgct acgatatctg aagcatcttg tgcttcgaac aatgtcatat ccaaaccgcc
     9061 tgtgatgtta agaagtactt gttcagctcc atcaatagat gtttcaagca atggagagga
     9121 gattgctttt ttcgttgctt ctactacgcg atcttctcca ctagccacac cgatccccat
     9181 caatgctgta ccttggtttt ccatcactgt tttcacgtca gcaaagtcaa ggttaacgta
     9241 acctggcgct gtgatcaaat cagagatacc ttgtacacct tgtcgtaaaa cattatctgc
     9301 ttcacggaaa gcttcaagca tcggtgtctt tttatctaca acttctaata aacggttatt
     9361 tgagataatt aataatgtat ccacattttc tttcaaacga gcgatacctt ctgcagcaaa
     9421 acggccacgt ttcggtcctt caaaagtgaa tggacgtgtc acgacaccaa cagtcaatgc
     9481 gcccaattct ttcgcgatac ctgcaacgat tggtgcagca cctgtaccgg ttcctccacc
     9541 cattcctgca gtgatgaaaa tcatatctgc accgtctaaa gcttcgcgta aggattgttc
     9601 actttcttct gccgcttttt gtccaacttc tggctgagaa ccagcaccta aaccacgagt
     9661 atatttgggg cctaattgga taactgtttc agcttttgaa ttttttaatg cttgcacgtc
     9721 tgtgttggct gtaatgaatt caacaccttt aacgttttct tcaatcattc ggtttacagc
     9781 atttccaccg ccaccaccga ctccgatgac tttgattact gcgccgtcat tgatattatt
     9841 atcaattgag aattccatat cttattcctc ccaggatgaa ttaatcaaaa atatttgtaa
     9901 agaaaccctt gattttattt tttacatttt ctccggactc ttttggttcc ggctcatcgt
     9961 agattgtttc ttcaggtgct tcatagctct catatgtgtt tgttgtttct tgttcaacta
    10021 ccatttgacg agctgctgag gtttcacccg taacagcaat ttttgccaat tgatacactt
    10081 cactcaagtt tgctgaataa tccacgatac ttattacatt tgtaaatacc gggtttctca
    10141 gccccatgtg atttggaaca tacaatttta catttacgcc gaagatttct tgtgccaagt
    10201 ccacaacacc aggaagacta gcagctccgc ctgtcaatac gacgccacct ggcaactcta
    10261 atgcttcgat ttggtccaat gcatcttttg ctttgttgaa aatctgttcc attcgagctg
    10321 aaataatttc tgacaagtag cgttcatcga ctttcactgg ttctgactga ccaatcacat
    10381 ctactggaaa ttcttcatct ggtgatgtcc gttctgggta tgcatccccg tagtttattt
    10441 tcaacgcttc tgcattgtta aatgacgtat ttaatacaat agagatatct ttagtgacaa
    10501 attcgccacc ttcttggtca agattggtaa acttcaattg cttatcatgc ataacagccg
    10561 ttgttgtttg gccgccaccc atatcaatga cgatcgtacc aaaatctttt tctccgtcag
    10621 aaagaattga ttcagtcagc gctaaaggag taatcaccat ttcgttgacg attaatccgg
    10681 cattttccac gcatttacgt atgttatgga tgatcgtttt aggtcctgta aacaacagac
    10741 cgtacatttc taatcttacg ccgatcattc cacgaggatc tttgattccc tcgaacccat
    10801 cgacagtaaa atcttgtggc aatattgaca cgatttgacg ttcaggaggt attgaacgaa
    10861 caagggcagc tgaagccaca tttctgacat cttcatcggt gatttccttt gaatctccgt
    10921 taactgcaat cattccctgg caattttcta cttcaagcat gtttgctggc aaaccgacac
    10981 ttacgccttt gatctggata cctgcttttt cttctgcttg tcgtactgct cgttgtatcg
    11041 cttggaccgt tttatcgata tcaacgataa tgcctcggtt aatcccttct gattttgcgt
    11101 ttcctactcc aataatattc atttggctgt cgatatattc agccacgaca actttaacag
    11161 atgtcgttcc aatatcaagg cctacataca ttcctgtttt tgccatgaat gggattccct
    11221 cctacttact attccatata caagcgaaca actagcgtcc gttttaaaat atacttatta
    11281 atagatatat tcactgcttc caattctacc ataaattact ggtttttaga aagtacattc
    11341 ttacttttta aagttgtttt atcaattttc atttaaactt gacggagttg tacttggatt
    11401 atcttctatt tgacttgttt cacttgaatc atttgaatca gatgatccgt ttgtgctaga
    11461 cgatccaggt gtttgtgctt tttgcgtttc tgcctcttca cttgattcgt ttgtttgatc
    11521 cgcattttct tcactttcag ttgtttcttc cgattggccc gattcttccg tttcttgagt
    11581 gctaccgtaa ggatgcgaga agataccaac ttccatgtcg atcacacctt tttcatccat
    11641 atctttggct acttgcgaat aatatttcat ttggttcgca agattattga tatttacaat
    11701 aacttgattg ccgtcattca tattcaattg gatcagattt ttattgcttg ctcttggcgt
    11761 gtacttgatt tcagaaattg ctttttgtaa ttctgatgat aactgattgt attgctttgt
    11821 taattctttg atttttgcag agtctttgaa ctcttccaag atagggagat tttttgtagg
    11881 atttgctact ttttcatcta atactgttcc attttcaatc actgggtagt attgattgtt
    11941 ttttgcgatc aaagcaatct ctttatattc tgttacatcc aaatcgaatt cattcatccc
    12001 tttgaaatgt atatttgctg tttcgattct tggctgtgct tttttaaggc gatcgatata
    12061 agtagatcga tgaaaatact gtccccaaac attttctcct aatgacaatt ttgtatcaga
    12121 gatgatttct tggctgttga ctgtcttatt gcctgatact gcaactgctt gtaatttgct
    12181 ataaggagaa acataataga tcaaaaaaat cagcggtata cccaatacag tgatgatcaa
    12241 agctaaccga cgaaatagta ctttgtgccg ttgacttttt aaattgggca gccggttcaa
    12301 aaaagaaccg ttatatggtc caccgttttt cttttgcttg atctcttcaa aaaattcgat
    12361 ttttttctcg gactggcttt cttcttgctg ttcctcttga accgtttttt cttcttcaga
    12421 atctgaaagg ggtgtatcta atgttatgtc ttcttttttt ggctcttggt ttttcatctc
    12481 ttgttctgcc agatattttc ggttagcctt ttgccaagga gtcaaattgt tttcatcaga
    12541 agacggatga gacaattttt ttggttcttt tttcttactc actaagacca ccgcctttat
    12601 tttttgatta tttgttttac taagtcataa agacgttttg aagcatcagg gattccttgt
    12661 tctttagaag ctcggcacat ttgcttttgc aattcttcat cacccatgat ttcgttgact
    12721 gtctgactta gtgattcacc tgttagttca ttatctgcaa tcatttttgc tgctcctgcg
    12781 tgaacaagac tcattgcatt tttagtctga tgatcattcg taacatatgg gctgggaacc
    12841 aacactgccg gtaaccccag agctgtgaat tctgcgatcg aagttgcacc agcacgcccc
    12901 accagcagat cactggaagc catgacctct gccatatttt tgatataagg ctggacacta
    12961 atattttgta tttcaggatt tattttattt aggatctctt cataatatct atctcctgaa
    13021 gcatacaaaa cttggtaaga agaaggaata tattcactta aaaaaactga tactgcttga
    13081 ttgattttaa gtgctccttg actaccgcca aaaatcaaga ctgttttctt ttctggatcc
    13141 aaatcaaatg ttttcaaaat atctgatttt ttagtatctg ctacttcttg tgctcgagga
    13201 ttacctacaa gaactgcttt gttttcagga aaaaaggaag ccgcatcttt aaatgaaaga
    13261 gcgatccgat ccacatatcg gcttaaaaat ttattggtaa cacctggtac actgttttgt
    13321 tcatggataa ctgttggaat acccattttg gctgcagcgt acactaccgc tccggaaaca
    13381 tatcctccag taccgataac gatatctggt tgaaaatcac tgaggatttt ttttgcttga
    13441 cggatactct taagaaacaa tcggaccgtc ttgatgttat gcatggaaat ttttcgttta
    13501 aatccttgaa tctccaaggt atggaaaggc attcctgtat cagggacgat tttgttttct
    13561 agacctcgtt ttgctcccac atacatgaat tcagcattcg gttcttgagt ctttacataa
    13621 ttgacaaagg caagagctgg ataaatatgt ccccctgttc ctccgcctgt aaccaaaatt
    13681 ttcatttttt atcgctcgct tctacttttt ctattttaat cggttcacag cttccatgaa
    13741 tctgtttcct ctagtttcaa aatttgggta ttgatcccag ctggcattcg ctggtgataa
    13801 taaaactaca tcaccatctg tactgttttc taatgctaaa ggaactgctt gttctacaga
    13861 atcggctgtt actattgtct cgatgcctgc tttttttcca gcatccatca acttttcttt
    13921 tgtttggcca aatacgacca ttgctttgat tccttttaat gaaggaatta attcatcaaa
    13981 tgtatttcct ctgtctaatc cgccagccaa taacacgacc ttagacgttt caaatccact
    14041 tagagccatt ttagtcgcta aaatattggt tgcttttgaa tcattataaa attttctgcc
    14101 ttggatttct cctacatatt gcgtacgatg tggtacgcca tggaaaaaat gcaacgtttc
    14161 tcgtatagct tcatttgata taccatacaa ttttgctaca gaaatagcag ctagtgcatt
    14221 ttctacatta tgcttacctg gtacgccaag ttcagatatt tccattattt tttctttttt
    14281 atagtatatt gagtaatcgt cagcatacac accgtcttcc aacacttctt ttgtcgagaa
    14341 aggcagtacg cgtgcttttg ttctttgcgc taattcttgt agttctgact gattccagtt
    14401 aaggatcaga taatcttttt ctgtcatgtt tttttgaagg ttccatttag ctttgacata
    14461 ttcttttctt gtaccatgat aatcaatatg tgcttcatag atattggtaa tcaccgctat
    14521 atgagggcgg aaatctgtga ttcccatgag ttggaagcta gacaattcca tcacaatttt
    14581 atcttctgat tttgcttctt gcgctacacc acttgctgga tacccaatat tacccgctaa
    14641 acgtgcaatt ccttgatctg ctcctgcatt cagtagtagt cctgtcatcg ttgttgtcgt
    14701 tgtttttccg ttcgttcctg taatcccaat aattgggcat tccgctactt cgtaagctag
    14761 ctcaacttcc gtaattaccg gaatacccat ttcttgggct tttgctacta aaggatgact
    14821 gtagggaatc cctggatttt tgaccatcaa tgaaaattct tcatccaaca attcgattgg
    14881 atgactacct gttattactt ttatccctaa agaaagcagc tcttgtgctt ccggattttc
    14941 gtcaaaaggt tttccatcat taacggtgac aagtgcgccc aattcatgta ataatttggc
    15001 cgcacttact ccgctttttg cgagtcctaa gacaagcact ttcttatttt catatgttga
    15061 tatttttttc atttcccgtc atctcctacc aaacgatcca taatgtgatc aacgaagtca
    15121 caatgctgat caaccaaaaa actgtatcaa tcttccactc cgaccatccg cacatttcaa
    15181 agtgatgatg aattggagac attttaaaaa ttcgttttcc tgtaagctta aaggaagtaa
    15241 cttgaagcat cacacttgct gtttccataa catagatcaa accaatcaaa agcaatgtcc
    15301 attcttggtt tagcattata gaaattgccg ctaaaagtcc gcccaacgct aatgaaccga
    15361 catctcccat aaaaatcttg gcaggttttc tgttgtagac gaagaaaccc agtaaacctc
    15421 ctaaaacact taagcaaatg attaatacat catactgatg ctgatgccaa gcaattagac
    15481 cgtaagctgc aaaagagata cttcctaagc cagcaactaa tccatcgatg ccatctgtca
    15541 gatttactgc atttgaaaat ccaacaagcc agaagagagc gaaaatacca taaatgattc
    15601 ctaaaggtag atctagtccg aagaaattca atacacctgg ataaccttca gaacggtaaa
    15661 caagataaaa gatgattccc cctagaatct gtccaatcaa tttctgcttc gagttcagtc
    15721 ccatgtttct tttcttgaat atttttataa aatcatcaag aaagccgatt actccgtata
    15781 acgctaatac aaaaagcaag atgaaaagag taggagttag ctgtttttgc caagctccaa
    15841 cccaaattcc tgtcaaaata gagccgatca gaaacactaa gccgcccatt gtaggagttc
    15901 cggctttgct attgtgccac tttggtccct cctcacgaat cgcctgacct tgctttttca
    15961 tttgaaaata accaataaat agcggcattg cggcaattgt cattgcacaa ctgctgacaa
    16021 tgggaatcaa tgcttgtgtc cattccatgc tcttttctct cctatttctc ttcttattct
    16081 tctaaagtaa aactaagttt gtcttcagtg attttttcat atggagcaag ttcttgtttg
    16141 acacaataac catctccatc aaaactaact tcaataccga ggattttccc cagcttcatg
    16201 atatctgctt ttgaccatcc tgttacgtca ggcatcagct tatcaccacc agtatatagg
    16261 atcagttttt ctccagagat caactgatca ccgttggcag tagattgttt ttgtactttt
    16321 ttaccattac cgatcactac aggttgcagt ccactttttt gagcatcagc tgctgcaacg
    16381 tccgcttcaa gattacgata atcggctacg gatacttttt ctgtttttgt ttctgtatct
    16441 gaatcttctt cagtttcttt aaaatccatt gcccgtttca tcaatggatt tgcgatttta
    16501 gccaatgcca ttcgatcata tgtcttaggg tgtttcattg ttagatacaa gacatagtct
    16561 ggatcttcag aaggaaccat ctccacaatc gaatacaggt aggctgtatc tcctgtttga
    16621 tatccccctg tatcagaagc gatttgggct gttccgtttt ttgccgatac attgtatcca
    16681 ggtacactat atacaccata agcactaccg tagttttcgc tttcgacaac atctctcata
    16741 tattcacgga ctttttctgt tgtttctttt gaaaaaggtt gccctagaac ttctgtttga
    16801 gttgttcgct cttctccagt ttggggatcc accactttgc tgatatatcg aggttgaatc
    16861 attgtaccgt tattcgcaat agaagtgaaa gctttcatca tttggaaatt cgtcacacct
    16921 accgcttgtc cataagcact cattgctcgg tcgacaatat ttaatgtagg taaagcaccg
    16981 tttacttcat catctaatcc agaatgagta ctttgtccaa atcccagttt ttgtaaatag
    17041 ttataccatc gtccgcctaa tcgctgttcc agtatgacca tcccgacgtt acttgaccat
    17101 gaaagagctt gacgcatggt cagcacacct ttttcaccaa agtcatggtc attgatcgta
    17161 gcatcttcta cttggatttt tccagattgg aaggtttcat tctcgttgaa ttctccttct
    17221 tcgatagcag ctgcagtagt gaaaactttc atggttgatc ctggttcata actatcttgt
    17281 accaagaaat tacgccagat agcatctttt ccagtcaatc cttccattgt ttcaggattg
    17341 aaagtaggtc gttgtcccat tgccaagatt tctccggttt ttgctttcat caggactgct
    17401 gtcaattctt ctggctgata ttcttcattt acttgatcca tcagtgtttc taaataactt
    17461 tgtagtctgc tgtctaaagt cgtgtagata tcttgtccgt cgacagcttt ttcttcttct
    17521 gccactgtac ctggcagagg attttgaaaa ttatcttttt gataaataat cttaccatct
    17581 ttaccgctca agatatcatt gtaagcagac tccaaaccta gtttaccaac taagccattc
    17641 tcatctttgt ctggaactgc gtatccaata aaatgagaag aaaagacgcc atttggatac
    17701 atccgagcct gatggtcgac aaaataaaga ccagcgacac cttctttttt catgtccgct
    17761 tcgatgtttt gcttggtttc ttgggagata tttttcccat agctaccaaa ttcaacttga
    17821 tacagctctt tttttgcgcc gtcctctaaa atattcagtg catctttttt gtctatagaa
    17881 agattcttat gcaggatttc tgctatttta tcaaaattct tttcttcaac atacagtttt
    17941 ttatccccag aggtatatgt ttttgagagt atcgctttaa ttgaataaga ggaggcatct
    18001 tctgcgagcg ccacaccgtt acgatcataa atcgttcctc tttttgcttt tactacttca
    18061 cttccttggt acaactgttt tgttttttca gctaatgagg taccagcaac atgacctcca
    18121 ataacgatat acgagaaacg gacggcaaat aaaaagaaca atccaatact cgtagcaaat
    18181 aaaatgatac ctactttttt gcgattgttc atcggattta gatttttctt ttccataaac
    18241 cgccgaattt tatttttaag actcatttac ttcactttcc ttaaattgtc gtcattaata
    18301 gacaatcctt tttcttctgc gatttgttta atccgttcag tacgagaaag ttcgtttttt
    18361 tcttgttcta aactcgtttt ttcttgcttt ttatcattga tttctgtttg tattgacgtg
    18421 atttcttttt cagctccgct gatatttgtg cgcagcataa ctgttaaaac acataaagca
    18481 acaatagcaa tcgccacgct tgccacagca attttttcca aagcagaaat ctgctttaat
    18541 cgttttgccg gtgattgtgg aagaatgatt tcttgattcg aagaagaatc aggcatttgt
    18601 tgttccggct gatccaccaa ttcaggtaaa tcatattcgt aatcttgcat tttctttaat
    18661 tctgccatca ttctactcct ctttcattct tattttttct gcaatacgta actttgcact
    18721 gcgtgatcga ttgttttctg ttagctcttc ctctgaagga aggattggtt tacgagtaat
    18781 gactttcagt tctggttgga attcgtcagg tatgacagga agtcccggtg gtagatcttg
    18841 taccgtacta tattctttaa acatactttt gacaattctg tcttccaatg aatggaatgt
    18901 gattacgctg attcttccat tgactttcaa taaccggatt gcctgttcta aagaggcctc
    18961 ttcggcaccg agttcgtcat ttactgcaat ccgcacagct tgaaaaattc tttttgcagg
    19021 atgcccgcct tttcttcttg cgggagctgg gatgacttgt ttaatgattt cgacaagctc
    19081 accagttgtt tggattggtt ttgtttttcg tactcgttcg atttcacgag caatttgttt
    19141 ggaaaatttc tcctcgccat agcggaagaa aatctttact aattcatggt aggaatattc
    19201 attaattacg tgataagcgg ataaaggagc tgactgatcc atgcgcatat ccaacggtgc
    19261 atcttgatga taactaaatc cacgttctgc ttcatctagc tgaggagagg aaacccctag
    19321 atcatacaaa atgccgtcta ccttatctac atgctcactc aagcgttctt ctaattctct
    19381 gaagttcgct ttgataaatg tgaccatacc ttgttcgaca taagaagcta aacgcagctt
    19441 ggcgtgatcg attgcttttt gatcttggtc aaacgcatac agatgccctt tagaacttag
    19501 ttggcttaat aaatattcac tatgtccggc accgccgagt gtacagtcta cataaacgcc
    19561 atccggtttg atgttcaatc catctaccgt ttctttcaac atgactgtat agtgttgaaa
    19621 tgtttcagtc attagtcagc ctcttcctat tcttatagtc cgaaatcaat catactttca
    19681 gcaatttcgt caaagttctc ttctgctgct gcagaaaaat cttgccatcg tgtctcatcc
    19741 caaatttcaa ttcggttaga tacaccgatg actacgcaac cttttgtcaa agctgcataa
    19801 tttcttaacg tgctgggtat attgattcgt ccttgtttat caagttcgca ttcagtggca
    19861 gccgaataga agaatcgcac aaaagtacgt gcgtcttttt tagccaacgg catttcgttc
    19921 agtttagctt ccagattttc ccattcgctc atcggatagc cgaaaagaca gccatccaat
    19981 ccgcgtgtca gaacaaactt ttcgccaagt tcttcacgaa gttttgaggg cacgattaaa
    20041 cgtccttttg catcaatatt atgttggaat tcacccatca acattgaaag ccccccattc
    20101 ctccactaac taaaattagt ctaccacaat cccccacttt ctaccacctt ttctgggaaa
    20161 tccgggaaca acttttttta ttcataaaca tttttttaaa aatcaagctg caaaaagcct
    20221 tttaaaagac tttttacagc ttttctattt ttttcatagg taagaaagta tgttcacaat
    20281 gatcaagaca gcgtacaaga cgattgtcaa cagaaaaacc aaacgccaag tcatcttgaa
    20341 ataacgcgga taaataattt cttcataata atatgcttgg aaaacagcaa tacaaattcc
    20401 cattagcaaa acagaaataa agaaaaacgg taagatagaa attgtccctg tatttctaga
    20461 caatgcatgc ataccgcccc acaaaaaagg aatcgccaaa tcaggggctt ttattttaaa
    20521 acgacgattt aacgaaaata attttacaag aaattggcaa ccaaacaaaa cgattaccgg
    20581 gtaaatatac caaaacaaga taagtggtgt gaatgtgccc atgaacgtaa actccttttt
    20641 tatcgttgct tcaagtatac tgtatatact ttacacaact gtttcatttt atttaaattt
    20701 atgcagttat attctatatc ttacaaacac attccatgta ttccaattcc ctatccgtta
    20761 taataaaaga aacaggcaac aggaggaaaa tcatgatcaa atattttaca ttagataatg
    20821 agaaactgcg taataccgga aaggaaactg accaaacgat ctggtattgt gtagaacgtc
    20881 ctaatcaaga agagattcat cagctgacac atcaatttga aattccgtca gattacatca
    20941 cttccatatt ggacgatgca gaaaattccc gtacagaaga attcaatcaa tcaaagttga
    21001 caaagccagc gttattattg ttacaatatc cttttgctaa aacaagtcct agcggttatt
    21061 ttcaagtgga tacatacccg ctctctatta ttttgacgcc caaaaagaag cttatcacaa
    21121 ttacgaatca tgaaccactg tttttaaaga aagtctttag ccagaccttt ccaaaaagtg
    21181 atatttcgcc taatctcaat atcttctttc aattcatttg gcaggtcaca ctctcttaca
    21241 acagttattt agctacgctt caatctcaat gcgatacttt ggaagccaaa cttcaagtat
    21301 ccactgaaaa caaacaactt tatcaaatca tggatatcca aaaaagcctc gtttatttca
    21361 gagaagcgac ttctgctaat ttagatgcat tgaaagaact agcgcaaaat aaacttttcc
    21421 aagaaaatca tgctttgaaa aatcatttac gtgatgtatt ggtggaaaca gagcaggcaa
    21481 tgactacttc tcgcattcaa ttaaaactag tagaacaaat gaatcagact ttttcagcaa
    21541 tcgtatcgaa taatctgaat aatattatga aaattttaac ttctttaacg attattttaa
    21601 ctataccgac aatcgtcggc agtatctatg gaatgaacat taagttacct attgctgaaa
    21661 gggatgatgc ctttatatgg ctagttctat taacattatt tatttgtatc gtaactacct
    21721 attatttaaa aaaagggaag tttttataaa aaagccctct tgtggaaatg gaaaaaagat
    21781 tgtacacttg aatcaactaa ttaatgacga ggttgaaaac aatgcgtgaa aaaaaacaaa
    21841 caagtacttt tgctaaagta acaaaatttg tcatttggac aatgctgatt ttaacgattg
    21901 gatctgttgt attgactgct gtaatgagtg tcatgtaaaa ataaaatata gctatcagct
    21961 gctgaaaagg acctgaacca ctgcgaggtg gctcaggtcc tttttgcgag tttcaatagt
    22021 tcttttgcat gttcgagtgt gagttttgta atctcacttc cagccagcat acgtgcaatt
    22081 tcttcttctc tttcttttgt tttcaactct gaaacagaag tttgcgtacg tccatcaatc
    22141 actgctttca caatataata ttgatagtcg gcgactgcag caacttgagg cagatgagtg
    22201 atgcacaaaa cttgagagta tttcgatatt tgagaaattt tatctgcgat ggcctgtgca
    22261 actcttccgc tcacacctgt atccacttca tcaaaaataa tacttgtcac accttgctca
    22321 gaagaaaaaa tagttttcaa agcaagcaac atccgagaca gttcgccacc agaagcgact
    22381 ttcaccaaag gcttcagtgg ctctcctgga ttggtcgtaa tgtaaaattc gatttggtca
    22441 aatccatcat gattcaattg tctattttct tcttttaaga aacgtacctc aaactcagtg
    22501 ttttccatat acagactttt caattcatgc agaatggatt tttccaattc ttttgcgatt
    22561 tttttacgcg cttcatgaag atctgcggct tgttgctgaa gtagaagctc tttttgctct
    22621 agttcctctt tcattttgtc caattgacct tctgaaaaat cagaagaagc tagctcttcg
    22681 gtgatttcgt catagtaagc caatattgac tcaatggatt cgccgtattt acgtttcaac
    22741 tgtcggatta attctaaacg ttgcgtaact tcttctaaac gactctcatc taactccaat
    22801 aaatcaatct gacgactcat atcgccgatt gcatcttgca gcaaatagta agcactttgg
    22861 atattttcat aaattgcttc atactccaca tctagatggg cgatcccttg cagttctgaa
    22921 acggccaagc caacaccatc tacactgttt tgttcattat tcgtcagtgc tccgtaacct
    22981 gcagctaatc catcagcaat tttttgataa ttgctaagtt tttctctttc ttcttttaat
    23041 ttctcttctt ctccgacttg caaatcagca gcagctattt cttcttgctg gaaatggagc
    23101 atatctattc tctgaacata ggatttttca ttttgctgca cttttcgtac ccttttttcc
    23161 agctcacgat aagagatata ttcttgctga taactctttt tcttctgttg gaaagctaca
    23221 tctccaaaac gatccaataa agcaagatgt gattcaggct ggagcagttc ctgatgttcg
    23281 ttttgtcctt ggatatctac taaataactg ccgattcttc tcagattagc taatgtgata
    23341 atatgaccat tcactcgaca aacatttttt ccagttaagg acatatcccg tcgaacaatc
    23401 agattgtcct catctgtttc gatccctaat tcaaccatca gttcagagaa tccttcttgt
    23461 tttggtagtt caaacaaccc ttcaagcaca catttttctg ctccttgacg tatataatcg
    23521 cttgaacctc ttcctcctgc taacagacct aaagcatcga taataatgga tttacccgca
    23581 ccagtctctc cggtcagcgc ggtcattcct tcgtgaaaag aaagccgcaa ttccggaata
    23641 atcgcaaagt tagtaatact gatttcttgt agcatcacat tcgctcctca acttaaaggt
    23701 aatgcaggaa gtctttcttc aataaaactg cctggtcttc agttcgtgta atcatcaaaa
    23761 cattatcgtc atcattgata atcgaaaata attctttctt gtaacgttta tcaatcaaat
    23821 ttgctgctgc cgaagcattt cccggcaatg ttttcaaaat aacaaatttt tccatttggt
    23881 caactgccac gaaagcgtct tttaatagtt tttccaattt agtatttaca tcttcgtttg
    23941 tttcagctgg tatgctataa cggtagccgc catctgaagc cggtaccttg atcagtttca
    24001 gttcctttat atctctggaa atcgtagctt gagtaacaga tattcctttg cttttcagga
    24061 tttcaacaaa ttcttcctgc tttcggatat cttgctcact caacaatcga gtaatcaaac
    24121 gatgccgatc tttttttctc attacgtctt cgctccttag catattcttc ttaatcaatg
    24181 atagcttatt tccttggtat aataaagaag aatccgcata cttctcactt gattttcaca
    24241 gttacaattt atttatggtc aaactgttcg tgtgcatttg aaaccacttc tgccatatta
    24301 atagctgaat tgattgttcc gctttcgggt actttcctca agtgagccaa gaactcgata
    24361 tttccttctc cccccgtgat cggagaataa cttaattcca gcacatcata tccaccttgt
    24421 gccgcaaatg acaaaatgtc ttctaaaact ttttgatggg tctgagggtc acgaacaatt
    24481 ccgtttttcc ctacagcttc tctgcctgct tcgaattggg gtttgattaa agcaatcact
    24541 tctccctctt cttttaaaat agcatgtaat ggaggcagca tcagttttag tgaaataaaa
    24601 gaaacatcga taacagcaac ttctggaatt cctaaggtaa agtcttcggg ttttgcataa
    24661 cgaaaattca ctctttccat tacgacgact ctaggatctt gtcttatttt ccaagctagc
    24721 tgattgtagc caacgtctaa agcataactc atttttgcgc cattttgcaa agctgcatca
    24781 gtaaatccgc cagtggatgc accaatatct aacaatgttt ttccttcaac agatagatca
    24841 aatgctttga gtgctttttc taattttaat ccccctcgag aaacataagg aagtttttta
    24901 cctttgactc ttaattcact actcactgga attttttctc ccggtttatc aaaccgctcg
    24961 tttttttcgt tgtaaatcaa accagccatc actgaacgtt tggcttgttc tcttgtttca
    25021 aataatcctt gtttgaccgc taatacatct acgcgttctt tttccattct ttccctcatt
    25081 tctccacatc aaattgcttg atcatctggc gcatcagccc gccatcaaaa gaaaaaacgt
    25141 cctcttctaa tttgtctatg atcttgttgg cggataacag ctcaatctcc aacgcctcac
    25201 gtgtcttttc taggcctaat aaacgcgggt aagtattttt ttccatacgt tcatctttac
    25261 cagctgtttt ccctaatgtt ttcgttgtac caatcacatc gagtagatca tctcttatct
    25321 gaaaagctaa acctaaatgt tcggctaatc gttgaagtag caataagatt tcttcaggtt
    25381 gcttcgctaa tatgccacca gccagcaagg cataacgaat caaatgccct gtctttcttt
    25441 catgaataaa tgctaattct tctaaagaaa gctttttcga ttcgccctcg atatctgcag
    25501 cttgcccagc aaccatacct tgtgatcccg cacaaacagc taattgctga agcagcagaa
    25561 gcttaggaga attacctaag tgtgccatac tgattagctg gaatgctcct gtaagcaaac
    25621 catctccggc caatatagct aatgcttcgc cgaatacttt atgattggta ggttttcctc
    25681 tacgaagatc atcattgtcc atcgcaggca aatcatcgtg gatcaaggaa tacgtatgca
    25741 ccatttctaa agcagctgct acttgataga cgggaacatc aataggctca tcaaaggaag
    25801 caacggccgt taaaagtaat aagggacgta ttcttttccc gcctgctttc actgaataac
    25861 tcatcgcatc atagagtcct ctatccgttg tatgctcatc taaaaaagac aagatttctt
    25921 tttctacagc aggtagatga atagctgaaa agtcagttaa tctcatctca gctttcctcg
    25981 tttccgtcaa aatcaacttc ttcatttgat tctgtcatca ttttcgtcaa ggtctcttct
    26041 gctttagcta atgtatcttg gcaatgtttg cttaattcca ttcctcgttt aaacgaatct
    26101 agagctgatt ctaatggaac gtctccttgt tctaactgca tgacaatttt ttctaattct
    26161 tgcagtgact cttcaaaagt tggattttcc atatttttat tctcctttgt ctatctcttg
    26221 tacttgtcct ttaacgattc catcttgata atggatcgtc agcaaatccc cttgatgaat
    26281 ctcggtgaca ctttttacta cttgatcatt taacgtcgta tagctgtagc ctcttcccat
    26341 gattttcaat ggactcaaca aatcaagaga ttgaacagct tgagcaaaac gctgctgctg
    26401 ttgtttcata tattgttgga ttttctcttc taagcgtttt tctagataag cagtttcttg
    26461 gcgagctgct ttaactcgat aaagcggagc cacttgttcc aattggtggc ttaatgcgat
    26521 cgtttgtttt tctttcgcat gataaatttg ctgtgtagct tgatataatc gctgtttcaa
    26581 ctggtctaat ttgatggtct gaccttcata caatctttca ggctgccgaa atacatagga
    26641 acgtcgagca cgctcgaacc gttcttgttt gcgctggatc tggcgtacaa acgcctgctg
    26701 caatctagct tgccgttctt gaatgcgcat caactcttct gacaaaacag gaacagctaa
    26761 ttctgcagct gctgttggag tggcagcccg aacatctgct actaaatcag cgatcgtggt
    26821 atctgtttca tgtcctacag atgaaatcac cggcgtatta gcttgataaa tcgcccgagc
    26881 aacccgctct tcattaaacg gccataaatc ctcaatcgaa ccacccccac gaccgatgat
    26941 cattgtatcg aaattgccca tctcttctac tctctggata ttcctgacaa cattgtccgc
    27001 tgcttggttt ccttgtacaa ctgttgggaa caaaaccaat tggacaattg gatatcttct
    27061 tttcaccgtc gtgatgatat cgcggataac cgctccgctt ggactggtaa ggaccgcgat
    27121 tcgctttggg aaacgtggca gcattttttt aggcccttga aaaaggcctt ccttcatcag
    27181 cttttctttt cgttcttcca attcttgata taaagcacct attccatcag gctccatgtg
    27241 ctctacatag atttgataag aacctcccgc ttcatataat gaaatacgcc caacgacaag
    27301 gactttcatt ccttcttttg gctggaatcg taatttttgg aatgctcctt tgaacataat
    27361 agcagatatt tttgcatggt catcttttaa gctgaaatat tgatgggcat tggcacgtaa
    27421 acggaaattc gatatttcgc ctgttaaata tacgcgttct aaataaggat cggcatcaaa
    27481 ttttcttttc aaatacttcg ttaatgtagt gacagttaaa tattcttgtg ccatctattc
    27541 acctgctcca aaagcttcgg cagattttac ggtttgatac atcagcatcg tgatagtcat
    27601 tggccctaca ccctttggca caggagtgat ccagcctgca ataggctcca cttcatcaaa
    27661 tttcacatca ccgatcagct taccgttagc atcacgattc atccctacat ctatcactac
    27721 agcacctggt tttacgaatt cttttgtgac aaaatgtcca cgtcctattg ctacgactaa
    27781 aatatctgct tcttgtgcca gttcagccag attttcggtt ttagaatgag caacagtaac
    27841 agttgcatct gccatcaaga gtagctgagc catcggttta ccgacgatat tgcttcgtcc
    27901 gataactact gctcgtttac ccgccggatc gatagaataa gcttcaaaca ttttcatgat
    27961 gccgtatgga gtacaaggaa tcttgtccgg tttacccgca aggagtctgc ctaaattcat
    28021 cggatgaaaa ccgtctacgt ctttttcagg gcggatagct aacaggatct tttcttcatc
    28081 tatatgcttt ggcaatggta attggaccaa tataccatga aaactaggat cttgattgta
    28141 tttttcaatt tctgcaagta gttcgtcttc agaaatcgtc tcaggataac gctctaccaa
    28201 tgaatggatt ccgatttctt ttgctacacg ttctttattt cttacataga tttggctggc
    28261 tggattttcc cctaccaata aaacgactaa tccgggatgg ataccattat ctttcaattt
    28321 ttggattttt tcagcaatct ctgcctgcat tttttcagct aattcttttc cgttaatcaa
    28381 ttctgccatc taaccactcc cagatatttt ttgtgttttc attctaccat agaattttcc
    28441 cgatttataa caggtttatg atgattcata gaaaaaagct aggacttacg ccccagcttc
    28501 taattctttc aatacattgg ataaaacacc gttgacgaat ttacgtgaac ggtcatcact
    28561 aaatgttttt gccaactcga ttgcttcatt taaagcaaca gccgctggta catctgtcac
    28621 ataagtcatt tcaaaaatcg cgatacgcaa gatcaccaaa tccatttttg agatccgatt
    28681 gatgtgccaa ttgtttttta aatgtttttc aatcacttta tccaattcat ctttttttgc
    28741 acagacaccg ccaactagcg tatcaagata aacaggaaca aactctgatt catcttcgtt
    28801 gatcatgtcg cggtggtcta attcgattgc gttgaaaatc gcatcttctt ttgttaggtc
    28861 tgcattgaag tctaatggaa ataaagcctg caatgccatt tcccgaatct cgtgtcttga
    28921 taattcttta ttcattctct tcctcttcgt cttcaaaaag ttcttcgatt gttggttgtt
    28981 ctaatttttc aggaacaaca gctactacat ggatattgac ttcagataaa tcgatatccg
    29041 tcatgaaaag gacttgctgg ctgacccgtt cctgcatttc cattgctact ttaggaactg
    29101 aaacaccata ttctagataa cagtaaagat ctactttcaa gccatcttcc tcgtttgcta
    29161 agtaaacacc ttttccgtga gctgcacgtc ctaataattc agtgacattg ctggcaaaag
    29221 ttccgcgcat tccgtatacg ccttctactt tagaagctgc aatcccgata attacttcga
    29281 tgacttcagg agcgatcacg atctctccta aatcttggtt tgcgtttaaa atcaaatttt
    29341 tttcatctgc catttattta ttcctccctt tgcctaagca cgagatacat ccgaattctc
    29401 tatgatgcgg catagtgatc acgtggtaag aaaactgccg ccgatcagct gcaaactgct
    29461 atgaattgga gaaaacaccc aactttatta tctcttacag cttaaaagtt cttctatagt
    29521 gtatcattaa atcgagtgat tttctataac aaagtctgtg aacactgatt tttacgctta
    29581 cttcttctat tgatgaaact ataagatgat caattctttt ggtgaacgag tcaacacacg
    29641 atttccatct tctgtgatca acaagtcatc ctcaatacgt actccaccga ttcctggtaa
    29701 gtagatccct ggttcatcag tgatcacatt tcctggtaca aattgctttt ctgcacgaga
    29761 agaaacgttt ggtccttcgt ggatctctaa accgatccca tgtcctgtag aatgtccaaa
    29821 tgcttccccg taaccatgtt ttgaaatata atctctagca actgcatcta attggacacc
    29881 agtcattcct ggttttgcag catcaataac tgctaattga gcatctaaga ctatttgata
    29941 aatttctttt aatttttccc caggatcacc aatagcgaat gtacgtgtca tatcggaaac
    30001 gtacccttcg taatagcaac caaagtccaa tgtgatcaag tcaccttgtt cgatgatttt
    30061 tttgcttgct acaccatgcg gcatagccga acgcaatccg ctagcaacga tcgtatcaaa
    30121 agaaacacct gaagcgccaa gagaacgcat ataaaagtct agttgatttg cgacttcaat
    30181 ctcagtcatg cccggttgga tcattttcag gatgtgatca tatgccatat ccgcaatatg
    30241 acatgctttt tcgataatag cgatttcttc ttcatctttc acttctctga gttcttcgat
    30301 catctgtgaa acaggtacta attccgcatc gatgatttct tctaaaaccg aatattctaa
    30361 aaatgagact gttgtttcct caaaagctaa tgagtgcagt tcttctttgc gtactaagtt
    30421 tgctacctca tcaaaaatag gtccgacatt tttaataatt tcaaaccctt gagcctgcgc
    30481 tgccgcctgc tcggtataac gaaaatcagt aataaaaaat gctttttcca aagtaataac
    30541 agctaatcct gtcgtacctg taaagttcgt taagtaacga agattgtacg gactggtaac
    30601 caaaaatgaa tccaaatttt cttcttgcat ttttttgcgc aatttttcta cacgtagcat
    30661 cataatgaaa ctccctcttt ctttgcacgc ttttttccat tatagcaaac ttttcttcgt
    30721 acacgacagt taaataaaaa tttccggaaa acttcttttt attttaaata aataaagcca
    30781 aaaaggagtc acagtctcgc tgcaactcct tttagtttac tattttgtaa aaacttatga
    30841 tcgttttcca aagaaaaatg agacaactgc taccaaaatc actgcgccta aaatagatgg
    30901 aataagtgcc atacctgcag cttttggtcc ccattcgccg aaaatcgctt gtccaatact
    30961 tgaaccgact agtccagcaa tgatattcgt tacacagccc atcgaatttc ctttactggt
    31021 gatcgcacct gcaattgctc cgatcacggc tccaacaatc aatgtccata accaactcat
    31081 aatataacct ccttctttgc taatcaattt tagcatatgg gatttaaagg ggacaaataa
    31141 ttggttttgc tatttcatat tttcttctgc aaagtaaaaa aataaagaaa acctcctctt
    31201 agaagttttc tttatttttt acttgcgttt aaacatacta cgaataccac gtttcactac
    31261 atcaaagaaa ctcaatgatt gtaccattct atcttcatct acatattctc tgatcgcacg
    31321 aagcactttt ttaggttctt tggaagaaaa tgtgtatgta ccattttttt tcgtttgaat
    31381 ggcatatcga ggaatccatt ttccctttag catgactgat gctagtacat aatccacttc
    31441 ttcccatgga atttggatat atttgcgtct atctcgcgca tggtaaaact caaaaccatt
    31501 attacctatc atgatttgcc cgtaatctgt cagacccgta aacgaagtag catcaattac
    31561 caagtctact tttgtattga ttgattgaac cattttctcc ctccagatat ttgtttatga
    31621 acagtatact atataaaaaa agctcgggac aacgcccgag ctatattttt atggattata
    31681 aaagtccgat gacgtgtcca acaacaccga ctacgaataa accaaggatg ataacgattg
    31741 gagaaacctt tttcttaagc aaccacatac ataacaatgt taatcctaat gcagctaaac
    31801 caggaatcaa ttggtctaag ttgtcttgta atgttgttac cttttcagga gaaagggcta
    31861 agccattatt aacttgttca aatgcactct tgattccttc accacctgaa ggcaatttat
    31921 cccattcgat gtaagcacct ttatctaatt ttacagttga tacaactggt aagaatttga
    31981 ttgataccca tctttgtaca agggcagcta atacgaacat ccctaaaatt gaagcacctt
    32041 ttgttacgtc ttgtaacaat ccgccagaaa ggtcatcagt aattttagaa ccagctttgt
    32101 agccaaattc ttgtgtatac cacatgaatg cccaacgaat aatgttccaa cctaagaaga
    32161 agatgattgg tccaaggata tttcctccca tagctaatga agcacctagt gcacctagca
    32221 ttggacgtac tgtaaaccag aacactgggt caccaacacc agctaaagga cccatcatac
    32281 ctactttaac cccttgaatc gctacatcat caacaggcgc gccattcgca cgttcttctt
    32341 ccagagccaa tgtaacccct aaaattggag aagcaatgta tgggtgtgta ttaaagaatt
    32401 ccaagtgacg ttttaatgct gcagaacgtt cttcttttgt tttgtataat tttttgattg
    32461 ctggaatcat agaaaatgcc cagccaccgt tttgcatacg ttcgtagttc catgaacctt
    32521 gaatgaaggt agagcgccac gcaacagcta aacgatcttt ttttgataat ttgatttttt
    32581 cttctgccat tttattttcc tccttcaaga ttaatagtca ttcaaaatgt cgcctaaagg
    32641 gtcaccagag ttgcttccgc ctccattatt tgatgaacca cccattttag aaaggttcaa
    32701 gtaaataaga gctaatgcaa cacctaaagc acctagagca atcaatgtta attgagaaat
    32761 tgcagctaca acgaaaccaa taacgaagaa tggccatact tcttttgtag ccatcatgtt
    32821 gattaccaaa gcgtaaccta cagctacgac cattccccca ccgatagcca taccatctgt
    32881 taaccatgct ggcattgatt caaggaatga ttgcacagtt tgtgctggga taaatagcaa
    32941 cgctgctgct gggaccgcga tacgaacccc ttgtaaacat acagcaatga tgtgccacat
    33001 ttcaacctta cggatgtttc cttcttcagc agccgcatcc atcaaatgaa cgattggaac
    33061 agcgattgta cgtacgatca ttgttaagaa caaccctgca acagccaaag gaacggcaat
    33121 cgcgatcgca gaaggaacac ctttaacacc ttgtccacct aaaactaaaa taattgctga
    33181 tgctactgat gctaatgcag catctggtgc tacagcggca ccgatatttg cccaacctaa
    33241 agcgatcatc tgaagcgtac cgccaagtac gataccagct tcgaggttac cggtaactaa
    33301 accgatcaat gtacatgcca ctagtggttg atgaaattga aattcatcaa gaataccttc
    33361 cataccagct agaaaggcaa ctaatacgac taaaattatt gaaataatag acatgataag
    33421 cctcctataa attttaatat tatttattac gcttttgcta attcagcttt tgcttttttc
    33481 aaaatatcat ccatgttatc gcgtgaatca ttaggtactt tacgtacatc gaatttcaca
    33541 cctttttgtt caagcttctc aaacgcttca acgtcttctt gtcccataga caatacttta
    33601 cttacaacga ctttaccgac cgaatgcgcc attgacccaa catttaattc tttgatgtct
    33661 acgccacctt caacagctgt caatacgtct tcaggatttt caaataaaag caatgctttg
    33721 gtatttccaa aacgagggtc ttttgctact tcgatcattt tgctaatagg aataacattt
    33781 gcttttacac ctggaggagc agcttgttcg attagtctct tacgcagatc atcctttgat
    33841 actgcatctg aaacaacgat aatacggttt ggttgcactg cttttgtcca agctgtcgca
    33901 acttgtccat gtaacagacg tgagtcaaca cgtgctaata catatttgat ttttccgtct
    33961 ccgaccacag ttccttctgg aagtgctcct ttaggttgtg cgtcttcaac tgctgctttc
    34021 ggtgcttctg ccggttctaa ttcttccggt tttactttta caccgtcttt tgctgtttca
    34081 atgatgtgtg ctgcaatttc atgagcagat tccattgaaa agcgtgatgc gtaggcttca
    34141 atcaacattg gtaaattcaa accgctgacg attgcccatt tatctttgtg ctcttcgaat
    34201 aatgtgtttg attgattgaa cggtgttccg ccccataaat caactaagaa taacacttcg
    34261 tcttgattat caaaagaagc aaccgcttct tccaatttgg ctctcaagtc atctggacct
    34321 tcacttggtt tcaaaatcac ggctttgacg ttttcttgtt cgccaaagat cattgaaccg
    34381 gattgtaaga ttccttcagc aaattgtcca tgacttgcta ggataatccc taccatattt
    34441 atacctccta tttacttttg ttgtaacaaa ttcaaaaata ttaataattg ctaaccatta
    34501 caacagaatt atttgacctt ttccaacaac cattcttgct acaccttttt gattttcgct
    34561 agtagttcca tcaaaaggat tttcctgtca gcaggtactt ttcgaatctc taactccact
    34621 tcttgactgt tcaagtaacg aaaggaatca atatcttgtt gatttactga aacaaaattg
    34681 gtaatcattg atttgccttc agtaaaactc ataccgccga tattcaacga cttcaactgt
    34741 acaccgcagc ggacaaccgc ttcaacatct tggggattgg tgaataaaag catcactttt
    34801 gctcgtgata ataatccact atgatagatt tgaatcattt tctgcacggt tattacattt
    34861 gctgtgatcc ccggcggtgc agcttctttt agtagaaact ttctcaattt atcttgagcg
    34921 actacatcgc tgatcacaat gatacgttca acacctgttt gcttcgacca aactgttgcg
    34981 acttgcccgt ggattaaacg atcatctata cgtgccaatc gaatatccat ctgcaaaact
    35041 ccttcttcct cactacttca cagaaacccc tatcatttct gtgtgaacaa ccacctccta
    35101 gcacacacta ctataagcaa aaagcgtgcc aactttttta cgatacacta attgaattta
    35161 aaggattctt aaccaataca caaaaacgat acactataaa agtgtatcgt ttttgtgtat
    35221 tcatggcaca gtgtattctt ttttatctaa ctgattatgg actagacgta gcagataata
    35281 catttcttct atcggcactt ttacttttat ctgatcttcc aacagaacaa ctgaggctgc
    35341 caattggcta taaagaggat cattcgttgt atttgtcaat tcgttttctt ccacagtcaa
    35401 tggttcattc cgaataatac gttcgatcat tcctgctgtg tgcatcacta ggttgatttt
    35461 gaatccatat tcttcatcgc cgattcccgt cgtctgagag atttgtgtag aaaattgcca
    35521 cattggatcg attaacttac taccattaat aaagatgaag ttatcagaga taaactcaac
    35581 acacgtattt ttagctgact cttcatctag ggagatgaat tcagtttcct ccaattctga
    35641 ttccatcagc aactgatcca aaatgacttc gatattttga tcaataaaac gttctaaagg
    35701 aataaatggg gcttggatat tcaaatcagt aatcccagta gtagctatga tctgatattc
    35761 tttttggata actggcagtt cttctttcac ttccacaatc gacaaagcta gaacttctaa
    35821 ttctgtttct tgacgcttgt ttaccgctcg ttcgatcaat tctttgattc tttgtgctgt
    35881 accttcgcca gatgcgcaga tagccaaaac agcttttttc ttatgaatag gttttgcttc
    35941 ctgaacagat tctacagaag atatttctgc gtaacctcga aaatttttca atgattcatg
    36001 caacatatct agatctgtcc ctaatactga gacctttcgt acagtttcca aaactagaga
    36061 agttgttacc atatcaacag tacgtactgg aattccagtg tctctttgaa tttgcgaatt
    36121 aaacgacgca agggacccca tatctactaa gagtatgacc ccgcttcctt catctacttc
    36181 ttgtacattg cgttcaattc ggtccaatgc tgttttcgga tccatatcca aaggcatgtc
    36241 tactgctcgt acgttctctg catctaacaa ctgttggaca acttggacca tactgcttgc
    36301 tgtgctgttt ccgtgagctg caacgactac accgattctg ccgcttgctt gatttgcccg
    36361 taacgagacg agtagaacgg tcaagtaata gtcttcactt tctggaatct ccacttgaaa
    36421 atacgtttcg atataactac gcacttcttt cgcaacagca tattcatctg gaaaatcaaa
    36481 agccatctca cggatgttat cattcgtatg ccgttcttct cctaagttga tttttttcaa
    36541 aaaagaactg atatgcaagc tcatagcata aataaagttt tgctgaaact catatggtaa
    36601 agatcttttg accattacat aaatctgatt ggtcacttct atgattttcg gatctacaaa
    36661 ttctgctaat ttgctgtctg cattaaaaga aaaaccatgg tctttataga aagacttcag
    36721 atggatgttg atatctgtag aaatgaaatg attgattgct tcttgatcca aaccatccga
    36781 cttcagtaaa gctgctttat ctccgatgat atcatataga ttataaggta actcatagga
    36841 gtccgattgg attttcatga tcgtatcatt tggtgctaca gttatctttg gctccaatat
    36901 tttagaaagc tctgccattg cactgcggtt gcttgctaat tgaatcaatc cgctacgaat
    36961 cccttctgtc aaatcatcga ttgtgatcga gatttcgggt gattgcatat gatttagaaa
    37021 tcctcgtgca catactaact ggatatttga cttcaattgt cctatatttc cataggtcac
    37081 actgccgatc aatgcttttg ctacatcttc tgtcaaagaa atctttcgct gcgtgcggtt
    37141 tgcttcgtgg gcaatcatga tttttactaa atcgactttc tcatttgcag gacgtttttc
    37201 aaaagccgga agtttgatat tgattgggat acgccgaaca aaagtttcta atagagaaga
    37261 acctgggtct tctgtggtcg cacctacgat ccgtacattt gcttcatggg atttcgttgt
    37321 ttccccaaga cgactgtaag ttccatgatc cataaagtaa aagatcattt cttgaccttc
    37381 aggcggcaac cggtgaatct catccaaaaa cagcataccg ccatccgctt gatcgataat
    37441 ccccattttt tcttcatctg cgcctgtaaa agcacctttt gcgtacccaa ataaatgact
    37501 catcaaaagc tcagggttat tggcataatc agcacaattg aaaacaatca gttccgtttc
    37561 ctcatctatt acatgatgcg cttttgcaaa atgatacatc gcgtgtgcaa aataagtctt
    37621 tcctgatccg gtaggtcctg taatcaaaca atttaatcct cttggcggat acaaaatcgc
    37681 agcttttgcc tgttccactg aatttttcat gctgccgtta gctccaatga tttttgcaaa
    37741 gatatcctta gtatctattt ggagtgtatt tttttcttca gagaccggcg tttctttgta
    37801 actgactaca tgttttgtaa ctggctttac gtgcgtaacg agtgtttgat atatgtaacg
    37861 tactggtcgt ccgtctgtct ttatcagttt tccttcgcgc actagctgat tcagatcttt
    37921 actgctattt gttcgctgaa tccctaacac ttcagcaatt tcctgtgttg ttactccttg
    37981 atcatattct gcctttgtta aatgagcggt tttttctttg acatacatat aaattcggtc
    38041 aatgcgtttt gccaatggta tcgctccttt tctatagact attgtactta ggcttcaaaa
    38101 cataatacta tgtatttatt tctgtttcgt caactattta cctgattttg ttttgttgat
    38161 aacacgcgga tagatagaaa tgttcaattt aaaagaacga aaaatcccga tacgattttt
    38221 ataatggaaa caagaaaaag aacactgttt accatataat tatttcactg atagcaaaca
    38281 atatagcttc catcaattga aaacgatagt tatcaaaaca tcctgattcc aaattggcaa
    38341 aaaaatatta agtgtaaaaa gacaaagtaa acttcgtagc ctccactaca acttttattt
    38401 ttataaacaa aaaaggctgt gacaaaagtc ttgtcacaac ctctttaatc ataagattca
    38461 attaagcttc ttgagctact ggataaacag acacttgttt tttgttacgg cctttacgtt
    38521 cgaaacgtac tacgccatca acttttgcaa ataaagtgtc gtctccaccg atacccacgt
    38581 ttacacctgg atagattttt gtaccgcgtt gacggtaaag aattgatcca cctgtaactg
    38641 tttgtccatc agcacgttta gcacctagac gttttgattc tgagtcacgg ccgttagatg
    38701 tagaaccgcc accttttttg tgggcaaata attgtagatt catagataac aacatggtct
    38761 gcacctccta tttatcatta attgttttaa tttggatata ttctgagttt tcagcttcta
    38821 tcgattgtaa tcctaataaa agattttcta atagaatctc agcaatattg ttttgttctt
    38881 gtgtcattcc tgaagtgact tcggtataaa gataaccgcc ttcttcttca ttcatttcaa
    38941 caatcggctc aaaaccggct aatgaggcaa tcccgttgac agtgctgatc gctaacgcag
    39001 aaacacccgc acaaacgata tcacttccat aaggaccagc atcagcatgc ccggtcaaag
    39061 tgaatgaaac gatctgacct gcgtcatttc gcttaaatgt tcctttgatc atatataggc
    39121 cttctttctc aaacgctatt aagcgttgat cgcgtcgata accacttttg tatatggttg
    39181 acgatgacct tgtttacggt gtgaatgttt tttaggtttg tatttgaaag taacgacttt
    39241 cttttgtttg ccgtgttttt caacagtacc ttcaacagtt gcacctgcaa cagttggagc
    39301 gcctactttt gtagattctc cgcctactaa gataacttcg tcaaaaacaa ctttttctcc
    39361 tgcttcaacg tcaagttttt caacatagat tgcttgtccc acttcaactt ttacttgttt
    39421 accaccagtt ttaataattg cgtacatgct ataaagcacc tccttctgat atacttagac
    39481 tcgccatccg aagtgacagc atatgctgtg cttattactt gttctgagcg gttgtagctg
    39541 tggagtccac aattacaaca gtactagctt accagaacag caaccgttag tcaactaaat
    39601 ttaaaatcaa aaagtaaaac aaaaattcaa gcaatacttc tgcttttcta tcctcacagt
    39661 tctaccaaat aaaatagtcg cattgtgttt cttttattct tataataaaa gaaaaaggag
    39721 gagcataaat ggaataccca aaagaactga tacaagaagc ttctttaaga atgaacggcc
    39781 gtcctgtaga aggatttatc gctgggcaag gacctcttca tcctaaactg atgctcgtgg
    39841 gagaagcccc agggaaaaca gagattgata cgcatgttcc ttttagcggt caagctggaa
    39901 aagaattgat gcaggcactt tcttcaactg gcttaacacg agaagaggtg tatatcacca
    39961 gtgctgtaag gagccgcccg tatcgcgtga ctcatcggat caataaacgg acacaacagt
    40021 cagaaactgt ttatccaaat cggacaccta ctcgttccga agtatttgcg catgcaccaa
    40081 tattggatta cgaactgatg aaggtccagc caaaattaat tgcgacactt ggaaacattg
    40141 gtcttcaacg tttacttggc aaagaatata cggttaccaa aaatcatggt caactctata
    40201 cagggcctgt cttacaatta acagaacaaa aggatgccta cgaatggtca gaaaaaaact
    40261 atcgaattct tccacttttt catccagcag ctattttcta caatcgctcc cttgtacctg
    40321 ttatccatca agactggcaa gtactagctt cacttttaca gcaatattaa aaagaacaga
    40381 caagaccata aaatcttcag tttacagtct tgtctgttct ttttcattgt tttaatggct
    40441 aattgcagta tatttcgcat gtaaagctgt caacgcatga gcaactgttt gatgttgatg
    40501 cgtgaatgta tgttcccaca agtgagaaag ttgtttttct acttctggct gcaaggaagt
    40561 atagataatc gtttgtcctc tttgctctgc tttttcttga aattcatcaa aaactgcacg
    40621 tgccgtcaaa tcaatacttg gtacgcctcg catggagaaa atgacaccat cgtgatgatt
    40681 tagttcttcc aatttatttt tgagtttttc tgaagacata aaaaacagtg gaccactaat
    40741 atacactact gtccagttat tcagcttttc cgtttctggc agccccattc tttgccaatc
    40801 gatcgtttct aatgaaatcg tgatagcagc gcttttgaca acaaaaaata cacagccgcc
    40861 gatcaccccg atcacgatag caatacttaa atcaaagata actgtacaaa tcatcgtaag
    40921 gataaacaat agaactgctg accaatattt attagtaaat agctccttga tcgtttccca
    40981 ttcattcata cgccaagccg tcacaatcaa aacacctgca agtgctggca ttggaatctg
    41041 tgacatcact ggcgcaaaaa caagcatgga gagcagtaag acaactgcat gtatcatacc
    41101 agctaatcta gtttgtgcac cagatttgat ggccacgctt gtacgggcga tggccgcagt
    41161 agcaggaatc ccgccaaaga aaggaagcaa cagatttcca attccttgcg ccattaattc
    41221 ttgattgcta tctaagggct tatttgccat tcggccagcc gaagcaccac atagcaagct
    41281 ttcaatcatt cctaataaag caatgctaat agctggaact gccacttgtt gcaaggcaga
    41341 aaagctaaaa tctcccatat tcaaacgatt actactgatc aatgtttgtg gaatcttacc
    41401 cacagttgct actggtaaat ggaacaatag cataactgct gttgccaaaa tgatagccag
    41461 cagcgaacta ggcattcgtt tcgcccattt ttttggatag ataaacatcc ctaaaatgac
    41521 tagccctccc atgatcaaag aggctgtgtt tatcggaaat cctatcgttt gataactact
    41581 tagtttttca ataacgtttg taccttccga gtgaacacca aataaattat cgatctgacc
    41641 taatgcaata atgatagcaa tacctgaagt aaaccccgta attacgggtg ctgggataaa
    41701 agaagtcagt gttcctaatc ggaaaagtcc tgctgcaagt aaaaatagac ccgctaaaaa
    41761 agtagcaagc aatacacctt gcattccctt tgttgccgca atagacatca agatagctgc
    41821 catcgcacct gtcggacctg atatttgata aaaaccgcct gataaaccgc caataacgat
    41881 accggcaatg atagccgtaa tcaaacctgc ggccgcatca gctccgcttg ataccccaaa
    41941 agccaaggct aaaggcaatg ctacagcagc tactgtgatc ccagccaaaa gatctttctg
    42001 aaatcttgaa agatcataac ctgaaaattc tttttttagt gaggaaacat aatttttgaa
    42061 cacctatcta actcctttt
//
LOCUS       DBKOIJ010000008        38367 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000008 DBKOIJ010000000
VERSION     DBKOIJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.008"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(5..2062)
                     /locus_tag="ACV5NJ_000320"
     CDS             complement(5..2062)
                     /locus_tag="ACV5NJ_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HHY6484450.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2176..2388)
                     /locus_tag="ACV5NJ_000321"
     CDS             complement(2176..2388)
                     /locus_tag="ACV5NJ_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484451.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            2575..3042
                     /locus_tag="ACV5NJ_000322"
     CDS             2575..3042
                     /locus_tag="ACV5NJ_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484452.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            3039..3281
                     /locus_tag="ACV5NJ_000323"
     CDS             3039..3281
                     /locus_tag="ACV5NJ_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HHY6484453.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            3299..3601
                     /locus_tag="ACV5NJ_000324"
     CDS             3299..3601
                     /locus_tag="ACV5NJ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HHY6484454.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            3673..5727
                     /locus_tag="ACV5NJ_000325"
     CDS             3673..5727
                     /locus_tag="ACV5NJ_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484455.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            5727..8339
                     /locus_tag="ACV5NJ_000326"
     CDS             5727..8339
                     /locus_tag="ACV5NJ_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HHY6484456.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            8336..10240
                     /locus_tag="ACV5NJ_000327"
                     /pseudo
     CDS             8336..10240
                     /locus_tag="ACV5NJ_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
     gene            10358..10867
                     /locus_tag="ACV5NJ_000328"
                     /pseudo
     CDS             10358..10867
                     /locus_tag="ACV5NJ_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
     gene            10919..11251
                     /locus_tag="ACV5NJ_000329"
     CDS             10919..11251
                     /locus_tag="ACV5NJ_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484457.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11252..11845
                     /locus_tag="ACV5NJ_000330"
     CDS             11252..11845
                     /locus_tag="ACV5NJ_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484458.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            12008..13702
                     /locus_tag="ACV5NJ_000331"
     CDS             12008..13702
                     /locus_tag="ACV5NJ_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HHY6484459.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            14390..16267
                     /gene="ltrA"
                     /locus_tag="ACV5NJ_000332"
     CDS             14390..16267
                     /gene="ltrA"
                     /locus_tag="ACV5NJ_000332"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HHY6484460.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            16398..16775
                     /locus_tag="ACV5NJ_000333"
                     /pseudo
     CDS             16398..16775
                     /locus_tag="ACV5NJ_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            16775..17980
                     /locus_tag="ACV5NJ_000334"
     CDS             16775..17980
                     /locus_tag="ACV5NJ_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HHY6484461.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            17996..18640
                     /locus_tag="ACV5NJ_000335"
     CDS             17996..18640
                     /locus_tag="ACV5NJ_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484462.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            18651..19457
                     /locus_tag="ACV5NJ_000336"
     CDS             18651..19457
                     /locus_tag="ACV5NJ_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484463.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            19479..19709
                     /locus_tag="ACV5NJ_000337"
     CDS             19479..19709
                     /locus_tag="ACV5NJ_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484464.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            20255..20530
                     /locus_tag="ACV5NJ_000338"
     CDS             20255..20530
                     /locus_tag="ACV5NJ_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484465.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            20572..20994
                     /locus_tag="ACV5NJ_000339"
     CDS             20572..20994
                     /locus_tag="ACV5NJ_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484466.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            21013..22605
                     /gene="mobP2"
                     /locus_tag="ACV5NJ_000340"
     CDS             21013..22605
                     /gene="mobP2"
                     /locus_tag="ACV5NJ_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HHY6484467.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            22617..22952
                     /locus_tag="ACV5NJ_000341"
     CDS             22617..22952
                     /locus_tag="ACV5NJ_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484468.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            23099..23293
                     /locus_tag="ACV5NJ_000342"
     CDS             23099..23293
                     /locus_tag="ACV5NJ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484469.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            23325..25697
                     /locus_tag="ACV5NJ_000343"
     CDS             23325..25697
                     /locus_tag="ACV5NJ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HHY6484470.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            25758..27218
                     /locus_tag="ACV5NJ_000344"
     CDS             25758..27218
                     /locus_tag="ACV5NJ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HHY6484471.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            27229..29433
                     /gene="topB"
                     /locus_tag="ACV5NJ_000345"
     CDS             27229..29433
                     /gene="topB"
                     /locus_tag="ACV5NJ_000345"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HHY6484472.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            29449..29598
                     /locus_tag="ACV5NJ_000346"
     CDS             29449..29598
                     /locus_tag="ACV5NJ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484473.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            29672..30265
                     /locus_tag="ACV5NJ_000347"
     CDS             29672..30265
                     /locus_tag="ACV5NJ_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HHY6484474.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            30281..31180
                     /locus_tag="ACV5NJ_000348"
     CDS             30281..31180
                     /locus_tag="ACV5NJ_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HHY6484475.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            31183..31668
                     /gene="ssb"
                     /locus_tag="ACV5NJ_000349"
     CDS             31183..31668
                     /gene="ssb"
                     /locus_tag="ACV5NJ_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HHY6484476.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            32034..32294
                     /locus_tag="ACV5NJ_000350"
     CDS             32034..32294
                     /locus_tag="ACV5NJ_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484477.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            32735..32941
                     /locus_tag="ACV5NJ_000351"
     CDS             32735..32941
                     /locus_tag="ACV5NJ_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HHY6484478.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            32941..33192
                     /locus_tag="ACV5NJ_000352"
     CDS             32941..33192
                     /locus_tag="ACV5NJ_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484479.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            33207..33644
                     /locus_tag="ACV5NJ_000353"
     CDS             33207..33644
                     /locus_tag="ACV5NJ_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484480.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            33637..34344
                     /locus_tag="ACV5NJ_000354"
     CDS             33637..34344
                     /locus_tag="ACV5NJ_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484481.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            34509..34709
                     /locus_tag="ACV5NJ_000355"
     CDS             34509..34709
                     /locus_tag="ACV5NJ_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484482.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            34725..34904
                     /locus_tag="ACV5NJ_000356"
     CDS             34725..34904
                     /locus_tag="ACV5NJ_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484483.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            35245..35508
                     /locus_tag="ACV5NJ_000357"
     CDS             35245..35508
                     /locus_tag="ACV5NJ_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HHY6484484.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            35502..35852
                     /locus_tag="ACV5NJ_000358"
     CDS             35502..35852
                     /locus_tag="ACV5NJ_000358"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HHY6484485.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(35876..36490)
                     /locus_tag="ACV5NJ_000359"
     CDS             complement(35876..36490)
                     /locus_tag="ACV5NJ_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6484486.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            36804..37226
                     /locus_tag="ACV5NJ_000360"
     CDS             36804..37226
                     /locus_tag="ACV5NJ_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484487.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            37236..37439
                     /locus_tag="ACV5NJ_000361"
     CDS             37236..37439
                     /locus_tag="ACV5NJ_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484488.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            37650..38234
                     /locus_tag="ACV5NJ_000362"
     CDS             37650..38234
                     /locus_tag="ACV5NJ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6484489.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        14048 a         6104 c         7764 g        10451 t
ORIGIN      
        1 tcttttattt gaagattgga ttcgcactaa taaatacatt atctacgcca attctaccag
       61 cagagtcaac tacaaaatta tgacctaatc cgagaatact ttggttatta taaagtaaat
      121 ttgcattatg ggcagggcta tttttccatc ctaccatggc atcgatagca ccatcgtctg
      181 ataaaaggtc atcaatatac agcccagttt gacttatgtt ttctccctta gcaccgtagc
      241 agcctaagct ttccaataaa tcttggaaag taccaccacc agggcgatag tgactgaagc
      301 tgacttgtaa ttctttagct cgaatatctg cagcctgttg aacaatatca ttgaattgca
      361 ttggggcaac accattttgt tggcgataat catttaacca agcaagttct ttttgacgca
      421 taaagttacg taattcgtct aatgaaatct ttttatattt gaatgtgatt gttttatttt
      481 gatcgacgtt attaatggat cgttggccgt ttttatccac ggtaacggta ggatcgtcat
      541 ttctgcacaa gaaataattt tgatcagtca tgtttttcca aggagtagca aagctatccc
      601 cttcgtatac tgtttttgat tcactagaga gaacagcgtt ggtatcagca tttacatgat
      661 taacagtcac attaaattta cgagtgtagt tgaaaatata attttgactt tcagtcacat
      721 tgtttaaggt tactgaattt gttggttcta cccgatcgtc taaaacatat ccttgaatag
      781 aaggagcagt taaagtgaca ctgtcaccgt gagtgacatt gactatctgt tctttcaaaa
      841 tattaactcg tccttcgttt ttgtagttgt tattgcacaa caaattgatt tgatgcactt
      901 gattataatt gaatgtgatt gttgtattac ctgtgattgt tttgctttga gtatcattac
      961 caatcacttt gtagctaaaa ttatctttag cgccttcgct atatttaaat gcttttgcct
     1021 tagcagtgta agtagaacca gaagttactt ttgcttgatc tgtagcaatc acttgacctg
     1081 tttgattgtc aatatggtta atagtaaccg tatattcttt tggtgtttca ggtttgtgtt
     1141 gcaacccttt aaatgcagca tcaagactat ttttagcatt gtttacttgt gtttgagaaa
     1201 ccttagaatc gtctagtact tttttagcat tggataaagc agtctgaaaa ttattccaag
     1261 attcttctgt aaaatctcct ttagcagttg attttactcg gttgtataaa gcttgtaatt
     1321 cagctttatt cacagtagga actggttttt tctttaatcc tttgtaagca gaatctaatt
     1381 tatttttggc actatccact tgcttttgag tcgcttttga gtcatttaac actttcttag
     1441 cattgtttaa agcggtttgg aatgcgttcc aggtattgtc ggtatagtca ccttttttcg
     1501 ttcccttaac gctcgtataa agagcttcta gagccgattt attcactttg ggtttagagg
     1561 tgtcagcgcc ataccaaccg ataccttcgt atttccagcc atagtctttg gttaaccatt
     1621 gtacttcttt ccaacttgca gtgtagtgat gtgagccaga ttttacacca ggaaggaaaa
     1681 ggcggtagat cggtttatta tttttttcgg cagaacgcca ggcaggccct tcataagtcc
     1741 agccgtaatt tttagtcaac catttcactt catcccaact agctgtgtaa tggtgatcac
     1801 gaagaccttt attgtacaaa cgatagacaa gatggccacc gctagtcggt gcttcccagg
     1861 cggggccttc ataatttccc cattttatct ttactagatg gtcacgttct tttttgctag
     1921 aggtgtaaaa atgttcatta ttatttggat tgtaaaggcg atacatgacg ttagttgaag
     1981 cagcgtcaac agatccagca agactaagtg aggcagcaaa tgttccaata gctaaaacag
     2041 tttttggatt aaataatttc attttttcaa ctcctaaatt taattaaaaa tgtttacatg
     2101 attattatat atcggctaac cgatatagtc aataaaaaat aagaaaagcc ttatataatt
     2161 ttggcttttc ttattttatt tgttagttga agtattaata tcaacgattt cttcagccaa
     2221 tttatttaat ttcatagcat ttatgaatct catgttgtct atactagagc gtttgttggc
     2281 aaggtcactt actgtagtta agggtacgcc agattctatg gatatagaac gaactgattt
     2341 accactatca aacagcaacc attcgatttt ctttttatca gcgtacattt caatgctcct
     2401 tcttgttttt tctttattat agctcttttt tgtaatgaat cacaatatca agagaaaaat
     2461 tttctataga aaggtggatt tttatgagta ttcgttctcc gtgtatccaa atgaggtttg
     2521 ttgctacttt cgttatttga tatactaaaa ataaacaacg aaagaaggaa taaaatgaag
     2581 aaaaagcaac tatggtttgt ctctatgttt gcaatatttt ttattatttt aagtggatgt
     2641 tcaagtaata agaaatcgga agctaaagaa gataatagtt tattgcaacc aggaactgtt
     2701 tggaaagaag aagtaggtgg tcttattcat aatttaaaaa ttatagatga tactacctgg
     2761 gaatattctg aaagtgtatg gcatccagat cctgtacaaa taacagttaa gaggcaaaaa
     2821 gactatgaag gtttagaaag atacaaaata gtagattctg caggcattag ggaatttata
     2881 aacaaacctg attcattatt tattgttgtt ccctatgaga aggatggcgt aaaaaaaata
     2941 atttttcttg gatctagcaa ggatgagaag cgaacgaaag agaaattaat tcatgatgga
     3001 tcagagttaa ataaatacaa attacaaaag actagtgagt gattgctagt ctttttattt
     3061 atcctgaagg aggaatcaca gtggcaaaag ttaaaacacc attcgaaaaa gccgcgtcta
     3121 aaatcttaga acgccgtggt gtttctgaaa tacagtggaa aaaagaagtt cttgaaaaag
     3181 cacaatttga cctattacga ggaaaagata aggacttaga agaatatatc tatcaacgtg
     3241 aacgtgaaaa acttgtattc gatgaaatta ttaaacatta aaaggagcga ataatcttat
     3301 gtttgaatca attacaatga attttttaac tcaaccgaaa gtagtagctg cagttgatat
     3361 ttttagagca ttacaaactg ccgcaactag tttgaaaaat aatctgatga tttttgcggc
     3421 tgtagctgcc gttgcctcca ttgctcttgg aggtattttc ttcttctttg ggcgaagagg
     3481 agctgaaaac gggaagacac acgtaggtta tgtattgttg ggggtaggag ttattttagc
     3541 tgccgcttct attgtagttt caatgttctc aatgtttgga caaaccccac aaggactata
     3601 aaaaaaacaa gtaggaggag taaaaagtac gtggctactt tttactcctt tttttatgaa
     3661 aggagaatat tcatgttttt tacgatttca ggaaatgcaa aattggataa agagttaata
     3721 agaaaagcaa cgaagaaagt gggaaagcgt acttcctttt taccgaaaac aattaaatat
     3781 tctgtcattg aaacggaaga acagctacat gtgttaggac aagtgttaga acaagagaat
     3841 caatcttctg ttaaattgac tctcacgtta catgataaag aaggaaaaat agaaaaggct
     3901 ggcacagaat acgtttttgg cccactggat tatcaaattt cagaaggttt ttatcctaca
     3961 ttgatagatt taatccgcct tgatgtgttt aaagatacgg atgaaactat gagtgtggaa
     4021 gagtcttatc gaaacgaaaa actgctacaa gttttagaag aagagttaga gagcgaccaa
     4081 agaaaagtga atcaagtgag tgaagtcgaa gtaaatgaag aagtaccatg ggaacgacca
     4141 atctttccaa agaaggagtc aaaagaaaaa gagacagtct atgttgacga acaagctgat
     4201 attccgactc agaaatcgat atttgaggaa gagaacgatg tgatgggtca attggaggta
     4261 gatccctttg aagaaacaat ggaggatgaa gagacggacg aagtagaagt tgatccagtt
     4321 gaagctaatg aagagtcaac atggactgaa ggggattttc aggaatatat tgaaccactt
     4381 atcagtgaag aagtagaaaa aaacgaaaca gagatacaaa aggcaactgt atttgattat
     4441 attttggatc aatatccgcc agagcatgaa tggatgaaag aaaaaaacaa attattttta
     4501 aatgagcttt attcgaaata tcaattaccc caaacctatc aagcattttt agagagtaaa
     4561 gaagaattaa tctcacaggg taaaggcaca cttttagaac agttagagtc tgtccaatca
     4621 acaaattggc atgagcttgc acaagaagat ttattagcca cctttgaaga acgtcaacaa
     4681 gaaacagatc aagaaattaa tgagtatgcg acggaacagt cgtctaattg ggagaaagta
     4741 aaagaggaac taaataaaga agaacagcga gtgattgaag aagaagtaca aaaagtcaaa
     4801 gagcggtacg aaaagaaacg cgacaatgct tattccttgt gtgtagaaaa gatcaacaat
     4861 tttactaaga cgaatcaaga agcgttatcg aaagaaaaag aaaacttgtt aaaagaacgt
     4921 attaaagaac gaaaagaaga gttctaccgc ggtttacgta cagataagct aaagattagc
     4981 aaagaattaa atgaacaact gaataagcta tatcaaacaa cgactgaagt tcttatggaa
     5041 aaacacgaac agatccagca agaattacaa gtacaaattc caaaatggaa aaaagagtat
     5101 gaaattcaac aacaagaatt gaaagaacaa gaagaaaaac aacggcaaaa agaaaaagag
     5161 cgtgaacaat tagaaataag aaaacgggaa caggatcttg cggaacaaaa aatggcgata
     5221 gaaaaacaac ggttagagca agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa
     5281 gaacaaatgg atacattgcg tcaagcacag ttgaattatc tagcacaacc tcaaccagta
     5341 gctgctgttc aaacgccagt gaacaaccaa gcaaatttaa ctccttcacc ccagcgttcc
     5401 tttaacggat ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca
     5461 gtatttgctt ttaatcatat caatcaatct tcacaggctg attctgtagc cacaacggcg
     5521 tcaatcaagg aagaagccca ggaacaagca agaaaagagt atgaacaaca attagaagaa
     5581 ttattgaatc gtaccaatca gtcaacaaca gagtcaagca tggaaaatag caaagagagt
     5641 gatcctagta aggctaaaga aggatcaagc gattcaactg tacactcaaa cacttccgaa
     5701 agttcagaat caaatgaggt gaaataatgc gtattagaga aacaacgaga cgaaaagaca
     5761 aacgattcaa tttagaaaag aataggtatc tttctcattt ctatcgctat actccttcag
     5821 gagaagagct accagaaaaa catgacaaac gtatttctca aaagattttt tatagtggct
     5881 tagcttatct tcctttaaac tatgttgtta caagaatacc agaagcattt catcgatttg
     5941 agattattcc aggaagttta caaggagtga atatttttca gttacaagaa atgccgcagt
     6001 tattccattt tgattcaact ggtttttgga atcctttgac cccaacaaca agtttaggca
     6061 cacgtttagt tgtttctacg atttgtgttg ttccagctgt acttatggga ttgaaaatgg
     6121 acatgtggtt tcgtccgcta gcagatggac aaaaagggga taatcgatta atgaccttaa
     6181 aagaaatcca acagatttat ccgcaaatac ctgaaacaaa aactactttt ccaggttatg
     6241 gaggtattcc aatcacacat tacaaaaaat attggtacat tcaaacagat actgttaata
     6301 cgtgtattgt tggtacctct cgatcaggga aaggtcagat tgaagtgtta gccaccattg
     6361 acaatttatc tcgtgcagaa aaacaaagtt caatggtcgt taatgatcca aaaacagaat
     6421 tatatgttgg tagtaaagat gaactagaag ctagagggta tgatgtatat gcctttaata
     6481 tcaccgatcc actgcaatca atgagtgata atccattggc actcatcgtg aaatactgga
     6541 aacgaggaga catagatact gcaacacagt taaccaatac tttcacaaat acgatttatt
     6601 atgatgccaa cgcttcagat aacaaatact ttaacgaaaa cgcgcagaaa gctgtcaatg
     6661 cgcttatttt tgcgttgtta gaacaagctg atcaaacagg agattactcc aaattgacac
     6721 cgaataatct cgttgagctt ctttctgaga ttggcggttt taattattat ccagatccag
     6781 acaatgaatt caaacagttg aatgccttag atgaatttat gaatcagttg ccacctggaa
     6841 acattgcaaa aaaacaatat ggggcaacga aaattggatc tgataaagcg aagggaaata
     6901 ttttatcaac ggcaatcacg ggattgaatc catttactct aacgaaaatt gcaaagatga
     6961 cttctcaatc aacgattgat tataaatcgg ttgggtttcc aaaatactta gatttgaaat
     7021 tacatgaaag tcttttaaat caacgagtcg tagtagaatt ttatcataag ggaaagaaat
     7081 ttcatgaaga acgtgtcaaa gtaggctata aaggatttag tgaaatcaat tttgattgtc
     7141 atttagttgc aggtgatcaa gtttgtttaa aatacgatca tcaaggaaaa gaatatgtgg
     7201 cttggtatga attttctcaa agagtattga aagacgaaga gggtaatatc gtttatcgga
     7261 aaaaacgagg agaaacccat ttgccagaat tagaaaaaga agccgttcta acattagatg
     7321 aggaaaaaag taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg
     7381 ctatttttat gcttacacct gattatgatc catctaacca tatcattgtt agtatttttc
     7441 ttagtcagtt atataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc
     7501 accgcaggat tcattttatt ttagatgaat ttgggaatat gccaccaatg gataatatgg
     7561 aaggaatcat gacggtcaca gctggccgaa acatgttgtg ggatctgttt attcaatctt
     7621 atcagcaact ttatgcgaag tatggagaac aaaacgcagc gattatcaaa gaaaattgtc
     7681 agacacatat ttatattatg tcgacagatg ataataccat cgaagaattt tctaaaaaag
     7741 tggggaataa gacggttgaa caagagaaca gtaccatcaa ttcattaggt atgaatactc
     7801 atatcaatcg caatgttgat tcagatcgta tcttaactcc tgaaagaatt tctacttttt
     7861 tagaaggaga aacgattgta ctcgctcctt taaaacgaca agatttgaaa ggtcataaaa
     7921 tacggccgta tcctattttt aatcatgggg aaacgaatct tccttatgct tatcaatata
     7981 ttgaagattt caatccatcg tgggatatca atgatataga catacattgt acacatgcgg
     8041 acttggatct taaggcttta caaattgatt atgattattt cctaccagat gaatatgcta
     8101 gaaatctcta tcatggactg catgatcaat tagaagaaaa aaacgaagaa aatatgtttt
     8161 ataaaatcgt tcaatctttt gagaatgaga cggctaaaaa attgttaatt caattttacg
     8221 aagaacaagc agcagatcga cttcaagaat atgttgaaaa gttgatcaaa aataaagaaa
     8281 tttctgaaat gattgggaaa cggttgttat tggaatctga aaaggtggtg aaagaatgat
     8341 cattggagga attttttctg gcatagccga ctggtggtca ggcttattcg gagaagggga
     8401 tgcaaacccc aaaaccgtaa cgaaagtctt acaagattat gggaattatc tgcagtatca
     8461 agatgtattt agttggattt ggaacacttt tctgtgggga ttgattaaag gattatataa
     8521 tctcaatact ttactagaaa aaaccattta tcaaagcttt gatttaaaag acttattgaa
     8581 cgccgctggg gtaaatgcac tatttcaaag tttgattagt aaggtattgg ctcttttttg
     8641 tgtcgtcaca ttaatttatt taggcttgaa attttttatg agtaaacacc caccaaaagt
     8701 caaaaatatc ttggttaatt tatttatggc catgatcttg atttttggtg gtagttcttt
     8761 aatcgatgaa gggttaaata tcagtaaaag cttttacgga gacgtcactg cagcaaataa
     8821 agcagataaa acgggatcac ctgcttttca aattattcaa aataatgtga tcgatgtcag
     8881 tacgttacta gcaactgacc cgaataaagt agaatcccta ccaacagata aaagaaatgc
     8941 tttaacagcg aaaaatttta aaacagcgaa tatcaatggg attattactc ctgaacaagc
     9001 taaaggtatg gcaggtaatg ataaaagtgg gttatcagat atacaaaagg atcggttaaa
     9061 ggcattacaa taccgattgg aactagatga taatgggaaa gaaattcctg ttcaaattag
     9121 tgacgaaggc ttagcaaaat atgtctatac ttcagggtat cgtcgataca gtagtaaacc
     9181 aggtattatt ttagctggtg aatcttcttt ggcagtagcc tatttattta tcctatttac
     9241 gatcgttact tgcattattg agctggtatt taaaaaattc tatttagtga ttgccgcgtc
     9301 aacggacttt gagacaggtc aacggatgaa aagtgcgata gaagatattt ctcaatcctt
     9361 cttgttattg gcgtttacga ttttagaatt acgaatctat accttaatgc tttcaggtat
     9421 cggtgatttg catgcggcag gaaagatcaa tggattcttg tatgtcgttg ctttgattgt
     9481 actaaccatt gcgctattca aaggctctca atcagttacg aaaattttcg gcgttgatac
     9541 aagcctgaaa aacggcagta attctcttct tagcatgttt gccttaggaa atattgcgaa
     9601 aaatgtgggt agcggagcta aaaaccttgc aagtgctggt aagagtggtt taagccattt
     9661 aaatgatatt cgtaaaaatg gatttggcca tcgaaaagat gttacccctg ataacgggga
     9721 agaacaatca gacgataaag ctacaactgt acctaatagt aagaaaacag tttccgaaat
     9781 gttcgggaaa gcgaaaaatg gtgtttctaa agccgcagaa ggtcttggtt acgtcaatga
     9841 acgtgggtta aaaggaacag ctgaagatct tacagaaaaa gccaaagaca ccttagaaga
     9901 taaattagac ggcacgagag ctaaagaatt ggctgaagct gtgaagaatc catccgaagc
     9961 actagaaaaa caaaaagaaa aagcttcaga agtcaaagaa ggactaaaag atgcgtatgg
    10021 tgaaggacaa gtcaatgcag cagtgaaagc taatacaaca accgaaaaag aaaatcaaga
    10081 aggtatagtg attccttcag tcaaagatca ccaagaagcg ttgcactcta atgaaaattc
    10141 gattgaagag aaaaattcca tagaagaaag aaattcagaa gaaggaaaaa atggacagtt
    10201 accaaaaact gatccagaac aatatcgcta taaacctaac agtgaccttg aaaatgagaa
    10261 gcgaatttca gatttatcaa aagtaaatca agaagacaaa gcagaaaatg agggacagtt
    10321 accaaaaact gatccagaac aatatcggta tagatctgtt aactccgaaa atgaaaaacg
    10381 catttcagat ttaccaaaac gaaatcaaga agtacaaacg gatgaggatt ccatcgttct
    10441 gccaaatgat aatggaatcg aagttggaac tgaaaaatca acgaaaattg gtttacctaa
    10501 ggcacaaaat ttaccaatga aagatagtcg tttgaatcaa ttcaaggata atgatcaaag
    10561 ttttgttcat caatcacctg caggtactgt tcaaatgaaa ccaagtcgtt tgcctgaaaa
    10621 tggaaatctg acaactgttt cttacaattt atctgaaagt ggtgagacag ggaatgcgtc
    10681 cgttcaaaat gttgtgacaa gagaagctgc accaaaacct attcaaaacg ttcatacagg
    10741 ttacaagcca gcaaatgtgg catttactgc accaacaaaa ataaatgtga caagagaaac
    10801 aaaaaacagg aacaatgcat tgaaagaaca actattgagg gatatcaaac aggatatcaa
    10861 accataaaag gcctacaagt taggcctttt ttctatgtat gaaaaggaga aatcatttat
    10921 ggataaatat agtaacatga aaaaccctcc aagatttagt ggagaatgga agtttttcgc
    10981 ttttttcttt ttagatctat taacgcttgg tggctgcttt ttttgggcga aaatggtcaa
    11041 taatcttgtc cacttaccac tcttttttgc actcttgaat tatggcttat ggattgcgat
    11101 agcgttagtg attgtttggc gaccctataa taacccaggt caacgacaaa tttttgcgat
    11161 tatcgagtct attttttttg cggacaagaa ccattatcat gcgttagatc ctaaccgaaa
    11221 ttatcatggt acgtataagg aggatgaata aatgcgagtc aaaggacaaa attcagttga
    11281 aaggaaatcc attttgcagt ttggaaaacc agaagagcgc tctatcttgg acttatttcc
    11341 atataaatcc tgtacagaag agggagtctt aattactaga gaagatcatt ttcaacgtta
    11401 ttatcgtgta gtttctacag acgtggaagg attaaatgaa caagagaaac tagaacgaat
    11461 gaatcagttg accaccgtca tgcgtacgta tgtgccaaca attaagttag ttagtttgac
    11521 cacagagacg gatctaacgg aacaaatcgt gcaaaagcgg caattattag ataaaaatcg
    11581 tatggaacag tccttaggaa gaaatctaca acgattaaaa aaatatgaaa agaaattagt
    11641 cgaagaaatc caggaattga aacgagcaga acaagaacgt cctgacttaa gcttcttttt
    11701 cgtcattgaa ggaaagacaa tcaaggattt acaaaccaga aaccgtcagt taatgcgatc
    11761 tagtggcatt ttagggttaa aaccactcta caaaaaagaa ttgatcaaga ttctgtatcg
    11821 aatgaacaat atgaatgatg agtaggaatg gagaagagag aatgaaatat ccgttgatat
    11881 ggttagaaaa agatgttccg aaggaagaga ccactagaaa atttcctcac actttaagcc
    11941 tgggtaagtt gggaaaaggg caatctttta accaagtaaa gaatcaaaca agaagaggtg
    12001 actgtacatg ttagaagcaa ttaaaacagc tttttcacca caaaatgatc aaaaagtact
    12061 tgatccatta aagcaactag gaaatcggga aatagaaatt gtaaaatcca aagggtatga
    12121 tttagatttt ttaaaacaag tgatgccaca aggtgggtta aattttgatg aagaatatgt
    12181 gacgtatggt aatggttatt cacgatgtgt gacagtttat cactacacaa aaaatcctac
    12241 gcttttttgg ttggccaatc tcatgaacaa tcctcataca gtggccacac tcgatgttag
    12301 cacagatgaa aagcaaaaag tgattgatca aatcaatcgt gccttagatg aattaaatga
    12361 tcgatcagat aatgaacgaa aagcgactga ccgaaataaa agcgcggaag aatacctgga
    12421 actagcggaa tatgctagaa aattgacttc taatggggaa atttctaagc aagtcaaagt
    12481 gagagtacat gtttacggaa acacgattca agaagtagat gaacgagcgc aggaactgat
    12541 cgaaaatctc aaagggatgg attatcgtgc ggtgacttat ctgcataaaa ccaaacgaga
    12601 ttggcaatcg atgtttacca tgttcgatga acaagctgaa tggttcggaa atatcaaagg
    12661 gcaatcacta ccatcgttaa acattggcgg aggtttcccg ttccaccacc aaacgctaaa
    12721 agatccagga gggacacaat atggggaaac catgacaggc ggcccatttg tctggaatgc
    12781 gacgtataaa aatagtgtac ggttgtctac taataccttg cttctaggaa tgatgggagc
    12841 tggtaaatcg accgttctta aaatgatcgc ggaagcccat ttagctgcag gagactttat
    12901 ttggggcttt gaaaagggaa aagactttat tcctttctta aaggaatata acggtattat
    12961 ggtgcgttta gacggatctg acggaatgat caatccttta gagatatttg ccacacggac
    13021 gtatgatgaa gcatccagtt tatatgacga tggatcagtc aaagatttga agatcaatga
    13081 agcagcgtcc tatcaaacac acttagacaa agtggtctat caggtacagt tagtgagtcc
    13141 tcagctaaaa ggaacgatga aagcagaatt taagacctac ttaaatcgtt tttatgaaga
    13201 gtatggaaca gtaccacgcg gctttacttc ctcaaattcg cgttcaaata ctgatacgca
    13261 agtcacaggg aaagatccag aagcttatcc gacgttcaaa gaatttttag actttttagg
    13321 acaactggaa ttaccaggtg cttcccaaga gaaaaagaat cgaaaagaag aaatggaatc
    13381 catcgtagaa tccttatgtg agacgtatgg catgattttt gatggtcata gtacgattcg
    13441 acaccttgac caacaacagt tggtcttttt tgacattgac tctattacag gcttagatcg
    13501 aggggtacaa caatgtcaaa tgtacatggc tatgacgttg atttggaatc aggcattgat
    13561 tcaaggaaga agacaacgac gtttaattaa agaaaaacaa atggaagaac aagacttcaa
    13621 acgcttcttg gcgatgattg atgagtgcca taatggtgcg acctgttctg cagtgaaaag
    13681 ctgtgggttg caagtagtct aaaaactatt gtaataactc ttataacgag aagtcaaatg
    13741 agtgggtaac gccacaaagg gcttccactg atactccgac atgcaacaag tagtttatgc
    13801 agaccaacgt tgtgtgaagc tcggtgaagt cggctgaagg ataccgtaag gtaaaagaaa
    13861 tagtgaatac cacgaaagtt ctctagaggt aaagggtgta tccgataagt cgggtgtcca
    13921 aaaaaactta atatggtgag tatgtacaaa cgtactgacg aactagcgaa tgtactgtcc
    13981 gaaaggcata gctcctagaa atgggagaga cagtgagcaa aaaatcactc attgtgctaa
    14041 cttgtggttg agtaaaagtc gttgttatga aagaccatgc tgcgttacag gcgtaggaga
    14101 agttaggggg caaaagctag catctaagtt aaggcatgtg gatagatata agggaacagg
    14161 ggaagccatc aacatggaga gtgcatactc gatgaagtgg tagtaagaaa tatgtgaatg
    14221 acaaataact tactttagtg atggtgaaag tagagtcacg actgatgaag gttctgtaat
    14281 ggaactggag gaacaggctc gattcgtctg taaaagtcat gaaacccgtt caaccatggg
    14341 attctcgtat gactaaaaag gtcacgagcc aattggagcg tgataaacca tgacaaaaga
    14401 aacaagtcca aaagatagga agttgcggca tgcagaatat tatggcatga cggaaatctt
    14461 cgataagata tactccaaaa gtttgaaggg agataatttc aagaatctaa tgcagattat
    14521 catttctgat aacaacattg tcctagcata tcgtaatatc aagagaaatc acggtagtgt
    14581 gacaagagga gaagatggag taacgattaa agatattgaa aatctcaacc gtaatgaatt
    14641 cgtagaaatt gttaggaagc gattcgcaca ttataaccct cggaaagtaa ggcgtgtaga
    14701 aattcctaaa tataatggta aattaagacc gttgggtatt ccatcaatat gggatagaat
    14761 cgcgcagcaa tgcatcttac aagtattaga accaatatgt gaagcaaaat tcaataatca
    14821 cagctatggt ttccgcccaa atagatcagc agaacacgca atagcagatt gtatggcccg
    14881 aatgaatcaa agtcacatgg aatatgtggt agatgtagat atcaaaggtt tcttcgacga
    14941 agtcaatcat actaaactca tgagacaaat ctggtctatg ggtatacgag acaaacagct
    15001 tctcttaatc attaggaaaa tgttaagagc acccgtagta cttccaaatg ggaagttgat
    15061 atacccaaca aaaggaacac cacaaggcgg aatacttagc ccattacttg cgaacatcaa
    15121 tctcaacgaa tttgactggt ggatagcaaa tcagtgggag gaaaaagatg ccaaagagct
    15181 aaaacatcat tttaggaaaa atggagcttg ggataaaggt aatatctatc acaagctcag
    15241 aaagagtaca actctaaaag aaatatacct agtgagatac gcagatgatt ttaaaatttt
    15301 ctgtagaaat tataatgaag cgaaaaaagt tttctatgca actaaaatgt ggttacagga
    15361 gcgtctaaag ttaccaatat ctgaagaaaa gtcaaaaatc acaaatcttt caaaagaaga
    15421 aagtgaattt ttaggcttta cgatgaaatt agtgaccaaa ggtaacaaaa aggttgttaa
    15481 tacgcatata ggtacaaaag cacaaagcat gattaaagtg aaactaaaaa aacaaatcaa
    15541 acgaattcaa aaaagtccta atagcaaaga tacctttatc tatattggag tctataacag
    15601 tatggttatt gggattcata attacttcaa aattgcgact cattgtagtg tagcaatgcg
    15661 aaaaattgca agagaactca gaccaagttt tgaaatacga ctacgagaac aaggaatctc
    15721 aaaagaagga caatacggaa agtacgaagg atacaagcct tacctacggt caggacaaat
    15781 acgatatata agagggtttc caatattacc agtagggtat attcaacaca gacatccgat
    15841 gtgtaaaaaa agaaatatta ataaatacac cgaagaaggt agggaagcca ttcataagaa
    15901 gcaacaaacc gtagaagaat ggaagatatc gtggttgtgt agcaatccag tgcttagtaa
    15961 gagagcaacg attgaatttg cggacaatcg aatttcaaaa ttcatcgctc aaaagggaag
    16021 atgtgcagta acaggtgagg agcttatctt atctgaaatg cattgtcatc atattatccc
    16081 ttatcatgaa agcaaaagtg atagctatga aaacctagtg attgttactg aggaggttca
    16141 tcgggtaata cacgctacac aaagtgaaac gattgaggaa cttctaaaat atctaaaatt
    16201 aaatccaaaa caaaaagaaa aactcaatga gttgagatta aaagttggta acgaagaaat
    16261 atcttaataa atatttgtag gacgggaaaa cagaatgtct tttatagacg agggaacgcc
    16321 gtatgcggtg aaagtcgcac gtacggtgtg gactggggga aaagacggag ataatatcaa
    16381 actcttacct atcagtatcg attaatccag attttattga aacggtaaat tatgtcacaa
    16441 actttcaacg agaaatgcgt aaagtagaag caatgactgt tttagctaca caatccccac
    16501 aagaaatggt gccagaaaat atgtcttctg ataacttctc gaaactgaag aaagtctttg
    16561 aattttcttc tacgaagatc tttatgcgta tggatgaatc tattttacgt catatagaag
    16621 gattagtggg taaatcaatg acgaactcag aattagaatc cattcctcaa caaaatcaag
    16681 gagacgctgt gatcaatttt ggctcaaaag aaaccattcg tgttcatttt agtccgaaca
    16741 aacgtcaatt aaaattgttt gatggaggaa aataatggaa aaccaaaatg aaagtttaat
    16801 caagcagtat gttaagcgtc gtgcaaagcg gcgcttattt ttgtggttgt ttggtacttc
    16861 tgcaggtttg attactattt tgatcactgt gttcgtgacc ctatttctaa tacttgcggc
    16921 gggctcgatt gataattcgg attcagattc aagcagtggt ggcgaagctt ttacaggaga
    16981 atattcggaa ggcttaccga tttataaaga aatcaaagga agaggccctt tttctgatga
    17041 gattgcgcaa tatgccgtag gtgcagcagt gaaatataaa ttattaccaa gtgtgatcct
    17101 ttcgcaatac ggttacgaat cagcgtttgg tacatcggcg tccgcaagaa atgatctcaa
    17161 ctattttggg attacttggt ttgatggttg tctgtttcct aaaggaactg cacgaggaat
    17221 tggtggcatt gaaggtggct ggtacatgaa gtttcccaac tcaaaagctg cattttccta
    17281 ttacggcttt atggtagcca cacaatccaa ttttaatgct tgtgtaggaa ataaaagccc
    17341 tggcgcaagc ttattgatct taggacgagg cggttatgcc gctgcaggaa ttacggaaga
    17401 tagtccgtat tatacaggtt gtatgtcaat tatcacttct aataaattaa ccgaatatga
    17461 tgaattcgcc atcaaacatt ggggtgaagg tggcaataac aacggcacga ttactggtga
    17521 atggacgaat ccattccctg gcagcagttt agataaaagt tccttcagcg gtggtcaatt
    17581 atttggaacg aatccaggcg gtgaattccg tccgaacggc ttccatgatg gattagattt
    17641 tggttcagtt gatcatccag gaagtgagat tcatgcggtt catggcggaa aagtcgtgta
    17701 cgttggcaat cctggtattt caggtttggg tgcgtgtgtg atcgtgatta attacgatgg
    17761 cttgaatatg gtgtatcaag aatttgcaaa tagtacagga aattctcgag tgaaagtagg
    17821 cgatcaagtg aaagttggtc aagtaattgg gatacgagat acagcacact tgcatttagg
    17881 atttactcga atggattggc gtcaagcaca aggacatgca ttcattgatg atggtacatg
    17941 gatcgatccg ttaccatttt tgaatagcag taaaaaatag atcaggagaa aaaatatgga
    18001 aaataaaacg ttaaaaatcg tcttaacagc tgcaattagc gttagtttaa tcggcaatat
    18061 tgcgttgttt tggcagctga aagaggaaca agcagcaaat acctataaaa ttgaagaagt
    18121 ggctaaaaac gcgtctgaga agctcaaaaa aaattcctct gaggcacagg cgaagatcga
    18181 caagttaaat caaaccatca atgagaaaaa ccaagaaatc gatcagctga agcaacaaga
    18241 tggcacaaaa gcaaaagagg aagtaactga agcacaaaga aagacggcag aagagttcgg
    18301 tcaattggcc attgataaat cattaagcag aaatgaatta acggaaaaac taaaagatgt
    18361 cgcaacccaa gaagtgatcg acaaattaag tcctacagac gatgatcacc aacatgatga
    18421 ttacggaagt tactccttta cattaggtga cgtgcaaact tacgctcaaa caagcagtgt
    18481 aaaagaggaa caaaattttg tcgtatttgt tgattataca atcggcaatc cgcaattcaa
    18541 agatgtcaaa ccacaaaaaa taaaaggtgg attaactgtc actgaaaaac aagtagatgg
    18601 tcaatggaaa gtcatagact ttagttattt cactcgttag gaggaagaaa atgagtaaga
    18661 aaaaaggaga aagacaacgt ttcaaaaaac gaaaaagtta tcttagatgg ctgggaatct
    18721 ttatgattct cggctttttg attcttgcta gtccgattta tacgaatcag aaggtagata
    18781 taccagatga ggtaagcacg aacgcctttt taaacacgaa agagctgaca atggtttcaa
    18841 aagaaaaagg agcgaaccag caattagttt ttgaatttgc gatcgatgat gaagatcaaa
    18901 gcttgttaaa agaactggct aatcttaatt atcgcgtaga agtaaagaca gctaaagggg
    18961 attatcaagc aatacgagcc aaagtgatta aggtgtctga tgattatttt gcagtgaagc
    19021 taacgcatgt accagaagaa tatatcgcca tgcgtttaac cattattcct gaaaaaattg
    19081 atccaaaagt ggatatgcaa gaaccacaag atctgatttt ttatattcac gaagataagg
    19141 tcaaagatca cgtgaaagac gaagattatg aacaacatgc gatcaattac aaagtgaaag
    19201 gatacaagaa agaacaaaaa gcagctcaaa aacaaatgga atctttccaa gcaaccattg
    19261 atttgaatga agaattagtg aagaagctaa aagatcaatt gccttatcaa gtcgcagatg
    19321 atcaggaaaa tactgaaaac aagattaata gctaccagca agaaattgaa acatcgaaaa
    19381 cgcaactgaa agatcaagaa gagatcattc aaaaacttca agaaaaaatt gatcgtatga
    19441 caaaagaaaa gatttagaag gacaggtgga gtgaaaaaat gaagcgaaca aacttggctg
    19501 acaaagatac tttcgctgag cagaaagtat tgattaaagc ctatctcaaa gttgtaaaag
    19561 ctaaagaaat atgtcgagaa gtgaatttat ctaacacaac aatatctgag ttgaagaatg
    19621 gtcgaaagaa tttagatacc ttaaaattca gatcatttga agctctatat gtagctgcta
    19681 aaaaatattt tacagctgat aacaaataaa aaacaggagt attttatgtg aaaaaagtat
    19741 ataaaaaatg cttatgaatc agctatatca ccttaaccat ttgttaatga gatgggagaa
    19801 aacattaacc acgattctaa cgcaaacatc aagcacataa aacatttaac actcctcctt
    19861 tctggctgaa gatttcaacc aaacaggaag acagcgtatc aattatagca tacacttttt
    19921 cttaaattca tttttcatat gacaaaatat ttccttctaa tcgacaaagt tagaaggatt
    19981 ttttattgtc taaatacaca aataacctta atttttaaag agcataagga aacgtgatat
    20041 cactttctca cgaaaggtgt taggtaggct gtgcctacca cgtttctaag aaagataaat
    20101 gatatcaaaa ttttggattt gtgtgtagat ttgtgggtag gatgaaagcg aatgtgggca
    20161 gtaaaataag cgtttgtgtg tagcaaaatc aaaaaatgtg tagggatatg tgtagctaca
    20221 catatcccta cacataataa atttggagga aaatatggac ttattaatta gaaatattga
    20281 agaaaagtat attaataaaa ttgacaaacg ttgtgaagaa cttacggtca agactggcaa
    20341 aaaatggagt cgaaatcaat acctaaaagt tttgatcgaa aatgattttg atcacgctct
    20401 tctgaattat aaaaaagacc aatttgatcg gttgttagaa aaatttgtgg atattcaaac
    20461 ttataatacg aaaactttag aagaatatat tgcaacaaat aatcaattaa ttggtttgtt
    20521 aattgaataa taaaaatagt attgtgaatt tataaaagga ggaaatcaat tttggcaaaa
    20581 gcaacaagaa aacatgtata tctttacgat atgcatatcg aagtacttga tgaaataatt
    20641 tctactcatg aagatatatc taattatagt gaagcagtca gacatttgtg tatgatgtat
    20701 gatccaaaaa tcgaagaaaa aataaatcca attcaaatta aattgaatgc aatgggaaaa
    20761 gaaatttcta ttttaaccga aatggtttca gagtttgcgg atcttcattt gaaagataca
    20821 ggaatactaa ttggtacaaa ttcaactgtt tatcaagaag cagaagaacg tgtggaagga
    20881 aaaattaaat ccaatcaaac caaaaaatat tcttctaagt taaagaaacc agtgaatgaa
    20941 tctgttcata ccagagtaga tccatttgat ccaacgaaga ttctaaaaag ataaaagtag
    21001 aaggagaacg tgatgaataa atcgcctgca gttattctta cttgtcaatt taccttgccc
    21061 aataaaaaat ctttttcaac atacattgat tatatgacaa gagaaaaagc actggaagaa
    21121 atggaacgac gtacgcccga agaagaacag gaactgcaac gaatcaagaa tgctttagag
    21181 gatttttata ttcctgaagg agaaactttt tcggaaagta agacggataa agaattcagt
    21241 gaaacgtcga atgaagctca atcgttgatg gaacatggaa tcaattttga caagttagag
    21301 gaacaagatt ttaccaaata tctttcttat atgacccgtc actatgcgtt agaacaaaag
    21361 aaagaactga cgcaaaggga acaaaaagaa taccaacggt tgaaccaagc aatgaaaaaa
    21421 tatcaaacag agccacataa aaaagcttca ggaaatcttc ctggagtttt ttctatggct
    21481 tcagacgaag tgaaaggaag tgatctgaag gagatcaaac aaatcttcca aagaggacaa
    21541 caaaaaggct caatcattta tcaagatgtt atctctcacg acaattcgta tcttgaaaaa
    21601 atgggcttgt acgatcccaa aacagatacc ttagatgaag aaggtttgaa agcagctgga
    21661 agaaaaatga tggagacatt attcgagaaa gaacaattga atgaaacagg ttactggatg
    21721 gcaacgattc accgtaacac taagcatatc catattcatt ttgcagttgt tgaaaaagaa
    21781 aatacgcgta aaccgtatac tgagattgaa aatggtgtga gttatatcgt accgaagggg
    21841 aagagaagac aagcaacatt agacgcaatg aaaaccaatt atgtacatga gttagaacgt
    21901 ttctcttatg aaaaggatcg aatgatcggt atggagaaac gaaatttatt gacacgaaaa
    21961 agtgatttgc gtaacacctt aacaaaagta gtaaaagaac cagcacgtta cgatcgacac
    22021 gccatgaact tattaaatga agtatataaa accctacctg aaaaacgttc ggaatggaat
    22081 tatggagatg aaaatcgaac caagctttca ccatttacaa ggggaaaact tgatgagtta
    22141 accaaatatg ttttaacaaa agaacctgga tatcaagaat atatacagat ttcaaaagca
    22201 ttgaaagaag aagccaaaag cctttatggg gaaagcaaaa gagaaacaaa gaatgcagaa
    22261 aaaaacgccc tctttgattt aaaaaagcgc acaggaaatg cgattttaac agaactgaag
    22321 agacaggata gtcaaataaa accattaata gaaagtgtca atcagatgca atctatttac
    22381 aatgaacgat cctttcgaac actcaaacag ttgccaacaa atgaatacaa gtatagttat
    22441 caagaattcg taaagcgtag gaaacaattc caacaaagag ttgtcacgca gcgaagcttg
    22501 cgtcaattaa atcgaacaat taatgcacat gaagaaaaat accacgcaag taaagcatat
    22561 gaagagcaac agcgaagaat ccaaatggaa caagaaagac aataggagga agagaaatga
    22621 aacaatattt agatcaatgg aaagtcatag aaggatcact aaaagaagaa cgaatcgaac
    22681 aacttcctga ttgtttagaa aaagagcatt tgtttcagat aagcgaaatg ttgagaaacg
    22741 aacagtttga tccaaatcaa tttttagttg ttgagtaccc agctactggt gtgtattgtt
    22801 gtaatcatgt gaagggtgaa aaatatttta ttattcaaga atacgaaggt aagttagcgc
    22861 cttattatac tacttgggaa ataaatgaag agggaatcaa taatttccca tgtgaaagca
    22921 ttgaagagtc tatttctctc acggaatgtt aaataaatag agacgtaaaa gagtcctgaa
    22981 cgtgattgtc caggactctt tttcaatgaa ttgttttccg ccaattcact gagtggtgcg
    23041 attcaatttt attatatcaa aagaaagtga gattccaaag tgaagggagg aagaagaaat
    23101 gactcagaca acgattggtg ttgtggttga aaaaagcagg gataatctcg tttttgtgac
    23161 agaaattcaa acaggtagag cttttgtagt cacagacaaa gctgcaaaag cgtatcaaaa
    23221 tggcaatatt ttaacgttaa atctgactac gaagatgttc gttgatgcag tagaaaatta
    23281 tccatttgtt tagacaaacg atattttaca aggaggaaac ggacatggca gaagaaaaag
    23341 aaccactaga taatagtcgg ttgggaaagt ccaaaagaaa attagtacgg ttgcaaaacg
    23401 aattaaataa tcaatttcaa aagctatttg atcaccaaaa attgacaaat gggcaaccaa
    23461 tgaatgacaa acgaaatggt cgttcatggt ttaaccagca agaaaaaata gaaaataaaa
    23521 tacaaagttt gagagaagag atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac
    23581 aagaagagct aaaggaaatg ggctataaca agtacggcgg actggatatg acgatcgata
    23641 atattccaag aatcaaagaa gaaatcgaac gctttgaaaa aggtgaatcg actttttcag
    23701 ccgccacgat ccgaaaatat caacggaagt tggaaacatt agaacagctg aaagaaaaat
    23761 cagaaaaagg aaaagaaaat atcctccctg aagtacaagc gatcattgac agcggacgag
    23821 tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa aggctatcga aaagttgctt
    23881 tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa ataccaagcc acgtcggaag
    23941 aggaaaaagc agctgttcgt gaattacttg aagaggctgc accaactgat tctctgaagg
    24001 agaaagaaaa agatcatttc tatcgagtag aattcaacga aaatgatcca gaaaaaggat
    24061 tgaaaagtta tggtggggag atcgtaacat tagaattgat cgatcaactt aaaacgttgg
    24121 ataaccaata cgctgcagaa gcaggttatt ataaattcta ttttgaagaa gtgaagaacg
    24181 gagaagtagt agatcagatc cgaatggatc taggggatgg cgtcaacagt aatcacccca
    24241 tctatttaga gttagcaaaa ttgtgcgaac aagtacctga gaaggaacaa aaacaatcgg
    24301 tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa agaaacaaaa acaaaacaaa
    24361 agggctatca aaaacgaacg gcagaagaga tcaaacaaga agttaaatcc ctttcgcaac
    24421 aggcactgga tgcggtgaaa aagcataccc attcttctga agacgtcaaa gaacttttga
    24481 actttatgtc tcgttttcca gaacgatcct ttagaaatca attattgatt gaggaacaat
    24541 ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca
    24601 aaaaagggga acaagggaac aagatctttg tcagaaaaac ggtcaaagga ttttatgaga
    24661 aaggacgtgg ctttgttcga gaaacagaag ctacaccaga agataaaaaa cgaatcgaaa
    24721 gtggtcaaat cgaagtgatc aaaaaacctt actacacgat tgaaaaagtg tttgatatca
    24781 cacaaacaca gttaaaacct gaagactatc cgaaaatctt tccgaaccgt gtctttgatt
    24841 ttcaattgga taaaaaagga cagtcagaac ttcaagctgg gattcaagcg gtagccaaca
    24901 atattggaat acaaattcga gatatgacag aaagtgaagt ctatcaaaga gaattaggac
    24961 aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga agaagaaatc gtcatgaata
    25021 ctcgaaatac tcctacgcag catttagcta caagtattca tgaattagcc catgcaagaa
    25081 tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt gcaggcagaa atgacttctt
    25141 atattgtgtg caaacatttt ggaatggaca cttcggagaa agctattcct tatattgcag
    25201 catggacgaa gaatggtcag acgttggatg ataaggaagc ggcggaacgt gggaaaatca
    25261 tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac catttctaat gaaatcaatc
    25321 aatacagaga aagagaacca gagatccaac cagcagaagt gaagcaggag cataaaaata
    25381 ctgtagaaac ggcagagaat aagaatattg tgcccgttgg ttcactttgg atcgacaaga
    25441 aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt acatttgaag gatgcggcgt
    25501 ttattgaaga tccgcagcat aatcaagtga ttttaaaagg aaaaagttat ggaaaagaga
    25561 ttcaaacggc gttaccttca gaagaatttg cgctaggaaa tccattccga ttttcacttg
    25621 aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta ttatgaacaa caattgtcac
    25681 aaaaagaacg cgtataaaac aagtaaaaaa gactatctga aaaacggata gtcttttttc
    25741 ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct tattgatcga tgtaaacaga
    25801 tcgatatagt ggattttgct cgaaacaatg gaatggctgt agtcaataaa ggacgagact
    25861 atcgcttaga agaccatgat tcatttgttt ttgatcgaag aaaacaacgt ttttattgga
    25921 acagtcaaaa tattagtggc gacattattg aattagctaa actttttttc atagacaaag
    25981 agatccaaga ctctaagcaa caatttaaag cagccactga ttttattcta aagaatgaag
    26041 acaaaacaga acgagtggaa aatcttcatt ttgaaacaga aaagtacaaa gatcaccccg
    26101 ttgattatca accactcaca gaaaaaggaa ggaattatct gaaggaagaa cgaaaactac
    26161 cagagtggct gattgattat gcagaaaaag aagggttgat tgccgaacta aagccaaaat
    26221 atgaacggca aaatttctta gttcgagacg atcgtttaga tcatgcggtc gcctttttat
    26281 ggaaagatcc acagacaaaa gagacagtag gggcgagtta tcaaggaacg ttcattgatt
    26341 atgaacgttt tggtgagaga ggcacatata agcatataga taaaaattcg acagcgaatc
    26401 atggctttaa cctaaagatc ggtgacccta agcagttaaa attttttgag agtagtattg
    26461 atttacttag ctatgctgca ctgaatcgtg atcagttaaa cgatacgtgg ttagtctcta
    26521 tggaagggtt aaaacaccat gtgatcagtc attattttgg tgaggcagta tctgaattga
    26581 gaaagaaaca agcctttcct caatccatcg agatttgtgt ggataatgac cgtgcaggac
    26641 atattttcta cgaaaaagaa caattaatgg gcgcagtcga tccatttacg aatcagaaag
    26701 tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc aaaagaatac aaaatcattt
    26761 atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc tattatggcc atacacaaga
    26821 cagaaaacaa tctgcaatta acgaaccaac tggtttccgc tcacaaagta aatgcgtttt
    26881 ttggccagca gctttctgtt aacgattcaa tagaagccat taatttgaaa gatatttgta
    26941 gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga tggtacgtac gattttgatc
    27001 gtttttatca agaaaaaggg gatattaatg cgcagattct tttctcttat aaagcagaac
    27061 aatactacaa aggctataaa aatcatgaac atgaatttat acctgaagta aaaaaggact
    27121 ggaacgatca attgaagcat gagatccagc aacaagaaat cagaaagcaa aaacgtgcca
    27181 tgttgtttca acaaggcaga caacaagaaa gggagtgaaa tcgtcatgat gaaaaccgtt
    27241 attttagcag aaaagccgtc acaagcgaaa gcttatgccg atagcttttc taaagctacc
    27301 cgaaaagatg ggtattttga gatccaagat cgattgttta caggagaaac tgtgatcacg
    27361 tatggttttg gtcatttagt cgagttagat tcgcccgata tgtacgatga aaaatggaaa
    27421 caatgggcat tagaacattt gcctattttc cccaatcaat accactatca tgttcctaaa
    27481 gataagaaaa agcagtttag tgtcgtcaaa cgacagctgc agtcagctga tacgattgtg
    27541 atcgcgactg atagcgatcg tgaaggggag ctgattgcct ggtcaatcat tcaacaagca
    27601 ggcgccaatc aaggaaaaac ctttaaaaga ctttggatca actccttaga aaaagaagcc
    27661 atttatcagg gattccaaca gttacgagac gcagaagaga cctatccaaa atttgaagaa
    27721 gtacaagcac gtcagatcgc cgattggtta attgggatga acggtagccc actgtatagt
    27781 ttactcttgc agcaaaaagg cataccagga agtttttctt taggtagagt ccaaacccca
    27841 acactctata tgatttatca attgcaggaa aagattagaa acttccaaaa agaaccatat
    27901 tttgaaggaa aagcccaagt aatcgctcaa aatggcgcat ttgatgcgaa gcttgatcca
    27961 aatgaaaccc aagcgacaca agaagcattt gaagcctatt taaaagaaaa aggtgttcaa
    28021 ttaggaaaac agccaggtac gatccttcaa gtcgaaacag agaaaaaaag tgcggctagc
    28081 ccacgtcttt tttctttatc gagccttcag tctaaaatga atcaactcat gaaagctagt
    28141 gccaaagata ctttggaagc catgcaaggg ttgtatgaag gaaaatatct aagctatccg
    28201 cgaacggata ccccttatat tacggaaggg gaatatgcgt atctgttgga tcacttagac
    28261 gaatacaagc attttttaaa agccgaagcg attccgactc ctattcatac accgaatagt
    28321 cggtatgtga ataataagaa agtccaggaa cattacgcga tcattcctac aaaaacggtt
    28381 atgacagcag cggcttttga acaactaagt ccactgcagc aggctattta tgaacaagtg
    28441 ttaaaaacaa cggtggccat gtttgcggaa aagtatacct atgaagaaac aacgatcttg
    28501 acgcaagttc agcagcttca gttaaaggca atcgaaaagg tgccgctaga tctaggttgg
    28561 aagaagttat ttggaaaaga gagcgacgga aaagagaagg aagaagagcc gctacttcct
    28621 aaagtgacaa aaggagaaat ggtgacggtg gatctacaag ttcttgaaaa agaaaccaag
    28681 ccaccacagc cttataccga aggtacgttg atcacggcga tgaaaacagc tgggaaaacc
    28741 gttgatagtg aagaagcgca atccatattg aaagaagtgg aaggaatcgg tacagaagcg
    28801 acacgggcga atattattga aaccttgaaa caaaaggaat atatcaaggt agagaaaaat
    28861 aagctcgtgg tgacgaataa agggattttg ctttgccaag ccgtggaaaa agagccgctg
    28921 cttacgagtg ccgaaatgac cgccaaatgg gaaagctact tgttaaagat cggtgaacga
    28981 aaaggaacac aaaccacttt tctagccaat atccaaaaat ttgtctctca cttattggaa
    29041 gtcgtaccag ggcaaatcca atccactgat tttggatcga cgttacaaga agtgaaagca
    29101 gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt gtccaaaatg ccaggaacaa
    29161 gaagtcttac tctatcaaaa ggtggcagca tgtacgtctg aagcctgtga tttcaaacta
    29221 tggacaacga ttgccaaaaa gaaacttaca gcgacacagc tgaaagagat cattcaaaat
    29281 agccgaacaa gccaaccagt gaaagggtta aaaggtcaga agggttcgtt tgaagcaacg
    29341 attgttttaa aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac
    29401 ttcaagaaaa gaacacgacg cactacaaaa taatggaggg ttaatcttat gcgtaataca
    29461 acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa
    29521 atgcgtgcca gcaaacgaag actgcagcaa ttgaagaacc agcagcggat tcaaaaagaa
    29581 atcgaattag atcgataaat tcctttcttg ttaatataaa actaacatgt tatagttgtg
    29641 ttagttttat attaacttta ggggaagagt aatgaaagac aaaatccaat gtatttttga
    29701 taaacataat ggcaacctat ccttgaagga tgctagaagt gaaggattat cgcctatgac
    29761 gttagatcga ttagccgaaa aggataaaat agaaaaggta gcaccaggtt tctatacctt
    29821 aaaggatgcg ttcgtggatg aattgtatct cgctcagagt atctattcca gagggatttt
    29881 ttctcatgag actgcgctcg atctttttca agtaggaaca tatatcccta aaaagatcaa
    29941 tatgatgttt cctaaaggat acaatgtatc aaaggagaaa ttagatcagt atgccattca
    30001 accgcattac actaataaag aaaattttga cctgggagtc acagaaacag aaagctttta
    30061 cggcaataaa attttagtct atgatttaga gcgaacgtta tgtgacatgt ggaatccaag
    30121 atacaaagca agcgtagagg taaaacaaga agcattaaaa gaatatatga caagtaccag
    30181 tagaaataca aataagctaa gaagatacat ggatatttta aaatcaccaa aagaaatgac
    30241 tttgtatatg ttgccacttt attgagagga gtaaagatga atgacaccga cacaatttaa
    30301 agagaaaaca agaaatttcg cagcagagaa acatttaaac ccacaaattg tccaaagata
    30361 ttatatgatg gaaaaattat tagaatgtat tgttgaatcg ccttatcatg atgattttgt
    30421 attaaaaggg ggatttctga ttggatcaaa atacgggtta gaaaaccgtt caaccgttga
    30481 tattgatacg acttatcgaa attcgaaact aacaaaagaa aagttagaaa ctgttttttc
    30541 tgaattaaca gctaatccta caaaagatgg aattcgtttt gaattaaagg gattaacaga
    30601 gacgagggaa gccgattatt atccaggttt tcaagcaaag tttattgctt atcttgaaaa
    30661 tgcacgtgta ccttttaaat tagatattac ttcaggtgat tctattattc cagcagcatt
    30721 gatgtatgaa cacaaattaa tgtttgaaga taaaacgatt catattccag cttatcctac
    30781 ggaacaaatt ctagctgaaa aattacatgc cacgtttagt tttggaaaag ataattcgcg
    30841 catgaaagat ttttatgacc tgtatatgat tccgaaatta gaacgtatcg atgagaaaaa
    30901 tttgtataag agtgttcaag gaacattcaa ggaaagaaat aatcaatcgg tgtttaagca
    30961 tttgtatgat caggaaatgc ctcaattggt gaaagatgag ggcttgagag aaaagtggaa
    31021 aagataccaa caagaaaact attttgccaa agacatttct ttcgaatcta cactaacttc
    31081 cgttgattcc ctgatgcaat caattgtaga gcaagagaag gcagaacgaa aacaaaattt
    31141 taagagatca caacaaatga atcaagaaat ggagagataa agatgatcaa taatgtaact
    31201 ttagtagggc gattaactaa agatccagat ttacgttata ccgcaagtgg cacagcagtg
    31261 gcgacttttt ccctagccgt taatcgtaat ttcacgaatc aaaacggcaa tcgagaagct
    31321 gattttatca attgcgtgat ttggcgcaaa ccagcagaaa cgatggcgac attagctaaa
    31381 aaaggcagct tgatcggcgt tgtcggacga attcaaacac ggacttatga caaccagcaa
    31441 ggacaacgtg tgtatgtgac agaagttgtg gccgataact tccaactatt agaaagtaaa
    31501 gcagcgacag aaagccgtat gcacgctgat caatcgtcca gcagtccaag ccctaccacc
    31561 tttgaacaac gggatacagc gacgcctaac aacaatggat tgaacgcttc acagaatcca
    31621 tttggtggac aatcaatcga tattagcgat gatgatctac cgttctaagt agcattttct
    31681 attggttgca tttgctacgg tttatgttat aatttagaca acaaaaagaa ttttcaatct
    31741 ataaccaaaa accactagct ggcgggctag tggctttctt ttacgtttct attataagaa
    31801 ttaacgggta ggcttactgt ctttcttggc aaatttacaa tcaagccatt tgcagatata
    31861 gtgcgcggct acttctgcca tgattgcaaa aacaaaatca aaaagaattt tcaaacctat
    31921 aacctcctcc ccttgccaga ttaggccggg gtagcagaca gcaagactat tatagcataa
    31981 ttgaaatatc caaatgattg gatttttgct ataattttag cgaggtgatt gtgatgaaca
    32041 aatatgttgg tttgcttgat aaaatccgag taataaaaac gcaaccgtta ttggttcgct
    32101 ttactttaca aacgattcat gaatcgatta attgtgtagt tgcagatatt gaaattatcg
    32161 ataaattgct aattatggat gatggaaaat acaatattgc cgtcacaggt cacttcaata
    32221 aacgaaatca gctagttatt gctagcatgt atgttagaaa tcccgatcat tttacgcgat
    32281 cgatgggcat ataaataaaa gaagagttgt agtcctaaat gaggattgca gctctttttg
    32341 ttttacatag aaacaggaaa agaccttttt aggaagacac aagtgccttc tcaattgaaa
    32401 aacctttctc tcactccaac cgccaccgcc gttcgagaag aaaggaagtg aacgctgcac
    32461 gttcactaag atatggtgtc ttgcaaagca agacaaaata gacaaaactt cctctcacca
    32521 aaaggttcgt tcctgttcgt tatctcttta ttgatttgcc agaccggcaa caagttgcct
    32581 tttatcacca aaaagcgaca aggacaaggg taaatggcac gctgcgcgcc cttgtccttg
    32641 tcgcttttcg ttgatttatt ctggccaaca aatcaataaa gaaagaacta acgaaaggaa
    32701 gtaaaacctt tcctaagagg aggcagaatc aaacatgcga aagaaatttg attattgggg
    32761 tgttcccttc agcgaattat tcccaaacta tcatgcacca catactgttg aatgcgattg
    32821 tggagaaaga gcgaaatgta tcaaaagcta tcgtttgtat caatgtccaa catgcgggaa
    32881 aaaatatacc ttgtcttatg gcgattacat tttgattgat gagaaaggaa agaaacgctg
    32941 atgaaaacac aacacaaatt attgactgta aaagaagtcg ttcaaatttt ggtggacaaa
    33001 gaagtcattt ctgaaaaagt agcagaagct ttacttattc cgcggtactg gacgatagaa
    33061 acccctttag tagaggatgc accgactatg ctatttgaag acctagtgaa gattgcagga
    33121 tatgaactgc attatcaaga aaacatggaa acttatgaaa tcgaatattg gttaaccaaa
    33181 ttagagaact aaaaaggaga aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat
    33241 tacctttgga taaaatggca caaaaaatgg aggatatgac gttcctatac catgaaacac
    33301 gtgtacccaa aaaacattac aaggaaaaat tgtcagtcgc agtggaagaa atgattgaat
    33361 caggtgtcga aatgaatctc attgcgacgt actatcgaac actggaagaa ctgaaaaaac
    33421 aaaatggaaa atggtttttc caagccttac tatgcttgga agtaggtgtc aaaccaagca
    33481 ctatcaaacc ttccgaatat caagccttag aactgactta tgcaaaattc gcacagatta
    33541 agaaagctaa agcaatggct agcgactggc tagatacttt tgaaagtatc gaaaaaaatg
    33601 gtgcatttta tacgattgaa tcggaggaga aagataatga ataaggtggt ttatcagcta
    33661 acaacagaag atgtttcgca atttttaaca agcaaagaac acttgaatga agtaatcaga
    33721 cggattcaaa aagactttag ttgtatggaa gaaatgaagt actttattca aagagtggaa
    33781 gaacagttgt atatggaaag cgaaacaagt caaacgaagg tcacagtcag ccataatcat
    33841 ttagacgtgt ataaacgtcg tcctgaaacg gtaaaaaaag gaacagagct tctacgacta
    33901 gaaacagaaa aatacatcat gaatgtgtac ctagaaagga gtgagctggt cgaaaacttt
    33961 gtcagctaca cgatttgtag gaaacactat tactcagaat cagaatatta ttcaattttc
    34021 gagagcatgg aattaatgga agaagaaatg gaagagctaa acagcaaaaa tgcgacggta
    34081 gacgattatt accttgtatt tactaggttt ttgacgtgtg ggacggaatc tatcgtgtta
    34141 atgggaatgg attatgaact cgctgtaccc gaagcatttt tgagggaata tgtagaagaa
    34201 gtagataacc caggcgacga ctggtacacg actttaactg attttttaga cacctacact
    34261 tacgacgata cagaagaaat ccaaaatgaa cttgaccgta aacgaatgcc atatcaacga
    34321 tttgaaagga aaaaaggtaa ataaccattt ataccgaaaa gtgaggatgt gtgaaataag
    34381 aacaaaaaac cttgaaatta agggaatggt tcttatttcg caccaatcgc tccaaccgcc
    34441 cccgccgctt aggtggtgcg aaataagcgg gcaaagcccg ataacctaag agaaagaagg
    34501 agatccatat gaataaaatc attcctctat tgttcgtact gtttgttggc gagatactta
    34561 tgcgtcatgc tgcagctgta catttaaact tcgtagtatt cttaggaata ctggtatttt
    34621 gtttgatctt ttggcgattt gtattagccc ttgtgagtct actattcaag atgattgcag
    34681 ccatattttt catagggtta tttcgatgaa aggagagagt actaatgcca attgcaatag
    34741 gaaataaacg gttacccgtg acattagatg aaaagagaca aaaagaattg cagcaactaa
    34801 agcagaagta cggaaaaagt gaatctaaga ttatgtgtat tgcgttagat ctattgatcg
    34861 cgcaagaaaa agcaggattt gatataccag cactaaaaaa gtgacgtcac ttttcatttg
    34921 aagaaacaaa agtgatagca cttaattgtt cctactattt agaatatttt ctatgtcctt
    34981 tttattccat tttgtatcaa taactatgaa atctttaaag tatccagaag agattatttt
    35041 tagatcaatt tttttcttac ctgattctaa agattttgtt ttcttaaaca tattatgatt
    35101 tcctttcgac tattttcata taaatgttaa catagtttat ggattcatgg aatgttaatc
    35161 taatttaata gaaaaggata attaagtata tacgtttttt cttatgtatg ttatactatg
    35221 tatatacaga aagaggtgtc tactatggaa atgttaccta taaaaaaatg gggaaatagt
    35281 aatggcctta gattgccaaa gtatattatg gaatatcttg gaattcatac agaagacaaa
    35341 gtgaagataa tacaagaaga agtaaacggt cagaagcggc taattatcga agcggctaat
    35401 atagaaaatg aattaacaat tgaacagtta tttgaaaatt acactgatga aagaaatcat
    35461 gtgacgattc aaaatttagg tgaagcagta ggtaatgaaa aatggtgaga aaacctagac
    35521 aaggcgatat tctattattg aatacagcac ctcgatctgg acacgaacag acaggtaaaa
    35581 gaccttatat tgttttaagt catgatatta ttgcggatta tagtaacgtt gttacagttg
    35641 ctccaatttc tagtacaact agaaactatc cgctatatgt aaatataaac tcagaatatg
    35701 aaatgaagac aacaggaaaa gtattattag atcaactaac gacaattgat tatgaagcca
    35761 gagagtgtat ttttttggaa aaagcgcatg atagtttagt tgaggagtta ttgatgaaag
    35821 ttagaacagt ttttcaaaaa gtaagcaaat aaaatagaaa cagcagcatg atcaactatt
    35881 cgtgattgac catgctgtct ttttttattc tatatacggt ttgtctcgta acattgtagt
    35941 cttttgcaat cttagcgatc gcaacacctt tttttagatc ctcaacgatg tttttataaa
    36001 caagacgacg ttggggatct tttgcattgg cactgtatag cttaggacgt cctttataaa
    36061 caccgttcgc ttttgcaatc ttaatgcctt gtgcctgacg tcgtttagat tcggtacgtt
    36121 cttgttcagc gatcatggcc aaaatttgaa tgatcaaatc tttaatgaat ttatccagga
    36181 gaggattgcc aattgcttcg gccatgatcg gtagacttgt gatgatcagc tgcacatttt
    36241 tcttcttcaa gtaattgact gaagcgatga tctcgtcgta atttcttcca agtcgatcaa
    36301 tagcttccac aataaaaata tcttgatctc ggacgaaatc cagtgcttct tgaaaaacaa
    36361 gtcgatgagt aatcgttgcg ccagattgtt tctcaatgaa aattctctct gcaccgaatt
    36421 ttttcatttc ttcaatctgc cgttgttcat tttgatcgat ggatgagaca cgtatgtatg
    36481 caactctcaa aatttttacc tactttctgc aactatttaa aaaacaccct aatcatacac
    36541 gagaaaacca gatttttcaa tgtcgcggtc gtgtctagtt agggtatacc ttatatagac
    36601 aaaaactaat gatggaggaa atagtatgtg tcacttttct ttttaaatgg caatattttt
    36661 tgtaaataaa tgtcaatcaa gaaaatttaa acgattatga cattaaaaat atattttatg
    36721 aattttctag tatataactt gatttatctg ttaagattta ttaagaaaac gttttcttat
    36781 ttttgatttc gaggtgatag acaatgaaaa ataagtttat tttatcttta ggtattttaa
    36841 ctattggaat tagtgtaact ggaactacaa ttcctacatt tgctgatagt acaaatgaac
    36901 caactactgt agtagataat aaccaagaat taactccttt agataggaac ggaatagcca
    36961 ttggtaaccc aactaacgac ttaactgtta ctgaaaatta tttaatagtt tctccattac
    37021 ttgctcgagc agcttctaaa acgatacgag tgacaggaac gatgattttt gataaagttg
    37081 gttggccttc tagacctcct caatcacgga attggaaaga aaaaagaaat ggttattggt
    37141 actatggaaa attgtattta caaagttata aatcagctgg aaacaattgg attgcgactt
    37201 attcaggaac cttaactaga aagtagggat tttttatgcc agaatttgtg aacacaccaa
    37261 atttttatca agatagcgat attctaagta aaattaattt accgaaagaa gaatcagctt
    37321 tacaagttca gcgaacttta caggagacac aaaaaattca aaaatcaatt tctaatctag
    37381 aagattctga agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat
    37441 gattttaaaa aaagagtgtc tagtcgacat tctttttttc taattgaaag tgttcataaa
    37501 atcacccatt tctggacact ttcttttttt gctttatttt tcaaaaaaac aattcatttc
    37561 atgttcataa acccataaca atatctatgt tcagttaaac tatatggata aagttatgtt
    37621 acaataaaat taatgaagga gtgatttttt tgaaaatagg atacgcccgg gtatctactg
    37681 gtttacaaaa tttggattta caagaagaca gcctaaataa atttggttgt gaaaaaattt
    37741 ttactgatca tatgagtggg gcaaaaagta atcgaccagg attagaaatg gccattgatt
    37801 ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg
    37861 aagatttgat ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt catagtattc
    37921 aagaaaatat tacgatggac aaatcaagtt ctacaggaca gctcatgttt catttgtttg
    37981 cggcatttgc agaatttgag cgtaatttga tcctggaacg atccgcagca ggacgagcag
    38041 ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa caagatttgg
    38101 aattactgaa gactttagtg gatagcggta cacctattaa gactattgcg gaacggtgga
    38161 acgtctcaag aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga
    38221 caaattctca ttagtttttg tcatttaatg ctgaaaattt ttgccattta tctagaaaag
    38281 tgacaatagt atgagaattt tttgtaagtg tctactgata acttattctc aaatgttgta
    38341 aggattttat tcatttgtta atattaa
//
LOCUS       DBKOIJ010000009        36470 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000009 DBKOIJ010000000
VERSION     DBKOIJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 36470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.009"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACV5NJ_000363"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACV5NJ_000363"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HHY6484490.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACV5NJ_000364"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACV5NJ_000364"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HHY6484491.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACV5NJ_000365"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACV5NJ_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HHY6484492.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACV5NJ_000366"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACV5NJ_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HHY6484493.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACV5NJ_000367"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACV5NJ_000367"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HHY6484494.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACV5NJ_000368"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACV5NJ_000368"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HHY6484495.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACV5NJ_000369"
     CDS             complement(8053..8523)
                     /locus_tag="ACV5NJ_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HHY6484496.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACV5NJ_000370"
     CDS             complement(8569..8994)
                     /locus_tag="ACV5NJ_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HHY6484497.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACV5NJ_000371"
     CDS             complement(9061..9330)
                     /locus_tag="ACV5NJ_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HHY6484498.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACV5NJ_000372"
     CDS             complement(9341..10936)
                     /locus_tag="ACV5NJ_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HHY6484499.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACV5NJ_000373"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACV5NJ_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HHY6484500.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACV5NJ_000374"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACV5NJ_000374"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HHY6484501.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACV5NJ_000375"
     CDS             15402..16376
                     /locus_tag="ACV5NJ_000375"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HHY6484502.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACV5NJ_000376"
     CDS             16546..17946
                     /locus_tag="ACV5NJ_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HHY6484503.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACV5NJ_000377"
     CDS             complement(18024..18746)
                     /locus_tag="ACV5NJ_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6484504.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACV5NJ_000378"
     CDS             complement(18825..19331)
                     /locus_tag="ACV5NJ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HHY6484505.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACV5NJ_000379"
     CDS             19454..21253
                     /locus_tag="ACV5NJ_000379"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HHY6484506.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22796)
                     /locus_tag="ACV5NJ_000380"
     CDS             complement(21321..22796)
                     /locus_tag="ACV5NJ_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcsB-like coiled-coil domain-containing protein"
                     /protein_id="HHY6484507.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTP
                     STDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPY
                     VWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQ
                     GGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(23051..23560)
                     /gene="mreD"
                     /locus_tag="ACV5NJ_000381"
     CDS             complement(23051..23560)
                     /gene="mreD"
                     /locus_tag="ACV5NJ_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HHY6484508.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23564..24418)
                     /gene="mreC"
                     /locus_tag="ACV5NJ_000382"
     CDS             complement(23564..24418)
                     /gene="mreC"
                     /locus_tag="ACV5NJ_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HHY6484509.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24573..25211)
                     /locus_tag="ACV5NJ_000383"
     CDS             complement(24573..25211)
                     /locus_tag="ACV5NJ_000383"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HHY6484510.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25212..25862)
                     /gene="rpe"
                     /locus_tag="ACV5NJ_000384"
     CDS             complement(25212..25862)
                     /gene="rpe"
                     /locus_tag="ACV5NJ_000384"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HHY6484511.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25875..26774)
                     /gene="rsgA"
                     /locus_tag="ACV5NJ_000385"
     CDS             complement(25875..26774)
                     /gene="rsgA"
                     /locus_tag="ACV5NJ_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HHY6484512.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(26939..29008)
                     /gene="pknB"
                     /locus_tag="ACV5NJ_000386"
     CDS             complement(26939..29008)
                     /gene="pknB"
                     /locus_tag="ACV5NJ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HHY6484513.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29005..29745)
                     /locus_tag="ACV5NJ_000387"
     CDS             complement(29005..29745)
                     /locus_tag="ACV5NJ_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HHY6484514.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29758..31116)
                     /gene="rsmB"
                     /locus_tag="ACV5NJ_000388"
     CDS             complement(29758..31116)
                     /gene="rsmB"
                     /locus_tag="ACV5NJ_000388"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HHY6484515.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31116..32054)
                     /gene="fmt"
                     /locus_tag="ACV5NJ_000389"
     CDS             complement(31116..32054)
                     /gene="fmt"
                     /locus_tag="ACV5NJ_000389"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HHY6484516.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32047..32538)
                     /gene="def"
                     /locus_tag="ACV5NJ_000390"
     CDS             complement(32047..32538)
                     /gene="def"
                     /locus_tag="ACV5NJ_000390"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HHY6484517.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32553..34961)
                     /gene="priA"
                     /locus_tag="ACV5NJ_000391"
     CDS             complement(32553..34961)
                     /gene="priA"
                     /locus_tag="ACV5NJ_000391"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HHY6484518.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35214..35519)
                     /gene="rpoZ"
                     /locus_tag="ACV5NJ_000392"
     CDS             complement(35214..35519)
                     /gene="rpoZ"
                     /locus_tag="ACV5NJ_000392"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HHY6484519.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35524..36138)
                     /gene="gmk"
                     /locus_tag="ACV5NJ_000393"
     CDS             complement(35524..36138)
                     /gene="gmk"
                     /locus_tag="ACV5NJ_000393"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HHY6484520.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9871 a         7974 c         6679 g        11946 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggcc ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attgtgtagc tgatgatgat tcttcagtca ttgaagattg tgtagctgat
    21961 gattcttctg ttgctgagct ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc
    22021 tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg cagcttgttg acgagcttgt
    22081 tctgctagac gttgttgttc acgaatacga gcttgctctg cttcagcttc tgctttttga
    22141 cggttcaagt cagctttctt gtcttcagca gttgcttgtt cagcagctaa tgaagttttc
    22201 agtacgttca aatcagcttg tttagataaa agatcgcctt tttgagattc caaagcagct
    22261 tggttttcag ctaattcttt tagtttagca tcgttttcag cttttttgtc ttctacagct
    22321 tttttatctt gtttttgttg ttccatcaag tcgttgtttg cttttaccat cgttgtcatt
    22381 gcttgaacgc gtccaatagc atctgctaat gaatcagcat tcaatacagc atcgatgtag
    22441 tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt
    22501 ttttcaatac gttcttgtaa atctgcaatg tcttttacta attgtgcaga ttcttgacgc
    22561 aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt tgatttcaga aacttggctt
    22621 tctaaggcat cgatttgtga ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt
    22681 tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct
    22741 acggcagtca aggtcattga acataccatt actgctgata ttaaactctt tttcactctt
    22801 cattcctccg actggcttaa tttaataaat atctaaatat atttaagtct ttcttaacga
    22861 tataaaaatt gtaccatagc agtgtgtcaa cgatataata gttatgttac aaaaatgttt
    22921 caaaacaact tttaaaaatg acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg
    22981 cgttaagtct agcatttctt aagataacaa cgattctttt tcgctttcga cctaaaaata
    23041 aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg aaaaatgaat aatacaaata
    23101 taatcatgtt caacagcagt gtaggtccta aaaaccttgt aataaaatac gtgttattta
    23161 ctactgctaa tttaaaaatc aactggacca ccatcgtaaa caattcataa cccgtgacaa
    23221 agatgatcat actgaaaaat tcagtaaaga tgttgacatg gataacgtct ttcattacat
    23281 acatcaacca tacaattaaa gggagagcta ctgcatagat accgataacg ccaatatagt
    23341 aagcgtcata gatagccccg agaacaatcg tggtaatcag taaataacgt ttttcaaacg
    23401 ccatactgca acataataat gccaatatca gaaagtgggc atttgacata taggtgcctt
    23461 tcgaccactc ccctagcatt cttgttaaat gaccatctat caacatcaaa aagaacaaaa
    23521 cgattggcag atagtacttc attgtttctt tcttcaacat ggactaatcc tccgctagcc
    23581 gttggacaat cgtcacaact gacacgtcat acatttctgc ataaggtttc acataaactt
    23641 cccgatccag cccataacta tctggtttcg ttttaataac cgtaccgatc ggcaagttag
    23701 ctggagaatt ccctccaaga ccggatgtct ggacaacatc cccttctttg atatccgtat
    23761 caccagttaa ttgggtcacc actaaggcat ggagcttttc atcatagttt ttaagcaaac
    23821 caaacgcttc gccattagcc gaagataccc gtactggaaa atgattggag ctttcattag
    23881 aggatgacag taattcgatt ttagacgaag ccgtattgac ctcgatcact cggccaatca
    23941 atcctttttg cgacatgact gccatatttg cttcgatacc gtctttagaa cccttgtcta
    24001 cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat gacatttgcc gttacttttt
    24061 cgtaactagt cagcgtctga ttcaactcaa gttctttttt caacgcttcg atttctctct
    24121 cgtaattctt gttttgctgt gaaacgtcat catagctatc gattttttct tttagccgct
    24181 cattttcggc ataagttgaa aaaagattat ggacagaatc tactccattt cgtacccacc
    24241 gtactggggc agagattgtt ttatcaacca ttgcaacact gtcattaacg atggattgga
    24301 agaaattggg ctttccttca ttcgctcgct gcgcagcggt cacacttagt atcgtcacca
    24361 cgatgataac aataaccaag gtaataataa tatttttatt aggattgaac ttcttcacaa
    24421 agacacctcg aattaattag actcttgtgt tcaattttat cactaaatgc cgtgaaacaa
    24481 aaggtaagtt gctcctctta agcttttttt tagcaaataa tagattctgt catttttagc
    24541 ctgaaagaca aattttttta tttgtttatg gatcaatctt tgctttgaat aactgcaatc
    24601 ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa attcgttact tgcataagat
    24661 gtcggatatt ctacttcttt attttccaat acatacttgc tgccttttaa tgtaagattc
    24721 gagacgggag tcaagcagca ataagctaaa tacttcatat ctggtatctg tcttactata
    24781 tgctctccag gcaaataata agacaaataa ttttgacaat ctctgataat aagctgatgg
    24841 gcaaagggtt tgaagcgagg ttccatacct aaccagagat tggctaaaag atgatccaat
    24901 cttccgcccg tagctccaat caaagtgact tccgcttgcg ggtaacgttc aaatgttttt
    24961 aagataccta actgtgtatc tgtatcgtct ttttctgcag gggaacgggt atactctttg
    25021 gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc
    25081 agatctactg ggtagcctgc ttgaagcagg tacagcgcac cacggtctat cccgacatag
    25141 gcatcgtatt tggacagcag agactgcggc cagttttgcg gattacctcc agcagctaat
    25201 aaaattttca tttagtctaa tgcctctttt aatcgttcga tttgtccgac tggattttcg
    25261 gctccataga tatatgatcc tgcaacgaag acatccgccc ctgcatcttt acagattttt
    25321 gctgtttctg gaacgattcc gccatctact tcgattgcat aagtccaatt gttctctcta
    25381 cgtagatcag cgagttccgt aattttttct actgtttctt caataaagct ttgtccgccg
    25441 aacccaggat tgacagtcat gactagtact tgatcagcca aaggcaaaac atgtttgatc
    25501 atggagactg gtgttcctgg attgataact actccagctt taactcctaa gctttttgtc
    25561 atttgaagtg cccgatgaat atgaggagtt gcttcgacgt ggacagtaat gatatctgct
    25621 ccagcttttg caaatgctgg aatgtagttt tctggatcaa cgatcatcag atggacgtct
    25681 aacggtaatt tagtaacagg acggattgcc tgaacaatat ttggccctaa tgtgatattt
    25741 gggacaaatt ggccatccat tacatctacg tggatgtaat ctgcgcctcc tttttccact
    25801 aactcaatgt ctctttgtaa attggcaaaa tctgcactca aaatggatgg tgctatcttc
    25861 attcttgttt cctcctattt tcgtttacct tctttttttg catacattgg ttttcttttt
    25921 tcgatttctt gtaagaattg caaataattg tcgtaacgtg tctgtgcgat accacctgtt
    25981 tctactctat gttttacttc gcagccaggt tctttagcat gcatgcactc tctaaatttg
    26041 caatggacag aagcttctac aaattcagga aattgtctcg gcagttctcc tgcttcaatt
    26101 gtcaaaaagt caatcgagct aaatcctggt gtatctgcta ctaacccgcc atagatagga
    26161 atcagttcca catgacgtgt tgtatgccgt cctcttccaa gtgcatcaga aatctctcct
    26221 gttgctaaat tcagttccgg tgatatttga ttcagtagcg tcgattttcc tgctccagac
    26281 tgacccataa agaccgatag ccgttctggg aagtaacgga tcagttctgt cgtatcaccc
    26341 gtatgattgg gtacaatcac cggatagcct attttagtat aggtttgctt gatctcttcg
    26401 attttttctg ggtcgtctga caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac
    26461 tcgtattcca aggtaactag aaagcggtca agtagattat aagaaaatgc aggctctacg
    26521 atacttgtga caatgattcc aagatcaaca tttgtgactg gtgggcgaac taattgattt
    26581 tttctcggca agatttccag aagatagccg tctgtttgat tttcactctc aaagatcacc
    26641 tgatctccga caagcggcgt gattttccga ttccggaaat ttcctcttcc tcttgtttga
    26701 taggtctctc cgtttgcgta tacatagtaa aaaccgctga ttgcttttct gatttgtcct
    26761 ttgataagtg ccacctccgt ttttgtttat tttaccatac gaagaggaaa gtaggtgtta
    26821 ttccatgcaa acttctcaaa gtatatggct tgaaaattga tcaaaagaga tagaaacaca
    26881 aaaaagagaa tgcaacaaat caatgtcgca ttcccttctg atatatttta ttcagcactt
    26941 attttgaact ttctgaggtg ctagctgatg aacttgtgga gctggaagat tcttcttttg
    27001 aagagttgct tgtgctactg gagctacttg agctgctaga actgtctgaa gagtcttttt
    27061 cagaactaga agacgctgga tctggtcctt tagagtaaat tacgttcaca accattcctt
    27121 tggtaattgt cgttcctgcc cctggtactg tacgaatcac tttatcttta tccaccgtgt
    27181 ttgaatattc ggaagtctca tgcccgatat attctgcacc gacactagct aaataacttt
    27241 gtgcttcgct ttttgtatag ccggagatat cattcaattt aggctctgtt ccttggctta
    27301 cgacaaaatt gatgtaagtt tcttctgcta caacttcacc cgaggctgga tcctgactga
    27361 tgatcgaacc tgatggtaca gtgtcgctat agtcataagt agcactaata ttattgatat
    27421 tgaatccttg tgtaacaagc caattacgaa cagtttcgta gttttctcct acatgactac
    27481 tcaaagtcac tgtttctggt cctttgctga ctgtgaaagt aatcgtgtct gttttaggat
    27541 cgacttctgt atcttctgat ggagattgcg aaatgatcat tccgctttct acctctgaac
    27601 tgaattcctc ttctttttga atcaagtcag cagaaaaccc taattctttc aaacttgctt
    27661 cggcatcttc ataactttta ccactgtaat catctaattt gatttttttc gtgccgctac
    27721 taatatacag agtgacttcg cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct
    27781 tcactacatt accttcttcg attttgtcat ctgcaatttc ttctgttttt gttgctactt
    27841 ctaatttcgc ttttgcgagt gtttctctag cagaagcttc agaaagatca gaaacatcag
    27901 gaacaacaac ttctcctcgc ccgccagaag caaagaatac agctgttccg attcctaaaa
    27961 cagcaagaat agctaatagg atcagccacc attttttctg tcctttcttt ttaggatcag
    28021 gcagaacttc ttcctcctct ggcaaggttt cgttttctgg cttgtctttt ggcagaggca
    28081 tagatttgaa tgaatcaggc attggtgcat cttccgtaag gggtgtgata acttttgttt
    28141 cattatccat cacatgaggc tgccattttt cttcgtttgc acgagctggt gataatactg
    28201 tagaaagatc atcagacatt tcctcggctg acttataacg gtctgcaggt tctttagccg
    28261 ttgcccgtaa aatcacgttt tccagtgctt gtggcacatt tggatcaagt gcttttatag
    28321 atggaagatc atcttgaaaa tgttttaaag caattgttac agcagattca ccatcaaacg
    28381 gtacgctacc cgtcaacatc tcataaagga tgatccctaa agcataaata tctgactgct
    28441 tcgttgccat acttcctctg gcctgctcag gggacagata atgaacagat cccagcatcg
    28501 tattcgtctg cgtgatcgat gtttcagata aagcgatagc aatcccgaaa tccgtgattt
    28561 ttacaacacc ttcattatcg atcagtacgt tttggggttt caaatctcgg tggatgattt
    28621 gatggttatg agctaaagaa atagcagaaa ggatctgctg cataatattt actgctgttt
    28681 cataaggaac aggataatgt gtttggatat atcgtttcaa gtccattcct ttgacgtatt
    28741 ccatcaccag atactgcatg ttgtcttctt caccaacatc gtaaacacta acaatgtttg
    28801 gatgaacgag ttctgttgct gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt
    28861 cattttgaaa gtcaaatcgt aagactttaa ctgctacatc acgatccaag attaaatcgt
    28921 gcgccaaaaa tacatttgcc ataccaccgc taccgatatt tccagtgatt tgataacggc
    28981 catttaattt tttgcccaac tcgatcatac actgtcctcc ttatattcga taagaagaac
    29041 ggtgatatta tccgctccgc ctgcttcatt ggcacgatca atcagctcag ttactttatg
    29101 agacaatggg ccttcttgat tgatgatcgt tcctatttct tcttcagaca gcatgttcgt
    29161 caagccatct gaacataaca gcagtcgatc ttttagttgc caaagatgag tagaaacatc
    29221 tacttcgact gttccaggca tgccgacaga acgtgttaaa atatttttcc ttggatggtt
    29281 cactgccatt tcttcgctga tttctcctga tttgactagt tcattgacta aagaatgatc
    29341 ctccgtcaat tgcatgattt tatcttcacg aatcaaataa gctctgctat ccccaacatt
    29401 ggcaatcgtg aaccagtcat ctgacagagc ggccgctacg attgttgttc ccataccgtt
    29461 atattccggc tgttcttgtc cacgctgata aatcagcata ttttcttctt gaatcgattg
    29521 aatgaaccat tgggcgattt tttcatcgtc aacagcttct tgtttttccc aagcttcgcc
    29581 tagattcgtt accgccatct gactggcaat gtctccggca cgatgaccac ccatcccgtc
    29641 tgctaataca gcaagcttga gacctgaacg gctggtaaat acactggcat agtcttgatt
    29701 tgtgttacgt cgtcttccta catctgattg atattcaatc tgcatttttt cacctcatta
    29761 ctttttgcgc aaacaacaaa tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt
    29821 caacattttc tcttcgattg atgattgtag cataggattg gtcaaaacat caatcctctc
    29881 aaaatcaggg tgtctttcca agaaagcaga aacaacgtct tggttttctt cttttaagat
    29941 cgtacaagtg ctataagtca gaataccgga agactttaaa gttggtgcgc agctttctaa
    30001 aatttccaat tggatttttg gtaaattcgc taattcatcc ggctgttttt tataacgaat
    30061 atctggtttt cgccgaagca gcccaagacc tgaacaaggc gcgtctacta aaatccgatc
    30121 aaactgctct gcttcgaact gtttactgac ttctctagca tccatctttt gagcgtagac
    30181 agcagaagag acacctagac gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg
    30241 tacatctaaa gcgaccactt ctccaccgca tgacggatct aaaaaagaag caatatgcgt
    30301 cgtcttacct ccgggtgctg cacaggcatc taagacatga tgttcaggct cgatttgcat
    30361 agacggcgct acaagcatcg aactttcatc ttggatcgtc atgacgccat cgtggaacaa
    30421 tgagctagaa gctaaatgcc cttttttcgc tactataccg tatggactga cttggctttt
    30481 ttcagctgtg atctcttctt cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg
    30541 tgtatctact cgggcactag catggctagg ctgatatagc gataaaccga gcttttcagt
    30601 ttcttcgtat ccgatttgat ccaccagtcg tttcgtcatc catagaggca ggctgatttt
    30661 gacagaaagt ctttcgatag gatcagcaat gccttgcaga tctggtgctc cttgacgctg
    30721 ataattcctc aagacaccat ttacaaattt ccctgtccca ggatttcctt ttacttttgc
    30781 gatttcgacc gcctcgttta aaatagcatg tgcagggact ttatctaaaa attctaattg
    30841 gtatacagaa agataaagaa gcgtcttgac ccagtcatcc actttttttg ctttagcgat
    30901 caatggtgca acgtaaaatt ccaatagtcg ctgatggcta atcgtaccat agatcaattc
    30961 tgtaaataaa cccacatctt tttgatttag ctcgctttgt ttgatcatct cattcaacaa
    31021 aagattcgag taggcgccac ccttgttcac tcgctctaat gtcagtaatg ccatatatcg
    31081 tactgatttt ttcatttttt tagggatttt tttcattatt tcacctgctg accaatctga
    31141 acttgctgac cgcttccgtt aaggaaatca tgtacggcct gttttccttt accagctggc
    31201 tgtaattcat tgatggctaa gacggtctgt ttaccgcaag caatccatag attttttttg
    31261 tctttccgga tgatcgtacc aggtgctttg tctgtagttt ctgccaatac ttcaaccgat
    31321 aataatttcc agcgtatgcc ctcataggtc gtgaatgcaa ttggccaagg acgcattccg
    31381 cgtacttggt tgtcaatttc ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt
    31441 gtgatgtttg gcgaaaaagt tgcttctgac tcgtcttgag gtactggttt ttgtccatcc
    31501 aaaatattgg gcaatgtttc tagcaatagc tttcttccta aaaggctaag cttttcaaac
    31561 atcgtcccta catcatcttg tttagtgatt ggtatgcttt cttgcgcata gattccgcct
    31621 gcatccattt ttttgatcat ttccatgatc gttacaccag tttctttctc cccattcatg
    31681 atggcataat gaacaggcgc acctccccga tatttaggaa gtaaagaagc atggacattg
    31741 attgccccta attttggtac ttgcaataac ttttcaggca agaattggcc aaatgccgca
    31801 gtgacgatca catcaggcac caattcaatg atttcttcca tttctggcga accggagatt
    31861 ttctctggct gcagcacacg gataccgtgc ttcagagccg cttcttttac cggtgtcggc
    31921 gtaattactc gtttccgccc aactggacgg tcgggctgtg ttaccactgc ctggatctcg
    31981 taacctgatt ctatcagccc ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat
    32041 actagcttag tcatcaagat gttcctccat atattcttca agttcttctt ccgggatctg
    32101 ctgtatcatt ttctcaatga acaagatacc gtctaagtga tcgatttcat gctggaaggc
    32161 acgggctaaa tagccaaaag ccgtcacttc tatctcttct ccgtctcgat cataataacg
    32221 cacggtcact tcatctgctc gcttgaccgt tccatagaca tgcggaatac tgagacaacc
    32281 ttctacgtca aggctttcac cttttgcttc gatgatttct gggttaatca gttcaaattt
    32341 ctcgccttca tctacttcga tcacagcaat tctcttgttt tgtcctactt gaggagcagc
    32401 gatccctatt ccgtcattcg caatcattgt ctcatataaa ttgtctaata aagcgattgt
    32461 ctcgtccgtg attacatcga ttggctgagc agttcttttt aatttatcat tagggtggat
    32521 caatatagga tagcgcataa tttggctctt tcttaaataa agtttaatgg ttcgttgtca
    32581 attgccacaa aaagaccttg tcgctgttct ttctggcttc cattcaaaat ttcatccaaa
    32641 acatgaggaa gattcggttc atgtttatat ttgatgatta gttgataata atatcggttt
    32701 ttcactctca aaatcatgct gggcgttggc cctaatagca agctttgagg gcttaacact
    32761 tcttttagct gattggcaat ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg
    32821 ctggcggtta ttttcacagt aaagtaataa ggaggatagc ctccacgatg ccgtacatgc
    32881 atttcctgct gataaaactg ctcataattt tgttttttag ctaattctat ggcgtaatgt
    32941 tgaggattga atgtttggat cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct
    33001 gaaacttggg taagcaattg gaatgtatgc tcactagaac gaaaatccgg taaattcagg
    33061 gaggtatcag cattcaacac cccaactaat gtcacttctg gaaagtccaa acctttagca
    33121 atcatttgtg tccctaataa tatatctgct tcttttgcac cgaatttttg taaaatctgt
    33181 tcgtgtgccc cttttcttcg agtggtatct acatccatac gcaaaatcct agcttgcgga
    33241 taaagttctc ggagttcctc ttctactttt tgagtacctg ttccatagta gcggatttta
    33301 ttgccgccac aattaggaca gcgattggga ataggttctt catgtccgca ataatggcag
    33361 cgcattgaac gtgtatccat atgcagtgta agagagatat cgcaattagg acatggaaga
    33421 acaaatccac agtccctaca catcacaaaa gacgaatatc ctcgacgatt gagcagtagg
    33481 acagtttgtt cttttttgtc taatcgattt tgtatttttt cctgcaaatt agcagaaaaa
    33541 gtagaggtcc gatgattctg aaactcttca cgcatatcta cgatctcaat agctggaagc
    33601 tgtgcagctg ctttggctcg gtggtttaac tgcaataact gataaacgcc tttttgagct
    33661 cgtgctcttg attctaaaga gggcgtcgcg cttcctagaa ctattgggca atgatgatat
    33721 ttactgcgcc aaattgctag atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa
    33781 cttgattcat gttcttcatc gataatgatc agcccgatat ttttcagcgg ggcaaaaata
    33841 gcagatcgtg cgcctactac tacttgcgct tcttcccgct caatcttgcg ccattcatca
    33901 tatttttcac cttgggaaag tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct
    33961 ttgaatcgct cgaccatttg cggagtcaat gcgatttcag gcactaacat aatagccgtt
    34021 ttattttctg acaaaacatc agcgattgcc tgaagatata cttctgtttt cccgctgcca
    34081 gtgattccct ccagcaaaaa gacctgatct ttttgctgct gacctgctgt gatgatttgt
    34141 tcgacagcat ttttttgttc tgcattcagt tccaatgccg tcgtttggtc aaatactcgg
    34201 tctttataag ggtcgcgata acgttctgat tctataaact ccagccagcc cttttctttt
    34261 ccttgattca agacagcagt actgattcct ttatttttga aataagcaat tggttcttct
    34321 tggctcagtt cagataaaca caaaagaagt tctttttgtt tcttggcatt ttgccgtaat
    34381 tttgcccatt cttcttcgat ttgcttttca gttaacaaag aacggaccaa acgaatcgtt
    34441 tttactttat ttcgtgtatg gacttcatat ttgatatcta ctaattgctc ttggcgccag
    34501 cgaatcaatt gagaaaggct gccttcttct tctgcttcct tccaggaaat cacttctcgc
    34561 tgatcaaaca atacctgaac ttctggtgtg tctgccaaag gatagatcaa tttatcatat
    34621 tctgctttca tcacactcgg aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta
    34681 atttttttca tataatcagc taatgaaagt aattcttcat tgaccaccgg agcaaggtct
    34741 agcaaacgaa taactgcctt caaagatgga ttagctgatt cttctgattt ttggatagcc
    34801 ataacaaatc cttggacatg acgattgcca ttaccaaaag ggacttctac tcgcatcccg
    34861 acttgcaaag ctgtttccat ttcagaggga accaaataag tgaatggctg atcggtctgc
    34921 atagtaggta cgtccacaat gacttcagct gttaatgtca tggttcgttt cctccttttt
    34981 ttgtcttttc ttatcaagcg acaaaaagtt ccttgcctat tttactagag atcgatggat
    35041 ttgtcttatc ttctgccaaa tcctaacaaa aaattaacag cagataagaa aaaaaagaac
    35101 tgtgtcaaaa agttcagaca cagccttttc taccagacaa taagtggcga atgtttgaaa
    35161 aggaaacagc tgggtttgct tttcttttta gatgttcgct ctatttttct ttattacatt
    35221 ttttgctctt cacggatacg tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga
    35281 cgttcctctt cttccatctt caaacgttca cgttttaatt caggatgtgg atcattgact
    35341 acatttcctg catcgatttc ttctaaagct tgaccgacgc tttttactga atcaaaagaa
    35401 tctagagtag gctgtgcact tgcatccaat tcatgcgcgc gtttacttgc taaaatcact
    35461 agagaatatt ttgaatttac acgatctaat agtgagtcaa ttgaaggttt taacatcata
    35521 agactacatc tcctttaaca ttttaatata tttaccgatt acgcgatcta ctcgaaaatg
    35581 ttcactggca ataatgtttt tgattcgttc aactgctagc ggcacttcat catttacaac
    35641 agcatagtcg taaagagcca tcatttcgat ttcttctttt gcaactttca ttcgttcttc
    35701 gattacttca tctgcatctg ttcctcggcc tacgatccgt gattttaatt ccgctaaatc
    35761 tggcggagtc aaaaagatga atacaccatc gggcactttt tctttgactt gttttgctcc
    35821 ttggacttca atctccaaga aaacatcttt tccttcatcc aatgtcttgt tgatatatga
    35881 caaaggagtt ccataataat tcccgacata ttctgcgtat tccagcatct ctccagcttc
    35941 gatcattgat tcaaactctt ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc
    36001 ttcacgcatt ttacgggtag tcattgagat cgaatactga aaatcattat cctcactctc
    36061 aaaaattgct tttcgtactg tgccttttcc tacaccggat ggaccagaca acacgattaa
    36121 taatccacgc tctgacatga cgacgtcctt tcaactagtc tttttgtact tatcgtttat
    36181 tttgcacttt ttttctgcaa ctttcaaggt tatctttaat tatagctaga aattggtaaa
    36241 aaagctaccc tgtaccaaga ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag
    36301 ccaaacataa acagcttctc atgtttttcg tttggttggg gttattatcg cagatgcaag
    36361 cttttgaacg ccgtgtataa gaggtcgtga agaagccgat tagtttcgag tatgaagaaa
    36421 aattttgtga aaatagtttc tcacattttt gcataaattt ggcttaatac
//
LOCUS       DBKOIJ010000010        34687 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000010 DBKOIJ010000000
VERSION     DBKOIJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.010"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            591..1280
                     /locus_tag="ACV5NJ_000394"
     CDS             591..1280
                     /locus_tag="ACV5NJ_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HHY6484521.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACV5NJ_000395"
     CDS             1267..2475
                     /locus_tag="ACV5NJ_000395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHY6484522.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     misc_binding    2514..2719
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACV5NJ_000396"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACV5NJ_000396"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HHY6484523.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACV5NJ_000397"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACV5NJ_000397"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HHY6484524.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACV5NJ_000398"
     CDS             complement(6578..7273)
                     /locus_tag="ACV5NJ_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HHY6484525.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACV5NJ_000399"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACV5NJ_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HHY6484526.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACV5NJ_000400"
     CDS             complement(8472..8663)
                     /locus_tag="ACV5NJ_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HHY6484527.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACV5NJ_000401"
     CDS             complement(8684..9574)
                     /locus_tag="ACV5NJ_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HHY6484528.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACV5NJ_000402"
     CDS             complement(9561..10328)
                     /locus_tag="ACV5NJ_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HHY6484529.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACV5NJ_000403"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACV5NJ_000403"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HHY6484530.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACV5NJ_000404"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACV5NJ_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HHY6484531.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACV5NJ_000405"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACV5NJ_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HHY6484532.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACV5NJ_000406"
     CDS             14899..15282
                     /locus_tag="ACV5NJ_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484533.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACV5NJ_000407"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACV5NJ_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HHY6484534.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACV5NJ_000408"
     CDS             complement(16226..17044)
                     /locus_tag="ACV5NJ_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HHY6484535.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACV5NJ_000409"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACV5NJ_000409"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HHY6484536.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACV5NJ_000410"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACV5NJ_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HHY6484537.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACV5NJ_000411"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACV5NJ_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HHY6484538.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACV5NJ_000412"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACV5NJ_000412"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HHY6484539.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACV5NJ_000413"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACV5NJ_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HHY6484540.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACV5NJ_000414"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACV5NJ_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HHY6484541.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACV5NJ_000415"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACV5NJ_000415"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HHY6484542.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACV5NJ_000416"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACV5NJ_000416"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HHY6484543.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACV5NJ_000417"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACV5NJ_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HHY6484544.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACV5NJ_000418"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACV5NJ_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HHY6484545.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACV5NJ_000419"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACV5NJ_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HHY6484546.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACV5NJ_000420"
     CDS             28352..30325
                     /locus_tag="ACV5NJ_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HHY6484547.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACV5NJ_000421"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACV5NJ_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HHY6484548.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACV5NJ_000422"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACV5NJ_000422"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HHY6484549.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACV5NJ_000423"
     CDS             33204..34496
                     /locus_tag="ACV5NJ_000423"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HHY6484550.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10570 a         6743 c         6857 g        10517 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaa
//
LOCUS       DBKOIJ010000011        33862 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000011 DBKOIJ010000000
VERSION     DBKOIJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.011"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..217)
                     /locus_tag="ACV5NJ_000424"
     CDS             complement(<1..217)
                     /locus_tag="ACV5NJ_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HHY6484551.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENN"
     gene            complement(214..732)
                     /gene="msrA"
                     /locus_tag="ACV5NJ_000425"
     CDS             complement(214..732)
                     /gene="msrA"
                     /locus_tag="ACV5NJ_000425"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HHY6484552.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(752..1465)
                     /locus_tag="ACV5NJ_000426"
     CDS             complement(752..1465)
                     /locus_tag="ACV5NJ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HHY6484553.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1468..2364)
                     /locus_tag="ACV5NJ_000427"
     CDS             complement(1468..2364)
                     /locus_tag="ACV5NJ_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HHY6484554.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2522..3364)
                     /locus_tag="ACV5NJ_000428"
     CDS             complement(2522..3364)
                     /locus_tag="ACV5NJ_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HHY6484555.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3496..4149
                     /gene="trhA"
                     /locus_tag="ACV5NJ_000429"
     CDS             3496..4149
                     /gene="trhA"
                     /locus_tag="ACV5NJ_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HHY6484556.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4212..4739)
                     /locus_tag="ACV5NJ_000430"
     CDS             complement(4212..4739)
                     /locus_tag="ACV5NJ_000430"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HHY6484557.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4759..5706)
                     /locus_tag="ACV5NJ_000431"
     CDS             complement(4759..5706)
                     /locus_tag="ACV5NJ_000431"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HHY6484558.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5718..7601)
                     /locus_tag="ACV5NJ_000432"
     CDS             complement(5718..7601)
                     /locus_tag="ACV5NJ_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HHY6484559.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(7764..9842)
                     /locus_tag="ACV5NJ_000433"
     CDS             complement(7764..9842)
                     /locus_tag="ACV5NJ_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HHY6484560.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(9855..10130)
                     /locus_tag="ACV5NJ_000434"
     CDS             complement(9855..10130)
                     /locus_tag="ACV5NJ_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484561.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(10492..10947)
                     /locus_tag="ACV5NJ_000435"
     CDS             complement(10492..10947)
                     /locus_tag="ACV5NJ_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484562.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(11101..12309)
                     /locus_tag="ACV5NJ_000436"
     CDS             complement(11101..12309)
                     /locus_tag="ACV5NJ_000436"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HHY6484563.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(12421..12753)
                     /locus_tag="ACV5NJ_000437"
     CDS             complement(12421..12753)
                     /locus_tag="ACV5NJ_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HHY6484564.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            12901..13776
                     /locus_tag="ACV5NJ_000438"
     CDS             12901..13776
                     /locus_tag="ACV5NJ_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HHY6484565.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(13858..14439)
                     /locus_tag="ACV5NJ_000439"
     CDS             complement(13858..14439)
                     /locus_tag="ACV5NJ_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HHY6484566.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(14445..15704)
                     /locus_tag="ACV5NJ_000440"
     CDS             complement(14445..15704)
                     /locus_tag="ACV5NJ_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HHY6484567.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(15706..16752)
                     /locus_tag="ACV5NJ_000441"
     CDS             complement(15706..16752)
                     /locus_tag="ACV5NJ_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484568.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(16987..17262)
                     /locus_tag="ACV5NJ_000442"
     CDS             complement(16987..17262)
                     /locus_tag="ACV5NJ_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HHY6484569.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(17505..18815)
                     /gene="der"
                     /locus_tag="ACV5NJ_000443"
     CDS             complement(17505..18815)
                     /gene="der"
                     /locus_tag="ACV5NJ_000443"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HHY6484570.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(18997..20226)
                     /gene="rpsA"
                     /locus_tag="ACV5NJ_000444"
     CDS             complement(18997..20226)
                     /gene="rpsA"
                     /locus_tag="ACV5NJ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HHY6484571.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(20361..21041)
                     /gene="cmk"
                     /locus_tag="ACV5NJ_000445"
     CDS             complement(20361..21041)
                     /gene="cmk"
                     /locus_tag="ACV5NJ_000445"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HHY6484572.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(21090..21725)
                     /locus_tag="ACV5NJ_000446"
     CDS             complement(21090..21725)
                     /locus_tag="ACV5NJ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HHY6484573.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(21791..23194)
                     /locus_tag="ACV5NJ_000447"
     CDS             complement(21791..23194)
                     /locus_tag="ACV5NJ_000447"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HHY6484574.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(23181..24212)
                     /locus_tag="ACV5NJ_000448"
     CDS             complement(23181..24212)
                     /locus_tag="ACV5NJ_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6484575.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            24243..24464
                     /locus_tag="ACV5NJ_000449"
     CDS             24243..24464
                     /locus_tag="ACV5NJ_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HHY6484576.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(24629..25366)
                     /locus_tag="ACV5NJ_000450"
     CDS             complement(24629..25366)
                     /locus_tag="ACV5NJ_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HHY6484577.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(25519..26859)
                     /locus_tag="ACV5NJ_000451"
     CDS             complement(25519..26859)
                     /locus_tag="ACV5NJ_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HHY6484578.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(27096..28619)
                     /locus_tag="ACV5NJ_000452"
     CDS             complement(27096..28619)
                     /locus_tag="ACV5NJ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HHY6484579.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(28997..29608)
                     /locus_tag="ACV5NJ_000453"
     CDS             complement(28997..29608)
                     /locus_tag="ACV5NJ_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHY6484580.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(29731..29856)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(29938..30654)
                     /locus_tag="ACV5NJ_000454"
     CDS             complement(29938..30654)
                     /locus_tag="ACV5NJ_000454"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HHY6484581.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(30660..31229)
                     /gene="scpB"
                     /locus_tag="ACV5NJ_000455"
     CDS             complement(30660..31229)
                     /gene="scpB"
                     /locus_tag="ACV5NJ_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HHY6484582.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(31213..31983)
                     /locus_tag="ACV5NJ_000456"
     CDS             complement(31213..31983)
                     /locus_tag="ACV5NJ_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HHY6484583.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(31980..32351)
                     /locus_tag="ACV5NJ_000457"
     CDS             complement(31980..32351)
                     /locus_tag="ACV5NJ_000457"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6484584.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(32370..33257)
                     /gene="xerD"
                     /locus_tag="ACV5NJ_000458"
     CDS             complement(32370..33257)
                     /gene="xerD"
                     /locus_tag="ACV5NJ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HHY6484585.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(33353..33826)
                     /locus_tag="ACV5NJ_000459"
     CDS             complement(33353..33826)
                     /locus_tag="ACV5NJ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HHY6484586.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT         9446 a         7177 c         5740 g        11499 t
ORIGIN      
        1 gattattttc taaatatttt tcccatgctt cgtcaaagat atccatggac ggcaaatagt
       61 cgacattcag ttccagataa ttagaaattt cttcgtagct agagctgtgc ttaggaaatt
      121 ggatgtcttt accggcttct gtagcgaaaa cagataaagc atcatgtgtg ccaccgcctc
      181 gcagtgtcat taaataatga aaaaagcttc gattcaccct tcgttcctcc aattttcttc
      241 gataaaggca gcccggcgtg catggtttct tttaaaattt tcaggatgct ttttataata
      301 ctcttgatgg tcttcttctg ctagataaaa tggttgggct ggttcgattt ttgtaacgat
      361 tggctgttcg aaacgtccac tttcagctaa ctgtttctta cttttttcag caatgatccg
      421 ctgttcttct gtcaaataaa agatcaccgg gcgataattg tcaccgcgat cctcgaattg
      481 tccgaaagcg tcagttggat cagtttgctg ccagtaaagt tctactaatt gttcatatga
      541 aatcaatgtt ggatcataga taatctccac tgcttctgta tgtccagtcg tttggctttt
      601 tacttgttca taagtcggat ctggtacatg ccctccagta tatcctgaca tcactgtatg
      661 gataccagga agcgtgtcga aaggctggat catacaccag aagcagcctc ctgcaaatat
      721 tgctcgttcc attatgattc cccctctaat actactcatc cgatgatttt ttattttctt
      781 ttgtcttgct ttcagtactg ctggtgctag ttttgttggt atttgagttg tttgaagcat
      841 cagaggtatc ttttggcaaa tatagactaa accgaatgtc atcatttact aagtcgattt
      901 tatctgcttt aatatacatc ccgttttgca ttcggaattg atccaaccta agcatgatag
      961 tcagatcatc tgtattgact tcgatccaat ctggtaattt atagtttctc ttgatcatat
     1021 tcatcacatc tttgattggc aaactcaatg ttccgataga aagacttcta gcttttagtt
     1081 ggacatttcc gttttccatc acatacggat caaaatataa atagaaatcg ataggaaaac
     1141 ctagtacttc gaaagtacca ttgagtagtg cttcattttc caaataaaat ttatattcaa
     1201 tatcactatc tttttgatac tcactaagga aaaagctgat gatttgattg actttttctt
     1261 tatttgaatt gatcgttaag acaggatctc cttgtctttc cgtaagagct ggtatttctg
     1321 ggagttctgg ttcccgatta gcaaaaatac ggctgcctaa aaatacggtc gaaccaagta
     1381 ctaccccaag aaggataagg aaggcgaatt tccagtaatt tgttttcttt gttttgtttg
     1441 aagatacgtc agatcttgtt ttcatgttta ttgatcatcc tccttctcta accattggtt
     1501 ttgtgtctcg atcagctgat ctctcactgc atttgccatc aattgataac ctaaattatt
     1561 tggatggaaa tgatcttctt catagagcac attatttttg atgccattta gattttctgt
     1621 cgtgctagtt gtttgatctg ttccttcttc tgtgacacct acttcctgat tcaatccttg
     1681 ataaagcaaa tcattgattg ggataaagta gcagttcggc attttttttg tcaccgtttc
     1741 tgttcctttg ttccaattgt taacaattgt ctgcatgtca gtgatctcag gaaagttcaa
     1801 ataaaatgga ttgtagatgc ctaaaatgta aataggtgcg gttttattga gggatcgaaa
     1861 ttcttccaat agttctgtga cattttcctg atagtttttc agtggctttt taaaggaatt
     1921 gaccgttaac ccgaataaat tattttggat aactttcatc aaatcattac cgccaacagt
     1981 cagtgtgata aaatcagctg ttttgatatt gtttcgaatt tcttcttttg tttttactcg
     2041 ctttaagatc tggtcacttc gttcaccatt tacaccatag ttttctgttt caaccgcggt
     2101 caaattataa cggtctttta gatcatttgc gacaagggga acgaatcctc cttgcttggt
     2161 ttgatctcct actccttcag tcagcgaatc tccgatagct atgtagtgaa gtttttcttt
     2221 tttatttgga tcttctctca cagtagttac tggtttcatt agtgggccag ctttcggtat
     2281 agaagcagct aaacctgcat agatgatgat cgcaattaat aatgggaaca aaacgaggat
     2341 caaatgtcgt ttcgttaatt tcattttttc acctcttgta ttcgattggt tctaatgtta
     2401 tcaaaaattt tatcaaaaag gtagtcttta tgaaaaaaga aaacaattat tttcctaatt
     2461 ttataaagaa aaagagaata agctgcaagc ctttcaggct gagggcttat cctcttttcg
     2521 actaatctac ataatacatg attgcaaacg cgtctttacc agtatgcgta gcgataaccg
     2581 gattcgtatg cagtacaggg atatgcatgt ccgggaataa ttcttgcaaa ccctctttga
     2641 attcttgcgc caattctttt gcatccgcat gtgaaatacc gatttgtcgc acatttggca
     2701 attctttcag ttcatttttc aaattattaa accatttatc gaacgttttc tttcctcgcc
     2761 ccttagttac agggattaat tcagaatggg cgaaattcat caccacgcgt atgttcaaaa
     2821 cactagacag caaaccagtt gttcggctga ttcttccacc ttttaccaag ttatctagtg
     2881 tggatattcc gatgtataat tttgtattgt cttgtacttt tttgacttct tcaaggattt
     2941 tctctactgt tttaccttct tttgctaatt cagctgctcg aattacctga aaagataatc
     3001 cttggtcagt ggtatcactg tcgataaccg ttactttcgt ttttgttaga ttactggctt
     3061 gtcttgctgc ttctactgtt ccactcaagc cttttgtcat atggatcgaa atcacttcac
     3121 tgccgtcttc tcctagctca tcatacatcg agatgaattc tccaattggt ggttggcttg
     3181 ttttgggcag attttttgct ttcgccatca tttccataaa tctttcgccg tctagttggt
     3241 catcatcggg atatactgtt ccgtcgatca tgatggatag cggcatgaca cgaatgccta
     3301 aagcatcgcg tacatttttt tccatcgtac atgaagaatc tgttacgatt tttacatttg
     3361 tcattatttt ctctctctct ttctttaaca agatgcctta ttcatgttaa actacatgta
     3421 agacaatttc tatttttagt ataacagaca aataatgatt tttcattaat tacacctagt
     3481 aaaggagccg ttttcatgga aaaaacgaaa tttaatcgaa cgtatgaaat attaaatgaa
     3541 gtgtttaatg cagtcaccca tggaattggg actggattga gtatcgcggg actagtggtt
     3601 cttttagtaa aaggtgccca cttgcattcc tccctccata ttgtctctta tgctatctat
     3661 ggatcgatga tgattctttt atttttaacg tctactttgt ttcattcatt gatttttaca
     3721 aaagcaaaaa aggtgttcca agtattcgat catagttcga tttttctttt gattgcagga
     3781 agttatactc ctttttgttt gctgagtatc cgaggatggc taggttggac tttatttgta
     3841 ttaatctggt tgtttgcgat tcttggcatt atatataaag cgttgacgtt gcataaacgg
     3901 gagagagtat caaaagtatc tacgatcatc tatattttta tggggtggct ctgtgtcgtg
     3961 gctgctgttc ctctttatca ctctcttgga atgactggtg ttattttgat ggtagcaggc
     4021 gggctttctt acacgatagg cgcattcttt tatagcttga attctatccg ctatatgcat
     4081 gttgtttggc atttgtttgt tatgttaggt gcaggtttta tgtacttctc tgttttattc
     4141 tttacttaat aaaaaaaata aagctatgga tgggacagct ctaaataaca gctagtctat
     4201 ccatagcttt tttatatcgt caattcgcct gtcccgcttt ttacaggatt ctcattacgc
     4261 tgatacgttt caaattgatg cggataacga tttttttcat ccatttctcc ttgactagta
     4321 ttgatcagtg accaatcaga ccagtcaaca ggtggaaaat aagcatctcc atcgaataca
     4381 tgatggatga ctgtacggta taatatcgta caaaatggca gaaattcttg ataaacagct
     4441 gaccctccag cgatgaaaaa gataccgtct gtttcttttt ccagagcaag cacgtcttcc
     4501 acactatgca tgacatgcac gccttctgct ttataggaag aatctctcgt caaaacaatc
     4561 gtctgacgat tcggcaacgg acggctaccc atcccttcga atgtttttcg tcccatcact
     4621 agtgtatttg cttctgtcat ttgtttgaag aatttcagat catttggcaa acgccaagga
     4681 agacggtctt cttttccaat caatccgttt tcatcctgtg cccaaattgc agctaacatt
     4741 ttctcatcct ttctgattct aaacagcgat aggagctttg atcgccggat gtgggtcata
     4801 tccttctaat tggatatctt ccatctcaaa atcaaatact gactgctttt cctgattcaa
     4861 aactaaagtc ggaaatgcgc gtatttctcg gctcaattgt tctttcattt gttcgatatg
     4921 attgatataa agatgagcat ctcctaatgt gtgaacaaat tcgccaaccg ctaaacctgt
     4981 ttcatgtgca attaggtgag tcagcaacgc ataacttgca atgttgaacg gaacgcctag
     5041 aaacacatcc ccactgcgtt gatacaattg gcagcttagc cgtccctctt gaacataaaa
     5101 ttgaaacatc gtatgacaag gaggcaaagc catgctagga acatcttccg gattccatgc
     5161 agaaacgatc aatcttctag aatcaggagt ctttttgatt gcttcgatca cttgtttgat
     5221 ttgatcgata aagccaccgt cttttgtttc ccagtgacgc cattgatagc cgtagatgtg
     5281 tccaagatcg ccgtatgttt cagcaaagga atcatcggtt aggatcttct cgcaaaaaag
     5341 cttgctctgc tttttatatt cttcattgaa ttcaggatct gtcaaacatc tatggccaaa
     5401 atcagtcata tctggtccat tatagtctgg acttttgatg tagcgttcaa atgcccattc
     5461 atcccaaata tggtttttat tttgtaatag atagcggata ttcgtatctc cttttaaaaa
     5521 ccaaagcaat tcgcttttga tcaatccaaa aggtactcgt ttcgttgtta acaaaggaaa
     5581 ccctttttgc agatcaaacc gcatttggta tccgaataag cttaatgtgc ctgtgcctgt
     5641 tctgtcttct ttacgatgcc cttcttccaa caattttctt cctaagtcaa gatacgcttg
     5701 ttccatcctt tttcctccta atcttctacc cattcactca agtattccca ccgtgccatt
     5761 ttttcttcaa gttgtgcttc tgtttcagat acgtcgtttt gcaattcttg taatcgagtg
     5821 aaattatctc cttgatgatt catctcttct tggagcaatg agattttttc ttcaagttct
     5881 gcaatctcat cttcaatcgt ttcccattct ttttgttcca tgtaagataa tttcttcttt
     5941 tcagcaggtt ccttgctctg tatttttggt ttcgcttttt cagcaggctg ctgggtctct
     6001 ttttgcttca gtaaataact ggacatgttt ccgtaataaa gttcttgttt tccgtttcct
     6061 tggaatacta ataacttatc tgctaccttg tccaagaaat aacggtcatg ggaaacagtg
     6121 atcacagcac ctggaaaact tctaaaataa tcttccaaaa tcgtcaatgt tgctatgtcc
     6181 aaatcattgg tcggttcatc caataataaa acattaggtt gctgaatcag cagtttcaac
     6241 aaaaataacc ggcgtttttc tccgcctgaa agtttgccta tcagtgtccc atgcataaat
     6301 cttggaaata aaaatcgttc aagtaattct gctactccga tttgactgcc atctgctgtt
     6361 tttgcttctt cagcagcttc ttgtaagtaa gcaatcatcc gtttatctgg tgccatttct
     6421 tcattttctt gtgtataata cgcaagatga accgtttcac cgatagacat tgtaccgcta
     6481 tccaggggaa ttctgccagc tagaatatta agcaatgtcg attttcccgc accattttta
     6541 ccagtaatac ctagtctttc ccgagattga atcaataaat ctaaatgttc tagcagtgtt
     6601 tggtcattta tacggtaact gccatctttg atttccagta cttttttccc taaacgttgt
     6661 gtcgctaggt tcagctctaa atcatcttct tggtttactt ggtacaagtt ctcctttaga
     6721 tcttcgaaac ggttgatccg tgcttgctgc ttggtgccac gtgcctgtac gccagccctc
     6781 atccaagcta attcctgttt ataaagccgc ttgcgctttt cttcttgttc tacttctaca
     6841 cgatcacgtt ctgctttttc taagacgtaa gcttcataat ttcctttata ctcgtataat
     6901 tttccaaatg aaagttcgaa gattcggttt gctactctat ctagaaaata acgatcgtgg
     6961 gtaaccatca atagtgcgcc acggtattgt ttcaaatagt tttccaacca ctcgatcgct
     7021 tcataatcta aatggtttgt tggttcgtct agtaatagta aatcgggttc atcaatcaac
     7081 acttgcgcca gactaactcg ttttttctga ccacctgata aagtactgat ttttttatct
     7141 aattcactga ttcctaattt ttgcaaaata atctttgcgt tggtatctgt agtccaagca
     7201 tcttctttat tcatttgttc ttctgcttta gcataacgtt tttgcacatc agggtcttct
     7261 ccgttttccc ctagttcgat caatgcttcc tcatatgctc gaacagtttg gatcaagggg
     7321 ctgtttcctt gaaagactgc ttggagaaca gttaattcat cggaaaattc agctgcttga
     7381 gaaagataac caatacggta atcagttgga taaaacactg tttgccgatc accgtcgctg
     7441 ctatcgatac cggcaataat atttaagaga cttgttttac ccgtcccgtt cgtcccaatc
     7501 aacccgatac ggtctttttc gtggaccaaa aaagaaatct ggtcaaacaa atctttttca
     7561 ccataagttt ttttgaggtc agttactttt aattctttca acatgttccc tccttatctt
     7621 ggatattgta tcataaaaat agtgaacttc aggaaaattt ttttggtatt tgttcttttt
     7681 gtgttctcaa aaaaagaaca ggaccgattc gatcctgttc tgatatgttt tgttttttat
     7741 aaaacttgaa aatttttacc tttttatttt attggactcg ttttttttga ctttgctcga
     7801 aacgcaaatc ggaaagaaaa gttatcagat ggtttgttca tcgtataaga ttctaacaaa
     7861 ttgatattcc tgcgatagct gttcgcagaa aacgtcgtga ttttttcagc aacttcgtat
     7921 tcatcaatga tttttctttc aagataataa cctcttagca tttttttcgt aaacagacta
     7981 tcttgctgga tcatccacga agaaatcaaa ttccccgagt tcatttttat tccttcaagt
     8041 ttgcgttgct ggataaaaaa ttgaaccatc gtttcatcat aaatatcagt caagttatct
     8101 aaactttcaa taatcaactg agtatttttt tgatatgtct ctctgatttc ttggatatct
     8161 tcatcactaa taaatgtctg acgatcagca tatcttcttt gccaaaacat tttcggatgg
     8221 agaacgattc gcattaacct acggacaaca atgaaccaaa agccgataaa agacaaaatc
     8281 tggcttgtga tagaatgttc gaatcgagaa agaaaacggg agtaaaaacg ataaccatta
     8341 gaagaaagct tgcccgaacg ataactttca tccaatccat cccgttcgat cgacaaaatc
     8401 agtgcttgta tttcttgtac ttcttgcttt tcatcatcgg ttaaatcttc caagtacaga
     8461 tcacgtatcc gttcttgata attttctatc actgcttcag tggctaaaaa ttcttttgta
     8521 ttacgatcaa tttcctttat ttcttttcgc aaaacttcaa cgacctcttc taaaatcatc
     8581 aaagcattca aatccgtgct tttcgcttcc tctgtttccg tcagcatagg caagacaatg
     8641 atccctatga ccaaagtcac taagatgaca cccgccgtca gaaacaaaag caaagaccgc
     8701 tggtaaaaca tcatattatt gattgaaaat ggcaaaataa aaatcgtagc caaactgact
     8761 gttcccttta caccgccaaa tgttaagatc aagatctcgt tcaactgttt tttgaacttt
     8821 ttactgccat ctttgaatac ataaaaaaga ctgatagaaa tgaatctgat cacaaaaagc
     8881 atgacagaga ttaatacgat aatcgctaat aaaagaccgt ttggatataa accgtctccc
     8941 catactggcg agaacacttg cgtcaattcg attcctaaaa atataaatac taatgcattc
     9001 agtgtaaaag caatagttgt ccatgtactg tgggacaagc tcgacaattc tgcatcaaac
     9061 acagagattt tcctaaaacc agaagcttgg agaatacctg ctgccactgc agcaatgatt
     9121 ccagatacat caaatgcctc tgccaataca taggctagaa aaggaagcaa aagttcgatc
     9181 agcagatacc ctgtgacatc ttgtgctgat gctttttcga tcaaatgaat tattttttgt
     9241 ttgaaccaga caagcaaaaa gccgatcaaa gctcccccaa tactagaaac tactagactc
     9301 atccccgcgt ttactgctga aaaagatccg gtgatcagcg caccaagagc aaactggaag
     9361 gcagtaacac cagatgcatc attcaaaaga ccttctcctt ccaaaatatg catcgccttt
     9421 ttagggatat tcactcgtcc agaaagagaa ctgactgcta ccgcatctgt cggacctaac
     9481 gctgctccaa aagcaaagca ggcagcaagt gggacacttg gcaacaggaa tgataacgtc
     9541 gcacctaccc cgacaagggt caaaatgact cctccaaatg ccaagaacaa aattgtcccg
     9601 aaatttttga gaatcgaaga aatatctgct ttttctcctt ctctaaataa caatggtgca
     9661 ataatcataa ctaagaacaa ttcaggttca aaatcgattg attgtcccca ctctgtcaga
     9721 cctaatacca ctccaaggaa aatttggatc agtggagctg gaatcattgg caaaatgcgg
     9781 ctagctatgt tcgataaggt gatcgttact gcaaatacaa taactaactc agcaaattcc
     9841 atcgtccatc ccctttattt tttattttgt gtaagcattt ttaaaaattg ttcttttttc
     9901 tttaaaattt tggtttcgac tacttcaacc tgtgtttggt tcccctctaa ttcacactgt
     9961 acacgttttg actccaaacg aagcagatca tgtttcagac gttccaactg acgatacatc
    10021 aatatatttc ttttacgcaa cttacaaagt atttgacggc ggtcatccat cgaaagggcg
    10081 ccatagcgat catgcaacag ttttttttgt tgttcaaaac tctctctcat tttccagatc
    10141 tcctatacac aaacttccat cattatagca aagttctttt tggatgaaaa atatttcatt
    10201 gtttcatcgt ttattgctag ttagaaattt ccataaattt ttcaacgcct cattctattc
    10261 caaatagtat cagatatcaa gtaaaactta agaaagataa aagtttctga caaaaggatg
    10321 tttactttcc cattcctgcc tttcttgttc ttttatcaca acaaaaaaat ggggctgcga
    10381 caaaaatctt gtcacagtcc cttattctga ataaacggtg tggaagagac agcctatcag
    10441 tagtaataag acacatctct ttcacagcct catttttgcg taaaaatcga tttattcgaa
    10501 aaattccccg tatacatctt ctacacgttg atctggattg atcacgatat acaagttacc
    10561 tgttttttcg ctgatttttg ttgattgata ttgattgtct aatccttctg ttcctttatc
    10621 tagatatggg aagtacacaa gatcaggtgt gccatttcgg taaaggattt ccattcgctc
    10681 gatatcttcg tatttcaatg cgcgattaaa catgcccatt tccagtccgc ctaggttgat
    10741 atcatgtgtc gcaactgcgt ccccttcatg gaagatttcg attttaaatc cagcacacgg
    10801 atggatttca acaaattcac tgccattgat tcgtccaaaa cttgttgtga tttgtttgat
    10861 ccaaagatcg ccgatatgac ggcggttgat cgtccaggtt tctccatttc taaaataaaa
    10921 ttttagcgct gtcatttctt tactcattaa aaagactcct ttatgatgta atttattctt
    10981 gtactacctc agtttagcac aaatttatgt aagtggtcac atcttatgtt ttttcttcta
    11041 atgacgtatc tgcccacttt aagtagccaa caaagctgag cagatacagt ctagtgtttt
    11101 ttaaatcaat ttttctttcg ataaaaaatc caaaacctgt tttttatcat tttttattct
    11161 ttgttctacg acggcttttt cagctaattg caaagcttca ctaacccact taccaggttt
    11221 ttttccagtt tcttctagca agattttacc atctactgct aattcgctta atgacttgat
    11281 aggtaaacga tcataggatt ctcctacggc atccatctga ggtgtttcgt caaaatagat
    11341 catcaattct tctacttgca gtgcatgttt tttgcctaag aagtacaatt cgaatggttc
    11401 caaagctcca gtttctcgtt gtcgtagtcc ttggacaagt gcttgaacgt ctttaatcat
    11461 ttggttagaa cacttccacg atttcaaaaa ggaacgaatc tcattttctt ttagatccag
    11521 ttggctgatc aataatgccc atgcttggct ctcttctttc aatgggacgg atggtaattc
    11581 agcaaaacgt aaaagtgctt cgccttcttc tttcaaaccg ggacagcaaa tgtagcactc
    11641 tgtttcaata aatgcatgga ttcccgcggc ccgttcattt cccaagagca tcttgatgaa
    11701 ttccacattt atacgttcta cagatatttt acctaatagc tcgtggtttt ctttgatggc
    11761 gtagaaggtt tcttcctcta atttgaaccc tagttgactg acaaaacgca acccgcgcat
    11821 cattcgtaag gcatcttcat ggaaccgctc atgaggattg cctacagctc gcaagatctg
    11881 ttcttctaga tcttgcagac catcgaacaa atcgatgatt tctccatctt ctttcaaagc
    11941 aaacgcattg atcgtaaaat ctcttctttt caaatcttct gccaacgaac gaacgaattc
    12001 gacatgatct ggtctacggt agtcctgata agttgattcc gtccgaaatg tggtgatctc
    12061 atattgttca tcattttcta ataccaacac agttccatgt tcgatcccaa tatctatcgt
    12121 ttttggaaaa agctgtttga tttcttctgg aaatgcgctt gttgcgatat ccacatcatg
    12181 gattggctgg ttcagcaaga tatcacgaac acttccacct acaaaataag cttcatatcc
    12241 tgcattctct aattttttca gaactggaat tgccttttgg tattccagag gtaattgttt
    12301 taatttcatt tttccaacct ttcctgcgtg tttcttgtcc tgagataaag actcagttgc
    12361 atggtgactg tttgacaagt ccgtttcaga gtcttcaagg ccaggaccac ttcatcttgt
    12421 ttactcgtcc ttacgctcaa agcgattatg gtctctttga ttgaattttt ccatgttttt
    12481 ttcaaaaact tcagttaagt cgatattcaa tgaattagcc atgatcatcg taacgaataa
    12541 aacatcacca agttcttctg ctaccgagtt aactggttca gaagcctttt tgcttttttc
    12601 tccataagtg tggttgactt ctcttgccaa ttcacctact tcttcagtta atcgagccat
    12661 ctgagctaat ggagagaaat accccacctt gaattgctgg atatattcat ccacttcctg
    12721 ctgcatactg tataaagaac gctttttatc catctttttc cctccaaaac tatcttacca
    12781 aaactttaac gcaaatccta gataagagca attgagaaaa tcgaatttcc atgctacatt
    12841 taggaaggaa tttctaaatg gaaattctgt gaaaatacat aattgaaagc tggaattcat
    12901 atggtcaaac gaaaaacagt aaaagaagtt ctttttatca ttataggaac aagtatttat
    12961 gcatttggtc ttgtttactt gaacatcgct aatcatttag cagaaggggg agtctcaggg
    13021 atcaccttga tcttaagagc tttatttggt attgatccag cttatacgac tttattaatc
    13081 aacatcccat tgattttact aggaggaaag atcctcggta aacgttcctt ggcctatact
    13141 gttctaggta cactttcttt gtcattcttt ttatggatct ggcaaagggt tccactagaa
    13201 atcaatttac agcatgatct actgatcgtt tctctacttg cgggtttagt tgcaggtgta
    13261 ggaagcggga tcgtctaccg catgggtgga acaacaggcg gcagtgatat tatcgctcgg
    13321 attttagaac aaaattacgg catcactatg gggcgttccc tattggcatt tgatatcatc
    13381 gtcttacttg cttcattaac ctatatcgac ttgaaacgta tgatgtatac gttgattgtt
    13441 tcgtatgttt tcagtcgtgt gattgattcg atcttagatg gcggatattc tgcaaaaggt
    13501 ctacttgtcg tctccaacaa aagcgaagag attgcaccac tactgatgac cggtctgcaa
    13561 agaggtgtga ctttcctttc tggagaaggc gcattctctg gtaattcaaa aaaaatcatc
    13621 tatatggtcg ttagctctag tgaattaact gaagctaaga ggatcgtcca cgagattgac
    13681 gaaaaagctt tcctttcaat catcaatgtg catgaagtgg aagggcaagg ctttacttat
    13741 ttgaagccac agacaagatt actgaaaaaa ctataagaaa aaagacaaag ataggctatg
    13801 aggaaagatt ccacatccag ctgacctttg tcttttttag tactatacta tttttaacta
    13861 attgttatgt tcacttaccc ggttactttt cttttgtgtc tgcaaaagga tctttcggtt
    13921 aacttcatca gataatttca aaaaagcatc cctatctcca tcttctagtg cttgatcgat
    13981 ttgagcataa agcattttta gttttgcctc ttcttctttt tcttgaaaat atcgttcaat
    14041 taattcagta tcttcttcac tgactgaaga atcccaagaa gaaaatggat tgtcttctaa
    14101 gatagataga tattctcttg tctgccaagc attttcgaat atacattcca catataaagg
    14161 ctttttccaa ttcaaacgga tttcatgaaa aatctggtct gtgtccgtaa attcttttcc
    14221 ctgcaaaaac aatttcatgg gttccccttc aacagataga tcactgatct gcagtcctcg
    14281 atccgcaaaa ttcgcccctt caacaaagtg gacattgctt aaaatcgctt catgattggc
    14341 aagataattc aagatccaaa aaacttctct tcggctaaaa gatacatgat tgaccatcca
    14401 cgttaaaaaa ttcttctttt cccgtacatc aattagcatt tttctcacct ctctgttagg
    14461 tcatctaata atgacagtgc ctccatatct cctggttcaa gtgttaaata ataagtcaat
    14521 aattcttgcg ctcttttcag ctgcccttct tctcttaaga aaaaggcata ctctttcaag
    14581 aaatctggtt catgtgatag ttcatgataa gcctgttcat agtgtacagc tgcaacggta
    14641 aagtcttcta attcattata agcatgggct aggttccatt ctgcataagg attagcagtt
    14701 tcttccatct gattgatcac ttgtatcaca tcttcatatc tttcatcatc caagtaaaga
    14761 ttacttaaag taagtaatgt ttcatcttgt ttgtctccta cttctaaagc tttcagtaaa
    14821 agttcttctg ctcgctgtct atcatgcaga cggaaagcat tttccgaggc caggtgataa
    14881 agttcgactt ggtaaggatt ttctttgatt ccttcttcta acactgtttg tgcttcttca
    14941 atcatttctt cttcttgaag agcttgtcct agatatagat ataacgattg ataatgcgga
    15001 ttaacttctc gaagttcttc cagatagttg attgcctgct gattttcttt caattgaaga
    15061 ttcgtaaaag ctagctggaa cagtcgatca tctgtccttt cttctttcag tgcttcttct
    15121 aaaggtacga tagctgcttc gaattctcct tgcatactta aggattgtcc taaacgttcc
    15181 tgcatcgaga ttcctgaaat ttcatttatc ccgcttgcca acaatgtttc ataaaccact
    15241 gctgcctctt tgaaacgatc aactgaaaaa tacaattcag ctagcgcaaa ctggatcaat
    15301 ggttcatcag gaagcaaccc agctgcttct ttcaattttg cttcactcac ttcagggatt
    15361 ccgataactt ggtacaaatc agctatcgcc aataagcttt gcggatagta atcactttgc
    15421 ttatccactt tttccaggta ttcaaaagct aagtcgatct cattatcttc gattgcaatt
    15481 tctgctaacg ggatgttcag tccgtcattt tctgggtatt ggttgatcag ttgttcaaaa
    15541 atctgttttg cttcgatcaa aaagccaata gccagcaatt cttcgcctag ttcagctaat
    15601 atatctgctg ggtcttcttt taatgctttt tctaaaaaga tttgtgcttg tgacaagtct
    15661 tcatctgata aagcttgcaa cattttttcg ctatttgtat tcatttcaat tccctcttat
    15721 ctcactgacg atttgttgaa ataccgtcat tgttctcaat aattcttttt cgtttggatg
    15781 ttggtatccg tctattatac ctattttttc taaaacaagt atttttttgc ttttttctgc
    15841 caaaaaaaga actttttcca aatactccat ctttgaaatc tgttccagcc agtggattgg
    15901 atagccaagc aagtccagcc acacaaaaaa gttttttagt tggaaatcaa aaggatatgc
    15961 ttggagattc cacacgaaat gaaacatcat tcctaaaaat tttttcatac tagcagaaag
    16021 tagatggcca ttttcggtca gataaaaagc ttgttcaaac agtttaccat aatttgacag
    16081 gttttctgcc tctaattgat aaaaatagat catttcttcg atcatcccgg caaacggccg
    16141 agtcgttaat tgctttttat ttggatagtt tttatacaga ttttgcaaaa aacgatgatc
    16201 acaaatcatt ccgcaacaga taaaagtcaa aaagtcaatc tgctttccgt ccacctgccg
    16261 ctcgctgatt gtttgatcat aaacgatgcg ttctggcaga ttgatggttt gcagcactac
    16321 acgatttggt agctgcaaaa tcgtccgctg cggtgttaat gctcggctta gagaacggat
    16381 cgatacaggg atcatccata gtgatgaatc aagggttgta tgtttttgaa aaaaacctgc
    16441 cagatccatg atcccttcat ttccaaatcc gatgataaac atggattgat tttcgggata
    16501 gcgtttgacg aaatcaagca ggttctttaa ttctgttaaa tgattgcatt gagtgttggc
    16561 acaaatatac cagtcattgt ttgcttgatt tgcaaataac gggttgatct tatctgcaaa
    16621 caaatcataa tagcgctgat tcgttaaaaa taagattggc tgatcaatgg aaagttctct
    16681 tagctgactg actagcgttt ctccgtaaaa cactttcgtc aacgcatctt ctacttgata
    16741 atcaagttcc atacgcatcc ctcctcttct tatttttatt ttaccattag taagcaaatt
    16801 ctagaaaaga taagagcaaa gctcacctca taacttgcaa ataaataaaa attaaataaa
    16861 atacatgtgt tttctatgta tgacatacgg attcggaatc tctgtctctt atttattcct
    16921 tttctgctag gaaataaaaa gaaaagcact tgtagcccta gagccacaag cgctttttca
    16981 actgtcttat ttaacagcgt cttttaatgc tttacctggt ttgaatgcag gtactttgct
    17041 agctggaatt tcgatttctt caccagtttg tgggttacga cctttgcgag ctgcgcgttc
    17101 gcgaacttca aagttaccaa aaccgattaa ttgaactttt tcgccgttag ctaaagcatc
    17161 ttgaatagtt gaaaatacag catctactgc tgcagttgca tcttttttag ttaaatcagt
    17221 agctgaagca actttttcga ttaattctgc tttgtttgcc atggatagat tcacctcctc
    17281 cacaagagat atgatagaaa tatcatttag ttaaaacttt ctgagtggtc cagaagttta
    17341 aaaagaaata ttgattaact tcaatattct gttatcacga tatcatatga accgcttaat
    17401 agcaaggttt ttaccaattt tcacatgctt tttttagtca tagaaacgta acaaacgaaa
    17461 ctgtcttttt cttttgaaaa aaaaacgtcg atatggtaaa atgcctattt tctcctacga
    17521 gggataattc ggatggacgt cccttcaaaa gtaaatgctt ttctgatttg attttctaag
    17581 aaacgttcat aagaaaaatg catcaattcc tcttcattga caaaaacgac aaatgtaggt
    17641 ggtttgacag cgacttgtgt agcataaaaa atcttcaggc gttttccttt atctgttggt
    17701 gttggattga ttgctacagc atccataatg atatcattca acacagccga agagatacga
    17761 agattctggt tcatacttac ttgttcgatc atttcaggca gacgttccaa ccgttgttta
    17821 gtcactgcag aaacaaaaac gattggagcg taatcaagat accggaattc atcacgaatc
    17881 tcttgttcga aatcacgcat tgtattggtt tctttcttta caagatccca tttattcacg
    17941 acgataataa ttccacgacc agcttcatgg gcgtaacccg ctactcgctt gtcttgttcc
    18001 cgaatccctt cttcagcatt cagtaccatc aaaacgatat cagaacggtc aatggcacgc
    18061 attgcacgca tcacgctgta tttttcagta ttttcgtata cttttccgcg tttacgcata
    18121 ccagctgtat ctatcattaa gaatttctga ccactttcag aaacgaagtg ggtgtcgatt
    18181 gcgtctcggg tcgttccttc aatatccgaa acaattacac gttcttctcc taaaatcgca
    18241 ttgatcaaag aggatttacc aacatttggg cgaccaatca aactaaactt aatcgtgtcg
    18301 tcgtcctctt cttcagtctc ttcagagaaa tacttcactg cttcatctaa aatatcacca
    18361 atccctagtc catggcttcc tgaaacagga taaggatcgc ctagccctaa ggaataaaat
    18421 tcatagatat cgtttctcat ttctggatta tctactttat tgactgctaa aatcactggt
    18481 ttattacttc tgtataaaat tttcgcaacc atctcatctg cgtctgtcac gccttcgcgt
    18541 ccgctgacta cacaaatgat tacatctgct tcttcaatcg cgatttctgc ttgatgcttg
    18601 atctgatcca taaaaggttc atcgccaaga tcaattccgc ctgtgtcgat cacgctgaat
    18661 tctcgaccta accattcacc ttttgcataa atacgatcac gagttactcc aggagtatct
    18721 tcgacaatcg agatacgctc accagcgatg cggttaaaaa tcgtagactt accgacgttc
    18781 gggcggccaa cgattgcaat tgttggattt gccatattat ttcctcctca caagtttcca
    18841 atctcactta gtttaacgaa tgcattgcca actttcaagc atagattgtc aattaaacga
    18901 agtattttta ttccttattg aaatactaat cgcttttcaa aagaaaaact gagacaaaag
    18961 cagctgcttt catctcagtc acagatacaa aatcaattat ttttcagaat catcggttgc
    19021 tttttcttct acttcttctt tcaacgcatc acctaaaatg tctcccatag taaatccagt
    19081 attttcttct ggtaattcat aagactcaac attttttggt tcttcttctg aagctggttt
    19141 ttcttctaaa gctttgatac tcaatgcaat acgatgttct tctggatgga tctctaatac
    19201 tttcactttc acttcgtcac cttcatgaag cacttcatgt ggtgtcgcaa tatgtttatg
    19261 tgaaatttga gagatatgga ctagcccttc tactccaggg aacacttcaa caaaggcacc
    19321 gaagcttgtc aaacgtttaa ctgttccgtc taatactgtg ccaactggag cttttgtatc
    19381 gatttctgac caaggtccag gctgtgtgtc tttgattgac aaggaaacac gttcttgttc
    19441 aggattgata gacaacactt tgacatttac atcatcattg acagccaata catcacttgg
    19501 ttttgctaca tgactatgtg aaatctctga gacatggact agaccgtcaa ttccgcctag
    19561 gtcgacaaat gcgccaaaat cagtaagacg tgcaacacgt ccactgagaa catctccctc
    19621 atggatagtt gaaagaattt cttttttctt ttcttctctt tcgttttcca ctaccgcttt
    19681 atgcgataag atcaaacgat tttctgatgg ttcgatttcg atgattttaa agctcaatgt
    19741 tttgccttta tagtctgaaa agtctgcaac aaaatgatct tccaccattg atgcagggac
    19801 aaaaccacgt acaccgacgt caacgactaa accgcctttg acaacatttg taacaggtgc
    19861 ttcgatgacc ttgccctctt ggaaatctct ttcgatatct tcccaaactt ttttagcatc
    19921 taaacggcgt tttgaaagta gatagctgcc gttttcttta tctttgccga tcgtactgat
    19981 aacgactaaa tctaatacat caccgacttt tactacttcg ctgatatctt ctaccggcaa
    20041 agttgataat tcttttgccg gaacgactcc ttcaactcca gccccttcga ttccgacaat
    20101 cacttgtttg tcttcaatga ctaaaacttc gcccttaaca acatcgccaa cttttacttc
    20161 ttgtacgctt ttcattgcgt cttccattga ctggttgttc tcttgatttt gatcaaattc
    20221 tgtcataaca cttgtcctcc taaccaacat tctgccgtaa gtacggtact gtttttagtt
    20281 taattgatta ttcaaaaaaa ataaagcgat ttcgttcgtt ttccttgaaa aaacaattgt
    20341 ttttcatttt ttttcttaat ttaaaaaaga agataacctt ttttcaaaac aacagctttt
    20401 attgctgcaa cgacttctgg aatcgatttg ccagtcgtat cgatacgaac agcatcctct
    20461 gcttgcttca gcggagagac gtcccttgtt gaatcataat ggtctctttc ctgtatctct
    20521 ttcgtcagtg tttcaagatc agtcggaatt cctttttctt ggttctcttt gaaacgtctt
    20581 tctgcacgct cacttacgct tgctactaaa aagattttca cttctgcatt cggtaataca
    20641 gctgtcccga tgtctcttcc gtccatgaca acgccaccgc gttcccctat ttttcgttgg
    20701 acagcaacta attcttctct gacttttcca tgtgcagaga cttcagaaac agcttttgtg
    20761 acatctggtt gacggatttc taaagtcaca tcttttgatg ctatgaatac atgctgtcca
    20821 tcttcttgtt gcgaaaaaga gataggatac tttttgatca gcgaaactag cccttcttca
    20881 tctgaaaaag atacattgtt cgtaatagct aaataagtta ctgcgcgata catggcaccg
    20941 gtatctgtat aaatataatt caattctttt gctaatatct tggcaacggt acttttccca
    21001 gatgaagcag gcccgtcgat tgcaatactg atttgtgtca tgatataact cctttttctc
    21061 agaaaaaaag ccgcaaagcg cggctattat tatttaattc ttaattcttg accagggtaa
    21121 agcataaagt tgtttggatc taagccattc aattcaaaca attgatcagt ggtgatccct
    21181 gcacgctcag ccacttgttt tggaccttcg cctgcttgaa cagtcgttgt tgaaccggct
    21241 gcagctgatg aagaaggtgt tgtttctgct gcattgctgc ttgaatcccc tgcttgataa
    21301 gttgcctcac ttgaggaagt cggtgttgtc tctgctgcaa tactgctttc tgacgttaca
    21361 cctgtatctt gagacacagt agattcttcc gcgattgtac tagattctac tgttgaagtc
    21421 tcagttgact cctcttttgt tgtacttgta gaactttcta ctactgatgt cgaagtttca
    21481 gaagaagttg ctgctgttgc attgttgctt gatccatttc tgatccagaa ataagcacct
    21541 gctggaatag cgatacataa gaccagtaaa atcaaaaata gcgaaagaaa taatgtattt
    21601 cctttctttt gttgtctctg agaacttctt gatgaaactt catcatctgt atcgtagatt
    21661 ggttgttccc atggttcttg tgtttcgtta ttttctgtag attgatgtct atcttttctg
    21721 ctcactgttt tacctcctaa actctatcgt gtgtattata ccataggaaa aagtgtcttg
    21781 tcctaaaaaa tcaatgttcc atgttttttt taaataaatt tagtaaaatc gttccccaat
    21841 gaagttgttc agtcttagaa aaaagcttcc tctttggcag aagttctgct tttgctccat
    21901 caatatcaca acaattctgc ggcttttcgg agagtttttc gccaaaataa gctaagatga
    21961 attttctgcg gcagttttct tcattaatat agcgaagcat ctgctgcagg cggaattctt
    22021 tttctttttc ctgacgtttg agccgttcca cccaattttc tggtttttca gactgctgga
    22081 tcagctccat ccattttttt tgcagctcat caaagggagc ttgctctgtg gcatattcca
    22141 aataaagctc aaaactttgt cgctgttctc tagaaagttg attaaagaaa taatggatac
    22201 gttcatcccc actttgataa aaaagaattg ctgcactttc ttcctgatct ctgcctgctc
    22261 gaccgatttc ttgtacatag ttttctagac tgtctggcaa atcataatgg accacataac
    22321 ggatatcaga tttatcgatc cccataccaa atgcattcgt agcaatgaga aactgcagct
    22381 gattttttac aaactgctgt tgaagttgtc ttctttgagc tgcatcaaga ccaccgtgat
    22441 agtatccaac tgtaaaacgt tccctgaata aatgatacaa tcgttcgact tctttttttg
    22501 ttgcacaata aatgattgcc gctccttgtg ctctttccat gaagtgttca agttcttgtt
    22561 ctttttgctg cgttttccga acaaaaagcg agatattttg ccgattgact gattgtctga
    22621 tctcttttgg tggtcgatca aatagtacat gacgtatatc tcttgctact aaatctgttg
    22681 ctgtcgccgt tagtgccagt gtgaccgggt ttcctaaaag ctttcggatt tttcctaact
    22741 gttgatattc tggacggaaa tcaactcccc attgagacac gcaatgagcc tcatctacta
    22801 catataacgc aatattttgt tttttcagat gctctaaaag agaaggatta gtcaacattt
    22861 cgggactcaa aaacaaaaat ttatattgcg atagatgacg gaggacgtat cttctttcag
    22921 attgcgaaag taaactattg aaagcagcta ctcgtttctc ccctcgtttt tgtaattgcg
    22981 tcacttgatc ttccattaga gaaagcagcg gtgacacgat gatgaccaga ccttccatta
    23041 aataccccgt caattgatag caaaggcttt ttcccgttcc tgtaggcaaa atagcgagag
    23101 tatcttctcc atcaagcaac gcctgaataa tttcttcttg gccgggacga aatgaatcaa
    23161 atccaaaata tttttttaat tcatttatta acattctgta tcgcttcctt tctcaataga
    23221 tagatttgat acaaacgaaa ttctccataa tctaattgtc catctacatt ccattcgcta
    23281 aagcgccact ctcgcacgga atccttattt ggttccaaac gttcgattgt ttctgcagag
    23341 agaatccact cgtatggaaa atcatcaaaa aacaaggccc actcaataag atgatctgtt
    23401 actgttcctc tttttaaatg acggattgcc atgatctggt caattgtctg accattcata
    23461 aacatctgct tggtgatcat catgctttga ttgaaatttt gctgtaataa tgaatcgatc
    23521 aaagcgtgcc aaagcgaatc tggccgtttt tcgatttgtg ctaacaaaag gtggatacat
    23581 ctgctttgtt gcagataaac cgctgactca tcattgtttt ttgccagctg ataaggaagc
    23641 agccctgttc gctcatttcc agataattgg ttagctaaaa aatctgctgc ttcttctggt
    23701 atctccgaaa acagctgcac caaactctca tacgcagtat caatcaaaat ttcacgtggc
    23761 aattggcttt cactaagcca tttttttagt tggaaagtat aaaaaggact tgtctctgct
    23821 ggaagatagt cttgaatacc cgttgccaga ttagaaatta cttgaacagc aaatttaatg
    23881 agccgccaac tagtttcccc tgttctgcca tagcggtcaa aacgcaaacc agtatgttct
    23941 atttgatgtt cggacaacat atcagcccct gcaggcgtta gctttgcttc attttcattt
    24001 atttcgatcc aaccttgctg cgcaattttt tgcaaaattt gataaaactt atcctgcttc
    24061 aattctggaa aactgttatg gataaacaaa agttcgtggg taaatccata aataagtact
    24121 gaagtcgttc gttttcccac aaggagttgg taaagagaac taatccgcag cttgtgtcct
    24181 gatgaaaata gcgataaaat aaaaacaatc atattattct ccttaagtta ggatggttag
    24241 aaatgtcact actatgtaaa cttgtacctg aacgctgtat tgcatgcggt ctttgtcaaa
    24301 tcgaagcgcc agatattttc gattatgatg aagaaggaat cgttttattt gcccaagaac
    24361 cagaggctca tcaacagttc gtgatgccgg aagaagaaac atatgtaaaa aaagcgtatc
    24421 aaaaatgtcc agttcgagcg attttattag aaaataaaat ataaatataa acgaaacttt
    24481 aaaaacgtac actaagttct ctaaacttga aaataagccg ggatctgttt ctcttgttcc
    24541 gtgaaatccc gacttatttt gaaagattca tttgctatct atagttaatt gactagtttt
    24601 ttcacggacc ctataggacc catttatatt atttcaactt tttcagccag ttttccaatg
    24661 tattgacgat cacagtttgc tggtctgcgt tagaaatttc tgcctctcca tctccttttt
    24721 gctcaccata gctgccaaaa cctccatgat ttccgccatt gattgataca tactctgttc
    24781 tttccggtaa atactgctta gcttcttgat aagcttcctg attcaatact ttatcttttg
    24841 tcgccgtgat cgataaaaca ggaacagctg attctgtcaa tgttcctttt ttgtcgggat
    24901 aacttgccat gaagaatacc ccttttaaat gtttgtctga atgattctta ttgacataac
    24961 gacttgccat tacgccacct aatgaatggc caccgatcac ataatcggaa tttggaaaat
    25021 cttctttgac cttatccgct ttgtttccgc ccatcactgc tagatcaagt ggcattttga
    25081 caatagcgac tgaaaagccg ttttttgcta aatcttgcgc ccaaatacta taactttctg
    25141 gctctacgag agcaccagga taaaaaataa gtaatggaga ctttccatga ttctttttct
    25201 ctccttcaaa aaataagtaa tcttctttgt tttttccttc ttttaccaac acttgcgctt
    25261 ctgacgatgg ttcgtacgta ttttgctgta tatagtaata accggctcca ccgagtaatc
    25321 ctaaaatgat gactaagcca attagtattc gtctaaatat tttcactttc tcccaccttt
    25381 tctatctgag tgaccccttt ttcttaatca agtatgatta taccttaaaa aaataagaga
    25441 gaaatgcaaa ctttccagca tcaaataaaa aaagacggaa tatccatttg aatatccgcc
    25501 ctttttgtat gtttattttt atttagcaca taaacgatac acagcttctg cataaatttc
    25561 cattgcttta tacatactac ttaattccca tcgttcattc gcctggtgca taaagtcagg
    25621 agtatcaggg aacatcgcac cgaatgccac acactggttc atcgtacgag caaaagtagc
    25681 gccgcctgat acaaacggtt cagtcatgtc tccagtaata tcgcgatatg tccccaatag
    25741 atttttcact aactcactgt ctagtggcac gtataatgaa tcaaggaagt caaattcttc
    25801 ataagtcaac ccatattttt ctgctgtttt agttaattta gctactaaat catctttctt
    25861 gaatgtaaca gggattctca tatctacacc gatttttgtt tgttctggtg tgatttccaa
    25921 acttgcaaag ttcatcgtta gctcgccgga ttgttcatct tctgttttgc ccacgacagc
    25981 ttcaccagtt gcattttctt gaactaattt tcctaagaaa ttgattgggc caaaatcaaa
    26041 tacttctgaa agagcaattg ccagacgaga aatagcattc actccttgtg ctgcatcttt
    26101 cgcgtggata cttttaccaa gaacgacgat gccttcacct tgttcttcga aatcgaattc
    26161 gtgttttttc aatgcttctt ttacttcaga aagcttttct ccagagtaag gagctgcatc
    26221 aggaacaaca ttcaacgctc ctccagcttt tacagaaaac tcgttcgttc ctggtcctgt
    26281 caagtaagct tgcaataagc ctttttctgc ataaattaaa ggaaattcag catctggcgc
    26341 aaatccttgc gtaattcctt cttctttttc gttatatttt tctaaacaac gccataaatt
    26401 ttcttcatct gtaccaaaga taaaacgaat acgtgtcttg aattccacgc cagcatcaat
    26461 caaagctttg acggcataca ttgccgcaat agaaggacct ttgtcatctt gggatccgcg
    26521 accaactaac cagccgtctt ttatagttgg atcgaatggt tttgtttccc aattattttc
    26581 gtcccctgct ggaacaacgt ccatatgaca caagataccg aataattcat ctccagagcc
    26641 aatctctgaa taaccgtaat agccttctgg atctttaaat gttctaaatc ctagcttttc
    26701 actgatttcc aatactttat ccaaagcttc aatcactttt ttaccgaagg gatgtccttg
    26761 tcctgaatct gcttcatcta gtacggaagg aattcggatc aattcgctca atgattctaa
    26821 tgcttgttca tgatgttgtt ctgtcacaaa ttttttcatg atattatcct cctaatatgt
    26881 taaaaagggt catgtcaaat tcctccgaca caacctcttc ttctcaaata atgacgtttt
    26941 aagcctgctc catcagcaag cagaatgttc ttaacaaatg atgaaagaaa aaacactcac
    27001 tgaatggagc tattgactta tccacgtgct tatcgatagc cgtttatcgg tttcttcatt
    27061 cttatgatag accttgtacc tgtatttgtc taggtttaga acattgctgc tacacctagg
    27121 atcacacaga tggctacaaa taatatcgca attaatttac cggtaaattt ccaccacact
    27181 gaaacatcga aacgagcaat tgctacagca cccattacga tagcagatgt tggtgtaatt
    27241 aagtttacta aaccagatgc agattgataa gcagtgatta ctaaatcctt accgacgccg
    27301 gcaaattcac ccataggtcc catgattccc attgttgcag cagctaagcc ggaagttgaa
    27361 ggaatcaaga atgacatagg gatatagaaa ataaatgtaa taatgatgaa tacacttgaa
    27421 gataatgaac ttaatccttg ttcgccccaa tgtaagatgg tgtctgtgat taatccattg
    27481 ttcataacca cttggatacc acgagcaaca gctactacga ttgctacacc gatcaaatca
    27541 gaagcaccag ctaaaaattc agagacaaat tcagactctt tcatgccata agtgattgct
    27601 actacgatgg acattactaa gaataacata gtaatttctg ggaagtacca gtcacctaat
    27661 ggaagcatgc ttttacctag aaccgtacct agtaccggaa ggtttgttaa ccagtttgtc
    27721 aatgattcaa aaatcgtgaa attcgcattc aaggttgtcc aaggaatcaa actgatgatc
    27781 atgataccaa aagtaataaa gaacaataca ttaacatgtt tttgtttttt cgtgattact
    27841 tcttgacgac cgattgcttc aatatcgaaa tgcttcatat cttctttacg ttggttgtat
    27901 actaatgatt ttgttggatc tttttcgatc tttgatgcat aacgatacac atatacaata
    27961 gaaatagcta acaaaataac gaacatcaag aatcgaagac caataccttc acccatcgaa
    28021 atcccaacag cttgtgaagc tacgcctgtt gcaaaaggat ttaccgttga agctagacag
    28081 cctacttgag accctactaa tacgattgct acagctacta ctgtatcaaa gcctacagcc
    28141 atcatgactg ggatcagtaa agcatagaat ggaatcgttt cttctgccat tccgtaagta
    28201 gagccgccta atgcaaataa cagcatcaat actggaataa gcattttttc ttttcctttg
    28261 aatttcttca caactgaagc aatcccggca tctagtgccc ctgttttgtt gataatacct
    28321 aaaaatccac caacaaccaa aatgaagaaa gagattggga ttgcagcagg tgtttcttta
    28381 gttagctccc ccgctggtac acctagcatc ccattgattg gcgccatgaa aatatcccac
    28441 agcccttgag gattttgttg tactcgttca tatgatccag cgacgatgtt gcctgcatcg
    28501 tctacactat agtgcccagc tggtataaac caagtaagta cagcgattac tgcaataata
    28561 ataaataaga ccgtatacgc tgaaggcatt tgaaattttt tcttttttgt ttgttccatc
    28621 ttcttcttcc tttcttttcc gttagagaca acaattttgg ctctaatgtt attttatcaa
    28681 aaagagtatc agaacaaaaa taataacacg cattttgtaa aacttttgtt caaaaagttc
    28741 gtttttccct attccctcct cttttttcag gtttttaaag ctttcttctt tttcattgta
    28801 gcgtttcctt ttttttataa acaaacattt tcgtctttta aaaggagaag aatttcacat
    28861 ttgcaaagaa ttgattgatt tttatgcata aatcaaaaaa atactcaaaa caaacaaata
    28921 aaacgtttac attattgcat gtcttgtatt tttttccaaa aaaaaagacc aagaaatttc
    28981 ttgatctttt tgatatttat ttgactgctg tatgacgttg ttcgaactga tgttgttttc
    29041 gacttaacca tggaagaagt tgcgcatgca gcactaaaaa gacagcactc acgatgaatc
    29101 ctttgatcaa gttgaaagga acgatcccaa tcagtacata gtttgctaaa gacatgccca
    29161 gcatctgtgt tgcgttcagc cctaaaaaca ttaagtaaag aggcgtaata acgaaataat
    29221 ttgcgatact cataaaaata gttagagcga tggttcctgt aatgacacct aaaaatttgt
    29281 ttcttccttt atgctgtttc tttacactaa agaaataata aatcgggaat gtaaagatcg
    29341 ttgttgataa gaaactagca gtgtctccta ccaaattatc cggagaaaaa cctgtagtca
    29401 gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc ggccgctgga ccgaataaga
    29461 acatgctgat caataccgag atatcactaa aatcgatctt caaaaaacta aaagccggca
    29521 tgatcggaaa agcaaaatac tgtaaaacta atgcgatcgc agcaaacatc gctactgcaa
    29581 ccattttctg tacacgggtg ttcttcattt gaaatcctcc tgttaggact cctcttcagc
    29641 gaactttttg acgtttctct ccaaatgaaa aaactctatt ttccagggaa aatagagtta
    29701 gagttatttg atcaagcgtc gtcaaataag ctctatcttc tttatccaga ctatactgtc
    29761 ggtatcggaa tttcaccgat tcagctattt ttttgcaaaa taggtcgtgg actataacca
    29821 ccggtcggga atttcaccct gcccctgaag acgaacctta tataattttt gtttacatat
    29881 tcattataca tatctattca aaaaacgcaa catctaacac ttataaaaag taagtgttca
    29941 ttttgagtgg ttcagcaaac tgctgacttc ttttttgttc agttcacggt aatccccagg
    30001 acgtaggcct tgtaaagtca gatctccgta tttttcacgt tttagttttt gcacaggaag
    30061 accaactgct tgaagcatat ttttcacttg atgattacgt ccttcgtgga tcgtcaattc
    30121 cacgatactg tggtttgtct taggatccac tgataaaatt tggaatttag ctggagatgt
    30181 ttttcgtcct tcgatcttta ttccttttgt caaaggtgca agcatcgatt ttgtagcgat
    30241 cccttttacc tttgcaacgt aaactttatc cacttcatgt tttggatgtg tcaggcgctg
    30301 agcaaaatct ccatcatttg tcagtagcaa gatccccgaa gtatcgtaat ccagtcgacc
    30361 tacaggatag atacgttctt ttaccatcgg caaaagatcc gtaacaactt ttcttccttt
    30421 gtcatctgca acagcagaaa taactccttt aggtttgtac aataaatagt aaccgtattc
    30481 ttcttgataa attgggactt catccacttc cacagtgtca tgtttcccaa cttttatccc
    30541 taattccctt acaacttgtc cgtttacttt tacacgtcct gcaaggatga attcttctgc
    30601 ttttcgacga gaagtgatgc cagcgtgggc aattactttc tgcaatctct ccattacaat
    30661 tattctcctt tattcattcc atcaaagaat agatctggtg acacgtcttc ttcaagtgaa
    30721 atttccattt cttgtgtatc aggcagttct tctaatgatt tcaaaccaaa ataatctaag
    30781 aagtagccag ttgttccata taatatcggc cgacctggtc cttctactct acctttttct
    30841 tcaatcagtt tatgggcagc tagacgctgt accgctcctg aaacctgaac tcctcgaatc
    30901 tgctcgattt cagctcttgt gattggctgt ttataagcga tgattgctaa ggtttcgacg
    30961 gcggcttgag ataatgtgtt tgatgtccct tgcgcatatc gcttaagtaa gggagccagc
    31021 tcttttttag tcgttaatac ataatggctt tccgtttcaa aaatatgcaa agctgagtgc
    31081 gagttttgag tgtaagattc ttttaattct ttcactaaac ttgtgacttc tgcttcgctg
    31141 agatttaaca gtccgcttaa ttcttcatta ctgatccctt cctcgccaac aataaaaaga
    31201 agggcttcaa gttcacttaa tttcgtcatg ttcggtatct cctactgctg gataaagcat
    31261 aattgattca tagttttctt tttgttgcgc aatgatctgc ccttttttca tcaattctag
    31321 caaagccata aaagtagtga caacttcact tttagaaggt gactcaaaaa aagaatcgaa
    31381 agtacagcca tgcgcatttc tttttatacg ctcttcgata aataccattc gttcttcgat
    31441 cgttgtgtca tcgggcgtga tcgttttttc cactttttga gaaaatttct tacgttctaa
    31501 gattttctgc atggcacgga tcaaatcgat ttttttgact tgtcctcgct tcaacggctt
    31561 ttcagcatcc gctaacccat cgatgttcat aggttctttt gtgtaataaa gacttcgttc
    31621 ctctgctttt tctgtcagtc tttcagctgc atatttatat ttacggtatt ctaaaagctg
    31681 agtgaccaat tcttctcgag gatcttcttc ccataattct tcatcgtctg tgacgaattc
    31741 ttggacagga agcaatgtct tgcttttgat tgccatcaat gtagcagcca tcactaagta
    31801 ttctcctgca acagctagct cgagtgtttt cattgtatgg atataattca tatattgctc
    31861 cgtgatcgta gcgatcggta tatcatatat atcgatttcc atttgctgaa tcagatgaag
    31921 cagcagatcg agaggaccct cgaatacatc taatttaata ttgatctccg tcaactcatt
    31981 cactctttct tctttttctt tgctagccat tttccaatgt aaggctctgt gatttcagtt
    32041 gcacggatgg agacatccgg gtacttttcc tgtaattcat ctaacatttg aaagcccata
    32101 ttctcaccgc gataagacgg attcaaagaa atgtcatgga tgatcagctg atcgttcgtt
    32161 gcccagtaaa taccaataat cccttgaatg ttggttgaac cttctggcgt aaagaaaaaa
    32221 agttcataat cgtcttcaga ttcgtacgta tcaatctcct tcaacaaaga agttttacct
    32281 gtcagtttct tatggaaact caatagtccc atagcaattt tacggttttc tttccggtaa
    32341 tgtgttaaca tcttctcacc ttcctatgac taagcccttg gaaaatgctg cttataaaca
    32401 tccgtcattc ttttcttcgt gatatgtgta taaatctgag tcgtagagat atccgcgtga
    32461 cctaacaatt cctgtactgt ccgcaaatca gctccatttt ctagcagatg tgtggcaaag
    32521 ctatgccgca gcgtatgtgg tgttacttct ttgtaaatcc cagcctctcg tactaactgc
    32581 ttcagatttt tccagattcc ttgccgagaa agacctttcc catgattatt gacaaataca
    32641 tgcatttcag atgcgtcctt tacaagtaat ggacgtgctt cgtctaagta gcgttccagc
    32701 cattggatcg cataatcacc taaaggaatg atccgctctt tatctccttt tccaactgtc
    32761 tgtaccagtc caagagaaag atgaagatct cccaatttca atccaatcaa ttcactgacg
    32821 cgcatcccag tagcatacat gacttccaag attgcacgat ctcttatccc taacgttttt
    32881 gtcgtatcag gtgcttcaat cagcttttct acttctgtca gagtcaatgt acttggtaat
    32941 ttctgtgctt tcttcggcgt atcgatatgt tgcatcggat catgatcagt gatacgctct
    33001 tgccgtaaaa actgatggaa tcttctgaga cttgagatca tccgagtaac agttgctgtc
    33061 gctttttttt cttcatgcaa cttctccaga aaacggatga ctaaaaagcg atcgacatcc
    33121 tgccatgaag caattttctg ctcctgtaga aaatgcaagt actgttccaa atcacgttca
    33181 tagctttgtc gtgtattggc ggacaatccc cgttcaatat tcaagtagtg gagataatcg
    33241 ttgatttgct ctttcataat aatccccctc atgcctctct atcatatcag ataccgcttc
    33301 attctgatag tctttttcca taatctgagc aaagaaaaag ccgtctagac actcagacgg
    33361 actggtttct tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat
    33421 gatcttttac taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat
    33481 cctcttctac ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt
    33541 tggcgccttc ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga
    33601 caattttcaa ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg
    33661 gactcttttt tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt
    33721 ccaaaagcac aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta
    33781 gctgctgttt tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa
    33841 caatttataa taattacaaa ta
//
LOCUS       DBKOIJ010000012        32552 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000012 DBKOIJ010000000
VERSION     DBKOIJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.012"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            140..2749
                     /gene="clpB"
                     /locus_tag="ACV5NJ_000460"
     CDS             140..2749
                     /gene="clpB"
                     /locus_tag="ACV5NJ_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HHY6484587.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2900..3586)
                     /locus_tag="ACV5NJ_000461"
     CDS             complement(2900..3586)
                     /locus_tag="ACV5NJ_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484588.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3564..4058)
                     /locus_tag="ACV5NJ_000462"
     CDS             complement(3564..4058)
                     /locus_tag="ACV5NJ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HHY6484589.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4078..5298)
                     /locus_tag="ACV5NJ_000463"
     CDS             complement(4078..5298)
                     /locus_tag="ACV5NJ_000463"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6484590.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5445..7280
                     /gene="lepA"
                     /locus_tag="ACV5NJ_000464"
     CDS             5445..7280
                     /gene="lepA"
                     /locus_tag="ACV5NJ_000464"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HHY6484591.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7476..8387
                     /locus_tag="ACV5NJ_000465"
     CDS             7476..8387
                     /locus_tag="ACV5NJ_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6484592.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(9327..10286)
                     /locus_tag="ACV5NJ_000466"
     CDS             complement(9327..10286)
                     /locus_tag="ACV5NJ_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HHY6484593.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            10449..10700
                     /locus_tag="ACV5NJ_000467"
     CDS             10449..10700
                     /locus_tag="ACV5NJ_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484594.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            10813..11055
                     /locus_tag="ACV5NJ_000468"
                     /pseudo
     CDS             10813..11055
                     /locus_tag="ACV5NJ_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11145..11384
                     /locus_tag="ACV5NJ_000469"
     CDS             11145..11384
                     /locus_tag="ACV5NJ_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484595.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            11531..15814
                     /locus_tag="ACV5NJ_000470"
     CDS             11531..15814
                     /locus_tag="ACV5NJ_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HHY6484596.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            15913..16278
                     /locus_tag="ACV5NJ_000471"
     CDS             15913..16278
                     /locus_tag="ACV5NJ_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484597.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            16280..16732
                     /locus_tag="ACV5NJ_000472"
     CDS             16280..16732
                     /locus_tag="ACV5NJ_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484598.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            17181..18656
                     /locus_tag="ACV5NJ_000473"
     CDS             17181..18656
                     /locus_tag="ACV5NJ_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HHY6484599.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            18679..18990
                     /locus_tag="ACV5NJ_000474"
     CDS             18679..18990
                     /locus_tag="ACV5NJ_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484600.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            19351..20607
                     /locus_tag="ACV5NJ_000475"
     CDS             19351..20607
                     /locus_tag="ACV5NJ_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HHY6484601.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            20604..21149
                     /locus_tag="ACV5NJ_000476"
     CDS             20604..21149
                     /locus_tag="ACV5NJ_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484602.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            21150..21650
                     /locus_tag="ACV5NJ_000477"
     CDS             21150..21650
                     /locus_tag="ACV5NJ_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484603.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            22009..22287
                     /locus_tag="ACV5NJ_000478"
     CDS             22009..22287
                     /locus_tag="ACV5NJ_000478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484604.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            22307..22651
                     /locus_tag="ACV5NJ_000479"
     CDS             22307..22651
                     /locus_tag="ACV5NJ_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484605.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            22670..23581
                     /locus_tag="ACV5NJ_000480"
     CDS             22670..23581
                     /locus_tag="ACV5NJ_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHY6484606.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            23660..24652
                     /locus_tag="ACV5NJ_000481"
     CDS             23660..24652
                     /locus_tag="ACV5NJ_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HHY6484607.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            24649..24867
                     /locus_tag="ACV5NJ_000482"
     CDS             24649..24867
                     /locus_tag="ACV5NJ_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HHY6484608.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            24880..25290
                     /locus_tag="ACV5NJ_000483"
     CDS             24880..25290
                     /locus_tag="ACV5NJ_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HHY6484609.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            25342..27870
                     /locus_tag="ACV5NJ_000484"
     CDS             25342..27870
                     /locus_tag="ACV5NJ_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHY6484610.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            27886..30096
                     /locus_tag="ACV5NJ_000485"
     CDS             27886..30096
                     /locus_tag="ACV5NJ_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HHY6484611.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            30104..31195
                     /locus_tag="ACV5NJ_000486"
     CDS             30104..31195
                     /locus_tag="ACV5NJ_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HHY6484612.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            31216..31758
                     /locus_tag="ACV5NJ_000487"
     CDS             31216..31758
                     /locus_tag="ACV5NJ_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HHY6484613.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            31953..32210
                     /locus_tag="ACV5NJ_000488"
     CDS             31953..32210
                     /locus_tag="ACV5NJ_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484614.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            32224..>32552
                     /locus_tag="ACV5NJ_000489"
     CDS             32224..>32552
                     /locus_tag="ACV5NJ_000489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484615.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
BASE COUNT        11046 a         5116 c         6884 g         9506 t
ORIGIN      
        1 ttaagattta tataagtcga gaaaaaaagt caaaaaacta tgtgtatatt ttgaccaaaa
       61 ttgaccaaag cgatataata gtttttgtaa agatcaaaga aataagtgat cttaccttta
      121 attaggaggg tatacctgta tgaatattga gaaaatgact acgacgcttc aagaagcgat
      181 tgctgaagct caacagattg cggtcactcg aaaacatcaa gatatcgaca ttgcccatgt
      241 atggaaaatc tttttacaat ctaatcattt tggtcgaaat ttctatacgg atgccggatt
      301 ggatgtcgaa tcttttgaac atgaaattga tcgtctactg gatgagtatc cagttgtttc
      361 tggtggaaac gtccaatatg gacaaaacct cagccagaat ttctttcgat tattgaatga
      421 agcagaccaa atacgtgaaa gcttcggcga cgagtttctt tcaacggaag tcgttatttt
      481 agcgttgatg aaattaaaaa attatccgtt aactgtttat ttgaataaaa acggattatc
      541 tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag tgacctcaca
      601 aaaccaagaa gaacaataca aagcattaga aaaatacggc gttgatttag tacagcaagt
      661 caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg atgtcatccg
      721 tattctttca cgaaaaacaa aaaataatcc tgttctgata ggagaacccg gtgtagggaa
      781 aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc ctgaaaattt
      841 aaaagataaa acgatttttt cattagatat gggtgcattg atcgctggag caaaattccg
      901 tggtgaattt gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa gtgatggacg
      961 tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa cagaaggaag
     1021 tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac atttgatcgg
     1081 tgccacaact ttagatgagt accgtcaata tatggaaaaa gacaaagcgt tggaacgtcg
     1141 ctttcaaaag gtattagtca aagaaccaac agtggaagat acgatcagta ttctgcgtgg
     1201 attaaaagaa cgttttgaga tccaccatgg tgttaacatc catgacaacg cattagtagc
     1261 agcagctact ttatctgacc gttatattac tgatcggttc ttaccggaca aagcaatcga
     1321 tttgatcgat gaagccagtg caacgattcg ggtagaaatg aactctatgc caacggaatt
     1381 agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac tgaaaaaaga
     1441 atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag ctgaacttcg
     1501 tgaagaagca aatgcgatga agatgcagtg ggaaacagaa aaagaagaag tcaattctgt
     1561 ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg cagaaaataa
     1621 ctatgattta gaacgcgctg ctgtattgcg tcatggtacg atacctcaat tagaaaaaga
     1681 attgaaggaa ttagaagcta aagcaaaaga cagcgagatt aagatggttc aagagtccgt
     1741 taccgaaaat gagattgctc aagtcgttgg cagattaacg ggtatccccg taacgaaact
     1801 tgtagaaggt gaacgagaaa aactaattaa attgaatgaa acacttcata aacgagtgat
     1861 tggtcaagat gaagcagttg atgcggtcag tgacgcggta attcgttcac gggctggatt
     1921 gcaagaccca aatcgtcctc taggttcttt ccttttccta ggaccaactg gtgtcggaaa
     1981 aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc atatggtacg
     2041 gatcgatatg agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg gagctcctcc
     2101 aggctatgta ggttacgaag aaggtggtca attaacggaa gctgtcagac gaaatcctta
     2161 tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca atatcttgtt
     2221 acaagtccta gatgacggac gcttgactga ttcaaaaggg cgagtagtag atttcaaaaa
     2281 tacggttctg atcatgacca gcaatatcgg ttcacagtta ctgctggaag gcgtgactgc
     2341 tgatggaacg attccagaag cagtggctga acaagtcaac acgctattac gtggaaactt
     2401 caaaccagaa ttcttgaacc gaatcgacga cacgatttta tttacaccat tgagcctaga
     2461 caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc tggaacatca
     2521 agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa atgcttatga
     2581 accagcatat ggtgcaagac cattgaaacg atttatcact aaggaagtag aaacaccact
     2641 agctaaagaa atcgttgcag gacatgttat gcctaaatca aaagtaacga ttactttact
     2701 tgatggacaa cttcatttca aaacagaaga gctagaagaa atcgtctaat cttccattga
     2761 gactataaaa gaaaaaaggg gtctgacaca actagtgtca gacctccttt tttcaaataa
     2821 acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa gctgcccgct
     2881 tctgtcacaa cctcttttat taggtatttt tcactttttc taatttataa ctttcaaagt
     2941 aacgctcatc ttttttactt tgagaccatg caaacaatcc cattaaggca gcacctatcg
     3001 tatttgtcag caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca aacagtgtgc
     3061 catcagaagc agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat aagaattccc
     3121 agaactccca aaacacgccg caaagtccag caaacgcaaa gccgaataag aggaacaacc
     3181 aaggactggt ttttgatatc tcagcatctt tcatcaagta accaatcaat ccatagccaa
     3241 gagctgtcag gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa gagatgatac
     3301 tgatcaaatg catccctgtg cctaaaaaga cagagatgaa caagaaaaac cagtaaaaca
     3361 aaacgattgc tgctggtatt ttaagtttga aaactttcaa aatgacttgt ggcatgaaaa
     3421 tcaatgcaat ccctaataaa gattccacga taaatgtgat tccttgagaa gtcgaatatt
     3481 tttgtgtgat aaattcataa aaattatagc agacaacgaa tacaccaaaa gcaagtaaag
     3541 ctatttgcac ctttttgatt tctttaatca ctgaaagtcc ctccatctaa aaatcttttt
     3601 acttctgaaa gcaactcaat ttctgaagaa accacttttc cgttcaattt gatcaaacct
     3661 attgtatata agttcaggta gtgaaattga ttttcagctg tttcttgtag tgcagcgatt
     3721 tttttcgggt gggttccgcc ttgctgtcga gtatcagtat ataagccgat cactgggatg
     3781 cctttcgcat aagccacacc gatttccgat gctaccccgg catcgatcgt cagcccatct
     3841 agtaaagcaa tcattaaatc actagccaac actttttctg tgtcagcaag agcaatcatt
     3901 cgactgtctg cataagcagt tttgtcattg attgcttcat tttcttgagg aagatagatc
     3961 gaaagctcag aagagagctc tctgatttct ttaactaact gttggttata taacaaatca
     4021 cttttagcaa acattggtgc ggcaaaataa attttcatct aaacatcatc ctttttatca
     4081 aaaataatcg ctaaagtaag cttccatgtt tggaatgata cgctctagtg acttcagtgt
     4141 ttcttcatct gtttctaacc gttcgattct tgccaaataa gaagcacggc gataattcat
     4201 catcaagcaa gtcaatgttt ggatatccag tcgaacggat tttcctatag gttcatttaa
     4261 gacacggact tgtccctgtt catcccatgt taaagcgaag acaccattat tccaaggagc
     4321 gacaggatct tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg gaaaccgttg
     4381 aaggaattct tttacatcaa cgatccgagc cataaaatat ggttcgatct gctctttgat
     4441 ctgactatct tcaatcaaaa atgctaaagg ctcattttta taaatatctc catgtaccca
     4501 gtagatcatg gaaaaatgtg cggaaataaa gttccataag ccatttcgag cttcttgatc
     4561 aagatagaac atttctttta cgtgaaatat ttcttcagct acccagtaga acagaacacc
     4621 tcttggttca tggttgctat cgtagtagac agcggctgtg cgctcatctt cattttcgaa
     4681 acgccaatac tcttcccaat gaaaatcact gcggaataaa gcaccatggt tttgttgggc
     4741 aaatttgtca taaacggaaa atacgtctgg atgatcaact ggtaatcgct caaccatacc
     4801 acttactggg atttgtttag gtagctgtgt atctctgatt ttaaaggaaa gtttatcaga
     4861 catgatttcc catcctttac gccgatagta ggggatactg taaggataca aataagagat
     4921 ccattgctta ttcttgcgca tattatccaa tgcaacgata attagatctt gcatcaatcc
     4981 atggttagca tattctggat aagttccaac tccagtcaca ccacccattt tataacgtgc
     5041 accgtgaata ttgacttcac aaggatagat agcgatttga gagatcagtt tattttcgtg
     5101 gaaccatccg aacactttcg atacttccaa aatcggtttt ttggaacgga taaaagctcg
     5161 tttattctca aacccgcttt cctcgatgtc tgcttcagtc acttggaaca cataggaaag
     5221 caattcattg aattgatcga tatgttcttg agtgaccggt tttaattcaa gattttctct
     5281 aaaatctttt tgatccattt tttcatctcc atttattagc ttgataacag tatagcaaaa
     5341 ttctacaaaa aaaacttcta tagctgacta attaaaaact ctttaaatta ctgcttttcg
     5401 ttggtataat ggaaagtgct gattatatga aaagaggaga cgctatggat ataaagaaaa
     5461 tgaaagaacg ccaagagcag attcgtaatt tttcgatcat cgctcatatc gaccatggga
     5521 aatctacatt ggccgatcgt attttagaaa tgacgcacac tgtgacgtct cgcgaaatgc
     5581 aagatcagct gttagactcg atggatttgg aacgtgaacg tggaatcacg attaaattaa
     5641 atgctgtaga actcaattac acagcgaaaa atggagaaac atatatcttt cacttgattg
     5701 acaccccagg gcacgtcgac ttcacctatg aagtctctcg aagccttgct gcctgtgagg
     5761 gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc acaaacattg gccaatgttt
     5821 atttagcgtt agacaatgat ttggagatcc tgccagtcat caataaaatc gacctacccg
     5881 cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc gatgctagtg
     5941 aagctgtatt agccagtgcc aaatcaggga ttgggattga agatattctc gagcagatcg
     6001 tggaatatgt tcctgcacca agtggagact tagatgcacc gctaaaagct ttgatctttg
     6061 actctattta tgatagttac cgcggtgttg ttctaaatgt ccggatcacg gacggtatgg
     6121 tcaaacccgg agacaaaatc aaactgatga gtaatggcaa aacatttgat gttacagagg
     6181 tcggggtctt ctcgccaaaa gcagttgcta gagacttttt gatggtcggt gatgtcggtt
     6241 atatcacagc aagtatcaaa actgtccaag atacacgagt aggggataca gtaacgttag
     6301 cagacaatcc tgccgctgaa gctttaccag gttaccgaaa aatgaatccg atggtttatt
     6361 gtggtcttta tccaattgat acttctcgtt acaacgattt gcgggaagct ttagaaaaac
     6421 tgcaattgaa tgatgctgct ttacaattcg aaccagaaac ctctcaagca ttaggttttg
     6481 ggttccgctg tggattcctc ggacttcttc atatggatgt cgtccaagaa cgcttggaac
     6541 gggaattcaa tctagaattg atcacgactg caccatccgt tatttaccat gttaataaaa
     6601 cggatggtac gactgctaca gtagataacc cggcagattt tcctgaacct gtaacgattc
     6661 aggatgtaga agaaccattc gtcaaagcac agatcatggt cccaaatgat tttgtcggtg
     6721 cagtaatgga actttctcaa cgaaaacggg gagaattcat tacgatggat tatttagatg
     6781 attaccgagt aaatgtcgtc tataatattc cgctttctga aattgtcttt gactttttcg
     6841 acaaattgaa atcaagcacg aaaggctatg cctcattgga ttatgaaatg tctggttatc
     6901 aaaaaagcaa gttagtgaag atggacatct tgctgaatgg agaaaaagtc gatgcattaa
     6961 gctttatcgt tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt gaaaaactga
     7021 aaaaactaat cccacgccaa caatttgagg tgccaatcca agcagctatt ggacaaaaaa
     7081 tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa tgttatggtg
     7141 gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa agaagggaag aagcggatga
     7201 aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat ggccgttctg aaaatggatg
     7261 agcaagatag tgtgaaataa cttataatca atcgaaaaac ctattaaata aacctttttg
     7321 agtagatgca acactgagga ttatctgtat aaaagatttt aaactgtcat ttggttatca
     7381 cggcgatact aaaattaaca aaattggaat atttttcttg aagattttta gctgtcagta
     7441 tatgatgaaa gcatctcata ggaggtgctt ttttcatggt tgataattca agtggaaggg
     7501 ttcttaaatc tctaagaaaa gagaaaaaac tatctcaaaa aaagttagct gatttagcag
     7561 gtatttcaca aagtacttta gttaaatatg aaaaagggtc aagaaaaatc cctaaagacg
     7621 tagataatac tctaagcaag attctgaata tagaaactct aataaaagat gaggaagata
     7681 agattgagat tctcatcggt aaactaattg catacagaga tatgaataag ttacttaaca
     7741 aagagttagc tgataatata ggaattagtg aagtattact cagttatgtg ttaaatagaa
     7801 agagaaatcc gtcaaaagaa atgcaaaaaa aaatcgatat ttttctatta agtaatgaaa
     7861 aagaaatttt gaaggagatt aatcgtgata gtgagatatt tagtttatca aaagacgata
     7921 aaattgttat gggaaaaaga atacgagaag tccgaaagaa ccgggaagag acgcttgaaa
     7981 aatttggtaa aaactttacc atctatacag gcaaaaatgt tataagtaga tgggaaaaag
     8041 gtattaatat tccagatatt gaaaaactaa tgaatattgc ctatcttggt aaggtcactg
     8101 ttccttacct tatgtacgga gaagattata agaatatact tccaaaagat gaaagagtta
     8161 gcgattttaa aaagataaat tcttttagta tgggattaag gatgaggaaa attaggaaag
     8221 attattatct agaaagggag gagtttggaa agcttttttc tccttcaatt tcaaaatggt
     8281 ccatagatag gtatgagaat ggtagagata tacccaatac taatcgaatt atccaatatg
     8341 cttatatagg aaatctttct ttagaatttt tgatttacgg gatataaaaa tagtttacgt
     8401 cgatacttat taagttatcg acgtttttta ttatagaaaa aatttataaa aggaggtttc
     8461 ttgatatgat aagaaacaaa aagggtttgt tgaaagttgc tttactggca gcttttttct
     8521 aagtaacttt ttgacaagct caatggcaac acaagcggaa gaagtaatac aagaactaac
     8581 tgttatgaat aacagctaga taaaatgggc tatgtatgtt atccaacaat ttagtaatca
     8641 ctagaagcct agcgaaagtt cgcttgataa gttatagatg agtattgatt tgtagtgttt
     8701 ccaaatcaaa agggtacaga gccacatgaa atatgttgtc ctctgcagcg tcaggatggg
     8761 aatccatacc atggagattc ggagatctgt gagggaatac ttacgtggtt aatatgtcag
     8821 ttgtgttgga cgtagtaatg gtcatagtgg gtagattgac tgaattctta tatgagaaaa
     8881 tgaaaccaac aagaagatag ggttatttat acagaattgg ggatcttttc tggaaaagta
     8941 atcggataat gcgagattcg tacggtcttt tttaaatttc tctaaaaaaa acggcatcga
     9001 atgggttggg tgattgataa gcaatgaatc atagactgat atagaataaa aaatgattct
     9061 atttatcttt ttacatctat cgttttttca agtagccgca aattatatca gcattcaaca
     9121 atttttgtat acaagttcta gtaaaaggat ctgaagaaaa agttggttgt tgtgaagata
     9181 aaaaaagaat caggttttac tgagtttttc ttcaaaattt gtgttattag gttaggacaa
     9241 agataagaag aagtatgaag atttaaaaaa ggaatgagga aaagatagta atttccgatt
     9301 attggagttt ttttattttt cgtttgtcaa attaagttag acaaatcatc ttttgtgacg
     9361 taactcaaaa atacttccat tggtgtccga tagtgtaatg attttctagg aatatgattt
     9421 cgtttcgatg ccaccgattg gataaaggtt tcagatacac cattaaaatc catagcttta
     9481 tacaaaccat ctttacgtaa taagccattt gaatgttcat tcaatcctcg ttgagaaggt
     9541 gttcctggat ccgcaaaata aattgaaata tcgttggtat tactaatctt tttccaattt
     9601 gagaattctt ttccacaatc aaatgtgatg gatttaaata agttttttgg gacacaacga
     9661 aaccattgat tcagcctatt ttcaatgtct ttagcctgtc tgccttcggg tttcaaggta
     9721 atgatgactt tagatagccg ttcaactaat gtgatgaccg cgcttttatg ttttccaccc
     9781 acgatagtgt caccttccag gtgaccaaat tcatgatcaa actgggtata gtctgtcttt
     9841 ctgtctcgga ttgaccggcg aaacccttgt tttcctcttt tttctttatg accgtttggt
     9901 tttctttttc ccttcattgg taaatcgtga gtgtcaaagg ttttatcttt aaacattcga
     9961 tacagtgtgc gtaccgaaca agcaatagga aactctgctc gaccaataag gacatcagga
    10021 gcccaccctt gaacaacctt tttttgaatg tattcctgtt gctcatcagg taaaacaatc
    10081 ggatgtcttc cgcagttacg tttgtttttc ttatattgtt tatagtagct cattgcgctt
    10141 ccaccagatt taagaaaacg atagacctta taaatagttt gtcgagaacg ttgtaacaga
    10201 ttggctactt ttgaaacagg ttgtgattga ttgaaataag cttctatcat tacgagttca
    10261 gttggtgtaa gatggttata ggtcatttgt actcactcct tataattctt tcgtcggaac
    10321 tattttgagt gtagcacaaa tgactttttt tagttgtcta gcttaatttt acaatcggcg
    10381 atttataaag aagaagcgaa taagttgtga ttcctagatt tcgcgcgtga ttaattaatg
    10441 agcaaaaaat gcggaaagtt ttagtgttta atacacgagg acaaagtttc ttaggtattg
    10501 aaggcacacg tgtaacggtt gatgggtggt gtgattgttt cgaggttgga aaacatgttg
    10561 aacttatggc atttactgag ctaaaagata agaacaacgt ggaatttttc gagagtgata
    10621 ttggttggga cgatcatgaa gaagtttatg gaaaatttat tttgcggaag ggaaattctt
    10681 atacgaatgg ggaagcataa gtagcgattt gttagagatc attgatagga ttgaaattgt
    10741 cgggaatatt tatgagaatc tagagttatt tgaggtgata gaatgagtga tggttttgaa
    10801 caagtatcaa taattaaaaa tcaagttcgg gagattctta gaaaaaaagg ctatttggta
    10861 gatagttatt ttgaaggaga ttacgaaaca tgggtaggtg tctatgctcg tccagagaat
    10921 aaacctacct atttagatcc tacgacgtca gaagatgcct atttacaaaa taggtatcga
    10981 gtagatggtt ttaagcaaga ctttgcggag tggtttgaat gggaaattga aaacggagaa
    11041 gtaaaagaag aataagagta cttgttgtat caaaataagc cagtagaaca accaaaccag
    11101 tagctagtaa tgacaataat actgaggaat cggatcatta tggtatgatt gatatttctc
    11161 ttccagaaga tcttaagcca gatcctgtaa caattggcaa tagtgtcatg atttttgatt
    11221 cattagatga agctatgtca tatggttgga aagaaattaa ggatgaaggt agtaattggt
    11281 atggcatgag atttggtgat ggtgaagttg ccaaaaaagc tggtattcca attgggaaat
    11341 ggacagttga tttccacgaa gcaggttctt tttttgttct gtaattatag tgggagggta
    11401 aaatcattga tgaaatgtgt tcagataaga gcgccagata aaaatagaag agacagttca
    11461 cgtcgttatc acttttggta acggcgtttt gttttattat ataaaattct caggaaaagg
    11521 aggaattctt gtgtatgcaa aaacgaaaat gaattggttg aaagcactcg cattggtggc
    11581 tttttctcaa ttctgtttct tggtgggaac tgctcaagca catgcagaag aaacgacggc
    11641 tatacaacct acgatggaag ccattgccca agtcgcttct gaagtgacag aagtccaacc
    11701 agcagaagcc ggggtcccag ctggtgaagc cactgttgaa caggcccctg tgacagaagc
    11761 gccaacggtc aattccaaaa cgactctcga tcagacgatt ggggaaacac aacacccagg
    11821 gaatagtcct ggtacttcac aaccagttga agaaactgcc tcaattacaa cccctgcttc
    11881 agaaacgtcc actctagaag gagaaaaagc tgccgaagca acgccttctg tgcagccaga
    11941 gtcttctgag aaacaagcgg aagaaaagaa acgaatagaa gaaacagtgg aacaaccagc
    12001 ggctactgct gacaaagaag gaaaaaatga ggaaaacgtt gaaaaaacaa tctcttcaac
    12061 cgcagctagt caaaatcaac caacgaaacc aaacaaagaa tctacggcta gtattgaaaa
    12121 tcaaaagaaa aaagagcaga acattccaaa aacaggtaca cctacactgt tagtaacagt
    12181 accaaccaat cgttcggttc gtgttcgccg tgcaaccaat gcagctgttt taggtggtcg
    12241 gaaaattgat tggggcgaaa atattggtgt tacttctagt ccgaacaatc taaacttcac
    12301 tttaaattat atggttcgtt ttgagattaa tggacaagtg gcgttttgta ttgagcctgg
    12361 tgtctcagcc gcagaaggtc aagtttatac agctactgct ttgaaaaact atttgaaaaa
    12421 ttcaaatgta cggaaaaaaa caagcttgat ctcttattta ggatatgtca caaataaaga
    12481 caaatccgat gaacaataca ttgcggcaca atttatgatt tgggaagctt tgggcacaaa
    12541 aatcccaatc aaaaatatcc gtgttgatta tgatcgtcga aaagcagaaa ttcaacgttt
    12601 gattaataac tttggtgaaa aagcaacttt taataatcaa acacatacgg taaaaattgg
    12661 cgaaacgttg aaagtaaaga ataaaaatga ttttttgaaa tatgtgaaag acattcaaaa
    12721 accaaaaggt gtcaatgtat ctattcacgg caatgaagta tggattacag tcacaaaaga
    12781 ggcaccggat aaagcaaaaa ttcaattgaa taaaattaaa gttcaaggtg cgccattagc
    12841 ctatcgtcgt ccaggagctc aaacaattgg tgtgttacat ccaacagaac ctaatgcgtc
    12901 tgttattaat ttacaaattc taaaacaagg aaacattcgt gtgtttaaag aagatgcgga
    12961 aactggtgtg aaagcacaag gtgcagcttc tttagaaaat gcggtctatg gtttgtatca
    13021 agcagatggg aaaaagatta aagaaattac cttgaaaaaa attaacggca aagtacaggc
    13081 agaaattaaa gatcttgatc ctggcaaata tgttttaaaa gaaatcaaag cgccaaaagg
    13141 ttatgtgctt tctgatgaag caattgaaat tgttgttgaa cctggaaaaa ctgtcaatgc
    13201 gaccgcaaaa gatcaagtga tcaaaggaac cattgattta gtgaaagttg ctaataaaga
    13261 tttagtcgat tcaacgaatc cagataacaa accaaaatta gctggcattg aaatcacttt
    13321 gacgtcaaaa acaactggca aagtggtgaa aaccgttgtg acagacaatg atggttatgc
    13381 tacttttggt aaaaataccg ttgtctttga tacgtacatt ctttctgaaa ccaaaggaaa
    13441 agaagggtat aaactatttg aaccacttga agtcacaatt tctgaaaatg ggcaaacttt
    13501 ccattatgtt ttagaagata aagtaattga acaacgtctt aaagtggtga aagtggatca
    13561 agaaacagga aaagtgattc cacttgctaa tacagaattc aagatttttg atactttagc
    13621 caatcgttat gtcacaatgg agattccaaa tgatacagaa acaacggata tctttaaaac
    13681 caatgaaaaa ggctttttcc taactaacgg tacacttact tatggaaaag atcgctatcg
    13741 tttggaagaa atcaaagccc ccgtaaatta cgttttgaat caaaacccat tagtgttctc
    13801 gattaccaat gatacagaac cgatcaaagt gatccatttt gcgaaccgtc aagcacggaa
    13861 aagtgtaaaa ttattcaagt atgaagaatg gaacaagaag aaaacgccgc tcgcaggtgt
    13921 gaactttacc ttgtataacg gtaaaaatca agcaattggt gaatatacca ctgatcaaaa
    13981 cggggaaatt ttagtgaaag gtctagttgc cgatgactat tacttcttgg aaaatgcccc
    14041 attagaaagt ttccaaccaa atacagataa acaagcattc acggttgcat tcaaacaaaa
    14101 tgcgccagat gatggggaaa cgttagtggt ttctgtacgt aattacttga ttccaccaaa
    14161 actagaaaca caagcgtcaa ataaagccga tggtgaaaaa gtggttgatc cattagaaac
    14221 gatcgtcatt aaagacagtg tttcttatac caacttgttt gtcggaaaag aatacaccat
    14281 ttcaggagta ttgatgaaca aggcaacgaa tgcgccaatt ttacaagatg gcaaagaaat
    14341 cacaagtacc ttggccttta ttgcagacaa agtcaatggc acaaaagacc ttgaatttgt
    14401 ggttaatgct agtgttctga aaggtcaaac gatcgttgtt tttgaaaaac ttttccgtga
    14461 tggcattgaa gttgcagcac atgcggatat caacgatgaa gcccaaagtg tgaaagtgaa
    14521 taatccaacc gttcatacca cggctgtctt tgaagacgga ttgacagtcg cagacccaac
    14581 aggattgttt accttaaacg ataccgttga aattaccgag attcttccag gaaaagcata
    14641 cttggtcaaa gggatcttaa tggataaagc aaccggtgaa cccttgcttg tgcatggaaa
    14701 tacggtagaa acgagtgttg gctttattgc caaagcagaa aatgaaacgg tgatcgtacc
    14761 atttaccttt gatttgacag gtataaatgg tcgtgaaatc gtcgtgtttg aaagtctttt
    14821 tagaaatgat gaagaactag caaaccataa agacatcaac gacaaagggc aaacggtacg
    14881 agtaactaac ccaagtgtac gtacaacggc tacacataaa cccactggct tgaaagaggt
    14941 taatccatta acgaaagtca ccattacaga cgctgtgaaa taccacgatt tggtcgttgg
    15001 aaaaacatac acagtcaacg gtgttctaat ggacaaagta acggaaaaac cagtgctagt
    15061 agatggaaaa gaagtcacaa gtaccttaac gtttgtggca gaaacaacgg atggtacagt
    15121 caatctagac tttattctag acgcacgtgc gttgcgaggc aaagaagtcg tggtctttga
    15181 agatctgttt agagaaggcg tattattggc ttctcacgca gatattaccg acaatgacca
    15241 aaccatcaaa atcagtaatc caaccattac gacgaaagcg acgaataaaa atggcggaaa
    15301 agagatcttg gccttgccaa atcaaactgt gcttgaatgg gtcaaagtag acggtcttgc
    15361 gattggtcaa atctatcgct taattgttca aggctactta acaccagacg gcacaccatt
    15421 tgaaggcact catgcggaaa aagtctttgc ggccgataaa gaaatgatgg aattcttatt
    15481 tgaattctta gtggacggtc gcaatcttgc aggaaaaggc ttgagttttg cggaatggct
    15541 ccaagcgaaa acggaagatg agaaagaaac gtttgaagaa gtggcgaaac acaatgtaga
    15601 tctaacaaac aaagatcaaa cggttcaaat tgtggaagcg ccaaaaccac cgcaaccagt
    15661 gaaaccagaa caacgggtta aaccagctac tgttgtacca atgttgccaa aaacaggtga
    15721 acaaacaagt agtatactta tggttcttgg attagtagta ttagtcattg ctttaggtat
    15781 aacactttat aaagtacgta aagccaaaga ttagtcatga ggaacaagcg gttaggattt
    15841 ttaaccgctt gtttgattgt aatgtagaaa tatttagaat ttattttggt ttttaagagg
    15901 aggaaaaact ttatggcaga aaaagcattg cgtatgaaga aagagttttt aggtcaagta
    15961 tttttattgg gttcacggga acctaatcca ccaaaggaaa ttaatggtgt gtctgggcga
    16021 tatgttcaat tacaagcaga gtgttcgcga gaagaacagc ctgctttttt tgtttatctc
    16081 acagctgaag aggataaaag attacgatta ccagaaaaag aggtgcgtat ttcatttgaa
    16141 gaagcttatt ttgtttttcg taaagaacga gtgaatagtc gtgaaacaaa tgatatggga
    16201 caaacagagc gtcgaacaac tggctatcaa gatgttggtg tctttttaac tggtgtaaaa
    16261 attggggagg ttttgtaaga tgggattaaa atttccaaat aattatgaca ttacagaagc
    16321 attagatgaa acttgtttag gagccgtacg ctttggcgca ttagaagagt ctgatcgtgt
    16381 atttgataat gaaacaggcg attatacggg agaaattcga tcacagcgag cacaattagt
    16441 ttttggaaat ccagatgaac caaagattgc acgacgagtt aaatttccag cagatgtaga
    16501 tttgactggt tttaaacgac gtgatcctgt gaaattaatt gatccagaat atcatgtggt
    16561 atatattcca gcacaagatg gcaatcgtga atattcagat ttagtgattt atgcgaaagg
    16621 attagagaaa gtagaagctc caacaaataa tcctaatatg aaacaatcac aagaaaaagg
    16681 tttagatcaa aaacaaccaa gtgttgattc taaggaaaaa gagaaaaaat aaggtttttt
    16741 tgaaagaata ggaaggagtc ttcctattct ttttatctaa gatagaagga aggggagatt
    16801 ttgaattcag ataaaaccaa tcaaatactt tgagagattg tgcttgttat caaaatatcc
    16861 aacaattgat tgagtatttt ggagacaatc caacgatttt tgtacaaaca aatgcatata
    16921 tttgtgcggt tattgggcag tgtatgtttt tacgtgatct tgttcgtttt tcattaccgt
    16981 accagtggaa acaagatcat caggaagtga tttggattcg tatagtacga atatgtaata
    17041 tgtgtgcaca cccttataaa aatcgattaa atctttttcg attatgtttt tttactaaat
    17101 gtgatattcc tgtattagtt gctttttgtc aaaagttttt agcacaggaa aagtaaaaac
    17161 agaaaggagg aatgtgacaa ttgaaagctc attttttcaa gtatcgtggt cggcgaattc
    17221 gctatttcaa tcgttattta tggttttgct attatggttt ctttttgttt ccatttttga
    17281 ttctgttagg ttggtttagt tggaaagtat tttatccacg tttaagtacg ttttcgaatt
    17341 ggcaaatgta tcttattcct ggaataagta ttgtgttgta tttgttgctg gttagtgggt
    17401 taattttagg gttgatgcat tggtcgcgac taaaagaagg atattttgcc tctgtttatt
    17461 ggcgtcagtt acttgttcga atgttaattg acaatcggtt tgtttataca aagaaacaag
    17521 ttagtgagaa acaaacaagg gaaaaaatga gattaccaaa agtttatttt tatcaaaaga
    17581 aacaagaatt agtggtttct tttcctttag atggtgggcg ttttcacgaa cgatttttaa
    17641 aaatgggtca tttgttatct gaagtatttt tgcgtgattt aattcgtgaa gaacgagaaa
    17701 aagcccgaat caattatgta ttcctttcag attctggtcg gattcctatt gatcaatgta
    17761 tagcagaaaa tggacgtatt catttaatga aaactttgga ttgggtatat gatcaatcac
    17821 caaatatgtt gattgctggt gcaatcggtg gtgggaaaac ctatttattg tattctttaa
    17881 ttcaagcttg tttaagtgtt gggacagtag atatttgcga tggaaaagcg gcagatttag
    17941 cagcattagg tgacgtcgat gtattcaaag gacatgtgtt ttacaaaata aatgaagaaa
    18001 tgattatttg tttacgaaat gcattaaaag aaatgaatca acggtatgcc tatatgaaaa
    18061 ccatgaaaga tcctaaatat gaaccaggga aaaattatgc ttattatggg ttaccaccgc
    18121 attttatctt ttttgatgaa tgggcagctt tttatggttc gttggatatg gaagcgagaa
    18181 atagagtcga taaattgatt cagccattag tattaaaggg tcgtcaagca gggatgtaca
    18241 ttattttagc aatgcaacga ccagatgcag aatattttcc aagtggtgtt cgggataatt
    18301 taacattccg tatttctgtt ggtcgattgt ctcccattgg ttatttaatg acttttggag
    18361 aagattttaa aaataagcca ttttacaaca aagatcaacc tggtcgtgga tatgcttttc
    18421 caggatttga tgtacctgta gagttttttg cgccatttgt ttacaaagat tatcacttta
    18481 tcaaagaatt tgagaaattt actgaaatga ttgtctatga ttttacagta aaagaggata
    18541 gtcaagatca aacgatggga gatgataatc agttgttaca agaacaacta gagcgccaaa
    18601 tgtatttagc aaaagatcca aaaggaacaa gtttttctga tgcgcatatt ttgtaatcaa
    18661 ctaaggaggt gaagttaaat ggcagagagt tgttattata gagtagctac agcaatacga
    18721 atgattaatc cttctctttc ttccagaacg ttttatgatt ggttgaatcg tattgaacaa
    18781 gtaactgatt accgcttttt gcgcaaagaa cgtgttttta caggaaaagt tattaatcaa
    18841 gtattgctaa cgaaaaaaga tatagaacga ttgactcgat tgtatcatta tcgagtagat
    18901 ttagaagagg atttaacttt gtcaatttat cgcgtttttt ctcctgaaaa atatagtgaa
    18961 ataacaaaat tagatcattt aattttgtag ttattatcta tcgggagggc tctagacgta
    19021 gtctggtagc cgcactctgc ctggcggtgc gcattaaggc tgcgaagcat gggcctataa
    19081 tgcgcactcg ctagcagcgt gtgcagctca caaagaggtc gtgcgcttgc gcacggcctc
    19141 tttgtgtaaa gccccccgat atataatatg gggggcagaa ataacataac acatagaaaa
    19201 aagttttata aacccttttg tatcaagtga ttatctaagg tcagcagttg tactactttt
    19261 cccaaaaaac gagggggact agttgatata tcggtgtttc taaagggtta aaaaaaatcg
    19321 attttgttat tttataaaag gagcgagaaa atgacaccga ttgagctaaa aagctacaga
    19381 aagaagttaa gtgcaattca taatataagt ggagataaaa agccgctatc acaagcaaga
    19441 tttgcgcaat tgatgggctg ctcaaaagat tacattagaa agatggaaca agggaaaaga
    19501 aaaatcccag aggattttga acaacgaatc aaggaacgct ttcgattgta tatgaaaagc
    19561 ggaatagatc ttcgagcaat gattgattat ttgcgtcttt ccttttttgg gacagactac
    19621 aaaacagtga ttcaacgtgt attacgaatg gatctgacag aatttgaaac acgtccaacg
    19681 ggcttgtatc tttttgactg tctttctgtt cgtgggaata tctgggtgtt ctggcattca
    19741 aaacgaactg taacagaaaa tgtacttgtg caattttcag gacaaggttg ccgggaatat
    19801 gaggaagtca tgcgtgaaca acaaacagat tggcgaacat ggctatattc catttggaaa
    19861 aatcaaggaa agttggaagc tggttataca cgtgtgcaat gtagccgatt cgatattgcg
    19921 ttagatgaac tttggggatt ggaaacggaa gaacatttcg atctctttga attactagaa
    19981 aaacaaaaag caggtttatt agaaatggac tttaagagat tccagaatat tggcggttgt
    20041 tttttagatg atggctattt tcgaagtgaa ggattatctt tgtattttgg atcgcgaaag
    20101 agtcctctct tttttaattt ttatgagaaa cgttttgaaa ttgcgaaccg agaaaaaatt
    20161 tcagaattag aagcattaac gaaatatggt atttataatc gatatgagtt acggttagcc
    20221 aatgagaaag caacacaagc agtagaagca tttattttag gtgattcacc gaaacgttta
    20281 ggagagattg gcgttggttt aattaatgca aaacttattg tttgggatag actggaaagt
    20341 gatgcacgga ttgttgataa acgatgggca aaattatttc gttcgatgga agaattggat
    20401 tttacagtaa agccagaaaa attctcatta gatcgttcag aacgctggtt tttgaaacaa
    20461 gtagctccga cattaaagtt gttacaagaa gcagatcgta ttttaggaag agactttgta
    20521 caagaaggga ttgataatgc cgaactaaat gacgagcaac aaaactatct agaatggatg
    20581 ggaacgtcag gagggaaaat tgcatgaatg catatacgac aacagaatgg ctgcgcctgt
    20641 tggatttaaa ggctgcaatc gaagcattaa atgaaaagat ggttgatttg tcttattttc
    20701 gttttcgagt tccttatatt gaacaagcgg taaaagctgg acgttatcag gaaaaagaga
    20761 actggcaaga aattgcacgc ttattagaag ttcgtaaggg atacgaacaa gaactcgaag
    20821 agttggaatt tagccgaagg aaaggaaggc tagaattcat tcgcttttat cgattttcat
    20881 tgccaatacc tgcgatttta gcggttaaaa aaggctgcga taaaatgaag atatatgaaa
    20941 actgtgtggc agcacttagc agcgaaaaac ctcttatgga agaaatttca ttggtgactg
    21001 tcacttggga aatgagtcgc cagccaacag aagaacagac atatctttct ttagaagaga
    21061 ttgagataga attagaggaa attggtcgct atgccacttg ttctacctat tgtggatcgg
    21121 tcattagtat tgctggtgtg attgtctaaa tgaaaaaaat tatgatttat gttggtgcgt
    21181 attggtcacg tgatccaaca gtattagaaa atccagaggc ggtacactat tgcttgcgcc
    21241 aattattcta tctgtataaa gaacgattag aatcgttgat tcgtcagtta ccatatactg
    21301 acagacgttt agatgaactt cttctaaggt atccagcaat gtataaacga cgaaaaaata
    21361 gattgttacc agaagagtat cccattgaaa aacatgaact tgaagggcga tttgtggctt
    21421 atttctatga tgatgttcgt atgcgcttag tagaacaacg aatggaaata gaaaatgatc
    21481 gctactattt tatcaattat tgtaagaagc gtaagtatca agtaactgat gatttttatg
    21541 attgtatttt acaagatggg gaagtgattc tatccaaaat tgctccaagt tttcgtgaac
    21601 ttgtgccgat tgattttttg aaaaaatgtc atttgcggat tcttccgtaa aaaagagcta
    21661 taaaaggaaa gaaaagtaga agccaaatcg gtttctgctt ttttattttt tttaggaagg
    21721 agaagatggt atgtcactgg aatttttagg agagccaatt gaaaaggaag agttcgtttc
    21781 gcattatatg gatttgttta ttcaggtgat aaaaaacttt gtgcgattga tgaattttat
    21841 ccacgcgaac aagactatct agccgctgaa cgaataaaaa tcagtttatt atgggaaaaa
    21901 ttcacagatg aaaataaccg accgccagat tatcgctatt tatcagattg tttaacgaat
    21961 aatgtgcttt ctaaagaata cctatttcac aaacgtgaga aaaatcaaat gtcggttgaa
    22021 catttttgtg attgttatat tgcattattg cgtagtcaac agttattaac agtagaacga
    22081 tatggtgcag agtttggtta tgtcatgcaa gctgagcgtc atagagctac aagatgctat
    22141 gaaaaagaag atcaagtgat tgatggctat gaagaattat gtattcgaaa caatcgctat
    22201 ttgcaacaac gtttatatga acaattgatg atggattttt caaatctcta tagagggtat
    22261 gaatgttcga tgattcaaga aaggtagaag aaaaggggga atttttatgt ggcgattatt
    22321 atttttggtg gtatgtggta tggcattgtt ctatgtgtat cgtgtctatc tctatccacg
    22381 aatggaacga tggacaagtt ggtatagaga tcctttaaaa caacgaaaag gattgaagcg
    22441 gtatcgttat gaattacttg ttgaattttc ggatgggcga caagcacagt attatttttt
    22501 tgcaaactat ccaaaataca acaccagtga actattgatt caattattca aaaataatcc
    22561 atatgttggg ttagaagaaa acggacggga gttcttttat cgttcagata atattagtgc
    22621 ggcgtctatg aacaaaacac gaattgcata aaaaattgga ggaataatca tgaaaaaaac
    22681 aacattagca gtcgtaacaa cactaggaat ttcattattt tcgtttcaat tagggagttc
    22741 attggtttat gcagatgaaa ctgtagaaac acctaagtca gaaattgtag aaacattgcc
    22801 aggggaagga ttaccagatg ttgagacaga gctaaatgga ggaacaacgg aaaaaccagc
    22861 agaacctgaa caattgcagt cagagaaacc tgaaaaagag ccagtgacac cagaaccaga
    22921 gtttcctgaa tcaactttac cgactgattc gatcgaagaa gaaggtgtgc atagttcaac
    22981 aacaacggat tcaagtatag tcacaactcc ggaacaatca ggaacaacca gttcttctga
    23041 aaagaatact gattcgtcaa gtacggaaca gccaacgatg tcgtctacca gtgaagaaca
    23101 aaaagaagaa ccgaaacaac ctgttgaaaa atcagaagca attccaaaag aaaataaacc
    23161 tattgttcca gcaaaagaag tcacagtatc ggtaacccca agcggtgaaa ttacgacgaa
    23221 tacttctcaa ggaatgagtg taccgattgt cactagtaat gtagaggaat tgacccatat
    23281 tccaacacca accaccccat taaaagtagc aggtgatcaa acaattgttg gtgtaaaaga
    23341 tggtattcca ttaatccaag atagtgaagg gaatttggta aaggatttgt cgattcctgt
    23401 aaagaaactt ccaagtggca atattgaaat caaaacagca gatggaaaaa caaaagtctt
    23461 accaaagact ggtgaagaaa tacatgttgc actttctgtt ctaggaggag tgttgacaag
    23521 tattgctgga tttgttggat ataaaagaaa gagaaaagga atagaaaatg agggtaattg
    23581 aatggactat ttttcagaaa aacaagctta tcgttgtcaa tcgtacgtac tagtaaaaac
    23641 atactagaag gagtagctta tgaaaaaacg acaactacgg cggaaagaaa aagtttatac
    23701 agagaaagta ccgaagcaaa agaaaatcca ttcaaaaaaa gcagtgaaat ggatttggtg
    23761 tttggtttta tttctttgtt tttcaggggg cttggcattt tttcaatcca tgaaaacgaa
    23821 ggttcaagtg tcgtttttga aagatgaaat ccaacaatta caaaagaaag aagtacaaag
    23881 tcggtcagaa ttaacgtaca cacctgaaat tgaatccttt atgaatcgtt ttgttgcttt
    23941 gtatatgaat acattcgata atcatgaaca agaacaacaa cgtcaagaaa cattaaaaaa
    24001 agagttttat gcacaaaact ttattcaaga accaggaagt gtttcaggtg ttcgaaaatt
    24061 aactgctgct tcgtttattg gattaaaaga aattgaaacc attccaactg ctagttataa
    24121 agtaacttat gatattcgct cgatgaatcc agagagtaag aaagaaacaa gtgtgacaat
    24181 cacacaagta ctcaatcttc ccttttctat ttcaaacaag ggctgtcgta ttattgctta
    24241 tccttatttt acggcactac caaagaatac agaaaaacaa cgaatgatta cttatgatga
    24301 cacattatac gaaacggttg atgcgtcgga agcgaaagat gttgaaggat ttgtggaaga
    24361 tttcttagat aagtatgcga aaaacaatgc aaaagatatg tcatacatga tgaaagaacc
    24421 tgaaggactc aatcaaacag ctaaagttga tcaagtagag atgaaagtat tgcgttataa
    24481 agaaacgaaa aaacctttta ttgtaaaagc aacggtcgtc tttcgagatg gtgatacgtc
    24541 acttttacaa aaagaacaaa tgacgattgt cattacgaaa aaaacaaatc agtattatgt
    24601 tgaaaaatta acacataatt ggttggatag gaaaggagaa gaacggaaat gacactgaag
    24661 ggaattgtag attttatatt aacgcaaggt ggctattttt taataatggt ggttgtttat
    24721 ctgggattgc gaaattttat gaaatcaaaa gtaggaaatg cattaatatc tatcgcagca
    24781 ggtgccgcag tttatttctt cttaaatgat ccaacacgtg tatttgatgc gtttggtaaa
    24841 atcattgaaa aattatttat ggattaggag gatagtgaag tggattggga accttatgat
    24901 tattcgattg tttatgcgga accttatcgc gtacaacaga ttacaagcaa ttggcgtctt
    24961 ccatttccag ttgcattagt caatgcagca acttttttgt tggtcgtaat ctttgaatat
    25021 ttatttttac gttcatttat tggttgggtc atttcttttt ttccaccagc aggagttgtt
    25081 ttttatatag gactccccta tttatgtgtg ttagcactgg caaaactaaa acctgatgga
    25141 aaaaaagtgc atttttattt atgggattgg ttttgttata tgattcgtat gatgaatggt
    25201 gattgtgtgt attataactg ggacaaggta tcaaacaatg cattggaaga attacttttt
    25261 gtgaataagt atgtttattt agaggattag aaaaagggac aatcaactgg ttgttccttt
    25321 tttattggga ggtgaaattc attggtgctt gtaaaagaac cagtaaaagc attcaagaaa
    25381 aattgtttgt taaatggaga ggattgtatc tgggcctact atcatgtttt tccacgaaca
    25441 atttcaaaaa acacaccaaa aaagacacag gaaataaaag aaatgatggc tcgtttactg
    25501 ttgcttttag cagattatga agatgtggag ttggtgatgt atccacgaac aatgaattta
    25561 cggacgaagt ttgacacatt aggaaaggga ttttctcccg atagtcaagc aattggtgcg
    25621 tattatgcaa aagaaacagt gacattgttg gaacgagaag gtcaagtcta tcgtccttca
    25681 tttattttag gaatcaaagt caaagaaaaa gcaacggtgg agagttttca tgatgtaata
    25741 aatctaatct ggttagattt agcaggaagt ttagcacgct tatttggatt agaagtagat
    25801 gatttggaaa ctttgttttc tgaaagtgat ccttatgtaa aagaagtgac acaattactt
    25861 tatagctttt ctattcgtcc cttagtaaaa aaagagctgt tgtatctcaa tcgtctgcaa
    25921 tatattcgag gaatggctca caaagtagaa gaacaaaata attgggaagt actgaaagac
    25981 aatcaaggaa cgtatgtacg tataccgaag gaacataaag gccttcttca attagaagat
    26041 gagttaggga gtagttattt agcattgttg cctattgggg aaacacctga aaatatgaga
    26101 aattgtgatc tatttcaatt tgctcaggat ctttctttcc cagcagaatt gcgaatgaaa
    26161 attcactatc cacgcttgaa aggacaggga ggtattggtt taaaactgaa ttggctatcg
    26221 gaacggttca aaacagagca taaggaattg gttcaaaatg atgaagaacc tagtgaacgc
    26281 ttattaactg ttcgaggatt agtaaaacat ttaaagcaac gagtagataa tgggcaacct
    26341 gtcgtcgatt ggatcgcatg ttttattgta tacggtcaaa cggttgaaga agttcgtcaa
    26401 aatagtgcaa cggttattca acaacttcat acgattatcc atatccaccg tgcaaaaaat
    26461 gatcaacgag aattgttcta taaatgttta ttagggcaac cattgggacg gcagttacat
    26521 tggaagcacc gaacaactgt agaagcagtt ggcgaatggt tgttttcagc agtatcagaa
    26581 ttaggtatgg aatccggctg gtatattggt cggcaagata cattagggga tggtggttct
    26641 cgaggagaaa aaacaccatt agaagaatta atttatgcgt caaatcgttt tgtctttttg
    26701 aatcttctag ccattgcgga aggaatcaaa ggtgcgttgt ttgatgcgcc tcatgtagca
    26761 gtaactggaa aaaccggtaa agggaagacc tttttagttg gcttgctctt tttgtatagt
    26821 tcgtttatga atgtacaaag tttatttgtg gatcccaaag gagaaaagcg caaatggttt
    26881 acgaaactca ttcaagaccc gtattatcag aaacattacc ctttgtttgt agaacatttg
    26941 caaaaaatga gttatgtcac gttagatgct acaaatcccc gtaattatgg tgtattagat
    27001 ccaattgttt gtttatcccg agtagatgcg aaacaagtag cccaggatat gattgatgag
    27061 ttaagtccgt taggtaccaa tcacctttta aaaggggcgg tattaaaggg aatccaagaa
    27121 gttttagttc aaaaagaagc cggacaacaa gttggcttga tgcatgtggt cgactattta
    27181 gaacaaatgg aacaaaaaga ggttcgagaa tatggtgagt ttttacgctt gacggtagaa
    27241 gattcgattt tacgtttagg attttcttat ggagaaaacc cgggattaga ctttaatgcc
    27301 aaaacaacga ttttagagat ccaagatttg aaattaccaa atgacaatgt ccgaccagaa
    27361 ctatatacag atgcggatcg aaagtctttg tgtttgatga ttagtttagg tcgtttttgt
    27421 gaaatgtttg gaaaacgaga tagtagtaag aaaacagcga tttattttac agaagcatgg
    27481 gtatttaata attcgaacgc aggacgtagt attattgcag caatggcacg tgtggggcgt
    27541 tcccagatga atcaattagt gttagataca caatttattg gtgacttagg ttcagataaa
    27601 gaaaaaggga attttggtgt cgtctttgct tttgatgaag acagtgagcg agaaaagatt
    27661 ttgaatcatt tacgtttgga agtaaatgaa gcgaacttag ctgaaatgag ccacatgatt
    27721 aaaggacagt gttggatgtt agatccctat ggacgtgtag gaaaactcaa tgtacacagt
    27781 ttgtttgaag aaatgaccgc tgcattacaa acaacggaaa aaacaagtca ctctcaagta
    27841 gaagaacagg atacgttaat catgcaatag aaatgaggtg aaacaatggc ttatataaag
    27901 cataaggaaa agaaatggtg cgttttgttt tttttaacga ttggattcgg actgcttttt
    27961 gttggtggat tggtgtatgc ggcagaaaag caggtggatc aatcgatcta tgagattggg
    28021 aaatttcata ttgatacctt ttcttcttat ttatctgata gtgaattttc attaggcaaa
    28081 atgttatttg gggcaaatgg cacagatttt ctaaaaatga tgacgaatat gttctttagt
    28141 ttaagtaaat tgatttggca aggccttgat tttgtcattg agaagctcta tgaaggagct
    28201 gcaatgaacg agttaattca ccagtttttc actttttcgg atactattta ttcaaaaata
    28261 tacgatccta ttggtgcggg gattgtcgtc ttgtatgtgt tatatctatt tactcttcgt
    28321 atatttagag ggaaacatta cgcaaaaatg gtgtttattc gatttctctt cgttgtcatt
    28381 ttttcaatgg tttggtttgg ctatggtagt aacgtaccta gtaaaggaga agcttttacc
    28441 aaagggttaa atgatttgtc cgtggaggta gaaggcttta ttttttctgc tacaaatagt
    28501 ctagatgaat tggcgacaaa agatgtcaat ggtgttttag tgccaacgac acaaaaggaa
    28561 gccattcaaa aagtgcgcca actttattat aaagcggctg ttgttgatcc gtatatattg
    28621 ttaaattatg ggacaacaaa tttagaagaa ttgtatcaag cagagattga accacaagaa
    28681 tttctagcca aggaagcgtc aagtgaagaa ttatctgata ttgagacaaa agtgaccact
    28741 gctgcaggag aagatagagg gaaaaagaag gcttatcgtg tctatattca accaactaaa
    28801 gctgtttata aagcagtcgt tggttctatg gtgccattgc ttaatctaag tattggatta
    28861 ccacttgtag tgattggcat gattcgtttt atctttcaaa tgttggtgtt gtgtttatta
    28921 cttgtgattc ctttttcatt actcgcttct tttgttccag tatttgatta tatgctcatg
    28981 aataccttaa agtccttttt gggcttgatg tttcaaaaat ctttttacag tgtgattatt
    29041 ttggtggcat ttttgatcta taacatagta gacgatttaa ttccaatgaa tactgttttt
    29101 ggttttttag gaaatatgtt tgtcaaagca ctcctatcaa ttttactcat tgtaaaacgg
    29161 aaagatttat taaagaaact agggttagga gctgcagatc aaactataaa caacacgaaa
    29221 aacatagcaa gtcattcttt gtctcaaatg aaacaactgc ctggtcaact atcaactgct
    29281 ggacaaaatt tggcaatcaa aggtgcagga ttagcaggga atgtgtatcg agcaacgaat
    29341 ccactcgctt ctgcagcgat acatggattt caaaagtttc aacaaaaacg tacaccacaa
    29401 gaatcggtaa aagggaggca agtggttaat ccctttgaac taggtgggcg ttcaaatcaa
    29461 gccttgcctt atagtggtcg tcatgttcgt tatcatgggg tagagcctga aatgtcttct
    29521 accaaatcga aaacagagtg gaattctggt ctttcaaatg gtttgattcc aaattcgtcc
    29581 atgaattcag taagaccaaa acaacaggta caatcattgg cacaattgag taagcaacaa
    29641 gggaataatc aaagaaaacc attgttaaat aaaggtatcg cttttgttgg tcaatcgatt
    29701 ccgttgcctt ctggaaaagt acccgtgcca caattaacag ctgctggttc tctttcacaa
    29761 cagattcgcc aagtgaaaaa tcaggcgaaa aatgggagtt atccagcaat gcagcagtta
    29821 ttgaaccaat actctgttcc taattcgtcg gtagccactc tatcaggaaa tcgttctgtg
    29881 caacaaccgg ggcaacgttc gtctgaaagt aaaccgtcga tttccgcacc aaaatcgtta
    29941 caacctgcat tttcttcttt agataagcca acagagcaat cggaaaaaat agaaaaggat
    30001 cctaaaacac tgccgatttt ggacgcaaaa ccaggaactg attttacttt accaaaagga
    30061 cgaacagtcc aaaaaccatt atcgaagaaa ggatgaggaa gaaatgaaga aagggtgtct
    30121 aggttgtctt cttttcttta ttcttccctt ttttctattg ccattctttt tttcaggaga
    30181 actttctgag catggaggag aagctacagg tctagctgtc gcttttaatg gggaatatac
    30241 cgaaggatta ccgatttttt cagagattaa aggacgtggt ccaatcacag atgaacatgc
    30301 gcaagcagca gttggtgcag ctgtaaagta tcagttatta ccgagtgtga ttctttctca
    30361 attaggctat gaatcagctt atggacagtc acaatctgga agaaacgata cgaacttttt
    30421 tgggattacc tggtttcctg gttgtcccta cccacaaggt tctgcacgtg gaattggtgg
    30481 ttctgaaggt ggtttctata tgaagtttcc gaacttaaaa gcttgttatg actattatgg
    30541 ctttatggtg gcgagccaaa gcaactttaa tcaatgtgtg ggcaataaag atcctggaag
    30601 ttgtttattg attctaggtc gtggaggcta tgcggcagca ggtattaccg aagggagtcc
    30661 gtattatcga ggagcaatga gtatcataac gtcctataac ttaacggaat atgatacttt
    30721 tgcgatcaag cattggaata gcctaccaac aaaaaaaact tcgcttattg caggaaaaag
    30781 taaaggcaat gcgaaggttt tggatcggat cattggacga tccgtaaata atggggaatg
    30841 ttatggtggt acggcatggt atgtatctca attaggtggc ccacagttaa tgggttctgg
    30901 acacatgttt gcgatgttaa taggggaaga ttataactgg acaacctatg gttggcaagt
    30961 aatcatgaac ccaaaaccaa gtgatttaca agcaggagat gtggtgaatt ggcaagcagg
    31021 tggtgcattg tctcctggaa tctatggtca caccggaatt ataacagatg tttcatatgg
    31081 cggacaacat ttctctagtg tggaacagaa tgccgagcaa ggacggatta tggctcggta
    31141 tcaacgcacg tacaatcaaa caaaaattcg tagtctggtt cgtaaggtga aataagaaag
    31201 gcagtgacta gaaacgtgaa aaaagggata attttagtat ttagttggtt atgtttgata
    31261 ggtcttactg gttgtcattg gttttcttct gaaaaacaaa aaacacaatt gagaacgaca
    31321 gaagaaacaa tcgaagcagt aaaagaaaca agtggaactc ttcaaagtac tgaaagtaac
    31381 agtcaaattg gttcattaga agacgagaca gcgcaacaaa tgcaaataga tgaaatgaca
    31441 aaaaatttct taaaagattt tgctagaaaa tggagtaatt tcacagatgt ttataaacgc
    31501 aatcaagctg tgagggacta tatgacagat cgttgtatta aagacaatag tattgatgta
    31561 gacccgcatg tacaaatgaa aacaactggg aaactttacc aggtgacaca agatatagag
    31621 aatccggatc aatatttgtt atttggagaa gaaacaatga ataaggttac tcaatttgta
    31681 ttacttcaat tgaaagtaga tcaagaagct caaaagattg atgcgatcaa gatttattat
    31741 gtacggagtg cgtattaaaa taaaaaatga acactcatat ttattataca ccgaagaaat
    31801 agaaaaaaac agactgtttt tttaagaaaa actatgtcat tctttacttg aaaaaagttg
    31861 ttataggaca gaaaggataa aacaaaaacg gaaaaaatat gtcttatgaa taactataag
    31921 aactattcta ttttttagga gatggatacg agatgaaaaa actaattggc tatagtgtga
    31981 tgtgtattgg atttgttggt gtgggattgt ttgtatcgac tgaatctgct tctgcggctg
    32041 aatggtataa tcctatctgg tataatccga acattgaatg tcaaaaaggc tatccgtatg
    32101 gaccgacaaa tctgaaaaat ggaaaatgta gagtaaattg gaatggcgtt gtagatagtg
    32161 taattactgg ttcaatggac tctgctctac atggctttac gggtcactag aaggaggaag
    32221 aaaatgtctg aagagaaaaa agaaatcgtt gaatcattag tagctttaaa agattctctt
    32281 tctaagattg aatatgttga taggcaagaa attcaaaaac taattgatga tactattatt
    32341 gaaatccaag atgcgcgttg tgaaggtata aaaatttctg tagctttatc taaagtaata
    32401 gaaaaaatga accgttcatt agcgtttaat ggattgaagc tagatagaca aactagtctg
    32461 atatgggatc atttaaaaga cttgtatgac aagtctaaaa gaagtgaacg gactgctgta
    32521 tcaatcttaa aaggcctttg gggaatgaat tc
//
LOCUS       DBKOIJ010000013        31726 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000013 DBKOIJ010000000
VERSION     DBKOIJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.013"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(74..319)
                     /locus_tag="ACV5NJ_000490"
     CDS             complement(74..319)
                     /locus_tag="ACV5NJ_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HHY6484616.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(434..1012)
                     /locus_tag="ACV5NJ_000491"
     CDS             complement(434..1012)
                     /locus_tag="ACV5NJ_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HHY6484617.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1089..1619)
                     /locus_tag="ACV5NJ_000492"
     CDS             complement(1089..1619)
                     /locus_tag="ACV5NJ_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HHY6484618.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1623..2483)
                     /gene="rlmB"
                     /locus_tag="ACV5NJ_000493"
     CDS             complement(1623..2483)
                     /gene="rlmB"
                     /locus_tag="ACV5NJ_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HHY6484619.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2476..2871)
                     /locus_tag="ACV5NJ_000494"
     CDS             complement(2476..2871)
                     /locus_tag="ACV5NJ_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HHY6484620.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2868..4280)
                     /gene="cysS"
                     /locus_tag="ACV5NJ_000495"
     CDS             complement(2868..4280)
                     /gene="cysS"
                     /locus_tag="ACV5NJ_000495"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HHY6484621.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4277..4813)
                     /gene="epsC"
                     /locus_tag="ACV5NJ_000496"
     CDS             complement(4277..4813)
                     /gene="epsC"
                     /locus_tag="ACV5NJ_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HHY6484622.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4899..5138)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5135..6595)
                     /gene="gltX"
                     /locus_tag="ACV5NJ_000497"
     CDS             complement(5135..6595)
                     /gene="gltX"
                     /locus_tag="ACV5NJ_000497"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HHY6484623.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6765..7922)
                     /locus_tag="ACV5NJ_000498"
     CDS             complement(6765..7922)
                     /locus_tag="ACV5NJ_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HHY6484624.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7956..9326)
                     /gene="radA"
                     /locus_tag="ACV5NJ_000499"
     CDS             complement(7956..9326)
                     /gene="radA"
                     /locus_tag="ACV5NJ_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HHY6484625.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9389..9916)
                     /locus_tag="ACV5NJ_000500"
     CDS             complement(9389..9916)
                     /locus_tag="ACV5NJ_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HHY6484626.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10146..10952
                     /gene="proB"
                     /locus_tag="ACV5NJ_000501"
     CDS             10146..10952
                     /gene="proB"
                     /locus_tag="ACV5NJ_000501"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HHY6484627.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10949..12196
                     /locus_tag="ACV5NJ_000502"
     CDS             10949..12196
                     /locus_tag="ACV5NJ_000502"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HHY6484628.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12272..12616
                     /locus_tag="ACV5NJ_000503"
     CDS             12272..12616
                     /locus_tag="ACV5NJ_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484629.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12733..13059)
                     /locus_tag="ACV5NJ_000504"
     CDS             complement(12733..13059)
                     /locus_tag="ACV5NJ_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484630.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13432..14633)
                     /locus_tag="ACV5NJ_000505"
                     /pseudo
     CDS             complement(13432..14633)
                     /locus_tag="ACV5NJ_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14750..15265
                     /locus_tag="ACV5NJ_000506"
     CDS             14750..15265
                     /locus_tag="ACV5NJ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HHY6484631.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15464..15910)
                     /locus_tag="ACV5NJ_000507"
     CDS             complement(15464..15910)
                     /locus_tag="ACV5NJ_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HHY6484632.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15957..17435)
                     /gene="msr(C)"
                     /locus_tag="ACV5NJ_000508"
     CDS             complement(15957..17435)
                     /gene="msr(C)"
                     /locus_tag="ACV5NJ_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HHY6484633.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17789..18418)
                     /locus_tag="ACV5NJ_000509"
     CDS             complement(17789..18418)
                     /locus_tag="ACV5NJ_000509"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HHY6484634.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(18402..18866)
                     /locus_tag="ACV5NJ_000510"
     CDS             complement(18402..18866)
                     /locus_tag="ACV5NJ_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHY6484635.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19353..20621)
                     /locus_tag="ACV5NJ_000511"
     CDS             complement(19353..20621)
                     /locus_tag="ACV5NJ_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HHY6484636.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20852..21589)
                     /locus_tag="ACV5NJ_000512"
     CDS             complement(20852..21589)
                     /locus_tag="ACV5NJ_000512"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHY6484637.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21656..22225)
                     /locus_tag="ACV5NJ_000513"
     CDS             complement(21656..22225)
                     /locus_tag="ACV5NJ_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HHY6484638.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22353..23225
                     /locus_tag="ACV5NJ_000514"
     CDS             22353..23225
                     /locus_tag="ACV5NJ_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHY6484639.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23567..24439
                     /locus_tag="ACV5NJ_000515"
     CDS             23567..24439
                     /locus_tag="ACV5NJ_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HHY6484640.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24436..26394
                     /gene="nagE"
                     /locus_tag="ACV5NJ_000516"
     CDS             24436..26394
                     /gene="nagE"
                     /locus_tag="ACV5NJ_000516"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HHY6484641.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26645..27496)
                     /locus_tag="ACV5NJ_000517"
     CDS             complement(26645..27496)
                     /locus_tag="ACV5NJ_000517"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHY6484642.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27619..28086
                     /locus_tag="ACV5NJ_000518"
     CDS             27619..28086
                     /locus_tag="ACV5NJ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HHY6484643.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28349..28831)
                     /locus_tag="ACV5NJ_000519"
     CDS             complement(28349..28831)
                     /locus_tag="ACV5NJ_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HHY6484644.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            29043..30122
                     /locus_tag="ACV5NJ_000520"
     CDS             29043..30122
                     /locus_tag="ACV5NJ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HHY6484645.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(30321..31184)
                     /locus_tag="ACV5NJ_000521"
     CDS             complement(30321..31184)
                     /locus_tag="ACV5NJ_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHY6484646.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9064 a         6783 c         5848 g        10031 t
ORIGIN      
        1 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
       61 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
      121 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
      181 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
      241 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
      301 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
      361 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
      421 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
      481 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
      541 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
      601 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
      661 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
      721 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
      781 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
      841 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
      901 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
      961 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     1021 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     1081 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     1141 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     1201 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     1261 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     1321 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     1381 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     1441 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     1501 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     1561 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     1621 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     1681 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     1741 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     1801 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     1861 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     1921 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     1981 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     2041 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     2101 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     2161 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     2221 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     2281 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     2341 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     2401 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     2461 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     2521 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     2581 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     2641 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     2701 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     2761 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     2821 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     2881 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     2941 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     3001 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     3061 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     3121 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     3181 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     3241 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     3301 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     3361 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     3421 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     3481 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     3541 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     3601 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     3661 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     3721 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     3781 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     3841 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     3901 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     3961 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     4021 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     4081 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     4141 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     4201 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     4261 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     4321 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     4381 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     4441 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     4501 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     4561 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     4621 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     4681 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     4741 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     4801 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     4861 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     4921 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     4981 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     5041 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     5101 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     5161 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     5221 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     5281 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     5341 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     5401 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     5461 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     5521 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     5581 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     5641 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     5701 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     5761 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     5821 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     5881 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     5941 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     6001 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
     6061 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
     6121 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
     6181 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
     6241 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
     6301 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
     6361 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
     6421 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
     6481 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
     6541 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
     6601 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
     6661 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
     6721 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
     6781 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
     6841 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
     6901 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
     6961 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
     7021 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
     7081 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
     7141 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
     7201 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
     7261 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
     7321 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
     7381 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
     7441 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
     7501 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
     7561 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
     7621 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
     7681 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
     7741 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
     7801 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
     7861 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
     7921 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
     7981 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
     8041 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
     8101 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
     8161 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
     8221 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
     8281 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
     8341 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
     8401 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
     8461 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
     8521 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
     8581 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
     8641 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
     8701 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
     8761 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
     8821 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
     8881 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
     8941 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
     9001 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
     9061 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
     9121 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
     9181 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
     9241 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
     9301 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
     9361 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
     9421 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
     9481 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
     9541 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
     9601 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
     9661 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
     9721 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
     9781 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
     9841 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
     9901 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
     9961 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    10021 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    10081 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    10141 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    10201 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    10261 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    10321 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    10381 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    10441 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    10501 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    10561 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    10621 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    10681 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    10741 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    10801 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    10861 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    10921 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    10981 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    11041 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    11101 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    11161 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    11221 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    11281 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    11341 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    11401 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    11461 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    11521 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    11581 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    11641 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    11701 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    11761 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    11821 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    11881 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    11941 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    12001 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    12061 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    12121 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    12181 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    12241 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    12301 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    12361 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    12421 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    12481 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    12541 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    12601 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    12661 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    12721 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    12781 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    12841 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    12901 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    12961 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    13021 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    13081 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    13141 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    13201 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    13261 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaaa
    13321 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagaagct atttttgttt
    13381 ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc
    13441 ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat
    13501 ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat
    13561 aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt
    13621 tcgttgcaga cctagtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg
    13681 gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc
    13741 taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt
    13801 aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca
    13861 gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata
    13921 cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag
    13981 tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc
    14041 agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc
    14101 aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc
    14161 cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa
    14221 aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc
    14281 ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac
    14341 aacgatgata aacaatcctg tcatcaatgt tttttcaaac cgatccgact agataagaaa
    14401 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
    14461 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
    14521 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
    14581 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
    14641 tctctattag catcatacat gatttatttt acatgtaaat atgcacatgt aaaacgtgga
    14701 aactttactg tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt
    14761 agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca
    14821 gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt
    14881 agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta
    14941 ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc
    15001 tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac
    15061 aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa
    15121 tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt
    15181 gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga
    15241 acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga
    15301 agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg
    15361 acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca agagactatg
    15421 acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa
    15481 ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg
    15541 caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt
    15601 gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg
    15661 aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt
    15721 ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca
    15781 ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc
    15841 tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat
    15901 ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa
    15961 aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc
    16021 gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc
    16081 attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac
    16141 agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc
    16201 ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc
    16261 aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat
    16321 tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag
    16381 agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca
    16441 ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt
    16501 aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg
    16561 agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt
    16621 tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt
    16681 acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg ctttttcttt
    16741 ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc
    16801 aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat
    16861 tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt
    16921 acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc
    16981 agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt tctatctaga
    17041 tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac
    17101 tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac
    17161 tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga
    17221 ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg
    17281 gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga
    17341 tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact
    17401 tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta
    17461 aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc
    17521 acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt
    17581 tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta
    17641 tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat
    17701 gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa
    17761 tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt tctggtgcta
    17821 aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa
    17881 cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa
    17941 ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc
    18001 tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa
    18061 tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt
    18121 ggttatagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg
    18181 cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg
    18241 cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa gaaggtgcag
    18301 tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat
    18361 atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta
    18421 ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa
    18481 agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta
    18541 tctttatggt ccatttcaca atgctcgatt ttcttctggg ctttttctgt tagcgctaca
    18601 tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat
    18661 accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt
    18721 tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata
    18781 ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa
    18841 aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg
    18901 attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa
    18961 aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa
    19021 aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa
    19081 gatagctctt ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat cgccttttgc
    19141 aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta
    19201 tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg
    19261 taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac
    19321 ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca
    19381 tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg
    19441 gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa
    19501 aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc
    19561 attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg
    19621 tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt
    19681 tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc
    19741 ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg
    19801 ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca
    19861 tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga
    19921 aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat
    19981 tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc
    20041 gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa
    20101 gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca
    20161 tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct
    20221 tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca
    20281 gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt
    20341 tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt
    20401 tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct
    20461 gactgcaaag tactctctgt atcgttttca attcctgttt ctgaagaact cgatgtctga
    20521 gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt
    20581 cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt
    20641 atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat
    20701 ttaaataaca tttgtttctt tttgataaat ttgctttttc tacataaaaa gacgtaaatg
    20761 aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa
    20821 gtaattctct ttggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa
    20881 tcatgttact gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta
    20941 tatcttcgga ggtcaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt
    21001 tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt
    21061 tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt
    21121 ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg
    21181 aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta
    21241 acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta
    21301 atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt
    21361 ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa
    21421 tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg
    21481 cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc
    21541 cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct
    21601 cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa
    21661 taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg
    21721 ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc
    21781 ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta
    21841 ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac
    21901 gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt
    21961 gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag
    22021 gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc
    22081 ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttcag
    22141 cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga
    22201 tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga
    22261 cggcttatca tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag
    22321 tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt
    22381 tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac
    22441 ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc
    22501 ggggctcccg agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag
    22561 aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg
    22621 gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc
    22681 attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg
    22741 atatcataga caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa
    22801 acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac
    22861 atcgaaatca tcctttagca aagcaaaccg tcatccatcc gcaggaactg aaaactgttc
    22921 ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca
    22981 gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg
    23041 tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg
    23101 aaaaaatatt catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa
    23161 aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca
    23221 gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag ataagcaaaa
    23281 gatccaacga ttaaataaca taagtttctt aatttatttc ttccttttat ttctgataat
    23341 tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa
    23401 ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct
    23461 ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa
    23521 ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa
    23581 gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg
    23641 aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg
    23701 gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga
    23761 tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga
    23821 aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa
    23881 cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag
    23941 tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc
    24001 atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg
    24061 gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt
    24121 ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact
    24181 ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg ctgttttaga
    24241 cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa
    24301 aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta
    24361 cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca
    24421 tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca
    24481 gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat
    24541 agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta
    24601 cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct
    24661 gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta
    24721 gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac
    24781 gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa
    24841 gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct
    24901 gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc ggtttactct
    24961 gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc aggtctttat
    25021 ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa ctccgtgttc
    25081 tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga aggaacaaaa
    25141 ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg gcttccagca
    25201 ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg
    25261 atgctagcag cagcttttgc ttcattcttt actggtgtga cagaaccact tgaattttca
    25321 tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg gatttcttta
    25381 gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg atttgtcgat
    25441 tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat cgtccaagga
    25501 ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa attccatctg
    25561 atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac gactaattca
    25621 gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg agccgacaat
    25681 gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga tacaggcctg
    25741 atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat cgacggaaaa
    25801 aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat ggcaaaacta
    25861 catggtggtg ctgcagcacg acctgcacaa acaaacaccg tggttaaaac agaaacattt
    25921 gccggtgaaa cgatcgaaca agatatttac gctatcgcaa atgggaaact gattccaatc
    25981 actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta tgctgtatta
    26041 ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat tttcccaacg
    26101 aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca tatgggcatc
    26161 aacactgttg acttaaaagg cgaaccattc actttatatg tagaagaagg tcaaaaagtc
    26221 gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc aggcaaaaac
    26281 acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc
    26341 gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc ctgagccatc
    26401 gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa tcttgtctca
    26461 gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat aagtccagct
    26521 aaacgaaaca tatgaagagc tattttttat ttgactcttc ttattacttg atgcagaaca
    26581 gctttttcac aaaatttttc ttcatactca aaactaatcg gctttttcac aacctcttta
    26641 tacattattc ctgatccaat atttctttta atgctgcttc aagagaagtt aatggttttc
    26701 ccaatacttt ttctaaatca tcagaggaaa tagctaactg atccttttta atatcatgtt
    26761 ggatcgctaa aaacatgtca gctgcttctt gagggaatcc ggcttctttt agattttcga
    26821 caaacgctgc atcacttgct tcaataattt ctaactcttt acctgtcgca cgctctaatg
    26881 ccttggctaa ttcttcatag tcagttagat tgccggataa ttctaagatt tctggagagt
    26941 cactttctac tagtactttt gctgcagctt ctgcatattc acgtttcaat gcccaacctg
    27001 tttttccatt tcctgcagca taaacgaatt gaccagtttt taaagcctgc ccaatgatag
    27061 gtaattcatt ctctacatac caattattcc gtaaaaatgt atgggaaata cctgaatcct
    27121 taatcatttt ttcagtgaat tggtgatctg gcgctaatac actgatcgag tgatcggcat
    27181 ctgcaaaact tgtataagcg atataagata ccccagctgc ttgtgctgcc ttcactacat
    27241 tcttatgttc ggcttgacga tttcctggaa cgccagagac aaaaagcaat cgatctattc
    27301 ctttcaacgc ttctttcatt gagtctaaat catcataatc accaatacgt atattaaatc
    27361 cagcttcttt cagtgcttct cctttttctt cactacgtac tagtacatag atttcatttt
    27421 gtggaaccat cttttttaag aattccagcg cataaccacc aaaacctcca gttgctcctg
    27481 ttactaaata atgcatattt gccactccct attcttttta tttgttaaga tttacagctg
    27541 tacatttata aattacacct ttatcataaa gagtgtggta agataagtca agcaaaaaaa
    27601 atgaaaggat gacaactgat gaagtattct ctgcaattta gtgatgcaat ccatgtactt
    27661 gcctatattg aaatgtttca agatacccga tcactttcta gtgagatgat tgccagtagt
    27721 atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg
    27781 atctttacaa aaaccggaaa accgcagcct tcccttgcca aaagtccaga tcagatttct
    27841 ttactagatg tctatcgcag catcgaaggt aatacacagc ttatccaagt cgatccaaaa
    27901 acaaatccaa attgcgtcgt tggtgcgaat atccaagaag ctttagaaga aagctacgct
    27961 cggctgcaga aaaaggcaga agaagaaatg gctaagatca cacttgatag tctgatacat
    28021 aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt agagagattt
    28081 ttataattct ctctactgtg cctgcatttt tctgatcgga atcgtctaca taaattcttt
    28141 tattctccgg gggacaaaag ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa
    28201 agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt agctgaccct cggcattagt
    28261 tctgaaaaat gcaaaatatg gaaagctatt tcccattttt cctctctaat gctcaggtca
    28321 aagcgctact ttcacgacct ctatattatc atggtttatt gactaccggt atccagattt
    28381 ctcccatata tttcccgttg gtaactccca tttcgactac agcatttgga cccccaacat
    28441 aggcgtaatc ttctacttct cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc
    28501 ttaattcttc aggcgattct gctaaagcgc ttacaacaag atacgttcct tttgggaatg
    28561 ggatgattcg cgtagtgttt ggaacatcct gactcgtttg aacaccgaca tagtacatca
    28621 ttttcccgtt atagggttcg ttgactgtaa agaaataatg attttcagca gcttgtttca
    28681 atgcatctat tgttccatta ccgataactt tttgctcaaa tgcttgtttc tctgcagcca
    28741 aaccgacaaa atccgtattg ctactcttca attccacacc aatacccgtc accgtaaaat
    28801 ctggtttttc ttcgatccta tactttgcca tttctcccat cctttctatt atattaagtt
    28861 catcttacat aaaattaaag ggaaatgaca cgattacgct cttttttcta gggatatttt
    28921 tattttttaa ctatttggcg gattttataa ttaacttagc catttggaaa taaaaaaacg
    28981 ccacgtgaat tccatgttat attgaagtta ccacaactct caatagacaa ggatgaattc
    29041 acatgacgca aaaccatgat aacacagttt cacgcaaggg aaaacaccta acctactcag
    29101 aacgttgcca aatcgccatc ctgaaaaagg aaaactattc caaccgtcag gttgcggatg
    29161 tcctgggctg cgtgccgcag accataaaca acgaaatcaa acgcgggacc gttactcagc
    29221 ttaaccgcca gaatcagaac gggaaggtct acgactatta tcatgacgtc tacgacgccg
    29281 atgccgggca ggcggcctac gagaagaacc gcttgaactg cggcagacgg cccttatggg
    29341 ccgacacgga cgccttcatt gaatgggcgg acgacatgat gctgttggaa aaatggtccc
    29401 cggacgtggt ggtgggcttt gccagaaaac acgaattgtt cgacccggcc attatccctg
    29461 ggaccaccac cctctatcag tggatcgacc gtggcatcat gaaaacgaag aatatggacc
    29521 tgctagaaaa actgtcccgc aaaccgaaat cgtcccgtca tggcaaccgc ccgaataaac
    29581 gggtgctagg gaaatccatt gaagagcgcc cggaggcggt tggtacccgg aaaaccttcg
    29641 gccattggga agtcgacacg gtcgtaggaa acaagaccaa gtcggatgcc gtgctgctga
    29701 cgttggtgga acggcagaca cgcttcgagg tcattctgaa gctggccggg aaggatgccg
    29761 aatcggtcaa ccaagcggtc cgacagctga gagaacgggc tggtgcgtac ttcggccagc
    29821 tgttcaagac ggttacctcg gacaacggct cggagttcgc gggactgcac gatgccttgc
    29881 aggatgtggc aaacatctac ttcagccacc cctatgcttc ctgggagcgc gggacgagcg
    29941 aaaaccaaca caagctgatt cgccgtttca tccccaaggg cagcccaatc ggcaacgtga
    30001 gcgaaaacca aatcttgcgg attcagcgtt ggatgaacga ttacccgcga aagattttgg
    30061 gctatgcaac gccgcatgac acgttcgtcc aggcatttag gcaggaacgg ttgacggctt
    30121 gacacgtggg cccttgacaa cgagcctccc tcggcatggc ttgtgtctgg aaagctggcc
    30181 ggggccagcc agtcgggaga ctaccatgcc tcagctcatt gtcaagggcc cactcgatta
    30241 gaaaaattag acggttggtg gtaacgagca accgggtcac catgggtggc taacttaaac
    30301 ttgaaattta cgttttttaa ctattttttt gctttcactg caatccaaat ctcactgtaa
    30361 acattactga aatcctctcc ccattttgta aaagagatct cgggagcttc gactaaccca
    30421 taatctgaag aaggaagcca ttctgcatag attttccccc aagtatcttg taaagtttgg
    30481 ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt cttctatagc aagcatttct
    30541 aaatcagcgt acggattttc ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc
    30601 ttttcttcca agcggtctcc atcaaatcca taagaaacgt tgattacttg atttggatat
    30661 aaatctccta acttatgcat ctcttccctt tgagtctccg tgatcgtctg cgctaacttt
    30721 tgtatttctt gattgactcc ttcaaatttg acaggaactt ttttcgccac acctgccaca
    30781 taaaactgtt ctttcttttc gatacgaaat tccatcgatt ttcctcctct gacatcaata
    30841 aaaaatgtca agcgtggaaa agatttttgt atgccgtatc tactgatttc tgaaggcaga
    30901 tatcctgacc aatcacgaaa agctcttgaa aaaccttcga ctgtttcata tccatatcta
    30961 aacgccgttt ctgtcactct tgatccatct agaagatcgc gattagccaa agccaatcta
    31021 cggtttttga tatagtccat tagcgacatc cctgcaataa acgtaaagat gcgttttaat
    31081 tgatattcag agattcctgt attttcggct attattttat aggaaagtcc ttctgtcaaa
    31141 tgttcttcga tgtaatcgat caattggttc agatctttaa gcatatttat cttcctcctt
    31201 gattgccttt ttatcatatt atcaaagaaa gtcaattact tgatgaatca ggaaccaatt
    31261 tagtcagatg tagaaaatgt tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa
    31321 cggataattg gtattatctg cctatctatg ttttaccttt agaaaaataa attttgggga
    31381 agacagattc taagtaaaca aagcggtaaa aatatctttt attcccttag ttaatccgct
    31441 tactacctac tgtacaaagc aaattgttgt tcaaactgct gatcagaacg ctgcttggat
    31501 ttttctttgc gatacatcat acctctttta tcaattggaa caattattta tttctaacaa
    31561 aatcacttca tttttgtcag ataaataaaa aacaaaaaaa gttccatctt taaaacacgc
    31621 tattctttta ctttttctca cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa
    31681 ttcgagcaaa agtgattatc aaatatgtaa aatcagctac tttttt
//
LOCUS       DBKOIJ010000014        31547 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000014 DBKOIJ010000000
VERSION     DBKOIJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.014"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..1107)
                     /gene="tgt"
                     /locus_tag="ACV5NJ_000522"
     CDS             complement(<1..1107)
                     /gene="tgt"
                     /locus_tag="ACV5NJ_000522"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HHY6484647.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFE"
     gene            1325..2023
                     /locus_tag="ACV5NJ_000523"
     CDS             1325..2023
                     /locus_tag="ACV5NJ_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HHY6484648.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2191..4830)
                     /locus_tag="ACV5NJ_000524"
     CDS             complement(2191..4830)
                     /locus_tag="ACV5NJ_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HHY6484649.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(5449..6480)
                     /gene="queA"
                     /locus_tag="ACV5NJ_000525"
     CDS             complement(5449..6480)
                     /gene="queA"
                     /locus_tag="ACV5NJ_000525"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HHY6484650.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            6673..7847
                     /locus_tag="ACV5NJ_000526"
                     /pseudo
     CDS             6673..7847
                     /locus_tag="ACV5NJ_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            7851..8492
                     /locus_tag="ACV5NJ_000527"
     CDS             7851..8492
                     /locus_tag="ACV5NJ_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484651.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            8489..9406
                     /locus_tag="ACV5NJ_000528"
     CDS             8489..9406
                     /locus_tag="ACV5NJ_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HHY6484652.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            9403..10074
                     /locus_tag="ACV5NJ_000529"
     CDS             9403..10074
                     /locus_tag="ACV5NJ_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484653.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(10615..11103)
                     /locus_tag="ACV5NJ_000530"
     CDS             complement(10615..11103)
                     /locus_tag="ACV5NJ_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HHY6484654.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11304..11570)
                     /locus_tag="ACV5NJ_000531"
     CDS             complement(11304..11570)
                     /locus_tag="ACV5NJ_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HHY6484655.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(11570..11833)
                     /locus_tag="ACV5NJ_000532"
     CDS             complement(11570..11833)
                     /locus_tag="ACV5NJ_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HHY6484656.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12354..12494
                     /locus_tag="ACV5NJ_000533"
                     /pseudo
     CDS             12354..12494
                     /locus_tag="ACV5NJ_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(12636..14459)
                     /locus_tag="ACV5NJ_000534"
     CDS             complement(12636..14459)
                     /locus_tag="ACV5NJ_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HHY6484657.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15057..17762)
                     /locus_tag="ACV5NJ_000535"
     CDS             complement(15057..17762)
                     /locus_tag="ACV5NJ_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HHY6484658.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18068..19015)
                     /locus_tag="ACV5NJ_000536"
     CDS             complement(18068..19015)
                     /locus_tag="ACV5NJ_000536"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HHY6484659.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19205..19474
                     /gene="rpsN"
                     /locus_tag="ACV5NJ_000537"
     CDS             19205..19474
                     /gene="rpsN"
                     /locus_tag="ACV5NJ_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HHY6484660.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(19650..20012)
                     /locus_tag="ACV5NJ_000538"
     CDS             complement(19650..20012)
                     /locus_tag="ACV5NJ_000538"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HHY6484661.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20023..21144)
                     /gene="alr"
                     /locus_tag="ACV5NJ_000539"
     CDS             complement(20023..21144)
                     /gene="alr"
                     /locus_tag="ACV5NJ_000539"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HHY6484662.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21160..21510)
                     /gene="acpS"
                     /locus_tag="ACV5NJ_000540"
     CDS             complement(21160..21510)
                     /gene="acpS"
                     /locus_tag="ACV5NJ_000540"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HHY6484663.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(21640..23151)
                     /gene="cshA"
                     /locus_tag="ACV5NJ_000541"
     CDS             complement(21640..23151)
                     /gene="cshA"
                     /locus_tag="ACV5NJ_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="degradosome RNA helicase CshA"
                     /protein_id="HHY6484664.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(23473..24825)
                     /locus_tag="ACV5NJ_000542"
     CDS             complement(23473..24825)
                     /locus_tag="ACV5NJ_000542"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HHY6484665.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(24978..26054)
                     /locus_tag="ACV5NJ_000543"
     CDS             complement(24978..26054)
                     /locus_tag="ACV5NJ_000543"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HHY6484666.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26250..27995
                     /locus_tag="ACV5NJ_000544"
     CDS             26250..27995
                     /locus_tag="ACV5NJ_000544"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HHY6484667.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28275..28712)
                     /locus_tag="ACV5NJ_000545"
     CDS             complement(28275..28712)
                     /locus_tag="ACV5NJ_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HHY6484668.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(28696..30069)
                     /locus_tag="ACV5NJ_000546"
     CDS             complement(28696..30069)
                     /locus_tag="ACV5NJ_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484669.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(30391..30879)
                     /locus_tag="ACV5NJ_000547"
     CDS             complement(30391..30879)
                     /locus_tag="ACV5NJ_000547"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6484670.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(30901..31503)
                     /locus_tag="ACV5NJ_000548"
     CDS             complement(30901..31503)
                     /locus_tag="ACV5NJ_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HHY6484671.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9042 a         6742 c         5848 g         9915 t
ORIGIN      
        1 ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt gacgtacttg
       61 tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt
      121 ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac atgtataaca
      181 atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt tcactactag
      241 acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca agacacagtc
      301 aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac cgacacccat
      361 caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata cacgattcat
      421 ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga aatccatccc
      481 gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt ggatgattcc
      541 aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg cccatcgaga
      601 cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg gacattcgtc
      661 aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt tttctggtga
      721 taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt cttcgatatt
      781 ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc
      841 ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac cagggcgtag
      901 ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca actcttctgg
      961 ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg tggggaaagt
     1021 tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt tcttgattaa
     1081 acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa aatatcttga
     1141 caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg cttccttcta
     1201 ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa agaaaaacat
     1261 tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa aggagggaaa
     1321 aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg
     1381 gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta ctattcttct
     1441 tattatgatt cctattatct tgtttgccac caccatatat gatggatcag cgagcgtctc
     1501 agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta tttccagcat
     1561 cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt tacgtggaca
     1621 aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg
     1681 cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg gtttgctatt
     1741 agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta tttactacga
     1801 tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg caagtcgaaa
     1861 attgatggat ggttataaag gacaactttt ctggttagat ctttcattta tcggctggca
     1921 tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat acatttctgc
     1981 aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag tgtgacttgt
     2041 gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa acattcagcc
     2101 cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt atagttccag
     2161 taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt ccggtaatcg
     2221 atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc ctaccaagaa
     2281 ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc
     2341 aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt tattggtcgc
     2401 taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca gaatcgatga
     2461 ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt ctacccatgg
     2521 gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga tggtgaagac
     2581 ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt catttttcgg
     2641 aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag caaatcctgg
     2701 aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg gtgctcccca
     2761 tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc ttagcaaaaa
     2821 gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag cgtctacgat
     2881 cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca catctgttcc
     2941 tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat cattcacacc
     3001 atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga tccggatctt
     3061 gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat ctaattcttc
     3121 gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat tcaagatacc
     3181 taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca tgactgtttt
     3241 gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg gtggatcgat
     3301 cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag aaatatcttg
     3361 cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc gtttaccaaa
     3421 gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac
     3481 atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa ctgttgtcat
     3541 catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt cttctaattc
     3601 tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg ttggattacc
     3661 tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata gtgcagccat
     3721 tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta cttcatcttc
     3781 tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg ttttgtctga
     3841 gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga tgatcgcatg
     3901 tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt
     3961 ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc
     4021 tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga tcatactgat
     4081 tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag tattttcgtt
     4141 caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga cgatcgcttt
     4201 cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt cacctaatga
     4261 ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt ctccagtcaa
     4321 cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc
     4381 atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact caacagtggt
     4441 cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca aggattccag
     4501 tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca caacgatagc
     4561 gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg tataaaagga
     4621 aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt
     4681 tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct tttttcgccg
     4741 ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta ccttctttac
     4801 ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc ttcgtacaaa
     4861 aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa agggcttatc
     4921 gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg ctaccaaagg
     4981 atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag aatttctcga
     5041 tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga catgcgtgta
     5101 gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag tatccgaaac
     5161 aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt gaataggaac
     5221 aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta acatgaagaa
     5281 attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt ttatttgaat
     5341 ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt tgtcataaaa
     5401 tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt atttcacgaa
     5461 catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct ggtaagcaga
     5521 taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg attttggcaa
     5581 atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg taatgaaaat
     5641 atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa tcgtttctaa
     5701 tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac gtacttggtt
     5761 caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc
     5821 aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg tcagatagac
     5881 taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat gcaatccagc
     5941 tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc gatctgggtc
     6001 ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt caagaatctc
     6061 tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca attcttcttt
     6121 cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt tggcacgttt
     6181 cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa gaaggacttc
     6241 taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa ctcgtgtatt
     6301 attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga aatgtttatc
     6361 ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa
     6421 tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg ttgttaacat
     6481 atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg ttctgttggc
     6541 aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac taccctattt
     6601 gtattttttc attgttttag gcatactagt aatagatagt tcttcattga aaatagaaaa
     6661 agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc
     6721 gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt tatcggtacg
     6781 agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga tccaacacaa
     6841 gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga attgcgacgt
     6901 cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat ccgtgaaaat
     6961 atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa aacagctgaa
     7021 aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc acatgaatta
     7081 tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc
     7141 atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc cttgcaagac
     7201 cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac ccatgacatg
     7261 gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg tgtgatcaat
     7321 ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga tttcgtagaa gagttgattg
     7381 gagaagatcg cctactacaa gccaagccaa atgcgacaag agtcggcgaa gttatgctaa
     7441 atacagcaat cacgattaca ccggaaaaat cattacaaga agcaatcaaa ctgatgaggg
     7501 aaaaacgtgt agatacgttg ttagttaccg ataattcgaa tgtattaaaa ggatttatcg
     7561 atgtagaaac gttgaataaa aaacgaggaa aagtatccag tgtcggcgac attttgaata
     7621 aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga taccttgcaa cgcattttaa
     7681 aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa caaagtcgta ggtatcctga
     7741 ctcgtgcatc attagtggat atcgtctacg atgtgatttg gggagaagat cccgcaacta
     7801 ctgatgttgc agagtcagct actcctgaag aatctaagga ggtctgacct atgcagcagt
     7861 tttttcatca gcacggttca gaaattctct caaaaggttt tgaacatctt tatatttcag
     7921 gacttgcttt gatcattggt atcatcattg ctgtaccgct tggtgtctta ctgacacgtt
     7981 ttccaaaagc tgccacgatt gtgattggct taaccagtgc cttgcagaca atcccttctc
     8041 ttgctttatt ggcattgatg atcccgctat ttggcgtagg taaattacct gcgattatcg
     8101 cactctttat ttattcatta ttgccgattt tacgtaatac gtacattggg atgaaaaatg
     8161 tcgattggaa ttatcgggat gtggcaaaag gcatggggat gactgagttc caatccattt
     8221 tttccgttga attgcccatt gctatgccaa cgatcatggc tggtattcgt ttagctgccg
     8281 tttacgtgat tgcatgggct acacttgcct cttatatcgg cgctggtggt ttaggcgatt
     8341 tgatttttag tggactgaat aattaccaac cagatctcat tttggcaggt acgatccctg
     8401 taacaatttt agcactattg gctgattggc ttctaggtct cttggaacac cggctgacac
     8461 cgatcgcact tagagaggag aatgaagaat gaaacgtata ctaaaaatga ccatggttct
     8521 tttctctctt cttatacttg cgagttgttc cttccctggt cttgccagca atacagatga
     8581 agacaccatt tcgatcacag gtggaatcac ttcagaagca cagatcttag caagtctagt
     8641 tgccggaatg gttgaacatt atacagataa aaataccgca atcatcaaca atctggctac
     8701 tacaactatc aaccatcaag cgatgatgaa cggagatgct tcaatttcag cggctcgcta
     8761 cacaggaaca gatttaacga ctacattgaa ccttcctcct gaaaaagatc cgaaaaaagc
     8821 gtttgctacc gtgaaagatg aatttgagaa aagatacggt caaacgtggt tcccgtccta
     8881 tagttttgaa aatacgtatg tctttcttgt tcgaaaagac accgcacaaa aataccatct
     8941 atctaaagtc agcgatctga aaaatgtagc ggatgaatta gtagctggtg tagatacttc
     9001 ttggatcaat cgtaaaggag acggttatga cggcttccaa gaaacttatg gcttctcctt
     9061 caattcgata ttacctatgc agattggact tgtttatgac gcagtcgaag caggaaaaat
     9121 ggatattgtt cttggctact ctacggatgg acggatcgcc agttatgatc tagtcatgtt
     9181 ggaagatgat cgtcaattct tcccaccata tgatgctgct ccagttgtag acaacaagat
     9241 attagctgat acgcctcatt taaaagaagc cttaaataag ttaggtcata ccatcagtac
     9301 ggaaaaaatg caggaattga actacgaagc agataataat ctagtggaac catcagtagt
     9361 agcacaacgt ttcttacaag aaaaccacta ttttgaaggg aagtgaagca attggataaa
     9421 atgaatgtct ttcaacaatt tttctattat ttccaggaaa atggctccta tatctttgca
     9481 caatttatcc gtcacttcct gatttctatc tatggtgtcc tctttgcagc agttgtcggg
     9541 attccggttg ggatcatgat ttctaggaga cgtaaattag caaactgggt gattcgtttg
     9601 gcgaatatca tccagacgat cccttctcta gccatgatct ccattttaat cattggatta
     9661 ggcctgggag tcaatgtcgt gattgttact gtatttttgt attccctctt accgatcatc
     9721 aagaatacgt ataccgggat gatccaagta gacaaaaaca tccttgatgt cggcaaaggc
     9781 atggggatga ctgctagaca acgactattc atggtagagt taccactctc tgtttctgtt
     9841 atcatggcag gcataagaaa cgcgttagtt gttgccatcg ggataaccgc gatcggtgca
     9901 ttcgtcggtg ctggtggtct tggagatatc attatccgag gaaccaatgc aacagagggc
     9961 acatcgatta ttttagcagg tgcattgcca actgcattaa tggcgatcat cacggactgg
    10021 cttttaggta ttttggaaag acggctagat ccagccagcc gtacatcacg ctgatgttat
    10081 tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca aaggcttttt tagttctgtc
    10141 atttttaagg caataatcca ctctgattct tccctcctta tgggcaaaga gttgcttttc
    10201 ttagctattt atccattccg tatactccaa cttcatactg ccaacgggtt catatctgca
    10261 tccaccgact aagttttttt cttatccgct aaggcatttt gccaatccat tttcccatca
    10321 ctcgtcatgg aattcatacc acatgatctc taaatactcg tctgcttgtt ctttgaattg
    10381 ggcatcaatt cttacatcct ttggcgtggc tttcatttaa gccttttcca tcagtcctcg
    10441 attgttcgat gccttcagct gcatcatcaa aactggtgac tgccgtatcg gtaacttcga
    10501 tcatctttta tacaatcggg aacttgccat cctcatcctt ggctaaaaac atatctagcc
    10561 attccagctt ctcgccatgg gaggtttgcg gttcctaaaa ccggtacacg attcttatgg
    10621 actgcatccg tcgcaactag acttggcgat acgatgatcc ttcaccagaa gagcaccccc
    10681 gtaaaccaat tgatcgatat attgccaata agaaaagaca ttggcgtaaa agatatcgat
    10741 ccctcgtgat gcattgccgt tgatactgct attcatggcc gaaagcgcca cgactttaac
    10801 ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct ttggataccc gctctgctaa
    10861 aggaacgaca ctggtattgt atttcgtatc tgtatctttt ccaaaagacc atgccaacga
    10921 atcctctggg aaccaataag acgactccgg ttgattgtcc attgcttttt tcaacatact
    10981 tttggctgtt tctcgataaa cgacatttcc aattgtcttc gcctcacttt ggcgataatc
    11041 gatggtcgtt gccactgcca acactaacaa cgatgctcca attttactga ttttccttgt
    11101 cacacttctt cctccttgtt ttacataaac ccgtcaacat tccattccta aactcaaaca
    11161 atgacccttc aaaggtggag atataggaac aaattaaaga aatcatgcaa taaatccaag
    11221 aaggttggtt ttagaacaaa aaaagagtct cctttttggg gagactcttt gttaactatc
    11281 taaaaaggtt ggctcttctc acttcactca taatgtgtcc aggcagaata aatttctaca
    11341 actttatttt cctcatcaac tttgtaaacc actcgatgct gtctattcag cctgcgtgag
    11401 tatctgcctt catgtgtagg tctcagcttt tcaaaagact gcgtgggcat atatggatct
    11461 tccttcaatg tctgaatcac ttcttcaaat tgtttcttta aattcgtttg cttgattttt
    11521 cttaaatcca ctttcgcaga gtttttgatt gcgactgtat agttactcat caaagagctt
    11581 cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc tttttcgcgg tctcgtactt
    11641 tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg aatcgctctc cagtcttcca
    11701 gcccaatcaa tacagcattg tttccactac gatcactaat aatctcgatt tcagtatgat
    11761 tttcatttac ctctttcaat aactgataaa aatttttacg agcagtgcta ggattcatcg
    11821 ttgtattagc cattgtctct cctccttttt ctgatataat tatagcgtac ttgattaagt
    11881 acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac ttttcctctt ttttacatag
    11941 agaaaaagac ccctttttag ggaggatctt tgtccacgct cacttcttac tcctttacga
    12001 ctagcggtag ccaaacgtaa agtaattggt aacaacgtat catctttagc aaaggtcgaa
    12061 tccaaagcct gtgagtctat gcaacaacac caatacacga aggttcctcg ttgcagtgtg
    12121 acttgattga tatcttgaat aacctgagga tacgcacgaa agatttgtaa cggttattca
    12181 aaactgattt tggccgtcaa gtcttctact gcctcaaaaa tgattgcctc tttcgtctcc
    12241 tgtcattcat gttcactatc gatttgatac ttgcttagac cgttgggttt gtgaatcacc
    12301 aattaaaaat ggcactgagt tatggtaaca aaaaatggtc acttgacgag gctgagattc
    12361 ctacttatga agacacatgg cacttaagag tcgaggtaaa ggaagaagct gaaaatatag
    12421 gaggctgtac attatttggc aaaggattcg gaaattatga tcaagacatc aaaatcaaaa
    12481 tgttttacag ctaaatagga agaataataa catttcatac gctgttccca tctttaatgg
    12541 cttatagagg gcacctttag ctgagaataa gacaaaacag accctctagc ttcaattaag
    12601 agctagaggg tctgtttttg attttaaaac ttggtttatt tcttcaaatg gtagctatag
    12661 gtgctgacaa cgatattttc acgccaagac aatcgcaatc ttaaccgcaa acttgtttgg
    12721 ttatggagaa tattctgcca gcgtctgttt ggtacaaatt gaggaatcaa cacagtagtt
    12781 gtatagtttt gtttcgctgc atttttactt acgatatcta cataacggat aattggattt
    12841 tcaatcgagc gataagaaga gtggacgatt gaaaaacgga catctgggaa atgcttttta
    12901 aattctgctt cgatttcttt ttctttttcg acatcttcgt ctagcgaaac atgcatcgca
    12961 acgacataat caccgatcga acgtgcgtaa ttcaacgcac cgatatttac ccgtgtcaca
    13021 ttcccgacta atacaagaac ggtattcccg tcataatcat gtagttttgc atcattttct
    13081 aaacgaagct gttctgctac ttttttataa tgatcatgga tcttataaaa aatgaacata
    13141 acgattggca taatgatgaa gaaaggccaa atgtccccta accgatagac aaacaaaatc
    13201 gcaatgattg catatgagat gaacgcaccc gtaatattgg caatcgattt gcttaaccat
    13261 ctttttcctt cttttttcca tttgacgacc atccctgttt gagataaagc aaatggaatg
    13321 aacacgccga tagagtataa cggaatcaag cgttctgttg agccatggaa aataaacagc
    13381 aatacgatcg aacctagcgc tagtgtgata atcccattgg aatagcctag tcgatcccca
    13441 cgatcttgat acatgtgagg catgaattta tcttttgcca gattgtatgc aagaactgga
    13501 aaagcagaaa aaccggtgtt agcagcaact gctaaaatca gcgctgtagc aaattgaagg
    13561 acgtaataca aaataccatg tccaaagaca gctttaccaa tttgagacaa aacagtgact
    13621 tctttttcag gaacgatccc ataccaatag ttgataaatg tgatccccac gaagaagaaa
    13681 cctaagatca atgccatcat cgtcaatgta gcagcggcat tttttgctct tggcttctta
    13741 aagaatggta cagcattact aatcgcctcc accccagtta aagaggaaga accagaagaa
    13801 aatgctcgta aaatcaaagc gatagaaaca cccggcacag ccgctcctgg aagtgcagta
    13861 gcttgcaatg gcacagcacc tgtcacgata ttatataaac caacaacgat cagtaacgag
    13921 atcacaagga taaatgtgta caccggaaat aataggaacg atgcagattc cctcagccca
    13981 cgaagattca gcatcatcag tagcaaaacg attgtcacag aaatagcgac ttgatgaccg
    14041 tagagtgctg gcaccgctga tgtgatcgct tcagcacctg ctgagacaga tactgctact
    14101 gtcaacatgt agtcgatcaa taaagagcca ccggaaatca agccagcatt ctttccaaga
    14161 ttctcactgc tgacgacata tgctcccccg ccatgaggat aagcatggat gatttgacga
    14221 tatgatagcg ttaatgaaat caataaaata ataacaaaag cggcaatggg tagcgaatac
    14281 cagatggctg cagcagacaa agcgactaaa acgacaacga tttgttccgt accatatgca
    14341 atagatgata acgcatcaga cgacaataat gccaaagcag caaatcgggt taatttgtgc
    14401 tcatcatttt cagcagattt taaaggctta cctactaaca acctttttaa gtaatccaat
    14461 ttctaaactc ctttacacac aattttactt caagttttat agtcgaaaat ttatagtaaa
    14521 caagatgtta tagtacgctc actgacctta aaagtcaata aactttaaga aaataattgg
    14581 aaaaaaacag aaaaatatca agcgcttttt caaacgcttt aatacagcaa aaatctaaca
    14641 tttttttatg ggctttttct ttgaattcta taataaaagg atagaacttg acttagaaaa
    14701 tcaagttcta tcctttttga tatcttaact tttggaagag agatgtaagg ctgctttcta
    14761 cccatcattc ttactactcg aagcatagcg cagttgtcac aacctcttga taaacggtgt
    14821 ttaaaagctt gcatctgcgg taataagccc aaccaaatta aaaacataag aagctgtttt
    14881 tatttggtcg ttcttattac ttgatgcaga gcagcttttt tacaacctct tggtacacgg
    14941 tgttcaatca gctgaccctc ggtattagct caaaaaaacg gcaaaatatg aggaactatt
    15001 tttccatttt tttctctaat actcaggtca aaacactgat ttcacaacct ctttttttaa
    15061 tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg tcattttttc ttcgatgaac
    15121 ggatagattg ccgttcctac gattccaaag acaacaaaca aaccgatcat gatccagatt
    15181 gctttcgtag catttggcca ataaattcct cctgcagatt cccgtaacaa gtttaccgca
    15241 tgagtaaatg gcaagaacgg attgatcatt tggaagaatt tccctgagac ttggatcgga
    15301 tagttcccgc ctccgccaga gatcgacagt actaagatga taatagcgat ccctttaccg
    15361 acattatcga ataatgcagc caagacatag acgatcatca tgaacgctaa ggcgatcaag
    15421 acagcaaatg acacgctgta gaccggattt tggacatcta ctcctagtaa gaaaatattc
    15481 cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca aaaatgtcag catacgagca
    15541 acgaattgtt ctcgtttcgt aaaattgttt cggtctttct ttcctaaata gtattctgtc
    15601 gtagcaacac ttgaaagaag aacagcgccg acccacagac atagtgcagt ataaaacggg
    15661 gtgctagctg aaccgttatt tgcgattgga taaagcgcat tggtttgtag ctcaacaggt
    15721 tgtgtaaaga agtcactttc ttttgttgcg tctagtttca ataatttgat gacttcgcct
    15781 agatcgactt cttgttcgcc ttttcggatc gcgtctgctg ctttttggat accagctttg
    15841 atatttggcc agtcattgtt gatcaagtcc gcagcagcgt tcacagctgt ttcgacttct
    15901 ggcatttttt catttactgt tgtcaatgta ccggtgattt cattttttag tgctggccaa
    15961 tcattttgga taaaatcagc ggccaatcct aatttttgct gaatgatcgg taattcattg
    16021 ttgtataggt ctgccccttc attgatgcca ttgacgatgg tatccatatt tccattcagc
    16081 attgtattgg catcatggat ttcttgtcca attgcaggca tttctttttg atatttttct
    16141 aaaattgcca ctgcattcgt tacagttgct ttcgttgagc tgagaagcga atcaaagtcg
    16201 atctggtccg ctttgctcag tgcgccttgt gcagtcccaa tcgtatcgat gacttggtct
    16261 agtagagaag tgacagtgga actgatttga cctgtattga taccagcaat gatgccattg
    16321 atattattaa caacggcatc aatttgatct agcaattcat caatagcctt cgtgtcttca
    16381 ttttgaacag ctgtgttcaa agcagatagg cggttttgca gattgcttag caagggtttt
    16441 aattgattta gctgatcaat aattggctgt aatttcgtat ttccagcact tgtttgcaat
    16501 ttagtcagga actcgaccag tttatcgatt gcattttgtt gttgagatat tccgtcaata
    16561 aagccgttca atacattgct gatgtttttc ttttcatcat cagtcagctt accatcttca
    16621 atagctgttc ttacagtatc tattaacgaa cctgtcgttg ttgccacttg tccaattgac
    16681 ttcaatgtaa catctacact gtttgtgatg cttggtatcg catctttcag ctgagaggca
    16741 cctgattttg tttggtttgc tagatcactt gcctgatttt ctaaattttt cacatctggc
    16801 aagatatctt gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc gattccttgg
    16861 ttcattgtct cttggatcga agcaaaatcg ccgtcgatct ctgctagttg cttaccggca
    16921 ttttggatct ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg atctttcaac
    16981 tcaggcattt tgtcattcaa tgcaacgact ttttcaccca tctcatctac tttagggatg
    17041 taatccacga attcatttgc tttattcagt ttttccttga tttcaggtag ttgactttgt
    17101 aattcgagga cttgttttgt gtagccgtcg atcgtgtcca aattttcatc tgttgataaa
    17161 atcatatctt taactttatt gatacttact aggttcgtat cgatgtcgta acctatctca
    17221 ttgaatactt tcaataaagt cgaactggct gttttgataa attcatttgt gatttgcgat
    17281 tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat tttttcattt
    17341 accgtatatt cgatttttgg tttcttgata tcaccactag taaaactcag cagatcttct
    17401 gagaaatcct ttggcaagta aatacctgcg taatactttc cggaacggac tccgtcttct
    17461 aattgttctt ttgaatcaac gaattgccat cctaattgtt tatttttatg aagcgattcg
    17521 atgacttgct ccccaatggc cacttcttta ccttgaaact ctgctggctt gtctgcactg
    17581 taaacagcaa ttggcagctc tccagtattt ccatatggat cccataatgc tttgatgtta
    17641 aaccacgcat atagcgaagg gatgatcatg atcgcgatga tcagaagtgt tgcaatcggg
    17701 tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt gattgactgc
    17761 atagttctcc cccttcttct tttttcagct gaattacttt accatgtatt tcatactttt
    17821 ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat atcacggcaa ttgtctcatt
    17881 gtaatctctt acatattgag aaataattaa ggattacagc ctttacttta tcatgaaata
    17941 aaagggaaag ataattttat gccaaagttt ttatctatta tacgtatacg caaaaaagct
    18001 aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt ttttattcct
    18061 gtatttctta tttttcgaat gacaggatga ttttcccagc aggaggatgt ccttcaacta
    18121 gctgatgtgc ttggatgaca tgcttaagtg aagcagacag attcattgaa ataaaaacat
    18181 gaaaggctcc tttttggtag gctttgatta tgccagcaaa ggcttctgca tctagatatt
    18241 cctttcttgg aacaaacgat tcataaaagc cttccttttt ttgccgcaaa tctagatctg
    18301 ggaggtcatt taatgcaaca tacctgccgc ctggtttcat gatctggatg ccggtttctc
    18361 ctttgatact tcctttcgtc gcatcgatta caaggtctgc ctgatcagca aattgcagac
    18421 ctggatttgt tttatcataa gcagcaaatg ctgaagctcc taattttttt accttttcct
    18481 catttttact ggatgcactg gttaagattc ggattccctt ttcatggaga agctggatca
    18541 gacttgaccc gaccgcacct gatgctccta gtatcattac agtatctgtc ggctggattt
    18601 ccgtcaaatg gttgattaga ttataagccg taattcctgg cgtgaccatt ccggctgctt
    18661 cttcccaact cattttatct ggaatttttg ctacttttgc tgatggcaac acgacttctt
    18721 ctccgtaagt cccgccgact gcatggacag ctactcgatc acctacatga acagttgtga
    18781 catctgaacc aatctctgtc acaataccag cgccatcatt acctggtaca taagggaatt
    18841 tcaatgttcg tatctctttc atttctccta aacgcaaggc aacatcataa ggattcacac
    18901 tgaatgcttt tatttctaca cgaacatgtc cagaaccgac ttctctggga tgagcatcga
    18961 tttcttcaaa aacatcttgt gcttctccgt aaccgttaat tgcaaatctt cgcatggtca
    19021 ccatctccct ttttctttaa gtttacgttt atgcctttat tttgacaaaa aaaatgtctt
    19081 ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa cgtgttaaat
    19141 tatttatcta aaagcgtaaa gattacgatt tacgctaata aaaaataatt ggaggaattt
    19201 gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa cgattgaacg
    19261 ctatgctgct ttacgccagg aattaaaagc gcaaaaagat tacgaaggct tgcgtaaact
    19321 tcctttagat gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg gacgccctcg
    19381 cggatacatg cgtaaattcg ggatgtcccg tataaaattc cgtgaattag cacataaagg
    19441 tttgattcca ggagtaaaaa aagccagctg gtaatccaag tctggctgta atgtaaagga
    19501 ggagggcgca tggaccactc cttggatcat caaaaaagat acgttcgctt cttctagcag
    19561 aaatgctggt actagaagca gacgtatctt tttttacgca cagctggtcc ttttttatat
    19621 gtaaaggctg catggttact tcgctatatt tatttagtaa caggagtcag tgcttcgata
    19681 cccacactaa ttttcaatgc tcggtcaact tctgccataa tgggtcgatc caaatggcag
    19741 accttttctt tcaatcttga tttatcgatc gttctgatct gctccaacag gataacggag
    19801 tctctttcta ttcctgttcc agtagctttg atcccaatgt gagtcggaag cttaggttta
    19861 gccatttttg ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt acctagatta
    19921 ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg atcccacgac tggagaaaga
    19981 tcagcaaaat aaatatctcc tctttttacc ataggatcct ccttattctt gatattctct
    20041 tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta attgatctgc
    20101 tatatcctgc atagtgattt ctttgtctgc atttttgccg ataagcgtca cttttgttcc
    20161 gacaggaaat tcttgaggca atcggatcat cagttgatcc atacatacac gcccaatcgt
    20221 ttcacagtag ttcccttcta ccaatagaga aaaaccctgc atctttcgtg gccagccatc
    20281 tgcataaccg atcggtatcg ttccgatcca ctctgcttca ggagtgatat atgtttctcc
    20341 atagccgatt ccttcacctg ctgggagttt tttgacttga atcaattcag aaaccagctc
    20401 aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc ccagagggat taagtccgta
    20461 cattgctact ccataacgaa tcatattgcc gattgtttca tcatgccata aagccgttgc
    20521 actattgctg acatgtacat atcgaggaag ttcgggaagt tttttcagca cttctataaa
    20581 gcgttctttt tgcagattcc aatatgtatc atctgcctga tctgctgtag aaaaatgggt
    20641 aaataaacct tcccattcta atacacgact ttctttgatg aatgcagctg cctcttttac
    20701 ttcttcaggt gtacaaaatc cgatacgacc catcccagtg tctgctttta gatgaatcga
    20761 aagaggcgtc tctagcttac ttttctctaa aacagcagct gctgcttgca accattcccg
    20821 tgtacctgca gttactgata aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac
    20881 catattcaag ataaggatgg gctgagtaaa tccagcttct ctcaactcga ctccttcatc
    20941 taaatttgaa acacaaaagc ccgaagctcc acctgcaact gctgcctcag ctgtttcaat
    21001 agctccatga ccgtatccgt tcgcttttac tactgcaaac aattcttttc cttgcggaag
    21061 tcttgcaact tcatttgcta cattttcagt tatagctttt ttatgtatca ctgctttcgt
    21121 tgggcggtgc caagcgacaa ccatcgtttt tccttctttc tagtcttcca aaataatttg
    21181 tgcaaaagct gtttcatttg tatgagtaat actgacccat acattgcctt catgaggtga
    21241 acgcgtcaca acaggctgac cattttcatt ttttaaaatc tcgacatctt gaaaggtaac
    21301 ttttccgatc ccagttcccc aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc
    21361 taaaaattct acttggcgat gaaaaggcaa ggactggaat agtttcattt catcagaggt
    21421 caagattcgt gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc taggaagttc
    21481 gactgcatcg atcccaattc cttttatcat atgcttttct cttttcaata aatttagaac
    21541 actctcattc taccaaatta taaccatttt gccacaaata agtccacaaa taacaaaaat
    21601 gaaagtttct cttaaaaaag agaaactttc attttatgtt taatcgttat ttgaacggat
    21661 tacaaatccg cgttttttat cattgtgacg tttaccgcca tctttttggc tgttattatt
    21721 tttgctataa ccgccttttg atttgtttcc acgataatta ccgccgtcac gacgattacg
    21781 gttgtttccg ccaccacgtt tgccattttt attgtatcct tttttctgca ttggcaatgg
    21841 gcgttctggt gtgatcttca ctggcactgc atcagctgga tcttttgctg tcgtttttag
    21901 caaaagtgca actaaatctt gtgcagaata ttcttctaat aatttatctg ctgtttgcag
    21961 gtatttatct agaccatttt ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa
    22021 ttgacctttg aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt
    22081 caagttttca atcacatgca agtagtccat ttcatttggt gtaacgaaag tcacagacat
    22141 accgcctttt ccggcacggc ctgtacgtcc gatacggtga acgtagcttt ctggatcttg
    22201 agggatatca tagttgtaca catgagtgac acctgaaata tctaatccac gtgcagcaac
    22261 gtctgtcgct acaaggatat ctaaatgacc gctcttaaat gaacgcaata cgctcattcg
    22321 tttttgttga gacaaatcgc catggattcc ttctgcacgg taaccgcgtg cttctaatcc
    22381 gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag acgattgtta attcaggtgt
    22441 ttgaacatca aacaagcgag tcatgatatc gaatttttcg tattcttttg cgcggacata
    22501 gtattgatcg atcaagtcag ctgtcatttc tttcgctttg atcttcacat gatgtggatt
    22561 tttcatgaat ttaacgccaa tattcttgat agctggcggc atcgttgctg agaaaagtaa
    22621 tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg atatcttcca agaagcccat
    22681 attcaacatt tcatctgctt catccaaaac taatgtttga acggttccaa gtttcaatgt
    22741 gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat gtggacgatc
    22801 tttcaagcca cggatctgac ggccaatatc tgctccgccg taaacggctt gtacacgtac
    22861 ttttttgtcg cgccctaaac gatataattc ttcttgtgtt tggatagcta attcacgtgt
    22921 tggtgcgata actaaacctt gtaattcgtg acgatctgga tcgatttttt ctaacattgg
    22981 taatccaaat gcagctgttt tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc
    23041 agctaatgct agtggaatcg tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg
    23101 ttcgactgat ttcaacaatt ctggtgataa ttctagttct ttaaatttca aatgaatcct
    23161 cctatatggt tgttaactga ggatgtagct tttttgctac cccgtcgatt tgttcggtca
    23221 aagtgtattg tttctttttg acagtggcca cgcacgtttt tatccattct tagcccctgt
    23281 ggcaccaccg aaaaatcact ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt
    23341 tcagctttat cattattatg acatctggct tcaacaagtt gtttctatgg caaaaccagt
    23401 ccaatttctc ttcatctttc ggcaataggt tattctaaca tagtttttcg ttttctgcaa
    23461 gtttacagat ttttatttca tttgctgaag ttgttctacc acttcaaata agcccatgct
    23521 gttgctgcct tttagcataa tactatctgt tgcttcaaga ttttgtttga tagcatcaat
    23581 caatttattc ttttcttttt caaaatagaa cacaggtagt tgggggtatt tttggttcaa
    23641 ttcttctttt agtgcatgca tttgttcccc ataaaggaaa atcatggcga atttctcagg
    23701 atcgatatgt tcactcattt gcgcatgcat atttaaagac tctttcccta attccagcat
    23761 atctgctaac acagcgattc tgcgtccttt tgttggcaga tttttaaaag tatctaatac
    23821 cagtcccatg gctgttggat ttgcattgta gacatcgctc aaaatagctg caccatttcc
    23881 tgccgttagc cattcggttc tgttttgagt gatagaaaca tgggcgagcc cttggcgtat
    23941 ctcaggtatg gacagtccaa agaagcgccc gatcgtatac gcaatcaaag cattcgttac
    24001 attataactt ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa aatcggtttg
    24061 tgcttttcct tttgctgtca cttccgcatg aacatcgcca tttccaatac cgaatgttgt
    24121 gactgtttgt actaatgttg ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac
    24181 gagtaatccg tctgatacaa gtccgtcagt gatttccatt ttggctttag cgattccttc
    24241 tcgcgaccca agattttcta tgtgtgcttc accgatcatt gtgattgctg cagcttttgg
    24301 ttgagccata agtgaaagtt ccgtgatttc attggcgtga tccatgccca tttccaaaac
    24361 gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt
    24421 gtaatttcct tgcgtcttat atgttttgta tttttcagac aagacagacg cggttagatc
    24481 tttcgtggtg gttttaccat tgcttcctgt aattgcaacg acttctgcgc ctactttttc
    24541 caagtaatag gttgccaatt gctgcatggc tttcaaagga tctgctacag gaataactgg
    24601 aaaatttttt ggagcttgtt caacaggcaa gctccaaaat gaagcagatg ctccgtttgt
    24661 gatcgcttgc tggataaatt catgtccatc tcgcgtacca gcaagaggaa cgaataaact
    24721 tccttcggtt atcttccggc tgtcaaattc gacattggta atctttgtca gtgcagagca
    24781 ttcgctgccc acggcttgtg caatttcttg tattgttaga ttcattgaga tctctcctaa
    24841 aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc tcacttccaa
    24901 gtaaatttct ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa tcactccagc
    24961 ctcttcttta tttctcttta ttcatttttt tcaaaaaaag attgacgctg atggtatcga
    25021 ttcattccta actggatcag ttcttcaatc aaatcaccgt atttcaagcc catattttcc
    25081 cataagagtg ggtacatact gaactccgta aatcctggca tagagtttaa ttcattcagg
    25141 aataattcat ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct tccacctaac
    25201 atcgtgtaag ctaacttcgc gtactcttgc gctttttgat aaacttcttc tggcacttcg
    25261 gctggaatct gcatttcgat tttattattg atatattttg cttcataatc atagaatgct
    25321 acgtctttta cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc taatacagca
    25381 acttcgattt cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc atactgatag
    25441 gctgttgcta aagcattttg cagctcttct cggttttctg cttttgtaat gccgacacta
    25501 gaacccatat tcgccggttt gacaaacatc ggataaagca aagaaccttc acattgatca
    25561 aatacttttt taggattttc tttccattga ttcttaagta ctggtacata aggaacttgc
    25621 ggcacaccag cagcttgtaa aatatacttg gtcatgattt tatccatggc acatgcactg
    25681 gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt
    25741 ccatcttccc cgtttggccc atgtaaaact ggaaaaacga tggctccttc ttctttgatt
    25801 tcgcccggat tgatcacttt tcctgtaaag ccttcctctg tctgtccact tgggtcccat
    25861 gaaagatgca agacatcttt gctggcaggt ttttctgtta gtaatggacc tttgacccat
    25921 tgtccttctt ttgtaataaa tacgagttga acttggtaat aattataata aatggcattt
    25981 aaaactgaaa atgcggaaag aatggacact tcatgctctg cgctgcgtcc gccatatagt
    26041 aaagtaatct tcaaaagatt gtcctcctgt attctaaatc gttcagtgat ttactctcat
    26101 attttagcac aaaacaaaaa taaacgaata acttatttat aaaaaaacga gccagcttct
    26161 aaaagaacga gcgtcattta ttttttgtca gctagattat ttgtcccgtt ttttataaaa
    26221 agttatgata aagaaaagga gggatgatga tggcgcaaac aaatccacgc agccgcttgg
    26281 cagaaatcat tcgagtgttc atcaattaca atgtagtgcc aaactttgta cagcaaaaaa
    26341 atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc caccttcatc aagataggac
    26401 agatgctttc tgttcgagaa gatttgcttt cttctgcctt cactcaaaca ttcaaaacat
    26461 tacaagatag tgtacctagc gacacatttt caacggtcaa aaagacgatc gaaacagaac
    26521 tttctctttc attatcagat atattcgatg atttttcaaa atcgcctttt gcctctgctt
    26581 ctatggggca ggctcatcgt gccaagctga agaacggtga cgcggtagtc gttaagatcc
    26641 aacaccctaa catcgccgaa gagatccgac ttgatcttca actatttgaa cgagcgatcc
    26701 ctttgatcaa atacattcct gaaactagcg tagttgatct aaaaggggta ctccaagaag
    26761 taaaacgttc cttgatcaat gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat
    26821 tctatcaaaa gaacaatggc tggaaagaga tccgcagccc caagatttat gacgcttttt
    26881 gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat catttaatga
    26941 acgccgaaaa caaaactgaa acttttatca ctggaattca aaacaagcag ctgaaacaag
    27001 aagttgcaaa gcttttagtt gagaacttta tgaaacaggt gtttgatgat ggttttttcc
    27061 atgctgatcc tcatccaggg aatctgctat tccatgtact aacaaaagaa gaacagacac
    27121 aagctagccg aaaaactgaa acagtccatg aaaaagagtt cggttccttt gcttttcgag
    27181 cctccacttc tgcagaagac cctgttgcgc catataccat caactacatc gattttggta
    27241 tgatggggca tctttctgca ggacttaggc aaaaactcac acaagccgtt ttagcgctgt
    27301 atacgaaaga tgcttatcga atcgaaaaag cggtccttcg actttgtcag caagaaggct
    27361 cgtttgatga aagtcggttc caccaagaac tgacaagttt tcttgaacag tattatgatt
    27421 ctcctattga tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc
    27481 aaaataatct gcagttcgat cgtgatatca cgcttttgct gaaagcattt gggacattag
    27541 aaggggtgat ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct tcgccttttg
    27601 ctcaacatta ttttctcact catctggatg tagaagatac attgaaacaa agcggattag
    27661 acttattgga agggatgaag gctgcgccta agatccctca acagctccac catctattag
    27721 aaatgtggac ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa gacaaattat
    27781 tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg tatgatttta gctgcattga
    27841 tcgtagggtc ctccctgctt gtccaagcag cacctgtaga aaacgcagag gtcgtttcct
    27901 tattagggat atttacgtat gctattgccg cattcgtcat tatttttttg gcaatcgatg
    27961 cattgatcca aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca
    28021 gcttcaagaa ataagaaggg gttttacata aattgtttct caaatttttg tgaaaattcg
    28081 gcttattttt cgaagaggct acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt
    28141 tctgcaccca gtaataagaa caatcaaaaa gggaacatgg aacacattta tcctgtttcc
    28201 ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct actatcttct
    28261 gtacttggta aaccttagtc aattgccttt tttaacgctt gacgaaaaga catatgatct
    28321 ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa ctttccacta
    28381 tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt tccagagtga
    28441 tggaacactc gcacaccctt cggcaataca agtgaagcta aaccataatc ttgcgtgatc
    28501 aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc agctccgcgg
    28561 tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg gtccacactg
    28621 gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc ttcttttact
    28681 ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc ataaatgata
    28741 aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca agataagcag
    28801 ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata gatggattgg
    28861 ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact ggtggatcac
    28921 actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg gaatagctgt
    28981 atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc agaaactaag
    29041 cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag aagaactcag
    29101 tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc atggtccgac
    29161 tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg aaagtttttc
    29221 taatccgtac agtatgccaa cataaaaact gctaaataaa aaaaggaaat tccctagcat
    29281 tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct tcttctggtg
    29341 tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag caagatcaaa
    29401 aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc caaagaatat
    29461 caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt tgaaatccag
    29521 ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat
    29581 agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat acaggagaaa
    29641 tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac cttgcgtctt
    29701 ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca gccctagcaa
    29761 aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa gcaatgaacc
    29821 agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac caaatcgtag
    29881 actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa atggcaaaat
    29941 cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa taaaaataac
    30001 cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt
    30061 ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt cgattttagc
    30121 attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa atacagaaag
    30181 aagcataagc ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc
    30241 tccacaaaag tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt
    30301 atcctgtgcc tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg
    30361 cataaattta ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc
    30421 gaccatttga aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc
    30481 atgcttataa acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct
    30541 ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg
    30601 accacgtact ttatttagca aatataattt acttaaataa atttttccat ttatctcttc
    30661 ataagcagtg taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc
    30721 tgtcagttct ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg
    30781 tttttgacca atgatcggca caaaaacttc cggccatact tcttgtatga ctgccatcag
    30841 ctcatccagt tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca
    30901 ttataaatga ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac
    30961 cgaccacggt ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa
    31021 accagctact agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag
    31081 tactttttta atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt
    31141 tggcgataaa ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat
    31201 tgcttcccca atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat
    31261 cagcatgccc gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac
    31321 ttgcaacaga acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa
    31381 agacgtgctc gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa
    31441 aaacagacca ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga
    31501 catctatatt ccccacatct tttttcattt attaaaactg cctcctt
//
LOCUS       DBKOIJ010000015        30687 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000015 DBKOIJ010000000
VERSION     DBKOIJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.015"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(223..3876)
                     /gene="rpoC"
                     /locus_tag="ACV5NJ_000549"
     CDS             complement(223..3876)
                     /gene="rpoC"
                     /locus_tag="ACV5NJ_000549"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HHY6484672.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3955..7572)
                     /gene="rpoB"
                     /locus_tag="ACV5NJ_000550"
     CDS             complement(3955..7572)
                     /gene="rpoB"
                     /locus_tag="ACV5NJ_000550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HHY6484673.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8300..9268
                     /locus_tag="ACV5NJ_000551"
     CDS             8300..9268
                     /locus_tag="ACV5NJ_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6484674.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9286..10191
                     /locus_tag="ACV5NJ_000552"
     CDS             9286..10191
                     /locus_tag="ACV5NJ_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484675.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10188..10994
                     /locus_tag="ACV5NJ_000553"
     CDS             10188..10994
                     /locus_tag="ACV5NJ_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484676.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11151..12119
                     /locus_tag="ACV5NJ_000554"
     CDS             11151..12119
                     /locus_tag="ACV5NJ_000554"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HHY6484677.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12436..12960)
                     /locus_tag="ACV5NJ_000555"
     CDS             complement(12436..12960)
                     /locus_tag="ACV5NJ_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HHY6484678.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13247..14944
                     /gene="scm"
                     /locus_tag="ACV5NJ_000556"
     CDS             13247..14944
                     /gene="scm"
                     /locus_tag="ACV5NJ_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HHY6484679.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15099..16085)
                     /locus_tag="ACV5NJ_000557"
     CDS             complement(15099..16085)
                     /locus_tag="ACV5NJ_000557"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HHY6484680.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16108..16674)
                     /locus_tag="ACV5NJ_000558"
     CDS             complement(16108..16674)
                     /locus_tag="ACV5NJ_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HHY6484681.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQRTKQKWSPMFTKK"
     gene            complement(16688..17104)
                     /locus_tag="ACV5NJ_000559"
     CDS             complement(16688..17104)
                     /locus_tag="ACV5NJ_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HHY6484682.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17561..19495)
                     /locus_tag="ACV5NJ_000560"
     CDS             complement(17561..19495)
                     /locus_tag="ACV5NJ_000560"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHY6484683.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19797..20732
                     /gene="pplA"
                     /locus_tag="ACV5NJ_000561"
     CDS             19797..20732
                     /gene="pplA"
                     /locus_tag="ACV5NJ_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HHY6484684.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20822..21925
                     /locus_tag="ACV5NJ_000562"
     CDS             20822..21925
                     /locus_tag="ACV5NJ_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HHY6484685.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21938..22891
                     /gene="menA"
                     /locus_tag="ACV5NJ_000563"
     CDS             21938..22891
                     /gene="menA"
                     /locus_tag="ACV5NJ_000563"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HHY6484686.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23705..25051)
                     /gene="gor"
                     /locus_tag="ACV5NJ_000564"
     CDS             complement(23705..25051)
                     /gene="gor"
                     /locus_tag="ACV5NJ_000564"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HHY6484687.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25185..26438)
                     /locus_tag="ACV5NJ_000565"
     CDS             complement(25185..26438)
                     /locus_tag="ACV5NJ_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HHY6484688.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26444..27367)
                     /locus_tag="ACV5NJ_000566"
     CDS             complement(26444..27367)
                     /locus_tag="ACV5NJ_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HHY6484689.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27588..30080)
                     /locus_tag="ACV5NJ_000567"
     CDS             complement(27588..30080)
                     /locus_tag="ACV5NJ_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HHY6484690.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(30087..30554)
                     /locus_tag="ACV5NJ_000568"
     CDS             complement(30087..30554)
                     /locus_tag="ACV5NJ_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HHY6484691.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         8872 a         6519 c         5727 g         9569 t
ORIGIN      
        1 cgctctatct tcccatcaat ttattttgca ttcaaaaaat gatctatctt tacttttgtt
       61 gattgacttc tcttggaaga tgatcgttcc gataccttaa ttggatacga accgatgttg
      121 ttaagatcct ttcccacttt tcttctagtt agacaaatta ttttagacaa accaaaaaag
      181 aggttgtgac aataatttgt cacaacctct tttttaaaat atctattttg tttgattgtt
      241 cattgcgtct tctgcagcca tttgcgcttc aatatctgaa atactgtaga cattttcgct
      301 tgctacaccg acttcttttg gttccatgtc acgataacgt tgcataccag taccagctgg
      361 gatgatctta cctataataa cattttcttt caaaccaaga agaggatctt tcttaccgcg
      421 gatcgcagca tccgtcaaga cacgagttgt ttcttggaat gatgcagcag ataagaaact
      481 atttgtttct aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac
      541 gcctcctgag actagcgtat tgtagttttg atctttaaag tctgcgatgt ctaataatgt
      601 acctggtaag atatctgtat cacctggatc cattacacga actttacgca gcatttgacg
      661 aaccattact tcgatatgct tgtcgccgat ttctacccct tgcatacggt aaacacgttg
      721 tacttcacga agcaagtagt tttcaacaga taatacatcg cgaacttgca acaattgttt
      781 tggatcgatt gatccttctg tcaatggtgc accacggtgg atcatatcgc cttctgctac
      841 tttcatacgt gctgtataag gaacagtata cgtacgtgta tctgtttttc ctttgattgt
      901 tacttctttg gtacgtgttg caggatcttc tgagatatcg atgacatccc ctgttacttc
      961 tgtgatcact gcttgccctt tcggattacg tgcttcaaag atttcttgga cacgaggaag
     1021 accttgagtg atatcgtctc cggcaacccc acctgtatgg aatgtacgca tcgtcaactg
     1081 agtaccaggt tcaccgattg attgagcggc gatcgtacca actgcttctc cgacttcaac
     1141 gtcagagcca gttgcaaggt tgcgtccgta acaatgtttg cagacaccat gttttgtatt
     1201 acatgtgaag acagaacgga ttgtcactgt ttcgacacct gcatcaacga tttgttttgc
     1261 gatgtcttcg gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt caggatgacg
     1321 tacttcttta cgggtgtaac gtcccactaa acgatcttct aatgattcga tgatttcatt
     1381 tccctcgcgg atggcttgga tttcaagacc tcggtcagta ccgcaatctt cttcacggat
     1441 gattacatct tgggccacgt caaccagacg acgagtcaag taacctgaat cggcagtctt
     1501 caaggctgta tcggtcatcc ctttacgagc cccgtgagtt gagataaaca tttccaagac
     1561 ggatagtcct tcacggaagt tcgaaatgat tggcaattcc atgattcgac cattaggagc
     1621 ggccatcaaa ccacgcattc cggcaagctg tgtaaagttg gaaatgttac cacgagctcc
     1681 agaatcagac atcatgaaga ttggattttt cgcttccaaa ccttccatca gtttttgttg
     1741 gatgctgtct tttgcagcat tccaaacagc aatgacacgt tcataacgtt cgtcatctgt
     1801 gatcagacca cgacggaatt gttttgtaat agtttcaact tgtttatggg cattttcaat
     1861 gatttcttgt ttttcattca gtacactgat gtcagcgatc cctactgtga taccggcatg
     1921 tgtcgaatgt ttgtacccta agtctttcat acggtcaagc atcttagaag tttctgttac
     1981 tttgaatcgt ttgaagactt ctgcgatgat gtttcctaag tttttcttct tgaacggtaa
     2041 tactaattct tgctctttga tatgtgcagg aatatcagta ccagcttcaa caaagtattt
     2101 gtccggtgtt tggacggtca agttgaagtc tgttggttca ttcagataag ggaattcagg
     2161 aggcatgatc tcgttgaaga tgatcttacc aactgttgtg atcatcgtac gttctttttg
     2221 ccattcagta aatggttttt cacctaatga gttagggttc acaccgatac gtgaatgtaa
     2281 atgaacatat ccgttgcgcc atgcgataac ggcttcgttt tgatctctga agaccattcc
     2341 ttcgccttcg cgtccttctt cttccattgt caagtagtag ttacctagaa ccatatcttg
     2401 agaaggagta acaactggtt taccatcttt agggttcaag atgttttgag cagctaacat
     2461 caacatacgt gcttctgctt gtgcttcttc attcaatgga acgtggacag ccatttggtc
     2521 tccatcgaaa tcggcattat aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc
     2581 ttgtactaaa actggttcga atgcttggat accaagtcgg tgaagcgtag gtgcccggtt
     2641 cagcaatact gggtgttcct tgattacgtc ttctaaaaca tcccatactt cgtcttcttg
     2701 acgttcgatt ttacgtttcg catttttgat gtttgaagct agttcgcgtt gaacaagctc
     2761 acgcatcacg aatggtttga ataattcaat ggccatttct tttggaagtc cacattggta
     2821 catcttcaag aaaggtccaa caacgataac ggaacgacct gagtagtcga cacgtttacc
     2881 tagcaagttt tgacggaaac gaccttgttt ccctttcaac atatgagaaa gagatttcaa
     2941 tgggcggtta cctggtccag taactggacg tccgcgacga ccgttatcga tcaatgcatc
     3001 tactgcttct tgcagcatac gtttttcatt ttgaacgatg attccaggtg cattcaagtc
     3061 taacaaacgt ttcaaacggt tattacggtt gattacacga cggtaaagat cattcaagtc
     3121 acttgttgca aaacgtccac cttctaattg aaccattggg cgtaaatctg gtggaataac
     3181 agggataacg tccataacca tccagcttgg ttgatttcct gaagcgcgga atgcttctaa
     3241 aatatccaaa cggcggattg cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc
     3301 tttcaattcg gctgcttctg catctagatc aacattatca agtaattgtt taatcgcttc
     3361 tgcacccata gcagcttgga attcttgtcc atattgttca cgtttttcgc gatattcgcg
     3421 ttctgtcaac aattgttttt tctctaaatt cgtatttcct ggttcgatga ctacatatga
     3481 agcaaaataa atgatttctt ccaaagcgcg tgggctcatg tctaatacaa gacccatacg
     3541 tgaaggaatc cctttaaagt accagatatg tgaaacagga gctgctaatt caatatgtcc
     3601 catgcgttca cggcgaactt tcgaacgagt aacttccact ccgcatcgat cacaaacgat
     3661 tcctttataa cggatacgtt tgtattttcc gcaggcacat tcccagtctt ttgtaggacc
     3721 aaaaatccgc tcacagaaca acccttcgcg ttcaggtttt aaggtacgat agttaatcgt
     3781 ctcaggtttt tttacttcac cgtaagacca gcttctgatt ttttcgggag aagccaaacc
     3841 tatttgcata ctttcgaatt tatttacatc gatcaaaagg ggttccctcc atttcattta
     3901 atggcaaacc gcaagacggc tgatcttcga ccgccttgca gctcatagac cggattagtc
     3961 taaattatct tctgcggttt caaagttttg gatgacatcg tctttttcat cttccacttg
     4021 ttcagcagct ttcttttcta attctttggc agtttgctgt tcggcaaatt ttgtcaaagc
     4081 atcaacagtg atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc
     4141 ttcgatatcc aagacacgca tgtccagacc tagtgattgc aattctttca ccaatacgcg
     4201 gaatgattcc ggtacacctg gttttggaat tggttcaccc ttgacgattg cttcgtatgt
     4261 tttcacacgt ccgactacgt catctgattt gtatgtcaag atttcttgta aagtataagc
     4321 cgcaccgtaa gcttccagtg cccatacttc catttcacca aaacgttgtc caccaaattg
     4381 agctttacct cccaatggtt gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg
     4441 caatttgtca tcgaccatgt gagcaagttt gatcatgtac atcacaccga cagacacacg
     4501 tccatcaaat ggttcacctg tacgtccgtc gtacagaaca gttttcgcat cgctggccat
     4561 accggcttca cgaactgttt cccatacgtc ttcatcactt gcaccatcaa atactggcgt
     4621 tgctacgtgg atacctaact gacgtgcagc catacccaag tgtaattcca atacttgccc
     4681 gatgttcata cgtgaaggca cccctaaagg attcagcatg atatcgattg gtgttccatc
     4741 aggtaggaat ggcatatctt cttccggcat aatacgggat acaacccctt tatttccgtg
     4801 acgaccggcc attttatccc cttcatggat tttacgtttt tgaacgatat aaacacgaac
     4861 taacatgttt acgcctggtg acaattcatc tccagcttca cgagtaaaga ttttcacatc
     4921 gtgaacgata ccgccgccgc catgtggtac acgtaatgat gtatcgcgga cttcacgagc
     4981 tttttcgcca aagatcgcat gcaatagacg ttcttctgca gataattctg ttaccccttt
     5041 tggcgtgact ttccctacaa gcaaatcgcc atcttggact tctgcaccaa tacggatgat
     5101 acccatttcg tctaaatctt ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat
     5161 ttcttcaggt cctaattttg tatcgcgggc ttctgattca tattcttcaa tatggataga
     5221 agtgtatacg tcatctttta ccaaacgacg gctcatgatg atcgcatctt catagttgta
     5281 cccttcccaa gtcatgaaac caactaaaac gttttggcct agagccattt cgccttgttc
     5341 catagatggt ccgtctgcca gagtatcccc tttttcgact ttctcaccca aatgaacgat
     5401 tggacgttgg ttgtagcttg ttccggagtt tgaacgacgg aattttgtta catcgtattt
     5461 atctaatgca ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac
     5521 accgtcatgt ttacataaca aagcagctcc tgagtcatgc gctgatttgt attccatacc
     5581 agtaccaacc catggtgctt gaggattgat caacggcact gcttgacgct gcatgttggc
     5641 acccatcaag gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac
     5701 ggcaactact tgtttaggtg atacgtccat gtagtctact ttatcaatag atacttctaa
     5761 gttttcactt tgagcacgag ccataacaac gtcttgagca aatgttccat cttcattcaa
     5821 tgggctgttt gcttgggcta cgatgtaatg gtcttcgata tcagctgtca agtagtcgat
     5881 ttggtcagta acacgacctg tttcacggtc tacacgacga taaggtgttt cgataaatcc
     5941 gaatttattt actttcgcat aactggacaa actattgatc aacccgatgt ttgggccttc
     6001 aggagtttcg atcggacaca tacggccata gtgggagtag tgaacgtcac gaacttcata
     6061 accggcgcgg tcacgagtca atccgccagg tcctaaggct gaaagacgac gtttgtgtgt
     6121 caattcgcca agagggtttg tttgatccat gaactgtgac aactgagaag aaccaaagaa
     6181 ttctttgata cttgccacaa ctggacggat attgatcaat tgttgaggtg tcaatgtttc
     6241 agtatcttgg atcgacattc tttcacgaac cacacgttcc atacgtgcta gcccgatacg
     6301 gaattggttt tgtaaaagtt caccgactga acggatacga cgatttccta agtggtcgat
     6361 atcatcgaca ttgccgatac cttccatcaa gttcaagaaa tagctcattg aagcaatgat
     6421 gtcggctgga cgaaccgttt ttacagctgc ttctggatag ccgtttccga tcacgtttac
     6481 ttcacgttca gggtctcttg gtgaaaatac tttgatgact tggacagtca ttggatctgt
     6541 gaccacaccg tcttctgatg gatagtaagt cactgaattt aggccattat caatgaaagg
     6601 agctagtgtt tccatgactt ggtgagtcag aactgttcct ttttcaacga tgatctcgcc
     6661 tgtttctgga tcaactaatg tttcagctaa tgtcagattc aatagacgtg ttttcaaatc
     6721 taattttttg tttactttgt aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa
     6781 gaaacgagca ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa
     6841 acgttcgtaa acgtctttca aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa
     6901 gtctttttcg actgtattgc gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc
     6961 atcggaacca aatcctaacg cacgaactaa tacggttaaa ggaatcttac gtgtacggtc
     7021 gatacgtaca taagagatgt cttttgcatc agtttccatt tctagccaag ctccacggtt
     7081 agggataaca gtagaaccga atccttcttt tccgtttttg tcgacttttc catggaaata
     7141 gacacctggt gaacggacta actgagaaac gattactcgc tctgccccgt tgatgatgaa
     7201 tgttccttgt tctgtcatta atgggaaatc accgaagaat acttcttgtg ctttgatttc
     7261 gccagtctcg cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc
     7321 atgcgcacga gcttctgcta ctgtatactt tggttctttc aattcataat ctacaaattc
     7381 caaagataga ttgccattga agtcatcgat tggcaaaatg tcttcaaaca tttctctaag
     7441 tccctcatct aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc
     7501 caacacttca ctgattcttg cgaaacttct acgttcgcga tgttttccgt attttactac
     7561 gtgtccagcc aagctcttca cccctctaaa atgtcattca aaaagactac ccgatcaatt
     7621 gttacaataa tatttcaaat attattttta ggtaacaaca gacatttttt gcggctttta
     7681 ggcaaaaaaa aaccaaaaat agaacattct cttgaaacct tacttcgtga gcttctactt
     7741 ttgtttcctt atattaaagc cgaaacggac aatatttcgt ttctgccttt tcttcatcag
     7801 atagtttttg caacttcata tactacactt attttctata aaagtcaaca cttcctggcg
     7861 tttcttctgc tttttcgcaa cattttctta atgttttcaa ttttatgttt tcaattatct
     7921 gacaattctt aattgacaga cctttttatc tcggttatac tttagaaaat tcattttgtg
     7981 gaggaacttc tatgcgaacc aacatcaacc aaatcgcacc aacacaactg aataactttt
     8041 actttagcta ctttagatag gtttgtattc atcatgatgg atacaaacgt taaacgacag
     8101 tttgtatcta tgatggctaa agaactttgt attgcagcaa aaagccacgt agacaaaatg
     8161 ctaagtggct gagcagtgaa aatacaaagt aaaagtatca agcattctac tttttcaatt
     8221 tattttccga cttttcaatc catttggaca ttttgtctga atggattttt tgtttcccaa
     8281 aattaaggag gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct
     8341 actagcttta ggaggttgcg cacaaaaaga caattccgca gaggcatcag atggacaaaa
     8401 aacaattggc gtattgcaac ttgtacaaca taactcgtta gatgctgcct ataaaggatt
     8461 caaagaagga ttagcagaag aagggtataa agaaggcgat aatctaacaa tcgattatca
     8521 aaatgcccaa aacaatcaag acaacctgaa aagtatgagt gagaaactcg tcaaagaaga
     8581 tcctgatttg ctacttggaa tcgccactcc agcagcacaa tctttattaa atgaaacaac
     8641 cactatcccg atgactgtca cagcggtcac agacttggaa caagcccatt tagtcacatc
     8701 caatgaaaaa cctggcggca atgtgactgg cacgacagat atggtgccaa tcgaaaaaca
     8761 aatcggctta ctgttaaata tcgtgccaga cgccaaaaca atcggcatca tgtataacaa
     8821 cggtgaagca aactctaaga tccaagctga tttggcagaa aaagaattga aaaaagcggg
     8881 cgtcaaagtc aaagtactga cagcaaattc cacaaatgat gtccaacaag tgacgaaaag
     8941 tctagctaaa gatgtagatg gaatctatat tcctactgat aacacgttcg caagtgcggc
     9001 aagtgttgtc ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg gcgcagttga
     9061 tcaagtcaaa gacggcggtc tagcaacagt aggaatcgac tacgaaagct tgggcaaaca
     9121 aacagggatc atggcagcaa aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga
     9181 aacagcgaat gatttggaac tgtatgtcaa taaagacatg gccaaagcat taggcatcaa
     9241 tccagacagc atcaaagaag ctgaataaaa cacaggagga ttctcatgac aaatttgctc
     9301 tctcttttct tatcatctgc atcgcaaggg gtcttatggg ccctacttgc gattggtgta
     9361 tacgtcactt tccgtatctt ggacattgcc gacttgactg ctgaaggaag ctttccttta
     9421 ggtgcaggga tcgcggcagt aagtattaca aatggctatc atccattgac agcttgtctt
     9481 ctaggctttt tagggggtgc ggcagcaggg cttgtttccg gactgcttca cacaaaattg
     9541 aaaattcccg cccttcttgc agggatcatc accatgactg gtttatattc tgtcacatcc
     9601 cgtgtgatgg gtgcgccgaa catctcttta ttgggtcagc caacgatctt tcattgggtc
     9661 gagtcattcg gcataacaaa agacaacgct gtactgatcg tcggtctgct ttttgctttg
     9721 atcgttgtat gtttacttgt tttgttctta cgtacagaaa caggacttgc tattcgtgca
     9781 actggcgata atctggcaat gagcgaagca aacgggatca acacagataa tatgaagatc
     9841 atcggttata tgatttctaa tggatgcatt gctctttctg gctccttact tgcgcaaaac
     9901 aacggctttg ctgatttaaa ttctggtatt ggaacgatcg taattggact tgcttctatt
     9961 atcatcgcag aagttttgtt acgtaaccag ccactatttc tccgtctctt gactgtcgtt
    10021 ttaggtgcgg tcatttatcg ttttatttta gcgttagtct tcgaactgaa tgttgaacca
    10081 agtgattcga aacttgcttc tgctttcgtg ctagtgatct gtctttcttt cccgcagatc
    10141 cagcaaaaat tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct
    10201 taacattgga aaatctccat catacctttg aaaaaggaac gatcaacgaa aatcatgtcc
    10261 tgaaaggcat taatctttcc ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg
    10321 gggctggtaa atctactttg ctgaacagca tcgccggtac attttctgtc gatcagggtc
    10381 aaattttatt gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca
    10441 tcagtcgcgt attccaagat cctaaactag ggacagccgt tcgtttaacg gtagaagaaa
    10501 atcttgctct tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac
    10561 aagaccgaac atttttcaaa gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt
    10621 tatcctcaga aatcggtttg ctgtctggag gacaacgtca agctattacg ttattgatgg
    10681 ccacattaca gcgtccagaa ctgatcttat tagatgaaca cactgcagcg cttgaccctg
    10741 ctacttccaa aacggtgatg gaattgacag aaaagctgat cactgagcag aatctcactg
    10801 cttttatggt cactcatgac atggaagacg cgatccgcta tgggagtcga ctgatcatgc
    10861 tgcatcaagg aaggatcgtc gtagatatcc agcaagagga aaaacagcag ctcacagtgc
    10921 cccaattatt agcgttgttc aaacaaaaca gcggcacggc attaaaagac gatcaagtac
    10981 ttttatcttc ataatcagaa agaggatggg acaaaaaatg aacgcccacc ctcttctttt
    11041 ttctctttcc ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc gaaaactatt
    11101 ttgttctctc cttcttcctg atagaatgga tgggaaagga gcttgatgcc atgacaacga
    11161 aagacaaggt acttcaatta ttgaaaaaga caccagattt tctttccgga gaaaagttgg
    11221 cacagcaact ggaagtttcc cggacaagta tttggaaagc catcaaagaa ttggaaaagg
    11281 caggctatcg ttttgagcac gggcctacag ggtatcgtta cctgccctct gacgtactag
    11341 atgcaaaaga aatccagcaa gagctacggt cgtttattcc tgatttgaaa gtacaaacga
    11401 tgttgacttc tgaatctacg atgaaagatg ccaaactggc agcagctcaa ggagattctg
    11461 tttcgaagtt atttgttgct gatacacaag aacaagctcg tggacgtttt ggacggcctt
    11521 ttttcgctga acctggacga ggaatctaca tgagtctctt gctccatcca gacaaacggt
    11581 ttgatgaatt gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat
    11641 ctgtaaccgg tcgttctgct tcgatcaaat gggtcaatga tatctacatg gatggaaaaa
    11701 aagtctgtgg gattttatct gaagcagtca gtgatatgga aagcggcagg atcagtcata
    11761 tcatcattgg gatcgggctt aatttttctg ttcctcaaaa tcattttcca gctgatctcc
    11821 aagaaaaagc catctctgtc tttcccgacg gacaagctag tgtcacaagg aatcagctaa
    11881 tcagcaaaat ctggcaagaa ttgttcactt atctttctca cccagaaaaa gcgttggact
    11941 tctataaaga aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga
    12001 cttatgtcgg tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc caaacaaatg
    12061 aacagaagat gagtttgtct tctggcgaaa tcagtctcac atctatccgt acgcaataat
    12121 tatcagattt cacaaaagga cacctgatct catacagtgt ccttttttct gaattctctt
    12181 caaaagactc tccttattct atcagctgac agtttctcta tcctctagaa aaaggtattc
    12241 taaaaatatc tataaagcat ggtacacttt ctttaaatga atcaatgcaa aaaacaagag
    12301 agtcatcagt ttacacgatc aaaagagata gcaggccgct tcctttttca atcttataga
    12361 tagaccatgg gtaacgataa aaacaaaaaa gaacttgcga cttattttgc cgtaagttct
    12421 tttttagcta gtgccttaat ctcttaatcc caacaaacgt ctcgcataga tcacctgacc
    12481 agagtgcata gcagcatcat caataataga aacgagacgc attcctcgtg tgactggtgg
    12541 tgtccagttc tcgtcaatta cttcatctag ctcggaaggt tccagccttc ttaagtaaga
    12601 aatagcaaaa tcagcagctc ctcgcaagta tcctagcaga tcttctattt gattagcttg
    12661 gatctgcttt gcttgttcca gactatgctt ccaatcttgt tcgttatctg ctatcgtcat
    12721 cggaaaccgg ctttcccagt cttgactatg ccaaattgga gaagttcctg ctaaatcagc
    12781 aatctggaaa tctaattctc gagcggtatg ccaagccagc caagtcattg atttgatctg
    12841 cggggctcta tcttccactg gaaaacgatt ggcctcctca atagagacgc cttcaaacaa
    12901 acgttcaaac cgatcgtatg cccgatccac actctcgcat agcatatcta aataatccat
    12961 ttattttcct cctttttatt tatcctactt actttttcct aattctagga tacctactta
    13021 tatgttacta cacaccctta ttttttacac tttaacagtc accatcatca atagtttgga
    13081 gattgaaaga aaaagatagt gtcgaactat caattgaaca agaagcaaca atatgaacgt
    13141 ttcattaata ttctttaaat aaacaatgat ttttcaaacg aacagtttaa gactaggaga
    13201 aaaagagtat aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc
    13261 aagtttacta gtcctagttg ctttgttgtt caatatcttt tcgtatggtg tggccgtttc
    13321 agcggaaaca tacattccta aagaccctgg gaatatttat aatgatctaa ctggtaacta
    13381 tggcttgtta ggaattgcta gtcaattcca cgtatttgct aaaaataaga caactgtgaa
    13441 tgcacataca gacggaaata tagcaacaca tgaacttgat gcaaaaaata attttggtac
    13501 agagcttcat gaaggtttat tgaaccaaga aataaattat atacaaaaaa ctgattcttt
    13561 cgctaattct tcaggcatac ctacaggtag cacaagaatg aataaatttg tcgttggcag
    13621 taacgtggat gtagcacttc aagatggtca tcctacagtc aatggatcca ggcttgatca
    13681 cttaactcca gatgattttt ataaagatcg tgctgggcat gaatatattg atttcgagca
    13741 ggaatttgct aaattgaatg tagcagcaaa tgatcttgca acgatcactc cagctaaaac
    13801 atatactgct gcagattttc cagacatgaa caatcggaca atcgatttaa ctgatttatc
    13861 agataccggg tttttattgg taaatatcga tgcagaagtg ttaacgatga atacgccact
    13921 tcaaatcatt aatccaaatg atcaagtagt tgtatttaat gtcatcaaca gttcttctgc
    13981 attgaacgta cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa cagaagattt
    14041 ttcggatgcg aatatctctt ggaattttgg aaatgagatg actgatttga caatcagtgc
    14101 tccttttcaa ggaacaatcc ttgcgcctaa tgctactatc cgcattactc aaaaccaaga
    14161 cggaacgatt ataggaacaa atgtgatttt agatgcagct accaatcgtt gggatcctaa
    14221 cgaaatattt atcagcgaca atggtacaga tacgacagac acaagcgaca gtacagatac
    14281 atctaccaca agtgatagct cagattcatc aacttcaagc gacagtacag atactacctc
    14341 taccacaagt gatagcacgg atacatcagc ttcaagcgac agtacagata ctacctctac
    14401 cacaagtgat agcacggaca catcagcttc aagtgacagt acagatacta cctctaccac
    14461 aagtgatagc acggacacgt cagcttcaag cgacagtacg gatacatcta ccacaacaag
    14521 tgatagcacg gacacgtcag cttcaagtga cagtacagat actacctcta ccacaagtga
    14581 tagctcagat tcatctacca ctacaagtga tagcacggac acgtcagctt caagtgacag
    14641 tacagataca tcctctacca caagtgatag ctcagattca tcaacttcaa gcgatagtac
    14701 agacacgtcg tctaccacaa gcgatacacg tcatgcaagt gtctccttct caaaaacaag
    14761 ttcggataac aggacgaact ttaacttaac taataaaaca agcaatgaca atggaaacaa
    14821 atcaaaaaga gcacttccta aaactggaag tcaatcgaat aattggataa cactcgcagg
    14881 cgttatatta cttgtaatcg gtttaagaat cacttttatg agttataaga aatcgaaaag
    14941 gtaaaaaaat attcaagata aaaaagagca acgtcgctaa cggacgttgc tctttatttt
    15001 tgctttttat cataaataca agaactacaa ttcaatttgt caaaaaacac gtagatttcc
    15061 ttctgagaaa tttatttacg tatcgaacct ttccctattc agtctttgcg atccaaaatc
    15121 agttgggtaa gggaaaagag attctctttt gttcgaagag aggtggcagg taatttcttt
    15181 atttccttca aagccttttc tgtgtaagag gccgctaact tcctagcttc ttccacgcct
    15241 cctgtatgtt caacgatttt gcgtacggcg tcgacatctt cttctgtcat tgccgctttc
    15301 ttctctaaat aaggtagtag ctttggtttg tttttttgca aagagtagat caacggtaaa
    15361 gaatagaccc cttgacgcat gtcttctagt acaggtttgc cgatttcctg gctctctttt
    15421 gtataatcta aaatatcatc gataatctga aaggccatcc cgatagaaag tccgatatct
    15481 cctgcttttt ttgcgaagcg ctcgcttgtt ccactttcaa aagcgccaac agaacaactt
    15541 aattggaaca gttcagctgt cttacctgaa atattttcca aatattcttt tacagaaagt
    15601 tcataattat agcgatcatc catctgccct aattcgccat tcaatacttt ttccatactg
    15661 cgagaattca attggagact tttcaaggaa gtggagtaat cagacaatag tttaaaacag
    15721 cagacaaata aatagtctcc tgcatagaca gaaacgacat taccaaactg actacggata
    15781 ttaggctgac cacgtcgcag atccgcttca tcaacgatat catcatggat cagtgtggcc
    15841 gtatggagca tttcgataga agctgccaaa gcgatggctt tgtctcgatc ttgctctggt
    15901 ccaaattgtg agaacaatag ctgataggca ggacgcaaca gctttccgcc ggcatggatc
    15961 gtttctttga tggcttgttc gacaggtttg tttttcaggc ggacagtttc ttccatccgt
    16021 tttagtgttt ctgtcaattc tttacttaaa gcaggatagt tttcccacat tggatgtagc
    16081 ttcataaaaa aactccttgt gtgacgatta ttttttggta aacattgggc tccatttttg
    16141 tttggttctc tgctcataat ctttttggaa tcgttgaagt gtctgcacat gtcgtaaaag
    16201 ataattcgct gcgatcccga ttgcaatccc tgataaaatc cctaaaactg aaaggactgg
    16261 caagtacaac atgactgtcc atgattgtgc aatgaaagaa gccatcaaaa gctgtccgac
    16321 attatgcatg aaaccgccgg ccgcactaat tccgatgata ctgacacgtt ttggtcctaa
    16381 ttgcttgacc aataacattc caaaataact aagcaaagca ccactcatac tataaaggaa
    16441 tgtggaaatc gttccgccta gcaaagtagt caggaatagt cgtagccaaa gtagtaagaa
    16501 gctgtcttgt tttttcatgg taaaaatggc aacgatcgtg attagattgg ctaagcctag
    16561 ttttgcacca ggcgcaaaag aaaaaggata agggatcata ttttccaata aaccaatgat
    16621 cactccttgt gcaacaagta gcgaaatata aatcattttt tgtaatttac tcatggtgtg
    16681 tgaaggtcta ataaatcaga ctgtcttcgt cacctccatc tgttgaacgg acttcgatca
    16741 ctaatttatg tggcaaacag acgatggttt cgccattttt cgttgcccag cctcgacgta
    16801 cgcagatctg gtcatcgcaa tctgcttctt ttatccgtac tcgatcgcca tctatctcga
    16861 tcaagttata atctccgtgt tcatctttgt aagtatatgt gttgctctct tctccagcca
    16921 tcaaagggaa tgttttgatt tctgtgccat ccacacgcag taccgcttct ttttctgctg
    16981 ttgcggtgtt ttgctgaaga ccaaaaatca tgactggtaa aaaagaaaga acagtcaaaa
    17041 gcaaaataat aaaaacatcc caaggtttga gcagactttt ttcagcaaat tctttaaggc
    17101 tcatttttgt tctccttttt ccagattaac tcacccaatt ttagcataaa aacaagaggt
    17161 tgtgacacct gtgtcttgct ttgagtaata agaacgaaga atagaaaaca gctttccatg
    17221 ttttctaatt ctttgggctt attaccgaga agcagcaggt ggaacaccgt ttataacaag
    17281 aggttgtgac agaagtgggc aacttcaaga aataagaagg aatttacgaa aattactttt
    17341 caagtttttg tgaaatttcg gcttattttt cgaagaagct acttctggaa caccgtttat
    17401 aacaagaggt tgtaacagag gttgtgacac ctgtgtcttg ctcatggagg cttatctcgg
    17461 gcatcttatt cctttaattt tacataaaaa ggaggctgtg acggtttgtc tcagcccctt
    17521 tctattgaaa tcttgcattc aaataacgtt ctttatctat ttatttcttg ccgtactggg
    17581 aacttggtct accgtaccac caattaccta gtgtacgttg tagccatgga attacccagc
    17641 gatccaaccc aatcgcacgt ccggaaccgt tcatcaatgc gaaagcaaca aaaatgaacc
    17701 aaatgtttac ccagtaaaac atgcctgata agcagaacgt gatcaccaat acaatcgttg
    17761 tcgcactgca tagccatgtg aataatccag caatcaatgc taatgccaag gcaatttcaa
    17821 agaataccat gaatttttgg aagaacattg ctacatcatt gtttggcatg atcacttgca
    17881 tgatctttgt gaaccagtct ggtgacttct caatcacagc cattggttct tcaccgtatg
    17941 cataactcaa accaaaatgt gcagcttgag tcgctgcttc tccggcactt gcccctgctc
    18001 cgctggcagc tgtggttgca tcaccagcag cagaagcacc actggcaaca tctgttacat
    18061 tgtattccca tgtccaaggg aacacactgt ttccagtgaa ccatgatcct tcaccaaacc
    18121 aagtacttgg tttgaacact tcaccattcc caatgatttt tttggaagct tcaacgaacc
    18181 aaactaaacc atagaaaata cgaagtggca cactccataa gacattgccg taacgagaag
    18241 aatgtcctcg agtcacgttg cgatcatctt tgatatggaa aaactcatgc attaaatatt
    18301 ggaacatata ataaccagaa cgaatatcaa agaagtattt caaattcacg atatgtttca
    18361 ttacgatagc taaaaatccg cttaaatgga tcttatcaaa taggttagct accccccatt
    18421 tggcaccaat agaaaccata aatccttgat agtttccttt aaatttatgc ttttctccgc
    18481 ctttgatcga agccacgata ttcgctgcgg ctgtgtgtcc tgtttgttca gcagcttgaa
    18541 cgatctgcgg tgtcggtgtt cctgggaatt cttcataaaa gactaaatca ccaatgatat
    18601 agatgttttt atcttcgtag ccttttgcct gcatatattc attggcgatc aaccgctgtc
    18661 cgcgtgctga ttctaatcca aagtcagcag cgtcagaagt tcctttgaca ccagctgtcc
    18721 aaatcagtgt gtgagtcgga atgttgcgtc cgtctttcaa tttgatatga tcagcagcca
    18781 cttctacgat tggtgcattc aataataatt ccacgttgtg cttacgcaaa tagcgttcag
    18841 cttttgcagc atcatttcga tccaacatat tcaagatcgt cggcattgcc tctacgacca
    18901 tcagtgtgat ttcgtttgga tctaatttat aatctttggc taggcgattt ttccagtcga
    18961 tcagttcccc aatcatttca ataccggtaa atccagaacc acagacaacg aaagtcagca
    19021 tggctttacg cagttcatca tctggttcca atgcagcttt tgctactgtc ttctcaatat
    19081 gttcccgaat ccttacagaa tcttcaaatg accaaagggt gaatccgtgt gctttgacac
    19141 caggtgtacc aaagtcattc ggctctccgc ccattccaag gatcagctga tcaaaaggat
    19201 aggaacctaa tttggtcttg acgatttttc gttctttatc gataccgatt acagtgtctg
    19261 tgacaagttg tacattcttt tgacgagcaa acaaatgctg cagatcgtat tggattgctt
    19321 ctggttctac acgtccgcca gctacttcat gcaactcggt catcatagta tggtacgaat
    19381 gacgatcgat cagagtaatt gctacatctt cttttttcag tttctttgcc aagtattttg
    19441 tagctgaaac accggcatat cctgctccta caacgacaat attttgcttt gccattgatc
    19501 atccgctcct tatagactat ctatataatg aatatatgat aattctttta gtttgatact
    19561 tattacacaa tctccatata ttatatagat gttctgttct tttgtctaac tccttttgga
    19621 tttttttcaa attttaaaat aaagcgtttt ttcacgaaat ttgctgaaag aaaacgtttg
    19681 aaatattaca tgggttattt atcaaaaata gttgacttat aggctaaaaa gattagaatg
    19741 tataatgaat cacaaagaaa aattgtggac tagaaatgga atggtggggt attaaaatga
    19801 agaaaaaacg ttttgtaact ggatttgccg tattagcatt ttcagctcta gtgttaggcg
    19861 cttgcggcgc agatgacaac ggaagctcaa actcttcttc tgaatcatct acagcacaat
    19921 cttcgactgc aaaaagcaca acagaatcat ctgcaaaagt tgtagctggc ggagatttgc
    19981 aagacggcac atataaatta gaagaaaaaa attattcaaa tggttaccgt gcagtctttg
    20041 aaatggttgt aaaagacggc aaaatcactg aatcaaaata tgataacgtc aatgaaaacg
    20101 gcgaatcaaa aacaaaagat gcagaataca acaaaaacat ggaagcaaaa tctggcacaa
    20161 acccagaaaa atacattcct gagttgaatg accaattctt aaaagcacaa agcgcaagcg
    20221 gtgtggaagt cgtaactgga gctactcact catctgaaag cttccaaaac tacgcacaac
    20281 aattgatcca agctgctcaa gctggtaaca cagatacaat cgaaatcgac aatggtgctg
    20341 atttgaaaga cggcacttac aagcttgaag aaaagaacta ctctaacggc taccgtgtgc
    20401 aattcgaaat gacagttgct ggcggcaaag taactgaatc aaactttgat tatatcgaca
    20461 aagacggtaa atcaaaacaa gatgatacag aatataatga aaatatgaaa gcaaaaagcg
    20521 gtacagaacc aaaaacttac atccctactc tgaacgacga attcgtcaaa gcaatgggcg
    20581 aagaagatgg ctcacctgct aatgtagaag ttgttacagg tgctacacac agctcacatt
    20641 cattcatcat gtacgcacaa caattagtga atgcagctga aaaaggcgac actcaaacaa
    20701 tcgaagtaga taatatcgta acagaaaaat aatcacataa tcatactaat aaaagttgga
    20761 gggtgggacg atagccagat taggcagtcg tctcaccttt caaaatgaag agaggaaaaa
    20821 gatgcggaaa aaactactat tttccttaat cacactgggc atctctttta tggtgctaac
    20881 tggctgcagc aactcagcaa ataaaacaac cactgaaagt tcagcagcta ccaagatatt
    20941 gaaagatcca tattctgatg aacagttctt gttagggact tacgttcgta tccgtgtgta
    21001 tgatgaaggc aaagaatcag cattgaaacc tgccttcgat cgagtaaaag aacttggtga
    21061 taaaatcacg atcaatcaaa aaggttctga gatcgatgaa gtgaatgagc aagctgggat
    21121 caaaccagta aaagtttctg atgacgtcta cacgttagtc aagcgggcct acgaatatag
    21181 ccaagattct caaggcggat tcgatatggc gattggtgca atcacacaat tatggcgaat
    21241 cggttttgat gacgccagaa aaccttccca agaagaaatc gatcaggcat tgaaattagt
    21301 ggattatcat aagatcgaac taaatgacaa agaaaaaact gtttatctaa aagaaaaagg
    21361 aatgatcatc gatttaggtg caatcgccaa aggttacatt acggacgaag tagtgaaagt
    21421 cttgaaaaaa caaggagtga ccactgccat cgttgacttg ggcggtaatg tctatgtctt
    21481 aggccacagc ccaagaggcg aaaaccaaga ctggacagtc ggcatccaag atccgaacat
    21541 ggctcgcgga tctgttttag gttctattaa agaacggaac aaaacattag ttacctctgg
    21601 aatttacgag cgttatttag aagtagatgg caaaaaatac catcatttgt ttgatccaaa
    21661 aacgggttat ccgttcgaca atgatatcgc aagtgtcacg atcataacag ataaatcaat
    21721 tgatggtgac gggttgtcta cagcagtctt ttcaatgggt gtgaaaaaag gactggaata
    21781 cgtcgaaagc gaattgaata acgggacgga agcgatcttc gtgacaaaag acgataaagt
    21841 atatgtgacc gatccaatca aagacacctt caaattatcg gaggactccc attacacgat
    21901 gggtgaccgt tctgaactaa aataaggagt acgaaccatg acactaaaaa tctttttaga
    21961 agtagttgaa atacgaacaa aggtggctag tgtatttcct tttgcggtag gggtattatt
    22021 ctctatcgct ttttttcatg agatacattg gctaaacact tttttatttt ttattggaat
    22081 gctagttttt gatttagcga ctaccgctat caataattac atggattatg aaaaggctca
    22141 ttctgaagta tataagtatg aagaaaatgt cattggccgc agaaatctat ctcctgtatt
    22201 gattcgaaac atgattttag ctatgattgc atttgttttt gttatcggcg ttattctaac
    22261 gatcaatacg ggttggcttt tacttttgat gggatttatc tgttgtttta ttggagtatt
    22321 ctatacattc ggaccgattc ctttatctcg aatgccttta ggggaaattt tcagtggggt
    22381 gactatgggc cttggaattt ttgccatggt cgtttatgtc aatacattcg gttcagatgt
    22441 ctatgatctg acagtcaact tttcatctgg acaatttgat ttggtaggaa atctttgggc
    22501 tatagcagca atcgtattag cttccttacc cttggtgttt acgattgcca atatcatgtt
    22561 ggctaataat ttgcgggatc ttgaccgtga tattgaaaat catcgctata cactagtttt
    22621 ttatatcgga cgtcctgcgg gaacagtctt attccagcta ttgatgtatg cttgctattt
    22681 cgtgatttta atggggttgt tgagcggtat ctatgaatgg ccgatcttgg tcaccttctt
    22741 gaccttgcca attgtgaaga agcatttgga tcaattcaag caaacgttac cccagcctat
    22801 cagtttcagc tattccatca aaaatatggt cttattcaat gccagttatg tattggggct
    22861 attactaaca atcattatgc aagccattta acagcatgaa gaggttgtga aaaaactgcc
    22921 ctgcatcaag taataagaac agccaaataa aaatagctcc ttatattttt cgtttggttg
    22981 ggcttattac cgcagatgca agcttttgaa caccgtttat caggaggttg tgaaaaagtc
    23041 gcttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa
    23101 tttggcttaa taccgagaaa ctggcttttg aacaccgttt atcagaggtt gtaaaatcag
    23161 tgttttgtcc tgagtattag agaaacaaat ggaaaaatag ttcctcatat tttgccgttt
    23221 tttgagctaa taccgaagga cagctgattg aacactattt gttcggaaaa agtgactaac
    23281 tatgacacaa tttttttcac agtccctttt ttcacttgat ttgattcgta ccagcgaact
    23341 tgacgagaac tttcttggtt tggtaactat atggtattct tttaacatcc tcgtttgctt
    23401 cttctaattt atttatacaa agtcacactt ttttaaacat ctgttcttcg atacatttct
    23461 atttctttta aacatcgata ccaatatttt gaacttaaga gtgcatacca aatggattca
    23521 tttactcttc gtcagttacg aagataatca aaatctccaa cttccctact tatttcgttt
    23581 tgagaattat cgaagcaatc ttacataaat aactaaaaaa caaaaaacca gaaaatcagt
    23641 atactgattt tctggtttta catggtttgt tcaataaaaa gctgttggaa ctgttaatat
    23701 taaattatcg catcgttacg aattcttccg ctcctgttgg atggatggct actgtattgt
    23761 caaagtcttc tttagttgca cccatcttga tagccacggc aaaaccttgc agcatctcat
    23821 cgacaccaac accaatagca tgtaagccga cgattctttc ttcttttccg acacagatca
    23881 atttcatttc gcatttttgg cgataatcat ttaatgcaaa gtacattggg gtaaagcgtg
    23941 aacgataaac tttcaattca ttttcgccat atttttccaa tgcttcttct tctgtcaaac
    24001 caattgttgc aattggtgga tgcgtaaaga cgactgtcgg aacaagttta tagtcaagat
    24061 aagaatcttt ctttccatta aataaacgtt ctgataaacg tctaccagct gcgattgcaa
    24121 caggagtcag atctaattta ccgatcacat caccaacagc aaaaatatgt tcatccgttg
    24181 tattttggta tttatctact ttgataaacc cttttttgtc caattccaca tttgtatttt
    24241 ccagacctaa tttttcggta ttcggtacac gtccaccggc aaaaagtaca caatcaccag
    24301 ttaaggtttc accattttca aatagcaccg tatattcgtc attgttttta gtaatttctt
    24361 tagcaatatg atgtgcgtaa gtttttattc cagcttcttg gtacatttgg atcaaattgt
    24421 ccgaaagcat tttatcaaac gttctaagtg gtcgttcttt tcgatatgcc cacatgactt
    24481 cactacctaa accacgaaat acacctgaaa gttcagccgc aatgtatcca gcacctaaaa
    24541 cgattgctct cttaggtaac tctgttaatt caaaaaatcc atttgaatca attgcgtatt
    24601 ctccaccagg aatggtcatt ttacttgcac ggcctccagt agcgatcaag attttaggag
    24661 cacgataggt ttcaccgttt acttcgatcg tttgactatc gataaaagta gcgtaacctt
    24721 ttatagcctc gacatgattg ctatctaatc ctctttgata agcaccgtgt agaaaatcaa
    24781 tatatttttc tcgtctttct actagaccgg cgaaatcaac attttttaaa tctgcttgga
    24841 taccgtaact tgaggcatct cgttcaattg tttccaaaat cgtacttgct tgccacataa
    24901 cttttttagg tacacaaccg acgttgacac atgttccgcc caattcattc gcttcaatca
    24961 gcaaaacttt tgcgccatgc atccctgctc tgtttgctga agcaataccg ccagagccgc
    25021 caccaattac gatgtaatca tatatttgca ttttgttcct ccttattgaa gagatcaatc
    25081 aaaaccttta tttaacgatc ctttttctac agaaaacaag tgtaacactt ttcttgaatt
    25141 ttgtatgcga agatgcttaa aacagggata tatcagcttt ttccttattt acgttttcgg
    25201 atcatatctc caggtttcac cggctgtgcg actggcatag ttaaaatcat cattggattt
    25261 ggtgctcggt caatggattc gccttcttca ttatgcataa cttcaacggt ttgactgaaa
    25321 tgactgaatc ctggaccata aaattcgatt tcatctccta cagaaaacaa gtttctctga
    25381 cgtatcgtag caatctgtgt tgcctcatca taagataata cttcaccgac aaatttatat
    25441 actgggattt tacgacgttc accgaataat tgttcgtttt cagttggtgt attgtagtaa
    25501 aaacctgtag ctaattctcg ttgtgctact ttccataact cgtcgatcca ttcttgcttg
    25561 cagacatagt tttcaggatc ttccatataa ctgtctacag ccgctttata gacatttgac
    25621 accgtagata cataatggat cgatttcatt cttccttcga tcttgaagct gtctacgccg
    25681 ttttcaatca attctggaat atgttcgatc attgacatat ccactgcact catcgagaat
    25741 tcttcttcca cttcgcctgt atcagttaaa ctgtttcgtt cttgaccaaa aggcatatcg
    25801 aacaatccat acttccagcg acaagattga gaacatccgc cacggtttgc atcccgcatc
    25861 gacatatggt tggacaatgt gcatcgacca gaataagaga tacacatggc accatggata
    25921 aatgcttcga tttcggtatc cgtattttta cgaatctctg ccacttcctc cattgaaact
    25981 tcacgagcta aaacgacacg ttccaaccct tcttctttcc agaattccaa tgtctcaaaa
    26041 ttagttgctg aagcttgagt ggataaatgg atcggcagac ctggggcttc tgtcgcacaa
    26101 atttcgatca aagctggatc agaaacaatc actgcagaaa taccgacatc acgcaaactg
    26161 cggaaaaatt cccctgctcc atcttcattt ccttcatggg tgaccatatt agcagcaacg
    26221 tataccttcg catcatgttc ttttgcaaaa gcaactcctt cagccatttc ttcataagta
    26281 aaattacctg cacggcttct taaaccatat gcattcccgc caatatatac tgcatctgct
    26341 ccataatgga tcgcggtttt taacttttcc agtgttcctg ctggcgctag gatttctggt
    26401 cgtttcaatt ttctttttac tgtcatttct ttcatcatac tccttatttg atagcagaag
    26461 gatcaatata aaagaatcct gtatctaatt cacggttttc aggatgcaat ttactgattt
    26521 cgctagtcat tttttccgct ttttcttgat cccagctacc ttctaaaatt tgtttttttg
    26581 cttcggcgaa tagtcgagtg atgtttacga atttttcgcc tggagtaaat aatccgtcca
    26641 atttccatgt acagtaatga tgctgataaa gttcattcaa ttcattcgag agattcagat
    26701 cattgctggc aaaaatatgt gtgccatgac tatcttcata aatagaataa tgtgtctctt
    26761 cttttttagg ttccgagata aaaagacctc tttcacgatc tttttgttcg tcttgtttcg
    26821 tatagttata gtaattctgt agcagtggac gtttggattg atggatgcat gtagcaccat
    26881 acacaagggt ttctacagga atatcgagtt tttcttccat cgccttcatc tcttcaaaag
    26941 gaacttcacg agcaagtact gctccaactg cgcctttttt cgcccagaaa ttgatttgac
    27001 gtgcgctcgt tactaatgtt tcagcatcat aaatatacgg tatacgcaat tcaggatttt
    27061 ttttcatcgt ataaataata cctgtatcac caactgtgat cacatctacg tttatttctt
    27121 ttaaaaaagc caaatattca ggtacatttt tcattttttc aggatgaatg atcccattta
    27181 cagcaatatt cgctttttta ccatacttgt gtgctaattc taccaattgt cgctgttctt
    27241 ctcgtgtaaa tgaagcaggc agacgcaatc caaattgctc ttcaccgaaa tatagtgtat
    27301 ctgcacctgc agcaagcaac tgttctgctt gttcaatagt ttctactgtg gttatgagtt
    27361 cgatcataat tttctcctcc tacaacttca taatagtagc atagctctaa aaactttcca
    27421 atacttcttc tatttaaagt cggatttttg aaaattattt cacaaaaaat caaaataaag
    27481 ttcgatatat tctgtccaaa tataaaaaga gattgtgaca aaaatcttgt cacaacctct
    27541 taatcaagtc ttactattat aaaaattcag ctacacaggc caagtaacta tagggaacct
    27601 actgtttcct ttttaggtcc gcgaacattc aatgtgatct taccttttga agcacctact
    27661 gttacgtggt cacctaaacg aatttctccg ccaagcaaag cctcgctcaa tcgatcttca
    27721 atttcttttt gaagtgcgcg acggattgga cgtgcaccat attcaggatc aaatccagct
    27781 ttcccgatca catcgattgc agatggtgtg attttcactt gaatatcttg ttctttcaat
    27841 cgtttgataa tcgctttgct catgatttgg acgattgtat ggatttcatc tttactcaat
    27901 gaatggaaga cgacagtctc atcgattcga ttcaagaatt caggtcggaa agctttcttt
    27961 aactcttcta agatacgttt ttgcatggct ttgtgatctt tagtaacatc ttggacattg
    28021 aatccgacat ttttttcttc tcgaatttgt gtcgctccga tatttgaagt cataatcatg
    28081 attgtatttc taaagtccac ttttctacct ttagaatctg ttaaatgacc atcatccaat
    28141 acttgcagaa gtaaattgaa aacgtctgga tgtgctttct ccacttcatc taagagaatc
    28201 actgaatatg gtttttgacg aattttttct gtcaactgtc caccttcatc ataccctacg
    28261 tatcctggag gagacccgat taatcggctt gtactgtatt tttccataaa ttctgacata
    28321 tcaacacgga tcagggcatc ttcacttcca aacatgactt ctgaaagtgc tttggccaat
    28381 tctgtttttc cgacaccagt tggtccaagg aacatgaatg aaccaattgg acgatcagga
    28441 tctttcaatc cgcttcgtgc acggcggatc gcacgtgaaa ctgctttgac agcctcatct
    28501 tgaccaacga cacgttcatg cagaagtcct tccagttcta acaagcgttc gctttctttt
    28561 ttctccaact gctgtagagg gacaccagtc cactccgata caactgtagc aacatcttct
    28621 tctgttacac gatcagaata accttgctcc tgttttgctt caattgcaga aactttttgc
    28681 agctttttac gtagtgcttt ttcttgtctg cgtaattgag cagcattttc aaagtcttgt
    28741 ttttggatcg ctgcttcctt ttcttgtact agttcatcga tttctagttt gatcacagtt
    28801 gattttgtta agtgatcggt ttgatccaaa cggactttcg ctgctgattc atcgatcaaa
    28861 tcaattgctt tatcgggtag ttgacgagaa gtgatataac gaacagaaag attgactgct
    28921 gctcgtacag cttcatctgt gatctccact ccatggtgct cttcatatct tgtacgtaat
    28981 ccttgaagaa tcacttctgc ttcttcagga gttggctcat ctacttgaat gcgagcaaaa
    29041 cgtcgctcta atgcagaatc tttttcgata tatttttgat attcatctag ggtcgttgct
    29101 ccgatcgttt gtaattctcc tcgagctaac gccggtttca agatattcga agcatcaatt
    29161 gctccttctg ctccacctgc accaattaag gtatgcaatt catcaatgaa taagattact
    29221 tgaccatcat tatagatttc gtcgatgacc tttttcattc gatcttcaaa ttcaccgcga
    29281 tatttcgtac cagcaaccaa agatcccata tcaagcatca tcaggcgctt ttcttgcatg
    29341 tcttctggca cttcacctaa aatgattttt tgagctaacc cttcagcaat ggctgttttg
    29401 cctacaccag gttcacccac cagtacaggg ttattctttg tccgtcggct aagaatctgg
    29461 ataagccgtt tcacttctct cgaacgacca acaactgggt ccagcttgtt ttcttttgct
    29521 aatttcgtca aatcacgtgc taatgaatct agcgtaggtg ttccttgcgg tgtttgccgt
    29581 ttatcttgta catttacatt tctacgacga ttcattcctt ttccagcaga atcaccagct
    29641 agccccattt tttgtttcag aacttgacgg gcttttgaaa ggctgatacc taagttgatc
    29701 atgattcgag tagccatgat actttcatct tttaataagc tcatcaaaat atgttctgtt
    29761 ccaacttttt gagaaccagt tttcttcact tcgctgctag ctaaagcaaa aacttgtttc
    29821 attcttggtg aatatggaag aaagactcct ttagggtaag aacggatacc accataacca
    29881 atcagatgtt caatttcttc ataaatttcc gtttcattca aatccatttc acgtaatact
    29941 tttccagcaa ttccattttg ctcgaccact aaggctagta aaacatgttc tgaactaact
    30001 gattgatgtt tcaaccgttt tgcttcttct tgtgcaattt ctaatactgc ttgggctctt
    30061 tcagtaaaaa gttcttccat ctaatctcac ttcccatctt cataacttaa acgttccaga
    30121 aatgctcgga gaatccgagc acgtgtatga tcttcatatt cattgaaatt caatgtgttt
    30181 tttgcaattg cactcaacat caagtttcct tctttttttg taattatttg gtcttcataa
    30241 agtttttgaa taatagcaaa agcatttttc tcactgattg tatcatcaaa taactgattt
    30301 atctgctcaa gtaattgttt tttatctgaa atccttactt ttgcaattcg tatataacca
    30361 ccaccaccac gtttgctttc tactagataa ccacgttgga tggtaaaacg ggtattaatc
    30421 acatagttga tttgtgaagg aacacagtta aagaggtctg ccatttcaat ccttcgaatc
    30481 tcgatcattt cactattttc cagaattttc tttatatatg attcaattaa atccgaggta
    30541 ttctgatgtg ccatcttatt cattcccttt gactaatatt gaccttaatt ataacgaatt
    30601 tgataataga aagaaagaag aacgcttttt gttaaataaa tcctttagaa tcaacaaaaa
    30661 aaaatacgga atatactatt ccgtatc
//
LOCUS       DBKOIJ010000016        30525 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000016 DBKOIJ010000000
VERSION     DBKOIJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.016"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..210)
                     /locus_tag="ACV5NJ_000569"
     CDS             complement(<1..210)
                     /locus_tag="ACV5NJ_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484692.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISS"
     gene            complement(198..827)
                     /gene="pyrE"
                     /locus_tag="ACV5NJ_000570"
     CDS             complement(198..827)
                     /gene="pyrE"
                     /locus_tag="ACV5NJ_000570"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HHY6484693.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(834..1550)
                     /gene="pyrF"
                     /locus_tag="ACV5NJ_000571"
     CDS             complement(834..1550)
                     /gene="pyrF"
                     /locus_tag="ACV5NJ_000571"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HHY6484694.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1547..2479)
                     /locus_tag="ACV5NJ_000572"
     CDS             complement(1547..2479)
                     /locus_tag="ACV5NJ_000572"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HHY6484695.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2476..3252)
                     /locus_tag="ACV5NJ_000573"
     CDS             complement(2476..3252)
                     /locus_tag="ACV5NJ_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HHY6484696.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3306..6491)
                     /gene="carB"
                     /locus_tag="ACV5NJ_000574"
     CDS             complement(3306..6491)
                     /gene="carB"
                     /locus_tag="ACV5NJ_000574"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HHY6484697.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6496..7572)
                     /locus_tag="ACV5NJ_000575"
     CDS             complement(6496..7572)
                     /locus_tag="ACV5NJ_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HHY6484698.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7577..8857)
                     /locus_tag="ACV5NJ_000576"
     CDS             complement(7577..8857)
                     /locus_tag="ACV5NJ_000576"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HHY6484699.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8876..9802)
                     /locus_tag="ACV5NJ_000577"
     CDS             complement(8876..9802)
                     /locus_tag="ACV5NJ_000577"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HHY6484700.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9815..11098)
                     /locus_tag="ACV5NJ_000578"
     CDS             complement(9815..11098)
                     /locus_tag="ACV5NJ_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HHY6484701.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11112..11651)
                     /gene="pyrR"
                     /locus_tag="ACV5NJ_000579"
     CDS             complement(11112..11651)
                     /gene="pyrR"
                     /locus_tag="ACV5NJ_000579"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HHY6484702.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11886..12797)
                     /locus_tag="ACV5NJ_000580"
     CDS             complement(11886..12797)
                     /locus_tag="ACV5NJ_000580"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HHY6484703.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12794..13258)
                     /gene="lspA"
                     /locus_tag="ACV5NJ_000581"
     CDS             complement(12794..13258)
                     /gene="lspA"
                     /locus_tag="ACV5NJ_000581"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HHY6484704.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13263..13961)
                     /locus_tag="ACV5NJ_000582"
     CDS             complement(13263..13961)
                     /locus_tag="ACV5NJ_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HHY6484705.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13948..14334)
                     /locus_tag="ACV5NJ_000583"
     CDS             complement(13948..14334)
                     /locus_tag="ACV5NJ_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484706.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14481..16151)
                     /locus_tag="ACV5NJ_000584"
     CDS             complement(14481..16151)
                     /locus_tag="ACV5NJ_000584"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HHY6484707.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16334..16567
                     /locus_tag="ACV5NJ_000585"
     CDS             16334..16567
                     /locus_tag="ACV5NJ_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HHY6484708.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16666..17097)
                     /locus_tag="ACV5NJ_000586"
     CDS             complement(16666..17097)
                     /locus_tag="ACV5NJ_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HHY6484709.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17176..17589
                     /locus_tag="ACV5NJ_000587"
     CDS             17176..17589
                     /locus_tag="ACV5NJ_000587"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HHY6484710.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17627..18775)
                     /locus_tag="ACV5NJ_000588"
     CDS             complement(17627..18775)
                     /locus_tag="ACV5NJ_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HHY6484711.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18897..19493)
                     /locus_tag="ACV5NJ_000589"
     CDS             complement(18897..19493)
                     /locus_tag="ACV5NJ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHY6484712.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19693..22335)
                     /gene="alaS"
                     /locus_tag="ACV5NJ_000590"
     CDS             complement(19693..22335)
                     /gene="alaS"
                     /locus_tag="ACV5NJ_000590"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HHY6484713.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22723..23154
                     /locus_tag="ACV5NJ_000591"
     CDS             22723..23154
                     /locus_tag="ACV5NJ_000591"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6484714.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23199..24548)
                     /locus_tag="ACV5NJ_000592"
     CDS             complement(23199..24548)
                     /locus_tag="ACV5NJ_000592"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHY6484715.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24618..25781)
                     /locus_tag="ACV5NJ_000593"
     CDS             complement(24618..25781)
                     /locus_tag="ACV5NJ_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HHY6484716.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25910..26233
                     /locus_tag="ACV5NJ_000594"
     CDS             25910..26233
                     /locus_tag="ACV5NJ_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484717.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26416..27765)
                     /locus_tag="ACV5NJ_000595"
     CDS             complement(26416..27765)
                     /locus_tag="ACV5NJ_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HHY6484718.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27834..28787)
                     /locus_tag="ACV5NJ_000596"
     CDS             complement(27834..28787)
                     /locus_tag="ACV5NJ_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HHY6484719.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28889..30079)
                     /locus_tag="ACV5NJ_000597"
     CDS             complement(28889..30079)
                     /locus_tag="ACV5NJ_000597"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HHY6484720.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(30279..>30525)
                     /locus_tag="ACV5NJ_000598"
     CDS             complement(30279..>30525)
                     /locus_tag="ACV5NJ_000598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484721.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         8580 a         6694 c         5355 g         9896 t
ORIGIN      
        1 agaagagatt attctcttct ttctgctaac cagtcttaga aaaatgaatt ttaaaaaaat
       61 caaaaactga tcacgataga agtttctttt catacctgat gaaaagatgc ttctcgcaat
      121 cagtttttgg atttttgcta ttccaattga tgattctgga gacacaaatc ggatttctct
      181 atcttcatca agcgcgctta aatctgccaa ttttcaggat cttttttcca ttcttttagc
      241 agagttaatt cttgctcatc gatataacga tcttctaaag ctgcttcaat caatacagaa
      301 taattggtca acgtcataag agggatttcg gctttttcga aatttgcttt tccttttggc
      361 aactcatacg taaagatagc cgcaacacca aggatatccg caccttctct ttttgctgct
      421 tcagctgctt ccaagacact tccgcctgta gaaatcaaat cctcgatcac aaccatcttc
      481 tgaccttcaa atatacgtcc ttcgatttgg ttacctttcc catgatcttt cgccttactt
      541 cgaatataga ccattggcaa atccagtatt tccgctaccc atgcggcatg agggattcct
      601 gctgttgcag ttccagcaat cacttgtact tcagggtaag cttctttgat tttgcttgct
      661 aatccttttg cgatctcttt tcggactttt ggatagctca tcgtgattcg gttatcacag
      721 taaattgggc ttttgattcc acttgcccat gtaaaaggat tacttggatt taaaaatact
      781 gcttcaattt ctaataagtc ttttgcgata ctttgttcaa cattcatttt aggttagctc
      841 cattctgttt gaatcgtttg atatgcttta taaggattag ttgactgagt gattggtcga
      901 cctataacga taaaagttga gccgatcttt tttgcctgag aaggtgtgac tactcttgtc
      961 tgatctcctg cgactgttcc ttctggtctt atacccggag ttaagcaaac aaattcgtct
     1021 cctgccattt ctttgatcgc ttctacttcc caagcagaag aaaccacacc atctaatcct
     1081 gcttttttgg cacagctagc ataatggatc acactttttt ctaaagaaac ttcgattagt
     1141 tgatcggcat gcatttcttc ttcacttgta gaagtaagct gagtgatagc tagaagtagc
     1201 ggacgttttc caccttctgg agtcccctct tctaatcctc gcattgccgc ctccatcata
     1261 cgaataccgc cagcggcatg aacacaggtg agatcaacac caagctttgc caatccccgc
     1321 attgcttttt cgaccgtatt cggaatatcg tgaagtttca gatctaaaaa tacattatga
     1381 ccagcctctt ttagataacg tacgatttca ggtccttctt ggtagaacaa ttccattccg
     1441 attttcacaa acaatttttc ttctttcggg aaaaatcgaa gaaagtcttc tacctcttgc
     1501 ctagttgaaa aatctaatgc aataattggt cgttggtcta gttgattcat cgttcacgtt
     1561 cctctctcac ttctttgatt aatctttcca gcgattcgat gcctagttcg ctcattcgca
     1621 cagggagctc tttaattagt ttaggacaga tatacggatc ggtgaaattc gcggtcccta
     1681 tagctacagc acttgctcca gccataaaca tctcaagaac atcatccact gtctgcacgc
     1741 cgcccatccc aatgattgga aggctggata tacttgctac ttggtgaatc aaacgaatag
     1801 cgacaggctt gattgctggt ccggagagtc cacctgtttg attggctaaa atcggttttc
     1861 ttgtcttaag gtcaatccgc ataccaagaa gagtattgat catcgtaaac ccatctgccc
     1921 ctccagcttc gattgcctga gcgattggaa cgatgtctgt tacatttggg gacaatttga
     1981 catagacagg aacagaagaa actttcttta ctgcttgtgt taattgaaaa gcaacatcag
     2041 aatctgttcc aaaagcaatt ccgccatgtt tgacatttgg gcaagaaatg ttcagctcga
     2101 ttgctttcac atttggtgct tcgccaattt tgctacaaac ttctacatag tcttcttcac
     2161 aagcaccagc aacatttgcg atgatgggta actcattgta ctcctctaat tctggaagaa
     2221 gtttatgcat aacaacgtct agaccaggat tttgcaaacc aattgcattc aacatgccag
     2281 atggtgtttc cgccactctt ggcgtgggat tgccaaagcg ggcatttgga gtcgttgcct
     2341 tgaccatgat cgatccaagc tgattcaaat catagtattt tccgtattct ttgccaaaac
     2401 cgaaacagcc gcttgccggc atgataggat tttttaattc taggccagga agtttgaccg
     2461 ctaaaggatt ctcattcata taattacctc tcctatttta aaaatcggtc cttcgtcaca
     2521 aactttcaca ctttttgttc ctgtttcatc ttccggcgta tgacaaacgc aagcatagca
     2581 agcccctatc ccacatgcca tgcgggattc taatgataac tggacatttt gatgcgaaga
     2641 aaacagctgt tccactgtct tcaataaccc gtttgaacca catgcaaaaa cagctgtagg
     2701 ttccgtcttc gttcctaaca ataaattacc gacatttccg tgcataccgt atgttccatc
     2761 gtcagtagcg attcttgttt ctcctagacg attaaattct ttttcataat aaacgacttc
     2821 atgtgtggca aaacctaaaa aatgcgttgg cttaattcct ttttcgacta actgttttga
     2881 taactcatat agaggaggga caccgattcc tccaccgatt ataaatactt cgtctcctgt
     2941 atgaagattg gacaaatcaa aaccattgcc taaaggaccc atgacatcta aatggtctcc
     3001 ggcagaaaga gtagagaaga ctctagtccc ctcaccttcc acccgataaa tgatacggca
     3061 agtatttgtt tcgtggtcga tatgattcaa gctaatcggt cggcgtaata gcaagtctgc
     3121 acgcggcacg agaatatgca agaactgtcc tggttgctgc atttctttta ctagatcacc
     3181 tttcaatgtc atttcaaaaa tctttggtgc cagctgtttt tgctgaacga tttccatcat
     3241 ttcttgtttc atctgtcccc tcctgcaacg gtttttcgtc tttttgtctt aacatgtgct
     3301 cgccgttaaa tagggttagt tgaaaaagca cgggattcca tcactcgaac gatcgcatct
     3361 gctgtgtcta aagaagtaaa caatggaata ccatgttcaa ctgcttctct tcggatgatg
     3421 aaaccgtctt tagaagcatt tccccgatct ttatcaatcg tattgacaac cacttgtgtt
     3481 ttaccatctc ttattaagtc gactacattt ttttctgctg cttcctcaga aattttcgct
     3541 actgtttcaa cgaccagacc atttttttcg aaataagcag ctgtatgttt cgttcctatc
     3601 agactatagc caatttctgt aaaacgttta gctaattcta gtgcttcttc ttttgtttcg
     3661 tccgcaatcg taaataatac ggctccatat tcaggcaagt gtaatccaga agcttcgaat
     3721 gccttgtaca aagctttgtc taattgtctg tcagatccca ttacttctcc agtcgatttc
     3781 atttcaggcc ctaagtaagt atcaactttc tgcaatttcg taaaagaaaa gacaggtgct
     3841 ttaacatgaa cttcttttgt ttctggataa agtccgtttt cataaccaag tgtctttagt
     3901 ttctctccca aaatagcttt cgtcgcaatt tgtgccattg gaattcctgt caccttactt
     3961 aagaatggaa cagtccgact tgctcgagga ttgacttcga tcacataaac ctcattttca
     4021 tggatgacaa actggatatt catcatcccg acacaattca aacctaatgc taagcggatc
     4081 gtgtaatctt cgatcgtttt cttgatcgat tcagataatg tttgaggtgg atacacagcc
     4141 attgaatccc ctgaatggac accagcacgc tcaatatgct ccatgatccc tgggatgagt
     4201 actgtttcac cgtcacaaat cgcatccact tcgcattcac caccaagcaa gtaacggtcc
     4261 actagtactg gatgttctgg agatgctttc acagcatgtt ccatgtaatc ttctaaatct
     4321 cgctggtttt caacgatttc catcgcacgt ccgcctaata catagcttgg acgaacaaga
     4381 actggataac caattctcga agcgataacc acggcttctt ctttacttgt agcagtatcc
     4441 cctaaaggtt gaggaatatc caactctttc agcgcttgct cgaataaatc acgattttcc
     4501 gcacgatcca agtcttcgat cgttgtccct aagattttta caccagcagc agtcaaaggt
     4561 tcggctaagt tgattgctgt ctgaccacca aattgaacga ttactcctat cggctgttct
     4621 agctcgatca cattcatcac atcttcgaag gtcaatggct caaaatataa tttatccgaa
     4681 atagaaaagt cagtcgaaac tgtttcagga ttgctgttca tgatgattgc ttcgtatcct
     4741 gcctgctgga ttgctttgac agaatgcaca gtcgcatagt caaattcaac cccttgtccg
     4801 atacggatag gaccagatcc cagtactaga attgagggct tttctgatcg tgaactttca
     4861 ttttcatatt cataggaact ataaaaataa ggtgtttttg attcgaattc cgctgcacaa
     4921 gtgtcaacca tcttatatac aggaatcaaa tcattttctt ttctcatctg gcgaacggct
     4981 tcttcgttta tttcccacag ctccgccact tttttgtctg taaaaccgtt ttgtttcgct
     5041 gttttcaata tcttgtcatc caacggctgc tgtttcaact gctgttcgat ttctacgata
     5101 tgcaacagtt tatctaaaaa gaataaatcg atttttgtca aatccgctaa ttcttctatg
     5161 gtgtaccctc ttctaatagc ttcagctaca taaaacaggc gatcatcttg tgctcgaacc
     5221 actttttgga ttaggtcatc gtccgtgatg tctgccaact caggaagttc gatatggtgt
     5281 gtaccgattt ctaaagaacg cacagctttc aataaagact cttcgatatt ccgaccgata
     5341 gccatcactt caccagttgc tttcatctgc gtgcctaaaa gccgttctcc gttttcaaac
     5401 ttatcaaacg gccagcgagg aattttagct acaacataat ctaatgcagg ttcaaattct
     5461 gcaaaagtcg ttcctgtaac aggatttttc atttcgtcta atgttaatcc aagcgctatt
     5521 ttagcagcta gcttagcgat cggatagcca gtcgccttac ttgctaacgc agatgaacga
     5581 gaaacacgcg gattgacttc aatcacataa taattaaaac tatgtggatc aagagcgagt
     5641 tggacattac agcctccctc gatcttaagt gctcgaataa tcgaaagaga agcatcacga
     5701 agcatttgat attcatggtc agacaatgtt tgactagggg caaatacgat agaatcacct
     5761 gtatggatac ccaccgggtc aaaattttcc atattgcaga caacgattgc attatcagcc
     5821 gaatctctca tcacttcata ttcaatttct ttaaaacctg caatacttcg ttcaatcaaa
     5881 cattgggtag cgggggaaag acttaatcca ttttctgata cgatacgtaa ttcttcttca
     5941 ttttcacaca tacctccacc agttccgcct aaagtaaagg ctggacgaac gatgacagga
     6001 tagccgattt tatttgcaaa atctacagct tcttctactg tattgacgat ttcagactcg
     6061 gggattggct gattcaattc tttcatcaac tgtttgaata gatcacgatc ttctgcttga
     6121 tcgatcgcag aaagctttgt tcctagaagt tctatgttca attcttctaa aatacctgat
     6181 gctgccagct ccattgccat attgaggcct gtctgtccgc ctaaagttgg aaggagagca
     6241 tctggacgct cttttcgaag aatccttgat acaaattcaa gtgtgatcgg ttcgatatat
     6301 acttgatctg ccacttcttt atccgtcatg atcgtcgcag gattagaatt tactaaaacc
     6361 acttcatatc cttcttcttt taaggcaagg caggcttgtg ttcctgcgta gtcaaattct
     6421 gcggcttgac caatgatgat cggaccagat ccaatcacca taattttctt gatatctgta
     6481 cgtttcggca tctgtttact gctcctttcc tgcgtccatc atttccataa attcatcaaa
     6541 taaatgtagt gcatcatgcg gaccaggggc agcatctgga tgaaactgca cactgaaagc
     6601 gggataatct ctgtgtttga ctccttctac tgttccgtca tttacttcta catgagttac
     6661 gatgagtttt tctggatcaa tcgtggcttc atcgactgca tagccatgat tttgagaagt
     6721 aaaatcaatt cttccggtcg ctacttctcg taccgggtga tttaatcctc gatgtccgaa
     6781 tttcatttta taggtatctg caccgttggc taaagcaaat aactggtgac cgagacaaat
     6841 accaaaaatc ggtacttttc cttggatttt tttgatcatt tcgattgctt ctggcacatc
     6901 ttttggatca ccaggtccat tcgtcagcat gactccgtct ggtgatagcg ctaaaatcgt
     6961 ctcggcatct gtgttatagg gaagtactgt gaggttacag tttcttttac tcaattctcg
     7021 taaaatacta tgtttcaagc cgaagtcaat gacaatgact ttccgacctg ttcctggact
     7081 tggataaggt ttgttagttg agacttgctg cacttgattg gttgctagaa cggttgcttt
     7141 cagctgatca aatgcatgtt cgaaggaatc tccagcatca actatactag ctttcatcgt
     7201 tccgacttca cggattttgc gagtaagggc gcgcgtatcg atacctgata ttccaggaat
     7261 tcctttttgt ttcaaaaatg aatctagcgt catttggttt cgccagtttg aagctacacg
     7321 tgcatgttct tttactacta ctcctttgca tgttggagaa atcgattcat aatcatctct
     7381 atttacccca taattaccca ccataggata ggtaaatgta atgatttgac cgttaaaact
     7441 ttgatcagtg atagtttctt gataaccagt catacttgtt gtaaaaacga tttctccgaa
     7501 gacactagca tctgcaccaa aagcctttcc ttcgaaaact gttccatctt ctaatataag
     7561 caaacgttcc atacttttat tctcccttct gccacacaag ttttccgttg acaaaagtca
     7621 tttgtgtttc gccttttact ttccagccga taaaaggtgt attctcccct tttgataaaa
     7681 agtcttcttt atcgatcgtg caagtttgtg taatatcaaa aactgctaca tcagcggggg
     7741 caccaaccgt tagcgttcct gcattcaaac caaaaatctc agccggttta actgccaacc
     7801 aattgatcac ttgttccaac gtgaatcgac cagtttctac aaaatgagta tagatcagtt
     7861 gaaacgctgt ttcgcttcct acgataccaa atggtgattt catgaaactt tggctttttt
     7921 cttctaatcc atggggggca tggtctgtag cgatgcaatc gatcgtaccg tcaagcaagc
     7981 cttcgatcaa agcttctctg tcctcccgtc ctcttagcgg cgggttcatt ttccaaaagc
     8041 cgaaatcttc gggaatatct tcgtcgatca aaataagatg atggggtgaa acttctgctg
     8101 ttacgtggat tcctgctttt tttgcgtctc tgattacacg gacactttct ttagtagaaa
     8161 catggcaaac atggtaatga acaccagttg cttcagctag caacagatct ctagcaattt
     8221 gagaagactc ggttacgctt aagatcccgg gaagaccaag ttcttctgcc ttcttgccag
     8281 cgtgcataac gccgccaaat aataaagatt catcttctgt atgggctact agggctttat
     8341 tgttttttgc agcttctttc atagccagat acatcgtccc agctgtttgg acacctacac
     8401 cgtcatttgt gaaagcaaaa gcaccttctt caatcagtgc ttcttgatca actaatttct
     8461 cacttcgcag attttctgtg attggcgcat actgcaatac cttcacaact gcatcttttc
     8521 taatgatgtc atatacttgt ctcagctttt ctgccgtatc aggtacagga tttaaattcg
     8581 gcatcgcgca tacagtagtg aaaccgccac gagcagctga gcgagtccca gcttcgatcg
     8641 tctccttgta tgtaaatcct ggctctctca aatgaacatg gacgtccact aaaccaggtg
     8701 tgatcaattg tccctttgca tcaaacactt catcaaattc ggcttctgag aatcctgtac
     8761 cgattgcttt gattcgccca tcttctatcc aaatttctgc tggtgtcgtc atattttttc
     8821 gtgtattgat ctgaccgttt ttaatgagtg ttttcatttt ttcatcctcc tataattacg
     8881 cttttccatt tagcactgct tctaagattg ccattctgac gaatactcca ttgctcattt
     8941 gctgaacgat ccgcgactgt aatccttcca ctaatgaatc agctaattct acatcacggt
     9001 taactggtgc tgggtgcata atgattgcat gtttttgcaa cttcttcgca cgctcaactg
     9061 ttaacccatg ttcctgatgg tagccttctt ttgagaaact ttcttttcca tcatggcgtt
     9121 cgtgttgcac ccgaagcatc atcatgacat caacttgatc tagtagttca tccaatggca
     9181 tataatgtcc gtacacttcg aattcatcgt cgtaccattc tctaggacct gagaagaata
     9241 cttgtgcgcc tagacgtttt aacatctgca tattggattt agctacgcga gagtgagtga
     9301 tatcacctac gatggcaact ttcagacctt caaaatgacc aaattcttca taaatggtca
     9361 tcaaatcaag caagcattgg gttggatgct gtccgcttcc gtctcctcca ttgataatcg
     9421 aacactggat tgttttactt tgtatcagtt cgtcataata gttctcatca ccatgacgga
     9481 taaccgctac gtctacgcct aatgctgaca tcgtcagtac tgtatcgtat agtgtttccc
     9541 ctttttggac agagcttgtg cttgcttcaa attcaatcac ttctagccct agttttttct
     9601 ccgctacatc gaagctttta tgtgtgcgag tactattttc gaaaaagagg tttgtagcaa
     9661 agtactggcg cttttgcgga gtccaattag caccttgctt gaattcctgc cctctgcgga
     9721 tcaagcccat aacttcttga tcagtaagtg cctcaacggt caataaatgc tttaagctga
     9781 tacgttctga tttgatgatc ataatgtttc ctccttagtt ttcttcgctt cgtgaagttt
     9841 ctggtaagat aagatttaaa atgatgccaa gtacagttgc taatgccatt gccgacaatg
     9901 taaatgtgcc tacttcaaag acgagtccgc caattcctac tactaagata actgaagcaa
     9961 tcaacagatt tttcttttta tcaaaatcga ttttgttctc gattaaaatc ttcattccac
    10021 ttgcggcaat tactccaaat aagataaaac tgattcctga gatgactggt ccagggatac
    10081 ttaaaatcaa agcgcttaat tttcctacga atcctaaaat gactgcaaat actgcagctc
    10141 caccgatcac aaacacgcta tggacacgtg tgatagccag aacaccaatg ttttcaccgt
    10201 aacttgttac aggaggacct ccgacaaagg ctgccacgat ttgtgctaat ccatcacctg
    10261 tcaacgtctt atgaagaccc ggttcttcaa agaaattacg tttagttagt ttattcaata
    10321 ccataagatg gccgatatgc tctgtcatcg tcacaaaagc gatgggtgcc attgttgtga
    10381 ttgctccaag atatagcttt ggttcatatt gtacaaaggg aatctcaaag ttaggaacag
    10441 caaaccaggc agcgtctacg attggcttag tatccacgat tccaaacagc aaggctacga
    10501 tgtatccgct aataatacct agtaaaatcg gaatcaatcc gagaaaacct cttagccaca
    10561 tgttgaagaa aattgttaat cctaatgtga tcagcgctac agcaatatac ttgaaatcat
    10621 acgtgccctg attatacata gcatttgttg ctgcatttgc cgcaagtccc aacccgataa
    10681 ccatgatgac tggaccgacg acgattggag gcaagatttt atccagccat gctgaaccga
    10741 tctttttgat gatcagcgca acaatcaaat agactaatcc agttgtgact gctccttgtg
    10801 caatcgctgg ataaccatct gttttcatca aaagctgcat cgctgcaatg aaagcgaagc
    10861 tgcttcctaa gtacgcaggt atctttcctt tagtagtaaa taagtacacc aatgtaccta
    10921 gtcctgaact gactagcgca atacctggat cgatacctac taagatgggg actaacacgg
    10981 ttgctccgaa catcgtgaac aagtgttgta aacttaatcc tacccaatgt cctggggttg
    11041 ggcgatcgtg gatatctaac accacgtcat cattatgaaa tccttttttc tccgacatgt
    11101 ttctctctcc tctaatcttc ctctttcaaa atcatgatgc gatcttgtcc atcaagctct
    11161 tgcatctcta ctaaaatctc ttccgcccga gatgttggaa tgttcttccc aacataatct
    11221 gcacggattg gcaattctcg atgcccacga tcgaccagga ctgctaatga aatttttctt
    11281 ggtctgccaa aatccattac tgcgtccatt gctgctcgaa tcgttcgtcc tgtatacaaa
    11341 acatcatcaa tcaaaataac ttcttttcct tctaaggaaa ctgggatgtc agaagaatga
    11401 agttccggtt cttctggatt ttcttttttg tcatctcggt ataaagtgat atccagttct
    11461 cctacaggaa tatcgatatc ttctagctgc ttcaggcgtt cggcaattct tgatgcaatg
    11521 tagattcctc gtgttttgat acctaccaat acgatatctt ggatactatg gtttctctcg
    11581 ataatctcat aagtaattcg agtcaatgct cgcttcatgg ttacctgatc tactacttct
    11641 tttgcttgca ttttcattcc ccctactttt ttgcataaaa aaactcctgc cctaagagga
    11701 caggaggaat gtttagactg agcgtatact gatacactga tagctatact atgtcaatga
    11761 gcaatctact tatggcgcac catacgtatc cttgctccca cccttcttag cctcacggga
    11821 ctaaacttaa aggaattcta ttaagtttta tgctttcgtt gttaattact ataccgttat
    11881 ctaaattaaa atgcaatgtt ttttcttaat ttttctaaag ttttttcaaa aacttctgga
    11941 agaggtgctt caaagatcat cttttcgttt gtagtcgggt gcgtaaaacc taacatttgc
    12001 gcatggagaa actgaccatt gcctttcaaa gttttcttcg gtccgtatac tggatctcca
    12061 gctaacggat aaccgatata cttcatatgc acacggatct ggtgtgttct tccggtttct
    12121 agttttaatt caaccagagt aaatccttcg aaacgttcca atacagtaaa atgtgtgaca
    12181 gccgacttcc catcttctct tatcgcctgc attttccggt tttccttcga acggccgatt
    12241 ggcgcattga tggttccttt ttcgtgagga atctctccat ggactaaagc aacatatttg
    12301 cgcaaagagg tcttatcttt taattgctga gctaatgatt cgtgtgcctg gtcatttttg
    12361 gcaaccatta gcagtccgga agtatctttg tcgattcggt gaacaatacc tggacgaata
    12421 acgccgttga tcgtagaaag atccttgata tggtacatca gcgcattgac cattgtgcca
    12481 tgcgtatgac ctgctgaagg atgaacgacc attccttgag gtttgtttat caccgctaca
    12541 tcttcatctt cgtaaacgat cgtcaacggg atattttctg ggaccacatc taatgtttct
    12601 ggttcgggga tcaagacagt aatctcgtct ccttgtttta ctttgtaatt tgctttgact
    12661 ggctgtccat tgacagaaac cgctccattt ttcagccaca gctgaatttg cgaacgactg
    12721 taatcggtga ataaattagt caatacttta tcgatcctac cagattcatg gtgaatcgtc
    12781 gttttaagtt ctgtcatttt tttctttgat ccttttcatc taaaataata taaattaaca
    12841 tacaaatcac tccacataca agtgtcacat ctgctacgtt aaaaatcgga aagctcataa
    12901 aatctgtctg gaacatatct actacatagc caagacgaat acggtcgata aaattgccaa
    12961 tcgcaccagc tagaatcaaa ctcaatccaa tcatgaacca gcgattacct ttttcacgat
    13021 ttttaatcat caatgtgaga atcacaacga ctgcaatgag tgtcactata gtaaagaacc
    13081 acatcttccc ttctagcata ctccatgcgg ctccagtgtt tcgaatatga ttcagtgaca
    13141 aaatacctgg tataagattt ttcgtctctc caagcgctag attatcagtg atccaccatt
    13201 tgaccaactg atccactcct actagcaaag cgctgaatat ccaataaata attaacaaaa
    13261 aatcaatcct tttctatttg tttataaata tcacgaggtc gtacgatccc gagcagatta
    13321 ccatcgggat tcccatcttt agtgataagt acggcttcca aagtcccttg ctcacgaaac
    13381 atcgcttcaa cctcgtaaat atgtgtttta gctggaacaa aatgataatt tgtgtacttt
    13441 ccgttttgga tgatcaaatc caacgctcgt ttatttgtta aatcaaccgg acctttttgg
    13501 ctttcttttg ctagccaaaa cccgatatta cgtaaagtaa tcaatgctac aaattttcct
    13561 ttattgtata acggaaattg tgaatacctt ttttctgcta ctatctttaa cagttcaagt
    13621 aacggaagat cttgttcaaa acctgttaca cgttttgcaa atcgaggcag cacggtttca
    13681 ggctgaatca gttcttgttc gatcagctgg attcgcttga ctgtccaatc atttggctct
    13741 gcaatgacaa aatcgactgc gatccgatca tggacgatcg cattccttaa ctgagcaagc
    13801 tgtaaaagat catcttcgta ttttctgatc attaattgtt ttctttgagc taggcgtctg
    13861 acaagttcgg taaatcccat atttcgtgca tttcccatct cgtctctcat ccatttttcg
    13921 attcgattga aactgattaa aaaaagatca gagtttgcca taattttcct ccttgatcaa
    13981 atatgcccgg ttttttatat agaaaaagaa cgcaatcaac acaataactg caaaaaaact
    14041 gaaaaataaa ttttgtagga aaacggcccc caacccagtt gctagaaata aaaacggata
    14101 gatttgaaga atcaagtaat tcattgtgat cacataagca ggctttgctt ctttcactgt
    14161 ataaacgggg ctttttagcc aataaaaggt gacctgcatc gtaaagggca cttccagatc
    14221 aagcgttatc ttttgttcat gactgagaac actgcgtttt tcattgttga ttcttagctg
    14281 aatcgagcta cccataccgt aaaaaccggt ttttcttata aatgtcgttt tcatcatacc
    14341 ctcctattat gctttagtat agctcaaaat acaataaaga aaaagaatga tcctagtgta
    14401 aacaaaatta attccctatc gtctaaaaaa agaagctgta gtcaaaatac agctccttcg
    14461 atggtagatt ataatcaagc ttaaaacaat cccaacactt ctccgtcatt tgtcacgtcc
    14521 atattcaatg cagcaggttt tttaggcaaa ccaggcatcg tcataacatc tccagtcaag
    14581 gctactaaaa atcctgcccc taattttggg atgatttcgc ggatcgtgat ggtgaaatct
    14641 tccggtgcac ctagcagtga aggatcatca gaaaatgaat actgtgtttt tgccatacaa
    14701 atcggcagtt tgtcccatcc attcttcgta aactcttcta tttgtttttg cgcttttttg
    14761 ctgaaaacaa caccatttcc tccgtaaatt ttcttgacga ttgtttctgt cttttcttgt
    14821 atcgtttctt ctaggcggta taatggcttg taattagctt cttttctatc aatcaatcgt
    14881 actacagctt ttgctaaatc aacgccgcct tctgctccat ctgcccatac actcgctctc
    14941 ttggcaagaa tcccctgatt ttcacacaag tgctccaaca gtgtgagttc attgtctgta
    15001 tctgttacga attcattgat tgcgacgatc acaggtagct gatattgttc catattacgt
    15061 atatggcgct ttaaatttgc aaaacctact ttcaaagcat caagattctc atttttaagt
    15121 tcatcttttt tcattccgcc atgcattttc aatgcacgga cagtggcgac aatcaccaca
    15181 gcatccggcg cttttttcaa gttaggtact ttgatatcta aaaatttctc tccgccaaga
    15241 tctgcaccaa atcctgcctc tgtgacaacg taatctgcta atctcaatgc tgtcttggta
    15301 gctaaaatac tattgcagcc atgagcgata ttggcaaacg gtcccccgtg gacaaaagct
    15361 ggtgttccgt aaattgtttg tacaagattc ggcttgattg catctttcag cagtaatgcc
    15421 agagcgcctt ctgctttcaa gtctccagca gtcacaggct gacggtcaaa tgtatagccg
    15481 acaacgatcc ttgcaagacg agcctttaaa tcttcaaggt ctgccgcaag acacaagata
    15541 gccatgattt cgctggcaac tgtgatatca aacccatctt ctcttggaac accttggatt
    15601 ggcccaccca agccaacaat tactttacgc agttcacgat cattgagatc gactacccgt
    15661 ttccaaatca ctcggcgggc atcgatgttc aattcatttc cttgatgaat atgattatct
    15721 aaaagagcag ataatgcatt attagctgtt gtgatagcat gcatgtcacc agtaaaatgc
    15781 aagttgatat cttccatagg caatacctgt gcatatcctc cgcctgcagc accacctttg
    15841 attcccatca caggaccaag tgagggttct cgcaatgcaa taacagattt tttattgatt
    15901 ttattgagcg cgtctcctaa ccctacagtg atcgttgact ttccttcgcc agctggtgtt
    15961 ggattgatgg aagtaactaa gatcaaatgc ccctcagcgt tatcctttga tcgattgatt
    16021 ccagaaaaat tgattttcgc cttatatttc ccatacagtt ccaaatcgtc ttccttaaga
    16081 tctaatttac tagccacttc aacaatcggt tctagctctg tttcttgtga aatttgcaga
    16141 tcattcttca tttgattacc ccttttcacg aactttattc aacaaaatat cttctttgtt
    16201 aagatatgcc aattatacct ttttttattc agaaaaaaaa cataaaatta caggttttta
    16261 atttatttga cgtacttata aatactcttt ataatgaagg ctatgatcag actttagaaa
    16321 ggtcgtgagt gctttgacca caggaattgt aaaatggttt gacaacaaaa aaggatacgg
    16381 ctttatcagt tatgatgaca cagaagaaat ttttgtacat tttactgcga ttgaagaaga
    16441 gggattcaaa actttggaag aaaatcaagt ggtcgaattt gagatcatcg aagggaatcg
    16501 aggaacacaa gctgcccatg tcaagaaagg caacgttcag actaccgata ctagtatgca
    16561 tgaatagata aacaaataga gtgagacgct aaacgggatg aaccgtacgt ctcactcttt
    16621 tcttttcctg taaaaattcg tttttttagg ttctgatttt taaaattagc tttttctttt
    16681 tttcgtataa ggataaggaa ttgattctga tttgggtccg tttttttcaa aatgatcaaa
    16741 aaggactact tgatcttttt ttcgcatcat gaaaacagat ggggctttct catcttgcaa
    16801 catcatttgc accccagtat tagttggcgt caaagatcga atctgtccga cagatatttc
    16861 tcggtctttc caaaaagcag cataacgtat aatcacagag tttgatttta aaataaaaga
    16921 acgccgcaaa cctagaaata gaaaaacaag aaaaatacct aaaacaaaat tgcttttcca
    16981 ataaggacct gtgttttcta aagaaagaat cagactaaaa aacagaatga tcaaggtcaa
    17041 tgaccaatag atgatcgtat gtgccagttc aggctgccat cgaatatttt ttttcattct
    17101 ctaacctctc accgattcag taatgatata atcttaccat aacattagtt tgaacaaaaa
    17161 ggagaatctc tttttatgtt aaaagtatac attgatgcct ctacaaaagg caaccctggt
    17221 cctagtggag gtggaatctt gattgtctat caaggtaaac aagaacagct atctatcccg
    17281 ctttctatag ggacaaacca tcaagcagag ttcgaagtct ttttaaaaac tttagaattt
    17341 ctaaagaaaa acgacttaca gaatgaaacg attttctgtt tttcagacag caaaacacta
    17401 gtttctacgg tcgacaaaaa cgcaacgaag aacgagctct tccttcccta ccttcaaaga
    17461 atccaagaac ttctatccga attttcttta ttgatcctgc aatggttgcc cgaaaacaaa
    17521 aataaaggag cagataatct tgcacgtcag gctctccata aacaattaaa agaaaaaaag
    17581 actctttaaa ttaaaaactt taaggagtct ttttatagtt tctcatttaa tataaaagat
    17641 aattgtgatc aaacgtaatt tccaattggt aatctccgat tttttcttct atttgactac
    17701 gtaagattct ttctagttct gtatcaacaa actggaattt ctcagggacc actacatcaa
    17761 attgcagcgt ttttccttga tgaattactc taaaatcatg aatattaagc cctgtacctg
    17821 tttcaagaag aatttgcttt aactgccgca agatattata gtatttttcg ttatcaacag
    17881 gcattggatc caagtgacag acaagttcca catccaaaga ctttttaaag tctttttcta
    17941 tcagatcaat aatcttatga gccacattca aattcattga ttcatctact tcgatgtgga
    18001 ctgatgcaaa gcgtttattg ggtccatagt tatgcaccaa taaatcatga tagccaagta
    18061 tatcatcgca agcatttaaa tgctcttcca tttcttcaat ctctgctggc gttgggcgac
    18121 ttcctaatag ttcgtataca aaatttcgaa gcatcgtcaa tccactataa aggatatata
    18181 atgctaaagc aaaacctata tatccatcaa tacgccatcc agttacccat tcgattgctg
    18241 ctgaaagcaa tacagccagt gtagtaaaga catcattata actatctgtt gctgtagctc
    18301 tcaatgtttc agaatcaatg gattgcgcaa taacctgata cattttgcct tgtgcaaatt
    18361 tcaaaaagat ggaaatgatt aggacaaaaa agacgatggg tgttaaagag acattttctg
    18421 gatggatgat tttttcagca gaagactcta aaaattgaaa tcctacataa gtaatgatga
    18481 tcgaaacaaa taacccactg atatattcga agcgttcgtg tccataagga tgttcctgat
    18541 ctgctggctt tgcagcaatt ttaaatccga tcaaagtcaa tatggatgaa gcagtatctg
    18601 atagactgtt cactgcatca gccataatgg agacacttcc tgaaaaaagc ccgatcatta
    18661 gcttagagac aaacaagatc agattcgtaa ccaatcctaa gatacccgct agtactcctt
    18721 tttttgtccg ctgttgcggt atttgtttct ttttttcttc ttgtttctta gccaagtttt
    18781 ttaccccttc tagtttgctt gttgctcagg taaaaagatg gagaggacga ttgttttcct
    18841 ctcctctctt cctttttcta tctatcgtgc gtcctattac tattaaaaga tggtcattat
    18901 tctggtttca acggtgttgc tgcaaataac gcttttgcta gtaatggtgt tatgattccc
    18961 gcacctattg cttctggtat cccattgatt gctgcaaccc ctgctaaggt tttaaataaa
    19021 agagcagagt ctacaccata ggcatctgcc gttgcacctg tataaaataa tcccatgaat
    19081 cctaacacta agactgtatt gaccaatgaa cccagtaccc ctgcaaaggc tgagctgatc
    19141 gttattgatt tatttttttg ataaatccag tggaaaacca agcctgctac taagccaaca
    19201 agtactctgg gaactaccga gataatcgga ttcgtgaaca ctaatgtatc aaatggcgtt
    19261 gttggcatgg cataggcacg aacaattgta gcgattcccc aaaacaagcc aatcaccatg
    19321 ccatcttttg ggcctaatac tactgcagca ataattactg tgatatggat aattgttaaa
    19381 ctaatgaatc ctaatggaat aaaccccagc caaggaacca tcgcttgtac gatgatgatt
    19441 gctaacaaga tcgcacgtaa gactagtcga aaggtcttat ttctactatt cattttttca
    19501 ccccttttta tttatactaa tcgtaaaaat ttctgtcact ttcgtcaact aaagaccatc
    19561 cgaatccgac catttgatta gatgcgccat ccaattgaaa aaagtatgag ggcaagactt
    19621 gtttgtcttc gcccccataa tcatgtttcg ttcaatgttt gaacttttgt ttttctgtca
    19681 aacgtaaacg ccctattgtt gcgcaaccca ttctttcgca gctttaagtg catctggaat
    19741 accagcagga tttttcccgc cagcttgtgc catgtctgga cgtccaccac cgccaccgcc
    19801 tactttcggt gcgattgctt taatcaagtc gccagctttt agtccagctt catttgattc
    19861 tttcgtcatt gccgctagca gactgacttt tccttcttgt tcgtttgcta aaacaagaat
    19921 atctgaaata tttttttgtt tccattggtc agctaattga cgcagctgat tcatatcttt
    19981 cacttttact tgtgctgcaa tgattgtttt tccattgatc gtttctggat ctttgaatac
    20041 atcaccagct tgttgattcg ctaacttgct agctaattgt tcattttcct tttgtagctc
    20101 tcttacttgc tgttgcaatt gttccgcttt tgaaacaact tctttcaatt gaggtgattt
    20161 aacaattgtt gctacttgtt ttagttgttt ttcttcttct tcaagtagtt cgtacgcttc
    20221 tttacttgtt acagcctcga ttcgtcgcac gcctgcacca attcctgatt cagaaacgat
    20281 tttgaagatt ccgatgtttt ctgtatttgc tacatgtgtg cctccgcaca gttcgattga
    20341 ccagccgcca atatttacta ctcgaacgtc atgtccgtat ttttcaccaa acaaagccat
    20401 tgcacccatg tttttggctg tatcgatatc tgtttcaatt gtttcaactg ggatagcttc
    20461 ccagattttt tcgttgacga ttctttccat ttcagctaat tcatctgaag tgatttgacc
    20521 aaagtgggta aagtcgaatc gtaaatgtcc aggagcgact aaagaaccag cttgatttgc
    20581 atgctcgcca agtacttctt tcaatgcctt gtgcagtaga tgggttgctg tatggttttt
    20641 gatgatccga ttgcgcatcg attgatcgat caatagatga taggtttgtc cttcactcat
    20701 tggtgctttt acttctacgg tgtgcataaa ttgaccatta ggcgcttttt gaacatctgt
    20761 aacaactgct acgatttcgc ctacttcatt catgatccat ccttgatccg ctacttgccc
    20821 acccatttct gcatagaaag gtgtttgatc aaagatcatt tgagcttcac cttctgcgac
    20881 ttcattcaaa agagactcac ctttaacgat gaccaataat tgactatctg attcttgttc
    20941 tgtgtatcca atgaatttac tttctacttt gatatctgtc aataaagcag attgtacccc
    21001 cattgatttt tcagtactgc gggcagagcg agcacgttcg cgttgtgcat ccatttcttt
    21061 ttcaaaacca gcatgatcaa ctttcagtcc ttcatcttct gccacctctt ccgttaattc
    21121 taccgggaag ccatatgtgt cgtatagttt gaagatgtct tttcctgcta gagtcgtccc
    21181 gttttcgttt tttacttctt ggatcacttg atttaagatt tccaaacctt cattgattgt
    21241 ttcattgaaa cgttcttctt ctgtacggat aactttttca ataaattctt tttgttgaag
    21301 tacttcagga taataactta ccatgatttc cccaacaaca gggactaaac gatataagaa
    21361 tgcttgttca atacctaatt ttttcccatg catgacagca cgacgaagca aacggcgtaa
    21421 gacataccct ctcccttcat tcgatggcaa tgcaccgtcg ccaatagcaa atgaaagggc
    21481 gcgaatatga tctgcgatta ctttgtaaga aatatctgtt actgggtttt caccatattt
    21541 tacttgtttg cttagctttt ctacggcgtg gataataggc ataaacaagt ctgtctcaaa
    21601 gttcgttgac gcatcttgaa cgattgagac catgcgttcc aatcccatcc ccgtatcaat
    21661 gtttttatga ggaagtggtt caaatgtgtt atcaggctta tggttgaatt ctgaaaatac
    21721 taagttccag atttctaaat aacgttcatt ttctcctcct ggatagtttt caggatcgtc
    21781 ttctgctaaa tcgttataag cttctccacg gtcatagaaa atttccgtat ccggaccaca
    21841 tgggcctgca ccgatatccc agaagttgtc ttctacatca ataatgtgat ctagtggcag
    21901 gccaacttct tcatgccaga tgcgttttgc ttctgtatct tttggataaa ctgttacata
    21961 aagtttttct ggatcgaatg ccatccattt tggatcagtc aaaaattccc atgcccagtg
    22021 gatggcttca tttttgaaat agtcaccaat cgaaaaatta cccagcatct caaacatcgt
    22081 atggtgccgt gctgttttac cgacattttc gatatcattt gttcgaatag atttttgggc
    22141 atttgtgatt cttggatttt ctggcacaac tgaaccatca aaatattttt tcaatgttgc
    22201 aacaccagag ttgatccaca ataaggttgg atcgttcacg gggatcaaag acgcactagg
    22261 ttcaatcgta tgtcctttag attggaagaa atcaagaaac atttgtcgaa cttgtgcact
    22321 acttaattgt ttcatgtcat tacctgcttt ctataaatta aatacaaaaa aacaccatca
    22381 cagccaagga cgaaaactcg cggtaccacc ttgattgcaa tgatgtgtaa tcattacctc
    22441 ttaaactcga taacgctgag ctgcgtcggt atttctacca aaatagaaaa agggagcaat
    22501 ttgattagtg actattcttt tttcagccaa gaaagaattt tctgtgagtc ttaaaaaaca
    22561 aatcatatcc ttttcgtact taattagtat acgcattcta ttgatcactg tcaatatctc
    22621 aatttttatt ttcatttcct tttggtcata ctccacgatt tttggtacga taagaataaa
    22681 ggaatcaata catctctgct attcatgaca aggaggaata tgatgaaaaa aatagttgga
    22741 gcggatgaat ggatacgcgc agctagctgc tttttgagat atcaagtttt tgtattagaa
    22801 caagggattc ctgcagaact agaattcgac ggaaaagata tcccgcaaca aacctattat
    22861 ctatttcttg atcaaaaaag accagtagct actttgcgtt atgaaaagaa aacacctact
    22921 tgcttcaagc cggaacgatt ttgcgtagca aaagaatacc gaggaaaagg gttaggcggt
    22981 cagttgcttg aagctgctga aaaacgggcg gtttcagaag gttgtcagac tagttatgta
    23041 gtagctgaaa tgacggctgt agatttttat gaaaaacaag ggtatcatac ttgcacagaa
    23101 ccttttattg aagatgaagt actttgtatc ggaatgaaaa aaagtctagc ttaaaaacaa
    23161 gagattgtga cacgcctttt gtcacaacct cttgtttttt atttatgtga atttttctta
    23221 tttttacgag cagtacggct ttgctgtctg cgttcgattt tgcgttcttt tttatttttt
    23281 tctgcaattg cccaatcgat ttttttctta taaccaggct tgatcttttt ctttttcttc
    23341 ttcacaagac caatcagcgt gggatctaat acatcttttg atttttctcg tttggcacgt
    23401 ctattacgat cgtatgtttt gacgatttcc ccgtttttga tttcttttgg ttcaaatgag
    23461 acgcccaatt tttctatcgc atcaatggct tgctcatcat ttggcgaata taacgtaatg
    23521 gccgttcctt ttaagccatt acgtccagta cgtccgacac gatggataaa gaagtctaga
    23581 tcttctggaa tttctgcatt aataacatga gagacacctt cgatatcgat ccctctagca
    23641 gctaaatcag tcgctacaac atattgatac tccagattct gtacttgtcg catcactcgt
    23701 tttctctctc ttggggtgat gtctccatga atctttgcta ctttcaagcc ttggtttttc
    23761 aagtaatcgg caatttcatc cacatgctgt tttgtattgg caaatacgat tgctaaataa
    23821 ggatgaccaa ttgtcagcag ttgataaatc atttgattct catctttacc ttttgttgaa
    23881 atcagccaat tttcaatgtc ttcagaaata actgcttttg gtttgatttc ttcaatgatt
    23941 ggattttcca tgtattttcg taagaatgga cgaagttttt ctggaatagt ggcagaaaaa
    24001 actaaaaact gcagcttttc tggtaatcga cttgcaattt ggtctacttc atttaaaaaa
    24061 cccatatcca atgtcatatc cgcttcatcg acaacaaatg caaatgcggt gtgaatcttc
    24121 agcgcttgtt cattcatcat gtctaatatt ctaccaggcg tgcctactac aacctgtggc
    24181 tgttggtgat gcagacgatt taattgacgc tgtttgtctg taccgccgac aaaattcgag
    24241 acacggatct ccggtgtaga aaatttagcg atttgtgtag ctgcttcata gatctgtgca
    24301 gctagttccc tacttggagc tgtaataaca acttgtactt cctctttttc tgcattgatt
    24361 ttttcaaaca aaggcaacaa aaacgtatgt gtttttcctg taccagtttg agattgccca
    24421 atgatatttt ttccttttgc aattaaagga atcaaacgtt cctggacctc tgtgggctgt
    24481 ttgaagccct ttgcggttaa cgcttcattg ataaacggtt tgaagcggaa tatatcaaaa
    24541 ttattcattc gtgcacctct tctctttaaa ttatcacgac tttggcatta taacacagac
    24601 aaagcgtttt tgctcagcta atttcgagaa tcatcatcct ttgtttggcc aatcgtgcta
    24661 acagtatatg tgtgactagc tttttcataa aacagaatcc gtttttttct caaaaatgat
    24721 agcaagatat ttaaaataaa aataccttgt agtaacaaga caccgagaga aatgaatact
    24781 gcactcatct gcaacaccgt attcgttgtc gaattcatga tttgctgaag caactgcccc
    24841 aaccagctta aaccataaaa aagaaagtac agatacccgt agcggatcat caatgctttt
    24901 aaagaaattc gttcgcggtc ttcttccacc aaacggatac gcacaatttt tttgccaagt
    24961 gtccatccat tggttataaa cggtacaatc ataaagtata cccctacttc taataaaagc
    25021 caccagatat gagaagtaac tgcccctgaa tcgaacgcaa ttttaaagaa aatcgccgct
    25081 accaaattaa taagagataa aaatgtccag tcgatcaaat aagcgaccaa gcgtcgagtc
    25141 aatgagacag tctttccttt ttcataagaa agttcatcca ttcgtcttct tgtaggaagc
    25201 ataaatgtaa ataacggcgt aacgacaaaa ccaatgattc cacccaatgt atttgtcact
    25261 aaatcattca catcaaataa gcggtatggt cttgggtaga taaaataaag tccacttagc
    25321 tgcgtcaatt caaaaaatag agaaaggcag aaactagcaa tcatcgtttt cataaaagaa
    25381 agccggaaat aatatcttaa ataaatgcca aacggtatga gtaataaaac attaaaagct
    25441 ggttccaaaa atacactctg cttcattgcc ggcaggtagg tactaggttc agacaagacc
    25501 aataccgtgt tgtctctgaa atgttgccat gaagcaaata aatgcagctc catctttggc
    25561 cctgtcattt gggcaaccgt ctcctttgcc ggaagtggta agatcaccaa aaaatacgca
    25621 cacaataaat aaaaaacgaa agaatacaaa ataactgccc ttgttactag aaatgttcca
    25681 tactttcgat attcataaat aaaaaaaaca gatgagatcg ctaaggctaa aaatggaaaa
    25741 acaatcagcg caaaacgaat cggttcgata taacttgcca tatgactcct ccttgattct
    25801 ttgtatctct cttatcttag cagtcctttg ctgtttcaag caagtaacga ggctattatt
    25861 tctcatttct ttcaaagtaa agtaagataa acacaaaggg ggtgtcgaga tgctgaatat
    25921 gtactttgtt tttggcgtac ctattttttt actgttcctt tatgctacga tcgcttatgt
    25981 aagaaaaaga actacaattc attatttagg atttattctc ctgattattt cgggtttcat
    26041 gctagtcttc aatcttcaga cttggcagca agcacttctt gaaatggata aaatgactcc
    26101 acatgctttg tctaaagtgc ttggctaccc tgtctatctg atttggcttc ccattttcat
    26161 ttctggttgc ttggttttat taaatattta tcgcggcgta cgccgaatag ttcagttacg
    26221 caaatcaaag taaaagacag aaatcggttg agcccgattt ctgtctttta ctttgctatt
    26281 tcatcgtaga caaaacgaca tttacgtgat caaaattaac gagtactttt gacatttctt
    26341 ccacgtactc ctcatttaaa taaaatattt tgtttggcag agaagtgcag ttctaataaa
    26401 attcgaatgt tttagctatc tacgttttaa atagagataa atcgggatac cagcaagtgt
    26461 caatactatc cccatcgaag ctaaaaaggt ctgagtgata agcgtgcttg atacgataaa
    26521 agtccctcca atgattgcta ctagtggaat aaacggatac attggaactt tgtatggtcg
    26581 aaacaaatca ggttcttttt tacgaagaag aatgacacca acaaagacca tcgtataaaa
    26641 aatccaaatg acaaagatca gcatatccgt tagtgtatca aaaccaccga tcatcatcat
    26701 gccaatagcg atgactagct ctaagatacc tgcggctaca ggaactttag ttttatcatg
    26761 aagtttagca aagagcttgc taaaaggcag gttgttttct tttgccataa cataagggag
    26821 acgcattcca gtaagggtat agccattgat ggttccataa acagaaatca agataccaac
    26881 tgtgaccagt cttccaccga agccgccaaa aatcattttt gcaacgtcac ttgccgcatt
    26941 gctgtttcct gcgactccgt cgatagatgc ggttcttaag aacaccgcat ttactaataa
    27001 ataaacgatc atgattccga tgattcctaa agaaattgct tttggcaaat ctttcgccgg
    27061 ctttttcaat tctcccgaaa tatttcctac gtgtatccaa ccgtcgtatg cgaacatcgt
    27121 tgctagcaag cccgctccca aggctgagaa aaatgaaaga ttttctcctg cttggatcgg
    27181 aaacagttgg aagtctactc cttcttgtcg gaacaaaccg aaaatgacga tcacaaacaa
    27241 aggaataagc ttgcagacaa gcgtaatcga ctgaaaagcc cctccagctt tagaacctaa
    27301 aaaattgatc aacaggatcg atacagcagc tgtaacagct acgggaacaa tcattgactg
    27361 ggaaagacca aatagattga cgaactgtgt accgaaaatg atcgacaagg ctgctacatt
    27421 tgctggaaaa taaatcacga cttgcgccca gcccaataaa aaagctgctg tatttccgta
    27481 aatccgttcg atatatttaa tcatccctcc tgtttctgga atagcagctg ccagttcagc
    27541 tcctgttagt cctgcacaga cagaaatcat accaccgaga aaccaagaaa acatatgcaa
    27601 actggcggat cctgtaactt ctgctacgct tgcggcttta aaaaataccc ctgcaccgat
    27661 cactgtgccc ataacagtcg ataaagcagt aaaaaatccc atcgtacgtt ttaattcatt
    27721 ttctattttt acttctttta ctggcgtggc ttgatattct tccatccctt gtcccctttc
    27781 ataccatagc caatcccaaa agtataaaaa actttttgga tgtggttctt gttttatacc
    27841 gtctctacca atttttcttt cagtttttcc aacatatcct ttgtcatcac atctaagtcg
    27901 tatttcggcg aaaagcccca ttcctcttgc gcacaagaaa catctagtga atttggccaa
    27961 gattctgcta ttgactgacg tacaggatcc acatcataat ccatttcaaa atctggtatg
    28021 attttttgaa tagatgcttt gatctcttcc gggtcaaaag acatcgccga tatattgaag
    28081 gcatttcgat gaaccaatcg atcaggatct gcattcatga gttttataat ggcatcgatc
    28141 gcatctggca tatacatcat atccattgat gtaccttgat caatgaaact agtgtacttt
    28201 ccttctttga ttgctgaata ataaatatcg actgcgtagt ctgtcgtacc gcctcctggc
    28261 aaggttgcat aagaaatcaa tccagggaaa cggacaccac gagtatctac gccatatttc
    28321 acataataat aatcacatag tagttctcca gctaccttcg ttaccccata catggttgta
    28381 ggacgctgaa gagtatcttg tggggtatga tcttttggag tatttggacc aaacgcgccg
    28441 attgaacttg gcgtaaaaaa tttcagctgg aattctctag cgacttccaa tgcattgacc
    28501 agcccagtca cgttcaaatt ccaggctaat tgtggttttg cttctgcaac agctgacaaa
    28561 agagcagcca aatgaatcaa tgtgtccact tgatattgtt ctgctatgct gcgcattttt
    28621 tcgtaatcca ttacatctag gatctcaaag atatcgtttt tctcaatgac ttcaggcatg
    28681 cgtatatctg tcgcaatgac attttcactg cctagttctt gacgtagccg tgtgatcaac
    28741 tctgaaccaa tttgtcctaa acatcctgtc accaatactt ttttcatgat gatcctccct
    28801 gatctttttt actactatct tcagatgatt aacaatatat gcgtgaacat gttctctaat
    28861 tttaaataga cgaaaaaact aaatttagtt aaataatttc catttctttt cctatttttt
    28921 gataaactgc tactgcttca tcaagcattt ccctcgtatg ttccgcagtc ggcatatttc
    28981 tgatcctgcc tgtcccaaga ggcacagttg gataaacgat tggtttagca taaatcccat
    29041 gttcgatcag cttcttagag aaagtctgag tcactttttc gtctcccaaa atgactggag
    29101 taataggtgt ttcagacata ccgatgttat agcctacttt tttcaattct tctttaaaat
    29161 aattagcatt ttcccaaact ttttcgacaa gttccggatg ttcttgcatc aaagtaatcg
    29221 aagccaatgc agctgcagct gcacctggag ttagcgaagt agaaaatagg aatggccgac
    29281 ttctagcttt caaccagtcg attagtgttt tcgatccagc tacataaccc ccaacaacac
    29341 ctatagcttt cgataacgtg cccatttgca tatcgatttt gtcgcttaat ccaaaatgct
    29401 tgacggttcc tgcacctttt ccagttacgc ctgagccgtg cgcatcatcg acgtaggtga
    29461 tcagtccata tttttcaaca atcggaataa tttccggcaa tcgtgcaatg tctccatcca
    29521 tggagaaaac accatctgtg atatacatga tttttttata tttcttactt tctatcgctt
    29581 cctttgcttt tttctcaagg tctttcatat cttgatgttt aattcggatg atttttgctc
    29641 cggataagcg gcaaccgtca ataatagatg catggtttaa ttcatccgat aagatagcat
    29701 cttcttttgt cattactgca gagatagcgc ccatattgca gttgaatcca gattgaaagg
    29761 cgattgctgc ttctgtccct ttgaattctg cgattttttc ttctaattgc tgatggattt
    29821 tcagcgaacc attgatcgtt cggactgctc ctgctcctac accataagtt tcggttgctt
    29881 caatacatgc ttttttcaat tcttcgcgat tggcaaatcc aagataattg ttggatgcta
    29941 gattgatcat cttttttccg tctacgatga tacaagcacc attttcactt tctagtatgt
    30001 cgatcgtatt gtataacccc ttttctttca gttgatccaa ttctttctct aaatacattg
    30061 ttagttcttt ggaagacatt gtctccacct ccatattcat ctgtcttgca gaacatacca
    30121 aacatatatc gtgtcattca tctacaaagc aagtatgcta tatttttgat aacgcttaca
    30181 ctgattatga gaattattta accacatttt ttatttacag tatcttttta ctttcaaatg
    30241 acttatatag tatgaacaat taccttttag ataatgatct attttactct acctgggcag
    30301 ataaatacaa cacaaccatt gatagaagcc ctttcgttaa aatcaattgt attctgaata
    30361 tttaatttat ttattgcata gaccaataaa aatacataaa atttccatct tcgtttatca
    30421 gcaattttta attttactct ctgaatattt cttaaaagtt ttacttgttg aattcgaaaa
    30481 aaggactgtg acaaaagtct tgtcacagtc ctattcttcc gaata
//
LOCUS       DBKOIJ010000017        28637 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000017 DBKOIJ010000000
VERSION     DBKOIJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.017"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            85..624
                     /locus_tag="ACV5NJ_000599"
     CDS             85..624
                     /locus_tag="ACV5NJ_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484722.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="ACV5NJ_000600"
     CDS             1644..2813
                     /locus_tag="ACV5NJ_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HHY6484723.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="ACV5NJ_000601"
     CDS             3286..3840
                     /locus_tag="ACV5NJ_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6484724.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4495)
                     /locus_tag="ACV5NJ_000602"
                     /pseudo
     CDS             complement(4013..4495)
                     /locus_tag="ACV5NJ_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            4498..5445
                     /locus_tag="ACV5NJ_000603"
                     /pseudo
     CDS             4498..5445
                     /locus_tag="ACV5NJ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            5716..6163
                     /locus_tag="ACV5NJ_000604"
                     /pseudo
     CDS             5716..6163
                     /locus_tag="ACV5NJ_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            6167..7000
                     /locus_tag="ACV5NJ_000605"
     CDS             6167..7000
                     /locus_tag="ACV5NJ_000605"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6484725.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            7245..8270
                     /locus_tag="ACV5NJ_000606"
     CDS             7245..8270
                     /locus_tag="ACV5NJ_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6484726.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8408..8533
                     /locus_tag="ACV5NJ_000607"
     CDS             8408..8533
                     /locus_tag="ACV5NJ_000607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484727.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            8558..9673
                     /locus_tag="ACV5NJ_000608"
                     /pseudo
     CDS             8558..9673
                     /locus_tag="ACV5NJ_000608"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            9756..10652
                     /locus_tag="ACV5NJ_000609"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="ACV5NJ_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            10668..11492
                     /locus_tag="ACV5NJ_000610"
     CDS             10668..11492
                     /locus_tag="ACV5NJ_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6484728.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="ACV5NJ_000611"
     CDS             11524..12834
                     /locus_tag="ACV5NJ_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HHY6484729.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="ACV5NJ_000612"
     CDS             12982..14241
                     /locus_tag="ACV5NJ_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HHY6484730.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="ACV5NJ_000613"
     CDS             14251..15123
                     /locus_tag="ACV5NJ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6484731.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="ACV5NJ_000614"
     CDS             15135..15965
                     /locus_tag="ACV5NJ_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6484732.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="ACV5NJ_000615"
     CDS             16005..18188
                     /locus_tag="ACV5NJ_000615"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HHY6484733.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="ACV5NJ_000616"
     CDS             18249..19469
                     /locus_tag="ACV5NJ_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HHY6484734.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="ACV5NJ_000617"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="ACV5NJ_000617"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HHY6484735.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="ACV5NJ_000618"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="ACV5NJ_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123848.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="ACV5NJ_000619"
     CDS             22431..22640
                     /locus_tag="ACV5NJ_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HHY6484736.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="ACV5NJ_000620"
     CDS             22633..22782
                     /locus_tag="ACV5NJ_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HHY6484737.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="ACV5NJ_000621"
     CDS             22936..23601
                     /locus_tag="ACV5NJ_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6484738.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACV5NJ_000622"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="ACV5NJ_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HHY6484739.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="ACV5NJ_000623"
     CDS             25304..25537
                     /locus_tag="ACV5NJ_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HHY6484740.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="ACV5NJ_000624"
     CDS             25534..25941
                     /locus_tag="ACV5NJ_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HHY6484741.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="ACV5NJ_000625"
     CDS             complement(27078..27596)
                     /locus_tag="ACV5NJ_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HHY6484742.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27963..28211
                     /locus_tag="ACV5NJ_000626"
     CDS             27963..28211
                     /locus_tag="ACV5NJ_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484743.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9843 a         4314 c         5245 g         9235 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagc
//
LOCUS       DBKOIJ010000018        27915 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000018 DBKOIJ010000000
VERSION     DBKOIJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.018"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(190..1134)
                     /locus_tag="ACV5NJ_000627"
     CDS             complement(190..1134)
                     /locus_tag="ACV5NJ_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHY6484744.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1354..2568
                     /locus_tag="ACV5NJ_000628"
     CDS             1354..2568
                     /locus_tag="ACV5NJ_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6484745.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2590..4605
                     /locus_tag="ACV5NJ_000629"
     CDS             2590..4605
                     /locus_tag="ACV5NJ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HHY6484746.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4688..6061)
                     /locus_tag="ACV5NJ_000630"
     CDS             complement(4688..6061)
                     /locus_tag="ACV5NJ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HHY6484747.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(6087..6371)
                     /locus_tag="ACV5NJ_000631"
     CDS             complement(6087..6371)
                     /locus_tag="ACV5NJ_000631"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6484748.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6373..8388)
                     /locus_tag="ACV5NJ_000632"
     CDS             complement(6373..8388)
                     /locus_tag="ACV5NJ_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HHY6484749.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8567..8872)
                     /locus_tag="ACV5NJ_000633"
     CDS             complement(8567..8872)
                     /locus_tag="ACV5NJ_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HHY6484750.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8961..9991)
                     /locus_tag="ACV5NJ_000634"
                     /pseudo
     CDS             complement(8961..9991)
                     /locus_tag="ACV5NJ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            10198..11073
                     /locus_tag="ACV5NJ_000635"
     CDS             10198..11073
                     /locus_tag="ACV5NJ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HHY6484751.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11225..12067)
                     /locus_tag="ACV5NJ_000636"
     CDS             complement(11225..12067)
                     /locus_tag="ACV5NJ_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HHY6484752.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12159..14036)
                     /locus_tag="ACV5NJ_000637"
     CDS             complement(12159..14036)
                     /locus_tag="ACV5NJ_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HHY6484753.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(14033..15454)
                     /locus_tag="ACV5NJ_000638"
     CDS             complement(14033..15454)
                     /locus_tag="ACV5NJ_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HHY6484754.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15457..18846)
                     /locus_tag="ACV5NJ_000639"
     CDS             complement(15457..18846)
                     /locus_tag="ACV5NJ_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HHY6484755.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            19103..20494
                     /locus_tag="ACV5NJ_000640"
     CDS             19103..20494
                     /locus_tag="ACV5NJ_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6484756.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20623..21897)
                     /locus_tag="ACV5NJ_000641"
     CDS             complement(20623..21897)
                     /locus_tag="ACV5NJ_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HHY6484757.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(22076..22723)
                     /locus_tag="ACV5NJ_000642"
     CDS             complement(22076..22723)
                     /locus_tag="ACV5NJ_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6484758.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22731..23417)
                     /locus_tag="ACV5NJ_000643"
     CDS             complement(22731..23417)
                     /locus_tag="ACV5NJ_000643"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484759.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23423..24496)
                     /locus_tag="ACV5NJ_000644"
     CDS             complement(23423..24496)
                     /locus_tag="ACV5NJ_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484760.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24718..25392
                     /locus_tag="ACV5NJ_000645"
     CDS             24718..25392
                     /locus_tag="ACV5NJ_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHY6484761.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25397..26737
                     /locus_tag="ACV5NJ_000646"
     CDS             25397..26737
                     /locus_tag="ACV5NJ_000646"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHY6484762.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26886..27872)
                     /locus_tag="ACV5NJ_000647"
     CDS             complement(26886..27872)
                     /locus_tag="ACV5NJ_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HHY6484763.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8125 a         5269 c         5150 g         9371 t
ORIGIN      
        1 tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat
       61 cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa
      121 cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag
      181 attttcttct taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa
      241 taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga
      301 aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc
      361 ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc
      421 aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa
      481 tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa
      541 taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg
      601 actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt
      661 ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat
      721 caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa
      781 ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct
      841 ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc
      901 attaataatt tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt
      961 atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa
     1021 tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt
     1081 gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaat
     1141 gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc tttcgcttat
     1201 tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa agactttcta
     1261 tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact tcatactata
     1321 tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa
     1381 ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac gtctgcaatt
     1441 cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt attaacgact
     1501 gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc taggaaaatc
     1561 ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg tatcctcccc
     1621 ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg tattggcttt
     1681 ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg agatgaacga
     1741 gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt aatgacattt
     1801 gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta cgccatcagc
     1861 ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac tgaacttcaa
     1921 tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat gattagttat
     1981 ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa attagccaca
     2041 gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt aacaatcatg
     2101 caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa agcacatcgc
     2161 tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat tgccactgtg
     2221 ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt ttcgttattc
     2281 attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga gagtttcaat
     2341 acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc
     2401 tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc
     2461 ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca
     2521 gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg
     2581 tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt
     2641 gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca
     2701 gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt
     2761 gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga
     2821 caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc
     2881 tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca
     2941 gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt
     3001 tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca
     3061 gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc
     3121 tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag
     3181 tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga
     3241 aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga
     3301 atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca
     3361 agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt caagttatgt
     3421 agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg
     3481 tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg
     3541 atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat
     3601 tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt
     3661 ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat
     3721 gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg
     3781 gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg
     3841 atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat
     3901 ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa
     3961 gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg
     4021 aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag
     4081 aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa attctatgaa
     4141 caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat
     4201 tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa
     4261 acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca
     4321 tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag
     4381 ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt
     4441 acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt
     4501 acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca
     4561 aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt
     4621 gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga
     4681 agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc
     4741 tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt
     4801 attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag
     4861 aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca
     4921 ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga
     4981 agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag
     5041 tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc
     5101 caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag
     5161 aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca
     5221 ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg
     5281 caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta
     5341 acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc
     5401 gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa
     5461 aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa
     5521 cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta
     5581 atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg
     5641 cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa
     5701 aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca
     5761 tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc
     5821 taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg
     5881 aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag
     5941 ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca
     6001 aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca
     6061 ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct
     6121 tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa
     6181 gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca
     6241 cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt
     6301 gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg acatactgca
     6361 aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga
     6421 tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa
     6481 agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg
     6541 ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc
     6601 gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa
     6661 cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt
     6721 ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc
     6781 cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt
     6841 cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac
     6901 gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat
     6961 tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa
     7021 gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg
     7081 tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga
     7141 attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa
     7201 tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc
     7261 tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc
     7321 agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta
     7381 gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata
     7441 ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa
     7501 cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc
     7561 cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag
     7621 agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg
     7681 tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat
     7741 ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg
     7801 atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt
     7861 ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag
     7921 tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc
     7981 tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc
     8041 atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg
     8101 aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct
     8161 tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca
     8221 gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg
     8281 agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag
     8341 actgatttct cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac
     8401 ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta
     8461 cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa
     8521 gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat
     8581 gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga
     8641 tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac
     8701 cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt
     8761 ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag
     8821 gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc
     8881 ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa
     8941 acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct
     9001 tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca
     9061 ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt
     9121 actttttctt catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg
     9181 ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct
     9241 tgtcgatctt ttgtttgttt cacgaacatc ccttttttcc gtaaacgacg aaacttggac
     9301 gttctttgat gcggaaataa ctggatttta tcgaaatttt catatttcca tagaaaaaat
     9361 ccagatcgaa gtaatcattc attcgtcctt tccctaaaag ttgacgtaca tcatagctga
     9421 tatcatctgg ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa gcatgtccat
     9481 ataaatagct gttcgcagta gaaaattcat gcgtattttc tggtacttcc ggtctgaaat
     9541 aatcataatg catttctact tctaaaatat cccctaattt tccgctttcg attacttttt
     9601 ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta acattggata aatagacctt
     9661 tttcttttgc taatgcaaat aactcttctg cttcctttga cgtttctgca aaaggtttct
     9721 ctactagtac attcttacca tgttcgagtg ccatttttcc atattcataa tgaaattgac
     9781 taggtgtagt gatgacaact aattggatct caggatcatc ccaaagatca gacagctgat
     9841 cagtatattc gatgtcttcg aatgttttcc acactggttc agatggggaa aaaatttttt
     9901 tgatattgat tttgttcgta agttttcttg aaaaaggcaa atgatagcga tttgtactct
     9961 ttccattgcc aagataagcg attgtaagca tgagtggtgc ctccttttat tgatgttttc
    10021 agtatatcct agaaaagcgc agaaagtatt tataatagct attctggtaa aaaagttcag
    10081 aaaaacactt gttatcattc ggtttggtga aattaatgtt caaagtaagg ataaaaatca
    10141 aaaagatagt agcggaacca agtgtttatc agtaagatag aatttggagg tcagatcatg
    10201 cttattgaag aaaaactaac aaaacaagag ttgtttacca cgacagagaa acggattgtc
    10261 gattacatcc gtaggaatat cgaagctgca gtctacatga cgattgaaga attagcaaaa
    10321 gctacgtata cttcccattc agctatcatt cgtttatgca aaaaaatggg attcagcgga
    10381 tttaaagaat tccgtttcga gctggggcgg gaagttcatc aattgattgc tcagttcaat
    10441 caaacgattg atcctaattt tcctttttca agtgaagata gcactcagca aattgcaaag
    10501 aaaatggcgg atttatcgat ccagtcaata aaaaaagcac aattgcagat tgaacatgaa
    10561 gatttagatc aaatcggtaa agtattgaac aaggcacagc gaatctttct ttttgcaaaa
    10621 ggtgattctc aaatcacggc aaggaaattc cagaataaaa tggtcaagtt gaataagttt
    10681 ttgatcatgg ctgaagaata cagtgattct tcttggaatg cagccaattt gactagcgag
    10741 gattgcgcca tttttattag ctacagcggt cgcatccatc attatgaacg aataatgacc
    10801 tatttgaagc atgtagaggc ccctaccttg ctgattaccg gaaatcagca ttcagaaatg
    10861 gccaaacagg ctgatatgtg tcttgttata tcgcaggagg aatatgattt tgctaaagta
    10921 gcgactttct cctctcaaat tgccttcgat tacgtattaa atacgttatt ctcagtgata
    10981 tattctcaaa attttgaaga gaatatccgc aacttgaaaa acaaacaaga attgatgcaa
    11041 aaaggccttc ttaaagaata tcggaaaact taagaaaaga tcctcattta gtaataagaa
    11101 agatagtgtt tgataggctc tctttgatat catcataaaa acaggctgaa gttttctttt
    11161 tcaaacctca gcctgtttgt ttacggaacg attcgaatag atatattata gaaatgagga
    11221 atagctaact tctgtttctt attttgaacg attgacgaaa aagatatata agaaggagga
    11281 aaatcagtat acatgcagct aacaatccat acaggaaaaa tcgtttgttc tcggatactt
    11341 tctgtacttc tttttgaact tcttttgcat gataaggaac ccggtgccct ctgacaagta
    11401 agcgatgact gttgaccata taaggtgtac aagttaggag tgtcacgaaa tcctgccctt
    11461 tttcgattct cagatctttt gtatcagtcg gttctaccgt cttgatctga tcgacttgat
    11521 aagcgagtgt ccgtccattg atttcgatat agaaagaatc acctaaagtc agtttcggta
    11581 agtctgtgaa caactttgct tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat
    11641 tttctccccc cgtaggataa gacgttcctt ccaataatga ggcacctttt tctagcagca
    11701 cagtattcgt ttcatcaaaa attggcaaat ggacattgat ctcagggatc gtcaaaatac
    11761 cgatcgtatg tttctcaaaa taggttctat cctttttcgc aggttctttt ttcttagtaa
    11821 agggatctgc acctggattt ccaccttctt ttgctaattg ctggtttttc tttgtcatgt
    11881 tttcttggat tttagccatg acttcttcat tttctttgac cgcttgttgc tgataatgag
    11941 agatgatctg ctgatccaga taattattca gcgcgtcgct aacaaatgga taagctagta
    12001 tccctattcc tgagagaagc aagaaaagca tcagcagatc tagaagtttc cttttctttg
    12061 gtttcatttg tgttctcctt gtgatgactg tttgataata aaagaaaaag gagcatttgc
    12121 gtaatccgct gctccttttt ccaattcttg caaaaagact aaatctgact gtgcttgcgt
    12181 ctagcaaagt acagtccagc aatcagtaga aggactactc ctgcaccgat atacacatag
    12241 attcccttac cgcctgttga aggaagtgta cctttgtgtt tatttggtat tttttcagga
    12301 gaaatcaact gtcctgctgt tacataagat tgttcatcga tagtaaatgc tacacggttt
    12361 gttaatggaa catatttttc tggcgctttc gtttcttcca gatagtacgt gccatatttt
    12421 agacctgtca catcgattaa accgttactt gtggttgtaa aaaccgttgc tgattctttc
    12481 gccgataccc agctgacggc ttttgtggat gggtcgatcg ataaatattt cgctgtgtca
    12541 ctatcttgat cacgaacgac gaattctgct ccagcaagtg tttggtctga tgtaacgtca
    12601 ccatctactt taacaaatcg tttgccccca gtaacgacat cgactgacgg tggtgtttga
    12661 tcatttgtat gcccgttatc gacatttgct tggttagaaa atcctttggt tggatctgct
    12721 ttttcgttca aatgcatata gtaaacgaat ttcaatgtac cgccaggagt taatgaagga
    12781 atataattcg gatcaactga aaccgtgaat ccgtctgttt gctcagtgac agaataattt
    12841 actgggtcga tttctttatt tccatcatat aaggcataag catacgttcc tgaagaatca
    12901 ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt gtacttgttt
    12961 tgcgttcctt ctttatcagc gatacctaat gggatattga cagaaatctc atattgaatt
    13021 ttttcaccga ttgcgacatc ttttccattc aattgaggtg ttgttttatc aactttagac
    13081 gtatcatttt tgatggtctt ttctactggt gtttggctat ttgccaggat ctcaaaaggc
    13141 gtgattgttt gcttttcaat catttccgca ttatcaggag cttttacttc ttctaaatta
    13201 tattttccaa cttctaaatg attaacgatc aactggcctt tttcaataga tgcctcggca
    13261 aagtcattgt tgccgatgtc ataagaatga ccagtaatga aatgtttggc tttggtttga
    13321 tcagttgtcc aagtgtacaa tccatctgtg acactttgga tgtatttttt gacgcttggt
    13381 gttccttctt ctttagaaat aataaattct gctccattta gtgcttcgtt ttcggcagta
    13441 ccgatttttg taactttcaa cgttccatca ttaccgactg tatttttagg gtagagatgg
    13501 atagtatcta gttcttctgt cccgtattta taagagccat ctgcttgttt gatcatctca
    13561 tatacaggga aagctaaaac catgtttgcg gcagctgaca ctccgtcttt tggttcttct
    13621 ttgatcgtgt agactgcatc tttcccattt tgttttttag gtaaagataa agtgacattt
    13681 ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga ctggactgct
    13741 tgttttgctg catccacgga cgctccttta tctcgttgcg cataaaattc ttgagtgacg
    13801 ttataaactg aaaatgaaat atcggctaat ccttggtatt gatcgaattc gctcatttct
    13861 ttcccgctgt tttggattaa aggatcgggt aaatcagtca ttttcttttt atgaaggatg
    13921 acttgagctg ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat
    13981 ggtaaaataa ggaaaaggac tcctaacata acgtttattt ttttatgatt tttcattttt
    14041 tcactcctgt tctttttttg ataaacaacc agattgcaaa gcttgcgaat accataccaa
    14101 caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt
    14161 cttttttatt gactactttc aatatactgt ccgcctcttt atcctcagaa agaaaagttg
    14221 aaatggtaaa cggaatttct gtttcactta aaatataacc agaaggtgct tcgatctctt
    14281 ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa
    14341 tcaggtgaag cgcatctgat tcgttctttg tccacttgta tccgttggct gtttgctgga
    14401 ggtaatttcc tgctttgttt ttaataagga atactgcgcc agctaatcgt ttatctggtt
    14461 tatttgtctc gattttgaca aattgcttac tccctgtata tacagagact ttctttgtac
    14521 tgacaccaaa accaaaatcc agtgttccgt tgttgttgat ttccttgttc gcctgtgctg
    14581 tactgcttag ctgcatctga taagaaatgg tcagtttttt tccagcatat ttttccaacg
    14641 aagggatcga aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca aagccttctg
    14701 tcagcttttc tccattcgat gaaatcgtta aactattagg taaaaacgtt aatgcttcat
    14761 ccgcactatc tgacaattcg aacttttgat agtcaaggat atttacagga attgtcgtat
    14821 gcaaggaata agtgattttt tcaccaatcg taaaatcatt tctaggctct tctaatacct
    14881 tctcaaaagg tggatcaggg tagtcgtttt cttcattttt aggatacaaa tggatagttg
    14941 aaagtttttg attgttttgt ccatgtacag gaagaacaac taccatattc tctgcctttt
    15001 ctttgacgac ttctggtact ttggattcaa tgaaaagata cgctttatct cttttttctt
    15061 gatctttagc ggccagagaa aaagaagcga taccatcttc gttgttaaga gttgtagttg
    15121 ttgcttctgc ggtaaacata ccggaagacg caccgttttt tgcgatctct gcttgtgctt
    15181 cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca tcataaactt
    15241 ggaatgtgac accatttaat cctcgatacg tttgtaataa agctttttct tgtccgtcat
    15301 ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa
    15361 ccatttctgt ttctgtttct gttgctacct gagtaaaagc tggtttaaat agtaacaaga
    15421 agagacacat actaagccaa ccaagttttt tcatatttag ctgccccctc tttctttttt
    15481 caaaaaaaga taccaaatag aaaacgcaca accaatcatc cctactagat agattcctaa
    15541 acggccaatt cctcccgttt caggtaatgg tacttttgcc tgattcgtga tgtctaaaga
    15601 aatctggttg tttttggctc ctggtgacag aacagattca tgatcttgtc catccacttg
    15661 aattgaccca tcttcgtgta taactatagt aactggctct tttagacctt ggtatccttc
    15721 tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt aaattttcga atagaaaggt
    15781 atctgtttct tgtccatctt ttggcgattc taagtccatt tctggtccct gcagtctgaa
    15841 ttttgctcct tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc
    15901 ttttagtcga ttttgatgag tgagcgtcca tacattattt gtcatctcgg tattttttcc
    15961 cgtgtaaatg atttttccat atttatcgat ttggaattca taatttcccg gcgaagtatc
    16021 atatccggca ggaccagctg tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa
    16081 actggcaaga cctttttcat tcgttgtttg gctgtcaatt ggctgataag taccatttgc
    16141 ttcttttttc tgtagggaaa atgctgctcc tttaagattt atctctttgt ccgtcccttg
    16201 catcgcatac ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg
    16261 gatcgtatca tcggaagtag tgactgtttt tccatcaatg gttaccgtac catcagaagc
    16321 gatcttgatt tcccaagtag tctttttgct taatccatgc ccttctggag cttttgtttc
    16381 tgtcagcgta taggtcattt ctttttgcaa tttgacattt tctggaagag aataggttcc
    16441 gtcgccatga tctgttagta aagtatcaat agaatctcct gttaatttga aaacagcgcc
    16501 aataagatcc ttttcacctg tagaggaatt tttcgttatt ttcaagttta acggtgtgaa
    16561 ttgtttagta tttttccatg tcattgcatc tatttgttga gaatcgtatc ctggtacagg
    16621 taattctttg attgtttggt aactgaatgc ttgaccttga ttattgtatt gaggtagtga
    16681 taatatctct tgataacttt ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc
    16741 ccacgtattt gttgtatctt tagctggttt aatgattcga atatatccgt ttttccaagc
    16801 tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg gacgatcagc
    16861 tagattgttg tcaaattctt cccataactt tttgatgtga agactgactc cgggagcttt
    16921 agcagatggt ataccgaatt cagctaattc attggtatca atgtttggct ggaaagttgt
    16981 cctgccgttc atttgatacc agaaatttgg atggaagtcc tcattttctg tttggattct
    17041 cacttggtaa tggatttgga tttcttggtc ttttcctaaa tagatctgat tgctcttgat
    17101 ggtatttcct tctatggaaa tagatggagt gaccgttgta ggacttgtcc ccacactctt
    17161 gactgaaagt gtaccaggct gataaatgaa tggttctgcg attggatcat ttatttgtcc
    17221 attgcttaca gttgctgaga tctgtacagc ttttttggct aggtattcag agatatctgt
    17281 tgcatgatca gctgattcat agtaaagatc gcctttttca tctgatgaaa ccatttgtcg
    17341 catacgagac tctacttctg cttttgagag accagctgcc ggatcgcttt gaagttggat
    17401 gccaagacca tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc
    17461 gataaacgta ctgtcgattc ttctggatag attgttttgg tcaggtgcat catagattct
    17521 tgagattaga gaagtatttc ccggccgatc ttgcgtatta gaaaactgag ttccgtaata
    17581 attgctgctt gattgtgcgt gtacccgctg tactttatag gaaaatgttg gtacaccatc
    17641 cgtcagcaaa acgatcactt ttttatgtcc atttggaacg gatagcatgc ttcctgcatc
    17701 tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa tggattttac
    17761 ttgatttttc actgaatcaa atgaacccat ctgtactgca ccgttactgt agctatatcc
    17821 ttcgcttgag tagccgacat atcccatatt gattttgtct gtgataccgc tatctgctaa
    17881 agtatcgaca aaacgatcga caccaatctt tacttcaccg atccgattat tgtcgttcat
    17941 acttcctgac cagtctacga ccaatacgag atcaagaggc gtgatatctt tttgtacatt
    18001 tccacgagca ttcaaataaa catcaaacaa tccgggtgta cttgtttcct ttgcatactt
    18061 tcgtatcgca tagtctgctt gattaccggt tcctccatat tcgatatacg aatgggtccg
    18121 atcattagga tctccattcc aagaatttat accatcccat tgatttgttc cgtttttatt
    18181 gccttgatga tcaagaacat ttgtatttcc tgtaggttgc caactggctt taggataagt
    18241 gccctgatca tctgttgtat attctgtagg aatcgtttga ttagatggca cactcacagc
    18301 tggcaaggat aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt cttttgatga
    18361 ttcttcttct atttcgtctg gctttggtac tgacacatct gtatctttct tattttctgt
    18421 ctcatcaata atgtcatatt ctttattatc gacgatcagc ttgtttgcct ggctgtggta
    18481 aagtgctgga tcgattggaa aactcagatc gatagtttgt ttagtgtttg atgagatttc
    18541 caacgtaatt ttcccgctat ttacctgata agcatagcca tctccttctt ttctttccac
    18601 atcacttaag taattttcga ctagaccaat ttctttttct atcttttcag cactggtgtt
    18661 attcgcattt agcttgatgt tcagtgcttt ttttccagac tgatccgaaa tgacttcagc
    18721 tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct gctattgcag ataatggcac
    18781 aaagtttgat aaactcaata ttatcagcat gaaaataact ttcagtttct tccctgtggt
    18841 cgtcattttt cccctccttt ttcttttttt ctgttttgac gtgaatagtg aaaattatta
    18901 aaataaacag gcgttacata attttattta ttttaatatg ataattatta tacgaagggc
    18961 gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt
    19021 cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt
    19081 gttggaatta aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa
    19141 gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag
    19201 gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa
    19261 attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat
    19321 gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac
    19381 gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa
    19441 acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca
    19501 gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag
    19561 ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat
    19621 ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt
    19681 ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg
    19741 gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt
    19801 gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt
    19861 caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg
    19921 aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata
    19981 tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac
    20041 tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct
    20101 atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat
    20161 ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta
    20221 tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat
    20281 tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat
    20341 tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca
    20401 acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa
    20461 tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa
    20521 ccaccctgat aaaaatggct cttcgtcaaa ttgtgttggc gaagacaaat ttgaataaaa
    20581 cttagtccga tatgcggcgc gttctgtgcg ccgcatattt ttttagtttt caaagatcag
    20641 accttgttgt aaatagatcc gtgcccgaaa gttataaaag ttgcgataac caaacgcaat
    20701 tcgtttaatc agcttgatct tgttattcag cccctcagtg acgccgtttg agtggggcac
    20761 cttaaaggca tttgaaatac ccgattggta gcgatggaag atcgtgaatt tctttttaaa
    20821 cgactgaggt aacgtagatg aaattgtttt aagggtttct tgaaagcctt ccgtatctcg
    20881 tttaacgaaa taatagcgta ataattgaca aaaatgatac gcagttcgca attcttcgtt
    20941 gtagcttaac agctcatcaa cgatttctgt ttgactaatt ggtcgtttaa acagtcgatg
    21001 ataatgccgt ttgagtggct ccaatgtcgt ggaatctttg agtaagagtt tccaatatcg
    21061 cttgagccga cggtacttct tctgattttc agagtgtgag gttcgaaaac gattcatgat
    21121 ctggacacgt aattgattga agctgcgatt gatgtgttgg acaatatgaa agcggtcagt
    21181 tacgatctcc gcatttggaa agactgtttt aagcaactga caatagctgg cattcatgtc
    21241 catcactagg tacttgacct ttaaacgggc ctttctagga aagcgcagga agtattgagt
    21301 tagtttgtaa agtctgcgat cttcaaggat cgtaaatagc tgattggttg tcccatcaac
    21361 acaaataaaa ctcatggaac cagaaacaga cttcattgat ttgaactcat ccatacacaa
    21421 caccgtcgga aggaagttca tacgtggctg gtaatgcttc gttgcttcac gcaagatccg
    21481 tgtcaccgtc ccatccgaga cgaagtactt cttagcgatt agtttacgtg attgattttc
    21541 gtacagttcc atgagaatcg cctgcttcag ttcctttgtt agttgatgat gttcatcaac
    21601 taagtccgtt ttcgcagaaa aggtccgtcc acaatcatgg cagagaaaac gggtacgttt
    21661 taattctaaa atagtcaact tttctttgac tttcaacatc tgagtgcgtg tcgtatagga
    21721 accgttgcgg ataatacgaa tcgatccaca gtgttcacaa tgggaaggtg agtcatccaa
    21781 tctcccttta ataaactgcg cttgaacgcc tttatattct ttttcaatca gccattcttc
    21841 atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt ctagtatgat tatccattga
    21901 gagactcctt tggtagggtg acgttttggt cgactaaatt ttaccaaaag tgagtctctt
    21961 tttgagttga aacgaagaaa aaagtgttag tagaaaatcg ctttcgattt tccaccaaca
    22021 caaaatatta tagaaccata aaaatttcag gacaaataca catagctgtt tctttttatt
    22081 gtattccttc agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg
    22141 tccaattatt tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata
    22201 ggaaactaac tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca
    22261 gccatttttc gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat
    22321 catttcgctg ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc
    22381 agcaccactc taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga
    22441 attttaagat atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat
    22501 cttccaaatc ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga
    22561 tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat
    22621 cgattctctg tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct
    22681 ctcctttatc tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc
    22741 tttttctctt cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga
    22801 atcatacgga catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta
    22861 actagtaatt tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct
    22921 gctaagatca agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct
    22981 ccaccagaaa ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc
    23041 aaggaatcga tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat
    23101 tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct
    23161 ttaaagcgta aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc
    23221 actatttctc cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta
    23281 cctgaaccag aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt
    23341 gctttcaaag ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc
    23401 gttaatattt ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta
    23461 tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata
    23521 gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca
    23581 aataagcaaa caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta
    23641 ctaaagattt agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag
    23701 cagtcttctg aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga
    23761 acaaaaagta tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt
    23821 caatgaatgt cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt
    23881 tagctgaatc ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct
    23941 gttgtccata cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg
    24001 tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt
    24061 tgatcgtatc tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat
    24121 tctttgtttt aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg
    24181 tgccgaaaac tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga
    24241 ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg
    24301 caaatacttg atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg
    24361 cttttttaaa ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg
    24421 caatcaagaa cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt
    24481 tgatagctag aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac
    24541 ttgttgaata gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca
    24601 ataaattgtc taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta
    24661 agccttattt atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg
    24721 attaaactat tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc
    24781 gctgcaatcg gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa
    24841 agtggagttt atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa
    24901 gtgttagcaa aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa
    24961 gatagtttgg aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa
    25021 cctttctatc gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt
    25081 ctctttgatg aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa
    25141 gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat
    25201 tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga
    25261 tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat
    25321 ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg
    25381 caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc
    25441 gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat
    25501 attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc
    25561 tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac
    25621 tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg
    25681 ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat
    25741 tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat
    25801 ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac
    25861 gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa
    25921 tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt
    25981 ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat
    26041 tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc
    26101 gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat
    26161 tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac
    26221 ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat
    26281 gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat
    26341 agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa
    26401 aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga
    26461 agataagatc attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa
    26521 tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga
    26581 agatcaatct cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca
    26641 ggtaatcgaa cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga
    26701 ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg
    26761 tgacaaaaat cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct
    26821 tcttcggaaa taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct
    26881 tacgcttacg attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc
    26941 aattcttctc ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga
    27001 atcccaactt cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt
    27061 gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt
    27121 actggtttgc gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta
    27181 tcgttgacca catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca
    27241 tctaattttt cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg
    27301 tcttcagtct tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt
    27361 cctggtgtca tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc
    27421 attggatctg ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca
    27481 gtatcttgtg tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg
    27541 atgacaaatt ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt
    27601 gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa
    27661 gaacggtctt cttcaccatc catccgattt tcatctgttt caacatctat accagacaat
    27721 ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt
    27781 tcataccctt cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag
    27841 cttccgatga ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa atagatttat
    27901 gattggtata ttgtt
//
LOCUS       DBKOIJ010000019        27674 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000019 DBKOIJ010000000
VERSION     DBKOIJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.019"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(258..2150)
                     /locus_tag="ACV5NJ_000648"
     CDS             complement(258..2150)
                     /locus_tag="ACV5NJ_000648"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484764.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2147..3874)
                     /locus_tag="ACV5NJ_000649"
     CDS             complement(2147..3874)
                     /locus_tag="ACV5NJ_000649"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484765.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3890..4540)
                     /locus_tag="ACV5NJ_000650"
     CDS             complement(3890..4540)
                     /locus_tag="ACV5NJ_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6484766.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4744..5040
                     /locus_tag="ACV5NJ_000651"
     CDS             4744..5040
                     /locus_tag="ACV5NJ_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HHY6484767.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5212..5832
                     /locus_tag="ACV5NJ_000652"
     CDS             5212..5832
                     /locus_tag="ACV5NJ_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HHY6484768.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5924..7090)
                     /locus_tag="ACV5NJ_000653"
     CDS             complement(5924..7090)
                     /locus_tag="ACV5NJ_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHY6484769.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7102..7455)
                     /locus_tag="ACV5NJ_000654"
     CDS             complement(7102..7455)
                     /locus_tag="ACV5NJ_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HHY6484770.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7583..8605
                     /locus_tag="ACV5NJ_000655"
     CDS             7583..8605
                     /locus_tag="ACV5NJ_000655"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HHY6484771.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8624..8785
                     /locus_tag="ACV5NJ_000656"
     CDS             8624..8785
                     /locus_tag="ACV5NJ_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484772.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9044..9478)
                     /locus_tag="ACV5NJ_000657"
     CDS             complement(9044..9478)
                     /locus_tag="ACV5NJ_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484773.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9588..11447)
                     /locus_tag="ACV5NJ_000658"
     CDS             complement(9588..11447)
                     /locus_tag="ACV5NJ_000658"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HHY6484774.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11448..11588
                     /locus_tag="ACV5NJ_000659"
     CDS             11448..11588
                     /locus_tag="ACV5NJ_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HHY6484775.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11565..12725)
                     /locus_tag="ACV5NJ_000660"
     CDS             complement(11565..12725)
                     /locus_tag="ACV5NJ_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HHY6484776.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13032..14456)
                     /gene="araA"
                     /locus_tag="ACV5NJ_000661"
     CDS             complement(13032..14456)
                     /gene="araA"
                     /locus_tag="ACV5NJ_000661"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HHY6484777.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14472..15170)
                     /locus_tag="ACV5NJ_000662"
     CDS             complement(14472..15170)
                     /locus_tag="ACV5NJ_000662"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HHY6484778.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15127..16788)
                     /locus_tag="ACV5NJ_000663"
     CDS             complement(15127..16788)
                     /locus_tag="ACV5NJ_000663"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HHY6484779.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17006..18406
                     /locus_tag="ACV5NJ_000664"
     CDS             17006..18406
                     /locus_tag="ACV5NJ_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HHY6484780.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18658..19737)
                     /locus_tag="ACV5NJ_000665"
     CDS             complement(18658..19737)
                     /locus_tag="ACV5NJ_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6484781.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19823..20782)
                     /locus_tag="ACV5NJ_000666"
     CDS             complement(19823..20782)
                     /locus_tag="ACV5NJ_000666"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HHY6484782.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20794..22305)
                     /locus_tag="ACV5NJ_000667"
     CDS             complement(20794..22305)
                     /locus_tag="ACV5NJ_000667"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HHY6484783.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22327..22890)
                     /locus_tag="ACV5NJ_000668"
     CDS             complement(22327..22890)
                     /locus_tag="ACV5NJ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HHY6484784.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22896..23759)
                     /locus_tag="ACV5NJ_000669"
     CDS             complement(22896..23759)
                     /locus_tag="ACV5NJ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6484785.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23773..24669)
                     /locus_tag="ACV5NJ_000670"
     CDS             complement(23773..24669)
                     /locus_tag="ACV5NJ_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6484786.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24844..26133)
                     /locus_tag="ACV5NJ_000671"
     CDS             complement(24844..26133)
                     /locus_tag="ACV5NJ_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6484787.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26105..27622)
                     /locus_tag="ACV5NJ_000672"
     CDS             complement(26105..27622)
                     /locus_tag="ACV5NJ_000672"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HHY6484788.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8071 a         5844 c         4790 g         8969 t
ORIGIN      
        1 tagctcctcc tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
       61 cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga tacaaaacgg
      121 aaaaatagct tctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
      181 caccgtttat tcggaaaaag ggactgtgac acgacttttg tcacagcccc taaaaaaaat
      241 aataaatttg tactattcta tgaagcttct tcttcagaaa actgactgtt ataaagatct
      301 gcatagaatc cgtctttttc catcaatgta tcgtggtttc ctgtttctac gatcgaacca
      361 tgattcatca caataatgtt gtctgcatcg cgtattgtag acaagcggtg agcaacaacg
      421 aaacttgtcc ggttttccaa taggcggttc atcgcttttt gaatcagtat ttctgttcgt
      481 gtatctacac tagatgttgc ttcatcaagg atcaggacat ctggatttgc taagaatgcc
      541 cgcgcaattg tgattagctg acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat
      601 acagtgttat atccttctgg caatttacga acaaaatcgt ctacgtgagc agctttagca
      661 gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat atttgatatt gtcatagata
      721 cttcctgtaa acagccaagt atcttgcagt accattgaaa actgtgcgcg caattcatca
      781 cgtgaaagat cacgggtatc tacaccatcg taacgaatac tacctccaga aacatcgtag
      841 aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg
      901 accatctcac caggtttgac atccaagtta aaatcagtca ttagcaagtt atctggtgtg
      961 tagccgaatt gaacattttc aaaacgaact ttgtatggtg tatctttttc ttctggaagg
     1021 ccagattttt cattggacat ctcttcttca tccaacactt caaatacccg ttcggctgaa
     1081 gcaactgttg attggatagt gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac
     1141 tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg tcatgttccc atttgcaact
     1201 tttactccgc ctagtacagc gacgaacaca tagcctagat ttttaacgaa attcatcaac
     1261 ggcatgatga ttgccgaaat aaattgagct ttccaaccag cggcatacag attctgattt
     1321 tctttttcaa atacttcctg gtctttttca gtatgattga atgttttaac aatggtatga
     1381 ccaccatatg tttcttctac ttggttattc aataaaccta aacttttttg ctgatctgcg
     1441 aaaaacactt gtgatttcgg cgctacgatc atcacgatga tcaagcttaa agggatcatc
     1501 aataacgcga ctaacgtcaa ttgccagctg attgaaagca tcatcacgat gaccccaatc
     1561 aatgtgactg cactagttac aaattgagtc aaattttgct gcagcgtgct tgcgatgtta
     1621 tccatatcat tgattgctcg agacatgata tccccatttg tatgtgtatc ataataggaa
     1681 atcggtacac gattcatttt ggcttccaaa tcatgacgta attcataaac tgttcgttgg
     1741 gaaacacgtg tcataataaa ttgctgtaaa aagttgaata cagcagaaat caaatacatc
     1801 acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact
     1861 tgttggcctt gttgcatctg ttgagcgcca gccattacac ctttgaatat ttctgtggtt
     1921 gctttcccca agacctttgg tgtttggatt tggaagatca cggctgctat tgctagtaca
     1981 aatacagcga tgatcgcaac catacgttta gacatatagc caaacaatcg gcgtaccgtt
     2041 ccccagaaat ttttaggctt ttcacctttt acaccgagat ttctgccagg tccaccatgt
     2101 cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg ttttactcat gctaagtcct
     2161 cctctcttag ttgtgaatga acgatttctt gataagtttc gtttgttgct aatagttctt
     2221 catgtgttcc tttgccaacc agttttcctt catctagcac aaggatcata tctgcatcca
     2281 ttactgtact tactcgttga gcaactaaaa cggtgatact ttctttcata ttcgttttca
     2341 atgcttggcg aagattggca tccgttttga aatccaacgc tgagaaggaa tcatcaaaca
     2401 catagatatc tgctgttttg actaatgccc gtgcaattgc cagacgctgt ctttgtccac
     2461 cagagaagtt tcctcctcct tgttccacat ggctatctaa gccatcttcc atctctgaaa
     2521 caaaatcttt agcttgtgcg atttctaatg ctttccaaat catttcatct gttgcatccg
     2581 gcttaccata ctgcatattg tctcggatcg ttccagagaa cagaactgct ttttgaggaa
     2641 caaagcccat taattctcgc aaattatatt gagtcatgtc actaatgtcc gttccattta
     2701 tttggatcga accagattcg atgtcatata atcgagggat caaattgact aaggtcgttt
     2761 tacctgaacc agtcccccct atgattgcga cgatctcgcc cgacttggct tggaaatcaa
     2821 tatcttccaa tgcaagtttt tctgctccat gatatcgata attgacatga ttgaaggcca
     2881 atgttgcgtt ttttccagca aaagaaactg ttttaggatt ttcagggtca tggataccga
     2941 ttttttcatc caacacttca ttaatacggt cagctgaagc ttgtgcacga ggcaccatga
     3001 taaaaatagc ggaaagcatc ataaagctga tcaagatctg catcgcataa gtcataaatg
     3061 caatcagatt accgacttcc aaagtcatat ctgctatgta gtgtccacca aaccaagtga
     3121 ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg catcatcaat gcgacgatcg
     3181 tattaacttt gattgccgta tttgtataat ctttattcgc taaatcaaaa cgattttctt
     3241 caaagcgtgt cttatcgaat gctcgaatca ctctgacccc tgtcaatcct tctcgaaata
     3301 ccaggtttaa acggtcagtt ttcttttgca tacttttgaa taatggaaca gcaaagtaca
     3361 tgattccgcc gatcaaaata atcattactg ggatgacaac taagaagatt tttgtcaact
     3421 gatgatcttt ttgataagca agtatgctcg cgccaatcaa cgtgattggt gcattgatca
     3481 tcatacgtaa aaacatttgt gtaaccattt ggatttgagt cacatcattg gtcgttcttg
     3541 tgatcaaaga ggccgttcca tatttatcaa acgcatcttt tgtcagactc tctgattttt
     3601 tatagacatc cgtacgcaat tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg
     3661 tatttgcgat agcagctaag atactgatca aagaaaagcc gatcatcaca aaacctgtat
     3721 gccaaatata atcgacatct ccttgcgcta cccctttatc aataatattg gaggtaagtg
     3781 tcggcaaata taaatctgcc agtacttgga tcaccatgaa aactgcagct gcaattgctg
     3841 aagtcaagga gatccgtttt acgattttta gcatctagtt cccttctttc tatttatata
     3901 ctccgttttt cagccaagaa agtttcaaat tgaattcact gattgcttgc tctactgagt
     3961 aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat acagtattca
     4021 tcactaattg gatcagtaat tccaattcat gatcattttt tactttcagc ttcgtccgat
     4081 cgatcaattc aagcaactct tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg
     4141 ttttttggcg actcgtatga ggagcgactt ttcttgaggc acggtaatcc atgttcataa
     4201 acaagttacg ataaaaagaa gcattctcgc cttccaaaat ctcataaatc attttagaaa
     4261 aataggattc gaatcctttg aatagatcac catcggattc tttcattagt tgtaacatat
     4321 ctttagagct gtcacgtctc attgtctcaa agtaataaaa ataaagatcc tctttatctt
     4381 caaaatactg atagaaactg cctctaggga tatccgctaa tttgacgata ttagcaatcg
     4441 aagcttcttt taagggtact ctagaaaact cgattctcgc agcctcgatt agtctttttt
     4501 gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc ctttctttca
     4561 agatgacaca ttgtcatatg acgctgtgtc attataacac ctcctacctg ttttgcaaga
     4621 catttgcata gtttttttca ttatcatccg atacattaaa aaagtgataa gaggaatcct
     4681 tccatttgaa ttaatgagag ttgcccatta aaatagccac atactaaaca aaggatgaga
     4741 taaatggccc aatcaattag tgtccaagac ttcaataaat tgcctctcga tgctgacagc
     4801 atcgttttag atacaagaaa taccttagat tacacaaaaa accacttaag ccactcgctt
     4861 tctatccccg ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa gaaactttat
     4921 tatattctaa gctatacagg caggcgatca gaagcattag ccgatcaact tactgctaat
     4981 ggttttcgtg ctgtttatat cattggtggg atggaagctc ttcagtcgag tgttgcttga
     5041 atattgcttg ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag cctcttttat
     5101 accctgtctt tttttagtta tttattcttc aaccttatca atatgacttg atagcctttt
     5161 ctttttctca tagttataac aatcatagca ataaaaggag aaggtaagca tatggcatct
     5221 gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt taaggcattg
     5281 acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac tgttttggaa
     5341 ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg ggaagcatta
     5401 cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc
     5461 aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg
     5521 aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt agctggaaaa
     5581 acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa agcactgcac
     5641 atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata tgtaaaaggt
     5701 attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg aatcgattat
     5761 gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc tgctttaggt
     5821 aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa
     5881 gcagcatttg taccttggat attttcccat ctgtctcttt ttgttattct ttctgctttt
     5941 tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa acatgagtaa
     6001 tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg attccgaaca
     6061 atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct aactgattcc
     6121 ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca aaaacgatca
     6181 gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc ggaatcatcg
     6241 ctaaaaatga tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga
     6301 cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc acagcaaaag
     6361 ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca ctcagcacat
     6421 tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt gctaattgtt
     6481 taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc actaaaatag
     6541 tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat atatcttgga
     6601 tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt tcgcgaaatt
     6661 ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta tctacatatt
     6721 gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg atcactacgc
     6781 tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg taaagccttg
     6841 gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac aagattcctg
     6901 ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca aacaaatatg
     6961 aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt aaggctgtga
     7021 ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc ttcttatttt
     7081 ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac caatagcagg
     7141 cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg gacaagcatc
     7201 aaaaatcaat gatacgtgag ctactgtctc taaattccca ttggccatcg gaccgacata
     7261 atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca aagaaaaagg
     7321 ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg ttgctgtttt
     7381 atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa tttctgtaat
     7441 ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta taacacattc
     7501 tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac tatttcatta
     7561 caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct atacgcgtcg
     7621 cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat tagccaacct
     7681 ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag ctggcaatct
     7741 ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata aggattttca
     7801 ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc
     7861 acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct accatatatt
     7921 aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga caggtcctca
     7981 taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc aacgattagt
     8041 cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt
     8101 gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc ttgtctttca
     8161 tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc gcgtgctttc
     8221 ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct tgccagaaga
     8281 attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca agttccttta
     8341 tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag atggttccca
     8401 cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt tcgcactaga
     8461 tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga cattatatcg
     8521 ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg taccagaatg
     8581 tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc agcaagacgt
     8641 attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa tcgaagataa
     8701 agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact actttgaaca
     8761 atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag aacatcagga
     8821 caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt
     8881 agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt gttgttggtt
     8941 ataggtatgt cattatcata aaggattttg atcactctat cataaaggta atattacatt
     9001 tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt ttcaacaaca
     9061 aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct tgttttgctt
     9121 cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt tggatcattt
     9181 cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga atgcccattg
     9241 cttttgctgg tggacgcaaa gtactgactt tcttaagcat tgcccgatat ttttttgttc
     9301 cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca cttgtttttc
     9361 cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct ccgattgctt
     9421 ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc ggtaacatgt
     9481 tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt atcctcaaat
     9541 gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta ttcgtgctca
     9601 aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg gatcaattga
     9661 tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata ccactgtccg
     9721 ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga taaaagcagc
     9781 tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc aaaataaaac
     9841 ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact gtcgaacata
     9901 agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt atctgaatag
     9961 atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag attcttcaat
    10021 tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag ataattctct
    10081 aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt atcatacttt
    10141 ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg tgttttttct
    10201 tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata tggattattc
    10261 tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa tcctaatgtc
    10321 tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg
    10381 tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt tggtacaagc
    10441 atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc agtcaatgct
    10501 tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt gccactatat
    10561 gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc tacgccgttt
    10621 aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg cgactcttcc
    10681 tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat ttctttgatc
    10741 ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt gtacaaaaac
    10801 ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat ccatagtgcc
    10861 gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact gatataccct
    10921 agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg ccttcctttt
    10981 ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc ggcagctaag
    11041 atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc agtgatcatc
    11101 ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata atttgcaaaa
    11161 cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga tcctgctagt
    11221 gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa tttttccgta
    11281 tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg acaccactgc
    11341 aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc tgttcctttt
    11401 aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg cttgctcctt
    11461 catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa ttcgaaagta
    11521 aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg cctttctggt
    11581 ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg tttcacttga
    11641 ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg ctcattctta
    11701 tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga aaatatctct
    11761 gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac
    11821 attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag ataatctttt
    11881 aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga tccccctaga
    11941 tgaggcaaca taagtatacg gtcattttct aagaactctt cttctggaaa atcagtcaag
    12001 tatttagcca gatgttgttt ttccaatgct tgcataacag catatttatc aacaatttca
    12061 cttcttccta cattgataag tacagcatct tttttcattt tttctatatt cgcctgatcg
    12121 atcaatcctt ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa
    12181 agaactgttt ctaaatctgc ttgccgaaca taatccaact gggcatcgcg gatagaataa
    12241 cccaagacat ccatccctaa gctatagcaa cttagcgcta cttttgtgcc tatcgctcca
    12301 agaccaagaa gaccgatggt tttgccttgc aattctcgtc ccacataagc acttcttttg
    12361 ttttctgcct gttctaaaat attaggacct gttagtgttt gtaccatacg acttgcttcg
    12421 attatcggac gggaagataa gagcaaacag cacaaaacaa gctcttttac tgcattagca
    12481 tttacacctg gtgtattcat gacgatcgtc ccgttttcag aagctttttc aacgttgatc
    12541 gtgttcaccc caacaccagc tcttgaaatt gctagcaaat ctggtgtaag ccattcatct
    12601 ttcactttcg tactacgaat aactattcct tcggctggca aatcatctat tattttgaaa
    12661 tattcccctt gtaacttttc tatctcttgt tctggaatag catcaatcaa atgaacgtga
    12721 tacataaaac cccctcctta ctaacaagta tctcgaaatt tctgcaatta gacaactgtt
    12781 taccatttga cttcctagaa gcaaaaagag cagttagagc agagtaggct gagtgataaa
    12841 aataaggcta tgacaagtta ctgccataag ccttattttc gagagcaaaa tattgagggt
    12901 gagatatctt gataaatggt gttccgttag ctgacattga taataagact aaatttcaaa
    12961 aaagttgaaa agcaaatttc gtcaatctct tcttatttct taaaactaag tggctttttc
    13021 acaacctctt tttatctaat aaccactgtt tccaactcaa ctaattttgc ccacgtttca
    13081 atttgatctg ttgttagatt tagcgagaca acggtatgat gaccgccacc tgcctgaatc
    13141 catgaatgga caccttcatg gaaattcggt ttagttttcc ataacacacg tgcaacaggt
    13201 aaattaggtg cttcagtcgt tggttcaaag gcttctactt cattgatcag cagtttataa
    13261 tgcgtaccga aatctgccat cgatacgact accccttctc ctgctttccc atcaaataca
    13321 agacgcgccg gatcttctct atctcccatt gataaaggag aaacaacgat tcttggtttc
    13381 gtcgcagcaa gcgtcggatc tacttccatc atatgggatt ccaagattgc ttcttggcca
    13441 ctagctagct cgtaagtata atcttccatg aatcctgtat ctttccctcg agccatgatt
    13501 ttcagcaacc gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa accgtatcct
    13561 tctgccatca aacgttgtac agctagtcca ggtagttgtt tcattccata caggtcttca
    13621 aaattagtcg taaaagcgtt atagcctcct tcttctaaga aacggcgaat accgatttcc
    13681 tgctgagctt gtacttttac atgttcctcc catgtctttt cttcataatc accatagtcg
    13741 aaggtataca attccttgta ctcttcaaat aattgatcga cttcttcgtc tgatacgcga
    13801 tcgattactt gtacaagatc tccgatacca aaataatcaa cagtccaacc aaattgaatc
    13861 tgtgcttcca ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc accaaaacga
    13921 gccactttta ttccaaaact ttcattatag gctactgcaa catccatcca atcagcaatc
    13981 tctttttgga tctcaggtcg tttccagtag ccaacgacga ttttattttg tttatttaat
    14041 cgagcattga tgaacccata ttcgcggtca ccatgagcgg attgatttaa attcatgaaa
    14101 tccatatcga tcgttttcca aggaatgctt tcgttatatt gcgtagctaa atgaagcaat
    14161 ggtttttgta gaagctttgt cccgcggatc cacatttttg ctggtgagaa agtatgcatc
    14221 caagtgatca cacctgctac ttcttctcga taattgacct ctttcatgat ttttgtgatc
    14281 gtatcagcag taactgctaa ttcttgaagg cggattggat aaggcaattg gccattttca
    14341 ttcagttcat cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca
    14401 tataaatgct gtgatcctac aacaaaccaa aattcttttt ctccaatatt caacataatt
    14461 agccactcct tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa
    14521 tgtttatcta aaatccgttg aggcagctct tctgaaaatg aattgagctg tctggtaaat
    14581 aaattcatct tgcatacttc atctagtacg actgcgttca ttaccgcact ttgcgcatct
    14641 ttcccccaag taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc taagggatca
    14701 attttgcgtt ctttgaatgt ttctatgata acttttcctg tttcatattc gtacccgctg
    14761 tcgatttcct gttgtgtcaa aaatcgagca caaggtaccg atccataaaa agtatctgca
    14821 tgcgtcgtcc ccatcgcagg aacatccaat cctgcttgtg cccagatcgt cgcccatgtc
    14881 gagtgagtat ggacgattcc tccgatttga ggaaatgaac gatataaaac agcatgagta
    14941 ggcgtatccg aagagggatt gagtttacct tcaatgatat gattgtccaa gtccacaacg
    15001 accatgtcgt ctgctgtcat ctcttcataa ctgactccgc taggtttgat aacgaaatat
    15061 cgtgtttcag gatcaaaagc gctcacattt ccccatgtat atttgacgag tccgtgttta
    15121 ggcaagtcaa gatttgcttg gtatacttct tctttcagtt gttccagcat tttctatcac
    15181 tcctttttct tgcaattgat gaatcgctgt tttttcaatg tcgatcccct cacggtaacg
    15241 tctcataaat atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg
    15301 gtcttgtcca aacacgtctt ctgccaagaa gttttctaaa gacttcccat gtcgatccat
    15361 catataggct gctaataacg cgattcccca cgctccacct tctccggctg tgtccatcac
    15421 tgtaactggt gcctccatag cagatgctag aatccgctgt ccaacttctg gtgttttaaa
    15481 aatcccccca tgccccacta aacgatcaat ttggacattc tcagatttca atatatccat
    15541 tccgattctc attgcaccaa aagcactggc taaatggatc cgcatgaaat tcgctaaatc
    15601 aaaacggcta cctggtgtac gtacaaataa tggccttcct tcatttactc ctgtaatgtt
    15661 ttcaccggaa aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc
    15721 tttgttgaat aataccgtga ataatttatc tctgcttatc tccataccca aagaattggt
    15781 aaattcttca aaaatactga cccaagcatt gatatccgaa gagcagttat tcgtatgaac
    15841 catgcctact agttctcctg aaggtgtagt gaccatgtcg atttccggat ggacattttc
    15901 tagttctttt tctaatacga tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt
    15961 ccgtttggct acactgttcg tagcgaccat accggttcct gcatcccctt ctggcggaca
    16021 aaccgggatt cctggtctca aatttcccgt agggtcgatc agataagccc ctgattcgct
    16081 taacttgcca gcttcttctc ctgctactag aactttaggc aaaatgtgat ccaagtcaat
    16141 cgctacgtgc gctttttcga ttaactgacc aaaaatattt ttcatttctt gatggtaggt
    16201 tttcgttgaa ggatcgatag gaaacatgcc tgatgcatct cctacgccta gtactttctc
    16261 accagtaagc tgccaatgga cataacctgc aagcgttgtc aaaaaattaa tttctttcac
    16321 atgttcttct ttattcaaaa tagattgata caaatgagcg atactccatc tctgcggaat
    16381 attgtattga aatttttccg ttaatattcg ttctgcttct tcagtgattg tatttcgcca
    16441 agtacgaaat ggtactagta actgttcctt gctatcaaat gctagataac cgtgcatcat
    16501 tgcactgaac ccaatcgacc cgattgtcgt aagattctcg ccatagattt cgaatacttc
    16561 tgctgccatt tttcggtaac tgacgcgcag tcctttccaa atatcttcta atgaataggt
    16621 ccagatacca ttttctagtt tgttttccca ctcataacta ccagcggcaa taggagtaaa
    16681 agatgaatca atcaatacag ctttgatcct agttgatcca aactcaatcc ctaaagatgt
    16741 acgaccatct atgacatcta acatactttg cgcatttact tccttcataa tgatttaact
    16801 ccctccttct cgtcaaacct gatcaaaccg tctttttagt agcgcttaca atgattattg
    16861 tatacttttg tacgtacatg tcaagcggtt atttacaaga atacaataaa aaacacttgt
    16921 tttgtacgta cataagttat aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc
    16981 gaaattctag aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct catcgtttat
    17041 ttattttttt ggcgcatttg gcggcattct atttggatat gatattggtg ttatgaccgg
    17101 cgccttacct tttttacagc atgattgggg tttggcaggt aaagcttctt tgataggatg
    17161 gatcacgtct tcggtcatgt taggtgcgat ccttggcggc tctttatctg gtcttctttc
    17221 tgataagctt ggacgacgaa aaatgattct tctttctgca cttattttta tggctggttc
    17281 tgtcctttct gcatctgccc ctcacaatgg ttcttatttt ctgattgcag caaggattct
    17341 tctaggttta gctgttggcg ctgcatctgc cctagttcct gcttatatgt ctgaaatggc
    17401 tcctgctaga ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg cttctggcat
    17461 gctcctttcc tatgttgcag attatttgct aaaagtgttg cctgaaacga tggcttggcg
    17521 cgtgatgtta gggctcgctg ctgtccccgc gctgatttta tttttcggtg ttttagctct
    17581 accagagtca ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt
    17641 gaactatatt cggacaccaa aagaagctga acaggaattc gaacaaatcc agctgaatgt
    17701 caaacaagaa aagacaaccg gaacttcttg gcatactttg tttttagaaa aatatcgttc
    17761 cttagtcatt gccggtattg gtgtagcagt attccagcaa tttcaaggag ctaatgctat
    17821 tttttactat attccgctga tcgtagaaaa agcgactggg catgcagcga gcgatgcatt
    17881 gatgtggccg atcatccaag gaatcatttt agttgctggt tctttgctct ttttggttat
    17941 tgcagacaag ttcaatcggc gaactttatt gaaaataggc ggtagcgtga tgggactttc
    18001 attcatccta ccagctgttt taggaactgt actggatgct catacaaatt cgttgttgat
    18061 tctcttgttt ttatgtattt atgtcgcatt ttattcttgc acatgggctc cactgacatg
    18121 ggtaatcata ggcgaaatat tcccactagc tgtccgcggg cgagcatctg gcctagcctc
    18181 ttcttttaat tggattggtt ctttcctagt tggcttgctt tttcctgtga tgactgcttc
    18241 tatgtctcaa gaaatcgttt tcggcatttt tgggatcatc tgctttttag gggttttatt
    18301 tattcaagag atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc
    18361 atctaaaaaa acttatccta aacgtatttc tatccatcat tcttaaagag gttgtgacag
    18421 aagtggtcag cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca aggaacaatt
    18481 tcaaaaaaca gcttctcatg tttgatgaat attgtgactt gtgaccagtt ggtaggagaa
    18541 caccgtttag tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga caaaagctct
    18601 cacaaacatt gcgacgagta accgtaggag caataagagg cggtaaaaac ccgtctctta
    18661 tttttcgttt tctttcactg tttcccgttc aactagtaca ggttcataga gaatatttgg
    18721 ctgtgcctgt cctttttcaa tcgttgtgac gatccaccgt gcggcatctt gtcccaactt
    18781 ttcttttgga tgcgtcaatg ttgtcaattt tttattccct aaatggctta aagaagaatc
    18841 gtctgttcct acgatcgata ggtcatctgg aatatgatag ccattttctg tcaacttatc
    18901 tagcattaaa ctagctattt gatcgttata gcaaatgatg ccatctaaag cttgttcttc
    18961 tttcaaccgt tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat
    19021 gatatcttcc gtcaaaaaac gcacattata ctgttcacac gcgctgataa aacctttcat
    19081 cctgtatttt ccttgaagat cgtcgatttt tgtgatgaac aacagacgct gatgtccttt
    19141 ttttatcaaa tattctgctg ccataaaacc gactttcaca tcatctaaac aaatataaga
    19201 ggcatttaac tcttcataag cagcattgat catcaccatc ggaatgcctt gttctctcaa
    19261 agcaacataa agtgctaagt ttgggttata ctgattactt ttcgtaggct caacgatcaa
    19321 accatctacc ccataagcaa tcattttctt caaacactct ttctccaatt cgtgatcgtt
    19381 attcgtactg gctaaaagca atgaataccc agattctcgt aattcatttt cgattcctcg
    19441 gataatcgac ggaaaaatat aatcagagag atatgtggtg atgacaccga tcgtttttgt
    19501 tctgttcaat ggtgtttttt ctttcttata gctgtcatct acatatgtgc ccgacccttt
    19561 ttctttacgc agatagccct catttaccaa cacagcgatt gcttgccgaa tcgtatgtct
    19621 gctgacgtca tatatttctt gcagcttgaa ttctgaggga atggttgtat ttacttcgta
    19681 ttcctcacga aggatctttt gttttatctc ttctgcaata tcttgatatt ttgccatttg
    19741 ccttgcccct ttttatttgt acgtacatat atcataacga gtctgcaagg tttattcaag
    19801 caaaaatcaa ggtttcttgt gctcataagg catacggaac aggttcacca aattctggaa
    19861 atccttgctc attccacgta aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg
    19921 gatccccttt ttggttcttc tctggtcgag catgatagat caaaatatca aagcacccgt
    19981 cttctgaaac cgtaaacgag ttgtgaccag gaccaaattg cttatttttt tcagaagaaa
    20041 cgaatactgg ctccgataat ttatgccatg attcttttaa taaaagattc ttgtgctcat
    20101 cagcccacag caggcccatg gcatagtttt catctgttgc actaccagaa tacgtaatga
    20161 atattttccc attgcgagta aggactgctg gaccttcatt tactgaaaaa ccccgttttt
    20221 cccattcata ctcaggtata gaaaggagga tctgcttccc ctttaacgtc caaggattat
    20281 ccatttcgga tagataaaga ttagaattcc cagtaatttc aggatcttgt tgggcccaca
    20341 cataatatag ctttccttga gaagagaaag ttgttccgtc taaactgaaa gtttcccttt
    20401 tcgtttttat ttgtcccttt tctatccaat ctgtattcat tggatctata tgctgatttt
    20461 caagtacgaa catgcggtga tgatgagatt catcacgtat agttttatga ttgcttgcag
    20521 caaagtaaat ataccatttt ccattgatat aatggacttc aggtgcccag atcaattcac
    20581 tcatcggtcc ttcttcatga gcctgccata caatcaggct tgcagcatgt tctaattcat
    20641 tcagtgactt tgcccttctt aattcaatca cttggtagcc tggaaccgat cctgtaaaat
    20701 aataataacc atctgtatgt ttatataccc aaggatctgc ccgttgccga acgataggat
    20761 tattataaag tgtcttcatc ataacaaatt tcctcacatt ttctttattc taattacatt
    20821 ccaagagtat ttttctatcc ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt
    20881 tatttcaggt tttatttttt ctggttcttc acgtgtattt ttgtccaaca gatcgctact
    20941 ggttaatgtg tagtgctctc cttgatctag caaattgaaa tcatgttcaa tcgttatctt
    21001 ttgttgttca tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt tatctacgat
    21061 cacactgtcc acataaggaa cattttttat cttttcacaa tcataaacag ggcaaatgat
    21121 ttgttgtttc aatacttgac ctttagcata tttcgataag tgttgcatca cataataagt
    21181 tggattcctc catgcttgtc ctttagcatc tgtcagtatt aaaggtattg tgtttactaa
    21241 caatgattga caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga
    21301 accaaataat aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac taccgacttc
    21361 aaaatcccta tgtctttttt ccggttgggc atgtacattc cattcatcaa acgctagata
    21421 cattgttttc tcacttctca aatatccttt tacataatca caagtagcaa cgatttcttc
    21481 aatttgacga tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat cacgctcatt
    21541 tttaggaggt ttagtcaagt cttcttcatc atacttatca atatagttat gcaaagctaa
    21601 ataatctaca ttctcataag cttcttctaa tgcgatacga tcccattcag ggtacgatgg
    21661 taaacgtggc gtagagcttc caactaagat tgtttcaatt gaatcatcca ctaacttcat
    21721 gacttttgag gcttcatttg ctaacctacc atattcataa gcactcttag cccccacttg
    21781 ccatggaccg tctagctcat tccctaaaca ccataattta acgccaaaag gctttttttg
    21841 cccatttttt attcttaaat cactccagaa ggttcctttt ggaaaattac aatattctac
    21901 taaatttttt gcttcatcaa ttccttttgt tcctaaattc accgtgaata tcggtttcgc
    21961 ttgtacatga ctgatccatt taaaaaattc gtggagtcca aaagaatttg gttctatacc
    22021 tcgccatgct aaatcaattt tttttggtcg ttgttctttc ctacctacag tatccaccca
    22081 atcataacca gatgtaaagt ttcctccagg atagcgaatg acacctaatt ctaattcctt
    22141 tacataagag aggacatctt gtctaaaccc gtcaccatca gctgtttcat ggcccggttc
    22201 ataaatccct gtatagacga cacttcccat atgctcaaca aatgagccaa acagccgaga
    22261 actgatctct cctaccgtaa aatcctctct gatttttact ttcacttctt cgtccaacag
    22321 ctttccctac ctttcatttc ctatagtcaa gataatagat acaagtttcc atacgccacc
    22381 agcaaagata aaaataaaag gagggaaaag aaatatgagc agtacaaatc ctaataacaa
    22441 tacgcaacaa agaatatttc tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa
    22501 aatttgtaaa aatgtgatat ctttgttttc agaaatcaca tatatccagg ataaaagaaa
    22561 catggaataa ataagtaaaa ctacatatcc actaattgaa agtacgattg tctgttcttc
    22621 taataattga ttaacaagaa aataaataat gatcgttaac agactaagga acaagttctt
    22681 accaaaatgt tgctttattc cttgaaagaa aaaataaatg atatttcctg atccctcacc
    22741 tttccattgc gccattacat aaaaaacagc acatgtagca ggcacgatag taataacagg
    22801 caaacacacg attacccaga gaatacctaa taagatgtaa tcagaaacca caatcaactg
    22861 tccataaaat ttagtctcaa ctattttcat cttcctcact ctttcattcc agacatagct
    22921 gttccttgaa caaaatattt ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa
    22981 ataaccgttc cagccattac aggtccataa aaagaaatgt tctgccctgt aaataaagct
    23041 aaccctgctg gtaaagttct catttttgaa ctacttgtca atatcaatgg ataaagcaag
    23101 tcattccaac tattcatcag cgtgaaaatc cctaatgata aaagagtagg tttgctcaat
    23161 ggcaacatga ttttcaaaaa aatattaaac tcacttaatc catcaatgcg tgctgcctct
    23221 tccaaagaat ctggtaaagt aataaaaaag gaacgcatca tgaagatacc aaatgcagtt
    23281 gtcatacgag gaatgatcaa acctgcataa gtatcaagca tgccaagcaa attgacttca
    23341 ataaatagag gaatcatgaa gacttggaat gggatcatca ttgtcagcaa aacgaaatag
    23401 aacaaaactg attttccttt gaaacgcata cgagcaaatg cataccccgc aagagaatca
    23461 aagaatacag aagtgatcac tgtccctcca gcaaaaatca ctgtattttt gatgtaatct
    23521 aataaaggga tcgttgtcca aaccttaata tagttatcta gggtatagct atctgcaaat
    23581 attctaggcg gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat aatcagccac
    23641 aaaaaaggaa acgcaataac caacaatata actagcatac taatcgttcg aatgattttt
    23701 ttaccactaa ctgcttttcg actattcaca gtaactgtaa tcgagctttc tggtgccata
    23761 caggtttccc tcctatttca tttgtttttc tttccgtgac atgtacacat tagtggagat
    23821 agctatagtt gcaacaatga taaacaaaac cgaagacagt gctgatgcat agcccaattc
    23881 ataaggtgct gtaaatcctc gactgtaaat atattgaaca gctgtttctg tcttaaattg
    23941 tgggccacca gcggttgcaa catatacttg gtcaaacact tgcatcgaac caatcagatt
    24001 cgttaaaata caaaaaccta aggagggaac gatctgaggt agcgtaatgt taaaaaactg
    24061 ctgtcgattt gttgcaccat ccatttgagc agattcatac aaagagggag agattccctg
    24121 aatagcagtc aagagaatcg tcatagtaac accaaaattc ttccaaacag tcatgatggc
    24181 taccgtcggc atagcaaggg ttggatcgcg aaaaaattga gggatcggca agccgacttg
    24241 tgaaaacaag taaggaatcg cccctacatt cggatcaagc aacatagaaa aaataatcgc
    24301 aattgacgtc aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa aacgattgaa
    24361 tctggtattt ttagataatg ctactgctgc taataaacca acgataattt gtgttggcgt
    24421 ttcgagtacg gtgaaatata aagaatgaat caacgaatta actgctcgtt catcactaaa
    24481 tatacgtgtg aaattttcaa aacttacaaa atttcttgat gtaaagaaaa tattcaaatc
    24541 tgtaaaacta atagcaaatg ttcctaaaag cggtatgaca gtaaataata ataaaactaa
    24601 aaaagatgga gttaaaaaca aatacgcagc acgatcagga cgattccaat atttggtgaa
    24661 tccgttcata tagtccacct catctttcat tttgataaaa tatacaatct tggtttaaac
    24721 gaatctgaat aagatatccg aacaatcgct gtgatttttg caataacacc tatctgcaaa
    24781 tactaggtcg atcattcaga tatctgtatc ttgccaacag tctatcctgt ataaagttct
    24841 gtactatttt tgcagtaatt gattgatggc atcatctgca tcgttcatga gtgtctcagg
    24901 atcttctcct gctaataatt tttcaatcag tggattgatg atatcgttat tgatcgcaga
    24961 gatttgggta aaacctggta aatatggctc tgcatagtcc atttgtttcg ataattcaga
    25021 tacaatagga tcatttttta cttcttcatc ttttgctact gaatccaagt aggcaggaaa
    25081 accattttcg atactccatt ttttaccaat ttcagttgta ttccaatatt tcaaaaactc
    25141 atagacagct tcttttttat tattatctgt acttgccgga atggcatatc ctacgccatc
    25201 taaaatagct gtttttttgt ctgaatcact cccttgtggt aaggtagtca cattataatc
    25261 aatctcgttt gctttcaatc cgttatttaa ccatggccca ttgatttcaa tggctagttg
    25321 accagcattc attaaattgt ccatttcggc gccagaaatc gattctggac cagtcttgtc
    25381 tacatggatc attttttgga tcgcttttaa cgttttaagg ttctcaggag acgcaaattc
    25441 agacttagta taatcatcgt tcactaattt accgccattt gctaacatcc agttatataa
    25501 cggtgcagtt ccatccttat tgaagacaaa acctgataca tttttactac tatcagttaa
    25561 ttttggtgcc atttctgtca attcttccca agtagtaggt ggttgttcag tatctaatcc
    25621 agcttgttta aacaaatcat tattccaata catatacatg ccttgtacct gcatggggac
    25681 aaaatactgt ttaccttcta cttcacctaa ttcaatagcc gtttctttaa aatcagattt
    25741 atctactcca tcatattccc agaaatcgtc taatggttgt accgctccgt tttctacata
    25801 ctgtgcaaaa tctggataat ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc
    25861 tgtaggaagt ttttcattca aattggccca agtcatgata tccatttcaa ttttgatgtt
    25921 tttatcattt gttttattaa attcattaac gatttcttgc aatatttcac catcagatgc
    25981 agtaaaccca ttccaaaaag tcaaagtcac ttctttatca gaatcagatt ctgctgaact
    26041 tccgccaccg caagctccta aaatacctgt tgcagctacc aacaaagagc caattaacca
    26101 ttttttacct ttcatttttt gttcctcctt catctgataa acgacatctg accacattcc
    26161 aagatagcgg tttcaataaa caattgattt tcccatcttc agcttttaca tttgttgaat
    26221 gacttggttt tacttgttca cttttaggaa gattagttgc ttttgtatca aatccactca
    26281 tttcagaaaa atcaatcact ccctctaatg ataattcaga caagtgaaca gttagattca
    26341 tttcttcttc tccacggtta actgcaaata tgacaagttc tttgtcattt attacggcta
    26401 tactttctaa atacggcact tcttcaaaat cttccgtcgc atagtttgct gaacgaattt
    26461 gtgggcttaa cacctctcct tgtccataca cagcagcttg cataaatgga taatagatcg
    26521 tctgtctcca agcttctccg tttgtttctg tcctgattgg tgcaataacg tttaccaatt
    26581 gagcaagaca agcaatcttt actctatctg catgtttcaa taaagtgatt aatagacagc
    26641 ctaatagtaa tgcatcttca aaattatagt tatcttctag taaaggagga gcaatctgcc
    26701 aaggcgctgc ttgctcatct tgttccgctg aatgatacca aatattccat tcatcaaagg
    26761 acaggttgat ctgcttctta ctatgtttct tcgccttgac tgtatcgcac atcgctgtca
    26821 cttcagaaat gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa agatcatttt
    26881 tttggttgcc ataatattga tgcagagaca aataatctat ttgatcgtac gcttgatcaa
    26941 gaactgttaa ctcccaatca ccaaatgtag gcatttgccg attcgaactt ccgcataaaa
    27001 ccacctcaat tgaatcatct acgagtttca tcgccttagc cgtttcctct gctaaccgtc
    27061 catattcttc cgctgtttta tgtccaatct gccaaggacc gtccatttca ttccctaaac
    27121 accatgtctt aattccaaaa gggctctctt gaccattcga acgccttaaa tcactccaat
    27181 aagttccttt aggaaaatta caatattcga ctaaattacg agctgcatct actccacgag
    27241 tacccagatt gacagccata ttgatttcgg cccccacatg ctgacaccag ttataaaatt
    27301 catgaatccc aacttcattc gtttcaattg tccgccaagc taaatccaac cttctaggtc
    27361 tatttgctat gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag
    27421 gatatctaat catgggaatg ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc
    27481 cacttttatt agctgatgga tgatctggct gataaatccc ttcatatact gctcgtccca
    27541 tatgttcaat aaatgaactg tacaaccgcg gatcaacttt gcctacacgc ttgacactac
    27601 taatcaacat tgtggcttcc ataactgacc tcctttaatt tatacatgaa taatacacct
    27661 cgttgaaagc gttg
//
LOCUS       DBKOIJ010000020        27170 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000020 DBKOIJ010000000
VERSION     DBKOIJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.020"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(45..1613)
                     /locus_tag="ACV5NJ_000673"
     CDS             complement(45..1613)
                     /locus_tag="ACV5NJ_000673"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HHY6484789.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1617..2027)
                     /locus_tag="ACV5NJ_000674"
     CDS             complement(1617..2027)
                     /locus_tag="ACV5NJ_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HHY6484790.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2024..2947)
                     /locus_tag="ACV5NJ_000675"
     CDS             complement(2024..2947)
                     /locus_tag="ACV5NJ_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HHY6484791.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2944..3822)
                     /locus_tag="ACV5NJ_000676"
     CDS             complement(2944..3822)
                     /locus_tag="ACV5NJ_000676"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HHY6484792.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3809..5146)
                     /locus_tag="ACV5NJ_000677"
     CDS             complement(3809..5146)
                     /locus_tag="ACV5NJ_000677"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HHY6484793.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5149..6252)
                     /locus_tag="ACV5NJ_000678"
     CDS             complement(5149..6252)
                     /locus_tag="ACV5NJ_000678"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88/105 protein"
                     /protein_id="HHY6484794.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6326..7834)
                     /locus_tag="ACV5NJ_000679"
     CDS             complement(6326..7834)
                     /locus_tag="ACV5NJ_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HHY6484795.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7800..8492)
                     /locus_tag="ACV5NJ_000680"
     CDS             complement(7800..8492)
                     /locus_tag="ACV5NJ_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HHY6484796.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8514..9488)
                     /locus_tag="ACV5NJ_000681"
     CDS             complement(8514..9488)
                     /locus_tag="ACV5NJ_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484797.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9788..10660
                     /locus_tag="ACV5NJ_000682"
     CDS             9788..10660
                     /locus_tag="ACV5NJ_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6484798.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11118..13316)
                     /locus_tag="ACV5NJ_000683"
     CDS             complement(11118..13316)
                     /locus_tag="ACV5NJ_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6484799.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13548..14540
                     /locus_tag="ACV5NJ_000684"
     CDS             13548..14540
                     /locus_tag="ACV5NJ_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6484800.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14600..16021
                     /locus_tag="ACV5NJ_000685"
     CDS             14600..16021
                     /locus_tag="ACV5NJ_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HHY6484801.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16014..17513
                     /locus_tag="ACV5NJ_000686"
     CDS             16014..17513
                     /locus_tag="ACV5NJ_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HHY6484802.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17552..18946
                     /gene="uxaC"
                     /locus_tag="ACV5NJ_000687"
     CDS             17552..18946
                     /gene="uxaC"
                     /locus_tag="ACV5NJ_000687"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HHY6484803.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19105..19788
                     /locus_tag="ACV5NJ_000688"
     CDS             19105..19788
                     /locus_tag="ACV5NJ_000688"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HHY6484804.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19790..20626
                     /locus_tag="ACV5NJ_000689"
     CDS             19790..20626
                     /locus_tag="ACV5NJ_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HHY6484805.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20637..22391
                     /locus_tag="ACV5NJ_000690"
     CDS             20637..22391
                     /locus_tag="ACV5NJ_000690"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484806.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22667..23008)
                     /locus_tag="ACV5NJ_000691"
     CDS             complement(22667..23008)
                     /locus_tag="ACV5NJ_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HHY6484807.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23076..25247)
                     /locus_tag="ACV5NJ_000692"
     CDS             complement(23076..25247)
                     /locus_tag="ACV5NJ_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HHY6484808.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25458..26315
                     /locus_tag="ACV5NJ_000693"
     CDS             25458..26315
                     /locus_tag="ACV5NJ_000693"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HHY6484809.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26386..>27170)
                     /locus_tag="ACV5NJ_000694"
     CDS             complement(26386..>27170)
                     /locus_tag="ACV5NJ_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HHY6484810.1"
                     /translation="LSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDNY
                     GYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLTV
                     GTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIEA
                     RTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQQ
                     FVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         8065 a         5608 c         4885 g         8612 t
ORIGIN      
        1 ctagtgaaaa taagttagtg aacctcattt attaaagaat taaattacag atgatctttt
       61 ccatcatact tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc
      121 actgctatcc cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc
      181 ggacctactt tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct
      241 ttctttacag tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct
      301 atttgtgcta ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc
      361 caagtaacat aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc
      421 tgattaaaat tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt
      481 tgccgaatag ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga
      541 cgtaaaaaac ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg
      601 gcattccagt tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct
      661 tgttgtacct cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct
      721 ttaagtctta accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc
      781 aaaatagccg caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac
      841 ggggtttgta ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta
      901 gctgtcagca taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg
      961 caaactgtaa ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac
     1021 ccgttacgaa aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt
     1081 ttatagatgg gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg
     1141 ctggatttat tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt
     1201 cccgtttcaa gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt
     1261 tcggaccaag gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac
     1321 ggacggcctg cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg
     1381 atctgtggcg cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc
     1441 aaaatatccg ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct
     1501 ggaagccatt caaaagagga gacaataatg taataccgct ctttcactct taatatatta
     1561 ggatctggat tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag
     1621 gctccttttc tcattcttac tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat
     1681 gcaaaaggaa acgcaataaa ctgagtgcca atcaacaata tgaatcctgt tataaaatac
     1741 aaaccagtgg cagttgttaa gtattttcct ttagcagtca aaaacaatgc tactgctaaa
     1801 aaagcaagtg aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca
     1861 aatgtatttt ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta
     1921 aactctgacg cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat
     1981 agactgccac cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct
     2041 gcttttctaa atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt
     2101 cagagaattt gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt
     2161 gaacatgccc acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc
     2221 ttcctaaata gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat
     2281 accctgtttt ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg
     2341 ctaagctatg gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca
     2401 ccgttccttc aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag
     2461 ccggtgtaca aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg
     2521 tatcctgata accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata
     2581 aagcaacagc ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt
     2641 ctaatacgac attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc
     2701 taccgtcaag atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc
     2761 ccacaagttt aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta
     2821 ctatcaagac ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga
     2881 tagtttcaaa atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca
     2941 tcctcataaa tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat
     3001 atgaccgcct gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt
     3061 tttgtccagc ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt
     3121 tccaactaag ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata
     3181 agctgtcaat agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag
     3241 caaaagtatt tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc
     3301 caatgctgtc acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg
     3361 ttgagtatct gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat
     3421 acgcattccc cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga
     3481 aatacctccg cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc
     3541 tgtcagtact tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact
     3601 tttcccgact gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc
     3661 ttctttttct ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg
     3721 atagtattca agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt
     3781 atgtctgcta gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa
     3841 caaccagttc atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt
     3901 tagtattttc taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg
     3961 aaaccttacc cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca
     4021 tcatagcagg aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg
     4081 tgatttcttg tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat
     4141 tccttgccgt gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg
     4201 gataagcctt tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa
     4261 gattcagtat aaatggacac atcacattat ccatgactat attggatact cgaatatctt
     4321 caacgatccc gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga
     4381 agatacaatt tgaaatggta atattacgaa tactaccact catttcactt cctaatacga
     4441 caccgccatg tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc
     4501 tttcgtacgt atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat
     4561 ggcaattact aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg
     4621 aatccgctgg gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt
     4681 tgatcgtcca acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt
     4741 tatggaaact catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag
     4801 tatgccacca tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct
     4861 ctacattttg agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg
     4921 aatgaacaac aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta
     4981 aatgaagttc gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa
     5041 caactacggt cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag
     5101 tattcaattc atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc
     5161 cacttcaatc aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg
     5221 ttcactgatg tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac
     5281 ttgacagttt ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag
     5341 taatccttga tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa
     5401 acgtattcct ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt
     5461 tcctttccgt tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata
     5521 tggaagtaat gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc
     5581 ttcatgaagt aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct
     5641 ccaaaaatga tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc
     5701 ccatgcatga atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa
     5761 ctgtctcaaa acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata
     5821 aaaaggagaa cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg
     5881 ccaaaaagct ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc
     5941 tgctttttta tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt
     6001 atttacatgg tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg
     6061 cacgaaatag tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc
     6121 ccaaagtaat tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata
     6181 ccacttttct tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga
     6241 ccagttttcc attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa
     6301 gaagtatttg tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact
     6361 cttgtgtgat tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat
     6421 cttcgtcaag ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt
     6481 ctgatcctac ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa
     6541 attcttcatt ttctttgatc aattgattag agaattcttt ttcagggtct gtatctttga
     6601 aagagaacgt cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa
     6661 gatccatatc gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga
     6721 taccgccagt catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta
     6781 atttcttcaa ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa
     6841 taccatttcc ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta
     6901 caggcactaa ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat
     6961 ccaccattcc agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct
     7021 tttgggtaac tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat
     7081 tcaaagcttt catatactct tctgtatcaa attctggagt gaatttgcca ttatcgtcta
     7141 cagcccagcc atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa
     7201 tatcactagg cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat
     7261 cgttttctgt aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta
     7321 gtcctagatt atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt
     7381 gtatcggtac gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa
     7441 tttgacttaa atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt
     7501 ttaacgcatt gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc
     7561 ctagttcatc agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc
     7621 agttgaatgt cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag
     7681 gcggtgaagc atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct
     7741 ctgaagaact gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac
     7801 taagacctaa ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata
     7861 atatttttgt tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat
     7921 atagaatttg ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa
     7981 acacctggca ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact
     8041 aatgagtaat gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat
     8101 cctttccaat cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg
     8161 aacgcagcaa tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca
     8221 agtaaaacaa tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt
     8281 tctttaaaat atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat
     8341 tttctaatca ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct
     8401 gcgatgacta aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc
     8461 cttccgttta ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa
     8521 acgccgtctt ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct
     8581 acgacccctt taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca
     8641 gctgtataaa tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac
     8701 aagtacatat gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta
     8761 atgattactg gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct
     8821 ccatccattt ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag
     8881 atgatcgtag accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc
     8941 cattcagggg atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc
     9001 aatacccctc cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac
     9061 atgaagtgag ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact
     9121 tcatttaata gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg
     9181 aacaagatca acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg
     9241 aaatgtttga aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga
     9301 aaagcaataa cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc
     9361 ggcagaatca tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt
     9421 cttctgagct tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca
     9481 ctttccataa gcccctcctt agttttgtga atgtttgctg cttatgactt catgatagat
     9541 aactatctga attacgtata taatcatttt taccgtaagt aaaaacgctt acatagcaat
     9601 gatttttgtg ctatacaaaa cgataaagtg tattttttca caaataaact acgcatagca
     9661 aaccttctat ttttttctat ctgacactta gcataggaaa tgattatata cgtaatcttt
     9721 caccgatgat aaattgaaca tatagaaaca gaaacattta atttattttg aggaggagcc
     9781 tatctatatg aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac
     9841 gtttctaatt ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc
     9901 ttttgcttct acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc
     9961 tttagacgcg tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc
    10021 gattggtgtt acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta
    10081 cggtctttcc agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc
    10141 tatgttgttc agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt
    10201 caattcttac tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat
    10261 gagaaacttt ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg
    10321 cacagatttt ggtgtatttt tcaaaattat gctgccatta gcgttaccgt ctattgctac
    10381 tatctcgctt ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat
    10441 caacgattca tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag
    10501 tgggatgcaa gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat
    10561 ggctgttatc gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata
    10621 ttttgtcaaa ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat
    10681 ggtagttgtg tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt
    10741 cttaaatttt tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt
    10801 atcagaggtt gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt
    10861 gcgacgagta acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc
    10921 acaaaattat gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag
    10981 aactgtgaca agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat
    11041 ccataaatac aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag
    11101 aaaaacagcc tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc
    11161 ttctttttaa agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc
    11221 tcttttacgg agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt
    11281 tgaacaaaat caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta
    11341 gggttataat gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg
    11401 tagacgatat gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga
    11461 gagcattctg tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga
    11521 tgataattgt tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct
    11581 actcccaata actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc
    11641 cgactcaaag gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc
    11701 ttcacttcct gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga
    11761 tatctaataa gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa
    11821 cttggaccag tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg
    11881 ctttcattca gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat
    11941 tcttttacac gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca
    12001 tcatctttat ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc
    12061 attcgatttt cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa
    12121 tcacgctccc caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag
    12181 cagtcatttt gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct
    12241 actcgatttc taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact
    12301 aagtgtgcac ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa
    12361 tctagttctt tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa
    12421 atcggacggg caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt
    12481 gttaaagaca ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta
    12541 gagtcaactt ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt
    12601 ttatcttttt ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca
    12661 gttggttgct ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca
    12721 atagaaagta tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc
    12781 gtagcaattc ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg
    12841 attcctttgt ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga
    12901 tacttttcaa tttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta
    12961 ccagccaaac tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt
    13021 agttctttcc tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat
    13081 atgtccgaga aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt
    13141 ttccattcaa cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc
    13201 gattcgatcg attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg
    13261 gctcctaata aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc
    13321 ttcctctcct ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta
    13381 attaaatgag cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat
    13441 ttttaacgtg tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat
    13501 atgatgaatg cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca
    13561 aagatgtcgc taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca
    13621 gcagtttaat aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt
    13681 attcgccaaa ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat
    13741 ttttctcaag tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca
    13801 tccattcagt attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt
    13861 taacagaaat caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg
    13921 atcagccttt catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc
    13981 acatcaatga tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg
    14041 cagtagatta cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa
    14101 attttcgttc gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca
    14161 tccctgtcaa tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa
    14221 acatggaata cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg
    14281 acatagcgat tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca
    14341 tttctctgat cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca
    14401 ccgtacgtaa gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga
    14461 tcgaaggcag gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg
    14521 agtcgatcaa ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata
    14581 acacagaaag gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc
    14641 acctgtaaaa gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca
    14701 gattcagcag ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt
    14761 agaacaaggt cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca
    14821 aggactcagc aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat
    14881 gatcaatcct tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc
    14941 ctatatcgtc tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac
    15001 agatactccc ccacagagcc tcccgggcaa actgacagcc tttttatacc gacgtttcaa
    15061 attaaacaaa gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg
    15121 tctaaaagaa atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga
    15181 gtggttagaa aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta
    15241 tcctcgcgaa gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt
    15301 caaagcagaa ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact
    15361 accattcaaa caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga
    15421 acgtaaggtg catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc
    15481 ggaattggaa actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca
    15541 gttattcaat ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta
    15601 tgctgaccaa atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat
    15661 ttcattaaat agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat
    15721 cgaagaacaa aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat
    15781 gtatcgaggc gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga
    15841 tgcttggtct gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg
    15901 agaagatttg tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat
    15961 cgatcaaaaa ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg
    16021 aaaccctgca accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc
    16081 ctattccaaa acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata
    16141 tcccgcaagg tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat
    16201 atggctatcc aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc
    16261 ataatgtaaa aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc
    16321 caagtcatta cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag
    16381 tagggattcg taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg
    16441 aactgatcgt caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta
    16501 cgattttaaa gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag
    16561 aaattttagc ggacgcagta aatcatccca atgccggagg agttttagta tttggattag
    16621 gttgtgagaa caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac
    16681 gagtgaaatt tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat
    16741 tagaagagct tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt
    16801 tgaatgtcgg tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt
    16861 tacttggtgc attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag
    16921 tgccagaaat gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt
    16981 ttgactcaat cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac
    17041 cagtttacga aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata
    17101 agtctttagg ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg
    17161 gcgaaaaaat cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag
    17221 ctgcctctgc tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga
    17281 ctccgtttgg ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac
    17341 gcaaaggtca ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa
    17401 tcttagaatc gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg
    17461 aacaaaatga cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat
    17521 aaaacagtat aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa
    17581 agacgattgg gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta
    17641 tcactgtcac ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga
    17701 agcttggtta ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga
    17761 agaaaaaatc acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt
    17821 accaaaaatt atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt
    17881 cggtattgac ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga
    17941 aattttggca gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt
    18001 gatttgtaca acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga
    18061 agaaccagat ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa
    18121 agaaggattt aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac
    18181 ctatcaagaa ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg
    18241 tcgtgtttcc gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt
    18301 agaagagatt tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc
    18361 ttatcgtaca gaaacactga tacatttgac ccacttctat cattctcata attggacgat
    18421 gcagctgcac atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc
    18481 cgataccggc tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt
    18541 gaaccgtgca gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa
    18601 tgactatcct gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact
    18661 gcagttaggt tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac
    18721 acaatttgct gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag
    18781 tttcctgtcc tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga
    18841 aatggtcatg cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc
    18901 gattagttat acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa
    18961 cagtaacgaa tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag
    19021 aagaagacgg accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata
    19081 aacctttatg aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg
    19141 gcagaaaaag atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat
    19201 ttttcacccg agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt
    19261 atccaggaag gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt
    19321 gtacaatttg gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt
    19381 agctatttaa aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg
    19441 gtcttactga catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat
    19501 atacttggag actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt
    19561 ttagatgtgt tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa
    19621 aaattctacc tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac
    19681 aatacccact ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata
    19741 aaagaatccc gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt
    19801 aaatttaggc atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa
    19861 agtatcacag cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca
    19921 acttaaatta actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa
    19981 aaacaatatc gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga
    20041 agttcgtgca caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca
    20101 agccaagatg attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt
    20161 tactgaagaa gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga
    20221 aaattttggc gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca
    20281 gttagctaaa cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg
    20341 tgcgaatttg atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt
    20401 ggacaattta catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg
    20461 aaagatccgc atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta
    20521 tatcaaatat gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt
    20581 tcctgaagca atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg
    20641 aggaacgaat acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc
    20701 atctttatca gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac
    20761 atcttccatc ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga
    20821 aagattcttc tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg
    20881 gtcaaaatat attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag
    20941 tagaagctgg actaagagcg acccttatca agaaaatcca agaactatct attccttatc
    21001 aaaaagaatt gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc
    21061 aaacgttgtc ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag
    21121 ctttaggtat cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc
    21181 ctctcgccgc attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact
    21241 tcaggcaaga aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga
    21301 ttgctcgagc tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc
    21361 aaatctatga aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct
    21421 gggctagttt tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac
    21481 aagggcgcat ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca
    21541 attccgtttc tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca
    21601 cttctattgg cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa
    21661 tccaacattt ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac
    21721 tcaaaccgat tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg
    21781 ttgggccatc tggctctgga aaatctacac tccttaactt attgattgga tttatccagc
    21841 cgacttcagg aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg
    21901 tgcgaaatta tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag
    21961 agaacatcac ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag
    22021 ctgcgcaact ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg
    22081 aacatggcaa caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga
    22141 tacgtcagcc aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag
    22201 agaaaaaaat ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg
    22261 ctcatcggct ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa
    22321 tcgttgaaat aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga
    22381 aaaatgaatg acaaaaaata ggagctatga caagactttt gtcgtagctc ctatttctga
    22441 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcat caaacatgag
    22501 aagctgtttt ttgaaattgt tccttgtaac tcagagttaa acggtaggct cacgacctct
    22561 tggtaaatgg tgttcaagag acagcctatc ggcaataaga agcaattttg tcatcgctca
    22621 aggccgaacg tctctttcac accctctgtt gtttcataaa atagttttat tcgctgtaaa
    22681 ggtcgcgaac tggtgtcatg atgatacggt tcaagtcatt gatgattgta ctgaagcctt
    22741 gctctttttg catcaagtca ttgattactt cttctgattg gattttcaca gccatttctt
    22801 gtgctttcat tgcatcttcg tcgctgaact cttgtccact catttgtttt tcttgtaatt
    22861 cgatttgcat cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt
    22921 tctcgtacgc ttctttcaac gctttgaatt ctggtaattc acggatctca cgttcgattt
    22981 gattcgcgct atcgtagatg tttgacatgt tatgccctct tttcttttag tagttactct
    23041 ctattgtaac atggggttat tcgttttcaa aggggctact ctccttgata tttccgccag
    23101 aggtctttag ccgcttcacc aattccctga acgccttctt tcaccatatc gccgaagttt
    23161 ttcagccctt cttttgcttg ttcaccatat tgattcaagt tatcttgcca atttccagac
    23221 tctccattag aatttcctgt gtttccaact tgatctgcag ctaccacttg tcctcctgtg
    23281 gcataggcat ctgctacact aaatcctgtt tttgaagtat acggaaggat gccttctgcc
    23341 gctgatttga agactttccc gacaacattt ccacttgtcc cttccaaata gtgcagcttg
    23401 ctggattctt cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta accgatcatc
    23461 cattggtcat ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt tttccctgct
    23521 atcgtataac ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt accaagaagc
    23581 atgctggtca tttcatcggc tgtttccttt gatatgacct gttttggttt tttgtttgtg
    23641 ttatcgacga tcaccgctcc agtcgaatca acgattttcg tgataagatg cggcgtatac
    23701 atctgaccat cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg agatactcct
    23761 ttttccagtc caccaagggc taagccataa tacttatcag attttgctaa aggcaggccg
    23821 aattcttctg ctttatcata tcctttttgt aaaccgatct catgcaaaag ccatacagta
    23881 ggcaaattca aactttgtgc aacagcttga tacatcggta cttctccttg gtatgtcccg
    23941 tcaaaattat gcgcatcgta ataactttgc ggttcgtctt tcaagatact ggaaggcttg
    24001 tatccagctt ccagtgctgg tgtatatacg cttaatggtt tgatcgttga tcctggtgaa
    24061 cgacgcatct gtgtagcaaa gctgaatcct ctaaagacat gatctcctct tcgtcctact
    24121 agtgcttgta caccacctgt attcggatct aaagctactg aagcagattg gaccatggca
    24181 ccatctgaag catttgctgg gaataatgca ctgttttgat aagtagcttc taattgcttt
    24241 tgataatcct gatctaaagc tgtatagatt ttatagcctt tattcatgac atcttcttca
    24301 tcaagaccat actcgttaac tgcttcatcg atcactgcat caaaataata aggataacga
    24361 taaccagaat ctgaattatc gtatgtgtca ttaagatacg cgctgatatc tgtctggctt
    24421 tctgcttgtt cttgttcttt tgtgattttt ccgttttctt ccattacact tagtactgtg
    24481 tttttccgat cattcgcatt ttttgggtga tcgatcggat tataaatacc aggacctttc
    24541 agcattcctg ccaaagtagc agcctcagac agcgtaacat tcgttgcatc tactccaaaa
    24601 tactttctgg aagcgtcttg gacaccccat accccgttgc caaaataggc attgttaaga
    24661 tacatggtca agatttcttc tttgctgtac ttcttttcga tctcgattgc caaaaataat
    24721 tccttcgctt ttcgatcaaa cgtttggtct aatgtcagat acgcattttt ggcaagctgc
    24781 tgagtgatcg tacttcctcc gccccctgat gttccgccag acaagaccat tcggactgct
    24841 gcacgggcaa tccctttgat atcaaacccg tgatgctcgt aaaaattcct atcttctgtc
    24901 gagattactg catcttggat atagggagaa atcgcgttta attctacata tgttcctttt
    24961 tgggcgtaca gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt cgattggctt
    25021 aatcctgatt ttaacgtttc gacatttgct tgttttgcta aaataaagag gtagatactt
    25081 gtcactaaga cacagatcaa accaatcagc aaaatcaatt tgttcacatg atattttttc
    25141 cagatgcgct ttcgccatcg atggaattgt ccgagatatg gtctgatcca acgccaaaaa
    25201 ctgatcaaag cagcttttat cttttctaaa atgctttgaa aatccatttg cttcttctcc
    25261 tgtctatttg attcaatcgt cttattttac catagtgaaa gagtgttttc ttcctgctta
    25321 agttgtagtt caatatgttt ttttacgtta gtgcaagtag agtaacataa gaatgaacag
    25381 aaaaatgtaa gttttttctt aaatatgttt taaaaatggt acaattagtc tgcgattgca
    25441 ctgaaaggag tacacttgtg aaaatcacga tcactttacc tatgaaccat cctgaaacaa
    25501 caattcgcga cttgcttgaa aaggaatggt tagttcctag aaaggttcga cactttttgc
    25561 gtatacgtaa aaacgtttgg atcaatcaac aacctgcttt gttccatcat gaagtaaagg
    25621 gcaatgacca gatcacattg ttattcgaag aaacagatta taattatcaa gaagttcaat
    25681 taggagatgc gaagcacatc cgtgttttat atgaagatga gcatttgatt atagtggata
    25741 agcctgcagg aatgaagact catccaaatg aaccgacaga aaacagtacc ttactcaatc
    25801 atttagctgc ttatctcgac actaaacagc aacgacctta cgttgtccac cgcttggaca
    25861 aagaaacaag cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg atacttggaa
    25921 gaatgttgga acaaaaaatg atctatcgga gatatcaagc catcgtatgg ggagaaattg
    25981 ccaaagatat gaccatcacc aataaaattg gacgagatcg ccatgatcgg agaaaaaggg
    26041 tcgtagatga taaaaatggt cagagcgcct tgacacatgt cactgtcaac aaagtacttt
    26101 ctgaacaaac acaagtctat tgtgttttgg aaaccggacg tacacaccag atcagagttc
    26161 atttagctca taagggacat ccgatcgttg gagaccctct ctaccaaaat agaacagctg
    26221 aacgactgat gctccatgca ttagaactgc acatgatcca tccatttact caagaaaaaa
    26281 tagtcggtaa agctttacct ggattatggg aatagaaata aaaaagaaca gagtcttatc
    26341 agcagataat gataagactc tgttcttttt gtgtaatttg tctgcttact tttgatgttc
    26401 gtgttctgct aacagcaaac taccgataat cccactttca ttcggcagtc cccaacgaac
    26461 gatatattca cttacttgag gtgtttccat ataaccaccc atcaattcaa caaattgctg
    26521 acggattttt tgaaggagat gatcctgatt cattacccca ccgcctaaaa taatcttttc
    26581 aggtgctaac gtcaacgtat agttgatcag tgcttgagca atataataag cctgaatagc
    26641 ccaaacagga tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg cctcaataga
    26701 cggtcctgct gctaatcctt ctaaacaatc tttatgataa ggacaagtac cttcataatg
    26761 gtcatctgga tggcgtttca cccaaatatg tcccatttca ggatgtgcga tccctgaaaa
    26821 aatttcttca ttgatcacga cacctgcacc gattcctgtt cccacggtaa ggtaaataca
    26881 actatttttt ccttgtgctg ctccttgctt caattcccca tatgcagcag cattgacatc
    26941 cgttgtccaa gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa aatcgaattg
    27001 tgcccaccct tttttgggag ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat
    27061 accaatcggg ccgaatgagc caatcccgat tcccttcaat tcataacgat caaaaaattc
    27121 aaatacctga tccaatgttt cttctggtgt tgtggtaggg atacttaatt
//
LOCUS       DBKOIJ010000021        26240 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000021 DBKOIJ010000000
VERSION     DBKOIJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.021"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            105..995
                     /locus_tag="ACV5NJ_000695"
     CDS             105..995
                     /locus_tag="ACV5NJ_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HHY6484811.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1320..3791
                     /locus_tag="ACV5NJ_000696"
     CDS             1320..3791
                     /locus_tag="ACV5NJ_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HHY6484812.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3894..5630)
                     /locus_tag="ACV5NJ_000697"
     CDS             complement(3894..5630)
                     /locus_tag="ACV5NJ_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHY6484813.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5627..6331)
                     /locus_tag="ACV5NJ_000698"
     CDS             complement(5627..6331)
                     /locus_tag="ACV5NJ_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHY6484814.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6462..6965
                     /locus_tag="ACV5NJ_000699"
     CDS             6462..6965
                     /locus_tag="ACV5NJ_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HHY6484815.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6925..7263
                     /locus_tag="ACV5NJ_000700"
     CDS             6925..7263
                     /locus_tag="ACV5NJ_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HHY6484816.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7284..8702
                     /gene="ffh"
                     /locus_tag="ACV5NJ_000701"
     CDS             7284..8702
                     /gene="ffh"
                     /locus_tag="ACV5NJ_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HHY6484817.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8814..9392
                     /locus_tag="ACV5NJ_000702"
     CDS             8814..9392
                     /locus_tag="ACV5NJ_000702"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HHY6484818.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9396..9917
                     /locus_tag="ACV5NJ_000703"
     CDS             9396..9917
                     /locus_tag="ACV5NJ_000703"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HHY6484819.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9996..10550)
                     /locus_tag="ACV5NJ_000704"
     CDS             complement(9996..10550)
                     /locus_tag="ACV5NJ_000704"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HHY6484820.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10803..11078
                     /gene="rpsP"
                     /locus_tag="ACV5NJ_000705"
     CDS             10803..11078
                     /gene="rpsP"
                     /locus_tag="ACV5NJ_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HHY6484821.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11090..11341
                     /locus_tag="ACV5NJ_000706"
     CDS             11090..11341
                     /locus_tag="ACV5NJ_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HHY6484822.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11466..12257
                     /locus_tag="ACV5NJ_000707"
     CDS             11466..12257
                     /locus_tag="ACV5NJ_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484823.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12427..12948
                     /gene="rimM"
                     /locus_tag="ACV5NJ_000708"
     CDS             12427..12948
                     /gene="rimM"
                     /locus_tag="ACV5NJ_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HHY6484824.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12938..13699
                     /gene="trmD"
                     /locus_tag="ACV5NJ_000709"
     CDS             12938..13699
                     /gene="trmD"
                     /locus_tag="ACV5NJ_000709"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HHY6484825.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13835..14182
                     /gene="rplS"
                     /locus_tag="ACV5NJ_000710"
     CDS             13835..14182
                     /gene="rplS"
                     /locus_tag="ACV5NJ_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HHY6484826.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14555..15217
                     /locus_tag="ACV5NJ_000711"
     CDS             14555..15217
                     /locus_tag="ACV5NJ_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484827.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            15296..18523
                     /gene="fss3"
                     /locus_tag="ACV5NJ_000712"
     CDS             15296..18523
                     /gene="fss3"
                     /locus_tag="ACV5NJ_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HHY6484828.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18637..18951
                     /locus_tag="ACV5NJ_000713"
     CDS             18637..18951
                     /locus_tag="ACV5NJ_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HHY6484829.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18964..19338
                     /locus_tag="ACV5NJ_000714"
     CDS             18964..19338
                     /locus_tag="ACV5NJ_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HHY6484830.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            19348..19692
                     /locus_tag="ACV5NJ_000715"
     CDS             19348..19692
                     /locus_tag="ACV5NJ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HHY6484831.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19763..21115
                     /locus_tag="ACV5NJ_000716"
     CDS             19763..21115
                     /locus_tag="ACV5NJ_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HHY6484832.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            21175..22317
                     /locus_tag="ACV5NJ_000717"
     CDS             21175..22317
                     /locus_tag="ACV5NJ_000717"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HHY6484833.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            22342..22596
                     /locus_tag="ACV5NJ_000718"
     CDS             22342..22596
                     /locus_tag="ACV5NJ_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484834.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22852..24036
                     /gene="mobT"
                     /locus_tag="ACV5NJ_000719"
     CDS             22852..24036
                     /gene="mobT"
                     /locus_tag="ACV5NJ_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HHY6484835.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            24033..24170
                     /locus_tag="ACV5NJ_000720"
     CDS             24033..24170
                     /locus_tag="ACV5NJ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HHY6484836.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            24175..24396
                     /locus_tag="ACV5NJ_000721"
     CDS             24175..24396
                     /locus_tag="ACV5NJ_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484837.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            24409..24909
                     /locus_tag="ACV5NJ_000722"
     CDS             24409..24909
                     /locus_tag="ACV5NJ_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HHY6484838.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            25006..>26240
                     /locus_tag="ACV5NJ_000723"
     CDS             25006..>26240
                     /locus_tag="ACV5NJ_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HHY6484839.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMIS"
BASE COUNT         8725 a         4589 c         5672 g         7254 t
ORIGIN      
        1 tttacatgat ttttacataa cttaactata tttttacagc aaccatacga gggatttaca
       61 gtcgactgat aaactttagt tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt
      121 ttactctagg tatgatggga attcttttag ccggatgcgg ctcaggatca ggaaatacag
      181 atggctctgc cacacaaaat aattcaagtg actcaaatga acaagtaaaa atcgttgcgg
      241 taggttcgac agcattacag ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa
      301 atccaaatta tcaaatctct gtacaaggag gaggaagcgg aactggtttg agccaagtcg
      361 aagctggagc agttactatc ggaaactctg atgtatttgc agaagaaaga gacggagttg
      421 atgcttcaaa attagtggat cataaagtag cagtggtcgg gatggcgcca atcgtcaata
      481 aagatacaga tgtcaaagat atcaccaaac aggaattgat tgatatcttt acaggaaaaa
      541 tcactaactg gaaggaagtt ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa
      601 acggcagcgg tacccgcgcg acattcgaaa aatggggact ggatggtgct acccctgtgc
      661 agtcccaaga acaagattca tcaggaacag ttcggcagct ggtaagtcaa acaccaggag
      721 caatcagcta tttagctttc tcatatctcg atgattccac acaagcacta agtatcgacg
      781 gtgtagaacc aaaagaagag aatgtagcag ataacagctg gggaatctgg tcatatgaac
      841 atatgtacac gaacggaaaa ccttcccctg aagtccaaaa attcttagac tacatgatga
      901 cagaagaaat ccaagaagga cctgtcaaag aattgggcta tttaccaatc acaatgatgg
      961 aagtagaacg tgatcacgaa ggaaatatca aataactagc agataaacat gccacggtag
     1021 tcgttactat taaggaaaaa tcaactatgt ttcccacata tacatttttc ctttaattgt
     1081 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattaa
     1141 acaccgttta ttcgaaaaaa agagctacga catagaactg tcgtagctct ttttttgcta
     1201 tatgacagga atgaaaggtg tttgcaagct taatgtgagg aacactttcc atatctgttc
     1261 tatactggat aggagaagtt tggaaatatc cagaaaacga aatcaatcga ggtagccaca
     1321 tgaagaataa atggataaat cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata
     1381 aagtcatccg tctttttcca tcgtataaag aaggattaac ggcagatcaa gtcaaggaac
     1441 gaatagaaaa aggtgcagca aataattcag tcgatcccac attcaaaaca aatcagcaaa
     1501 tcgtattgga aaacatattt acgtatttca atttgatctt tcttatctta gccattttgc
     1561 tttgtttagt agaatcctat aagaacttga cttttttacc agttatcata gccaatacag
     1621 gaatcgccat ttttcaggaa attcgatcca aaaagatctt ggacgaacta aatgttttaa
     1681 atacagcaaa agtcaatgtc atacgggacg ggatagaaca aacaatagat atagaagacc
     1741 ttgtaataga tgacattgtt attttagaga ctggacatca aattccagca gatgcagagg
     1801 tcgtggaagg aaaactgcaa gttaatgagg ctttattgac aggcgaagca gatgaaataa
     1861 ccaaagaaat aggcgatcca ttaatgtccg gcagtttcat tgtttccgga aaagcttacg
     1921 cccgattaga caaagttgga gcagattcgt atatttccca gctgacagtc aaagccaaga
     1981 caatgggaga aggagaacag tcggagatga ttgcttctct taatcaattg atcaaatgga
     2041 ttgggatcat cattattcct atcggtatcc tgcttttttc tcaaagttac ttttttaatg
     2101 gaaacacgat caaagaaagt gtcgttgcaa tggaagcagc attgatcggt atgatacctg
     2161 aaggactgta cttgctgacg acgattgcat tagcattaag tgcgactaaa ctagcaaagc
     2221 aaagagttct tttacacaat atgaaaagca ttgagacatt ggcaagggta aatgtccttt
     2281 gtgtagataa aacaggaacg atcacagaaa ataaaatgtc tgttcaaaaa gtgatcgtct
     2341 ctaaaaaaca agaaataatc agtcagtcag atcaattgga agcatgcatt gctgactatg
     2401 caaaagcgat ggcaggagac aatgcaacaa tggaagctgt taaagcatat tttacaaaaa
     2461 ccactgatca atcctatcac catgttattc ctttttcttc tgtccaaaaa ttcagcagtg
     2521 tagcgttggc agacaaggtg tatgttttag gagcgcccga aatggttttg cgcgatcaat
     2581 tgccggaata tgcggaagag tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct
     2641 ttggtgtgta cgatgggatt cttgaagaac cagtattgac agaagcagtc acaccattag
     2701 gctatattct gattgctaat cctatcagaa aagaagcaag agccacattt gattacttca
     2761 aaaaacagca agtagccatc aaagtgattt ctggagataa tccgctcacc gtatccaatg
     2821 tagctgtcca agcagggatt tcacatgctg atcagtacgt tgatgcatct aaattatcag
     2881 aagaagaata tgaagcagct gtcgaaaaat atacagtttt tggacgcgtc aagccagaac
     2941 aaaagaaaat cttcgttcaa ttgataaagc gtaacaatac agtagctatg acaggtgatg
     3001 gcgtgaatga tattctagcc atgaaagaag cagactgcag tatagcgatg gcttccggaa
     3061 atgaagccgc catgcaggcc tcacaagtgg tcttacttga ttctgacttt tcacgcatgc
     3121 cagaagttgt tgcagaagga cggcgcgttg tgaacaatat tgagcgatca gccagcttgt
     3181 ttttagtgaa gaatattttc tcctttttgc tctcactgtt ttcagtgatc tttgctctga
     3241 cctatccttt agaaccatca caaatcacat tgattagttt gtttactatc ggacttccat
     3301 ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag agggaaattc atcatgaatg
     3361 tgatggaaaa agctgtgcca ggtggactaa cagatatgat cgtagtcggt gcgttggtga
     3421 tctgcggggt gactttgaat ctaaataaaa cagatgtttc tacagcatct accatgcttt
     3481 tgattgcagt agggttcctc gttctctata aaatctgttc accgctgaat aaattcagaa
     3541 gtcagatcat tcttttctgt gctagcggta actttttttc ggtcatcttt ttgcataagc
     3601 tgttttcaat caccggtatc tcagctgtat cacttttgct gttgagcatt ctatttttct
     3661 cggcagattc gatttttagg catttgacca cattggtaga atatttgttt gaacgtaaaa
     3721 atgaaaaaga gcaaaagccg tttagtcgcg tagctaaaaa atttctatcg atatttaaac
     3781 gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct
     3841 ttcgaagcag gatgagtcat ccaactttaa aacgcacgag cagttgctct tttttacatt
     3901 ttcggaatgg taacggtgaa agtcgaacct gttccaagat gactgtctac agtaatcgta
     3961 ccattaagga gttgcacgta gtcttttaca atcgctaacc ctaatccggt cccgcctgaa
     4021 tgtcggcttc gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc ctgatcctca
     4081 atgtcgatac ctataccaaa gtcttggaca gaaaacgata gatcatctgt tgcctgatag
     4141 cggataataa tctcgctgtc gctttttgaa tattggacag cattttcaat cagatttttg
     4201 ataattggat aaaggatatc tgttttagta gtaaatggtt cgttctttcc aatcaatcga
     4261 atcgtcaacc gtttcttttc gatgattggc tgataatttt gaacgatttg ctggaaataa
     4321 ggttctgtat aaattgtctg gatctcgtaa gaattctcgc catcttttga cagctggatg
     4381 atttcccgaa tcagtttatc taaccggatc gcatcttttt gcatgatctc aagaaaagaa
     4441 gtcagagttt gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa accaatcagt
     4501 gaagtcacag gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt
     4561 tctaaacgtc gcactttcgt taagtcatac gccaccccta gaatctgtcc ggtaccttct
     4621 gaattagtaa agtagcgcaa actcaagtct aatgtctgcc gaccttctgt aagggtgatc
     4681 tcctgatgga tcagcggatg ctcaggagtc acttgatgaa tcagctgaat gagttttggc
     4741 tcttgaatga cttctgtgta ccgctgttca ggttgatagc ttaccacgtt taaatgtagc
     4801 tgcatctttg gattaagcag tagtaaacga gaatcagcat catcaatcag gaatacgcca
     4861 atcatcagtt cgttcagtaa cgtatataat tgctcttctg tggtagtaca cgcccgatac
     4921 atcttactca tttgttcgct caacgcatta atcgtctgat acaattcttc ccattgatcg
     4981 ggtgtttgca tgattacttc cgcctgatta ggattttgca ccattttttt tagtacgggc
     5041 agcacggttt tcaatggctg gtttttttga tagatcagat aatagatcat tcctgcaaga
     5101 acaaggaaga aagacagaaa gaagaaaaag acccaacggc gaaaactctc agttctcggt
     5161 aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc actttttttg
     5221 actggtaatg cgacatataa aagatcctca ttcaaagtag tacttttgcg caaagcgcta
     5281 cctaggttcc caccattcaa tacggctttg atttcgggac gggagtttcg ctgttcattc
     5341 aatgtttcat tgtttgtgtc aaataagatc tttccagtag catccattaa agtaatccgt
     5401 tcgttcgact gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt
     5461 tcgacatcta attgccggat cagcagttgt gctttttttg tcaggtagct ttcttgctgt
     5521 tccacgatct gctgttgaaa ataatgggaa atcaattgcc agccaccaaa aaacaaacta
     5581 actaaaagaa taagaaagaa gagacgttca taaatttttt tccatttcat cgttttggtt
     5641 cctgaaactt atagccaaat cctctaatag tcaccaagta ctttggatgt tttggatctc
     5701 gctcgatttt ttctcgcaaa tgactaacgt gtacatctac gattcggctt tgtcctgcga
     5761 agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt
     5821 tcatgaaata aacaagtaat tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta
     5881 cggtcacttg ataattggtc aaatcagcag tgatttgtcc aatcgataga tattcaggct
     5941 cagcttcatc ttttgattgc ttacgaggtt caatccgtct gaatattgct ttcatacggg
     6001 ctaatacttc gcgaggacta aatggtttgg tcaaatagtc gtcagcacct atttctaggc
     6061 caatgatccg atccacttgg tcatctttag ccgtcagcat caaaatcggt gtttcatttt
     6121 tttcttgacg cagccgctga gtgatctcca tgccatccat cgttggaagc atcacatcta
     6181 aaatgatgaa atcaaatgat tgttctaatg ctaattctaa tccttcgcct ccatctgtgg
     6241 cactggttac cttgtagcct tctttttcca aattgaacgt caagagcgtt acaatagaag
     6301 gttcatcatc aacaactagt atttttttca ttcaaatctc ctttaaatag gtttcattct
     6361 gtttatattt tagcatagaa tcagtacaaa gggctatttt acaaaagaaa taaatggtat
     6421 aataggaagg cactttgctg aatagaaagg aagagcagtc aatgattgga atcagtgcat
     6481 gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa agaagtacca gaattgatgg
     6541 aactagttga aactggagaa gctgtcatgg tctgtccaga agtgatcggc ggattaccaa
     6601 ttcctagaga tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg aaagatcaag
     6661 caaaagtatg gacgacaagc ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca
     6721 aagcctatca aaaattgcag gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc
     6781 cttcatgcgg aacccattcg atttatgacg gaagtttttc cggaataaaa aaaaatggtg
     6841 ccggagtggc tactgcttat tttattcagc aaaaaatgac tgtgctctct gatgaagaat
     6901 ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc
     6961 tttgaatttt attcgacctt attgacagaa aaacaaatga actacatgga attatattat
     7021 gcagatgatt tttcattagg tgagatcgcg gaagaatacg aagtaagccg acaagcagta
     7081 tatgacaata tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa actccatctt
     7141 ttttctaatt atattgttcg agaacaggta ttggaaaata tgactgcata tattacagat
     7201 aagtacccgg aagataaaaa actacttgaa tatgtacagc aaattcaagc aatcgaagaa
     7261 taaataaaag gaatgaagaa aatatggcat ttgaaagctt aactgaacgt ctccagcagg
     7321 cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc
     7381 gggaaatccg ccttgcctta ctagaagccg atgttaactt gcaagtagtc aaagatttta
     7441 ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct ggaaagttta tcacctgcac
     7501 aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac acttggttct gaaacagcag
     7561 agttaaataa atcaccaaaa ataccaactg ttatcatgat ggctggtctt caaggggcag
     7621 gtaaaacgac ctttactggt aaattagcca attatttaaa gaaaaatgaa aatgctcgtc
     7681 cgctcctaat cgcaggggac gtatatcgac cagctgcgat cgaccaattg aaagtattag
     7741 gtcaacagct agacgttcct gtatttgata tgggaactga agtcagcccg gtagagatcg
     7801 tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg
     7861 caggacgtct gcatatcgat gaaacattga tggatgagtt gaaacaaatc aaagaattga
     7921 cgcaaccaaa tgagattttg cttgttgtcg atgccatgac tggtcaagat gccgtcaacg
     7981 tagctgatag cttcaaccag caattaggta ttacaggggt cgtcatcact aaacttgatg
     8041 gggatactcg aggcggggca gcactatcga tccgctcagt gacaggcgca ccaatcaaat
     8101 tcatcggttc tggtgaaaaa ttgactgact tagaagtatt ccacccagat cgtatggcta
     8161 gccgtatcct tggtatgggg gatatgctga cattgattga aaaagcgcag caagactacg
     8221 acgagaaaaa agcggaagaa cttgcacaga aaatgcggga aaattcattc gatttcaatg
     8281 actttattga acagcttgat caagtgatgg gtatgggccc actggaagat ctaatcaaaa
     8341 tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg
     8401 ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc agaaagagca aatcctgatc
     8461 tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc tggaaacagt gttgttgaag
     8521 tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag
     8581 gggatatgaa catcccaggt atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag
     8641 gcaaaatggc gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat
     8701 aagatgaaaa aaacagcttc ggtacagact gaaaatgtct actgccggag cttttctatc
     8761 agcaattttt attctttact aatacaatta ctctaaacga agaggtggca cagatgagat
     8821 gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc tggggtgttc
     8881 ctgaatatga tgaccaaaag ctgttccgaa aattgatgct tgatattaat caagccggat
     8941 tgagctggca gacgatttta aataaaagtg aagcattcga tgaagcatat gatcgattcg
     9001 atatcaaaaa agtcgcaaat tatggagaaa caaaaatcag tgaactaatg gaaaacaaag
     9061 gaattattcg taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga
     9121 aaattcaaga agaaatcggc agtttttcag agtatctttg gtcgttttct aatggaaaaa
     9181 tcgttgattc ttcttatcat tcacaagaag aagtcccaac aacaagtcct ctttcagatg
     9241 aattagcaaa agatctaaag aaaagaggat ttaaatttat cggaagcaca acaatctatg
     9301 cttttttaga agctgtcggg atcatcaatg atcaccttga cagttgtttc cgcaaagaag
     9361 aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg aaaaatcata ttttatggtg
     9421 ctataagttt agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca
     9481 ctccaacagg taagcaaact acttatgaag ctttttatga aacaatcgat acgaccatca
     9541 tgggcagaaa aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag
     9601 agaaaaagaa tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg
     9661 taaacgaaga tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct
     9721 gggttgtggg tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat
     9781 ggtatatcca gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa
     9841 catatgaagc gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc
     9901 attatgtacg aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct
     9961 gtacatagtt gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa
    10021 tctagatacg catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca
    10081 ttttcatcaa ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc
    10141 acattcaaaa agacaagtga ttggcgtaga tggtggtttg cgccaaagcc gccgatccca
    10201 cctggtgatg cagtaacgac taaacctggt ttatgatccc aaacactgtg tccataaggt
    10261 cttgagccta catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga
    10321 gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca
    10381 ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca
    10441 aatacacttt cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg
    10501 taagaatctt ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc
    10561 ctccttcaaa ctatctataa taatcttaca aaagataagt aactggagca aataaaaaca
    10621 cttactgaca aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa
    10681 aatcttctat tttctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac
    10741 agggtaaaaa aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa
    10801 taatggcagt taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta
    10861 tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt
    10921 acaatccttt gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt
    10981 tatcaaaagg tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta
    11041 tgcaaaaaca ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt
    11101 aagcgactta gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt
    11161 agacgtcgta gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat
    11221 tggccgtatc attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg
    11281 tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta
    11341 aataaacgtg ctgtactcga attttcgagt gcagcttttt tgtttttttt attaattgaa
    11401 gaaatgatta tttggcaaaa caagcaagaa agcgtatcct tataggagga gggagtgaac
    11461 agccaatgag aaagaaaatg ttatttattc catttgctct attgttaagc gcttgtagtt
    11521 cggttactca agaatcagac aatacacatc tatcaagcag tatatctgaa actgttacca
    11581 gtccagaaaa aagtagtacc aaaacggctt ccaccactga acaaacaatg gctacctcaa
    11641 ataatgagaa aaagactgct ttagaccagt tgaaagaaca acagccaaat gttcccatgc
    11701 ctctagatgt accagtctct tcaggctatc tcaatatcgc agcgacacat acaaagcaag
    11761 gttattctat tttatattac agaacagatc gtcccctcgg gttgaatgct gacgaattga
    11821 atcaagaaac gcctattgca acctatctat atcaatatgg gtttgcttct agtcaagaaa
    11881 caatccaagt tttgcagcca tttgagatcg acacaaatgg tcagcaagtg gacttaggtt
    11941 ctcgaatcac agggtatcag caaggtgcag caggttcaag ttttcttgaa tggcaagaag
    12001 gaaactggcg tattcgaatc cgtgggaata atatagaagg ccaagatccc ttactattag
    12061 ctaaagaaat cgtagcttat ttggaagaaa acagtctacc agcacctgaa caatttggta
    12121 aaattactgt tgatatgggt gatacgacga atcgagccgt agaagtaagc tggcaagaac
    12181 cgaaaaatgc ctatacgatc actcatcaag atcctatgtc tgcgttgaag atggcagttt
    12241 ctatgaagag gttgtgaaat cagcgcgctg atctgagccc atcatttaac tccaagtcac
    12301 aggaagtaat caaaaatagc acgccgcatt tttgcataat tttggcttaa taccttaaaa
    12361 gaaaactaga attgcctctt cacacatgct aaaataaaac agacaaaaaa gaaatgggga
    12421 tataagttga ctgaatactt gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc
    12481 gaagtccgtg ttatctcaac aacggatttc ccagaagaac gttacaaaaa aggaacggtg
    12541 ctaacactat tccaagacgg aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa
    12601 cataaaaatt ttgacttatt gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa
    12661 tatcgggatg gcatattaaa agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa
    12721 ttctattacc atgagatcat cggtctgcat gtattagaag atggaactga gttaggtaaa
    12781 gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa
    12841 gctgatatat tgcttccata tatcgcttct gtcgtcaaag aagtcgattt agaagctgga
    12901 gtggtccgtg tagaaattcc agaaggactg attgacgatg aagattgatg tattgacttt
    12961 atttccaaga atgtttgaag gtcctatggg ggaatccatc atcggtaagg ctgtagataa
    13021 aggattatta gatatcaata tctctaactt ccgcgattat tcagataaca aacatcagac
    13081 agtagatgat tatccatacg gcggtggagc aggtatgctg ctcaaagtcc agcccatcta
    13141 tgataacatc caagccattg aaaaagctgc accggatgtc aaaaaaagag taattcttct
    13201 tgacccagct ggtaaacgat tcgatcagaa aatggcagaa gaattttcac aagaagaaca
    13261 cttagtattc atttgtggtc attatgaggg atatgatgaa cgtatccgca cattagtgac
    13321 agatgaagtt tccttaggag attatgtcct tactggggga gaattaggtg cgatggtcat
    13381 gatcgatgca acagttcggc ttttgccaga tgtgttaggg aatcagactt cagctcaaac
    13441 ggattcttat tcaaccggtt tattggaaca tccccaatat actcgcccgg cagaatataa
    13501 agggatgaaa gtaccagaag tattgacaaa tggaaatcat aaattgattg aagaatggca
    13561 attaaaagaa tcactaagaa ggacctatca acgtcggcct gatcttttgg aaacacttga
    13621 atggacacct caaatgacga aatttttaga agaaatcaag aaagaagaac aagaaaaaac
    13681 agcagatgaa gccaagtgac tttttctaaa atcttcttta cagtaggttg ataatatgtt
    13741 agtattttat ggtgagtgca aaagcactta actattacga tattccgctg tgagaaaagc
    13801 tcataagaat atttggaata aggagaagaa aaccatgaat ccattaatcg aagaattaac
    13861 aaaagaacaa ttacgttctg acatcccagc gttccgccct ggtgacactg tgcgtgttca
    13921 cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa
    13981 acgccgcggt gctggaatca gcgaaactta tacagtacgt aaagtttcta acggagtggg
    14041 cgttgaacgt acattcccat tgcatacacc acgtgttgca aaaatcgaag tagttcgcta
    14101 cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa aagctgctcg
    14161 tatcaaagaa atcagaagat aaacacggaa aacttccttg gaaatctgaa atttgtgaaa
    14221 agtccttgta tatcaagggc tttttctttt ttctctatca cagttttaag ccatttttgg
    14281 ctgttttgga ccatttttca atcatattcc cttaaaaatt gtcctagatt gtccgacatt
    14341 gactatcagt gtcgtcaaaa tagggcaaaa tttagtagtc agcttgacga caaatataat
    14401 acgaaaaata ccaaaacatt taccgaattt agcaactttt gaacccaagt ttctcaaagg
    14461 gtcagctatt agcaaaaaag acaaaacatg tacttttcta ctaaaaagtt gatgtgatag
    14521 gcttctatct aaataactat taggagtttt tatcgtgaag caaaatgatg gacgtatcat
    14581 ttggaaaaat caaagtgagt taaaactgat tttaacaatt aatgaattca tcgaaaaaca
    14641 cggaattaca tctagccgtc aataccagaa aaagctatca gaaaatccaa attctgcacc
    14701 aagtatgtgg ttcattaaca aaaaatatgg atcatgggag aatttattga ttagtatagg
    14761 acgagaaaat actgggtatg gaaaatgggc tagaatgtca gaacaagaat tattggaaat
    14821 agtagaagcc tttattaagt gcgagaagat aacctctcaa cgaatgtacg aacaaaaatc
    14881 agtagggaag aatattcctt cacttagtac cattaaaaag atgttaggag atatacgtcc
    14941 actttttaaa gaaaaaaatg atggatctcg atttactgat ttcgaactgc tgttagaact
    15001 aaaaaatgaa ataatccgat taaaactaca ggatgattta tcaatgacaa agtttcgaaa
    15061 actagttcaa tccccaaaac taccatcagt tgatacaatt atgaaaagaa cgaacaaaaa
    15121 ttgggaggaa ttgatggcag agattggatt tgattatcga agaatcaaaa tttataagca
    15181 gagaaacaac ctatccaaga caaagaaaac taaatagtat cactctaaat cagacgttca
    15241 aaattaaaat ttggacgtct tttttattac ccaaaaacga aaggagattt attttatgaa
    15301 aaagccaaga tttaagaatt ggcgtttgct cgcaacactg agtctattat gccaaacaat
    15361 tggtggatcg cttggcccaa cgattgcctt tgctgatgaa attactcatc cacaaacggt
    15421 aactgtggaa ttggatttgg ctcaccagta tgctgtcgaa ggtacattta gcgatggtcg
    15481 tcccatgtca gaagttaccg ttccgcacta tgcagtttac aatggtgtga agcaagatat
    15541 tttctgtatt gagccagggg tgccgattta caatgagttc actcctggtt atgagaaaaa
    15601 tccattgcct gatatgtcgg agaaagcgaa attagtttcc gttttatgga aggaagctgg
    15661 tacggatgtg gatacacata ttgtggccca gaagatgatt tggcaagaag ttaatggcta
    15721 cacgcttcac tcaattaaac gttctgatgg tagtgcagta aatatcgcag ccattgagtc
    15781 aaaaatcaat caagccattg cggattatca aaagaaacca agcttccata actccacagt
    15841 gaaaaccgtg ttaggtcaat cgactactgt gacagatacg aacggcttga atttatcgga
    15901 gtttgatgaa gttgtcgaga atatcgcaaa tattgattat cgtgtgaacg gcaatcaact
    15961 agtgattact ccaaatgcca attcaaaaga gagtggtgaa ttaaccctaa agaagtcagc
    16021 taatacagga acaccggtag cttataaaat ggctggtcaa caaactttga tggcgggggc
    16081 aattgataag cccaatacct acacaatcaa aattgatgtg gaaactgagg gttctttgaa
    16141 gattaaaaaa gtcgataaag aaacaggaaa tgttgtaccg ggaaccgttt tccatttaga
    16201 ctttggaaaa acattacctg caaaagacgt gaccaccgat aaagaaggca ttgcgacatt
    16261 ggatgagatt cctcatggcg cgaaagtaac cattactgaa aaatcagtgc cagctcctta
    16321 tacgattgac actacaccaa tcaccgcaac cattaaatct ggtgagacta tttctgtgac
    16381 ttcaaaaaat actcgcgaaa aaggtcaaat cattttgaat aaaatggggg tggaaacagg
    16441 aactgatctt tggaacgaca aatactcatt agccggaaat acgtttgcca ttcgcaaaga
    16501 cagtccaacc ggtgaaattg tccaagaaat gaccacggat gaaaaaggtc atgcggaaac
    16561 accaaaagag attgccaatg cgttggaatt gggaacctat tatgtgaccg aaaccaaagc
    16621 tagtaatggc tttgtgaata cctttaaacc agtaaaagtc gaattgaact atgtcaatca
    16681 aaccgtggct cttgttacca gtaacgtaaa agggcaaaac caagaaatca ctggtgaaac
    16741 cactttgaca aaagaagaca aagataccgg taatgaaact caaggtaaag ctgagtttaa
    16801 aggagctgaa tatactctct ttactgcaaa agatggtcaa gcggtcaaat ggagtgaggc
    16861 ttttaaacca gagttagtga agggaacgaa agcttctgat gaaacggtga ctttgacttt
    16921 agatgaaaag aatcaagttg ctgttaaaca cttagcgatt aatgagtatt actggcaaga
    16981 aaccaaagca cctgaaggat ataccttgga tgaaacaaag taccctgttt ccatcaaaaa
    17041 ggtggatgat aacgaaaaaa atgccgtgat tacccgagat gttacggcaa aagaacaagt
    17101 cattcgcttt ggctttgatt tctttaaatt tgctggatca gctgctggga ctgccgaaac
    17161 tggcttcaat gatctgacct ttaaagtatc gccattggaa ggaaccagtg agattacaga
    17221 tgccgaagat gaagcaatta cagcttataa cgagcaacta ggttttgatg gctatggcaa
    17281 gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaggctc cagaaggatt
    17341 tcaaaaaatt acgccattag aaatccgttc tacttttaaa gaaaataaag cggattacac
    17401 taagagtgaa tacattttta cgattaccga aaaaggacaa aaacaaccaa ttaaaacggt
    17461 gaccgttcct tacgagaaac tgacgaataa agcattttct gttagcttga atcgtttgat
    17521 gctctatgat ttacctgaag aagaagatag cttaacttct cttgggactt ggaaagacgg
    17581 aggcaaagaa ttgacagacc ttgattctac cgagctagtt gataaattga gctataactt
    17641 gcatgaaatc aaagaagact ggtatgtggt agcgcaagcc attgatgtgg aagcgacaaa
    17701 agctgcccaa gaaaaagatg aaaaagccaa accagtggta attgccgaaa caaccgcaac
    17761 gttggcgaat aaagagaaaa caggaacttg gaaaattttg cataaattaa ccgcagaaca
    17821 agttttggat aaaaccatcg tcttattcaa ctatgtgtat gaaaacaaga aaccctatga
    17881 agcaggtgat gatccagtag cgaaagatgc tagcttgaac aatcaagccc aaactgtcaa
    17941 ttgtacggtg gaacgccatg tttccatcca aacaaaagcc cacctagaag atggttcgca
    18001 aacctttact catggggatg tggtggatat gttcgatgat gtgtcgatta cccatgatgt
    18061 actggatggc tcaaaagaag cttttgaaac aattctgtat gcattacttc cggataatac
    18121 gaccaaagaa atttggaaat ctggcaaaat tgattatgaa gtgaatgaca aagaattcac
    18181 taaaaccgta cttgcggaaa aagtggacac aagtaaatat ccagaaggta caagttttac
    18241 cttcaaagaa attaactatg gcaaagacgg aaacatcaat gggaaacaca atgaggatct
    18301 aaaagaaaaa actcaaactt taacacctaa aaaagttccg acaacaccaa gtgcaccaga
    18361 acaaccggaa ataccaactg ttccaagtga ctctcaagaa tctagtccta cagtaaagac
    18421 attcccacaa acaggggaga aaaattctaa tgttctgctg tttattgggt ttaccttgat
    18481 ctttgcgact gctgggtatt atttctggaa tcgccgaaac taaggtgatg tgatgaaaca
    18541 actaaatagg aaaaaccaac gtgcgcgacc gcctcctaaa aaaagtggtc gcgctatttt
    18601 tgtatccaaa actaaaaatg aaaagaggaa aagaagatgg aattgaaatt tgttgtgcca
    18661 gatatggccg aaacctttgg aaagataagg tatgccggcg aaggcgaagt tttaactgaa
    18721 ggatatgggc ggaatactac agtaattggt cgtagttatc atttgtattc cagtaagcaa
    18781 cgagccgatg atattgaagt ggtggtagct gcggaagccg gcgaaaagga ttttgatcaa
    18841 gatcaaccac taaaagccgt gaatccccat ttggttgcca aaggctatga gattgagaat
    18901 cgtgggttta ccgactacgt gttgtatgtc gatgatttag tgaaagcata ggaggaaaag
    18961 aagatgagat taacagaagg tattgttgta gattcaggac taacgtttgg gaaattacga
    19021 ttttccgcat tacgccgaga agtacggaag caaaatgagg atggaacggt tagtaacgag
    19081 gtaaaagaac ggacttataa tttgaaatcc tctgcgcaag gtcgaatgat ccaagttagt
    19141 atcccagcaa atgtaccctt acgcgagttt gcctatgacg cagaggtaga gttggtcaat
    19201 ccaatcatgg atacggtcgc caattatgtt ttccgagaag gaactactgt caattggttc
    19261 attaaggccg atgatttagt cttgaaacga caaccagatc agggaaattc tacaaaccaa
    19321 aatgaaggaa agaaataggt gaagcgtatg gaaattaatg ttgaacatat tttagattgt
    19381 ctcgatcaat atggaaaagg tgagctaacg gaggaacaac tcactaaagc attaacctat
    19441 gatgaaaaaa tgtttctgat tatgcatcaa gggttactgg aagtaggtaa cgatgccgaa
    19501 gaagattttg atgtactgaa ttggttaggg gaagaagagt ccttctttat ggttatcgaa
    19561 gtaaatgaaa gtctatgtcg agaagccgaa accgtattgg aagaaattgg tgtggaaatg
    19621 ccggatgcca ttgaagtttt tttaaagcaa ctagtggaaa cgaaacaact ccctatagca
    19681 gtaaatgact agcataactg cttgaaattg gatcaatgaa atcctatgaa gcttttttcg
    19741 attggaggtg gaaaccataa aaatgtacaa agggcatcgt atacgggcag gagatcagca
    19801 cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt ggatgagcgt
    19861 cttttatttt caagagctta gacaatttga tatttctaag ctatcacttt ctactataga
    19921 aatcgttcgt tctataaaag atttaatcta tttgctagga agtctcgttc tttcaggaag
    19981 cacgatgttg ctttatatac acttttttca ggatcattgg cgcagtttgt ggcatcgaca
    20041 aaagctagcc cggatgattc tagaaaatca ttggtacgaa gtaaaacaaa ctcagagtga
    20101 aggatttttc aaagacctca atagtagtcg aaccaaagag acaatcagtt acttccccaa
    20161 aatctattac cgtatgaagg atggtttact ttctattcgc gttcaaattt cactgggaaa
    20221 atatcaagat cagcttttaa agctggaaaa gaagttagaa agtggtctat attgtgaact
    20281 agtagaaaag gaactcaaag actcctatgt ggagtacact ttactgtatg atataattgc
    20341 caatcgaatt gggatagacg aagtagtggc agaaagtggg gctttgcgat tgatgaaaaa
    20401 tcaagtatgg gcttatgatt ctttacctca tatgttaatc gctggcggca caggtggtgg
    20461 gaagacttac tttttactca ctatcatcga ggctttatta aaatctgatg ccgagctttt
    20521 tgttcttgat cccaaaaatg cggacttagc cgatttaggt acggtaatgc ctcatgtcta
    20581 ttcccaaaag gaggaaattt ctgcttgtgt ggaagatttc tatgagcgca tgatgactcg
    20641 tagcaaggca atgaaagaaa tgcccaacta caaaacagga gagaattatg cgtttcttgg
    20701 acttccacca aactttttaa tctttgatga atacgtggtt tatatggaaa tgttaacgac
    20761 gaaggaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttag gtcgtcaatc
    20821 tggtttcttt ctgattctag cttgtcaaag accggatgcg aaatatttag gagacgggat
    20881 tcgcgatcaa tttaatttct gggtggcttt gggtcgtatg agtgaactgg gatattccat
    20941 gatgtttggc gaggttgata aaaaattctt tatgaagcgt atcaaaggtc gaggctatgt
    21001 agatacggga gggagtgtga ttagtgagtt ttatacgcca cttgtaccaa aaggatatga
    21061 tttcttaagg gaaattagcg acattggtgg attaaacgtt aatacggaag gataaaataa
    21121 tagtatgtag cctaggagtg tcaagaatcc tgggcttttt ttacgaggtg atttatgaca
    21181 tttttagatt tattcgctgg gattggtggc ttttgcttag gtatggaaca agcgggtcac
    21241 cagtgtatcg gcttttgtga aattgatgat tttgctcgcc agagttataa ggcaatccat
    21301 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagacga gtttattcaa
    21361 tctctcggac cagtggatat cctttgcggt ggatttccgt gccaagcttt ttcaattgcg
    21421 ggaaagcggc aaggattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
    21481 gccgctattc tccaacctaa gtttttattc cttgagaacg tccggggatt actcaatcac
    21541 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgatgtg
    21601 gaatggcaag tgcttaactc aaaggcctac gttccccaaa accgtgagcg gatcttcctt
    21661 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg atcgtctcca
    21721 acatctgatc aaaacatcag aaacttatta aatatcaacc cttccaatcg aggaatgggg
    21781 ggacaagtct atgggtcaga tgacgtagcg cctaccttga ccggtgatga aggaatcaaa
    21841 atcgcattgc cggtagatga tggaatttct gtagcgggta tgttactggg aaattttgaa
    21901 caaggcaatc gggtttatga gataactgga acagcgccta ctttgtcaac gaaacaaggc
    21961 ggaacgaaga tcatgcttcg gaaaaacgaa acagaatatc aagaggtgaa gccagggaac
    22021 agtgtgaacc tggcttttcc taattccaca agtcgtcgag gccgtctggg aaaacaaagt
    22081 gtgcatacct tattaacagg agaccaacaa gcagtcgtca cggatcagta tcaaattcga
    22141 aagttaacac ctagggagtg ctggcggctt cagggctttc cggactgggc ttttgatcga
    22201 gcttctcaag tcaattcaga tagtcagttg tataagcagg ctggaaattc tgtaacagtt
    22261 ccggtcattt ttgatattgc tagaagatta aaggaggtaa aaacagatga tttatgatga
    22321 gttgattggg gaatctactg ggtgattggc aagattcagt cagatccgga attggaagaa
    22381 gaactgtacc ggctcaattt tgatattcgc aaaaacgggg tgaaagtccc tgatgatccg
    22441 tatgtaattg atgaggaaac ggatgctcga atggagctga atcaagtcat cagtgaattg
    22501 gaacgaattg cggactttgc taaagaacct gatatccgcc aatacttgtt tgagataaaa
    22561 gcagagttgg aaattgaggg tattaccgcg gagtaacggc caaataaacc actgataggc
    22621 actgaccgcg ggggatttgc gaagcagaaa tcctccgtgg tgctgtgcct gtttatggac
    22681 ggcgttagcc ggccatgatg tctcaccccc cgtatctaac aggggggtac aaaaacacaa
    22741 aaaataagca agcaacgata aggaatggct gtcctatcaa ggataataag caatttgtgt
    22801 agatgttaac tgatttgaaa aagttaacac cttaaatttg gaggtgagaa cttggcacaa
    22861 aggaatttag attatcggct attaaaagac cggcgtaatg aatatggcgt ttcacaaaat
    22921 aaattagcta cgacttgtgg acttagtcgc ccgtacttaa atcagataga aaatggtggc
    22981 gtaaccgcat cgacaaaaac catgaggaaa atttttgacc aactggaaag tttcaatccc
    23041 gagttacctt taacgttact gtttgactat gtaaggattc gctttcccac aacggatgcg
    23101 aggaaaatca ttcaagagat tctccatttg aaatttgatt atatgctcca cgaagattac
    23161 gccttttact cctatcaaga acaatatgtt atgggagata ttgtggtgat gttgtcacat
    23221 gaagaagata aaggcgtcct tttagaatta aagggtcgtg gttgtcggca gtttgaaact
    23281 tttttactcg cccaaaagcg tagctggtat gactttttcg aagattgtct aaaagccggt
    23341 ggcgtgatga aacgcttaga cctggcaatc aatgatcgag ttggcctgtt agatattcct
    23401 gatttaacga aaaaatgtca gaaggaagaa tgtatctcct tgtttcgtac cttcaaaagc
    23461 tatcgttccg gggaactgtt gaaacctgat gataaagatg gtatgggcaa tactttgtat
    23521 attggcagtc ttaagagtga ggtctatttc tgtttgtacg aaaaagacta tgagcaatat
    23581 atcaagttag ggattccttt agaccagacc gaaacgaaaa atcgttttga aatccgactg
    23641 aaaaatgatc gagcttatca tgcaattcaa gatttgttaa aaggtcgcag tattgaaagt
    23701 accacatttt ccattattaa ccggtacttg cgatttgcgg ataaggtgga aggcaaaagg
    23761 cgaactaact ggcccttgaa tgaacaatgg gggcgcttca ttggacggaa tcagaaggaa
    23821 attcaattaa cttctgaacc gaagccttat accattgaac gaacgttgaa ctggcttgga
    23881 cgacaagtcg cccctacgtg gaagatggcc aaggagttgg atcggttgaa tcagacaacg
    23941 tacattcagg atatggtaaa taatactcga ctatcagacc ggcacaaaaa gattttggag
    24001 caacaaagta tggcaattga aaatttgatt atatgaaaag aggaatcgtg atggaaattg
    24061 tgaaagatat tttattggtg tttggtggca gtatgcttgg tgttactgtg atgtgcttga
    24121 tgcatgcaag tgcagcggcg gaccgagcaa tggaaaagaa aggaaagtga tgaaatgaat
    24181 ttcggtcaaa atttgtatca atggttttta accaatgctc aatctttagt cttactagcg
    24241 attgtggtca ttggtttgtt tttaggattt aaacgcgagt tttccaaact gattggcttc
    24301 ttggtgattg ctttgattgc ggtaggtctg gtctttaatg cttctggtgt gaaggatgtc
    24361 ttactcaatt tgtttaaccg aattgtcggt gcctgagggg gaatcacaat ggaacagata
    24421 cgtgtttata ttgcgaacct tggaaaatac aatgaaggtg agttggtggg ggcatggttt
    24481 accccacccg tcgattttga tgaagtgaaa gaacggattg gattaaatga cgaatatgaa
    24541 gagtacgcaa ttcatgatta tgaactgcct tttgagattg atgagtatac accaattgag
    24601 gaaattaatc ggttgtgtgg tttagcggaa gagcttaaag gaacgccaat tggtgaggtc
    24661 gcttcagaga ttcaacatgc cttcttcaat tctttcgagg aaatggtaga gcatgtggat
    24721 gatatcatct attatcccga ttgtaatgac atgagtgatg tagcgtatta cttgattgta
    24781 gaagctggtg atttaggcga agtaccagaa cacttgaaga cgtatattga ttttgatgcg
    24841 tgtggtcgag acttggagat tggcgggaat tatttggtta cgagtcgagg ggtttttgaa
    24901 ctaatgtaaa tcatttgtta gaattgacca gaaatttcta tcgtactttt acttttctat
    24961 ttgatatact gaaatagaaa atatttgttg ataggggtgt tactaatggt tggcaataag
    25021 tggtacaaat gtgatcttca tattcattca actgcgagtc agtgctttca ggataaaaag
    25081 gttactgcta aggaatgggt agaagaatgt ttaaacaaag gactcgattg tgttgcatta
    25141 actgatcaca atacagggga aaatattgat gaatataaga aagtggctat cgaaaatggc
    25201 ttagattttt ttcctggagt ggaattaacg tgtggagaaa atggaactca tttactagtc
    25261 ctatttgata aaagtgcatg tacaaatgat atctctgatt tcttaaccaa gatgggtata
    25321 aaaagagagg attgggccag cgctgcagct tgctcaatga aatcagtttt agaagttatt
    25381 gattttgctg agcacgaggg cttgctcgtt attcctgcac atatagatga gtttaatgga
    25441 atttcttcag caaaatttgg aaatctagga aagatatttg aatcagaaaa tatatatgca
    25501 gtacagacgg ttcaaaaaga attttttgaa aatcgctcac aaatgattcc ctcatctaag
    25561 cgtgacacta tctatgatag tgtgaatgcc agatatgatg gtagagttgg aaaagatact
    25621 ttggaatctt ggtataaatc agttgttgag gcagaaaaaa ataatatgac atttcttagc
    25681 ttttctgata acccacactc gaaaggaaat tcaaaacatg gactttgggg aataggcaca
    25741 agatattcct atataaaaat gaaggacgaa cctgacttgg gatcacttcg cgatgcttta
    25801 atgctaggcg aaactcgagt tcattctgat ttttctaatt tctccattaa tgaaaatgaa
    25861 gttttattgg aaaaattaag cttttctggg accactctta gcacaaaaga agttgtagtc
    25921 gaattcagcg ataatttaac ttcaattatt ggaggaagag gaacaggcaa atcttgtatt
    25981 acaagattct tggtctatgt ccttggaaag gaagcagaac ttgatcaatt ttctgaaata
    26041 caaagtgatt atcagaactt tgcacaaatt gaacataatg gctctggaat atttttgaag
    26101 gatacaattg tgaaactgaa tattttctat aaggggacaa aatatcaaat aataagaaca
    26161 caggatagac attctattta tgaatttaca caagagaatg gtctggttga aaaagaaaca
    26221 gaacggttga gaatgatttc
//
LOCUS       DBKOIJ010000022        25223 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000022 DBKOIJ010000000
VERSION     DBKOIJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.022"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(164..388)
                     /locus_tag="ACV5NJ_000724"
     CDS             complement(164..388)
                     /locus_tag="ACV5NJ_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HHY6484840.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACV5NJ_000725"
     CDS             complement(586..2166)
                     /locus_tag="ACV5NJ_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HHY6484841.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACV5NJ_000726"
     CDS             complement(2567..3943)
                     /locus_tag="ACV5NJ_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HHY6484842.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="ACV5NJ_000727"
     CDS             complement(4071..5189)
                     /locus_tag="ACV5NJ_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHY6484843.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACV5NJ_000728"
     CDS             5331..5765
                     /locus_tag="ACV5NJ_000728"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6484844.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /gene="ntdP"
                     /locus_tag="ACV5NJ_000729"
     CDS             complement(5843..6376)
                     /gene="ntdP"
                     /locus_tag="ACV5NJ_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017110 - nucleoside diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside tri-diphosphate phosphatase"
                     /protein_id="HHY6484845.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACV5NJ_000730"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACV5NJ_000730"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HHY6484846.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACV5NJ_000731"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACV5NJ_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HHY6484847.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACV5NJ_000732"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACV5NJ_000732"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HHY6484848.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACV5NJ_000733"
     CDS             complement(10101..10679)
                     /locus_tag="ACV5NJ_000733"
                     /EC_number="1.14.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase auxiliary
                     activity family 9 protein"
                     /protein_id="HHY6484849.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACV5NJ_000734"
     CDS             11001..11471
                     /locus_tag="ACV5NJ_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HHY6484850.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACV5NJ_000735"
     CDS             complement(11579..12805)
                     /locus_tag="ACV5NJ_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HHY6484851.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACV5NJ_000736"
     CDS             complement(13559..13759)
                     /locus_tag="ACV5NJ_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HHY6484852.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACV5NJ_000737"
     CDS             complement(13893..14501)
                     /locus_tag="ACV5NJ_000737"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HHY6484853.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACV5NJ_000738"
     CDS             complement(14586..15056)
                     /locus_tag="ACV5NJ_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HHY6484854.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACV5NJ_000739"
     CDS             complement(15104..16018)
                     /locus_tag="ACV5NJ_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HHY6484855.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACV5NJ_000740"
     CDS             16164..16967
                     /locus_tag="ACV5NJ_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HHY6484856.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACV5NJ_000741"
     CDS             17145..18092
                     /locus_tag="ACV5NJ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HHY6484857.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACV5NJ_000742"
     CDS             complement(18160..18453)
                     /locus_tag="ACV5NJ_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HHY6484858.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACV5NJ_000743"
     CDS             complement(18481..19146)
                     /locus_tag="ACV5NJ_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HHY6484859.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACV5NJ_000744"
     CDS             complement(19286..19918)
                     /locus_tag="ACV5NJ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HHY6484860.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACV5NJ_000745"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACV5NJ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HHY6484861.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACV5NJ_000746"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACV5NJ_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HHY6484862.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACV5NJ_000747"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACV5NJ_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HHY6484863.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACV5NJ_000748"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACV5NJ_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HHY6484864.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="ACV5NJ_000749"
     CDS             23883..25052
                     /locus_tag="ACV5NJ_000749"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HHY6484865.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         7577 a         5089 c         4061 g         8496 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caa
//
LOCUS       DBKOIJ010000023        24765 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000023 DBKOIJ010000000
VERSION     DBKOIJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.023"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            172..900
                     /gene="purC"
                     /locus_tag="ACV5NJ_000750"
     CDS             172..900
                     /gene="purC"
                     /locus_tag="ACV5NJ_000750"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HHY6484866.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            900..1154
                     /gene="purS"
                     /locus_tag="ACV5NJ_000751"
     CDS             900..1154
                     /gene="purS"
                     /locus_tag="ACV5NJ_000751"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HHY6484867.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1156..1827
                     /gene="purQ"
                     /locus_tag="ACV5NJ_000752"
     CDS             1156..1827
                     /gene="purQ"
                     /locus_tag="ACV5NJ_000752"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HHY6484868.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1828..4050
                     /gene="purL"
                     /locus_tag="ACV5NJ_000753"
     CDS             1828..4050
                     /gene="purL"
                     /locus_tag="ACV5NJ_000753"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HHY6484869.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4035..5474
                     /gene="purF"
                     /locus_tag="ACV5NJ_000754"
     CDS             4035..5474
                     /gene="purF"
                     /locus_tag="ACV5NJ_000754"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HHY6484870.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5497..6549
                     /gene="purM"
                     /locus_tag="ACV5NJ_000755"
     CDS             5497..6549
                     /gene="purM"
                     /locus_tag="ACV5NJ_000755"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HHY6484871.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6546..7124
                     /gene="purN"
                     /locus_tag="ACV5NJ_000756"
     CDS             6546..7124
                     /gene="purN"
                     /locus_tag="ACV5NJ_000756"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HHY6484872.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7121..8656
                     /gene="purH"
                     /locus_tag="ACV5NJ_000757"
     CDS             7121..8656
                     /gene="purH"
                     /locus_tag="ACV5NJ_000757"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HHY6484873.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8672..9946
                     /gene="purD"
                     /locus_tag="ACV5NJ_000758"
     CDS             8672..9946
                     /gene="purD"
                     /locus_tag="ACV5NJ_000758"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HHY6484874.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10068..10982
                     /locus_tag="ACV5NJ_000759"
     CDS             10068..10982
                     /locus_tag="ACV5NJ_000759"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHY6484875.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11115..12680)
                     /locus_tag="ACV5NJ_000760"
     CDS             complement(11115..12680)
                     /locus_tag="ACV5NJ_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHY6484876.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12673..14124)
                     /locus_tag="ACV5NJ_000761"
     CDS             complement(12673..14124)
                     /locus_tag="ACV5NJ_000761"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHY6484877.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14138..15154)
                     /locus_tag="ACV5NJ_000762"
     CDS             complement(14138..15154)
                     /locus_tag="ACV5NJ_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6484878.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15374..16462
                     /gene="chvE"
                     /locus_tag="ACV5NJ_000763"
     CDS             15374..16462
                     /gene="chvE"
                     /locus_tag="ACV5NJ_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HHY6484879.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16516..18042
                     /gene="mmsA"
                     /locus_tag="ACV5NJ_000764"
     CDS             16516..18042
                     /gene="mmsA"
                     /locus_tag="ACV5NJ_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HHY6484880.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18044..19249
                     /gene="mmsB"
                     /locus_tag="ACV5NJ_000765"
     CDS             18044..19249
                     /gene="mmsB"
                     /locus_tag="ACV5NJ_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HHY6484881.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19506..20483
                     /gene="guaC"
                     /locus_tag="ACV5NJ_000766"
     CDS             19506..20483
                     /gene="guaC"
                     /locus_tag="ACV5NJ_000766"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HHY6484882.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20747..22171
                     /locus_tag="ACV5NJ_000767"
     CDS             20747..22171
                     /locus_tag="ACV5NJ_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HHY6484883.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22213..22470)
                     /locus_tag="ACV5NJ_000768"
     CDS             complement(22213..22470)
                     /locus_tag="ACV5NJ_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484884.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            22632..23741
                     /gene="pdhA"
                     /locus_tag="ACV5NJ_000769"
     CDS             22632..23741
                     /gene="pdhA"
                     /locus_tag="ACV5NJ_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HHY6484885.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23744..24721
                     /locus_tag="ACV5NJ_000770"
     CDS             23744..24721
                     /locus_tag="ACV5NJ_000770"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HHY6484886.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8022 a         4212 c         5591 g         6940 t
ORIGIN      
        1 gatggtataa gattttcgtt tctgataaaa cacgaacatt aaaagaatct aatgccttaa
       61 atgtacatat ttttgttgat ttataaaaag atgattgcta aaatttaaag tgaaaaaacg
      121 aacaaaaaac gatgtatttg cttttaatgt aaataaaaag gagagacgta catggagaag
      181 aaagaacttt tgtatgaagg aaaagccaaa aaaatatatc ttacttctga tgagaacttg
      241 atttggatag aatatttaga tcaagcaact gcgttgaatg gcgcgaggaa agaccagata
      301 agtggaaaag gagaattgaa taatcaaatc acaagtgcaa ttttccgtta tctgactcaa
      361 agaggaataa aaaatcattt tattgaacaa ttgtctaaaa ccgaacaatt agcaaaaaaa
      421 gtacaaatta tccctctgga agtggttatc cgaaatattg cagcaggaag tttctcaaaa
      481 cgtctaggtg ttcccgaagg taaagaattc atcaaaccaa tcattgaatt ctactataag
      541 gatgaccagt tggatgatcc atttatcaat gaagagcata tctatttctt acaacttgca
      601 aagccggaag aaatcagtga gctgaaagcc caagcgttgg ctattaatga aattttgacc
      661 gaattatttg ctaaggtcgg tttggtttta gtagatttca agctagaatt tggccggtcg
      721 aatggacaaa ttttgcttgc ggatgaagta acaccggata cttgccgatt atgggatgaa
      781 cgtacgaatg aacatatgga caaagacgtt taccgcagag atctaggtga tttgatccca
      841 gtctatcaag aagtatataa ccgtctatcg gaactaactg aagaaaagga gcgagtatga
      901 tgtatttcgt aaaagtatat gtaacctata aagattcagt gttagatcct caaggggagg
      961 cagtaaaaaa agcagtccat cgtatgggat atgaaacaat cgaagatatc cgaataggaa
     1021 aatactttga gatacaactt gctgatgcag aagaaccaat cgatacagtg attgaaacga
     1081 tttgtgacaa gctgcttgca aatgtaaata tggaaacata ccgatatgaa atcaaaaaaa
     1141 tggaggaagt ataacatgaa atttgcggtc atcgtttttc caggttcaaa ttgtgacgca
     1201 gatatgttat gggcagttcg cgagatcatg ggcgctgatg ccgaatttgt acggcatgat
     1261 gagtcctctt tagaaggatt tgatggggtt ttactgccgg gaggattctc ctatggtgac
     1321 tatttgagat gcggtgcgat tgctcggttt tctacaatca tggaagaagt gattcgtttt
     1381 gctgaagaag ggaaaccagt attcggtaca tgcaatggat tccagatatt gacagaagca
     1441 ggacttttgc caggtgtact gagacgaaat gagtctttgc attttatttg caaaactgta
     1501 ccattgaaag tcaaccccaa gacggccttt acttcagagt atgaagccga cgaaataatc
     1561 tatcttccag ttgctcatgg ggaaggaaat tatttctgtg acgaagaaac acttcaagaa
     1621 ctaaaaaaaa ataatcagat tgcctttact tataatgaca acatcaacgg cagcgtggaa
     1681 aacattgcag ggattacgaa caaagcaggg aatgtgttag gaatgatgcc tcatccagaa
     1741 cgtgcagtag aagacttgct aggatcagaa gatgggaaac ggttctttgc ttccatttta
     1801 aaaaacttcg gaaaggtgac cgtatagatg aaaacattga aagaaccgac agctgaagag
     1861 atcaagaaaa aaagaatcta tgccgaatgg ggattgactg atgaagaata tcgaatgatc
     1921 gaagaagata tcttaggaag aatgcctaat tatacggaaa ctggactgtt ttctgttatg
     1981 tggagcgaac attgttccta taaaaattct aaaccggttc tacgcaaatt tcctactagt
     2041 ggacctcatg tgttgcaagg ccctggagaa ggcgcaggca tcgtcgacat cggtgatgga
     2101 ctagcagttg tcttcaaagc agaaagtcac aatcatcctt cagccgtgga gccttatgaa
     2161 ggagctgcga caggagttgg cggaatcatc agagatattt tcagcatggg ggctcgtcca
     2221 attgcgttgc tagattcatt gaggttcggc gaattagata atccaagaac gaaatattta
     2281 ctagaagaag tagttgcagg tatcggcgga tacggcaatt gtatcggcat accgactgta
     2341 ggcggcgaaa ttgcatttga tccttgttat gaaggaaacc cattagtcaa tgcaatgtgc
     2401 gtaggtttga ttgaacacaa acatatccag aaagggcaag ccgctggcgt cggtaattcg
     2461 atcatgtatg taggtgcaaa aacaggtcgc gacggtatcc acggagcaac ttttgcctca
     2521 gaagaattta atcaagaaga agaacagcaa cgttctgctg tacaagtagg cgatcctttt
     2581 atggaaaagc tgctgcttga agcgtgtttg gaattaatct tgcatcattc ggattctcta
     2641 ataggtatac aggatatggg agcagcagga cttgtttcat ctagtgcgga aatggcttca
     2701 aaagctggct caggacttat attgacatta gatgaagtcc cacaaagaga aactgggatg
     2761 acaccttatg aaatgatgtt atcggaatca caagaacgga tgttgatttg tgttaaatca
     2821 ggagaggaag aaaaaatcca agaactattt cagaagtatg atctagatgc agtcactatc
     2881 ggtaaagtaa cagacgacgg gcaatatcgg ctatatcatc aaggagaaga agtagcaaat
     2941 ctgccagtcg atgcattagc tgaagatgca ccagtttatc ataaagaaat gaaagaacca
     3001 gctcgaatcg tagaatttca gcagttagct ccttatcagc cagttatcga agaacctgga
     3061 caagtattgc ttgatctgct gcagcaaccg acgattgctt caaaacgttc aatctacgaa
     3121 acttatgatt cacaagtaca aaccaataca gttgtccgtc caggaagcga tgctgctgtg
     3181 atgagggtta gagggacgaa taaagcgtta gctatgacca cagactgcaa cgcgcgctat
     3241 ctgtatttgg atccaaaaat cggaggacaa atagcagtgg cagaagctgc tagaaatatt
     3301 attgctagcg gtggtaagcc cttggcgatc accgactgcc tgaactatgg ttctcctgat
     3361 aaaccagaag ttttttggga attatcgact tcagctgacg gaattgctgc tgcgtgtgaa
     3421 acgttaggaa ctcctgttat ttcaggaaat gtctctttat ataatgaaac ggatggtcaa
     3481 gcaatctatc caacaccaat gattggtatg gtcggattga ttgaggatca taaacatatc
     3541 actactcagg aattcaaaaa atcaggggat ctaatctata tattaggtaa gacgtttgca
     3601 gattttgatg gcagtgaatt acaaaagatg cagcttggaa gaatcgaagg tgtgatccga
     3661 aactttgatc tatccattga aaagagaaac caagagttag tattgacagc catccaaaac
     3721 ggattgattg aaagtgctca tgactgttca gaaggtggct tggcaattgc actggcagaa
     3781 tcagctttca aacatcagct agggatatct gtacagtttg aattgtcctc tgctcaatta
     3841 tttgcagaaa ctcaatcaag atttgtgttg acagtcgcac cggaaaataa gacacgattc
     3901 gaagaaatga tgggagatgc tgctgtttta gcaggtaagg tgacggatga agcgataatc
     3961 gaaatttcag caactgacgg tcagatcaaa atagaaactg cagtagcaag aaagtgctgg
     4021 gaggatgcaa tcgtatgtct tatgaagtga agagtttaaa tgaagaatgc gggatcttcg
     4081 gtgtttgggg tcatccagac gccgctagtg tggcatactt cgggctccat agtcttcagc
     4141 atcgaggaca agaaggtgca ggtatcgtgt ccaatgatcg aggaaagctt aaggggcatc
     4201 gagatttggg gctgctttct gaggtattca aagatcaaag aaaattgcag cagttaacag
     4261 gagaagctgc aatcggacat gtacgttatg caacagcagg caatggaagt gtggacaata
     4321 ttcagccttt tctattcaaa tttcatgatc agtcgatcgg cttagcgcat aatggtaact
     4381 tgatcaatgc tcgttccctt agaaaagagt tagaggaaaa tggggcgatc ttccattcta
     4441 actccgatac ggagatattg atgcatttga tccgacgaag tgaagaaaca gattttttaa
     4501 ataaattaaa aagtgctttg aatgaagtca aaggtggctt tgcctattta ttaatgacag
     4561 aaacggcaat gattgccgct ttggacccaa atggttttcg tccattagca atcggacaaa
     4621 tgaaaaacgg agcatacgtg attgcctcgg aaacttgtgc attagaagtt attggggcac
     4681 gttttataag agatgtccag cctggtgaga tagtgatcat caatgacgaa ggaatccaga
     4741 ttgattgctt tacgaaggac acgcagcttt ctatttgttc gatggaatat atctattttg
     4801 ccagaccaga ttcaaacatc gcagggatca atgtgcatac tgcacggaaa aatatgggac
     4861 ggaatttagc gaaagaatcg ccagtaaaag cagatatggt cgttggcgta cctaattctt
     4921 ctttgtcagc cgcaagcggt tatgcggaag ctagtggaat cccttatgaa atcggattag
     4981 taaaaaatca atacattgcc cgaacattta tccagccaac gcctgagcta agagagcaag
     5041 gtgttcgaat gaaattatct gctgtacgag gagtagtgga aggtaaaaag gtgattatgg
     5101 tcgatgattc aattgttcgg ggtacgacaa gcagacggat cgtccatctt ttgaaagaag
     5161 caggggcaaa agaagtccat gtgaggattg cttctcctcc tttgaaatat ccatgttttt
     5221 acgggattga tattcaaacg cgtaaagaac tcattgcggc aaatcacacg attgatgaaa
     5281 tcaaagaatg tatcggtgca gattctttag gttttttaag tgaagagggt ctgatcgaat
     5341 cgatcggtct ggaaaaagat gctccgtatt caggattgtg tatggcttat ttcaacggag
     5401 attatccgac gccgttatat gactatgaag aaaaatatca ggaatcacta aaagaaaaag
     5461 tatcgttctt ttaaaagcag accaagaagg gagtatatga attcaatggc aaatgcatac
     5521 gcaaaagctg gcgtagatgt cgaagctgga tatgaagtag tagaacggat caagaagcat
     5581 gtcaaaaaaa cggaacgttt aggtatgatg ggcgctatag gaggattcgg cggttgtttt
     5641 gatttatcgg tctttcaatt aaaagagcca gtccttgtct cggggaccga tggcgtgggg
     5701 acgaaattag cagtggcaat cgaagcagac aaacacgata caatcgggat cgactgcgta
     5761 gcgatgtgtg tcaatgatat cttagcacaa ggcgcagagc cgttatattt cttagactac
     5821 ttagcaacgg ggaaaaatga tccgttacgt ttggaaaatg tagtaaaagg tgtagcagat
     5881 ggatgtgttc aaggaaatat ggcgctgatt ggcggagaaa cagctgaaat gcctggtatg
     5941 tactcaggta atgattatga tctagccgga tttgctgtag ggatagctga aaaatctcaa
     6001 ttattgacag gagaaacgat caacccagga gatgtgttgc ttggaatagc atctagcggg
     6061 attcattcaa atgggtactc tcttgttcgc aaaattttct ttgagcagca gcagttttct
     6121 ttatcatcaa aactgccaga attgaaaaaa acacttggcg aagaattgct tgagccaacg
     6181 aaaatctatg tttcttcctt gcttccgctg atcaaagaaa agaaaataaa aggagctgca
     6241 catatcactg gtggagggtt tgtagaaaat atcccgcgaa tgttaccaga atctatggct
     6301 gcacaaatca aacttggctc atggccgatt cttccaatat tcggctgttt acaaaaatat
     6361 ggtgaaattc ctgagatgga gatgtatgaa atcttcaata tgggcatcgg tatggtcctg
     6421 gcagtccaag aatcgaaagt aaaagaaata gtacacgaaa ttgagcaagc tggcgaaaaa
     6481 gtttatgaaa tcgggaaagt gatcgccaaa gaaaaacatc gtgtggtgtt tataggagaa
     6541 gaacgatgag aatcgctgtt tttgcttctg gaaacggaag caactttcaa gcgctagccg
     6601 attatctatc gaaaaaagga ttggaatctt cgattgactg gttgttttgt gatcagcctg
     6661 aagcatatgt tttgaaacgt gcaactgcgt tgagtgtacc ggcagattgt ttttcaccta
     6721 aagaatttga ttcaaaaaaa gagtatgaag aagcaatttt gcataaattg aaagaaaaaa
     6781 agatcgattt gatcgtatta gctggatata tgcgaatcat tggaccggta ttgctgaaaa
     6841 attatgataa aagaatcatc aatatccatc cgtctctttt acctgccttc cctggtttgc
     6901 atggcatacg tgatgccttt gaagctggag taaaagaaac gggtgtcaca attcattata
     6961 tcgaccaagg tgtagatact ggaccaatca tccggcagga aaaagtgaga attgaacagg
     7021 aggacacgtt cgattcttta gaagaaaaaa tccatagagt agagcatcga atctatccag
     7081 aagtgattag tgaaattatt gaaaatggag gctatctgaa atgacaagag cattgatcag
     7141 tgtatcagat aaaactggga ttgtagcatt cgcccaaggt ttgattgaac aaggagtaga
     7201 aatcatttca acaggaggaa caaagcatgc gttggataaa gctgggatca aaacgctttc
     7261 gatcgaagaa gtgacagggt ttcctgaaat gatggatggc cgtgtcaaaa cacttcatcc
     7321 taaaatccat ggtggtcttt taggaagacg cgatctccct agccatatgg aggcaatgga
     7381 aacggaaaat atccaaccaa tcgactttgt ttgtgtcaat ttatatcctt tcaaagaaac
     7441 gattatgaaa ccagatgtcg aaatagcaga agctattgaa aacattgata tcggtggtcc
     7501 tagtatgctc cgtagtgcag caaaaaacca tgaatttgtc acctctgttg ttgatccatc
     7561 tgactatgaa gaagtacttt cagaattacg tcagtatggg catacttcac tttctttcag
     7621 acaaaaacta gcggcaaaag tctttcgtca tactgcggca tatgatgcac tgatcgctca
     7681 atacttaacg gattgggtgg aagaaaaaga accggaaaaa ttgacactta cgtatgaacg
     7741 aaaacaaacg cttcgttacg gtgaaaatag ccatcaagcc gctgcctttt atcagtccgc
     7801 tcttctggaa aaatattcga tcgcagcagc aaaacaactc catgggaaag aactttctta
     7861 taataatatc cgagatgcag atgctgcgct acggatcatg aaggaatatg acgagccaac
     7921 ggtagtcgcc ttgaaacaca tgaatccttg tggtattggg acagcatcta cgattttaga
     7981 agcgtgggaa gcagcgtatg aagcagatcc tgtttcaatc tttggtggga tcatcgtttt
     8041 gaatcgagaa gtagatctgc caacagcaga acagatgcat aagctgtttc tagaaatcat
     8101 cattgcccct tcttatacac aagaagcttt ggaattactg caaaaaaaga aaaacatccg
     8161 attactgaca gtagactttg cgcaggaaga aaatcaaaat cgagaagaaa cggtctcagt
     8221 cttaggtgga cttcttgtac aagatcaaga tctggcaata gaaaatgaag aagagtggaa
     8281 agtcgtgaca aaacgtaagc cgactccagc agaagcaaaa gcattagcgt ttgcttggag
     8341 agctgtcaaa catgtgaaat ctaatgcgat cgtactggca aacgaacgtc agacagtagg
     8401 aatcggagcc gggcagatga atcgagtcgg ttcagtaaaa attgcgattg aacaagcgca
     8461 agagaaaatg gaaggtgcag ttttagctag cgatgcttat tttccaatgg atgattcggt
     8521 tgaatatgca gccaaacatg gtataaaagc aatcattcag ccaggaggaa gcatcaaaga
     8581 ccaagattct attgatatgg cgaataaata cgatctagca atggtcttta ctgatgttcg
     8641 gcattttaga cattagagag gagtcggata aatgaacatt ttggtgatcg gtgcaggtgg
     8701 tcgtgaacat gcaataagta aaaaactttt agccagtccg aaggtggata cggtgtattg
     8761 tgcgccagga aatccgggga tgacaaaaga tggtatcaat tgtgtcgcta tcagtgagcg
     8821 agatcattca gcgttaatcc attttgttaa gaaagaggct attgattgga gtatcgttgg
     8881 tcctgaagtt cctttgttaa atgggatcgt cgatgatttt atggcggaag gattgaaaat
     8941 cttcggtccg acaaaagcag cagctctgat cgaaggttcg aaaagttttg ctaaacaatt
     9001 aatggatact tatcaaatcc cgacagctaa atccagaagt tttgaatgtt acgaaaaagc
     9061 agcagcgtat atcaaagaaa atggtgcgcc aatcgtagtg aaagcggacg gcttagcagc
     9121 tgggaaaggt gtagtcgtgg ctaccacagt gaaggaagcg ctggatgcgg cagaacaaat
     9181 gttgaaccag catcgatttg gggatagtag caaaaaaatc gtcgtagaag agtttttggc
     9241 aggagaagaa ttctcccttt tggcgtttgt ccgtaatacc aaggtgtatc cgatggtcat
     9301 ttcgcaagat cataagcggg catttgataa cgataaaggg ccaaatacag gcggaatggg
     9361 tgcttattca ccagtaccgc aaatttcaaa aaagatgatt gaaaaagctg tggaagaagt
     9421 tttaaaacca gcggctttgg gaatgaaaga actaggacgc tctttcacgg gtgttttata
     9481 tgcgggtttg attgctacga aggaaggacc aaaagtgatc gaattcaatg cgcgtttcgg
     9541 tgatcctgaa acgcaagtcg ttctttctcg gttgaagagt gactttgcgc aagtaatcga
     9601 tgatttatta gagaatcgta cagtagagtt aaagtggcag caagaaggat acaatgtagg
     9661 cgttgtggtt gcagctgccg gttaccccga agaatatgag acaggaatgg ttctaccaga
     9721 ttttctggaa gaagagcttt ctgtttacta tgctggagtt tcctcaaata acggcaaact
     9781 agttggagca ggaggacgtc tttatttagt agaagcatac ggtgagacga tcaaagaagc
     9841 acagaaaaaa atttacaccg cgttaatgga taaaacgaca gatggaacat tttatcgtac
     9901 agatattgga gaaaaagctg ccaatagcga actgatcgtg aagtaaatca gctgcttcca
     9961 tttataaaaa atacaacaaa cataatcgga agcgccgatt ttagtgaagc cttttcataa
    10021 agagtgtggt acacttttgt tgtgtatata aataaggagg agttcagatg aaaaaaattc
    10081 aattaggtac ttctgatcag aaagcagctg cagtgatctt aggctgtatg cgtatcaaca
    10141 atgcggaaga tccagtaaaa gtgattgaga cagcttatga aaacggcatt gatttttttg
    10201 accatgcaga tatttatgga aacggtgaat gtgaagaaat ctttgcagat gctctagcaa
    10261 aaacatcaat caaacgagaa gatcttttta ttcaaacaaa atgtggaatc gttccaggtg
    10321 tgatgtttga tttttctaaa aaacatatca ttgaatctgt agaaaaaagt ttgacccgtc
    10381 tgaaaatgga ttatgtggat gctttacttc ttcatcgtcc agatacattg gtagaaccag
    10441 aggaagtagc tgaagcgttt gatgaattgg aacggtcagg aaaagttcgt tattttggcg
    10501 tcagcaatca aaaaccaatg caaatcgaat tgctgaaaaa atatgttcgt caaccattag
    10561 tagctaatca gctgcaattt ggcttgaaac atactggaat gatcgatcaa gggattcatg
    10621 tcaatatgac agatgaagca agtatcgatc atgacggtag tgtacttgat tattcccgat
    10681 tgcatgatat gacgatccaa gcatggtcac cataccagta tggatttttt gaaggtgtat
    10741 ttatcgggaa cgaaaaattc cctgaattaa accaaacatt agaagaacta gcagctaaat
    10801 atcaaacgac accaactggc ttggcttctg cttggatttt acgccatcca gccaatatgc
    10861 aagtgatcgc cggtacgatg aacgtagggc gaatcaagga aatcgctcaa gcttcagaaa
    10921 ttaatatgtc tcgggaagat tggtacgaat tgtaccgtgc tgctggaaac attttacctt
    10981 aaagattgtc ataagcaggt caaaagggct ataaattaaa aagacagaat aacttgaaag
    11041 ctgttttcag cttcgagata ttctgtcttt ttttattgca aatggaaaga gtatcattgt
    11101 acggatttcg tttattagtt atttttgttt ttaaggaaaa gctgtgtccg atattctttt
    11161 ggagaaatcc cttgtttttt cttaaataaa taactgaaat agttagggtc attataacca
    11221 atttcaaaag caatatctga aatacgatag tctgattgct gcaataattt ttttgctaat
    11281 tcgattcttt gttctgtcag ataatcgatg aatgtttggt taaacgattg agaaaaaatc
    11341 gtactgaaat gagagggact taatgcaact tgttgagcga cccaagttaa ggacatatct
    11401 ggatcggtaa aattttcttt gataaaatcc aatgcatggt tcaaaattgg ctgatatttg
    11461 ttccttgaag gctgtgaggg cagagcagtt aagaaagaaa tgaacatata aactgtcgaa
    11521 cggtaaagcg agtcgtcggc gctgattgct aagcgttgac tggtggatgt gcaatgaacc
    11581 atttcttgcg ggattttctt ttctttcagg cagacatgct ggagtagctt agtcaagaca
    11641 aataaacgat ataatcgtgt tcgagttggt gtgccttgtg gtttcgtcaa gtgttcgatg
    11701 tacttagata cttcgttttt cgataatgaa gacaaatcaa gtgagaggtc aaagtaaaag
    11761 acagaatcaa ctggaagttt cgcttcttga atcaaatcct catagctgat aatttgttct
    11821 ttaagggaac tggcggtttc aagtatcatc tgcgtgatct caaaagatgc aggtaattcg
    11881 ctgattcttt ctgcgacagg accaatggcg acagaaaaat tatttttttg tcccttttct
    11941 aattcatgga ccaacgtttg ggctacctga taactggtct cgatcacatt ttcttcaaga
    12001 gaatcaacta ttaaaaattt gatgaatcga gaggaaacag cagagaagat cacttgttga
    12061 ttctccctaa ataaaaactt caaataatca ctgagatgag cgtaatcttc aaagtctgta
    12121 ctgacttggt ttgctgctaa taatacggtc atttttttcc ctgctaaaga gcgattgaaa
    12181 gattcagcaa gtttcaatgt ttcactgatc gtaagtgagc ctttgaacaa accgttcaag
    12241 aaatgatttt ttttcaattc gaaaacaaga tcagaagaag tgtgcgtttc tttttgctgc
    12301 aaatctaaag cagttactgc tttttctatt gcttgtttca attcgctact tttaatcggt
    12361 ttaagcagat aatcatctgc ctgtacctgg attgctcctt tcatataatc gaaatcgtcg
    12421 aatccactaa taaatatgag acgagtccaa ggaagtagtt tccttgcttc ttttgaaaaa
    12481 gtcaacccgt ccatgaaagg catttggata tcagtcagaa tgatgtcggg ctgtaagtcg
    12541 ataattgaag acagtgccat ctcaccatct ccagcttcgc cgacataagt gattggataa
    12601 taagaaccaa ggacagttat ttgttcccgc aagttattgc gaatgaggta ttcgtcttcg
    12661 atgataaaaa ctttatacat gtttgtttcc tccaattgtt ttgggagata gagggatcat
    12721 cagtgtgaca gttgttcctt ctcggtattt gctgtcaatc gtgataggtt tttgatcatt
    12781 tccgtaataa agaaggagac gcttgtttac gttgtaaagt ccgtagccga tactgaaatc
    12841 tgaatcgatc cctttggcta actctttacg tatttctgcc agtttttctg cagggatgcc
    12901 aatcccgttg tctataactt tgaattggat catctcatca attttgcatg tggtaatacg
    12961 aataaatcct ttgtgacgga cgtacttcgt tccatggtat acagcatttt caatcaatgg
    13021 ctgcaagatc atttttaaaa tagaaacagg aaaaagtgtt tctggtatat cgatcttata
    13081 gtcaagcata tggccgtatc ttatttttaa gatgactaga taatcattga tatggctaat
    13141 ttctttttca agaggaatcc agtcttgtcc tttgcttaaa gacgtacgaa aaaagccgga
    13201 aagtgcatgg gtcatagtca gagcttcttc aaagcagtct tgttcgatca aagaaataat
    13261 ggcatccaaa ctgttataaa taaaatgggg agtaatctga gcttgcaata cacgaacttc
    13321 actttgtgct aaatggtatt gtttcaaagc gttctcccgt aaagactggt ttagatcttc
    13381 cgccatttta ttcatgctag cggtaagtgt ttgtaattcc agtgtttccg gcagggttaa
    13441 acgaaagcca aggttccctg aagaaagttg ttctgacatc tcgatcagct gattgatcgg
    13501 ttcttggacc tttctctcta aaaagcgacg attctttttc gcatagaaaa aaatggtact
    13561 aagaactcca agttccacta ttgttaatag aatgagcgag cggacaagtg tcgtattttt
    13621 tttgcttgct aagtcgatct ccacacgaac aaaatcttgt aagatatctg acagcagttg
    13681 agtgacagaa tctacctgat tgatgatctc ttcattggta tccataaaat cttttgccaa
    13741 gatattattg atcagctcgt tttgataatc ttccagtgta tcgatcgtac gtagcgatac
    13801 atctaacgta ctaatttctt taagtgtatt tgtattattt ttgatctctc gaatatcatt
    13861 cctaagttct tctaggatat tttcctcatt gaactcatct acggttactt ggccagaaat
    13921 caagtcccac atactttcca aaactttttg atcaaccttc aaagcaattg catctgcttt
    13981 ttggatattt gcgatcgtct gttgataaat caaaagctgt tgcgaatata acagtatgct
    14041 cagtagcaaa gggataacag caaatagaag catggctttg tttgcttggg caaaaatctg
    14101 ttttatgctt aaattttttg tcatgatact tctccagtca ataatttctg gttgggatgt
    14161 tagctatatt ctgctgggaa aagacagtct cggaaataaa aatatcttta ggtaatgttt
    14221 ttcctgtttg aatatttcca aaataacgag taacggtatt ggctacatac cagccgacat
    14281 caggtttaca ctcgactaca caattgacga ttcctgagcg tagcttctcg atcatatctt
    14341 cttgtccatc aatagttaca atgatcaaat cttctccagg tttgattgat gtttctttta
    14401 tcgcagctaa agcaccgtga gtcatttcat cactatggct gtataataca tcgatttcgg
    14461 ataaatcatt tgtttctaaa tatcgtttca tctggtcttt tcctttcatc cgaataaaat
    14521 caccgacaat tcggtgaaca atagtcatcg gaggtttatg gtcgatggtt ccatcttttt
    14581 ttagggttgg tttacgattg attgattcca aaaatccttc ggagcgtagc ttggtcggtg
    14641 tggagttctc tgaaccagca agttctagga ttctgatttc ctgtttcttt gaatttgtga
    14701 agtgattgct gacaaataaa cccgcccgat ttccttctgc tttgaaactg gggccaatgt
    14761 gggtgacgaa aagctcggga tcattcgttt gaatatgtct gtcgacaagt atgacaggga
    14821 tccctgctcg ctttgcttct tttaatacag agtcccaccc ggattcttgc aaaggagtaa
    14881 aaatgatcat atccacttta taggcaatga acgtccgtat atcttggatt tgccgttcct
    14941 gattcatgta gccatttcgg tagagaactt ggtaatcttc tttttcaagc gcagtgataa
    15001 ttgattcagt atgctttttc cgccagctac tctccgttcc tgattgcgaa aaaccaacga
    15061 ctagtgggtc aggtgcttcc tttgtattag gtgtacaccc catcatttgg taagcgatta
    15121 caacaagggc taaaaaaaat aaaacacgtt tcactttaaa tcactccatc tggatttgtt
    15181 agtactatct tatcagataa aagggaaaat caaagtcaaa aggaatcaat cgaaaaaaat
    15241 ccaagaaact taaaaaaatc caagacaaaa aaagtacacc taaaaaattt aaagtaaatc
    15301 ccataaaaaa atcaatgtta acgctttctt tattagctat actcggtgta tcaattgaaa
    15361 tgggggataa aaaatgaaaa acaaatttag aaagattgtt gggactatcg ctattttgac
    15421 cgcaagcatt ctattaagtg cgtgtggcaa ttcaggatct gcagatgata gtacggggta
    15481 tgtaggaatc gctatgccga ccaaatcagc agaaagatgg atcgctgatg ggaacaacat
    15541 ggtctcggaa ttagaaaaac tgggttataa aacagatctt caatatggag aagataaagt
    15601 agaaaatcaa gtagcacaaa tagaaaatat gattacaaaa ggggtagata cacttgtgat
    15661 tgcttctatc gatggttcgg ctttaactga tgtgttagct aaagcaaaag aagcagatat
    15721 caaggtgatc gcctatgatc gtttactgat gaattcagaa aatgtcgatt attacgcgac
    15781 ttttgataat tttggagtag gtgtatcgca agcagcttat attgaagagc acttaggatt
    15841 gaaagaagga aaaggaccat tcacgattga attatttggt ggctcgcctg acgacaacaa
    15901 cgcactaatc aattacaacg gagtgatgtc tgttttacaa ccatatatgg acaacggtca
    15961 gttggtcgtg ccatctggac agacgagttt cagtcaaatc gctacgcttc gctgggatgg
    16021 ttctacggcg caagcaagga tggataatct gttaagcgca aattatactg accaaacatt
    16081 ggatgctgtt ttgtcgccat atgatccaat aagtttagga atcatttcgt cattaaaagg
    16141 cgtggggtat gggagtgaaa gtaaaccttt acctgtgatt actggacaag atgcgacagt
    16201 tgctggcgtt aaatcgatca ttgccgggga acaaacacaa acgatcttta aagacacacg
    16261 tatcttagca aaaaatacga ttgaaatgat caaagcaatc agtgatggag aagaggttcc
    16321 ggtcaatgat actgaaactt atgataatgg ggtgaaaact gtccctacgt atttagcaaa
    16381 tacagtatct gtcgataaag ataattatca agcagagctg atcgacaccg attattataa
    16441 agaatccgat ctgaaaaatt aagaggaacc aaacaaggaa ctaaagagat ctaagtcaga
    16501 gagaggataa gaattatggc ggattgtatt ttaaaaatgg cagatatcac caaaaaattt
    16561 tctggaatcc gagcgttaga ttgcgtgaat ctgatgattg aacgtgggga aatccatgca
    16621 ttgtgcggag aaaacggagc gggaaaatca acattgatga atgtcttgag cggcttgtat
    16681 ccatatggct cctatgacgg ggaaatcttt tatgatggaa agctgtgtca atttaaagat
    16741 ttgaaagata gtgaagaaaa aggcatcgtc atcattcatc aagaattagc actaagtcct
    16801 tatctttcaa taaaagagaa tatttttctc ggaaatgaac aagcagaaag aggcgtgatt
    16861 gattgggaac tgacagaaaa gaaaacatat gaactgctga ataaagtcgg tttgtctatc
    16921 gacccgaatc ttctagtatc ccagattggc gtagggcaac agcaactagt tgaaatagct
    16981 aaagcattat caaaatccgt ccgtctgttg attttggatg agcctaccgc tgcattgaat
    17041 gaagaagaaa gcgcgaattt gctgagattg atccgagagt tgaaagagca aggtgtaacc
    17101 tccatcatta tttcacataa gctgaatgaa atcgtggata tcgcagatcg aatcaccatt
    17161 ttacgggatg gacagacgat tgagacacta gaaaatgaaa tgatcagcga agagcatatt
    17221 attcgcggaa tggtgggcag agatttagtt gatcgttatc ctgaaagaaa accggatatt
    17281 ggagaagttt attttaaagt agaagactgg acggtctatc accctcttga tcacgagcga
    17341 atcataaacg accacatcaa cttgcatttg aaaaaaggag agattgtagg aatagctgga
    17401 ctgatgggag cgggcagaac tgaattcgct atgagtctat ttggacattc ttatggaagc
    17461 aagatatccg gacgtatatt aaaggaaggg gcagagatat cagttaagac cgttccttca
    17521 gccattgcaa acggactcgc ttatgtctcg gaggaccgaa aatctttagg actgaatctg
    17581 ctgatggata ttcgggaaaa cgtgacgctg agcagtttgg ataaaataag tcaaaaagga
    17641 gtattagata aagaaaaaga agtaactgag gcagaaaatt tccggaaaaa gatgcgtata
    17701 aaaacaaact ccatccatca aattgtcagt agtcttagtg gtgggaatca acaaaaagtc
    17761 gttttatcca agtggctgat gacacaaccc gatattcttt ttttggatga gccaacccgt
    17821 gggatagatg ttggggcaaa atatgagatc tacacgatta tcgaagaaat ggcagcaaat
    17881 ggcaaagctg tctgtcttat ctcttctgaa cttcctgaaa tattagggat gtgtgatcga
    17941 atctatacga tgaacgaagg acgtattaca ggagaagtct cgcgagaaga tgcagatcag
    18001 gaagtcttga tgaaattaat gacgaaagaa gaggtgggtt aagttggaac aggcaaaaaa
    18061 tcttacggat aaaaaagaga aaaaactaaa tttgaaaagt agagcagctg atctttttgg
    18121 tcgctacagc atggtgatca ttttagcggg actacttatt gcgtttcaat tgatgacgga
    18181 tggtattttc tggcgtccat tgaatataac taaccttgtt ctgcaaaata gccatatatt
    18241 agtactagct gcagggatgt tattagttgt gctcttaggt catgtggatt tgtcggtagg
    18301 gtcagtgatg gcgtttgttg gtgcaattgc gggcatgatg atggtcaatc gccaaatgaa
    18361 cccgtggatg acagttatcg catgtcttgc agtaggcggg ctgatcggcg catggcaagg
    18421 tttttgggta gcttatgcag ggataccggc gtttatcgtg actttagcag gattgctgat
    18481 gtttcgtgga ctgacacaag ttgttctagg aggccaatct ctagcacctt ttccagctgt
    18541 tttccaaaaa atgtctacgg gttatttacc agacttaagc gaaggaagca gccttcactt
    18601 cttgacattg attttaggaa tgatcatcac gatcgtgtta gttgttggtc aatggcgaac
    18661 aagaaatcgt cggaaaaatc atttatttga agtggaatct ccaacagctt tcttggtaaa
    18721 ggcggtattt acagcagtcg ttgtactagg aataacttat atctttgcct cttatcaagg
    18781 gtatccgatt attttgatca ttcttggttt gatcgttgca gcatatgggt ttttgacaaa
    18841 taaaacagta gcaggtcgac agatttatgc gacgggcgga aatcgaaaag cggctgaact
    18901 atcagggatc aaaacaaagc gaatcacgtt ctgggtattc gtcaacatgg gagtcatggc
    18961 agctttagct ggattagttt tagctgctcg attgaatgcg gcaacacctc aagcaggtac
    19021 atcgatggaa ctggatgcaa tggcggcagt atattttggt ggtgcttcga cttccggcgg
    19081 cattggaaca atcatgggtg cgattgtcgg tggcttggtc atgggtgtat tgaacaatgg
    19141 catgtcgatt ttaggcgtcg gggtagactg gcagcaggca atcaaaggat tgatcttgct
    19201 tttagccgtt gtgctagact tgtataataa gaaaaaacga acaacgtagg agaacgccgg
    19261 ttattcagaa aaaagactgt gacaagacat ttgtcacagt ctttttttcg ctaaaagcga
    19321 acattcattg tttcgtttgt atagactaaa aataatatat gtttttttac cattcgtata
    19381 aattctcttt ttgctcttga tttttttgct taattgagtt aaattacata gggcgctaaa
    19441 aaaagccttg attaaattaa taatgttcgt gttttgctgg atgtgaacat caggaggata
    19501 atgtaatgaa agtatttgat tacgaagata ttcaattaat tcctaataaa tgtatcgtaa
    19561 atagccgttc tgaatgtgat acaacggtca cattaggtaa acatacattc aaaatgccag
    19621 tagtgcctgc caacatgcag acgattattg atgaaacaat cgcagaattc ttagcggaaa
    19681 atggttattt ctatatcatg catcggtttg atgaagcagc tcgaattccg tttatcaaaa
    19741 aaatgaaaaa gcgtggattg attacctcga tcagtgttgg cgtcaagaaa gaggaatatt
    19801 cctttataga gaagttggca gaagaatcgc taaatcctga ttatatcact atcgacatcg
    19861 cgcatggaca tgctaactca gttattgata tgattcagca tattaaaaaa tatttgcctg
    19921 aaacttttgt gattgccggt aatgttggta caccggaagc cgtccgtgaa ttggaaaatg
    19981 cgggcgcaga tgcaacaaaa gtcgggattg gtccaggaaa agtatgtatt acaaaaatca
    20041 agacaggctt tgggactgga ggctggcaac tggcagctct tagatggtgt gcgaaagcag
    20101 ctcgcaagcc gatcatcgct gacggtggga tcagaacaca tggcgatatt gcaaaatctg
    20161 tccgttttgg tgcgacaatg gtcatgattg gttcattgtt tgccggtcac gaggaatctc
    20221 ctggcgaaac aaaagtcgaa aatggaatcg tgtataaaga atactttggt tctgcttcag
    20281 aattccaaaa aggcgaaaaa cgaaatgttg aaggaaagaa aatctggatc caacacaaag
    20341 gatctttgaa agatacacta gtggaaatgc aacaagacct tcaatcatct atttcttatg
    20401 ctggaggaag agatctggaa gccattcgaa aagttgacta tgtcatcgtg aaaaattcga
    20461 tttttaatgg agacacaatc taaaaaataa ataaaatatt aggatgttgt gaaaaagttg
    20521 tcctgcatta agtaataaga acagccaaac aaaaatagtt ctttatatgt tttgtttggc
    20581 tggaattatt atcgcagata caagcttttg aacatcatta ttcagaataa gggactgtga
    20641 taagattttt gtcacagtcc cttatgtttt gcaaaaaaag tttttctttt ttctcgtaaa
    20701 ttcggtaaaa tcaaaagaaa aaatagacaa tagaaaagag aagcttatgt gggaaaaagt
    20761 agagaaaaaa caaggaccga tttatcgtca aattatggaa cagattatga gagaaatcga
    20821 aaacggaagg atcaatcctg gaagtcaact tgaatcagaa cgaaaactag ggcaactttt
    20881 tggtgtgaat cgttccaccg ttgtccatgc attggaagag cttagggagc ttggggtact
    20941 aacgagcaaa cgtggcagcg gatggtttgt taatcagact gaatggggga aattcgctgt
    21001 tccaagaatc gattggcggc aaatgatctc tcctcgttat gagcagacgg actggtatga
    21061 gcaaaaaata agagaagcaa agcagatcca aaaaacatcg tttcttgatt tatacgctag
    21121 cgagatgcca gatgagcttt tgccgaattt tgagtttcct tcttattctc ttgaagaaat
    21181 cttagcggaa gaaaaagaaa tggatctttt aggctatact ccgttacgta acaaaatcaa
    21241 acggtatttg gaaagtaaat ttgaattggc attttccccc aaccagcttc ttgtgactgg
    21301 tggcggtcag caagcaatct ttttgatttt acaaacgatt ctttcagctg gagaggggat
    21361 agctgtagag tcaccttctt ttttttaccg attacctctt ttcaaagctt caggaacgcg
    21421 tttatttggg ataccgatgg atgaagaagg gatcgactta gaaaaactga aagaatcgat
    21481 ccataaaaat aaattgaaag cagtgcttat caacccgaat tttcaaaacc ctacaggaaa
    21541 agtgatgagt caaaaacgac gagaaaagct agtagaattg tgtcgacagt atcggcttcc
    21601 aattattgaa gacgatgtat ttacggatct ttcatttcta aaccaagaag aagtccttcc
    21661 tatacgaacg attgatccgg ataatgtgtt atacgtcggt tcgttatcta gggttttagg
    21721 aaaaacaacg aaaatcggtt gggtgatagg acctgagact tttatctcgc aactagctca
    21781 agcgcaagaa atgatggagt tttcgatgaa tatccttaca caaatcacag tggctaatgt
    21841 atttaatgaa gcaattgatt tgaaaatgca agatgtaaga aaaaagttga aagaaaaaag
    21901 ccgactgata agcgcttggg gagattcaca atcgtttttc aagcttcatt caattttagg
    21961 tggttactat gcctggatta cttgggatgg gaaaaaacta gaccccgaac tagcagaaaa
    22021 agtagtagat atgggtcttg gaattgcacc aagtctactt tttgggcaag atataaatgg
    22081 aatcagaatc aattacagta ggctggatga aaaccaaata ccgttgtttt attcaaagat
    22141 gaaaattcta gaaaagtggt taacagaata atttcacaaa gtaaatagat taaagaatat
    22201 aacagcttgg atttaaattt caagctgttt cttttttttc ggaaaaagaa tacgatataa
    22261 agaaactaag aaaaaaccat tatttgtacg tccgttcacg atgtgtaaat aaaataaatg
    22321 tttgcaacaa aactgtataa caccaacgtt tttaaataga aacagatggt tgtaagtttg
    22381 attagaaaat agaaaaaatg cagatgaaat ataaaacgct ttccttattg tgattttatt
    22441 cactaatgct gttaatatgc ggattggcat aatttccttt catcgtgaaa atttgtgctt
    22501 ttagcttgta tttctcataa atccatgata atttattaat gaagacggtt aaatagtgat
    22561 acagttttat tcactgtttc aatcgcagaa tgaagagatt ttcttataga tgagagagga
    22621 atgtgtgaca aatggcaaac aaaaaagcac cgatcgactt ccaagcactg ctagatgaag
    22681 tcaatgcaga tttcccagta tatcaagcac ttgatcaaga tggaaacgta gttaacaaag
    22741 atttagtacc tgatttatcc gatgatgaac tcgttgaatt aatgactcat atggtatggt
    22801 ctcgtgtatt agaccaacgt tcaacagcgc tgaaccgcca aggacgccta ggcttttttg
    22861 caccaactgc tggacaagag gcaagtcagc tggctagtgc ttacgcattt gataaagaag
    22921 atgttcttct tccaggttat cgggatgtac ctcaactagt aaaacatggt ttacctttat
    22981 ctcaagcttt tttatggtct cgaggacatg ctgcaggtaa ttcttatccg gaagaattga
    23041 aagctctacc accacaaatt atcatcggtg cacaatatgt acaagcagcc ggtgttgcat
    23101 taggtttgaa gaaacgaaac aagaaaaatg tcgtcttcac ttatactggt gatggcggtt
    23161 catcacaagg tgatttttac gaagcaatca atttcgctgg cgcttaccat gcaaatgccg
    23221 tattttatat ccaaaacaac gggtatgcga tctctacacc tcgtgaaaaa caaactgcag
    23281 caaaaacact tgcacaaaaa gctgtcgcag ctgggattcc tggtattcaa gtcgatggaa
    23341 tggatccgct tgcagtatat actgtatcta aaatggcacg ggaatgggcg atcagtggaa
    23401 atggaccagt cctaatcgaa acactgactt atcgttatgg accacataca ctatctggtg
    23461 acgatccaac ccgctaccgt acaaaagaca cagatgacga ttggcagaaa aaagatccat
    23521 tgatccgctt ccgtaaatat ttgacggaaa aaggactttg gtctgaagaa aaagaagaac
    23581 aagtcatcga agcaactaaa gaagaaatca aagcagctat tgctgaagcc gacaaagtac
    23641 caaaacaaaa ggtttcagat ttcttgaaaa acatgttcga agtatctcca caatcaatca
    23701 aagaacaaat tgcaatttat gaagcgaagg agtcgaaata atcatggcac aaaaaacaat
    23761 gatccaagca atcacagatg ccttagcact tgaactagaa aatgacgaga atgtcgttgt
    23821 ttttggtgaa gatgttggga aaaacggagg agttttccgg gcaactgaag gattgcagga
    23881 aaagttcgga gaagaccgag tattcgatac tcctttagct gaatctggaa tcgcaggatt
    23941 gagtttcggt cttgcgttag aaggatttcg tccagttcct gaaatccaat tctttggttt
    24001 tattttcgaa gcaatggacg aagtagtagc tcaaatggct cgtacaagat atcgaatgag
    24061 cggcactaga aatctaccca tcacaattcg ttcgccattt ggtggcggtg ttcatacacc
    24121 agaattgcat tcggataatt tagaaggttt gatcgctcag tctcctggta ttcgagtagt
    24181 gattccttcc aacccttatg atgcaaaagg actattgatt tcttctatta gaagtaacga
    24241 tccggttgtt ttcctagaac acatgaaact atatcgctca ttccgagaag aagtgccgga
    24301 tgaagcttac gaagttcctt tggataaagc agcagttact cgtgagggaa cagatgtatc
    24361 tattatcaca tacggagcaa tggtgcgtga agcaatcaaa gcagcagaca atcttgcaaa
    24421 agaaaatata tctgttgaaa tcattgatct tcgtacagtt gcgccattag atgtagaaac
    24481 gatcattcaa tccgtggaaa aaactggtcg cgtagttgtt gtccaagaag cacaacgaca
    24541 agcgggcgtt gccgcgcaag tagtttctga gatctcagaa cgcgctatcc tttcattaga
    24601 agccccaatc ggacgagttt ctgcacctga tacagtcttc ccatttggac aagcagaaaa
    24661 tgtctggttg ccaaatgcaa aagatatcga agacaaagta aaagaaatcg cggaatttta
    24721 agaaaagaag ggacgaaaca aagtcatggc ttatcaattt aaatt
//
LOCUS       DBKOIJ010000024        24359 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000024 DBKOIJ010000000
VERSION     DBKOIJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.024"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..832)
                     /locus_tag="ACV5NJ_000771"
     CDS             complement(<1..832)
                     /locus_tag="ACV5NJ_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HHY6484887.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     Y"
     gene            complement(1056..2996)
                     /gene="thrS"
                     /locus_tag="ACV5NJ_000772"
     CDS             complement(1056..2996)
                     /gene="thrS"
                     /locus_tag="ACV5NJ_000772"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HHY6484888.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3333..3512)
                     /locus_tag="ACV5NJ_000773"
     CDS             complement(3333..3512)
                     /locus_tag="ACV5NJ_000773"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HHY6484889.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3559..5382)
                     /locus_tag="ACV5NJ_000774"
     CDS             complement(3559..5382)
                     /locus_tag="ACV5NJ_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HHY6484890.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5660..7939
                     /gene="helD"
                     /locus_tag="ACV5NJ_000775"
     CDS             5660..7939
                     /gene="helD"
                     /locus_tag="ACV5NJ_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HHY6484891.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8032..8679)
                     /locus_tag="ACV5NJ_000776"
     CDS             complement(8032..8679)
                     /locus_tag="ACV5NJ_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484892.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8858..9577)
                     /locus_tag="ACV5NJ_000777"
     CDS             complement(8858..9577)
                     /locus_tag="ACV5NJ_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6484893.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9708..10859)
                     /locus_tag="ACV5NJ_000778"
     CDS             complement(9708..10859)
                     /locus_tag="ACV5NJ_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HHY6484894.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11076..11816)
                     /locus_tag="ACV5NJ_000779"
     CDS             complement(11076..11816)
                     /locus_tag="ACV5NJ_000779"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HHY6484895.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11820..12143)
                     /gene="rsfS"
                     /locus_tag="ACV5NJ_000780"
     CDS             complement(11820..12143)
                     /gene="rsfS"
                     /locus_tag="ACV5NJ_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HHY6484896.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12200..12793)
                     /gene="yqeK"
                     /locus_tag="ACV5NJ_000781"
     CDS             complement(12200..12793)
                     /gene="yqeK"
                     /locus_tag="ACV5NJ_000781"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HHY6484897.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12777..13427)
                     /locus_tag="ACV5NJ_000782"
     CDS             complement(12777..13427)
                     /locus_tag="ACV5NJ_000782"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HHY6484898.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13437..13748)
                     /gene="yhbY"
                     /locus_tag="ACV5NJ_000783"
     CDS             complement(13437..13748)
                     /gene="yhbY"
                     /locus_tag="ACV5NJ_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HHY6484899.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13764..14873)
                     /gene="yqeH"
                     /locus_tag="ACV5NJ_000784"
     CDS             complement(13764..14873)
                     /gene="yqeH"
                     /locus_tag="ACV5NJ_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HHY6484900.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14870..15397)
                     /locus_tag="ACV5NJ_000785"
     CDS             complement(14870..15397)
                     /locus_tag="ACV5NJ_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HHY6484901.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15577..16362)
                     /locus_tag="ACV5NJ_000786"
     CDS             complement(15577..16362)
                     /locus_tag="ACV5NJ_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HHY6484902.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16355..17224)
                     /gene="accD"
                     /locus_tag="ACV5NJ_000787"
     CDS             complement(16355..17224)
                     /gene="accD"
                     /locus_tag="ACV5NJ_000787"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HHY6484903.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17225..18604)
                     /locus_tag="ACV5NJ_000788"
     CDS             complement(17225..18604)
                     /locus_tag="ACV5NJ_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HHY6484904.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18612..19031)
                     /gene="fabZ"
                     /locus_tag="ACV5NJ_000789"
     CDS             complement(18612..19031)
                     /gene="fabZ"
                     /locus_tag="ACV5NJ_000789"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HHY6484905.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19056..19532)
                     /gene="accB"
                     /locus_tag="ACV5NJ_000790"
     CDS             complement(19056..19532)
                     /gene="accB"
                     /locus_tag="ACV5NJ_000790"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HHY6484906.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19535..20770)
                     /gene="fabF"
                     /locus_tag="ACV5NJ_000791"
     CDS             complement(19535..20770)
                     /gene="fabF"
                     /locus_tag="ACV5NJ_000791"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HHY6484907.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20789..21526)
                     /gene="fabG"
                     /locus_tag="ACV5NJ_000792"
     CDS             complement(20789..21526)
                     /gene="fabG"
                     /locus_tag="ACV5NJ_000792"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HHY6484908.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21538..22455)
                     /gene="fabD"
                     /locus_tag="ACV5NJ_000793"
     CDS             complement(21538..22455)
                     /gene="fabD"
                     /locus_tag="ACV5NJ_000793"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HHY6484909.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22562..22786)
                     /locus_tag="ACV5NJ_000794"
     CDS             complement(22562..22786)
                     /locus_tag="ACV5NJ_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HHY6484910.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22839..23801)
                     /locus_tag="ACV5NJ_000795"
     CDS             complement(22839..23801)
                     /locus_tag="ACV5NJ_000795"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HHY6484911.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23811..24251)
                     /locus_tag="ACV5NJ_000796"
     CDS             complement(23811..24251)
                     /locus_tag="ACV5NJ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHY6484912.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6659 a         5428 c         4245 g         8027 t
ORIGIN      
        1 aatattcttg gattgcttct cgtgtttttt tcactttttc gctaagcttt tcgtttttag
       61 ccatatagaa ataaaaaatg atatcgatcg tagcaaattg ggccaaaagt gaattagttg
      121 ccgcacttcg attcgtgatt tcttttggtc tagatgtttg tacgagtaca tcggctatct
      181 tgctaagcgg gttatttcca aattgcgtca acgcaatgat ttcgatattc atgcttttcg
      241 ccagctcggc taatgcgact acatctgcac ttctacctga gtttgaaaca agccaaagca
      301 ctttcttttt ctcattgctg acaagttgcg ggagcaagac gtggatatct ttttcaaaaa
      361 tgattggctt accgacacga gaccattttt gcagaatatc ttctactact aaagaagagg
      421 cgccaacccc atataaaaaa acagtctctg ccgattccaa acactctacg actttttgga
      481 tcgtttcttc ttctagaata tctactgttt cttttatcgc tacttgtgca ttgttaccaa
      541 gtttgttttt gatagagtaa aaggactcat ttgcttctat atcactaaaa ccttcatcga
      601 ttccagtctg tagttctgtt gaaatcgtga ttttgaagtc cgtcagactt tgaaaaccga
      661 tttttttgga aaaacgtaca actgtcggcg cactcaaccc ggaagcttct gctaattcat
      721 tagctgtcat tgtagggacc ttctcaatat taccgataat gtagttggcc aacttttttt
      781 ctgctgaaga ataatcttcc atcatacttt caatacgact aattacgctc ataagttcct
      841 ccaccctttt aatgatagtc tatgtaagta taccatatca gacccttatt cacggtcgta
      901 tactggataa aaaacaaaaa aacaatccgc ccaacagaat atcttatccc atcaatccca
      961 cagattctat tgagcatctg tgtctttgag gaatacctgt tatctattgt tcggattgct
     1021 ttctatctac tactttttta caggaaattt acctattagt tttctctgct gtaatttttg
     1081 acttctgctt ccatagcatc aataaagata ttcaaatctt ctacagctgt ttctttactt
     1141 ccataacgac gtacgttcac tgttgcatct tccaattcct tgtctccaac aactaattgg
     1201 taagggatct tttgtgtttg agaggcacgt atcttatagc ccattttttc attacgatca
     1261 tcaacttcta cacgcaaccc cttcatttgc aaacgttctt tgatttcata agcataatcg
     1321 ccatgtgcat caacagaaac agggatgatt gttgcttgta taggtgccaa ccatgttgga
     1381 aaagcgcctt tgtatacttc tgttaaataa gcgacaaaac gttccattgt agaaacaatt
     1441 ccgcggtgga taactactgg acgatgcgta ttttccccgt cttctccaac atatgtcaaa
     1501 tcaaagcgct ctggcaataa gaagtctaat tgtatagttg ataatgtttc ttctgtacca
     1561 agagctgttt tgacttgaac atctagtttt ggtccgtaaa aggctgcctc gccttctgct
     1621 tcaaagtaat ctagttctaa ttcatccata gcggctttca gcattgcttg ggctttttcc
     1681 cacattgcgt catcatcaaa atatttatct gtattgtttg gatcgcgata gctcaagcgg
     1741 aaacgataat ctgtaatatt gaagtcagca tatactgcca ccatcaattc aagcgtacgt
     1801 ttaaattcat ctttgatttg atcaggacga acaaatgtat gaccatcatt caacgtcatt
     1861 tctcgtacac gttgtaaacc tgacaaagca cctgattttt catagcgatg catcattcct
     1921 aattcagcga tacgaattgg taattcacga taagaatgga tcgtgttttt gtagaccatc
     1981 atgtgatgag ggcagttcat tgggcgaaga acaagcattt cgccatctcc catatccatt
     2041 ggcgggaaca tgtcttcttg ataatgatcc cagtgtccag atgttttgta caactcaaca
     2101 tctcccatta ttggtgtata tacatgttgg taacctagac taacttcttt gtctacgata
     2161 taacgttcga tcgtgcgtcg gattgttgcc ccttttggaa gccagaatgg caaaccagaa
     2221 cctacttctg gggaaaccat gaacaagtca agttcttttc ctagtttacg atggtctctt
     2281 tcttttgctt cttcacggag acggatatat tctttcaaag ctttcttgtc aaaaaaggct
     2341 gtcccgtata cacgttgcat catttgattg tttgagtttc cacgccaata agcacctgcc
     2401 actgataata atttaaatac ttgtatacgg ccagtggatg gaacatgtgg tccacgacac
     2461 aaatcgatga attcgccttg ttgataagca gtaatcactt cttcttctgg caattcacta
     2521 atcagttcta ctttgtacgg atcacttgca aaaatttcta atgcttcctg ttttgaaaga
     2581 acacggcgtt cgatcggcag attttctttg acgattttca tcatttctgc ttcgattgcc
     2641 ggcaaatctt ctgcagttac ttggttttgt ccattatctg tatcgtagta aaaccctgaa
     2701 tcgatagctg gtccaacgcc aaagtggata tttggataga ggcgacgcat ggcttgggcc
     2761 atcaaatgag ctgctgaatg acggactaag ccaagtgcat cttcatggtc tggtgtgatg
     2821 atctcaatac ttgcatcttc ctcgattgga cgagtcaaat caacgagttt tccgttcact
     2881 ttaccagcta aagctttttt tgctaagctt tttgaaatgc tttcagcaat tgccaatgtt
     2941 gttgttccag actcaaattc tttgactgca ccatctggaa aagtaatttt tatcataata
     3001 gtttcctcct tgaaatgttt tgattgatca gatcatcaag ctgaaaaaca aaaaaatcct
     3061 agcatctctt gaaaagagat actaggacga aaagttcgtg gttccaccta atgttcaaac
     3121 gcaaatgcgt tcctcaagcg ttgtaacggt ccgaccgccc ttgcttcaac aagggtttcg
     3181 aaagtggtca tcctttggtt ttctcctgga aaagtttcag cctagctttt cctctcttat
     3241 cgaagactct caaattcagt tggctttctc atcaatcaat tatgtgttta tttaaccact
     3301 aataaataga aaaatcaagt gggaaactaa acttattttt tccattcccc atttacaccg
     3361 tatgttcctt ttttcatctc attgatgatg acatggatgt gttcttttgg tgcctgcgtg
     3421 tttttatgaa cagcttccgt gatatctttc atcatatttt ctaattgttc cggactgcga
     3481 ccttctacta attcgacatg tacgaatggc ataatgctct ctcctctttc tgatcgttct
     3541 tttttcaact cgattctatc atactatgtt tgtcttgggt actgaatcag gaaaaatggc
     3601 tggatctttt tttgctctca aggcactcaa aatagagacc gtatccacga cttcttgcaa
     3661 aacagctccg aataatgcgg ggattagtcc cgtacttgca attaacatca aaaaaataca
     3721 aataaagatg ccaatcagta cagactgttt cgctattctc atcgtatctc ttgaaatcac
     3781 tactgctttt gctacacgca agagatcatc tttcaagatg accgcatctg cactttcact
     3841 agcagctgta gaaccatgag cccccattgc aataccgata tctgcagcag caagtgctgg
     3901 tgcgtcattc acgccgtctc ctaccatcgc aacaggtctt tgatctttag ggatactttc
     3961 taaaatggtc agtttatctt gaggcaagct acgggcatgt acagaatcta attggacttt
     4021 ttctgcaatc gtttgagcaa tggcagatcc atccccggtg atcatcatca aatttttcac
     4081 atcaagtttt cttagctcct tgattgtctt tgctgcttct gggcgaatct gatccgaaaa
     4141 taggatcgtt cctgcaaaag atcgatcaat tgttacatag attgctgtat tggacgtttc
     4201 tatttcttca gctgaaacaa aagaggcacg tccaatcttt acttcatgat tattgactac
     4261 tgcttggatt ccttgaccgg tcacttcttt cagttgacta acaggataca gttggctgtt
     4321 tttcacgttg ttcactaacg aacgagctaa gatatggcta gattcctgtt ctgcactagc
     4381 tgcatacaga agtagctctt ctttttttat tccattgact ggctgaatcg cctctacttc
     4441 caatatccct ttggtaagcg ttcctgtttt atcaaaagca atcgtttttg tacgtgcgag
     4501 cttttcaatc gttgttccag tttttacaac gatcccgttt ttacttgaac ggctcattcc
     4561 tgcaactaaa gcaatgggcg cagctagaat cagtggacaa ggtgatgcca ctaccagtac
     4621 ctcagcaaat cgtaaagggt cttttgagaa ccaccaagaa ataccaccga tcaaataagc
     4681 aatcaacgtg aaagggactg cataacgatc agctaaacgg acaaatcggg caggcttttc
     4741 ttttgattct tcgactagtt taataatcat ttgatactgg ctgtctgcgg cacgtttttc
     4801 agcgatcatt tggatcgatc cgtctccatt taaagaccct gacataatct gatctccagg
     4861 tttcttggtt tccgggcggg actcacctgt caaagaagat tcatcgaccg aagattctcc
     4921 tttggtcact cgaccatctg cgggtacaag ttcgccaggc ttgactagta actggtcatc
     4981 aatatttatt tcatcgatta ctatatcctg caattttcct tctttgaatc gatgggcaat
     5041 tcttggggtg ttatctaaca aagactgcag ttctttaccc gctcgtttat tcgcgtaatc
     5101 ctctagacta tctccgcccg taagcatgat caatatcatc aaactagccc agtattcttt
     5161 gacggctaat gttgcaagga ttgcggtaat cgctaataca tctacgccat atcttcctga
     5221 acgtaacgtc ttgatcattt ccaaaaacat caagaaggcg agaatccctc cagtaaatgc
     5281 gacaatcgca aatgcaaggg ccggctggtg aaaaacaaat tcacttaata aagctatccc
     5341 accaataagc aaagtaagta aaaatttttt gaaatgtgtc attgtttttt ctcctctttt
     5401 atgtgactta gagaggttag taataaattt ccagctgcct ctttcatctt aagcatagtc
     5461 cttgactagc actttttaaa acaataggcc ttaattgaaa acaactatca ctatcagcgt
     5521 ttccaaaatt catcagttct acaaacaaaa atattcagca aggattactt tttcgattca
     5581 tcagttaaaa agtacctttt ttcgaaaaac tatgctatac ttttaattcg ttatttacgg
     5641 cggaaggagc aaacatgcga tgaatgagaa acaactagaa caaacgcacg tggatgaaac
     5701 gatcgaacta atccagatcg aacaaaatat gctggataca aaacaacaag aattgactca
     5761 ggaaatgaat cattcgacaa aagacacagc taatcacaag attcgagctg gttccaatga
     5821 agctttttat gaatctgctg ttgaatatcg ccaacatgag caggaattgc ttttgaaata
     5881 ccatactctt gaagcacagc aaaaacgcct gcaaacgttg tctgtcatga aagatagtcc
     5941 ttattttgcc cgtatcgact ttaaagaaga ccgtgaaaca gaaacattat atctaggtat
     6001 cgcttcttta agagatctgg cagaagaaac aatcgttatt gactggcgcg caccaattgc
     6061 caatctttat tatgaaggtg agattggtcc cgcattttat gaaactgaaa cagatagaat
     6121 agatgtcgat ctgcagttga aacgacaatt taaaatccaa gaaggacgta ttttatctat
     6181 ggtagatacg tcggaagtaa tcaatgatga ctttttgcta gagatattgg atgaatcatc
     6241 aagcaaccat atgaaaaaca tcgtctctac gatacaaaaa gcacaaaatg caatcattcg
     6301 ggacacaaca agtaaagtaa tgctgattga aggaatcgcc ggaagtggaa agacatccgc
     6361 tttgcttcaa cgtattgctt ttctgttgta tcataatcgc aagtggctcg aggctgaaaa
     6421 cgttcttcta ttttcaccca accatttgtt ttctgattac atctcgactg tattgccttc
     6481 attaggtgaa agcggcgtgc ctacacagac attcaaaaat tatttggtgc agctgctgcc
     6541 agagttttct ttactagatg aacagcaaca agaaagtggt tttttgctag gggaaaaaga
     6601 tccgatacaa gtgatgaaaa gcggattgac cttagtcgat cagattgatc gctatatcca
     6661 aggcatcaca aactacggtc ccttgttccg tgatatgaaa ataaatggcc gtacgatcct
     6721 atcaaaggaa tcgattcgcc aatggtataa agaaacaaat gaacaattac ctttacatca
     6781 tagactttct ctattacaaa cgaagctcct gaaaaaatta ggcggtctgc aaaaagacga
     6841 aacacgtcag caatgggtca aagatctggc ggaagaacag ctccaagaac tatacgcaac
     6901 agatccaaat cttgaatata cagaacaaaa ggagcgttca ttacgtaaaa aactggccaa
     6961 ccaaatcgta cgtaaaagat tccgtcgaat ccatcgaggg atcatgaatt atcaatttgt
     7021 caatataacg aaacaatatc tccactttct acaaactgtg ccactttctc ttctaacacg
     7081 acataaaatc agcgagacca ggtggcagga acatttgaca actgttcgta cacacttgcg
     7141 tcaaagagaa ttgcgtcaag aggatgctgt actattcttt ttactgatgc gccgaacata
     7201 tccagtgacg gttacacaca aagctcgcta tatctttatt gatgaaatgc aggatttcgc
     7261 accatcacaa gtggcattgc tgcaagcctt gttcccaaaa gcaaatttga ctttctgtgg
     7321 agatctaaat caaaatgtct ttggaaatga aacgatcgtt ggatcattgg aagacctttt
     7381 cgtggaccgt gaagtcactc gtttccaatt aacgacaagc tatcgctcca caaaagaaat
     7441 cactgatttt gctaatcatt ttattgtgaa agaacatcaa gtagaaacaa cagcaagaaa
     7501 aggagacctt cctttgctca tccaaggaac aacagatgat atcaaattga attggttagc
     7561 agataccatt cgaacgactc agggaaaagc ccggtattgg cgtacagcaa tcatagctaa
     7621 aacacaagaa gaatgcgaag ctctttatgc tcaactgcca gacgagttga aacaacaagt
     7681 ccagctgatt ttaaatgaga gtgactttat gaagcgttcc attattttac ttccagcctt
     7741 tttagcaaaa ggtctggagt ttgaccgagt tttcttgtgg gatgtagatg cagaacattt
     7801 ttcttctcag caagacaaat tgatccttta caccatgtgt acacgtgcga tgcatgaatt
     7861 gacccttttt acctccaata atgaaagtcc tttgttaaac aatatggata tttcatctta
     7921 tgagaaaaaa gtactgtaag taaaaagaca actgctatca ttaatgttag cagttgtctt
     7981 tttatttaca ataataattg cagaaaaagt aattcaatca tttaaggtca tttattctat
     8041 atataaatcc aaatctgttt caggatattt taatttgaca agcgaaataa tcattttcag
     8101 ttcttccatg ttttctggat attctgggtc aaataccttg ctatgacggc caatcccgcc
     8161 attgtcgatc aatcggtaaa ttcgattcca ttcaatgaat tggtcaattg attggaaatt
     8221 gctgattgga tagccgccac aatccaaaat ctctgataca cgaaatgcta ggtatgcact
     8281 atcgatcact ttattttttt tcgtgtctat cttctcattt ctttcgataa gaattttttc
     8341 aggacgatgt ttcaagctcc actctaatag tagaaagaaa gcctccatcc gatactcttc
     8401 ttcaaataac cgaaaggctg aatcagaacg acgaaaaagc agcggaatca gataattttg
     8461 tctataaatc agcattggtg cgcggtccac catacactct aaataacggt tcagataccc
     8521 tgtaccgaca tagtttccta atcgatagcc ttcaacgccg aatcttgcgt atttttgcgc
     8581 taattttgaa caatcagtta cgttcacagc taactcttct actttgcggt aaccgcttaa
     8641 gatacgttca aaaggttcta aagtgatttt tgcttccata ttcttccccg tccatcttcc
     8701 acatacttca tatcatttca catgatactt atgctctcat cataccacag gttgaattag
     8761 aaaagaaaat aaatcacaca aaaaagcaag acaatctgtt agtgagattg tcttgctttt
     8821 cgaaaaagac tgaatacttt tcgttaaaac tggttgctta gtcttctaat gccacattat
     8881 ggaacacatg ctgtacatct tctagatctt ccagtgcatc aatcattttt tcaaatgttt
     8941 cttgatcttc tggagatagt gttacctcgt tttgagggat catttggatc tcagcgattg
     9001 aaaaatcagt gattcctttt tcttttaatg cttcttgtat actatggaaa tccgctactt
     9061 caccgtaaac gatgatttgt ccttcttctt ccattacatc gcgaacatcg atgtcttttt
     9121 ccattaggta ttcaaggatc tcgtcagcgt cttctcctgc aaaagcaaag attgctgtat
     9181 tgtcaaacat gtaagaaacc gctccggaaa cacccatgtt cccgccattt ttcccaaatg
     9241 ctgcccgtac gtctgctgct gttcgattca cattgttcgt caatgtatca acaatcacca
     9301 ttgagccgct tggtccaaat ccttcatagc gcaattcaga gtatgtttca tctcctgcac
     9361 cttttgcttt ttcgattgct cggtcaataa tatgttttgg tacattataa gttttagcgc
     9421 gatcaatgac gaaacgtagt ttttggtttg catgcggatc aggatcacct gattttgctg
     9481 ccgcgtagat ttcaatccca aattttgcat aaatccggct attattagca tctttagcag
     9541 ttttctttgc tacgatattt gcccatttac gtcccatatt ttcacttcgc tttctgattt
     9601 gatagtttca taacgcaaaa acgtcctgtt ccattatact tgtgaatcac gattctgtga
     9661 agcttttttt ctttcttcct tcattcttct catttttagt caattatcta tactcggatt
     9721 gggatttgcc cgaaattctg ttccggaata ttgcctccca attgatagat ttgatcactt
     9781 ctaactaaca gttccgcttc ttgtgcctct ttttttccga ttcgggaata aatagaaatc
     9841 tctttttttt cttttaagaa tcttcttcca gcatcggtaa atccaagtag tcgaagataa
     9901 tttttatttt gttggacagg aatctcttgc tttttgatat tcagcaagac ataacaagat
     9961 aatcgttgga tccgtgtcca tgtatatctt tttgttttta cctttgacac aaaatcctca
    10021 aaactatcag cagttgcagc tgctgttttc atgcgcgatt ctattccttc ggtcatctga
    10081 tagatatctg tcagttccag caaagtagaa gataacaacc gataacgcaa atacggccaa
    10141 taagattccc aagtctgcag cgaacaagtt gaaagatctt cttctgtctg aggcggcaca
    10201 agtccagcta ttctttttcc ttcttctaac gcatggcgaa tggctgttgc actggctatc
    10261 ctctgatctg tgatgatttt ttcatgatag cctgcacttt tacgtcggat cggatgtaat
    10321 tccatagggt ggacatactt ggcattttcc tttgcataac ttaatcccaa gatatgattt
    10381 ggcgaagaaa aatcaagcgt aatatcggga tacagactgc gaaaaacctc agtcattttt
    10441 tgcggataac tatacgtttg atcagacagt tcttgaaatg cctcttctat cctatcttga
    10501 ttctcttgga caaagcgtcc gaaactgctg taatcaaacg aagtctcttc ttctgttcca
    10561 aaacacaaac tgtcacagtc cagtgtttgc aagattttta ctgcaccttt agcaaaatag
    10621 tcagctgact gaaccgccca ttcgacggga agctccacta ctaaatccac accattttgt
    10681 aaagcttcat tcgcgcgatg ccatttatcg atgatagccg gttcgcctct ttgcaaaaaa
    10741 ttcccgctca ttacagcaat gacgacctct gctcctgtta tttttcgtgc catttccaca
    10801 tgatagcgat gtccattgtg aaaaggattg tactctacga taatcccgca gcttttcatg
    10861 cgcatcctcc ttcatagtta tcgataaaaa agaaatagag acaagtaaaa aaaggcaaaa
    10921 atcggaaaaa atacccgaac tatataaact attctcgcaa acacaatact tctgcgacga
    10981 aaagctgcac tgcaagagca atgcctatat cgttcggata tataatttat gttgaagatt
    11041 tattcttttc ttgttaactc gagcaggcaa aaccgctagt ctttgtatgc tacaaagaac
    11101 cagcgctttg atttctctga tgctgcttcg tctgtaaaat cagaaaatgc ttcgactttc
    11161 gaaaagccgc tgttttccag cattcttaaa taactttcca atggataggt acgctcttga
    11221 tgtagttcat cttcacgaat aaatcgacca gacccttctt gttcttcaac gaaaaaggta
    11281 agaaaatgtt cgatgctgtg tttctcgctt cctggataac tatcccacaa aaaagcaaaa
    11341 ttatcccctt gataatgata gctatattct ggaaaaacct catctacttg ataggtggag
    11401 tgtacgtcaa ataaaaatcg gccattttct tctaacgctt gatagacttc atcaaacact
    11461 tgctgtacct cttgtctatt tttcatatag cacagcgaat cagaaaaaca ggtaattgcc
    11521 tgatattgtc cgatttctga taaatcgagc atattgcctt ccacaaattg gatcgatgcg
    11581 tcttcttcat atgctcgctg gctggcaatc atcaacatct cttctgaaag gtctaaccct
    11641 gtcactttat atccatcttt tgcaaaggca accgctaatg ctccagtacc gcatgccagt
    11701 tccaaaatat tttttgtgtc ttcttgtaaa tgacgtttcg aaaaagctaa ccattgctga
    11761 taaagtgttt cgtccatcac ttcatcgtaa atgaaagcaa atgtttcata agccatatgt
    11821 taatccaacc attcactaag atcgactagc ggagcatctg accatagttt ttccagatta
    11881 tagaaatttc gttctggcgc atggaaaaca tgtacgatca catctccaag atcgatcaat
    11941 acccattttc cgccttcttt tccttcaata cgttttactt catatttgtt ttcttcttct
    12001 ttatcaatga tttcttctgt aattgcgttg atttgacgtt cgctatttgc tgaacagatc
    12061 atgaaataat ctgccaacaa ggaaacattt cgaacgtcta aagcgataat gtcttctgcc
    12121 cgttttgaat ctgctgcttc aatcgcaatt tttaattgtt ctttactatc tatgatgatt
    12181 tcctcctaat atgagcagct tatttttttg ctacccagta attatatgtt tcgattgttt
    12241 ttggataaat ctgttgttct tgcgcaagca aatgggccaa tgtatgtttt gtttcaaatg
    12301 ctacagcttc gtctagatca gcccatgcaa tggctcgtgc atcttgtacc ccaggaaaat
    12361 ctcgacctgg ttcaatatag tctgctacat aaatgatttt gtcaaggaga ctcatcttag
    12421 cagccccagt cgtatgcacc cggattgcat gaagaatctc tgcatcggtg attcctaatt
    12481 cccgctctac aaacgaagcg ccaacaagac catgccagat tgcattatta taattcaata
    12541 actccggatc aaaaccgtct cttacgatta ccattttgaa ctcctcatct ggtcgttctt
    12601 ttgcataatc atgtgtcaat gcagcaatac tagctttttc aggagaagca ccgtattttt
    12661 tggcaagagc cacggctgtt tcttctactc caagcacatg cttgaagcgt tgttcgctca
    12721 ttctcatctg tattttttgc atcaggtttt ctctagaata agcagtatat tcattactat
    12781 agtccatatt catacaaccc ttcattttga atataatcaa ttactttatc tggaacaagg
    12841 tagcggatag aacagccttc tttgatcttt tgacgtattt tcgtggaact gatgtcgatc
    12901 tcaggtacat ctacccaaat tacaggatat ggagtatccg ttgtgtatcc tggtcgacgt
    12961 atacccacaa agttgaccat agaagtcagc tcatcgattt tataccattt tggcaaatat
    13021 tctaccatat ccccaccgat aataaagtaa taatctgtat ccggattgtt ttgcgtcaat
    13081 tccttcatag tatcataggt gtaacttttc ccaccacgag ctaattcaat cgtttcaatc
    13141 tgcaaaaaag gattgtcttc caccgctaat tccaacatgt tgagtcgatg ttttgcatca
    13201 atcgtctgtt tctcatcaac atgaggtggc tgataagaag gcatcaaaaa tacgcggtca
    13261 agacctaaat tccggccagc ttgttcagcc atcactaaat gtgcaaggtg aacgggattg
    13321 aatgttccgc ccaagatccc tacttgcttt ttcttctcta agaattgagg catttcttca
    13381 ggaaataatt gtgaacgaac aaatgtctta gcttgcgtat tcatcatggt cactccttat
    13441 attgctttta cttcttttga tagcctttga tacttttctt ttgtggaagg tttgtataat
    13501 acaataacac gtccaatgat ttggacgatt tcgcaatcta tttctttttc taatacttga
    13561 gctgcttctt ctgcaatctc atctgtattt tgaagtaacg atactttgat cagttctctt
    13621 ttttctaaag cttcttcaat ttgaacgatc attgctgaat tgatcccacc ttttccgatt
    13681 tgaaaaatcg gctgaagatg atgcgctttg ctacgtaaaa aacgcttttg ttttcctctt
    13741 aaactcataa ttttccttct tccttaaatc aatgctttgc gtttcaatac atctacaccc
    13801 tttggcgccc agcccgcaat tactactggc gctgttacag tgatccagcc tagaccggca
    13861 aaaacgatgt ctgttttttc tttcacagaa aactcaaaac gaacaagctc cggaaatgcc
    13921 tcgacttcat caggacgagg aggctgtaat aatccaccta catgtttttg ataaaattcg
    13981 tctgcttttt ccagtttcgt tcgatgaata tttacatcat ttgacacata agcaataaat
    14041 gaagcacgat tgccgctgat aaaatcaaat cttgcaagtc cgcccaagaa aagtgtttgc
    14101 ccttcattca gctgataaac tttaggtttg atttctttta acggtgcaat gatccgcaaa
    14161 tcttttttac ctaagtagtg cgccatttga tggcgatgga taattcccgg tgtatcaatc
    14221 aaaaaatgcc cgtcatctaa aggaatctcg attttatcca aggtcgttcc tggaaactgc
    14281 gaagttgtga ttacatcttg tactcccgct gtatttttga tgatttgatt gataagcgtc
    14341 gattttccga catttgttac tccgacgata tagacatccc gtccatcgcg gtattcttcg
    14401 attttttcta atagttccgt catctcatga gatttcttag cactagttaa taatacatct
    14461 actggacgaa ttcctgcttc gtaagcacgt tccttcatcc attgtgttaa tttagttttt
    14521 ttcaatgatt taggtaatat atccactttg ttcccaacta aaagtactgg attgtcaccg
    14581 acaaaacggt gtagtcctgg gattagtgaa ccattgaagt caaaaatatc caccacattg
    14641 acgatcaatg catctttttg ccctaattca ttcagtaagc gcaaaaagtc gtcatccgtc
    14701 aatgagacat cttgggtctc attataatgg cgtaatctga aacaacgctg acaatagact
    14761 tccccggaag ctaaaccttt ttccaaagcc gtttgcggcg tgtatcctaa ttcaccgggt
    14821 ttgtttgttt gtataacggc accacagccg atacattgta attcttctgt cattcgattc
    14881 cacctcgcca gcccatatca gggtgttttg ctaatagata ggccataatt cttttttccc
    14941 aaaaacgatt aaattgtgtc ttccaactat ctgttgcgat aatgggtttt acaaggatgc
    15001 tacgtatccc tgcacgattg gctccacgta tatcagtcat aatctgatcg cctaccatta
    15061 ctatctcatc atctgctaaa tccaattgtt tctttgctat gttgatccct cttgctaaag
    15121 gtttcattgc tcgtgcaaca tacaaaagat caaatttttc aatcgctcgc gcgacacgtt
    15181 ttgaattatt gtttgaaaca acaacaacag ggattcctgc atttttcatt tctaaaatcc
    15241 aagtaagcaa ctcttctgtt ccatctggat tattccatgc aatcaacgta ttgtctaaat
    15301 cggtcaatac tgctttgatg cctagattct ttaattgggc aggtgtaatc tgatagatgg
    15361 cttctaccat ccatgttggt ttgtaatttg aaaacatgct gtctcctcat ctgcaaaaaa
    15421 tgaaggagca cataaagtac tcctcaaccc cgtattatac tgcatatcag caaaaaaaac
    15481 tacctctata ctcgttcgtc tttcagaaaa agcatttctt tacaaaatga ccaaaaataa
    15541 agattgatca cagaaaaatt tagaaacaaa aaacaactaa tattggcgga acctcttgta
    15601 tcgtttttcc agtaatttat gctgatctaa tcgaacaagt tccgtcaatc tttcaatcaa
    15661 cgcttttttc ataatgtgtg cgactttata tcgtggcaac tgctcgccgt tgaacgtttc
    15721 aggaataact cgatcaacga ttcctaactc gcgtagttct ggcgcagtaa ttttcatcaa
    15781 ctcagctgct tctgcggcgc gactaccgtc tttccacaaa atcgacgcaa atccttctgg
    15841 agaaagcaca gcataaatcg tttcttccag catccagact tcatccgcta aacctagagc
    15901 aagggcaccg ccacttccac cttcaccaat gatgatggcg atgatcggta cctttaaatc
    15961 agacatttcg agcagatttc tggcaatagc ctctccttcg ccgcgctctt ctgctcctac
    16021 accgcaatat gcacctgctg tattgatcaa ggtaatcacc ggtctattga acttttccgc
    16081 ttgtttcatc agtcgtaaag cttttcgata gccttcagga tggggagaac caaaatttct
    16141 gcggatattt tcctgcagat cccgtccttt ttgaataccg atcactgtta ccggtttgcc
    16201 atctaatcga gcgattccac caacaatcgc ttcgtcatct gcaaataaac gatcaccatg
    16261 gcattcctcg aaagaatcga agatctcttc aaaaaaatcc aagcttgtcc atcgttcttt
    16321 agctcttgct aactgaacga catcatgtgc tgtcttatcc acgatctatc catcctttct
    16381 gtgtatgcaa ttcaagaagc gtatggattt tctgtttcaa ttccatgcgg ggaacaatct
    16441 gatcgacaaa accatgagat aataagaatt cagccttttg gaaatcttct ggaagttcct
    16501 gcttgatcgt ctgttcgatg acgcgtcttc cagcaaaacc gatcaagctt tggggttctg
    16561 ctagaataat atcgccttcc attgcaaaac tggcagttac tcctccggtc gtcgggtccg
    16621 ttaaaacagt tagataaaaa agaccttcat tgctatgccg tttgacagca gcagagattt
    16681 ttgccatctg cattagagaa aagattcctt cttgcatccg tgcgccacct gaagctgtga
    16741 acaagacgac tggaagactt tcctttgttg ctcgttcgaa taatctggtg atcttttctc
    16801 cgacaatcgt tcccatactt cccatgataa aattaggatc catcactcca ataatgaagg
    16861 gaatcccctg aatggttgct ttaccagtga ggactgcttc atgaagtcct gttttatctt
    16921 gcattttttt aattttttcc gtatattcgg gaaatgctag cggatctttg gttacaagat
    16981 ccgtatccca ttcttcaaaa cttttttcat caataatcaa agctagacgc tgccatgcac
    17041 cgattcgaaa agcgtatccg caatgagggc atactttttc ttcaccgatg tctttcgtat
    17101 aaaggatatg tttgcaattt ggacactttg cccacatgtt atcaggaaca gatggcgagt
    17161 tagcaggttt gctctctcta ttaggattga tccgaatata tttctttttc ttaaacagag
    17221 ccatctatgc gcctcctatc tccgtttctg gtgtccaatt cggtaaaaat tcttcttgta
    17281 aaaatgctgt gctgtaatca cctgctatga ctgcaggatg actaatcaaa tccatctgaa
    17341 attctgcgtt cgtgatgatt ccttctgtga taaattcact taaggctcgc tgcatcttca
    17401 ttaatgcttc aaaacgcgta tttccatgga cgattatttt tgcaatcatt gaatcataat
    17461 atggcgggat cgtatatccc gggtaaactg cactgtctac tcgcagtccc atcccacctg
    17521 ctggaagcag taagttttgg atcttccctg gagaaggagc aaaatggaaa gcaggatttt
    17581 ctgcattaat ccggcattcg attgcgtgtc cttggaatac tacttcttct tgacacacat
    17641 tcaaaggctc accggctgct atctccacct gtttttttac taaatcgatt cctgtgacca
    17701 tttctgttac tggatgttcc acttggattc gagtattcat ttccatgaaa taaaaatcgc
    17761 cttcttcatc catcagaaac tcgatcgttc cggcattttc ataattgact gctttagctg
    17821 ctcgaacagc agcgtctcct agtgcttctc tttttgtttg tgagatcact acagaaggag
    17881 attcttccag tactttttga ttgttgcgtt gtaaagaaca atctcgctcg cctaaatgaa
    17941 tgacatgacc aaatcgatca cctaagatct gaacttctat atggcgagca ggataaatga
    18001 ttttttccaa atacatatca tcatttccaa atgccgctgc tgcttcttgc tgagctgaag
    18061 aaaaatgctg tgggagctct tcttttgatt gaactttccg aatgcctttc ccgccaccac
    18121 ctgcagctgc tttcagcatc actggataac caattctgtc tgctatttcc aatgcttctt
    18181 ctactgtaga taatacacca gtacttcctg gaatgactgg gacatttgct tcttgcatca
    18241 attgacgcgc attgatctta tttcccattg catcgatcgt tgcactttta ggaccaataa
    18301 atgtgatatt gcactcttca cacatggagg caaatcggct attttcagat aaaaaaccaa
    18361 atcctggatg aattgcttcg gcttttgtca cgattgcagc gctcagtact tcctgaacat
    18421 tcagatatga atcaacggct tttgccgggc caatgcagat tgcttcatct gctaactgtg
    18481 tatgaagtgc ttcggcatct gcttctgaat atatcgctac ggtttggatc cctagttcgc
    18541 ggcatgcacg aatgatacgt acagcgattt caccgcggtt agctatcaat actttagaaa
    18601 acatatttca cctatccaat cataaaggtt aattccgctt cggctacttt tttaccgtct
    18661 actttagcga ttcctttgcc tattccggct gaagctttca ctttcaatat ctcaacttca
    18721 aggtataagg tatctcccgg tgtaactttc ttacggaatt ttgctttgtc caatccgccg
    18781 aaataagccg tctttccctt gaattgttca agagaaagta atgcaactgc tcctgcttga
    18841 gccattgctt caacaatcaa tacccctggc ataacaggtt cttctggaaa atgtccttga
    18901 aaaaagggct cattgatcgt tacatttttc ttagcaacga tccgttctcc ttcaaccatt
    18961 tcttccactc gatcaatcaa aagcattgga taacgatgtg gaatgatttc ttttatttct
    19021 tgtatgttca ttgttttccc tctttcctgc tttgcttatt tttgctgaat cctaaagagt
    19081 ggctgtccat attcgacaac tgactcgttt tctacaagga tttctgtgat cactccgtcg
    19141 gcctcactag ttatctcatt catcagtttc atcgcttcaa taatgcagat aacttctcct
    19201 ttggcaactt gatcacctac ttttttgaaa gctggtttgt cgggcgctgg ttgcagataa
    19261 acgattccca cgattggcga aacaacctcg attccatcaa atactgccga ttctttttct
    19321 ttcattattt ctggagcagt tgttgcttgt tcaggtatga cattgactgg ctccgaaact
    19381 ggcaacgttt cgtttgtttg aaccatattc atgcctcgac ttgttttatt tttattcata
    19441 tacaattcaa attgaccttc acgcaaatcg aattccgtta gcgaggattg atcaaattga
    19501 ctgactaatt ctctgatttc attgatatcc aaaattattc ctcccagcgt ttcatgcaca
    19561 gaaccgcatt atgtccgcca aaccctaatg aattactcat tgcataagta aaacttgcag
    19621 gtttgctttc gtttttgaca actgtcaagt caatctcagg atcttgtttt tctacattga
    19681 tcgtaggtgg gataaattga tgttctaatg ccagcaaagt tgccaaggct tcgatcccgc
    19741 cggctgctcc caataaatgt ccagtcatcg atttcgtgct gctgacgtaa gtatctctga
    19801 aattttcacc taatgcgatt tggattgctt ttgcttctgc ctggtcattg gctggtgtac
    19861 ttgttccgtg agcattgata tatccgactt gttccggctg tacaccagct tcttccatcg
    19921 caagcttcat tgctttagat gcaccagaac catctggagt aggagctgtc atatggtaag
    19981 cgtcacagtt tgcaccgtat ccaacgattt ctccatagat tttggcaccg cgagcttgtg
    20041 catgttctaa agattcaaga actagaacgc ctgctccttc acccatcaca aaaccattcc
    20101 gattttcgtc aaacggaatc gaaccacgat taggatcagt tgattctgag agtgctgtta
    20161 aagctgcaaa tccagcaatc cccatctcac aaatcgttgc ttctgttcct ccagcaagaa
    20221 tgacatcaga atagccatgt ttgatgttac ggaatgcttc cccaattgaa ttcgttccgc
    20281 tagcacaagc tgttacgata gctgtacaaa taccttttgc acctacacgc aaagcaatat
    20341 ttccagctgc catgttggca atcgccattg gtacgaacat tggagaaact cgttttgcac
    20401 ctttttcatg tagacgaata acttggtttt cgattgttgg aagaccaccg atcccagatc
    20461 cgatgatgac gccaaaccgg tcttgattca ttttttcttt atctaaaccg ctcatctcca
    20521 ttgcttctaa agcagcgtag attccataga tcgaaaacat atccattcgt ttgctatctt
    20581 ttttaataaa atatttatcg taagggaaat ctttgacttc acccgctaag gtaatccctg
    20641 tttctgaagc atcaaattta gtgatcggtg cgatgccatt cgtcccctct tttaagctat
    20701 tcaaaaagct gtctgcgtca tttccaatag gtgaagtgac tccatagcca gtaattacca
    20761 ctcgattcat ttgtttgctc tccttttctt atccgtgcat gaccaatccg ccatcgacat
    20821 tgatgacttg tccggtgata tatggatttt gtgctaagaa aactgctgtt tgtgcaatat
    20881 cttctacttg tccgaaacgc tgcatcggaa tttgcttttc cgcttgttcc ttgactttgt
    20941 ctgctaaaac ttccgtcata tcagttgtga tgaatcctgg cgcaatggca ttacatgtaa
    21001 tgcctctagt tgcagcttct cgtgccacag attttgttaa tcctactacg ccagctttac
    21061 tagctgcata atttgcttga ccaatatttc caatcaaccc agatacacta gagagattaa
    21121 tgatggcccc ttctcgctgc ttcatcattt ttttcaatac gtgctgcgtc atattgaagg
    21181 ttccaatcaa attgatatcc agacattttt tgaaatcttc tgcatccatt cgcatcacaa
    21241 gtttgtcatt tgtgattccg gcattgttta ctaacacatg gatcgatcca agcttttctt
    21301 ctgcttcttt gatcatttgt cccgctttct cataatcaga gatgtcaccg gaaactccta
    21361 cgcattttac accgaatgct tcgatttctt ctatcttttc ttttggaatc tctccgcgac
    21421 cattcaaaat gatatttgct ccagcttttg caaaagctaa ggccatcgct ttgccgattc
    21481 ctcttgtact acctgtgata aatacatttt ttccttttac atccataacg ttcctcctta
    21541 tagtattgct attgtttcat caaatgtctg cttattctca acacgcagaa ctggtattgt
    21601 tttatcgatt tttttcataa atccgcttaa tacttttccg gggccgactt cgatgacttg
    21661 ttcgatgccg attgttttta acttatggat actctcatag aaacgaacag gttgcatgac
    21721 ttgctgttca agtagtcccg cgattgtttc tttcttcatt atttccgttg ttgtgttgct
    21781 tatgatagga aaagaaggtt cagagaattg aatctgactt aggtccttcg ccagtttttt
    21841 tgctgccggt tctaaaatag ctgtatgaaa aggcccgctg acatttagag ggatcattct
    21901 tttgaaccct ttttctttta atagtaaaac agcttcgtct actgcttttt cttcgccacc
    21961 aatcacgatc tgctgaggtg tattataatt tgcaggagat acgatgccgt acttgctagc
    22021 ttcatgacag ctttcctcga tcgtttcaat cggcgcattc atcactgcaa ccattttccc
    22081 gcttccagct ggggcagctt ctgtcatata agcgcctctt tttgctacta acgcaactgc
    22141 ttcagtgaag gatagcgcat tactagctac taatgccgaa tattctccta aactcagtcc
    22201 taaagctgca tctgggatga ttccatgctc cttcaataag cgataatacg cgatgctgac
    22261 tgttagaata gctggctgtg tatattgcgt ctgatccaaa cgttcatttt ctgtaaagca
    22321 aagttcagcc atatcgtagc cgagaatctc acttgcttca tcaaatgtct gctttacaac
    22381 ggcttcttca tataaatctt ttcccatacc ttgatattgt gcaccttggc cactaaataa
    22441 gaatgctgtt ttcatgtttt tctccaatat atccagaata tatgtttgat tcttcctatt
    22501 caaaaaggcc gagacattcc tatttcagga aatgtcccag cccccaacct aattttaatg
    22561 attatgcagt ttgtttttct acgtaagtca ctaaatcttg aaccgttttg attccgtctt
    22621 ctgtttcgat tttcacgtca aattcatctt caatttcatt gatgatttgg aataaatcca
    22681 agctgtcagc ttctaagtct tcttggatgt cagtagtcaa tttgatttct tcagtttctt
    22741 ttcctaattc ttctgcgatg atttcttgaa ttttttcgaa taccataata atatattcct
    22801 ccgatattgt ttgcttttta tttttataat ttaacagtct acaactccat gagaatactt
    22861 ccccatgtta aacctccgcc atagcctgta aatattactt tctgctgttt tcctaaggtg
    22921 atctttccac tagaaactgc ttcatccaat aagataggga tgcttgcagc agatgtattc
    22981 ccatatttat ccatattggt caggaacttt tcttgcggca tctttacttt tcgggcaatt
    23041 ttctcaataa ttctgaggtt tgcctgatgc aaaagcagat aatcaactgg tttgtctttc
    23101 atgacttctt ggatattgtt tgcaacgtct cgtacggcaa aatcaaaaat atctctgcca
    23161 gtcatttgca aataatacga accttctgaa tcttctgaat agaacggatt tttattttgc
    23221 tggtagccag cggtcaatga gctacttctg gtaccgtcag ctgctaattt ttctttcagc
    23281 aattttttct gtggattcgc ttctaagaga atcccacctg ctccatcgcc aaacaacaca
    23341 gcagtcgtac gatcagacca atcgattagc ttagatagtg tttcaccacc aatcaccagt
    23401 cccttagttg agccgcagcg gatcattttt tcagccatgc ttaacgcata aacaaatcct
    23461 gagcacgcag cactgatatc aaaagcgata gcatgagttg ctcccaattt cccttgtact
    23521 tgtgctgcta cagatggtgt cgtgtaatcg ggcgacattg ttgctactaa aataaaatcc
    23581 aaatctttag ggtttagatt tttctgtttt atcaattttt ccgccactaa aatgcataaa
    23641 tcagaagtct cctgatttgt tacacatctt cgttcacgaa tacccgtcct tgacgaaatc
    23701 cattcatcag aggtatccat catcttggaa agatcattat tagtaataat cttttcagga
    23761 aggacgctgg ctgttgcagt aatcattcca aattgttcca ttaaatatcc tcacttatag
    23821 tcttgcaaga agtcgtgcaa gtttttcagt gcatccagca aggcatgctg ttcctccgta
    23881 gccattccgt ctaagatgtt cttgaccatt tcacgatgaa aatgctgatg gacccgaaat
    23941 agtaactttc cttttttcgt tagtcccaac tttacaacgc ggcggtcatc ttcactgcgg
    24001 atacgttcga catagccttt ttttactaac ttgttaatag cagtggtcaa ggtcccaaca
    24061 gtaattgata gttctttggc tacttctgaa gttgtttttt tcttgtacat cccgattgcc
    24121 tcgatcgtgt gcatctctgt gattgacaga tctttgaatt gagatttttt cagctcagac
    24181 tcttcgattg tcaaaatatc attgaagaca ctaacaagat aatcatttac tgtttttaaa
    24241 tctggttcca tgttccaccg tccactaatt agtttgatta tcaaatcttt tgatgttcaa
    24301 actatatctt aacttttttc aaaatgcaac cttaaaagat cattttttta ctatatgag
//
LOCUS       DBKOIJ010000025        24333 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000025 DBKOIJ010000000
VERSION     DBKOIJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.025"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(133..2478)
                     /locus_tag="ACV5NJ_000797"
     CDS             complement(133..2478)
                     /locus_tag="ACV5NJ_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HHY6484913.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="ACV5NJ_000798"
     CDS             2757..3659
                     /locus_tag="ACV5NJ_000798"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HHY6484914.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="ACV5NJ_000799"
     CDS             3870..5645
                     /locus_tag="ACV5NJ_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HHY6484915.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7293)
                     /locus_tag="ACV5NJ_000800"
                     /pseudo
     CDS             complement(5711..7293)
                     /locus_tag="ACV5NJ_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
     gene            7544..7768
                     /locus_tag="ACV5NJ_000801"
     CDS             7544..7768
                     /locus_tag="ACV5NJ_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HHY6484916.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7910..9661
                     /locus_tag="ACV5NJ_000802"
     CDS             7910..9661
                     /locus_tag="ACV5NJ_000802"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484917.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9661..11445
                     /locus_tag="ACV5NJ_000803"
     CDS             9661..11445
                     /locus_tag="ACV5NJ_000803"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484918.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11780..12632
                     /gene="licT"
                     /locus_tag="ACV5NJ_000804"
                     /pseudo
     CDS             11780..12632
                     /gene="licT"
                     /locus_tag="ACV5NJ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12729..14615
                     /locus_tag="ACV5NJ_000805"
     CDS             12729..14615
                     /locus_tag="ACV5NJ_000805"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HHY6484919.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14656..15558)
                     /locus_tag="ACV5NJ_000806"
     CDS             complement(14656..15558)
                     /locus_tag="ACV5NJ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HHY6484920.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15788..17191
                     /locus_tag="ACV5NJ_000807"
     CDS             15788..17191
                     /locus_tag="ACV5NJ_000807"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HHY6484921.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17191..18624
                     /locus_tag="ACV5NJ_000808"
     CDS             17191..18624
                     /locus_tag="ACV5NJ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HHY6484922.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18769..19695
                     /gene="trxB"
                     /locus_tag="ACV5NJ_000809"
     CDS             18769..19695
                     /gene="trxB"
                     /locus_tag="ACV5NJ_000809"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HHY6484923.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19908..21635
                     /locus_tag="ACV5NJ_000810"
     CDS             19908..21635
                     /locus_tag="ACV5NJ_000810"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HHY6484924.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21651..22001
                     /locus_tag="ACV5NJ_000811"
     CDS             21651..22001
                     /locus_tag="ACV5NJ_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HHY6484925.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22095..22955)
                     /locus_tag="ACV5NJ_000812"
     CDS             complement(22095..22955)
                     /locus_tag="ACV5NJ_000812"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HHY6484926.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23047..23475
                     /locus_tag="ACV5NJ_000813"
     CDS             23047..23475
                     /locus_tag="ACV5NJ_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HHY6484927.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23637..23813
                     /gene="rpsU"
                     /locus_tag="ACV5NJ_000814"
     CDS             23637..23813
                     /gene="rpsU"
                     /locus_tag="ACV5NJ_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HHY6484928.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23840..24286
                     /locus_tag="ACV5NJ_000815"
     CDS             23840..24286
                     /locus_tag="ACV5NJ_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HHY6484929.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7707 a         4351 c         5186 g         7089 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcttac gaaacccgac
     6781 ttttgtcagt agtaagagaa cgaaaacatc taatacagta ataaagacgg caatcaccaa
     6841 tggaatatca aaaagtaagt agagggcgat tgctgcacca atgacttctg cgatgtctgt
     6901 tgccataatg gctaattcag tcaagatcca taaaatgaca cccaatgtct tacttgtccg
     6961 tgctcggatt gcttgggcca aatccatttg ggtcacgatt cctaattttg ctgccatata
     7021 ctgtaaaagc atagcaatta gactagacat caaaataact gacatcaata aatattggaa
     7081 attttgtcca ccagtgatcg aagtggacca gttacctgga tccatataac caactgctac
     7141 taatgcacct ggtccagagt aagcaagaag tgttttaaaa aaactttttc cctttggtac
     7201 atccactgtt ccatttactt cagctaaaga taaaccattt gtatgttcaa taagtcgatg
     7261 cttatgaaat tgtttgttgg attgattatt caagaaaaaa ccacctttcc tatttatcta
     7321 cgattacatt ctacacctca gaaagaaaaa attaaagaaa aagattcggt atgcctaatt
     7381 ttttttcgtg gtgtgtcttg ctggttaatg aaagaaattt tgttcttctc taaaaaaacc
     7441 actatttttt gcatgaaact aaagaatacg cctttctttt ttcaataaac tttagtaaaa
     7501 taaatcattg agagcaattc ggaaatagaa aggagagatt tatatggtat caacaggtat
     7561 tgtcgtactt attgctatta ttgctttgct aatcggcgca gtaggtggct ttttcctagc
     7621 gcgtaaatat atgaaagact atttagaaaa aaatcctccc gttaatgagg acatgttgcg
     7681 atcaatgatg atgtcaatgg ggcaaaaacc atctgagaag aaaattcgcc agatgatgca
     7741 gcaaatgaag aaccaaggga aaaaataggt tctgacccaa agactttttg cgggctgatt
     7801 ttttcagtcc gttttttatt tcgtaatttg ttagactatt cagactattc agcaaaaaag
     7861 acaagcacag acaatcctat gctcttgata aagacaaagg aggagtaaaa tgtcgatttt
     7921 tagaaaatta ggttggtttt ttcgtcagga aaagaaacat tatttaattg ggattttctc
     7981 cctattcgca gtggccttgg tacagttagt tcctccaaaa gtgattggga tcatcattga
     8041 tgagatcgcc gataagaatt tatcaataaa gatcatttcc tggtggatct tattattgat
     8101 ccttgcagct ttcttacaat atctttttcg ttatatctgg cggacgaata tttggggaag
     8161 tgcagctcgg ttggagagag aacttcggca gcagctattc aaacatttta cagaaatgga
     8221 caatctcttt taccaaaaat atcggactgg agatttgatg gctcatgcaa cgaatgattt
     8281 gaatgcaatc cagaatgtag caggagctgg aatcttgacc tttgcagatt cagtgattac
     8341 gggcggaaca acgatcattg cgatgatttt gtttattgat tggcgcttaa cactgattgc
     8401 gctgattcct ctgcctttgc tagccgttac ttcacgagtg ctggggtcaa aactccacga
     8461 tgcttttcgt gattcgcaag aagctttttc tagtatcaat gataagacac aagaaagtat
     8521 cactgggatc aaagtcatta aaacatttgg acaagaaaaa gaagatctcg cggattttga
     8581 agaaaagatt gaagatgcaa ttattaaaaa caaacgagtc aattttttag actccttatt
     8641 tgatccattc attaccttga ttatcggtct gtcttatgtt gcaacaatta ttttaggtgg
     8701 aaagtatgtc ttggaaggca cgatctcaat cgggcagatg gtctcattta ttagctatat
     8761 cggtatgctt gtttggccga tgtttgcgat tggtcgattg ttcaacgtat tggaaagagg
     8821 aaatgcttct tatgatcgcg tcaatgaact tttgcatgaa aagacgcata tcgtggaaca
     8881 actgggtgca gtgcagaccc cggcaaaagg cgagctgaat gtagctattt cctcttttaa
     8941 ctatccgaaa gaagaatcac cagcattaga acaaatcaaa tttgcgatcc aagaaggaga
     9001 aacattaggt attgttggga aaactggtgc gggaaaaacg acaatcatca aattactgat
     9061 gcgtgaatac gatgattatc aagggaaaat ccaatttggc ggacgttcga tcaaagaata
     9121 ttcattgaat gctttattag gatcaatcgg ttatgtgcct caggatcact tcctgttttc
     9181 gatgaccatt cgagataata tccgtttttc taatccaaaa ctttcagaag aagaagtaga
     9241 gcatgcggct tctttagcat ttatcaacaa tgaaatcaag gaattgccaa aaggttatga
     9301 taccttagtt ggagaacgtg gagtctcttt atctggcgga cagaaacaga ggatttcgat
     9361 tgctcgagca ttgatcaaaa atcctgaatt gctgatctta gatgatgcac tttctgcagt
     9421 agatgccaaa acagaagaag ctatcttgca taatttaaaa gaaataagaa aagagaaaac
     9481 aaccatcatt gcagctcacc gtttaagcag cgtgatgcat gcaaaagaaa tcattgttgt
     9541 cgatcaaggg aaaatcattg aacgaggaac ccaccacgag ctgcttgcat taggtggctg
     9601 gtatgaacga atgtgggaaa aacagcaatt agaagcgaag atagaaggag gcgaggcctg
     9661 atggatgaaa attatcaatc ggaatggaca aaatcgattc cacttaaaga acagaagaag
     9721 atcatcaaac gactgatgaa gttcgccaaa ccttttagaa gaacgttcat cgttgctatc
     9781 ttatttgcgt ttgccctgtc tgtgatcaat gtcctattgc caaggatcat ccaaactttt
     9841 atggatgatc atctggctaa acagtcagct acgacacaag ttattttgtt ttttgctggt
     9901 gtgtacttgt ttggagtcat agtcaaaagt atcatttggt ttttccaatg gtatttgtat
     9961 tccatggctt cattgaagac ctatcagtat gttcgggtaa gattatttga aaagctgcat
    10021 acactaggta tgcgttattt tgaccagaca ccggcaggat cgaccgtttc tcgagtgacg
    10081 aatgacacag aaacgctatt tgaattttgg tatgtctttt tgatggtatt gactgggatc
    10141 tttgcagtcg tttcttcttt cgttgccatg tttcaaatca atggagaaat tgccttgtgg
    10201 actttggtgt tcttgccgat tttaggaatc gtcatctggt attaccagaa attcagttct
    10261 cgtatttacc gaaatatgcg ggaaaaatta agtcagctga acacgaaatt gaatgaatcg
    10321 atttccggca tgcgtattat ccagcagttt cgccaagaag ctcgtttagc taaagaattt
    10381 gaacaagtga acaacgagta tttagacacg cgctatgcga tgatcaaaac gaattcgatt
    10441 ctgttaagtc caatcatcaa ttttctctat gcattggcga ttgcgttggc gttgactttg
    10501 tttgggatcg atgcgttgca ttctcccgta gaagttggac tgatctatgc atttacgacc
    10561 tatgtccagg aattttttaa tccaatgtct caaatgatgg actttttgag cacctttaca
    10621 gatgggatgg ttgccggtag tcgtattttg aaaatcatgg atacagaaga atatgcaccg
    10681 caacaaaatc ctggagcaga tcaagcgatt acttcaggta agatcgagtt tcgcaatgta
    10741 agcttttctt atgatggaga gcacaatgta ttgaacaata tctcttttgt cgctaatccg
    10801 ggtgagaccg ttgcattagt ggggcacacc ggaagcggaa aaagctcgat catcaacgta
    10861 ttgatgcggt tttacgaatt ttatgaaggc caaatcttga ttgatgatca tgacattcga
    10921 gaatatccaa tggaagagtt acgtaagaaa atgggattgg tcttgcaaga tgccttcttg
    10981 ttttatggag atattgcagg aaatatccgg atgatgaatc cagctatcac agacgaacag
    11041 attcgccatg cagctgagtt tgttcaagca aacaagttca tcgatgaatt accaaaagga
    11101 tatcactcaa aagtgatcga acacggtgcc agctattcta gtggacaacg acagctgatc
    11161 acttttgcgc gaacaatcgt gacagatcct aaaatactag tattggatga agcaacagca
    11221 aatatcgata ctgaaaccga aggattgatc caagaaggat tggcaaaaat gcgacaagga
    11281 cggacaacga tagcgattgc ccatcggctg tccacgattc gggatgcaga attgatttta
    11341 gttttagaca aaggtcatat agtagagcgt gggaatcatg aaacacttct agcgcaaaat
    11401 ggtttgtatg cagatatgta caaactgcaa agcagtggtg tgtaaaaaga ggttgtgaca
    11461 gtagcatagt agcattttgc cctaagcgct agagtgaaaa taaaacagag cttcgcagat
    11521 tcttcatctg tgagcgttag tggcgtagtc acccgattta aaaaagacga cgacagctag
    11581 tcaaaagcgg ctaataacca ctaagaaagc gttgacaaaa gcgtttgctt tcgataatat
    11641 aaagaaaacg atacaacttg gatagtgatt gcctgatgca agctaaacct gatgagtctc
    11701 ccatggttcc atggacggct ttttgggttt ttctttttgt tttggataca gccctatata
    11761 tcatggaaag gagcaaaaca tgctgattga gaaagtactg aataacaacg tcgtaataac
    11821 cagggatgaa agaaatcagg aaatgatcgt gatgggaaaa ggtttggcat tcaaaaagaa
    11881 agtgggtgac ccgatttttg actcatgcat cgacaaaaaa tttcagttga caaatgacgg
    11941 gctttcacaa cagttccaag aattggcaca agcgattcct cttgagtact tagaaatttc
    12001 ttgtgaaatc attgaatata caaaagaggc tctacaagtc cagttgaatg attctgtgta
    12061 tattgcattg acggatcatt tgtatacggc tatcgaacgt cacaaaatgg gaatatttgt
    12121 accaaatgta ttgctgtatg atatcaaaca actatttcct aaagagtatg ccatcgggaa
    12181 aaaaaacagt gaagaaaatc tcggataaat accacatcaa gttatcagaa gatgaagccg
    12241 gatttatcgc attgcattta gtcaatgcac aaggcattca aagccaacaa gacaatacct
    12301 acctgttcac tataacaata caggatattc taaatatcgt ccgctattac tataagatta
    12361 catttgatga agattcagtc catttttttc gattcgtcac acacttacgt ttttttctga
    12421 cacgcgtcct agatggagaa aaatcagaag tgggagaagt ttctgaggaa ttgtttcagg
    12481 ttattcaaaa aaaatataag gatacgtttg gttgcgttga gaaaatagca gactttttag
    12541 ccgagacgtt caactaccag atgtccacgg acgaaaaaat gtatttaacg atccatactg
    12601 cgcgtttagt gcatgagaat cgttgtaaat aaataaaaaa aggaggataa attaacaaga
    12661 acgtatgaaa taagggtagt gacattcagt tggctaaacc ttccactaat aaattggagg
    12721 gaatagaaat gggaaaatac caagaattag cgagaaaaat cgtagaaaat gtcggcggga
    12781 aggaaaatat caatggtttg acacattgta tcacaaggct tcgtttcaaa ttaaaaaacg
    12841 aagaaaaagc aaatgatgag atcttgaaaa atatggacgg tattgtaacg gtaatgcgag
    12901 cgggagggca gtatcaagtt gtcatcggga atcatgtgcc agtcgtgttt gaagaggtta
    12961 taaaagcagg aaaccttact tttgatgagg ctgtttcaac agaaaaaatg agaccgtttg
    13021 atatgcttat tgatggaata tcgggctgtt tccaaccatt ccttgctatc ctagctgctg
    13081 acgggatgat tcgtggactg acagcattcc ttgtcttctt aggcgcgttt gaccgcggtt
    13141 ctggcacatt tgttatgttt gataatatcg gtgattctgt attccaattt atgcctgtta
    13201 ttatcggttt aacagctgcc cgtaaattta aagtaaatga attcgtaggg atgttgcttg
    13261 gcgcagcatt aatgaatcct agcttgtcgc tggaagcttt gagtggtgca gcagaagcac
    13321 cactaacgac catattttct ggcactattt ttgaagcacc tatttatcaa acagtattcg
    13381 gcattccctg gattgcgcgt aactacgctt cttctgtcat tcccattatc tttattgtgt
    13441 tgttagctag ccaaattcag aaaccaatta aaaagttagt acctgagatg atcgcgaatt
    13501 tcttcgttcc attcttcacc gttcttatta caatgccttt aggtttttta ctcgttggac
    13561 cagtattcac attcgcaact gatattttaa tggctggatt tgaaacactt ttagcacttt
    13621 cgccagttat ttatggagca atcgtaggct tcttctggca aattctggtg atgtttggtc
    13681 tccattgggc aatcgtacca atgggattga tgcaattttc tgtgaatggt tggcaaaata
    13741 ttatgacacc tgttgcagta gtgtcttttg gacaaactgc agcacttacc gcactctact
    13801 ttaagcttag aaatccaaaa gacaaagcaa ttgcaatacc agccattgtt tctggtattg
    13861 tggggattac agagccagct atttatgggt ttacattgcc acgtaaaaaa atatttattt
    13921 ttacctgtgt cggtggtgcg ttagggggcg cgtatagtgg gttaatgaat ttaacttctt
    13981 ggaatcaagg tggactaggc atctttacaa ttcccaacta tattagacca gatggtgatt
    14041 taacagatgt gattaatgta ttgattggga tcgccattgc gatggtggta tcctttgcgc
    14101 tgactttttt cttttggaag gaggaagcag atgaaacaga tgatagacag aaaagatcag
    14161 gtaaagaaat tgtaaaaaca ccgatacaag gacaaatcgc tccactgaat gcagcaaagg
    14221 atgcagcttt tgcacaagga acactaggtc gaggaatctt gatctaccct gaaaaaggag
    14281 aagtacgtgc cccttttgac ggaacagtca tgtccctgtt tccgacgaaa catgcaatcg
    14341 ggatggtttc tgaagcaggt ctggaattgc tgattcatgt cggattggat actgttcagt
    14401 tggaagggaa atattttgaa agcttggtgc agcaaggaga aagagtgaaa caaggcgatc
    14461 tgttgcttcg cttcgacaaa aagaaaatcg aagaggaagg atacagttta gaaacacctg
    14521 tgattgtcat caattacatg gattatttgg atattttgga aaatcaaggg gaaacggtcg
    14581 ggccagacga tgcacttatc accgcactga cctgaacggt taggtgaaag aatggactag
    14641 gcttaactat tctttttagt ccacttcttt attttcaaaa ttgaaaggct ctttcatgat
    14701 ggctgagctg atagggcgtt tatcaaattt ttgtcctaca gaaattagat ggactaaatt
    14761 ctgttgataa ttttctgcaa aaacacaact atataacacg tctagaagat aacaaatcga
    14821 tgtattgatc gtaaaattac caattttcga atacagtcgt tcgcgtgtcg taagtcgcaa
    14881 agcggctgta ctcattgaag atagcgttga atcaccgata ctggtcaaag ctaaaattgg
    14941 cacatgattt tgtttcaata tttctgcggt acgaaggatc acattgtttt cacccgtata
    15001 agaaatcaga atcccacaca tatcacttgt gcaattgtac gcttcataga acgattcacc
    15061 aaaagtggtg acaatcgtga catccatttt gatgcgcttc attttcagca tgaaatcttg
    15121 agaaatcaaa gtattcgagt tactggcgaa aatcacgatt ttttttgctt gaagcattag
    15181 ttgtttggct ttttgcagtt ggtcatgatg aatgagagaa agtgtatctt gaatcgtcat
    15241 ctgttctaaa gaagccattt tatttgcgat cgtcataata ccgtctgtgg tggaaaaagg
    15301 aagattcgca tctacgtctt gaaaattccc ttcaagatag gctgtttcag ctaaaaaagc
    15361 attacgaaaa ctgacccagc catcaaaacc catttttttt gctacacgga tgagtgtgga
    15421 cgggtggaca taacatcgct cagcaatctc ttggatggtc atttcgccaa gtgctggaga
    15481 atcgcttaag agaaactgaa tcacttcatt ttctgaagga gaaaaggaga tttctttgat
    15541 tttttcacgt aataacatgt agataccttc ttcttgtttt tttacgtaaa tcataacttt
    15601 tttgaaaatg atttgtaaac ggatttatgt tatatagctt aataaagaca ttttttgaca
    15661 agatatttac aaaaatccag aagaaaacac taacttttgt aaatgttact ggaaagaaac
    15721 gaatttctat tgtttgtttt gtttacaaaa aagataataa aagtgtaaaa agtaaaggag
    15781 tgagaacatg gcaaaaggaa tcaaaatcgc tacgattggt ggaggcagca gttacacccc
    15841 cgagttgatg gaaggattta tcaaaagata tgaggaatta ccgatacgag aaatctggct
    15901 cgtagatgta gaagaaggaa aagaaaagct tgagatcgtc agtcagatgt ctcagcgtat
    15961 gtgggatgct tctccgtatg aagtcaaaat ctatgcaact ctggatcggg aagaagcatt
    16021 gaaagatgcg gattttgtta cgactcagtt tcgtgtcggt caattagatg cacgaatcaa
    16081 agacgagcgg atccctagtt attatgggat gattgggcaa gaaacaaatg gtgctggcgg
    16141 gatattcaaa gcttttcgta ccgttccagt ggttttggca atcgttgaag acatgaagcg
    16201 tttatgtcca aatgcttggc tgattaattt cgcgaaccca agcgggatgg tgacagaagc
    16261 agttatcaga tatggcaaat gggataaagt gatcggatta tgcaatgtgc cagtaatggc
    16321 gatgatgaca gaaccaacaa tgcttggcaa gaaaaaagaa gagctgatct acaagtttgc
    16381 cggtctaaat cacttccatt ggcacaaagt agcagataat aaaggaaatg accgcacggc
    16441 agaattgatt gatcgtttat acgatgaaga caatggattg ccgaaaaata tctttgacat
    16501 cccgtttttc aaagaacaat taaaacaaat gaacatgatc ccttgtggct accaccgcta
    16561 ttactatcgt gaagaagaaa tgctggaaca tgctttagaa gaatatcaaa cgattggtac
    16621 aagagcgcag caagtgaaag aaacagaagc agagctgttt gaattataca aagatccaga
    16681 attggatcat aaacctgaac aattgcaaaa acgaggtggt gcttattatt cagatgctgc
    16741 ctgtgaaacg attgcttcga tctatggaaa taaagaaaca cagatcgtgg tatctacaaa
    16801 gaatgatgga gctgttcctg atctgccggc agattgtgta gtagaagtca ctgcctatgt
    16861 tggcgcacaa ggcgcacgaa atgtagcgtt tggttccctt cctgctgctg aaagaggctg
    16921 gttgcaagta atgaagaata tggaattgct gacgatcgaa gcagccgtga ctggtgatta
    16981 tggaacagct ttgcaagcgt ttacgatcaa tccgcttata cgatcaggaa agacagcaaa
    17041 acggatcatg gatgagttgt ttattgcaca tcagcagtat ttaccgcaat tcaaggaaac
    17101 aatcagccgg ttaaaagcag aaggaatcga agtccaagat gaactggcaa gagaattatc
    17161 aagcaataca catgtaatgg aggaagtatg atgggattca gaaaagattt tttatggggc
    17221 ggagctacag cagccaatca atgcgagggc ggttatgatg aaggcggacg tgggttagcg
    17281 aacgtagatg ttgtgccaat cggagaagac cgttttcctg tcattactgg aaaaaagaaa
    17341 atgtttgaat ttgatgaaaa tcatttttat ccagcaaaaa ttgcgatcga tatgtatcat
    17401 cattataagg aagatattgc tttatttgca gaaatggggt tcaagactta tcggatgtcc
    17461 attgcttgga gtcgtatttt tccaatgggc gatgaaaaag agccaaatga atctgggttg
    17521 aaattttatg aggatgtctt taaagaatgt caaaagtacg ggatcgagcc gcttgtgacg
    17581 attacacatt ttgattgtcc gatgcatttg atcaaagaat atggtgcgtg gcgttccaga
    17641 gaactcgttg gtttttatga aaatctgtgc cgagtcatct tcaatcgcta taaaggactt
    17701 gtcaaatatt ggttgacatt caatgagatc aatatgatcc ttcatgcacc attcatgggc
    17761 gcagggctgt attttgaaga aggagaaaat gaagaacaaa tcaaatatca agcggcacat
    17821 catgagttgg ttgcaagtgc catcgcaacg aagatcgctc atgaagtaga tccggagaac
    17881 aaagtaggct gtatgctggc agcaggagca tattatccat acacttgcaa tcctgaagat
    17941 gtttgggctt ctagaaagtc agatcgagaa aattatttct tcattgatgt ccagtcacga
    18001 ggagaatatc ccgcttatgc attgaaagaa atggaacgaa atggcattca gatcaaaatg
    18061 gaacctgggg atgccgcatt gctgaaagaa catactgttg attttatctc cttttcttat
    18121 tattcttctc gagtttcgac tactgacaac gaactcttgg aacagactgc aggaaatatc
    18181 tttgcttctg tcaaaaatcc atatctggaa gcaagtgaat ggggttggca aattgatcca
    18241 ttaggcttga gaataacgat gaatgatatt tatgatcgtt atcaaaaacc actatttatt
    18301 gtagaaaacg gcttaggtgc agtcgatacg cccgatgaga atggttatgt ggaagatgat
    18361 tatcgaatcg actatctggc agcacatatc caggcaatga aagatgcagt agaattagat
    18421 ggagtcgatc tattaggcta tacgacttgg ggatgtattg atttagtatc agctggtaca
    18481 ggtgagatga agaaaaggta cggattcatt tatgtcgatc gtgacaacga aggaaatggc
    18541 acattagccc gttcgaagaa gaaatcattc aactggtata aaaaagtaat tgattcaaat
    18601 ggagaaaatc tttcgaacga ttgatcaatg atttataaaa gtctattgtt tttcagcgga
    18661 tttttcgagt ataataaagc tattcgagaa acgcaagcag aacaatagac tttttttcgc
    18721 attggctgta aagtatgctt acttgattaa gtaaaggaga atctacatat gtatgatgta
    18781 atcgttattg gcgcagggcc tgctgggatg actgctgctt tatatgcttc acgttcaaac
    18841 ttatccgtat tgatgatcga acaaggcgct cctggcggcc aaatgaacaa tacggcagaa
    18901 atcgaaaact atcctggttt cgattcaatc atgggtcctg atctagctta taagatgtat
    18961 gaaggtgttt caagatttgg gacagaaaat gcttatggaa tcgtgcaaga tatcaaggat
    19021 cacggcagcc acaaagaagt tatttgtgaa gataaaactt acgaaggaaa aacagtcatt
    19081 atcgcaacag gctgtatcca ccgtaaatta ggcgtacctg gcgaggaaga atttgctgga
    19141 cgtggcgttt cttactgtgc agtttgtgat ggtgcatttt tccgtaacaa acgtctagta
    19201 gtgatcggag gaggagactc agcagtagaa gaagccatct atttgacaca atttgcttct
    19261 gaagtagtca tcgtccaccg tagagatgaa ttacgtgcac aaaagatcat ccaagaccgt
    19321 gcatttgcga atgaaaaaat ttcctttatg tgggatacag tcgttgacga aatcgttgga
    19381 aatgaaatgg tagtgacagg tgtgaaagca cgtaatgtca aaactggcaa tgagtttgat
    19441 ttggcagcag acggagtatt catctatgta ggattggatc cattaactgc accatttaga
    19501 gaagctgggt tgacgaacga agccggctgg attccaacag acaacgaaat gaagacgaat
    19561 ataccaggcg tttttgccat cggagatgtt cgtgaaaaag atttgcgaca aatcactact
    19621 gctgttggtg atggtgggat tgctggacaa caagtgtata aatacttgga atctttagaa
    19681 gaaacaactg cataagttta aaaagagtga aagaacggtt tcaattgttc ttttactctt
    19741 ttttttgcgt gaaatctact gaatccgatt tatgcgaata cttgtaaccg tgcttgataa
    19801 aagaatcttt tcttgattta agcgtttttt ggtcctcata ctttcatatc taataagtga
    19861 atgctatact tattaagaac catatttcag aaagaggtgt tattttcatg tcttgggaac
    19921 aagtgtatga acaatgggta catgaagaaa atttagaaga aaacttaaaa aaacaattaa
    19981 atgatctggg tgaagatcct gaaaaacttg aagatgcttt ttatgcaccg ttggaattcg
    20041 gtactgcagg catgcgggga atcttaggac ctgggatcaa tcgaatgaac atctatacgg
    20101 taagacaagc aacagaagga cttgcacgtt tcatggatac acaaggtgaa gaaacaaaac
    20161 gccgaggtgt cgctatcgct tatgactcac gccatcaatc accagaattt gccatggaag
    20221 cagccaaaac attagcacat cacaatatcc cttcttatgt attcgaaagt ttacgtccaa
    20281 caccagagct ttcttttgca gtccgtcatt tgaagacgtt tgcgggaatc atggtaactg
    20341 catcccacaa tccagcagct tacaacggat ataaagtcta cggagaagat gggggacaaa
    20401 tgcctcctgc agacgcagat gcattgacga aatatgtccg tgaagtcagc aatccgttaa
    20461 aagttgaagt attatctgac gacgaagctg aacatagcgg attgatcacg atcatcggag
    20521 aagaagtgga tgccgcttat ctagaagaaa tcaaagcagt aacgattgat catgaattgg
    20581 tggaaacaat gggtaaagat ttgaagcttg tgtacacacc acttcacgga acaggaaaaa
    20641 tgttgggcga acgtgcgttg aaacaagctg gattcgaaca attcgtacta gttccagaac
    20701 aagctgtcgc agacccggat ttcactacag tcaaatcacc gaatccagaa gaacactctg
    20761 ctttcgagta tgcgatgcga cttggcgaga aagaaggcgc tgacttactg attgcaacgg
    20821 atcctgatgc agaccgttta ggcgcagccg ttcgtttgcc aaatggctcc tatgaagtat
    20881 tgacaggaaa ccaaatcgga gccctgtttg ctcaatatat tttggaagca caccaatcgc
    20941 gaggcacttt acctgaaaat gcagcggtat tgaaatcaat cgtttctagc gaattgccta
    21001 cagcaattgc ggaacactat ggggctacca tgttcaatgt attgacagga ttcaaattca
    21061 ttgctgaaaa aattcaacaa tttgaagaag atcattccca tacttttatg ttcggattcg
    21121 aagaaagtta cggttattta gttaaacctt tcgttcgcga caaagacgcg attcaagcat
    21181 tgctcttatt agcagaagta gcagcttact ataaaaaaca aggcaaaaca ttgtatgacg
    21241 gcttgcaaac gatttttgaa acatatggct actacgcaga aaaaacgatt tctgtgacaa
    21301 tgagcggaca agaaggctct gcaaaaatct ctgctttgat ggaaaaattc cgtgaagaag
    21361 caccgacaga atttgccgga gcaaaagtca tccaaacaga agacttcaaa gagttgacga
    21421 gaacagaccg tgaaggaaac gtagaaaaac taacgacacc gccatctgac gtattgaagt
    21481 atacgctgga agaaaatggc tggatcgcta tccgtccttc aggaactgag ccgaaaatca
    21541 aattttatat cggtgtaaaa ggaaactctt cagaagacgc agctgaaaaa gtagccgctt
    21601 atgaagcgat tatcaaaaaa atgacagaag aataaaaaaa cttgagactg atggaaaaag
    21661 tggtaaactt tagaagtcag tcttttttca agttggaggt gaatggattg ccagacacgc
    21721 aaaaaacaaa agtagagaaa gtcagccaac tattcaaaat gttgagtgac cccactcgtc
    21781 tcaaaattct gctgtatctg aaagacgggg aacaaaatgt gacagcaatc acccaagcag
    21841 tagaaatgga acaatctgct gtatcgcatc aactgcgttt attaagagaa aaccatgtcg
    21901 tcaagtccca tcgtgaggga aaagcgattt tgtatagtct tgatgattct cacgtactgg
    21961 atattttgaa ccagacgctc aaacatgtgg aacatcaata acgataggtg caaatagaga
    22021 caaactggac tgtatcaacg aatcttcttc gctgacacag tccagttttt ttaagaaaaa
    22081 gacaagaaaa actgttagtc tgtttcttcg gtggtttcag ttccataata actatgatct
    22141 tctaggtcta aagcattcaa aataagtgaa gctgtttctt cgatagataa agaagcgaca
    22201 ttgattacta cgcagcccaa tttttcataa agatcattcg caaaatcgag ttcagctcgt
    22261 attttttcga tatcggaata agcagtatca ggatttaatc cgtaagagcg catccgttct
    22321 ttacggatat tgtttaaaat atctgggtta ttcgtcagtc ccacgatttt tttaggatcg
    22381 atctcccaaa gctgtttggg aatatgtgct tgtgggatca aaggtagatt ggcaaccttt
    22441 aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg aagttctgga tacgcctagc
    22501 aaaacgacat ctgcttctag aaacccacga ggatctttcc cgtcatcgta ttttacagca
    22561 aattccattg ctttgatccg tttgaaatag ttctcattca ataaatgaag ggcgccaggt
    22621 tgccgaatgg gcttgatacc agttaagcgc tcgatttctg atatcggcgg agtcatcaca
    22681 tcaaaactaa ataattgatg ttcttcacaa aaagaacgcg tcatctcgat caattcttga
    22741 ttgatcaatg tgtgtaggac aagggctttt tcttttagtg catcttgcag tgcctgcatc
    22801 aatgttttct catctgtcac aaatgttctg cggaataaat tgaactcaac ggtaggatat
    22861 tgggccatag tggcttgcgc taattttgtc gcagtttctc cagcagagtc agagatgaca
    22921 aaaaaagtaa tgatttctgt agaatcggaa gacatggata aaacctcact tgcatttttt
    22981 ttatgtttat tataccatac tctagaatgt gtacttcgta atcattttga tttggaggaa
    23041 taagtcatga aaaaacagac attgattgaa caatcactag aaacattgaa aaataacggt
    23101 tttaaatata ctaaaaaaag agaagcattg ttaacttatt tagtgaagcg caatcgctac
    23161 gtatccgcta aagaagtttt tgactatatg aatcaggaat tcaaaggagt aagttacgat
    23221 acgatttatc gaaatctcca tgattttgca gaaatcgatt tactggaaga aaccgattta
    23281 aatggagaga tgaagtttcg ttttcattgc tgtcaagggg aaatcggcca tcaccatcac
    23341 tttatctgta cggtgtgcgg aacaacgaaa gaaatccata tgtgtccaat gaattatttc
    23401 gaagaacagc tcggtggatg tacgatcgaa gggcatagat tcgagatttt cggaagatgt
    23461 gaaaattgtc aataaggatt taaataaaat aacatttgat aattttaaaa aaaagcaaga
    23521 tttccttgac ggtcaaaaca gcattagata taatatttac aggacagtct tgttttttga
    23581 gatatatttc aataacaatt acggttactt tttaagctcg gagggaggga attaacatgt
    23641 caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc tcttcgtcgc ttcaaacgtt
    23701 ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg tgaattctac gaaaaaccaa
    23761 gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg caaaaaattc tagttctgtg
    23821 atgaattgga gttggtagtg tgtcactact aagtagatta aacgatgaca tgaaaacagc
    23881 gatgaaagcg aaagataagg aatctttaca agtcatccga atgatcaagt catctataca
    23941 aaacgagcag attaaagaag gtcacgactt gacagaagaa gaagaattaa cggtgttgtc
    24001 tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt gaagaagcag gacgtgacga
    24061 tttagctgaa aaagtaaaaa gtgaaattgt gatagtcgaa aaatacatgc cggaacaact
    24121 ttccgacgaa gaaattcgac aacttgttca agaagcaatc acacaaactg gtgcttcatc
    24181 aatgaaagaa tttggcaaag tcatgggtgc gatcatgcct aaagtcaaag gaaaagcaga
    24241 tggaaaccaa gtaaatgcca ttgtgaaaga actattacaa tcttaatttt atcatttaaa
    24301 ggctcttcgt caatgagtgt tggtgaagaa att
//
LOCUS       DBKOIJ010000026        24321 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000026 DBKOIJ010000000
VERSION     DBKOIJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.026"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(110..844)
                     /locus_tag="ACV5NJ_000816"
     CDS             complement(110..844)
                     /locus_tag="ACV5NJ_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HHY6484930.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1171..1581
                     /locus_tag="ACV5NJ_000817"
     CDS             1171..1581
                     /locus_tag="ACV5NJ_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HHY6484931.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1574..2275
                     /locus_tag="ACV5NJ_000818"
     CDS             1574..2275
                     /locus_tag="ACV5NJ_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HHY6484932.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2275..3861
                     /locus_tag="ACV5NJ_000819"
     CDS             2275..3861
                     /locus_tag="ACV5NJ_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6484933.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3880..4101
                     /locus_tag="ACV5NJ_000820"
     CDS             3880..4101
                     /locus_tag="ACV5NJ_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HHY6484934.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4098..4859
                     /locus_tag="ACV5NJ_000821"
     CDS             4098..4859
                     /locus_tag="ACV5NJ_000821"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HHY6484935.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5239..5748
                     /locus_tag="ACV5NJ_000822"
     CDS             5239..5748
                     /locus_tag="ACV5NJ_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HHY6484936.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5823..6362)
                     /locus_tag="ACV5NJ_000823"
     CDS             complement(5823..6362)
                     /locus_tag="ACV5NJ_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HHY6484937.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6502..7113)
                     /gene="rpsD"
                     /locus_tag="ACV5NJ_000824"
     CDS             complement(6502..7113)
                     /gene="rpsD"
                     /locus_tag="ACV5NJ_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HHY6484938.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7375..8226
                     /locus_tag="ACV5NJ_000825"
     CDS             7375..8226
                     /locus_tag="ACV5NJ_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HHY6484939.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8254..8889
                     /locus_tag="ACV5NJ_000826"
     CDS             8254..8889
                     /locus_tag="ACV5NJ_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HHY6484940.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8909..9682
                     /locus_tag="ACV5NJ_000827"
     CDS             8909..9682
                     /locus_tag="ACV5NJ_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHY6484941.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10090..10887
                     /locus_tag="ACV5NJ_000828"
     CDS             10090..10887
                     /locus_tag="ACV5NJ_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HHY6484942.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10865..11278
                     /locus_tag="ACV5NJ_000829"
     CDS             10865..11278
                     /locus_tag="ACV5NJ_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HHY6484943.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11262..13907
                     /gene="mutS"
                     /locus_tag="ACV5NJ_000830"
     CDS             11262..13907
                     /gene="mutS"
                     /locus_tag="ACV5NJ_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HHY6484944.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13925..16033
                     /gene="mutL"
                     /locus_tag="ACV5NJ_000831"
     CDS             13925..16033
                     /gene="mutL"
                     /locus_tag="ACV5NJ_000831"
                     /inference="COORDINATES: protein motif:HMM:NF000950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HHY6484945.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16055..16615
                     /locus_tag="ACV5NJ_000832"
     CDS             16055..16615
                     /locus_tag="ACV5NJ_000832"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HHY6484946.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16700..18688)
                     /gene="treP"
                     /locus_tag="ACV5NJ_000833"
     CDS             complement(16700..18688)
                     /gene="treP"
                     /locus_tag="ACV5NJ_000833"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HHY6484947.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18911..19624
                     /gene="treR"
                     /locus_tag="ACV5NJ_000834"
     CDS             18911..19624
                     /gene="treR"
                     /locus_tag="ACV5NJ_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HHY6484948.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19701..20147
                     /gene="msrB"
                     /locus_tag="ACV5NJ_000835"
     CDS             19701..20147
                     /gene="msrB"
                     /locus_tag="ACV5NJ_000835"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HHY6484949.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20317..20919
                     /gene="ruvA"
                     /locus_tag="ACV5NJ_000836"
     CDS             20317..20919
                     /gene="ruvA"
                     /locus_tag="ACV5NJ_000836"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HHY6484950.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20932..21933
                     /gene="ruvB"
                     /locus_tag="ACV5NJ_000837"
     CDS             20932..21933
                     /gene="ruvB"
                     /locus_tag="ACV5NJ_000837"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HHY6484951.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21962..22540
                     /locus_tag="ACV5NJ_000838"
     CDS             21962..22540
                     /locus_tag="ACV5NJ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6484952.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22592..23074
                     /locus_tag="ACV5NJ_000839"
     CDS             22592..23074
                     /locus_tag="ACV5NJ_000839"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HHY6484953.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23191..23565
                     /locus_tag="ACV5NJ_000840"
     CDS             23191..23565
                     /locus_tag="ACV5NJ_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484954.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23940..>24321)
                     /locus_tag="ACV5NJ_000841"
     CDS             complement(23940..>24321)
                     /locus_tag="ACV5NJ_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6484955.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7923 a         4359 c         5206 g         6833 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaaataaa
       61 aaaacttggt acgcaatcgc tgtaccaagt ttttttattt agcttttgat taagccataa
      121 ataataattt atataagaat actgctacga caccagcaca gataggtgct acaactggta
      181 cccatgcata tccccattga gaagaacctt tatgtttcaa tggaagaagt gcgtgtacaa
      241 gacgtggagc aaagtcgcgg gctgggttta gtccaggacc tgtaggacca ccaagtgaag
      301 caactaatgt ccatacaagg aaaccaagtg ctaagtgagc tgtaccaaga ttatcttgga
      361 agaatggtgc ttttgtaata cctagggcac caaagaataa aacgaatgaa ccaaagaatt
      421 cattgacgaa accattgaat ttagaaccaa cgctgttgat tgttgagaat gtacctaaca
      481 catggtttac gtcttctgtt tggtcgtagt aaggtttgta acaagctact acgatcaatt
      541 gtccaatcgc tgcaccaatc aattgtgcaa tgatataagg aacaacttca gcccaaggga
      601 atagaccatt agatgctaaa gcaattgtaa atgctgggtt gatgtggtta ccgctgattc
      661 caccaaaaat cattgctggc atcataaccc cgaatccata cccgacagca atcagcatcc
      721 agtcactgcc atatcctttt gtacctttta aatctacgtt tgctacagca ccgttaccta
      781 gaacgattag taatgctgta ccaatcattt ccgccgcaag acgggtcatt agtgagtaat
      841 ccataaatac ctccaaaagt tataaaaatg caaaaaaaaa agaaacatca acacgaaaag
      901 catcggtgca gtgtttcgtg tcggaaggat ttctgcagtc ttaacaaatg tgaataaaaa
      961 aatgaattcc attacttata agaaaccaga ccaacagtaa aactccaact ctttttaaaa
     1021 gttctccaca aatcaccaag cttaactata aatataccaa aaaaaaaacg ttttttttct
     1081 caaaaattgt atcttagaaa cagcatgttt tgaaactttc agaaaaggtt tgttagtaaa
     1141 atgaaagcta tacgtaagga gtgttgtttg atgaaatttt ttcaagagtt tttcattatt
     1201 attttctttt ctgtattagg agaaggcata cggatttttg ctcatttacc tattcctgga
     1261 agtatcttag gtattctttt tttattttta gcatttgaaa tgcatcttgt tgatcctgaa
     1321 aaaatcggag caactggtga ttttttattg aataatctag ccattttatt tgttcctgct
     1381 ggtgtaggtt tattggaata ttttgatgat attgcttcga tatggcctgt tttactggga
     1441 gcagtcgtcg tgtgttccat cgtcacgatg gttgcagcgg gaaaaacggc agaaggtgta
     1501 gaagctttgc tgggctatgt ccgtaagaaa aaagcagaaa gggtatctgt aaacgaaatg
     1561 gaggagaatc aaattgactg agttttttgt gaatccatta tttggcttaa gtttgacttt
     1621 aggagtgtac gtattatctg acaaattttt gcggaaatat aaactgcctg ttttgaatcc
     1681 tctcgtatta tcgattgttg ttattatctt atttttgcat ttttctggga tcagctataa
     1741 agactataat atcgggggca attttttgac tatgctgatt actcctgcga cagtggcact
     1801 tgccatccct ctttataaaa acttccgttt actgaaagag aatttttatc ctgtaatggc
     1861 tgcgatttta gcaggaatag tcgcaaattg cttggttagt gtagggttcg gttacttctt
     1921 ctctcttcat aaagagatgg tgatctcatt actacctaaa tctgtaacga cggctatttc
     1981 tgtcgattta tctcatacga tgggcggggt gaatgccgtg acacttgcta ttgttgtctc
     2041 tacaggtatt tttggttcgc tgatcgccac tcatattttc cgcctattca agatcgaaag
     2101 tccagttgct cgcggtgtgg cgttaggaag tacttctcat gctattggta cagcaaaagc
     2161 aatcgagatt ggtgaaatag aaggaatcat ttccggactt gcgatatgcg tcaacggaat
     2221 attgaccgtt ttattgctac cgttattctt tcagcctttt gctggcttgt tttgatgcga
     2281 aaaccactat tttatgctac aataaccaaa acgttcgagc ataaaaaagg agggaaagac
     2341 gtgtatttgt cttccgagta tcttaaacaa tttgataaag atctatatta taagattctc
     2401 ctcctagaaa gttttgagga tcaagcttgg cataccgctg cacaattagc gcaagttgtt
     2461 cagctggatg cccgcagcgt gtcgaaatac ctgaacgagc tgtctaaaaa ctatcagcag
     2521 tttagcggta agacacaccc tttgtttaca aaaaatcacc gttctggtta taacttttat
     2581 gatacgctcg attcaattga acatgagcgt tttttgatct atctcgtgca aagtacgctg
     2641 aaattccagt tgctgcacga catttttttt gaagaattcc atacaatgta ccaatttgcg
     2701 caaaagcatt acattagtga atctactgct catcgaaaaa tcaatgaatg gaaacagcaa
     2761 ctgcaaactt acggaattcg gctgcaacga ggtacatata tcgctcaagg cgaagaagaa
     2821 atcattcgac tttacttgca tatgacattc tggcagttgt ttcgagggaa aatctggcca
     2881 tttgaaacga tttctcaaat ggacgtaaag aatatggcag aacatatcat ggcttttttt
     2941 aatgttcgtc tgaacgagat caaaaaaaga cgattggaat atatgctagg tgcctttttt
     3001 ttaagaaaat cacagaagca ttatgttgta ttgaatgaga aaaaaagacg gctgatttct
     3061 gacaatctgt tgttccagcg attttgccag gtcatggagc cggtatttcc taattatttt
     3121 caagtagaag acgagttagg cgcattattt cttgtactga tgactcgtga agaatattat
     3181 agtgatccta agatacggaa gaaaattttt gattttcatc aacaagcaaa aacaccgccc
     3241 tttacagccc tttcagaagc aaaagccgct ctttctcttt atcaggaaga gcaggggctg
     3301 ccagcagaaa atctaacctt cgaggcagaa aactatttat tcagcagtca ttttttcgcc
     3361 tatctttttc ctaatgcgaa agaaacgatt gatggcaata gtagtgattt tattaatcat
     3421 ttagttattg aaaacaagga attgaaacaa tggctcgtac atttttttga aagtagacac
     3481 aaacatccca atcatctggc atttaaaaat catgcgtttt tgatggggcg gtatctgact
     3541 gtgtttaaaa cattaggagc ctttactcca cagcttccaa agatcacgat ccttttaatg
     3601 acagatttcc cattatttga agagcaactg ttagaggagg gattacggac atttttccgt
     3661 aatgagtatc agctgatttt tctgccaaca gattatcggg ggagagaagt tgatcttctg
     3721 atttcgacaa gtaaagtcca ccgcaaacca tgggcggatc ttgactattt tattgtgact
     3781 gaagaactaa aattaattga ttacatccaa ctatctcaaa aatttgaaat gattcaaaaa
     3841 caaaaactat taaaacaata acaaagaagg agaaaaaaga tgacatttga agaaatttta
     3901 ccagaattga aaaaaggtgc aaagattatt cgtaaaggat ggagtggatt tgaactttat
     3961 gttgttctcg tatccgatga tgaatttgat ggctgtccgg tgacgccgta cttattgatc
     4021 aaaacaagtg acgaaggttt ttccagcttc gctcctacag tttgtgatat tttagcagat
     4081 gattgggcga tcgtagaatg aaaaaccagt atccagatct agctaaacag gttgtattta
     4141 tcacaggagc tgcttctgga attggtgctg cgcaagcccg agcgtttctt gaacaagatg
     4201 cttttgtatt tggattagat caaaagttcg gtaaaatgga agaaatttct ttgcgatttc
     4261 ctgaaacttt tgcctacgca ctaggtgatg ttcgacaaat gaaagaactc caacaagcgg
     4321 tatcgaaatg ccaaaatcat tttggtgaag ttacgatttt actgaataca gcggggatat
     4381 tggatgccta caaacccttg atggaaactg acgaatcgct gtgggatctg atttatgaga
     4441 cgaatgtgaa aagcatgtat cagctgacaa aaatcatcct tcctgatatg atccgccaaa
     4501 aaagcggaac gatcatcaat atggcttcga ttgctggctt gattgctggt ggaggtggga
     4561 tcgcttatac aagcgcgaaa catgcgattg tcggatttac caaacaactc gctctggatt
     4621 acgcaggaca gggtatccga gtaaaaggaa ttgctccagg agccatccaa acaccaatga
     4681 atgcggctga ttttgaaggg aaaggtgaga tggcagagtg ggtggcaaat gaaacaccag
     4741 tcaagcgatg ggcacagccg gaagaagtag ctgagctgac gttatttctt gcctctcctc
     4801 aagcaagcta catacaagga acgatcatcc caatcgacgg cggatggttg ttaaaataac
     4861 cttgtgtttt tttcaagctt ctgtatgata aaactaaaga gagaaacaac ggttgatgat
     4921 aaaaaaagtc gatgactgtt cccgacattc gatgccgaac agccagttca aaaaattctt
     4981 tttaataatc gttaatattc gaaactaagc ggcttttcac gacctcttga tataaacgat
     5041 attcaaaagc ttgcaactgc ggtaacaagt ccagccaaat gaaaaatata aggagctatt
     5101 tttatttgac cattcttatt acttgttgca gggcagcttt ttcactacct tctgcgacac
     5161 ttcagttgga attttcttcc catcctttgg tcgcggtcat taccaagata aaattatctt
     5221 gcaaaggaga aatagaacat ggaaaaaaat aatacccgtt cagccaaacg ctggacaacg
     5281 acagatgtga ctcggatggc gctagtcact ggtttatatg tggcagtgac gctggttttg
     5341 tctgtcttta gctttggagt gattcagatt cgtctctcgg agatgttcaa ctatctgtcc
     5401 ttgtataaca aaagatacat cgtcgcggta acagcgggag tagctttggc taatattgct
     5461 tctcctcttg gactgataga tgttgcagta ggaagcgtca gcacgctgct tgtattgctt
     5521 atcaactata aaatcactag tcgcatcaaa aacatgaaaa tcaaaatggc cgtgacagca
     5581 tgcgtatttg ctttttccat gtttacagta gctggtcagc taacgatttt atatcaacta
     5641 ccttttttct tgaattggtg ggtgattgct ttaggagaat tggcatcaat gattgtcggt
     5701 gggataatta tttattggat cggtaaaaaa gtggatttgt caaaataaag atctgtttaa
     5761 tttaaaatag ggcagcatgg aagagactga atgttgagtc ttcttccatg ctgctttttt
     5821 gattaatttg tatgaagaaa tcgatttagc cgttttttca tcaagatacc gatataagct
     5881 gctccagttg ccttgattgc ttccggcagt aaaaacggca gtagtccact ggcaaatgca
     5941 gttgtaaaag taagatcacc acttattttc agccagatcg ttccgcagat caatgcagca
     6001 agagagccca ttagattggc aaagattgca tataagtaat gagcatccgt tctttcgatc
     6061 atccaaccag ttatccacgt ttgtaagaga aatccaatca aatatccgcc agttggccca
     6121 aataaaacag ctatacctcc agtcatccct gaaaatacag gaagaccaat caagccaagt
     6181 aaaagataaa ctccaacaga tatcaatcct agttttcttc cgagaaaagt tgctgttaat
     6241 ccaatagcaa atgtttgccc tgtcaaagga accaaaccaa aaggaatggt catctgagaa
     6301 aggacagaaa taatcgcggc aaattccgca gcaagtatca attctttcaa tgttcggttc
     6361 ataacgccct cttttcgtta acttaatatt tttatttggt taacgaaatt gtaaaacaaa
     6421 gtgattactt tgtaaataga aaatataaaa aaagaccagc tatccaatag atagcggtct
     6481 ttttcaagaa agcttttgaa attataagct ttggttgtag tattcaacga caagagcttc
     6541 gtcgatttct ggatatagtt cgtcacgttc tggtaaacga gtcaatgaac cttccaattt
     6601 ttcagcgtca aagcttacga atgctgggcg tccaacagtt gcttctacag cttcttttac
     6661 tgttacgatg ttttgtgatt tttcacgaac tgagatcact tgacccactt ctacgtggta
     6721 tgaagggata tcaacgcgtt tgccatcaac taaaatgtga ccatggttaa ctaattgacg
     6781 tgcttgacga cgagtcgttg caagtcctaa acggtaaaca acattgtcta aacgttgttc
     6841 tagtaagatc atgaagttaa caccatgttt accttctttg attttgctag cttttacgaa
     6901 caagttacgg aattgacgtt cgttcattcc gtacatgtga cgaagttttt gtttttcagt
     6961 taattgcatg ccgtattctg attgtttgcc acggctatta ggtccatgtt gacctggtgc
     7021 gtaagggcga cgtgctaatt cttttcctgt gcctgacaat gagatgccta agcgacgaga
     7081 aattttccat gatggtcctg tataacgaga cattaaaatt tcctccaatg aatataagtt
     7141 tttggagtaa aataatccgt tgaaaaattc ataatcatat agtttgttct tcaatcttca
     7201 cctttgcagc cgcggttaca caatcgaacc cgagacgggg caacaaacta gggatgagtt
     7261 attatttttc tgctgcatta ttttacacaa attacagtat atgatagatc atccacgaat
     7321 gtcaagataa ttagtacata aaaacgcttt acaaacctag gctgtcttta taaaatgaga
     7381 gggtatgtgc aaatggaaaa aagcgaagga agtttaagga gaaaatcaat gaatccggta
     7441 tcaccattat ttgagaaaat cgatgcttca attgaaaaga aggcgaatct ttttgaaaac
     7501 agcttttcac gttacgctgt tcgagcggtt ctcgcctgtt tgtttttgac tttaggaacg
     7561 gctgtggcat ttgcgatcgc gatgaagggg gaagatatcg cgcatggtct aggaaagatt
     7621 ttatacgcat ttatgttcag ctggggtctg gtcatgattc tatacatgaa cgccgaatta
     7681 ggaacatcga atatgcttta tatgaccgtg ggtgtttttc ggcagagact tgctttaagt
     7741 aaggcagcca agattctatt tgcctgtatt tttttcaata tgattggcgg tatattatgt
     7801 ggatttctaa tttctatgac tacgccattt caagatttaa cgttggacaa ctttatgtta
     7861 gaatcaatag cagtaaaact agcaaaacct tcagcaacga ttttggtgga agggatgttt
     7921 gcgaatatcg ttgtcaatac cgctgtactg atcagcatgc ggatgaaaga ggatgtcgga
     7981 aaagtgatca cagtcgtatt catcattttc atttttgctt ttttaggata tgaacacgtg
     8041 atagcgaatt ttcctgcctt tacgcttact tactttgctt ctcacggaca gatggatgga
     8101 atgacagtca gcaatgtgtt acataatttg ttttttgctt tggcaggaaa ttatattggt
     8161 ggcggtttag taatgggact gggatatgct tggctgaatc agtcaaaatc atcctatgtg
     8221 gactaaaaag tcgttcatta aaggggaagt gccatggaag ttttagtttt tccaaaaaat
     8281 gaggaaaaag cgttacaaat ggaaaagtac atgaaaaatc ttttttcctt tgctggagta
     8341 cctaagccgg aacgcgctct tttacaacaa ccgttactgg atgagagcaa aaatttatct
     8401 atttctgaag ttttagcttt tgttcattat tattatgaac aaatcgaaag agagtatcag
     8461 tatgtagcga tcgatttagt ccagaaaaac atcaaacggc ttacatttga agacttagtc
     8521 gtattgttgc cactagttca gaaaaaagaa tggtgggaca gcatcgactc ttggagaaaa
     8581 gtatacagca catggtgtaa agcctaccca gagcagcgtg aagaagtctt tcaattgttt
     8641 gaaaaaaagg aagatttttg gctacgtcga atagcaatca cgttgcaatt aggttataaa
     8701 cagcaaacag acacaggact attgatccga gcaatagacg aagatcgtca aacaaaggaa
     8761 ttctttatcc aaaaagcaat tggttgggca ttacgcgatt atagtaagac cgatgccgat
     8821 tgggtaaaaa gacagctgac aacagatttg agtaagttag ctgttcgaga ggcaagtaag
     8881 tatttataag aaagcgagac gacaagagat gagacgcaaa ttatttgctt ttgatattga
     8941 tggaacgttg ttagattcaa acaaacaagc tttagatagc acaagagaag cattggaaaa
     9001 attacgcaaa gccggtcatc tggtcacgat tgctacaggg cggagccgat ttcatgcgca
     9061 agagatcatt cgcgatcttg attttacgaa ctacatttta tgtaatggag cggccggttt
     9121 tttagaccat gaacaagtat ataagaattt actagatcgc gaccagttgg atgcgttcgt
     9181 tcaagaagcg catcatgagc agatcgatac agcttttgtc agcttagaca atatcaagcg
     9241 ttcttcttcc aatgatgtaa cggtgatgga agaagcgatg cattctttcg gtgctgtctt
     9301 gcctgaatta gatcagtttt ttgcagagga acaagaaata taccaagcgt tagcattttt
     9361 cgatcaaaca tatgatggga agttctcttc atatgataaa attcgatttg tccgctggca
     9421 tgagaacagt gtagatgtcg tacctaaaaa tggttctaaa gcagcgacaa ttctgcactt
     9481 agcagaacaa gtaggcatcc ctcatgaaga catcatcagc tttggcgatg gacaaaacga
     9541 ccgtgaaatg ttgcgcatgt caggaattgg gatagctatg gggaatgctg tgccagaagt
     9601 acaagcagaa gctaagatgg tcactgatac aaacgatcag gatggaattt ggaaggcatt
     9661 gaaagagtta agcgtgattt aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa
     9721 gcccagccaa acaaaaatag ctcctcctat ttttcgtttt tctgggctta ttaccgcaga
     9781 tgcaagcttt cgaacaccgt ttaaggaaga ggtcgtgaca gaagtggtca gtttcaagaa
     9841 ataagaaggg atttacgaaa attgcttctc aaatttttgt aaaattacgg cttatttctg
     9901 aagaagtaga tgaaacaacg tttattcggg aaatgggact gtgacaagat gtttgtcata
     9961 gtcccatttt tttattttat ttttcaaaac taagaaatac caggaaagag ttccttttgt
    10021 ttgacctttt ctttcttatt gaaaacaact ataatataga gtgatgttaa aagagaagag
    10081 gaatttagtg tgcgggtatt gtttattggt gatgtagtag gttcaatggg acgagagatg
    10141 attactgaat atcttcctag attgaagaag aagtaccgtc cgcaagtaac gattgtaaat
    10201 ggtgagaatg ccgcttctgg acgtggaatc acagaaaaaa tctataaaaa atttttgcaa
    10261 gacggcgtag atgtcgtgac gatggggaat catacgtggg acaaccgcgg aatctttgag
    10321 tttgtaaatg aagctaaaaa gatggttcgt ccggcaaatt ttccggaagg gacgcctgga
    10381 caagggatgg tctttgtcaa agtgaaccaa gtcgaactgg cagtgatcaa catgcaggct
    10441 cgttctttta tggtggactt ggatgatcct tttcgtaaaa tgaatgagct ggtagaagaa
    10501 gccagaaaac gtacgccgat catttttgta gattttcatg gagaaacaac aagtgaaaaa
    10561 caagcaatgg gctggttttt agatgggaaa gtttcagcag tggtcggtac acatacacac
    10621 gttcagacaa atgatgcgcg aattttgcca agaggaacag cgtatctgac agacgttggg
    10681 atgacaggtc cctatgatgg tattttgggt atgcgcagag aacctgtaat cgaaaagttt
    10741 ctgactgcat taccaaaacg ttttgaagtt gtggaacaag gccgatcgat tttaagcggc
    10801 tgtatcttgg aattggatga tacgacaggt catgcaaaag aaatccagct gattcagatc
    10861 aacgatgatc gaccgttcat ggagtagaaa gagaggcagt tttttgcagc aggagccgga
    10921 aattcaaaaa gaagtaagga aactgacaaa acttttaaga gagaatgaaa cgatcattcg
    10981 ctacaaagaa cttgaagaaa agatccagca aaaccagtat ttagctgagt tgagagaaaa
    11041 aatcaaacaa gcgcaaaaag atgctgttca ttttgcccac tatgacaaac cagcagcaga
    11101 aaaagaagca atcaaacaag cggatcaatt catgcaagaa tttgatcagc atccgcttgt
    11161 tgtagcttat agaaaacagc tgttggaagc agatgatctg cttcatcatt taacaactat
    11221 gatacaagaa gaaatcaatg gacaaataga ggaggaaaaa catgcctcaa aaaactaaaa
    11281 atactcctat gatggaacaa tatctaagca tcaaggccca ataccaagat gcttttttgt
    11341 tctatcgttt aggagacttt tatgaactat tttacgaaga tgcgatcaaa gcgtcacagc
    11401 ttttggaatt aacgttgacg agcagaaatc gaaatgcaga agaacctatt cctatgtgcg
    11461 gtgtacctca ccatgcagca caaggatata tcgatgcctt gattgaaaaa ggctataaag
    11521 tagcgatttg cgagcaggta gaagatccaa agacgactaa agggatggtc aagcgcgaag
    11581 tgatccagct agtcactcct ggtacagtca tggacagcaa aggattgtcg gcaaaagaca
    11641 acaatttttt aacagctgta gttagtcaag gaaatgaatt tggctttgct tatgccgatt
    11701 taagtacggg agaattgaag acggctcggt tgcatgatga agaagctgtt ttaaatgaag
    11761 cctctgcatt gcagacaaaa gaactcgtat taggcagtga gatcactgat acccttcgtg
    11821 aacagctaag cgggcgttta gggctagtct tttctcaaca aaatgagatg gaagaaaacg
    11881 cggaatttag ctttttgaca agcgaactag tcgatccgct ggaaaaagaa gcaaccggca
    11941 aactattgac ttatttatcg gtgacacaaa agcggagttt agctcatatc caaaaagcag
    12001 tagaatacca accagatcat tttttgaaaa tggattatta ttcaaaattc aacctagaat
    12061 taagccaatc tattcggact gggaaaaagc acggcactct tctatggttg ttagatgaaa
    12121 caaaaacagc aatgggtggc cgtttactaa aacaatggct agaccgtccg cttatccagg
    12181 caaaacaaat taaagcaaga caagaaatgg tcgcttcttt gctagacagc tattttgaac
    12241 gtatcgattt gcagtcagca ttaacgaaag tctatgatct ggaacgccta gcaggtcgag
    12301 tagctttcgg taatgtcaat gggcgagact tgatccagct gaaaacttct ttagaacaag
    12361 tgccaatgat cagagggttg attgaaggaa tcgatcaagg tcaatggcaa agtctgcttt
    12421 cagaaatgca gccgatggac catctcgtcc aattgatcga ccaagctatc caagatgatc
    12481 cgccgttaca aataacggaa ggaaacatca tcaaagatgg attcaatgag cagctcgata
    12541 tgtaccgttc tgctatgaga aacggaaaga aatggttggc agaacttgaa gcaaaagagc
    12601 gtcaagaaac aggaatcaag aacttaaaaa tcggatttaa ccgcgttttc ggttattata
    12661 tagaaattac aaaagcaaat cttggaaatg cagaactgga gaagtatgaa cgaaaacaaa
    12721 ctttagcaaa tgcagagcgt ttcatcacac cagaactaaa agaattggag acacaaatcc
    12781 tcgaagcaga agaaaaatct gttgatttag aatatcagtt gtttttagct gtgagagaag
    12841 aagtcaaaaa ggctatccag ccgctacaag tacttgcaaa agcaatcagt gcagtagatg
    12901 tgctacaaag ttttgcaacg atcagcgaac gctatcagta tgttcggcca gaacttgtca
    12961 gtgacaagca tcagcttctg atcaaagacg gacgacatcc tgtagtagaa aaagtattag
    13021 gacatcagga atatatccct aacagcgtag aaatggcaga agacgaaatg atcttgctga
    13081 ttacaggacc gaatatgtct ggtaaaagta cgtatatgcg acagttggca ttaactgttc
    13141 tgatggcgca aatgggctgt ttcgttccgg caaaacaagc agttttacct atttttgatc
    13201 ggatttttac ccgaattggt gcaagtgacg acttaattgc tggacaaagt acttttatgg
    13261 tagagatgat ggaagccaat caagccctgc gttatgctac gccaaacagt ttgattttgt
    13321 ttgacgaact agggcgagga acagcaacct atgacggaat ggcgttggcc caagcaatca
    13381 tcgaatatat ccatcgtcat gtacaagcaa aaacactctt ttctactcat tatcatgaac
    13441 tgacggtact tgagaaagaa cttccgcagc ttaaaaatgt ccatgttgga gccgttgaaa
    13501 aagacggaga agttgtcttc ttgcataaaa tgatggatgg acctgcggat aaaagctacg
    13561 gcatacatgt ggcaaagatc gcaggattgc cttccaatct actagaacga gcagcagata
    13621 tcctgcatac gctagaaaat ggcgataacg gacaccatac tgttccagct gaagtagaag
    13681 agaaaactaa aacagccaaa caggagcaaa taaaaccacc tgcagaaaaa gtaaatcaag
    13741 accatataca agaccgtgta aaagaagaaa accaacagct ttctttattc agtgaattat
    13801 ccgaaaatga aacagaagtc attgaatcac tgaagcaaat aaatttacta gaaatgacgc
    13861 caatggatgc actaaacaca ttatatgaat tacaaaaaca actgtaggaa gaaggcggat
    13921 atcgatggcc aaaatccaag aattatctga acgtttagct aatcagatcg ctgcgggaga
    13981 agtagtggag cgaccagctt ccgttgtgaa agaattagtg gaaaatgcaa ttgacgccgg
    14041 cagtactcag attgatatct tagttgaaga agcgggactg cggacgatcc aagtgattga
    14101 taacggagaa ggcattttgg cagacgacgt agagaatgcc ttcaagcggc atgcgacaag
    14161 taagatccat aatcgcgacg atctttttcg catacgaacg ctgggctttc gaggggaagc
    14221 tttgccaagt attgcctctg tttctgaaat gactctggaa acggcgaatc aggaagaaaa
    14281 acaaggcacc tatctgtcgc tgaaaggcgg agaagttatt gagcatcgac cagcaccact
    14341 gcgtcaagga acgaagatta ccgtcagcaa tctgtttttc aatacgccag cccgcttgaa
    14401 atacgtgaaa acactgcaca cagaattagc gaacatcggg gacatcgtca atcgactggc
    14461 attgagtcat ccatcgattg cttttcgttt agtacatgat ggaaacaaaa tgctggcaac
    14521 tgctggaaat ggcgatctga aacaaacaat tgcgggaatc tacggattat ctaccgcaaa
    14581 aaaaatgctg aaaatcgaag gaaaagatct agattttgag ttatttggct atgtttcatt
    14641 gcctgaggtg actcgtgcca gtaggaatta cttatcgacg atcatcaatg gacgttttat
    14701 caagaacttt tccttgaata aagcaatcgt tgcaggttat gggtcaaaat taatggtcgg
    14761 tcgtttccct attgctgtat tggaaatcaa aatggacccg cttttagtag atgtaaatgt
    14821 tcatccgacg aagcaggaag tccggctgtc gaaggaaaaa gaactaacag agctaattag
    14881 taacgccatt caaaaagcat tgtctcaaga aaatctgatt ccaagtgcag cggataatct
    14941 acggtttaag aaaaaagtac ctgatcagcc taaaacagaa caaatcgaaa tcgatttgtc
    15001 ctacgctttt gaagaagaaa aacctaaaaa gccaagtagt ttgaattttg atcgagagac
    15061 aggtaccttt ttcgtagaag aactaaagaa cacatttgtt gacagaattg ttgataagtc
    15121 tgtggaaaat gttgaaaact attctggttt ttctgaagaa aacaccactt atccagaaaa
    15181 aacagcgaaa acggtggata agtttggtga aaatgttgat aactttgtag aaaaaacagt
    15241 ggaaaaatct gaaaagaaag aatttcccat tgtggataac atggagaaaa caaatgaaga
    15301 acaagcagat aaatccgtag aagaggatga aaagatttac gaggaagaat tgacactcca
    15361 tcctgaattt gatttcactc aaggaaacga caaaacatta gaacaagcca tcaagaaatt
    15421 agaaacggaa aaaccagtac aacgttttcc tcagttggaa tattttggac agatgcacgg
    15481 aacgtatctt tttgcccaaa gtagcgatgg gttgtacatc attgatcagc atgctgccca
    15541 agagcgcatt aagtatgaat atttccggga aaaaattggg gaagtatctg atgatctgca
    15601 agaattgctt gttccatttg tgttagacta tccaaacagt gatgctttga aattaaaaga
    15661 gcaaaaacac ttgctagaag atgcagggat ttatttagaa gaatttggtc aaaacagctt
    15721 tattgtacgg gcccatccta cttggtatcc caatggagaa gaagaatcga ttattcggga
    15781 gatgatcgat atgctgctga caactggaag tgtgagtgta aaaaaattcc gtgaagcaac
    15841 agcaatcatg atgagttgca aacgatcgat taaagccaac cattatttga atgaagcaca
    15901 agcacgtgtg ctgctgaagg atctggcacg atgcgaaaat cctttcaatt gtccccatgg
    15961 ccgtccagta ttgattcact ttaccaattc ggatatggaa aggatgttca aacgaatcca
    16021 agacccgcat taagtcaaag agaagaggga aaacatgaag attgtattag catcccaatc
    16081 accaagaaga aaagagttat tggctcatat cgtttcagaa ttcgagattg aacctgctga
    16141 tatcgatgaa acgccatttc cagacgaaca gcccgtcgat tatgttcgta gaatggctga
    16201 agaaaaagca cgaactgtct gggaacaaga tgaaaaagaa gaccatttag tgattgccag
    16261 tgacacgact gttgttctca atcaagagat catgggtaaa ccagaagatc tggctgaggc
    16321 tgaattcatg ctgaaaaaac taagcgggga aacacatact gtatatacag ctgttgtttt
    16381 aaaaacaaag gaacgagaag aacggatact agcagaagca catgtcactt tttatccttt
    16441 gacagacgaa gagatcaaac gctacttgga gacgggggat tatgcggata aggcaggggc
    16501 atacggcatc caaaatgctg cgagtgtatt cgtcaaagaa atttctggag attattacag
    16561 tatcgtagga tttccgattg gtgctgtcaa tcaggcactg aaaacattca tttaaacaaa
    16621 aaagcggact atctgatttt cgattgaaaa tccatgatag cccgtttttt ttattgaaaa
    16681 gattacttat ctactctgac taagatttta cttcagcttt accgactgct tcgcccgggc
    16741 gaacaagtcc tgattggtca agagaaagtc ctgcaacttt ttcattattg gtcaaagcaa
    16801 caataatgtc agttttcttg ccagcttgtt ctatttctgg caaatccatt tcagcaatca
    16861 gggtctctgg tgtgacaagg tatccttctt tcacgaatac attgaaagga acacccttta
    16921 gttcaacagt atctaatccc atgtgaacaa gtacttccag cccctcatta gaaagaatac
    16981 caatggcatg ttttgtttca aagacactcg tcactttacc gtttactggt gcatagactt
    17041 tgccgtttga cggttcgaca gcataaccgt cacccatcat tttttgtgag aaaactgggt
    17101 catttacttc agttatttcc ttgatggtcc ctgcagcgac tgcgaataat gtttcttttg
    17161 ttgcaactgc cgtaccagtt gctgaaacag gttcgaaaga ctgtggcgtt gctgttgcgc
    17221 catttgctgt ttgaagttgt acatcagcag cattttcagg aacagcagga tcaatcttat
    17281 tcaaaattcc ctgacgtcta aagatgatcg tcaagccaaa tggaatgatg acagcaatga
    17341 tcattgcgat gatgaaaggt acccatgttt ctgcttggat agctagtatt cctggcaaac
    17401 cgcctactcc gatcgcgtta gctcgaacac ccatcaagtt agcaaacatg ccagctaatc
    17461 cagagccgac cattgcagcg acgaaaggat agacgtattt caaattgatt ccgaacatag
    17521 caggttcagt tacgcctaag taacaagaaa tcattgctgg aatcgagatt tgttcttctt
    17581 ttttattccc acgatgtaag aagatgatgg ctaagactgc agagccttgg gcgatatttg
    17641 ataaagcaat catcggccat aaattcgttg atccgaaatc tgcaatcagc tgcatatcga
    17701 tggcattggt catatggtgg agaccagtaa tcactaaagg agcatacagg aatccgaata
    17761 cggcactaaa caaccagctg atagaagacg tcaatccagt atttacgata tttgagatcc
    17821 agcttccgac tgtccagccg ataggaccta aaataacgtg tgcagctaaa acagttggta
    17881 acaatgaaaa caatggaaca aaaatcatcg agatcgactg agggatatat ttgcggaaga
    17941 agatttctag atacgctaac ataaaccctg ccagcatggc tggaataact tgcgcttgat
    18001 aaccaatcat ctggacttga gcaaaaccaa aatcccaaaa tggaatatct gctgcggctg
    18061 tggaagctac actataagca tttaataatt gcggagaaac taacgtaatt cctaaaacga
    18121 ttcctaatat ttgtgtagta cccatctttt ttgcaatact ccaagtgata cctactggaa
    18181 gaaaatggaa aattgcttca ccgggtaacc acaaaaaggc attgacacca ttccaaaact
    18241 tcgagtgttc aacgattgtt ccaccaagac tttcaaactg gataccttca agtacatttc
    18301 tgaatccaag gatcaatcca ccgacaatga ttgcaggaat cagtggtgta aagatttctg
    18361 ctaaaacggc aatagcacgt tgaacgggat gaaggttttg ttttgcagct gcttttcctt
    18421 gttctttgga aaccccttca actccagata ctgcaacgaa gtcgttataa aaagtagaaa
    18481 catcgtttcc tatgatgact tggaactgtc ctgcattcgt gaacatccct tttacgctag
    18541 gtatatcttc gatcgctttt tcgtccgctt ttcctggatc attcaggaca aaacgcatac
    18601 gagtcgcaca atgactaact gcagcaatat tttcttttcc gccgatatca tgtaacagct
    18661 tctcggcatc tgcttgatat tttgccatat caataaaccc ctttctctta acttgtctat
    18721 gcaagttgtt tcttcattta ctatactttt tttcggagag aattgcaagc gttttattaa
    18781 aaagtacgag agaaaatgat atcgttttaa aaagcttaaa ataagctttt cttaaaaata
    18841 ggaacattga aatacaaaaa gagctgtagt atagtgaaga aatgatggaa gaacgagtga
    18901 gggaaaaaac atgaataagt tcaatgaaat ttttttagat ttggaacaga aaattttagc
    18961 gaaagaatat cctccgcatt cgcttttacc cagtgagaac caattgatca aaatttatgg
    19021 tgtttcacga gaaacaatta gaaaagcgct gaatcttttg actacggaag gttatattca
    19081 aaaaaaacaa ggaaaaggtt cgatcgtact ggatcggaat cgcttcgact ttcctatttc
    19141 tggattgact agctataaag agctacagga gacccaacgt attccaagtg aaacgatcat
    19201 ccatacgtta gaagaaacag aagtgacaaa agcattgaac gagatcactg gctgggagat
    19261 cggtgcgcct gtttggcatt tgatacgcga aagaaaaatc aatggcgaag tagttatatt
    19321 agacaccgat tatctattaa aggaaatcgt tcctatttta actccagcac aagcacaagg
    19381 atcgatctat gaatattttg aaaatgagct ttctttgacg attgcttacg cgcaaaagga
    19441 gatcacagta gaagaagtaa cggaaatcat gaaaacagcg atggatcttc acgatgacac
    19501 ttatgcagta gtagtcagaa gcctcgttca tctggaagat acccgctgtt tcgagtattc
    19561 tgagtcggtt caccgattag ataaattccg tttcgttgag tttgcccgac gccgaaaagt
    19621 gtaaatagtt caaaagaaag caaattgttg gcatctgtcc actttggcgc tatgatagga
    19681 tcacaaaggg ggcattttta atggataaac caataagacc agaaaaagaa gaactgaaag
    19741 aaaagttatc acccatggct tatgcagtca cacaagaaaa tgcaacagaa cgaccattta
    19801 gcgggaaata tgatgatttt tacgaaaagg ggatctatgt agatatcgtc agcggtgaac
    19861 cgttattcag ctctgcagaa aaatatgatg caggttgtgg ctggccatca ttcagcaaac
    19921 caataacgaa aagacaagtg cgcgaaaaag ccgatttttc tcatggcatg catcgaatag
    19981 aagttcgaag caaagaagca gattctcatc tcggacatgt gtttaccgat ggaccagtag
    20041 atcaaggtgg tctacgttac tgcatcaatt ctgcctcact gaaattcatt ccatatgaaa
    20101 agatggatga attaggttat ggggaattca aatcacttgt agaataatgg aaatacacaa
    20161 agagcggtac aatacgggga tttactcgat cgtccgctct ttttttattc aagcgattgc
    20221 tatttgaaag aaacgctccc tagactcctt ttttcacaag gtgaaatgtg ctacaataaa
    20281 gggaacgtat gtgtgcaaaa gaaagaggac tagaatatgt atgaatatct gacaggtgtg
    20341 gttaccttta tcaaccccta ttacatagta gtagaaacca atggtatcgg ctaccagatc
    20401 gcactaggaa atccttaccg ctacagtagc aaactaaatc aaaaaataaa aatttatgtc
    20461 catcaggtaa tacgtgaaga tgcgcatctt ctgtatggat ttgattcgct agaagaaaag
    20521 caactgtttt tgcgattagt aagtgtttca ggaatcggtc cgaaaagtgc ccttgcaatc
    20581 atggcaagcg aagaccactc cggtttgatt caagcagtag aatccagtga tgtcacttat
    20641 ttgacgaaat ttccaggagt agggaaaaag acagcacaac aaatgatctt ggatctaaaa
    20701 ggaaaattcg gtgagctgag tatcgataca ccttttagtc tatttgatga agcaaacaca
    20761 aaagacgcta cagctctatc tgaagcaatg gaagctctct cggctcttgg ttacagtgat
    20821 cgagaaatca aacgtgtaga aaaacaatta aaagaagtag ataatcagac aacggatgag
    20881 tatctacgcc aagcactcaa gctaatgatg aaaaaataag gaggagacca aatgacagat
    20941 gaatacttgt tatcaccaga atctggcgaa aatgaactga gtttggaaaa atctcttcga
    21001 ccacagctgc tttctcaata tatcggacag agaaaagtca aacaagaact tcagatttac
    21061 atcgaagctg ccagaaatcg ggaagaacct ttagatcatg ttctgttgta cgggcctcct
    21121 ggattaggaa aaacgacgat ggccatggtc attgctaatg agatgaccgt aaatattcgt
    21181 acgacgagtg gccctgctat cgaacgtccg ggtgacttag tagcaatctt gaatgaactg
    21241 gaagcagggg atgtcctgtt tatcgatgag atccatcgat tacctagagt ggtagaagag
    21301 atgctttatt cagctatgga agatttctat gtagatatca tggtaggaca agggccaacc
    21361 gcacatccgg ttcatttccc tcttcctccc tttactttga ttggcgcgac gacacgggca
    21421 ggtatgcttt ctgcaccttt acgagatcga tttggcatca tttctcatat ggaatattat
    21481 gacgaacagg atctgcagga gatcgtttta cgttcttcgg atatttttca aacagagatc
    21541 attgaagaag gggcattgga aattgctcgc cgatcacgag gaacgcctag aatcgctaat
    21601 cgcttgctca aacgggttcg tgattttgcg caagtccaag gagacgggaa aatcgatcgt
    21661 cctatagcgg atcaggcact tactttgcta caggtcgatc aggctggatt ggattatgtt
    21721 gaccagaaat tgttgcgaac aatgatcgag ttatatggcg gcggaccagt agggttaagt
    21781 acgctttcag taaacatagg agaagagcgt gagacggtag aagatatgta tgaaccgtat
    21841 ctgatccaaa aaggattttt aaagcgtaca cctagagggc gaattgctac tgcttatgcc
    21901 tatgaacatt ttggttacga ctatcatata tgatttttgc ttttagattg gaggaaaatg
    21961 aatgagtgat acaacacgta cgaaacaatt gatcaaaaac gtattgattg atttgtgcca
    22021 aacgcaatct tttcgaaaaa tcagcgtcca aaaaatcgca aacgaagcaa aaatcaatcg
    22081 acaaacattt tattatcatt ttacagataa atacgatttg cttcgttggg tctattgtga
    22141 ggatgcattg cattatttag atccacaatg tctttcactt gataattggg aagagcaagc
    22201 attgaaaatg ctgaaagcaa ttgctgagaa gaaagaattt tatgcaaata ctgtgctttc
    22261 tgacccagaa attttacaaa aagagttttc tcaactgatc caaaaatcat ttatcaagat
    22321 ttttgaacaa atggatgaag aaagacaact ttctgcaaga gacaaactat tttatgctcg
    22381 ttttttttct tacggttgca gtggtgtatt agtcaattgg atcacgaatg aatatgaaga
    22441 aacaccgatg gaaatcgcta tgcagctttt ccgtttagcc aaggatacgg aattatattc
    22501 ttatcgtctt tatgcaaaag aagagttgga agaacagtaa tagaaaaatt ttttgttgga
    22561 aaagcaataa tcgctaaaag gagatgggga aatgacaaaa attttatttg tttgtttggg
    22621 aaatatttgc cgttcaccga tggcagaagg cttgctgaga aaacgaatag cattagaagg
    22681 aagggaagca gaattttttg tggattctgc tgctacaagc acgtatgaag ttggaaagac
    22741 accgcaccct ggaacgaaaa aaatattaaa ccaagaaaaa gtagatatga cgaatatggt
    22801 tgctcggcaa atcacacccc acgattttga aacttttgat tggatcattg gcatggatca
    22861 agaaaatgta gaagaactga aacgaagagc accacgctca gcgcagggga aaatccattt
    22921 gtttttaagt agcattcctg gaaaagaaaa agaaaatgtt cccgacccat actataccaa
    22981 taactttgat gagacatatc acttgatcac agaaggcctg aatcattggt ggaatatttg
    23041 gattgatgaa aataaaataa caaacaattt gtaaagttaa cgctttcttt ttaaggcgat
    23101 gttagagcat ttccttgttt ttttgtaaaa tgttaggtaa aataaaatta attaagtaac
    23161 actttcttag ctaaaggagg agtgcattcg atgaacgaag agacagtatc gccaatgaag
    23221 aagcttacga agttagttac tcatacttcg tatcaacttt ttgattcttt tgtaaatgac
    23281 actgaagaac atgctaagaa tgtacgattg caaggaaagt ttattcttga gctgcaaaaa
    23341 gctgctactg aacatagtct agttatctta caagtaagag caaatcgttt gagtagcaag
    23401 tatgaaacga tctcaggctg ggtggcgaca aaaacggtcg aaaaagaccg agtgatgatc
    23461 cgcctacaaa acgaagagca gcagatccgg atggttcctg tagaacaaat tgaaaaaata
    23521 acaatccttt caccagcagg ggaacaggaa cggttatcaa aataatttat accagataga
    23581 aaagcacgta tgagcttgta ccatcaatca ttactaatca aaaataaccg aatgctttaa
    23641 gctgatatca gtaaaacgga acgttttagg atgagtatca cagcatgaca gcattgggtt
    23701 gattagttga atcgattgga tggtacaagt ttttttgata aaaaaaggtc ctgctagccg
    23761 gggagctagc aggataagga gttaaaaatg aaaaagtgtt gttagggttg tttgttggta
    23821 tgcttatata atagtcacta gttgtgaata ttttgtgacg aaagttttcg aaagttgtta
    23881 agtttttggg agcgtcaata attttgtgta aataaattgt cctcctgcaa aataattagt
    23941 tactcagtaa acattgaaac taatgtatcg gttacctgtt gaaaaccttt atggcttctg
    24001 tttagaaatt tttgattgta tgtatcaaaa atgctgacta gaaagcgttc tagtgattct
    24061 tcattttgaa actgctcttt tctacggctg tatcttttaa tttgcttatt gaaagactcg
    24121 attagattgg ttgagtaaat ggttctacga atgctaggtg gaaaatcata aaaagttaat
    24181 aagtcttggt tttctatgag tgactgcgtc actttaggat agtttttctt ccatttctca
    24241 atcatgccgg ataagaaggt attcgcttct tcttttgagt tagcttgata aacagcctta
    24301 aagtcatcac agatttcttt t
//
LOCUS       DBKOIJ010000027        23987 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000027 DBKOIJ010000000
VERSION     DBKOIJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.027"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            12..644
                     /locus_tag="ACV5NJ_000842"
     CDS             12..644
                     /locus_tag="ACV5NJ_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6484956.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(778..903)
                     /locus_tag="ACV5NJ_000843"
     CDS             complement(778..903)
                     /locus_tag="ACV5NJ_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HHY6484957.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1037..1930)
                     /locus_tag="ACV5NJ_000844"
     CDS             complement(1037..1930)
                     /locus_tag="ACV5NJ_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HHY6484958.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2112..3038)
                     /locus_tag="ACV5NJ_000845"
     CDS             complement(2112..3038)
                     /locus_tag="ACV5NJ_000845"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HHY6484959.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3123..3884)
                     /gene="pflA"
                     /locus_tag="ACV5NJ_000846"
     CDS             complement(3123..3884)
                     /gene="pflA"
                     /locus_tag="ACV5NJ_000846"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HHY6484960.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4128..6359)
                     /gene="pflB"
                     /locus_tag="ACV5NJ_000847"
     CDS             complement(4128..6359)
                     /gene="pflB"
                     /locus_tag="ACV5NJ_000847"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HHY6484961.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6636..9086)
                     /gene="parC"
                     /locus_tag="ACV5NJ_000848"
     CDS             complement(6636..9086)
                     /gene="parC"
                     /locus_tag="ACV5NJ_000848"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HHY6484962.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9098..11143)
                     /gene="parE"
                     /locus_tag="ACV5NJ_000849"
     CDS             complement(9098..11143)
                     /gene="parE"
                     /locus_tag="ACV5NJ_000849"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HHY6484963.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11309..11743)
                     /locus_tag="ACV5NJ_000850"
     CDS             complement(11309..11743)
                     /locus_tag="ACV5NJ_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HHY6484964.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11870..12508
                     /gene="plsY"
                     /locus_tag="ACV5NJ_000851"
     CDS             11870..12508
                     /gene="plsY"
                     /locus_tag="ACV5NJ_000851"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HHY6484965.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12640..13515)
                     /locus_tag="ACV5NJ_000852"
     CDS             complement(12640..13515)
                     /locus_tag="ACV5NJ_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HHY6484966.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13597..14388)
                     /gene="codY"
                     /locus_tag="ACV5NJ_000853"
     CDS             complement(13597..14388)
                     /gene="codY"
                     /locus_tag="ACV5NJ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HHY6484967.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14406..15806)
                     /gene="hslU"
                     /locus_tag="ACV5NJ_000854"
     CDS             complement(14406..15806)
                     /gene="hslU"
                     /locus_tag="ACV5NJ_000854"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HHY6484968.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15820..16368)
                     /gene="hslV"
                     /locus_tag="ACV5NJ_000855"
     CDS             complement(15820..16368)
                     /gene="hslV"
                     /locus_tag="ACV5NJ_000855"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HHY6484969.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16405..17310)
                     /gene="xerC"
                     /locus_tag="ACV5NJ_000856"
     CDS             complement(16405..17310)
                     /gene="xerC"
                     /locus_tag="ACV5NJ_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HHY6484970.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17508..18884)
                     /gene="trmFO"
                     /locus_tag="ACV5NJ_000857"
     CDS             complement(17508..18884)
                     /gene="trmFO"
                     /locus_tag="ACV5NJ_000857"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HHY6484971.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18853..20931)
                     /gene="topA"
                     /locus_tag="ACV5NJ_000858"
     CDS             complement(18853..20931)
                     /gene="topA"
                     /locus_tag="ACV5NJ_000858"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HHY6484972.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21053..21910)
                     /gene="dprA"
                     /locus_tag="ACV5NJ_000859"
     CDS             complement(21053..21910)
                     /gene="dprA"
                     /locus_tag="ACV5NJ_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HHY6484973.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21966..22733)
                     /locus_tag="ACV5NJ_000860"
     CDS             complement(21966..22733)
                     /locus_tag="ACV5NJ_000860"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HHY6484974.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22726..23586)
                     /gene="ylqF"
                     /locus_tag="ACV5NJ_000861"
     CDS             complement(22726..23586)
                     /gene="ylqF"
                     /locus_tag="ACV5NJ_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HHY6484975.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23846..>23987
                     /locus_tag="ACV5NJ_000862"
     CDS             23846..>23987
                     /locus_tag="ACV5NJ_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHY6484976.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFW"
BASE COUNT         6258 a         5151 c         4130 g         8448 t
ORIGIN      
        1 ttggagatat tatggaaatt caacgtagaa ccactaattt tttacattta ggctcttttt
       61 ttgcaacttt gacgatctta ttttttctat gttattcata ccttaagacc tcacatgcgc
      121 cttctagtgc attaggctgg ttgcttttgg atcattctga tatcacagaa gccgaagaaa
      181 atccgttttt tttgattttg tcttctcttt gcggcttact gttttctgtt tatttcggtg
      241 cctatttttt tcatcgacat cctggtacgt taaacgaagc ttcaagcgta ttgattgcat
      301 ttaaaacgtt ttctttttta gcaattcttt tttattcgtt taatcacaat acgcttttct
      361 tctttatcat gaatggttta gtcgtgattt tatccatttt tacttatttt attttttcac
      421 tcatttttag ccaattgaaa tgtccgctct tctttcgatt agttcctgtc acctgtttta
      481 tttacagctt gttttccttt tttgtccttg ctttaatgcc actgaccaac ccttcgacga
      541 tccaagtcgc aggaaatctg ttggatatgc tcttcatcgt cagtctaggt gtatttatgt
      601 ggcaaagaaa aaacaaacaa aataaaaagc attttgaagc ctgactatta cgacttcata
      661 aatgcttttt tagttttaaa cacttcaatc taaaagaata acccaagatt ggataaatta
      721 ttaatgactc gccattcgtt tcttagtgat tctggattat caaatggact tcctaatcta
      781 atcagctagt tcttcccata aaatgccata cttgactgaa tcataatatt gattatttaa
      841 aaatctaact tttcttatca ctacttcctt tgtcattcca catttctcac catttttttg
      901 cattcctttg tttccagacc atgtagtgaa tctaataggt ggtagatagg caaaatactc
      961 aaataattct tttaaccata aaggaagaga aaggctcctc ctgcaagagt cagtataatt
     1021 tttgcttact gctctcttag gttttactca ctaaaactgt tccatattct ttgatataag
     1081 aaaatgcatg cggtataaat ttcttgctca gctcagaaaa gatttgtcgt ttaagaaagg
     1141 ctatttcctc cattgtcaat gaaggatcca atgttggtaa ttcaatctca aaattgtttt
     1201 tttcgacaat aggtaattct aatgtgaatg attcttcttc ctttatagta ttcgtcacaa
     1261 gtagagactg atggaaaata ctaggccggc gatttttata tttattctta cgtatcctgt
     1321 caacgataac ttctatttga tcaaaaaagt acgattcatt tacgtctcca ataagttcgg
     1381 ctaattcttt gaatgccttc gttacaggaa gctgattctt ctgtacaaaa aagtaattag
     1441 ccaaagtaag cgggaagtgt tctatagagg aaagtgtgat aaaacggtac ttctcatttc
     1501 ctgcagttgt cctcagcaat tctgtctctc ggctgtttct tactccataa aaatacgctt
     1561 ctttccgttc atcttcgaaa caatacgccc aaataacttc tcccatcgct aatttttgtt
     1621 catcaacaga gagtctcttc aattgttcag ctatggtttc agcagccgaa gtggcttgtt
     1681 gcaagtaatc tttaggatca aatattggaa agttcaaatg atatcttcgt tcttccctct
     1741 tgattaatcc tgcatcgatc aataaatcaa gaaaatggga aaatttattt tgcgaaaatt
     1801 cttttttcaa ttctcttaga atcgtttctt gttctcgatg ttcatataga taatcggtca
     1861 atgaagaaaa aagcggatca tcggtcagtt tttgataaaa gttttcctta ttttttgaat
     1921 aatagttcat atatgttctc ttttctatcc gttttatttt acttaaaagg cttttacgta
     1981 aaaaggagga agaacaagag tcataattga ctgttattct cctccatttc ttcggccttt
     2041 agtatgttct atgtttcttt ttatatcaat cgcttatcca ttcattttga atgcttcata
     2101 ctaccatttg attagctaaa tacagccgtc aattgaggta cgacttgttt tttacgtgaa
     2161 acgacacctt taagcaaagc acggttgttg gtcaaagttg tttggaaagc ttcttctact
     2221 ttttcttttc ctttaccagc aacaagtaat tctgaatcac tgtctaagat gtttgtgaca
     2281 actaatacga acagatcgta gccatttgct ttcatttcat cttccattgc tgcttctagc
     2341 tctgcttgtc gatccatcac ttcattaagg tccactgtat tgacttgagc gatacgaacg
     2401 ttggaactgc ccattgggaa actttttgca tccaaatcaa gaagaaccgc agctgattta
     2461 tcacttagat ttgttcctgc tttcaacatt tcaagtccat aagtgtttag atcgacttca
     2521 gcgattgctg caagttcttt tgcagctgct tcatcttcag gcgtacatgt tggtgatttg
     2581 aacaacaatg tatctgaaat gatagctgag agcatgattc ctgcgatttc tttaggaatt
     2641 gttacttggt tttctttata tagttttaaa ataatcgtac ttgtacatcc aactggttct
     2701 gcacgatagt acaatggatt agctgtttca aagtttgcaa tacgatggtg gtctactaca
     2761 gctaaaatat ttacttcgct gatatctgaa atactttgtt ggaattcatt atgatccaca
     2821 agcatgacat ctgcattttc tgctttagct gactctacta ctcgtggtgc agatagctga
     2881 aagtggtcta atgcgtattg tgtttcttca ttaggtgtac ctagagcaac tgcttctgtt
     2941 tctttaccta attcattttg caagtgtgaa aacgcaatgg ctgccccgat tgcatctgta
     3001 tctggatttt ggtgaccaaa aactaaaatt tttgacatgt cattcgctcc tttgtgattt
     3061 ctcctattta ataatagcaa aaaaacggaa agaagcaata cttatcttct ttccgatgcg
     3121 acctatcgcg ttaaatattt tgtatactct tctgtatgaa gcaatcgttt agcattttcg
     3181 acacgttctt ttgtaggtgg ttcgatccct tctaatggat atttcaaacc aagtgtttcc
     3241 cacttgtatt ttcccatcgt atgataagga agaatctcta ctctgtctac atttgacaag
     3301 gtttgaataa agttgtctag tcggatcaaa tattcatcat aatcacttcg ctcaggaact
     3361 agtacatggc gaatccaaac cggtttgttg atttttgaca aatattgagc catttctaaa
     3421 atattttcat tgtcccaatg cgttagtttt ttatgctctt cattatcgat gtgtttgatg
     3481 tcaaataaca gtaaatctgt atacttcatc aattcttgaa aacgtgaaaa gaaaggttct
     3541 tcatacgtaa aaggttttcc gcatgtatcc aatgtagtat gcatcttccg ctcttttgct
     3601 tttttaaata gttcgatcaa aaaatcgatc tgcaatagag gttctccacc actaacagta
     3661 ataccacctt tatctcccca gtaaggtctg aagcgttctg ctttatctaa aagttcatct
     3721 gctgtgtatt ctttccctcc gccaatattc caagtatctg gattgtgaca aaattggcaa
     3781 cgcatgcggc atccttgcat aaacacgaca aagcgcagtc caggtccgtc tactgaaccg
     3841 aaagtttcga tagaatgaac atagccgatt gttttttctt ccatttaagt tcacatcttt
     3901 tctttgattt cattttaagt acaagttagc tgtcgaagcg aagctttctg aacaaaattt
     3961 ttcgcaattg ccgcttcgtt atgtgtaaaa tttacaggga cggaacatac aagtcctgcc
     4021 cctgtaaatc gtttctttca aataagcata ctgattattc gattttgatg cgatggctat
     4081 tcaacgtcct ttcgacgcta agtcgaaaaa cttttttcac gaacttttta cattctgtcg
     4141 tgtgaagttc tcatcaagac atctaattgt tgttctcttg tcaagtcacg gaatttaact
     4201 gcataaccag atacccggat cgtcaagttc ggatatttca ttggattttc cattgcatca
     4261 cgcaataaat cattactgaa tacattgaca ttcaaatgat aagctccttt tgagaagtat
     4321 ccatctaaga cattacgcaa gttttcgatt cttgtatctt gttcttttcc aagacctgaa
     4381 ggattgattg tttgggtatt tgaaattcca tccaatgcac agccgtaatt cattttagct
     4441 gttgagttca ggctagctaa taacccactt tgttccgcat tgtaacttgg atttgctcct
     4501 ggcgcaagtg gttttccagc ttcacggcca tcaggagtat ttcctgtatt tttaccgtaa
     4561 acaacatttg aagtaatcgt caataatgaa gttgttggtt ttgagttgcg gtaagtttta
     4621 tgacgtttga tttgtcccat gaagtattcc aagatccatg aaactaattc atctgcacgg
     4681 tcatcatcat ttccgtattt agggaagtcg ccatccactt caaaatcaac tgctattcct
     4741 ttttcattac ggattgcttt tacttttgca tatttgatcg ctgacaagct gtctgccgca
     4801 tgagagatcc cagcaatacc tgtagcaaat gtgcgttgca agtcagaatc catcaaagcc
     4861 agctgagctg cttcataaga atatttatca tgcatataat gaatgatgtt caacgtattg
     4921 acatatagtt ctgctaacca atccatcata ttcatgaagt tatgtttcac ttcttcaaag
     4981 tctagtattt ctgatgtgat tggacgatat tctggtccaa cttgggcgcc agtcacttca
     5041 tcgactccgc cattgatggc atataatagt gttttagcca agttagcacg agcgccaaag
     5101 aattgcatgt cttttccgac aactgttcct gatacacagc aagcaatcgc acaatcatca
     5161 gaaccccatt gttcacgaag ctgattatca ttttcgaatt gaatcgaaga actttcgata
     5221 gcgattctgc tagcatatgt tctgaatcct tctggaaggt gttcagaata caaaacagtc
     5281 aagtttggtt ctggtgaagg tcccatattt gttaatgtat gaagcagacg gaaatcattc
     5341 tttgtaacta atgaacgacc atctaatccc atacctgcaa tggataaagt agcccagata
     5401 gggaatcctg agaataattg gttatattca ggtgtacgcg cgaatttgac catacgtaat
     5461 ttcattacta agtggtcgat caattcttgt gcttcttttt ctgtgattac tccattatcc
     5521 aaatcacgtt ggatatagat atcaaggaat gctgagatac gtccgattga catcgcagca
     5581 ccattttgtg atttgattgc tgctaaatat ccgaagtata gccattggac agcttctttt
     5641 gcatttgcag ctggtttaga aatatcatag ccatatttcg cagccatttc tttcatttgc
     5701 gctaatgcac gatattgttc agtgatttct tcacgtaaac gaatcacttc ttcactcatt
     5761 gtatgataac cagtcatatc atgatctttt ttcttttgtt ccatcaaata gtcgattcca
     5821 tacaatgcca cacgacgata gtcaccgatg atacgtccgc gaccgtaagc gtctggaagc
     5881 cctgtaatga ttttgttttt acgagcagca cgcatttcag cagtataagc gtcaaataca
     5941 ccttgattat gtgttttgcg ccagtcagtg aaaatatgtg tcaattcatc gtttggtgta
     6001 tagccattgc tttttagcgc attgttagcc atattgattc cgccgaacgg catgaatgct
     6061 tgtttcaaag gtttgtctgt ctgtagaccg acgatttttt ccaaatcttt atccaaatag
     6121 cctgcatcgt gtgaagtaat agttgatacg atttctgtgt ccatatcata gacaccgtta
     6181 cgctcgaatt ggatatcatt taacttttgc aattcatccc ataatttttc tgttgcttct
     6241 gttgggcctt ccaaaaagct gtcatcaccg cgatattcag tgtagttatt ttgaataaag
     6301 tctctagtgt ctacgccttc tttccatttg gtacctttaa atccattcca ttgctccatt
     6361 gtatggacct cctaaatttc tgttttatgt aacagttttt acgtgattag tataacacag
     6421 caccctatct ttgcaagggt tttcttgttt tttatttaac aatttttatg ttttatttac
     6481 atatatttaa atcaacgttg acataccaac atttaagcat tgtgaacact ttcatctttt
     6541 tttgcaaacg tataaggatt tattaaaaat gaatgaaaaa gagacgccac ctgtattaat
     6601 acagatggcg tctcttcatt aaaatactac aacagttacg actcgatgaa aatctcagga
     6661 gcatcgataa cttcgatgac ttctccacct tttttctcat ccatcgcaaa tgatccattc
     6721 gatgttcgat cacctatagg gaaatcagtt gggtcaatcg ttaactgctg ccctttttga
     6781 ttcatcaagg ttatttcttt tgatcttcct tccgacatat agataatacg gtgcggattt
     6841 tttttcagct cacgcatgac cgtcactccc cgcttcgcac gtgaagtttg gctgatttct
     6901 tgagcaagca tgcgtttaac agctcctcgc tgagtaacga ttacgatcgg cgtatcacct
     6961 tctgctaaca caagcagccc gttgataatc ctatctccat ctttcaaatt catagacttc
     7021 actccagctg cctttgctcc aacaacggga acatctgtta aaggatagcg caaacccatt
     7081 ccttgatgcg ttactaagaa aacatctaag cctggctgat cttctttcag atacacagca
     7141 atcagttcat cttcttccgc ctttagcttc atacagctga gcggacggct cttataggtg
     7201 cgccatggtt cgaactctgt cattcgtgtt tgcttgatca atccattctt gctgatgaat
     7261 acaaatgttt tctctgcttg aattttttga tatgggaaaa cagctaaaat cgattcgtct
     7321 acagacagat tggagattgt ttgagaaatg tgctcaccag catctttcca tttcaaatcg
     7381 ggtagttcat ggacagggcg gtagatcaca tttgctttat ttgtgatcag aagaacatgg
     7441 tccaatgtac tcaattcacc tgagtatagc aagtagtcgc cttctcgcat cccgatttct
     7501 tctggtttgg aagcactata agaacgaagg cttgttcgct tcacatagcc ttctcgcgtc
     7561 acactgacaa ttacgtcttc ttgtgccact aagacttgcg tatcaatttt gatttcttcg
     7621 atttcgtctt cgattgtcgt tagacgagcg gaagaataat tttttttcac ttctcgcaat
     7681 tcttttttca taacagagaa aagctctttg tcatttgaaa ggattttatt caactctgtg
     7741 atttgcgcaa tcaagctttc ggattccttt tgaagctctg tgatatccgt gttcgtcaaa
     7801 cggtataatt gcaatgtgac aattgcttct gcttgttctt ctgtaaattg aaagacgtcg
     7861 actaaattct ttttggcgtc ttttttgtct ttactttcac gaatcgttgc gattacttca
     7921 tccagaatcg ataaagcttt catcaatcct tctacgatat gctgtctttt ttgagctttg
     7981 gctaaatcga attgactacg ttttgttatg acttgctttc gatgctcgat ataacttctt
     8041 aaaatgtctt tcagtccaac ttgatgcgga gtcatatggt cgatagcgac catattgaaa
     8101 ttgtaattga tctgtagctc agtgtttttg aacaaataat tcaaaattcc ttgagcattg
     8161 gcgtcttttt tcaattcgac gacaatttgc agacctgttc tatcactttc atctcgtact
     8221 tcagctattc catcgatttt cttattcaaa cggatctcat ccattttttt caccaaggtc
     8281 gctttattta cttcatatgg gatttctgtg atcacgattt gctgtttatt acccttcatt
     8341 gcctctatct ttgttttcga acgaagaata acttttcctc gtcctgtttc ataagctttt
     8401 ttgatttcat cttttccttg caagatccct cctgttggaa agtcaggtcc aggaatatat
     8461 tccatcaatt tttcaaggct cgcttgtgga tgatcgatca tatagattgt gccatcgatc
     8521 acttcagcta agttatgagt cgggatttct gttgcatatc cagcagatat acctgtcgat
     8581 ccattgacga gaaggtttgg gtatcgagct ggaaggactg ttggttcttt ttctgtatca
     8641 tcaaaattcc acacgagatc gactgtttct ttctcgatat ctgctagcat ctctccactt
     8701 aacttggata atctggcttc agtataacgc atcgcagcag gtggatcacc gtccatactc
     8761 ccgttattcc cgtgcatttc gatcagtact tcccgcaact tccagtcttg gctcatgcgg
     8821 accatcgctt cataaatact gatgtcacca tgaggatgat aattccccat gatatttccg
     8881 acagattttg ctgatttacg gaatcctttg tcatacgtat tgccatcttt gttcatggca
     8941 aataatatcc ggcgttgcac tggcttcaat ccatcacgga tatcaggcag tgcacgttct
     9001 tggataatat atttggaata acgtccgaag cggtcaccca tcacttcttc taaagttaat
     9061 tcttgtactt cttgtcgatt ttccatcagg tgacctccta ttctacttca aataactgat
     9121 tgttttcatt tttatctgct cgttcttcat ctaataaatc attagaaact gaaggagaga
     9181 tttcagtatc ctctttttta tctaagatac tgccatcttc ttcgagcgta aattgcacgt
     9241 gattttcgat ccattttctt cgaggttcta ctttgtcacc catcagtgtg gttactcgac
     9301 gttcagcttg cgcagcatca tcgatacgga cgcggatcaa cgtacgggaa gtcggatcca
     9361 tcgttgtttc ccacaattgt tccgcattca tctctccaag tcctttatat cgctgcagca
     9421 tatatccttt gccgactttt ttaatcattg ctgccaattc gtcatctgtc cacgcatatt
     9481 caatgacttg cttctttcct tgtcctttgg atactttata taaaggcggt aacgcgatat
     9541 atactttccc agcttcaatc agcggtttca tataacgata aaagaaagta agcagtaata
     9601 cttgaatatg ggcgccgtct gtatccgcat cggtcatgat gattactttg tcgtaattac
     9661 agtcttcgat agaaaactca ggaccgactc ctgcaccaat cgtatagatc atcgtattga
     9721 tttcttcatt tttcaagata tcttgcattt tagctttttc ggtattgatg acttttcctc
     9781 gtaaaggtag aattgcttgg aactttctgt ctcttccttg tttagcagat ccgccagcag
     9841 aatccccttc gactaaatac aattcatttt tcttagggtt tcgggattgt gcgggtgtca
     9901 attttcctga aagaagagat tcgccttttt tacgcttctt cccatttcgg ctttcttctc
     9961 tggctttacg tgctgcttct ctcgcttctc tggctttgat tgctttacgg accaacatct
    10021 ggctcatttc gctattttcc tgcaaataaa agcccatttg ttcactgatg acgttatcta
    10081 ctacggtacg agcaactggc gtaccaagtt tttcttttgt ttgcccttca aattgcagaa
    10141 gattttcagg tacacggata gaaagtacag cagctaaccc ttctctgaaa tcactacctt
    10201 ccagattctt gtcccgttct ttcagcaaac caacttttct cgcatattca ttataagatt
    10261 tcgtcatcgc cgctttcata ccagcttcat gggttccccc atcttttgtc cgaacgttat
    10321 tgacaaaaga taaaacattt tctgaataac cgtcattgta ttgataagcg acctctactt
    10381 cgataccttc tttttctcct gaaaaataaa caactggtgt caatgtatct ttttcttcat
    10441 tcagataatc aacaaattct ttgattcctt cttcgtaatg aaaaatttcc ttgactggtt
    10501 cttctccacg aagatcagaa agagagattt taacgccttt tagaagaaag gcagattctc
    10561 ttaaccgttc ggctaaaatt tcatatgaaa atttcgttgt actgaatatt gtatcatctg
    10621 gtaaaaaatg gacagtcgtc ccgtttgctt tttttgtttt tcctatcttt ttcaaagtac
    10681 cgtcaggttt accgccgttt ttaaatttct gctgatactc tacgccatct ctaacgatcg
    10741 ttacaattag ccatttcgat aaggcattta ccacactggc tcctacaccg tgcagtccac
    10801 cagaagtttt gtaccctcct tgaccaaact ttccgccagc atgcagcaca gtaaaaatca
    10861 cttcaactgt tggtattccg gaagcatgca ttcctacagg catcccacgt ccgctatctg
    10921 caacagttat actattgtct tcatggatgg tcacatcaat ctcactgcca taacctgata
    10981 atgcttcgtc aactgcatta tcgacgattt catagactaa atgatgcaag cctcggctgt
    11041 cagtggatcc aatgtacatc cctggtcttt ttctaactgc ctctaatcct tccaatactt
    11101 ggatggacga gtcattgtat tcgttgttta ctttttttgc caaggcaaac gctccttcaa
    11161 taatactttg cggcaaaacc gcaccacgat tatcatacta gaaaaggtcg tgaaaaaaaa
    11221 gagggtagac gagaaatcaa ttttaattat ctcctaaccg ctttttcgac attcccctct
    11281 atcgatcatc tcttctttgt ggtcattttt atttttggtt ttctttcaac ttaccaagtt
    11341 caatcttaat acaacgatcc attacgatat ctgtacgtcc tgcttttctt agcatctcag
    11401 ctgcttcttt gttttcaatc cctaattgtg cccagaacac tttagcatca gttttcaaaa
    11461 aatcacctgc tatttcaggt aaaaattcac tccgtctaaa tacatctacg atatcgattg
    11521 gatcatcgat ttctgtcaaa ttcgcataga ctttctcgcc tagaatttct tcaccagcca
    11581 ataatggatt tactggtatg attttatatc cattgtcttg caatacctgt gcaaccatgt
    11641 aacttgttcg ttctggttta ttgctcaagc ctacgacggc aatatttttt gcttcttcca
    11701 aatagttgaa aatcgtttct tgtgttggat tttttacaga catttctgcc accccttgct
    11761 tttcttttta ttccatctat tatacttgta tctttcattt tttcaattgt tcctgttacc
    11821 tattcatgct aaaataaagg atagttttca acttgaaaga gggtaaaaaa tgaaattgat
    11881 cttattactt attttagcat atcttttagg ttctattcct tccggtgtct ggattggcaa
    11941 attgtttttc aaaaaagata tccgacaaca cgggagcggt aatactggaa cgaccaatac
    12001 atttcgtgta ttaggcaaaa cagccgggat tgttgtcctg cttatggata ttttaaaagg
    12061 tacattggct acttgtttgc cgatgatctt ccacctttct gggattaacc ctctttggtt
    12121 tggggtatgc gccattcttg gccatacatt tccgattttt gctgggttca aaggaggaaa
    12181 agctgttgcg acaagtgcag gaatgctttt aggatttaat cctgtatttt ttgtttattc
    12241 ttgtatcatc tttatcgtta gtttattttg tacaagtatg gtgagtttga gcagtatgat
    12301 cagtgcagta ttgatcactt tatctacgat cgtccttcct tatgtagctc ctgtgatttt
    12361 ggctaagccc aattggttac tgacgatcat tgccttttta gtttcttcct ttatctttta
    12421 tcggcataaa gacaatatca agcgtatcag aaaccatact gaaagtcgaa tcccgtttgg
    12481 tttgaatagc agcaagaaaa aatcctgata ctttttcatt ccaaagagat aaaacgggag
    12541 tgtgacaaat cgatgtcaca ctcccttatt acttacagga tagctttttt acacaccttg
    12601 gtaaacggtg ttccctgcta acttctcggt cacaacctct tacttcactg tgatggaata
    12661 ttttgtcttg aagatttcat gggcacctag ttgattgatc cctacttttt ccatcaagtt
    12721 tccactactg ttcaccgtgt cagcgattcc gcaccacggt tcgatacaaa cgaaaggcgc
    12781 ttcttttgga taaggagacc agatccctac ataaggcata tctttatagc ttaaagttac
    12841 gctatgggaa gatttttcac ttcgaatggc gtacgcattt agaccccttg tttcaaaaac
    12901 caaggcatct tgctcaaaaa gttcacgatt caaagccaga ttcgtatttg tctgacctaa
    12961 cgtcttttga tccatatcga tgaatgggcc tttcaatggt aggcgtaatc ttgatttcaa
    13021 tggagaaaat gccagataga aatcttcaaa tgacaatcct tcttcaagag gtacattgaa
    13081 agcaggatgt ccaccgatag aaaagaacat ttctttttca ccggtatttg ccacttgata
    13141 atgaaccgtg atgccgtctc ctcctaattc atagctgatc actaattcaa aatcaaatgg
    13201 atacttttct aatgtctccg gcgtactcct taagatgaaa ctagccattt cttgtccatg
    13261 ttcaatgact tcaaaatcca tatcccgagc aaatccgtgt tgccccattt gatatgtttt
    13321 cccttgataa acatactgat catcttttaa acgtccaaca aaagggaata aaaccggtgc
    13381 atgtctgccc caatacgctg gatcaccttg ccagatatac tctaaaccat gttcttttga
    13441 tttcagactg atcaattccg caccgtgttc agcgatttcg gcgatcagct ggtcattttg
    13501 aataataaca gtcattgcca ttcctccaac ttcgacttac tttttaaaat gggaaggctg
    13561 gaacaaacat ttctatctgc cccagtcccc ttacttctaa taactgtgtt ttgctaactc
    13621 gtctttaaaa cgggaattca atacttttaa ataagttccc ttcatgccta acgaacgaga
    13681 ttcaatgatt ccagctgatt ccaattttcg taaagcattg acgatcacag aacgagtgat
    13741 gccgatacta tcggcaatat tagaagcagt cagacgacct tcatctccat ccaatgcttc
    13801 aaagatagct tgtacagcct tcaattcgct ataagataat gtgttgattg ccatttggac
    13861 agccgttgca ctccgtacat ctgcttcgat gcttcgagac ttctgataaa ggatctgcat
    13921 accgacaacc gtagctccat attctgctaa gactagatcg tcatctgcaa acgattgttc
    13981 catacgagat aagataatcg tacccaaacg ttcgccagct ccaaagatcg gaacgacagt
    14041 cgtcagacca aatggatatt tctctcttga ctctaccgga aatgcagtaa tatcactagt
    14101 aattgggata ttagcttcag ttttcatcaa ctgtgccact gtatcagtat aagattcagg
    14161 aaattgtttc tcaacaaaca ttgttttgat tcgttcattg ttcacatcgt gacgttcatt
    14221 ataacctagt aatactccgt cacttcctat gatataggtg ttgctgtcaa gaataccacc
    14281 tagtgtgaca gccattttat cgtaaggcaa atctgcttgc atgtcaaaag tattcttttg
    14341 ctgcaaaagt gtgtttatct tgcgggtttt cgttagcaag tctgtcattg tcgtcctccg
    14401 attttttata aaatatagcg acttaaatct tcatttgccg caattgagcc aagtttgtca
    14461 tccacatatg cttctgtgat cgttatttct cccatttgca tatctggtgc ttcaaataat
    14521 agatcttcta gtagtttttc caaaatggta tgcagtctgc gtgcgccgat attatctgtg
    14581 tcacggttga catcgaaagc gatttgagcg atccgttcaa ttgcttcttt tgtaaacgta
    14641 actgacacat tttctgtacc gatcaacgcg acgtattgtt ttactaaagc attatttggt
    14701 tcggtcagaa tcttcacaaa atcttcagct gtcaaatcat ctaattctac ccgtatcggg
    14761 aagcgtcctt gaagttctgg aattagatca cttggtttag acaaatggaa agctcctgaa
    14821 gcaatgaata ggacgtgatc tgtttggatg atcccgtatt ttgtgtttac ttgtgaacct
    14881 tcgacaattg gcaatatgtc tctttggacg ccttctctgg atacttctcc agattgctgt
    14941 gatttagaag tgatcttatc gatttcatcg ataaaaataa ttccactttc ttccgctaat
    15001 ttgattgcct cactatggat atcagcatcc ttgactaatt ttgctgattc ttccttgatc
    15061 aataactcta aagcttcttt gactgttacc gtccgttcca cttttttctt cggtgtcaaa
    15121 gcacctagag tttcatttag gtcgatcccc atttgctcca atccattgtt catcattggt
    15181 gcaggtttct tttggtcttc aacttcaatg gttacttcac gactatccag taaacccttt
    15241 tgtagttgtt ccaagactgt acggcgattg attcgaatat cttctgtcac ttcttcttgc
    15301 ggttcagcgg tattttgctg actagcatta aaaagattca tcatttgttc aaattgatta
    15361 gtggattgtt tctgttcttt tttgatacca ggtaccaaga tttttaccaa acgtttttct
    15421 gcattttttt tagcacttgt gtatacacgg ctgtactgct ctttttctac gatttggatc
    15481 gcattttcta ctaaatcccg aaccattgat tctacatctc ttccgacata accgacttca
    15541 gtaaattttg ttgcttcgac tttgacaaat ggtgcattga cgatttttgc caaacgtcgt
    15601 gcgatttctg ttttaccgac ccctgtaggt ccgatcatca gcatattttt tggtgtaata
    15661 tcttgttgca ttttttcatc taactgcaaa cggcgatagc ggttacgcaa agcaacggca
    15721 acagattttt ttgctgcgtg ctgtcctacg atatattcat ctaattcact tacgatttgt
    15781 cttggtgttt gttttaattc atacatatct actcacctct tacagttctt ctacgataat
    15841 attatgatta gtaaagatac agatatctgc ggcaatattt aacgcatttt ctgcaatttc
    15901 tttcgcactc atgtcgtttt gagcatagtt tttcattgct cgggctgcag ccaacgcaaa
    15961 attccctcct gatccgatag ctaagatacc atcatctggt gtgataactt ctcctgtacc
    16021 tgaaacaaga agcatttctt tatcattcat tacgatcagc atcgcttcaa gtttttgcat
    16081 agattgctgt gtccgccatt cttgagctaa ttcgacagct gcgcgttgta gattcccgtt
    16141 atattcgttt agtttgcctt caaatttttc ttctaaagta aacgcatctg caacacttcc
    16201 ggcgaaacct acaacaactt ctccattgta gatacgtcgt actttctttg cagtgccttt
    16261 catgacaaca gattcaccca ttgttacttg gccatctcca gccatagcaa atttcccgtc
    16321 tttttcgacg gcacagatag tggttgaatg aaatggtgat tcgaccatgt ttgtttcctc
    16381 ctaaatttgt tcttttcctt atttttacgc tcttggatgg aaagaacgat agtttttttg
    16441 taaactttct tttgttacat gggcatagat ctgggtagtc gataaattgg catgtcctag
    16501 caattcttgg actgtccgca aatctgcccc attatttaat aaatgagtag caaatgtatg
    16561 gcgcagcatg tgaggatgga tatcactatc caaactgctt tttttgatca tttgattcaa
    16621 gacatattca attccagtag gcgtgattgg ttttccatga tgattcacaa aaacgatatc
    16681 atgctcttcg tgatattttg tcatcaatac ctggcggcct tccctgaaat attcttgcag
    16741 agcatcttga gcaaaagaac ctagaggagc atatctctct ttgttccctt taccatggat
    16801 caacatgacc tctgaatcaa aatcaatgtc tgataccgac aaactgctac actcacttaa
    16861 acggatgcct gacccgtaaa ggacttccag taaagcgcgg ttcctcaaat ccaaaggact
    16921 atctcctgct acgctgtcaa aaagtgcttg catttcattt tcataaaaaa aacgtggcag
    16981 tctcaaattt ttctttttga gatgaacgta agaaaatgga ttttctttta tcacttcttc
    17041 tttgagaaga tactgataaa acgaacgtag gctggctatc ttgcgactga tagaatttct
    17101 gctatagcta ggacgatgat cagctaatgc actcaaatag acacgaaccg taaaatattc
    17161 cgccttcagc agatcagcgt ctccagattc ctccaaaaaa tgtttgaaat ggagaatgtc
    17221 ttcttcatat gcttctttcg ttttttctga atatcctctt tcgacaatca aataacgcaa
    17281 aaatttttgt tcccacgtgg cttctgtcat tttctcacct tccttttgtc ccagagaata
    17341 atttagcaca gcgtttataa aaagacaaat aaattgtcag aatttaacca atatttctaa
    17401 gtttgttcac aattggcgca attcctcaca ttttgtttga gaaattctta agaaaaacgc
    17461 aagaaaaaga gcgagactat ccgtctcacc ctcttcatgg agtttcctta ctcatttggg
    17521 attgcagttt tttctaattc tgtttttatt gcctcattag cttttaaagc tcgttgtgca
    17581 attgcttcat atcttgcttt tttgtctcga atgcgctctg gcagttcagg aaacagacca
    17641 tagttggcat tcatcggttg gaaatgtttt ccttccgcat gagtaatgta gtaagccata
    17701 cttccaatag ctgtttcttg tggaaactcg actggttcca tttcttttgc taagcgtgcg
    17761 gcgttgattc cagcaagtag tccgctcgct gcactttcaa catagccttc tacaccagtc
    17821 atctgacctg caaagaacag atcttcgcgt ttcgttgatt ggtaagtcgg tttcagcaat
    17881 tctggcgaat tcataaagct gtttcggtgc atcacaccat agcgaacgaa ttcagcattt
    17941 tctaacccag gaatcatccg gaaaacacgt ttttgctcgc cccatttcaa gtgagtctga
    18001 aagcccacga tgttgtataa agaagctgca gcattgtctt gacgcagctg gataacagca
    18061 taaggacgtt tgccagtttt aggatcttcc aatccaactg gtttcatagg tccgaaaagc
    18121 atcgttttga atcctcgctg tgccattact tcaattggca tacatccttc aaaatatttt
    18181 tctttctcaa aagatttaag ttctgccact tcggcattga ttagtgcctc atggaaagct
    18241 ttgaattcct cttctgtcat cggacagttc aagtaagctg cttctccttt gtcatagcgg
    18301 gacttcaaat atactttatc catatcgatc gtagctttgt ctacgattgg tgcagctgca
    18361 tcatagaaat aaaaaccatc tgaaccattg aatgctttga tgctttctgc taatggatga
    18421 gaagtcaaag gtcctgttgc tactacggtg attccttccg gtatttcagt aatttcttcg
    18481 tttatcactg ttactaatgg atgctctttg acttttcggg taattgtttc agaaaaagaa
    18541 tcgcggtcaa cagctaaggc accaccagct ggcactgctg tttcatctgc cgaagtgatt
    18601 actaccgaat tcaatcggcg catttcttct ttcaataacc ctactgcatt agccaaactg
    18661 tttcctctta atgaattgga acatactagt tcagcaaaat tacttgtgtg atgcgcctca
    18721 gtcgatttga ctggacgcat ttcatataaa cggacaggga ctcctgcttg tgcgatctgc
    18781 caagcagctt cacttccggc aagccctgct ccaacaacat ttaccatttt caatgaaaga
    18841 acctcttttc tattattttt ggatgtcttc ttcgtaatct ccatttacac agactacttg
    18901 tttaccgcct ttgactttct tctcgaccag atatccgtca cattttggac aatttctgcc
    18961 cactggttta tcccaagaag tgaattcaca ttctgggaaa cgggaacaac catagaatag
    19021 tctgtttttc ttagattttc gttcaatgac ttgtccttca tgacatactg ggcaagtcac
    19081 accgatttct tttacgattg gtttcgtatt ccgacaatca ggaaaattac tgcaagcata
    19141 aaacttccca tatttcccta attttatcac catcggatgt ccacagacat cacaatcgaa
    19201 tcctgctggt tcatctttga tttgaatttt ttcgattttt tcttccgcat tcgtcagttc
    19261 tttttcaaac ggtttataga aacgatcaac cactttgacc cacgcttccg tgccttcttc
    19321 aattttatcc aaatctcctt ccatttctgc tgtgaaatgg atatcgacga tttgagggaa
    19381 gaattcacag attaatgaat tgacaatttc tcctagttca gtcggctcaa atcttttttg
    19441 tgtcaatttt acatagtagc gtctttggat cgtatcaagt gttggtgcat aagtggaagg
    19501 tcgccctacc ccgttttctt ctagtgcacg aatcaacgta gcctcactaa aacgtgctgg
    19561 tggttgagta aaatgttgtt ttggttcaat atcaacagat tggactacat cgccttcttc
    19621 aagttctgga agaatatttt ctttctcttc tttgccgtca tctctacctt ccacatacac
    19681 ttgcatgaat cctttgaatt tgatttttga accgttcgca atgaagatga caccattttg
    19741 ttctaatgtc actttcattg tatctaaaat agctggtgtc atctgactag ccacaaaacg
    19801 tgaccagatc aatgtataaa gtttcaattg atctttatct aaatattgtt tgagttcatc
    19861 tggtgtacga agcacactcg ttggacgcac agcttcatgg gcatcttgtg cgccttgagc
    19921 atttttgatc tttcggtgat catgtctaga aaattctttt ccatacgtat cttcaataaa
    19981 tgctgcggcc tctgcttttg ctgtatcggc aatccgggta gaatcagtac gcatgtatgt
    20041 aatcaagccg actgttcctt gtttacccaa agtgattcct tcatataatt gctgtgctac
    20101 catcatggtt tttcttgttc gaaagtttaa cttacgggca gcttcttgtt gcaggctact
    20161 tgtagtgaaa ggattcgcag ggtttctttt gcgctctttc ttttctactt ttttaacttc
    20221 gaagtctgtt ccatcaattc tagcagtgac ttcttttaca ctttgtgcat ctggtaattt
    20281 ctttttctta ccgtcgattc cccagaaatt tgctttgaat ttcttgcgag cttttttaaa
    20341 attaccgtcg atgctccaat attcttctgg aacaaattct cggatctctt tttcacggtc
    20401 aataataatt tttaaagcga cagattggac acgtcctgca cttaaccctt tcttgacctt
    20461 acgccagaga ataggactga tcgaataacc aacgattctg tctaaggtac gacgagcttg
    20521 ctgtgcatct actaaatcta catcgatcgt tcttggttcc ttaaaagctg ctttgactgc
    20581 gtcttttgtg atttcattga agaccacccg atttttttct ttcagatcca acccaagtag
    20641 gtacgctaaa tgccaagcaa tcgcttctcc ttctctatcc gggtcacttg ccaagtaaac
    20701 tttttgagct ttttttgctg cggctttcag gcttttgatg acatcgcctt ttccgcgtat
    20761 agaaatataa tggggttcat aattgttttc aaaatcgata cccattttac ttttcggtaa
    20821 atctcttata tggccaacac tggccattac tttatagttt cgtccaagat atttctcaat
    20881 tgtcttggct tttgcaggtg attctacaat cactaaatat ttatacgcca ttactgaatg
    20941 gactcctttc cattgtcgtg ttcctactat taaaagagga ttcatacaaa tcgtagctaa
    21001 tcatatccat tcagaaaacg tttgtcaaga aaagcaaatg aaaagtatgg tgttattctt
    21061 taaattctgc caagatatct tcacccgaaa ccgtgcaaac tgcaccgtct tggatcaaat
    21121 gatgacatcc ggtagaatgt tctgttaaaa tatttcctgg aacagaaaaa actgttcttc
    21181 caagatccaa tgcttgctgt gctgttatca gagagccgct ttttttcttg gcttcaaaga
    21241 cacatgttcc tttagacaaa gctgcgataa tccgatttct cattggaaaa caatgctttt
    21301 tcgggccgga ttgaggagga tattcactca aaagaagatg atcatttgca atcacctctt
    21361 gcaagtcacg attttcagca ggatagactt gctctatccc tgttccaatg actgcgattg
    21421 tccgtccacc atgagagatt gctgcttggt gaccgtatgt atctatccct ttcgcgcagc
    21481 cactgacgat cacatatcca gcttctatca ctggttttag taaataataa actgtttttt
    21541 tggctaaagc agaagtgtgt cttccaccaa caacgcttaa catgttacaa gaaagtaaag
    21601 atagatcacc attataaaac agtacaattg gcggataggt caagtgaagc agctgagggg
    21661 gatatttggg atcaaagcaa gtgataaacg actgtttcga ttggattttg ccccagttag
    21721 cgtgaatttc ctgccaactt ttgcgaaaca gctcccggtg tttggttatt ttacctatct
    21781 taatgatgtc ttcaaatccg atttctttat aaggatactg catcatccag ttagcaagtg
    21841 cccattttcc ttgactactg attccttttg tataagcaag tttcaataaa ttttcttcta
    21901 tttgatacat cataaaaccg cctttcgtct ctctttctaa taagatacgt aaaagcggct
    21961 gttttttatt gtaaatattt ttttacaggt gcaaaacttt tacgatggat tggcgtaatt
    22021 ccttgtcgtt caagtccttc taagtgttcc tttgttccat aacctgcatt atgcgagaaa
    22081 ccatatccag gataaagcac gtcatattct ttcatcaatt ggtctcgata tactttagca
    22141 atgatacttg cagcagcaat cgacacagat cgtgcatccc ctttgattat tttttcctgc
    22201 tgaataggca aatccaaggt catcgcatcg atcaacaaat agcttggtac aatggacaat
    22261 ttttctactg cttggatcat cgctaattta cttgcttcat aaatattcac atcatcgatt
    22321 gtctgctgat caacgatccc gatcccgatt gccagggctt tttcttgaac ttgttgataa
    22381 agaagttctc gctttttctc tgataattgt ttggagtcat tcagccctaa tatgatctca
    22441 tttttcggca agataactgc agctgccacg acaggtccag ctaatggtcc tcttcctact
    22501 tcatcaatgc cggcaatcca ttggtgacct tgtgcgtacc cttgcttttc aaagcaaaac
    22561 atttcttcat atttttcctt aattaatgct tcctttttca atcgtcgttc aaaagaagcc
    22621 agtgcttgct gcacgccttt gcgtggatca tttcttaatg attctagtgt aggatcatct
    22681 attctctgta tgcttttcaa tcgttctttg atactttgga tggattcagt catcactttt
    22741 ttgctccatt tcttcgcaac gatccagtgt gtatcgtcca agttttcctt gacgaatatc
    22801 atgcacgact aattcgctgc cacgatcata gtcttcttta tacccacgtt ttgctgtaat
    22861 atccatcagt aattccggtg ccaatttttc ttcttgctcg attgataaac cataacgatc
    22921 gctgattctt cctgaataat atcgggcaaa aaagtctaat ccgtaaatcg ctaaatcatc
    22981 taaatggaca agctggtctt tgatcgcgcc tgtcagcgct agttttttgc cgatttcttc
    23041 atcttcaaat ttgggccata aaatccctgg tgtgtctaac aattccaact cattacctaa
    23101 acgaagccac tgctgacctt ttgttacgcc aggcttattt cctgtctgtg caattttctt
    23161 gcctactagt cgattcatca acgtagattt cccaacattt ggaataccta agaccattgc
    23221 ccgaatagcg cgaggcttga tcccccgagc agcatctctt gcccgttttt ctttcaatgc
    23281 ttctttagcc gcaggtacta gacctttaat tcctttactt tcttgtgccg tgatagctaa
    23341 tgcaagatgg ccttgtttac gaaaatattc ttgccacagc tttgtctgtt gtggatctgc
    23401 cagatccgct ttattcaata aaacaaggcg aggtttttgc tgaatgatct gatctagcat
    23461 tggattacga gaagataaag gtaatcgtgc gtctaccaat tcaaatacga tgtccacata
    23521 tttcattttt tcagaaactt ctcgtcgggc ttttgccata tgtcccggaa accattggat
    23581 ggtcattgtt ctctccattc ctttcatacc ggaatattcc ccggctttac ttgttttttt
    23641 ctttttcaat ataaattcac ttttttatta taaagaaatt ccatctatta gttaagtgaa
    23701 agtgaaccag tcctcattct atcacatctt cacttttttg atgatgcaaa tttttatttt
    23761 ggtttctata tatgaatcta aatttttgac agcgtttcct aaataagaga tgatagacga
    23821 acaaaacaga aaggagacga aacaaatgaa tagtatggtt tttgttaatt ttcctgttgc
    23881 tgatgtcaat cgttccgttg ctttttatga aaagctgggt tttaaaaaaa atgaagaatt
    23941 ttctactgaa gaagctggtt caatggtatg ggatgacact ttttgga
//
LOCUS       DBKOIJ010000028        23936 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000028 DBKOIJ010000000
VERSION     DBKOIJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.028"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            57..3335
                     /locus_tag="ACV5NJ_000863"
     CDS             57..3335
                     /locus_tag="ACV5NJ_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HHY6484977.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3876..5783)
                     /locus_tag="ACV5NJ_000864"
     CDS             complement(3876..5783)
                     /locus_tag="ACV5NJ_000864"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HHY6484978.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5913..6788
                     /locus_tag="ACV5NJ_000865"
     CDS             5913..6788
                     /locus_tag="ACV5NJ_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HHY6484979.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6868..7842
                     /locus_tag="ACV5NJ_000866"
     CDS             6868..7842
                     /locus_tag="ACV5NJ_000866"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HHY6484980.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8022..9167
                     /locus_tag="ACV5NJ_000867"
     CDS             8022..9167
                     /locus_tag="ACV5NJ_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HHY6484981.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9304..9648
                     /locus_tag="ACV5NJ_000868"
     CDS             9304..9648
                     /locus_tag="ACV5NJ_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HHY6484982.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9919..11205
                     /gene="efmA"
                     /locus_tag="ACV5NJ_000869"
     CDS             9919..11205
                     /gene="efmA"
                     /locus_tag="ACV5NJ_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HHY6484983.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11343..11942)
                     /locus_tag="ACV5NJ_000870"
     CDS             complement(11343..11942)
                     /locus_tag="ACV5NJ_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HHY6484984.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12346..13467
                     /gene="mnmA"
                     /locus_tag="ACV5NJ_000871"
     CDS             12346..13467
                     /gene="mnmA"
                     /locus_tag="ACV5NJ_000871"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HHY6484985.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13713..15134
                     /gene="arcD"
                     /locus_tag="ACV5NJ_000872"
     CDS             13713..15134
                     /gene="arcD"
                     /locus_tag="ACV5NJ_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HHY6484986.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15148..16266
                     /locus_tag="ACV5NJ_000873"
     CDS             15148..16266
                     /locus_tag="ACV5NJ_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HHY6484987.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16326..17618)
                     /locus_tag="ACV5NJ_000874"
     CDS             complement(16326..17618)
                     /locus_tag="ACV5NJ_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HHY6484988.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17768..18328
                     /locus_tag="ACV5NJ_000875"
     CDS             17768..18328
                     /locus_tag="ACV5NJ_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6484989.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18457..18834
                     /locus_tag="ACV5NJ_000876"
     CDS             18457..18834
                     /locus_tag="ACV5NJ_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HHY6484990.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18767..20839
                     /locus_tag="ACV5NJ_000877"
     CDS             18767..20839
                     /locus_tag="ACV5NJ_000877"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HHY6484991.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21050..21997)
                     /locus_tag="ACV5NJ_000878"
     CDS             complement(21050..21997)
                     /locus_tag="ACV5NJ_000878"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHY6484992.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22243..22707
                     /locus_tag="ACV5NJ_000879"
     CDS             22243..22707
                     /locus_tag="ACV5NJ_000879"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HHY6484993.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22769..23812)
                     /locus_tag="ACV5NJ_000880"
     CDS             complement(22769..23812)
                     /locus_tag="ACV5NJ_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HHY6484994.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7774 a         4393 c         5050 g         6719 t
ORIGIN      
        1 taaattaggt aatactttaa gtaaacgctt tctaatttgt tacgggagga atacacttgt
       61 taaaaaaacg tataaaatac agtgcgctaa tgatgaatct ccttatgctc agtaccccgg
      121 ttttaatttc tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg
      181 cacacgtgaa cattgaaggg atgtcaacaa tggaggaaat accaagaact agtgaagata
      241 tggatacgga agaaggaaat gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa
      301 agcaatctga agaagcaaat gaagagtgga aaggggttgt tttcggagaa agcacaagtg
      361 ctagtaggta tgccattgaa ccattagaag acggcgtgcg gctctcttcc acgaataacg
      421 gcggaaaatt ccagtcaagc ggatcagatg gaatgaccta ttattatcaa gcaatcccca
      481 aagacatcaa ctttaggatg cgggcgacga tcgaaataga atcttggacc tatacgaatg
      541 cccaagaagg atttgcttta atggtaagag acgcagtacc aaaaaatcat ttgtttgggc
      601 aggcatttta ttcaaattca tttgccatac taggcagtcg gatcgaatat gtctgggact
      661 cggatagaca agaagtggtc aacacgagcg gcagcaaata tacgatgcga cttggattgg
      721 ggacacgggc aatcacagga atatcatctg aagatccgca agctgcgcca gctcctggga
      781 gtgtctcagt ggaaacaaca cctttagaga cttcagcagg gttcttggaa aaagaaacag
      841 gttcttataa tatattcgga aacggtcatg ggaatctgtt tccggccacg aactcgatta
      901 ctaaactgga cgtagagatg aaaaaaacga atactggttt agaggcatat tattacgatc
      961 ctgaaacagg agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt
     1021 cagatgaatc ggttatttat gcaggatttg ctgcagcaag gaatatgacg atcaaggtag
     1081 aaggaatcga agttgcttac tcagatcctg cgacagatcc tcctgggcaa gaaagaccta
     1141 ctcgcttgat tgatccgatt tacactgtga cctctagtaa tcattcagga aaccaagatc
     1201 atactatgtc ctttagagca aatgctgacg gattattgac aattaaaaat aaagcaacag
     1261 gagaggtgct taagaacgca aaaaacttgg ctatcactgc gaatcgggaa tttgattatc
     1321 agacaaaact gatggaagga acgaacgaat ttactttcag ttttacgcca gataaaaact
     1381 atccgccaga agaatacgta gagatgacta gttatgaaac aaaagagatt cttcatacag
     1441 tggactaccg taaacctaaa tattcgacag tttatgtaac accagaaggg tcagatgcag
     1501 gaaacggaag cagacaaaat ccattatctc tgacacaagc attgagatac gcatatgctg
     1561 ggcagacaat cgtcatggcg cctggaactt attccttcga aagaggattg acgattccaa
     1621 aaggcgttaa cgggacaaga gaaaacccga ttcagttgat tgcagagaaa aatccagaaa
     1681 acaagcgtcc tgtacttgat tttaaaggaa ctggaaatgg gatgacactg ttcagtcact
     1741 actggggttt acgagggttt gacattacaa atagtgcagc tatgcaaaaa ggattacaga
     1801 tttctggaaa tcataatttg atcgaagaga ttcatgctta tcggaatgga aatacaggaa
     1861 tccaaatcag cggtagctca aatgatcctt tcgaagcatg gcccgcgcat aacttagtca
     1921 agaactgtac atcttttgaa aatgccgatc caggatttga agatgccgat ggatttgcgg
     1981 ccaaactgac aataggagaa ggaaatgtat ttgacggttg tatcgcgtat cataatgcag
     2041 atgacggttg ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca
     2101 aaaactcagt tgcttatcga aacggctggg taccaggaat tgagggagaa ggtaacggta
     2161 atggattcaa aatgggcggt tcttctttaa cgggccctca tgaattgatc aatagtttgt
     2221 ctttcgaaaa tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt atcgtcaata
     2281 gcagtacaag ttttaataac ggttcatata atgtggctct ttatacctct tcagcaggaa
     2341 acacagacta ccatgcttct ggtatcttat ctttccgaac agagaatctt gagatgagag
     2401 agcagatccg cccattgaat caaaacgaag atcaaatcta ccactcgttg aactactatt
     2461 ggaatgaacg agagaaacaa tcagaaaata ctcttggaca taccatatca aaagattgga
     2521 tcgagtctct aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg
     2581 gaagtatcca tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg
     2641 aaagagtgga tggtcttcca ttatctgtag gtgctatttt tgacggagaa acctcaccgt
     2701 cttcggaata tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac
     2761 aaaataatcc gattattata caagaagcaa atggtgtggc ggagaatgca gaaggaaata
     2821 tcattgaact gaagcctttt acagtttttg tgtcaacttt cacaccaaat caaggaacgc
     2881 ctgtctttga agatatgcgt tatttcccag aaaagatcca aatcgttgaa acacagtcaa
     2941 gtgctcaatt ttctaatcat accgctattt tttcagcacc ttcaaaagga gaatatacgc
     3001 tagaagtcac ttatcgttcg gaaatatttg atggggaaaa atggtctgct catacagaat
     3061 ctgttaccca gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg
     3121 acaaaccaga aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac
     3181 cttctcatcc ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa
     3241 aaacgggaga atttaataac ccgttgctag cattagctgg aggaattttg ttaatcggtg
     3301 tagttgttta tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt ttatgatgta
     3361 aaacaattga ctgaggaaag taaaaataga aggaaatgag accaatagat gtctcacttc
     3421 cttctatctt tttatagatg aggttctcct tgctgctttt gattggctgt tcttattact
     3481 tgatgcagga cagctttttc acaatctctc cactaaacgg tgttcaatca gctgaccctc
     3541 ggtattagct caacaaacgg caaaatatga ggaactattt ttccgttttt ttctctaata
     3601 ctcaggtcag gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa gccagtttct
     3661 cggtagtaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac
     3721 tacttgaaac taagcagctt tttcacaacc tctaatacat ggtattccag aagtaacttc
     3781 ttcgaaaaat aagccgaaat gtcacaaaaa tttgagaagc gattttccca aattccttct
     3841 tatttcttga agctgaccac ttctggcaca acctcttacc agattcgcac tcttttttct
     3901 ggcgctagat acatagcgtc ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg
     3961 ttttttggtt gcacgtttgc tcggagttta gcaggggcat ggacatcgat ttgcagtaaa
     4021 agttgttggt attctttttt tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat
     4081 tcctcgatag aaagatctga ttccgttttt gctgcttcca atgcacagct tagaccgcct
     4141 gcatcagcaa tattttccga aacggttaat tttccgttta cttttccgtc agcaaaaggc
     4201 agaccatcga attcttcgat cattgcttgt gctttgtttt gaaattgtgt aagatcttct
     4261 tctgtccacc agttgttcaa gttgccatat tcgtcaaata aagcgccgtt attatcaaat
     4321 gcatgggaaa tctcatgagc gataacagct ccgattccac cataattagc agagcttgat
     4381 tgttctagac tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat cacattccga
     4441 aatggatgat aataagcatt gaccgtgtca gcactcattt cccattctga acgatccact
     4501 ggtttgttcc atttgctgaa acgatctttt aatgtgagtt tgctgaaaac aagagtattt
     4561 gaaagcaaag taccaccctg atctgcaggt actgttttga actggtcgaa taatgctggg
     4621 atcttatcgg ggtagcctac ttggatacct aagttattta attttatgac ggctttttct
     4681 tttgttgcat ccccaagcca gtcattttct tctagacgtt ttttataaac ggcgatcatt
     4741 tttttcacca tttgttctac gtctttttta gcagcttcgc caaaatattt ttttccgtag
     4801 taatcgccaa ctacttgatc gaattgtcca gaggcaaggt aatacgctgc ttttttctga
     4861 ggcattgctt cgtctgttcc agacaatacg cgagaataga ttccgctgac ttggcggaat
     4921 tcttctgata gataaccact aagagaacgt actgttttaa tgatcatcca gcttttgaat
     4981 aacggaaaat gagcttctgt caggatgtca ttcaaatgat cgaagtacac tggatcagtt
     5041 acgatcactt tatctggagt tgtcccaatc aaacccgtga caagtttttt caagtccagt
     5101 ttatctgtgt atttaataaa tgtatcaaaa gattgcggat tatacatttt gctgtaatct
     5161 gcgttttctt ctgcactttt tacatgagga gcaatcaatt tatcaaattg cactgcttgt
     5221 tccgtgattg cttctgcctg acttttttct ttgccggcaa gctctaataa ctgaacagcc
     5281 atatcaaaga aaacgcctat tagttgtgca ccgttgggat ggtctgatgc ataatacgtc
     5341 ttatctggaa ggaataaaga aggcggataa gcaaagaagg cgttcgtttg cgcattcttc
     5401 atatctgcat ctacatccag attaaatggc aatggtaatg tatccaacag ccattctgga
     5461 aattgcttat tcaattcgtc gaaagactgc aatgattcaa ctttttcgat tataggttga
     5521 agcggttgag catgcaactc atttcttcgg tcataatcgt ttgccagttg ataatatgca
     5581 aggaaatgtt tcattttttc tgaagacact ttttctggat gggcaagcag atcgtctgtt
     5641 tctcccatca aaagttggtc gatcccgtca actaaatctt ggaaaccgcc tgtagctggt
     5701 ttatctgcag ggatttttgc tgttttttgc cattcttcat tgactgcttc atagaaatct
     5761 tgtttcaatc gttctttgtt cattcatcga tcgctccttt tttgtttcgt gtgcccatta
     5821 tcttacaaaa tgaaggaatt aacaaataga acataaaaaa ttgaaaattc gaacaatcaa
     5881 actgatttgt ttttttcaat ccttcactta taatgaaaaa aaggaggcga tcccaactga
     5941 atcaagtagt ggataaacct ttatatttca aagtagataa aaaaattaaa aaattagcca
     6001 gcactcagca gctgcagtca agaaaaagta aattgctttt tttagctctg gtttttgaag
     6061 atcaaagtta tgtgattatc gatcaatcag gacatccggt agaatattct ccagcaaaat
     6121 atacttatca agagggtctt tcttgtaaaa aatggaagct gatcaatgaa gagccaatcg
     6181 agttatctag atggatcaac agaaaagaag atattcccgt tttgatcgaa gaaaaaaggt
     6241 caggaaagga actcgcaaat tgctgggtgg gattgccaga agaacgtttt ttgaggtata
     6301 agaaatgggc taccccctca ggctatcttt gtggtactta cgcggcagca gttctccttg
     6361 cctattatca agactatcgt aaggggtgga tgcttccaaa tgagattcgc aagaaaaata
     6421 cggctgattc gttggttttg acgaaggcat tgcgtagcca gatccaacca ctaggacttc
     6481 ctacgattcc ttttcaggtt agtacaggga tttctagttt tttaaagaag aatggcaatc
     6541 atgagcgtgc acgagcaact cttctaggtt catggcagcg agctacaaaa cgtatacgtg
     6601 aaggcaaacc tgtgatgatt ggtattttga aagtacttgg cagtacatat ggcaatcatt
     6661 gggtgacagc gtacgcttat tttgaaactg aaacaggaga acgttattac aaagtacacg
     6721 acaactgggg cgattatcat aaagtcattc ctgccagttg gagtaacgga acagtttcac
     6781 ttccttaaga atttgctgaa attaacagaa aatcttggaa aaatatgtta aactatcgaa
     6841 gaatagagaa gttttgggga ggactacatg agtaataagt atcaggatga cacgttgaag
     6901 atttttagtt tgaatggcaa ccgtccatta gctgaaaaaa tcgcgaaagt gttcggaaca
     6961 gaattaggta aaagcgtcgt gaagcaattt agtgatggcg agatttccat caatatcgaa
     7021 gaaagtattc gaggggatca tgtgtatatc gttcaatcaa cgaatgcacc tgtcaatgat
     7081 tactatatgg aactactgat catgatcgat gcaatgaagc gagcaagtgc gaaaacgatc
     7141 aatgtcgtat tgccttacta tgggtatgct aggcaagacc gcacggcgaa acctcatgaa
     7201 ccgatcactg cgaaattgat cgctaacttg atcgaagaag caggggcaac tcgagtactg
     7261 actttagatc tgcacacggt tcaagtacaa ggattctttg atattcctgt agataattta
     7321 tttacgatgc cgttgtttgc tcattattac cgtcaattag gtctgacggg agatgacatc
     7381 gtagttgttt cgccaaaaaa cagcggtgtt cagcgtgcac gtagtctttc agaatatttg
     7441 aacagcacat tagccatcgt agatcatgca gatgatgctt caactgaggg tggttatgtg
     7501 atcggtgatg ttcaaggaaa gacatgtatc atggtagatg atatcttgaa cacaggagca
     7561 acgcttgcac gagcagcaaa tgtattaaaa gaaaacggcg caaaagaagt atatgcctgt
     7621 gcgtcacatg gattattgtc cgatcatgcg aaaagtattt tagacaatgc accaatcaaa
     7681 gatatttgta tcactgattc tgttttcaca gaagaaaatc gtcatccaga aaacttaaca
     7741 atcgttacat gttcagaatt aatgggtgaa gcggtcaaac gcatccatga aaatacaccg
     7801 atgagtccgt tattccgtct ggaagaaaaa gaattcgaat aaacaaaaaa aagagggaaa
     7861 gaagttgtga cagaagtgag cagcttcaag gagctgcgac aagacttttg tcacagctcc
     7921 ttttttctat gcccatgaaa cgtcctctag acaaaaatga gctcttcttt ggtttgttcg
     7981 ctattttatg ctagaatgtg cctagcaata atttggaagg gatgaatgaa gtgaaaacta
     8041 tttatctgga tcatgcggca actaccccca tgcatccaca agtaattgaa gcgatgacgg
     8101 caatcatgca agacacgttt ggaaatcctt caagcataca tggatttggt cgccacgcgc
     8161 atgaaaaatt ggaaacagca agacaaatca ttgcagacag tctgcaagca aaacctcacg
     8221 agatcatctt taacagtggg ggaacagaag gggataatac agctatctta gaaacagcgt
     8281 tctcgcgtca aaaagaaggc agacatctaa tcactacagc tatcgaacat cctgctgttc
     8341 ttaataccat gaaatattta gagacaaagg ggtttgacgt tacatatctg cctgtagatg
     8401 aaaaagggaa tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat acgatcttgg
     8461 tctctgtgat gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg
     8521 aacttttagc ggatcatcca gcttatttcc atacagatgc ggtacaagcg tacggaaacc
     8581 aaacgatcca tccagaagaa ttaggaatcg atttattaag tatctctgct cataaaatca
     8641 atgggccaaa aggcgtcggt ttcttatata aacgagacgg agtaaatatc cctgcattgc
     8701 ttttaggcgg ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta gcagggatct
     8761 ggggtatggc aaaagcagtt gaactgctgt cacctgaaga acgtgaagca cgtaacaaca
     8821 aatataaagg attccagcaa ttgattcttg atacattaga agcaaacgaa atcccttatc
     8881 aagtaaatgg tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg
     8941 aagcaaatga tttgctactg atgaagcttg atcttaatgg ggtcgctatt tctactggtt
     9001 cagcttgtac agcaggaaat atcgagcctt cacacgtatt agaagcaatg tatggagaag
     9061 atacaccgat actaaaagaa tccatccgta tcagctttgg tttgggaaat accgaagacg
     9121 acatccagta tttccttgat caattagttc gtgctgtcaa aaaataacta gtgaagtcat
     9181 tcttatatga acagatgtct gagagaacgc cttcattttt tagggaactt aactgaaaaa
     9241 cctagtaaaa atcaggataa acttttataa taaagagaaa ctaattttat cgaggtgaaa
     9301 atcatggcat ttgaaacaac agcaacggta ctgggcgcac caattagcta tcgtttgcat
     9361 cctgatgcaa aaagatatac attgcgcgat aatggattta cagaaacaaa cggaggcaat
     9421 tatcaattga tccgaccatt agatgccacg ccgcaaagca aagaaggatt caaattaaaa
     9481 atcactgtat caaaagatat tcaaacattg aaaatgtcga tcactacagc aaatggattg
     9541 aaagcagtca atattttcaa agatccaaaa cagaaaatga gccaagataa attttatttt
     9601 ctgatggatg ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata
     9661 aaaagacaac tagttttgaa acggcctatt gttagatgac taactttgta aggacgacgc
     9721 aaattagttg tcttttttat ttttaaattt aacacatgtt gttattttta atattttgct
     9781 gatttattgt ttctaagtaa agaaatttaa attgaaacga ttgttattta gggaaaaagg
     9841 tattttttga aaaaagaatc ccgtataatg attgttgtgc aaacgctata caaaatgaaa
     9901 aagaaaagag ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga
     9961 aattatttgt tctttttatc gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa
    10021 tatgcgatca tctggtattt gacaagagaa accggttcgg cgacaatttt gagttttgct
    10081 actttactag gaatgattcc catggtttta ctaagcccat ttgtaggacc gctagtggat
    10141 cgctgggata aaaaagctct tttgattgtc acggatatta ttgtagcgat tttcgcgctt
    10201 attttagcag tagtcggaac gatttcagaa tccttcccga tttggctagt ttttgtgtct
    10261 ttgtttatgc gttcggtcgc acaaacattc caaatgccaa cgatccagtc gattatgccg
    10321 acaatcgttc catcttctca cataacaagg acgaatggac agttaggaat ggtccaatca
    10381 gcgaatttca tcattgcgcc agctctaggt gcggctttgt tttcagtcgt tcctgtcaat
    10441 taccttattt tattagatgt attgggggca gtttttggtg tcggcttatt gatttttgtg
    10501 aagatcccaa aagtttctcc tgaaatattg gaagttccgc ttactatttt taaagatgca
    10561 aaatttgggc tgcagcaatt gatggataat aaagggttat ggtacataac gatcaatggt
    10621 gcatttgtga tgctgttgtt catgccagca atcagtctgt atccattgat gacgctagat
    10681 tactttggtg gttccgttgg acaggcggga gcagtggaag tagtctacgc agtcggaatg
    10741 ctcttaggcg gtgcactgat cagttttata ggaacatgga aagaccgaat gaaacctatt
    10801 attatagcct acatcattat gggcctgacg atcggtgcaa gcggattggt tccaaacgat
    10861 agtcaagggt tcttgtactt tcttatcttg aatgcaggag caggttgtgc aacgccatat
    10921 ttcaatacac tgctgatggc aatgatccag cagagttatg aatcgaatgt tttaggtcgt
    10981 gtcttaggga acttcaactc tttgatgaac ctggcaggtc caattggatt attatttgca
    11041 ggaccattag ctgatcgttt aggggtagaa aaaatgttct tgttttccgg aatcgggatt
    11101 ttattatgcg gaatcgtttt atttttaacg tcagctgccc gaaattatga taaagaatta
    11161 cagaaaaaac tggtaaaaga acaccatgag caaaaagacg aatagtatgg aatggagttg
    11221 tgacagaagt cttgttacaa ctccatttct gatcctaatg ttcctgtggc aataagccta
    11281 ctcaaatgaa aaatatgagt aattatcttt atttgtttaa tacttgatat agggcagttt
    11341 tttcacgacc tatcgtactt cctctttgct tcatagattt ctttgatttg ttgctcagat
    11401 aacggagtgc caaatggtag tttttccttt aagagttctc tttccggtgc gtctacgcct
    11461 acattgacat cgttctcgat aggtacgctg taatgatgga tatgtccatg aagattgcgg
    11521 atagtgtcgt taggcccgag caacatcgga taatgagtca agtaatattg ttgatggtcg
    11581 aatttaacga tggcacctac atcataaaaa taaaatttgg gacttccgtc agtaagccta
    11641 ggatcatttt tttctaaata acggaaaaaa gcgcgactgt catggttccc tttgatcaag
    11701 tggattgttc cttgcagttg agtgaccagt tccaaaattt ctgcatttgc tttttcatat
    11761 tgcggatgca tagcaaggtc gcctagatga taaacatggt cggtttcttt tattactgca
    11821 ttccagctag tgatcaactg ttggttcatt tcatccacat ttgcaaagag ccgcggagca
    11881 aagtcgtttt ttcctaataa gcgttcatgg aaaaagtgca tatcactgat aaagtaattc
    11941 atgtatcttt tgcctccctt ttttcttaga gtaaacaaaa tcacttatct gagcaactca
    12001 taacatctaa aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat
    12061 tttgaaaaat agtttttcac aatttataaa attattcctt attgccgaaa ggctggccct
    12121 ttttatgagt atgccaattt tttatatcga atgctcaaaa gatgagtgat tttttggttg
    12181 actatcatgt ttgtgtactg aataggtcca gtagaacagt atttttacga cttgataatt
    12241 aagggatgaa attttcagaa atatgacgta taatgtaaaa tactgaaaag ttgcgacctt
    12301 caactcgtag atgtttcaga atctaattga aatgatggtg atatcatgac agacaacagc
    12361 aaaacacgcg ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac agcacttttg
    12421 ttaaaggaac aaggatacga cgtgatcgga atattcatga aaaattggga tgacacggac
    12481 gaaaacggtg tatgcacggc cacggaagat tataaggatg tagcgaaagt agctgctcaa
    12541 atcgggattc cttattactc tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa
    12601 tatttcctag ccgaataccg cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa
    12661 gaaatcaaat tcaaagcatt cttggattat gcaatggatc ttggcgccga ttatgtagct
    12721 actggacatt atgcgcaagt cactcgcgat gaaaacggcg tggtccacat gcttcgtgga
    12781 gtagacaata ataaagatca aacttacttc cttagccaat tatcacaaga acagctttca
    12841 aaaacgatgt tccctctagg cggcatggaa aaatcagaag tacgggcaat tgctgaacgc
    12901 gcaggccttg cgactgctaa gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag
    12961 aacttcaaac aatttttgag caactatctg ccagcaaaaa aaggaaatat ggtcactctt
    13021 gatggtgaag tcaaaggaca acatgctggc ttgatgtatt atacgattgg tcaacgtcaa
    13081 ggactaggga tcggtggcgg aggagattcg caagaaccct ggtttgttgt aggaaaagat
    13141 ctagcgacaa acacattata tgtggggcaa gggttccatc atcctgcatt gtatgccaca
    13201 agcttggatg caagtgaaat ccactttacg acgaatgaac caatgccaaa agaattcaag
    13261 tgtacagcga aattccgtta tcgtcagcaa gacgttcctg tgacagttcg cttattggat
    13321 gataatcgtg cagaagtcgt attcgacgaa cctgtacgtg caatcactcc tggacaagca
    13381 gttgtatttt atgatggaat ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa
    13441 gaaacaaaag ttttacaata cgtttaagcg atattaaatg aatcaaaaca gctatggcta
    13501 acaaaagaaa caccttttct tttggatagc atagctgttt ttttgcaata aaattaattt
    13561 ttattcattt tatacttgaa atacttgtgt aaaaagttca taatatccaa taatatgaat
    13621 aaagttgctt tttgttaagc aagagcagtt gcaaaataga agggaatctt ttgcaagtga
    13681 tttgttcaat tttataagtg agggtgatgg aaatggaaaa gaaatcgacg ggtattagca
    13741 aatggggact ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca
    13801 caagtgatct ggctagttct gcagctccag ggccagcgat cctttcatgg ataatcgttg
    13861 ggattggtat tttagctttg gtattatcgc tgaatcattt aggagaaaag cgtccagatc
    13921 tagatggtgg tattttcggt tacgcggaag cttcatttgg taaattaggt ggcttcatca
    13981 gtggttgggg ttactggttg tctgcgtggc tgggaaatgt tgcttttgca acgatgctga
    14041 tgagcgccat gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt
    14101 tatttgcaag tatttttatt tggggtctga ctttattagt aaacaatgga gttgaaggag
    14161 cgagcgtcat caatacaatc gtgacgatct gtaaattagt gccattattc ctatttttaa
    14221 tatttgtatt tatcgcattt aaagtgaata tattcactgc tgatttttgg ggtaacgtat
    14281 ctgataatct agtgaatgga acagggcagc aaggaacgat ctggttgcag attaaaggtt
    14341 gtttgatggt tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc
    14401 gcgcgaagaa acgttcagaa gcccagcaag catcgatcat tggtctgctt tgtttattgt
    14461 ttatctatat ccttgcttcg atcttgccat acggcgtctt atctcaagaa gagttagcaa
    14521 ctatttcaca acctgctatg gcaaatattt taaaaggaat cgtgggaaat tggggagcag
    14581 cgctgatcaa tatcggcttg attatttcga tcattggttc ttggttgtct tggacgatgc
    14641 tcccagcaga aacaacgatg ttgatggctc gagacggtct gttgccgaaa ggttggggaa
    14701 aaacgaataa aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc ttgactaatc
    14761 tattcttatt gactttctta gttactgatt atgcctatca atttgcctat tctttatgta
    14821 cagcggcaat tttgatttgt tatctacttg tcgggatcta tcaagtccag ttttctttaa
    14881 aagaaggaat caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt gaattgatgg
    14941 gcatcatttt agcaggattt tcttatgtcc ttttatgtag tattgcctat cttcctggtt
    15001 tttgcttcta ctggatcgct tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag
    15061 taatgattgg gctgatttgt gtggccgcac tgctttcaat cgggttgctt gctgtaggct
    15121 ggatcaagat ataaggggga aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg
    15181 ctaaataaat gggaaaagaa agcggtatat gatattgcac aaagctcgat cgaagcattg
    15241 acattagacg aactcgttgg cttagatggg actagcgtaa gtaaattttt cgaaaaacgc
    15301 aaaaacgaac cattggacta tggctggatc gaaggatcag atgttttcaa acaagaagtc
    15361 gcttctcttt atcaaaccgt cgatccggaa aacatcttac aaacgaatgg agcgactggt
    15421 gctaatcttt tagctctata cgcgttagtg gaagcaggag accatgttgt ttcgatgctt
    15481 cctacttatc agcaattata tgacatcccc aaatctttag gtgccgctgt tgactttgtt
    15541 catttgaagg aagaagaaga ttggcagttt gatttagaac agctagaagc cttagtcaaa
    15601 cctgaaacaa aaatcatctg tttgaatagt gcaaacaatc ctacaggcac attgctagat
    15661 cggaaaactt tagaaaaaat cgcagaaatt gctcgtacag tagatgcata tgtattgata
    15721 gatgaagtat atgcgccttt aacggataaa ggtgaattct tgtcgatcgt agatgtctat
    15781 gaaaaaggta tagcaaccaa ttccctatcc aaaacctact ccattccagg tattcgtatt
    15841 ggctggacag ctactggacc tgagttggca gaagtttttc gaaagtatcg agactacacg
    15901 atgatttgcg gcggagtcct gtcagatgat cttgctgtac atgcattgaa gaataaagaa
    15961 aaaatattgg cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt aaaacaatgg
    16021 gtagccaatg aaccgaaagt tgaacttgtc gctcccaatt acgtctctac ttctttcatt
    16081 aaattagcca tcgaagaaga tgaccagaca ttttgtatca atttattaga agaaacagga
    16141 gtactattag taccaggaag tgcctttgat ttaccgaagc atgcccgact aggttactgt
    16201 tgtaaaaaag aaaccttgga aaagggatta ttcctcttat ctgagttctt gaaaaaacag
    16261 gcctaaacaa aaacactgca aggacagtct gtgacaactg ttcaagcagt gtttttcatt
    16321 tacgcttatt gtctatgaga aggagagtgc cctgaagcta tggagactcc tgaagctttt
    16381 tcttctgcag tttcttgctg atggatctct tgtgtattgt gtcgtacaag caagaagttg
    16441 accaaaacaa gaagagaagt cgaaagaaag acgccgcgat aaccaaatgc actggaaaca
    16501 ctggagccga tcataggccc tactacgtta ccagttgctt ggaaagattg gttataactg
    16561 aagatccgtc cagcagcttg atgaggcgaa tattttgtga tcaatgcttg gacagcaggg
    16621 agcagacaag catcagagat accaatcaaa aaacgtaagg cagctaattg ccaaacattc
    16681 gtgacgaatg ccattgggat ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt
    16741 ctttcactcc caatcttgtc acctaagcga ccaaatcttg gagcggctat caaagtggca
    16801 atcccaggta tcgaagcaat gattccgctg atcaacgtaa cattcccatg tccatgcagc
    16861 aattggcgaa tgtacagact gatgattgga ctaatggaat tgttggatgc ttggatgatc
    16921 atcgttgtga cgaacatacc gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt
    16981 ttggcagaaa ccatgttttc ttttttcact gggacaaatt tttcatggac gaaaaacaga
    17041 ctcaacaaga agacgaaaaa taagatgatc cctgtaatga aaaaggtagg gcgatagcca
    17101 aacgctgaag cagagatccc gccaagaagc ggtccaagta atgttcctgt aacagaacca
    17161 gttgctaagg ttccaagaac ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg
    17221 gcagtggcat tactgatata accagaaaaa atcccttgaa gcagacgcaa tgcgactaat
    17281 tgatacacac tagtcacgca acccatcaaa gaaatgacga ttgccattcc taatgaagca
    17341 cgcaacagca tcaattttct tcctttttgg tcggctaatt ttccccacca aggagagata
    17401 agcgtagtca ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac
    17461 gtactataat tacctaacgt atctatgtaa agtgacataa aaggcatgac taaactaaat
    17521 ccgataccag ccataaatgt tccaaaccat aagacaaaca agttttgttc ccaagtttct
    17581 cttttcctaa ataatttttc ctttattctt gcagccaatt agactccttc tttctgaaaa
    17641 ttttcatcta cagctcttta ttataaacta caatatgtag tttatcaatg aaagtctctt
    17701 ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta tgagaggaaa
    17761 catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct ttcgagaact
    17821 agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac gcttggatat
    17881 caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa cagcgctaac
    17941 agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg
    18001 tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt caaatcaagc
    18061 agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat tagatggaat
    18121 ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa cgaaagaaga
    18181 aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt ccttacgttt
    18241 tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt tttggatgat
    18301 caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca attttaagat
    18361 gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa aagtgactaa
    18421 gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat taccatgttc
    18481 ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga agaagaagaa
    18541 acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat
    18601 caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg gaatgacgga
    18661 gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg acttgcgttg
    18721 ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga aacaactaat
    18781 tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa
    18841 gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg gtcatttatt
    18901 aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa tggacacgtt
    18961 gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac atcaattaaa
    19021 agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc
    19081 cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt tcaataaacc
    19141 agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg gattcaagca
    19201 actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc gggcaaaagc
    19261 ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg cggataattt
    19321 atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag aaaagaaaat
    19381 cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa aagaacaagc
    19441 caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt
    19501 tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat taaaaacgga
    19561 atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc aacgctatgg
    19621 ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc ataagatcgt
    19681 tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta cgatgaaaga
    19741 acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga aaaacggagc
    19801 catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc atgcactttt
    19861 gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa aaatctatca
    19921 aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca gccaaacaaa
    19981 agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg tcgtccaagc
    20041 aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa aaggaatgag
    20101 aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac cattgtcaga
    20161 agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta cgcgtgcaga
    20221 gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag gactgcaagt
    20281 atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa caccagattt
    20341 gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac aagcaggaac
    20401 cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca aatcaagcaa
    20461 acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg gggccaatct
    20521 gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag attgcaacta
    20581 ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat
    20641 ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg gaacgacaga
    20701 aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag aacgacgctt
    20761 gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag cagcactaaa
    20821 agcagcaatg aagaagtaat aagatatgaa agaggttgtg atagaagtgg tcagcttcaa
    20881 gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt cggcttattt
    20941 ccgaagaagc tacttctgga acaccattta ttcggaaaaa aggactgtga caagactttt
    21001 gtcacagtcc tttttcattt aaaaaaatgt ttacttcatt tgacgtatat tattggtgag
    21061 gcttcaattc atttggcttc actttttctg tttctcttat gatataaaca ggacgctgtt
    21121 tcgtttctaa gaagattttc ccaatatatt tcccgatgat tccaagacat aacaattgga
    21181 ttccaccaat gaacaggaag atcgagacca tggaaggcca tcctgatgta ggatcaccaa
    21241 acaacaatgc ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca caagacaaag
    21301 cgccgataaa tgaagcaatc gttaacggtg catccgagaa gttcacgata ccatcaatcg
    21361 agtagttgaa taatttccag aaggaccaag atgtttcgcc agccacacgt tcgcggtttt
    21421 cataagaaat atattctgtt ttaaatccga cccagctaaa taatcctttt gaaaatcggt
    21481 tgtattctgt taattctagg acggcatcca ccatttgacg agtcatcaag cggaaatcac
    21541 gagcgccatc gaccatttct gtatcgctga ttttattgat caactggtag aattttttcg
    21601 caaagaaact gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg ccgacacaat
    21661 cgatatcaga agtagtgatc cggttgatca tctcaggaag cagttcgggc ggatcttgca
    21721 gatcaacatc catcacggtc actaaatctc ctgtagaagc ttgcaatcca gcgtacagag
    21781 ccgcttcttt cccaaaattt ctggaaaatg agacaaagcg gacataagga taagctgagg
    21841 ctagcttctt gattgctgct aacgtaccgt cttttgatcc gtcgtcaata aacaaatatt
    21901 cgaactcatg ggcacctttg aatttttcaa cctcatcaaa aaaaagagga acagtttttt
    21961 cttcattaaa acatgggaca acaattgata ctttcatcgt ttctatcctt tcaatcgtat
    22021 gtttatatat ggtttacatt caaaatttaa taaaccttaa ataacacctt taaaatttga
    22081 ttttacgtaa aagaacaggt attctttttg taaactaacg tatttagttt accatgtttc
    22141 ctcggtttat caaactttta agaacaattt ttattagttg gcaagtagtt ttggatagag
    22201 aaaaaggagg aagttccagt ctattgacta ggagcaaaca aaatgaaaat acacacacct
    22261 atcggctttt tatggttaga tgcagatgaa tccggattga ctgctgtatc ttttcagcca
    22321 atttcagaaa gcgaatcagc gaatcagcga attttaacgc gcacacaaga acagtttgaa
    22381 gaatattttg cgggaaaaag aaaagaattt gatgttcctt tatctataaa gagaggtacg
    22441 ccttttcagc aaaatgtctg gaaagctttg tcgaccattc cttatggaga aatccgaacg
    22501 tataaagaag tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat tgggcaggca
    22561 aatcgaacca atccattgcc aatcgttatt ccttgtcatc gagtgatcgg gcaaaatgga
    22621 aaactgacag gatatatggg aaacgcaata gatggggttg aaatcaaacg ccaattgttg
    22681 ttgttagaag gatatctgca aaattaaaaa cagttgtgat tcggacattg ttccatgcga
    22741 gaatcacaac tgttttttca tgttcattct aaactttgat cagccaaata aatagtaaag
    22801 ccatcactgt ttggagaagc ccgacagcta atccataaga taagaagcgt gtaccttctt
    22861 tcatgaattt tgcaaagtct aaacgcagac caattgccgc taaagcagtg atctcaaacc
    22921 agctgctgat aaaatgtgcc gattggtcaa taactacagg taaagaaaac aagctgttga
    22981 ccaaacaaaa gatcacaaac cctaatacat accaaggaag cggtaatttt cgttttttct
    23041 gcgtatcagt tggcgtttca aacagagctg gttgtttctt gtgcatgaat ttttcaaaca
    23101 gatagacaac aacaacgagc atcataatac gtagaatctt aaatagcatg gcgaattcga
    23161 caacagaatt gtcaagtaag cttgccccag cgactacttg accaacagat tggagtgtac
    23221 cacccaaaag tgcacttttt tcaatgagat ttccaccaaa caactgtaat ccaagaaaag
    23281 ggagagtcaa catcatcaca gtacccagta gattgactag tgtgatgatc tgtccttttt
    23341 cttcctcttt cgcatggatg gaaggggcga ttgaagcaat agcagaagaa ccacaaacag
    23401 cgtttcctcc agccatcatc aaagaagaag tatcgctgaa ttgcagtttt ttaccaatca
    23461 agtaagcacc gatgatagtg actgacattt gcagtaacac aaaaacaatt ccttttacac
    23521 caatttgagc aatcgtttga gctgtgacag ttgcacctag taaaaaaaca gagaattcta
    23581 atagtttgct ttcagcaaat tttgtgccga ttgctagaac aggctgttta aaaaaagtat
    23641 ttcctaataa aatacccaat aaaatagcaa tagtcgctcc acctaactgt ggcagataaa
    23701 tagcaagaag cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg ggtaagattt
    23761 cgcggaccgc tgaatccttt acagatttta ttggatgtga caatgatttc atttgatcgg
    23821 ttcctttcgt caataaatac taaaagcata ctcgaacata gtagcactaa accagtattt
    23881 tgacaaaaga acaagaggtc gtgacaaaag tcttgtacga cctcttgttt cgaata
//
LOCUS       DBKOIJ010000029        23462 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000029 DBKOIJ010000000
VERSION     DBKOIJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.029"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            70..1731
                     /locus_tag="ACV5NJ_000881"
     CDS             70..1731
                     /locus_tag="ACV5NJ_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HHY6484995.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1801..2148)
                     /locus_tag="ACV5NJ_000882"
     CDS             complement(1801..2148)
                     /locus_tag="ACV5NJ_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HHY6484996.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2232..3173
                     /gene="opp3b"
                     /locus_tag="ACV5NJ_000883"
     CDS             2232..3173
                     /gene="opp3b"
                     /locus_tag="ACV5NJ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HHY6484997.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3174..4217
                     /locus_tag="ACV5NJ_000884"
     CDS             3174..4217
                     /locus_tag="ACV5NJ_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6484998.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4223..5293
                     /locus_tag="ACV5NJ_000885"
     CDS             4223..5293
                     /locus_tag="ACV5NJ_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6484999.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5283..6236
                     /locus_tag="ACV5NJ_000886"
     CDS             5283..6236
                     /locus_tag="ACV5NJ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485000.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6248..7258
                     /locus_tag="ACV5NJ_000887"
     CDS             6248..7258
                     /locus_tag="ACV5NJ_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HHY6485001.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7543..8364)
                     /locus_tag="ACV5NJ_000888"
     CDS             complement(7543..8364)
                     /locus_tag="ACV5NJ_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HHY6485002.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8461..8739)
                     /locus_tag="ACV5NJ_000889"
     CDS             complement(8461..8739)
                     /locus_tag="ACV5NJ_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485003.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9007..11016
                     /gene="metG"
                     /locus_tag="ACV5NJ_000890"
     CDS             9007..11016
                     /gene="metG"
                     /locus_tag="ACV5NJ_000890"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HHY6485004.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11495..12082)
                     /locus_tag="ACV5NJ_000891"
     CDS             complement(11495..12082)
                     /locus_tag="ACV5NJ_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485005.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12257..13579
                     /locus_tag="ACV5NJ_000892"
     CDS             12257..13579
                     /locus_tag="ACV5NJ_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HHY6485006.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13734..14510
                     /locus_tag="ACV5NJ_000893"
     CDS             13734..14510
                     /locus_tag="ACV5NJ_000893"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HHY6485007.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14503..15072
                     /gene="rnmV"
                     /locus_tag="ACV5NJ_000894"
     CDS             14503..15072
                     /gene="rnmV"
                     /locus_tag="ACV5NJ_000894"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HHY6485008.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15065..15949
                     /gene="rsmA"
                     /locus_tag="ACV5NJ_000895"
     CDS             15065..15949
                     /gene="rsmA"
                     /locus_tag="ACV5NJ_000895"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HHY6485009.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16400..17029
                     /locus_tag="ACV5NJ_000896"
     CDS             16400..17029
                     /locus_tag="ACV5NJ_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485010.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17153..18262)
                     /locus_tag="ACV5NJ_000897"
     CDS             complement(17153..18262)
                     /locus_tag="ACV5NJ_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485011.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18471..18893
                     /gene="mgsA"
                     /locus_tag="ACV5NJ_000898"
     CDS             18471..18893
                     /gene="mgsA"
                     /locus_tag="ACV5NJ_000898"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HHY6485012.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19080..19178
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19216..19752
                     /locus_tag="ACV5NJ_000899"
     CDS             19216..19752
                     /locus_tag="ACV5NJ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HHY6485013.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19792..21555)
                     /locus_tag="ACV5NJ_000900"
     CDS             complement(19792..21555)
                     /locus_tag="ACV5NJ_000900"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485014.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21558..23273)
                     /locus_tag="ACV5NJ_000901"
     CDS             complement(21558..23273)
                     /locus_tag="ACV5NJ_000901"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485015.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7561 a         4298 c         4773 g         6830 t
ORIGIN      
        1 tcattaaact cttaaacaac ttcagaaaat attaattatt ctgaaaattc caataaaggg
       61 gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg cggtatgcgg ccttgtactt
      121 gctggatgtt atggcggcag ttcagccaca agcagtagtt cagcaaaaag cggttcagca
      181 gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa tgcctacagc tgacttatca
      241 gttgcaacag atacaatcag ttttacagca ttgaataacg tttatgaagg aatttaccgt
      301 cttgatgaag acagcaaacc acagccggca ggagctagcg agttggcaga agtcagtgaa
      361 gacggattaa cttacaaaat caaattacga gaagacgcaa aatggtcaaa tggtgatcct
      421 gttacagcag cggattacgt ttatggctgg caacgaacag tagacccagc tacagcttct
      481 gaatatgctt acctgtatgc gccagttgaa aatgcagaag acattacagc tggaaagaaa
      541 gataaatcag aactagggat caaagcagtt ggtgactatg aattagaaat caaattgacg
      601 aaacaaacac catatttcca atatctgctt gctttcccat catttttccc acaaaaacaa
      661 tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa gtgataaagc tgtttataat
      721 ggaccattca cattagaaga ttttgatgga ccaggaacag atacagaatg gacttataag
      781 aaaaatgatc attactggga caaagataca gtaaaacttt ctgaaatcaa agtcagtgta
      841 gtaaaagaat cttctactgc attgaactta ttcaaagatg gccaagcaga tgacgtgatc
      901 ttgtctggtg agttagcaca gcaaaacgca aacgatccag cttatacttc tgttaaagaa
      961 gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga attcaccatt caacaatgtg
     1021 aacctaagaa aagctatttc ttattctatc gatcgtgaag ctttagtaaa acaagtacta
     1081 ggtgacggat cagttgtctc tacaggattg atcccatcag atatggtaaa aagtccaaca
     1141 acgaacgaag actttgcaaa agaagctgga aaattagttt cttatgacaa agataaagca
     1201 aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg attctttaga atttgatttg
     1261 atggcttctg atgatgattc atcgaaaaaa gtaatcgaat atgttcaaaa ctcaatccaa
     1321 gaaaacttgg atggcgtaac agtcaaacca acacctgttc cattctctgt acgtcttgac
     1381 cgctcaactt cgggtgattt tgacgcagta ctaggcggat gggctgctga ctatgcagac
     1441 ccaagcagct ttactgattt gtttgtcaca ggtaattcat acaaccgtgg tcgctggagc
     1501 aatgcagaat atgacgaagc agttaaagca gcttcatcaa cagacgctgg cgatgaacaa
     1561 gcccgttggg atgatttcca aaaagcaatc aagatcatct ctgatgatat gggtgtcgtt
     1621 ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa aaatcaaagg tatcgttcac
     1681 catgcggcag gcgcttcatg ggattacaaa tggacatacg ttgaagaata atcgttaata
     1741 ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc aacctcgttc tcatattcac
     1801 ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga gcaattagta ttccggcaat
     1861 gatcagccaa aagctgtaca taccgtagct ggcagacgag agcgaggaaa attctggttt
     1921 tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc tgaaaatgat cgaagaaacc
     1981 cgaagaaaag acccataaaa aaccgccgat aatcagaaac ggcaatgacc ataagaatag
     2041 atcattagat aattgaatga gtgtaaagga atggttgatg agatttttta ttaaaataac
     2101 cagaatcagt agaaaagcta ctgcgtaggg gatagatttt tttttcattt gattgctcct
     2161 ttatttttgg ttcattatct aataatgtag cgaaacatct gtcagaagtc aaaagctagg
     2221 gggaaacatt agtgaatagt tatatcaaat atgtattaaa acgtgtcttt ttcatgatca
     2281 tcacgctttg gttaattgca acaatcacct ttttcttgat gcaattgctg ccgggcacgc
     2341 catatacgaa tcaggaacgt ttgagtcctg aaacaatcga gatgctaaat aaacaagtcg
     2401 gtttggataa accagttatc gtacaatatg ggatttatct ttctaatttg cttcaaggag
     2461 attttggaat ttccttccaa tttaaaaacc agccagtcgc acatttgtta gctggaagaa
     2521 tcggaccatc cttgcagtta gggttacagg caattatctt tggaacagtc ctagggacga
     2581 ttttaggaac gatctctgcc atgaagcaaa atacatgggc agatacatct tctacactcg
     2641 tagcgatatt aggtcgttcc attccaaact ttgtatttgc tgttttatta caatatatct
     2701 ttgcaatcaa attacaagtt ttgccaatcg caaaatggga tggctttgtg tacacaatct
     2761 taccaacaat tgcgttggcc atgtcgccgc ttgctgactc tgctcggttt atccgtacgg
     2821 aaatggtgga agtactgcat agtgattatg tggaattagc aaaagcaaaa ggattgagcc
     2881 gttgggaaat cgcctttaaa catggtcttc gaaatagttt gattccattg atgactttat
     2941 taggaccgtt agctgtagca ttgatgaccg gatcactggt ggtagaaaat atctttgcta
     3001 ttcctggtat cggggaacaa ttcgttaagt caatcacaac aaatgattat cctacgatca
     3061 tggctgtaac gatcctttac tcatttatgc tgatttttgt tattttgatc gtggatctat
     3121 tgtacggtct agttgatcca agaattcgtg tttcagaagg gagccgagga taaatggaaa
     3181 tgataccaaa gcgttatgaa acagttgcag atatccctgc ggatgaattt cttccattac
     3241 aaaaaaatac cgaagaagaa cgtgaacaga ttactgcacc ttctttgaat tttatccaag
     3301 attcatggcg ccgtttaaag aaaaacaaag ctgccgttat ttcaatggtt ttactattgg
     3361 tcatcatttt gatctctgtg atcacaatct ttgtttctcc tcatgatcca actgcccaaa
     3421 acgtggctta tatcaatttg cctcctcgta ttccgggaat caatatcgat ggattgaatg
     3481 gaaaagcaat ggttggtggt gaacttgttg ataaatatgc tcaagcaaac gtcccttcaa
     3541 acgtaaattt ctttttagga acagacggtt tgggacgtga tgtattaagc cgcttattta
     3601 tgggaatgag aatctcgttg ctgatcgcat ttattgctgc attactagat gtaacgattg
     3661 gtgtagctta cgggttggtt tctggtttgt taggcggacg tgtcgataac gtgatgcaac
     3721 gttttttgga aatcttatca ggaattccaa acctcgttgt catgattttg atgttggtcg
     3781 tttttgaacc aggaattttt tcaatcgtgg ctgctatggc catcacaaac tggataccaa
     3841 tggcacgtat cgttcgtgca caaacaatga aattaaaaga tcaagaatat gttttagctg
     3901 gattgacatt aggtgaatca agaatgaaaa ttgcattcaa acatattttg ccgaatatct
     3961 ctagtgtcat tattattcaa atgatgttta gtattccatc tgctattttc ttcgaagcct
     4021 ttttaagctt tatcggttta ggtcttacgc ctccgtcagc ttcattagga acgatgttga
     4081 gcgaagggta caagacattc ctgtatctgc cttatctatt atggattccg gcagtaactt
     4141 tatcggttat catgattggc tttaacttac ttgctgatgg attgcgtgat gcctttgatc
     4201 ctaaaatgaa agagtgaaga cgatgacgaa aattttagaa gtgaaagact tagaaatctc
     4261 ttttgatacg tatgctggaa aggtacgagc gattcgcggt gtcaattttc atttgaataa
     4321 aggagaaaca ttggcgatcg ttggggaatc tggaagtgga aaatcagtga cgaccagaag
     4381 catcatgcgc ttgctgtcaa gcaacgccaa tatagatgct ggtcaaatcc tctttaaagg
     4441 taacgatatc gttggaaaat cagaaagaga gatgcaaaaa atccgtggta aagaaatagc
     4501 gatgattttc caagatccaa tgacctcttt agatccgacg atgcccattg ggaagcaagt
     4561 cgctgagtcc cttagaaagc ataataaagt ttcaaaaaaa gagggacaaa aagctgcatt
     4621 agaattgctt aaattagttg gaatacccga agctgagaaa cgtatcaaca gttatccgca
     4681 tcagttctca ggaggtcagc gtcaacggat cgtgatcgcg attgcactgg tatgttatcc
     4741 ggaaatactg attgctgatg aacctacaac agcgcttgat gtgactattc aagcacagat
     4801 tttggagctg ctgaaagaaa ttcagacaaa aatcgataca tcaattatct ttatcactca
     4861 tgaccttggt gtagtagcga atgtcgctga tcgtgtcgct gtcatgtatg gaggaagaat
     4921 cgttgaagtt ggaacatcgg aagaaatttt ctacaacccg caacatccct atacttgggg
     4981 actgcttggt tcaatgccga cattagatag tgcgaacgat cgattgtatg ctattcctgg
     5041 ttctccaccg gatttattga acccgccaaa aggagacgca ttctatccaa gaaatgaatt
     5101 cgcgatgaaa atcgatgcag aacaagaacc accatttttt gaactatcaa aaacacacaa
     5161 ggcagcaact tggctacttg ctccgcaggc acccaaagtg acaccgccag aagaaatcca
     5221 acgtcgctgg gctatcttta aagagaaaca aaaacatact tacggtcaag gaggaattgc
     5281 tgatgccaaa taaactactt gaagttaaag gattgaaaca gtatttcaat gtaggaaaaa
     5341 agaatgaagt acatgctgta aatgacatca gctttcacat ttatgaagga gaaacattcg
     5401 gtttagttgg agaatctgga agcggaaaat ccactactgg ccgtacgatc attcgcttga
     5461 atgaacctac tgatggtgag atattattcg acggtcaaga tataacgaag atcaaagata
     5521 aaaaaggttt gacgaaattc cggcatgatg ttcagatgat ctttcaagat ccatacgctt
     5581 cgttgaatcc tcgtatgaaa gtcagagata tcattgccga aggaattgat gttaatggac
     5641 tagctaaatc atctgaagag cgtgcaaaaa aagtcgatga tctattgaga actgttggat
     5701 tgaatccaag ccacggtact cgttatcctc atgaattttc aggtgggcaa cgccagcgta
     5761 ttggtattgc acgagcattg gcagtaaaac cgcgttttat catttgtgat gaaccaattt
     5821 ctgcactaga tgtgtcgatc caagcccaag tggtcaattt gttgcaagat ctccaaaaag
     5881 aacatcaatt gacctatcta tttattgcac atgatttgtc gatggttaaa catattagtg
     5941 accgaatcgg tgtaatgcat aacggcttgt tattagagat gggaacaagt gatgaaatct
     6001 ataatcacgg tgttcatcct tatacagaaa gtttgttatc tgctattcct ctaccagatc
     6061 cagaccatga gagaaaacgc agaagaatca aatatcaacc tgaaccagat gacggacaag
     6121 tacgtcaact tcgtgaaatc gcaccagaac attttgtcta tgcaactgag caggaagtcc
     6181 catattacgc aaaaaaacta aaacgacaaa aggaatctct tctggttgct aactaggagg
     6241 gacatagatg ctaaaagctt ttaaattccg tatgtatccg acagaagaac aaaaacagca
     6301 attgattcgt acttttggat gtgctcgatt tacctataac catttgctga aaaagcggca
     6361 gaaaagttgg caacaaacag gagtagccaa tttttcgctc acacccgcta cattaaaaaa
     6421 agaataccca ttcttaaaag aagtagacag tcttgcttta gcaaatgcac agcttaattt
     6481 ggatcgggct tttcgcaatt atttcaaggg acgtgccagc ttcccaaagc ttaaaacgaa
     6541 aaaaagtatg tggcaatctt atacgaccaa caaccaaacc cgtacgatct atctggaaaa
     6601 tggctactta aagttaccaa aacaaaaaga actaatcaag ataaatcagc atcggccagt
     6661 tgaaggatcg attcgctccg ctacgatttc tgctcgatat aatgaagaat tttatgtcgc
     6721 actactttgt gatgtttcgc cagtaaaaaa agaatcttta gctaaatgga tcgggatcgc
     6781 ctatcatccg aagacattga tccaaacatc tcgacctcta gaagtaactt taccaaaatt
     6841 tcaccagaca gaagaaaagc tgcaacatgc acagcggaaa ttgaatgtga aagtccgctc
     6901 cgctcaccat agaaagatca ggttagatca ggcaagcaat tatcagaaac aaaagagaaa
     6961 agtaatggat ctttatctga aacaaaagaa ccagcgagaa gattatcttg aacaattatc
     7021 tgggaaattg gtcaaacaat atgactactt atttgtagaa tcttttccaa aagaagaggc
     7081 acatgctgat ttttctattc acgactggca taaattaata acaaaattgc gttataagtc
     7141 ccaatggtat aataaaaaat ttcttttgat caataccgac ggagcagaag aaagcaattc
     7201 cgtaagaaag agccaagtag tagaaaaaat gggcagacat tcggttatca agggataaaa
     7261 aatgaatgaa actgatagga gcgttagggt tagccttgtt aataaaaagt cttaatgact
     7321 tttgtttcta ggaatttttc ttttgaagaa aagctcacta attatgacaa accaataaaa
     7381 caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt attcagaaag
     7441 tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg
     7501 ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat ggtgccccgg
     7561 cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac cgatctcatc
     7621 gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat aatcgatccc
     7681 taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg
     7741 tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt tcaagatgat
     7801 ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga ctaaatcaag
     7861 cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa
     7921 ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa taaaggctaa
     7981 gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga atgtatgcag
     8041 ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt ctcttaagac
     8101 gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc atgaatttcg
     8161 ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt ctttgttata
     8221 gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac gttcctgttt
     8281 ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata atttttcttc
     8341 ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag tactttttac
     8401 cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct aggttatcat
     8461 ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca gataaaacgg
     8521 caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga tatatacgtc
     8581 aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc catggaggtt
     8641 aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta atgaaagaaa
     8701 agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag tcccttacta
     8761 agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg ttttttagta
     8821 gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt aaaggataga
     8881 ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa gctaacagca
     8941 tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata aaggaggaag
     9001 agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta tccaagcggc
     9061 aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc tcgttataaa
     9121 cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg tcaaaaaatc
     9181 gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa aatggcggct
     9241 gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat tcgcacgaca
     9301 gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga acagggagat
     9361 atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt ctttacagaa
     9421 acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg taaagcgcca
     9481 agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat gagcaaatac
     9541 gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc agaatctcgt
     9601 aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc agtttcacga
     9661 acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt gtatgtatgg
     9721 atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga tgaaagtcta
     9781 ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt tcgctttcat
     9841 acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa aaaagtattt
     9901 gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt
     9961 tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta tttgcttcgt
    10021 gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc ccgtgtcaat
    10081 tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat gatcaacaaa
    10141 tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga tagtgaactg
    10201 tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa aatggaattc
    10261 aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata cattgatgaa
    10321 acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg
    10381 atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat catgacagaa
    10441 acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa tcttgaagga
    10501 ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac accaatcttc
    10561 ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc tgaaggtact
    10621 caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa
    10681 gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc agaagtcatc
    10741 aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga tgctggagac
    10801 agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc aagcgaattg
    10861 atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc
    10921 agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt aatcgaagca
    10981 ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg tatgacacta
    11041 gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat tccttcttat
    11101 tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa tcctagaaaa
    11161 agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg aatcgtaaaa
    11221 acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca tctggtactc
    11281 ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac atctatattt
    11341 atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg ttcaattagt
    11401 ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa acaatcatcg gctttgactt
    11461 cgatgattgt tttctttatt ttgtaaaaat cctatcactt gattttatgg taacctttta
    11521 tttttgtctt caaacggctt ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga
    11581 cataccagaa aatcgtacgt ccgataccat aatgttgata gagctttgtc aacatcacgt
    11641 gtaacaagat caaccctaaa agcaaggagc cagctataaa ccaatgatta tcccaatgca
    11701 tgacttcact aagtgccatc actccaaaag gatagaaata aaaatagatc ccgactaagg
    11761 tcatccgcca gttctttgta aatgttccga tattcaataa aaagaaaatc gctgaaagca
    11821 aaataccgat aggcggaaaa agcccaacca aaaaggaata caacatcccc cacaagatca
    11881 tcacaggacc tttgatcaat gcaacgattg cgatgaacaa agtcagcaat attagttttt
    11941 gtgataacgt tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa tgccaatggg
    12001 actgctgttg ttctttacgg gaaaaatcga cccggttgga taaataatct gctaatttcg
    12061 gataagaacg cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta ggtttagtat
    12121 acgaaaaata aaaaagaaat acatccgtct taagagggag tttttcactg aaagtggaga
    12181 aaaaagaatg gtaaattaaa atattctcag ttatactgaa aattatacca aatttcaaag
    12241 aagaaagagg atgggaatga aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt
    12301 agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac
    12361 tgctagtact cagtctacaa gtttgacatt gctagcttgg cttttgggtg gtttcttaac
    12421 gatctgtgct ggattgacag tggctgaatt agctacagca atcccggaaa ctggtggagc
    12481 tgtaaaatat attgaagctg cgtatggaaa attgcccagc tttttattag gatgggcgca
    12541 aagcttgatt tactttcctg ctaatattgc tgcattatcg attatctttg cgacacaatt
    12601 gacgaactta ttacaattat ctaccgatta tcttttgttg attgcagtga tcaccgctgt
    12661 ttctgtgaca gggttgaatt tgttagggac aaaagtcggt gcttctgtac aatcagtgac
    12721 gttgattgtt aaattaatac caattgctgt gattgtcata tggggattat tgaccccagg
    12781 acaaggaact gtccagttat ttccaatcga agctggaaaa gatgttactt ttgtcgaagg
    12841 attgagcagt gcgttgttag caacgttgtt tgcttatgac ggctggttag gtgtcggtgc
    12901 aatggcaggg gaaatgaaac ggccggaaaa agatttgcct aaagcaatta ttcttggttt
    12961 gagttttgta acagtcgttt atctattgat caattttgtt tttttgaaaa ctttgccgat
    13021 tgatcatctt gccggtaatt tgaatgcagc ttctgaagca tcagacgtca tttttggagg
    13081 aatcggtgga aagctggtca ctattgggat cttgatctcg gtatacggtg cattgaacgg
    13141 ttatacacta actgggatcc gcgtgcctta tgcgatggct ttagaagacg agctaccttt
    13201 cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt
    13261 ccagttagca gtagcttgta ttatgatgag ccttggttct ttcgactttt taacagacat
    13321 gttgattttt gtgatgtggc tattcagttt actgattttt atcggtgtct ttattttaag
    13381 aaaaaaagcg ccggaattgc cacgtccata taaagtacca ctttatccaa tcgttccgat
    13441 tattgcgatt ctcggtgcta ttttcatttt aggtatgacg atgatgacgc aaacgaaatt
    13501 agcattgatc ggtataggtg ttaccttgat aggtatccca gtctattatc aaaaaaagaa
    13561 aaaacgctca gaagagtaaa atatttcaac aacagacaga aaaagtacga actaggtcct
    13621 ctagatcttc gtgctttttc tatttcaata aaacaagttc ttctattttg atttttgcta
    13681 cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa aggagagata aacatgatat
    13741 ttgattctca tacccattta aatgctgaac agttcaatga agatattcca gaaacgattc
    13801 agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga
    13861 ttgaaaagtc tttgcaatta agccatgact atccaaatat atacagtatt atcggctggc
    13921 atccgacaga ggctggcagt tatacgaaag aaatcgaaaa taaattacaa gaacaattga
    13981 ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga ttatcattgg atggaagacc
    14041 cgaaagaagt ccaagataaa gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc
    14101 taccaatcag tattcatact agagaagcac ttgaagacac gtatcaaatc ctaaaagaag
    14161 aagatgttcg agatatcggt ggaatcatgc atagtttcag tggtgattat gaatgggcaa
    14221 aacgatttct tgacttaggg atgcatattt catttagcgg tgtcgtcact ttcaaaaaag
    14281 cattagacgt ccaagaggct gcagcaaatg taccaatcga acgattactt gtggaaacag
    14341 atgcccctta tctggcacca gttcctttta gaggaaagcg aaatgaacca ggctatactc
    14401 gttatacagt agaaaagatt gctgaacttc ggaatcttcc gacagaagaa gtggctgccc
    14461 aaacatggac aaatgcgcac cgattatttg gtctaagcga acatggataa gatgaagatc
    14521 caagagatta tagtcgttga aggaaaagac gatacaagaa ggctgcggga agtagttgat
    14581 gcagatacga tagaaacaat agggtctgca atcaatgaag agatcctgat gcaaattgaa
    14641 catgcccaag agactcgtgg agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa
    14701 ataagaaaaa tcattatgga agctgttcct caggcaaaac atgctttttt gccgcgcaca
    14761 caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt
    14821 ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt ttctgaaatc
    14881 actagacaag accttttaga ctatggttta atagctgggg cacaagcaaa agaaaaacga
    14941 gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt
    15001 ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa
    15061 caacatgagt gaatatcgtg aaattgcgac accctctaga acaaaagaga ttttattgaa
    15121 acatggtttt tcatttaaaa aaagtttagg acaaaatttt ctgactgaac caaatatttt
    15181 aaggaaaatc gtagaaactg cgcaaataga tgaacatacg aacgtgatcg aagttggacc
    15241 aggaatcggt gcattgacgg aacaattagc caagcatgca aaacaagtcg tagcatttga
    15301 gatcgatgat cgtttgatcc ctgtattggc tgatacgatg caaccctatg acaatgtgac
    15361 tattgtacat caagatattt taaagaccga tttgtcaaca gctgttagag aaacatttca
    15421 tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac tatattacga caccgatcat
    15481 gatgcatttt cttgaatcag atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga
    15541 agtagctgat cgtatctctg ctgaaccagg aacgaaagct tatggttcat tatcgattgc
    15601 tgttcaatat tatatggaag ccagtttagc attcatcgta ccaaaaacag tatttgtccc
    15661 tcagccaaat gtagattcag cgattttgaa actgacaaga agagatatac cggcagtcga
    15721 agtgacagac gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa
    15781 aacgctttgg aataacttgc agcattcgta tggcaaagat gaccagacaa aagcatggtt
    15841 agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt
    15901 acaagaattc gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt
    15961 gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    16021 agttcctcat gtttttcgtt tgtttgggct tattaccgca gatgcaagct tttgaacacc
    16081 gtttaagaaa gaggttgtgg gcctgccgtt taactctaag tcacaaggaa caatttcaaa
    16141 aaacagcttc tcatgttttt gagttagttg gtcttattac ttgatgcaag cttttgacca
    16201 ctattatgcc gaacaaggag atgtgacaca aattttgtca catctcctat ttttgtttct
    16261 tgatcaaaaa attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat
    16321 atgtgtcttg acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac
    16381 atattaggag agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt
    16441 attgattggg atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc
    16501 ttcctttact ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt
    16561 tgcagtggct gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat
    16621 cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa
    16681 gcatccgagt atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg
    16741 gttcaatttt gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt
    16801 ttactttatt ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt
    16861 catcttgatg gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt
    16921 tatagcagga acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga
    16981 aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct
    17041 caaaaaaaaa actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt
    17101 cctaaaaaga gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa
    17161 gtttgatgac ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa
    17221 ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag
    17281 tagaaccagt ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt
    17341 ctgatttcac tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt
    17401 ctggacgaat accgaagata acttcttttc cttcataacc tttttcaact aatactttgt
    17461 tttttccttc aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca
    17521 atgtcacatt aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga
    17581 cgggtgtatc ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga
    17641 taacgattcg gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg
    17701 ttgtttcaag acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag
    17761 catctaaatt tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc
    17821 gtcccaatgc cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt
    17881 attctgtcag acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat
    17941 catatttacg taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca
    18001 atgcgtagtt ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga
    18061 cttttccgcc gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca
    18121 aagtagttga ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga
    18181 ttgatagatt aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt
    18241 ttttcagagc catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta
    18301 tttaaagtat aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc
    18361 ttttttttgt caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg
    18421 atacactgga gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg
    18481 ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa
    18541 caatattgga aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag
    18601 caaccggctt gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg
    18661 gagccatgat ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg
    18721 cacagcccca tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc
    18781 cgttagctac gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc
    18841 tagattttcg aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa
    18901 gcgtatggaa aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact
    18961 gataatatgc gatataatac gaacgtattg gataatataa acaataatat tagtttttcg
    19021 tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat
    19081 acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag
    19141 gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta
    19201 aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc
    19261 ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt
    19321 aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg
    19381 tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca
    19441 ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc
    19501 tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc
    19561 atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc
    19621 tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt
    19681 gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc
    19741 cacgctaaat aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc
    19801 tgtgaattgt gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg
    19861 ggtgcccatt tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat
    19921 tgtggacagg cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc
    19981 ttgttggatt tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa
    20041 cataggggga ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga
    20101 tatctttaac ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa
    20161 ttcatacata taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt
    20221 cccaaaacga agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat
    20281 tccaaaactt ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt
    20341 aattgttcct tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc
    20401 ggcccctgtt tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc
    20461 ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc
    20521 tttgatattt ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg
    20581 atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat
    20641 tccagaaagt tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat
    20701 cacaccaaca gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat
    20761 cgataaataa gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa
    20821 ttgtgcccgt tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact
    20881 agcttcttct tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa
    20941 gcccgagata tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc
    21001 aacgatccaa ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag
    21061 tggacttaga cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa
    21121 ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact
    21181 gacgatattt ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc
    21241 aaatgcatct tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg
    21301 gatgagccat tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg
    21361 gaacagtcga ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat
    21421 ttgtaacgtc atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca
    21481 aatgtctttt gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa
    21541 tttttttact ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct
    21601 gattgagcaa gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact
    21661 aagatatgat gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg
    21721 ccaagataag tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt
    21781 aaagagtcat ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt
    21841 cgctgttttt gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca
    21901 tttggaagct gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct
    21961 ttttctgtgg cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt
    22021 acagaagttt ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga
    22081 acatccattc cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat
    22141 tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc
    22201 ttcagctgaa atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca
    22261 acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct
    22321 tcaagaatcg aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca
    22381 cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata
    22441 taattgatca aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg
    22501 ccaactgtca gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat
    22561 agggcagaga tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg
    22621 aacttctctg tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga
    22681 ttctgactaa cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga
    22741 acggtttttt tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa
    22801 aacagaccta cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac
    22861 ggtgctctga tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg
    22921 tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa
    22981 gtattgattt ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact
    23041 gaagcatagt attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc
    23101 cataagccca tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag
    23161 gaagccatca ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg
    23221 cccaaaatga tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc
    23281 ctcctattat ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga
    23341 aatttagaat gaaaagagaa caagcctttt cataaatgaa agttttatta tgaagaagct
    23401 ttagaaattg aatagactat tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc
    23461 tt
//
LOCUS       DBKOIJ010000030        22382 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000030 DBKOIJ010000000
VERSION     DBKOIJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.030"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..662)
                     /locus_tag="ACV5NJ_000902"
     CDS             complement(<1..662)
                     /locus_tag="ACV5NJ_000902"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HHY6485016.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LE"
     gene            complement(675..1391)
                     /locus_tag="ACV5NJ_000903"
     CDS             complement(675..1391)
                     /locus_tag="ACV5NJ_000903"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HHY6485017.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            1721..2329
                     /locus_tag="ACV5NJ_000904"
     CDS             1721..2329
                     /locus_tag="ACV5NJ_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HHY6485018.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            2654..2977
                     /locus_tag="ACV5NJ_000905"
     CDS             2654..2977
                     /locus_tag="ACV5NJ_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HHY6485019.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            3159..4529
                     /locus_tag="ACV5NJ_000906"
     CDS             3159..4529
                     /locus_tag="ACV5NJ_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HHY6485020.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(4659..5960)
                     /locus_tag="ACV5NJ_000907"
     CDS             complement(4659..5960)
                     /locus_tag="ACV5NJ_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HHY6485021.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(6314..8305)
                     /locus_tag="ACV5NJ_000908"
     CDS             complement(6314..8305)
                     /locus_tag="ACV5NJ_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HHY6485022.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(8449..8715)
                     /locus_tag="ACV5NJ_000909"
     CDS             complement(8449..8715)
                     /locus_tag="ACV5NJ_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HHY6485023.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(9225..11351)
                     /gene="pnp"
                     /locus_tag="ACV5NJ_000910"
     CDS             complement(9225..11351)
                     /gene="pnp"
                     /locus_tag="ACV5NJ_000910"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HHY6485024.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(11539..11808)
                     /gene="rpsO"
                     /locus_tag="ACV5NJ_000911"
     CDS             complement(11539..11808)
                     /gene="rpsO"
                     /locus_tag="ACV5NJ_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HHY6485025.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            12059..12622
                     /gene="def"
                     /locus_tag="ACV5NJ_000912"
     CDS             12059..12622
                     /gene="def"
                     /locus_tag="ACV5NJ_000912"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HHY6485026.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(12875..13396)
                     /locus_tag="ACV5NJ_000913"
     CDS             complement(12875..13396)
                     /locus_tag="ACV5NJ_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HHY6485027.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(13399..14241)
                     /locus_tag="ACV5NJ_000914"
     CDS             complement(13399..14241)
                     /locus_tag="ACV5NJ_000914"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HHY6485028.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(14234..14959)
                     /locus_tag="ACV5NJ_000915"
     CDS             complement(14234..14959)
                     /locus_tag="ACV5NJ_000915"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HHY6485029.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15135..15431
                     /locus_tag="ACV5NJ_000916"
     CDS             15135..15431
                     /locus_tag="ACV5NJ_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485030.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      15687..15861
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            15999..17591
                     /locus_tag="ACV5NJ_000917"
     CDS             15999..17591
                     /locus_tag="ACV5NJ_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHY6485031.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            17604..18344
                     /locus_tag="ACV5NJ_000918"
     CDS             17604..18344
                     /locus_tag="ACV5NJ_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHY6485032.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            18512..19861
                     /locus_tag="ACV5NJ_000919"
     CDS             18512..19861
                     /locus_tag="ACV5NJ_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHY6485033.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(19989..20579)
                     /locus_tag="ACV5NJ_000920"
     CDS             complement(19989..20579)
                     /locus_tag="ACV5NJ_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HHY6485034.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            20726..22078
                     /gene="brnQ"
                     /locus_tag="ACV5NJ_000921"
     CDS             20726..22078
                     /gene="brnQ"
                     /locus_tag="ACV5NJ_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HHY6485035.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6401 a         4504 c         4290 g         7187 t
ORIGIN      
        1 tcctctaatg aatcgattcg ataagtaggc tggatttctt cgatttctgg taatttattt
       61 ctattgaacc aaatcgtatc gatccctgcc atatttcctc ctttgatatc agaagtcaaa
      121 gaatcaccaa tgatcacagt ttgcttttta tcaaaattag gaatgtggga aaagacatga
      181 tcaaaaaatt ctttcatcgg cttttgcgct ccgacagctt ctgagacaaa gatatccttg
      241 aagtaatcaa gcattcctgc atctgttaat cgccgatgtt gcgtgtgcga cacaccgttc
      301 gtcacaatat aaagatcagc atgcggatgg acctttttca ggatttctag acttcctgga
      361 acaagatcat gaccttggtt caagtagtgg cggtagcgtt tttccatttg atcactatct
      421 actgttttcc caaattgctg gaaaagtaac ccaaaacgac tgcctattat ttcttctcgt
      481 gatagtctgc ctttttcaaa ctcctgccat aaatgatgat tcaaagcctt atagctggtt
      541 tcgatttctg gtgtcaatgt gattccttgt tctgcaaaca aagcacttaa agcattggct
      601 tcagttaatt gaaaatctaa caatgtatca tctacatcaa acaataaagt tttatatttc
      661 atctttttga cctcctattt aaaataaata cttaactttt tcatctctgc taatgtcaac
      721 gatcgatact cgcctactcc tagctgttca tctaattcca atggtcccat gcttagccgt
      781 ttcaatgcgg tcactttctt tccttgggct aagaacatct ttttgacttg atggaatttt
      841 ccttcttgga tcgtcaataa aaccgtactt tggtgcttgt ctgctttgag tatttttaaa
      901 tcagccggct tacattgata cccatcagcg aatacgattc cattttcaaa tgctgttacg
      961 tctgccggtg tcaccctttc gttcacaact acttgataag tctttgaaac ctttttttct
     1021 ggaagtagca tttcatatcc caattgtcca ttcgaagtga tcagcaatag tcctgttgta
     1081 tctctatcta gtcttcccac tggatataat ggttggaaac gatcggcact tgcaatcaga
     1141 tcgattactg tcgtcttttc tggatctgat acagctgtaa cagctttttc aggtttattg
     1201 agaagataat aggactcatt tgtttctaag tgttcacctg ctacttcgat ttgatgcagt
     1261 aaactatcta catttcgatt ttgtcgaaat tcgactgtgc catctatcat cacttgcttc
     1321 aaagcaaaca gccgcttcat ttccttgcgt gtcgtttcta atttttcttc tattaattta
     1381 tctaaacgca tgctgtttcc tttcttatta ctactaatta attatacctt gaatattctt
     1441 gcttgcaaat acatcatcat agttattcct tgacttaatt aacagttcac gttacggtta
     1501 aatagtaaag tgaattggat ttctcataca cagcgtaatg acctggtgga gaaaagttga
     1561 atcctgactt tctaagggga gatgcgcgca tctctccttt tgcgcttttt taagcgccct
     1621 gccttcgatg atgtgcacga gattagcctt tagaattcac ttaaataaaa tctgtccgtt
     1681 accaaagcag acagtcacat tttaaggagg aattttggtt atgtcagtat cattagaagt
     1741 aagtaaaaga gaagtacgtc cccgttcatt aagaaacaaa cttcgtcacg aagggaaagt
     1801 tccagctgtt gtttatgggt ataaagtaga aagtacccct atctcattcg atggtttgac
     1861 actaagcaaa ttgttgcgtg aacatggagc gaacacagtc atcacgatgg acatcgatgg
     1921 acaaaaagtc aatacattgg ttcacaagat ccaaacgaac acttttacca atcatttcga
     1981 acatgcagaa tttctatctg taaacatgtc ggaagaaaca gaagtcgaaa cagaagtcgt
     2041 tcttgttggc gaatcagtgg gtgctaaatc tggtggagtg ttagctcaaa acttgtatac
     2101 agtagtcgta tctgctacac cagacaaatt gcctgaacga attgaagttg acgtgacaaa
     2161 cttggaatta ggtgattcta ttacagtagc cgatcttcca aaaaacgacg attacaaatt
     2221 cgtgaccgac agtgaagagc aaatcgctgc tgtagtcgct caaagtgaag aagaagaaag
     2281 cacaggtgaa ggtggcgaac cagaagtgat cggttcaaca gaagaataat actgaaacaa
     2341 tcaggtggtg ttagaagcgg cgggtcagca gcttttaaca cgcctttttt atttctaatc
     2401 gtttattttc atatggggct tccacttttg taaatggctt tacaaacgaa aaatcctaat
     2461 ttaccaagct tttcctcgca tacttttatc gtacctttat aaaaatttat actttgaaaa
     2521 aaagtattca attattgttg catttttcct gattctttat tagaatgaac cctcgttgaa
     2581 gtaagtattt ctagtttatc ctaggattaa ttataaaaat aaaacattcg ttgattcgaa
     2641 ggaggttaaa cagatgtttg aaagttttga tagcagccgc aaccgttacg cttcagttgg
     2701 tgtcgtatca agtttgccag atgaattgat tgacagtata tggtttatta ttgacctaga
     2761 tcttaaggga gtcattccat tagaaaatat attaaccttt gatttgatcg aaaatcgtgg
     2821 aaaagtgacg atgcattttt ctcaagcagg aagcaatgtg gaaatggata ttgatttgcc
     2881 gtttgcctat tcgaaccaat ttcctgctca agtatacgcc tatgatgacg gtacgagaga
     2941 aacgatcctt ttaccaagtg agattagaca atattaaggt gatagataaa cgtggatgtc
     3001 ccttatttta cagaccaggc caagttgttg attctttgac tcaattcttg gcttcttttt
     3061 atggagtttc ataaattatc attgatttct aattggtatt ctcaggataa acgctatata
     3121 ctactaacta aattttaaag atataaggag tttaaaagat gcgcgattta acaaaaggaa
     3181 gccccgctaa actgattttg atgttcactg ttcctttact gatcggaaat gtttttcagc
     3241 agttctacaa tatgatcgat atgatcatcg tgggtcagac gctaggtaaa aatgcgctgg
     3301 cagcggttgg tgcaactgga agcctgacat ttttgatcat tggctttgcc caaggtttaa
     3361 cagctggttt agccattatt accgcacaac gttacggtgc aaaagattat cggggtctga
     3421 agaaaagttt tgctgctagt gttgtcatta gtcttgtcgt cactgtgatt ttaactgtcc
     3481 taagtctcct gtttattcgt ccgatgcttc aattgatgca aactcctcct gaaatacttg
     3541 atcaagcaca aacatttatt tcaattattt tattagggat ctttgcatca gtcagtttca
     3601 atttgctttc taatgtcatt cgcgcacttg gtgacagccg cacaccacta ttctttctga
     3661 tcattgcggt catcatcaac gtcgtacttg atttgatctt tattatctat tttggcatgg
     3721 gtgtggaagg tgcagctatt gctacagtaa tcgcacaagt ttcttccagc gtactgtgtc
     3781 tagtgtatat caagaaaaaa atgcctttgt tacagttacg caaaaaagat ttcaaatttg
     3841 aaaaagaaga gtttgctgta cacctaaatg ctgctttgcc aatggcgttt caatcttcga
     3901 tcatcgctgt tggtgctatc gtgttacaag cagcactaaa tagtctaggg acagatgtcg
     3961 tagctgctca agccgctgct ggtcgtatcg accaatttgc aaatcagccg atgatgtcat
     4021 ttggtattgc aatggcaact ttcactgcac aaaactatgg tgcgaaagaa tatggccgaa
     4081 tcttgcaagg ggtacgtcaa actttactga tgagtgtcgg atttagtctc ttagcaggtg
     4141 ctatcgtcat tttcttcggt catttctttg ttcaattgtt tgttagccca tcagaaacac
     4201 gtgtatttga attagcacaa acctacttca atatcaatgg gtctctttac tggatcttag
     4261 cagtcctgtt cattttacgc tacacacttc aagggcttgg acaaagcaag atccctacga
     4321 tcgccggtat gatggaattg cttatgcgtt cttttgcagc tatcgttctt acaggaatgt
     4381 ggggataccc aggagcagca gcagcttctc ctttagcttg gatcggttct gttgccgtgt
     4441 tgctttactc ttatatgcgt tcgatgaaac aattgaaaca catgggtgaa gaggaacatt
     4501 tcacattaga acatgcagaa gcaagatagc cttgattaaa agaaatcgtt gcggctaaaa
     4561 atgaaaactt ttattgaaaa tatcttcttc gtttaccgaa aacaggtttt ttagataaca
     4621 aaaaggctgt gacaaaaaat gtcacagcct tttttctttt attctgtttt tctttccgtg
     4681 gactctactt tgaaataatc gatatgtgca tgctggtatt ctaataccgt gaaacggaat
     4741 gatgcaatct caatgattgt tcctgctttt atttctttat atcggctaga agcaaagccc
     4801 gcaagtgtag taaaattcgt ttcttcaaac tctggaatct ctacatgaaa ataacgctgg
     4861 aaatcatcta atgttagttt gccggagatg atataagagc catctgactg ttccgctatc
     4921 aatgcttcgt ccaaatctgt ctcatctcgg atttccccga cgatttcttc taaaatatct
     4981 tcaatcgtca ctaagccgct tgtccctccg tattcatctt ttaagatagc aatatgctta
     5041 cccttttttc gcatgatcgt caataatttt tttataggga tcgtttcagt tgttacgatc
     5101 ggctcttcaa tcaactgttc tactgtcagc tggtcatctt tataagattg cttgatcaaa
     5161 tctggcaagg tgacataacc taaaacattg tcttttgatt ctaagataac cggatagcga
     5221 gtatgtcctt gttcaatcga agcttttatc gcttctttga cggtttctga agcttcgaat
     5281 acttgcatgg acgtacggtc tacttgtata tcttttgcca aggtatcatc aaattcaaac
     5341 acgttttcca aataataata ttcttctttg gtcaactctc ctttttcata agaattcaat
     5401 gcgactaatg tcagctcttc ttgagacaat gtctcttctc cttcagatac gagacgcaca
     5461 cctaatagct tccctagccc tgcagctgaa tgattcaata cccaaataaa aggaaacatt
     5521 actttataaa aataatgaag tggtttaact acgaacaaga cgaccttctc cgtattgacg
     5581 atactatagc tcttaggaag cagttcgcca ataaccacat cgatatacgt aactaaaata
     5641 aatcccagcg ccacagagat tgtactgcta atagccgaac taaaaggtag tttaccgatc
     5701 actggttcta gtaaagccgc gacagatcct tcaccgaccc acccgatgat caaaccggtt
     5761 agcgtaattc ctaattgact agctgataaa tagttatcta aatggtgtgt aacatgaaga
     5821 gctagcttgg cgttctttac attttccttt tctagttgtt ccaatcttga tcgtctgatt
     5881 ttcactaaag caaattcact tgctacaaaa aatgaagtaa ttgcaatcat caaaaataaa
     5941 ataatgaaat tgataaccat attctcccgc ctttctcttt ttctattgta ccctttttct
     6001 acaaaaagat tgaaaataat gcgtattcat atttaggaat aaaaaaggag atgcggcatt
     6061 tttcagccac atctccttat cacttcaaaa tcattgcgtt acttgttgct aaacagcttt
     6121 ttcatgacct ctgataaaaa cggtattcaa aagcttgcat ctacggcatt aagcccaaac
     6181 agatgaaaaa catgagaagc tgtttttatc tgctcgttct tactgcttgt tgcagtacag
     6241 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     6301 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     6361 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     6421 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     6481 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     6541 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     6601 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     6661 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     6721 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     6781 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     6841 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     6901 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     6961 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     7021 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     7081 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     7141 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     7201 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     7261 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     7321 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     7381 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     7441 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     7501 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     7561 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     7621 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     7681 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     7741 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     7801 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     7861 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     7921 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     7981 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     8041 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     8101 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     8161 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     8221 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagtc attgatatct
     8281 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     8341 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     8401 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     8461 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     8521 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     8581 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     8641 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     8701 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     8761 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     8821 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     8881 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
     8941 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
     9001 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa ataaaaacag ctcctaatgt
     9061 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
     9121 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
     9181 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
     9241 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
     9301 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
     9361 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
     9421 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
     9481 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
     9541 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
     9601 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
     9661 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
     9721 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
     9781 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
     9841 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
     9901 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
     9961 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    10021 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    10081 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    10141 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    10201 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    10261 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    10321 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    10381 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    10441 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    10501 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    10561 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    10621 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    10681 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    10741 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    10801 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    10861 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    10921 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    10981 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    11041 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    11101 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    11161 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    11221 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    11281 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    11341 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    11401 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    11461 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    11521 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    11581 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    11641 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    11701 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    11761 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    11821 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    11881 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    11941 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    12001 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    12061 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    12121 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    12181 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    12241 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    12301 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    12361 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    12421 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    12481 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    12541 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    12601 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    12661 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    12721 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    12781 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    12841 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    12901 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    12961 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    13021 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    13081 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    13141 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    13201 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    13261 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    13321 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    13381 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    13441 catcaaatca acatacgtat ctgttattgt ttccttatat cccttatttt tcagcagact
    13501 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    13561 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaatcaa
    13621 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    13681 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    13741 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    13801 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    13861 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    13921 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    13981 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    14041 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    14101 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    14161 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    14221 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    14281 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    14341 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    14401 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    14461 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    14521 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    14581 gacaatgaca ttgatacaaa cttccatgaa aattgacgag ttgcgggctt ttagcctttg
    14641 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    14701 ccatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    14761 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    14821 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    14881 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    14941 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    15001 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgaacaa
    15061 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    15121 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    15181 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    15241 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    15301 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    15361 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    15421 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    15481 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    15541 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    15601 caaaaaacag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    15661 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    15721 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    15781 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    15841 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    15901 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    15961 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    16021 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    16081 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    16141 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    16201 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    16261 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    16321 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    16381 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    16441 aaagtttagc agatctttct ggagcaaaga ttactgctca gttaaataca ttccactata
    16501 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    16561 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    16621 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    16681 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    16741 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    16801 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    16861 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    16921 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    16981 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    17041 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    17101 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    17161 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    17221 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    17281 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    17341 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    17401 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    17461 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    17521 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    17581 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    17641 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    17701 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    17761 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    17821 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    17881 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    17941 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    18001 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    18061 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    18121 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    18181 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    18241 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    18301 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    18361 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    18421 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actcttttaa tatggaagta
    18481 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    18541 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    18601 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    18661 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    18721 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    18781 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    18841 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    18901 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    18961 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    19021 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    19081 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    19141 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    19201 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    19261 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    19321 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    19381 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    19441 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    19501 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    19561 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    19621 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    19681 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    19741 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    19801 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    19861 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    19921 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gttaaaagct tctgtcccag
    19981 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    20041 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    20101 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    20161 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    20221 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    20281 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    20341 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    20401 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    20461 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaaga
    20521 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    20581 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataaccg
    20641 tcgttttata taggaaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    20701 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    20761 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    20821 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    20881 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    20941 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    21001 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    21061 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    21121 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    21181 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    21241 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    21301 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    21361 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    21421 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    21481 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    21541 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    21601 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    21661 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    21721 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    21781 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    21841 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    21901 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    21961 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    22021 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    22081 aaagaggttg tgaaagagac gttcagcctt attgccgata ggctgtctct tgaacactgt
    22141 ttattgagag gtcgtgaaaa agccgctcag tttcgagtat taagaaatat tttttgaaaa
    22201 tggcttctca catttttgca taaatttgac ttaataccga gaaactggct tttgaacacc
    22261 gtttattgag cgtctataaa ctgacctgag tattagagaa acaaaacggc aaagtagctc
    22321 catatacttt gccgttttgt tttgaactaa taccgaagag aagctgatca aacaacattt
    22381 ct
//
LOCUS       DBKOIJ010000031        22041 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000031 DBKOIJ010000000
VERSION     DBKOIJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.031"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..288
                     /locus_tag="ACV5NJ_000922"
     CDS             <1..288
                     /locus_tag="ACV5NJ_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485036.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACV5NJ_000923"
     CDS             347..787
                     /locus_tag="ACV5NJ_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485037.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACV5NJ_000924"
     CDS             799..1467
                     /locus_tag="ACV5NJ_000924"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HHY6485038.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACV5NJ_000925"
     CDS             1531..2154
                     /locus_tag="ACV5NJ_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HHY6485039.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACV5NJ_000926"
     CDS             2210..2596
                     /locus_tag="ACV5NJ_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HHY6485040.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACV5NJ_000927"
     CDS             2809..4200
                     /locus_tag="ACV5NJ_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HHY6485041.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACV5NJ_000928"
     CDS             4811..5179
                     /locus_tag="ACV5NJ_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HHY6485042.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACV5NJ_000929"
     CDS             5359..6300
                     /locus_tag="ACV5NJ_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HHY6485043.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACV5NJ_000930"
     CDS             6369..7100
                     /locus_tag="ACV5NJ_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HHY6485044.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACV5NJ_000931"
     CDS             7244..9424
                     /locus_tag="ACV5NJ_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HHY6485045.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACV5NJ_000932"
     CDS             9444..9926
                     /locus_tag="ACV5NJ_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HHY6485046.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACV5NJ_000933"
     CDS             9942..10703
                     /locus_tag="ACV5NJ_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HHY6485047.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACV5NJ_000934"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACV5NJ_000934"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HHY6485048.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACV5NJ_000935"
     CDS             12475..12960
                     /locus_tag="ACV5NJ_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HHY6485049.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACV5NJ_000936"
     CDS             12953..13636
                     /locus_tag="ACV5NJ_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HHY6485050.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACV5NJ_000937"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACV5NJ_000937"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HHY6485051.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACV5NJ_000938"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACV5NJ_000938"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HHY6485052.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACV5NJ_000939"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACV5NJ_000939"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HHY6485053.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACV5NJ_000940"
     CDS             17933..18265
                     /locus_tag="ACV5NJ_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HHY6485054.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACV5NJ_000941"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACV5NJ_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HHY6485055.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACV5NJ_000942"
     CDS             19091..20464
                     /locus_tag="ACV5NJ_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HHY6485056.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACV5NJ_000943"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACV5NJ_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HHY6485057.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACV5NJ_000944"
     CDS             21519..21959
                     /locus_tag="ACV5NJ_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HHY6485058.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7474 a         3814 c         4810 g         5943 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt t
//
LOCUS       DBKOIJ010000032        21805 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000032 DBKOIJ010000000
VERSION     DBKOIJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.032"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1109
                     /locus_tag="ACV5NJ_000945"
     CDS             <1..1109
                     /locus_tag="ACV5NJ_000945"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HHY6485059.1"
                     /translation="FLTILLSLILTPVFKIISVQTGMVDKPNERRINKVPMPSAGGLP
                     IFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKSIGILIASL
                     IIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAAGISLI
                     GLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALFLGFMM
                     AVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLHHRLIS
                     LGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAILLFELIGLINEK
                     HQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1102..1902
                     /locus_tag="ACV5NJ_000946"
     CDS             1102..1902
                     /locus_tag="ACV5NJ_000946"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHY6485060.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1902..2729
                     /locus_tag="ACV5NJ_000947"
     CDS             1902..2729
                     /locus_tag="ACV5NJ_000947"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHY6485061.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2734..3441
                     /locus_tag="ACV5NJ_000948"
     CDS             2734..3441
                     /locus_tag="ACV5NJ_000948"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHY6485062.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3541..4407
                     /gene="rfbA"
                     /locus_tag="ACV5NJ_000949"
     CDS             3541..4407
                     /gene="rfbA"
                     /locus_tag="ACV5NJ_000949"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HHY6485063.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4421..4993
                     /gene="rfbC"
                     /locus_tag="ACV5NJ_000950"
     CDS             4421..4993
                     /gene="rfbC"
                     /locus_tag="ACV5NJ_000950"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HHY6485064.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5015..6043
                     /gene="rfbB"
                     /locus_tag="ACV5NJ_000951"
     CDS             5015..6043
                     /gene="rfbB"
                     /locus_tag="ACV5NJ_000951"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HHY6485065.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6141..6992
                     /gene="rfbD"
                     /locus_tag="ACV5NJ_000952"
     CDS             6141..6992
                     /gene="rfbD"
                     /locus_tag="ACV5NJ_000952"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HHY6485066.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7026..9059
                     /locus_tag="ACV5NJ_000953"
     CDS             7026..9059
                     /locus_tag="ACV5NJ_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7657 domain-containing protein"
                     /protein_id="HHY6485067.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9103..10383
                     /locus_tag="ACV5NJ_000954"
     CDS             9103..10383
                     /locus_tag="ACV5NJ_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HHY6485068.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10593..11399
                     /locus_tag="ACV5NJ_000955"
     CDS             10593..11399
                     /locus_tag="ACV5NJ_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485069.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11411..12631
                     /locus_tag="ACV5NJ_000956"
     CDS             11411..12631
                     /locus_tag="ACV5NJ_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485070.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12621..14207
                     /locus_tag="ACV5NJ_000957"
     CDS             12621..14207
                     /locus_tag="ACV5NJ_000957"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHY6485071.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14246..16384
                     /locus_tag="ACV5NJ_000958"
     CDS             14246..16384
                     /locus_tag="ACV5NJ_000958"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHY6485072.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(16752..17735)
                     /locus_tag="ACV5NJ_000959"
     CDS             complement(16752..17735)
                     /locus_tag="ACV5NJ_000959"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HHY6485073.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18113..19504
                     /locus_tag="ACV5NJ_000960"
     CDS             18113..19504
                     /locus_tag="ACV5NJ_000960"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HHY6485074.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19520..20560
                     /locus_tag="ACV5NJ_000961"
     CDS             19520..20560
                     /locus_tag="ACV5NJ_000961"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHY6485075.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20579..21664
                     /locus_tag="ACV5NJ_000962"
     CDS             20579..21664
                     /locus_tag="ACV5NJ_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HHY6485076.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            21697..>21805
                     /locus_tag="ACV5NJ_000963"
     CDS             21697..>21805
                     /locus_tag="ACV5NJ_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HHY6485077.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKK"
BASE COUNT         7063 a         3557 c         4467 g         6718 t
ORIGIN      
        1 tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc atttcagtac
       61 aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca atgccttcag
      121 cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta ttccgtaaaa
      181 ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc atagtcacag
      241 gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg atcttgattg
      301 cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg cttcctttta
      361 ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg atcttcggta
      421 tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg atttcattga
      481 tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct acagtgtata
      541 ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct tacaattttt
      601 atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt atgatggccg
      661 tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct ttgctgatcg
      721 tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg
      781 tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt ttaggcttta
      841 ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct ttcacagcta
      901 tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc atgttgtttg
      961 ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag tttattttaa
     1021 aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag aaatatttga
     1081 agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct tattagtatt
     1141 gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc aactgtaaaa
     1201 gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga gtacaaaaaa
     1261 agactaacag agtataggaa atatccctac atcgatatct attttcatga tgaaaattcc
     1321 ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta tggcattatt
     1381 tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt gttattgaaa
     1441 gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg aacaactcag
     1501 cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt gcctttcaaa
     1561 tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg attgcctact
     1621 gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga tattgataaa
     1681 tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga caacgacttg
     1741 tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta tgcagttatc
     1801 catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct
     1861 atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca gaaatattag
     1921 tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga ccattaagca
     1981 aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct caaaggcagc
     2041 aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa aatcctggtt
     2101 tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt tgtctcgtca
     2161 ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt aaagtaacct
     2221 ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga caagtttctc
     2281 ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt gaaggcccaa
     2341 cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa
     2401 agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg ctgttttgga
     2461 aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa catgatcttt
     2521 cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta caagcagaaa
     2581 atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga caattattgt
     2641 taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg atgactttgc
     2701 gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt
     2761 aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct ttcagcagaa
     2821 gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat attaactgaa
     2881 tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg attgatcgct
     2941 aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc agaccaagat
     3001 gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca gaatccggat
     3061 cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga gatccatgat
     3121 tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat aagaagtcac
     3181 tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt gccgatcccg
     3241 tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa gactgggttc
     3301 ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga gattgaaaca
     3361 cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc gttgatcaaa
     3421 cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc atgacaaata
     3481 taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc
     3541 atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt aacaaaggca
     3601 acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc aatgtctact
     3661 ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga tacaccacgt
     3721 ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata tgcagtacaa
     3781 gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat tggagatgat
     3841 agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc taaaatgttg
     3901 caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt caatgatcca
     3961 gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat tgaagaaaag
     4021 cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga caatgaggta
     4081 atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat tacggatgtc
     4141 aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg cggatttgct
     4201 tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat cgaaacaatt
     4261 gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg tatgggctac
     4321 atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca atacggtcaa
     4381 tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa cagatacaaa
     4441 attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg gtttcttcac
     4501 agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact ttgttcaaga
     4561 caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc aaaaaggaga
     4621 agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg tagtagtgga
     4681 tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat ctgaacataa
     4741 tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt tgacaccaaa
     4801 tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg ggggcatcgc
     4861 atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag cgattatgtc
     4921 ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc catttgttta
     4981 cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg ttaccggagg
     5041 tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc cagaagtgca
     5101 tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg ctggacttcc
     5161 tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag tggaccgtct
     5221 agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg ataattcatt
     5281 aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac ttttagaagc
     5341 ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt acggcgatct
     5401 tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga
     5461 aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag atctgttagt
     5521 aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt caaacaacta
     5581 tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata tcttaagcgg
     5641 tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc atacgaatga
     5701 tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa cctatctgat
     5761 cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag aaatgatggg
     5821 tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact tgcgttatgc
     5881 aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta cgaacttccg
     5941 tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt ggaaaaaaga
     6001 aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag aaaatgtcac
     6061 tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa
     6121 atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa ctaggtacag
     6181 aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat gcaaaagact
     6241 tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa cctgatgtta
     6301 ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca aaagaattag
     6361 atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa gctgtgaatg
     6421 ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa aatgtagaat
     6481 acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa ttattaggcg
     6541 aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct tgggttttcg
     6601 ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac
     6661 tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg gcagaattta
     6721 tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg aacgaaaata
     6781 gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta gaagttgcac
     6841 ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc
     6901 tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca cttgcgcaaa
     6961 tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag aatttggagc
     7021 atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa gcgaaccagg
     7081 cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt ggcgttttag
     7141 tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt tgggataaat
     7201 atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag aatcgggaag
     7261 tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct gaagaggact
     7321 ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt gcgtataatt
     7381 ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc tttttcctcg
     7441 gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg
     7501 gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta atcggtgcat
     7561 ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg ggcgatttga
     7621 ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat catgataaaa
     7681 aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg tttattttag
     7741 tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat tttgttggcc
     7801 tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg ttgattggag
     7861 gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca aaagacgcat
     7921 tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga ggaaagtgga
     7981 cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa gatatcaact
     8041 attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta ttcctagcaa
     8101 gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga cgtattttga
     8161 tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa tggctagcag
     8221 aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt agttttgcgg
     8281 cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt attcctattt
     8341 ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg attacgcatt
     8401 cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg ttgggaacag
     8461 tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct tttgccgcct
     8521 tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg
     8581 aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt aaagtttggt
     8641 tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc ttcaatacag
     8701 tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa gaatacgaga
     8761 agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat acaaagttca
     8821 ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca gaaaaattag
     8881 ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta tatgaagcgc
     8941 agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa gtagaatatc
     9001 cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat
     9061 aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat aattcaagca
     9121 atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt actgacgcta
     9181 gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat atataacaat
     9241 agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt ttataaagca
     9301 aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt cttttcttac
     9361 tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt gattatttta
     9421 ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat ttatctgatt
     9481 gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat ccaagtacca
     9541 gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat caatacgaat
     9601 ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc cagttctttt
     9661 gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt tctcgggatt
     9721 tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat attggatctt
     9781 ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac ggttacgatt
     9841 cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc
     9901 ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc tgaacaaatt
     9961 ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg aaaccacaat
    10021 agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc tgctttgctg
    10081 cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg gcagatgact
    10141 tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac attgacttca
    10201 tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg atatgatgaa
    10261 ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat gacttatgat
    10321 gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca acgctatcat
    10381 tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt tggtttattg
    10441 caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta tcatagaaaa
    10501 ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg cacttgactg
    10561 taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat ttatttatga
    10621 aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac ttcaaatcaa
    10681 gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta tttactgtat
    10741 tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc
    10801 catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca gatgcatttg
    10861 catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc gtattcaata
    10921 ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta ttcttcattc
    10981 tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg tctttacagc
    11041 tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg ctgacggcat
    11101 cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg cagttgatca
    11161 tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt gtaagcatat
    11221 tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt ttaagtgaag
    11281 cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg ttagtaatgt
    11341 tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat gtgctgtaag
    11401 gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat cttataatat
    11461 gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga aatcctatca
    11521 tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg
    11581 tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta cgggagtact
    11641 gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc tagaactagg
    11701 atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg gaatggttct
    11761 aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat ttgctgacat
    11821 cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg ttcgtctagc
    11881 ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag cattggcagt
    11941 aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca agaactcggg
    12001 aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt gtgatcgttc
    12061 ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag taacgacgaa
    12121 ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata aaaaccaagt
    12181 agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt tggataaaga
    12241 caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag taacttatga
    12301 agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa cagtcgacaa
    12361 tttttatgta tgcggactta atactctact agatggtatc aagattcctt ggaaaaaagg
    12421 agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg aatattattt
    12481 tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga caaagtacat
    12541 gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag accataaatg
    12601 ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat gaacaaagca
    12661 gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag tttggtcccg
    12721 gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg agcattgttg
    12781 agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga ttttatggtg
    12841 atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat tacgtcttgt
    12901 ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa gtcagagaat
    12961 ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat aattctgtca
    13021 tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta gatgaaaccc
    13081 ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg ggattattca
    13141 ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa tcaacttatg
    13201 aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt gaagtctacc
    13261 aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca gagccacaaa
    13321 attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat gaaacaatcc
    13381 aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc cccgtatcag
    13441 aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc gaattcagcg
    13501 cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc aaaacagtaa
    13561 atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg gatatttctg
    13621 ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca ccaacaatga
    13681 aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt tctgagaaaa
    13741 atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga ctagaccata
    13801 cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa tggacaataa
    13861 aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa aaagaacttt
    13921 ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa atcatcggat
    13981 ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct gaccaatcgc
    14041 catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa cagctcgcaa
    14101 aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt tcattcttag
    14161 tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata atgattaact
    14221 tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca
    14281 aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt
    14341 gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg aagcaacaca
    14401 tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc aaagcggttt
    14461 taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc aaacagcgag
    14521 acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg acggcattga
    14581 atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg caaggactca
    14641 tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat cgatcgacta
    14701 tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga aagaagcaca
    14761 aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg ttgaaatcaa
    14821 atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt gggaactatg
    14881 tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat cctatcaagc
    14941 aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca gtctggcaac
    15001 taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata atgatgatga
    15061 attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc cagaactgga
    15121 tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg atccgcattt
    15181 caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc acttaggcgt
    15241 atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg aaggttctca
    15301 agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata tttaccacat
    15361 tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta atggcgaagc
    15421 caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt ctagaagaaa
    15481 catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg cttatgacgt
    15541 cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg aagatctaaa
    15601 aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa tcattattgc
    15661 ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca agcatcagct
    15721 gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc gaattaacaa
    15781 tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg atacagaagt
    15841 gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc gaatcggctg
    15901 tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag tattattagg
    15961 aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt gtggttattt
    16021 cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta tgatggtcaa
    16081 ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag cattcaacga
    16141 tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg cacatcaagc
    16201 agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag aaaaacaaag
    16261 acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata ttgcgcatga
    16321 tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt tgagaccaga
    16381 ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg ctttttatag
    16441 ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag gttgggacag
    16501 aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg
    16561 aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg tttttaaaga
    16621 atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca gagcgccaat
    16681 agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc gatattttat
    16741 tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa agccactcta
    16801 tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt cttagcgtca
    16861 atataaagaa catttccact attaccaatt gctgaatata tttcgttctc agaatctaat
    16921 acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact ggttctaggt
    16981 ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata catcccttta
    17041 tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa accatatcct
    17101 gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga cttatccatt
    17161 gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt tggtatccct
    17221 ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact tgtctgatac
    17281 aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc ggttatacct
    17341 tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga acagactttt
    17401 gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg cttgattgta
    17461 atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata atctaatact
    17521 ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag caaagataca
    17581 atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc attccattca
    17641 tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt tggttgtcga
    17701 aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac tttcttctat
    17761 aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta atacaggtat
    17821 tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata aaggttcact
    17881 ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc aacattagtg
    17941 aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag aggtgtataa
    18001 agctttttat tagacatttt taaattccat tacaatttgc ttataaacta ggtttttaaa
    18061 ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat atatgaataa
    18121 aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg ttattgtata
    18181 caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc cattacgaaa
    18241 tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg cgttgaacat
    18301 tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt ttgtcacagt
    18361 catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg gccgctggtt
    18421 tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt tcttaagtat
    18481 ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa aggtcgtcat
    18541 tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa agaaaaacaa
    18601 caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca aaaatatgat
    18661 taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa aaatcaaaat
    18721 ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat ttgaaaactt
    18781 gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg aagcatctgg
    18841 atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca tcgtttcact
    18901 tgttttatta gttattgctt caccatttat gttattcaca gcaattttag tcaaagtgac
    18961 atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa aggaattttc
    19021 aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac cagttttagc
    19081 taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg tccgctttga
    19141 tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag gaccacgtcc
    19201 tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact acttacggca
    19261 taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg tctctgatta
    19321 caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga tgcttgattt
    19381 aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc aaggacttga
    19441 agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa aaatctttcg
    19501 ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac cagtatacaa
    19561 tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt atgaagagta
    19621 cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca ttcaagaact
    19681 gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc ccggattagc
    19741 aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg atgcagatga
    19801 cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga gtccaaaagc
    19861 ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa tcgtttccga
    19921 aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta taaaagacat
    19981 gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct atcgtagcga
    20041 tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg atcgaatatt
    20101 taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag tagaatatat
    20161 ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca agttcgaaac
    20221 atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata aagacggtat
    20281 ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata gtacctctgc
    20341 aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc cagtgatccg
    20401 agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa agttgcttta
    20461 caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg ttgaagtgaa
    20521 aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg aaaatataat
    20581 gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt ctttcatgat
    20641 gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga tttatgaaga
    20701 ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc gtatttttgt
    20761 tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata agttactaaa
    20821 aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa ttaatttatg
    20881 gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa ctgaagatgg
    20941 aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat tgttgttaca
    21001 aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt ttcaaaacca
    21061 cccctttgag atagttcata atggtattga tttagagctt tatagtaaaa attcagaagc
    21121 taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg tgggaaacat
    21181 tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt tagaaatact
    21241 aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta gaaataaact
    21301 gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag gagaaagaaa
    21361 cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc tttatgaagg
    21421 tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct tagcagatac
    21481 tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag atgaaacacc
    21541 acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa cacaagaact
    21601 aaagagttat gatgtttcgt acactactga acaaatggag agtatatata gtagagatca
    21661 ttaaaaatgt tcaagatatt ttaggagtga ttatttttga aaatatgtta tttgatattg
    21721 gcatacaatg atattggcaa cttaaagaga ttggtcaatc gattgaacga aaatgcagtt
    21781 tttattattc atatagataa aaaaa
//
LOCUS       DBKOIJ010000033        21789 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000033 DBKOIJ010000000
VERSION     DBKOIJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.033"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..964
                     /locus_tag="ACV5NJ_000964"
     CDS             <1..964
                     /locus_tag="ACV5NJ_000964"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HHY6485078.1"
                     /translation="KFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYKG
                     HDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVAY
                     IPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGIG
                     LDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSNA
                     FLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSKY
                     FHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1063..2235)
                     /locus_tag="ACV5NJ_000965"
     CDS             complement(1063..2235)
                     /locus_tag="ACV5NJ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485079.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2222..3367)
                     /locus_tag="ACV5NJ_000966"
     CDS             complement(2222..3367)
                     /locus_tag="ACV5NJ_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485080.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3381..4305)
                     /locus_tag="ACV5NJ_000967"
                     /pseudo
     CDS             complement(3381..4305)
                     /locus_tag="ACV5NJ_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4392..5501
                     /locus_tag="ACV5NJ_000968"
     CDS             4392..5501
                     /locus_tag="ACV5NJ_000968"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHY6485081.1"
                     /translation="MTFVTMKESAMHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYL
                     LIAVLFTGVSYLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFL
                     LGFLLISTVGNHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQEL
                     SEQNKALALSQETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLK
                     EPLENLSATVHTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNE
                     TQQNCFLMVIKEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGL
                     KTMRQRVQEQKGQVHIFQENQQFQLTIILPREGHQ"
     gene            5498..6126
                     /locus_tag="ACV5NJ_000969"
                     /pseudo
     CDS             5498..6126
                     /locus_tag="ACV5NJ_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6236..6463)
                     /locus_tag="ACV5NJ_000970"
     CDS             complement(6236..6463)
                     /locus_tag="ACV5NJ_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HHY6485082.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6566..9154)
                     /gene="recD2"
                     /locus_tag="ACV5NJ_000971"
     CDS             complement(6566..9154)
                     /gene="recD2"
                     /locus_tag="ACV5NJ_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HHY6485083.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9174..9833)
                     /locus_tag="ACV5NJ_000972"
     CDS             complement(9174..9833)
                     /locus_tag="ACV5NJ_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HHY6485084.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9882..10385)
                     /gene="trmL"
                     /locus_tag="ACV5NJ_000973"
     CDS             complement(9882..10385)
                     /gene="trmL"
                     /locus_tag="ACV5NJ_000973"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HHY6485085.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10606..11472)
                     /locus_tag="ACV5NJ_000974"
     CDS             complement(10606..11472)
                     /locus_tag="ACV5NJ_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HHY6485086.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11581..12222
                     /locus_tag="ACV5NJ_000975"
     CDS             11581..12222
                     /locus_tag="ACV5NJ_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HHY6485087.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12284..13645)
                     /gene="mgtE"
                     /locus_tag="ACV5NJ_000976"
     CDS             complement(12284..13645)
                     /gene="mgtE"
                     /locus_tag="ACV5NJ_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HHY6485088.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13663..14562)
                     /locus_tag="ACV5NJ_000977"
     CDS             complement(13663..14562)
                     /locus_tag="ACV5NJ_000977"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HHY6485089.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14564..15361)
                     /locus_tag="ACV5NJ_000978"
     CDS             complement(14564..15361)
                     /locus_tag="ACV5NJ_000978"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HHY6485090.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15339..16043)
                     /locus_tag="ACV5NJ_000979"
     CDS             complement(15339..16043)
                     /locus_tag="ACV5NJ_000979"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HHY6485091.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16174..16750
                     /locus_tag="ACV5NJ_000980"
                     /pseudo
     CDS             16174..16750
                     /locus_tag="ACV5NJ_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            16868..17545
                     /locus_tag="ACV5NJ_000981"
     CDS             16868..17545
                     /locus_tag="ACV5NJ_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HHY6485092.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(17624..19432)
                     /gene="pepF"
                     /locus_tag="ACV5NJ_000982"
     CDS             complement(17624..19432)
                     /gene="pepF"
                     /locus_tag="ACV5NJ_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HHY6485093.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(19608..20606)
                     /locus_tag="ACV5NJ_000983"
     CDS             complement(19608..20606)
                     /locus_tag="ACV5NJ_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HHY6485094.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(20832..21485)
                     /locus_tag="ACV5NJ_000984"
     CDS             complement(20832..21485)
                     /locus_tag="ACV5NJ_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HHY6485095.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         6426 a         4672 c         3784 g         6907 t
ORIGIN      
        1 gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg gctataaagt
       61 agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac ttcattatag
      121 tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa cattgattcc
      181 ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca tcgtaggagg
      241 tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag aattcccagt
      301 tgcttatatt ccagcaggtt ctggagatga ttttgctcga ggggcaggcc ttccaaaaaa
      361 tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag tccacataat
      421 ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt tcggtatagg
      481 attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc gattgaacaa
      541 atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat tcactcaaaa
      601 aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa acgcattttt
      661 atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc caactgctga
      721 tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct ttaaaatctt
      781 gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt tccatcacta
      841 cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag aagatggaga
      901 agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt tgttttggtg
      961 ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg gaaaaaagga
     1021 ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct ttgcccaatt
     1081 gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg aaaacaccgc
     1141 agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg tttgattgta
     1201 actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg gtgtgaatgc
     1261 cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc caccgataaa
     1321 gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt tttttaccaa
     1381 tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag tatttaaaat
     1441 atgataccat accgttatat cccacgaggt gtggatcact aacagaacca ttagaagtaa
     1501 gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt tgcgattcaa
     1561 gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc gattaaatgc
     1621 tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag acaagaaatt
     1681 aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac ttagctcaac
     1741 ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga ttgttttatc
     1801 ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg taacaactgt
     1861 tttagaaaag gcatctgggc gactctgtat cttgatcgtc ggcatctttc ctacttcgac
     1921 ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa aataaagagc
     1981 atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct tgtcattcaa
     2041 atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg cgattttcgc
     2101 atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg tggcaacaac
     2161 agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg tccaaatcgt
     2221 tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac gcaaaaagga
     2281 aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca gtgtagaaat
     2341 aaagtttata caaactttcg ctgatcaagt tgacgagatt taattgacca acaatcggaa
     2401 tatgaagatc aatccaatac ttcacttgtt ccgaccccat cataccagca aaaaagctca
     2461 tcgcaatcgt cactgaaatc aagatgctat ccttttgcac cagattcatt tttggtatca
     2521 aattaccaac caatgtacct aacaaaatcg cacacaacga ccccagagcg gtaacaatca
     2581 tcaaccattg ccaacgttca ccaaagtcta cttgatagat aaaacgaaag acagctaaca
     2641 taatataaat ctctgtcaaa acaatcgtaa agcttgctgc caaattagcg attgaaacta
     2701 acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga tttgcttgct
     2761 gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt ccaatcagag
     2821 tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct tgaaaagata
     2881 cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact tctgccactt
     2941 tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc atcaacgtct
     3001 gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg agataaaaac
     3061 cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt tcctgtactt
     3121 caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa ttttccgaaa
     3181 gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcgaga ttgccacctt
     3241 gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga aaggccaatg
     3301 tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta taaaaatgcc
     3361 aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc taaaaagaca
     3421 tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc ttcttgtaag
     3481 attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg gagctgacct
     3541 tttttataag cgattccatc agaagctatt gttcgctgac aacacgtttt ctgggagtaa
     3601 tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata tcttttagag attctttggt
     3661 tcctttagca atcacctgtc cttgatccat gatcacgatc tggtcgcaaa gaatttctac
     3721 ttcttccatg taatgtgttg tatagacaat ggttgctcct tgtcgattca gctccttgat
     3781 actttctaaa attttattcc gactttgggg gtctacagct actgtcggct catctaaaaa
     3841 aattaaccgc ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt tcaagccacc
     3901 actaagttct cctggacgaa acgaaacata tttttctaat cccactaatt gaatcacctc
     3961 ttcaatcaag gctttccttt tctctttttc ggaaacatat aaaccgcaaa aataatcgat
     4021 attttcttgc acacttagtt cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat
     4081 tgccttgaca gcataatttt caggagtcat tgttgtcccg aacaatttaa tggttccttt
     4141 gtcataattt aacaaagaaa gcatacagtt gatggcagtc gatttcccac tgccgtttgg
     4201 tcctaacaga cctaagactt ctccttcttg tacttgcaga tcaaaatgat tcaatgctac
     4261 aaaatctcca tagcgtttga ctaatttatc aatttcgata atcatcatga aaaactcctt
     4321 ccaaatttct tgttgatctg actataataa agtgcaagaa aagaaactag tgacgattgg
     4381 ctaaaccaca catgacattt gtcacaatga aggagtctgc tatgcataaa tctcggctac
     4441 tcaacgaaac tagctttcta ctggttatct cattgctttt gctggtaagt caaggattcg
     4501 ttgctatgca agttatctat ttattgatcg ccgttttatt cactggtgtc agttatttac
     4561 tgcctacaaa gccaaattgg attttccaag ttattctatt tcttttatgt gcctttttac
     4621 ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc
     4681 ttgattactt gtttttgtta ggttttttgt tgatttcgac ggttggtaat catcccccca
     4741 tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt ttacttacga aaaaaagatc
     4801 aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa agatactcat tgggaaaaac
     4861 aacaagagtt atcggaacaa aataaagcat tggccctgtc acaagaaaca caattagcct
     4921 tgcaaattgt ggaagaacgg aaccgaattg cccgagacat ccatgacaat gtcggtcatc
     4981 tgctgtcaag tgcgatttta caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac
     5041 taaaagaacc attagaaaat ttaagtgcca ccgttcacac tgggatggat cggattcgtc
     5101 aaagtgtcca tgacctccat gagacatcga tctcttttca acactcctta gagtttctga
     5161 tcgctgattt tccttcttct gttacgattg aaggaaactt gtttgaagga ttgaatgaaa
     5221 cgcagcaaaa ctgcttctta atggtcatca aagaagcctt aaccaacagt atcaaacata
     5281 gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc ggccttttat cgcttgcaaa
     5341 tcacgaatga tggcaccagc ccacaaaaaa attctgctcc cggtattggt ttgaaaacga
     5401 tgcgtcaacg tgttcaagaa caaaagggac aagtccatat tttccaagaa aaccagcaat
     5461 ttcagttaac gatcatttta ccaagggagg gacaccaatg ataacagtag tcattattga
     5521 gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa agagatccgt
     5581 gtgttaggca ttggtcattg tgccaaggat gcgctagtct tatacgaaac ccatcgtccc
     5641 gatgttttgc tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc tgccaaagaa
     5701 attttggcta aatttcccaa agccatcctt ttgcttttga cgacattcaa agatgaagaa
     5761 tacatccgcg aagcattttc catcggtgtc aaagggtatt taatcaaaca gaacttgcaa
     5821 gcgattattc cttctgtcaa atccgcttat aatggacagg ccgtatttgg taatgaaatc
     5881 atcgagactt tcactcaaat gatgaagaat aaaccgacga ttgatagtca tgcttatgct
     5941 tcttttagtg aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa aaacaataaa
     6001 gaaattgcag atgccttgta cttgagtgac ggcactgttc gcaattacat cagccaatta
     6061 ctagaaaagc tagatttacg ggatcgcacg caattggcta tctattttta tcagcatttg
     6121 caataagtct tatctaacac aaaaaaacct gaatgatgct ccttcgcatc attcaggttt
     6181 ttttcacatc ttctattcaa ttgtcccgca aaccatttcc tcacgattga aagaatcaag
     6241 gattcgcagg gctgccagga ttcgttggat tgttcggcgt gttaggcgga aaaataggcg
     6301 ccttgtctaa cgttttttct agttttgatt cttcacgatc gtctcgttct acatcaggac
     6361 tttcattcaa tttgaattct ttttccatct catctaactt tttaaagtca ttgccagatt
     6421 cttttgctaa tttcgctaat ttatcttcgg tttctttagc cataaagtaa cgcccccctt
     6481 ttgatttcag cttagcatat cacactgaaa aaacaaaaga aaacgctaac caacgatcat
     6541 ttttagctat ttctcatctt taaaatcaag cattttttgc cggtataaaa tcgtagggag
     6601 aactgttttc cataccaatc atcggatcta ctaactggtt atcgatcact tctgctgtaa
     6661 gaacgaaatc tttagaaaca gcaggttcta cctcttcttt cttttcttct ccaacaaatg
     6721 aagaaaacag atctggttcc actgtttcca ttacttggtt tttcgtttca ttcgtcccac
     6781 tgtcatcttc tacaggtagc ttttctttta caacccgtgg ctcttttgta tagttcccat
     6841 cttcaaatgg atcatttccg accaaagaat cttcataagc tgctagtttg gtccggagag
     6901 tgatattcat tttatcatta tccatgatcc ttgatttcaa cgtagtcatc cggcttgctg
     6961 attcattcgc tacacaagtg acataggctt gctcttcccc taacaaaatc aacagttcct
     7021 tactgcgagt cactgccgtg tagagcaaat tacgctgaag catccgctga tattgatgga
     7081 ccatgggcaa aataaccatc ttgaattcac tgccttgtga tttatggatt gagcaacaat
     7141 aagaaagagt gattttattc cattcgtttc ttttataagt gacctcattt gcatcaaact
     7201 ggataactaa ttcatccact ttatcttcag aatcttttgc caagatgata cccgtgattt
     7261 ctcccatatc tccattgaat acattcaatt cgggagtatt gactaattgg aggactttgt
     7321 ctcctatccg ataaacggtg tcgttccaat gtacttcctt ttttccaggt ttccctggat
     7381 tgaatatctc ctgcatcatt ttatttaacg catcgatccc tgcaggtccc cgatacattg
     7441 gtgctaatac ttggatatct tgtgcacgga atcccttatt tttcgctttt tccacaattt
     7501 tagagatcaa gggttcaatt tgataagcat cacttcggaa ataggaacga tctttttgat
     7561 tctttgtgaa atcttttggc agacgtcctt ctttgatctc atgggcaaga ggaataatac
     7621 tagaaccatc accttgccga taaatctgat tgagttccat tttgggaatt tgttcgattt
     7681 ccaaaagatc atggagtact tgaccaggac cgacagaggg aagctgatct ttgtctccta
     7741 ctaagataac ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc agccatgtat
     7801 cgaccataga catctcatca acaatcaaaa gtccgccttc taattctttc gtactgactg
     7861 aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg gatcgtactg gcaggcagac
     7921 ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg tgctgcaagt agaataggaa
     7981 aaatttcttg ggtatatttt gaaggatcca attccagtcc gttcaactct gcaaaaagat
     8041 tgacgattcc gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt aaaataaata
     8101 gaggggaacg gattgcttca gcgatagctt gttcttgcga aggtccatac tgtatgccga
     8161 atattttttc gatacgccga atattcttct gcacttcttt ttcaggataa tggatttctt
     8221 ttttacgact gagaaggcgc tggatcgaat taccgatccc ccattcagaa aagtacaaac
     8281 tgttttcgta aagattagtc tcttcttgtt gtatctttcc ttcttcaaca agttggataa
     8341 ttacagtagc aacctgatca ggctggattt caattgggcg acttgtttct aaaatacgga
     8401 tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc tcctgtttga actgcatgct
     8461 gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg tgaatctgct gcaataccga
     8521 tttgttcggc tagattatct gcacgtttga acccgattcc ttctatgtct tcgactagct
     8581 ggtacggatt ttcttcgatg atatccaaag actcgttctt ataggcctgg tagatactga
     8641 aagctaactg actaccgaac ccgtaacgat tcagtcccat gatcacttga tccatcccgt
     8701 ggtttagccg aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa ccggagattt
     8761 gttttaatac cgaaggatca gcaatgattt tttcgatagc atcttctccc agcaattcta
     8821 caattttttc tgctgttttc ttcccgattc ctgggaattt ttcgctggaa agatacgcta
     8881 cgatcccatt tgcagttgtt ggcttttctt gctcatatct ttcgagttta agctgccttc
     8941 cgtatttagg atgatccact aattctccat aaaaacgata agtctcattt tcttgaatat
     9001 cgccgaaact tccggtgact acaatctctt tttcacggta atcagctgtg gtttcactga
     9061 tgtgtaccaa aagcactttg taaaaattac taggattttg aaaaaaaatc gcggcaactg
     9121 taccaacgaa ataaggcatt tcttctggct gcatcggaag ccgcctcctt cttctatagt
     9181 aaaggttctg gtgctgtttc agttcctaaa aaatctgttt ggttccatac tttcagttca
     9241 tatggcaata atctgcaagg ctcttcagtc tctaaaatcg tcaaactact attgaacaat
     9301 ccacccattt cacgcaactc acttagctct tttcctgtca tccattgaat ggctgcagtt
     9361 aaagaagctc catgtcctac gaataaaagt gggccatctt tatgttgggc aaccgcatct
     9421 gctacaacag tttcgatacg tgtgattgcc gattcaatcg tttctccgtg aaaaggagaa
     9481 ggatcataca gatctaaatg attacgcaaa ttcggcagat tatccggata tttctggtac
     9541 atttcatcga tcgactgacc ttctaaagta cccagcccta gttctcttag cttatctgta
     9601 taaaaaatct ctaccggacg agcaagctct ttgttgatac cttctgctgt atcacgtgct
     9661 cgctttgctg tactggaata aattgcttta aaaggaacat attttacctt tttcccaaat
     9721 gctttgattt cttcgtaact ttgtggcaaa agcggagaat cgccattgct cccttgaaaa
     9781 cgtctttcct gattccattg tgtcttccca tgtcgggtaa aatatagttc catacgcagc
     9841 aaactcctgt ctttctttta ttttactcta taataaactg tctaatccag cttgtcattt
     9901 tcatagtgat gcgtcaattc taaattaggg aatccttgct ggcgaagtgc ttcatacact
     9961 atcattgcag ctgtattcga taaattcaat gagcggacat gctcatcatt catagggatg
    10021 cgtaaacact tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt tgtttctttt
    10081 ccaaataaaa agaagtgctc ttctccatca gcaaaatttt cgtcactata tactttatgg
    10141 gcaaattttg agatcaaatg tagtttacga ttacctaaaa atgttaaaaa agcttctaaa
    10201 ttactgtggt aagtaatatt tacatcattc caataatcca accctgcccg ttttagatgt
    10261 ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt cgtggctgca
    10321 cacgtacggg caatatttcc agtgtttgca gggattaatg gttcaaataa aacgatatga
    10381 ttcatcagga tcattcctct catatttatg ttcgtttcta ttttctgaca atttgatttt
    10441 tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc gagtcgttac gttagtgaag
    10501 ctcttctcac taacttttat cagaacagga acctgataaa aaccaatgaa aaaacaactt
    10561 gttgctaata taacaccctc aacttttttt tgcaacctct actcattaaa aactgctaga
    10621 aaaacgtttt ctcttagttc cttttaataa ttctatgtca attgtaatct tcttcaaagg
    10681 attcttatat aagtctgctt tgattcgctc ttctacctgc cattctaaag gtgacatggc
    10741 caaaagatct ttttgatttt cctctggtat ctcaaattca taggttacac gctgacgttc
    10801 agttaatttc attgcttcag caaacttggc tactacacgt tcattggaat aactctgttt
    10861 ttcaggttga ttcggataaa aagcctgacg tagttccttt aaataatttt cttgtggcac
    10921 caccttcaac acttctccat cttctgagag tacacgttgg aattcttggt aatgagaagg
    10981 cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat tggtcaaatc
    11041 agctacacac caaaatgctt cggcagattg ttctgtagct gcgtagactc cttcttttga
    11101 aatatcaaat ccaacagcat tttgaatctt tttcaatttt gaaagttcat tcaagaagct
    11161 accttctccg cagccaacat ctaatagcga gacttcttca ggtaaatgct gcgcaatagt
    11221 ctcaataacg ggctgataca tgccagattg gatcattcta cgccttggaa cgaacattcc
    11281 ttgatcgtat tctgttttaa ccgcatggga taaaaaataa agtgttcctt ttttggaaag
    11341 gtcaaaacgg tgattatttt tgcatatcaa gccattatca gactttttta cttcttcttg
    11401 gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa aactggcgtc ctcggtcaat
    11461 tttttttaac atagttcccc ctcatttctg ttttatctta ccgttagatg ctcactttgg
    11521 caactgctaa aaatatgata caatgcctag aagaaagtga aagataaagg agaagattag
    11581 atgatcaaag aattttgcgc tgaaaactac acatctattc ctctagcaat tgctagagga
    11641 gccgatcgga tcgaattgtg tgacaactta gcggtcggtg gcacaacccc gagtactggt
    11701 gtgatcgaag aaacgttaag ttatgctggg gaaaaagata tccccgtaat gacgattatt
    11761 cgtcctagag ggggcgactt cgtctataac gatatcgaat tgaaaatcat gcatactgat
    11821 ttgattgaag caaagaaatt agggacggat ggcgtagtat ttggttgtct tactccttca
    11881 ggctggctag acgaagaggc cttagaacta ttgatcgaaa cagcagaagg gttgcagatc
    11941 accttccata tggcttttga cagtattcct gaagatcggc aattcgaagc aatcgattgg
    12001 ctggtagaac atggtgttga ccgtatcttg actcatggcg gtcctgctga caaaactatt
    12061 gtagaaaatt tcccacggct taaagaattg attgcctata cagatggccg cttaatcatt
    12121 ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg tagctaaaga attgaacgta
    12181 agcgaagtac acggaaccaa aatcgtggat cttcagttat aatcttataa acaaaaaaac
    12241 atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt tttttacata aaatagccca
    12301 taaataaaga agcaatattg aaataaatca gtacactcgt caaatcgctc aaagtcgtga
    12361 taaagggacc gcttgctact gctggatcaa atcctagacg atccattagc atcgggatta
    12421 aactacctgc aagattcgcc actgtgattg caaatagcat agccatccct atgacaaacc
    12481 ccaagataaa attatgtttc caaaaaccta cgattagaaa aatcgtgatc cccgtaacag
    12541 ctcctgtgac aacgccggtc aagacttcag ccaaaacaag tcgaacaaat ccatgatctt
    12601 tctcatcaga aatcgcaagt cgacgaacag ccacagctag cgactgggtt ccagcattac
    12661 cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca cttaccaagt
    12721 cttcatagtg actgattaaa gaagcggtcg acatacctaa aaacaacaga gtgatcagcc
    12781 aaggcaaacg tcgagacgct gcctttactg gattttcatt aatttcttcc acattgaccc
    12841 cggcaagtcc tgaataatcg ctagcagctt catcatcaat aacgtcgatg atatcatcga
    12901 ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac aggaagagct aagaagtcat
    12961 aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact gaaagcactc
    13021 gttcgctcat cagatcagaa atcattgcgt cgtcatcatt cacgattagg tcgcgcagag
    13081 aaataacccc tactagctga ccttcttgat taacgacata gatgtaatag attgtttcag
    13141 ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg aacagtttga ttggctacga
    13201 tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa tggagaagtt
    13261 ccttgatttc actggcatcg tctgccgaca tcaaacttaa atatttagca atttgctttt
    13321 tatccagcgt attaagcaag tcaacagcat tatctgtata catctcagaa agcatatcag
    13381 ctgcatagct aggacgcatt tcatctaggt attctttcat atgttcgttg tcttcttcaa
    13441 tcacatcgaa catatccgct aattctttag gcgacagata ggaatagatc agctgcctgt
    13501 tctcttctgt aagagattgg tagaattgcc cctgctcata gatatgcatc tctaaaaaac
    13561 tgtttctgaa tgcctgaata ttattttccc ttaaatcttg agtcaactgg gcaaatcttt
    13621 cctctaattc ttgattttca tccaaaccaa tctcacctcg ccttattctt caaaccgtct
    13681 cacaactgcc tccatgtctg cggctaaagg ctgcttgaac tccagcaact cttctgtaaa
    13741 tggatggtaa aaacgcagat cataacaatg aagggcttgt ctttttattc ccagatccat
    13801 tcgcccgcca tacatatcat caccgagcag ggcacagcca attgcggaaa aatgaactct
    13861 tatttgatgt gtccttcctg tatgcagctg gatatcaact aacgcgattt ctgttagtcg
    13921 cttctctatc cagtactcag tctgtgccga acgtccttca tcagagacca ttcgcttcaa
    13981 tagcgaagaa taatctcggg cgatcggcaa atcgatatgt ccatgtgccg ataaatctga
    14041 aagccttcca ctgactaagg cctgatattt tttcacaaac ttcttttcac gaagttgcat
    14101 atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc atcaaaccag atgtatcccg
    14161 atctagtcgc gtcaccacat ggattacttg gttttcgtaa ccttgttttt tataataagc
    14221 ctttacacga ttagccattg tcccatttgg atggtattgg gcaggaatag acgaaacgcc
    14281 tgctggttta tttactacca gaacgtgttc atcttcatac acgatatcga tcggtgtttc
    14341 atccaaaagt acggtttcat gttctccttc agcaggaatc acaatggtta ccttatcatc
    14401 cttagctaga gaaaacaaga cattttctac ttgatcatta actttgattt gaccgccttg
    14461 aaattttatt ttcgctaaca gaccctttga gatccctttt tcttttaaaa aatattttac
    14521 ttgctgagaa tgatctttct cataatccca tgtaaactgc atatcaatcc tcactaataa
    14581 aggcatcttt cacacgatgc caaaaatgca tatgtcgata agaagcaaaa tggatccgtt
    14641 cttctgctat tcgataataa attgagcgga tatttgcttt tgcgacattc aattgatcaa
    14701 ccgttacaag ataatcttct gtattttcca attttatttc tagccattca tcatgggcaa
    14761 tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt cagtgaagca atctctgcca
    14821 actgaaaggc attgatacta ggatggatca cagcaccgcc gacactttta ttatatgctg
    14881 tcgaaccagt aggcgtagaa atcgtcaatc catctcctct aaaactttca aacagctcgt
    14941 ttttgatgta tacatcggct accatcgtac gattggcccg tttaatcgtt gattcattca
    15001 gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact gatccgaaca tccagcaagg
    15061 gataactaac actttgttcc cggttcgttc gtaagctgtc tactagttct tccagctcgt
    15121 aatcccgcca atccgtgtaa aaccctaaat gacctgtatg cacgcccaag aaacgcacat
    15181 cattcagaca atgactgtag tgatggaacg ccgacaataa tgtgccgtct ccgccgactg
    15241 agatgaccaa ttctggctca tgatcatctc gttgaatccc tgcttgttct aaaagtgcgt
    15301 tcaaacgttc tgtcacttct atagtctttg cttctttatt atgaacgatc gctactttca
    15361 tcccctgcct cctctctttc gctttctttc ttcaaaagcg ttcgatagta gacatcatct
    15421 gattttcctc ttccatggga gaataaccgc tgtgcttctt ggatttcttc acggatactg
    15481 gacatctctt catccaactg ataggcagct tctgccgcac gctgaagtcg ttcactcatt
    15541 tcttctggaa aaacgccttg atatttataa ttcagcgaat gctcaatcgt tgcccagaaa
    15601 ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt tcttctcacc gctgatcagc
    15661 tgtacaggat actcgatcac cagatgataa gaacggtagc cacttgcttt tttatttgtg
    15721 atgtaatctc gttcttggag aactttcatg tcttttcttt tacggatgat ctcaacaact
    15781 tgatggatat cttctacaaa ctgacacatg atccttaacc cggcaatatc agacatttct
    15841 tcttctaaac gtcccattgg aatatgccgt aattgtgatt ttgttacaat actatcgact
    15901 ggcttgactc tcccagtgac aaattcaatc gggacatgtc gattttgttc acggaactgt
    15961 ttgcggatcc cccgcagttt cactttcaat tctgatacag tttgttcata tggtgctaaa
    16021 aatgcttccc aatctcgttc catagctgat acctctactt cattaacatt cattgtttat
    16081 tctaccatat atgcaaagtt ttttcgtact cttgatttat gcattgacca aaaaaagaaa
    16141 attaggcaaa atagacaaaa aggagtcgat caaatgacac aaatgcttga aatcgaatat
    16201 aaatctatgc tgacaaagca agagtacgag atattcatcg ctcactatca attaacagat
    16261 aaagatttcc gtgtacagac taatatttat ttcgataccg ttgatgaaca gctgaagaaa
    16321 caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac attaaaaaca
    16381 cctgctgaaa aagggcgtct cgaaacaacg gataccttta taacagaaga agcagaagca
    16441 tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact ttctgatttg
    16501 aatatcgatc ctgcttcatt aatccaaacc ggaaagctta caacaaaacg ggctgaattc
    16561 cctattgaag aaggactttt agctattgat gaaagctggg gagacaatct acacgatttt
    16621 gaactggaac tagaggtaag gggatgcatc tgtgggaaaa attgctttta taaatttttt
    16681 aaaacgtttt tctttacctt atcgtcctgc taagaacaag atccaacgta tgcttgaagc
    16741 taaaaattag aaacctttca ttttcacaag gcacttgagt tcattttttt acttttttct
    16801 gttaagttat aggatgtgaa agaatctagt cactagttgt atagaatcta ttttggggga
    16861 gattcatatg atcgaaatct atttatttgt taatccttta ggggaatact gtttcgaaat
    16921 ggaacaacag ttattacaat ttatcgaaga agagtacggg acaccatcaa aagaaaagat
    16981 gcaatttcgc tttcttcctt tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa
    17041 aggaatttct cagaacaatc ttgaggaacg taatcactta ttctcaatga cttattctgc
    17101 agcattggac tgcaaagcgg cgcagtttca aggaaagaaa aaaggccgcc gttttttgat
    17161 caaattgcaa gaagcagtcg gctgtaatga cataccatat tctcaagaac ttgctgaatc
    17221 catatttgaa gaaatcggcg gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt
    17281 cgttaaagaa gtattctttt ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca
    17341 cccttctgca gtcgtttaca attatgcctg cgaccgtgat tttggtgttt tagtagaagt
    17401 aaaaggtaca agtagccttt ggagaatcac agagctgtgt aaaacaacag ataactcata
    17461 tcaagttttt aatggtgaac atttacatac aaatgattct cagttccatt caagaactag
    17521 ccatttacac ttgctttcta attaaaaaaa tacgtcgact cccttacaag agagccgacg
    17581 tatttttttc gtttctattc gagaaaatca aaaatagaaa gccttataaa cgatcgatca
    17641 attcttctaa ttcatttaga cgttgttcaa acatcgacat tgcatcttca atatatgcag
    17701 cctgtgtcat atcaactcct gcttttttca ttacttccac aggataatcg ctgttacctg
    17761 cttttaggta agccaagtag ttctctagtg cctctggttc ctggtttagt attttcttcg
    17821 ccagtgctga agcagcagaa aagccagtcg agtattggaa aacatagtaa ttataataaa
    17881 aatgcggtat ccgtgaccat tcgaatttaa tttctgggtc ttcttcgact gctggaccat
    17941 aatattttgc attcaattta ccataactat cacttaggta ttcactagtc aatggcacac
    18001 ctttttcatc ttccgtatgc atgaaatgct cgaattcagc gaattgtgtc tggcggaaaa
    18061 ctgttccttt aaacccatcc aagtaatggt tgagtacata agcccgtaca cgagggtctt
    18121 tttctgtttc caataaatac tccgttagga tattttcatt cgttgtcgaa gcaatttctg
    18181 ctaaaaagat ggagtagtcg ccgtacacat aaggctggtt cgaacgagtg aaataactat
    18241 gaacactatg tcccatttcg tggacaagcg taaatagctg atccagcgta tcatgccaat
    18301 tcaataaaat atatggattt gtgtcatagc ttcccgaaga ataagcaccg cttcgtttcc
    18361 ctttattttc gacaacatcg atccaacgtt cagagaatgc tttttctacg atggccatgt
    18421 attcttcacc cattggtttc agtgcttcta aagctttttc ttttgcttct tcgtacgtaa
    18481 aggtaattgg agcttcccct aagaccggtg tataaagatc atacatgtgc agtttttcca
    18541 cttctaataa acgtttccgt aattccatgt atcgatgtaa caaaggcaaa tgcttgttta
    18601 ccacgtctac caaagtatcg tatacacttt caggaatatg attattgctc aaagacgctt
    18661 ctctggcaga gctgtaatta cggactttcg ctttaaaatt atgtcctttt atatgtgtgc
    18721 ctaaagtaga agcaaatgta tttctaaatt gttcgtaaac actgtacaat cccttaaatg
    18781 ctgcttcgcg cacccttcga tccgtgcttt ctagcaactg accatacacg ccatgagata
    18841 attggactat ttcaccattt tctccttcaa tcgttggaaa aactagatct gcattattca
    18901 aaacagcaaa tgtatcactt gaagcatcaa agatttcacc tgctccagca agaagagatt
    18961 cctgctcagc agataagaca tgggctcgat tatctactat ttgctggata taatggcgat
    19021 agacttccaa ttttggttct tctttgaaat attgccaaat ctggtcatct gacaattgca
    19081 atatttctgg ttcaaaccag gaaacggctt cactaacttt cgaaaacaga ctgcttgctc
    19141 ttgcataaag cgcttggtaa tctgtatttc cagtgtcttg atcgttttta aggtgcgcat
    19201 atacataaat gacttcagtt tggcgataaa ccctcaatac gaattcaatc gcatttaaaa
    19261 attgagaagc gccttgatca agtgttcctt tgtgtttttc agattgtttt aactcttctg
    19321 ataattccaa atatttctca tcaaactctt ggtcgcttga aaagatcttg gtcaagtccc
    19381 aagttaaatt ttcaggcaat tcttctcgtt ttggcaactg ctttacttcc atgaaatcca
    19441 ctccttttct tcccttagct ctatctatca aaatagatca tagtctaaca tccacatgtc
    19501 acagcatcta acttttctac tcattcatgt gtctgcttcc atcaatttta gcataactaa
    19561 atcttgtttt acatagaaaa ccgattccta gcctattttt atacttacta ttttttcaaa
    19621 gaagacaagt gtatgcattc gttaaaaaac tgctgataga ccatctcttc atcaatcata
    19681 ggaaaaagcc attccctttt atgttctttt atttggcaaa gccatgagtt gaaatcagga
    19741 actttctggt tttccaaggc ttcttcgtac aacattcgat aaagaaaaac ctgttcttgt
    19801 aaataaaaga aaaaccgact tttttgatag atccacgatg atagatgcaa aagatgttta
    19861 tgacgcaagt agcattgttc ttgtatggat actgtattct tttgcttgtg aataagttgg
    19921 cgtgtcagcc atttcttccc atcttcactc actttttcta tttttttcat ttggttcatt
    19981 ttaatttcgt tactagtgaa tatttcttta aggtccctac tagaaaaagt ccagcagcta
    20041 ctgcaaaagg aaatatgccc tgatacagtc tctgtaatat gatgatacaa ataaagcttt
    20101 tgtttcttga gatcagcttt ccagcaatgc actcctcttt ttctattata gtagcagcaa
    20161 cttttttcta tcaatgagaa ttgcgattgt ccaagaaaag aattaccaag tatccaccat
    20221 acttggtaac tgttattcaa ataattttgg gttctttcaa taaatctctg gtgagacagt
    20281 ctagaacatt ggatttctat tgctagattc gccaacaaaa tatccggcct ttgccgcaac
    20341 ctcggcaaat aggcttccaa aaatactggt tctactgact gtcccagcca atccatgaag
    20401 cattccttta actgtaagtg ctcttttgat tctctttcac taaagaccga acagttatct
    20461 gatttttgat gggcaaaatg agcacatttt ttctttccct ttttcaaaat cactcttttt
    20521 cgacagccac gacaataata tttctttcct ttttctgcat gtttagcaaa acagcactta
    20581 ttatattcat tcattgcaag cagcattttt ctccctctga acttatttac gtctaagaat
    20641 ttatttacac gaaaaaagag ggcgtgaaaa agctattctg catcaagtaa taagaacagc
    20701 taaacaaaaa tagttcccaa tatttttcgt ttggttgggc ttgttaccgc agatgcaagc
    20761 ttttgaacac atttatacga aaaaaagact gcgacacaat ttttgtcaca gtctcttact
    20821 ataatgctga tttatacaaa ataaaatttc gttcgctcga tagcattacg cgccatcaga
    20881 cattgtccat attcattcaa tgtttctggt gtacgatcgc tcttttcagc aaactccaaa
    20941 attcgagcaa tctgattctc tacatccgct tggttgagat tttccatcca gaagtgaacg
    21001 actaggtaat agcgctcttc gaataaatac aaatcagtcc aagcagactc tagttcgaca
    21061 tctgttgcaa gttggatcac ttgttcaaaa tcttcaaaag caaaagttgc ttgtctttcc
    21121 tgttcttcta gggcgcttcc agataattct tcctccggat cgctgccaat aatctgctga
    21181 cgaagaaagt cactcacatc ttctgaatca acagtagaat cttctgattg agaaatttct
    21241 tcatccggaa gatttttact gataaacagt tctaatccgt ctccttttgg caaaacttgg
    21301 aaagtgacag cttcgcttcc tcggaactcc tcttcgatat caacttcttc caaaatacta
    21361 taaaagaaat tttcgatttc tttgtgattg ccaagtaaat ccaaaaatga aacgccccgt
    21421 tcctctaaat cttcatgacc aattagtaca cggatcgtat tttcattaat atgttccatt
    21481 tccattttgc tacacctcac tttgccacaa tgtttaatat agattcattg tagcgaaatc
    21541 agcttaaatg taaagaaaaa aaatctcgct gttaagcgaa tttaataaaa ccagcggatt
    21601 tttcaacctt tcaagctatc ttatttaact ataacatttt tggcagacga atactatata
    21661 gccgactttt cagctaattt tccctctcta tttttcgtgc aatcatcatc ttctactacc
    21721 tttttataca tctaatcaag aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc
    21781 tttgtaata
//
LOCUS       DBKOIJ010000034        21738 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000034 DBKOIJ010000000
VERSION     DBKOIJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.034"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(193..1092)
                     /locus_tag="ACV5NJ_000985"
     CDS             complement(193..1092)
                     /locus_tag="ACV5NJ_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HHY6485096.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1102..1458)
                     /locus_tag="ACV5NJ_000986"
     CDS             complement(1102..1458)
                     /locus_tag="ACV5NJ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HHY6485097.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1813..2109
                     /locus_tag="ACV5NJ_000987"
     CDS             1813..2109
                     /locus_tag="ACV5NJ_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HHY6485098.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2753..3706)
                     /locus_tag="ACV5NJ_000988"
     CDS             complement(2753..3706)
                     /locus_tag="ACV5NJ_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HHY6485099.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3729..4484)
                     /locus_tag="ACV5NJ_000989"
     CDS             complement(3729..4484)
                     /locus_tag="ACV5NJ_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485100.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4481..5440)
                     /locus_tag="ACV5NJ_000990"
     CDS             complement(4481..5440)
                     /locus_tag="ACV5NJ_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HHY6485101.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5433..6383)
                     /locus_tag="ACV5NJ_000991"
     CDS             complement(5433..6383)
                     /locus_tag="ACV5NJ_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485102.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6533..6985
                     /locus_tag="ACV5NJ_000992"
     CDS             6533..6985
                     /locus_tag="ACV5NJ_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HHY6485103.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7074..9341)
                     /gene="gshAB"
                     /locus_tag="ACV5NJ_000993"
     CDS             complement(7074..9341)
                     /gene="gshAB"
                     /locus_tag="ACV5NJ_000993"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HHY6485104.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9528..10526)
                     /gene="ftsY"
                     /locus_tag="ACV5NJ_000994"
     CDS             complement(9528..10526)
                     /gene="ftsY"
                     /locus_tag="ACV5NJ_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HHY6485105.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10537..11346)
                     /locus_tag="ACV5NJ_000995"
     CDS             complement(10537..11346)
                     /locus_tag="ACV5NJ_000995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHY6485106.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11355..14936)
                     /gene="smc"
                     /locus_tag="ACV5NJ_000996"
     CDS             complement(11355..14936)
                     /gene="smc"
                     /locus_tag="ACV5NJ_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HHY6485107.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14956..15642)
                     /gene="rnc"
                     /locus_tag="ACV5NJ_000997"
     CDS             complement(14956..15642)
                     /gene="rnc"
                     /locus_tag="ACV5NJ_000997"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HHY6485108.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15990..17771)
                     /locus_tag="ACV5NJ_000998"
     CDS             complement(15990..17771)
                     /locus_tag="ACV5NJ_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HHY6485109.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17797..18711)
                     /locus_tag="ACV5NJ_000999"
     CDS             complement(17797..18711)
                     /locus_tag="ACV5NJ_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485110.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18738..19700)
                     /gene="opp4B"
                     /locus_tag="ACV5NJ_001000"
     CDS             complement(18738..19700)
                     /gene="opp4B"
                     /locus_tag="ACV5NJ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HHY6485111.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19700..20644)
                     /locus_tag="ACV5NJ_001001"
     CDS             complement(19700..20644)
                     /locus_tag="ACV5NJ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485112.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20645..21649)
                     /locus_tag="ACV5NJ_001002"
     CDS             complement(20645..21649)
                     /locus_tag="ACV5NJ_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485113.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6076 a         4642 c         3849 g         7171 t
ORIGIN      
        1 tgtgattatc gttcaaaaaa agaatacacg cttgcatcta gtactctgcg agttacaaat
       61 catccgatca aataaacatt cgctgattat tttccgcaaa aaaagcagca tatccacgat
      121 aaatggatct actgctctta cttatcctta tgttttacaa ggtattttct tttacacatt
      181 actaatgctg tctcaatacg gaacgattgt catcgcttgt tcaaattcct cttgcaaatg
      241 ttgtgtttga taggcctgaa aagctgggtg atgggcacgc aagaggacac gtaatttcat
      301 aggatcatag ctgtctagca actgttcttt caagacaaaa tctcgggtca gctgccattc
      361 ataataataa ccaaaaggtc tgcagctgta aatcgtgtga ccgatggatt tatcttcaaa
      421 gcgacgatgc gtatggccaa aaactacttg tttgatattt gaataacggt caagtaattc
      481 tccgaatgat tctgatccta aaaatgcatt taatttgttc caacgctcat aaggagcaga
      541 ttgatacatg ataaatgccc gtttaggaac gaaatgagtg gataaaatca cctcaagttt
      601 ttttgcttcc agctggtcta gcaaattttt tgcagctgca agcatttctg cattgacctc
      661 gagatctgtt ttcccacgct ggatgaaccg atcataccaa tacaaacgct tcattcgtac
      721 gattgcctcg taatctttca attcggaaaa ctgatagtcg taccagccat tcatccctaa
      781 aagaacggtc tgctcattta aagcaatata ccgttcattt aaaaaatgtg ggtctggaaa
      841 atggtccatc atctgttcat tgggcacatc tgccaactcg tgattgccga aatgaaatgt
      901 cgtagataat cctttattga cgaaaaattc gacaacagct aggcaacggt ctactttatt
      961 agccgtatct ccggctagat gaagccgagt gatcttgtgc ttttgcaaaa cttgccaaag
     1021 ctgcatcagc tctgattcgc cgaatttatt gatgtccaca tgtaggtcgc tgatgatcgc
     1081 caattttccc attttatccc tctagtcttt tcgaacaact tgcaagttgc cttttctcaa
     1141 gccgcttggt ccttttaatt gtaaactatt ataagttggt tctaaagcaa gtgtggatgg
     1201 atcatccatg taaagtattg cactgtcttt gattttgatt ggtccaactg gtgtatcagc
     1261 cactacagaa taaagactta gatctgtttg tgtatctttt ttgacaatga tcaatcgaaa
     1321 tgccgtatcc aattggcaac tgacttggct ttctgcaaat ggaccgtcac cgttttcgat
     1381 atctaataaa agttgatcgt ctgcttctaa tttattcttc aatacttctt gtgcttcttt
     1441 tgtcacttct aatttcatct gtatcacctc atgtcataga ataatcactt tattcatcag
     1501 atgcaactaa tttgtctggt tctgactgtt ctcaatttat tggaaagtaa gtcattcgtt
     1561 tattatgatt tctcttatca caaaagagac aactttccct tttcttcgtt cagtttcttg
     1621 aacggaaaaa gacccaaaaa gcaacctgtt atttgtttaa ccttattcct ttcttaaata
     1681 acaaaaaata ctataaaaaa taaaaaacct ttaacaacta ttggaaaaag aattactttt
     1741 tgttcacgga ttattaacaa ctcttcggta gagtaaactt atcaaataaa agacttcttt
     1801 ggaggaatca ctatgaagga atatataaaa aacatctatt ttatcgaaga aacacaaaat
     1861 atcgagggtt cttatattga agtaaaaaca ctctttgtca atgaagataa aaccaaagca
     1921 ttggatattt ataaaaaatt agcttcaaaa aaaacgaata gttttggatt gatattaagc
     1981 gagtataaaa tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag
     2041 cttcccgccg atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca
     2101 catgcctaaa tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt
     2161 gaacattgtt tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg
     2221 acagaaaata gcttctcata ttttctagtc tagtgggctt attgccgaga agcagcagat
     2281 ggaacatcgt ttagaagaga ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg
     2341 gacagaaaat agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga
     2401 tggaacatcg tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat
     2461 ctaagtttcg ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa
     2521 tggatcacac tattttttgc cactccccat tctagaatca acccgtatga tgtagataat
     2581 gaattcacgt tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata
     2641 cgattttacg gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg
     2701 taagaaacat tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa
     2761 atgcttgatt cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat
     2821 accagacatc tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta
     2881 ccagttcatt ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt
     2941 ctactacaaa taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac
     3001 cgtgagtgga tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga
     3061 aaccgaatct agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta
     3121 gtgctttttt gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt
     3181 cctctaattg agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat
     3241 tttgctggaa agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa
     3301 tttgacttag atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct
     3361 gaagctgatt aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt
     3421 tataggatga aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa
     3481 caccaagagc atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat
     3541 tatttggcac ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt
     3601 gtgtgctgct tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta
     3661 aaccaaatga tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa
     3721 tgttagtttt agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc
     3781 atttcgtata attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta
     3841 aataactgtc catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata
     3901 tcatgaagaa caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc
     3961 atgatttgaa ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa
     4021 tccgtgtctt gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga
     4081 gtgtcaatcg attgatccgc tatttcttct aacccaatgt gttccagtga ttcctgaacg
     4141 atttcctcgt cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact
     4201 aattctttca cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt
     4261 ttggctaatt cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct
     4321 gtatcttttg gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt
     4381 ggaccgataa aagcagtcaa cttcttttca ggaatcgtta actgtacatc tttaaccact
     4441 gattttccat ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt
     4501 cgcgaatcaa ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg
     4561 ttgtattcca gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag
     4621 ctaccagaaa gcttccgata aatagctgac catgacgata agtagaaaag acacgataac
     4681 tgatatttgc gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc
     4741 ctgtcaaaag actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga
     4801 ggtttactgc ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata
     4861 acaggtacca gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat
     4921 tgccaaagct tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga
     4981 cttgtaaaaa ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta
     5041 ataaaaaaag attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc
     5101 cgaccatcag cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca
     5161 ctcctccgat aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca
     5221 atactgaagg agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc
     5281 tgacagctag cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa
     5341 aatgccagtt tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa
     5401 caagcatacc aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga
     5461 agataaagga agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa
     5521 ggaaaaatca acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa
     5581 gcagttacgc caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga
     5641 atgatcactc cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat
     5701 gatactaagc caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca
     5761 aacgattcgc ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac
     5821 aatggaacaa cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct
     5881 tgaagccatg aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact
     5941 gaaccaatga tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg
     6001 aggaacaatc gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct
     6061 gtcaaggatc ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct
     6121 aaacgcgcac tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa
     6181 tgttgcatga tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt
     6241 cgtggcaaac gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct
     6301 gacaattgta cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat
     6361 gctactagtc gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct
     6421 tattttaagt gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg
     6481 ggaatatgct ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca
     6541 agatgatgta aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga
     6601 agaacagcat aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc
     6661 tgaaatgatc caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga
     6721 tgcttccata ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct
     6781 agcaacgaaa aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga
     6841 ggaattcgga ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga
     6901 aaaaaaatac ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa
     6961 aagcttctgg gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa
     7021 agcttttgat cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat
     7081 acttcaggat ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaaa
     7141 ggataaacat gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt
     7201 ttatcagttg taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta
     7261 gctcctagtg cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca
     7321 gtcatatcga tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt
     7381 tgattggctt ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct
     7441 aattcaccta attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca
     7501 ctattttttt cttcaacaag ttctttcact gttcggatac tgtcaccaat aacattagca
     7561 gggactcgca acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt
     7621 cccggcataa attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct
     7681 gcttgataat cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg
     7741 gttgatttag gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct
     7801 tcttccatgg atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact
     7861 tttttcgtta ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc
     7921 atgtttgcat ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc
     7981 gactcatcta atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga
     8041 gtagaaagtt ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta
     8101 taatattgat gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga
     8161 tagagtttcg ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact
     8221 aaataaagag aatccaacca atccaattca accagcattt gcttcattcc ttcaagaatt
     8281 tctctacctt cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta
     8341 tttcgagtcg taccttctgc tacccattgg tcacagtctt ctttttcttc tgtccataac
     8401 atgtacatca ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca
     8461 taaggattca aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct
     8521 gcgactttct ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt
     8581 ttcccttctt cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa
     8641 actttcacat ttggatgatt cgtatagccc agcgcactgt tgcgaatact gcgaacaggt
     8701 tcttgcggat gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat
     8761 aaataagtga tcatccagcg atagcgcata aagtttctcg ctgttttcaa ataaagttct
     8821 gttttgaatt cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac
     8881 tcatcgccaa attcaaaatt gaaatgaata ccgcttacca tctgttttcg cttcccgtat
     8941 tcttttgcta aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg
     9001 atctcttcgt ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct
     9061 ttcggtatag aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga
     9121 atagaatcgg taacaggggt gatcatctct gtttgtgttt cgctaaaatc tgtttggata
     9181 taggggtgat aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct
     9241 gcaagatcaa cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg
     9301 cgcgcattga catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat
     9361 atgaatctaa ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt
     9421 acaaataagt ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa
     9481 acaaggagct gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt
     9541 agtaatcctt tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg
     9601 attccttcac caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt
     9661 acgataccgc cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt
     9721 gtttctttga attgttttgc ttgaaccatc gcgttttgac cagtggttgc atctaataca
     9781 agaagtgttt cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct
     9841 aattcgttca tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca
     9901 tctgcctgct ctgtctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgcct
     9961 cctgcattgc cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg
    10021 attgctccag cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg
    10081 aactgatggg caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac
    10141 aaaataactg ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc
    10201 gcttcataga gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc
    10261 gcatttctga gtttcacttc ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa
    10321 cctacatctg caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt
    10381 cgaaaattgg caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa
    10441 cccttgtcgt acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca
    10501 cgcttgattt tatcaaaaaa tcccatttga ctccccctac tctaatacaa atttttggat
    10561 cactttggct acgccatttt cttcattgct ggctgtcacg aaatctgctg cttctttgac
    10621 gaaaggcaca gcattctcca tcgctacgcc tagtccagca tattggatca tcggtaaatc
    10681 attttcctca tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat cttttgccaa
    10741 taaagaaata ccataagctt ttgtaacacc ttttggcata aattccaaaa gcatacttct
    10801 tgttttgatg atctcataca tttcatggaa ttctgctggt atttctttga ttttaggatc
    10861 taattcttct tggtcataag cgattactgc cttattaagc acaaattcat ctgtaatatc
    10921 gcttatatcc gctggttgga attgcagcag cttattcaac acattgtaca aggattgctt
    10981 ccttggggca ctaggcagct gtaaaaccac attgtcagac aaaacatcta gtggcagatc
    11041 caattgctga gccagtttgt ataagcgttg gatatctgat acttccatca ctttcttttc
    11101 catgatttca ccagtatcat ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc
    11161 accttcatct accagtccta attcttgcag ataatgcgcc atcgcagcta aggggcgtcc
    11221 tgtacataga acgatcttga ccccttgctg ttttgcttgt gctaatgctt gtttattctc
    11281 atctgaaatc agcttttgtt catttaataa tgtaccgtcc aaatcgattg ctactaattt
    11341 gatcattttc gttcctacac tttcgtttcg ttgattttcg cctcttcatt gatatcttcc
    11401 atccgtacag aaacgatttt tgatacaccg gattcctgca tcgtcactcc gtacagtaca
    11461 tctgctgcga ccatcgttcc tttgcggtgt gtgactacga taaattgtgt gtcatcttgg
    11521 aaatcgctca agtaacgacc aaaacgtttc acgttggctt catctaacgc tgcttctacc
    11581 tcatctaaga tacaaaacgg aacaggacag acacggataa tggaaaagag caacgcgatg
    11641 gctgtaagtg ctcgttctcc tccagaaagc aaacttagac tttgcagttt tttgccagga
    11701 ggttgtactt caatctcaat acctgttttc agcaaatcag ttggttccgt cagtactaat
    11761 tcagctttac cgccgccaaa catatttgga aagacaattt tgaattcttg acggatagct
    11821 tcaaatactt ctttgaatct tgtccgcact tcatcatcca tctcatccat cgtttcaaac
    11881 aattggtttt tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt atgtctttca
    11941 ctgacttgtt cgtattgttc gattgaattt aagtttactg gtcccagttt ttcaatctgt
    12001 tcttttaagg atgaaacctt tgttctactg tcttcgatat cggttgtttc ttgatagtct
    12061 tgggaagctt tctcaaatgt cagctgatat tctgactgca gataggtcaa atgattatct
    12121 aaaatcattt cagctcggtc tttttggact tctaatttgg attgttccga cagtcgtgct
    12181 ttttgctcac gattacgctc tgccaagact gcttcaagct tatcgatctc cttttgcctt
    12241 ctatctcgct gctctttggc ttttgccaac tcggctttta atgtttctcg ctgagcagat
    12301 aattcgttaa tttgtttttc caaactttct tctgtgactt catgatcaga aaagtctgct
    12361 gtcaatgtcg ccaactgctt ttctatcgct tcttgacgtt cggttgcctc tgctttttgg
    12421 actcgggcac cgcggagctg gatctgcaga tgattaaact gctctttcaa gacagcaaga
    12481 tctgcttgtt cttgtgcttt ttgactttgt acttgtgctc ttctggcttc catctggtcg
    12541 ctttcttgac ttaacgattt gatttcttcg tcgatttttt gtcgttgcga ttcaagatct
    12601 gtttgtttgt ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt
    12661 tcacgtgtct caaaagttga gatttgcttt tctttttcaa aacgttcaag ttcatttatg
    12721 atgttttgta gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta
    12781 cgcaaaactt cttgttcttc ggctaaacga gctgtttctt gttgcagctc ctgaactttt
    12841 ttttcttgtg cttggagttg tttatctgct tcttcaaatt cactagtcaa ctgtttcaat
    12901 tcttgatttt gtacaaacaa actgccttgg tttcctcgtt tattcgctcc acctgtcatg
    12961 gatcctccgg cattcatcac atcaccttct aaggaaacca ctcgatattg atagcggatc
    13021 gtttgcgcga ttgcattggc actagttaag tctttggcta aaaggatcgt tcctagcagg
    13081 ttttgaacca cagtttggat ttgatcggga taactgactt gttcactggc aatccctata
    13141 aacccttcta cagctgctgc ttgtgtcaat atatgagccg gtaattgtct tggtttgatt
    13201 gtcgtcagtg gaaggaatgt tgctcttcct cctctttgct gtttcaaata cgtgatggct
    13261 tgccgagcat ctttttcatt ttcaacgatg acatgctggg ctgccccacc taaagctgct
    13321 tcgatagcta acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc tccgacaata
    13381 cccgaaagct gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag
    13441 ttttcttgga tttcttgaag gcttttctta cgagcacgta gctgctggac ttgattcatt
    13501 aattgataca ttttaggctg ttcttttgct aatgcttctt ggaacttttt cccgttttgt
    13561 tggatcattt ctaacttctc ttttgtttca gttaaagatg attttagctg cgcctgcttt
    13621 tgtgtcaatt cttctttttg taagatcaat cggtctactg atgcttctac ttccgattgc
    13681 ttggctaacg tctgcttgct ttttgctgtt tcttgtatat attgacgttc tagatacttc
    13741 agttcgtttc ctacagctgc ttgttcttgc atcaaatcaa catattgatc tcttaattct
    13801 gccaataatt ctttcgttga tttttggtat ttttctaatt cttgctgtgt ttttatgaga
    13861 tttgcttcag ctttttggat ctctgtttct ttttctgctg ctgctttcat caagctttct
    13921 tgaagctttt caaaatgttt aacctttttt tgtgcttctg ctaatgaagt ttgatattct
    13981 tgcgagcttt tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg tccttctgtc
    14041 tgcttcagtt tttccgaaag atctaataat acttgctgat ttttttcgat cagacgatcg
    14101 gcttgcgcat tttctttacg ctgcttcgcc aaaatggatt cttgttcttg gatcgactca
    14161 gatagtttgc ctaattctag attgaatttc gcaagctgtg cttgtttgtt gtcccaatct
    14221 ttttttgctg tcttgatttc agcaaccatc aaagagacat cggtttgagt cagtgtttct
    14281 tttagccgta aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt taactgttct
    14341 tccaattcgt ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc
    14401 tgttctgctt tctttttccg ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc
    14461 ccacgacggt cttccggttt actactgaaa atcgcttcta cttttccttg agaaatgatt
    14521 gagaacgatt cttttcctaa tccagaatct aagaacaatt cttggatatc ttttaatctg
    14581 cagctttgtt tattgatgaa aaactcgctt tccccagtcc gtcgataacg gcgggttaca
    14641 ctgatctcat tgtactccaa tggcagataa tgatcactat tgtctaaaat cactgtgact
    14701 tctgcaatat tcagctgctt acgggtatcg gaaccggcaa agatgatatc cggcattttt
    14761 ccgcctcgca gacttttagc agattgttct cctaataccc agcggatagc ctctgtaata
    14821 ttgctttttc cgctgccatt tggtccaacc actgctgtga cgctattttc aaaatcaatg
    14881 actgtcttgt ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct
    14941 ttccctcact tacttttaca gtgctgccaa tgcattttcc gccgcagctt gttctgctaa
    15001 ttttttcgat ttcccttgtc cagtgccaat caattgatca tctacataaa cttcgatcca
    15061 aaatactcgt tcatgagctg gaccttcttc attgatcaat cggtattcga ttgatacatc
    15121 acctgatttt tgcaagactt cttgtagttt tgttttatga tccatctcat gtgaaaaagc
    15181 acctgctttg actttaggaa aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc
    15241 ctggtcaaga tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg
    15301 cgtacgtcct cctgaattct cttctccctt gcccaacaaa acatattgat caaagtgaca
    15361 ttctttggca aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa
    15421 ttttccttct ggcaaatgag gatataaacg gaataaatac tgagaaacca tcagttctaa
    15481 aacggcatct cctaaaaatt caagtcgctc attatccgat aattgcagat tgcgatgctc
    15541 attcacatag gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat
    15601 gttatagttt tcttttagtt cttttgtcag ctggttgtcc attttccaca ttccttttat
    15661 ggatattttt tcgatacttc cttattatac tggttttttg tcaaatttga aatggttcgg
    15721 tcaaaaggta agtaaagctt taataatcct agtgattgac gagaagttgt gaaaaattcg
    15781 tttagtttcg agtatgaaga aaaaattttg tgaaaatggc tttccatatt tttgcataaa
    15841 tttgacttaa taccgagaaa ctggactttg aacaccgttt attggagaag tcatgaaatc
    15901 agcgccttga cctgaatatt agagaaataa acagaaaaat atcaaaaaac cgaaaaagga
    15961 catcgccctt tttcggtcat ttttagtgat tatgaacgtg aatctgctgt aacagaaaca
    16021 tttgcccatg ggtttggtac atcataagac caatcccagt ctttgacacg gtcgttgact
    16081 ggcaatactt cattacggaa caacgttgga atcgcaaatg cttcttcaaa tgcatattgt
    16141 tgccattttt gatatgcttc tttttggtac tcttcatcaa atgctttgct tgaactgata
    16201 tcgttgatca attttgtatt ttcttctgat tcataacgag tgtagttgaa cgcagcattt
    16261 ggtccccata gtccacttgg atttggatca gttcctgtat tccaagcagc ttggtaaaca
    16321 tcaatttcag gatcatcgtt ttctaattta tcatagaaag aattgaattc gatcagacga
    16381 cctgttgtgt attggacatc caaaccaatt tctttccatt gttggatata gtaatctgcc
    16441 aatggttgcg ctgtttcccc accggacatc gaagcaaagt tgattgttag tttatttcca
    16501 tctttgtctt ctcttaaacc atcgtcatcc acatctttgt agccagcatc atctaatagt
    16561 tttttcgctt tgtctagatc ttgtgtgaat cctttcatct catcatcatg gtatgactgg
    16621 aatactggag gaatcaatga agtcgcattg cttctcaaac ctgcatagaa acgttcacct
    16681 actgcattat tatctaatgc atagcccatt gcttgacgta atgatttatc agccattttt
    16741 gaatctggat tgtattccac actctttgta tctgcattcc atttacctaa cttgaatcct
    16801 agataagtat aagaagtttg ttcacggcct aagtttgtat agccagcaat ttctttatag
    16861 gtatcgtaag tatctgttgg catttggaaa atcatatcat ataatttaga gttcaatgct
    16921 tcagttgcac ttgccatcgg tacagatttc atcgtgatct ttttcaattt aggcgtacca
    16981 ttccagtagt gctcgtttgg caagtattct actgattctc ctgcaacgat attactcatg
    17041 taataagggc caaaagtcac tggattttta cgtacttgat cgcttgattc catatctttg
    17101 attgctacac cttctaacgt atgttttggt gctgcatatg accagatacc accacccgat
    17161 tgaagcatgc ttgggtttac ttctttatat tcgatttcaa ctgttttgtc gtctattttt
    17221 ttgattccgg agattgtgtc ggcacttcca tcatgatatt cttccatacc tacgatgtta
    17281 cggaaagtgt cgtcataacg gataccagtg taatctttat tcccaatgat ttcgtaaggg
    17341 aaaatcacat cgtccgccgt tacatcttca ccatctgacc atttgacgtt gtcttttact
    17401 ttgattgtcg cttttttcgt atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg
    17461 attctgaaat cgtcatcgtt tgcaaatagc ggttcatgag aaggagccat aaaagctgca
    17521 tcatatgaat cttggtaaaa ctcccactgg aacaatcctt taaactgact gtccgttgcg
    17581 accgcagctt ctaattcgcc gtcttcgatt gcatcatcat tattttttgt tgcaattgga
    17641 aaagttaatt cctctttgtt attgccgttg cctccagagt tgtttccaga ggtatctgta
    17701 cctccgcccc cgcatgctgc aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc
    17761 gttttcttca ttcctatttc ccccttcgct tctatactag cctaaccgtt ggcgcgcatc
    17821 tgcagaacgc ttgaatgctt gtccaacata attgatactc aacatcaaaa ctaaaataag
    17881 gattgatgcg ggtaaccaga tccattgccg attcactaag acttctccgt tacttgcata
    17941 accgatcaat gtacctaaac taggtacatt tgccggcaag ccgaaaccta agaaagtcaa
    18001 agttgtctcg ataccgatgt ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga
    18061 acttagattc ggcatgattt cgcggaacat gattttaaaa tcattggttc ccattgtttt
    18121 tgaggcactg acataatctc ttcttgcttc agataatgtc ttgcttcgga ataatcgggc
    18181 tttagctacc caataaaatg cactcatgat gaatataaag gaccagacat tgtatctgga
    18241 aataatcgat acgaagacga taatgatcag catgatcggt agaatcatga taaaatcaac
    18301 gatccgcatc aagatgttgt caatgatccc tccgtagtag ccagagatga tccctaatcc
    18361 aaccccgatg atcgaagtaa ggatcgtgat cgcaaatccg atcgtaacag agtttctagc
    18421 acctatgatc aattgtccta atacatcacg tccgccttca tctgctccca gaatgaactt
    18481 ttgtccttgt gtcgaaactg ttcctggtgc agcgtatttg tcgaagatac taacatacat
    18541 cactttgtct tgatcggtca gcatcgcacc gataaagatc actaataata aaaccactag
    18601 caaacctaat gaaaaaatag caattttatc ttttttaaat tctcgagcaa tcattcgtat
    18661 ccccatagga ggaatacttt cttgaactgc tgtattgttt ttttcactca tcgatccatt
    18721 ctcctttctg attggtttta ttgaatccga atacgcgggt caacgatact catgatgata
    18781 tctgaaagta atgtccccac tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc
    18841 acggtatagt cccgttggcc gatggaatca ataaatagtt ttccgactcc cggataacca
    18901 aaaatctttt cgataactac agaaccagca atcaacgaag tgatttcata ccccatttga
    18961 gaagccatcg gcaacgctgc atttcggaaa atatgtcgac tgaatacttt attcgtcggt
    19021 acccctttgg aacgggctgt tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt
    19081 cgcagatatt ggattgtgac ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga
    19141 atatatttca aacggtctat ccattgagtg ataaatccac cttgcaaacc tagtgtttgt
    19201 gatccacttg ttgggaacca gcctaaacga taaccaaaaa caaatagcat gatcaagccg
    19261 aagataaata aaggaacagc aaaactaaag aagttgtaga ttacgacaat cttatccaag
    19321 attgaatttt gataccggcc agcaagcatc cccagaggca cagcaatcaa ataagtaagg
    19381 acaactgtca agatcgatag atataaagtg ttgatgatcc gttcgccgat caagtcagca
    19441 acagagcgtt tgtataagaa actttgacca aaatcgcctt gcaaagcatt tccgatccaa
    19501 cggaagtact ggacatacca tgggtcattc aagccagctg cttgtcgcat ctgttcgatc
    19561 actgccggat cctgatttgg attgatcaaa ccggtaaatg gatcccccgg catcgcttgg
    19621 gctaataaga agatcagaac acttaaaata ataacttgcg ggatcatcaa taaaacacgt
    19681 cgcagaatcg ttttccacat tagcttgcac ctccatcttt caacgccact ttgtgtgtag
    19741 ttgtgatcgt acgcaaatcg tagactcgac cagtcgcatc atagtattct ttttggtttt
    19801 ggatatactc ttgttctact cttcgtcgat tttctttgtg gctttcccta ttttctacat
    19861 cgatttgcgg aatagctgaa agcagacgtt tcgtatagat atgtcgcggg tcattataaa
    19921 tgtcttctct tgtaccgatt tctacaaatc gacctttgta cataattgcg atattgtcgc
    19981 acatgtgttt tactacacct aaatcatgtg aaatgaataa atagctgagg ccgtattctt
    20041 gctggattcg tttcatgaaa ttcaatactt gcgcttgaac agatagatcc agagcagata
    20101 caggttcatc ggctacgatc agttttggat tggtagcaac agcacgggct actcctagac
    20161 gttgacgttg tccgcctgaa aattcatgag gatatttata tagcgcatct tccggcatcc
    20221 caacgatatc tagcagtcct tttacctttt tcttttcttc ttgatcgctt aaccgttcaa
    20281 aatttcggat gggttcagca atgatatcca acactcgctt tttaggattc aaactggaca
    20341 ttgaatcttg gaagatcatc tgcacatcct tgttataacc catttttttg cggttgcttc
    20401 gcttcgttac atcttttcct tcatacatga tttgtccggc tgttaccttt tctaatccca
    20461 caacggcttt tcccgtagta gattttccag agccagactc tcccactaaa ccatatgtct
    20521 ttcctttttc gataataaaa tccacaccat ctacagcata tacatgatcc gttactcgat
    20581 tgaagaatcc actgcgaata gggtaatgaa ctttcaggtc tttgattgtg attaattctg
    20641 tcatctacac ctctcctttt tcgtctctaa agtggaaatg cttatagcag gagcaacgta
    20701 ccaagtggtt cggtgcgact tcatgaagga taggctcttc ttcatgatcg cttgctggta
    20761 tccatggaat acgtggtgca aaccgacaac ctttacgagg catgtttttc agagaaggaa
    20821 cgacaccttc gatcacatgg agttctgcat catgatttcc ttcttgtggt atagagttca
    20881 gtaaagagcg tgtataagga tgcttaggat gatcaaacag ttcttttaca cctgctactt
    20941 caacgaactg tccgccatac atcactgcca ctcgatcagc catttctgct acgactccta
    21001 agtcatgagt gatcaagatg atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat
    21061 caagaatctg tgcttggatg gttacatcta gggctgtcgt cggttcatct gcaatgacga
    21121 ttggaggttt gcaggcgatt gcgattgcga tgatgacacg ttgtctcata ccgcctgata
    21181 gttcatgggg atattggcgt ccaactctcg caggattagg aatccctact tgttctagca
    21241 attctagtgc acgagcttga cgctgttctg cattcatctt tgtgtgatag gaaagtcctt
    21301 ctttgatctg gtcttcgatt ctcatgagag gatttaatgc agacaacgga tcttggaaaa
    21361 tcattccgat atcatttccg cggattttat tatagagcgt ttcgttcagg tcgattaaat
    21421 tcagatcgtt atataaaatc tcacctgtaa tttttgtgtt attaggatca agtaatccca
    21481 taatcgtagt agccaaggta cttttcccgc acccagattc cccaacgatt gctaaaatct
    21541 cgtttttgtc taaagtaaat gaaacgtcat ctactgcatc gtaatagtca tctttgagtc
    21601 ggaatcctgt atgtaggttc tgaacttcta ataattggtt atcggacaat cgaatacccc
    21661 tctctgcttc ctaaatggaa gtttttctta ataaaaaagg acattacaca gaataaatcg
    21721 ttttataaca aaggttat
//
LOCUS       DBKOIJ010000035        21309 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000035 DBKOIJ010000000
VERSION     DBKOIJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.035"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..808
                     /locus_tag="ACV5NJ_001003"
     CDS             <1..808
                     /locus_tag="ACV5NJ_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HHY6485114.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1161..2009)
                     /locus_tag="ACV5NJ_001004"
                     /pseudo
     CDS             complement(1161..2009)
                     /locus_tag="ACV5NJ_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2162..3274
                     /locus_tag="ACV5NJ_001005"
     CDS             2162..3274
                     /locus_tag="ACV5NJ_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HHY6485115.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="ACV5NJ_001006"
     CDS             complement(3352..3645)
                     /locus_tag="ACV5NJ_001006"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HHY6485116.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="ACV5NJ_001007"
     CDS             3813..4127
                     /locus_tag="ACV5NJ_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HHY6485117.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="ACV5NJ_001008"
     CDS             4148..6127
                     /locus_tag="ACV5NJ_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HHY6485118.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="ACV5NJ_001009"
     CDS             6140..6466
                     /locus_tag="ACV5NJ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HHY6485119.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="ACV5NJ_001010"
     CDS             6482..7000
                     /locus_tag="ACV5NJ_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485120.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="ACV5NJ_001011"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="ACV5NJ_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HHY6485121.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="ACV5NJ_001012"
     CDS             complement(8436..9941)
                     /locus_tag="ACV5NJ_001012"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HHY6485122.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="ACV5NJ_001013"
     CDS             complement(10033..10623)
                     /locus_tag="ACV5NJ_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HHY6485123.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="ACV5NJ_001014"
     CDS             10802..12448
                     /locus_tag="ACV5NJ_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HHY6485124.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="ACV5NJ_001015"
     CDS             12497..13216
                     /locus_tag="ACV5NJ_001015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HHY6485125.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="ACV5NJ_001016"
     CDS             13695..14642
                     /locus_tag="ACV5NJ_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485126.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="ACV5NJ_001017"
     CDS             14654..15574
                     /locus_tag="ACV5NJ_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485127.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="ACV5NJ_001018"
     CDS             15615..17072
                     /locus_tag="ACV5NJ_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6485128.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="ACV5NJ_001019"
     CDS             17417..18031
                     /locus_tag="ACV5NJ_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HHY6485129.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="ACV5NJ_001020"
     CDS             18024..19748
                     /locus_tag="ACV5NJ_001020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHY6485130.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="ACV5NJ_001021"
     CDS             19745..21196
                     /locus_tag="ACV5NJ_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHY6485131.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6870 a         3608 c         4315 g         6516 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac catcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tagaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgg
//
LOCUS       DBKOIJ010000036        20981 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000036 DBKOIJ010000000
VERSION     DBKOIJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.036"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..563)
                     /locus_tag="ACV5NJ_001022"
     CDS             complement(<1..563)
                     /locus_tag="ACV5NJ_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HHY6485132.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1376)
                     /locus_tag="ACV5NJ_001023"
     CDS             complement(750..1376)
                     /locus_tag="ACV5NJ_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485133.1"
                     /translation="MCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIAEFIGSFRG
                     NVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMNDGGSKPQL
                     TVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACV5NJ_001024"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACV5NJ_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HHY6485134.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="ACV5NJ_001025"
     CDS             complement(2912..3910)
                     /locus_tag="ACV5NJ_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HHY6485135.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACV5NJ_001026"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACV5NJ_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HHY6485136.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACV5NJ_001027"
     CDS             complement(4926..5657)
                     /locus_tag="ACV5NJ_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HHY6485137.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACV5NJ_001028"
     CDS             complement(5661..6926)
                     /locus_tag="ACV5NJ_001028"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HHY6485138.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACV5NJ_001029"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACV5NJ_001029"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HHY6485139.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACV5NJ_001030"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACV5NJ_001030"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HHY6485140.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACV5NJ_001031"
     CDS             12268..13770
                     /locus_tag="ACV5NJ_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HHY6485141.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACV5NJ_001032"
     CDS             13772..14509
                     /locus_tag="ACV5NJ_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHY6485142.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACV5NJ_001033"
     CDS             complement(14680..15873)
                     /locus_tag="ACV5NJ_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6485143.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACV5NJ_001034"
     CDS             complement(15952..16158)
                     /locus_tag="ACV5NJ_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HHY6485144.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACV5NJ_001035"
     CDS             complement(16151..17293)
                     /locus_tag="ACV5NJ_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HHY6485145.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17820..18440
                     /locus_tag="ACV5NJ_001036"
     CDS             17820..18440
                     /locus_tag="ACV5NJ_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6485146.1"
                     /translation="MKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIERGLNY
                     PAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGHYDNFF
                     DSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDDLYSMD
                     MFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACV5NJ_001037"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACV5NJ_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACV5NJ_001038"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACV5NJ_001038"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACV5NJ_001039"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACV5NJ_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HHY6485147.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         6174 a         4299 c         3725 g         6783 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt g
//
LOCUS       DBKOIJ010000037        20922 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000037 DBKOIJ010000000
VERSION     DBKOIJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.037"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            382..1821
                     /locus_tag="ACV5NJ_001040"
     CDS             382..1821
                     /locus_tag="ACV5NJ_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6485148.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2116..2334
                     /locus_tag="ACV5NJ_001041"
                     /pseudo
     CDS             2116..2334
                     /locus_tag="ACV5NJ_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2583..2798
                     /locus_tag="ACV5NJ_001042"
     CDS             2583..2798
                     /locus_tag="ACV5NJ_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485149.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3035..3271)
                     /locus_tag="ACV5NJ_001043"
                     /pseudo
     CDS             complement(3035..3271)
                     /locus_tag="ACV5NJ_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3458..4369)
                     /locus_tag="ACV5NJ_001044"
     CDS             complement(3458..4369)
                     /locus_tag="ACV5NJ_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HHY6485150.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4388..5392)
                     /locus_tag="ACV5NJ_001045"
     CDS             complement(4388..5392)
                     /locus_tag="ACV5NJ_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HHY6485151.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5389..7512)
                     /locus_tag="ACV5NJ_001046"
     CDS             complement(5389..7512)
                     /locus_tag="ACV5NJ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HHY6485152.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7517..9964)
                     /locus_tag="ACV5NJ_001047"
     CDS             complement(7517..9964)
                     /locus_tag="ACV5NJ_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHY6485153.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9951..10340)
                     /locus_tag="ACV5NJ_001048"
     CDS             complement(9951..10340)
                     /locus_tag="ACV5NJ_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HHY6485154.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10400..10903)
                     /locus_tag="ACV5NJ_001049"
     CDS             complement(10400..10903)
                     /locus_tag="ACV5NJ_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HHY6485155.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10916..11137)
                     /locus_tag="ACV5NJ_001050"
     CDS             complement(10916..11137)
                     /locus_tag="ACV5NJ_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485156.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11142..11279)
                     /locus_tag="ACV5NJ_001051"
     CDS             complement(11142..11279)
                     /locus_tag="ACV5NJ_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HHY6485157.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(11276..12460)
                     /gene="mobT"
                     /locus_tag="ACV5NJ_001052"
     CDS             complement(11276..12460)
                     /gene="mobT"
                     /locus_tag="ACV5NJ_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HHY6485158.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(12719..13009)
                     /locus_tag="ACV5NJ_001053"
     CDS             complement(12719..13009)
                     /locus_tag="ACV5NJ_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485159.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(12999..14141)
                     /gene="dcm"
                     /locus_tag="ACV5NJ_001054"
                     /pseudo
     CDS             complement(12999..14141)
                     /gene="dcm"
                     /locus_tag="ACV5NJ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(14217..14381)
                     /locus_tag="ACV5NJ_001055"
     CDS             complement(14217..14381)
                     /locus_tag="ACV5NJ_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485160.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(14374..15048)
                     /locus_tag="ACV5NJ_001056"
     CDS             complement(14374..15048)
                     /locus_tag="ACV5NJ_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HHY6485161.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(15126..16466)
                     /locus_tag="ACV5NJ_001057"
     CDS             complement(15126..16466)
                     /locus_tag="ACV5NJ_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HHY6485162.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(16549..16884)
                     /locus_tag="ACV5NJ_001058"
     CDS             complement(16549..16884)
                     /locus_tag="ACV5NJ_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HHY6485163.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(16894..17268)
                     /locus_tag="ACV5NJ_001059"
     CDS             complement(16894..17268)
                     /locus_tag="ACV5NJ_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HHY6485164.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(17281..17595)
                     /locus_tag="ACV5NJ_001060"
     CDS             complement(17281..17595)
                     /locus_tag="ACV5NJ_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HHY6485165.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(17708..20896)
                     /gene="fss3"
                     /locus_tag="ACV5NJ_001061"
     CDS             complement(17708..20896)
                     /gene="fss3"
                     /locus_tag="ACV5NJ_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HHY6485166.1"
                     /translation="MLSLAVQAIVGAIAPTITFADEITHPQTVTVELDLAHQYAVEGT
                     FSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKNPLPDMSDKAKLVS
                     VLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAIEAKINQAIDDYQK
                     KPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVNGNQLIITPNANSK
                     ESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVETEGSLKIKKVDKE
                     SGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTITEKSVPAPYTIDTT
                     PMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSLAGNTFAIRKDSPT
                     GEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELKYANQT
                     VALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTLFTAKDGKAVKWSE
                     AFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAPEGYSLDETKYPVS
                     IKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGFNDLTFKVSPLEGT
                     NEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQKITPLEIRSTFK
                     ENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNRLMLYDLPEEEDSL
                     TSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDVDATKAAQEKDEKA
                     KPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYENKEAFEAGDKPVA
                     KDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFDDVSITHDVLDGSK
                     EAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDTGKYPEGTKFTFAE
                     INYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVPSGSQESSPTVKTF
                     PQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         5922 a         4564 c         3660 g         6776 t
ORIGIN      
        1 agtataatcc tatggaattg aatatcatag caatgtattt gaaaagcaaa ttggaaaaca
       61 aataggtgca acaccataaa tatgaaaaaa ttgggaaaaa gtagaatgga aatttgctta
      121 cgtctcacga gatagctcac actattaatc tgaagaacaa tgaagtcgac ttttagaaat
      181 tgctagaaga aagtaaagcg aattatcact agttactata gagtaagttc aaataaagca
      241 tctccaactt acatggttac atgaggatat caaatcgaaa ttactcaagc ttcctatggc
      301 tagagtcgcg agaagttgtt gggcagtaga cataggttat attgttctta gctacacgat
      361 cattagaaag gaaatcctat tatgtatagt tatcaagaag atgcaaaagt ccaaaaaaat
      421 cgctggtgga ttttgattgc tatatctatg tttaccttca tgtccacatt ggatggcagt
      481 attgtcaata ttgcactacc taccatctca gatgatatgg gcataccaat gaatcaagcg
      541 gaatgggtag tgtccgttta tctgatgatt gtctgttctt gtttgttgct gtttggtaaa
      601 attggcgata gccgtgggaa aatcaaagta tttcggattg gtacgctgat ctttgtcttg
      661 ggatcgctgt tgtgtgggtt caatcattcg ctaggtattt tattgttcgc acgggtggta
      721 caagccattg gtgctagtat gactatggct acaaatgccg gaatcatcac ggaagtcttt
      781 cccatacatg aacgtggtcg ggcattggga gcaataggtg cgtttgtttc attaggctcg
      841 attgctggac caggtcttgg cggcttgatt ttgtcccaat tttcttgggc gtacattttt
      901 tggatcaatg taccggtggg aatcgtaacg atgttgattg ctgaaaaatt cttaccaaaa
      961 gatatcacca tgagcggcca tacaatcgat ggagggggct ttgctgccat tgcggctacg
     1021 attatcagct tttttggagc aatctttttg gggcaagaaa gtggcttttt ccatccagtc
     1081 ccccttgtac tgttcgtaat ttcagtgatt gcctttgtca gctttttaaa agttgaaaag
     1141 aaagcagcct ctccattgat ttctttcggt atttttaaga acaagatttt taccatgagc
     1201 ttgattacag ccttgttgat tttttcttcc aacttctttg tcaatgtagt gattcctttt
     1261 tatctgcaaa atgctcgcgg gttgagtcct agctatgctg ggctgttgat gatggtcttt
     1321 cctttgatga tggtcgtcgg ggcgccttta agcggctacc tgaccgaccg ggttggcccg
     1381 gagattttgg tattagtagg cttgggctta ttgtcggtaa cccaggtcat gtatatgttt
     1441 ttgtcagtgg actctccttt gtggctatac attgtcgcta ctgccatcat gggtgccggc
     1501 aatgccttgt tccagtcccc caataatacg atggttatga gctcggtcac tatcgaaaac
     1561 ttgggactgg caggctcgat gaattccttt gcccgaaatt tggggatggt cagcgggatt
     1621 tcgctttcta cgacggtctt atatcaagcg atgtctgcta agatgggcca gcaagtaact
     1681 aactatatcg caaatcggcc ggatgtcttt ttatatggaa tgaagattac ctttttaggc
     1741 agctttgttt tgtgttttgt tgcctttttc ctgactatct ggcgtatcaa aaaacaagga
     1801 ggtattcaga aaaatgtctg aaaaaaccac tgttgaagaa tttttggagg cctgcgtatg
     1861 ggtgagtggg atttccatgg atgatggaag atgaagtgaa cttctaaaag ccgaaaaact
     1921 caagtattat cttcaaaata ctagtgctca atgagatgct tgatttgatc acaagaagac
     1981 tgcgaaagcc gctggcaacc gcaagctagg tatcccaccg atggactacg ttttagaggc
     2041 tactggttat gaatacggag ccaacgctct catctgcata agaatacatc acccagatta
     2101 tgtaaagatt ttcgatactt ctgtcaaaaa atcattaaga tagataaatg atttccgtct
     2161 agtcaaattg gttcagaatg tagacacaca gatggtaaga agtcactcac aattcgagaa
     2221 tggacttgtc ctatttgccg tatacatcat aatcgaaacg tcaacgatag tatcaatatt
     2281 ttgaccaagg gctatgaata caagcattgc cttagaaaac atcaagaacc ttaggaacaa
     2341 cggggatagc ttggttaata agagataccg ctgtttaggc ttgcaagtat actcttttat
     2401 caagaattcc gtggctttag tcatgagagg cttaatacta tgaaaatttt ctttagcatt
     2461 tttgatttag gttccttttc taattataat aagatgacca ctacagtaac ctactgaaat
     2521 atataaacag ccactgtagt accattttca aagtagtcta tgagaggaga atcttcattc
     2581 ttatgaacgc tggaattgcg gatatgaatt taataaaaaa aacccttaat gattttactt
     2641 ccaattcaat atcaaaaggt actggtatca atttaagcac aataaaaaag ctgaaaagtg
     2701 gtgaaagatc agtggaaaag ctgaatcttt tagatgctat aaaaataacg gaatttgcta
     2761 tgaaaaatgg aaaagctgag attgaaatat ggagataatt atcgtctagt gaattacttg
     2821 ccacttagct gaatttaacg atccttggtg cttgaagggg gagattcttg ggaatagagc
     2881 atacttgcaa gcctatgtct ttactaacaa cgatatctct tattaaccaa gctatccccg
     2941 tagttctaag gcttttactt ttatctaagc aataatcgta ttcaaaggaa atttttgacc
     3001 cttagaaagt ctcattttat cagacgtgag cttaggcatt gctgaatttt gttaattcgg
     3061 ttttaggttg tgctttgagc ataatatgaa agtattcttt atcacgattt cattcatcta
     3121 aggtaatgtg atgtgattct gaaattcttt caaaggcagt ttttgcataa gctgacatta
     3181 catcatcaat gacatgttta caatgtttca ctactaaaat aagggggtaa ccaagaagaa
     3241 aaactgaatg attatttgta tctaattcca tggtatatca accatttcct atatagactg
     3301 attatgacat ggattaaaat aaaaaggcac tatgtcttaa ttgtcggttt tcgagttacc
     3361 gaatggcatt catcacccac ctatagagga tgggcgactt ctgcctgaaa taagttacac
     3421 tacttataac ttctaataaa attagattga gtcattatta tttaacatca acgattttcc
     3481 agtttccatt tttttgtaat actaatttgt actgaaagta gctagtggtt tttgatacat
     3541 tatcaaggta ttttatcttt gtactcacta gaaaattttt tccgttttgt agataaactg
     3601 gattctctaa ctccgaaaac tttaagtcaa tattgattgg tcgcatcgca ccattttcaa
     3661 cataatactc caattctttt tcagaagctg atggataaag tttaaaaaat gtttctaaaa
     3721 aatcggtgat ttcttccgtt gttttggcat caatttctga atcgttttct acttgttttg
     3781 cttcatattt tgccttgtct ggctttccaa tcattgttgg caaactagtt actaaatgat
     3841 tcctgttttc atcttcaaag attgtcactc gatatgcaga agaaattgat tgcgtctttt
     3901 ttccctctgt aatatgttgc tccactaaaa atgaaacgac aaattcttcc gagtgttttt
     3961 caacatctag aatttttaca gattgaactt cggaactcgt gggaatatct gctcgaacca
     4021 tatcttggat taaagcgaga ccgttttctg taaggtactg ttcgagatta ctcattctct
     4081 tttcaattgc ttctttgtta tttttccatg aaaaatattc tttcgcaaaa tcctctgtaa
     4141 aactttcaac gccagaagta ttcactacct tctcttttat aaccactttt tcatgagttg
     4201 tatgttgatc aatggcggtg aaatttttat aaacgccaaa agcaagacta caagacaata
     4261 aaagccaaag ggcaagcacc atttttcgat gcttacccac ggatacaact cgttcttttt
     4321 tcttttttgg gctcttttcc ttttgattcc gttcaatttt tatcttcatt ctttttcctc
     4381 ttttctctta tttgtatcgg ccagctccga taagatgctg ccgccaatag ttttccgtta
     4441 gattcgtcca ccccactgga tctcctgcat gaaacatgcg attctcccct gcgtaaatgc
     4501 ccacatgggt cacataagta ccggcatcat aggttgaatg aaagaaaact aaatcacctg
     4561 gtttggcatc ttttagatca atatgagtca tggcatcata ttgatcctga gccacccgag
     4621 gaagtttcag gtcaactttg gcataacacc attgggtcaa gccactacaa tcaaaccccg
     4681 tttctggact tgagccacca aacacatagg ccgttccttc atacttaaat gcctcatcaa
     4741 aaatttcttg gactattttg tcatcaaact gagttgtagt taggtattgc ttcactaata
     4801 aacagtaaaa catattgcca taattatacc gccacccacc attttcctta atcgcgattg
     4861 ggttggggta atcgactttt tgaccacctg atttttcctt tgaaaaagat tcagctaaag
     4921 caaaactatg cttcttcccg tttttcccga catgatctag atacccgcca ccatagttat
     4981 aggcctgaat aatactatct tgatcacacc ctagttcttt ggaacgctta accaaagacg
     5041 caaaatagag acagccttgt ttgatggatg cctcggtact taatgaatta ggtggtaatc
     5101 cggctgactc agaactttgc ataacgtctt ccattgtgcc accagattcc acttgaataa
     5161 tcgccaaaag aattggaata tactctgtta tatccgattc tttggcatac ttttccacca
     5221 ttgcacggtg tttcaatact tcttctgaga cactagttcc gccaggagaa gtgccagaac
     5281 taccatttcc ctcactgtct tcaccaaaca acaaacccac acacatcaaa atacccataa
     5341 aagagatggt cagaaaagtt aatgaaataa cacccacttt cttcaagttc attttttctc
     5401 acctgacctt gattttggac gatggttcat tcttttttgt actttttgat tggtctgctt
     5461 tggcagagtg gacctctgtt tcacttgttg gaaagaattt ttcttacttg ctgattggct
     5521 tttccctttt gaaatgattt cttgactagt tagttttcgt ttgatttcgg gattcagatt
     5581 tgctctcaca atccgctttg gtgcttcttt tttcagcatc atatctttct gaggttgagc
     5641 tgctttatct attggaatat tcgcccttct ttttccctga cgatttgttg cttcactcag
     5701 tatcttgcgt ttttcgtcca ttttcgcatt tcttcgttgt cgttgttttt ctcgcaaatc
     5761 cactcgatcc gctttttctt ggaacagtcc gcgcttgaat tcttctggtg cttttttcga
     5821 cttttcgatt cctttatgga gattgtattt caaattcgtt ggcgtattcc ttacctgatc
     5881 ctttacttgt ttagtcttat ctttcaccaa agcttgggta tccaaaactt tgccgatttt
     5941 tctcccagcc aaattcattc gggattgttt cttattattt gaaactggat tctccttagt
     6001 taagtcagat agtatttcgt gatcgttcgg taatcgtgaa ttctttcgta gagaagaacc
     6061 ggatggctta aatttggatt ttgattgttc ctttgccaac ttatttccga ctgctgcacc
     6121 accaccaaaa gccaaagctc gtcccacatt tcgattgagt tgccgcaatt ttcgattcat
     6181 taacatttgc ggttttcgca tgacgcgtct tcccattgac tggctatcat tactctggag
     6241 gttgaacata ctcataatgt cgcccaattt gaaatagata ccggcaaagg tcacaatttg
     6301 cagaaaagca accatgaaaa atggataggt tgccgaaata ttgaaaaaca tcgtggagat
     6361 gctaaatgca gtcgtaatca ccaaagtaac acccgctcgc atcataatcg tattgaataa
     6421 acgaataatc gccttctttc ctaaactttc ataagtcggc aacatggaca ataagaaact
     6481 aattggcaga aacatcgcaa agataatgaa gagaatttgc gagaacaaca taatccctgt
     6541 tagaagaaaa acaaagaagg aaatgccaat attgaacaat accaaaaaga ccacggtccc
     6601 taaacggttc atggtcttgg taatactgag attcgcattg tcattgtctt caatctctgc
     6661 cttgaccgct tcttcccgat ctttgccttt gttttcatct ggactcacag atagaatctt
     6721 gttgacccgg tcttcaccaa tttcttctat gttggaatca tcaaattgca gtagtaacca
     6781 aggttgttgg acttgaatcg aaaacaaact atcccgaatc aaatccacac tatcttttcc
     6841 ttggctttct gaattgggta caacgatctc ggttccaagg gtaagggccg cttcactaac
     6901 atctgagcta aagtcattga tttttgtgat ataggtaggc gcataagcaa tgaacgaacc
     6961 cgatagtaga aaaatcatca caaaattaat cacggctcga actgctttcg tggtttctcg
     7021 ttttaataat ccggtatacg ccacatacac ccccagagct aaaatcatca ataatagaaa
     7081 cccaaaataa aatccggagg tctggagtcc attttcagta ataccagcca atgtttggat
     7141 attctttcca atactttcag ccgtatccga aatgaaatct aatttgtaag cctcttgaac
     7201 cacataaccc gtggcgttcg acaaatacaa actcaccgtc caaatgaaat tagtaatggc
     7261 ataaagacca tacatcacgc ttttccctaa gccatcaccc caattccaag gcaaccaatc
     7321 ccaagagcta tccacaaaga aatctagttg atagttgttc agtgaatatt tggaatatag
     7381 atttcctgct tcaactgtat catcaactag ccctgtcgcc tcggctaccg taccaactaa
     7441 actcaataga actatcagag tgagtatgct aataaaaacg attcccacaa tccgaaaaag
     7501 tttctttttc atcccctcac tccattctct cttgaacagg tggacgggta tcaaaggcat
     7561 ggaagagttc ttcaaagact ggatgtacct gaaccacgcc tacccgacca tacaaatcct
     7621 gcatcaagca ttggccattt tccaaatctc gtaggcgttt ttgattgctt tcatcttctc
     7681 gatccaagcc aaagaattcc aaggttttct tgatttcatg aatatcggta ctgcgaaacg
     7741 caaacttcaa cccgatatta ttcttcaagc gttcatccag taaatcatca ctattttgcg
     7801 tgacaaagta aaccccggca ttcatagccc gcccagcacg aacgagctta ttcgataatg
     7861 ttttcccttg cgctacttga agaaaactcc acgcctcatc taagtctaca attttgaaga
     7921 tttctcgatt actatggata aagtccaagg caaaagtgga aatgacgatt agcatggaaa
     7981 cactaagcaa ttccatggtg gtatattctt caaaagtggt ttctgcatct ggtaaaacca
     8041 aatccgctac ttgaatgata tttaactgcc tatctaaact aatcgactgg gtaatcgaac
     8101 catctgagaa aagcaaatgg gcaaaatcat aatcggtaaa ggactcgatg tgatccgcga
     8161 tattttccgc aactggtgag ccttcttttc gaagctcatt aattacttgt aataatcccc
     8221 gttgattgct ttgggtaacc gtccgaatgg ccttacgcaa cactggaaac ttctccccat
     8281 ctcgactaga aattcctgtt aaaaaggtca gaatatcaat tgctagactt tcagaatcct
     8341 tctttttctc cataatcaca taaggatcaa gtaatccttg attttttggc ttactagtta
     8401 agttgagaat attgatttca tccgcaattt ctggcaaggt ttctttccag ccaccccgtt
     8461 ccccttttgg atcgacaatc acagcttgtc caccaaaaag gacggcgtaa taaactaaga
     8521 gattgttact aaaggacttt ccgccaccga gtgaaccgag gaatgccgct gctagcgcat
     8581 tggtaactga acccttgacc ccttgtgagg ccaatgccgg tttcaggtaa acattgcgcc
     8641 ccgtatctac gttataacca aagtaaatgc cttccaactc ccctaacatc tgagtggcgc
     8701 caaaaccaag tccggcgaga aaatcagacg taacatactg gatataatca ttcatgtatc
     8761 gcttactaga aggtaagaat tcttcatgta gccccatcat atcgccaaaa ggccggacta
     8821 gttttacatt ggtatcatca taaaaatcta gcacttcatc acaccggcgc tttagttcat
     8881 ctactgattc ggcacttact cgaaccacat agctcagttt gtacatggat tctttggact
     8941 gatccaaggt agtttccaat tcatctacgg aatccaatgc atctaagaca ttggaatttg
     9001 tttcattgtc agattgatag gcatggttgt ccaagtcttt taattccttt ttcttattgc
     9061 gaacggtagc aagtgccttt ttgttcgtca caatctccac attcatacta gtgtcaattg
     9121 ggaatgtaaa ttgttgttgc tggtaataga aaagttctga ggagggaaac tccatttccc
     9181 ccacaattgt attaattgtt aagtacgcta catatgattc atgattctca tgttccatgc
     9241 ataagtaccg gggcttttct tcaatcaaac aacgactggg acgtaataag tcgtaacgtt
     9301 tcaccaaagt ttccgacttc agctttttct ttggtagctg aaattcatat tcttcaaatg
     9361 gtgtaccctt ttcgccatag atatgttcaa tcaaatacat cagatcactc ggtgtgactc
     9421 gtctgacttt aaatcttcga gccaatttac tttccattag tttttcaagc ttggtatatc
     9481 ggcgaatttc ttcattggat aaggaaacaa aatcgcccat caaatgatga ttcactccat
     9541 agacaaattc ttgaaagccg gagaagaaag atttcttgag attctttaga ttgacctctt
     9601 catctgtcgc aatcagtttg aagccaataa aaaagcgata atcaatctga ctatcgccaa
     9661 tcatggatac taatgcctct gtctgtaaat caattcgttg tttcgctact tctgttaatc
     9721 gcccggtaat ttcctttttt gatcgttctt gcgtggcacg aatactactt tctgtggcaa
     9781 tttgtaaggc atgaatcttg ccttcacgat tttgcgcaat caattgacga aagttatcgt
     9841 ggacttgata tttctgttca ggtgacaaaa aggagtaatt gtaaggtatt aactcatagt
     9901 aggcaaaaca ttctccctct tgattaaaga caagattatt ttcaatatac ttaatcgggt
     9961 acatagttca cactccttac cagagtaatg ggttcgtccc atcgtttctt ctgttctttg
    10021 actttctttc ctgcgaaagt gaccttaaat cgtaagtgat agctaataaa agatcgtaaa
    10081 aatcccaatg gtttcttacc gtcaaacgtt ttttgcgaca tgaaccaagt aactgccacc
    10141 ggtataccta agtattttaa aaaggccccc tctatcatag atagtggagg cacatgggca
    10201 aatacgatga caaacagcaa ggataaaaca aaccaagcca tctggttaaa agtcacagga
    10261 aaaggcagtt gaaagtcatt aattgcataa atgacctttt caactgccca aatactggtg
    10321 tagcttttta ttttcttcat caaatcattc ctttaaaaag aaggatagtt tagaaatcaa
    10381 ctacccttca ataattttgt taatatggat actcaaaaac acctcgactc gttaccaaat
    10441 aattgcctcc aatctccaag tctctaccac acgcatcaaa atcaatatac gtcttcaagt
    10501 gttctggtac ttctccaaaa tcaccagctt ctacaatcaa gaaatatgct acatcactca
    10561 tatcatcgca atctggataa tagataatat catccacatg ctctaccatc tcctcaaaag
    10621 aattgaagaa agcatgttga atctcagaag caacttcacc aactggcgtt ccttcaagtt
    10681 cctccgctaa gccacacaac cgattaattt cctcaattgg cgtatactca tcaatctcaa
    10741 agggaagttc ataatcatga attgcatatt cctcgtattc gtcatttaac ccaattcgtt
    10801 ctttcacttc atcaaaatcg acaggtgggg taaaccacgc ccccaccaat tcaccttcgt
    10861 tatatttccc gaggttcgca atataaacac gcatctgttc catcgtgctt cccccttacg
    10921 caccgacaat tcggttaaac aaactgagta agacatcctt caccccagaa gcattaaaga
    10981 ccaatccgac cgcaatcaaa gcaatcacca aaaagccaat cagtttggaa aactcccgct
    11041 taaatcctaa aaacaaacca atgaccacaa ttgccagtaa gactaaggat tgggcattgg
    11101 ttaaaaacca ttgatataaa ttttgtccga aattcatctt gtcactttcc tttcttttcc
    11161 attgctcgat ccgctgctgc acttgcattc atcaagcaca tcacagtaac accaagcata
    11221 ctgccaccaa ataccaaaaa aatatctttt acaattccca tgacgattcc tcttttcata
    11281 taatcaaatt ttcaattgcc atactttgtt gctccaaaat ctttttatgt cggtctgata
    11341 agcgtgcatt ctgtaccata tcctggatat aggttgtccg attcaatcga tccaactcct
    11401 tagccatttt ccacgtaggg gcgacttgtc ggcctagcca attcaaggtt cgttcaatgg
    11461 tatacggttt tggctcggaa gttaattgaa tctctttccg attccgtcca ataaaacgcc
    11521 cccattgttc attcaagggc cagttggttc gccttttacc ttctacttta tccgcaaatc
    11581 gcaagtaacg attgataatg gaaaacgtgg tgctctcaat gctacgacct tttaacaaat
    11641 cttgaatcgc atgaaaagca cggtcatttt tcagtcgaat ctcaaaacga tttttcgtct
    11701 cagtttggtc taatggaatc cctaacttga tatattgttc atagtctttt tcatacaaac
    11761 agaaataaac ctcactcttg agactgccga tatataaggt attgcccatg ccgtcctttt
    11821 catcagcctt taaaagttcc ccggaacgat aacttttaaa ggtacgaaac aaggagatac
    11881 attcttcctt ctgacatttt ttcgttagct caggtatatc caatagcccc actcgatcat
    11941 tgattgccaa gtccaagcgt ttcatcacgc caccggcctt tagacaatcc tcgaaaaagt
    12001 cgtaccagct acgcttttga gcgagtaaaa aagtttcaaa ttgtcggcaa cctcgcccct
    12061 ttaattctaa aagcacacct ttatcttctt catgagacaa catcaccaca atatcaccca
    12121 tgacatactg ttcttgatag gagtaaaagg cataatcttc atggagcata tactcaaatt
    12181 tcaaatggag aatttcttga ataatttttc gtgcatccgt tgtgggaaag cgaatcctta
    12241 catagtcaaa cagtaacgtc aaaggcaaat cgggattaaa gctttccagt tggtcaaaaa
    12301 ttttcctcat ggtttttata gatgcggtta cgccaccatt ttctatctga tttaagtacg
    12361 gccgactaag tccacaagcc gtagccaatt tattttgtga aacgccgtat tcgttgcgtc
    12421 ggtcttttaa tagccgataa tctaaattcc gttgtgccaa gttctcacct ccaaattcaa
    12481 ggtgttaact ttttcaaatg agttaacacc tatacaaatt acttactatc tttgatacaa
    12541 caagcattct taatggttgc ttacttattt tttgtgtttt tgtacccccc tgttagatac
    12601 gggggtgaga catcatggtc ggctaacgcc gtccatgaac aggcacagca ccacggagga
    12661 tttctgcttc gcgaatcctc cgcggtcagc gcctaacagt cagttgtgtt ggtttaggtt
    12721 actcatctgt aataccattt acttccaact ctgctttcat ttctaacaag tattgacgaa
    12781 tatctggttc ttttgcttgg tccgcaatcc gctcaaattc tgcaattact tgatttattt
    12841 caattcgagc atccgtctcc tcattcatta aatatggatc gccagcgact ttgacaccat
    12901 tcttgcgaat atcaaatttt aaccgtcgca attgttcttc taattctggg tctgactgaa
    12961 tctttccaat cacccaataa atttctccaa tcaactcatc ataaaccatg tacatccaac
    13021 tcttttaatc ttctggcaat atcaaaaatg accggaactg ttacagagtt tccggcttgc
    13081 ttatacaact gactatctga attgacttga gaatctcgat caaaagccca atcgggaaag
    13141 ccctgaagtc gccaacactc tctaggtgtt agcttccgaa tttgatactg atccgtgacg
    13201 atcgcttgtt ggtctcctgt tagtaaggta tgaacactct gttttcccag acgacctcgg
    13261 cgacttgtgg aattaggaaa agccagattc acactgtctt ctggcttcac ctcttgatat
    13321 tctgtttcat tttttcgaat catgattttt gtaccacctt gtttcgttga caaggtgggg
    13381 gcggttccag taacttcata aactcgatta ccttgttcaa actttcccgg taatagtcct
    13441 gctacagaga ttccatccct tgctggcagt gcgattttga ttccttcatc acctgttaag
    13501 gttggcgcta cgccatctga accatagact tgtccaccca ttcctcgatt ggaaggattg
    13561 atatttacca aatttctgat gttttgatca gatgttgaag acggtccaat gataggaaat
    13621 acttgttcgg tacatgcgtc tcgagaatgt ccgataagga atatccgctc ccggctttgg
    13681 ggaatgtagg cctttgagtt aagcacctac cactccacgt cataccccaa tccatccagc
    13741 gttcggagga tcgtctcgaa cgtagcccct ccttcgtgat tgagcaatcc tcggacgttc
    13801 tcaaggaata aaagcttagg tcggagaaga gtggtgaacc gagcgatttc aaagaataaa
    13861 gtacctcgag tatcggaaaa tctttgccgc tttcccgcaa ttgaaaaagc ttggcacgga
    13921 aatccgccgc aaaggatgtc cactggtccg agggattgaa taaactcatc tgatacactt
    13981 gtgatgtcat gcatttccac ctccttactt gtatcatgaa ttgctttata actacgtcta
    14041 gcaaatttat caatttcaca aaaaccgata caatgatggc tcgcctgttc catacctaaa
    14101 cgaaagccac caataccggc gaataaatct aaaaatctca tacattacct cattcattgc
    14161 aaagaaaaaa gccaagaaca ttttgctctt ggctcataca ttattaattt gtcctactat
    14221 tttttattta atccaactaa tttccgttgt ttttcattgg cctgatcggc tacatattta
    14281 atcaccgttt tgaactcttt tgcagtagca ttttccgata aatccgcaat gctcttattg
    14341 tatttcttta caattttcgt tacttgttct ttctcagtca tgtaattgaa cctccaaatc
    14401 tttcctacta taccctagtg gtaaaaataa ttctagatta tccgtagcat aatgaacttc
    14461 ggaaatatta ttcgtataat cttttattat gatatgaagc aatacatctg gatgtttact
    14521 ctttactttt gcaatgttct ctcttgattt gaaaaaagcg ttaataaatg cctttttcgg
    14581 cacatgacgt ccctctgcaa tttcacgttc ttgtgtaaat ttccaagcga ttttagggtc
    14641 ttgataaaca tagaaaatcg tcactcgaaa atgttttttc aacgcccgcg agatattttt
    14701 ttcagcactt aatattgcaa aagttgcatc cagaataaaa gaatagccat gatctaagac
    14761 atatctaaat gcctgctcta ccaaccaaga agatgctttt tgaaagagac tagagttttt
    14821 tccattataa tcaggaaact gggtgcgaaa gtaatccgca tcaataatca catggttatc
    14881 ataattctca gctaatccaa tggcaacctc tgattttcca gccccaggac tccctgccat
    14941 aaaaactgcg tctcgtccaa cttcggggga tttccctttg ataatataag aaataaactt
    15001 atccttattt gcttttgcat actctaaata agaatcatta ctttccatat taattatccc
    15061 ctttcttcct actatataaa agtattatac gttacttgaa gtataaagtc agtattgaag
    15121 atcactcaat cattcatctt ttctaattta ctaattgaat ctaagaaatc ataccctttt
    15181 ggtacaagtg gcgtataaaa ctcactaatc acactgccac ctgtatccac ataacctcga
    15241 cctttgatgt gcttcataaa gaagtttttt tcgacctccc caaacatcat ggaatatccg
    15301 agttcactca tacgacccaa agccacgcga aagttgaatt gatcgcggat tccgtctcct
    15361 aaatatttcg catccggcct ttggcaagcc aaaatcagaa agaaaccaga ttgacgaccc
    15421 aacatcacga tttgtttgag cttattcaaa atcaccgcac tttctttcgt cgttaacatc
    15481 tccatataag ccacgtattc atcaaagatt aaaaagttgg gtggaagtcc aagataggca
    15541 taattctctc ctgttttgta gttagacatt tctttcattg ccttgctacg agccatcata
    15601 cgttcataga aatcttccac acaagcagaa atttcttcct tttgtgaata aacatgaggc
    15661 atcaccgtac ctaaatcagc taaatctgca tttttgggat cgaggataaa caattctgca
    15721 tctgacttca atagtgcttc gataatggtg aggaggaaat aggtcttccc tccacccgtg
    15781 ccaccagcga ttaacatatg gggtaaagaa tcataagccc atacttgatt tttcatcaat
    15841 cgcagagtcc cattttctgc cactacttcg tctatcccaa ttcgattggc gatcatatca
    15901 tacagtaaag tatactccac ataggagtct ttgagttcct tttctactaa ctcacaatac
    15961 agtccgcttt ctaacttctt ttccaatttt aaaagctgat cttggtattt cccaagtgaa
    16021 atttgaacgc gaatagacag taatccatct ttcattcgat aatagatttt ggggaagtaa
    16081 ctgatagtct ctctggttcg gctactattc aaatctttga aaaagccttc actttgagtt
    16141 tgtttcactt cataccaatg gttctctaga atcatccgag ctagcttttg tcgatgccac
    16201 aagcttcgcc aatgatcaag aaaaaagtga atataaagca atatcatagc tcctgaaaaa
    16261 gcgagacttc caagcagaca tactaaatct tttatagacc aaacgatttc aatagtagaa
    16321 agtgatagct tagaaatatc aaattgtcta aactcttgaa aataaaagac gctcatccaa
    16381 ccgatgaaca gagcgagtag ccagccaaga acaaagtgat aaaccaagtg ctgatctcct
    16441 gcgcgtatgc gatgtccttt gtacattttg atggtttcca cctccaatcg aaaaagccca
    16501 ataggatttc attaggcaat ttcaaaaagt tatgctagtc atttactgct acgaggaggg
    16561 gagttgtttc gtttcaacca attgatttaa aaagccttca atagcatccg gcatctccac
    16621 accaatttct tccaaaatgg attctgcctg ccgacataag ttttcattta cttcgacaac
    16681 tataaagaaa gactcttctt ctcctaacca gtctagtaca tcagagtctt cttcactgtc
    16741 gctattttct ccaagtaaac cttgatgtat aatcagaaac attttttcat cagaggttaa
    16801 tgctttaacg agttgttcct cagtcagctc gccttttcca tattgatcga gacaatctaa
    16861 aatatgttca acattaattt ccataatctt cacctatttc tttctttcat tttggttcgt
    16921 aggatttcct tgattcggct gccgcttcaa gactaaatca tccgccttaa tgaaccaatt
    16981 aacggtagtt ccttctcgaa aaacataatt ggcaacggta tccacgattg gattgaccaa
    17041 ctctacctct gcatcataag caaactcgcg taagggtaca ttagctggga tactaacttg
    17101 aatcattcgc ccttgtgcag aggattttaa attataggtt cgttctttta cctcattacc
    17161 aattgttcca tcctcatttt gcttccgtac ttctcggcgt aatgcggaaa aacgcaactt
    17221 cccaaatgtt aaacctgaat ctacgacaat accttctgtt aatctcatct tcttttcctc
    17281 ctatgctttc actaaatcat caacatacaa cacgtaatca gtaaacccac gattctcaat
    17341 ctcatagcct ttggcaacca aatggggatt cacggctttt agcggttggt cttgatcaaa
    17401 atccttttca ccggcttccg cagctactac cacctcaata tcatcggccc gttgcttact
    17461 ggaatacaaa tgataactgc gaccaatcac cgtagtattc cgtccatatc cttctgttat
    17521 aatttctcct tcaccggcat aacttaactt tccaaaggtt tcagccatat ctggcacgac
    17581 aaatttcaat tccatcttct tttcctcttt tcattgttag ttttggatac aaaaatagcg
    17641 cgactacttt tttaggaggc ggtcgcgcac gttggttctt aggatttact tgtatcatct
    17701 catcacctta gttccggcga ttccagaaat aatagcccgc cgtcgcaaaa atcaaagtaa
    17761 aaccaatgaa cagtaaaaca ttggaatttt tctccccggt ttgagggaag gtcttcactg
    17821 tgggactaga ttcttgagag ccacttggaa cagttggtgt ttccggttgt ttcggcgtac
    17881 ttagtatggt tggaacttct tttggtgtta aggtttgaga tttttctttc aaatcttcat
    17941 tgtgttttcc attgatattc ccatctttat cgtaattgat ttccgcaaaa gtaaacttgg
    18001 ttccttctgg atattttccg gtatctactt ttttcgcaag cacagttttg gtgaattctt
    18061 tatcatttac ttcataatca atcttgccag atttccaaat ttctttgttt gtaccatccg
    18121 gtaataaagc atacagaatt gtttcgaaag cttcttttga gccatccagt acatcatgag
    18181 taatcgacac atcatcaaac atatccacca catcaccatg agtaaaggtc tgagaaccgt
    18241 tttctaggtg ggcctttgtt tggatagaaa catggtgttc cactgtacaa ctgacagttt
    18301 gggcttggtt attcaagcta acatccttcg ctactggctt atcgcctgct tcaaaagctt
    18361 ccttattttc atacacatag ttgaataaga caatagtttt atttaaaact tgttcggcgg
    18421 ttaatttatg ctgaattttc caagttcctg ttttctcttt gttcgccaac gttgcgcttg
    18481 tttctgcaat caccactggt ttggcttttt catctttttc ttgggcagct tttgtcgcat
    18541 ccacatcaat ggcttgcgct accacatacc agtcttcttt gatttcatgt agattgtaac
    18601 tcaatttatc aactagctcg gtagaatcca gagaagttaa ttctttgttt ccgtctttcc
    18661 aagtcgcaag agaagttaaa ctatcttctt cctcaggcaa atcatagagc attaaacggt
    18721 tcaaactaac agaaaatgct ttgttcgtca gcttctcgta aggaacggtc accgttttaa
    18781 ttggttgttt ttggtcttgt tcggtaatgg taaagacata ctcactcttc acaaagtctt
    18841 ctttgttttc cttaaaagta gaacggattt ctaatggcgt aatcttttga aaaccttctg
    18901 gggcttctac ttcttcaagt aaataatccc cataaggaag attttcaaac ttgccataac
    18961 catcaaaccc taattgctcg ttataagctg tggtcgcttc atcttcggca cctgtgattt
    19021 cattggtccc ttccaatggc gacactttaa aggtcagatc attgaagcca gtttcggtag
    19081 tgcccgcagc tgatccagca aatttaaaga aatcaaagcc aaagcgaatc acttgttctt
    19141 ttgccgtaac atttcgggta atcacggcat ttttttcgct atcatccacc tttttgatgg
    19201 atacaggata ctttgtttca tcaaggctat atccttcggg cgcttttgtt tcttgccaat
    19261 aatactcgtt gatagctaaa tgtttaacgg caacttggtt cttttcgtct aaagtcaaag
    19321 tgaccgtttc atcagaacct ttcgttccct tcgctaactc tggtttaaaa gcctcactcc
    19381 atttaacagc ttttccatcc ttagcagtaa acaaggtgta ttcagcaccc tcaaagactg
    19441 ccttaccttg tgctttatcc ccagtatctt tgtcttcttt cgtcaaagtg gtttcgccag
    19501 tgacttcttg attttgccct tttacgttac tggtaacaag ggctacggtt tgattggcat
    19561 acttcaattc gacttttaca ggtttgaacg tattcacgaa cccatgacta gctttagttt
    19621 cggtcacata ataggttccc aattccaacg cattagcaat ctcttttggt gtttccgcat
    19681 gaccgttttc atccgtggtc atttcttgaa cgatttcacc ggttggactg tctttgcgaa
    19741 tggcaaacgt attaccagct aaggaataat tgtcattcca aagatcactc cctgtttcaa
    19801 ctccgctttt atccagaatg atttgccctt tttcccgtgc gttttttgaa gtcacgtaaa
    19861 tggtttcgcc tgctttaatg gtggtagtca tgggggtggt atcaatcgta taaggcgccg
    19921 gtactgattt ttcggtaatg gttacttttg taccatgagg aatctcatcc aatgtagcaa
    19981 tgccttcttt gtcagtagtc acgtcttttg caggtaaagt ttttccaaag tctaaatgga
    20041 aaaccgttcc tggtacaaca tcacccgatt ctttatcgac ttttttgatc tttaaagaac
    20101 cctccgtttc cacatcaatt ttgattgtgt aggtattagg tttatcaatc gccccggcca
    20161 ttaaagtctg ttggccagcc tttttatagg cgacaggtgt tccggtacca gcagatttct
    20221 taagcgtcag cacaccactt tcttttgaat tggcatttgg ggtgattatc aactgattac
    20281 cattcacacg ataatcaatg tttgcggcat tttccacaac ctcatcgaac tctgacaaat
    20341 tcagattatt cgtatccgtc acagtagtcg attgccctaa tactgtttta gccgtactat
    20401 tatggaaggt tggtttcttc tgataatcgt caatggcttg atttattttt gcctcaatgg
    20461 ctgcgatatt tacggcacta ccatctgatc gttttattga gtgaagggta tacccattaa
    20521 cttcttgcca gatcatcttc tgtgccacaa tatgagtatc tgcatctgta cctacttttt
    20581 tccataaaac ggaaactaat ttggctttat cggacatatc tggtaacggg tttttctcat
    20641 agccaggagt gaactcattg taaatcggga ctcctggttc aatacagaaa atatcttgtt
    20701 taaagccgtt gtacactgca taatgcggaa cagtgacttc tgacattgga cgaccatcac
    20761 taaaagttcc ttcaacagca tattgatgtg ctaaatctag ttctacggtt accgtttgtg
    20821 gatgggtaat ttcatcagca aaagtaatcg taggagcaat tgctccaaca attgcttgaa
    20881 cagccaaact caaaatagtt aatacacata atttcttttt tt
//
LOCUS       DBKOIJ010000038        20905 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000038 DBKOIJ010000000
VERSION     DBKOIJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.038"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(18..1391)
                     /locus_tag="ACV5NJ_001062"
     CDS             complement(18..1391)
                     /locus_tag="ACV5NJ_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HHY6485167.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(1391..1786)
                     /gene="rbsD"
                     /locus_tag="ACV5NJ_001063"
     CDS             complement(1391..1786)
                     /gene="rbsD"
                     /locus_tag="ACV5NJ_001063"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HHY6485168.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(1814..2731)
                     /gene="rbsK"
                     /locus_tag="ACV5NJ_001064"
     CDS             complement(1814..2731)
                     /gene="rbsK"
                     /locus_tag="ACV5NJ_001064"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HHY6485169.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(2839..3852)
                     /gene="rbsR"
                     /locus_tag="ACV5NJ_001065"
     CDS             complement(2839..3852)
                     /gene="rbsR"
                     /locus_tag="ACV5NJ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose utilization transcriptional repressor
                     RbsR"
                     /protein_id="HHY6485170.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(3933..4532)
                     /locus_tag="ACV5NJ_001066"
     CDS             complement(3933..4532)
                     /locus_tag="ACV5NJ_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HHY6485171.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4718..5419
                     /locus_tag="ACV5NJ_001067"
     CDS             4718..5419
                     /locus_tag="ACV5NJ_001067"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485172.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5429..8140
                     /locus_tag="ACV5NJ_001068"
     CDS             5429..8140
                     /locus_tag="ACV5NJ_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HHY6485173.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            8591..10219
                     /gene="treC"
                     /locus_tag="ACV5NJ_001069"
     CDS             8591..10219
                     /gene="treC"
                     /locus_tag="ACV5NJ_001069"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HHY6485174.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(10279..11553)
                     /locus_tag="ACV5NJ_001070"
     CDS             complement(10279..11553)
                     /locus_tag="ACV5NJ_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHY6485175.1"
                     /translation="MIDDIFAINLYHFPFIPAFVYNEFIDKGGKRVKKETDTTNRLIR
                     FLGGKTSYYVLGIVILLALIIYFFHQISFIFKPVGIIIATILPPLLFALILYYLLEPV
                     IKRLSKHLSRNLSVIIVYVIILFLLGLGSIWLIPFLEEQAKTLISSLPDLFQDFQESL
                     RQFLEKTPFADSFNQFFASIDDVTSDIAGFIGNYWESGAQRISNIFSTVTAIFITLFT
                     GPIVAFFLLRDPQKFYQSVLAIVPPAFRTDFKNLTKIANQQLGSFLKGQIIASFILGA
                     VYWVCFLLIGLEFASVLAISAGLLCIIPYIGPFIAFFPGLIIAFQDSTFMAVKFVIVW
                     FAVQLLHGDLVIPRVMGDRLQIHPITILIVLLVMGDLMGIVGVIFGIPIYTLVKLLVT
                     FIFRKFKQRYNKFYGDKGEYENSSFSEEDYFK"
     gene            11712..13235
                     /locus_tag="ACV5NJ_001071"
     CDS             11712..13235
                     /locus_tag="ACV5NJ_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HHY6485176.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(13304..14032)
                     /locus_tag="ACV5NJ_001072"
     CDS             complement(13304..14032)
                     /locus_tag="ACV5NJ_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HHY6485177.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(14045..15325)
                     /locus_tag="ACV5NJ_001073"
     CDS             complement(14045..15325)
                     /locus_tag="ACV5NJ_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HHY6485178.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(15466..16068)
                     /locus_tag="ACV5NJ_001074"
     CDS             complement(15466..16068)
                     /locus_tag="ACV5NJ_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HHY6485179.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            16313..16519
                     /locus_tag="ACV5NJ_001075"
     CDS             16313..16519
                     /locus_tag="ACV5NJ_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485180.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            16831..17550
                     /locus_tag="ACV5NJ_001076"
     CDS             16831..17550
                     /locus_tag="ACV5NJ_001076"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HHY6485181.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(17631..18314)
                     /locus_tag="ACV5NJ_001077"
     CDS             complement(17631..18314)
                     /locus_tag="ACV5NJ_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HHY6485182.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            18532..20718
                     /locus_tag="ACV5NJ_001078"
     CDS             18532..20718
                     /locus_tag="ACV5NJ_001078"
                     /EC_number="3.6.4.-"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HHY6485183.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7133 a         3747 c         4074 g         5951 t
ORIGIN      
        1 tcagtctttt tcattaacta ttcataagac attaggtctt catcaaaccc gctaacgaga
       61 gtagtgatca ttgctacgac tagactaaca gccagtcctg ctaagtacca gaaaaaagta
      121 ccggaaatga atgcaggcaa tgtggtcaga ctaccaaata caaaagcgat cgctttcact
      181 tgggcaaatc ccattacagc tccaccagca gctgcaccaa tgcaagcagc aacaaatggt
      241 tttttcaatg ggatcgcaat tccgtaaaca gttggttcgg tcactcccgc aagtaaaatc
      301 gaagcaaaag cagaaccgga gatcgaacgg gtttttgaac gtttacttcg cagaaataca
      361 cctaaagctg caccagccat tccaaaattg gctgctcctg tcaacggaac gattcggtcg
      421 aacccataag cagagatatt actgatcatg acagggttta cagcccattg gataccaaat
      481 attaccaaaa tattccaacc accagcgatg attgcgccac tgatcggtcc gttaatatcc
      541 atcatccaag taattgcggc agctaaacct tcgccaaggt aaacacccgc aggcccgaat
      601 accattaacg tcaaaggaac cgtgataact aatgaaagta aaggtacaaa tactaattgc
      661 atattttcgc gaatatattt tttgaggaat ttttcaacaa aagaataaaa gaaaatacct
      721 aaaatcgccg gcatgacagt agacgtatac tgcatcagta ctacaggaat ccccagaaaa
      781 tcagtagctg ctttgccact gtctgaaagt aaagaggtca gattcggttc aatcagtgct
      841 gcagcaatga cagccgcaat atatccattc actttaaacg ttttggcagc agaaaaagca
      901 agaaagattg gcagaaaata aaagacggca ttcgacgcag cagacaaaat atgataagtt
      961 ccgcttgttt cactaagcca gccaatcgta gtcgctaaaa caaccagacc ttttaatata
     1021 ccagaaccgg caaacagcgg aatgatcggc gtaaagattt ccgatatcaa accaaatagt
     1081 ttttcgatcg gatttagctt ttctttcgga gctgaatgat cgatttgacg cgtcaattcc
     1141 ttgtaagcag gagccaatcc ctgatccatg attatttctg catatctatc gagtaaaatg
     1201 aaatacttaa tggaagggat ttgctgtttt aatttttctg cattggctac tggctgtttt
     1261 ttcaatttga tacgaagccg agtcgcacat ggaaggacag aatcaatatt gtcttttcct
     1321 cccaactgtt caatcagcat atgaatctgc tcggacagta ccatctgttc aggattcatt
     1381 gtcgtttcca ttagaagacc actccactca ctaatataat attggcgtaa ggtgtcattt
     1441 cgcctgtacg aataaatgca tgacagttat tcaactcttt tttcatttct tcatgtggga
     1501 taaagctaat cggtgtatct ggtaacaata atttgatttt ttccaatatt tccgggttca
     1561 ttgtctttat ttcttccgcc aattcaatcg attgtacctc aagttcttct aaaacgtttt
     1621 ctaaaacaga taaaaaactt ggaagaccgt ttgagacagc caaatctatt ttttcagtac
     1681 caaaggggac aggcatgcct gcatccccga tacttaattt atcaaagtgc cccatttgtg
     1741 cgattactcg tgaaatatct gaattgatta cttttccttt tttcatgttt gtttcttcct
     1801 ctcattgtta attttagact gctgattgtt ctaaagcttc gcggtaaggg atagaaggtt
     1861 gcgcaccata agactgcaca gcaacagaag aggctagatt tccataaagg atcgcttctt
     1921 tcaagttact aaggtctggc tgaagtgtgc tgcttaatgc gccaataaat gtatcacctg
     1981 cggctgttgt gtcaacggca tccactttaa aagcagggac gataccgtgt tctttttctg
     2041 tatgatagaa agcgccggca ctgcctagtg taatgattac tgttccaata cctaattcat
     2101 ggagtttttc ggctgctgcg accaaactgt tatgatctgt cactctgatt cctgtaatga
     2161 tttctgtttc tgtttcattt ggtataatga tatctgtctt ttcaagtagt tcagtaggaa
     2221 tcgtttcacg agctggtgca ggattcagga tcgttgtttt tcctgcgtct tttgcaatag
     2281 aaaaagctag gatcgtcgca tctaagctac tttcaaactg agcaataata aagtcactgt
     2341 ttgtaatcag ttgcttgttt tttaggactt cttgttcatg gaaagcatta tttgcccctg
     2401 catggatcaa gatactattt tctcctgctg cgtctaccat aatcattgct tgtccggttg
     2461 tggcgccttg gatttcagct atcccagatg tatcgatgtt ttcttttgtc agtagatcta
     2521 ataactgttg tccttcgctg tcagccccaa cgcctccaat aaaagatgtc cgtgcgcctg
     2581 aacgtttggc tgccactgct tggttggcac ctttacctcc gccagcgtga aaaatctcat
     2641 gcgcatgcat cgtttcgcct ggcttgggca tgttggttaa acgaagggta gtgtccatat
     2701 taatacttcc aattactgta accgtgttca ttttacaaac ctccaactat ctcatttatt
     2761 ttgatgggaa aattattttt taaaacgttt tagtaaaacg ttttaatagt tcgataaaaa
     2821 tatagcacac cttgaaagct atttcaaggg tgcagttgaa aatctttttt ctaatcgtac
     2881 aggtaatctt ttttcttccc attctttttc tgggaattgg atccgatcta acaataactt
     2941 ggcagatgtt tgtcccaatt cgaagattgg ttgtgcgatc gttgtcagtt tcggcttgat
     3001 atactcgcac atatctatat tatcatagcc aataatgctg tagtcttcag ggatgctttt
     3061 tccagcttcc tctaatccgc gatataatcc aaaagctaat tcatcattta aagcaaacac
     3121 accagttgca gcagaatcaa gaagttctgc tgtgatctgg tagccacctt gttttgagaa
     3181 ctgtgtaggt aagaggatca gttggtcgtg agggatttgg taaacatcaa gagcagattt
     3241 aaacccctca atccgagcgt ggacattttc cggtggattt tcaggataga caagggcgat
     3301 cgtttgatgt cctagactaa gcaagtgcat gcccgctagg taaccaccgc gaaaatcgtc
     3361 tgttcgtacg gcatcactaa atccttcaga ttttttctga tccaaaacaa tgaagggccg
     3421 gccttgtttt ttcaaatttt cgttgattgc atctgtagag acagcagaag tcgcgataat
     3481 gaatccatcc acacctcgac gtgtgagctc ggctagatat tcgatttctt tttgatgatc
     3541 agaatcagca ttacataata tcgtgacgaa attctgctta tacaagatat cctcaattcc
     3601 tcgcatcagt gtactgaaga atggattcgt gatatcagga acgagtacac caattgtttt
     3661 cgtttccttg gtaatcattt gtctagcaaa atagtcaggc tgatagccta attcttcttt
     3721 ggcagctaaa actttctgaa cagttttcgg actgaatttc tccgtatttc catttaatat
     3781 caaggagaca gttgtgattg aaacgccgga aagtttagcg acttctttga ttgtgatctt
     3841 ttttttcacc atgttcatac tcctatctaa atcctgtggt tattttccca tgaaatcaaa
     3901 aagaagtaaa tgaaaatcac gtgtgccagt acttatttgc tttgttggat agcttcgatt
     3961 atttgtttcg ttgagcgtac tcgtgcagtt ttcggaaata tcttttttat gaacaattga
     4021 tgggattctt tgtcacgatc actgcaagca tcttcagcaa gcacgatatg gtacccgtgt
     4081 tgaaaggcat ctaatgcagt agcatataca ccgtttgttg tcgctacacc gcttaaaatg
     4141 atagtttcga ttccacgccg gcgaagttga agatcaagag aagtaccaaa aaaagcactt
     4201 ggattgtatt ttgtgatagt caacatatta tctgtatctt tcaaagaatc cttcaaaagt
     4261 aaatcagtga attctggagg tacagaagca gtcattccac ccatatcagt ttgttgactc
     4321 aaataaggga aagtggagat gtctacgttc actaaaactt tcaaggcagc ggtacgtttc
     4381 aatgttctta ctaacaaatc attgtttgtc agaatagatg ctgcagtgtg tggagagagt
     4441 gtaccgttat ttacgatctc tttttgcatg tcgatcgcga caaaagcagt ttttgataaa
     4501 tcaaaaatca tattttcctt taaatcattc atgatgttcc ttctttcaaa taacaatgtt
     4561 ttttatgtaa gtaaatcata aatcgtttat ttgtcaacta aattaaagga ttcctttctt
     4621 tagtctagag aaattcagtg gtaaaataaa taagtgtgag aaaacgcatt cgatgcatag
     4681 atatagcgag aaatatcgtg aaaatggagg aatcagagtg aagtatgtcg aagtaaaaaa
     4741 tgaatacaaa cgatacaaaa tgggagaaac gaccattaca gcaaatgacg atatttcttt
     4801 ttcaattgaa aaaggcgaat tagtgattat acttggtcca agtggagcag gaaagtcaac
     4861 gattcttaat attttaggtg ggatggatac acctgatgaa ggtcagatca ttattgatga
     4921 cacagatatt gcgcaatttt cagataaaca gcttactgct taccgacgaa cagatgtagg
     4981 gttcgttttc caattctaca atctcgttcc taatctaaca gcaaaagaaa atgtggaatt
     5041 ggcaacggaa gtttctcctg atgcgcttga tcctgtagag gtattgaggc aagtgggcct
     5101 agctcatcga ttgaataact ttccgtccca attatcagga ggcgaacagc agagggtttc
     5161 aattgctcgt gcactagcta aaaatcccaa acttctttta tgtgatgaac cgacaggcgc
     5221 gctggatttt gaaacgggca agcaagtatt gaaattattg caaaacgcta gcagacaaca
     5281 cggcaatacc gttttgatca ttacacataa ttctgcttta gcgcccatcg ctgatagagt
     5341 cattcacatt aatgatgcta aagtaaggtc tgttgaatta aatgatcatc cttcatccat
     5401 tgatgagatt gtatggtagg gggattttat gaaaacaaga acatatctta aagcaggttt
     5461 tcgcgaaatt cggcaatcta aaggacgatt cattgcaatc atccttattg tcatgctagg
     5521 aacactttta tttgtaggtg tcaaaactgc tggacctgtc atgcaaaaaa cgatggatca
     5581 ctatgttggt acagcaggac tttctgattt gcaaatcgtt tctactggtg gcttgacaca
     5641 aaaagatatt gctcaagcag aaaagatccc agatgcaaaa gtagaaacag gaaaacagat
     5701 ctattattcc aatactggca aaaacgaagt gattcaaata ttttcttata acaaaaataa
     5761 caagcagaac aagctacaat taactgatgg aaaactcccg gaaaaaccaa accagcttgt
     5821 attggatgaa aaagcggaag atgaagggta taaaatcggt gatatttatc agattgattc
     5881 agatgaattg aaagaaaaag agtataccat cacgggcttt gtccgttctc cattgttcat
     5941 caacaatttg gagagaggat acgcgaacgt cggaaatggt agcgtcgatt attttgtcta
     6001 tttacctgaa tccgttttta aaacagatat ttattctgtc ttatatcttg attttacaaa
     6061 cgtaaagaat ttgaatacgt acagcaaagc ttacaaagat aaaatggagc aaaatcaaga
     6121 aaaggcagaa aagtatttac aagatcgacc cgaagaacgc ttagaagagc tgaaaaaatc
     6181 ggccaacgaa gaattagaac cggcacagca aaaaatagcg gatggcaaag cacaaacaac
     6241 gcaagcaaga acacggttag aagatgctaa aaaacagtta gcgcaacagt cagcaactat
     6301 ccagcagctt cctgaagcgc aacgaacaat cgctgttcaa acattatctc aacaagaagc
     6361 tcaacttgct gaacaagaaa ggcaattagt tgagaaagaa cgtgaactgg cctctgcaga
     6421 ggaagaattg aatcgaaatg cagaaaaaat tgaaaattta actaaaccat catacctata
     6481 caatgaacgt tcagaaaatg tcggttttca agaatttggt gatctgtctg atcgtatcgc
     6541 tgcgattgcc aatgtttttc ctgttttctt tttctttatt gccgcattga tcacctttac
     6601 gacaatgaca cgtatggtag aagaaaaccg cagggaaatc gggacattaa aggcgttagg
     6661 ttatacgaaa gttgaaatcg caaaaaaata tgcaatctat gcaagtctag cctcagggat
     6721 aggtattgtt ttaggaacga tattaggaac gaatcttttg ccaaggatca tttttgagct
     6781 gtctaacgag cggtatgata ttgggagtgc gcttattttt tatgactggc cgccgatttt
     6841 acaagcagct gccgcattct tcattgccgc atttggcgct gcaatgatcg ttttattcaa
     6901 agatttaaga gaacggcctg ctgcattgct tcaaccgaaa gcaccgaagc cagggaaacg
     6961 catttttctt gaatacatca ctccgctgtg gtcacgcctt agctttaatc aaaaaatcag
     7021 ttaccgaaat ctcttccgat ataagtcaag aatgcttatg gcgattatcg gaattgctgg
     7081 atgtgccggg ctgatggttg caggcgtggg attgaaagat tctctaagtt ctgtttcagc
     7141 caaacaattt ggaccgatca ttgactatca agcaatcgtt acattggatt catccacgga
     7201 agatagtcgt caaaaagcag cggatatgct gcagaagcaa gaaaaagtaa atgagatcct
     7261 agctattcat actcaaaccg tagaattgag aaaaaaagga catgcttcgc aaagtctgac
     7321 agtgatcgta ccaagtaaaa cagctgattt ggatcgtttt atccatttaa ctgatcttga
     7381 agagaaccca cgctccttgc ctaaaacagg aatcgctatt acgcaaaaag tagcagaact
     7441 atttgactta tctacaggag atcatatttc gctttatgat gaagatcaaa aagaatggaa
     7501 agtcgaagta aaagaaatcg tgaaaaacta tctaggtagc tttatttata tgtcccctga
     7561 atattatgct tcagttactg atcaatcgat gatggataat gcatttttgc tgcgtacaga
     7621 taagatgaca acaaaagaag aagaaatgct ttccgagaaa ctgcttgcca cagacgctgt
     7681 gacaaatacg tctttcatgt ccaaacaaat cgaaacgcaa gaagaatcaa tggcgaactt
     7741 agactcggtt gtcttaatct ttgtcgtatt atcaggactg ctggctttta tcgtcttgta
     7801 taatctaact aatatcaata tttccgagcg tgtacgcgaa ttatctacaa taaaagtcct
     7861 tggatttttt gataaagaag tcacgatgta cattgttcga gaaaatatca tctttactct
     7921 tttaggaatc atcggaggat ttggtgtagg atatatattg actgacttca tcttgcaaca
     7981 agcgtctatg gagaatgtca tctttccatt agtgatcaca tggtgggctt atgtcttatc
     8041 agcaggatta actatcgtat ttacggttat tgtgatgatt gttacccatt tcaaattaaa
     8101 gcatatcgat atgattgatg cgttgaaatc gaatgaataa aaacagaggt tgtgaaatca
     8161 gcggtttgac ctgagtatta gagaaacaaa cggcaaaata gtttttcata ttttgccgtt
     8221 ttttgagcta ataccgaagg acagctgatt gaacaccatg aatcagaggt tgtaaaaaag
     8281 ccgtcctgca tcgagcaaaa agaatactca aataaaatta gctcctcatg ttttacattt
     8341 gagaagactg attgccgtag ataaaagctt ttgaacacca tttatagaag aaacaagaat
     8401 attttgctat tttaaacaag aggatagaca aatacatgtc tgatctcttg ttttttattg
     8461 ggcaacagac ctcttagaat gtataaagat aaattttaca aacgctttcg gttcagttat
     8521 aataataaat gataacttgt atatataagt tgtggtaggt aataaatcag ttgaatggag
     8581 gtaaatatcc atgacattta aagacaaagt catttaccaa atctatccca aatcttacaa
     8641 agacagtaat ggtgatggaa ttggagattt aaaaggaatc aaagaaaaac tcccgtatct
     8701 gaatgatcta ggcatcgaca tgatttggct aaaccctatt tatccaagcc ctcaaaaaga
     8761 caacggttat gatatctctg attacacagc catcgatcct atctttggta cgatggagga
     8821 attcgaacag ctaatagccg aagcaaagaa ataccggata gaaatcatgc tcgatatggt
     8881 gctgaatcac gtttcgacag aacatgaatg gttcaaaaaa gcactagcag gggaccagca
     8941 ttatcaggac ttcttcattt taagagaaga gccaacggat tgggtatcaa aatttggtgg
     9001 aagtgcttgg tcgccatttg gagatacggg gaaatattat ttgcacttat atgatcggac
     9061 acaagctgac ttgaactggc ggaaccaagc agtaagagag gaactattca aagttgttcg
     9121 tttttggatg gaaaaagggg taaaaggatt ccgtttcgat gttatcaatg ttatcggtaa
     9181 agacgaacaa ttgaaaaata ataaagaaaa tgatggcaaa cctgaatata cggacaaacc
     9241 aatcacccat acgtatctaa aagaattgaa ccaagcaacg tttgggacgg attccactat
     9301 catcacagtc ggtgaaatga gctctacgac gatcgaaaat tgtatcttgt atacagaacc
     9361 tagtagagaa gaattatcaa tggtctttaa tttccatcat ttgaaagtcg attatgaaaa
     9421 tgggaataaa tggtctactc ctccatttga ttttgcagaa ttaaagcgac tatttcatac
     9481 atggggagaa aagatgagtg agggtaacgg ctggaatgct ctatttttta ataatcatga
     9541 tcagccacgt gcgctcaatc gatttgtagc tatcgatcag tatcgaaaac aaggagcaga
     9601 gatgctagca accatgatcc atttgaaccg tgggacgccc tatatttata tgggagaaga
     9661 aatcggcatg atcgacccag actattcatc gatttccgac tatattgacg tagaaagttt
     9721 gaatgcttat aagttactcc ttgaacaagg attcacagaa aaagaagcgt ttcgtcggat
     9781 caaagcaaaa tctcgcgata actctcgaac acccatgcaa tggtcagaca cagaacaagc
     9841 aggatttact acagggaaac cttggttaaa agttggaggc aagctagaag agattaatgt
     9901 agaaaaagaa ctatcttctg aagattctat tttgtcctat tacaagaaat tgattcggct
     9961 gagaaaaaca tatccaatcg ttgcacaagg agattatcat gcttatgaag caaatcatcc
    10021 ggaagtttat ggctttcttc gccaatttga aggacagcag ctgcttgtat taacaaattt
    10081 ctatgataaa gatacaagga tcgccatccc agaaggattc acagaagcca atattcttat
    10141 cgataattat tccgatactg ttcttcatca aagtctggag ctaaaaccat accaggcaat
    10201 tgctttgctg aaaaattaaa acaaaaacac aaaacgtcgt tcctacgagc tattctcgta
    10261 agatcgacgt tttgtgtttt atttaaaata atcttcttca gaaaatgaag agttttcata
    10321 ctctccttta tcgccataaa atttattgta gcgttgtttg aatttacgga aaataaaagt
    10381 aacgagtaat ttgactaacg tatagatagg aataccaaaa ataacgccca caattcccat
    10441 tagatcaccc atgacaagta aaacgattaa gatcgtaatc ggatggatct gtaaacgatc
    10501 acccattact cgaggaatca ccaagtcacc gtggagaagt tgtacggcaa accagacaat
    10561 gacaaatttg acagccataa aagtagagtc ttggaatgca atgatcaaac ctggaaagaa
    10621 ggcgataaac ggcccaatat atggaatgat acataacaac ccagcagaaa tagctaaaac
    10681 actagcaaat tctaatccga tcagtaagaa acaaacccag tagactgctc caaggataaa
    10741 tgaagcaatg atttgcccct ttagaaatga acccaattgt tgattagcaa tctttgtcaa
    10801 atttttaaaa tctgtacgaa atgctggtgg gacaatcgct aaaactgact gataaaattt
    10861 ttgaggatct ctcaaaagga aaaaagcgac aattggacca gtaaataaag taataaaaat
    10921 agctgtaaca gttgaaaaaa tgttgctgat ccgttgtgcg ccactttccc aataatttcc
    10981 aataaatcca gctatatcag acgtcacgtc gtcaatagaa gcgaaaaatt gattaaatga
    11041 atcggcaaaa ggtgttttct ccaaaaattg tcggagactc tcttgaaaat cttgaaacaa
    11101 atctggcaaa ctactgatca atgtctttgc ctgttcttca agaaaaggaa taagccatat
    11161 gcttccaagc cccaataaaa ataaaataat cacataaacg ataatgactg acaagttcct
    11221 cgaaaggtgc ttgctcaaac gtttgatgac tggttccaaa agataataca agatcagtgc
    11281 aaacaataat gggggcaaga tagtagcgat aatgattccg acaggcttga atataaacga
    11341 gatctgatgg aaaaaataaa tgatcagcgc aagtaagata actattccta aaacatagta
    11401 agaggttttt cctcctaaaa agcggatcaa acgattcgtt gtatctgttt cttttttcac
    11461 tctttttcct cctttatcta taaattcatt atagacaaaa gcgggtataa aaggaaaatg
    11521 atagaggttg attgcaaaaa tatcgtcaat cattcatata aactttctca tcaattaaaa
    11581 taagaaaacg cttgacagtt cttttaacca tatgataaat taaatcctgt tgctaaaacg
    11641 taactattac ggtttgcgaa aagcgaatcg gaacattcaa atgaaagaag atgagaagag
    11701 gagtgtcata aatgaaacga ttgataggta tattggccat gctggtttta gcaggtatgt
    11761 taactgcttg cggggcaagt gggaaagccg aggacagtaa agaaaaattg agcgtgatga
    11821 cgacctttta tcccatgtat gattttacaa aagcaatcgt cggggatgaa ggagaagtag
    11881 agttattgat tccagcaggg actgattccc acgattatga accttctgct aaagacatgg
    11941 caaagatcca agatacagat atctttgttt ataatgatga aaacatggaa acatgggtcc
    12001 ccgcaatcca aaaaacatta caagaaggca atgtccatac gataaaagcg acagaaggta
    12061 tgcttttact tccgggaagt gaagaaggac atgatcatga ccatgaacac ggggaagaag
    12121 gccatacaca tgagctagac ccacatgtat ggctagctcc aagtttagca atcaagcagg
    12181 tagcaaacat acgtgatcag ctgattgaag cttacccaga aaaacaggaa gtatggacaa
    12241 aaaatgcagc agcctatacc gagaaacttc aagcactcca tcagctttat caagaaacct
    12301 ttaagcaagc aaaacaaaga tcttttgtca cgcaacatgc tgcattcaac tatctagcgt
    12361 tagaatatgg attgaatcaa gtatcgattg ctggtctttc ttctagtgaa gaaccgtctg
    12421 cggcgagaat tgctgaactg aaacactttg ttaaagaaca tggaatcaac tatatttatt
    12481 ttgaagaaaa cgccaaagat agtattgcca gaacgttagc aaacgaagca ggtgtatctt
    12541 tagaagtatt gaatcctttg gaaggattga cgaatgaaca aatagaaaat ggagaaaact
    12601 atctttctat catggaagca aacctcgaag cgttgaaaaa aacgacagag acagaaaacc
    12661 cgctagaaga tactttgacc cccaaaaaag aaaagagcgt ctacaacggt tattttgaag
    12721 acgaggatgt acaagatcgc tctctttcag attgggcagg aacttggcaa acagtagata
    12781 cgtatgtaga agatgggact tttgatcctg tttttgaata taaagaaaaa ttgaatcagg
    12841 ataagacagc acaagaatat aaagaatatt atacgaaggg ctatcaaaca gatattgctt
    12901 caatcaagat cagtgagaaa gagatggcat ttacgtttaa gaatggaaca acgaaacgtt
    12961 caaattatcg ttatgtaggg aaagaaatct tgacctacga ggcaggaaac cgtggcgtaa
    13021 ggtatctatt cgaagcagaa gataaggaaa gtggcgcttt tagatatatt caattcagtg
    13081 atcacacgat tgcaccggct gactccgaac attttcattt atatgcagga aatgaaagtc
    13141 aagaagtatt gctgaaagaa atggagaatt ggccaacatt ttatccagat acaatggatg
    13201 gacgtgaaat tgcacaggaa atgcttttgc actgataggc aaaaaagaac cgaagggttt
    13261 ggatcggctg gtcatttagt cggtccaaac tattcggttc tttttatgaa atcaatttct
    13321 ttaatccacg gatagaggct gggatggtct cgccgtttgc caacgtgact tctctggttt
    13381 ttttattaat cgaccgcaca ttttgtgtat tgataacata ggatttatga caacggataa
    13441 aattccgatg cattttctcg atttctttga tcttctcata gaattgtaat tgggcatgag
    13501 tcaaatggac ttccagttta tgtggcgctc ctagcgaagt agaaaaatat tggatgtctt
    13561 tgatcggtaa aaatttgatc tcatcattaa actctatttt gaagatatct ttttggattt
    13621 gggacatttg aaaatcttcg aaaattttgc tcaggatatg ctcgacattt tcttttaaat
    13681 cataaagatc ttctttgacg atataatcag tcggttcgat gttgcttttt aagattttca
    13741 tcgacatttc cgtatgggaa gtcacataga ttatttttcc taaaggatct aattcacgga
    13801 tcaatttacc gatcgctaca ccatccattt cactgtcttc tagttcgata tctaaaaagt
    13861 aaataccaaa agaagaagtt ctgatcttgg ctgcattgag aagttcttcc ggatcagagg
    13921 tagaaatatg gacataactg tctaagtttt caatcatgat tctatttttg atcaccgttg
    13981 ataaaacttc taattgtttt ggatcgtctt cacaaatata aatgggaatc atatcattac
    14041 tcctttattt tatttgtata taaatcgttt gagaaaaacg ctggttttca attgatgttt
    14101 caaggaaaag ataaggattg gtctttgtca attcatccaa gataaacaag ccaaggcctt
    14161 cgttttttgg atttgttttt gttgagaacc ctttttgttt caactggttt aacggtaatg
    14221 tatttgttgt tgtattgatc acacgaatca tcacggaatg attcttcttg agtatggtca
    14281 attcgatttt tttcttcttt gcagcactag ctccttcaat cgcattgtcc aagataatac
    14341 caagcatacg tacaaggttt accgtatgtt tcgtatccag ttcgattggc tcaggaaaat
    14401 gaagctggac gatcaaccct ttttcctgtg ccatcatgag ttttaatgac agtaaactac
    14461 ggatctcagg aactttcata ttatccaaca tacttagatt ggccggaaaa aggttcatca
    14521 tctggcgtgt aggtaatatc gtttgatcat aataagaccg taatccttct aagtcgcctt
    14581 ctgaaatata gctgtgaaga gaaaaaagca gattttggta atcatgtttg aattctctca
    14641 agtcttgata gtttttttct agatctttga tataatcctg ttgcatcctg acttgttgct
    14701 ctaataactg atcagctaat ttcttattag agattttcca ataataaacc aagatgatca
    14761 ggccaacaca cagatataga atgaaaaaac tcgtattcaa agctaagaca tcaggtgttt
    14821 caccagaaaa acgggtataa aaaataatga tatagtatgt aaataatatc aatgtcccga
    14881 tcgtaccgat catacggcgg tccacagtgt gaaaccaagt ctctaacgat ttttttaaac
    14941 aaaaagctaa taaagcagat aaaaaagcgg ataagacaat gtgaaaatac tgcaatgaat
    15001 ctcctggttc aataaccgaa gatttcaaaa caaaaaaatc gccgatgctt gttaagtgat
    15061 ctcctaaaag cgacgtcaat aacccaaaag gaagcgcgta aaccaattgt tgacgcgaag
    15121 tcgaaaagaa gaaaaaagta agcatcagca ttcctatagc aattagagca gtaaaaatcc
    15181 ctaaaatcga atagacccat ccaatcaaaa tacaagaaat cagaatgact cccgcaaaca
    15241 gatatttgta tcggatgtga tggtcaatca ataagataca aattgtgtaa atcaaatatt
    15301 gcaacgctat ggaccataaa aacataaagt acagctccca ttgattttta ccaaattata
    15361 gcacaattgg cgtattattt ttttaatttt tcagggattt tgaattcaga actcatacca
    15421 aagcagcagc cagatacaaa aaagtcagat aagttagtta attgtttaat gattgttttt
    15481 ttcatgaaag attcctccaa aattcatcaa gtagtttgta ataggaagca acgtagctat
    15541 ttctaaaaca atcccaagta gcaaataggt agaaaagtaa gaagctgaga aaaataacgt
    15601 aatgagagaa aaaattagaa acatgctgat cgccttcttt aataatgggg gacgtttctc
    15661 gggttcaatg ggattgattg ctgtacctct tgatccatag cgaagcagta acaaaaagca
    15721 gctgattaac aaaaagacca tcattgggaa ggagatagtc aattgcagat gtttcatcac
    15781 caagggaacg ccccataaat aaagtagtgt ccacagcaga caaacgaatt cagagtcgga
    15841 atgcaatcca taagcatatc tgcggatgca gatataagaa agaaaggtga ttcctgtttc
    15901 cgcccatgag cctgtcacca gagaaaagac tagaacgacc gctagttttc caagattatt
    15961 caataaaact tctaagacaa attttacttg gacatacacc atatcttctt ccgatttgtc
    16021 tccgctgaat aaggcttcta ataatttgtt cacaacttta tctttcatct catacgtacc
    16081 tccaccacaa attttatgct aaaaaaaata aaaaacgtct tttgtttcat aaaaagtggt
    16141 tttgacatca aaaccgtcat gatctcctaa aaaaatcttc gttttccaag aaccttacca
    16201 aaaaaagtga cagttttgat acgaaaagta ctttttatga caagtttgtg ctttttcgag
    16261 tgatttatgt taaaaaatta ctgaccttat aaaaggtgat ggagggattc ttatggaaaa
    16321 tgaacaattg tttgaagtcg gcgaaacata tgcatttaaa tctgatttat ttgaatcacc
    16381 aataaaagga aaaatagaaa aaatctatga aaatagtgtg atgatacgtg tcatgaaatg
    16441 cgcagtagaa gacaatgata ccgctcataa tctgcactgg cagttagttg tacgcaaatc
    16501 aagtacaata gaagaataaa aacagataaa atgttgagag cataaagagg ttgtgagtct
    16561 cccgtttagc tctgagttac aaggaataat gttaaaaaac agcttttcat gttttatgaa
    16621 tattgccttg tgaccgaggt gttggcagga gaatactgtt tatttgaaaa aagggctgcg
    16681 acaagacttt tgtcacagcc tttattttca tgaataggag ttacttttgg ctacttttag
    16741 agaagtagct tttaaggagc agataaagtc aaggctttta tttctataaa tgaagtagta
    16801 aaatgaagaa cagattttat tacaggaagg atgccgataa ttgtgatcat tgttgcagga
    16861 acgatcggtg cagggaaaag cacactaact gaaatgcttg cccaagattt agaaacaaaa
    16921 cctttttatg agaacgtgga agataatgaa gtattgccgc tattttattc taatccagaa
    16981 aagtatactt ttttattaca gattttcttt ttgaacaaac gctttttggc aatcaaagat
    17041 gctttcagcc atgatgacaa cgtactagac cgctccatct acgaagatag tatgctgttt
    17101 catttgaatg cagatttagg gcgtgtttct gaagtagaag taaagcagta tgaaggtctt
    17161 ttagaaacga tgctgaagga gttagaagaa atttcgccac aaaagaaacc agatctactg
    17221 gtttatattc gtgtatcctt tgaaactatg ttagcgagaa tcaaaaaaag aggccgggaa
    17281 tatgaacagc tagaacaaga tcctgaactc tattcctact ataaagaact caatcgacga
    17341 tacgaagagt ggtatgaaca gtttgatata tgtcccaaaa tagtgatcga tggggacaaa
    17401 tatgatttcg tagccgatcc ggcatgtgga gaacaaatcg tacaagaaat caagatgcgg
    17461 gcaaagaaaa tgacagaaga ggcagatgcc cagtcgatcc gctacactac aaataacccg
    17521 aaagcaaagg gaatctttac aactaaataa tataaaaact aagcaaattg ctcactcgca
    17581 gatatttctc tgtgagcagc atcttgctta gtttttttga ttcattgaga tcaaatagtg
    17641 aaccgattcg tatgataaaa ttgccataaa tgaaatgtct gagtgagggc catgatcaaa
    17701 aataaaatgg aaacgatcaa aatcgaatgg gcgccaaaca gatgatgaca gaaaacaagg
    17761 acaatagctc ctgcgataaa ggcaagaacg acacttagat agtggaaaaa tcgatcaaaa
    17821 tctgcttttt ctttagaaat acaataatca tataagttcg ttgctacccc ttttaaattt
    17881 ccagttgtaa ataaattagt gtaggcacgc ccgttgattt tatcaaaagc tacccactgg
    17941 atcgatgcag taaaagcaat caaaatcgtt actaatgcgg gatgctcatg gacgggtgtc
    18001 aaactaacaa agaaaaaaat cactgcttca taatatagaa caaacaagcg ccagaaatgt
    18061 ttttcttttt ttctaaaata atcaattaga tactttgtta gaagaatccc taagaaaaag
    18121 aaaagggtag acaatacttt tttaccaaca cttagccatt ctccgtcaaa tgcttggatg
    18181 atcgatagaa tgatattgcc agtttgagca gaggcaaaag caccatattg gatataagta
    18241 taactgtcca tggctcctcc gataaatgtc agcaaaagtc ctattgtacg cccctcgtga
    18301 aaggaagtct gcattttttt catcaccttt tatgaatatt tttgtcttcc ttatcttagc
    18361 ataaacgatt gaaaaaaagg aaaaattttc agaaaactaa aatcatatcg cttcccattt
    18421 tcatttttag aaaggtctgt tagtataaag aaaaaataga caacgaaact ttaaataacc
    18481 aacttgaaat aaacaaattc aaagaagaaa gatgggaagg aaatcaatca gttgaatgaa
    18541 aaagaaattc tccgattaca aaaagaattg aaacgctggg agaacaagta taaaaagctt
    18601 caacaagtct ttcggctaca agaagaaatc attgctacct atgaagcggc ttatggcgaa
    18661 gcacccggac ttattgagga agaagtacaa gtaaaagaaa gtgaaggaaa cagctctttc
    18721 tttaaaatga tggagaaacg ttcctataat cgcttcaatc aagaacaaaa agcagctatc
    18781 acatatgata tgaagaaaca tcttcgaatc atagcaggag ctgggagcgg caaaacgcaa
    18841 acaatctgtg caaaagctgt ttatctgatg acgcaaaaac aagtagacga agaacgcata
    18901 ctaatgatca cttttacaag gaacgctgca aatgaactaa aaaaacgagt agataatttc
    18961 agccagagaa aaaccgctgt ccatatcgga acattccata gtatcttttt taggatatac
    19021 aatgaaattt gtcgaaaatt tcctgaagta gcaatgcaag gaatacaagg agacttttca
    19081 aaggatactg cacaaaaagt gaatgctgtt ttgcaacagc tgatacgtaa atataatctt
    19141 tatatatttg atcaatatgg agaaaaaacg attgcttctc ggttagacta ttggcagaat
    19201 atgaaccttt cagtagaaga aatggttcag ttagttaaag aaaaatatga ttccattgat
    19261 aagaatgcac gacagccgat atcagaacga ctgtatggat tgctcacgga actccaagaa
    19321 caaaaaagaa gccagcaatt gctagagttc aatgatgtac tccaaaatct gaaaagcgct
    19381 ttggagaatg aggagatcca gcattatatc ggccaaaaat atgattatct attcattgat
    19441 gaatttcaag atactaatcc acttcaatgg gaaatcgtga aacggatgac aaaggaaaac
    19501 aggatcaagt tgattgttgt tggagatgat gatcagagta tctatggatt cagaggatca
    19561 gagccagcat acatcaagaa ctttgaaaaa gaatatccta ccaaaactct tttcttatta
    19621 actaattatc gttctcgggc agctattgtc caaggagcaa atagattgat cagctataat
    19681 aaaaatgatc ggataccaaa ggcgatgatt ccagcacaaa aagaagcagg tgttatggaa
    19741 gcctatttgt tttctgatac taaagcagaa agtcagtggc taatcaaaca aattcagaca
    19801 tttattcaaa aaaatgggaa atataaggaa tcaatcatat tataccgatc agcttcacaa
    19861 acgcagcagc ttgtccaatc tctgctgaaa acgaatattc cttttgtact ggaagcagat
    19921 agtccatatg aaggtatctt tggtatcaag tctttccaac atttctatca aaaaattgtt
    19981 cattggcaaa cagcactgaa tctgcaaaag aaaaatcagg cctaccagca attgctccgg
    20041 cagctgatgg cagactgcta cctgaaaaaa agtgaatgcg atcaatactt ttctcctaaa
    20101 tatcaaaata tagccgttgc agattatatt ttgtcgataa gccccaattt aaaatcgaaa
    20161 tctgcagaaa tccgagagtt tgaggaagcg ttgcgtaaaa cagaccagca tcaagaaatc
    20221 tattcgcttg ttcaaagcta tcttcatctg ccacgtatag ctaaggaaat cgaccagagt
    20281 gatcaagaat ggatacgaga agaagtaaag cagcatgcta ctttcagaag actccaagcc
    20341 ttatcgcaag aaacccaagc gacagctaaa gaattaaaaa cccgcttgat tgcttacaga
    20401 caaggaaaac ttgatgcact atgtatccag agcattcaca aaagtaaagg tcttagttac
    20461 cgaaatgtct ttttgattgg ttgtaacgaa ggaagtttgc cttataacgg agcgacagaa
    20521 aagaaagtaa tcgactttaa tgaaataaaa gcagaaccag taacgacgat agaagaagaa
    20581 cgaagactat tttatgttgc gatgactaga gcaagaaatc gtctgtatct atgtgtacct
    20641 caaatgaaaa attcaagaaa attgaaagta tcgagattta tcaaagaaac cgggagtcgt
    20701 gtgaaaaaag gaaagtgata gaagagaggg tgtgacagaa gtggtcagtt tcaagaaaga
    20761 agaaggaatg tacgaaaatt gcttctcaaa tttttgtgaa atttcagctt attttcgaag
    20821 aagctatttc tggaacacag tttattcgga aaagggaggt gtgacaagaa ttttgtcaca
    20881 cctcccttat ttattttgaa aatga
//
LOCUS       DBKOIJ010000039        20729 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000039 DBKOIJ010000000
VERSION     DBKOIJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.039"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            31..900
                     /locus_tag="ACV5NJ_001079"
     CDS             31..900
                     /locus_tag="ACV5NJ_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HHY6485184.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            920..1267
                     /locus_tag="ACV5NJ_001080"
     CDS             920..1267
                     /locus_tag="ACV5NJ_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485185.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1280..2542
                     /locus_tag="ACV5NJ_001081"
     CDS             1280..2542
                     /locus_tag="ACV5NJ_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485186.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2622..3998
                     /locus_tag="ACV5NJ_001082"
     CDS             2622..3998
                     /locus_tag="ACV5NJ_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HHY6485187.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            3995..4624
                     /locus_tag="ACV5NJ_001083"
     CDS             3995..4624
                     /locus_tag="ACV5NJ_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485188.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            4636..5106
                     /locus_tag="ACV5NJ_001084"
     CDS             4636..5106
                     /locus_tag="ACV5NJ_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485189.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            5103..5585
                     /locus_tag="ACV5NJ_001085"
     CDS             5103..5585
                     /locus_tag="ACV5NJ_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485190.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            5582..6244
                     /locus_tag="ACV5NJ_001086"
     CDS             5582..6244
                     /locus_tag="ACV5NJ_001086"
                     /inference="COORDINATES: protein motif:HMM:NF019467.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HHY6485191.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            6231..6599
                     /locus_tag="ACV5NJ_001087"
     CDS             6231..6599
                     /locus_tag="ACV5NJ_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HHY6485192.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            6680..7126
                     /locus_tag="ACV5NJ_001088"
     CDS             6680..7126
                     /locus_tag="ACV5NJ_001088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485193.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRITEQAIEKQKDKEKKEIMSIYLSSGASFFHEID
                     SMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            7336..7857
                     /locus_tag="ACV5NJ_001089"
     CDS             7336..7857
                     /locus_tag="ACV5NJ_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485194.1"
                     /translation="MTINNNNPNKYNALVQCYIENKITMQEVADELGKSKSLVSHDFK
                     HNVFPNPIELRKKFQKRVYQEIGKWIDEGNAIDPLIEKYPFLKSKNKIQRLIRQGDIQ
                     AKEPVLTTNDLAKINLHKQIIEDFEKNLSTTEILKKYNIPRGKLTSTLFREYGESAVT
                     KAKEVANERKKQK"
     gene            7835..8434
                     /locus_tag="ACV5NJ_001090"
     CDS             7835..8434
                     /locus_tag="ACV5NJ_001090"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HHY6485195.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            8450..8926
                     /locus_tag="ACV5NJ_001091"
     CDS             8450..8926
                     /locus_tag="ACV5NJ_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485196.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            8943..9845
                     /locus_tag="ACV5NJ_001092"
     CDS             8943..9845
                     /locus_tag="ACV5NJ_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485197.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            9918..10109
                     /locus_tag="ACV5NJ_001093"
     CDS             9918..10109
                     /locus_tag="ACV5NJ_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485198.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            10273..10860
                     /locus_tag="ACV5NJ_001094"
     CDS             10273..10860
                     /locus_tag="ACV5NJ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485199.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            10860..11759
                     /locus_tag="ACV5NJ_001095"
     CDS             10860..11759
                     /locus_tag="ACV5NJ_001095"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HHY6485200.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            11759..12673
                     /locus_tag="ACV5NJ_001096"
     CDS             11759..12673
                     /locus_tag="ACV5NJ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485201.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            12725..12940
                     /locus_tag="ACV5NJ_001097"
     CDS             12725..12940
                     /locus_tag="ACV5NJ_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485202.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            13125..13379
                     /locus_tag="ACV5NJ_001098"
     CDS             13125..13379
                     /locus_tag="ACV5NJ_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485203.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            13558..14601
                     /locus_tag="ACV5NJ_001099"
     CDS             13558..14601
                     /locus_tag="ACV5NJ_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HHY6485204.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            14774..14998
                     /locus_tag="ACV5NJ_001100"
     CDS             14774..14998
                     /locus_tag="ACV5NJ_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485205.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            15001..15258
                     /locus_tag="ACV5NJ_001101"
     CDS             15001..15258
                     /locus_tag="ACV5NJ_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485206.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            15360..15713
                     /locus_tag="ACV5NJ_001102"
     CDS             15360..15713
                     /locus_tag="ACV5NJ_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485207.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            15766..16560
                     /locus_tag="ACV5NJ_001103"
     CDS             15766..16560
                     /locus_tag="ACV5NJ_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485208.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            16859..17707
                     /locus_tag="ACV5NJ_001104"
     CDS             16859..17707
                     /locus_tag="ACV5NJ_001104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485209.1"
                     /translation="MIEYDFNEAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKV
                     RPYENLRTYMFFLVNFEGTAKEGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLI
                     LDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQK
                     SLREGETSTFDKYSMYNRAYSSMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRS
                     EVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKGFENKGSFGKSKDEVKGVVIDDD
                     FEFDFD"
     gene            17740..18060
                     /locus_tag="ACV5NJ_001105"
     CDS             17740..18060
                     /locus_tag="ACV5NJ_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485210.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            18315..19004
                     /locus_tag="ACV5NJ_001106"
     CDS             18315..19004
                     /locus_tag="ACV5NJ_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HHY6485211.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            19041..19208
                     /locus_tag="ACV5NJ_001107"
     CDS             19041..19208
                     /locus_tag="ACV5NJ_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485212.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            19228..>20729
                     /locus_tag="ACV5NJ_001108"
     CDS             19228..>20729
                     /locus_tag="ACV5NJ_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485213.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDY"
BASE COUNT         7953 a         2667 c         4288 g         5821 t
ORIGIN      
        1 ttatgataac acaataaaag gaatgatggt atgaaaaaga agaagtttag tcgatcgttg
       61 ttaggaattt tagttttagt tgcggttggt cttagtggca agggttactt tcagaataac
      121 agcactcagg aacatgattt tcagatcaaa agtagtgttc aggtaggtaa taaagcagac
      181 agtgaatcaa aagaattttt agggatacgt tatgatccaa acaaatatcc aaataatatc
      241 gtaaacatta atgatgggaa atatacactg acaaaagaag accatgaagc attatcagaa
      301 aaaggcagtc aaaccttttg ggtaaattat gcagatttag atcaacttgg aagagcagga
      361 gatgttgaag cgttgatcac ctatgatgct ataaattctc actcatctaa aaatgtggaa
      421 cgtccatctt tcaaaagttc tgtccatatt gcaggagaat acaaggatgg agtatttgat
      481 caagagagag atacttgggt cggcaaagat tcaaacaatg aaatcactca gctagaagga
      541 tataaaggtt atctctataa caaaagtcat tcattagcat ggtcgctagg aggagatatg
      601 gaggtacaca atttaacact tggaacgaga gcgcaaaatg ttgggaaaaa tgatcagaat
      661 ggagggatgg cttactcaga aacgattgta cgtgatgcaa tctataaaaa taagaatgaa
      721 aaagtattgt ataaagttga tccaatttat aaagggaatg aattggttcc tcgtggttct
      781 catgtcgaag ctttctcagt taacgataat ggaaaaacgg taaaccttaa tgtttatgta
      841 tttaacaatc aaaaaggaat caacatcaac tatcaaaatg gtacttgggt aagagattag
      901 aaattgagaa ggtgaagaat tggaaacaat aagcatcaat ggattagata aatcagttgc
      961 aaatcgaata aaaaaaggaa atggctacta tcttctgaag aagggtaaat attcagttgc
     1021 tagagagtta gaaacaatga gtatcgtgta tgtagctaag aatcattttg acgtagaaca
     1081 ggctattttt gatccagatt ctgaaatagg gaaatgtgtt gaattatcag agaaaactgt
     1141 tagtacaaaa ggggcgatga tcattgagag agaaatgtta gacgaaatca gagtaatcat
     1201 tcctaaagat acgcagatgt gtgaggacag cataagtgac gatgaaccaa aatcagaaag
     1261 aaaataagga gatattcaga tgagcgaaaa atccattgaa ttaaaggaat ataagcgaag
     1321 cgatggagaa aaatattatc ctgtagccag tttttctaaa tcagtatatc tggttggacg
     1381 tgcattggta gttggtgcaa ttggcatata tattttgcat tgggtaacaa aggttgtaca
     1441 actgcttgta gataccttag taagtattct tgtctcgtta aatgatgtta acggaacaga
     1501 tgatttagga ttaaaagaaa tgtttaaaga gttactcaat gcgttttttc ccaattattt
     1561 aagttattca tataatgtac aaagcttttt tgcaaagcta cacttggtag acatctctat
     1621 tttagtatta gaagcaggaa cggtattctt tgttggatat ttggttactt tgatgttcag
     1681 gcatcactat ggagaacaaa gaccattttt tgatgatatg gaaagtagaa ggttgaaaag
     1741 agaagctcta gaagctatgg atgtgaagta tgatccaaac aagattatag gagatgaaac
     1801 gaacaacaaa aaaagaaaac gaaacaaaac gcaattagaa tccgatgtgc tatcaacgct
     1861 taaaaatatc aaaatttctg ttcatacaag acgtgatcta agtgtcaatg aagatattcg
     1921 agaatatatc gtaaaaatcc cgcaaccaaa agggaagaaa gttagagaaa cagttttacg
     1981 acaaattcaa gatttagggt cgatcctgac aagagcaaca agcggacgag ccaaatttgg
     2041 tgaaaaagaa gatgaagttt caggcgatta tttcattttc cgagcatcag taaatattga
     2101 aagagaacgt aaggaaagag taagtaccaa aaacaaagaa acggaaagag caacatcttc
     2161 aatcgcagtt tcaaattcac aattttcttt tccaatcaat ctctttgccg ataattccaa
     2221 aaatatccaa aaccaaaccg aaaaagctag aaaatatgcc gaaactcaaa agcaatcttt
     2281 agaactttat ctatcttctg ctgacgtacc tgcaagtcta acagatatgc acgttgggaa
     2341 tacatctatt gaatatttgt ttactttatc tcgtaccaga aaaattcaac taagctcaat
     2401 gaaagaagaa attgaaactt acttgcatat atcaggtgta atcgtgcgga tcaacgcagg
     2461 gcgtattcaa attactctac cattaggaga aaatgatcgt attccaattg atgtaccatc
     2521 aatgatcaat agcgtgtttt aaaagacatc acaaatagaa acagaaaata caaactacca
     2581 gttcacaaac agaaaagtac cgagaaaagt gaggaataga aatggctatg aaattattag
     2641 agaaagtcaa aaagagagcc gaaaaagctg aatctatttt gctagataaa gaacctaata
     2701 taaagcttaa tggaaaattc agtgtcaata tgttgaacac aaaggctaaa gacccgacag
     2761 atgctatttt tggaattaag cctgatggca caattgctca tttttgcatc agcgaaatgc
     2821 cacacatgtt ggttgcagga acgacaggat cagggaaatc agtattactg aatgaaattc
     2881 ttgttacagc aatgtgtcac tcaacgcctg atgaattaaa aataggaatt gtagacccaa
     2941 aaagggttga atttggaaga tacaaaaaat tgccgttcat gttggctgat ccaattacag
     3001 atatggatga agcttatgat ttttttgaat atcttgtgat cttgatgcat gaacgttaca
     3061 aactgatgga aaaagcaggg ttacaaaaca tcacgctata taacgaatat gccgaaaaaa
     3121 atggattaga tagatttccc tatgtgatct tattagttga tgaatattca cagttggttg
     3181 gaacacataa agaagtagaa ggtttgattg ttcaattagg acaaatggca agggcagcag
     3241 gtattcatgt tatcttagca acacaatctc ctcgatcaac agttgtcact ggtattatca
     3301 aagccaattt tccgtcacgt gtttgcctta tggtagcaag tgatttagaa agtaggattg
     3361 tattagatga aggcggtgga gaaagtctaa gtccaaaggg agatatgctt attaagcttg
     3421 ttgatggaag tatggttaga gcgcagggag cttacatcag taacaaagaa attgaaacaa
     3481 tcttcaatca ccttagaaat actatgccag aaccagaata tgttgattac cgagcaatcg
     3541 tagctgaaac tcgtggtgag ggcggtgaag acgaggattt tggtggaatt ggtaaagctc
     3601 ctgtaagaaa atctgctaaa agaaatttga acacaccttt agcaaatcca tcatctaaag
     3661 agccatcaat tgaaaagaag agtgcgtttg ctattaagct tgaaaaaaat aaagaagaaa
     3721 aagctaatca gggagctaag tcactagaaa aacttcagca agaaaaatta gaaaaactag
     3781 aaaaagcatt atcatcatca acttctgaaa ctcaatcttc aaagggtata gataatggac
     3841 aatcaattac aactaaaaac aagcctaaaa atagtaattc aaatctctcg gtaaatgttg
     3901 ttatcacaac tgataaatct gaaaaaaaac aagtgaacgg aactacttca cccatcatgc
     3961 aaaaaatgat aaaggcaaaa aaacaggtgc agaaatgatt atcattagtt cctttttgag
     4021 atggctaaca ccatataaat atacagatga aatagttgag aagcgagcga agaaaaaagt
     4081 taaaaaaacg atgttaactc ctaaagaaat gctagatcgt ggaatattgg gagtgaaggt
     4141 tttagaagta aaccaaccgt ttgaattgga taatgcgaga gataggttgc ttagagctga
     4201 agttgatgtc gcatactttt atcatgataa aagcaagcat caaatagcta tcaaattcaa
     4261 tccaaaatcg gcatggcgat accaagcaag agatgaccta aagcgtagga agctgaaacc
     4321 gatgctctat cctgaaggaa aagaggcatt tgatagaacc catattgttc ccattggatt
     4381 tcatggtaca gagtcagata accgcctatt ggtagggtgg aacagctcac aaaatagagt
     4441 tcagatcaat aaatttgaga agaaggtaag aaaatttaac gatcggaaaa taatactttg
     4501 gtttgccaat attcagaagc aaccagatgg gacagcaaag tgggaagcgg tcatatttga
     4561 tgaagatggc aaaaaagtaa tgtctgaagt atggcatgat aaagctaaat ttgtttggca
     4621 gtagaggtga aaattgtgtt aactaaccta aaactatata gagagggatt taacgaaaaa
     4681 gtaagtatcg gtgaatctat tgaatattta ggaaagaagt acattattat tcgtattctt
     4741 gaaattaaaa aactttattt tagtaaatat aataatttag cagttagagt tctcgtccaa
     4801 aaggttggaa atccaagtga ctatagaagt gattaccata actataaaac agaatcatct
     4861 gtaattaagc gttataatca atcaaaaaat gatgagagaa tattcaaagt aggagaaatt
     4921 attatttcta agtatgggat agcatacgaa atagtttcta ttaaaaaatt ttattatgaa
     4981 tttgttgatt tggttgtaga atattctgtt cgattgatcg ttccgtggag taaagaagaa
     5041 attaatgagg cattaaaaca tgaacgaaaa agtacattca aagttttaga aggtgaaaaa
     5101 aagtgaaaac agaccttact aggcaagctg agaagtgctt atggcatgaa acaaatcgca
     5161 tgggtgtatt tggttgtttc gaagtcacta tcggttggga tggcagtgaa agagtagatt
     5221 atataactta ttcagtagat aacacagttc gatgttatga gatcaaagtt actttagctg
     5281 atttaaaaag caaggcgaaa cagactttta tcggtgatta taactattta gttgttacta
     5341 gtgatttgtg ggagaaaatg caacaagacg ataaacttat gcggaaatat tactatcaag
     5401 gcgtatacgt gttcaatgaa aaatatccta gcataataag tgttaagaaa gcaaggaaac
     5461 aaaaagtatc gctaggtaca agatcaactg tattagaaag catggttcgt tctatgaatc
     5521 gtgaagttaa gaaactctat aaagtaaaac cattttggga gatagcagag gtggaaaaag
     5581 gatgattcaa acatttgcaa aaatgagtag aagtgcttgg attgaaaagc tggaaaatga
     5641 tttgagtgtt atgcgattag ctccaaagaa attcaaacga gagattgcag aaaaagaagc
     5701 aattttgtcg gtattgaatg cggtagaact ggacgaaccg aaaaagtcag ttgatgtacc
     5761 tgaaaatgta gcagagttac tagattatta tcgtaattcg tcagatgtcg atttgttagc
     5821 tctgatatta acatttcacg attggtatta ctgtcaaaat gaagatactc agcatgataa
     5881 acatgataaa gcagttgact ggttaataga gaatccagaa aagttcatgc gtgcttggct
     5941 agatggctat gatagcaaga aaaagccact ggaaagtaat gaggaaacgt atttaacggc
     6001 agaacaatat gactatgcta gaaaaacagg agattgggaa tctggtaaga ctttcaatca
     6061 cagaaaagat caaccaaaaa ttagtttttc agatatagcc gcaattatcg aaggtatcta
     6121 ctatggacaa gccgatgaag agatcttttc tatacaagat ttattagaat ttttgaacaa
     6181 aaataaggat agctttgtag aactaactga ttatgaggag gatacagcaa atggaaaatt
     6241 atgatccaaa tatgcgatgg ggtacacata cgcttaaagt ttcttttcaa cgttgggatt
     6301 acaagggatt tgtgactttt agaaaagctg gaaattgtaa aggattagat gtcttagcgt
     6361 tggatgaaga ctatctatat gaccatccat tgacggataa tccaatcgga tttggattgc
     6421 ttcctgaaga tgatgagggc aatgaatggt tcaaaatgat tcttacaaac gataaaggtg
     6481 atcaattatt tgtagaagat acttggtcat atttgagtga atatattgtt agcattaaaa
     6541 tcattgattt tgtagcagat aaagaaaaag agattggaga aggtaaatct aattactagc
     6601 taatgaatag caactttgga ggataagtca aaagaataaa aaaagaatac tgtgtgtcat
     6661 tgcagggatg tatctttgca tgatagtatc tgcggttacc atttattggt taggaaatca
     6721 gcaaaacagt ttaattgttg atctggggga gttaaaggtt ggattacgaa tgactagatg
     6781 gcttttatta gctcaaacag ttggtttagc catattgact tggatatgtg tggaaaaaat
     6841 aacatctaat aataatcaag agattgttga atcaacaaac ctaagaataa ctgaacaagc
     6901 aattgaaaag caaaaagaca aagaaaaaaa ggaaataatg tcaatttatt tatctagtgg
     6961 agcatcattt tttcatgaaa tagattcgat gaaactgagt agtcaagaaa tcattgatag
     7021 atggaaaaac atgcctctag gtggtgagtt aatcgttgaa gatagtgatg gttggtcagc
     7081 ttataagaag gacgagattg tagctatcac gattttgttt gaatagtaat atattaccac
     7141 tagataatct gaatatgttg atctgctagt gacgtaccaa atgcgtttaa tagaaccttg
     7201 gtcacaggca gaaattgata aagcagtaaa attgaaccga ttatcaaaat ttaacgtatt
     7261 aggaaatgcc ttttaaaaat cgcaatacac aaacagaaaa ataccaaaaa attagcaatg
     7321 aacgtaggta agaacatgac aataaataat aataacccta acaaatataa tgctcttgtc
     7381 caatgctata tcgaaaacaa aattacaatg caagaagtcg ctgatgaatt aggcaaatca
     7441 aaaagcctag tatcacacga ctttaagcac aatgtttttc cgaacccgat tgagctgagg
     7501 aaaaaatttc aaaagagagt ttatcaagaa atagggaagt ggattgatga agggaatgca
     7561 attgaccctt taattgaaaa atatcctttt ttaaaaagca aaaataaaat tcaaaggtta
     7621 attcgtcaag gcgatattca agctaaagaa cctgttttga caaccaatga tttggctaaa
     7681 ataaatcttc ataaacagat tattgaagac tttgaaaaaa atctttcaac aactgaaatt
     7741 ttaaaaaaat acaatattcc gagaggaaag ctaacatcga ctctttttag agaatatgga
     7801 gaatcggcag ttactaaagc aaaggaagta gcgaatgaga gaaaaaaaca aaaatagtag
     7861 tcgatactta gtagcgactg tagtttttgt ttgtgtattg attttcctgt ttgatttttt
     7921 acagattgga aaaagtataa ttggaaatac attgagtgca aaggaaatgg ttgctttagg
     7981 tggtttgtat gaaggacatt tgttcccgaa tatattcatt tctatcgttt ctcatattaa
     8041 cttgtaccat ttaatttcta atttattgtt cttagtagtt cttgaagaag cactgaatga
     8101 tcagtatgag tggttaacat tattgatcgt tttaatcata tcaggatttc taggaagctt
     8161 agttcctttt ctgctacaaa gcaacatcgt aagtatgggg cttagtggag gtatttttgg
     8221 atggattgct ttatttgcga ttgcatacat ccattttgat agcaaggaac atttattact
     8281 aagtgttttt gtattattga taatcggcat tttattggta tctacttttg aatcaggcga
     8341 ggcaaatgtc ttgtcacatg taatcggatt tgtctgtgga atgatcttgg gatttattct
     8401 aaagaacgtt tcattgatca aacaaaagaa ttaaaaggga gtgctaaaaa tgccaaaaag
     8461 aaaagaaaag aaaattccag tgtttttgtc tgtctcagaa atgttatcac ttcaaatact
     8521 gttaagaaaa aagattgaaa caatagacga agaagttaga aataaaaggg ctatgtacga
     8581 acaacgtgaa gcgctaagag aacgaatcca ctattttgat gatatacaca ctaaaatcta
     8641 tgaagcacgt acaaatcacg aagacgaaag taaattagga ttagctcaac aacggatact
     8701 tgaatggctt aatacgaata ctgatcaaga accgttaaga gtgattagta atctttttga
     8761 atttggaacg aagcattggt ttaatgctgt agagaaggat gctgacttac aagaagcgtt
     8821 tgatacgcta agtaaggata gcaaatccat gcgagattta ttgattactt atctaaatga
     8881 gcatgagttt gaacaaacga aaaccaaaaa gaaaaataaa gaatagaaag ttgtgatgaa
     8941 tcatgctatt aaccctgtta ggttatttat gtttaattct tgcttttttt ggggcaatcg
     9001 ctataattat tggtcaattt caaacaaata atccgattat gcgtaacata ggcattggaa
     9061 ttgtgattgt aacctttatt gtgtttaatg ccactaagat ttttggaagt aacgataaac
     9121 aatttggtat atcgggagtt aataatagtt atgctccaga ttctttggga agggtagtaa
     9181 tagatggaac atttgaaaat gtaacgagtt ataaaaatgt ttcgctgaca gtgaaggaag
     9241 atggcaagaa agcatcagac gacgttctcg ctactattaa gccgtctcaa tcacaatttg
     9301 aagtgtttta tacagttaaa gagaatagcc ctgtacacaa gctgacattg gtttttaacg
     9361 ttgatggaga agaatactat aaagatgtaa aaataaataa aacatcaacg aaaaagatta
     9421 tcgaatcagc gaaaaaagaa gtagctacag aagaaagtac aactgaatca agttctactg
     9481 tcgaagcgga actaccagat aaaattgatt ttgatcctga aaaagtcaaa tcgtttgaat
     9541 ctgtttctgg ttctatggct gatcggttta acactgaagt cttcaaagct atcaaaactg
     9601 ttaaattgga tactgaaaag caaatgatta atgttacctt attcaattca gttgaatcta
     9661 tggatcagga taaaaaagtt acggttgcaa gagaactagg aaaaagagta attggtttgt
     9721 atcgtggtac tgctaaacca gattcagacg gtgcagattt gccagttcat gtagtttatg
     9781 aagacggtaa agtttttgga gattctacag caaacgaagc attaacgatt aaattggcta
     9841 aatagccaga aagttataat aaacaaatgg tgtcacacca ataatatata ggtgtgacac
     9901 cttggagatg atagctgatg gtagtaaata aagaaaaagt tagaaagtat gtgactttaa
     9961 aaatagcttc tgttaaatat ttagaaaagc gagctaaaga agaagaaaga gcaagaggta
    10021 agaaagtaac agtttcagag ttgttagaag aattgatcga gaatgatcaa acaataagac
    10081 gtgcatttac taataggtct atcagataac aatataatta ataaaaaaga aagagtgatt
    10141 tggtcttaga ctaaatcact ctttttatat aaagtttctt tacactcatt ccgaataatg
    10201 ttaaaattct ttatatgata tatagttcct tgtcagagga agagataacc agcaatattg
    10261 gagggataat aagtggaaaa caataatgat attagacatt ttagcattgg ggaaagagtt
    10321 acaacagtaa gtgaattatc aaaggattat agagataaag agggaacgat tatttctctt
    10381 aataagaatc gaagaggcgt ttggcaatat gttgttgatt taggtgatgg cgttacagtt
    10441 gagtgtaaag atagtcaact gatcagaaat aatggcagtc attccattga aaaagcaatt
    10501 cttgtatacc caaaagtatc aagtaagatc atgaatgact ggttgcttgg aaaatttggt
    10561 gagtacaata aaaaagaaat cgcagaagtg atcggcaaat cttatccaac agtattaggg
    10621 tatctaaaaa agataacgag taaagaagca aaaattgagg atgttttaac agtaagagag
    10681 cttcaatcat taatcgagta tcggaataaa caaataacaa atcctgagta ccttagaaaa
    10741 gaagatggtc tattgaaaca attggaacaa gtaaataaaa tccggaagat gcaaaaggta
    10801 gctcctttaa gccttgatac ctttaaaaaa atctatttga aagagatcaa aaaagactaa
    10861 tgtggactgt aactttaccg ttccaacctg tatctgcacc aagaccaagt gcaagaagga
    10921 atgctgctga tgaaactaat gacgtagcag agaaaaggat ttcaacctac tatgataaaa
    10981 agtatcttga ttatttgaaa aaaataaaga gctttatcga agatcaagat ttattagatg
    11041 atgaatttta ctcgattgta aatgatccta tgggcgcaat tatggaagtg gacttttact
    11101 accagatacc aaaaagctat aaaaaagtct ataacattat gaagacaaca gcgccagatt
    11161 tagataattt agtaaaaagt gctatggatg gtatttttaa catctcagct atacgagata
    11221 gccatatcac aggcttaatt gcttttaaat ataatgtggc gaacgaagga aaaacagttg
    11281 tgaggatcaa acgatattca gaatttgagt gggatacttc tgaaggattg aacggagata
    11341 tatgggaagc tacatttgat tttaaaccag tagcaacgcc aagaccaaag tctaaattta
    11401 gaggaaatgg gattatcact tactatccaa aagagtacaa cgactatttg gaaaatatca
    11461 agaatacgct taaagagaaa gatttggtaa atgatcaact gaaaaaggta atgaatgctc
    11521 ctatgggggt tatagctcag atagattatt tttaccaagt gagaaaagat aagaaaaagc
    11581 ttgattcact gatgcgtact tcacagccgg atattgataa tttagtcaaa ggaacattgg
    11641 acggaatttt taacaaggaa ataggcatca aagatagcag ggttgtaggg ttaattgctt
    11701 ttaaatacaa tacttttgaa aagtcgaaaa caaacgtgag actacaggag atgggctgat
    11761 gaataataat gaaaaaattc ttcatgtact tgatagcttt gaaataattc aagaagaact
    11821 aaaaaaatat cgggatgttt tggaacaaag atatgatttt gtcaatcagc aaaagagtaa
    11881 tcacatggat ttcattctca atatgaatga cttgaagaag aaattagttg aacgaaaaga
    11941 acaagaaaag ttgatcaaag cttactttga actcggtgaa aaagaagtta aaaatgcaat
    12001 ggaactgaat gaagagcgca gggttttaga tcagctgtta gaacaattat tggtgatgtt
    12061 tcaaaaagga cgaatagatg aggatttaat tgaagaagga ttgagaaaat atccttcaaa
    12121 ctcaggtatc ggaatagtgc tgaaagcgat tgatgaagaa gaaattgaag ctttcatacc
    12181 gccagaggat tttgaatcgg caatggaata tataaaatat tatagccaag ggatcactgc
    12241 ttttagagag tttgatcctg aagatgttat tcatgacttg aacaacctaa aagaatggtg
    12301 tgaaagttat ggagtcgatg attccggatt ggactacttg atcagtatta tggaaatcga
    12361 agaagagatg ccggataagc ctgatccaac agatatttta gaattgatcc atgaagctag
    12421 aaatccaatt gcctatattt ctagaggata tactgtattg gaatactata agccttatat
    12481 atcagcgatg aaccatttaa gacgtgtatt aagggaaaaa cgtgaatacc gctcagtgtt
    12541 gaatgcaacg aatagattag aaaaagctgt ttcagagcta gatgcttatt atcgtgaaca
    12601 ttacctgcaa gcaggtggta tgccaagaaa tacgaaagca aatatcagta ggtatataaa
    12661 aaaagctgag taggcggagt tagcccataa aatggcaaaa ggtaacctga aaggggcatt
    12721 ttgaatgaaa agggatgttt ttgaattgta cgtattagaa cgtctagaag acggcatgat
    12781 ggcacaagag ataattgatg aaattgatga tatgatcagc agatttggtc aattggagtt
    12841 gaatgatgga aacaaaaatt cggtagtgct aaaacaattt tggttgaaca atttttcgta
    12901 taaggatttt atcaaagaaa aggcaaatac attgtactga ctaaattcac gaggagcagt
    12961 gatgagagct tattgctaag agcagttagg ctcttttttg tttgtgtaaa attatttgtt
    13021 ttataataca catttataca caaaaaatat tgacagatag tacacaaatg tggtaggata
    13081 tatttgtaag caagttagat taacaagtta aaggaagagc agttatgaaa aaagaagaac
    13141 taatagatat atttcaaatt gttgaacgag caaataatat gggaattatg ttttttgatc
    13201 gtatttcttt aaagatggat ttaagcgttg cccaccaaga atttaatctg cgattaaaag
    13261 ccttgttgaa ttctgatgat gttaatttcg ctcatgatgt tgtaggtata cagaaccata
    13321 tagatagaga aaacaaacgt atgggtgatg aattcttacc aagatattca agtttatgat
    13381 ttggcgaaag attcaagagt atgtgtcatt attgataggt actcttgaat tggaaaggag
    13441 gaatgaattt aagacaacaa cgattatgat cttaaaacaa tcgtttatgt aattggcaat
    13501 cttacgaata attttcatgc aataatgaca ataaaaaatg aaaaggggta aaatcaaatg
    13561 aaaaaatttt tagtaggaat tctattcaca atggggctag tattattatc accttcagtt
    13621 tcagcagaaa gctctatttc catgtatcgt atgtataatc ccaatagcgg agagcatcta
    13681 tacactagaa gcgttggaga aagagacaat ctgaaacgtt atggatggca ttacgaaggt
    13741 gtcgcatggc aagctccaac aagtggtact cctgtttatc gtttgtataa tcggtataac
    13801 ggtgaacatt tttacacagt tgatgccgga gaaagagata agatttcaca cacaggatgg
    13861 acgtacgaag gtgttgcgtt ctattcctac aacggagcaa atagagttcc tgtaacacgt
    13921 ttatataaca aatacgtgaa ttggcatcac tacacgttag atcaaaatga aaagaacgta
    13981 attagtaaac aaggttggaa gattgaaggt acaggctggt atgcaatgcc aaacagcgta
    14041 ccaaatccta cacctgctcc aagcactgaa aaagtgttga acgttcctgt tgtatggcaa
    14101 ggaaatacaa tgttatgcga aggagcttct ttattagaag gtttacacta caaaggtgta
    14161 gccacaaatc aaagcttaac cagttttgta aactatatgc caaaagctaa tgacaataat
    14221 ccttatcatg gttactcagg agagtggcgt cataacgtta atggaacgta tcaaggcatg
    14281 atggctgacc cagtagttaa atgggcaaaa aacgttggcg gtaatgccgt caatatcact
    14341 ggttcaggaa cgaatggaat caagaatgaa attcgcaatg gtaatccggt tgtaacttgg
    14401 attacttata actatgctac gccagagttc aaacaaatgc cttggggtcg tgcagtatgg
    14461 aatggacatg tagttctcgt ggatggcttc agaaatggtg cataccatgt tgttgaccct
    14521 gtatttggcg caaaatggat caatgcaagc acgtatgaga gagcatttaa tgtgactgga
    14581 atggcggttg ctgtacgtta attgatagaa ttatcaacta aatgaaataa gagcttaatt
    14641 actgaaatgg taaataggct cttatttttt tagaggaaat ttttgtaata cgaatctata
    14701 acaaaaaata ttgaatgtag tacacaaaag tggtatatta aaatcgtaga gattatgcga
    14761 agaggtgatt ttgatgaacc aaagaagaag aaaaaggtta gaagaaataa gcgagcgact
    14821 ttccgagcta aaggatgagt tagaagtaat tcaagaagaa gagtacaaag aaaatatacc
    14881 taaaaatcta cagggatcag aaagatatga aaaagcagaa gaatccgttg atcaacttga
    14941 agaagctgtt gatggtttat ccgacatatt agaattgatt gaagatatag ctagctagag
    15001 atgattaatg aagatttcag gagtgagaat aaaatgattg atatttcact gatagagaag
    15061 ttacttttct caaataagta caccgcaaaa gagctggcta caagatggca aatatccgaa
    15121 tcagctatta aagcctatcg tcaaaataag ggaagcgtag gatggcgtga ttggaaaggg
    15181 atcagcattg gaaaagcaga agacataatg aaacgtgaag gacttcgatc ctataccact
    15241 tctgaaaaag acaaataact taatgtttga aaagataaaa tatctgtctt aaagctatgt
    15301 caaattcaaa tggtgaatgt ttgctaaata taatgcaaaa aaaggaaggg aaatattaaa
    15361 tgataagcga aaatttgtct tatgagaata ataaacatct agttaaagaa aataagaaag
    15421 tagaagatta taagaacatg tatatagaaa tgaaaaaatc accactgtgt attcaatata
    15481 aaaatgataa ttggaagcaa gtagtcgaat ttgtaaaacc tcatagtata cgaagagtac
    15541 gaagggggaa gaaaaaaagt gaacttgtaa atagcggaag agcggtaatt agagttataa
    15601 ccaaagatta tccagttgaa tatggagatt atattttaag gttaagttat catgcttttg
    15661 ttgtgttgaa agaagagcat ttcaacgagt ttttcaattt aaaaagtaag taatcatatt
    15721 tttttgtaga cgtgtataaa gttaactaaa ttgcgaggtg atgacatggt agcaaaaaag
    15781 aatactctaa aagaagcttc tgaaaaatat caagatatga aaagacgagg attaataccg
    15841 aaagacaaaa aaggtatatg gagagaattt gttaaggaag tcagtagtga tcgaagtggc
    15901 ggtgactttg ttggacgata tacagcaaaa ggctatagca aacgattaag gatcactgtt
    15961 gaatatggaa cgaatgaatc agctacagag ctgaagcaaa ggtacttaga tacacgaagg
    16021 gaagcttcaa aaaaatataa tgatttcaag agcagaggtc tgattcctaa aaagaaaaag
    16081 ggaatttggc gtgagttttt caaggaagaa gatagcaatc ggaataaggg agacttcatt
    16141 ggacgataca cagcaaaagg ctacactaag aatttaggga tcaaggttga atacggaact
    16201 ggtgaaagtt catgggaatt gagaaatcgc tatttaaata cattgttaga gaaatataaa
    16261 gcagaattat tggagttgtt cgatgttaat gattttatcg agatggctga aaagtatggt
    16321 catgaagaag gttttatgat caatgacatt atggttattg ataattttac ggatttatta
    16381 gcacttgcga aaaaagcaag ggatttaaga gaaacgcaga tgtatctatt cagaaataaa
    16441 aacggaagaa accggagaac tgaatcgagt gagattgaag attactacga tgcctttaca
    16501 cacgatctgg gagcctttgt agacgagata agtggtgtaa tgaacaaata tagggtctag
    16561 aataaaaaag ccggagatga gcagataaat gggaaacaat agcatataca cacctgaaat
    16621 gcagaataaa attaaaagac ttgtcggatt agggtggatg acaagaggaa aattagattc
    16681 ttttagtacg atcaaatatc ttttgatgga catttaccgt cagaacaagc aaacaggaat
    16741 acattttttg ggctatgata agccggaaga tgcacctttc tttgttccaa gttcagccta
    16801 tagagccagt acctccggta gcatgtataa cttggatttg tttgaggaag gagcaaagat
    16861 gatcgaatat gatttcaacg aagcctattc gaggatcatg agaaattatc ctttgccgtc
    16921 aaacacttat ttgaaagcaa aatataatac agatcaagct attttgagag cgataaatag
    16981 accaaaagta agaccgtatg aaaatttaag aacgtatatg ttctttttgg ttaattttga
    17041 aggaacagcc aaagaaggaa catatgctaa tccaaaatca cagtcgatgt ttgcggatta
    17101 tggcgaaaat cccaaaacag ctaatgagcc tatttggtta tcggagattg agctaacact
    17161 tatccttgat tattatgata ttaaagagat ggaagtccta gatagccaca catttttgac
    17221 gaaaaaagga ttactcaaag aatattttga aagaatcgag ccattaaaaa ctgatcctga
    17281 tacgttgaag ctttataaaa atcttagaac gaaagtattt ggtgctatcg gacaaaaatc
    17341 attaagagaa ggagaaacaa gcaccttcga taaatacagt atgtataatc gagcgtattc
    17401 ctccatggta gcaggcgtat ttagagatcg tatggttaga tatgaacaga aatacgttca
    17461 tagcgcatat gggctattaa aaattcgtac tgatggattg tatttcagat ccgaagtacc
    17521 agagtttgaa gagctgtata aacttggagt agttaagaaa aaagttcata caatatccaa
    17581 agaagaagtc gaaaaagctt tagaatttaa aaacaaagga tttgaaaata agggctcctt
    17641 tgggaaatct aaagatgaag ttaagggtgt tgtgatagac gatgatttcg agtttgattt
    17701 tgactaaata ctctttggaa aagatggtag gaggtaaata tgaagaagaa aattgtggaa
    17761 ggaaatgtag gtgttattat tctaaaagat tcaactgact atgctaaatc aatggcacta
    17821 aaagacgaaa aagttgcacg catggataaa gatttagttg aatttctgat ggatgataca
    17881 gaaagattaa aaggggatca gtttttgagt ttaatgaaag acaaaggctt aaacacatat
    17941 ggtttatttg attataagaa attagctata gagtgggtaa aagaaggaga aatatttttt
    18001 attgaagaat gggaaggtac tgagaactta gttcttcatc agtatctttt gaaagcttaa
    18061 tctaagtaaa acagttctga ctagttatgc ttgtcagaat ttcaaaggaa gttttccata
    18121 accatcatca ttcaaaaata aaggatttga aaataagggt tcctttggga aatctaaaga
    18181 tgaagttaag ggtgttgtga tagacgatga tttcaagttt gattttgact agattttttt
    18241 gaaaggttta agagtaaaca aggtaagaac taagatcatg aagattgaga gtaaagaaga
    18301 tagaggacga atgtatgaga acacactata tttatttaga agataaaatt gtagatgaaa
    18361 ttccggcaaa aagtcgagaa caagctagaa aattatttat tgaaaaatac aaagagatca
    18421 atggagatag tgaagttcca aaagtggtcg taacagttag aaaaaggcaa acaaaaaaga
    18481 gaagtacgtt tcatgtcagc cctgatatat ataatcttat agagcgacat agaagtgaag
    18541 ataatatttc tatctctcaa tatattgcta cagttattga tgaattgatt tcaggaaagt
    18601 atgactatgc ttttgatttt gagggtgaaa aggattacaa aagtgttgct gtctttcaat
    18661 ctgattttga gaagctaaaa aactatgctg gcaggagaaa tgttaatttt gcaagactac
    18721 tagaagagtt ctactccgaa gagttgggag taggaaatga atacaagaga gaaacttatg
    18781 cgaaattgtc tcataagtta tcccaagcgc ttaaagaagt gacagaagtt aaagcggaac
    18841 tagatgctaa acttgaagaa gtgaaaaaat atcaaacaga gaggaagaac aacatgaaga
    18901 atcaagtgga atttgacgaa cagaaagaat ttgaaaaagc aagaaaacgc tataacaaaa
    18961 ttatgaatga acttagctat gaacacttaa cagttcatga ataattgagt gagggtacag
    19021 aaaaatatac gatcgcagat atggttaatg aagtggaaac tcttttagct tctttctttg
    19081 aaaatggtca cgtaaacgat gaatggcgaa aagaagaacc taaaagggtt aacgcttata
    19141 ttgctcgatt aaaaaggttt attgataaat ataaggagaa tagtgacaaa ttttctaaag
    19201 ataaataagg agagagtgtg attacatttg ccagaaatcg aaggaatatt ttcgccaaaa
    19261 ataggtaaaa aacagttaga tttcagcagt tttccgagcg taaatagaga ttataatcta
    19321 ggtgatgagg tacagatact aaatattgat ttcaaagaac tagacttcgc tgttgctgtt
    19381 tccaacaaac ccaaggcatt ggaacgagtg aaaaaaatat accaagagaa tgatcaaaat
    19441 atcaaaaata tgatcaaaaa gtatagacga gataattcaa aagttgaaga cgttttattc
    19501 aacggtacgg caaaacatga tttgaatgtt tatatggcat taggagtggc tttgtactgt
    19561 aattcaatta atcagacaga tattttggtt aatatcgtga ggaatagcta tcaaaaaata
    19621 ttaaaaataa gtagcgaaac aaacccttat tatgcaatga aatatcttgg ggataattta
    19681 ttaaatgata aaaaattgat gcacaacact gaagagctgt tatctaattt ggctgcgatt
    19741 aactttctat tcaaaacagg ttctgaagat tattttatag ataacgtact aaaaggtgct
    19801 tacggaacag cattttatcc gctttacaat atgaaacagc ataaagccga acaaaaggat
    19861 aacaaacttg aaattaaaaa gatgaaagaa ctattaggta taggatttgt aaaacccaat
    19921 tcaaaagtca ctattgcttc gttagcaact actaacaatg atgggaaaat agtaaatggg
    19981 tttgacgatt cagatataga tatattgtca aaatcatatt tttctttgta tggagatttt
    20041 gcagatttga tgggtattga tagttactta gtcaataatt ttgagatcaa gggtagcgag
    20101 ctgaatgata ttgtgtattt cgttttaatc tctctatatg caattgatca gaataccgag
    20161 taccgacaaa ttgactattt gctagttttt tatagtttgt tatatcagtt cgttttaact
    20221 cggttgttta aaaatgctaa ggaagaattg atcctgaatt ctgattatga aatagcaaaa
    20281 caaattgcaa gtattagaaa agaaaacaaa tctgaagtaa tcaaattaag aaaagaatta
    20341 agtattgcga gtaaagaaaa cggtcggtta gaatctttag tcaaatcggc aaaaaaagaa
    20401 gaggaacttt tgaagcaaga gattaaagaa ttaaaaggaa tcagaaaaga acttgaacag
    20461 ctgaggtttg aaaataaagt attaaagcaa ttgcaggagc aagaacatag acaatcatta
    20521 aatggtaaaa ctgaagagcc ttcactgtct gaaatgatag cttatctaaa acataaaaac
    20581 attcttttag tgggaggaca tgtaaatttt caaaataaat taactgaact attaccaaat
    20641 attaaaatgt ttgatgtaag cgaaaataat ggaaagatac ttaaaaattt gaatacgtgt
    20701 gatgcgattt ttctatatac agattactt
//
LOCUS       DBKOIJ010000040        20387 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000040 DBKOIJ010000000
VERSION     DBKOIJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.040"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            23..247
                     /gene="nrdH"
                     /locus_tag="ACV5NJ_001109"
     CDS             23..247
                     /gene="nrdH"
                     /locus_tag="ACV5NJ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HHY6485214.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            406..2565
                     /gene="nrdE"
                     /locus_tag="ACV5NJ_001110"
     CDS             406..2565
                     /gene="nrdE"
                     /locus_tag="ACV5NJ_001110"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HHY6485215.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2615..3580
                     /gene="nrdF"
                     /locus_tag="ACV5NJ_001111"
     CDS             2615..3580
                     /gene="nrdF"
                     /locus_tag="ACV5NJ_001111"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HHY6485216.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3669..4808)
                     /locus_tag="ACV5NJ_001112"
     CDS             complement(3669..4808)
                     /locus_tag="ACV5NJ_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHY6485217.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5117..5830)
                     /locus_tag="ACV5NJ_001113"
     CDS             complement(5117..5830)
                     /locus_tag="ACV5NJ_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HHY6485218.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6084..6785)
                     /gene="nagB"
                     /locus_tag="ACV5NJ_001114"
     CDS             complement(6084..6785)
                     /gene="nagB"
                     /locus_tag="ACV5NJ_001114"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HHY6485219.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7019..8551
                     /locus_tag="ACV5NJ_001115"
     CDS             7019..8551
                     /locus_tag="ACV5NJ_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HHY6485220.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8742..9944)
                     /locus_tag="ACV5NJ_001116"
     CDS             complement(8742..9944)
                     /locus_tag="ACV5NJ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6485221.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10026..10715)
                     /locus_tag="ACV5NJ_001117"
     CDS             complement(10026..10715)
                     /locus_tag="ACV5NJ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HHY6485222.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10821..11189)
                     /gene="rplL"
                     /locus_tag="ACV5NJ_001118"
     CDS             complement(10821..11189)
                     /gene="rplL"
                     /locus_tag="ACV5NJ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HHY6485223.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11242..11745)
                     /gene="rplJ"
                     /locus_tag="ACV5NJ_001119"
     CDS             complement(11242..11745)
                     /gene="rplJ"
                     /locus_tag="ACV5NJ_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HHY6485224.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(11949..12076)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12155..12844)
                     /gene="rplA"
                     /locus_tag="ACV5NJ_001120"
     CDS             complement(12155..12844)
                     /gene="rplA"
                     /locus_tag="ACV5NJ_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HHY6485225.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12948..13370)
                     /gene="rplK"
                     /locus_tag="ACV5NJ_001121"
     CDS             complement(12948..13370)
                     /gene="rplK"
                     /locus_tag="ACV5NJ_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HHY6485226.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13713..14381
                     /gene="sdaAB"
                     /locus_tag="ACV5NJ_001122"
     CDS             13713..14381
                     /gene="sdaAB"
                     /locus_tag="ACV5NJ_001122"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HHY6485227.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14394..15266
                     /gene="sdaAA"
                     /locus_tag="ACV5NJ_001123"
     CDS             14394..15266
                     /gene="sdaAA"
                     /locus_tag="ACV5NJ_001123"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HHY6485228.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15357..16457)
                     /locus_tag="ACV5NJ_001124"
                     /pseudo
     CDS             complement(15357..16457)
                     /locus_tag="ACV5NJ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(16549..17436)
                     /locus_tag="ACV5NJ_001125"
     CDS             complement(16549..17436)
                     /locus_tag="ACV5NJ_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHY6485229.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17513..18058)
                     /gene="nusG"
                     /locus_tag="ACV5NJ_001126"
     CDS             complement(17513..18058)
                     /gene="nusG"
                     /locus_tag="ACV5NJ_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HHY6485230.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18159..18329)
                     /gene="secE"
                     /locus_tag="ACV5NJ_001127"
     CDS             complement(18159..18329)
                     /gene="secE"
                     /locus_tag="ACV5NJ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HHY6485231.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18353..18505)
                     /gene="rpmG"
                     /locus_tag="ACV5NJ_001128"
     CDS             complement(18353..18505)
                     /gene="rpmG"
                     /locus_tag="ACV5NJ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HHY6485232.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18667..19311
                     /gene="cbpA"
                     /locus_tag="ACV5NJ_001129"
     CDS             18667..19311
                     /gene="cbpA"
                     /locus_tag="ACV5NJ_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HHY6485233.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19367..19870)
                     /locus_tag="ACV5NJ_001130"
     CDS             complement(19367..19870)
                     /locus_tag="ACV5NJ_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HHY6485234.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19883..19929)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         6317 a         4119 c         3668 g         6283 t
ORIGIN      
        1 tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca
       61 atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat
      121 cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt
      181 cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc
      241 cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc
      301 accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt
      361 tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat
      421 ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa
      481 atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca
      541 aatacgatga cttttctatc tattatggaa aaaatcaatt acttgatcga tcaagaatat
      601 atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt
      661 ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa
      721 tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc
      781 ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa
      841 atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt
      901 cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg
      961 cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta
     1021 tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc
     1081 gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt
     1141 caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca
     1201 gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc
     1261 gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca
     1321 tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat
     1381 gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa
     1441 aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca
     1501 gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt
     1561 ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaacg
     1621 gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt
     1681 ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc
     1741 gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc
     1801 atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta
     1861 gactttacaa atatttactt catgctgttg aattattgga cacttgttga aagcaacaat
     1921 atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc
     1981 acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca
     2041 ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta
     2101 atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca
     2161 tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa
     2221 gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg
     2281 tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaatggca
     2341 caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa
     2401 ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga
     2461 cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat
     2521 gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt
     2581 cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat
     2641 aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca
     2701 attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc
     2761 tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt
     2821 ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc
     2881 cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt
     2941 tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc
     3001 ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga
     3061 aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc
     3121 tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat
     3181 ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga
     3241 gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct
     3301 ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga
     3361 agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc
     3421 actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg
     3481 cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga
     3541 agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa
     3601 atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt
     3661 ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca
     3721 tgttccattc ttctcaataa atagattttc cggatattta atattatcgt cttaatcact
     3781 gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc
     3841 aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg
     3901 atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga
     3961 aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca
     4021 tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg
     4081 aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg
     4141 ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga
     4201 atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata
     4261 aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg
     4321 atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat
     4381 aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc
     4441 aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga
     4501 attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt
     4561 acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa
     4621 acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaact
     4681 gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg
     4741 ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt
     4801 gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt
     4861 tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag
     4921 tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc
     4981 ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga
     5041 aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt
     5101 tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt
     5161 gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat
     5221 agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga
     5281 ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga
     5341 taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga
     5401 gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg
     5461 cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt
     5521 gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa
     5581 tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc
     5641 ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa
     5701 ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg
     5761 ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca
     5821 tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa
     5881 aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt
     5941 tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat
     6001 atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa
     6061 cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca
     6121 acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca
     6181 atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta
     6241 cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta
     6301 tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt
     6361 ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag
     6421 atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc
     6481 agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat
     6541 tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca
     6601 tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat
     6661 aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct
     6721 tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt
     6781 tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt
     6841 tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga
     6901 atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag
     6961 caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat
     7021 gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg
     7081 tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg
     7141 taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca
     7201 ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt
     7261 aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat
     7321 tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg
     7381 gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga
     7441 gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag
     7501 tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct
     7561 gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt
     7621 tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca
     7681 gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct
     7741 aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc
     7801 tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg
     7861 atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc
     7921 agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc
     7981 agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt
     8041 gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac
     8101 tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga
     8161 taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc
     8221 actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact
     8281 agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg
     8341 ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct
     8401 cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca
     8461 gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt
     8521 gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga
     8581 gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga
     8641 ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct
     8701 tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga
     8761 tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc
     8821 ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa
     8881 ccagccgcac ctccaataaa gtacataaac ataaatacag tattatttcg gttgcttgct
     8941 gcttcaccta gcccctgtac tcgtgtttga ttggcgactt gaccaaattg attcccgacg
     9001 tctaataaaa taatagctaa aagcaaaatg gccaaatggc ttccgccaac caataaagca
     9061 ataaagctta ttgtttgcat aacaagtccc atatacacga tttttctttc agaataagaa
     9121 tctgccagtc gtcccacgat aggtgcagca actgctccag acaaaccaaa aatcgctaat
     9181 atccctgctt cgaatgttcc ccaattgtag gcgggactgc tgatataaaa aatcaacgta
     9241 gaccaaaaga tcgaaaatgt tccaaacatg aaaaatccac tgactgccgc ttctcttaat
     9301 agacgttgag aagcaactaa atgcggcaaa cttttaagtg aatcaaggta tgaaacagct
     9361 gtactacttt gctggacaga acgcggcatt ttccagtaaa gcagaagtgt aagaattccg
     9421 actgcacata aagcaaataa atagatcatc cgccaagaaa aaagactggc aacaatacca
     9481 gataccgtcc gagaaagcaa aatccccacg agtaaaccgc taagcaatgt acccatgacc
     9541 ttccctcttg cttttggtcc cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg
     9601 atcgaaagta atcctattaa aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa
     9661 aaagcggcca acagagagac ggcagataac gctgccattc gaatgatcaa ttttggacga
     9721 tccaccacgt cccctaaagg aactaaaaac agtaacccta acgcatagcc taattgggtc
     9781 aacatcgtca atatcccgac tgcacttgtg ctaacagata aactagctgc tacttttgtt
     9841 ccaatcggct ggatgtaata catattggca acaacaacgc cacaagtgat tgataatagt
     9901 aaaataagcg aagttgttaa aggttttttt tcattactct tcattgattt cccccatttc
     9961 ctatcttagt ttacaactta tggaaaagat tggaaataga aaaatacaac tattcgccac
    10021 acttattatt tgtgcttttg ttttccttgt tttttcttgc gatctttata aatgaagaca
    10081 actaacccta ctagcacagt aacacttgca accaacgtga ctgcagtttt gttatatccg
    10141 tgaaacagag cttctccacc acacgcatac aacacagacg aagggatgac tccaatcacg
    10201 atcgacaata tgatttgctg tagaggcaat ttcatcgcat ctgcagcaaa attgaccata
    10261 gaagatggaa taaccggtat catatagcct aacattacac ctatttttgg atgtttcatt
    10321 tgtcggattg cttgcaccca atgattcgtt tcggttttct tatctaaaaa ttttaagcga
    10381 tgcataagga aaattgcagt gatgttacca agagcattac ccaaaatgtt gatgatcgca
    10441 cctgtcagtg gtccaaagct caaaccagca atgactccaa ctactgaagt aggaatacct
    10501 ggaacagcac acatcaccgc tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc
    10561 gaacgaatat gtgcgagtag ctctgcctta ctcgctctcg gatttaaaaa taattggatg
    10621 tctgtctgat attctaaata caaccgatag cccactacca gtatcagaat gattcccaac
    10681 gagatcaata tcaatcggat tattttcttt ttcatatttc ctcctgaaaa aaatctatat
    10741 tcattaaata atagtttaac ataaaaacaa aaaagcaccg aaatccaaca taggatctca
    10801 gtgcttttaa atagtaaaaa ttatttaaca gttacagatg cgccaacttc ttcaagttta
    10861 gctttcaatt cttcagcttc ttctttagat acgccttctt taactggtgc aggagcgcca
    10921 tcaactacag ctttagcttc tttcaagcct aaaccagttg cttcacgaac tgctttgata
    10981 actttaactt tttggtctcc agctgcagtt aattctacag tgaattctgt ttgttcttca
    11041 gcagcagcag cgccaccagc agcagctaca gctacaggag cagcagcaga tacgccaaat
    11101 tcttcttcaa tagctttaac taggtcgtta agttctagga tagttgcttc ttttaactca
    11161 gcaacgatgt tttcaatgtt caatgccatt ataatttcct ccgtttaatt gttctttttt
    11221 atttaatgct ttatgcggca attaagctgc atctccatct tctttgtctg ccactgcttt
    11281 gacagcgtaa gccacgttgc ggactggcgc ttgtagtaca gatagcaaca tagatagaag
    11341 tccttcgcga tttggcaatt ttgccaatgc tgtgatttct tccaatgaag agactttacc
    11401 ttcgatgata ccgcctttga tttctaatgc ttttgcttct ttagcaaatt cgtccatgat
    11461 tttcgctggt gcgactacat cttcgttact gaatgcaaca gctgtaggtc cagtgaatac
    11521 ttcgtccatg ccttcaaggc cagctttttt agctgcacgt gaaagaatag aatttttgat
    11581 aactttcatt tctacgttag catcacgaag ttgtttacgt aaacgtgtta cttcatcaac
    11641 tgttaatccg cggtagtcaa cgactactac tgatgcagct gctgtaaatt tttcgcttac
    11701 ttcatcaacg attgctgctt tttttgcgat aattgcttca ctcatttttg agtttcacct
    11761 cctgaatttt gatggaagag ttttttgcac tgaaaaggca agtcactttt atagttactt
    11821 tgctcaacca gtattattca tcacctattc acttcgttca tagtgattct actcgctttg
    11881 ctcgaaagga aactcctttc gctttgctca accagtattg ttcatcatct cttcacttcg
    11941 ttcatagtga ttcacaataa aaaactctat gccaccgcag acatagaggg actattgaat
    12001 atattcaata aacgtcctcg gtaggaaatt aaggcttgat gccacctact gtcttcggta
    12061 caagttaatt attaacctca actagcttag catgctaaac tagttgagtc aagaattatt
    12121 tttcagttgg atcaatcaac attctaatat ttaattagaa agaagcttgg tcaacatgga
    12181 taccaggtcc aaatgttgtt gtgatcgtta catttttcat gtattgacct ttagctgctg
    12241 atggtttagc ttttagcaat acatcatgga ttgctgcaaa gttttcaact aatttttcat
    12301 cgtcaaatga aacttttccg attggtacat ggatatttcc agctttgtcc acacggtaag
    12361 ttactttacc agctttcact tcgtttactg cttttgttac gtccattgta actgtaccag
    12421 ttttagggtt tggcattaag ccttttggtc ctaatactcg tcctagacga cctacagtag
    12481 ccatcatatc aggtgttgca acaactacat caaagtcaaa ccatccgttt tggatttttt
    12541 gaaccatgtc gtcgtcacct acgaaatctg caccagctgc ttcagcttct tttgctttgt
    12601 ctcctttagc aaaaactaaa acagtttgtg ttttaccagt tccgtttggt aatacaacag
    12661 caccacggat ttgttggtct gcttttttag ggtctacgtt caaacggtat gcaacttcaa
    12721 ctgttgcatc aaattttgca atgtttgttt cttttgctaa agctactgct tcggcaacag
    12781 gataaacttt acttgaatca acttttttta atgcatcttg catttttttg ctctttttag
    12841 ccattttggt tcctccttga ttgtggttat aacggttgta cctcccacgt gttctatatc
    12901 tttcaatata gagccaactg agaagctgct gtcttagggt gtatagatta ttctacagtg
    12961 atccccatgc ttcgtgcagt tccttcaacc atgcgcattg ctgcttcaac gttagctgcg
    13021 tttaggtctt ccatttttaa ttcagcgatt tctcttactt gatcgctaga aactttcgca
    13081 actttatttt tgtttggttc gcctgaacct ttttcgattt tagctgcttt tttcaataga
    13141 acagcagctg gcggtgtttt tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata
    13201 acaaccggaa tgatcaaacc tgcttgatca gctgtacgag cgttgaattc ttttgtgaat
    13261 cccatgatat tgatacccgc ttgacctagt gcaggaccta ctggtggagc tggagttgct
    13321 ttacctgcag gaatttgcaa tttaacgatt ttttctactt tctttgccac gagacatacc
    13381 tccttaagtc cgtgatgtgg ttaattggag atgcatattt ctcctcccac gttttatctg
    13441 atctgaaaaa atccagacaa ataatgtgca aaacaatgca cactggtaca gtatagcaaa
    13501 tttatttgtt ttttcaagat ttttttctgc tatttttaca ttttgcttga ataaacgcta
    13561 aatttattct aaaaaaacaa caatttttcg ttctcatttc ttcttttatg ttacacttgc
    13621 gctagcatgg ttcaacagat gaaagcaaac aataacacgg ttttttagac ccttgtacaa
    13681 agattcctac ataacaaaat gaggtggaga aaatgaaaga tttaaggtat agaagtgtct
    13741 ttgatattat tggacctgtg atgattggtc caagtagctc tcacactgcc ggagctgcta
    13801 gaatcgggaa aatcatacgt agtatatttg gcgaacagcc tgattctgta gatatttatc
    13861 tatatgaatc atttgcaaaa acctatcggg ggcacggaac agatattgca ttagttggcg
    13921 gcctgttagg gatggaaccc gatgatgaac gtttagctga ttctttgaaa ttggcatatg
    13981 aagccggtat ggaagtcttg tttgttccta agagtgaaaa agccgaacat ccaaactctg
    14041 taaagatgct tctaaaaaaa ggaacacgaa agctttcagt cactggcatt tcgatcggcg
    14101 gtggcaatat ccagatttct gaattgaatg gattcaaaat ttctttaagc atgggtactc
    14161 ctaccttgat cgttgtccac caagatgttc caggtatgat tgctaaagtg actagtatac
    14221 tttctgagac caatacgaat atcggcacta tgacagtgac acgggaatca aaaggtgaaa
    14281 atgccattat gataattgaa gtagatgatc ctcaagtaga agaaacggta aaaagactca
    14341 aacagctgcc aaatatcgac agcgtaaatt atttcgatta aggagctaaa aaaatgtttt
    14401 tatcgattga agaattagta gatcaagcaa aagcttatcc aagtgtagca gaattgatga
    14461 ttgcagttga aatggaaatg agtggccgtt cccgtagtca catcatcgag atcatggaaa
    14521 aaaacctctc agtaatggaa caatcaatca acgagggaaa agaaggtgtt acctccgtca
    14581 caggaattac cggtggtgat gcaaaaaaat tagatgatta tttaaatgag gcagactttt
    14641 taagtggaga aacaatctta aaagctgtac gcaatgctgt agctgttaat gaagtcaatg
    14701 caaaaatggg gttgatttgt gctacaccga ctgccggaag cgcgggtgtt gttcctggtg
    14761 tgctgatggc tgtccgagat cggcttgatt tgacacatga acagcaattg gaattcttat
    14821 ttaccgctgg cgctttcggg ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag
    14881 ggggatgtca agcagaagtt ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg
    14941 caaaaaatgg gactcctcat caagcagcac aagcagtagc cattacattg aaaaatatga
    15001 tgggactgat ttgcgatccc gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg
    15061 ctttaggctc ttctcaagct tttatctctg ctgatatggc attagcgggt atcgaaagtg
    15121 tcattccgcc ggatgaagtc gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga
    15181 ttttcaaaga aacagcagaa ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg
    15241 aagagatttt aaataagagg gcgtgataaa gctccttaat ttcgagtatt aagagattag
    15301 ttatcttcat aacaaaaaac gaagagagga ctaattgccc ttctcttcgt tttttattaa
    15361 tccatcaatt tcgtatattt tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg
    15421 atcgacaatt ctttgttcag gtgttcaaaa acagatgtta ctccttctgc cccacctaga
    15481 ttcaacccat aaatcactgg tcggccaatc gcaactaagt cagcaccact tgccagagct
    15541 ttgaagacat gctctcctcg acggacacca gagtcgaaaa tcactggtac acgtttgttg
    15601 actacttcgg caatttttgg caatacttca aaggacgcag gacctccatc tagttgacgt
    15661 ccaccatgat tggataccca gataccatct gcccctgcag agatagcgat tactgcatct
    15721 tctggcgatt gtattccttt tacgataact ggaagatggg tgatttcttt gattgttttg
    15781 atgtcatctg gtgtcaagcc ttgttttgca gctgcataaa tttctgcgat tccttttcct
    15841 tcgccattac cacttgcact ttgttcgcta tacgcagcta agttaggcat tggtaatgga
    15901 aattggaatt gattgatgac gtcttcttca cggtaaccac cgagcgttga atctgccgtt
    15961 aaaatgatag ctttagcccc tgcttctact gctttgttca aaatgaactc attgaatctg
    16021 tcgtctttac tcatatataa ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg
    16081 tctttgattg tcgtatttgc ataagtgctg atggaaaaga ttgatccagc agcagctact
    16141 cctttagctg tatcagcttc ccctttttca tgtgccaatc cttgggccgc tgatggcgct
    16201 tggataatcg gtgtatctaa ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg
    16261 attccgcgta atatccgtgg catgattttc tttgtattaa aagaagtcgt attttctttc
    16321 atcgtccact catcttcaga accaccgcga atatagccaa aagctcctgc ttccatgcgc
    16381 tcttttacac gaccttctag ttaggcaatg tttacaatat cgattggatg ttcttcatta
    16441 cttgtatgat aagtcatagt agatccttct ttcatcttct tgattacata taaatcatac
    16501 taaacttaca aaaagtaagc aaacatttac tctcagatac ttttatcgct attctttcca
    16561 aataaatcga cttacaagct gatctacttc ttggttttca tgaagcatac tatgagaggc
    16621 attttcacct gtcactactt tttcctctat ttcatttccg cgttgcttca acaacgcata
    16681 gaccgctaat gcactgttta acggtactgt tccatcactc aagtctgttt catctagttg
    16741 accagcaatc aagaaaaatc gagtggttat aggaatatta ccgatcattt gctggtaatc
    16801 ttgatagcgg ttagaaacga cagcaggccc ctttgctaac tcatctgtca gagactgcgc
    16861 actatcatcg acaaaatcat taaaaggcgc gccaattgct gttaatgtct gaacttgtgg
    16921 caatgacgta tcctgtccat acgttcctaa atagcgtagt gcactaactc ctcccatgga
    16981 atgccccact atgttgactt tatctatgcc gtagttttct ttcagataca aaagacaagt
    17041 tttgatccat tctgcttgat tccactcgtt atttttatta tcttcaaaca atacttggac
    17101 catcggattg tctgcttttc cagacaaatc tcctgttgct tgtatttgtc catctgttcc
    17161 tactgttaaa accaattctt ttactgcttg ctgatttttt tccaaacgcc gcaacatccc
    17221 tttaaaggaa ttttctgtcc cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt
    17281 ttctatcact ttactggatt ctgccgtttt ttctttatca gaaacttcag tagtatttat
    17341 cttcgtcgtc tggcaaccac ttaacgcaaa caaactgata aggactaaag ctgctgcagt
    17401 tgcacgtttg cataaatttt tgattggttc tgtcatacac ctcttccttt ccatatagaa
    17461 aaaagaaaga cttgaaaggc caatcatatg atcgacattt caagtctctt tattaatcga
    17521 ttttatctac ttgatcgaaa tcaagttctg tactggtttc acgtccaaac atatcgatgt
    17581 tgactttcaa tttttctttt tctccgtcaa cttctgttac ttgtccttct aatccagcaa
    17641 atgcgccttc gatgattttc acgacttcgc caacttctac ttctagatca ttttgtcgca
    17701 ggctcatacc gatagaacgc aagatatgat tgacttcttc tggaagtaat ggtgctggtt
    17761 tgcttccggc tccgtgtgaa ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat
    17821 accatgaagc gtctgtcatg atcatttcaa caagtacgta tcctgggaat gttttatgaa
    17881 cgatttcttt ttctttccca tttttgactt cagtttcttt ttcttcagga acaacgacac
    17941 ggaaaataaa gtcggccatt ttcatacttt gcgcacgaga ttcgatattt gcttttactt
    18001 tgttttcata tcctgaatat gtatgcaaga cataccagtt tttttcaaaa gattccattg
    18061 aaggctcctc atttcattgt aaaataaaaa aacctcagta cacccgaagt ttttctttcg
    18121 cttcattata gcactaaaag tacgttgtgg ctagcgagtt attttaaaat ccagccaaat
    18181 aagctttgga tggctgtatc cattacgtaa aacatcaatc cgaaaataat agaagtttcg
    18241 atgactacta acgtatcttt acggagttgt tttctgcttg gccaagttac ttgtttgatt
    18301 tcatctttta cgctttttag aaatttcatg agacaacctc ccttgctact gtttatttcg
    18361 tttctttatg taatgtatat tgattacaat atttacaaaa cttattgatt tccaaacgtt
    18421 cgccgctttt tccttcactg actgattttg tgtagttgcg agacccacaa acggaacaag
    18481 cgagtgctgc ttttttcttt gccatttact tctgccccct tgctgagaat cagacttata
    18541 cttcataaca gtatcacgta actttttact tgtcaatttg tgaaataaac tatctattgt
    18601 tttggataaa attatgatat gattaatttg aaattaagct aaaatgagac aattggaggt
    18661 atagcgatgc tattaaaaac acttgtatat aagaaaaaag atctgacaac cgttacagag
    18721 actagtacat tagaagaagc attaaaaatt ttagaaactt ccggataccg ttgtgtgccg
    18781 atcttagatt cttcaggtaa tattttccgt ggaaacattt acaaaatgca tatctatcgt
    18841 cataaagcaa atggcggtga tatgacgctt cctgtcactc atcttttgaa aaacgctaca
    18901 aagttcattt acctggatac ttctttcttc aaagtattct ttacgatcaa agagcttcct
    18961 tatattgctg tccttgacag tgaaaatcat ttttatggta ttttgacaca tagtacgttg
    19021 cttaatatgc tctctcaatc ttggagcgta gaacaaggaa gctatgtact gacaattgct
    19081 tccacaggaa agcaaggaga tttggctgct attgcaaaaa tcatttcaaa acacagcagc
    19141 attgccagct gtattactct tgatatcgaa aaagacgaat acattcgacg tacattgatc
    19201 acgctaccgg ctaacactga agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa
    19261 aagaattata aagtggtaga aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc
    19321 gaaaaaagaa aacagctaat ctgctttctt ttttcgcttt ttataattag acacgctttt
    19381 caaaatcaag cgcgcgatct aaataataca tcaatggtgc tgaaacagcc atgacgataa
    19441 actcacttaa tgcaagcgtg ccataggttg tccaaaatgc ttggccacct gttggaacta
    19501 acatataagc aatcagtccc atgctgatgg taaagaagat cgtgttcaat actaaacgca
    19561 tctttacatt tttcactttg ttttgcaata aagctgtcag gcctaaagaa agcaatgttt
    19621 ggccgccacc aaaaaccaca tccatgatac caaaaccaaa gaaggcatta tagacgatga
    19681 ccccgcctaa aactccccaa agcaatttac ggttaaagac aactaaatga ttcaaacttt
    19741 ctgaaatacg aaactggatc ggccctgagg atacaggcgc aactaaaata gttaacgcta
    19801 gataaagagc catgatgatg ccatttacaa cgattacgga taaccgttgt ctgcttttcg
    19861 ttttttgcat tttctctcct cttttctcct agttttttta acgcgggatg gttgatgaac
    19921 cgcggttatg tgaaacaaac aataattata acagactttt catttatttc aatccgtctt
    19981 atctgctttt aaaaaagcaa aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat
    20041 atggtataaa gtaagtgtaa ggggtcataa gaattcccag taaagcagac gaagtaggat
    20101 ctgcaagaac agaattgttg tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc
    20161 aacctcttac tagagaacgg tctagcaagc agctgaagat ctaagtggac atgccgaacc
    20221 ttggaacttc tccttacgta agtaatggat catgcgcttg ctaggctttt ttgtgttttt
    20281 cggtcttcgg acctttttca ttttcctaga cttctgctat aataaccaag aattaatttt
    20341 gtaacagatt tttttgaaaa aacaagtttc tttcattcaa aaaatag
//
LOCUS       DBKOIJ010000041        20325 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000041 DBKOIJ010000000
VERSION     DBKOIJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.041"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(273..346)
                     /locus_tag="ACV5NJ_001131"
     tRNA            complement(273..346)
                     /locus_tag="ACV5NJ_001131"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(310..312),aa:Ile,seq:cat)
     gene            complement(535..1554)
                     /locus_tag="ACV5NJ_001132"
     CDS             complement(535..1554)
                     /locus_tag="ACV5NJ_001132"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HHY6485235.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1551..1775)
                     /locus_tag="ACV5NJ_001133"
     CDS             complement(1551..1775)
                     /locus_tag="ACV5NJ_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HHY6485236.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1772..2065)
                     /locus_tag="ACV5NJ_001134"
     CDS             complement(1772..2065)
                     /locus_tag="ACV5NJ_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485237.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(2102..2239)
                     /locus_tag="ACV5NJ_001135"
     CDS             complement(2102..2239)
                     /locus_tag="ACV5NJ_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HHY6485238.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(2241..2672)
                     /locus_tag="ACV5NJ_001136"
     CDS             complement(2241..2672)
                     /locus_tag="ACV5NJ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485239.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2686..2979)
                     /locus_tag="ACV5NJ_001137"
                     /pseudo
     CDS             complement(2686..2979)
                     /locus_tag="ACV5NJ_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3180..3629)
                     /locus_tag="ACV5NJ_001138"
     CDS             complement(3180..3629)
                     /locus_tag="ACV5NJ_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485240.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(3633..4250)
                     /locus_tag="ACV5NJ_001139"
     CDS             complement(3633..4250)
                     /locus_tag="ACV5NJ_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485241.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(4250..5158)
                     /locus_tag="ACV5NJ_001140"
     CDS             complement(4250..5158)
                     /locus_tag="ACV5NJ_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HHY6485242.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(5171..7930)
                     /locus_tag="ACV5NJ_001141"
     CDS             complement(5171..7930)
                     /locus_tag="ACV5NJ_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HHY6485243.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(7927..8631)
                     /locus_tag="ACV5NJ_001142"
     CDS             complement(7927..8631)
                     /locus_tag="ACV5NJ_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHY6485244.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(8643..10952)
                     /locus_tag="ACV5NJ_001143"
     CDS             complement(8643..10952)
                     /locus_tag="ACV5NJ_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HHY6485245.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(11147..11611)
                     /locus_tag="ACV5NJ_001144"
     CDS             complement(11147..11611)
                     /locus_tag="ACV5NJ_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485246.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(11611..12237)
                     /locus_tag="ACV5NJ_001145"
     CDS             complement(11611..12237)
                     /locus_tag="ACV5NJ_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HHY6485247.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(12244..12609)
                     /locus_tag="ACV5NJ_001146"
     CDS             complement(12244..12609)
                     /locus_tag="ACV5NJ_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HHY6485248.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(12599..12937)
                     /locus_tag="ACV5NJ_001147"
     CDS             complement(12599..12937)
                     /locus_tag="ACV5NJ_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485249.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(12927..13253)
                     /locus_tag="ACV5NJ_001148"
     CDS             complement(12927..13253)
                     /locus_tag="ACV5NJ_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HHY6485250.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(13231..13512)
                     /locus_tag="ACV5NJ_001149"
     CDS             complement(13231..13512)
                     /locus_tag="ACV5NJ_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HHY6485251.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(13514..14878)
                     /locus_tag="ACV5NJ_001150"
     CDS             complement(13514..14878)
                     /locus_tag="ACV5NJ_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HHY6485252.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(14891..15460)
                     /locus_tag="ACV5NJ_001151"
     CDS             complement(14891..15460)
                     /locus_tag="ACV5NJ_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HHY6485253.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(15432..16619)
                     /locus_tag="ACV5NJ_001152"
     CDS             complement(15432..16619)
                     /locus_tag="ACV5NJ_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HHY6485254.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(16683..18410)
                     /locus_tag="ACV5NJ_001153"
     CDS             complement(16683..18410)
                     /locus_tag="ACV5NJ_001153"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HHY6485255.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(18407..18859)
                     /locus_tag="ACV5NJ_001154"
     CDS             complement(18407..18859)
                     /locus_tag="ACV5NJ_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HHY6485256.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(18971..19351)
                     /locus_tag="ACV5NJ_001155"
     CDS             complement(18971..19351)
                     /locus_tag="ACV5NJ_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HHY6485257.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(19348..19734)
                     /locus_tag="ACV5NJ_001156"
     CDS             complement(19348..19734)
                     /locus_tag="ACV5NJ_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HHY6485258.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(19736..20014)
                     /locus_tag="ACV5NJ_001157"
     CDS             complement(19736..20014)
                     /locus_tag="ACV5NJ_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485259.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            20077..>20325
                     /locus_tag="ACV5NJ_001158"
     CDS             20077..>20325
                     /locus_tag="ACV5NJ_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HHY6485260.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSEN"
BASE COUNT         5675 a         4369 c         3177 g         7104 t
ORIGIN      
        1 cgtaacacac ttatggggca gtggtttttg gggaacgctt taaactcttc tttattatta
       61 tctcacaata taacccaaat gtctttctat ttaaaaatca aagtaaaact tttcaaatat
      121 acagaaatat aactatgtga aacatccttt cattaatcca taaaaggata cataaaaaag
      181 ccactcattt gagtggcaat gaagaaaagc tttagcttgt ataatactct tcaaaaaatt
      241 ctaacacaga acgattcaaa tggctacgtt aatgtaccct gtaggactcg aacctacgac
      301 cggacggtta tgagccgtct gctctgacca actgagctaa gggtactggt tgttgccaca
      361 taaagccata aacaatcaac cagtagaatg tgtggcaaca aacctgttat cgcatagctt
      421 ggagtgtgac tattatgggt gatagtgaag atatgcgata acatcactat tttatcgaat
      481 gatttttata gttgtcaata tagttatgta atgctcctca acgaggagct atttttatcg
      541 tttaggaata tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt
      601 tccctctgga tcattcgttg cccgcatcat gacatagact ttcttattag ggaagttacg
      661 catgttaaaa gatacatgat agccaacatt tccagaagta ttataagctt gatttacatc
      721 tgatctataa attccatcag ctcttactcg agctaattct ttcccagtat tgtaatccat
      781 aatgaagata tactcgtatt tatagttagc aatgtgccat ccagctacat gcaaatttgc
      841 attttcgatt tctccaaact gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg
      901 attagctgca ccagctcgtg ttggatcaat gacaggcttg ttttcagaag ttgttggatt
      961 ttcatcggta aatccatgag ctaaatcata tgctaatttt tctttactta cgcccatttc
     1021 agaaagataa ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta cccataaatg
     1081 cgatttgatt cccggttggt tataaggagt gtccaacgtt aatggaatac catatttcat
     1141 tgctgaatct ctagccaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc
     1201 atgtgtgtgt tgtaactcaa tctgcacagg actgttggca ttagcatacg aaccagcacc
     1261 gtactgtaca taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata
     1321 agcagtataa gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc
     1381 tgtttcatta gccgtttcat gcagtaaaat atactgatta tttgctactt gtgagctacc
     1441 ttcatttgcg cccaaattaa attcattgtt gatagtatag gcaaatccgt taattggcaa
     1501 taaaaaaaga gccgttaaca ggctcatcgc agtaatagta attttctttt tcatttgttt
     1561 cctccttctt cgctttcagc cgagaacatt ttgtaggttc gatttgatac acccaacaca
     1621 ctccctaaaa acgcgccaaa accagtaatg atgacaacac agatatctgt gtactgccaa
     1681 ttgagcgctt taccaactaa ccccacgaaa gtagctagtg cgggaataat taccagtgcg
     1741 aaccatttta gtacttcgaa cgttttatta ttcattttct tctctcccta aacaaagttt
     1801 tgatttgttg cgtgtgttcc accaattttt ctgtatgtgt atctaatctt tcatcgtgtt
     1861 tctttagttc ttcatgaatc atcaatcgat ctgatttgct cgattctaaa tctttagtca
     1921 gcaaatctaa attgtggctt acttttgaaa gagtctcagt aattttcgag aaagatgcag
     1981 caatcggtct aattactaat aaaatcaaag aaacgatagc ggttattgat cctgctatca
     2041 ttccccattc ccctaaatta atcatgtgac aactccttta ccttaaataa aaacgcatca
     2101 attaagatgc gctcttatct ttattaatga ttttatctgc ttcttcgtct gtaatgcata
     2161 atggaacgaa aaccattact tgttcgttag tgaaacagcc ccagtcatac attagtttga
     2221 tatcttcata tgaatacatg ttacttccca ccttgtaatt gttttttgat ttcttcgatt
     2281 tccttggtat tttgtacaga agcaagcatc gtttttgaat tcaactgcgc taaactttct
     2341 gacttttctt tcaataagac attttcttgc ttgattgcta cattgtttag cattgcttta
     2401 gaatttagct gtttcaattc atcattttct gccttcagcg tttcgtataa tgccttaata
     2461 ttagccaatt catctacgct atcaacagca ttcccttttt tctcttcttc agtagctaac
     2521 tcaacccact gttttttatc aaaatcaaat cttggtctcc agttaggaac tggtggttta
     2581 atctctgtac agttttcagg gatattttct tgattgttca aaatgatttg ctcgaatccg
     2641 taaggcttga ttgatttgta aactactttc attagatttc ctcccttaaa ttgaataagt
     2701 gataacaaat gaataagctg atccataact tgaatttctt ctccatttaa tggctccgtc
     2761 tgcgccaata gatagttgag cactgttcaa agtagaacgg tctattgacc caaccagttg
     2821 ttcaaaacta attggtgtcc gatagccttc tggaacagtt aaaataaccg aatcattccc
     2881 gccactactt tttccgttta aagccacaaa atatatagaa actgtttttc cttcacgata
     2941 aagctttgct gtccctgtat taccgttagt gacaaccaga tttaccactt catatctgtt
     3001 atcatcagtc gtcaaaacac gtttattatt gattgttaca gaacctgaaa aattcttatg
     3061 tcctgcaatg gcttggtctc catctgtctg caccaatgct tctttaccat caatagcttc
     3121 tacatgcgtt tttaagtatt tagcaactcc gtcttctttt aattgaacaa tatcagccat
     3181 taaaccgtcc ccactttctc aaatgtaata ttcgctaatc catcaagttt cactttatcg
     3241 gttgctgaca ttaaacctgc tgtcgtagtt gtagcattac ctggattttt ctgtgctcca
     3301 gctgcaattc catccaactt agttttatct gtggacgaca tcaacccatt tgctgtagtt
     3361 gtagctacag ctgtagttgt ggcatttatt ccaggatcac ctttatctcc ctttggtaaa
     3421 acaaaattaa atctagctgc agatgatgtt cctacattcg taacagaagc ggttgaacca
     3481 ctagaaacgg ttcctatggt aattgttgct gcttggccag gatcgccttt atctcccttc
     3541 accgttgttg gtttgccttc tatagcattc caatgagttt gtggataaac ctgtacaccg
     3601 ctttgtttta tttttacgat atctgtcatt ttctatacct ccccgatttt ttcaaaagta
     3661 aaatttggaa ttctttcgtt tgtgtaattt tctgcttgat ttacagcttc ttggaatttt
     3721 tgatctacat atgactgatt aacaccacca gtcccactac cacctgtaga actaattgtt
     3781 ccatcttctg caattgatat atttgcacct gcttttaata tttttagaga ttctaatttt
     3841 ccctttaatt cttcagagaa attgaagtct gtttgcttag ttgcagataa aacaccttct
     3901 tcagtaactt ctaagagttc cccaactttt atacctccta attgttcagg agtagcaatc
     3961 ggcaaaatat atgttccacc ttctccattt acgatctttt gaaacatttc agcagtaaga
     4021 ataccatcgc ttgtctcgct tgcataaggt agttcagtaa ttgcattctc taatcctaaa
     4081 tcagctttcg ttaaaatgac tgcaccatat ttaccattaa ccgaaagaac ttttgattgt
     4141 cctgatatca ttttttctaa gcctaaaact gcagaaacat gtgtaattgg cataaattga
     4201 cgtttaacac cagattcatc agtctccatc attctcttta ctttaaccat caaatcaccc
     4261 caactttctc aagagtgaaa acattctgtt ttggatcatc aacagttgca ataatcaaag
     4321 ctccttcttc aattggaaat tctactgttt caattttttc gacttcgtga ttatctgaaa
     4381 agagatcgtc ttgaagaata tcagtcactt caatttcacc gtattcaatc gtgaaaagtg
     4441 ctgctttcat tttttgatac aaataattta gatcggatag gaaacgttca gaaatcgatt
     4501 catggcgtac accttgaaga tctacacgtg catccattaa ttcggctaac attactccac
     4561 ctggatcgat agacttcaaa atatccttga ttgattcaaa ccatgacaaa taatctgatt
     4621 cttgtccttc tctccaatct tggaaactat tctcctgttc ttctctccag cgatcaaatt
     4681 cttctttcct ttcattcatc cagtccgtaa aatcgccttt attttcatta ataaaatctg
     4741 tcatatcagc aattaaatct tcaattgatt gccaataaga acccatttct ccttctgttt
     4801 ttgaaacagc attgatgaca aaataggaga agtcttgagt tgttccaatc aagtcttctc
     4861 ctttaaatat aatgaaattg gctgtttgtc gatgcaaaca ctgcatggaa tatttatcaa
     4921 aaatatattt gattttccct tttttagcat ccacaattct tgtttctaat tggactggat
     4981 attttccgcc aacaactgat tcaaaatata ccttacattc tgataaatca tatggaagac
     5041 cattttcgac aattgtagct tccataactt cagtgttttt attgccttgt cgaacttgaa
     5101 tcatccccac gtaattatag ggttctgttg tacttaatat gacattccac tttgccatta
     5161 aatcacctcc ctattttggt ggtatgacaa tggaagaaat tgcacttgca ctataatact
     5221 ggcgatctaa ctttccacaa atcataccta actctgtgtt ttgttcataa gtttgcatac
     5281 gaccattagc taagcctcgg ataacgccag tatgtccata agttccgtct gcaaaccatg
     5341 aacccacttg tccacctctt gcccaattaa taattgcacc aactactaat tgatcatatc
     5401 tagggttttg aatcactttc caaccaacag cagaccaatc ataggcaata ccaatatcag
     5461 atgctgcaga agtatttcct atcacatgag ttaaaccata ttttgttcca gcacccatgc
     5521 cacaaccacc cagatatcct gaatattctg cagacaagcc ataacactga ccattgccaa
     5581 ttcgttgtcc aatcaaagac tctaaatgtt ttagtcctgc ttctccagta gctcctcctg
     5641 gttttaaatc tttaaatttg ttataccagt taactgcata atcttgacgt tcaggatgtg
     5701 tcgctgccgg acgttcatag tttcgttcaa aagcatatgc tgcttgtctt ggatcagtac
     5761 aggccttaaa tccatcaacc gtcgtaggtt gtactacgcc catccattgt ccatttgtaa
     5821 atgtccaaat aagcaatcga acttgtgcat ctaagctcgt tattggttct ttaatacctg
     5881 cagcattaaa taagttttga acataaactt ttccatccca tgttgctgga ccaacaagag
     5941 gatatgaaga accatcccac tgaactaatc cgtatgctgg tccacctatt tgaacagtat
     6001 caggatcaaa agttcctcct gtttcttgtt gaatgttccc caatattcca catgcagatt
     6061 gtttcgtaaa tccgttatta cacaaaatat cgtatattct ccaagctctc ttttctgcat
     6121 ctgtttttaa ttcatcagga tacccaccag gtgtttctcc tccgccagat ggaccgccat
     6181 tttgtcctgg tatcacttct ttacctctaa cagtaagttt tccctgaata tctaaatcac
     6241 cgaaataata tgcttttccg tttcctaata acacaaatcc tttttctgca gttggagaaa
     6301 ttaaaatgta ttttccatcg ccatttgtac gtattaccaa agaattttcc tcaagaggtg
     6361 taggtgttac agcatttgga aatggatttc ctgctgaatc tgttgttccg atcgttccaa
     6421 tggattcttt tctattccag aattccatac cttttttggt tagctccatg atttttttgt
     6481 tttcattcca aatttgaagc gtgcctttta caagttttaa cacatcacca taagcattaa
     6541 aggaagtttc gaatacttct gcatttattg ttcctacttt gatgaagtca gcaacaattt
     6601 ctccttttga tgtgatagca ataccgaacg ggccatttac tccgttgtct gaataaccta
     6661 agccatttaa attccaacgc cacactcgct ttgcattcgc cacatttgga gtatccatga
     6721 taagaatctc tgatggagct ttttctggac gaaaaacgac atgcccacca gaattaccag
     6781 tgatccatgc cgtcgcattc aacacatttt gaactaacgt ttctgttcga ttgtcgattt
     6841 tctttttcaa ttcttgtgct tgagtattta ctgccgaagt gtacagtgat aaatcattac
     6901 ctaaaacaat atccttatat ttcccaagcg ttgggaaata agtgtattcg accatacgct
     6961 cttttatctc gatatctagc tctttcgccc taacatgagc gacatcacca aagtgtaaag
     7021 acgctagctc ttgatacatg tcaccgtatt cgagtgtgtg ttccaacgcc accatactga
     7081 ctgtatgagt agcttttggc tcatggatac gatcattgtc aaataaggtt ttgccccatt
     7141 tgatcaattc atcaactgtt ttacaatctc cgttttcacg tttggcaata cgtcgattct
     7201 cgttggttac tccatcaatt tctaaataac catattcgat aggttctttg tcttcatcat
     7261 aatcgttatc aggaacaccg ccaaccaaat acaaactatt gacaattgat tcttcatcaa
     7321 tttcttcttc gattgcttct aaattgatgc caaaatcaat ccgaaaacca ttatctgatc
     7381 caatctgttt taccagtttc aaatcatagt tatccatatc aagttccgcg ctagtcacgc
     7441 cagtcaaatt ttgattaccg ttatttgaac caatgattgc ttcaactggc gcgacttgtc
     7501 ttgctgtaaa ttggtgcgtt gtccctacat tcgaaagata gttaaatttt tgatcaaatg
     7561 ccaaactgtt tttaagattg gtcatgatct gagacccatt gccgttatcc gtaaacgact
     7621 tgacaataaa gttcttattg gccataaaac caatatgccg agctgttaca gaaacggagg
     7681 tcaaattctt tttgacatta taaatttcaa aatattgcca tgatccatct ggtaccttcg
     7741 cttttataaa atttcctttt tttaaataag agcgatactg tccgcttctt gaatagttac
     7801 cataaaaacg atattggcca ttcagcacac gattgatttc tggcaaatct tcccaatcaa
     7861 ctaacgacgc tccgttcacg ctcaaatcat ctggcatttt tttataagca taaataaatt
     7921 cttgcgtcac agatacacac tcctattcca aaaccgcact tctttaaatt ttccggatat
     7981 ttttacctga ttccattcag gctgcaatac tggccaatca ccacgcgtaa ataaattcaa
     8041 accttcttgt attgcttttc caagctgagt atcaaccacg attgttgcag ctaacgtatt
     8101 aagtattgtt aggcttttat caccgacagc gatcgttata tctcctccgt tcgattctat
     8161 ttccagatag ggatgtgcaa tttcgtcacc atgatcaaaa atatccataa cactagaatg
     8221 aaaaatttta ggtgcttcac cgatttttct tttgagtggc tgacaacgaa aagtaacatc
     8281 gaatgtataa aagaagcccc actcgttttc gaaagggact tctttatcca tgctgcagat
     8341 agcctctaga tacttgtctg gatcattatg tgtgattaat tggcttttac cagtgagcca
     8401 tcgtttgact tctctcagtt tcgaatgtgg aatagtgatc ccttctattt ccaaatcaaa
     8461 aggttcatag tcatcaaacg tctccgtcaa ttctccgctt cggccctgaa tcgtgtaagt
     8521 ttcgtatcgc ttgttcggca taatatctgg aagctctgcc tcaatgatgc aattcatatc
     8581 aattactgca ttccgatttt tccaaataaa atttggttca tctggattca taaattgtcg
     8641 actcaaatag gaacacctcc caaatcacga atagcctgtt tgtttgcttt agcaaattta
     8701 cgattcatac gatctaactc agatggattg tttgcatcga ctttgccaat gtgtatatgc
     8761 tgttcaatgc tacctccaga aactcttcca ctgatccctt tgcgtttttc ttcgtctgat
     8821 aatggagtaa ctgtggtttt accattcttg gcagtcagta actcaggtcc agcttcacca
     8881 acaatcgctt gtccattgat tagatgaccg ccttgtgcta aataaggcag ttttgaaata
     8941 gaaaatgatt ttccacctac accaggaacc cattttggta tctttatatt attcaatcca
     9001 ccaataaatc cattgatcaa tccaatcatg gcattaatag gagctttccc tacagcaaca
     9061 ataccatcga aaatacctcc aaaaatatca actagacctt gccatgcttt tgaccaattc
     9121 cctgtaaata ctccagtgat aaaatctaga aaaccactga aaattcgttt tccagcatta
     9181 taaaaattat tgaaatttgc tataacacca tcgaaaaatc caccaaaata accacttaaa
     9241 aaattaaata tttcaacagc tacatctgaa acacctttga agaaagaatt cactccatca
     9301 tgaaaccatt taacgttatt atatgcccaa attagtccag taactaatgc gccaatagct
     9361 ataactgcta atgcaaacca gcctcccgac attccaaata aaccagcaag tccttgccaa
     9421 actcctataa aacttttaac ttccccaacg atttttgtaa cagatcccat aagtgaacca
     9481 agaacgatta gtactggtcc aactgctgcc gctataccag caattgttat gatccaattc
     9541 tttgtatctt gatctagaga cccccaccat ttagagaatt cctgtaataa ggaagttgct
     9601 ttttcaaaaa aaggcaaaag gcttacttgg actgcttcac ctacgtcagc cattgctaat
     9661 tttgcattgt tcatagcttg atcggcttga tcaattgggt cgagagtagc atcgaatgtg
     9721 tctcctacag ctcccccact ttctccagca gtttttgcta aatcttccag gttcaaagta
     9781 cctctacgaa ttgcatcggc cattctagga ccgcctttag tcccgaatac ttctgctgct
     9841 gcattgatgg cttctgtttc agaacctgca ttttttacct tgtcttgtag ttcttctagt
     9901 ccttgactta aagattttcc gtctttcgca taagccactg ttgctttaga taaactactt
     9961 aaagctgcgc ttgaatcaac accagattgc tcaaattgtc caagtaaagt gaccccttca
    10021 ccaaagctca accctaattg tttaatttgt ggtgcgccat caattgcttt ttgcatcaag
    10081 tcgtctacag attgaccagt attctgcgat gttttcgttg ttacatcaag gacagaattc
    10141 aaatcatcat attctagtcc ataagcatca atagcttgtc gagcagatat tgctgattgc
    10201 gaaacatctg tatcattgat ttcagcgtac tgtaggagat aattggttga atcttccagt
    10261 tttttatcca tgaatccaaa ttgtgtattt acctcaccaa tagcttcccc aaccgtttgt
    10321 aaaggtaaat gagtatttga accaacgttt ttgaaagact gtgaaagtct atcagcttga
    10381 tcacctgttg ctccggtttt tgtaatgatg gtatcaagtg cctcgtcaac ttcaccaaat
    10441 gctgcaagtc ctgctgctcc tgccgctaca ataggcgcgg ttactccaac agacattttt
    10501 tcaccaacgc cttttacttt ctcacctgtt tcttcgattt tctggagttt ttttgctgta
    10561 tcaacagaaa cgtcaccttg ttctttaagt gcatcgttcg tctgttctaa tgctgtacga
    10621 agtttatttt ctccggtttc agaattgagt aattgtttat aaagtttttc tgattcagca
    10681 gaatattcac cggtttcttt gacagatttt tcatattcct ctctcagcat ttgagatcgt
    10741 tgttcagcca atcctaattg tttttctaat ttttgtttag ccgctcgcaa tttttctgtt
    10801 tgagttgcat ccttgtccat cgcagacact tggtttttgt actcggcagc ggctaagttc
    10861 atttctttgt tgatttcttt gattgttttc gaataactga cttcgccatt cgttttaaaa
    10921 ttaagaacga catcagattc tttaccagcc atttatctta gcgctccttt ctaccaccaa
    10981 ggcgatttat ccatagttac agattgaggt ggttcaaaat cggtgttttg ttgtaaccac
    11041 tgtaaataag atttgagcca caagttaggt gtagacttta aaaagaaact ctcactccaa
    11101 ttcaatagag tgagagcaac gtaaatataa aaactccaag gagttcctat ctcttccgat
    11161 tctttttgtt tttctttctt tttgcttgcg ctttttgaaa gtcttgtggc ttcttggatt
    11221 tttttaggtc ttccacctga aatgtctggc tagcaaaaat ttccatacag gtactataag
    11281 cagacaacac ctctccactc attcccaaaa atttgaaaat agtttctgga tcttcctcta
    11341 aaccaccagt acgcagcatg gcataaatca aggcacgcat gatttttaga tcactaggcg
    11401 ataagttagc agaagaaatt tgtccttctt ttttagatag catggcgttc atatcagatt
    11461 caaattgcga ataatctcca ccatacacat ctgctataaa ttccatcgtt tgcatagtaa
    11521 atgaaatagg gaattctgcg ccttgaataa ttacaacagc agaatttttt aagtcttcaa
    11581 cgtgaattcc ataatcagat aaccgagcca ttaacccgca cctccaactt tagccaatgt
    11641 tttccactgt tcttcatcgt atacaggttg agcaatgaat ttattaaata attctagaga
    11701 agctccgtct ctatttgaat caaaacttgc atacatcaca ttattgtatt tcagtccgat
    11761 ggaaaccaga tttgcagtta catcgtcaat ttttgtttct tcctctgcag tggcatactc
    11821 ttcgtcaatc acattggata gttgtgtatt aggataccaa actgcttttt taccgccatt
    11881 ttcaatattt ccaataaatc caaaggcgaa gtacggaaat tctcgtgcgg tatttttgct
    11941 aaacgttact ccgcttttag caactaatcc tttgatttca tccatcactt ctatcggaat
    12001 acccacgtga tccaacgcaa gttcatgttt tgtttctcgc gacacacgac gaaacatttt
    12061 gctggatgcc catttttcta atgctgttcc attccctttg acaccgattt ttgtggcaat
    12121 tggcaatcgt aaaacttcac tataagtcgg tgcagttcca acagaatctg gcgttgccat
    12181 catcgcaatt aaaatgtcgt ccaatccttc aaaataaaaa gtatcttgtt ttcccaatta
    12241 aaatcaccct tcccataaat caagtatttt tctagtcata atttcttcaa ttttttcttt
    12301 gttttgttca tacgtaccac ttgcaaaatg ttgggcacgt tgatttgttg tgccattttc
    12361 ggcaaagcgc cagtaaaagg ccgtctcttc aaattgaact ttcacttgat cgttttcaac
    12421 tactactttt atttgatcgc gcatatgttt cttttttaag agcgacttag gaatattagg
    12481 aagcaactgc tttagataaa agttagctgc ttcttctaaa gattccaacg ataatttttt
    12541 cggatcaact tttgaaaggt ttcctaaata atccgatatt tcagaaaaac cattcttatt
    12601 acttggcatt ttccacacac ctcacatatg tgtaatagtt ggttacggta tcgtcgttct
    12661 catcaccttg tatacctgta aaatctgaat atggaatgcc agcattttgc agcgcttgtt
    12721 caataacaac taaatcctgt tctgttccaa gtgtaaaaaa agagacttgg taataaggta
    12781 attttttata gactttaccg gatgccattt ttttgctgtt actcacattt gagtacacaa
    12841 tgtatggata catcgtccct aatctggctt tgtctctgaa cactgctaac tttgttgatt
    12901 tcagcgctgt tttcaattca tcaaagctaa tcgacataag ctaaactcaa ctccatttct
    12961 cttgcatcgg gattcgtata aatgcgagta atgttatacg ttacagaatc aattttgagc
    13021 gcacttaatt tctctgtgat ggatttatcc catctgactt taattcgtct gacaacgtct
    13081 gtcttggctt gctgtgataa atatttttct tgagaagtca caccgagttc ttcatagaat
    13141 attagacgct taaattcgta aattgtagtt ggacgatcgt ttccgtctgt tcctgttttg
    13201 atgtctagca attcggcttt ccatctgaga ttattggtct gtctcttcgg cattttgaat
    13261 cgctccttgc acgataaatg gcgtcatggc attcatagct ttgtcgagtt catcctctga
    13321 aactctatat tcataggcaa tgccggcaac catcaaaata agatattctt gttggccacc
    13381 agttgctgtt ttgacataat cttttgccat atttaaataa aaagagagca aagaatcatc
    13441 catgccctct tcaaaatgaa tatgtgattt gaatttttcc tctaaagaca attctttagt
    13501 ttgctcttcc atcttaacca ccaactggtt ttgtaatttc gtagcgatat actgccggtt
    13561 caaatggaga ataaaccaat tgaccatcta gcaagttgta aatttggaat ccaatttgat
    13621 ttttaccaga gaatttttca acaagttttt gaatttccaa ggcaccaata acttcttgaa
    13681 ttttaaatgc agaaaaatcg ccaaaatata aaactggcgt gtctggttca ccctttttat
    13741 ctgctgcatc tgtccaatcc acaggatagc caactaattg gtaaccaatt ccaccttctg
    13801 cttgtgtgaa tggccgcaac aaaggaaatc catcatctgt tttcattttt tcaatggcag
    13861 tcaaagcagc tcgattaata ataaagcgtc cctttttcat cacttctgtc actggtgtat
    13921 ttttaaattc gattaatgca tcatataatt tttgcccagc acctgaagca gttagatcta
    13981 aaggtttttt aaatgctaca gcctttttgg ctaatgcacc aggattttca tttccagcgt
    14041 catcaccatt gaacatataa ttgatttctt tacgcacata agcttttttc aattcttcca
    14101 caacaatatc ttcaactgga acaccagaca tttttaataa ttttttagtt actgttgcca
    14161 aagcatcgaa ttcggcagga ttaagcaaaa tttcatcaaa ctgaatagct gtttcagcaa
    14221 tatcagttga acgctctttc ttgtttacat tcgcatctgc tttcttcaca agaattggat
    14281 atttgacatc tcctgatgtt cgcaccactg ttccgtattt acgaagtaaa ttttcttctt
    14341 gagcataagt aataacttca gatgcaatta cttctgggac agtaactgaa ccgttgccag
    14401 cttcaatccc taaagctcga gcttctgctt cagaaatatt tccagctaca aaattagcaa
    14461 atgcttttcg tagttgttga tctttttgtt tcttggtcat ttttgcacgt gcctccaatc
    14521 cgttcttaat tgatccaagt aatccatctc gttgctgttg agtaatcatt ccagaacgat
    14581 tttctgaact atcttcactg tctgaatctt cttcttgatc gttgtcttca ccagaacggc
    14641 tttcgtctga atccgtacta ttcgattgat catctgtatt gtcgtctgtt tcatcagttc
    14701 cagaatctgc gcctaattcg tctttaattc cgttcaattc atcaataaca ctgtcaattt
    14761 cttctttcac ggcttctaaa tctgcttcgc gtaattctcc tgattcaact ttttcacgta
    14821 attcagtcaa tcgttgctca cgacgtgcct tcattttttt caataattct ttatccatgt
    14881 atttttcctc ctacgcttct agcgcttgat taattttttt gattaatttt tttctttctt
    14941 cgacgttttt ttctaattca tcacgacttc ttaatgcagc ttctgtatct tcgtatgctg
    15001 gcaaaggcac aatagaaacc tcgtataatt ccacttcatg gattgttcga agcattggtt
    15061 cagaattgta gtcccacgtt tcttcagtcg gcacaaaacc aaaactacat tggttgatgt
    15121 ctcctcgttc catagattta actaagtccc ttgctaccgt tgtgtccggt aaatcaactt
    15181 cgaatttcaa accacgttca tcttcttcaa ggcgaagggt tccgctttta gtacgtccta
    15241 gcacattgga ccaatcgtga ttgaacaaac aacgaacatc cgaattacta attgcctttg
    15301 agaatgcacc aggaacaatc acttcgctta agtcatccca taaaagtgtt tgactgttga
    15361 atacagcagc gtagccgcta atggtcctcg tatgagtttc ttcatcggaa cgtgttgaaa
    15421 gattggtgat gtcaatcgtg cgaatttcct tcttcttcat ttccatcacc tcctttcaag
    15481 tcttgatcat tcgttggtaa ggaatcatct gttgcatttt tctggccaat cctagataag
    15541 tcatttgaaa tatagatagc ttgtgtttct ggagtattct gtttaggaaa accaagcatt
    15601 tctgccacgt tatctggact tgtaatcccg gtacgaacga tgttgtagcc aatatttgtt
    15661 tttgttgagt aaggaacaaa atccaaaata ttaattttcc attccactct atagccagaa
    15721 ttaggcataa aaaaaagagc ggtgtaatgt tcgctcttgt ttttcaatat tggtttgatt
    15781 gctttgttgt gcagatacat catcgctttt tcaatatctg tcttcatcag cgattgatac
    15841 gtatcaacat tgattcctag aaattttcct aagtcttttt tgtaaacacc taaataattc
    15901 aaaattgccg cgtcatcaac aggacttttt aatgtctcga tggaatatcc ttttcccaaa
    15961 ggaatcatct taacagaatg atcactctca ttttgcgttc cttccagttg atccaatata
    16021 gctttgacaa tttttgtttg ggcgctatta tttggattga tgtgggcgtc tagtttaagc
    16081 atgaacgcaa gtaaaccgcc tttagtatat ttgtccgtca aaactttttc agcgctcaga
    16141 acgccttcta gagtgtttct tgcaagatca attattccag caccttttaa tgaatcagtt
    16201 ccgatgttct taatgtgtcg aatcatttga ccaggtattt tttgaccatt catttcaaat
    16261 tcttcttgaa gtcgttcatt gattttagtt gttacaccgt acgccaaatg aagctgatcc
    16321 ctatccgtta atgggaatgt ctcaccattg atcaataaag tatttgtttc caatttggta
    16381 aattcgaatc ctgtcaaata atcattggga ctcttcaaaa tatttagtaa gtgatgatct
    16441 ttgacttcgt tgccgtcggg accaataaca attggtgtgg ccaatgccac ctgattagat
    16501 atgtcttgaa ctaattcata aacatcagaa gattccatga tagatgaatc cgtcacatat
    16561 ctttgaccat aacgtgtgta gtgtccaaaa atatcctcga tatatccacg tttttccata
    16621 aaagaataga ctgcattcga taaccgatca cgtaatttca atttttttca ccgcctttct
    16681 atttatctat agatggaact taggtaatca tctaactcat ctgaatcaat atctgtcatt
    16741 tgattcatcg tttccttatg accacacaaa aacgccacga acccatcaat ctttttcttt
    16801 gattgacgtt tacttggcgc tttttgtcca ttgatgttag taattgctac aacgttcaaa
    16861 gtgcaataaa ggaataatgg attatcaaat tgaattcgtt tctcataaaa caaccgttcg
    16921 acatcgtcaa aaggagcatt caatacttta ggatattgag caacttcaac gcattccaat
    16981 cctaagtttt ctaatttctc aacaagtttg tcactcatcg ctggatcata attcacttgc
    17041 tgaatatcat ataaattcat gcagtcctct atgtactgca aaatctgatc ttgatcaatc
    17101 atttttccgt cgcaaaattc aacgaaacct tgttcagcca aatcactgta aggaacatta
    17161 tcttcttttt ctcgaaactc taaatcttca ttcggaataa aataaagttg ctttacttta
    17221 agaaccgctt taccatcttc atcccatgtt gggaagttta aagatacaca ggtcaaatct
    17281 cgtgtacgtg ataaatctaa accgatataa catggttcgc cacttaaatc accaagttct
    17341 tgtgtggtaa ctaaacatgg ctctacttga tcctgttcaa agaaattatc cgcgccgttc
    17401 acaaatacat ccaagtgttt cgttagaaat tcagctttgg aatgagctga ccgttgcgct
    17461 gttttaaatg ccgattctaa agcagataaa tcgactgata ttccccagtt aggattgcac
    17521 atttcccaga cttttctatc cgtccaatca taatttttat ttggctcata gattagaaca
    17581 aagtttgaat cattatcatc acgtttcaac acttcttttg cttcgcgata tacacgcatt
    17641 ccaacagacg acgaaccttt cccagcagtt gagatattaa acatcaatgg ctgtggtaaa
    17701 gatatctgag cagacttaaa attgtcatac tgttccattt tctcctgttt atgcaattca
    17761 tcgttcaata caaaatatgg attggatccc tcgatattat caatattctt ggtttgaaca
    17821 ataaatttat ttgagtaagc catatcttca tgaatatagt cataagtaat actagaaaca
    17881 gttccctttg gccccctaaa tattttagtt ccttcaagta ggattggatt gtttaagatt
    17941 gtaactgcaa aaggtttggc agcatattgc gcttgagcaa aatcagatgc gcaagcatag
    18001 caatcaactg ataacgcccc ttctccgtac attgcatatc ctaatgaacc gacagcaatc
    18061 aatgttttac cattcttttt tggaatctgg acataagcct cacgagtaac gcgtacaatt
    18121 tggccttttt cattttcttt aacccaacca taaatccaag aataaataaa tttttcccag
    18181 ggttctaaaa gaaacggttt tcccaccatt tctccttttg tgtgaacaat aaatgattct
    18241 acccaatcca tcatttcatt cgcgcgatct acatcaaacc aaatatcttt tctttttttc
    18301 cactgatacc agcgatctat tgctaaacga acggtcttag gatacttctt aggtttcttt
    18361 cttacttctt ttgcaaataa atcagcataa tttacaccag gttcaatcat ttttcagcac
    18421 ctgccttctt acgccattta tttctgtgcg ctgctagttc atctactggc tttacttctg
    18481 ggcgtgtaat ttcttcatct tttcttgcgg tcgatcctcc agcaattagt ctaccagttt
    18541 ttgctttgtt tgttaatcct aataaatcta gagctttcat cttcttatct gcccaagttt
    18601 ctacttgttg tgccaatgga tgttttgatt gattagtagc tcctgattta ttcgtgaatt
    18661 tttgcgtctc cggaaatcct ttttccttcc acaataaata tttgtgttgg taaatttcaa
    18721 aaatatccaa gtatgattcg attaatggat caagagtgat ggtgtacaaa tcagacaaat
    18781 tcattatttc taagatacga gctttttcag cacttacttt ttcatcaata atcgctttac
    18841 gttgcgcttt agtcgtcatt tttgtataca cccccctttg ttttttgaaa aatttgacct
    18901 aacgatgcgc gtgactcccc gctaccctat ctccccacgc gaaaaaattt tgaaaatgaa
    18961 taggggggct tcaattgaaa tacgaaggaa aaactttttt gtcttccgtt tcgttttcaa
    19021 caattggatg acattttgaa cataatagca tgagattgtt tggatcaagc ttaagcagtt
    19081 cgttgtcttt gattggtaca atgtgatgga cgtgtgctct ctttccaaat atgaactgac
    19141 cacaccgctg acaatgacca ccttctcttt cataaataaa ttgacgcata tctttccatg
    19201 cttgcgttcg atagaatggt ttgttctcat gatgataaac agactttgct tgctgcttct
    19261 tcttgcgtga tctgcttgat ctcttgtgtt cagtacagta gatacccttt gctattttgt
    19321 tcgtgcatcc gtcaaactga cagtatttca ttctacttca cgaataagat ttatgatatc
    19381 gccttttgca cggatagcac caggaatatc aataccatgt ttcttagcat atgcacgcaa
    19441 ttcttttgca gtcatattgt ccagttcatc cgtatcttca gtggactgat cattagtagc
    19501 ttcgtctcca tcaaaattag cagcagtgtt tccatcgcta tcaagaattt cactgctatg
    19561 agctatgagt tcgccattga cagcaacaaa tgatttgcca tcactcaaaa ttcctttttc
    19621 ttcttttact tcaaaatttg gttcttgatc tttcggaaca actactgtcc ttttcttttc
    19681 tgaatcccaa tactctgttc cactaatgga tgttctaatt ttgatcattg ccattttaat
    19741 tctctccttt gtaatttgta cggattactt ttgcgcccat tcgctcatac cattcaatcg
    19801 tttcctgcag attgggtaga gtgtgtgaca ataaagaaat aactaacgta ttcattccag
    19861 ttctgtaatc atcattctcc acttgcacat gtgcatgatt accattccaa ttttctctgt
    19921 acggactatt ctctgcttca attgctttct tataaacttt agccaaactt gattctactt
    19981 caatactaag taccgcttca tgaaaatcat tcatttgttt actctcctat cagatatttt
    20041 gtggtatgat ttataaaaaa gatgcgaggt gttttaatgc ttcaagaagt taatattaag
    20101 tatcgtgatc gtcaaaatac agagacaatt tatttgccaa atagttgtcc gcattgcgga
    20161 aatataatga ctccgtatgt gtattttggg gtttcatcat atagtgcgtt cgataatgat
    20221 agaatctttg gcgtattagt acagtgtata aatgatgagt gcaacaaatt ttatagttta
    20281 caatttataa atacgtttaa tgaagatgaa ggatattctg aaaat
//
LOCUS       DBKOIJ010000042        20250 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000042 DBKOIJ010000000
VERSION     DBKOIJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.042"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(36..1595)
                     /locus_tag="ACV5NJ_001159"
     CDS             complement(36..1595)
                     /locus_tag="ACV5NJ_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HHY6485261.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1750..3984
                     /gene="pcrA"
                     /locus_tag="ACV5NJ_001160"
     CDS             1750..3984
                     /gene="pcrA"
                     /locus_tag="ACV5NJ_001160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HHY6485262.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4001..6037
                     /gene="ligA"
                     /locus_tag="ACV5NJ_001161"
     CDS             4001..6037
                     /gene="ligA"
                     /locus_tag="ACV5NJ_001161"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HHY6485263.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6143..6448
                     /gene="gatC"
                     /locus_tag="ACV5NJ_001162"
     CDS             6143..6448
                     /gene="gatC"
                     /locus_tag="ACV5NJ_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HHY6485264.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6448..7920
                     /gene="gatA"
                     /locus_tag="ACV5NJ_001163"
     CDS             6448..7920
                     /gene="gatA"
                     /locus_tag="ACV5NJ_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HHY6485265.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7917..9347
                     /gene="gatB"
                     /locus_tag="ACV5NJ_001164"
     CDS             7917..9347
                     /gene="gatB"
                     /locus_tag="ACV5NJ_001164"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HHY6485266.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9357..10412
                     /locus_tag="ACV5NJ_001165"
     CDS             9357..10412
                     /locus_tag="ACV5NJ_001165"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HHY6485267.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10523..11896
                     /gene="rlmD"
                     /locus_tag="ACV5NJ_001166"
     CDS             10523..11896
                     /gene="rlmD"
                     /locus_tag="ACV5NJ_001166"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HHY6485268.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11899..12027
                     /locus_tag="ACV5NJ_001167"
     CDS             11899..12027
                     /locus_tag="ACV5NJ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HHY6485269.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12467..12655
                     /locus_tag="ACV5NJ_001168"
     CDS             12467..12655
                     /locus_tag="ACV5NJ_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HHY6485270.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12733..13746
                     /locus_tag="ACV5NJ_001169"
     CDS             12733..13746
                     /locus_tag="ACV5NJ_001169"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHY6485271.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13813..14847)
                     /locus_tag="ACV5NJ_001170"
     CDS             complement(13813..14847)
                     /locus_tag="ACV5NJ_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6485272.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            14962..17187
                     /locus_tag="ACV5NJ_001171"
     CDS             14962..17187
                     /locus_tag="ACV5NJ_001171"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HHY6485273.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17205..19088
                     /locus_tag="ACV5NJ_001172"
     CDS             17205..19088
                     /locus_tag="ACV5NJ_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HHY6485274.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19069..20043
                     /locus_tag="ACV5NJ_001173"
     CDS             19069..20043
                     /locus_tag="ACV5NJ_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HHY6485275.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6827 a         3713 c         4341 g         5369 t
ORIGIN      
        1 tgaatagttg ttgtaaaata gtttttccat gttcgttaaa atgattgctt caaatttgtg
       61 atagaagatt tagcctcgat ttcctgcttg attcttggca agtttgcttc atcagtcaag
      121 ataaccaaaa tatctcctac attcatgatc gtatcaccgt gagtcaagat ttcctgattt
      181 cctctgcgta tagagattag cagcatattt ttaggccaat tgaagtctcg taccattaat
      241 ccgtctaaag aactctctgc cgttactgga aaatcaaaag ttgtccttgc accttttagt
      301 gaggcggtct gagtatttcc aagtagacgt tccaataatg attcgtaaat tggattaccg
      361 ccgagaaggt cattgacgat ataagcaaca agagcagcca atcctaaagg catcagatga
      421 ttaagagagc caaccatttc tgtcactaaa ataattgctg tcagcggtgc ttttccaatc
      481 gctgtgaaat aaccagccat ggcaataacg acaaaatcct tgatgtagat tggatccatc
      541 ccgaaatcaa ccataatgtt tccataaaag ctaccgatta aagcccccaa tgtcaatatc
      601 ggaaggaaaa tcccccctgg aagaccccca ccgtaagaaa tcatggaaaa aacgaatcga
      661 agaacaaaca aaccgataat cataatgatt ggcaaggaag catgtccaaa agctaaaacg
      721 atttgattgc ctccgcctag agtactaggc cataaataac caattggcaa aatcagcaag
      781 aaaggaacga acccgtgaaa ataagctggt aaaggcaatc ggttgtaaaa ttttggcaaa
      841 gccagcaaga cattctgata aataaaacct aagatgccta gaagtactcc taagatcact
      901 aaggaaccat aatatttcaa cggcaaagaa ggaaggtcac ctagatataa aacaggtctc
      961 aagccgaaaa agtaaagaga gacgaagtta gcagtgattg ctgaagcaaa tgatgtcagc
     1021 caaacaagtg gagaaaagga gtgatggatt tcttctaaaa caaagagtaa tcctgcgata
     1081 ggagcattga atgctgctgc aagtccgcta cttgcaccgc tagaaattaa aatcttttct
     1141 tctgaagatg gactccgaaa caattgtcca gctccttgtc caatggatgc gccaagctgt
     1201 atagaaggtc cttctctacc tagaaaaagg cccgcaccaa ctgaaagtac accgccaatg
     1261 aactttttcc ataaaacaga gaaccagttc attgataatt ccccgcgaag ctgtcccact
     1321 acttgtggaa tcccgcttcc cttgatatcc ggttcatttt tgaccagata acccacgaaa
     1381 aatgctgcaa ggatgcagca aatagctagg ccaattaagt aaatcggctg ttcccgactc
     1441 atttcataga tacttcgaac taaatcgctt agcttttcga tagataatcg aaacaggcta
     1501 acgacgattc ctgtcagcag tccaacaaat attcctttta gaataaaata tatctgagtc
     1561 ttatctaatt tcttgatttt tccttttttt tccattcaaa cgcctcgctt tcttttaatt
     1621 tagcgcgaaa tcaacgaatt gacaaggaaa aatccataaa aagaaacaaa tgattttgaa
     1681 ctagctagtt taatgaaagc caagaaaatg ctataattaa caagattgat aaaagaaagg
     1741 atgttgttaa tgtcatccaa agctgaatta ttaaacggga tgaacccccg tcagaaagaa
     1801 gctgttcttc acactgacgg accattactg ctaatggcag gtgcaggaag tggaaagacg
     1861 agagtgctga cacaccgcat tgcctatttg attgaggaaa aagaagtcaa tccttggaac
     1921 atcctagcga ttacttttac caataaggct gcaaaagaaa tgaaagaacg tgtcaatgcg
     1981 attctcgcat ctggaggcga agatgtctgg gtatcgactt ttcactcgat gtgtgtgcgt
     2041 attttaagaa gagatgttga ttttatcggg tataaccgga actttacaat catcgattct
     2101 tctgaacagc tgaccttgat gaaacggata ctaaaagaac tgaacattga tccgaaaaaa
     2161 tatgatcctc gaagtatatt aggaacaatc tctcaagcaa aaaacagcct gcagacccct
     2221 caggattttg caaaaatgca aggcagttat tacgaagaga ttgcggcaaa atgttatgct
     2281 gcttaccaaa aagagcttca atataatcaa tgtatggact ttgacgacct gatcatgaat
     2341 acgattcgat tatttgaaga acatcctgat tcactcacgt attatcaaaa caaattccat
     2401 tatattcatg tagatgagta ccaagataca aatcacgcac agtatacgct agtcaattta
     2461 ttggcaggta gatttaggaa cctgtgcgtc gtgggagatg cagatcaaag tatttacggc
     2521 tggcgcggtg cagatatgca gaacatcttg gattttgaaa aagattatcc agatgctgca
     2581 gtaattttgc tagaacaaaa ctatcgctcc acgaaaaaca tccttagcgc agcaaaccaa
     2641 gtcattgaaa acaatagcaa tcgaaaacca aaaaacttgt ggacagaaaa caaagaagga
     2701 aacaagatca cttactaccg tgcagataat gaacgagacg aaacccgttt tatcgtagac
     2761 cggatgcaag aagagattcg aagcaatcat cggaattacg gcgactttgc catcttgtac
     2821 cggacgaacg cacaatctcg tgtaatggaa gaaactctgt tgaaagcaaa tattccttat
     2881 aaaatggttg gagggcacaa gttctacgat cgtaaagaaa tcaaagatat tttagcgtat
     2941 ttgaatgtgc tggtaaatcc tcaagattca atcagttttg agcgtatcgt taattcgccc
     3001 aaacgaggca tcggaccagg atctatcgaa aaactacgca gttttgcttc tcttcatgaa
     3061 tggccactgc ttgaagcagc gcaaaatgtg gatctggcaa atatcggtgg aaaagcagga
     3121 cagcaattag gtgcatttgg agagatgatc caagaagtca ctcagatgat cccatacttg
     3181 acagtgacag aattgacgaa agaggttttg gatcgtagcg gatatctaga agacttaaaa
     3241 atccaaaata cattagaagc acaagcacgc atcgaaaact tggaggaatt tttaacggtc
     3301 acacaagaat tcgacaaaca attcgaacag caaaatgaag aagacgctga tgcaccagaa
     3361 gaaaaactaa cggtcttctt aaatgatctg gcacttgttt ccgacattga caatttagaa
     3421 gaagatgctt cccaagtgac attgatgact ttgcatgcag ccaaaggatt agaatttcca
     3481 gttgtattcc tgattggtct agaagagggg gtttttcctt tatctcgagc attgatggaa
     3541 gaaagcgaac tggaggaaga acgccgtctc gcatatgtgg ggatcacgcg agcagaagaa
     3601 gcgttgtatt taacaaatgc tttctccaga acattatatg gacggacgca atataaccga
     3661 ccttctcgtt ttgtagaaga aatcgatcag gagttactgg aaatcgaagg catgcgtcct
     3721 acgcctaaaa aaacgcctgt attcgctaaa aaaacagcat atagctataa acagccagag
     3781 acagcggttg ttccctctaa aagtgctact ggaggggaaa ataacagctg gaaacctgga
     3841 gataaagtca aacacaaaaa atggggtcta ggaacagtcg tccgagtaag cgggacgtcg
     3901 aaggatctag aactagatgt ggctttccca agtcaaggag taaaacgtct tttggctgcc
     3961 tttgcgccaa ttgaaaaagc ttagtagtgg agggtaaatg atggaacatg aaatgacgat
     4021 tgacgaagct gctaaacgag cagaagaact tcgtacaaga ctgaaccaat ggtctcgcga
     4081 atactacgta gaagataaac caacagtgga agactatgtt tatgataaag aatacgcaga
     4141 attagtagca atcgaggaac aatatcctga tttgatcacg tctgattctc ccactcaacg
     4201 agtaggcgga aaagtattag aaggttttga aaaagtcact catgatattc cgctttacag
     4261 tttgaacgac gttttcagca aagaagagct gatagctttt gatcaacgtg tacaaaaagc
     4321 agtgggacgt gtagttgact attgctgtga actaaaaata gatggacttt ccgtttcttt
     4381 gcgttatgaa gatggaaatt tcgtgagagg tgcgactcgc ggggatggaa ctgtaggaga
     4441 aaacatcact gaaaacctaa aaactgtccg gtcggtccca atcaaattaa aagaaccaat
     4501 gaacatcgaa gtaagaggcg aatgtttcat gccaaaacgg tcatttgtcc aattgaacca
     4561 agacagagaa gcagagggaa aagatatctt tgcgaatcct cggaacgccg cagctggttc
     4621 tttgcgtcag ttagattcta aaatcacagc gaaaagaaat ctggacactt ttttatacac
     4681 agtagctgat ttcggtccta tgcaagcaaa aacccagtat gatgcgctag aagagctaga
     4741 aaaaattgga ttccacacga atcgggaaaa acgtctatgt cattcgattg atgaagtgtg
     4801 gtcatacatt gaagaatatc atgacaaacg tgtggatttg ccctatgaaa tcgatggaat
     4861 cgtcatcaaa gtgaatgaat tctcgctaca agatcaactt ggttttacta tcaaagcacc
     4921 gagatgggca gcagcttaca agtttccgcc agaagaagtt gaaacgctaa tcgaaaacat
     4981 cgaatggaca gtcggtcgaa caggggtagt aacgccaaca gctatcatga caccagtccg
     5041 agtagcagga acgacagtaa gccgtgccag cctgcataac ggtgattata ttaagttgaa
     5101 ggatatccgc ttgaaagata cagttctgat ttataaagcg ggagacatca tcccagaagt
     5161 ttcgcaagtc gtgttagata aacgacctaa agacagcgaa gaataccaat tgcctacaca
     5221 ttgtccagtt tgtggaagtg aacttgtcca tttggatgaa gaagtagcct tacggtgcat
     5281 caatcctaaa tgccctgctc aaatgaaaga aggtttgaac cactttgttt caagaaatgc
     5341 gatgaatatc gatgggttgg ggccgcgcgt actagaacag atgtatgata aaaaactagt
     5401 agcagatgtt gctgatcttt acaaattgac agaagaagag ttattgacgt tggacaaaat
     5461 aaaagaaaaa tcagcaaata atattttgac agcaatcgac aacagcaaag ataattcggt
     5521 cgagcggttg attttcgggt taggaatccg acatgtcgga gcgaaagcag caaaaatttt
     5581 agcagaacat ttcggtgatc tagaaacatt gagtaaaagt gactatgagt cgattattgc
     5641 gcttgatacg atcggtgata tcattgccga cagtgtagtc acttatttca gtaacgaaga
     5701 agtccatgaa ttgatgaatg aattaaaaca agcaggcgtc aatttcgaat acaaagggct
     5761 tcgaaatgcc caattgcaag aagtcgagtc tccttttaaa gaaaaaacag tagtccttac
     5821 tggtaaacta acacgattta ctcgtgaaga agcaaaagag acgatcgaaa atcttggcgg
     5881 gaaagtgact ggtagcgtat cgaaaaaaac ggatatcgtg gtagcaggag aagatgctgg
     5941 cagcaagctg accaaagcac aagaattagg aatcgaagtt tggacagaag atcaaatggc
     6001 agatgcattg gctaaaagtc gctcagtaga agaatgaaaa atgagttttt tcagacaaat
     6061 ttcaataaac aatttcagac acggcactct tgtgttaaaa tagagagcaa agtttttaac
     6121 tcgtttagaa agaaggggta gtatggcgat tagtgaagaa caagtgaaac acgttgccaa
     6181 attagcaaaa cttgcgttcg cagatgaaga acttgcaagt tttaccgacc aattaggtaa
     6241 aatcatcgat atggttgaaa tgctggaaga agtagatacg gaaggtgttc catttacgtc
     6301 gaatgtggtt gaaacagtaa acgtgatgcg acaagatcgg gcgcaagctg gatggaaccg
     6361 cgatgaatta ttgaaaaatg tgccggaaac agaagacggc tttatcaaag tacccgcaat
     6421 catcgataat ggggaggctg gcgcataatg acagaattac acaatcgttc tctagaagaa
     6481 ctgcatcata tgcttgtatc gaaagaaatc actgtacaag atttgacaaa aacaacattt
     6541 gaacgaatca aagaaacaga gcctgaggtc gaggcattca ttacattgaa cgaagaaaaa
     6601 gcattggaac aggcaaaagc tttagatgaa aaagggattg atgaaagcaa cgtccttgct
     6661 ggtattccaa ttggtatcaa ggataatatc gtaacaaaag atatcttgac aaccgctgca
     6721 tctaagatcc tttacaattt tgaaccaatt tatgatgcaa cggtcatgga taaaatctat
     6781 caagcagata tgatccctgt tggtaagcta aacatggatg aatttgctat gggtggaagt
     6841 acagaaactt cttatttcaa gaaaacaaaa aatgcttggg acccaacaaa agtacctggt
     6901 ggctcttctg gtggttctgc agcagcagtt gcagctggac aaattccagt ttctttaggt
     6961 agtgatactg ggggaagtat ccgccaacca gcagctttta acggcatcgt agggatgaaa
     7021 cctacttatg gccgtgtctc acgttttggt ttgattgcct ttgcttcatc tcttgaccaa
     7081 atcggaccaa tgacccgtac ggtgaaagat aatgcattag cattgacagc tatcagcggt
     7141 tatgacgaaa aagacggtac ttcttcaggc gtttcagttc ctgattttgc agagggactg
     7201 acaggcgaca tcaaaggaat gaagattgct ttacctaaag aatacttagg cgaaggtgtg
     7261 gcccctggcg taaaagaagc tgtattgaaa gcagcagaaa cattccgttc attaggtgct
     7321 gttgtggaag aagtcagctt gcctcattca aaatacggtg tggctgttta ttatatcatt
     7381 gcctcatcag aagcaagttc caatttgcaa cgattcgatg gtatccgcta cggttatcgc
     7441 tctgaaaatg tgaaatcatt agatgatgta tatgtcaact cacgttcaga aggattcggt
     7501 gaagaagtaa aacgccgtat catgttaggt acattctcct taagcgcggg ttattatgac
     7561 gcttacttca aaaaagcagg acaagtccgt acattgatca aacgagattt tgaaaaagtc
     7621 tttgcagatt atgacttgat catcgggcca tcttcaccaa cagttgcatt cggaatcggt
     7681 gagaacatta acgatccaat cacaatgtac atgagtgaca tcttaacgat ccctgtcaac
     7741 ttggctggac ttccaggtat gtctgtaccg gcaggtttat cagaaggatt accagttgga
     7801 ttgcagatca tcggtaatta ttttgatgaa aaaacgatgt atcaagcggc atatgcgttt
     7861 gaacaagcaa cagaattcca taaacaacaa ccagctattt taggagggaa agacgaatga
     7921 attttgaaac agtcattgga ctagaagtcc acgtcgagtt aaaaaccaac tcaaaaattt
     7981 tctctccagc gccagctcat tttggtgcag aaccaaacag caatacaaac gtgatcgact
     8041 ggggttatcc aggcgtgctg ccagtaatga ataaagctgc gctggaattc gggatgaaag
     8101 cagctttggc attaaactgt gaaatctcta aagatactca ttttgaccgg aaaaattact
     8161 tttacccaga taatccaaaa gcatatcaaa tttcccaatt tgatcaacca atcggtcatg
     8221 acggatggat cgaaatcgaa gtagaaggta aaaagaaaaa aatccggatc gaacgtgtcc
     8281 atttagaaga agatgctgga aaaaacatgc atggtattgg cggttattct tacgttgatt
     8341 tgaaccgaca aggcacgcct ttgatcgaaa tcgtttctga agctgatatg cgttcaccag
     8401 aagaagcata cgcttatcta gaagcattgc gatcaattat ccaatttaca gaagtcagcg
     8461 atgtgaaaat ggaagaaggc tctatgcgct gtgatgccaa tatttctctt cgcccttatg
     8521 gacaagaaga gttcggtacg aaagcagaat tgaaaaactt gaactcttta tctttcgtga
     8581 aaaaaggttt agcttttgaa gaaaaacgtc aagcaaaagt tcttctttca ggaggagaaa
     8641 tccaacaaga aacacgccgt tttgacgaaa caacgaataa aacagttttg atgcgtgtca
     8701 aagaagggtc tagtgattat cgttacttcc cagagcctga tatcccgaaa ttcgtgatcg
     8761 atgacgaatg gattcaaaaa gtaaaagcta gtttgcctga gatgccagct tctcgtcgcg
     8821 aacgctatat ccgtgagttt ggacttccag agtatgacgc aatggtattg acacttacaa
     8881 aagaaatgtc tgattttttt gaagctgttt tggaaaatgg tgcagatgcc aagcaagcat
     8941 ctaactggtt gatgggtgaa gtttctgctt acttgaacag tgagaaactg gaattagctg
     9001 agacaaaatt gacacctgag aacttagcag gaatgattcg tttgatcgcc gatggaacga
     9061 tcagctcaaa aatcgccaaa aaagtcttcc gtgaattgat cacaaatggc ggagaagccc
     9121 aagaagtagt tgaaaaaaat ggttgggtgc aattatctga tccgaacaaa ctactgccaa
     9181 tcattaatga aattttggat aacaaccaac aatctgtcga tgatttcaaa aatggaaaag
     9241 atcgtgcggt tggcttccta gtcggtcaaa tcatgaaagc aactaaagga caagcaaatc
     9301 ctggtgtcgt gaataagcta cttaaagatg aattagcgaa acgatagagg ctgaaaatga
     9361 aaaaagcacg cgtaatttat aaccccacct cgggaaaaga attattgaaa aaaaatttgg
     9421 cggatatcct acaggcatta gaagaagcag gatttgaagc cagcgcctat gcaactacac
     9481 cagaaccaga ttcagccaag aatgaagcat gtagagtcgc agaactaggt tttgatctag
     9541 tagtggcagc tggtggcgat ggcacaatca acgaagtggt taatggaatc gctccgcttg
     9601 aacaccgtcc taaaatggcc atcatcccag ctggtacaac gaacgactat gctcgagcat
     9661 taaaaattcc tagagataat attaaagcag cagcagaagt gataaaaaaa caccaaaccg
     9721 taaaaatgga catcggcctt tctgataaca gttacttcat caatatcgct gcaggtggct
     9781 atttgacaga attgacttat gaagttccat cagaactaaa gagtattttt ggttatcttg
     9841 cttatcttgc gaaaggagca gagatgctcc ctcgagtaaa gccaatcaaa atgcgcttga
     9901 agtatgatga aggggaatat atcggaaatg cttcgatgtt tttcctaggg ctgacgaact
     9961 ctgttggagg attcgaacag atcgcgccag atgccaaatt ggatgatggg aagttttctt
    10021 taatcattgt gaagacagca aatatctttg agatcttgca tattgcagca ctcatgctga
    10081 atggcggtcg acatgtcgat gatccaagaa taatctatac caaaacaagc agtttgttcg
    10141 ttgaaacaga agatgatatc aatcgaccag taatgatcaa cttagatggt gaatacggcg
    10201 gggaagcacc gatgcatttc caaaatcttc atcaacacat tgagttcttt gccaataccg
    10261 atcagattcc tgatgaagca atcacaggta cagatgaaga agaattagag gttgtcagca
    10321 aagaattcgt aaaagaagtc gaacgtttaa cggatgaaga tatcgacggt gacggaaaaa
    10381 tagcagacga aacacctaat caaaagaatt aatcaaaagc cggtgctgaa cgtagtaagg
    10441 tcagtgccgg ttttagttgt gtaattagct gatttatagt agagttcagt aggattgaaa
    10501 aataggctta aaggagcaaa atatgacaga acagcaaata gtaaagaaaa acgaacgata
    10561 tacagtcgaa atcgtcgact tgagttatga aggaatgggg gtagcaaaaa tcgatggtta
    10621 tccattattt atcgaaaatg cactccctgg agaagaagta gaaatactag tagtaaaagc
    10681 aggcaataag tttggatatg gaaaagtcga aacatttatt acacaatcac ctgatcgtca
    10741 gcctgtagaa aacaatgcat tacttcgcat aggtatcgca cctttagccc atttgaagta
    10801 tgaacaacag cttgtcttca aacaaaaaca agtagagcat gtaatggcga agattgctaa
    10861 gatgccagaa gttcctgtac ttccaacggt cggaatggca gaaccattcg gctaccgtaa
    10921 taaagcacag atccctgttc gccgtatcga tggacagtta gccaccggtt tttacaaaaa
    10981 gaatagccat gatctagtcc ctatcgaaga tttttatatc caagatcctg tcatcgatca
    11041 agcaatcaaa gtagtcagag atatcttgca acggttccaa gtacgaggat ataatgaaga
    11101 aaaaaatgaa ggacagcttc gtcacatcat tatccgtcgt ggtcattatt cccatgaaat
    11161 gatggtcgtt ttagttactc gtaaagaaaa attctttaaa tccaaggaaa ttgctgaagc
    11221 tattcatgaa gaattgccag aagtggtttc ggtcatccaa aatatcaatg aagaaaaaac
    11281 aaatgtgatt ttaggagaca aagaaaaagt tttatatggc agagactata tcgaagatca
    11341 gctattagga aaaacctatc gaatctcagc aaagtcattt tatcaagtaa atactcaaca
    11401 agcagagtat ctgtaccaaa cagccatcga gtttgccgag ttagataaaa aagacattgt
    11461 gatcgacgct tattcaggaa taggtacgat tggcttgtct ttagcagaca gagtggataa
    11521 ggtgtatggg atggaagtta ttccagaagc aattgaagat gcacagttca atgctttaaa
    11581 taataaaatc gaaaatgttc attatgaagt cggaaaagca gaaaaagtta tgaaaaaatg
    11641 gcaagaaaaa gggatcaaac catctgtgat cgtagttgac cctccacgaa aaggattaga
    11701 tcatcatttt attgaatcag caatcgaaat gtcaccagaa cgaatcgtct atatctcatg
    11761 taatccggca acttttgcaa gagatgctaa gttatttgca gaagcaggtt atgccgtgaa
    11821 gaaagttcag cctgtagatc ttttcccgca aacccatcat atcgaattag tcgcttcatt
    11881 cgttaaagga gaataaaaat ggaccaaaaa gaaaaaatga aagaattgat caaaaagaaa
    11941 caaggtggag gccgatccaa acaaatggaa acacctaaga atgatcgtaa aaacatgaga
    12001 aaaggcccta aaatctttaa taaatagacg tgctaaagaa agactaagaa gtgttaactt
    12061 ttgttattgt ttcttagtct ttttagttag tctacattat tttggcgatt tgccaataag
    12121 gactgttgag ttgtgcaaaa tcaaatctgg gtcagaatga acgtcattct gtctcagatt
    12181 tttcgttatt tcaatttgat taactgattg atcaaaaaga tttttatttt catattctca
    12241 aatgattcaa cactaaatat acatgtttga cgcggaaatt attattcgat tttggcggca
    12301 tcagggggga tgacgacgta gcagacccat tattttactc aagaaaatgt ccgtgcctat
    12361 tatttagaat acgatagtga acgtgctggg agttttgttc ttttagagaa agtctctgaa
    12421 gataaattag ttgtcttaga ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca
    12481 gcaagtgatt gaccggatct acgaagcgac acgatatctg cctttggatc gtttgtgctt
    12541 aagtcctcaa tgtggatttg cttcaacaga agaagaaaat gttttgtttg aagaacaatg
    12601 gaaaaaattt cttttgtcaa aagcattgca gaagaagttt ggggaaaatc attaaatcat
    12661 ctaggagagc ttgataggaa ataacactat ggtataatca gaatgcttaa tactaactac
    12721 tgagggagcc ttatgaaaaa gaaaattctt aaatttatcg gatggttctt cggaatcatt
    12781 cttgggatcg cgtttgcaat ctttcttgcc ttccagcttt cccccagacc tggagcattc
    12841 ttgatcaacc atatgttttc aaatactgtt caaatcactg ataaaaaaac gttcgatcaa
    12901 gcagaaaaaa acgtggcgaa aaaaacagat ttgacgtatg agtccaagta taaagagaat
    12961 acttatgaca tctactatcc aaaaaacagc aaggggcctg ttcctgttct attttgggta
    13021 catggtggag gattcgtcgg cggagataaa tcgggtgtca aagaatttgc gacaaaatta
    13081 gtttcagacg ccaacatcgc agtggttgcg atgaattatg aattagcgcc tgattcagaa
    13141 taccctaatc aagttcttca agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa
    13201 gacgacaaat tgctagacat ggaacaatta ttttttggtg gagatagtgc aggttcacaa
    13261 atcgcattgc aatatgcagc tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc
    13321 aaacagctac ttccaaaaga tagtctgaaa ggaaccattt cttattgcgg acctgttgat
    13381 ttgaagctaa cagcacagca gcattcggat gatcgattta tgaagttttt cgtcaaaacc
    13441 gttgcgtggt cgttgattgg aacaaaagat tggaaaacga gtgagcaatt gaaagaagct
    13501 agtgtggctg accatgtttc taaggaattt ccgccaacgt atataactga cggaaatgca
    13561 tactcattcc aggagcaagg tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc
    13621 gtgcaatcct tgttttatac agatacaaaa aaagagatta cccatgaata ccaatttgat
    13681 tacacgctaa aagaatcgca aaattgttac cagcagacat tggattttgt gaataagtat
    13741 aagtaagagg ttgtgaaaaa gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc
    13801 acaacctctc tttcaatacc tttttgttct tgggataaaa ctttccctaa caatcaatcg
    13861 tgcaggataa gtcaaaatga ttggttcgtc ccgtataccg tcaattcgct ccaatgccat
    13921 tcgaacagct gcttgcccaa ttgatttcgc attgatttga acactggtca agccagggga
    13981 gaggtttgcg attgtctcga tattatcaaa gcttgctaca acaatctctt ctccaatttg
    14041 gatatttttt tgtttcaatg cccgatagac accaattgcc attggatcgc tcccgacaag
    14101 aatggcagta ggaagagggg attcattttc taatagatta gtcgcaagta cctcaccttg
    14161 tttttctgtc cagccaccta gatgataaga catatatttt tctaagtgat gatcgatcat
    14221 aaaatgctga taggcacgca tcctcttttc atttatagaa atctgcttcc caccgaattc
    14281 atccatatcg actttatatc ccccgatata ggctattctt ttatgaccat tttctaagaa
    14341 caattccagt acggttttag tcatccgttc aagatctgca tacaccatat cgacctcttg
    14401 ctggatgtca gggttatcca cgacgactat ttgcatgttt tgttttaaca aggcggtaat
    14461 cgattctttc gtaacagtcc caacaacgat caccgcacct aactgatcta gatcttccca
    14521 ttgtttagga ttatcagata gattatagat tcgattcatc cctaaatgca gacgtcgagc
    14581 ttcttcttcc atccctactc gcaaatgttt gtaatagata tcctgttctt cgctcgcttt
    14641 gccgatcgta gagatcacac caatcgaagg cattctcttt ttgatatatt tacgagtact
    14701 gatgtcataa tccaacgcag cagctgcctc tactatccgt tgccgagtag tctgagccac
    14761 atggatcgtc tgatcgttat tcaacacacg ggaaactgta gcaggagaga catttgcctt
    14821 tcgtgcgaca tctctaattc caaccatttt tacacactcc tcaaaaaaat aaaaaatttt
    14881 tattgacaaa attgtttagt aaacagtata caggaattat aaaaaaaagt aaacggtttc
    14941 cgttttgttg ggagtgaaat gatggatatt acgcatagaa aaacagataa aaatttcatc
    15001 ttatttgatg aaaagagccg agtcttccat ttacgaaata catttctttc ttatctgata
    15061 aagatagaag aaagcaatgt attggcgcat atttattttg gcaaaccagt caagcagtac
    15121 aaagacaata aaaattatcc ccgaagagat cgtggatttt caggaaatgt tcccttaaat
    15181 cctgaccggt ctctgtcgaa agatacacta ccgcaagaat actcttctca tggcagcatg
    15241 gactttcgaa cgcccgcatc gatcattcag cgcaagaatg gatcagactt actggatctg
    15301 agatatgaca gccattatat tacagatggg aagccagaga tcgaaggctt gcctcaaact
    15361 tatgtgatgg acaagtcaga ggcacaaacg ttggtgattt cacttaagga tagagaaaca
    15421 gcgatttatt tcgatttgtt ctataccata tttactgata gagcagtgat cacacgatct
    15481 gtaaagataa gaaatgaaac cggagaaaca atcaaactag aaaaagcggc tagtttccag
    15541 ctagactttg cccatactag gcgatttgat gaagtcattg ctttgccagg agcacatgtc
    15601 aatgaaagac agatttcacg gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt
    15661 cgaggaacga gcagtcacca tatgaataac tttattgctt tagttcatca tcatacgaca
    15721 gaaaatactg gagaagcgat cggacttcaa tttgtttatt ccggcaacca ttctttcgaa
    15781 cttgaaaaag atcagatcaa tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt
    15841 tcgtgggaat tgaatgcagg ccagtctttt caaacacctg aaatgatcct ttcttactca
    15901 agtcagggat tgaacaaaat gagtcagatc caccatgagt tgctacgtga aagaatcgca
    15961 agaggtcgtc atcaatttgc tgaacggccg attctggtca ataactggga agccacttac
    16021 tttgacttca atagtgaaaa aatcaaggcg atcattgatg aagcaaagga gctaggcatc
    16081 gaaatgttcg tattagatga tggctggttt ggaaaaagag atgcggacaa ttcttcatta
    16141 ggagactggt tcgagtatga aggcaagttg acaaatggat tacgtgaaat agcagattat
    16201 gctcatagca aaggattgaa atttggttta tggtttgaac ctgaaatgat ctcggttgat
    16261 tctgaactgt atcgaacaca tcctgatttt cttatgcagg agccaggtag aatgccttct
    16321 gcttccagat cacaacatgt actggatttt acaaggttag atgttcggca gacgattgag
    16381 aaacaaatga gaaagatttt agatacgatt cctcttgact acatcaaatg ggatatgaac
    16441 cgttcgcttt cagatgtcta ttcgattacc cttgacccac agagacaagg agaagtagcg
    16501 catcgttata tgctcgggct ttatgaacta cttgaacatt tgacaactga ttatccggaa
    16561 atcttatggg aaggctgttc tggtggaggt ggacggtttg atgccgggtt tatttattat
    16621 atgccgcagt catggacaag tgataacacg gatgcagtgg agcgtatgaa aatccaatat
    16681 ggaacaagcc ttgcttatcc gatttcgtcc atcacagctc atgtatcagc tgtaccaaat
    16741 catcagaccg acagatctac ttcgttgaaa acaagaggag aaacagctat gagcgctgtt
    16801 tttggttatg aactggattt gacaaagctg tctcctgaag aagaaaagca agtaaaagac
    16861 caaattatat cctatcaaac gattcgtccg gtgatccagt atggccatta ttatcgtttg
    16921 gcgagtcctt ttgaagaaaa tattgccgca tggatgtttg tgtctcctaa acaagatgaa
    16981 gctattgtgt ttttagggag gatactagct tcagctcaac cagcgttcca tgaagtatat
    17041 ctgatggggt tagatgatga ggcactttat caggaacaga cctcgaagcg gatattttcg
    17101 ggggccgaat tgatgacagt tggactttac ttccccgatt ttcaaggtga tttccaaaca
    17161 gaactgcttc atttcaaaaa gttatgagag agaaggaaaa aagtatgaaa gcaaatataa
    17221 aatggttaga tgatccacaa gtttttagag tgaaccaatc aaatgcacat agcgatcatg
    17281 tttgttatgc aacaagggaa gaagtagaaa ccacatcctc ccttaaacag tctttaaatg
    17341 gatcatggcg tttttgttat tcgccaaatc cttcggtacg accaaaaaca ttttataaaa
    17401 aagattttga tgcctgttct tttgatgaaa tcactgttcc tagtcatatt gaattagcaa
    17461 attatgacaa gatccattac atcaatacga tgtatccgtg ggagggacat gtatttcgcc
    17521 gtcccccttt cacgacaagc cctgagaaag aggcggaagg gatcttttca gctgctaaag
    17581 acaatccggt tggttcgtat ttgacgactt ttcgattaga agaaggtcta aagggaaaac
    17641 aaatctcgat tcattttgct ggtgtagaac aagcaatgtt tatttggttg aatggtcatt
    17701 ttatcgggta tgccgaagat tcgttcactc cgtctgaatt tgatttaaca ccttatttgg
    17761 aagaagaaaa tattctagct gtggaggttt acaaaagaag tacagctgca ttcttggaag
    17821 atcaggactt tttccgattt tttgggatct tcagagatgt cttcttagtt gctcgaggac
    17881 aaaaccattt agaagatctt tggatcaaac ctcgcttaga tgacaaatgt caaaatggca
    17941 aacttgaagt agatttgtct tttgagcaag tagccaaaaa cagtcagctg aatgtagcga
    18001 tactagatcc tgatgggaag gaaatctatc aacagcaata tccgttaaaa gagcaaatga
    18061 cgattcgcat cgatggattt gaaaatgtgg cactgtgggg gcattttcag cccaatttat
    18121 ataagcttga attggagacg atagacgaag aaaaaagagt aacggagtat attgactatt
    18181 ccttcggttt tcgtaaaatc gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga
    18241 tgattatcaa cggggtaaat cgacatgagt ggcatccgaa aaaaggacgc gccatcacta
    18301 tagaagatat ggaaaaagat ttggctatca taaaaaatgc aaagatcaac ggcgtacgga
    18361 catctcatta ccccaaccag atcacttggt attatttatg cgaccaagca ggaatccatg
    18421 tgatggcgga aacgaacctc gaatcccatg gctcatggca aaaaatgggc gcatttgagc
    18481 cttcttacaa cgtaccaggt gcagtacctc agtggcaggc ggctgttctt gatcgagcaa
    18541 aaacgaattt cgaaacatta aaaaaccatc caagtattct tttttggtct cttggaaatg
    18601 aatcttatgc ggaagaaaac attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg
    18661 atcgacttgt ccattatgaa gggaattttc caaatccagc attcgaagat tcgatttcag
    18721 atgtaaaaag ctgcatgtat gcgccaccag aagagatcat tgactacctt gaaaataaag
    18781 cggaaaaacc gttcatctta tgtgaatata tgcatgacat ggggaattcg ttaggtggaa
    18841 tggattctta catgacgttg cttgatcgtt atgaaatgta ccaaggaggc tatatatggg
    18901 attttaaaga tcaggcgatc caagtgactg atccaatcac aggcagagaa gtgatgcgct
    18961 atggcggtga ctttgacgat aaaccaagtg actatgaatt ctcagggaat gggatcgttt
    19021 ttgcagatgg acaagaaaaa cccgccatgc aggaggtaag atattattat gaaaaataca
    19081 gtaaataaaa gtcatatgga tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta
    19141 ggtgtcaaat cggggaatac gcattatatt ttttcttata caagaggcgg actggaatcg
    19201 ctcaataaaa acggaaaaga atggctatac cgggaaacac ttccagtttt ttggcgagca
    19261 acgaccgata atgacagagg tagcggattt ggatttaaat ccggtatgtg gttaggagcc
    19321 ggattgtatc caaaagtcaa agaaatagaa gtactggtgg acaataaaaa gatcgatttg
    19381 ccgattgctc cagtaaataa tcaatattca agtaatgaat acgctgaaac ggttgaaatc
    19441 atttttacac tggaaatcaa cacagtacca aaaacccaag tgacaatttc ctaccaaatc
    19501 gctggagagg gtaaaatgct tgtcaatatg cattattatg gacaaaaagg attaccggaa
    19561 ttacccgcat tgggtttaag gttcattatg ccaacgaaag caaaattttt tgagtatgaa
    19621 ggcctatcag gggaaacata tccggatcgg atggctggtg gtgtcgaagg tatttataaa
    19681 gtggcaggac tccccgtttc cccatacctt gttccccaag aatgcggcat gcatatggaa
    19741 acgaaatggg tagaactaac aagaaatcaa acgttggata atacggataa agatcaagaa
    19801 gatttttcgt tgcgatttga acagcaggat acgccatttg ctttttccgc tttaccattc
    19861 aaagcagaag aactggaaca ggcaacacat atagaagaac taccgccaga aagacgaacg
    19921 gttcttacga tttttgcaaa agtaagagga gtcgggggta tcaatagctg gggagcggat
    19981 gttgagtcac cttaccgaat cagcagcgaa gaggaccatg aaatcggctt tattattcag
    20041 tagcaatctt tagattatta tttgaaaaac ggagtttaca agacaaagct gctttattct
    20101 atatgtcatg aacaagagag taaaaaagtt ctcagatact tttgttcatg actattttct
    20161 tcaataaaac caattgttat ttttctgaaa aacaaagtta actatcagaa aattaaaaaa
    20221 agtttcataa aaatgattga cagtcgattt
//
LOCUS       DBKOIJ010000043        19053 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000043 DBKOIJ010000000
VERSION     DBKOIJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.043"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(34..1113)
                     /locus_tag="ACV5NJ_001174"
     CDS             complement(34..1113)
                     /locus_tag="ACV5NJ_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HHY6485276.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            complement(1103..1960)
                     /locus_tag="ACV5NJ_001175"
     CDS             complement(1103..1960)
                     /locus_tag="ACV5NJ_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HHY6485277.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNVNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            complement(2035..3870)
                     /locus_tag="ACV5NJ_001176"
     CDS             complement(2035..3870)
                     /locus_tag="ACV5NJ_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HHY6485278.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(3919..6309)
                     /locus_tag="ACV5NJ_001177"
     CDS             complement(3919..6309)
                     /locus_tag="ACV5NJ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HHY6485279.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(6577..7824)
                     /locus_tag="ACV5NJ_001178"
     CDS             complement(6577..7824)
                     /locus_tag="ACV5NJ_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HHY6485280.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            8217..8633
                     /locus_tag="ACV5NJ_001179"
     CDS             8217..8633
                     /locus_tag="ACV5NJ_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485281.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(8650..9387)
                     /locus_tag="ACV5NJ_001180"
     CDS             complement(8650..9387)
                     /locus_tag="ACV5NJ_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485282.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(9809..10372)
                     /locus_tag="ACV5NJ_001181"
     CDS             complement(9809..10372)
                     /locus_tag="ACV5NJ_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485283.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(10359..10886)
                     /locus_tag="ACV5NJ_001182"
     CDS             complement(10359..10886)
                     /locus_tag="ACV5NJ_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485284.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(10909..12282)
                     /locus_tag="ACV5NJ_001183"
     CDS             complement(10909..12282)
                     /locus_tag="ACV5NJ_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HHY6485285.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            12366..12845
                     /locus_tag="ACV5NJ_001184"
     CDS             12366..12845
                     /locus_tag="ACV5NJ_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485286.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(13049..13192)
                     /locus_tag="ACV5NJ_001185"
     CDS             complement(13049..13192)
                     /locus_tag="ACV5NJ_001185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485287.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(13195..13512)
                     /locus_tag="ACV5NJ_001186"
     CDS             complement(13195..13512)
                     /locus_tag="ACV5NJ_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485288.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(13623..13805)
                     /locus_tag="ACV5NJ_001187"
     CDS             complement(13623..13805)
                     /locus_tag="ACV5NJ_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485289.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(13842..14642)
                     /locus_tag="ACV5NJ_001188"
     CDS             complement(13842..14642)
                     /locus_tag="ACV5NJ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485290.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(14779..15063)
                     /locus_tag="ACV5NJ_001189"
     CDS             complement(14779..15063)
                     /locus_tag="ACV5NJ_001189"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485291.1"
                     /translation="MTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMSRFLKEREK
                     KELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ"
     gene            complement(15194..15502)
                     /locus_tag="ACV5NJ_001190"
     CDS             complement(15194..15502)
                     /locus_tag="ACV5NJ_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485292.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            complement(15664..16194)
                     /locus_tag="ACV5NJ_001191"
     CDS             complement(15664..16194)
                     /locus_tag="ACV5NJ_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485293.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            complement(16210..16575)
                     /locus_tag="ACV5NJ_001192"
     CDS             complement(16210..16575)
                     /locus_tag="ACV5NJ_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485294.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            complement(16581..17324)
                     /locus_tag="ACV5NJ_001193"
     CDS             complement(16581..17324)
                     /locus_tag="ACV5NJ_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485295.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            complement(17455..18177)
                     /locus_tag="ACV5NJ_001194"
     CDS             complement(17455..18177)
                     /locus_tag="ACV5NJ_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485296.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            complement(18183..18428)
                     /locus_tag="ACV5NJ_001195"
     CDS             complement(18183..18428)
                     /locus_tag="ACV5NJ_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485297.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            complement(18439..19023)
                     /locus_tag="ACV5NJ_001196"
     CDS             complement(18439..19023)
                     /locus_tag="ACV5NJ_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485298.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
BASE COUNT         5456 a         3806 c         2677 g         7114 t
ORIGIN      
        1 tttactgtac tctatcaatt tttataatct tcattatgct tttcgaaact ctttgattaa
       61 aagaataaga acctttagac ttgctaatgc tcctattaca gtccaaatga ctaaaagcat
      121 tttctgatgt ttattggtaa ttcctgtgaa tattgaactt gccttttcag caaatgttct
      181 gtcttgcatc tctacgctac cttgttccgg aatattaaga tcattatttc catctaattc
      241 cttgttggca cgatcaacat cctcaaataa atcattagaa gatgtaacag tttctgtctt
      301 atctgaaacg gactcgctat caatggcatc agcgtatgcg gtattgccaa ctgttacttg
      361 tggcgtgatg tattttgctt tttcatctct tagacttcta actaataaac gatcgtagtt
      421 ctgaggatat ggatgacaag tataaagtgt taacatatct aaatcaggaa tatcagtcag
      481 tgttgaattc tgaccgtaat ttctaataac gtctgttcgg aaaaccgtat aaactgcttt
      541 ttcaccaaga taatctatat aaatgtggtc tccggctact aattgatcaa cataaagaaa
      601 ttgtggtgac gtataaccgc cacgatgacc tgctattact gtttgatgac ctttacctga
      661 taaaggtaga ctagtagcac tcatctgtgc cacacctcta tataaatgtt cctgtgaagc
      721 gcctagaaac aaaagctcta gcacatttat ttttggaata tagacataac caaccgcccc
      781 tgaataggta gatggttcaa aatctttcgt ttgatttttt gaaaaaacat ctcctgtgta
      841 aggattgacc tttccttcag cttcaccaat atcaaatgat ccgccgtctg ttttctgagt
      901 attgtctaat ccttggttac cgctgttcga ttttatttgt tcgacttttt ttgccacttc
      961 ctcttttgaa ggagtttgtt ttttctcaat tttctcttgg atttccgcca cattctcttt
     1021 tgttttgaat tgatgataga gctgatttgc aggataaatt gttaaaacca aagtgcaaat
     1081 aagagtcaaa aaaagtagta ttttattctt catttttcat tcctcgtctc tttgctacta
     1141 tttttttaat aatgactatc acagctatta ttaataaaac ccctacaact acgataggat
     1201 accagttcat ctgcgccttt acctttaaat cttctttctt cttttctttc tcaacaatac
     1261 ttttttggta aggtatgcta acccctctaa cgagtaacct atgagaatta tctccaacag
     1321 gtgtacatgt cataagtgtt acataatctt ttcctgccac aggaagcaaa tcctcaaatt
     1381 cggttggtag gattgttttt atccgatcta cttgataagc aagctttttc cctgaaatat
     1441 caataaaaaa tttatcacca agtttgagca actctaaatc tgtaaaatat tttttattag
     1501 gaatgcccga atgagctgat ataacagaat ggtttccaat cccaccagtt ggataatcag
     1561 ttccggttaa tattcctgct cctgctgata gttgaaaatc gtttacacca tcataaattg
     1621 gcaaatccac attaatcttt ggtataagaa tttcacccac taattcacct aatttatcct
     1681 tctgaagatt tacatttgac ggaatattgt tattatcttc aggaatcgtg actgaggttt
     1741 gttctgtgtc ttttcgtttc ctattaactt ctgaatatct cttgaagact tcttcattct
     1801 ccttttcaga agcttcatca tatttttcct tataggagtt tagtgtttgt tgagtttggt
     1861 agtaggtata agcctgagta attacaggat atagacctac tcctaaacat agcaacatcc
     1921 caatttttaa gatgatcaac ttgatattct tctttttcat gctcatacct acttttcaca
     1981 cactttttaa aaagaaaccc tagagccaaa aacactctag ggaattttat ttcattaagc
     2041 tttgtgttgt tctcgtttgt agacaacata tagccctgtt cctgccacac ctgcaaccac
     2101 tagaaatact actgttccaa ttccacctgt acgaactgtt tcaggtgttt taaggttctt
     2161 gatgtctgtt acttcgctag aagcatcatc attcgatacc acaaatggaa catcatttct
     2221 agttggtgta tatcctgaag gagcaacaac ttcttcaaga gcataatcat tgattttttt
     2281 atctgtgatt gttggattac cttgtttatc aaaatctaca tcataggtat attgcaagcc
     2341 ttcaatttgg aacttaccgt ctgatccgga aacgaattca gtagctttag atttatctgc
     2401 aatccaagta acagctttcg ttgttggatc ttgttttagg taagtaactt tattcggatc
     2461 aattttgttc ttcactacaa attttgctcc actcaatgct ttgcttgtat ctccatctgt
     2521 tttaatgaat tggtgatcac caatgattgt ataagcgtga tcaactggtg tttcgtcttc
     2581 gggttcatat ccattgttgt agatcgttga aacatcattc ttaagtggtg tatccatttt
     2641 agctttacta ttagtatgag cgttcatcaa aattgtaaag aagcgatccc ctgcatcaaa
     2701 ttgtttctga agcttatcca tacctgattc agtaaagaca tagtttgtcg tttgtccgtc
     2761 acgttcgata ctgtaatcag tatctaaagt cattccttcg atcacttctg atgaatcata
     2821 agttaactct gcacctagaa catctttgac aacatatttt tcgtaattac caatattacc
     2881 aggaattgat ggtttcaatg tatattgaac atcttcgcca gtatcaacgg tttctttatc
     2941 aattacctta gtaattacag gtcgtttgta gttggtaaca tcaagaacta ctttagcagt
     3001 ctccccacgt ttaacgctaa atttgaattt tttaccatct agtaaatagg ttgttttatt
     3061 agcatctgtt cccaagtcgg tttctaggaa ataatagtca cctaattcca aaccatctac
     3121 agttaattta cctgtgctgt ctgttttata agttccaaca acctgatcag atccatcatc
     3181 ttttcgcaat tcaaaaacag ttccggcaag agcctttcca tcttcgtctt ttttatcaag
     3241 ctctgctgag tttaattgga aatagttttt aggatagaca tgtacatcat aattaaagta
     3301 ttgatcatct gatccgttta gttcaggtac gttaacaatc ataggatacg cttcttcaac
     3361 tacacctttg actgttcctg tttcaacaat caaatacaag ttatctgtta aatcgtctgt
     3421 atttacttgt ataggtatgt ctatattccc ttgtgaatca gtcttcattc tacttacttc
     3481 actgtatttt ttgtttagta ataatcgtgc tttagcatca tcaatatgtt gaccttttgt
     3541 agtatcacta tccgtccatc ccaaggattt agcttcatct ttagtaagtt tcacataaga
     3601 gaactcaacg ccttcaagag gttttacacc atttggtaaa gctatttctt tccctttgtt
     3661 atcaatatct gagacagggt tcccatcccc attagtattt gttactttat ggatatgaat
     3721 cgttccagtt ccttgataat tatagttttg ttgtgcatat gctacttgcc cccctagtcc
     3781 aaggctagaa gagattgagc ttgcgcccaa tcccactcct agcaaagtac caaatagcat
     3841 taaatttgaa aaaatttttt ttgatttcat acgtcagcct tcttttttca tatttacttc
     3901 ttttccatta acactttttt attttgcttt gtgctgttct cgataataca gtagcaccat
     3961 actcaatcct gcaattagaa atacaagaag gaaggctgat gtaccgattc cgcctgttct
     4021 tactgtttca ggtacaaggt gattaattac tgtatattca aaggtttgtc catcgtcagc
     4081 aacggtgatg ccttctttag accatttccc tgtataacca ttagggtttt gtgtttcttc
     4141 gattttgtac gttttgttgg caacaatatt aaatgttgcc aagccatttc tgtccgtttt
     4201 ttgtacaact tttacacctt tgtcgtccgt caaagtgaat tctgcgtttg ctaatgcttt
     4261 cccttgatca tcgactttat gaactttgat cacacctttt ttcaaagtgt ttttagctgt
     4321 atattcaaag acttgtccat catctttgac ggtaattcct tcttgtttga atgcgcctgt
     4381 gtaacctgtt ggtacttttg tttcttctaa gttgtaagtt ttgttcgcaa taatgttgaa
     4441 cgtagccaga ccagttgcat ccgtcttttt aacttctttt acaccttttt catccgttaa
     4501 ggtgaattct gcatctgcta aaggttttcc atcttgatcg actttatgaa cttttactac
     4561 accttttttc aaattatttg tagctgtata ttcaaaagtt tgcccatcgt ctttaacggt
     4621 gataccctct tgcttaaatg cacctgtgta acctactggt actccggttt cttctaggct
     4681 atacgtcttg tttgctacaa tattgaagct tgcaagtcca ttttcatctg ttttttctac
     4741 ttgtgttgca cctttttcat cggttaaagt gaactctgca tttgccaaag gttttccatc
     4801 ttgatcgact ttatgaactt ttactaaacc ttttttcaaa gtgtttgtag ctgtatattc
     4861 aaaagtttgt ccatcatctt taacggtaat tccttcttgc ttgaatacgc ctgtataacc
     4921 agttggtact ccggtctctt caaggctgta ggtcttgttt gctacaatat cgaagcttgc
     4981 aagtccgttt tcatctgttt tttctgcttg tgtttgggat gaatcatccg tcaaggtaaa
     5041 ttcagcatta gctaatggtt ttccatcttg gtcaaccttt ttaactttta ctttaccttt
     5101 tttaattgtg ttcgtagcag tgtaactaaa ttcttgtcca tcggtattaa cagtaatacc
     5161 atccttttta aatgaacctt gataaccttc aggaactttt gtttcttcta agctgtatgt
     5221 taagccttgt tgcaaatcaa acgtagctaa cccatcatct ccggttgtct taacagattt
     5281 agcattgtta ttgtcagtta gagtaaactc cgctcctttt aaagcatttc catcttgatc
     5341 aactttctta actgttacct gaccaacagc tcttgtccat ttagcactca aagatttaga
     5401 gtcctttgtt tctgaagtag tgatcataac tacggcatct tgatacacac ctcgtccatc
     5461 aaaagtaaaa ggattggttg tgctaacatt agcagtacct ttaaatgaca aagtaccact
     5521 tggtgtagtc acaggtactt taatagtaaa acttgaattt aatttcacag tgttgttagt
     5581 aatttcttta ccatctgcat cataaatctt cataccagct ggttttgctg atgtaaaagt
     5641 gagcttcgca ttaccagtct tatttgatgc tgttttatat gtgaaagtgt gatattttcc
     5701 ttcgtcagct gtcttagaag ctgtgataga aaactcagtg ttgggcgtat cttttccatt
     5761 cttagcatag tcataaattt tttcaaatgc tttttttaca cgagctgttt ctgcggcggt
     5821 atggtttgga tgtgaccata caatctgtga aactttaaag tttccggcta aaacccatga
     5881 aactaattgt gttgcatacc atgcttctgt atcatttcct gcacccagtt cctttgctgt
     5941 ttttgatgga aaaccacctt taaacatacg atagaactca tcactgccag ctcccttgta
     6001 cttcaagctt ccggctccac ttcctgttgg ggatggcaat tctgaatcca tacaatatcc
     6061 aatatttcca tgagaatctt taaattgact tgaaatcatc cattcatttg gatgattcgg
     6121 catattaacc ctaatataac cgtctgtttt tacgccagtt tctataaatt ttgcacttgt
     6181 cggttttgtg acatctgctt cagctcttat tgctgaaaaa ggcattataa gcgtagataa
     6241 catcattact aaagtcgcaa aacaataaat tatttttgat tttaaaatta tcgttttttc
     6301 atgcttcatc tctcgttact ccttctttat accagttagg cgaagaaaat gagccacgat
     6361 atgtcggtgt ggtaatgacc ttcgcgccca ttcctaagtt aaaatcacca cacctattaa
     6421 tcattttttt cgttgaaagc aagctgtttc aacgctctag tattcatagc atcacccctt
     6481 ttagttctac ccaccatgag caacaagttt tctaagtagt taagcaagga caaataaaga
     6541 ctatataact acttagtata taaacgatat cttttattaa cgtaccgaaa ccgctaattg
     6601 agatacgtta tatgctgttt caaatgatcc tgcattaatc caacttgccc caaacacagg
     6661 atcgacaaca tggtaaaaac cgtctttaaa tccatcaacg gctactacat gtcgattcca
     6721 aatagcatcg ccccaaaaca ttggtttatg ttgagcaggt tcaaaggcat aagttaccca
     6781 cgctactaca ggatttccat ttacaatctc atcttttaat ccttgtaccg atgaaccaga
     6841 aatatctaca acatttccgc cattagcaac accccatgaa actacaggag ccggattcat
     6901 accttgataa gttccatcca cattgtgtct ccattcacca gaaaaaccat tatttggatt
     6961 attgtcagga gatagtggca ttgatttaac aaaagtcatt aaatcttgat ttgttgcaac
     7021 acctttataa tgtaacgctt ctagcaaaga tgttccttcg caaagcattg tgttaccttg
     7081 actaataaat ggaacgccta gcacaacatt agttggttgt tcgggtttac ttggtgaagt
     7141 agaactgtca gtttcatcac tcgaacctgt ttgtgattga tcactagaag atgtactggt
     7201 tgattcattt gcatcatttg atgttgattg tgagttattt gttgatgttc cagtatcagc
     7261 ttccgtagtg ctactttcac ttgttccctg tgatgcttct ttctcacttg tagaattatt
     7321 tgtattagat gaacttgctc catcaacact tgaagcactt gtttctgtct tatcagaagt
     7381 gtttgctgtt ggttccgatt cttttgtagt ctcggatttt ttgttctctt gctccgtatt
     7441 ttcaatagga tcaggtttat ctttttgaac agtttgacta actacttcgc catctgcttc
     7501 cttaactgtt aatgtatctt tactgatgtt gaaagttcgt ccaatcggga tagtccgttg
     7561 ttctcctttt gccaaatcaa cattgttcaa ttcggcaaac tcagcaacag tagtcttgta
     7621 acctaagctt ttaagcttct ctacagttga ccaaatagta tctccttttt tgaacgcata
     7681 acttgattgt cctgttgtaa tttggatttg atccggtgtg tttgcagtcc attcatcagc
     7741 atgtgaggtt actggtactg ataaccccgc aagtacagct gatgccaata aaaccatacc
     7801 aagcgaattt ctcttcatat tcattccttc tttttgtttt tattttctgt ttgtgatcgc
     7861 aatgcttcta ttccttcttc cgttataata taggttttgt cacccacttt cttgaacgaa
     7921 cctttaggca aacgatttgg ataatagttg attatgtttg taacataatt tctaccttta
     7981 cccaaaatca aacttgcttc ccttgcggta tacaagttgt atttgtctag gttcaatttc
     8041 tttttgtttg cgatttagac ctaccccttt cgcaaaattt attgtataat aatagtacca
     8101 ttgcactgtc ataattgcaa tatttttttg gggtttatct tcatttaata gcctaaaaag
     8161 gcttgttaga taaggaatat ctaactaatc aaaacaaaaa aggatgttaa gaaaaaatga
     8221 acaaaaaaag gaaattatca gtattaatta cacttgtttc tatattgact attcttagcg
     8281 ttatcttgtc gctgaaaata ttcaatcaaa taaacacaag ctcaaatgcc aaagatggaa
     8341 tagtcgtaga aaatacctct aattctaaag aaactggtac tactacaaaa aaagacaaat
     8401 ctgctgagat cactacgaaa aattatttaa taaagactgt ttattttgat gaacgaaaaa
     8461 cagcaattct caaagatgag gaaggatcag aattaaagct acctataacg gtatccgata
     8521 agttcaatgg aacgttctcc ttacaaaccg ctgaaggatc acctgacaat ctcaaagttg
     8581 ggcaaacaat cattcttggt aaaaacgacg aactgtattc atttgtaaaa taaaaaaatg
     8641 gagcaaaggc tagtcaagcc cttgctccat tttccattct tcaagaggtg tagctatttc
     8701 aacaccaaag tttaaatact gtggatgctg tagatacatg atatccattc tattttcgat
     8761 aaactctttt aacgttgagt agatgtattt ttctaatgct atatctgccc aactaccata
     8821 atcaagttta ttcgtgatct tgacgtttat ttcaatttga gaaacatcaa tattttgaac
     8881 atcaggaaac ttattcagtc ttagctcttc tctaaccgct tctactagcg gttcatattg
     8941 tccgcctagt tcgctaattg ttgaaactat ttcatctgaa ttccaacgat cttccaatac
     9001 ctttgctaca ttttctttag gcggttgtct ttctataaat tcttcaggtg tttcatcaaa
     9061 ctctactcta aagttcaatt tattttgatt gttagcatac atataatcat ctctgtttga
     9121 ttcaaaaaag ttacgaattc ctgtgtctga tttatcactt aactccttat ttgctaaagt
     9181 agcccaaaaa tcccaagttc ctttattttc agtatacaaa gtaaaattaa tgtttatata
     9241 gtttgcagtt tcaggatacc cttgctctct aagagcatct ttaacaatca ctgctaagtc
     9301 agtttcaaaa ttctccataa aaaattcact aaattctttt ggattccacg tttcttctaa
     9361 taacttttct aatttatgat ctgccattat tattctcccc cactttactt tcaatcttat
     9421 atctaaaact gcttaaaatt aagagctata ccttatcata aaaggtattt tatgctttat
     9481 ttaagtctac aattctatat tacaattatc atctagacca ttttcttatg tcaaataaac
     9541 catatatatt gtctttaata gttttatttg actttttaag aaagtattta cttttgactt
     9601 tttttacgtt ttacaacata tacgtattgt aatgttcagc tcaatttatt ataatctaaa
     9661 tgaattgatt aataaaaatt ctgaggttcg ggttttttat caattctttt ctgtttgtgt
     9721 ttgtgatgaa acaatccttt ttcggtaggt tttgttatca tcgtcagcac aaagagctaa
     9781 cgttggtacc gttagctctt tttttatttt actctgctct tacatagcta tctaaatcct
     9841 ccaaataaaa caattgattt tctcctatgc ttgttactcc acgccaaatt gttgttgttg
     9901 ctgttgttgg agtatttctt gtctgtgtaa tcaattcaac cattttacct ttttgatttg
     9961 ttgtatagta atcagcagta tagctagata ttggactatt accagtgcct accatcgtgc
    10021 cagaaatatc aacaggacta gactttacta tctgaggaaa ttctttttgt atttccttca
    10081 tattcttcgt atatttttcc catgaattcc agttgtaaaa taaattaaag aatttcctac
    10141 tttgttcctt aatttcagca tttcccaatt ctttgtattg agcttcctgc atagctttca
    10201 ctttctggtt attcttgact acttcgattt gtttactttt aaggtcttcc aattctttga
    10261 cattattatc ataagcattt ttcttttgaa atagtaccac acaacaaagg atcacaatta
    10321 tggaaagtat tccgatcgcc tttttagcag tagtgatttc atgattttgc ataaaaagca
    10381 cccccagtca ctttcttatc attatttagt gtgatggtta atgtggtatc atacactttt
    10441 tttccattgt attgaacttc aaaatcggtc aatgcttcat acaacccact actagatgtc
    10501 ttaattccaa agtttgttag cttaaaagta aaaggcttat agtactcgat tttttctttc
    10561 tcactactct tatcagtaat tggtggttta cctgttacaa taaatagttt tctcaaaaat
    10621 tcttcacctg attgatctgc aaacttattt tgaactgatc cattcaacat ttcttgaata
    10681 tcattttcta aatctttccg taatccatcc acctcatttg gtttaacctc tttaaagaat
    10741 gtcagcttat cttcaatatg cacactgtgt ttttcagctt ctatgtcctg attctgctta
    10801 ttgaagaata ggattccaac aagcagtgct aataacatac cagaaagcaa agaaatgata
    10861 attgtatctt tcactttact attcatctat atcgtccttc attccaattt atttttcgat
    10921 ttcaaattta gcttttccta caccgctaaa gaaatgcaca ttatctgatc cgtccactcc
    10981 atcaatcaac gttaccgaaa ctccccttgt tggagcttgg gttggtggaa cattaaagtt
    11041 agcacataga aatgatccat cttcaaacac cgctataact acaccagtat gccctgcatc
    11101 tccaacagcc cataaccatc catccgaagc actaaaccca taacctacga ttggtttgtt
    11161 cgttattttt gctcctgccg atttgtagct ttcccataca taaccaccat ttccagtagt
    11221 cggttgattc ccatcgtata attggctcat ataagcccac gtcaactctg tacactgacc
    11281 ttgatatatt ccgttaaaag gtgaagttga ccaatctggt tctttgaatt ttggtagcgt
    11341 gatcgctttc ccatattttt tcaaagcttc ttcataagtc atattcgcta ttacttgacc
    11401 ttttttaccg ttaatttttc cttctttccc aattgatcca gaaccagaat tagcgtcatc
    11461 cgtagttgca caggcatctt ctactgttcc tgatgctgaa cttgatgaat tattcgatcc
    11521 atctccggta cttgaacctc cggatatttt attgatctta ctttcatctt ttgcaccgtc
    11581 catacccttg cttttcatgt acttccagat ttcttttgca ttttcaccac gtacagctat
    11641 tacagctgat tcggaatcag ctgaaatttc ccagttttta tgaaaagtta ctgcattttc
    11701 tacgacatca tcagaagcat ttaaagcata atcttttaat attcctacga atcccccatc
    11761 acctttgaac ataaagtcca attgggcttc tgtagtccac caagaaccgc cattattttt
    11821 cttagaccaa tcaactaatg ctgtatgtcg cccagctgtc cattgaccat agccaatacc
    11881 catcagacca tcagtaaatt gtttgatgaa gtcaacttct ctctgtccat ctccgtaata
    11941 cccttctact gctcgaggat caatacctga ttcttgtttc cagttaccta aaatacctgc
    12001 aatactagca cctgataagc catattctcc attcatgtat ttgtaaatat cttcaatatt
    12061 tttggctaca tcacctgtta cagcgccact tccagtatcg tttccatcgc tatctgttcc
    12121 tgacccacta ccttcagtag tacagttaaa ttgtgcatca taagtagcta tcccatcagc
    12181 atgtatcact tgcggattta ttgtgattac actcattgca atccctgtaa attgcaccat
    12241 taacataagc aatgtggcaa tctttctctt agattttttc aataactacg cccccaaaaa
    12301 gtgtactttt ttatgttatt atgataatat aataacataa attaatagaa aggaaaagct
    12361 aataaatgac tgataaaaca agtgatttac ctaaactagc tccaaaaaca tctcgagtaa
    12421 agagcaaaaa agatggaaaa acaaaaaatg ctgtaaagaa aaagtcagta aaagaaacat
    12481 cctcgttact acttgatatt ccaatgagtt cttttgatga caatacggac gaaacttctg
    12541 ttaaaattgc aactcacgtt ggaatgaaca atcaaaatga ttctttttac aacttgttaa
    12601 acagcctaag agagttggaa gataaaaaaa atcaggtaat cgcatcaaaa gctcctgatc
    12661 tcacaaccga tttggatgct gaaattatcc aaatcaaaca agatgagcga tcagttaaat
    12721 cgctactgaa aacctacttt gatggttttg ataaagaact agctccttat agccaagcaa
    12781 tcctaactaa accaatacaa gctccacata tttcaatttc cctacaaaaa aagagtgatg
    12841 attaaacaat cattactctt ttttagattt ctggtaagcg ctgatcgcaa aaaccatcaa
    12901 caatattgat tcaaaaaata tgttcattcg tgtctctttt ttccattata gatttcccac
    12961 tattccatta aatcgaaagt cacaccgatt actacaaaga ttaacgtgac cacattcaaa
    13021 aacattaaaa gatatgctga taatctcatc atttttttag ttcacttact ggtaatctaa
    13081 acatttgaac gattgcactg gaaataccat aattagcaat gacaaacaaa aaatacgcaa
    13141 taatttcact tacacctaga tagatcagta aaataaataa gtcacttaac attgttagtt
    13201 tcctcccagt atatgatttt catttttggg aagtctttta taatccaatt cctcaaaaaa
    13261 aggaaccata gtgtcactaa tataggtttt agtttcaggt accgccaaaa ttccccaatc
    13321 aagaatttct tcccatgata catcatcaaa ttctaaaata ttatgatcaa ctgacctatt
    13381 accaagttca tatccaccaa agatcaccat attatcaatc atgtatagaa tttcatcagg
    13441 cgaaccatga gttaatgttt cgtcctcttc aagcagttcg tttaatcttt gtttgtattc
    13501 ctctcttttc atacttttat cccttctgac tcattttttt gaattttaac atttttaacc
    13561 caagcatgac tatctttaca tgctattaat ttttatctcc attttgatat ttctgttttt
    13621 ttctactcac caatcaaccg taaatcctca atattctcac cttcaagcga atatgaatat
    13681 acttcgccgt aaatattttc tataactacg acttcttctt caaagtctat ggactgcaca
    13741 tcaaaccatt ctcctccgcc aaattcgtct atgattagta cctgcaatat tttatttact
    13801 gccatttcaa tttcccctta taccattcta tgtcttccaa actatttttc ttctttccat
    13861 accaaggttt cacgtatctc aaaattttct ttaaataagt gttccggtac ttttataatt
    13921 aactcttttc ctacttttat cagatagtct ttttcaccta atgagatttg accagtcggt
    13981 aactgtatag aaactctttt agttttatta aattttttag ttttcaatac agtatacggt
    14041 ctacagaatt tctctaagcg ttcaaatctt ttactatcgt attggaaata agctttagga
    14101 acagataaag tacaaaaact atcggtaaca gcacttatgt cttttttaga aattatccca
    14161 actttagaac ttctaaggaa agttgctaat ttcttatatc caccatgact tgtttcaata
    14221 tagatgctat tgtattggct gtcgtctatc tcatttattg tttcaacctt tttcctgaca
    14281 ggtgtttcaa cgagttctcg aacctcttct tcactgtacg ctaatacaat taactcttcc
    14341 ccatttacca tctttagaac atacgcttgc ttcttttttc ttggcttatt ttgagattca
    14401 acataaccag acatggaatc tttatacttc caagcatgta cttttttctc taatttctgt
    14461 atctcttttt tcagctgtag atattcagac attttttcat tgtaacgttc gcaatataaa
    14521 caatattcat cttttccaaa atcatgatca agtccttctt caatatgttt attcaacaaa
    14581 ccggaaaaat actgatcata ctgcttcttt cgtaacttca gctctcgtaa atcttcagac
    14641 atttcatttt tcctttccaa ttattggtgc catttcacaa tttgggattg actaaacacc
    14701 ataattaatt tgtaagctaa cacaattaca actgttggta attgtgttag tctccatcaa
    14761 cttgtctggt ggcttttttt attgcacaaa tttcatagct ctcaaaaagc gcttggcttg
    14821 aaaaggtgtt attctacgaa ccttattcgc caatttccaa tctgataaag caatttgcca
    14881 ttcgtgcatt tcaaaatccg gcatgatttc tttgatatac tgtagctctt tcttttctct
    14941 ttcttttaag aaacggctca tcgagtttaa cattggaact aaggatgcgt aggtacttga
    15001 ataacttcca atgcgtttcg tagtaattac ataatcaata aaaacactat taagaaaggt
    15061 caagtttata aactctgatt catacactct aaaatttgca gttaatcgct tcgccttgtc
    15121 accccaatag tcacttttgt tgtaggcttt ttcccctcga atatagcact ctgtttctct
    15181 ttcgtaataa tagtcatact tttttcatca tcaaatctga gagtttttga ttatcttttt
    15241 cactgtaaaa taatctactc gttaaacttt taggataatc tttgatagaa tatgcatccc
    15301 aaacaattct ttgaacatat tcacgatcta tattttctct gacttcctca ctccaataat
    15361 gttgaccgta atagcatttt tctattacgt tgttagcaga aacttcaaaa taggattgtt
    15421 ccctttgtat cctcatacca atcatcatag cgcaggaaat aactattaat aacaatcctg
    15481 tttttaatct attattccac atgcagtttc cacatcctta tcttgataaa aatcctcttg
    15541 accctctcca attactgttt taaatataac caccaaaaca agatcatgct aagaaatcca
    15601 agagtatata agatgataca ttctttgtta attatccaaa aaactctgat ttctttgagc
    15661 ttttcacgca ttaggaaaca ccttgttata tcttttttgt atttctttca attgtttttt
    15721 aaaactcata aacaactgtt catgatctgg tctaagcgtt agaatatcta aattatttat
    15781 cttttttaat tgtgtaataa aatctatttt ggattttctt tcaatatcaa aaccgacaat
    15841 aatctttcct gttggatcaa cgcatttcac ttgaataact ttgtcatttt gcgatagctc
    15901 taatattgat gatttttctt ttgcctcttc caaaagatgt tctaacactt cgttaggtgt
    15961 tccatgatag taataaatca tggtcttctc gaaaattttt atcggatcaa caccatattc
    16021 ttttccgatt cgataaaaaa atttcattgg aatttcttcc ggatttctgt tcctgcttga
    16081 ccagttagag atggtttgat aatttgcaaa atggattcgt tccgctaatc tctgaactgt
    16141 ggtctctaaa tcttcttcta ttaattgttt tacagcacta atgttttgtg acatttgctt
    16201 tctatccctt caaattcgtt gcattatcaa ttctaagatt tcatgtttta gtgcatttct
    16261 atctacattt tgctttttta actctgcacc ataatatctt cgcaatccct tatccaagca
    16321 tacaattcct tgatctaata gatcatattc cgctttttca aaccttcgct ttgcctgctc
    16381 aacaagcaaa atgatattgt gatgtatctg attaaactgc cgatcatatt cctctaaagt
    16441 tttagcatct aagttagcaa tatgatcctc tgctaatttt tgtgttaaaa ataattcgac
    16501 tttctcagat ttcaacatct gtattttttc atactcttgt tttgcttgga tatagtcatc
    16561 tttcaagcta gacatctaac tcatctcctt gcctgtataa actgatacgg actgtccaaa
    16621 attgaatagg ttggtgatga cttcaagaag acttcacctt tttcacatga aaataaacca
    16681 attaattctt caatttcatt tacgatcaga tatttattag ttaaaatttt cccattttct
    16741 ttttctagct cgacaatcgc ttgattccat gtattttgca ctaaatcttg aatcaatttc
    16801 aatctatcca cttttttcat ctcatacagg gcaatttcag cttgtttaat gccttttaat
    16861 ttgcgaaagg catatttgta ttgtgtgtag gttaaaaagc ctgtgtagtt atacatgggc
    16921 tttcctgatg tcttgtagtc atttaagctt ggatatattt cagttttcca tttttcttct
    16981 gaacgttgat caataattct taatttttca ggtaaatcaa tttcatagta ttcttcaatt
    17041 ttcagctcac gataaagtag atgtttttcc ttttcttttt caattacaaa agaattattt
    17101 cccttcagtt tagctatttc ttcacttgtg agaccaagcc taattttttc tacttcactt
    17161 ctaagttcac gaatacttgt tgcctttaac cacttcccat ttgcaccatt attgtaaccg
    17221 gtaagtaact gtattttata aattccttca ccatgaagca ccttatatat accaacctga
    17281 ttcttttgcg gaatatgagc ttcatttgca ctgatttctt tcattacgca ttctccttta
    17341 taatctagtt aattagcttg tgtgtttcga gtagttgagt aagaacgttt gtttcagaaa
    17401 tcatttattt gttatcactt aattttaaaa aaatagggct tacgccctaa agatctattt
    17461 aactttggat tgatcgtcca taacaggctc tgattgttga tataatttat atgctcccaa
    17521 atccatataa tatttagttg tactttcaat catttcatca gttgtatttg ttgtcattcc
    17581 cattacatcc cagtatagtg tgccgttaac tttctttggt gttactactg caactgttgg
    17641 cgtatgatat tgattgttct catcagccag accaggaatg ttttgtaccc actcaggtac
    17701 accatcagta atatctacag caactgcgta atactcgtaa gctggtttat ttatggtaat
    17761 atcatgcatt aatgttggag cataactaaa tccttctttt atttcttgca tgatttttga
    17821 ttctgctttt tgacaatctg gacaatcagg tttgtaaaga acgatagtca gatttttatt
    17881 ttgcaatgga tcattcccat aattttcatc attttttact gtattaataa agttgttata
    17941 aacttcatcc ggcttttgac ctttattaat gtcttcgtat ttttgatact tttcagcatt
    18001 attttcagtc attgaagaca ttgttgttga tgccgatgta gtgctgtttt tagtaaccat
    18061 cgtactacta gactttgtgt ctttgttatt attagcacta ttagttgtac cacatgctgt
    18121 aagggttatt aatgctgctg tcgataacaa tacaaccttc aatttagttg atttcatttt
    18181 attcacctat ccttttttct tttgtttaac aataaaagaa taacccgtac tatccaataa
    18241 aacaacagca tcaatatgga aacccaaacc atttgtacaa tccaagcata aagactaaat
    18301 aataggtatt gtggattttt ttgaatgatt gtatttgaga tgccatttcc tacagtaaaa
    18361 tacattgtta cgaaaataac accttcaatt atcaagtgat ttgttggttg atttataatt
    18421 tttttcatat ttttactcct aaactaaatt tattcttcct gctaaatatt gaaaattata
    18481 tgggttctgc acatattccc taagttcctt aatacttgtt tcaatatcac caaaggctgt
    18541 tttaatgttt gctgacccat ctttatatac aatcatttcc ccttcaccca tacaaaaaga
    18601 aatttcagta ttagaattga ttgttttttc agcacgacca ttgataattt cttgaccatc
    18661 acgccatata aaatacataa gataaaatac ctcataagcg ttgtatccag tatccgttgt
    18721 cattgatacc aaaacatcag catcttgtat tcctgataaa gctaatccaa tttcatggat
    18781 cacatcaact tcattttctt cccaatctcc actttcgaag acggtatagg tttgattgtt
    18841 agcaaatggt tttctttcta tatcgaacat tcctttccaa tcaagaatca tgtacccgat
    18901 taaattgtga aagaaaatta gataaaataa atacaataag atgctaacta caaaaatatc
    18961 agccattatt gacataggat tatcaggata taaccagatg taaaacgatt gtttcaaatc
    19021 cattttttca tcatctcact ctacatattt atg
//
LOCUS       DBKOIJ010000044        18725 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000044 DBKOIJ010000000
VERSION     DBKOIJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.044"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            117..1802
                     /locus_tag="ACV5NJ_001197"
     CDS             117..1802
                     /locus_tag="ACV5NJ_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HHY6485299.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1816..3099
                     /locus_tag="ACV5NJ_001198"
     CDS             1816..3099
                     /locus_tag="ACV5NJ_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6485300.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3169..4038
                     /locus_tag="ACV5NJ_001199"
     CDS             3169..4038
                     /locus_tag="ACV5NJ_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485301.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4035..4871
                     /locus_tag="ACV5NJ_001200"
     CDS             4035..4871
                     /locus_tag="ACV5NJ_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485302.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4897..5964
                     /locus_tag="ACV5NJ_001201"
     CDS             4897..5964
                     /locus_tag="ACV5NJ_001201"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HHY6485303.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5961..6788
                     /locus_tag="ACV5NJ_001202"
     CDS             5961..6788
                     /locus_tag="ACV5NJ_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HHY6485304.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6833..7858
                     /locus_tag="ACV5NJ_001203"
     CDS             6833..7858
                     /locus_tag="ACV5NJ_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHY6485305.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7855..10038
                     /locus_tag="ACV5NJ_001204"
     CDS             7855..10038
                     /locus_tag="ACV5NJ_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HHY6485306.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10038..11168
                     /locus_tag="ACV5NJ_001205"
     CDS             10038..11168
                     /locus_tag="ACV5NJ_001205"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HHY6485307.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11165..11842
                     /gene="pgmB"
                     /locus_tag="ACV5NJ_001206"
     CDS             11165..11842
                     /gene="pgmB"
                     /locus_tag="ACV5NJ_001206"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HHY6485308.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11926..12453
                     /gene="msrA"
                     /locus_tag="ACV5NJ_001207"
     CDS             11926..12453
                     /gene="msrA"
                     /locus_tag="ACV5NJ_001207"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HHY6485309.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12654..13340)
                     /locus_tag="ACV5NJ_001208"
     CDS             complement(12654..13340)
                     /locus_tag="ACV5NJ_001208"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HHY6485310.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13420..14958
                     /locus_tag="ACV5NJ_001209"
     CDS             13420..14958
                     /locus_tag="ACV5NJ_001209"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - obsolete D-gluconate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HHY6485311.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15078..15545
                     /locus_tag="ACV5NJ_001210"
     CDS             15078..15545
                     /locus_tag="ACV5NJ_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HHY6485312.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16079..16507
                     /gene="rplM"
                     /locus_tag="ACV5NJ_001211"
     CDS             16079..16507
                     /gene="rplM"
                     /locus_tag="ACV5NJ_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HHY6485313.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16523..16915
                     /gene="rpsI"
                     /locus_tag="ACV5NJ_001212"
     CDS             16523..16915
                     /gene="rpsI"
                     /locus_tag="ACV5NJ_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HHY6485314.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(17008..18228)
                     /locus_tag="ACV5NJ_001213"
     CDS             complement(17008..18228)
                     /locus_tag="ACV5NJ_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6485315.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKTVFEAFQKGLKN"
BASE COUNT         6040 a         3412 c         3969 g         5304 t
ORIGIN      
        1 cttatttgta tttttgtgta tatcaaaatt atttaaaaaa atgctattga cagtaagcgt
       61 tttccataat atgatcaaat cgttgggaac gctcccaaaa aagaaagggg aatccgatgt
      121 atacaaaact tttacaagcg atttatcgcg actcagattt gtctgagaaa accgcctcta
      181 tgattgaaca aaaagtcagt gtttatcagc agaagaaagg ccaggcaggc aaactgcatt
      241 gggacgaaga agatattttc ttgattactt atggggatca attttttgaa gagcagcaac
      301 caactttgcg tacatttaag aaattttatc aaaagtattt ggcagataca tttagagttg
      361 tacatttctt gccatttttc ccatatacat ctgatgatgg tttctccgtt gtaaattatc
      421 aggcaatcaa cgaccgtttg ggcacatggg aagatataga agaaatgaga agcgaaacac
      481 ggttaatgtt cgactttgtc tgcaatcata tgtctgccga aagtacttgg tttcaggaat
      541 atttaaactt gaatgatacg tacaaagatt tttttattga tttgccactg gatacagata
      601 caagcagcgt gactagacca aggaccagcc cgttacttac aacgttcatt gacaaaaacg
      661 ggaaagaaaa acacgtatgg acgactttta gcgaagatca agtagacttg aattttaaaa
      721 atccagaagt tttattgaaa atgatcgata ttcttttgtt ttacttagaa aaaggcgctt
      781 catttattcg cttggacgct gtgggatttt tatggaaaga actaggaact tcttgtatac
      841 acctgccaga aacgcataaa atcatccaat tattccgtgc aatcgctgat gaagcagctc
      901 caggaacggt attgatcact gagacaaatg tgccacacaa agataatatc agttattttg
      961 ggaatggcga gaatgaagca cagatggtct atcagttttc gctaccacct ttgacccttc
     1021 aagcgattcg ttcgggtaat acaagtaagt tgaaagaatg gttaaaaacc atcgatttcg
     1081 catcgaaaaa aacaactttt tttaattttt tagcctccca tgacggtatc ggattgaatc
     1141 cggtacgagg tattctgccg gaaaaaacga tccttgaatt agtggaacag ctgaagaaag
     1201 agggtgcccg agtaaactac aagaaaaacc cagatggaac gaccagtcca tatgagataa
     1261 acgctacgta tctagatgcg ttaagttctc caacagatac gacggaatgg agaagcaaac
     1321 gctttttagc tgctcattca tttttggtaa cgttaccagg cgtgccggca atttatattc
     1381 aaagtgtgct gggaagtcga aatgattaca aaggcgtaga aaaaacagga gagaatcgaa
     1441 aaatcaatcg gcaaaaattt ttgttagcag atattgagaa agagttgcag acagatccat
     1501 taagaacagc agtatatggt gaattgaccc agatgctatc tattcgaaaa aaagaaaaag
     1561 ccttccatcc gaatagtttg atttacttac tggactcagc agattctttt atcagtttta
     1621 ttcgcgaagc gaaggaagag aaagtcttag ttatccataa tttttccaat caatcagcag
     1681 gttatctttt accaaaagga gattacctag acctgttttc tggacaaatt atccattgca
     1741 gtcaagaacg aaagttgata ttccagccgt atgaatatat gtggctgaaa ataataaaat
     1801 aagaggtgga aaaaaatgaa aaaaattatt gttactacct gtctgctagc tactggattt
     1861 cttcttactg cttgtggtga aagtcaaagc tctaacgaat cacaacagac aacgattcaa
     1921 ttcatgcact cttctgtcga acaggaacga ttaagtgtga tcaatgaact ggttgctgat
     1981 tttgaaaaag aaaatccaga catcaaaatc aagcaagtgc cggtagaaga atcagcgttc
     2041 aatacaaaaa tcgttacgtt agctcgttcc ggtaaattac cagaagtaat ggaagtcagt
     2101 caggattttg ctaaagtgat ggataaagat gaactgattg atcagacggc agtccaaaac
     2161 gttatcgagg aagctggaga agataattat tatgacggag ccaaaaacct tgttcgttca
     2221 gaagatggaa aaagctatat tgctgcacca attagcggct gggttcaagg tatttggtat
     2281 gacaaagaaa aattatcaga agctggtttt tcagaaccag aaaattggga tgatatttta
     2341 aaaattgctc aacatttcac agataaagag aataaacaat atggaattgc tatgcctact
     2401 gctgaaggaa caatgagtga gcaagcgttc tctcaatttg ctttgtcaaa tggagcaaat
     2461 gttttggatg atgaaggcga ggtaacgatc gatacggaaa agatgagaga agcattgtcg
     2521 ttttatcaaa acttatccca atacacgatg ccaggctcta atgatgtgac agaaatcaaa
     2581 gatgctttca tgaatggaac agcaccaatg gcaatctatt caacttacat tttgccttct
     2641 gtttatgaag cggggaactc agaaaatatc ggttttgcta tcccaacaca aaaagatcaa
     2701 gctgtctatg gcacagtatc tggattgacg atttcttctg gtctagacga gaaacaaaaa
     2761 gaggctgctg aagcattcac agctttctta tctcaaccga aaaacatgga aaagtgggtc
     2821 ttgatgtctc ctggtggtgc tcaaccagtg aataaacaag tagtcgaact ggatggttac
     2881 aaagaaaatg aagtcatcaa gtcttttggc gagctgccat ctgagattgc tagtgctttt
     2941 gatgaagtac aagtctttgg actagtcggg gaaaagaatt tcacgaaaat gggagatatc
     3001 acaagctcag gtgtgatcgg aaaagcagtg aaccaagtaa ctgtcggaaa cgaagatgtc
     3061 gatcaagctt tagctgacgc acaaaaaaat acgaaataac aagaagttct gccaatggtg
     3121 ttcgaaaaaa gtagatagct attggcagat tatcaggggg aggtttcaat gaatcgtcca
     3181 aggaaacatt ctgacttaaa attagccatg ggacttttag caccaagcat gcttttatta
     3241 gctgttttag tgctgattcc aatgctttcc aatattcaga tcagtttttt cgatatgccg
     3301 ctgaatccta actcagacgc cgtttttatc ggattaagca attatcttga aattttaagt
     3361 gatagtgaat tttatcgttc tttaggttta acagttgctt acacattctt agttgtagca
     3421 ggcagtacag ctttaggatt agccgtagct attttgctta accgcaagtt cagatttagg
     3481 aaaacggtgc gttcactgat tttattaagt tatgttacgc catctatttc tttgattttt
     3541 gcttggaaat atatgttcaa taatagttat ggaatcgtca atgatctgtt agtagatagg
     3601 ttgcagcttt tcaacgaagc accgctgtgg ttcgaccagc cagttgctag cttctttatg
     3661 gtcgttttgt ttgctatctg gagatactat ccgtatgcgt ttatttcatt tttggctatt
     3721 ttgcaaacag tcgacaaaac gctttatgaa gcagctgaaa tggacggtgc aaatgcttgg
     3781 cagaagttca agatcgtgac actgccggca atcaaaccag tactagcaac tgtgattgtt
     3841 ttacgatcta tctggatgtt ctatatgttt acagatgtct atctactgac gaataaagtc
     3901 aatatcttag gcgtttattt gtatcagaca gcctttgcgt tcaatgatct tggaaaagca
     3961 gcagctatat cgattctatt gttcatcatt attgcagcgg tcgtcttgct ctcacgaaag
     4021 aaggtggact tgaaatgaaa aataaaaaga gcaggaaaat cttattttac gcgacgattt
     4081 tgttgctatt agctgtcaat ttgtttccat ttttgatcat gctgatgaca tcattcaagg
     4141 attcacaaga agcaattgca acgacacaaa ctatttttcc gaaagaatgg acttttgaac
     4201 attatcagga tattttcaat gctgagattt ttccattcat catgtatttc aaaaatagtt
     4261 tagtgatctc cttgatagcc tccgtaactg cagtagtgat tgggatcagc ggggcatacg
     4321 ctttgtcaaa actacgcttc aaagctcgct cgaaaatcaa tgctagcttt tacaccgttt
     4381 atatgttttc cggtattttg atcgttgttc cattatttaa aatcatttcc ggtctaggtt
     4441 tgtatgatac caatacagca ctgattatca cgatggtcgt tcaaacactt cctactgcga
     4501 tattcatgct aaaaagttat tttgacacga ttccgactga tattgaagaa gcagcaatga
     4561 tcgatggtct tagccggatc cagatcatta ttcggatcat tataccattg gctatttctg
     4621 gaatcatttc tgtcttcgtt tattgcttta tggtttcgtg gaatgattat ttatttgctt
     4681 cgattttcct gtcgacctcc gaaaaattta ctttaccaat cggtttgaac acattattca
     4741 gtacaccaga ctacatttgg ggacgaatga tggccgcttc attggtgacc gctttaccag
     4801 tcatcattat gtatgcgtta tctgaacgat ttattaaagg tgatttagtc gaaggcggcg
     4861 taaaaggata aaaagaaaag aaagaggaaa gaaaagatga aaaaattagt tgcattagaa
     4921 ccaagaatag ccggattcaa agattacgaa gaccgtgaga tcgaaaagaa cgaagtacgt
     4981 atccgagtaa ttttcgctac tccaaaacca ggaacagaaa ttgtcgactt tagaggatta
     5041 agtccattta tcgatgaaga attttcagaa gaatggcgcc tattcttgcc aagagaagaa
     5101 ggaagccaac gcggtgtggt atttggcgaa tttcaattag gaaatatgat tgtcggtacg
     5161 atcgaagaaa cgggcagttt ggtgacagag tatcaaaaag gagagttagt ctgcacttat
     5221 ggacccattc gtgaaacgct gattacaaac ggtgttgata attataaact acgtaaactt
     5281 gatcggttgg atcaatggaa aaatgcagta tgttacgatc ctgctcaatt cgctttaggt
     5341 ggtatccggg aagcacatgt tcgagcaggt gatgctgtag caatcttcgg tttaggcgct
     5401 atcgggcaaa ttgcgattca attagctaaa aaagcaggtg ctactaccat cgttgggatc
     5461 gatcccatag cgcagcgtag agaattagca gaaaaacacg gtgccgatta cacgctagat
     5521 cctatgaaag cagatgtcgg ttttcgtcta aaggaattga cagataaacg tggagtagat
     5581 tcagttattg aaacgagtgg cagtgctgcc gcattacaag cttctttaat agggatcggc
     5641 tatggcggga tcatttctta tgttgccttt gctaaacctt ttccagctgg gtttaatctt
     5701 ggtcgcgaag cacatttcaa taatgcaaaa atcgtctttg ctcgagcatc tagtgaacca
     5761 aacccagatt acccaagatg gaaccgtaaa cgaatcgagg acacatgttg ggaattattg
     5821 atgaacggtt acttggattg tgaagaaatc attgatccag ttgtgccatt tgcagaaagc
     5881 gcagaagctt acatgcgtta tgtagaccag cacccagaag aaagtatcaa aatgggcgtt
     5941 caagtccgag gaggagaaca atgaaattcg gtacgcaaga tcaagcattt ttcccaagag
     6001 acattttaga aaaatttaga tttatcaaag actgtggatt tgaagaatac gaaatcgacg
     6061 gtaagttgtt aatagaaaat atcgacacag tcaaacaggc aatcaaagaa acagggatcc
     6121 cagtagtgac agcttgtaac ggctacgatg ggtggatcgg tgactttatc gaagaacggc
     6181 gtcttaatgg tgtacgtcaa attgctgaga ttttgcgtgc actaagcgag gttggaggca
     6241 aaggaatcgt tatcccagca gcttggggaa tgttcactta tcgattaccg ccgatggttt
     6301 caccccgaag caaagaagga gactataaag cagtcagcga atcacttcgt tatttagata
     6361 aagtagccga agaaacaggt acggttattt atttggagcc tttgaaccga taccaagacc
     6421 atatgatcaa tttgttagcg gatgcgcgtc attacattga ctctaatcaa ttgaaacatg
     6481 tgcagatcat cgctgatttt tatcatatga atattgaaga agatgatatt tcaaaagcat
     6541 tgactacgca aaaagagtat gtgggtcata tccatttagc agataatcat cgctaccaac
     6601 caggatctgg cgcaattgat tttactcgcc attttcaaac gctacttcaa aatgagtatg
     6661 aaggttatat ggtatatgaa tgtcgtttgc gaggagaaaa tgaagtgaaa atttatcagg
     6721 aatcattgca atacttgaaa aactgtctgg aaacagcgcg tcatttaaat gatttagaga
     6781 aagaatagag aaagaataac aataagtaaa taagcaaagg agagagaaat aaatgaagaa
     6841 agtagcaatc ataggcgctg gacaagtagc agaaaaagta catgctgctt attatcaaaa
     6901 aagatcagat gtcttagaat tggccgctgt ggttgatcca gtcagccagc gagcagaaga
     6961 cttttgccga cgtacaggat tttctcatgc gtatacatca gttacacaaa tgctgaatga
     7021 gatttctccc gatatcgtga gtgtctgtac acccaatcgc tttcacttcg aacatgtatt
     7081 ggctgccctc gaagctaatg cttcagtgat gtgcgagaaa ccaccagcta tgacagctga
     7141 tcaagcaaga aagatggcag agacagccga taaaaacggg cagattttgg cttatggatt
     7201 ccagcatcgt ttttctgacg aagcaacaat gatcaagaat catcttgatg ttctcgggga
     7261 tatttattac gtagaagcga atgcgcttcg tcggtctggt gttccaggct ggggaacgtt
     7321 tatcgacaaa gaacttcaag gaggcgggcc tttgatcgat tatggcattc acatgcttga
     7381 tacagcgatg tatattactg gatttcctaa agtaaaaagc gttcgggcgt acagcttcca
     7441 aaaaattggt acgaaaaaaa attcagggaa attcgggaaa tgggatccaa gcaaattcac
     7501 agtagaagat gcaatttttg gcacactgga attagaaaac ggcaagatca tccaactgaa
     7561 tacttcgttt gctttgaata tcagagaaga caaactgaat gtggcttttt gtggcgatca
     7621 ggcaggtgct aatctgtatc caggcgaact attcgaagat gtagatggaa agctggtttc
     7681 cttacaaaag accacgccag ttaatgaccc acatgaacta agtatggctc attttgtgaa
     7741 tcgggcgctc ggtgaccaga ctcagatgat tgcagatggc tggcaaggat atgtcatcca
     7801 gaatctggtg gaaaatttat atcgttcggc agaaacagga gaagttatta ccttatgaaa
     7861 gtagaagaaa aacagtttga tttagagtat atcaataagt atgcgagtca gatgaccatt
     7921 ggaaatggat tttttggtat cagaggaagc caggaggaag actatcggaa tcaagtaagg
     7981 ggaatgtttg cagctggtgt atataaccga cctttaggag cagaatcagc cgagttagtg
     8041 aatctcccag atgtcatcag atgggaaata aaaatcgacg gtgaacattt ttctcttgag
     8101 acgatgaaag ttcttgcata cgagcgggca ctagatttag aaatgggaga gcttaaacgc
     8161 tatgcgctca ttgaaactgc tcaaggaaaa aaagtggaag tcattacttg tcgtgttgct
     8221 gatcaaaata atctccatca ggcagccctg aaagtggtca tcaagccgtt gaccgaagac
     8281 tgccaagtag atgtgaaaac gggtatcgat ggacagcaaa ccaattttgg tacacagcag
     8341 ttggttgaaa aagaactgcg agtatttggt gacgatttga tcagtgcatc ctaccagaca
     8401 acagaaagta aaattgatat tgggattgca gctaaattca ataaagcagg tgtattccaa
     8461 gcaaaaaatc gccggatcac cgcttccttt tcagaaaaga tcagaaaaaa tacgtccttt
     8521 acgctggaga agatcatcgt gctctctacc tctcttcagc aaacagaccc gtttaagaaa
     8581 agtattgccc aagcccagaa aagcgttagt tatacagaaa ttttagagca gtcgatgtct
     8641 tattggacag atttttggca tcagcatcgg ataacgatcc aaggcgattc ttctttcgat
     8701 cagttagcga tggacttcgc tgcttaccat ttacagatta tgactccccg caatgatcca
     8761 agtgttagtg taggggcgaa gggactaact ggcgaaggtt ataaagggca tgttttctgg
     8821 gatacggaaa tttttatcct gcctttcttt ttatatcagt ttcctgaagt agcaaagaat
     8881 ttgctgctgt accgttatga tcgattagaa ggcgcacggc aaaaagcaag agattatcac
     8941 tatcaaggtg cacttttccc atgggaaagc gcacacactg gtcaggaaga gacacccaaa
     9001 tttgctgcat tgaatatcaa aaccggaaca agacaagaag ttgcttcagc tgccgcagaa
     9061 catcatatca gtgcagacct tgcttatgcc gttcaagagt atgtccaagc aacagaggat
     9121 caatcgtttt tagaaacaaa gggaaaaacg ctcattcatg aaacagcagc tttttggctg
     9181 agcagagctg taaaagtcga tgatcattta gagatacaca gagtcattgg tccagatgaa
     9241 tacacagaat atatcgacaa taatgcatat acgaactatc tggcgcatta taatgtggcc
     9301 ttagctgtta aatatcaagc tggcgatccg ctttttttga aagaatgtca aaatttttta
     9361 gataagttat atttgccagt gcctaacaaa gaacagctga ttccgcaaga tgacacgtat
     9421 ctcagtaagc cagttattga tttgaccact tacaaagaac gtcctggcag ccagtctatt
     9481 ttacttgatt atgcccgtag cgaagtaaat gaactccaag tcagcaagca agcagatctt
     9541 gtcatgctgt tatacttatt acctgagtat ttttctcaac caatcgtttc taagaatttg
     9601 cattactatg aaaacaaaac aatccacgac tcatcgttga gtaaagcgat tcacgccata
     9661 gaggcttgcc gtatccaaga aatggaatgg gcgtatcatt tattccaaga agcgtgcctg
     9721 attgatttag gaccagatcc gcattcatca gatgacggaa ttcatgctgc ttcattagga
     9781 gcactatggc tagcagttat atttggtttt tcaggtatta cagtaacgaa tggaactctt
     9841 tctatttctc caagacttcc ttcagcatgg gaaaaaattt cttttccttt tatttggaaa
     9901 aatcacaggt tgctatttgt tttgacaaaa aaacaaatag taataaaaaa agagacgaaa
     9961 gaaacattac ctatttttgt caatggagac caataccagc tggaaaaaga actgatcttg
    10021 gaacggaggg gaaaataatg cgtattctga ttgcgccaga ttctttcaaa gaaagtctga
    10081 cagccttaga ggcagcacaa gccattcaat caggttttca agaagttttt gttcaggctg
    10141 aatatgatct gctacctata ggcgatggag gagaaggaac ggtttcatca ttagcagaca
    10201 gtttaaggtt gaagcggcaa acagcagtcg ttactaatgc atttggcaaa aacaaagaaa
    10261 tccagtacgc ttcagacggt caaactgcga tttttgaaat ggcagaaatc gtcggattga
    10321 aagatgtccc actaaaccaa caacagccac ttaggttatc cacgcgagga gtaggagaac
    10381 tgatacgaca attgttgcaa caaggtgttc gacacatcat catcggcgtt ggcggaagta
    10441 gtaccaacga tggcggcatc ggcatggcag aaggtctagg ttatgctttt tacgatcaag
    10501 ataatcaaaa agtagaagca ctgggagaaa atctaggtaa aattgctaca gttgataaaa
    10561 cgaacgtgcc tattgagctg attaaagcca aaatcactgt cattactgat gttactaatc
    10621 ttttatgcgg aaaagaaggc gcttcttttg ttttcggacc acaaaaaggc ttgaaagaaa
    10681 atgaattagc agtggctgac caagccatgc atgattttta tatgagattt gcccctcaag
    10741 taatcgaggc atcgggcagt agtgctggtg gtggaatggg tgcaggactg ttagcatttg
    10801 cttcagggaa aatagtctct ggcatcgact acgtattgga tttactggag ttcgataaac
    10861 gggtacagca agcagacctc gtaatcgtag gcgaaggacg tctagatgga caaagtctaa
    10921 aaggaaaagc gccagttggg attgctagaa gagtaccaga gggcgttcct gtgatagcta
    10981 tttgcggaag tgttggtaaa ggaagcgaaa aagcagcaga tgttggcatt tcggccgttt
    11041 ttccgatcct tcctgcagct gtgcctttac aagaagcttt acagcaagcc actgataatc
    11101 ttcatcgcac agctaaaaac atcgcatctt tacttcaagt cgttcatttt caaggaggta
    11161 atcgatgaaa atcaaagcag cactattcga tttagatggc gttcttgtag acacagctcg
    11221 ttatcattac gaagcgtggc tggttttagc taaccagctg tccattccat tcactgagaa
    11281 agaaaacgag caactgaaag gaatatccag aaccgagtca ctagagcgat tgctgtcatt
    11341 tggaaaaatg gaacagaaat tctcagaaaa agagaaatca gcctttgctg agcaaaaaaa
    11401 caatctatat cttcaagcga ttcaaaaaat ggatgaaaca tccgttttgc ctggtgcaat
    11461 agctgttctt gagtacttga aaaaaacaaa catcaagatc ggattgggat cagcaagcaa
    11521 aaatgcacgc ctcattttag aaaaaacgaa tttgacaagt tattttgatg tccttattga
    11581 cggcacgcaa gtatcaaaag caaaacctga tccagaagtt tttctcaaag gagcacagca
    11641 attgaatgta ccgccaaatg catgcttggt cattgaagat tcagaagctg gttgtcaagc
    11701 agctttggct ggtaacatgc atgtattggg aataggtgaa aacataaatc ttccttctgc
    11761 tgaatatgtg attcctgatt taaccgtttt tgatcaagta cgttcgtttt ggcatttatc
    11821 agaagccgta tcacgtatat aatagtttcc gttttttctg tcacaagcaa aacgttatcc
    11881 tccttttgga aatcaaggta tgatgagaaa aaagggggat tttatatgac aaaaaaagca
    11941 gttttcgcag gcggatgctt ttggtgtatg gtcaaaccat ttgaagagca gccagggatc
    12001 atttctgtga catccggtta tactggcggg catgtaccaa atccaactta tgaacaagta
    12061 acaacaggca caacagggca tacagaagca gtagagatcg aatatgatcc agaagttatt
    12121 tcttatgatc agttggtgga aatctattgg cagcaaaccg atccgactga cgcattagga
    12181 cagttcgctg atcgcggtga ttcttatcgt ccagtgatct actataatga tgaagaagaa
    12241 cgaaatattg cagagcgttc aaaacaagca ttgcaagaca gcgggcgatt cgataagcca
    12301 atcgtgacaa aaatcgaacc aagaagcgaa ttttatcctg cagaagatta ccatcaggat
    12361 tattacaaga aaaacaagct ccattacaat ctttatcgag aaggttctgg acgtgcaggt
    12421 ttccttcgtc gacattgggg cgtcaagaaa taagaagccg aaaaagctac ttatggaaca
    12481 ccgtatatca gaggttgtaa aaccagcatt ttgagctaat actgagggtc agctgattga
    12541 acaccattta gtggagaggt tgtgaacctg tcgcctagct ccaagtcaca aggatttggt
    12601 aagagaacac tatatatcag aggttgtgac aagtcgtttg tcacaacctt ttttcaatac
    12661 ctaagatgaa ggacaatcag caacgcactg ctcaaaaaag gaatgaaagg cagcttttcc
    12721 aacttttttt tatgacagat aaagtaaaca gaaaaaacgg atattccaaa cagactagca
    12781 agaaacaaga tctgaagcaa ctcttctaga gacaagccac cactccaaga aagcatcaac
    12841 agagtatcgc cagctccgag ataggattga ccatagcgaa ggcacacacc gcctacgata
    12901 aacgttaaca gcacactttg ccattggaag acgccggcct gcaaccaaaa aatccataaa
    12961 attgcccaag aacagaatag cgtgtgagaa tggaccatga agtaaaataa atccatcaac
    13021 gaaaatagca aagccgaaag catccatatg aacgtcgaaa aatttcttgc ggaagaaaaa
    13081 gtaaagaacc aggcaaaaag accaccacat aaggcttctg ccaacaaata gtagaagggc
    13141 aaccgacacc tgcaatagcg gcagcgaaat cgttggacga tgatcgagag aatcggaatc
    13201 aattcatacc aagaaagcga acgacagcat ttgacacaat gagatctagg ataaataata
    13261 gagtgaccta acggaatccg ctgggcggtt aaacataaaa atgatccgat acagcaacca
    13321 atgataaaat aaagtaacac gccatctccc ccttttcaaa accaagatac gcaagcttaa
    13381 actaaatttt tgtacataca taaaatttga ggtgaaaaaa tgaacgtttc aatcggatta
    13441 gatatcggaa ccacacaaac caaagcagtt gctttcacag atgaagccca agaagcagca
    13501 tccgcttatt ttcgctaccc tttgatccaa gaaacacaag ggatggcaga acaagatcag
    13561 gaattgatct ttcaaggtgt ctgcaccgtt ataaaagaag taattaaaca gttgccttca
    13621 gacacaatca ttcaatttgt ctccttctcc tctgcgatgc atggcctgat attgttggat
    13681 gaagagaaaa caccgttatc tcgaatgatc acttgggcag ataaccgagc agcggacgaa
    13741 gcagaaaaac tgaaatatac cgtgcttggc aaagaatttt atcggcagac tgggacgccg
    13801 attcatccaa tgacaccatt gaacaaaatc aagtggctgc aaaacagcca acctgaatta
    13861 ttcaaaaaaa ctgcttattt tgtcggtatc aaagactata tcttttatag attgtttggc
    13921 gaactgatca gcgactacgg aacagcttca ggaactggat attttgatat ccatgaattc
    13981 caatgggcag aagaagtgct tgactacttg aacattcgaa tagagcaatt gccgcaagtc
    14041 tttccttcca cctatcaagt aacaggtcta ccatcaaaaa cagcagcaga actaggtttg
    14101 ccaaatgata tatcatttgt tttgggaggg gcagacgggc cgctttctaa tctagggtta
    14161 ggtgcttttg gtgatacgat cgccgctttg accgtaggaa cgagcggagc acttcgtttt
    14221 attactactc agcctcagtt ccatccagaa atggagacgt tttgttatgt tctggatcag
    14281 aaacattggg tagtgggagg agcgactagc aatggtgctg gcatctttga ttgggcaggg
    14341 catacatttt tacaggaagt cgttcaatct gctaaagaaa aaggagaaaa tccatatgat
    14401 gcactgcttt cggctatcgc ttttgtgcca gcgggtgcca atggtctgat ttttcatccc
    14461 tatttacttg gtgagcgagc accgctttgg gacgcagatg cgtttggcag ttttatcggt
    14521 ttacagcggc aacatacaga aaaagaaatg atgaaggcga ttctagaagg gatctgtctc
    14581 aacctttatc ggattttaaa aggagtttgc caggaaaatc agctcacaga gatccgtgct
    14641 acaggcgggt ttgctcaatc tccgattttt cgtcagatga tggctgatgt actgggttac
    14701 ccgcttgttt tccctactgt cacagaagca tccgcattag gagctgtgat tttaggctgg
    14761 agaagcatcg gaaagatcca aagtatcagt gaagcacagg aattgataga attgaaagag
    14821 caagttggag tacaagaaac tgatcataaa aaatatcaga aaatctatcc attatttgtt
    14881 tcgacacaaa agcatttagc acagacttat cagcaatttg ctcaactgcg agaagaatta
    14941 gctgattctg aaaaatgatt ggctttacgg ataaaaaagc gaactgaaaa tcgttctcag
    15001 gtaaatgatt ttgttgtact tttcattgaa agtctactat tattttaagt gtaagaaaat
    15061 gttaggaggc aagaaagatg aaattccaac aaaccaaaga agtattaaat caattagttg
    15121 cagatctaag ccaaatgtct gttgttgttc accaaacaca ttggtacatg agaggaccag
    15181 gtttccttac attgcaccca atgatggatg agttcatgga tgatttgaac gctcagttag
    15241 atgaagtatc cgaacgcttg atcacattag acggttcacc ttattctaca ttgcgtgaat
    15301 ttgcagataa cacaaaaatc cctgatgaaa ttggccgctg ggatcgtact attccagaac
    15361 gtttggaaac acttgttgca ggctatcgtt atctagcaga tctttaccaa aaaggaatcg
    15421 aagtttctgg agaagaaggc gacgatccaa cacaagatat ctttatcgaa ttcaaaacag
    15481 ccacagaaaa acgtatctgg atgatccaag ctcatttggg caaagcgcca gagatcgacg
    15541 cgtaaaaact gttgttgaac ttgaccgaaa aagaggttgt gaaatcagcg ttttgacctg
    15601 agtattagag aaacaaacgt caaaatagtt cctcatattt tgccgttttt tgagctaata
    15661 ccgagggtca gctgattgaa caccgtttac caagaggttg taagcctgcc gttcagctcc
    15721 gagtcacaag aaacaatttt aaaaaacagc ttcttatgtt ttatggatat tgtgacttgt
    15781 gaccgaggag ttggcaggag aacaccgttt attcaaaata aggggttgtg acaagacttt
    15841 tgtcacagcc tctttttgtt tttcattttg acaggaaacg acccttttat ataaaagaaa
    15901 gaaaagaagt aaagtttcat ataaattttt caatcaagca gaaaaacgtt gacattgact
    15961 agattagcgg gtaatatgtt aaatggtatt gtttgcccca caaagagccc cggaaactcg
    16021 aagtgtatgt caaacatcag aacttaaatt ggaggaaaat aacgtgcgta caacatatat
    16081 ggccaaacca ggcgaagtag aacgtaaatg gtatgtagta gacgcaactg atgttccatt
    16141 gggacgtctt tcaacagttg ttgcatctgt actacgtggt aaaaataaac caactttcac
    16201 acctcatttg gatactggcg atttcgtcat cgtcatcaat gcagacaaag tgaaattaac
    16261 aggtaaaaaa gcgactgaca aaatttatta ccgtcacagc aactacccag gaggattgaa
    16321 atcaatcact gctggcgaat tgcgtgctaa aaattctcgt cgtttgatcg aaacttctgt
    16381 aaaaggtatg cttcctaaaa acactttagg ccgcaaacaa tttacaaaat tgaacgtata
    16441 cggtggagca gaacatccac atgcagcaca acaaccagaa gttttagaca tcacaaactt
    16501 aatctaatag gagggaattt cattggcaca agttcaatat atcggcacag gccgtcgtaa
    16561 aaacgcagtt gctcgcgtac gtctagtacc aggaactggc aaaattactg taaataaaaa
    16621 agatgttgaa gaatacattc cacatgctga cttgcgtgaa gtaatcaatc aacctttcgc
    16681 tgttactgaa acaaaaggcg catatgacgt atttgttaac gtaaacggcg gaggatacgc
    16741 tggacaatca ggagctatcc gtcatggtat cgctcgtgca ttgctagaag ttgatcctga
    16801 cttccgttta gctcttaaac gtgctggctt acttactcgt gacgcacgta tggtcgaacg
    16861 taaaaaacca ggtcttaaaa aagctcgtaa agcttcacag ttttcaaaac gttaatattc
    16921 tttgtatatc aatgtttcaa gacacttttc aattatttgg aaagtgtctt tttttagttt
    16981 aagaacacat tcgagaacac attcatccta gttctttaat cctttttgaa atgcttcgaa
    17041 aacagtctta tcaactacat ctggtgtgtt tacgtatatc tttgtagtag aaatatctga
    17101 gtgtgttaaa gcattagata tttgattcat atttgctccg ccttgtctag ctatagttgc
    17161 ataggtatga cgaagcttat gtggagataa atgagccaat tcaggatgtc gtcttttaat
    17221 tgaattgatt cgatagttta ggtaatcagc gtgaacaggg caatttactt ctccggatgg
    17281 tttagtatag gtaaagagat actgatctgg tgtttgatta atttttaatc tcaacaattg
    17341 ctcagcttgc actgatttcc atttgcttag taatgccatt acaatttcgg gtacttgaat
    17401 tacggtatcc tttcgacctt ttgtaaattt ctttttttga tatttatcca aagcatgttt
    17461 aaggcgaacc gtttgtttct caaagtcgat atatttccat tgcaaagcat acgtctcact
    17521 ttttctatca ccaagatata gggtaagcat gaaaagagta tagtcatgaa gagtaagcaa
    17581 gtgattattc aagtcagtgt ttactgcttc tatccaagca ataagctctt ctgacgataa
    17641 tgcttgatta ccttcccgta tccttctttc ttctaaagca atttttttag gtgcagtgat
    17701 acattgaata attttggtag ttcggttaaa ttcaatataa tttagtattt ctgcaatatc
    17761 aaacatagaa cggacatatc ctttgataat ttttatattt gcgtattctt tcgatttttt
    17821 tgttaattca tctgatatta tctcagtatt tgaatttaga tagttcattg catactcacc
    17881 aaacattgga aggatgtgca atcggaatat atctttagta ttactaattg ttacgtaact
    17941 tggagctctt tttgatctag tagtctgacc tagttcatat ctaggaagcc attttgattc
    18001 atagaattcc ttaaatttta tttttccttt taattctagt gaatttgcat tctcctctct
    18061 taaaactgtt tcaattttcc ttttcattgc attttcggct ttaatagcca attgcctagt
    18121 tggaaatact ttttgatact tttctgatgt aattccagtg atttctctga atttttttgg
    18181 atacctcaga ttagttttat atttcccatt tgaaagtttt gtaggcaaaa ttcctcactc
    18241 ccttaaaaag taaggcagta tatttcgcta ttcttagtat atgcggcgag aagaaatttt
    18301 caaattgttt taaatgtatt cataaaatct ttgatatcct ctatgtcaaa gcgaactata
    18361 ccgtcaattc tgattactgg aaaattatat atacgaatcc acttatctaa agtattatta
    18421 tcaattccta gaaactcaca ggtttgtttt ttattcaagt agactctcaa ttttataaaa
    18481 tcatctccta aaaaagatta gaatttttga attgctttgg gtaagcacat aggaatttca
    18541 cctctcccca gtttacggcg agccttacgg aagtatcatt atttgctgct attgtcatgg
    18601 cattttgttt tcccagaaca aaaagaaatc tctaagtatt tgtcgctaat caggtcatgg
    18661 cgtacttgag tttctagctc gaatagcagg gatcgttatt caaaaattgt atttaattgt
    18721 caatg
//
LOCUS       DBKOIJ010000045        18542 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000045 DBKOIJ010000000
VERSION     DBKOIJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.045"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(273..1592)
                     /locus_tag="ACV5NJ_001214"
     CDS             complement(273..1592)
                     /locus_tag="ACV5NJ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HHY6485316.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(1749..2135)
                     /locus_tag="ACV5NJ_001215"
                     /pseudo
     CDS             complement(1749..2135)
                     /locus_tag="ACV5NJ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2320..2718)
                     /locus_tag="ACV5NJ_001216"
     CDS             complement(2320..2718)
                     /locus_tag="ACV5NJ_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HHY6485317.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(2715..3488)
                     /locus_tag="ACV5NJ_001217"
     CDS             complement(2715..3488)
                     /locus_tag="ACV5NJ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HHY6485318.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(3485..4168)
                     /locus_tag="ACV5NJ_001218"
     CDS             complement(3485..4168)
                     /locus_tag="ACV5NJ_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HHY6485319.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(4335..5222)
                     /locus_tag="ACV5NJ_001219"
     CDS             complement(4335..5222)
                     /locus_tag="ACV5NJ_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485320.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(5219..6088)
                     /locus_tag="ACV5NJ_001220"
     CDS             complement(5219..6088)
                     /locus_tag="ACV5NJ_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485321.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(6225..7511)
                     /locus_tag="ACV5NJ_001221"
     CDS             complement(6225..7511)
                     /locus_tag="ACV5NJ_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6485322.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            7784..8362
                     /locus_tag="ACV5NJ_001222"
     CDS             7784..8362
                     /locus_tag="ACV5NJ_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HHY6485323.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            8362..9291
                     /locus_tag="ACV5NJ_001223"
     CDS             8362..9291
                     /locus_tag="ACV5NJ_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6485324.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            9288..11024
                     /locus_tag="ACV5NJ_001224"
     CDS             9288..11024
                     /locus_tag="ACV5NJ_001224"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing sensor histidine kinase"
                     /protein_id="HHY6485325.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(11087..11923)
                     /gene="pheA"
                     /locus_tag="ACV5NJ_001225"
     CDS             complement(11087..11923)
                     /gene="pheA"
                     /locus_tag="ACV5NJ_001225"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HHY6485326.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(11920..12429)
                     /locus_tag="ACV5NJ_001226"
     CDS             complement(11920..12429)
                     /locus_tag="ACV5NJ_001226"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HHY6485327.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(12422..13717)
                     /gene="aroA"
                     /locus_tag="ACV5NJ_001227"
     CDS             complement(12422..13717)
                     /gene="aroA"
                     /locus_tag="ACV5NJ_001227"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HHY6485328.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(13730..14821)
                     /locus_tag="ACV5NJ_001228"
     CDS             complement(13730..14821)
                     /locus_tag="ACV5NJ_001228"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HHY6485329.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(14838..16004)
                     /gene="aroC"
                     /locus_tag="ACV5NJ_001229"
     CDS             complement(14838..16004)
                     /gene="aroC"
                     /locus_tag="ACV5NJ_001229"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HHY6485330.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(16006..17070)
                     /gene="aroB"
                     /locus_tag="ACV5NJ_001230"
     CDS             complement(16006..17070)
                     /gene="aroB"
                     /locus_tag="ACV5NJ_001230"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HHY6485331.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(17103..18125)
                     /gene="aroF"
                     /locus_tag="ACV5NJ_001231"
     CDS             complement(17103..18125)
                     /gene="aroF"
                     /locus_tag="ACV5NJ_001231"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HHY6485332.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         5385 a         4020 c         3312 g         5825 t
ORIGIN      
        1 aggattgatt acaaatattg ttttattagt ctgtacacca aatgaaatat ttgtaaaaaa
       61 gataggcagc ctgaataatt catagcttta attctttggc acattttatt gaaatatgta
      121 tcactaatat ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca
      181 aaaatattga ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa
      241 cttcccagta aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg
      301 cgagcaaaga ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag
      361 actcgtctac ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa
      421 tgaatggtca ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag
      481 ttgtaagcta tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct
      541 gttttgtcaa agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt
      601 ttggcataga tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata
      661 aattcatgtc gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga
      721 ctttgtgacc aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc
      781 cacttttgat tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta
      841 ttcttcagtc gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt
      901 gtagcgaacc cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag
      961 tgctctaata aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa
     1021 cgtagttcag cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa
     1081 ccataggttt ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa
     1141 tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg
     1201 ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa
     1261 aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca
     1321 ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata
     1381 atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg
     1441 tgaatgtgtt gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg
     1501 acgagacccg aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg
     1561 aattttactt ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt
     1621 tagtcacttt aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga
     1681 aaaagtaatt gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt
     1741 attcaggtta gaatcttttg ctgacgtcta aaaatagaaa aagaattttc gtcatctatt
     1801 tgataacgca tttgtttttg cagttctgct gcagtcagat ggatcttccc atcgtttgta
     1861 tcgatcatta aaccttctgg aaacagttca accactgctc gatcccaaga ttcaagagaa
     1921 agctcgatat aatcttttac tggtaattcc aattctacct taaaaaagtg caaaacagcc
     1981 gacagccctt ctgcttgata gatttctcct aatgttagcc cctctagatg gaactctggt
     2041 gaaaaatgca gcaatgtttt attttcctgc aattgaacat atagaaatgt agatcgctca
     2101 atcagagtat cttttaaaac aactaaaaaa ttcatttttc tccccctttt ttcatcattt
     2161 ttaaaatagc atattttgaa aagaaagtct gatgcttaga gtagaatttc ataaaaattt
     2221 actatttcat aagtattcac gagaaaaata caaacaaaaa agcggaaata acgctcatcc
     2281 gccttcttgt ttgtgtttat ttcctcttca aacgcttgtc tatgattcag acttttctct
     2341 atcgttattt tcatctaaaa gttcttgcat tccgataatt tctcgatatt gatgaatctt
     2401 agtgatacca atgactttgt ctacatcaat actaaaacaa ataccattac ctggaatatc
     2461 gatgttgatg ccttgaccaa tcgcatccat tactttattc gtcaaactat cgggtactaa
     2521 tgtcaacaca atatcttttt ctggcgaaat gtccaaacct aaaaatttcc ctgaatcatg
     2581 tacaccagaa cctcttccat gtaagatcgt tccgcctgct gcacctgctt cttttgaaaa
     2641 gtcgataaca tcttcgccca ttcctccgtc aacgatcgtc acaatcatgt gaagattcaa
     2701 tgctacaaga tctttcattt gctttccccc aatttttgca atctttcttt ttcgattctg
     2761 tctttttcat cttttcgttt ataaatatat cctagtacta aagtggataa tattggtgtc
     2821 aatgcaatca ttgcaaccac accaaacccg tcaatgatcg gatctcttcc ttcaatttgt
     2881 gcagcgattg ccacgctcat tgataaaata aatgtcgaac acatcgggcc cgttgctact
     2941 ccaccgttat caaaagccat tgctaaaaag atcttgtcca cttttcgtcc taaaataaat
     3001 gctagcccgt atcctgggat caagaaataa taaagagaga atccgacgag caaacgccac
     3061 atagacaatc ctactgctgc gccgatccca attgaaatta ccatcagcat gattttcgat
     3121 cggattctcc cctcgctcaa ttcttcaacc tgtttgatca ttacatgaat cgccggctca
     3181 gcaaacccta ctaagaaccc cattaaaaag ccaaggggaa ccagtatata attgtaaggc
     3241 aatcccgcaa tagcactgcc taaatgctgt cctaccggta cataagccat attgactccg
     3301 tgcaggaaaa ggatcaaccc aatagtcgtt atcaagaaac ctttcataat gtcaaagaaa
     3361 cgctttttgc gcatacgaaa actaatgata ttcattaaaa caaataggat tacgataggt
     3421 gccactgcca aaatgacttc catggtaacg atttcgattc catctatcac ttgctgccag
     3481 aaactcattg gaagatcacc cccaaaatca tcacggcaag aatcggccct aacgaagcaa
     3541 taccgaccat tccaaaactg tcattttcat ttttcttttg tttgatcata ctagtcatcc
     3601 ctccggcaag ggccaagata aatggaacag ttactggacc agtagtcact ccccccgaat
     3661 caaaagcaat cggcataaat tcggaactgg tgaaaaatga ggcgatcaaa acgcctatgt
     3721 agcctatagc catcaattga taataggata gcttgaatac gacccgaagc agcgcaaagg
     3781 ctagaaatac tccagtgccg acgctgacga tcccaatcag caacactctg ccaatctctc
     3841 cgtcagatat atcatatact tgttgtccca aaacttgcac ggaaggttca gcaatcgtga
     3901 tcacgatccc gatagcaaat cccaaactga ccaaaacggt caaacttttc tttttgatca
     3961 tatatttccc tactagatct ccgacttcca tcatagaata atctgctcca aacaaaaaga
     4021 gcgtcattcc gatcatcatg attgccgcac caaccagaaa tgaagcgagg tcttctcctt
     4081 ctaacggtgc aaaaacaaac gttaaaatga caatcaggac agtcatcggc aatatagcag
     4141 caatcacttc tttaaaattt tttagcatgt atcctcctcc tttactcttt aagtttacct
     4201 taaaatgaaa gagttttcga gcacaaactt aaaacgttat catttaattt ttctcaacat
     4261 aaaataacac acgtcccaaa agtatctttt tacttggaaa aatgactttg aaggaacgcg
     4321 cttattgtgt ttttttattt tacagagccg atcattcctt gaacgaattg cttttgcaaa
     4381 acaaaaaata cgatggcagt tggtaaagta gcgatgacta atccagccat gatcattcca
     4441 tagtctggtg tataggaagc acccatagca gataaaacaa gcggaaccgt ccgtttatca
     4501 ggagattgca aagctactaa gggccataaa taattgttcc agctactcat gaatgtgatg
     4561 attgcagccg cggcataggt atttttagcc gtcggcatat acactctaaa aaaaatactt
     4621 agttctttta gaccgtctaa tcgagcggct tctactagat cttttggaaa agatttaaca
     4681 ttttgtcgaa agaagaagat caaaaaagca gtgctaacag atggcaggat gacaaccaag
     4741 aaactgttga ttcccaaaac cgttccgttc aattgtgaaa acattcgata taaagggatc
     4801 atcaatgccg caaaaggaac catcatcgat agcagtagaa tattgaaaat taggtctttt
     4861 cgtttacttt gataaatctc aaatccatat cctgccattg acgaaatcag caatgcaaac
     4921 acagtggtca ccaaagcgat catcgctgaa ttccaaaggg aacgagtaaa attcagctca
     4981 ttggaaaaca aattttgaaa attgatgatc aagttactgc caatctttat ttttcccgat
     5041 gtgatatcca cgggtgtatt cgtcattccc aaaaccatcc agacaaaagg gaaaatggaa
     5101 ataaatgata ggatgcttaa tgctgtatac tttaatactg acccaattgt cgaacgaagt
     5161 tctaatgctt gttttttgac gttggggcgt tcgattgttt ctttttctac catatttttc
     5221 atcgactttc tcctcctact ttcgtttgga ttatcgacaa tatcacaata aaggaaacaa
     5281 taacaaagga aactgctgcc gcatatccaa aattaggcgt gaatttataa ctcaaattat
     5341 agatgtattg agatagggtg gtcgttgcat ttgctggtcc cccgccagta atattttgta
     5401 cttcatcaaa aagctgcaat gtcccgatcg tcgaagtaat ggaagtaaac aaaatgattg
     5461 gttttaaatt tgggatcgtc actttgataa actgctgcca tttagaagca ccgtcaatag
     5521 atgctgcttc ataaatctcc gaatcaatat tttgcatgcc tgaaaggaaa aaaatcatat
     5581 tgtatccagt ccaacgccaa gtgatcgaaa gaatgatcag aactctcgcc caaattggat
     5641 gagtcaacca gccaatcggt gtgtcaatca taccaatgcc caacaacaag ttgtttacaa
     5701 ggccagattg agaaaataga cttttcatga tcagagaata actgaccatc gaagtgatac
     5761 atggaagaaa gatagcggtt cgaaacaacc ctttatattt caacttttta tcatttaaaa
     5821 ggttggaaat aaaaagtgct agaaacaaca tgatcggtac ttgtacaagt aaatacagga
     5881 atgtattaaa aaaagctttt ttaaaagttg catctgtcaa catccgctga taattaccta
     5941 acccgttaaa tgtcatattg acagggggac cactttgaaa agacgtgatc aaagccaaaa
     6001 ccatcggaat aaaaaccatg attgatatca aaatgactgg caagattaaa aaagcatgcc
     6061 cgtttttgaa taaatctttc tgcttcattc ttttccctcc aattctatca ttgattatct
     6121 acatgaaaag acaagaaaga caatatctgt aaggcagacg tgatcttttt tgtcttttcg
     6181 tttgtctgat gaaaaagcga agaaattcac ttttttacca ccgatcaatt agccaattgt
     6241 gcttctactt gtttttgtgt atctgctagt accttgtctg tctcttctcc attgatgatt
     6301 cgatgaagag cttcagctac gactgcttca attgcatagg tctcatgtcc gtaattcact
     6361 tctggtattt cagatgtcca tgttgaaaaa tcgtcaaaga tcttttgacc actgtaaaat
     6421 tcagaagctt cttgataagc ttcttgttca ttcgcgctaa gcatcgtaga tactagtccg
     6481 atttctttcg caagagtgcc catcagttct tcattcgaag caaacgttgc ttctaggaaa
     6541 tctttcgctg aatcttctcc agctactcct ttgatcacat accagccagc gccgccaagg
     6601 ttagaagctt gcgctttttg cagattttct ggaagtgcag gtattggtgc aatcttccat
     6661 tttccagatt ggttttctgc tccttggatc gttgaagaat accaagcgcc agttgggcta
     6721 gaagctactg ctcctgattg tatcgcagtt actccagcat tccagtcaga ggtttgttcg
     6781 actagatctt ctttcaaaag agtggcaaac aattcaagac catactttaa agatgcattg
     6841 tcttggatcg tgactgtctc cccatcttca gatgtatacc attctcctgc ctgttgcata
     6901 atcatcctta ctcgaccaag gtcagaagga ttgacttcag cgattttctt tcccgtcttt
     6961 tctttgacat ctcgcgctac ttgaatatac tcgtcccact tcagattctc catatcctct
     7021 tctgtataac ctgcttctgc catcagatcc gtacgataga agtttgctgt tacgcccgaa
     7081 tcgaaaggaa caccgtatat tttatctcct actttgttta ccgcaaattt atacggtgca
     7141 aaatgatctt cctttacaat agcagacaaa ttagcgaatg catctggata agaagtcaaa
     7201 tagccttgta tcctataatc ttcaatcaag acgatattgg gcaatccttc tgtattcccg
     7261 ctcgctaaag cggtgttcag tttctgaacg atatcatcct gagacatcgt aacaacttct
     7321 atctctgttt cttcgttctc ataaactttt tttgcttcat tcactgcttt gatattgaat
     7381 gtctcatccc acgcccagac agtgactttt tcttctttgt ctgctgtact actagcactt
     7441 ccgcatcctg ttaagaaacc tgatgctgcc gctaaacaaa cgactccagc aattaacttt
     7501 ctgatgttca tttttatccg ctccttttcc atccattagt tgtttacgtt tgctacattc
     7561 atcataccaa aatttgaaaa cggtttcttt attattcaga tacttcaaaa atttctccta
     7621 aaataataaa aaaatacaaa tcaactatga caaaaggaat aatcttagca tctagaccaa
     7681 agatcgaaat ggtgaggttt ctttaaaata aaaatatcct tgcattttct cctagaaata
     7741 agtattttta aattacaaga ctattcttaa ggcgggtaaa aaaatgccaa tacgcgtaat
     7801 tcttatcgat gatgaacaga cgattttaga tggaatgaaa cagttgatca attggtctga
     7861 gcacgggttt caattaatag ggactttttc cagtccgctg aaagcgcttg atttttcttt
     7921 ggtacatgca atcgatatcg tggtcacaga tgtaatgatg cctgaattaa atggaatcga
     7981 attatcgcgg ctattaaaac agcaacaacc tgaaacacag atccttatcc tctctagcta
     8041 tgatgaattt gatatggtta aagattcttt taaagaaggc gtcgctgatt atcttttaaa
     8101 gcctcgattg agtcctgatt tttttctaaa ttccttaact gccgtttcac aaaaaatcaa
     8161 aaaaaagccc aacaaaagcc caatcaccaa tcgttatgag cagatctgcg agaatctttc
     8221 acagcagatt gctggacaaa ctcgtccatt gcttgaccct cttctttttg aacacacctt
     8281 gtttttctgt tatataccga aaaacggcca ttcagttcaa aacaaggctc taaacaaaaa
     8341 cagtcgatca cccattcatt gatggatagc gacgattcgt ttactattta tcctgtttct
     8401 accttttcgg aagaatccgg ctatgtaatc aatactgatt ccaaagatac acttaggaag
     8461 tttgttcaaa agtttcgtca agaagttttt ccaggtgatt tttttgttgt ttctgattct
     8521 tttgcaatcc aagatatcaa tgaaatattc caagagatca gaaattgcac caaaggacaa
     8581 cgtttctatc ataagggaaa gaaccttgct tatcagcatg aacttcttcc gttattaacc
     8641 aaccacgagc agcaagcaag tagttattta acaaaaatcg ttcataaaga ctacgcttta
     8701 gctgtcaaag aacttgagaa tttcttcacg acgatattgg aacagcctat tgatccagct
     8761 tatttgaaac atgaggcaat caatcgtttc tatgcattac tcaacgcttt ggctgacgag
     8821 catgttggga atgaagaact ttctttgctc aagatcgccc ttccttcttt attagccgat
     8881 gctgagtatt tcgaagattt ttctattctt gtcaaagaaa cacttgagaa actcacccac
     8941 ttttctgcaa atgcaaaaaa agacaatttc gatatcttga ctttgatcta cgagttcgtc
     9001 caggaaaatt atgagaaaga aatcaattta caacttttat ccgaaaaata tcatttttca
     9061 tatagctatc tctcagccat ctttactgaa aaatttggta tcaacttttc aaaatatcta
     9121 aaaaaagtcc gtatcagtaa agccaagctc tttttgacgg aaacacggct tactttgacg
     9181 gaaatctgtg agaaagtcgg ctataccgaa ctgggttatt tctccagagt ttttaaagaa
     9241 gagacaggaa tgacaccttc tcaataccgt aaaggaactt ttgtaaaatg agaaaaaaaa
     9301 tcaaagagca tgaactcttt gccaaacttc tgttcatttt gttcatcggt ctttttctgc
     9361 aagccatcgt catttctttg tttatttatc atcgatcaag ggatgcctat atccagctgt
     9421 tcaatcaatc gaatgatgtg gtactaaaaa agattcaatc tgaatttgaa agtttgaatg
     9481 atacaatcga gaatacatta gccgcttttg attctaatcc agctgtcaaa agctattttt
     9541 ccaatgatcc agcccaacat atggaacagc ttcagcaact aagaacaatc caaaaaatga
     9601 atgactctct ttcaaaaatc catccaatga tcgattacga tgtattgatc tttggagaaa
     9661 atggaagaac ttttgtcgga aacgacatgc tcactgccgt atctgccgat tctttttttc
     9721 aatctgcgat tgctcagaga gtgaatgaac gtgctgctga tacacaaatg ctttttgcac
     9781 accacggatt gactcttcgc gataagaaag cacccagcgt cttctttgtc aaaaaactga
     9841 aaaatacatt gaatcacgtt tacggatatg ctgttttatc gatctcatcc aagcaactag
     9901 cgaatctttt tcaatctgta gtaaatcctg agatatcgaa gatctccttc atgaacgaag
     9961 aacagacgat catcgcatca aatgaagaaa atacaattgg gagccgatca atgattttca
    10021 aaaaactaac agtaggtgaa acaacgattt caaatggaca gcagttgacc cgtcttcctc
    10081 tgtaccgcca ggattgtaca ttactcagtc aagtcgatct tcattcttta tctaaccaaa
    10141 tgggcgtgat ttttccgatt attttgtata atatcgtgac acttgctttt gctggctcat
    10201 tggtatttat gtatttggat cgacacacaa aatcgatcta ccgcttgatt gattcgctaa
    10261 aacggctaga ggaagatggc ttaaaagcta ctgttcctgt acaaggaagc catgaagtcc
    10321 aattgctagg gaatacgatc aatcaattgc taagtaatgc tcagcatcat tatgaacaga
    10381 cgatccaaga cgaaaagaag aaacgttcgc ttgaaatcca aactatgcaa gcacagatac
    10441 agccccattt tatttataat acgctgacta gcttgaaatt tttgatttgg cagaaaaaaa
    10501 cagatgaagc agttcaagga atcgacagtt tcattacttt actaagaagt acgattggga
    10561 aaaaagaaga agtgattcca gtcaaggaag aaatcaaaag cgtacaaagt tacatcgatc
    10621 ttttgagtat gcgatatggc gaaagaatct cggctaaaat aatgattcct gatgaactgc
    10681 agtcattatc tatgccgaat atgatccttc aaccaattat cgaaaacgcc tatcttcatg
    10741 cttttcacaa taaaacgcaa ggattcatca cggtgtacgg caaaacaaaa aatcagcttc
    10801 ttttattcga agtgattgat aacggcgatg gcttcgatac tgaaaatacc aaacaaaaaa
    10861 atgatcattt ttcaggaatc ggcatcagcc atgtagatga gcggatacgc ctaatgtatg
    10921 gcgatgcata tggcttaacg atccaatcag tgattgggac aggaacgact gtaacgattt
    10981 gtctaccgct tcttcctaaa aatcaatcag aatatcaaaa ataaagaata gccaagcgct
    11041 gattttttta ttttaagtta aataaatcaa tcagtattct tgactttcat ctacgcgtct
    11101 cttctactgt cagcactgga taacagccca atatttgact ttctcctccg agcatcttga
    11161 tttcttggag cgcattctct actaatgtca ttggacggtc caacaacaaa tcgatcacga
    11221 aaaaatattc tcccaagcta gttttcaatg gtcgggattc aatcttactt aagttgattt
    11281 cccgccagcc aaaagctgcc agcattttat gaagcatccc tggccgatta gcaggtagtg
    11341 tcaagatgac actcattttt actggagctg actgctggct ggtcatcttt cggtcaccga
    11401 cgatccaaaa acgtgtctgg ttcaactcat tatcttggat attctcagct aagatctcca
    11461 atccaacatg ctgtgctgtt tctaatgaag caatcgctgc tgcgtcttct ttcggatgtt
    11521 ctgctacata cattgctgca gcagttgttg attctgttgc tactaaagga acattaggat
    11581 aatgcgtttc taaaaactgt tgtgattgtg ctagtgcttg tggatgtgaa aggatttttg
    11641 taatctttgt tgctggattg cctaaaagct gctgcttgat aggaagaacg atttcacttt
    11701 taacttcgac ttctggatgt ttggaaagaa gatcgattgt gtgatgaaca gctccttcca
    11761 atgaattctc tacaggaacg acagccagat cgacttgttt gcgaaacaaa gcgcgcaaac
    11821 aaacagggat ggaagcatac gcagtcaatt gttctgtagg aaatagctga caagccgcct
    11881 gataagtgaa tgaactttct ggccctagat agctgacgtt catatgcttt tgatcctttc
    11941 tataatggta tcgatgatct tttctggtgt ttgattggtc gtgttgattc gatgtgtcgc
    12001 actttcttca taccaagaaa gtcgttgtgc caataatgct tttatttctt ctcttgtttt
    12061 tccaagagct aaaggtcgaa tagacgtttc atcttgttgg atacgatcaa ctagacagtc
    12121 cgcttctgcc tctaagtaaa gaacaatcgg ctgtgttttt aaaaaacgcc gattttctgc
    12181 actttggaca atccctccac cagtcgcgat aattttgtct tcatttgaca gctttcttaa
    12241 aatatctgac tccacttttc gaaagctttt ttcaccgtaa tagtgaaaat attcggcaat
    12301 cgacaattgg atatgttcct caatctttgt atctaaatct acatactctt tttgtaaaca
    12361 ttgagctaac cctttaccga ttgttgtttt tccagcgccc atgaaaccaa tcagcaaaat
    12421 gctagccatg agcttctcct cctgacacaa gttctgctaa atcgtcaaaa aatgccggat
    12481 aagagattga aacagcttca gctccttcta gttcgcaagg tccatcagct atcaaagcag
    12541 ctacttgcag catcataccg attcgatgat cgcctctgct ggacactttt gcgccatgca
    12601 gaggtgtcgg cccatggatg atcagaccgt ctgctgtcgc ttttatcttt gctcccattt
    12661 tctgtaattc ttctgccacc gcatcaattc tgttggtttc ttttactttt aattcttctg
    12721 cgtctttgat gatcgtgatt ccttctgctt gagtagccac taaagctaga atgggcaatt
    12781 catcaatcag acgcggaatg atttctccac caatcgttgc tttcttcaaa tgactcgtct
    12841 tgacacttaa atcagccgat tggttctgtt cattgtgatt cgtctgggta atcgctgcgc
    12901 ccatctcctc caacacatct aagataccag ttcttgttgg attgaccccg acatttttta
    12961 acaacagttg gctttccgga acaagaagtc ctgctgctag aaagaaagca gctgatgaaa
    13021 tatcacctgg aacagttatc tgttgaccag ttaatttttg cggtccggtc actatgatcg
    13081 ttttatcttc cactgtgatc cttccgccaa attgacgaat catttcttcc gtatggttac
    13141 gggaacgttc tttttcaacg attacggatg tgccttctgc ttgtaatgcc gcaaacagca
    13201 aagcagattt gacttgcgca ctggctacag gcatattgta tcggataggt gataaagagc
    13261 gggttcctgt cacagtgatt ggtagatact ggtcattttc ttctccttgc aagtcagcgc
    13321 ccatctcgcg taaaggttcc atcactcgcc ccattggacg tttagacaac gaagcatccc
    13381 cgaacagagt agtcgtaaaa ggctgtccag ccaaaatacc catcaataaa cgtatggtcg
    13441 taccggagtt tcccatgtca attgattcgg ttggttggac aaatgagtgg ctaccgcgac
    13501 catgaatctt gatcacctct tcctcttctt cgatctcgac acctaattgt tggaaggcat
    13561 gtaaagtact cagacaatct tctgcgcgca aaaaattttg tatagtcgtc gttccttcag
    13621 caattgctcc aaacatgatg ctgcgatggg aaattgattt atctgctggt atcctaacag
    13681 tccctcttaa tccatgtatt tgttgtaata attgcattta tccagcctcc tatttgatcc
    13741 agcaatgata actggtatta gacataatga ttctttttgc ttgtaacaaa tcctgttggt
    13801 ttttaaaact gatttgcagt attcctaaaa tatcttccct ggtctcttgg atcttcaaat
    13861 taatcaaaga aactcccgct tttccgatca gtccagttac ttctgcaata acgccaggtt
    13921 catctggcac atcgataaat aaatcaaaaa aggcaggaat cgcgccttgc ttatggacag
    13981 gcaactggtc tcgactttct tttgcccgat tgaaaaaaga aaagatttcc gattggttct
    14041 cagtcatgat ccattctgct atttgtgtca tttcttgttg ccaatctgcc aatcgttcca
    14101 ataatatctg cttattgctc aacaaaatat ctgtccacat gacaggatct gaggaagcga
    14161 tcctagtgat atctcgaaag cctcctgctg ccagttgttt ggctcgcgga tactcttgat
    14221 tgaattgttc tgcctgattg actaagccag ctgccagaat atgtggaaaa tgacttagca
    14281 ttcctgttat acggtcatgt tctcttgctg taagagttac atattttgct ttggttccgc
    14341 aaaatagttc ttgcagtttt tcaacatctt ttttcgtctt ttccaaaggt gtaaagatgt
    14401 aataggcatt ttcaaatagt ttagcatctg ctgcctgtat acctgattta tgagaaccag
    14461 ccattggatg tccaccgaca aaccgaagat ttttctgctc agcggatgtc acgacttgct
    14521 gcttcgtact gctgacatcc gtaatgatga cgtgatcaga caagttgatt ctttcaagga
    14581 catcaagata agctaaggtt gtacggacgg gaacggctaa taagataacg tctgcttgtt
    14641 ctgcacctgt ttcaaaatca cttgcaacgt aatcaacgat ctgctgttct actgcttgct
    14701 cacgggtttt tgactgacta tcccaaccaa ctactatcag ttctggattg gtctgacgaa
    14761 tacaaagtgc catagaagca ccaatcagtc caagccccac gataaaaaca tgttgtgcca
    14821 tcttatttgc ccccttttta gtaatgtttc aagtagtcac ggtactcctt cactgcagct
    14881 ttcaactcaa caagcgaatc acttgggaat tttgacaata gctctgtcgc taccacagtg
    14941 gctacaacat tttccgcaac gacgctagct gcaggaactg ctgtactatc cgaacgttct
    15001 acacttgctt tgtaaggttc ttttgtatcg atatcaacac tttgaagtgg cttgtacaat
    15061 gtaggaatgg gtttcatgac cccgcgaatg acaagtggtt cgccattggt cattccgcct
    15121 tcgaagccgc ctagattatt ggtacgtcgg aaatagcctc tttcttcttc ccataggatc
    15181 tcatccatca cttctgagcc tggtaatgaa ccagaagtga atccgacccc aaattctacg
    15241 cctttaaagg cattgatact aaccacagct tgtgcgattt tggcatctag cttcgtgtcc
    15301 cattgtacgt agcttcctaa tccagctgga acgcctccaa cgagcacttc tacaattcct
    15361 ccgatcgtat ctccattttt ctttgtctga tcgatcaaat cttttattct ttgctcaact
    15421 gctggatcaa caactcgtac ttctgacgct tcagaaattt ctttgatttc ttgaatcgag
    15481 atatttccag gtatttctgc ttggattcca ccaagacaaa tcacgtgtcc tgctacgatg
    15541 atgtcaagtt cttccagaat tttcttggca attgcgccga ttgctacacg catggtcgtc
    15601 tctcttgcag aggaccgttc aagcacattc cgaagatctc gatgatggta tttcataccg
    15661 cctaccaaat ccgcgtgtcc tggacgcgga cgtgcaactc gccgcagtga ttggtctttt
    15721 tcaggcactt ctgctacaga catgacagtt gtccaatttt tccaatcttt attttctacc
    15781 actaaagtga caggtgaacc aattgttttg ccgtgacgga tgccagacgt gatccgaaca
    15841 cgatcttttt cgatcttcat ccttcctcct ctgccgtaac cagcttgtct gcgagcgagt
    15901 tcgtgattga tttcttcttc tgacagaggt aatcctgctg gtagtccttc aataatggct
    15961 gttaattctg gtccatggga ttctcccgca gtcatataac gcatcttaca gtcctcctaa
    16021 gtaatctttg aatgtctctg tgggaacagg aacgattttt gcttgcccaa tcttttctag
    16081 caagatgata ttcaattggc ttcctcttgc tttcttatca tgggttatcg cgtggaataa
    16141 ttcatctgga tgacttagat cgtacgtaat cggcagatgg aatttttgaa gtatcctttc
    16201 taaagcagct gtcgatccag ttggagacaa gccgattttt tctgcatgag aagtgatggc
    16261 taccattcca atagctactc cttctccatg actgactacc ccatatcctg ctgtattttc
    16321 aattgcatgg ccaattgtat gaccaaaatt taaaataagt cgatttcctt ggtcaaagac
    16381 atcttcttcc actacttttc gtttgatttc caacgtagca gtaatgattg gaacagcatg
    16441 ttccagaaga tctttttcgt ctttcagctc gttcagctgt ttccaaagcg ttgcatctgc
    16501 tatagctgct gatttgatga tttcagcgat tccttctcgt aaacgacgta ccggcaaagt
    16561 ttttagaacc tcaggatcaa tcaatacacc agctggctgg ctaaatgttc cgacaaggtt
    16621 tttcgctttg tttgtattga ctgctgtctt cccgccaata ctagaatcta cttgtgcaag
    16681 tagcgtagtc ggaatttgaa cgaaatggat cccacgcatg aacgtcgagg ccacgaatgc
    16741 tgctagatca cccaccattc ctccaccaag tgcaagtatc ccatcgctgc gggtaaaatc
    16801 attatctgct aaaaaatcgt ataactcagc tgcttgttct aaagatttac tggtctcacc
    16861 cgccggtacg attgaaagga ctggttcaaa attagcttgt ctcaattgga acatgatttt
    16921 ttctgcatat agtggagcaa cgtttgtatc agtgatcaca gctacttttt gaggtctcca
    16981 aatcgtttga agccatgatc cagcctgttc tagtgcgcct ttttcaatat gaatatcgta
    17041 tttttggtta tcaacatgta cagtaagcat tcgtttcact tctttctttt aaaaagctgg
    17101 aattaatcga gtgtctgaat cttttccatt gcttctttca tgatcgcaac ttctttcatc
    17161 atgcgtgagt aagtcttttc attcaacgat tggggaccgt ctgaccaagc atttgcagga
    17221 tcaggatgga tctctacgat catgccatct gctccgcttg ccacacccgc acgagccatt
    17281 ggtggtacaa gttcccaaat tcctgtacca tgacttggat caacaatcac agggaaatga
    17341 gttaattttt tcatcaatgg gacagcactt aaatcaaacg tattacgtgt cgctgtttcg
    17401 taagtccgaa tcccacgttc gataaaaatg acaggagatt tgtcttcgac tgcaatgtat
    17461 tctgcagcat taagccattc attgatcgta ccggaaattc cacgtttcaa tccaacaggt
    17521 ttacctgttt taccgacagc agatagcagt ttaaaatttt gcatattacg tgcgccaatc
    17581 tgtaggatat cgctgtactc agccaccata tcgatatgcc cttcatccat cacttcagta
    17641 atcactttca ttccaaattc atcagccgct tgacggatgt atttcaatcc ttcttcttct
    17701 aaaccttgga aagcatatgg agaagtacgt ggtttgaagg caccacctcg cagaattttt
    17761 gcgcccccgg ctttggccat tcgcgcacat tctctgattt ggtctaatcc ttcgatagaa
    17821 caaggaccag ccatcgtcac gaaactgccg tcaccaattt ttactccgtc tacatccacg
    17881 actgtattgc caggatggaa ttctcgagaa gtcaatttgt atgtgtttaa aatacgcaca
    17941 gcattttcta ctccttcata gcgtgtaaat gctccttctt gtaaacttcg cgtatcacct
    18001 tttagtccaa tcactactcg ttctgttcct tgatcgattg agactttgaa tccttctttt
    18061 tcgatatgat cgacgacttt ttttatttgt tcttccgttg cttctttttt cataattact
    18121 atcattgttt ttcccgcttt cttttgttta cttatctatc ttggctgatt ttctctactt
    18181 tttcaaggtg gttttacatt aattcctact aaatgaccgt gtcctgttgt tctcattttc
    18241 gctccttctt tcctacttat gacccagata atcaacaaaa aaagatgtcc ctgtaggaaa
    18301 ttgcctctac agtgaacatc ttcaacgagc cttaaaaagc gctgcaaaaa tataaaatag
    18361 ccactataga tgaatttctc tacgtggctg taagtcgtgt ttaaaaatct ttagccatca
    18421 tagatacaaa cctggtttgt ttgtacccac gaagtcatga atacaaacct atctaaaata
    18481 gctaaagtaa tacgtattca gttttgtaag ttttctagat ttcataaaaa atcgctccaa
    18541 ac
//
LOCUS       DBKOIJ010000046        18504 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000046 DBKOIJ010000000
VERSION     DBKOIJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.046"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            99..746
                     /locus_tag="ACV5NJ_001232"
     CDS             99..746
                     /locus_tag="ACV5NJ_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HHY6485333.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACV5NJ_001233"
     CDS             1139..3784
                     /locus_tag="ACV5NJ_001233"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HHY6485334.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACV5NJ_001234"
     CDS             4094..5407
                     /locus_tag="ACV5NJ_001234"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HHY6485335.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACV5NJ_001235"
     CDS             5397..6050
                     /locus_tag="ACV5NJ_001235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6485336.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACV5NJ_001236"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACV5NJ_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HHY6485337.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACV5NJ_001237"
     CDS             6947..7147
                     /locus_tag="ACV5NJ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HHY6485338.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /gene="rnjA"
                     /locus_tag="ACV5NJ_001238"
     CDS             complement(7401..9080)
                     /gene="rnjA"
                     /locus_tag="ACV5NJ_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J1"
                     /protein_id="HHY6485339.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACV5NJ_001239"
     CDS             complement(9082..9294)
                     /locus_tag="ACV5NJ_001239"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HHY6485340.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACV5NJ_001240"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACV5NJ_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HHY6485341.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACV5NJ_001241"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACV5NJ_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HHY6485342.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACV5NJ_001242"
     CDS             13269..13466
                     /locus_tag="ACV5NJ_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485343.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACV5NJ_001243"
     CDS             13619..14104
                     /locus_tag="ACV5NJ_001243"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HHY6485344.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACV5NJ_001244"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACV5NJ_001244"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HHY6485345.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACV5NJ_001245"
     CDS             14981..15508
                     /locus_tag="ACV5NJ_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485346.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACV5NJ_001246"
     CDS             15599..16336
                     /locus_tag="ACV5NJ_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HHY6485347.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACV5NJ_001247"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACV5NJ_001247"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HHY6485348.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACV5NJ_001248"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACV5NJ_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HHY6485349.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DBKOIJ010000047        17770 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000047 DBKOIJ010000000
VERSION     DBKOIJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.047"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            44..421
                     /locus_tag="ACV5NJ_001249"
     CDS             44..421
                     /locus_tag="ACV5NJ_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485350.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            990..2378
                     /gene="ltrA"
                     /locus_tag="ACV5NJ_001250"
     CDS             990..2378
                     /gene="ltrA"
                     /locus_tag="ACV5NJ_001250"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HHY6485351.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(2500..3795)
                     /gene="purB"
                     /locus_tag="ACV5NJ_001251"
     CDS             complement(2500..3795)
                     /gene="purB"
                     /locus_tag="ACV5NJ_001251"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HHY6485352.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3808..4932)
                     /gene="purK"
                     /locus_tag="ACV5NJ_001252"
     CDS             complement(3808..4932)
                     /gene="purK"
                     /locus_tag="ACV5NJ_001252"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HHY6485353.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(4925..5413)
                     /gene="purE"
                     /locus_tag="ACV5NJ_001253"
     CDS             complement(4925..5413)
                     /gene="purE"
                     /locus_tag="ACV5NJ_001253"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HHY6485354.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(5829..7136)
                     /locus_tag="ACV5NJ_001254"
     CDS             complement(5829..7136)
                     /locus_tag="ACV5NJ_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HHY6485355.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(7139..7720)
                     /locus_tag="ACV5NJ_001255"
     CDS             complement(7139..7720)
                     /locus_tag="ACV5NJ_001255"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HHY6485356.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(7836..7933)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(8292..9584)
                     /locus_tag="ACV5NJ_001256"
     CDS             complement(8292..9584)
                     /locus_tag="ACV5NJ_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHY6485357.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(9581..10204)
                     /locus_tag="ACV5NJ_001257"
     CDS             complement(9581..10204)
                     /locus_tag="ACV5NJ_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485358.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(10207..11043)
                     /locus_tag="ACV5NJ_001258"
     CDS             complement(10207..11043)
                     /locus_tag="ACV5NJ_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485359.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(11060..11947)
                     /locus_tag="ACV5NJ_001259"
     CDS             complement(11060..11947)
                     /locus_tag="ACV5NJ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485360.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(11963..13246)
                     /locus_tag="ACV5NJ_001260"
     CDS             complement(11963..13246)
                     /locus_tag="ACV5NJ_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6485361.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(13929..14612)
                     /locus_tag="ACV5NJ_001261"
     CDS             complement(13929..14612)
                     /locus_tag="ACV5NJ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HHY6485362.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(14630..15247)
                     /locus_tag="ACV5NJ_001262"
     CDS             complement(14630..15247)
                     /locus_tag="ACV5NJ_001262"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HHY6485363.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(15257..16081)
                     /locus_tag="ACV5NJ_001263"
     CDS             complement(15257..16081)
                     /locus_tag="ACV5NJ_001263"
                     /EC_number="3.4.-.-"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HHY6485364.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(16074..16634)
                     /locus_tag="ACV5NJ_001264"
     CDS             complement(16074..16634)
                     /locus_tag="ACV5NJ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485365.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16808..17764
                     /locus_tag="ACV5NJ_001265"
     CDS             16808..17764
                     /locus_tag="ACV5NJ_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHY6485366.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         5343 a         3873 c         3177 g         5377 t
ORIGIN      
        1 ttaactaaaa atttggataa tgggttcgag gaggcacttg ccgatggaag atgcaataaa
       61 acaaggtcag ctttttttat cagaattaac agctgaagaa cgttcctgtc tatataatga
      121 tttgctgtcg atcaaagacg tttatttatg caataaagat actagttttt atacattccg
      181 aaaaatccgc cgagcaacca agcgctgtgc tgaatgccgt aaaaggactt ttctttatca
      241 gacagaacac aaaggcgaac tttcaatcag tcagattcat gaacagaagc gtttcaaaaa
      301 tcattatcgt atccagatct tattcaatgg acaagagatg gaatacgctt ttctcgaaaa
      361 cttgctgcaa catctccata accagcattt tagttataat agccaagcca tttacgctta
      421 attcctctat tttttgtatg taaagaggca cggtaaagag gtagccgctt ttaaaatcag
      481 gttcctatta ttgataagaa aatataataa actaaaagaa gcagaaccgc taacggatct
      541 gcttcttttt tggtgtgcgc cctgcatggg tgaaaacttg gtggtgaaag tccactacag
      601 gcttggcagt aggaactgtt agcgaaagac aaggcggcaa tcgtgaggtt gtggctgaag
      661 gaagtcggac gcaaactcct gaactgacga acagaaacta gatacaaggc tgaattaggt
      721 cggataagtc ttcatcacaa gacaaagtcc aatactgccc gaaacctata cagtaaatct
      781 agcagttaca tgggaggaaa gttttcactc ttaccggggg agatctcatg agggtgttgt
      841 gggcgttgaa tgaaaacaaa ccagcacaac gatcatggcg acatgatggt gaatcatgag
      901 aagtcagccg aggtcatagt agtaacgaca ctgaccgtta cgaaggactg aacaataaga
      961 atcttgaata atttaggagg tgtgagaaga tgcgacgacc gcagaaaaca agaaaacttg
     1021 gctatcatca aagagctagt gtggaacacg aagggtatga tgaagtgcgc agcacttctt
     1081 gcggtgaaca gaggaaacaa gatggtgtag aaaatctgtt tgggagaatt ctagatcgga
     1141 ataatctcaa tcaagcgtat cttcaagtag tcagaaataa aggggcggct ggtgcagatg
     1201 ggatgaccgt cgatcaactg cttccttact tgaaagagca ccgcgaggaa ttactatcca
     1261 atttacgcca agggaactat aaacctcagc ctgttttgcg agtagaaata ccgaaaccaa
     1321 acggcggtgt aagaaaacta ggaattccca cggttattga tcgtatgctt caacaggcaa
     1381 tcaatcaagt gttacaaccg ctatttgaac cagaattctc agataatagt tatgggtttc
     1441 gcccaaatcg cagcgcacat gacgcagtga agaaagcaaa atcttattat gaacaaggtt
     1501 accgatatgt ggtggacatt gatatgaaag cctactttga caccgtaaat cacgataagc
     1561 tgatgtattt cattgagcgg aaagtaaagg acaaacaagt cctaaaactg atccgtcaat
     1621 acttaatgag tggtgtaatg gaaaatggac tcatcacaac aactgaagaa ggaacgcccc
     1681 aaggaggcaa tctctctccc ctgttaagta acatctacct gaacgaattt gatcaagtgc
     1741 tagaaaatct aggacataaa tttgttcgat acgccgatga ctgcaatatc tatgttagaa
     1801 gcaaacgagc aggacagcgt gtgatgaaga aatccattca atttctcgaa gaacaactaa
     1861 agttgacggt aaatcgagaa aagagtgcgg taggaagtcc attgaaacga aagtttcttg
     1921 gattctgcat tctgccaaca aagaaaggta ttaaaattcg ccctcacgca aaagcaaaac
     1981 agcgtgtgaa aagcaagctc aaacaactca ctaaaagaaa ccgtggaaga agtatccaac
     2041 ttattttaaa agaaatcaag caactaatga ctggttggat caactactat ggaattggtg
     2101 agatgaaaga attcatgagg gagttaaacg gttggttgaa gcgaagaatc agacaatata
     2161 tttggaaaca atggaagaat ccaagaacca aaagaaaaaa tctgattcgt ttagggatcg
     2221 ataaagcgaa agcgtatgaa tggtcaaata caagaaaagg tatttggtca gtctccaaaa
     2281 gtcatattct tcatcgatca ttaacagaca aagaactggc ccgccaagga tatgaggaca
     2341 tctccttgaa gtaccagttc gtacactcaa cttattgaac cgccgtatac gggtccgtac
     2401 gtacggtggt gtgagagggg cggaaggtca gttggctttc ccctctactc gattattttt
     2461 gctattctat tttaattgaa ttttgcaact cactcttctt tattccaaac ctactcgttt
     2521 gaatatcacg tcaacgtttt tcaaatgatg atgataatca aaggcatcgt ctatttcttc
     2581 tttagataat accgatgtga tggttggatc ttcttctaac agcgggcgga actgcacttg
     2641 gttgtcccaa gcgtacgcag tttttggctg aacaagatca taagcttctt ctcttgtcat
     2701 tcccttatcg atcaatttca gcataacgcg ctggctataa atcaaaccaa atgttgcaga
     2761 catattccgc ttcatgtttt caggaaagac cgttagattt ttaacgatat tgccaaaacg
     2821 atttaacatg taattcaata aaatcgtggt atcaggcaag atgattcgtt ctgctgaaga
     2881 atgagaaata tctctttcat gccataatgt gacattttca taggcagtga ccatgtgtcc
     2941 gcgcaccact ctggctagac cagccatatt ttctgaaccg atcggattcc ttttgtgcgg
     3001 catagcagaa gaaccttttt gacctttcgc aaaaaattct tctacttcac gtgtttcgga
     3061 tttttgcaat ccgcgtatct ctgttgcaaa acgttcaata cttgtagcaa taagcgccat
     3121 cgctgagaaa tattcagcat gtagatcccg agggagaact tgagtagaga tttcttgtgg
     3181 acggatcccc aattgtttac atacatactc ttcaataaat ggcgggatat ttgcgaacgt
     3241 tcccactgct cctgagattt ttcctgcttc tactcctttt gccgcatgtt caaatcgttc
     3301 gatgttccgt ttcatttctg aataccatgt ggcaagtttc aatccaaaag tagttggttc
     3361 agcatggaca ccgtgtgtcc gacccatcat cactgtatat ttgtgttctt ttgctttttc
     3421 gccaatgatt tccgtaaaac gtttcaagtc ttcccgtaaa atatcatttg cttgcttgat
     3481 gagatagcca taagcagtat ctacgacatc cgtacttgtt agaccataat ggacccattt
     3541 tctttcttct cccaatgttt cagaaaccgc tcgtgtaaac gcaacgacat catggcgtgt
     3601 taatttttcg atttccaaga tccgatcgat atcaaatgat gcatgctcac gaattttttt
     3661 cacatcttct tgcgggattt caccaagttc tgcccaagct tcatctgcta aaatctcgac
     3721 ttcgagccac gcacggtatt tgttctcttc cgtccagata ttccccattt cagggcgtgt
     3781 ataacgttct aacatgtttt ttctcctcta atcccaaata ttcgtttgtt tgatttcttc
     3841 cagcgtttgc tggatgtctt ctgtcaagat agtgatatgt cccatcttgc gacctttttt
     3901 tgcttgtttt tttccataga aatggaattg ccagtctggc ttattctcaa tcaagcgata
     3961 gctttccagc agttgttctc ccaaaacatt gaccattact gcattgctta tcaaacgtac
     4021 tttttctcct aatggccaac cacaaatacc tcgaatatgg gcatcgaact gactcataga
     4081 acaagcttca atagaataat gtccagaatt gtgaggacga ggagctaatt cattcacata
     4141 tagcccgcct gtttttgtta aaaacatttc tacccctaaa acacctgaaa ggctcactgc
     4201 ttctgcaatc acacgggcaa tacgctcagc ctcctcgatc acatcctgat caatcgcggc
     4261 tggagcaatc gtttcatgaa gaatattgtt gtggtggatg ttttccacga ccggaaaagt
     4321 cgtgaaatct ccttgaccat ttcctgccac catgatcgaa atctcttttt caaacgggat
     4381 ccatgcttct aaaacacagg ttccttctct taaaaggtcc atggcaggag ctagatccgc
     4441 ccgacttttc aaaacatact gccccttgcc atcatagccg cctcgagtgg ttttcaaaac
     4501 acaaggatag ccgatcccgt caatcgcatc ttggatatcc gtcggactga cgatcgttgc
     4561 ataaggagca atcacgatgt tattcgtttc caaaaaagat ttttctaaca ggcggtcttg
     4621 cgtaatcgca agcaagtctg tcccttgagg aataaatgac atgggtaaaa tcgcatttaa
     4681 cgcctctaca ctgacgtttt cgaattcata ggtaatgaca tctgttcttc tggcaagctc
     4741 tttcagcgca aaggtgtcat catagacact ttcaatgtgc caatctgcaa cttgtgcggc
     4801 tggacaatcg ctcacaggat ccagcacacc tactttaaag cccatttctt ttgcactaat
     4861 cgccatcata cgacctaatt gaccgccacc gacgatcccg atcgttgaac caggcaacaa
     4921 aggtttaacc aagttgatca ctactttcta taacagtttt agccaacatt gaacgtcttt
     4981 cagccaattt agcctccaat tctaaatcat acatggaaag catctgtatc gcaagtagtc
     5041 cagcattaat tgctcccgct tttccgattg ctgtagtagc tacaggtact cctccaggca
     5101 tttggacaat cgaaagaagc gaatccaaac catttaatgt tcgtgattgg accggtacac
     5161 cgataaccgg caaagttgtt tttgatgcga ccatacccgg cagatgcgcg gctccccctg
     5221 ctcctgcaat aatgattttg attccttttt ttcgagcgtt ttgtgcatat tcaaacatca
     5281 aatctggggt tcgatgagca gaaacgacct ttttttcata agacacaccg aattcatcca
     5341 atatttgaca tgcttctttc attgtttccc aatctgaagt actccccata atcacagaga
     5401 tttttgctgt cattgttttt cctcccagtt tttctacatt catattttaa caatcacttt
     5461 ttttgatgtc aaagaaattt cgaatgttaa caaagaaaaa cattttattg ttcgtgttta
     5521 ttaaagataa aaaaaaggaa tgtgacaaaa gttaggtcac attccttttt atggataaac
     5581 ggtgttctcc tgccaactcc tcggtcacaa ggaacaatat tcaacaaaca tgagaagctt
     5641 ttttttgaaa ttgttccttg tgactcggag atgaacggca ggctcacgac ctcttccttc
     5701 aacggtgttc aatcagctga ccctcggtat tagctcaaac aaacggcaaa atacgaggaa
     5761 ctatttttcc gtttgtttct ctaacaatca ggtcaaaacg ctgatttcac aacctttctt
     5821 ttgctctatt acttcttgaa ggcttcttcc aatatttctt catctttttt caatccatta
     5881 aagatcaaat tcaatatgat agccgtaatg ctactcatta cgatgccatt tccagtgaac
     5941 atttgcaatg tttgcggcat ttgactgaat aatgtcggcg taacattgaa acccagccca
     6001 aatccaacag aaacagcaac aatcaataga tttttatcat tgctgaaatc aacttccaac
     6061 agcatacgga ttccttggac tgccaccatc ccgaacatga ctaacattcc tccaccaagt
     6121 accggctcag ggatgatttg tgctaacgca ccgattttag ggaataatcc taaaacaatc
     6181 aagaagaatg cagagaaata gatgggtttt cttgttttga ttcccgaaag ttgaaccaat
     6241 ccgacatttt gagaaaagcc agtataaggg aatgtattga aaataccgcc taagataacg
     6301 gcaagtcctt ctgcacgata tccctttttc aattcttctt ctccaacatt tttcccagta
     6361 atgtcaccaa gtgcaaaata tacacctgtt gattctacca tgctgaccat ggaaatgatg
     6421 atcattagta aaatcgacca tacgtcgaaa gttggtttac caaaataaaa gggttgtgga
     6481 acatggaaca acggcgcatc tccaatcgcg ctcagatcaa tcattcccat gaaagcagct
     6541 aagatgcttc ctccgactaa accaatcaat accgcgattg aacgaatgaa cccttttgcc
     6601 agcatttgca cgcctacaat taggagaatc gtcacaaatg caagtagcag atttgttttg
     6661 tcaccaaaat cagctgctgt tgcattacct ccacccattt tctcgattgc tacaggaatc
     6721 aaagtcaaac caataacggt gataaccgtt cctgttacta taggagggaa tagtttttta
     6781 attttagaaa agacaccaga aactaacaca acaaagatcc cagaagcgat aatagatccg
     6841 tagatagctc ctactccttc attggttccg atcaagatca gtggagctac tgcttggatc
     6901 gcacatccta aaacgacagg caagccaatt ccgaaaaatc ggttaactgt caattgcaat
     6961 aatgtcgcta atccacacat aaagatatca attgaaatca aataagtcat ctgttcttga
     7021 ttaaagccta gtcctgtacc gatcaaaaga ggcaccgcta ctgctcctgc atacatcgct
     7081 agcagatgct gcagccctaa aaccgcagct tttccatttt gtgtttcttt ttccacgatt
     7141 atgcgtcctc ctcaagaaac tcaacttgtc cattcgtcaa ggaagcaata cgcgcaagag
     7201 aaacgactct caatcccatt tcttcaagta actgacgtcc atcttggaaa gacttttcga
     7261 tgacgatccc gattccttct acttgcgctc cagcttgctg gcataattcg atcaatcctt
     7321 ttgccgcttg tccgtttgcc aagaaatcgt cgataatcaa tactttgtct tctgaagata
     7381 aaaatttacg agaaatagaa atcgtacttg tcacttgttt cgtaaaagaa taaacagaag
     7441 ctgtcaataa ttcttcatcc atcgtcaaac tttttgcttt tctagcgaag atcattggaa
     7501 catctaattc tcttgcagca aataaagctg gtgcgatccc agaagcttca atggtcacaa
     7561 ctttcgtgat tccttgttct ttaaatactt ctgcaaaccg ttttcccatt tgttccatca
     7621 atacagggtc tacttggtga gtgacgaagc tgtcgacttt taatacgccc tcgcctaata
     7681 tacgtccgtc tttttgaata cgttcaacta attctttcac tttatttgct cctttatcat
     7741 ttattaatac cgaaataacc gcttttgtgc aaaaacctac tatgtttctg ttaaagaaaa
     7801 acaccctcca ctttatcata agaaaagaag ggtgaaatag ccaatctttt acttatagtc
     7861 cagcatttac ggtactggct agaaactgtc atccatatta tgacgatata taagtaggta
     7921 tgtaaaacta ataatagtgt aaattcattt tcttgcaact gttttgcacg tgaaggcagt
     7981 ttgccatatt tgaataaaaa atgttgtttc aaatattgaa aacagtctaa cactactttc
     8041 tgaaactgac aacgtggttt acttattttt ttgtaaaatc taatgatttt taatgcatta
     8101 ttcctttttg ttcgggttta aatagttatt tttattttct cctatctaaa aaagcagccc
     8161 acataaaaag aaggcatgac aaaaagcatg tcatagcccc tttttccgaa taaatagtgt
     8221 tcaaaagaca gcctatcggc aataaggcaa gattttataa actacgggaa gtaatttccg
     8281 taaatctatt cttattcttt taagctgaca agctcgtcaa tcactactgc ttttcctgaa
     8341 gcaatagaat gatttgctgc gattcctgtc aaaatcgaca aaataccatc tacgtgagtt
     8401 gctgcccgat tccacggatc ttcttctttt tcttcaccaa atagatcatc taataaacgc
     8461 ggatctcctc cgccatgtcc gcccgcttct tgttcgacga ttacctcata tggctcaccg
     8521 aacatcggat agacatggat ggactgaaga acggctgctc cttcatcctt tttatctcca
     8581 cctgcattga tgtaagactt ttcgatcact ttcatttcga tccgtccttt gctgccattg
     8641 aaagaaacat tgaatccttc ccacggcata tacgcgttta gcgaatacgt caaaatcgca
     8701 ccagtttcat atttgaccgt tacgcctagt gtatcttcaa tagagatatc atcggaaaat
     8761 acactgcggt cacgaatata tccactgtct ttttctgctt ctaaatacat ttctcgcaat
     8821 tcctcattgg ctgacaggtc tatagcaaat ggatcagctt tagctagttc gcttccatag
     8881 catcgactgt aaaatatttc tactccgcgt ttctccgcat tatgcatccc ataaaagttt
     8941 aatccaccga atgcaaatac cttttgggga cggctgttca accaaaaatt cactagatca
     9001 aaatgatggg tagatttatg aacaagaagc ccgccgctat tgtgcttatt tcgatgccat
     9061 cttctgaaat aatctgcccc gtgttgcgta tttaacagcc attcgaagtg gatagaaaac
     9121 acatcaccaa tcacatcact ttctatcagt tcctttatct tcgtgtgata cggtgcataa
     9181 cgataattaa aagtgactcg caggcttctt cccgtctgtt tttggaccgt tattatttct
     9241 tgagcttttt cctcatcgat agtcagaggt ttttcgcaaa taacatcata tccttttttc
     9301 attgcacgaa tgatataatc atgatgcgtt cgatctactg acgtaataat gacttcagcc
     9361 ggccgtacgt catccagtag ttgctcaaat tgtgatggca tacatgtctt tacttttttc
     9421 tgtccatact tgttcacaag tatccgattt gcataatcca tccttgtttg agatacatca
     9481 caaaaaccga ctagttcaca agtttgatga tacgtagagg caattgcctc ataaaaaaaa
     9541 cgcgctctcc caccaagtcc gacttgtaca tattttttct tcatttaaag tcattctcct
     9601 tttcttttaa gcaaaatact gtgacaaatg gcagccctgg atacagtaat gcagtgagta
     9661 aaaaatcatg ctgtatacac cagaacagtc catacaaaag gcataaatac cacagaaacc
     9721 ccttgacgtt ttttgacaac agccagatat tccttttgat caaataaaca agactatctg
     9781 ctttatcttc tttgcagata tcaattagcg tactaaaaaa actatgggat acaaaaagca
     9841 acagacacaa tagaaaagga atcagatacc aagctccaat ctttcccact aataaatacc
     9901 aatcaactgc aaaaataatg acaaacagct gaaacaacgt aatgatgaaa cacatccgtt
     9961 tgatttttcc tttcctccaa catacctttt tttctgccaa gtaatggaca gatagataat
    10021 aagaaggata catcatcaaa aaataaatca gatagacaac acgagcagac agcactaatc
    10081 ctaaagtgaa caaacatcct agaactaaag cgaaaagagc attactgata agactggtta
    10141 aaaaaatccg acagacatag tagttgagct tgtaaagaaa catctcttct attggctttt
    10201 tcatattcag cccttcagac cgcttgtact gatcccctgg acaagatatt tattgaagaa
    10261 taagaaaatc agaaaaaccg gtagcagcga caacgtagac atcgcaaaca tactcgcgta
    10321 agtcgtacta gatccagcgt ctgcaaataa tttgatggca atcgataccg tgtaagatct
    10381 cggcttgttt aaataaatca atggtcccat gaagtcgtcc catttccaat aaaactggat
    10441 gatgatcgtc gtgatgatcg aaggtttcag caaaggaaaa ataattttgg taaagacact
    10501 atacttgctg catccgtcaa taatcgctga ttcatctaac tctttgggga tgctgaccat
    10561 gaattgcatg atctggtaaa tgaaaaatgc ttgtccaaaa aaatgtggta aaatcagcgg
    10621 cacatacgtc ccaaccaaat tcagccgatt gtaaataata aactgcggaa tcaaaatcac
    10681 ttgtgcagga atcatcatcg ttactaacat gacgacgaac cagaatcttt ttcctttgaa
    10741 atcgattctc gataatgcgt aagcaataca agtcgagctc agaactgtcc ctatcgtcga
    10801 aactgcagag atgacaaacg aattcctaaa gaaagtaaaa aatgaaattc cagcaaaccc
    10861 cgcccatcct tttgcgtaat tttcccattg aaaagtctct ggaatcaaat tcagcgaacc
    10921 actcataatc tcttgatccg ttttaaaaga acctgcgatc atccataaga ccggataaat
    10981 catgactaga cctaatagca aaataaaacc gtattgaaac catttcgtta ctgtattatt
    11041 catttcttcc tcctctcatt cacacaacga cccatttttt tgatgatttg aaaatcagaa
    11101 aggtgacagc tcccataatc aacagcatga tccacgaaat agcactagca taccccatag
    11161 aattaaaaga aaaagcttgg ttatacacat aaagagcata aaagttcgtg gcatcattcg
    11221 gaccaccttg ggtaatgata aatgcctgcg taaaagtcat aaacgccgcg attgtctgca
    11281 taaccaagtt atacaaaatg atcggcgaaa gaagaggtaa cgtgatcttg taaaacgtct
    11341 gccatttgtt cgctccatcg atctctgccg cttcataata cgagcctggg atctctttga
    11401 tacctgcagc aaagatgatc attgccgaac cgaattgcca agcagacatc aaaacaagag
    11461 ggatcatgac cagtttttga tcgctaaacc aggaaagcgc aggtgcgcct aatcgttcca
    11521 ggaaaatatt cactaacccg tttttagaaa acaactcccg ccaaactaga gcgatagaaa
    11581 tactgccacc gatcaaacta ggcacataat acaaagaacg aaagaaagtc gttcctttga
    11641 tattcctcgt taaaagataa gcaatcaata gaccaaagat cagtttcaca ggtacagatg
    11701 caaatacata gagaaaagtg actcggatgg agttcaaaaa acgggcatca ctcagcatct
    11761 ttatataatt atccaagccg atccatgaga ctggatttcc caaacgataa tcggtgaacg
    11821 aataataaag tgacaagatc atcggtatca ctgttatcaa tagaaatcct aggatgaatg
    11881 gagaagcaaa tacgtaaccg accacattgt cttgatccaa gaaccgtttt agtttttctt
    11941 ttttcatgcc ttcctcctgc tgctatccta gaatgtttga cgcaaactca taaagactct
    12001 ctgctgcttc ttctgctgtt acttcttcat aaatgacttt ttcaactaaa agattgtatt
    12061 ggtcttgaat ttccggattc aaagcactat caaagacatt cacttcgccg ttgtccatat
    12121 cggaaatcat ttctaaataa tcaaatgtcg cttgcaaagt tgcatcatct gcacgagcga
    12181 gctgttctcg aatattcgac atgattggta caccacgttc tccttttaaa atttcattgg
    12241 cttcttcact gttttgaaag aaatcaataa acttagcagc ttcttcttta tgggctgagc
    12301 tttttgccat actcagcatt tgcgaagaac gtaaagggat accagattct ccatctgctt
    12361 ttctgcgagg tggattaatc aactgaatct ccatatcttc aggagcccct gacaatattt
    12421 ctgtcaactg gttacttgat agaaacacca ttgccgcttc tctatcccta accgcctgaa
    12481 aagacagatc cgtcgtactt gcagctccac cacctggttc tggatgggcc cctttgtcta
    12541 ccagattttt acgcagctgg aaaaaatctg ttagatagtc tggattttca aatcccaagc
    12601 tgttagatag atcttcccga ttataaaaat tcaatccttt ctcaacttgt ggtatttgaa
    12661 taatacagcc agctataaaa tcttcgaatc ttgaaatagc atagattcct aattcttcag
    12721 tgatttgata gctgatttgt tcaaattcat cccatgtcca atttgaagct ggttcaggga
    12781 tatttaattg ttcaaatata tttttattgt aagcaatcgc aaacgaattc accccgttag
    12841 acagtccaac taattgcccg tcgtaagtcg tcgttgctaa ataggattca tctgcattag
    12901 aaatatcaat tatcccttgc tccacatatt catctaatgg ttcaatctga ttgatgtatt
    12961 gaggaaattg attgttcagt tggaaaatgt cccagatatc atctgatgct gctagtgtac
    13021 ctaattttgt gtaatatcct tcataatcat aaaattcata atcgatcgtc acatggggat
    13081 ttttttcttc ataaagctcg attactttta ctgttgcatc gtgtcggact tgcgaacccc
    13141 accaagccat gttcagcgtg atttttttgt catcttcttg cccacaagcc gtcaagaaga
    13201 ataacataac acttaaagct aagatgattc tcattctttt cttcattctc tgcacctcta
    13261 cttccattgt tatttttcaa tatactgtat ttttatacag aacatattta ttaaacgatc
    13321 tttttcaagg ggaataaaaa ccaagacttg ccttatcgtt ctgcctagga tgaacaactc
    13381 aatactttca agaaaaatta gatagtgctg attcttcttt catcgctaaa aacacttctt
    13441 gcttcaatga tgggaggata attacgcaaa gaaaaaagga tatcagacaa ctgcccaata
    13501 tccttgacat agctaaataa gcatgcttct tcgtgtgcgc tcttccctaa aaaacagaaa
    13561 aataactagt aattctgtat gaaattaggt acaatataca cgtcgatgca caagcattga
    13621 ctatggcaac ttgattggtc agttcccgct gacttttcaa gccccgacaa tttagctgct
    13681 actaaattgt cgggtgcctt ttttagtttt tattcgtttt tcattctata gaaagcgctt
    13741 caataaatca agacattttt ttgttagaat gctaataata acaaaaggtt ttaactcttc
    13801 ttatgtacat tacaggtaaa aaaacaaaaa aatttctctt ttttttgaat tctgaacaaa
    13861 tagtactgga gtacaaaaga cgaggaagag ccactatctg ctcttcctcg tcttttgatt
    13921 ctcacctgtt attcatgatt ctttttctca gtttcccgat atttttccat tttacgacta
    13981 aaaagctctc cgctacttgg tggcgtatat gtgatggcat ttgctcctgc ttgaatggtc
    14041 tcagcaatac tttcttccgt tggaccacct gtcgcaatga taggaagttc aggatatttt
    14101 ttacgaaaat ggcgtactgc ttctgcagtt tctttacctg cactgatatt gataatcgaa
    14161 ataccagctg ctaacttttc atctatttct gtatatttcg acgttaccgt actgataact
    14221 ggaatatcaa ctactcgaca aacttctttt actgtctcta aaggcgtagg accgttcaaa
    14281 acgaccccta acgaaccttg cgcttctgcg aacatgctca tgtaggaaga acgagcacct
    14341 tgtgtcaaac caccacctac accggagaaa actggaatgt cagctgcctc aataatgctt
    14401 ttagtaatcg ctggatgagg tgtaaaagga tacacagcaa ttactgcatt cgcatttgta
    14461 ttacgtatga tcgcaatatc cgtagtaaag atgatcgaac ggatcaattt tccaaatatc
    14521 ttgatcccgc ttgcttctaa aatgatttcc ggtactctta cgatatcttt tctcaattcc
    14581 gtcatgattt ctggcgtaaa ttgttctgcc atcagccact tctcctttgc tatctccggt
    14641 catactgatt caattgatac gtctcgatga cctggatgat tttttgcgca taaccggggt
    14701 ctgtcgcata acctgcatct tgcaatgctt gtgcggcttc ctgatagttg gtggccgtaa
    14761 tcactttttc atacagcgca ggattccaat ctactccttg gacgaacagt tgtgtatggt
    14821 catccatgga ctgttcccat gaatcataca ctttgaagtc tccttggata gttatccatt
    14881 cttcgttaac aaattccttt gtttccaggc ttactttctt ctgatcccca taagccttga
    14941 tcccgaaaag attattgtat ttgctagcca aggtactttt tccccaattt gattctaata
    15001 tagcttgtcc taaaatgata ctaggaagca ctccataacc atcttgtagt tcttgggcat
    15061 gcggaacaag ccgttcaata aattgctgat gggattgcgc ttcattttct tgctgattat
    15121 cagtaaaagg agaagacaga ttcttcaagg aaaaaacaaa ggctaagagt atcactgcta
    15181 caccggcaac cactgctggc caacggatat tcttgcgctt ttgctttttc cacttttttt
    15241 tcgccatgat caattcctac ttttcattta attcattcag atattcttct agcgtcaatt
    15301 ggtgtttggt gatatactca gcgttttctt taccgacata acgtaagtgc caaggttcat
    15361 atgtgatctt agttatatct tcgtgtcctt ttggataacg aacgataaat ccatactgcg
    15421 cagcattatc tgcgatccat ttagctccag gctgatctcc gtaacttgca tccaatacag
    15481 tagaaggata actgttgtac cagttttgat cgacgacatc gactgccaga cctgtgtggt
    15541 gttcgctgta tcctggctcc gtaatcgttt ctttagtaat ctttgtcgcc tcctcttctg
    15601 atacaccttg acgactcatc acatcttgga cattttgttc gaaaacttgt tgttgggatg
    15661 atacagaccg gtaagctgag accatcacta atggaaatcc agctgcttca gcagccttgg
    15721 caaatgcttc atattctgga gcaatgcgtt tgtcgatcaa gtatccgtta cttaatgatg
    15781 ccaattgagt agactcatca atttcttttt ctaatttgtg atctggacca accaaaacta
    15841 gcttccaatc gtctgtttta acatctggca attgtttgga cgtacgatct tttgtttctt
    15901 tgtcctttac cttgctgctg gttgtttgac tgttcttttc tttactagca gttgttttgg
    15961 tgcttgttcg ttcgactgct tgattgccgg cttgaacatg cgaatcatca tcgctaaaaa
    16021 taacatagcc cattgctgcg atcataatca caactgctcc aaatcctaaa aatttattca
    16081 cttgaaaaat cctttcgttg ttcttcacgt tccccattcg cctcatgaac gtaagtttct
    16141 tccagatttt cattcaccca atcgagaagt tcgatttttt ctccttcgat ctgtttttga
    16201 acatctgctg gcagacgaaa attcaaaata tcatcatagc cccattcatc atattgaata
    16261 cttactttca agtcaaggta tctaccagcc tgctgattat ttttcaaagg gatcagctcg
    16321 acttttgctg ctttgctgct aagccatttt tgcgcgatgc gtgttttgat ttgcttgtac
    16381 tctgcaacgg caacgtttcg tttttcggga aaaataatcg tttgacgcaa aatcttttgc
    16441 gttagcatcc attcataatc agtgaaaaga ttttttactt tctgcatcgc ttcagatggc
    16501 agatttttct cgccatcttt ccatttttgc cattgttctg gactgatcag caagtattca
    16561 tctaaaaaat ttttttcagt ttcaaactgt tctaataata cactcataat aagatcaata
    16621 tatacttgat gcacgtttat cccctccttt taactattct acttaagttt tgatcaaaaa
    16681 gcacctctta aatcgctgct ttcatttttt ttaatgtttc aagtcctctc ctctttattc
    16741 ttcgtgattt ttgttataat aatagacaga caagaaactc aatccaacta tgaaggagcg
    16801 ttttcagatg aaaaaatatc aatggggaat cgttggtctt ggtaccatcg cacatgagtt
    16861 tgccgaaagc ttcaatcaag agacgagcga actaacagca gttgcttcca gaacttcaga
    16921 aaaagcggaa aacttcgctc accgttacaa tattccaaaa gcttacggaa gctatcaaga
    16981 aatgttagac gatgctgaaa tcgacatcgt gtacattgct gtaccaaata gacagcatat
    17041 cgaccatatc ttggcagcat tggaagcagg caaacacgtt ctatgtgaaa aagccatcac
    17101 gatgaataaa aaagaactgg ctgacgctat gcgcttagca gaagaaaaga atttgatctt
    17161 agcagaagcg atgacaatct tcaacatgcc actttatcag cagttacgct caataatgga
    17221 tacaggaaaa ctgggggctt taaaaatgat ccaagcacct ttcggcagtt acaaagaacc
    17281 agaccctaaa aaccggttct tcaatccaga attagctgga ggagctctgt tggatatcgg
    17341 tacgtatgct gtatctttcg ctcgtttctt tttgagctct caacctgaag tagttgcctc
    17401 tacaatggtt ccttttgaaa caggcgtgga cgaacaatcc gtcactatct tgcgtaacaa
    17461 agaaaatgaa cttgctgctg tcagcttgac ttttcaggca aaaatgccaa aagtcggtat
    17521 cgttgccttt gagaacggtt atgtgacgat tgctgattac ccgcgtgctg atcgtgcgga
    17581 gatcttattt acagatggta caaaggaatt catcgaaagc gggaatacat ctgaagcaat
    17641 gaattacgaa ataaagaata tggtacacat gattgaaggt gacttgccca atcgctctct
    17701 cttcttgaca aaagatgtca tcgaaatctt ggatcaaatg caagttctat ggaaaaatat
    17761 gtaattttaa
//
LOCUS       DBKOIJ010000048        17722 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000048 DBKOIJ010000000
VERSION     DBKOIJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.048"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..928
                     /locus_tag="ACV5NJ_001266"
     CDS             <1..928
                     /locus_tag="ACV5NJ_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HHY6485367.1"
                     /translation="FVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANN
                     RLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSK
                     TAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFF
                     SGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQ
                     RLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACD
                     QILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            897..2312
                     /locus_tag="ACV5NJ_001267"
     CDS             897..2312
                     /locus_tag="ACV5NJ_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HHY6485368.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            2345..3379
                     /gene="sppA"
                     /locus_tag="ACV5NJ_001268"
     CDS             2345..3379
                     /gene="sppA"
                     /locus_tag="ACV5NJ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HHY6485369.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            3381..3902
                     /locus_tag="ACV5NJ_001269"
     CDS             3381..3902
                     /locus_tag="ACV5NJ_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HHY6485370.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            3899..4285
                     /locus_tag="ACV5NJ_001270"
     CDS             3899..4285
                     /locus_tag="ACV5NJ_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HHY6485371.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(4781..4853)
                     /locus_tag="ACV5NJ_001271"
     tRNA            complement(4781..4853)
                     /locus_tag="ACV5NJ_001271"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4818..4820),aa:Lys,seq:ctt)
     gene            5234..5803
                     /locus_tag="ACV5NJ_001272"
     CDS             5234..5803
                     /locus_tag="ACV5NJ_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HHY6485372.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            5790..6650
                     /locus_tag="ACV5NJ_001273"
     CDS             5790..6650
                     /locus_tag="ACV5NJ_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HHY6485373.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            6840..7544
                     /gene="yycF"
                     /locus_tag="ACV5NJ_001274"
     CDS             6840..7544
                     /gene="yycF"
                     /locus_tag="ACV5NJ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HHY6485374.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            7549..9384
                     /gene="walK"
                     /locus_tag="ACV5NJ_001275"
                     /gene_synonym="yycG"
     CDS             7549..9384
                     /gene="walK"
                     /locus_tag="ACV5NJ_001275"
                     /gene_synonym="yycG"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HHY6485375.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            9381..10697
                     /locus_tag="ACV5NJ_001276"
     CDS             9381..10697
                     /locus_tag="ACV5NJ_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YycH family regulatory protein"
                     /protein_id="HHY6485376.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            10698..11567
                     /locus_tag="ACV5NJ_001277"
     CDS             10698..11567
                     /locus_tag="ACV5NJ_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HHY6485377.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            11622..12431
                     /locus_tag="ACV5NJ_001278"
     CDS             11622..12431
                     /locus_tag="ACV5NJ_001278"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HHY6485378.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(12534..13163)
                     /locus_tag="ACV5NJ_001279"
     CDS             complement(12534..13163)
                     /locus_tag="ACV5NJ_001279"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HHY6485379.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            13333..14625
                     /locus_tag="ACV5NJ_001280"
     CDS             13333..14625
                     /locus_tag="ACV5NJ_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHY6485380.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(14701..15363)
                     /locus_tag="ACV5NJ_001281"
     CDS             complement(14701..15363)
                     /locus_tag="ACV5NJ_001281"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HHY6485381.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            15588..15872
                     /gene="groES"
                     /locus_tag="ACV5NJ_001282"
     CDS             15588..15872
                     /gene="groES"
                     /locus_tag="ACV5NJ_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HHY6485382.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            15923..17548
                     /gene="groL"
                     /locus_tag="ACV5NJ_001283"
     CDS             15923..17548
                     /gene="groL"
                     /locus_tag="ACV5NJ_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HHY6485383.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6056 a         2893 c         3695 g         5078 t
ORIGIN      
        1 ttttgttatg gataatagaa ttagtctagg tcaacttatc acatacaatg ctttactagt
       61 atttttgaca gatcctttac aaaacattat taatttacaa gtgaaaatgc aaacggctca
      121 agttgcaaat aatcgtttaa atgaaatttt cgcgattgat tcagaaaaat cgaaccagtc
      181 ttttgaatat atgctttctg aacaattatt tcaacaagat atcactgtaa aaaacgtttc
      241 attctcgtat agcatgaagg ccccaacgtt gaacgaaatt tcatgtacaa ttccttcgca
      301 cagtaaaacg gctatagttg gagttagtgg ttctggaaaa tcaactcttg caaagttatt
      361 tgttcgtttt tatgaagcat cagaaggatc gatccattat ggaaacatta atcacttaga
      421 cattcctttt cagatattac gcgatcgagt gacatatttg cctcaagagt catttttctt
      481 tagtggaaca atcattgaga atcttatatt cggtttagat agcccaccaa gtttcgaaca
      541 aataatagaa gtttgtgaga tagtccaact aaatgaattt attaatcaac agccattacg
      601 ttacgagaca ttgttggaag aaggtggaaa taatctttca ggagggcaac gacaacgatt
      661 agccttagca cgggctttat taaaaaatcc ggacatatta attttagatg aagcaactag
      721 tggattagat accttgttag aacatgcaat tatgaattat ctcctcaatt taaaagaaaa
      781 aacgattatt tttattgcgc atcatttacc aatagcaaaa gcatgtgatc aaattttagt
      841 tctccatgaa gggcatttgg tagaaaaagg aacgcacgat gaacttcgtt ttgccgatgg
      901 tatctatcaa aaattatggg aaatctagga gagggaaaca atttatgcag aataataatt
      961 ggttcgaaca ttctagtatc tatactaaac aacatcatag cttctaccgt tgggtgttat
     1021 accctgtaat tcttttttta tgtattattg gattgttttt aacctttgct aaaaaagagg
     1081 ttgttattcg gacaacggct caattgaacc ccgaaaaaat agagaagtta caagtgccat
     1141 tagaagctaa aataatggaa aacttcctaa aagaaaatca atttgttcat aaggacgatg
     1201 tgttggttag attggattgt tctttaatag agaatgaaaa ggctcagata gaacaagaaa
     1261 accaacgtat tactcaacaa ataaagatgg ctcagctatt tattgaaagt ataagtaaag
     1321 gaaaaaattt gttttcaacg gatgacagtt ttggctacag taatcaatta aagagcatgt
     1381 tgtcagaaaa agaatcactc cgctacgctt tgaagcaaag tgaattaaat gatcaaaagc
     1441 aattagaagt atacgaaaag acaaaaagac aactagaaaa acaaattgag agttcagata
     1501 gtaaattaca agaatggcaa caagtacagg tagcttggag taataatcaa tcattaaaag
     1561 atttttcaaa agaaatgatg gcaaactatg agaattggca agaacaacta aataatgttt
     1621 ctgatgatca aaaaaatcaa gtgaaactga caatttcagc aagcataaat gaacaaattg
     1681 agcaactaaa aaaagaagta gaacagtatc agtcagaaaa agctaaatta gttaaaccaa
     1741 ctacttctga gaatgacaga attagtcaaa cggaaaaagg aaagcaagag ctagaacaag
     1801 caataacaac tgtaaaacaa acagttacag agctaaatga aaaacaagag aagaaccagt
     1861 cagttatcaa aagtttggac gatcaacttt caaaaggaat actaaaagct cctgtatctg
     1921 gaacggttca tttaaatgaa gaaacaaagg gacaagcaga actagccaaa ggaacagtgc
     1981 ttgcagaaat ttatccgatc cacgaaaaat caaaaatgaa atttacagca cttttacctg
     2041 cgaatgaaag tacctatatt aaagaaggga tgaaggttca ttttaaattg gatcaaaaag
     2101 gtggggctcc gattacgatc gacggaactt tagatgaaat ttcagaaaat tctactgcca
     2161 ctgagaaagg tgttttttat gcagtgaaag gaacgttaaa aacaactcaa tcgtttccgt
     2221 ttcgttatgg attaactgga gagttgtctt taattgtagg agaaaaaaca tattggcaac
     2281 aaattgtcga tgtgtttcta aataaaaaat aattattttt actaagaata taggaggagt
     2341 ttatatgaat aaaagaagat ggattgctgt gggagtagcg tgcggattat tgttactatc
     2401 cattatcgtc gcgctaattc caggaaaaga taaagaggaa gcatcaaata caaccctaac
     2461 aggaataaat aagatttttt acggatctaa cgaaatcaca gaagaaacgt tagaagaagg
     2521 cgcatcaaat aaaaagattg tcaaactatc cgtcaatgga gttattgcag atacaggtga
     2581 aagcaatctt tttagtaggg aacaatataa ccatcagaat tttttaacac agctaaagaa
     2641 aattcaagaa gacaaagcgg ttaagggagt gttattagaa gtaaattcac ctgggggagg
     2701 aatatatgaa agtgctgaga ttgctaaaga aatggcaaaa attaaaaagc tagatattcc
     2761 tatctatact gcatttaaaa atacagcggc aagtggaggg tactacattt ctgctggttc
     2821 agataaaata tttgctacgg aagaaacaac aactggatca attggtgtca ttatttctgg
     2881 tttaaattat tctggcttat tagaaaaact tggcgtaact gatgcgacgt ataaaagtgg
     2941 tgcattaaaa gatatgatgt cacctcaaca caaaccttca gaggaagaga ataaagttat
     3001 acaggaattt gtgatgagtg cttatgatcg ttttgtcaat gttgtagcaa aaggaagaaa
     3061 tatggatact aatgcagtga aagaacttgc ggatggaaga atttatgatg gtaaccaagc
     3121 agtagaaaat gggttggtgg atcaaattgg ttactctgaa gatgcattag attccctaaa
     3181 aaaagagaag aaattaactg atgcgacggt tatagaatat aaaaatgaca caactggctt
     3241 tgcttcttct tggttaggta ataagattgc tgaatggcaa gggctaaagg caactacatc
     3301 agatcgtgta atttcagttt ttgaaaaact tggattttct gatactccaa aaccaatgta
     3361 ttactatgga gggctataaa atgaataaga agagtggcat tgaaactttg cctaattatg
     3421 tatttgcggg attttggtta cgattttttg cttttcttat tgatttaatt tgtattagca
     3481 tcatcaccca tgctactata ggtctctttt acaaaatcat gggactggaa tctgacaaat
     3541 ccattattac tttttatggt atagcgtcat tagctattta tttaacctat tttgtacttt
     3601 tgactaagct gaattcagga caaacaattg ggaaaatgat ttttgggata agagtagtga
     3661 gctttgagga aaaagagcta agttggcaaa ctatctttat tcgtgagtgt atatgtaggt
     3721 ttatattaaa aaattttgta tttcaaattg ggtatttggt ggctgctttc acgcccaaga
     3781 aacaacatat tggggattat ttttctaaca cgtcagtggt tgttattcgt tcattagaag
     3841 ctattaacta tggtcaaaaa atttctaatt ctaaaacact taatctggag agaattaaat
     3901 gaataagaga aaaacagagt ttattttgat tttaattagt ggaataacag ggattttatg
     3961 tagtctttta gcattgatgg gtggtatgtt tctatcttct attgattcaa ctactttaaa
     4021 tactacagaa ctaaccagta aggatataga tatgttagtc aatctaagcg ggaatgttgt
     4081 tttatttgga ggaattaccc tagttttttc gataatgatt ttcataatag gttttttagt
     4141 aagaaaaaat caaaaaacag tggtctatgg tattagttta ctcattttat ctattattcc
     4201 attttttctt ttaacattat tgtgggtaat tcctggagtt ttaggtatta ttgcgggatt
     4261 gatgcttcta ataagaaaat cttaattagg caatgtgtag acaacgtgaa acgattaatc
     4321 aattaaaagc taaatacgtt ttgagaaatg ataacttgta gtcgatggaa gacatgtaga
     4381 taaaaaaact acgtgtcttt tatttattct aattttttcg aaaaaaagac actctctgat
     4441 tgttgtatta atggaaaagg ggaaagcagc aagtaaattt agtttttcta ttaaatattc
     4501 ttcacttttt aaccttttat agtacttaga tttaatccta tatgtttaga gagttcattg
     4561 gcagcatgat tttgcaaagc ttctcgaatt attgttatat tagaaacttt actgttcata
     4621 attatgcttc ttttttaact caaagtccaa agggctacag tggactttga gttaaaaata
     4681 aaaagcccta gttattttga aaaataaaaa aagaacagcg taaatgtttt cgaaatgctt
     4741 tcgcaccaac atttacctgt tccgtgggtt tggcacccaa tgggccgtga ggggctcgaa
     4801 cccgcgaccc gctgattaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa
     4861 aagtacttaa taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat
     4921 cagaaaaatc tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta
     4981 ttatataagc ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc
     5041 attttttact cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac
     5101 aagaaggctt agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag
     5161 ttaaagcaac tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa
     5221 aggagcggtt atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga
     5281 aacattccgt ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt
     5341 cagaacgtta gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct
     5401 tacctcttct atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa
     5461 aagatataac aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt
     5521 gactattcct gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga
     5581 agcctataat ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc
     5641 acaaggtaaa tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt
     5701 aagagcggca actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga
     5761 aaccgaatat ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag
     5821 attcaggtac ggatgttcca gaagaattga taaaaaaata cgagatgtat atgatcccat
     5881 tgaaaattat ttataaagat caggtatata cagataaaat tgacattacc cctgaagaga
     5941 tctatcaacg tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca
     6001 cagaaatctt tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt
     6061 ccagtggatt gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat
     6121 tggaagcata tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc
     6181 aagcagcaaa atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg
     6241 agttagtcga aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag
     6301 gtggacggat cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca
     6361 tctcatgtaa tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa
     6421 gtttggataa aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact
     6481 tagctgttgc ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa
     6541 gagagttccc acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc
     6601 acactggtcc gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa
     6661 ggaaacactg aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa
     6721 taaagtattt tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca
     6781 aaagcagaaa tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta
     6841 tgaaaaaagt tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt
     6901 tagccaaaga aggctatgac gtttatactg catatgatgg ggaagaagcc ttggaaaaag
     6961 ttgctgaggt agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct
     7021 tagaagttgc tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca
     7081 aagattctga aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca
     7141 agccattttc caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca
     7201 cggctgcgaa ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga
     7261 tccatccaga agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg
     7321 aattcgaact actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt
     7381 tattacaaac ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg
     7441 tacgtcgttt gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac
     7501 gtcgtggtgt cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa
     7561 gtccgctttt ttcgatcagt caattttaag atcgctatta cctttattct gattttattg
     7621 atctcgatcg aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat
     7681 acatttatta aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa
     7741 ttgaatcgaa aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt
     7801 gaaaacagtg cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc
     7861 cgaggaacaa ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat
     7921 atcaatgatt tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat
     7981 atcaatgttc aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc
     8041 aaaagtaata tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact
     8101 gcctcactaa ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt
     8161 acacagccga tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt
     8221 cggaaagtca aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag
     8281 ttagctgaac gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat
     8341 agcgtgctat cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt
     8401 acgatcaatg atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa
     8461 tcgattttga cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat
     8521 ccaaacgaaa tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg
     8581 cgagtagact tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc
     8641 ttgcatgatg tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat
     8701 gtttcccatg aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc
     8761 gaaggtgcgt ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa
     8821 acagaccgaa tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt
     8881 aatacccagc ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac
     8941 cggtttgata tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg
     9001 caaagagatc tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc
     9061 atgaataacg cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa
     9121 acgcataata acgtgatttt aagtatcacc gatcaaggat tggggattcc taaaaaagat
     9181 ctgaaccgag tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt
     9241 gggacaggac ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt
     9301 tgggcagaga gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct
     9361 tatgaggagg aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt
     9421 tgatggttgc gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa
     9481 gttcaatcaa cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa
     9541 cttccgaagt ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa
     9601 ccaacagtga aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa
     9661 agctttctga agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact
     9721 ctggttttga gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg
     9781 atttagattt agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa
     9841 tacaattcga tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat
     9901 atgaagcaaa tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca
     9961 caaaatggac agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac
    10021 attcgatcaa actgaagaag tatagttata tcctgtcaca gcaaccctat acacttttta
    10081 gaaatgcttt tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga
    10141 ttttttacgg tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc
    10201 gtggagagct gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata
    10261 ttagcaaatt aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga
    10321 tcgattatcg gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga
    10381 ttggtgtgga agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt
    10441 tgaatacgat tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg
    10501 aaatcatcca gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg
    10561 ttggctatac atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat
    10621 ggtatgtgaa atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg
    10681 gattggaggc gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt
    10741 tagggttgaa tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg
    10801 tcattcgctc taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt
    10861 acacggggaa gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca
    10921 atcttagcac ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg
    10981 gtgtgttttt agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga
    11041 acgtgatcga tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca
    11101 atattttatt cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac
    11161 cactagaaat ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa
    11221 aaatcgttct ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga
    11281 cgcatttatc tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag
    11341 caatcaatac attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc
    11401 agctagctta ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc
    11461 atgtagcgat cgaaacaagc gataaagtgg tccaaattga gagcgtcaac gctttcagca
    11521 atacgatcgt gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata
    11581 atgaaaaaag tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag
    11641 cttttaaaat cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg
    11701 aaaaaaaacg gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg
    11761 ctcaaattga tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag
    11821 atcatataca tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc
    11881 agcagacttg ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc
    11941 tatttgagat ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct
    12001 cccatgacgc agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca
    12061 tgttgacgga caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg
    12121 gttatttgat ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga
    12181 atttaaaaca gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg
    12241 tcatgactga tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg
    12301 aaaacaatat gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt
    12361 ttggcgttgg acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat
    12421 tttcgattta aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg
    12481 gttggtttta tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga
    12541 ctgactacac tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca
    12601 atcgcgtaat ctgccatgct gatcgtactg tcgctagcag cgtttctttg taaataatca
    12661 tcacttaaca aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg
    12721 atataagtcc aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg
    12781 aaagctgttg gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt
    12841 tctgtatcta agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct
    12901 tcaagcatct caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga
    12961 gcgttgaaag catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat
    13021 aaatcctttt gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc
    13081 gtcacgtcta aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta
    13141 gctccaatga gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca
    13201 gttgctgaat attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat
    13261 ggttcgtgat cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga
    13321 aggagataag aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt
    13381 gtgaatctta gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt
    13441 attgttggct cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg
    13501 atagacaaaa caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg
    13561 atagcctttt ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat
    13621 cgcttaccag tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat
    13681 actcagatag atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg
    13741 ctcatcatcg gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga
    13801 gagaagatac ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata
    13861 gtcactttta cgcttatcgg ttcagcggtt tttcaacatt tgttcagaat gactgttgtt
    13921 caaggagtat ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt
    13981 gtgcttttat taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg
    14041 atggtcaatg ggttggttag tcccgtcttg caagcgaatt cattagcaaa tgcagaaatc
    14101 ttagcctcag gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt
    14161 ctcgatcagt tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg
    14221 acggtttttg cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc
    14281 tttttcaaca tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg
    14341 gctgttcctt ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat
    14401 tttggattcg ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc
    14461 tcgggtttct tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt
    14521 agttttttca tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa
    14581 agacagacaa aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc
    14641 ctgtcgcttt agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt
    14701 ttatactagc cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac
    14761 gtgtgtgatc atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa
    14821 acctaggaaa aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga
    14881 gctgatcact gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg
    14941 gcggaatacg aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg
    15001 tgcttccatg atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa
    15061 caatacagat tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac
    15121 caatccataa ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa
    15181 gggttcacgt tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac
    15241 tgcagttgct gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa
    15301 gggtgagata aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga
    15361 catgtaattc ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt
    15421 tcaattatat cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta
    15481 aaagcttgca aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg
    15541 ttaagagtgc taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat
    15601 taggtgatcg cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg
    15661 ttttagcttc tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag
    15721 gccgtttgtt agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta
    15781 tgttcgaaaa atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg
    15841 caggaaaaga tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa
    15901 taatcaatga ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc
    15961 agctatgtta cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa
    16021 aggccgaaat gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt
    16081 gacgatcgca aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt
    16141 atcagaagta gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt
    16201 tctgacacaa gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct
    16261 aggcattcgt cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat
    16321 ctcaactgtt gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc
    16381 tgataaagtt ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat
    16441 cacaatcgaa gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt
    16501 tgatcgtggt tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct
    16561 agaaaaccca tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc
    16621 attgttagaa caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga
    16681 tggtgaagca ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc
    16741 tgtaaaagct cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt
    16801 gacaggtggt acagtaatta ctgacgatct aggtttagaa ttgaaagatg caacaatcga
    16861 aaacttagga aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg
    16921 ttctggagaa aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga
    16981 aacaacttct gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg
    17041 tgtagctgtt gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg
    17101 cattgaagat gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg
    17161 tggtacagct ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt
    17221 ggcaacaggt atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga
    17281 aaacgctggt tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac
    17341 tggtttcaat gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc
    17401 aactaaagta acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac
    17461 gacagaagca gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga
    17521 tccatcaatg atgggcggta tgatgtaaga aaaagcaact tgcttatcta caactgatca
    17581 ttagtgaaag acagataaaa agaggctgga acagaagcgt ttaactccaa gaaataagaa
    17641 gaaattcacg gaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt
    17701 gcttctgctt ccgctgttta ta
//
LOCUS       DBKOIJ010000049        17304 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000049 DBKOIJ010000000
VERSION     DBKOIJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.049"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(31..576)
                     /locus_tag="ACV5NJ_001284"
     CDS             complement(31..576)
                     /locus_tag="ACV5NJ_001284"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HHY6485384.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(588..1280)
                     /locus_tag="ACV5NJ_001285"
     CDS             complement(588..1280)
                     /locus_tag="ACV5NJ_001285"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HHY6485385.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1299..1583)
                     /gene="macP"
                     /locus_tag="ACV5NJ_001286"
     CDS             complement(1299..1583)
                     /gene="macP"
                     /locus_tag="ACV5NJ_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HHY6485386.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1596..2159)
                     /locus_tag="ACV5NJ_001287"
     CDS             complement(1596..2159)
                     /locus_tag="ACV5NJ_001287"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HHY6485387.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2387..3049
                     /locus_tag="ACV5NJ_001288"
     CDS             2387..3049
                     /locus_tag="ACV5NJ_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HHY6485388.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3173..4369
                     /locus_tag="ACV5NJ_001289"
     CDS             3173..4369
                     /locus_tag="ACV5NJ_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HHY6485389.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4470..5846)
                     /locus_tag="ACV5NJ_001290"
     CDS             complement(4470..5846)
                     /locus_tag="ACV5NJ_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHY6485390.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5883..6749)
                     /locus_tag="ACV5NJ_001291"
     CDS             complement(5883..6749)
                     /locus_tag="ACV5NJ_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HHY6485391.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6838..7863)
                     /gene="sapS"
                     /locus_tag="ACV5NJ_001292"
     CDS             complement(6838..7863)
                     /gene="sapS"
                     /locus_tag="ACV5NJ_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SapS"
                     /protein_id="HHY6485392.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7881..8555)
                     /gene="sapR"
                     /locus_tag="ACV5NJ_001293"
     CDS             complement(7881..8555)
                     /gene="sapR"
                     /locus_tag="ACV5NJ_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SapR"
                     /protein_id="HHY6485393.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8739..9632)
                     /locus_tag="ACV5NJ_001294"
     CDS             complement(8739..9632)
                     /locus_tag="ACV5NJ_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HHY6485394.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9706..11403)
                     /locus_tag="ACV5NJ_001295"
     CDS             complement(9706..11403)
                     /locus_tag="ACV5NJ_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HHY6485395.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11396..12709)
                     /locus_tag="ACV5NJ_001296"
     CDS             complement(11396..12709)
                     /locus_tag="ACV5NJ_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HHY6485396.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12709..16650)
                     /locus_tag="ACV5NJ_001297"
     CDS             complement(12709..16650)
                     /locus_tag="ACV5NJ_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HHY6485397.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16759..17217)
                     /locus_tag="ACV5NJ_001298"
     CDS             complement(16759..17217)
                     /locus_tag="ACV5NJ_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHY6485398.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4847 a         3893 c         3010 g         5554 t
ORIGIN      
        1 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
       61 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg aatctttgaa
      121 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
      181 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
      241 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
      301 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
      361 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
      421 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
      481 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
      541 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
      601 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
      661 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
      721 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
      781 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
      841 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
      901 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
      961 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
     1021 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
     1081 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
     1141 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
     1201 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
     1261 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
     1321 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
     1381 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
     1441 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
     1501 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
     1561 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
     1621 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
     1681 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
     1741 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
     1801 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
     1861 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
     1921 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
     1981 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
     2041 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
     2101 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
     2161 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
     2221 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
     2281 tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa
     2341 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
     2401 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
     2461 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
     2521 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
     2581 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
     2641 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
     2701 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
     2761 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
     2821 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
     2881 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
     2941 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
     3001 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
     3061 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
     3121 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
     3181 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
     3241 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
     3301 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
     3361 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
     3421 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
     3481 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
     3541 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
     3601 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
     3661 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
     3721 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
     3781 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
     3841 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
     3901 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
     3961 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
     4021 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
     4081 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
     4141 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
     4201 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
     4261 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
     4321 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
     4381 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
     4441 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
     4501 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
     4561 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
     4621 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
     4681 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
     4741 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
     4801 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
     4861 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
     4921 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
     4981 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
     5041 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
     5101 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
     5161 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
     5221 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
     5281 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
     5341 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
     5401 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
     5461 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
     5521 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
     5581 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
     5641 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
     5701 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
     5761 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
     5821 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
     5881 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
     5941 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
     6001 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
     6061 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
     6121 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
     6181 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
     6241 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
     6301 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
     6361 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
     6421 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
     6481 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
     6541 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
     6601 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
     6661 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
     6721 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
     6781 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
     6841 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
     6901 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
     6961 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
     7021 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
     7081 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
     7141 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
     7201 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
     7261 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
     7321 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
     7381 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
     7441 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
     7501 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
     7561 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
     7621 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
     7681 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
     7741 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
     7801 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
     7861 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
     7921 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
     7981 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
     8041 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
     8101 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
     8161 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
     8221 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
     8281 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
     8341 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
     8401 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
     8461 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
     8521 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
     8581 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
     8641 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
     8701 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
     8761 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
     8821 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
     8881 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
     8941 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
     9001 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
     9061 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
     9121 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
     9181 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
     9241 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
     9301 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
     9361 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
     9421 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
     9481 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
     9541 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
     9601 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
     9661 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
     9721 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
     9781 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
     9841 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
     9901 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
     9961 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    10021 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    10081 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    10141 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    10201 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    10261 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    10321 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    10381 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    10441 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    10501 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    10561 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    10621 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    10681 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    10741 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    10801 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    10861 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    10921 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    10981 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    11041 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    11101 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    11161 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    11221 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    11281 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    11341 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    11401 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    11461 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    11521 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    11581 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    11641 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    11701 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    11761 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    11821 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    11881 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    11941 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    12001 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    12061 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    12121 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    12181 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    12241 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    12301 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    12361 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    12421 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    12481 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    12541 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    12601 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    12661 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    12721 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    12781 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    12841 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    12901 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    12961 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    13021 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    13081 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    13141 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    13201 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    13261 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    13321 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    13381 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    13441 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    13501 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
    13561 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    13621 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    13681 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    13741 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    13801 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    13861 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    13921 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    13981 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    14041 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    14101 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    14161 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    14221 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    14281 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    14341 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    14401 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    14461 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    14521 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    14581 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    14641 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    14701 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    14761 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    14821 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    14881 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    14941 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    15001 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    15061 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    15121 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    15181 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    15241 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    15301 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    15361 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    15421 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    15481 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    15541 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    15601 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    15661 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    15721 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    15781 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    15841 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    15901 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    15961 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    16021 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    16081 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    16141 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    16201 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    16261 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    16321 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    16381 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    16441 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc
    16501 gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag ctgttggccc
    16561 aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg cttgaccgct
    16621 gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt gaatttcagc
    16681 ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc tttcatcaac
    16741 caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg tttcaaaaat
    16801 caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc
    16861 gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg
    16921 aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc
    16981 ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc
    17041 aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt
    17101 ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg
    17161 tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc
    17221 cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa caatgcccat
    17281 ctaattataa tttaatttaa tttt
//
LOCUS       DBKOIJ010000050        17185 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000050 DBKOIJ010000000
VERSION     DBKOIJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.050"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            47..1387
                     /locus_tag="ACV5NJ_001299"
     CDS             47..1387
                     /locus_tag="ACV5NJ_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHY6485399.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1605..2048
                     /locus_tag="ACV5NJ_001300"
     CDS             1605..2048
                     /locus_tag="ACV5NJ_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HHY6485400.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACV5NJ_001301"
     CDS             complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACV5NJ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HHY6485401.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4373..5596)
                     /locus_tag="ACV5NJ_001302"
     CDS             complement(4373..5596)
                     /locus_tag="ACV5NJ_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HHY6485402.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5627..6466)
                     /locus_tag="ACV5NJ_001303"
     CDS             complement(5627..6466)
                     /locus_tag="ACV5NJ_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HHY6485403.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6483..7163)
                     /locus_tag="ACV5NJ_001304"
     CDS             complement(6483..7163)
                     /locus_tag="ACV5NJ_001304"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HHY6485404.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7165..8238)
                     /locus_tag="ACV5NJ_001305"
     CDS             complement(7165..8238)
                     /locus_tag="ACV5NJ_001305"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HHY6485405.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8294..8532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8575..8808)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8885..9556)
                     /locus_tag="ACV5NJ_001306"
     CDS             complement(8885..9556)
                     /locus_tag="ACV5NJ_001306"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6485406.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9643..12291)
                     /gene="mgtA"
                     /locus_tag="ACV5NJ_001307"
     CDS             complement(9643..12291)
                     /gene="mgtA"
                     /locus_tag="ACV5NJ_001307"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HHY6485407.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12633..12807)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12980..13417
                     /gene="ndk"
                     /locus_tag="ACV5NJ_001308"
     CDS             12980..13417
                     /gene="ndk"
                     /locus_tag="ACV5NJ_001308"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HHY6485408.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13439..14305)
                     /locus_tag="ACV5NJ_001309"
     CDS             complement(13439..14305)
                     /locus_tag="ACV5NJ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HHY6485409.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14465..16558)
                     /locus_tag="ACV5NJ_001310"
     CDS             complement(14465..16558)
                     /locus_tag="ACV5NJ_001310"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HHY6485410.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4966 a         3565 c         3037 g         5617 t
ORIGIN      
        1 tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa
       61 attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg
      121 tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat
      181 tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt
      241 tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc
      301 tcattctgct gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc
      361 tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt
      421 atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt
      481 cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat
      541 ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca
      601 gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt
      661 gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc
      721 agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc
      781 cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg
      841 cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga
      901 tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc
      961 atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc
     1021 tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta
     1081 tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc
     1141 cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc
     1201 actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg
     1261 attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct
     1321 gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa
     1381 tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga
     1441 aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc
     1501 tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca
     1561 tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga
     1621 agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg
     1681 tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg
     1741 gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga
     1801 taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac
     1861 gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta
     1921 tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg
     1981 aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa
     2041 tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca
     2101 agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc
     2161 tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag
     2221 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg
     2281 ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt
     2341 tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc
     2401 gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat
     2461 ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac
     2521 aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta
     2581 ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca
     2641 tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa
     2701 taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta
     2761 ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat
     2821 aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa
     2881 tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct
     2941 ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta
     3001 atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc
     3061 taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc
     3121 cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag
     3181 cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta
     3241 aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca
     3301 cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa
     3361 agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca
     3421 aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat
     3481 ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg
     3541 ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta
     3601 cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga
     3661 tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa
     3721 tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga
     3781 tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca
     3841 ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg
     3901 caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt
     3961 tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata
     4021 tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt
     4081 tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc
     4141 actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat
     4201 cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt
     4261 tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt
     4321 tcatttttct gaaaattatt tttataactt tcacacacaa gattattttt tactagaaag
     4381 tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta
     4441 atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg
     4501 caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg
     4561 caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat
     4621 ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa
     4681 cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat
     4741 catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac
     4801 ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac
     4861 gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg
     4921 taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat
     4981 taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat
     5041 gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt
     5101 tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt
     5161 ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat
     5221 atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata
     5281 cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga
     5341 attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg
     5401 ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa
     5461 tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta
     5521 gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg
     5581 agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga
     5641 atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt
     5701 cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc
     5761 ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg
     5821 catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt
     5881 ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt
     5941 ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga
     6001 tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga
     6061 ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc
     6121 ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt
     6181 aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag
     6241 ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt
     6301 tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta
     6361 cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa
     6421 taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac
     6481 tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag
     6541 attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc
     6601 gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg
     6661 atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa
     6721 tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc
     6781 ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca
     6841 aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta
     6901 gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc
     6961 ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact
     7021 aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata
     7081 tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa
     7141 tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc
     7201 taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc
     7261 ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc
     7321 ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag
     7381 atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt
     7441 attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag
     7501 atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg
     7561 gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat
     7621 ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt
     7681 caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc
     7741 ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg
     7801 tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag
     7861 ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa
     7921 agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat
     7981 cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt
     8041 ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa
     8101 cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt
     8161 taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac
     8221 atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg
     8281 aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt
     8341 tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc
     8401 atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc
     8461 ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt
     8521 caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag
     8581 gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc
     8641 aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg
     8701 acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga
     8761 gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt
     8821 gggattaatc tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct
     8881 atatttattt ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt
     8941 ccgcaagaag tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat
     9001 caggtatcaa aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat
     9061 cttccactgc aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag
     9121 gaatcggtga aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat
     9181 cagctaattt aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa
     9241 tgcctagttt catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta
     9301 agtggatggc tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt
     9361 cctgtacttt ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt
     9421 tttttccgcc ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc
     9481 cccaaagcca gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa
     9541 atattgcacc tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg
     9601 actggtccaa aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa
     9661 tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc
     9721 aaataaccat aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact
     9781 tgtaaaagga aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa
     9841 tgttgcgttg ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt
     9901 ttgtgtccat agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg
     9961 ctgtgaacta tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata
    10021 catcaacaag taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa
    10081 ttccccaata ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg
    10141 atcccatggc aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa
    10201 aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc
    10261 aaaattagaa cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc
    10321 agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga
    10381 ttctttcgca atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc
    10441 aggagcgtca ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc
    10501 tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc
    10561 tacttgtgct aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt
    10621 gatctctaga cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag
    10681 aacttttaca gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt
    10741 tggtggatca aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga
    10801 aaattctcca acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc
    10861 ttcattaagt gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt
    10921 aacagtaccg ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcaccttt
    10981 tgtgatcatt tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc
    11041 aaaatcaaaa ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc
    11101 tgttgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct
    11161 atttagatac gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata
    11221 ttcaagaatg attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat
    11281 tgcaccaaag ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac
    11341 acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc
    11401 aactgctaca gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt
    11461 gatgacgata actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt
    11521 atggatgcct aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg
    11581 accaaatagt gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac
    11641 attacttccc ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca
    11701 tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa
    11761 taaatccttt gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg
    11821 aacaagatct ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt
    11881 tacagctgcc gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac
    11941 ggactgaatc aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc
    12001 gaaaagatct ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc
    12061 taatgcgatc aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa
    12121 tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat
    12181 ttgttcttct gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt
    12241 ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc
    12301 tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat
    12361 cgatagagaa aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc
    12421 tagatccttg tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat
    12481 tttaattcat gacttccaat catttttcga atagttattt ttccaattag accacctcct
    12541 ttttattttt caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc
    12601 gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca
    12661 ctgtacaacg taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga
    12721 caattcctgt tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta
    12781 taggagcctc acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa
    12841 gaggatgtca aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat
    12901 tcctgtctca cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag
    12961 taggaaacga ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag
    13021 acgccattta gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga
    13081 aatgaagttt gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga
    13141 gcgcagtttt tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt
    13201 gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc
    13261 cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat
    13321 tcacgcttct gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt
    13381 cgatataaac aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct
    13441 agttgctggg ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc
    13501 ctaacatata ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta
    13561 aagcagcaga aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac
    13621 ttctttcttt atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca
    13681 accgaccatt ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct
    13741 catgattgtt caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct
    13801 caatgaaccg atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac
    13861 catagtctgg tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt
    13921 caaattctgt ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat
    13981 acagatcttc tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa
    14041 agctgtcttc ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat
    14101 ccagctggtt tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg
    14161 atctcaatgt tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag
    14221 tcttaaaaca gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt
    14281 tcttgatcaa ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat
    14341 tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt
    14401 cataaaaaat accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga
    14461 ttgtttattg attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg
    14521 ttgttgtttt attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt
    14581 cgattgcttc attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct
    14641 caaatccgtt tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac
    14701 ttaacgcagt cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt
    14761 tcgtatcgat gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt
    14821 tgggtgtcac aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata
    14881 agtctgtacc aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg
    14941 gcaaaacatc gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca
    15001 tatggatcat caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg
    15061 gatctttacc aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat
    15121 ccccgtaaat cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa
    15181 attgttccaa tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat
    15241 tgccagtatt caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt
    15301 tggttacgga aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag
    15361 attctctgaa taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa
    15421 aataagatcg atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc
    15481 aaaaactagc tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga
    15541 tcgctggtgc ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag
    15601 gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta
    15661 cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa
    15721 acggtgtgac ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta
    15781 aatggagaac gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg
    15841 cataatgttt ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc
    15901 gaacactgct agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta
    15961 gatatttgac aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt
    16021 cgctcagcgc tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca
    16081 tcggtttgcc gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta
    16141 gcccaatgaa cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa
    16201 aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa
    16261 tgaaatctgt gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa
    16321 tatcaatgat caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc
    16381 caaaaaacaa taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac
    16441 cttccacacc taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga
    16501 aaacagctgt caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt
    16561 atatcacttc tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct
    16621 aaaaaagttg ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc
    16681 acagcataaa accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg
    16741 tgaccgcttc actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag
    16801 gctgtgacaa aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa
    16861 cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa
    16921 aacatgagaa gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca
    16981 agacctcttg atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg
    17041 ctaattttac aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    17101 aaccaaacga aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa
    17161 acggcaggct caagacctct tgata
//
LOCUS       DBKOIJ010000051        16968 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000051 DBKOIJ010000000
VERSION     DBKOIJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.051"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1152
                     /locus_tag="ACV5NJ_001311"
     CDS             <1..1152
                     /locus_tag="ACV5NJ_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HHY6485411.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACV5NJ_001312"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACV5NJ_001312"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HHY6485412.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACV5NJ_001313"
     CDS             2728..3003
                     /locus_tag="ACV5NJ_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HHY6485413.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACV5NJ_001314"
     CDS             3009..3776
                     /locus_tag="ACV5NJ_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HHY6485414.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACV5NJ_001315"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACV5NJ_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HHY6485415.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACV5NJ_001316"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACV5NJ_001316"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HHY6485416.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACV5NJ_001317"
     CDS             7920..8222
                     /locus_tag="ACV5NJ_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HHY6485417.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACV5NJ_001318"
     CDS             8225..9388
                     /locus_tag="ACV5NJ_001318"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HHY6485418.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACV5NJ_001319"
     CDS             9414..12842
                     /locus_tag="ACV5NJ_001319"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HHY6485419.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACV5NJ_001320"
     CDS             12863..13987
                     /locus_tag="ACV5NJ_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HHY6485420.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACV5NJ_001321"
     CDS             13968..14414
                     /locus_tag="ACV5NJ_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HHY6485421.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACV5NJ_001322"
     CDS             14398..14736
                     /locus_tag="ACV5NJ_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HHY6485422.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACV5NJ_001323"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACV5NJ_001323"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HHY6485423.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACV5NJ_001324"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACV5NJ_001324"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HHY6485424.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACV5NJ_001325"
     CDS             15893..16936
                     /locus_tag="ACV5NJ_001325"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HHY6485425.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5749 a         2935 c         3746 g         4538 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacga
//
LOCUS       DBKOIJ010000052        16373 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000052 DBKOIJ010000000
VERSION     DBKOIJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.052"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(1..279)
                     /locus_tag="ACV5NJ_001326"
     CDS             complement(1..279)
                     /locus_tag="ACV5NJ_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485426.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            472..1128
                     /locus_tag="ACV5NJ_001327"
     CDS             472..1128
                     /locus_tag="ACV5NJ_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HHY6485427.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(1570..2367)
                     /locus_tag="ACV5NJ_001328"
     CDS             complement(1570..2367)
                     /locus_tag="ACV5NJ_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HHY6485428.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(2364..3548)
                     /locus_tag="ACV5NJ_001329"
     CDS             complement(2364..3548)
                     /locus_tag="ACV5NJ_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HHY6485429.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3573..4820)
                     /locus_tag="ACV5NJ_001330"
     CDS             complement(3573..4820)
                     /locus_tag="ACV5NJ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HHY6485430.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            5035..6780
                     /gene="dnaX"
                     /locus_tag="ACV5NJ_001331"
     CDS             5035..6780
                     /gene="dnaX"
                     /locus_tag="ACV5NJ_001331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HHY6485431.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6800..7114
                     /locus_tag="ACV5NJ_001332"
     CDS             6800..7114
                     /locus_tag="ACV5NJ_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HHY6485432.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            7139..7735
                     /gene="recR"
                     /locus_tag="ACV5NJ_001333"
     CDS             7139..7735
                     /gene="recR"
                     /locus_tag="ACV5NJ_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HHY6485433.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7799..8443
                     /gene="tmk"
                     /locus_tag="ACV5NJ_001334"
     CDS             7799..8443
                     /gene="tmk"
                     /locus_tag="ACV5NJ_001334"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HHY6485434.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8458..8787
                     /locus_tag="ACV5NJ_001335"
     CDS             8458..8787
                     /locus_tag="ACV5NJ_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HHY6485435.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8801..9739
                     /gene="holB"
                     /locus_tag="ACV5NJ_001336"
     CDS             8801..9739
                     /gene="holB"
                     /locus_tag="ACV5NJ_001336"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HHY6485436.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9775..10599
                     /locus_tag="ACV5NJ_001337"
     CDS             9775..10599
                     /locus_tag="ACV5NJ_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HHY6485437.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10592..10939
                     /locus_tag="ACV5NJ_001338"
     CDS             10592..10939
                     /locus_tag="ACV5NJ_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HHY6485438.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            11008..11880
                     /gene="rsmI"
                     /locus_tag="ACV5NJ_001339"
     CDS             11008..11880
                     /gene="rsmI"
                     /locus_tag="ACV5NJ_001339"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HHY6485439.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11989..13110
                     /gene="dinB"
                     /locus_tag="ACV5NJ_001340"
     CDS             11989..13110
                     /gene="dinB"
                     /locus_tag="ACV5NJ_001340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HHY6485440.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(13164..13766)
                     /gene="nrdG"
                     /locus_tag="ACV5NJ_001341"
     CDS             complement(13164..13766)
                     /gene="nrdG"
                     /locus_tag="ACV5NJ_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HHY6485441.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(14081..16270)
                     /gene="nrdD"
                     /locus_tag="ACV5NJ_001342"
     CDS             complement(14081..16270)
                     /gene="nrdD"
                     /locus_tag="ACV5NJ_001342"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HHY6485442.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         5110 a         3039 c         3403 g         4821 t
ORIGIN      
        1 ttaagctgaa tatgctttta atttttgtat ttgaacttct acatcgtgat aattggatgg
       61 atagcgatca acaagttggc gaataaataa atctcttgta gacataaaca gatttaaaaa
      121 ttcttcgcat tcgtttgttg gattctcatc cactgctttg atcaattcga tcgagttttg
      181 atacgcatca tttaaaaaga ctaaagaagc tttcatatca aatcttgttt gatatgctgt
      241 ttctaatcga ttaatgattg tgttcatcca gtaattcatg ttttttccct ctattcttaa
      301 aaaattttac ttctgcagta taacgaataa aaaaaaggaa aaaaatcgca taagtgatgg
      361 aaaaaggtac ttaacaaatg ttaatatcat tagtaaaata cctttggact ttaaaaagtt
      421 ttaaagtact gtatgatctt tttagatgaa agtttggatg ggggagcatc attggatagt
      481 agagtcttaa aggaatattt ggataaaaat gattttccga ttatacgcaa aggatatcat
      541 aaatatttga cattcgaagg gatggaagat tcccatacgt atatcttgaa agagggaacg
      601 gtaaaagcaa gtgttatttc aaatgatggg agagaattta accttcgcta tatcaaagat
      661 cttgaaatcg tctctctttt aaaagacgaa tattcacaat tcatcgattc gccgtataat
      721 attcgagttg aatcagaata tgcagagttt tatcgaatca atcgtgtgcg cttttggcaa
      781 aatatcaacc aagacccaaa gctacaacac tatgtaaaag agtattaccg ctatcgtctg
      841 ctttattcga tgaaaaaaat gcagcaaatg ctattaaacg gaaagctggg agctgtttgt
      901 acgcaacttt acgaattata tgatttattc ggtgtgcgga cagaagaagg gatgctgatc
      961 gatttttcga tcacgaatga agaaatcgcc aaattttgtg gcatctcaac tgctagcagt
     1021 gtaaacagaa ttttaaaaca actgaaaaaa gatggtatta tcaccgttaa agaccatcat
     1081 atcgttattt tgaatatgga cttgttagaa gacaatatta tcttttgatg aacaaagagg
     1141 ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
     1201 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttaccaagag
     1261 gttgtgagcc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa cagcttttca
     1321 tgtttgatga atattgtgac ttgtgaccga ggagttggta ggagaacacc gtttaccaag
     1381 aggttgtgaa aaagctgttc tgcatcaagt aataagaaga gccaagtaaa aacagtctct
     1441 tatgttttta acttggctgg acttatcacc gtagatgtaa gcttttgaac gccgtttatt
     1501 tgcaacaaaa ggggctgtga cacgattttt gtcacagccc tttttttgcg aacattcatt
     1561 tagcaatttt tagaaacttc tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt
     1621 agtacttccg ccaccgccgc ttccaggagg tgggcttttg gggattcttc ttgtcgtgac
     1681 aaaagaattt gtcagacgat cggttttttc agttaatttg atactggatt tttctcggaa
     1741 tggatatttg tagatgcctg atttcaattg ataacgcgaa acagtcacaa aataaaagac
     1801 aagactaagg ataagagcca aaacaacagc aatgatgatt tctactgtcg tcaaaacctt
     1861 atagcgtgta attttgccag tttcttcgtc tacacgataa tgtcctcctg gaactccgtc
     1921 ttccacatat gttgctgatt tagacaggta agcttttgca gcattgtaat aggcagcatt
     1981 tgtcatctga tcataaacat catctaagat cgaatcgatt ctgctatcag tcaaataatc
     2041 gatcatatta ccagaagtcg aaatgtagat ttcacgctgt cccatatcta atagcaatac
     2101 tgagccattt tgatcatttc cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg
     2161 ttcttctgaa ttagatgtag tcgttacgat caagactcgt cctttgattt tttcattcag
     2221 cttttctgcc tgttgttcca aagattggat ttgatctgtt gtgaataacc cagcctcatc
     2281 atttaccgta gctgtatctg catgagtagc caccggccag ccaacactta ctagcaaaag
     2341 aaagaataaa aaaaattttt tcatcatatc aagtaacctc cgatcagaaa gaggatcgct
     2401 agcccagcga aaatcccaag agcggtcagt cccaattttt tctggctgat cggcaggaca
     2461 ccgctgacct ttcctgtttg tccattcatt gcgtagtagt agactttttt acttgagtca
     2521 ttgcttcgat aagtaacaag ccataccggc agtaatacgt aattatttac ttcgtttgtg
     2581 attgaagcag aagttcgcac accggtcaat gttgtatatc catttgcggt atcttttaac
     2641 agcttctctg aatagtcttt taattcagat tgaatctgtg atttgattgc ctcatattcg
     2701 atatctcttt tttctgcttg gaacccggct aaatactggc ttttaaaatc aattgcttta
     2761 tctaacggga aaggttgcac gccttcgacc atcttttgtt gcgtattttt tgataaggca
     2821 ttttttacta gttctttaaa agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt
     2881 tctgtgtatt cgatgtcacc tatccgccat atgcggataa ctgtaccatt cgcctgcatg
     2941 gcaccatcta cttcagcatc tgtagcccaa taagggaaat acacacctgt caacttatcg
     3001 atttgatcct tattgaaaaa agctttggga acaaaccatt ttttcttggt ccaagcgaga
     3061 aatctgttga ttgcttcttc tttctcgaca gcaaaaggaa gtactttatt tggcaagaat
     3121 tctccgctta aacgtcccga tagtacgacg ggattatggc agtagtaaca ataagtcgca
     3181 gcagttgtcg catcagtcac gatctctgca ccacagttgg gacaaagaaa aagatctatc
     3241 gctgcggtat tatctgcaga ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc
     3301 gctttgcgct cggcttcatt catatcggca agctgttctt gggctgtaaa agtaaaatct
     3361 tcggtaggtg aaggttcttc tttgctttct tcgccagcca tacgcgcttc tttttgcttt
     3421 tgctcatatt ggctgacttc ttcttctgta tagatattca ggcagtattc gcaatgaaat
     3481 ttttggtctt tgggatcaaa agtcaacgga ccgccgcaat taggacattt atgtgtaaga
     3541 acatccatca gctgcacacc tttctaaata aattaattaa cggggattcc tttaaacggt
     3601 ttaccacagt ttggacagaa tttagggatg ccgttggaaa gatccacgat ctcattgcat
     3661 tcactgcatc gcatttgtag cttaggctga acgttttcta caggtttggc agctccgcag
     3721 ttggaacaga attttccgga attttctgtc ccgcaaactg gacatgtcca agtatccgca
     3781 tttccttgtg cagtttgttg attttgtttt tctgcttgtt gtttgatttg ttcttggtta
     3841 ttttgagctg cttgagaaag ataagaacca tttgcattca ttcccatacc tacacccata
     3901 aagccggtca ttgctcctgc ttcatttttt ccggctgctt ccataccacg tgcaatggaa
     3961 ccttgcacgt agccttcacg tacgctagga tcacctagca tcgccccttc gttacgcata
     4021 ttgatcagtt tcacagaatc atcggtgtaa gaaatgctgg ctacagccac agaaacaatc
     4081 tccatcccac gtaattcacg ccaagaatca tccaatgcag tatccatgta tttgcttaat
     4141 tccaaactct tagatggtac ataagaaatc cgttgaccat ctgcagacat ctgattgatt
     4201 gctgattgta gcgctgttag gaattcagct aaatactgct cattgatatc attgatttcc
     4261 acttgtgttt tgtttttagg gattgcgttt gtataaaata aaatcgggtc aacgacatga
     4321 atagaataag tgccatgtgc ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag
     4381 ttcaacggcg cgcttgtacc gaatttgatt ccctttattt cttggagatt gatataaaaa
     4441 acttgttgtt tctgcggtgt gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt
     4501 gcttctttca gtgttccatt gaacatggaa ggagctgcgt catttttaat ggtgtaataa
     4561 ccttcctcag cggtataatc gatgattttt ccaccatcga ccaaaagcat catcatgttt
     4621 ggatagacat ggacgactgt tccgtctgtt aaaacatctt ctgttccttt acgattggag
     4681 ccacgtttgt catctttgcg gactttgaca ccttttgtca tgaccgttgt atcactcata
     4741 ttgtctggtt cgatgacttc tagccattga tctgccagac cgccgccaac catacttgtt
     4801 gctgctttga taagacccat aaaaaattcc tcctctgatt cagctagtgc caaacaatta
     4861 ttgtttattt attataccat aggaagatgt gaaacttctc ggactagaga cggaattgaa
     4921 atggaaaatt gcgtattgtt taaatacctg aaaaagccga gaaggatagt agttttttta
     4981 cctaatatca cgtataataa gagatagtga attttttaaa aaagggggat ccttatggca
     5041 tatcaagcac tttatcgagt ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa
     5101 gctgtgacac agacactaaa aaatgctatt gtttcccaca aaacctcaca tgcctacttg
     5161 tttacaggac ccagaggaac cggaaagacg agtgcagcaa agattttcgc aaaagcaatc
     5221 aactgtccga acagtaaaga cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt
     5281 actgctggta cgcaagaaga tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa
     5341 gaaatccgat ttatccgtga tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg
     5401 tacattattg atgaggtaca tatgctttca acaggagctt tcaatgcctt gttgaaaaca
     5461 ttggaagagc caaaagaaag tgtcatattc atcttagcaa cgaccgaacc tcacaaaata
     5521 ccagctacga tcatttcacg gactcagcga tttgatttta aacggatcaa tacggcagat
     5581 attgtcgaac atcttgaatt tatcttgaaa gaatcgacta tcagctatga accagctgca
     5641 ttgaatgtga ttgcccgttc tgcagaaggc gggatgcggg atgcattgag tattcttgat
     5701 caggcaatct cgtttagtga aggaacaatt actttagatg atgccatgca agtgacaggt
     5761 agcttaactg acgaaatgat ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca
     5821 gaagcattgg aaacattgaa ccaaattctc gcagccggca aagaatcacg tcgctttcta
     5881 gaggatatgt tacaatactg ccgtgacttg ctgatgtatc agcaagcacc taacctagtt
     5941 gaggaacaat cgagtacgat cagaagtaca tttacagaat tggcacaaac tgtttcagcg
     6001 gatcagctgt tttcaatgat aaagattttg agcgatactc aaaatgaaat tcgttttacc
     6061 aatagtgccg ctatttattt ggaagtcgct acagtcaaac ttgcacagct accgtctgct
     6121 tctgttcctt cagcagatgt agcatttgca gatgatcaga tagtaaaaca gctacaacaa
     6181 gagattaagg aactaagaaa cgaaatcaag gaactacgca gtaatacagg aattgagaat
     6241 acaaaagtac agccggtaca gcaaacaaat cataaaaaac agacaagtac ctatcgtgta
     6301 ccgacggaac gagtatataa agttctgcgg gaagcgacaa gacagcatct gaaccaagta
     6361 aaagaagtat gggaagactt gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa
     6421 gcaagcgaac cagttgcagc tggacctaca ggactagtga ttgcgtttga ttatgagatt
     6481 gtttgtcaaa gagcagcaaa tgacgaagaa ttgcagcttt cggtacacaa caacttaagc
     6541 cgaatgatca aagattatgc gcctgatcct gtttatatta caagagaaag ctggccagaa
     6601 cttcgaagaa attatttgac gcaggcacaa gaagagggaa cagaaaactc ttttgcttct
     6661 gaagactcag aaggagaaat cgagttgctt cctcaagaac aagaagtacc agaagaaaca
     6721 cttgtagtaa aagcagagga actgtttggc tccgaagctg ttacagtggt agatgattaa
     6781 taaaaaggag tagataatta tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca
     6841 agtacaaaaa atgcaaaaag aaatggcaga agcacaagag ctgctaaata aaacagagtt
     6901 tacaggtaaa gccaccaatg aattggtcaa agcggttttt acaggcgatc gtcgcatgaa
     6961 agatatccaa atcaatgaag aagtcgttga tcctgatgat atcgagatgc ttcaggatct
     7021 tgtcgtaatg gcagtcaatg atgcgatcgc acaaattgat gtggaaactg agaaaaaatt
     7081 agggaaatac gcaaaaggtt tgccaggatt ttagttttaa actgaaggag taatgaatat
     7141 gcaatatcca gaaccaattg cgaaactgat cgacagctac atgaaacttc caggaatcgg
     7201 tcagaaaaca gcgacaagac tcgcctttta tacgatcgat atgaaagatg aagtagtaaa
     7261 tgaatttgct aaatctttgt taagtgtaaa acgcgatttg catttttgta gtatctgcgg
     7321 aaacatcaca gaagaagatc cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt
     7381 tatcctagtg gtcgaagagc cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca
     7441 cggactttat catgttctcc atggtgtgtt atcaccgatg gaagggacag gtccagaaga
     7501 catcaatatt tcttctctgt tgcagcggct gcatgatgca gatgtaaaag aagtgattat
     7561 cgcaacaaat gcaacaacag agggagaagc aacagctatg tatctctcac gtctgatcaa
     7621 acccgcaggt atcaaagtga ctcgtctagc acatggtctg tctgtaggct ctgatatcga
     7681 gtatgcagat gaagttactt tattgaaagc tgtcgaagga agacgtgagt tataaatttc
     7741 taattaagtg ttttttcaga tacaatataa aagcagcaca agagatgggg gcaacaatgt
     7801 gaacggacta tttattacaa tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa
     7861 tgaactttac cctcttttga aagaagtagc aaaaacagaa atcattcaaa caagagaacc
     7921 aggaggcatt ccgattgctg aagagatccg agcagtgatc cttgatccaa aaaatgatcg
     7981 gatggatgaa cgtacggaag cactgcttta tgcagcagca agaagacaac atctagtcga
     8041 aaaggttctg cctgctctgg caaaagggaa aatcgtttta tgcgatagat ttgtcgacag
     8101 ttctcttgct tatcaaggtg caggccgacg catcggcgtt accgagattg ctcggttgaa
     8161 tgagtttgct acagaaggta ctacgccgga ttttaccctt tatcttgacg tagattcaga
     8221 caccggatta agaagaatca agaaaaacag acaaaatcaa atcgaccgtt tagattcgga
     8281 aggtttggaa ttccaccagc gtgtacgtca tgcttactta aagctggcag aagagaatcc
     8341 ggaaagaatc cacaaagttg atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta
     8401 tcatacaatt attgaacagt atccgcaatt ttttgaaaac taggaaggtg aacgtttatg
     8461 aaaattattt tagcgattat ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt
     8521 gatgcaaata tccgtgccac aaaactatca tcgacaggcg gcttccttaa ggctggaaac
     8581 agtacgttta tcgtgggtat tgaagatgac cgtgtagaag aagcattgga aatcatcaaa
     8641 aaaacgtgtg aatcaagaaa gcaatttgta tctacacctg ttacactgga tatttcgatg
     8701 gatgggggcg tcccttatcc agtagaagta gaagtaggcg gtgccacagt cttcgtctta
     8761 cctgttgaag gattccatca attttaggag gagctgagct atgaatcaag aaataaactt
     8821 ggctcaaatg caaccgatcg tgtatcagca gcttcaacgg agttttgagc atgggcgtat
     8881 cgctcatgct tatctttttg aaggggagaa aggaacagga aaacatgaag taggtatatg
     8941 gcttgcacag cacctgttct gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa
     9001 caattgtcag cggatcgaaa agaaagaaca tccagatgtt ttggttattg aaccggaagg
     9061 acaaaccatc aaagtcgatc agatcagacg tttgcagacg gaattcagtc gcagcggtta
     9121 cgaatcaaga aaaaaagttt ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc
     9181 aaacagtttg ttgaaattct tggaagagcc ccctggagat tttttagcgg tgttagaaac
     9241 agatgcagta ggaagaattc tccctactat tcaatctcgt tgtcaagttc tgcattttca
     9301 agaactttcg aaagaagcac tgatccatcg attgcaagaa gagcagattc cattggaaaa
     9361 agcaaaactt ttggctttct taacaaacag tttaggaaaa gcagttgaaa tctctcaaga
     9421 tgaatggttt aatgatgcta aggacttgat ttatcaatgg ttcgtttact tgcaaaagca
     9481 agatactcaa gcctttatct atgtccaaaa aaaattggta aaatcattta aagaaaaaac
     9541 acaacagttt acaggattat ctgtcttgat gttttattat caggaagcac agcgtcaagc
     9601 agtagcaaac ggcgaatatg gcaaagtgaa acagataaac cagataattg aacggatatt
     9661 gatcgcagag cagaaactac gcagcaatgt cagttttcag gctgttgcgg aacagttcgt
     9721 tttgcatacg attttttagc ttgccgcaaa ggatgatgat caaggaggat ggaaatggta
     9781 gaagtagtag gtgtccgata tagagatgca ggtcatatct attattttgc accaggagac
     9841 tctgaatatg tttatagtga caaggtatta gtcgaatctc agcaatcctt acatattgct
     9901 actgttgtcc ttcctaaaaa agagattgat cccaatgacc ttcctgaagc tatccgtccg
     9961 atcttgcata aagcgtcaca agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct
    10021 tatgatgcat tgaaaatcgc aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg
    10081 gtcaaagccg aatacacatt tgatcgaaat aaattgattt tttctttcac agcggacggg
    10141 cgaattgact ttagagaatt ggtaaaagat ctggccgctg tatttcgtac tcgtattgag
    10201 cttcgacaaa ttggtgtccg cgatgaagca aagataatag gcggaattgg tccatgtggg
    10261 cgtccattat gctgttcttc ttttttaggc gatttcgtgc ctgtatcaat caaaatggca
    10321 aaagatcaaa gtttatcttt gaatccaaca aaaatttccg gtttatgcgg acgcttgatg
    10381 tgctgtctgc aatatgagag cgaagaatac gagacagcga aaaaagaact ccctgacttt
    10441 ggaaaagtaa ttgctacacc tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc
    10501 cgtatcgtta gtattcggct gactggacgg gaaaatcctg tcgaatatga atgggatgaa
    10561 ttgaaagaat ttatccaagt aggtgagatg aatggataag agatcattat acgacggatt
    10621 gaatcaatta gaatcagagt tgagaaatac attaacagaa cttgttgaaa tgaagcatgc
    10681 tttgcatgag atcgtagaaa aaaatacaac tttggagatg gaaaatcaac gtctgcggga
    10741 acacctgcgg gaattgaacc aagttgcaca agctccagct gaaaatgtga agcaggaact
    10801 ttctaagtct cgtatgaatc ttgaaaaaat ttatgaagaa ggattccatg tctgctacga
    10861 tttatatgga tcaagaaggg acaatgacga gccttgtgcg ttttgtctag aagtgatcta
    10921 tcgcgaaagc ggtcgatgac caataacaag aggagactgg agcggagaaa tactgctcca
    10981 gtctttttat taaggagatt gtgacagatg tataatcaaa aaagtttcaa gggaaatccg
    11041 gtatatgggg atttatatct tgtaccgacg ccaattggaa atttagaaga tatgagctac
    11101 agaagtgttc gtatgctgca agaatccgat cttatcgcaa gtgaggatac gcgtaataca
    11161 caaaagttat tgaatcattt tgaaatccat acaccccaaa aaagtttaca tgaacataat
    11221 tataaagaac gtgtccctca attgattgct ttgttaaaag aaggaaaaac gattgctcaa
    11281 gtcagtgacg caggaatgcc ttccatcagc gatcctggac atgagctagt tttagcctgc
    11341 ataaaagaag ggatttctgt gatctctatc ccaggaccta cagctgggat gacggctttg
    11401 attgcttcag ggctagtgcc tcaaccattt ttattttatg gcttccttcc acgtaagaaa
    11461 aaagaacaga aagaaacatt gaaaaagcta aaagaacaga cagttacgct gattttttat
    11521 gagtcacctt atcggatcac tgctacattg acgaatttct tagaaatttt tggaaatcgc
    11581 caagtcgttt tgtgtcggga attgacaaag gtccatgaag aataccttag aggaagtacg
    11641 gaagagctac tcgcttatat cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta
    11701 gaagggaatt tgttcgaaat gccagtgacg gaagttgatg aagagttggg aacgttaaag
    11761 gaacaagtag aagcaaagat cgcttcaggc gaaaaaccaa atgctgctat caaagcagta
    11821 gcgctgaaaa atgggctgaa aaaacaagaa gtctaccgtc agtaccatga attagactga
    11881 gatagtataa gttgtctata atgaaagact aatgaggaaa tcagctacta acttttcaaa
    11941 tctcttgaac gcgaacgaat gttcttgtat aatgaataag aggtgaggat gatggagctg
    12001 caatttcctt tacataatga tacatcaaga aaaatcattc atattgatat ggatgctttt
    12061 ttcgcttcgg ttgaagaacg agatcatcct gagtttcgcg gacatccatt agtaattgcc
    12121 agacacccca gtgacactgg tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga
    12181 aaattcggaa ttcattcagc aatgagtgca caaaaagcgt atgagctttg tccgcaagcc
    12241 atctttaagc ctggcgacca tcataaatac agcgatattt ccaaacaagt acgggaagta
    12301 ttctatcgtt atacagatat tgtggaacca gttagtatcg atgaagcgta tcttgatgtg
    12361 accgaaaata aaatcaattg caagtcagcc atcaaaatcg cccgaatgat ccaagcagat
    12421 atttgggaga gtgtccagct aacatgttct gcgggagtaa gttacaataa gtttttggcc
    12481 aaattagcat cagactatca aaaacctaga ggaatcacgg tagttgcccc tcaagacgct
    12541 gtgccatttt taaaagcttt gccaattgaa aaatttcatg gggtagggaa gaaaactgta
    12601 cctagaatgc atgagctagg gatttataca ggaaaagacc tatatgaatg cacagaaatg
    12661 atgttgattc gaaattttgg aaaaatgggc tattctcttt atcgtaaagt cagaggaatc
    12721 catgattctc cagtaaacgt tacaagagag cgaaaatctg tgggaaaaga gcatacttat
    12781 gggcagccgt tgcaaacgga agaagcagtg ctgactcagt tgcgccaact agctgaaaaa
    12841 gtagaagaag cactgagaag agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg
    12901 tacacggact attctactgt gaccaaaaga gtgacattgc cagaatatat ctataaaaaa
    12961 gaagcactct tttatcaagc aagccttata tgggaggaaa tcttgggagt agaaaaagga
    13021 atccgattgt tagggattac tttgacgaat ctggacccga tgacttatga aaacatcgtc
    13081 cttccattat gggaaaatca agaaatataa aaacagcttt gagagttgaa accggaaatc
    13141 cgttttctct cccaaagctg tttttatatc aagtcttgct ttttgatttg ttcataggtt
    13201 tctgttgcat ccgtacattt ttcccaaatc acaacttgat tggattctaa agatttttgg
    13261 acgtcaatga tccgctggtt gctacttccg cgaaattgta gttttaggtc acgtttgctt
    13321 aattcaaagc gtccgtcaac aaggatatcg atttgcgaaa ggagttctaa tttatcttct
    13381 gaatcttgta gcaattcatc aaaggtatag cctgtccatg accagatatc ttttgtcgta
    13441 ccgaactctt ttcggatacg tcgtacgact tgcagacaaa catctgtatt caaaaaaggt
    13501 tcgccaccaa gtaaagttaa tccttgcacg taatcgttgc gcaagtcagc gatgatttgt
    13561 tcttctaatt caggtgtgaa tggtttgcca tatcgaaaat tttgtgcagc tttgttgaag
    13621 cagccttcgc aggcaaataa gcagccacta acgtaaagac tgttgcggac accttcacca
    13681 tcgacaaaat taaacggttt gtagtcagca atatagtttt ggctgtagtc ttttgctaac
    13741 cattcttttg gttttggatt tctcataaaa aaacttcttt catttaaggt tcaaagatat
    13801 agctattgaa gataaaatct actcgaccac ataaataaga acaaactgac ctaaatccat
    13861 tgttcaagaa agcttgttct tattttgata gagggcgttc aaagatacaa agccatcgaa
    13921 agagggtggt gaaaaaagac ttgtcatagc ctcttattct gaataaacgg tgttcaaaag
    13981 cttgcatctg cagtaataag cccaactaaa cgaaaaatat gaggaactat ttttgtttag
    14041 ctattcttat tacttgatgc aggacagctt tttcacaacc tcatttcatg tgtttcacac
    14101 gtgaggaaat ttctttatgg cggccatgta ccatcggacg agcttgcgga tttcctaaat
    14161 agccacaagt acgtttgact acatcacatg tttttggatc atgatttcca cattctggac
    14221 attcgaaccc tcgttctgtc ggtttgaaat caccttcgaa accacattga tagcaatgat
    14281 cgattggcgt attcgtacca agatacccga ttttgtcata tgcaaaatcc catacagctt
    14341 ctaaagcttt cggattttgt tggagcatag ggtattcaca gtagtggatg aatccacctg
    14401 aacaatattt tggataatcc ttctcaaatt ccaatttttc aaatggcgtt gggtttttac
    14461 gcacatcata gtggaaactg tttgtataat attctttatc tgtgatattt tcgatgatgc
    14521 cgaatttttc tgtatccaaa cggcagaaac ggtcagttag actttcactt ggagttgaat
    14581 agacactgaa atgataacca tattcatccc cccatgcatc tgcattagct tttaattctt
    14641 tgacgatatc aagtgtgaaa gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga
    14701 aagctgaagc aacttcatac aatccaatgt agcctaacga tacagtagct cgacggtttt
    14761 tgaataattc attgacagaa tcggtacgtg aaagacgttt cccgaaagca ccatacatat
    14821 ataaaattgg cgcatttgct ggaatggctt ctttacaacg ttctacacga tagagaagcg
    14881 catctttcat gatttctaag cggtcagaca gtaactgcca gaatttattc tgatctcctt
    14941 ttgattcgat cgcgattctt ggcaaattca atgtaacaac acctaaattc attcgtccga
    15001 catttacttc ttgaccattt tcgtctttcc acccttgcaa gaatgaacga cagcccatag
    15061 gtactttgaa acttccagtt aattcaacga ttttatcgta attcagaata tcaggataca
    15121 ttcttttcgt tgcacattcc aatgctagtt gtttgatgtc gtaattagga tcttcagggt
    15181 ttaagttcac accttttttc aaagtgaaga tcagtttagg gaagatagct gtccgctttt
    15241 cactccctaa tccattgatt cgaatctgta aaattgcttt ttgaatttct ctttcaaacc
    15301 agttttctcc tagtccaaat cctaatgaag tgaaaggagt ttgtccattt gaagtaaata
    15361 aggtattgat ttcatattcc aaactttgca tagcatcgaa gatatctttt ttggtttttg
    15421 cttttgcata ttctttttgg cgttctggcg aatcgatcca ttcttccgcg tcttttagat
    15481 gttttttata gttcaattct gcaaaaggag caagcaattc atctgttcgg tcggcagaac
    15541 agccaccata ttgacttgac gctacattgg caataatctg tgagatttgt gctgtggcgg
    15601 tctggataga ttttggcgat tcgacttcgg cattgccgat tttgaaccca ttgttcagca
    15661 tgcctttgaa atcaatcaag cagcaattcg tcatcggtgt ataaggatga taatccaaat
    15721 catggtaatg gatatcgcct ttttgatgcg catttgctat atgtggtggc aacattttca
    15781 gtccgattga ttttccaacg atcccggctg tcagatctcg ctgtgtattg aaaacattac
    15841 tgtctttatt tgcgttttcg ttcacgacag tttggtcctt gttgattagc ttttcaatcg
    15901 aaaagttgat atctgttgct ttactgcgag caaaatctcg tttggtacga taattgatgt
    15961 actcttctgc taattcatat tcgttttttt ctagtaatat atgttccacg atgttttgaa
    16021 tctcatagat tttgacattt tcagcaaacc ggcttttgat ttcttggaca accaagtctg
    16081 caatcatttg aattttttca taagtaagtg gtgataatga gccgtgaatt ttcttttctg
    16141 ctttgatcag tgcttcattg atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt
    16201 tgatgacttt catctgggat gtctctggct gctgagtaac gacagttttt tcttcataat
    16261 ttactttcat tatcttcaac tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg
    16321 ggaaggagca actaaatatc ggctatatat agtattcaat gtgctgtata att
//
LOCUS       DBKOIJ010000053        16294 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000053 DBKOIJ010000000
VERSION     DBKOIJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.054"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(35..898)
                     /locus_tag="ACV5NJ_001343"
     CDS             complement(35..898)
                     /locus_tag="ACV5NJ_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HHY6485443.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1094..3193)
                     /locus_tag="ACV5NJ_001344"
     CDS             complement(1094..3193)
                     /locus_tag="ACV5NJ_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HHY6485444.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3413..4522)
                     /gene="rpoD"
                     /locus_tag="ACV5NJ_001345"
     CDS             complement(3413..4522)
                     /gene="rpoD"
                     /locus_tag="ACV5NJ_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HHY6485445.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4546..6387)
                     /gene="dnaG"
                     /locus_tag="ACV5NJ_001346"
     CDS             complement(4546..6387)
                     /gene="dnaG"
                     /locus_tag="ACV5NJ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HHY6485446.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6569..8884)
                     /locus_tag="ACV5NJ_001347"
     CDS             complement(6569..8884)
                     /locus_tag="ACV5NJ_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HHY6485447.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9069..9929)
                     /locus_tag="ACV5NJ_001348"
     CDS             complement(9069..9929)
                     /locus_tag="ACV5NJ_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HHY6485448.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(9988..10620)
                     /locus_tag="ACV5NJ_001349"
                     /pseudo
     CDS             complement(9988..10620)
                     /locus_tag="ACV5NJ_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            10787..11548
                     /locus_tag="ACV5NJ_001350"
     CDS             10787..11548
                     /locus_tag="ACV5NJ_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHY6485449.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11574..14165
                     /gene="mprF"
                     /locus_tag="ACV5NJ_001351"
     CDS             11574..14165
                     /gene="mprF"
                     /locus_tag="ACV5NJ_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HHY6485450.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14353..15540)
                     /gene="tuf"
                     /locus_tag="ACV5NJ_001352"
     CDS             complement(14353..15540)
                     /gene="tuf"
                     /locus_tag="ACV5NJ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HHY6485451.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15908..16264
                     /locus_tag="ACV5NJ_001353"
     CDS             15908..16264
                     /locus_tag="ACV5NJ_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HHY6485452.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4457 a         3378 c         2875 g         5584 t
ORIGIN      
        1 tataagctag ttaagaaaaa catgcaaggg attctcaatt agattttttg actagttttg
       61 tcacaccgtt ttcttgtgta atcaatcctt gtttcaaaag attacctaat gcgcgcttaa
      121 atgctccttt gctgatacca aaagctttca tgatttcttc tggatccgat ttatctgtaa
      181 agttgatttg ctgatctgca gaacgttcta aaatggttaa aatcatttgt gcatcatctg
      241 atatagcttc atatgctctt ggcttcaatg aaagattcag tacaccgtct ggtcggacac
      301 caatcactcg accttttatc tgctcgccta gacgaggttc attatatcgt tcagaaggat
      361 ggataaaccc aagatagaaa tcttccgtta aaacgtacgt acctgttaat ttcaagcggt
      421 atgctgttcc tatcacatct ttgtttttca tttcttctgt tccatatttg cttaaagatt
      481 tgaatatttt ttcatctgct aaactgcccc aaatacggtc ttttgcatca actctaaggg
      541 aaatcatcaa gcgatcccct tttttaggcc atagctctgt cattgtcggc aattcatcaa
      601 gagatacgac aaagtcttta tctggaagac cgatatcgac aaacgcacct aaatcttttc
      661 gtgtagcagt aacgattcca aaagcgtaat gaccgatacg gcttttagga atttgtgtag
      721 tgatccgatt ttcatgtttt tgattttggt aaccgaatcc ttcaactgct tcaccgattt
      781 tgtgttcacc ttctgatttg cttaagccaa aggtttgtcc gtttttttgg acaaagtagt
      841 ttttttcatt ttcatcaata ataatacctg taaatatagt tgctaataaa tcgttcattt
      901 ttttctccct ctatagtgaa aggttttctt aatacatttc tttgctatca taacataatg
      961 ttatctactt gaatagctga gccttggttt ggaaaggtga aaaaaggagt atgacaacag
     1021 ttgtgtcata ctcccctttt tcgaataaac ggtgttaaac ggcaggctca cagcctcttt
     1081 catttgccta aaattaattg ctcccatttc ctccgaacca tccagtttta cagttgatgg
     1141 ttacgatgta aaatggcaaa gcactttctc caatgccttt catcaatgca gattttgttg
     1201 aagtggcata tagattataa tagccttccc cattttcgat tctggctttt tctaatctgt
     1261 aacctccttc aacgatatat ccacgttgga tacatgtagc aatcactttg ctttggacac
     1321 ctggtgccca cgtccatgct ggattagatg catcggcaat atcacttgct ctggcagatt
     1381 tgattggctg attcgctgag tttgaagagg aaggggtcga ggatgccgct tcttgctgtg
     1441 ctgcttgtgc gaccgcagct tgttgagcgg cttgagctgc agctgctgct tgagcggctt
     1501 gttttgcttg ttcttcagct gcttttttcg cttcttctgc ttttttcttt tcctcttctt
     1561 cttttttctc tttatcagtg agcatcttat ctacttgttt caattgttca ttcaactgag
     1621 cagatagatc tgtatttttc actttttcta ctgcttcttt tgccgtcttg tattggtcgc
     1681 gtgttgctga atccactact tttccgtctt taaagactat agctgttttt tctttagctg
     1741 tttgcaattc agcatattga tttttagctt ctgtttgtgc ttgttgaatc aattttccaa
     1801 aatctgtgtt atccgtttcg attgcttgga tctctttatc tgctttcaac ttgatgtcat
     1861 ctgctagatg atcatcgacg atggcaggtg ctatgaacca ctcattcact ttttggatat
     1921 ctcggatctt agattggata tcttggtaag ttgtttctaa ctctgaatat cctttttcat
     1981 tcttaagact ggataaatca ttttttagtt ttgataaatc ttgatgaatc atatctgcac
     2041 gaataaattg atggtgatca tctgtataaa aggcagcaag tttctcatga agttcttcca
     2101 tttgttgctg tctttttgct tctgctgcta cttcggcttc tactttttga tgatggtcat
     2161 aataagccca gcttcctcca ccgagtagaa gtactgctgc taaaacgaga taaggtactt
     2221 tttttgattt tttcttttgc gctttatctg gatctgtttt ttctaagttt gattcttgat
     2281 gtgttttttc agtatctggc acgattgtta ttgatttcgc ttctgcttca aacagcttag
     2341 gctggttctc ttctttttct atttttgctt caagatcagt accctctttg cttacagatt
     2401 gattttcagc atcggattca ctatttattt catcgggctc tgttgacttt ttgtctgtgt
     2461 tttcgagtat ttcttttttc tctgcttccg gcagtttatt cgttttttcc ttttctaatt
     2521 gtttttcagt aaatacttca gttttttctg tgatatccgt ttttacgtct tttattttta
     2581 ctgtttcttt acctgtggcg ttttctctat cagagtcttt ttcagtttct tgttcctttt
     2641 ctggctctac tttgttttcc tgtgcaattt tttcactgat cattttgagc agctctttcg
     2701 cgtgctcttc ttttgtatct tcacgatgtg ctttgatata agcggatagt atcgaatttt
     2761 cttcctcatt atcgacgatt tctttcagtt cagctactct tcttttttgt tttgattcaa
     2821 gcgtttcttc ttgattcttt cctgattctt ccgaagtctc atgaacagtt gctttcttct
     2881 gttcattttt ctctatatta gttacttgat cgtgagcagg tggttcttcg tcttctggtt
     2941 ctgctttttc gaataattcc attacagatt ctagaggtaa atctttcaat tccgaccatt
     3001 cgattggatc gtttaattct gtatcaggaa agactgcatc ttcagttgat ttatcctgca
     3061 tagctgcatc tgtatttgtt tcttcaattg attcttttat ttgctgatct tctgcagtta
     3121 tttgatgacc gcagtctgga cagatagtat tctgctccat ttttttcgat ccgcaatttg
     3181 gacatttttt catcacttag cgatcggctc ctttactagg tttacgctac catttaatct
     3241 atcatattct atacgtcacg ccaatagctt atttttagga tttacttaaa aataatgaat
     3301 gaatttcctt cctatagaaa aaaaagcttg agactgcgta gtctcaagct ttttttcttt
     3361 taaaataaac caaacagttt tactgatttc tcttctggtc aatagtccac gcttattcta
     3421 agaaatcttt taattgtttt gaacgagaag gatggcgcaa ttttcttaat gctttagcct
     3481 cgatctgacg aatacgttcc cgagtaacac caaatacttt ccctacttct tccaatgttc
     3541 tcgtccggcc atcatctaaa ccaaaacgta agcgaaggac attctcttcc cgatcagtca
     3601 atgtatctag tacatcttcc aattgttctt ttagtaactc ataagctgcg tgttctgctg
     3661 gacttgttgc ttcctggtct tctatgaaat cacctaagtg agagtcatct tcttcaccga
     3721 ttggcgtttc aagggaaaca ggttcttgcg cgattttcaa gatttctcgg actttttctg
     3781 ttggaagatc catttccgca ccgatttctt ctggcgtagg ttctcttcca aggtcttgca
     3841 gcaattgacg ttggatacgt atcaatttat taattgtttc aaccatatga actggaatac
     3901 ggatcgtacg tgcttggtca gcgattgctc gagtgatggc ttgacgaatc caccatgtag
     3961 cataggttga aaacttaaat cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc
     4021 ccatgtttcc ttcttggatc aagtcaagga actgcatgcc tcgtcctaca taacgtttag
     4081 cgatacttac tactaaacga aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc
     4141 cttcttcgat tttcaatgct aacgctactt cttcttccgc agttaaaagg gagacacggc
     4201 caatttcttt cagatacata cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa
     4261 gatcttctgt ttttgcttgt tcagcttttt tctcagcact cttcaagctg tgaattgatg
     4321 gatcaccatt ttcatccacc acactgatac ctgcgtcttc aatcttttgg atcaatttgt
     4381 ccatttcatc tgcattcaaa gtgaatggtg tagcaagctg attggataaa tcatcatata
     4441 caacttgtcg tttgggtttg ttttctttga tgaaagctgc tactgctgct tcatacgttt
     4501 ttttatctgt tccatttgcc ataaaagaag gccccccttg agtagctatt tggcttgttt
     4561 taactgtttc gttaaattga tgatttcaat cgtcaattca agctcaagct gtttgtttcc
     4621 agttcgtctg gcttcttgct gcatttgttt ttttaatgtt atttcatcag ctaagccaga
     4681 tttgctgatc acatctagca agtcttggat ttctcgtggc gaactttctt ctgccaccgg
     4741 taaatattcg atttccacca ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa
     4801 ctctgcaagg ttgaattcat aatgttgtga gacatatgca tcgaataaaa aataaacttc
     4861 tgcatatgca tcatgaataa actgaaattc tcgttctttt aaaatattac ggacagaaac
     4921 ttctttaaat gcacgatata atagaatctg ctctgccttt tctacttgcg tcaaaggtct
     4981 ctttttccgt gcagtttctt gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg
     5041 acgattttgc tgatttgtcc ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc
     5101 gatctgaaac tctgttgaca gttgagatag atacatatct tgttcgattg gtgaaacaac
     5161 tcggctaagt tcaactagta attcttcgag gtattcgatc ttgtcttttt cattatctaa
     5221 atttttccct tgttttaaat aacgtgattt aaaagtaaag actgtttcgc gcccatgagc
     5281 cactaattct tggaaagatg cttctccata cttacggaga tattcatcag gatcaagctt
     5341 ttctggaacg actacgacag aaagtgctaa tcgactgttt tgccgaagca attcaatccc
     5401 tcgattggtt gcttcaaaac ctgcgttgtc tccgtcatag caaaagacca attctttcgt
     5461 cagcttttcc atttgttgga tctgttgagc tgttagactt gtccccatag aagctaatcc
     5521 agttttgata ccagcctgcc atgcagcaat cacatccata aacccttcga acaacaaaat
     5581 ttctccgttt ttccggatat cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt
     5641 attgaacagt tctgtttctg gactattcaa gtatttgggc tgatcttttt cctgcccttt
     5701 ttcaggtgca agccagcgtc ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg
     5761 aaacatcact ctttgataaa aacgatcaga catacggcca tcatcatgcg tgacaaacaa
     5821 tccagagcgt tctaacaatt catttgaaat tgattcattc ttgaatactt gctgaagaaa
     5881 agaacgctca ttaggtgcaa aacctatgtt gaattcttca attaattcta aagacaaccc
     5941 acgactttgc aaatattcta aagctggctg tccagctttt gtatgcaata gcatgtgatg
     6001 atagatttcc gctgcttttt catgagcagc aatcagtttc cccgtagcag attcaggatt
     6061 tcggacagct atagtacgct gacgccattg atcttctaac ggtatctgtt cgaagtctgc
     6121 cacttttacc actgcttctg gaaaacttag accttctagt tcttgaagaa aagtaaacac
     6181 gtttccgcct ttaccacaac caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa
     6241 agacggtgta cgttcttcat gaaaaggaca cagccccaag taatttttac cagacttttt
     6301 caactggaca tattgtccaa tcacctcaac gatattcgtt tgactgcgta ctgtttcgat
     6361 aacctcttga ggaatccgtt gagccatttt ttcaccccct gctctattga tatcttttgg
     6421 gtttaacaga agatatttca ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt
     6481 gcgattttaa aggacacaat tatacatgtt tgattgtatc atatttcatt gctttttcaa
     6541 gtataacaca tttctatctc attaagcatc aatttgctac tcttttcagt aactgatttt
     6601 gaatatcagc cttccagtac aatactcgaa gaacaatatt tacgagcatg aatacaaaga
     6661 agtatcctac gctctctccc aaccctaagt ttacttccaa aattccatgg aacagcattg
     6721 cagctacata tatccctacg atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg
     6781 gataacaagc ttttacaact gccatgcaac tgatcccaat cagcaagtaa tacatcaaag
     6841 tagtagtttc agcatttccc catgcgaatg tttttccaag tagatacacc gcaatgatat
     6901 tcactactac aagcaaggca tttggtaaag cattaataag tgctgtaggc aaataacggt
     6961 aagtgatttt tcttttatct tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg
     7021 ctaaatcaat cagcgtgatt tggataggga taaacggaaa tccaattgct gtaacggcac
     7081 atataatcga taaaataaat gaataaatcg ttttgataaa aaagatgcct gagactttcg
     7141 tcacattgtt gaccactcgg cgtccttcaa acaggacttc tggcaatgta gtaaaatcag
     7201 agtccaacaa taccagattc gcaatctgac gtgttgctcc atcaccttct gccatagcaa
     7261 tgctgcaatc tgcttctctt aacgccaaaa catcattcac accgtcgcct gtcattgcaa
     7321 ccgttctgcc gctttctttc aattcattta ccagcaattt cttttgctgc ggactcactc
     7381 gtccaaaaac cgtatagcga tgagcagctt ctcgcacttc catttcttca gtaatttgag
     7441 aaagatcgac atacgcatca taattcggta atcctgcacg tctagcgata ttggagacag
     7501 taaaaggatt gtctccagaa atcactttca gatccacacc ttcctcatga agataagcta
     7561 gtgtttcttg agcgtttttt cggattggat catcgatttc aaacagagca accggctcaa
     7621 caaatgctgg tttttcttcg ttcaattgct gatttggagc gatccccaac ataagtacgc
     7681 gatagccatt cgcttgggct gtttttagac tgtccggaag ccgatcttct tccaccaaac
     7741 gttcaggcgc tcctacgtac acgactccca gttctggaaa ttccatcgcg ccccatttgc
     7801 gatcagaaga aaatgcgact ttgtttgtca catcataccg attagaaagc tcaaaataat
     7861 ctctcatagc ccgcatagtg atgttgttat cactgctttc tgccagatac gtccccataa
     7921 tttctgggat ggctttctcg tacagcggat gtagtgcttc tacccgctgg atactcattt
     7981 tcccttcagt aatggtccct gttttatcca agcataatgt atcgacatgg gccagcgttt
     8041 caattgaata catgtcttga acaaggattc gtttttttgc cagtttggtt acccctgtcg
     8101 ttaatgcaat acttattagc aataccaaac cctttggaag catgcctaac aaagctgctg
     8161 cagatgcaat aacagaaatt ttgatagaag catcgcggat gaacagtgct tccaaaaata
     8221 gtatgatccc caaaggaata atcacgaaac tagtgaattt ggatactttg cgaatcgaat
     8281 ctacaagttc agaacgaata gctttgtgag tctttgcctc gctagtcaag cggacagcat
     8341 aattatctgc acctactcgg ataacttcag ccatgacaga accgcttgtc aaaaagcttc
     8401 cagaaagcaa ttcagctcca gtttctttga caatcaaatc agattcgcct gtcaaaagag
     8461 cttcatttgc ttctgcccgt ccagatataa cctgtaaatc ggctggtatc tgttctcctg
     8521 ctccgatttt taccaaatct cctaatacca gctctttagt agaaatcgat tgttctttac
     8581 cgtcccggat cactagtact tctgattggg aaacaatcga taatttccgt acaagatttc
     8641 ttgcatgtat ttcttgataa attccaataa cgatattgac gacaataatt gctaaatacg
     8701 ccatgttgga atacgcacct acaaataaca gacaaatccc aataccaaag ttcagaaaat
     8761 tgaataacgt caatatatta tcgctgaata tttgttttgt actttttgat gcatcttcct
     8821 ggtagtcatt ttgctggcca gaagccattt tctgaactac ctcttgctgt gtaagtccat
     8881 tcattttctt tttctccatt ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag
     8941 cgtgttccaa gattaaaaac aacttgtttt tataaaattt atagaaaaac acacgaatga
     9001 ttttacaaaa aagatgccaa cctttttgtt tgaatcaccc gtgtgctgtt tttattatca
     9061 atctatcttt acttatattg ttcctccaaa cggctcatcc accaacctag tgctgtaccg
     9121 gctataaaaa gaacaggaca agctagtacc ccagcaactc ccaatcccgc gtaatcggta
     9181 ggaatgatca taatcgttga agcaaaaact acacctaaaa tgaaatggaa tagcttgcta
     9241 tatgcctttg agaaaatgta atccatgatt tttgacaatc caagaacaca aagaatccca
     9301 ccaattccga tcggcacaat cacttcaagt cgtacttctt taaatccgtc agccatcgct
     9361 ttatacaatc ccatatacac caaaaaatta gaagggctca atcctgggac gatcataccc
     9421 aaaccaatca atccgccggc aatcagccat gttccaaaat tctgatcgac ttgcccgtca
     9481 aataattgag cgccgtataa taaaaacaaa taagccagaa caaaagtaac aaccatgatg
     9541 atcacatcag aagtatctct tccttttttc cctgcttctt tccataacgc aggaacagtt
     9601 ccagcgatac atccgacaaa aaaccataat atcgttgttt catacgcacc taataaaaaa
     9661 ctgactaaaa aagaaagtag aaagatgcct gtcactccgc ctagaccaac aggcagaaaa
     9721 aacaaaacat tttctttgaa attttttgta aggtgggcta aaaaagaaat cagccgctca
     9781 tacatgccaa agacagctgc taatgcacca ccgctgactc ctggcaagat aaatcctgaa
     9841 ccgataaaca ttcccttcac aaaccgtaac atccagtctt tggttgtgta ccccacttct
     9901 tgattttcca ttttttgcct tttttccact atttgcgtac tccttctttc atttaatttt
     9961 attcatgatt gaacatttcc tgctatttta cttaaagact ttttcatagg tctctattat
    10021 tttttcattc attttgacaa tatcaggata aaacatgtta cctccattat gatagagata
    10081 gccgccattg taaagcaagg actggatccg ccaatatcgg tattgtttcc cttcctcatt
    10141 tcctaaaagt gggctcagca catctttaga atacgcttct gctaattcta ctgtgttttc
    10201 tccgccatga ttttttacat aggcaatata atctttccca aaattatatg cttggacagc
    10261 tgtccataaa tcgcatcctt gtttctttgc ttctactatc atttccgcca aatatttgac
    10321 accttgatca atactgtcag ctggattatc aatgatctca gcttctccat gcagactttc
    10381 cgagctctgc atgagatctg tcccttttcc ttttgattcg gtcaatatta tcgccttgat
    10441 caaattctcg tagttttcca tatgataatt ttttgcagct gttgttattt ctatttgata
    10501 gctttccact cgttttaccg aacgataagt tcttcctcat aaaaacaaca ccgcaccaat
    10561 aaccgtcaga atgatcatgt aaaaaagaat acggataata gataatcgtt ttcgcttcat
    10621 ttcatcacct agttattttg acatcttttt ttatcttact atttctttat cttcttggct
    10681 atttcttcct tgatttctgt gtacgcctta agaaaaaatg aattttcctg tattttttga
    10741 catcttacga aaatgatcgt tgtttttcag taaaaacaat tgcccaatga ctgatataat
    10801 cgttatactt tacacagtag gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc
    10861 agatgggagc gaaatttact atgaaaagag cggtcaaggc ttccctctct ttttactgca
    10921 tggcaatgac ggcagcggcc gctttttttc agaacaggtc cctgtactcg aacgctacta
    10981 taccgtgtat ctagtagata gccgaggaca tggtcgttcg acaaacgaag catctatgtt
    11041 gaattttcag ttaatggccg aggatctgaa tacgatcatg ttgctggaaa aaatcgatca
    11101 ggcagatttt cttggattta gtgatggagc aaatctagca ctggtatttg ccagcagctt
    11161 ccctaaaaaa gttcatcgat tgattttaaa ttctggaaac acgcttgtta aaggcgttcg
    11221 tttttctgcc agagtcatca gtaatattca ttatgcatgg gtatggttac tctctttatt
    11281 tcgcccaagt cttcgcaaaa atcttttagt tatcaaactt ttgctgcatg acatcgggct
    11341 tacagaaaat gatttaaaaa aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga
    11401 tgtaatcaag ctaaaacatt ctttgtatat agctaaaact ataccaaaag cttcctttgt
    11461 gcttgtgaaa gaacaaggac atgaattagc tagaaaagat cctgaacgct tcaatcgaga
    11521 agttcttcaa tttctttctg aaacataact attaggaata gaaggtgatt tatttgttaa
    11581 aaaataccat acaatggctg aaaaaccacc taggactgtt caaaacaatc tttttgattt
    11641 ctgtcattgt cattattgtc ggtgaattga tgtccatcgg taaaactcta tctatccagc
    11701 aattaggaga aacgtttgcc actatacctg tatggaaaag cggactaatg ctattgattg
    11761 gtcttatctc tgtgttacct atggttggtt atgatattat tttgaatcgg atgcttgaac
    11821 aaaagcagaa tcctcgttac ctgtttgaga caagctggct gatcaatacg atcaacaata
    11881 ttgccggttt cggcgggttt gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga
    11941 aagagagcag aaacgtgatc caagcattat cgaaagtttt cttgtttttg atggctggac
    12001 tttctattta cagtctgctt tcttttttac ttgtagtctt cacgccagtt gctccttttt
    12061 tgaagcaata ttggatatgg ttgatcggcg gcggtctata ttttcctgtc gtctttttat
    12121 ttactacttt caaaaagaaa ggattccttg gtggtacctc acttaaaact caaggacagc
    12181 tattgctagt ttcattgttg gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt
    12241 atttaatgga aatccggatt gatctgtggc aaacaattcc attattcatt gctgcttcag
    12301 tgataggaat cgtgtctatg attccaggag aaattgggag tttcgatgta atgatgatta
    12361 tcgggttgtc cgctattggt gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc
    12421 gcctgttcta ctacatcatt ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg
    12481 gttccacttt tgatcagcgt tattctggta tccctaaaca attagcaaca gaaattgcgc
    12541 ataaaatcgt ggttgtctta ttatactttt ctggaatcat gctagtactt tctgccacta
    12601 tcccgcaagc ttttacagag tttcgctggc ttcatagttt gaacccattg aaatttcatt
    12661 ttattatcca atttcctagt atactattgg gatttctttt aatcattatg ggcagaggga
    12721 tcgcagcacg tgtgaaacga gcatatttgc ctacaatctt cttgattgtt ctagctttac
    12781 tttatgtttt attaagtgat tttagtttca caccggtggt tttcttgtct attctgcttc
    12841 tgatcattct tgcttctaaa aacgaattat ttcgggaaca attgatctat tcatgggagt
    12901 ggcgtacgat cgatggaatc attattggtg cactgtcctt gctttacatc gttattgggg
    12961 tatacaatct gccggatttc cctcatcggc gtcaccattt tatttccttt ttcttatttc
    13021 cttctgaaaa gatttggttt tcgggattgt tggccatcat cgctgttagt ttcatgatcg
    13081 ttctatttgt tcatttcctt caaggagaaa agaaacagat tggcgaagcc tttaatgaag
    13141 aaaaggccct gaagatcctt acaacttatg gaggaaactc cgatagtcaa ttgatcttct
    13201 taaaagataa gcggatgttt gcctatgaaa aagacggtga accaactgtt cttctccaat
    13261 tcgcttgctt caataataaa tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt
    13321 ttccagaagc aatcgaagct tttatcgaag aaacagatcg actatgctat ttaccagttt
    13381 tctacgaaac aagcgaagag attgttatga ttttgcatga attcggctac gatttcatta
    13441 agatggggga agaagcttat gtagacctga atagttttac aacttcaggg aaaaaaatga
    13501 agggcactcg tgctgtcttg aatcggatcg aacgagaagg atttacattt gatgtcttac
    13561 aaccaccatt ttctactgag caaatgagta ctttcaaaaa tatctctgat aattggttgg
    13621 gttcacgaaa ggaaaaaggg ttttctttag gctttttctc tgaagattat ctacaaagag
    13681 cgcctattgc agttgtcaaa aatacccaag aagaaattgt cgcttttgca acgatcatgc
    13741 ctacttacac gaacaataaa gtaggaacga ttgatctgat gcgatatgat tctaaaaaag
    13801 caccttctgg cagtatggat ttcttgttct tgcatttgtt tgcctatatg aaagaagaac
    13861 atatccaatg gttcaattta gggatggccc ctctttcaaa tgtcggaact tccagaaaga
    13921 gttttttaca ggaaaggatt gcatacttgg tttacgagtt tggttctcat ttttattctt
    13981 tccatggatt aagagaatat aaaagcaagt acgctacaaa ctgggtctca cgttatactc
    14041 tatattctag agatagttgg attgcatatg ttatgattgc tttgctgatt attgataatg
    14101 cgccagttga gcagacacaa ggtagaataa gtggtttgaa aaaatggata cgaagaaagt
    14161 attaaaaact gcataccgat agatgaacac tctcatgatg aataaaagag actgaagtaa
    14221 aacgaaagtt catgcttcta caatctcttc ttatacaatt gaagaacaaa gagcggtcca
    14281 aacagaaatc ctgtttggac cgctctttta ttttaaacat ttttcattac caaaatattc
    14341 gagtcgcgtt tcttatgctt cgatctctgt aacgatacct gaacctactg tccgtccgcc
    14401 ttcacgaatt gagaatgtcg ttccgttttc aacagcgatt ggatggatca agtcaacatc
    14461 aatcgtcacg ttgtcaccag gcatgaccat ttctgtatct tctggcaatg tgattgttcc
    14521 tgtaacatca gttgtgcgga agtagaactg tggacgatag ttgttgaaga atggagtatg
    14581 acgtccacct tcttcttttg tcaacacgta cacttctgct ttgaattttg tatgtggagt
    14641 aatggaacct ggtttagcaa gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg
    14701 taacaacacg cctacgttat ccccagcttc cccgtaatct aacgttttac ggaacatttc
    14761 tacacctgtt acaactgctt tttgtgtttc aggtttgatc cctacgatct ctacctcatc
    14821 accgacacga acagcaccac gatcaatacg acctgaggca acagttcctc gacctgtaat
    14881 tgagaagacg tcttccactg gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg
    14941 gatatattca tctactgtat ccatcaattc cataatagca gcttcagcat ctggatcgcc
    15001 ttgcaatgct tttaatgcgg aacctttgat cacaggagtg tcatcgcctg ggaaaccata
    15061 ttcgctcaat aactcgcgaa cttccatttc taccaaatcg atcaattctt catcatcgac
    15121 taaatcaact ttgttcaaga aaacaatcag gtattttaca ccaacttggc gtgacaataa
    15181 aatgtgttca cgtgtttgag gcatcggacc gtcagttgca gaaacaacta agattgcacc
    15241 atccatttgt gctgcccctg taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc
    15301 aatatgcgcg tagtggcgtt tttctgtttc atattctacg tgtgcggtat tgatcgtaat
    15361 cccacgttca cgttcttctg gagctgcatc aatactagca tagtcttgag gatttgctag
    15421 gccttttttg cctaatactg tagtgatcgc agcagttaaa gtagttttcc catggtcaac
    15481 gtgtccaatc gtaccaatgt taacgtgtgg tttacttctg tcataatgtt cttttgccat
    15541 aatttacaaa cctcctaaat aatatagatc actgaaaaat ttcagctctc acctttttat
    15601 tatagtcttt ttttgactaa atcaaaaaga atacgtccga cttacttttt gtaagattcg
    15661 aactaagctt attatacgtc aatattggtc aaagtcaact aagagcactc gtctgtctta
    15721 tacgcgaaaa aagtaccttt tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga
    15781 tagtccttaa ctttcttttg aagcaataag aacaaaatga ggaaaagttt aaaattcagc
    15841 tgttgccaac aactttcaat agacgaaatc ttctatttcg ttaataataa ggaaagaggt
    15901 gaagtacatg aaaaaattaa ttggcgtact tgtcgtttta ggcatatttc tcggcggaag
    15961 ttttgcagct tatcattatt tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa
    16021 tggcgaaatg tcagctaccc aagcagataa cggagagaaa ttcacgactt atacctacaa
    16081 gcaagcggct tataataaag atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga
    16141 acatccatta aagatgaatg cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt
    16201 aagctgggaa gaaataaagg cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa
    16261 ttagtaaatg gtcttaaaat tttattagat aaaa
//
LOCUS       DBKOIJ010000054        16086 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000054 DBKOIJ010000000
VERSION     DBKOIJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.055"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            226..894
                     /locus_tag="ACV5NJ_001354"
     CDS             226..894
                     /locus_tag="ACV5NJ_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HHY6485453.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACV5NJ_001355"
     CDS             complement(1094..1414)
                     /locus_tag="ACV5NJ_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485454.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACV5NJ_001356"
     CDS             complement(1451..2833)
                     /locus_tag="ACV5NJ_001356"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HHY6485455.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACV5NJ_001357"
     CDS             complement(2989..4176)
                     /locus_tag="ACV5NJ_001357"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HHY6485456.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACV5NJ_001358"
     CDS             complement(4183..5214)
                     /locus_tag="ACV5NJ_001358"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HHY6485457.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACV5NJ_001359"
     CDS             complement(5230..6684)
                     /locus_tag="ACV5NJ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HHY6485458.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACV5NJ_001360"
     CDS             6859..7764
                     /locus_tag="ACV5NJ_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HHY6485459.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACV5NJ_001361"
     CDS             7855..8532
                     /locus_tag="ACV5NJ_001361"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HHY6485460.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACV5NJ_001362"
     CDS             8529..8699
                     /locus_tag="ACV5NJ_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485461.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACV5NJ_001363"
     CDS             8813..9709
                     /locus_tag="ACV5NJ_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HHY6485462.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACV5NJ_001364"
     CDS             complement(9764..11134)
                     /locus_tag="ACV5NJ_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHY6485463.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACV5NJ_001365"
     CDS             11372..12118
                     /locus_tag="ACV5NJ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HHY6485464.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACV5NJ_001366"
     CDS             12115..12954
                     /locus_tag="ACV5NJ_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HHY6485465.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACV5NJ_001367"
     CDS             12955..13905
                     /locus_tag="ACV5NJ_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HHY6485466.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACV5NJ_001368"
     CDS             14046..14798
                     /locus_tag="ACV5NJ_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485467.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACV5NJ_001369"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACV5NJ_001369"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HHY6485468.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5217 a         3004 c         3233 g         4632 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgct
//
LOCUS       DBKOIJ010000055        15922 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000055 DBKOIJ010000000
VERSION     DBKOIJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.056"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            291..1430
                     /locus_tag="ACV5NJ_001370"
     CDS             291..1430
                     /locus_tag="ACV5NJ_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HHY6485469.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACV5NJ_001371"
     CDS             complement(1497..2072)
                     /locus_tag="ACV5NJ_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHY6485470.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACV5NJ_001372"
     CDS             complement(2077..3051)
                     /locus_tag="ACV5NJ_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HHY6485471.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACV5NJ_001373"
     CDS             3334..4302
                     /locus_tag="ACV5NJ_001373"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHY6485472.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACV5NJ_001374"
     CDS             4415..4693
                     /locus_tag="ACV5NJ_001374"
                     /EC_number="2.3.-.-"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6485473.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACV5NJ_001375"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACV5NJ_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HHY6485474.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACV5NJ_001376"
     CDS             5150..5905
                     /locus_tag="ACV5NJ_001376"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHY6485475.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACV5NJ_001377"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACV5NJ_001377"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HHY6485476.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACV5NJ_001378"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACV5NJ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HHY6485477.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACV5NJ_001379"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACV5NJ_001379"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HHY6485478.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACV5NJ_001380"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACV5NJ_001380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HHY6485479.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACV5NJ_001381"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACV5NJ_001381"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HHY6485480.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACV5NJ_001382"
     CDS             10642..11184
                     /locus_tag="ACV5NJ_001382"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HHY6485481.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACV5NJ_001383"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACV5NJ_001383"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HHY6485482.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACV5NJ_001384"
     CDS             12777..13679
                     /locus_tag="ACV5NJ_001384"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HHY6485483.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACV5NJ_001385"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACV5NJ_001385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HHY6485484.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACV5NJ_001386"
     CDS             15122..15544
                     /locus_tag="ACV5NJ_001386"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HHY6485485.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5261 a         2874 c         3442 g         4345 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat at
//
LOCUS       DBKOIJ010000056        15743 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000056 DBKOIJ010000000
VERSION     DBKOIJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.057"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(24..722)
                     /locus_tag="ACV5NJ_001387"
     CDS             complement(24..722)
                     /locus_tag="ACV5NJ_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HHY6485486.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACV5NJ_001388"
     CDS             complement(715..2067)
                     /locus_tag="ACV5NJ_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HHY6485487.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACV5NJ_001389"
     CDS             complement(2290..3690)
                     /locus_tag="ACV5NJ_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HHY6485488.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACV5NJ_001390"
     CDS             3851..4588
                     /locus_tag="ACV5NJ_001390"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HHY6485489.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACV5NJ_001391"
     CDS             4713..5192
                     /locus_tag="ACV5NJ_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HHY6485490.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACV5NJ_001392"
     CDS             5189..5956
                     /locus_tag="ACV5NJ_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HHY6485491.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACV5NJ_001393"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACV5NJ_001393"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HHY6485492.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACV5NJ_001394"
     CDS             complement(6859..7461)
                     /locus_tag="ACV5NJ_001394"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHY6485493.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACV5NJ_001395"
     CDS             7567..7797
                     /locus_tag="ACV5NJ_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485494.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACV5NJ_001396"
     CDS             7788..8036
                     /locus_tag="ACV5NJ_001396"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6485495.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACV5NJ_001397"
     CDS             8142..8726
                     /locus_tag="ACV5NJ_001397"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HHY6485496.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACV5NJ_001398"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACV5NJ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HHY6485497.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACV5NJ_001399"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACV5NJ_001399"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HHY6485498.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACV5NJ_001400"
     CDS             10627..10806
                     /locus_tag="ACV5NJ_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485499.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACV5NJ_001401"
     CDS             10828..11853
                     /locus_tag="ACV5NJ_001401"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HHY6485500.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACV5NJ_001402"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACV5NJ_001402"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HHY6485501.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACV5NJ_001403"
     CDS             13425..14372
                     /locus_tag="ACV5NJ_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HHY6485502.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACV5NJ_001404"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACV5NJ_001404"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HHY6485503.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACV5NJ_001405"
     CDS             complement(15274..15516)
                     /locus_tag="ACV5NJ_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485504.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACV5NJ_001406"
     tRNA            15637..15709
                     /locus_tag="ACV5NJ_001406"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5350 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tta
//
LOCUS       DBKOIJ010000057        15723 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000057 DBKOIJ010000000
VERSION     DBKOIJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.058"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            32..1459
                     /locus_tag="ACV5NJ_001407"
     CDS             32..1459
                     /locus_tag="ACV5NJ_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6485505.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1551..1916)
                     /locus_tag="ACV5NJ_001408"
     CDS             complement(1551..1916)
                     /locus_tag="ACV5NJ_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HHY6485506.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1913..2278)
                     /locus_tag="ACV5NJ_001409"
     CDS             complement(1913..2278)
                     /locus_tag="ACV5NJ_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HHY6485507.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2470..2718)
                     /locus_tag="ACV5NJ_001410"
     CDS             complement(2470..2718)
                     /locus_tag="ACV5NJ_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485508.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2717..2845
                     /locus_tag="ACV5NJ_001411"
     CDS             2717..2845
                     /locus_tag="ACV5NJ_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HHY6485509.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2902..4596
                     /locus_tag="ACV5NJ_001412"
     CDS             2902..4596
                     /locus_tag="ACV5NJ_001412"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HHY6485510.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4696..4869
                     /locus_tag="ACV5NJ_001413"
     CDS             4696..4869
                     /locus_tag="ACV5NJ_001413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485511.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5247..6380
                     /locus_tag="ACV5NJ_001414"
     CDS             5247..6380
                     /locus_tag="ACV5NJ_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HHY6485512.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6411..6731
                     /locus_tag="ACV5NJ_001415"
     CDS             6411..6731
                     /locus_tag="ACV5NJ_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HHY6485513.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6760..7224)
                     /locus_tag="ACV5NJ_001416"
     CDS             complement(6760..7224)
                     /locus_tag="ACV5NJ_001416"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6485514.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7356..8609)
                     /locus_tag="ACV5NJ_001417"
     CDS             complement(7356..8609)
                     /locus_tag="ACV5NJ_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHY6485515.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8638..8955)
                     /locus_tag="ACV5NJ_001418"
     CDS             complement(8638..8955)
                     /locus_tag="ACV5NJ_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HHY6485516.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8981..9301)
                     /locus_tag="ACV5NJ_001419"
     CDS             complement(8981..9301)
                     /locus_tag="ACV5NJ_001419"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6485517.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9765..11180)
                     /locus_tag="ACV5NJ_001420"
     CDS             complement(9765..11180)
                     /locus_tag="ACV5NJ_001420"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HHY6485518.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11197..12513)
                     /locus_tag="ACV5NJ_001421"
     CDS             complement(11197..12513)
                     /locus_tag="ACV5NJ_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHY6485519.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12663..13361)
                     /locus_tag="ACV5NJ_001422"
     CDS             complement(12663..13361)
                     /locus_tag="ACV5NJ_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HHY6485520.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13650..14606)
                     /locus_tag="ACV5NJ_001423"
     CDS             complement(13650..14606)
                     /locus_tag="ACV5NJ_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHY6485521.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14719..15552)
                     /gene="msrA"
                     /locus_tag="ACV5NJ_001424"
     CDS             complement(14719..15552)
                     /gene="msrA"
                     /locus_tag="ACV5NJ_001424"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HHY6485522.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4889 a         3186 c         2692 g         4956 t
ORIGIN      
        1 gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat
       61 tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa
      121 gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa
      181 aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata
      241 tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt
      301 tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga
      361 atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttattt
      421 taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg
      481 tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga
      541 atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt
      601 ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca
      661 gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat
      721 ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga
      781 agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca
      841 gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat
      901 aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc
      961 gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat
     1021 gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac
     1081 aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat
     1141 gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat
     1201 ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt
     1261 aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa
     1321 gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc
     1381 ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc
     1441 agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg
     1501 aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt
     1561 tggacatcta cgggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact
     1621 accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc
     1681 ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata
     1741 gcattgttct cgctgactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa
     1801 aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt
     1861 cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta
     1921 cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga
     1981 ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa
     2041 ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata taacttgcta
     2101 gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag
     2161 accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac
     2221 aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat
     2281 acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg
     2341 ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc
     2401 tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta
     2461 aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt
     2521 acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct
     2581 atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc
     2641 tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa
     2701 caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct
     2761 tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt
     2821 atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg
     2881 gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt
     2941 gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc
     3001 accttttatt cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg
     3061 gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc
     3121 ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct
     3181 tctgttactg agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac
     3241 cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa
     3301 ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca
     3361 gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt
     3421 tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct
     3481 gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat
     3541 ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc
     3601 gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa
     3661 ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca
     3721 ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat
     3781 gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg
     3841 gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga
     3901 aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg
     3961 acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat
     4021 gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat
     4081 atgtctattg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt
     4141 atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt
     4201 aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct
     4261 ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc
     4321 cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca
     4381 aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct
     4441 tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat
     4501 tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct
     4561 actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc
     4621 ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg
     4681 gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaagct
     4741 gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg
     4801 ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
     4861 ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat
     4921 aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc
     4981 tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat
     5041 ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa acacacctct
     5101 ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat
     5161 caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt
     5221 ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa
     5281 tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta
     5341 tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt
     5401 tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc
     5461 aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc
     5521 attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt
     5581 tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg
     5641 aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc
     5701 ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa
     5761 acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat
     5821 tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac
     5881 agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca
     5941 cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg
     6001 attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct
     6061 tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa
     6121 cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt
     6181 ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa
     6241 tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc
     6301 cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa
     6361 aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa
     6421 tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac
     6481 gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat
     6541 taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat
     6601 atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa
     6661 tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa
     6721 aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg
     6781 atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta
     6841 tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg
     6901 catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg
     6961 ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc
     7021 aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg
     7081 aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac
     7141 tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa
     7201 tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa
     7261 aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac
     7321 ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt
     7381 ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa
     7441 ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt
     7501 ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca
     7561 aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg
     7621 ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca
     7681 atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct
     7741 aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt
     7801 ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg
     7861 gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag
     7921 aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt
     7981 cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat
     8041 gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat
     8101 gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt
     8161 tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata
     8221 aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa
     8281 atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag
     8341 aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct
     8401 ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag
     8461 ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag
     8521 tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt
     8581 ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta
     8641 cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa aagctataga
     8701 tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt gatttccttg
     8761 tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc
     8821 atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc
     8881 cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt
     8941 tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt
     9001 ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata ttgattacat
     9061 ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt
     9121 gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag
     9181 cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg
     9241 tgactaggag acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca
     9301 ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt
     9361 aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca
     9421 ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt tttcttttaa
     9481 cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat aaacggtatt
     9541 caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga tgttgttttt
     9601 actttgctat tcttattact tgatgcagga cagctttttc acaacctctc aataaacggt
     9661 gttcaatcag ctgaccctgg gtattagctc aaaacgctgg attcacaacc tcttttattt
     9721 caaataacca gtaaatacaa tgaacatcta caaaaaactt ctatttattt atagatttcg
     9781 tcaggcaaat ccaattctct cttatcaatc aaagtctgat accacgaagc agattttttc
     9841 aatgttctgc ttcgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat
     9901 gcattcattg gtgacacaca gtcagtgaat gcccataaca tatagccttc gcaatttgcg
     9961 ccttcttctg ttgctttgat caagtaatgc aaatgctgtc cgataaattc gatacggtaa
    10021 tcatcttgca cgattccttt ctcatccata tactgttctt catattctct tcccataccg
    10081 ttttctgtta tcagccaagg gatattatga taattttctt ttagatacat ggctatatca
    10141 tacatgattt tcggatagat ttcccatccc ctggaattat tcattttctt cccaggtaaa
    10201 tcaaagactt cataatatcg ttcaggatgg aaaggtgtct cttcattcca ttgataacga
    10261 ggggcgcgaa cgcgtttagg ataatattga ttgattccaa ggaaatctac tgtatgatgc
    10321 tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt ttgacacaac
    10381 tccaggaact cttctgtgta gtatcctttt accatcggat caaaaaatac tcgattataa
    10441 aacaaatcat acatataagc agctttttgg tcttctggag aattggatcg agcatagacc
    10501 atttcaggat ttaaaataca gccaatcctt ccaccataat ttccgctgcg gtaaatctct
    10561 actgctttag ccgttgctaa aactttatga taattccaca tcatccactt ttttgtgttt
    10621 tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg
    10681 ttgaatgtaa accagttttt taccctatca ccgtaacgat caaaggcaat ttgagcatac
    10741 ttagcataaa aatcaactac ttttcttgaa gaccagccat catatttttc caaaagaaaa
    10801 gctgggactt cataatgttc caaacaaatc atcggttcta cgtctgcttc gatcaaagag
    10861 tcgatcattt cacttatatg ctgagcatat tcttcatcta ctatacagtt ctcataatca
    10921 gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc
    10981 agatccacat cctgctggta ttgttccata aaattagttg ctacggctgg gccataccca
    11041 ttatgccaaa caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct
    11101 ttttttcctt tccaaccttc cgtttgccaa gcagaagatg cagcaccaag aataaaattt
    11161 tttggaatag taattttcat gatttcctcc tgttttttag atcccatcat tttccacacg
    11221 atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac aaacaagttg
    11281 cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga tcggcggcat
    11341 tggccaaggg accattacaa ctgctttcgc cgcaaatcct aaagcggttg caaaataacc
    11401 aatcgttcca gtgataagag gcgttaagat aaacggaata gctaaaattg gattcagcat
    11461 gatcggcata ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagggataa
    11521 cttggtgatt tgtttcatat cttcccgttt tccaactaac aatactgcaa tcagtaaacc
    11581 gatcgtgact ccagacccgc ccatactcat atacatatcc caaaatggca tagtaataat
    11641 gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag
    11701 caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca cccaaaagac
    11761 ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgtacgacac cttctaatgg
    11821 gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt ctttgatgat
    11881 aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag cagaaaaact
    11941 agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct tctgtttcct
    12001 caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa ataacccttt
    12061 cgtatctgta tattgtttag ccaatacgtt ttctactata acagtctgat tttcattcac
    12121 taattcataa acagttggat taacagataa ataagacatc actgcgatga taccagggaa
    12181 tacaccatcc actttattca gcttggctaa ttcgatccct atcagaaaaa cagcatagat
    12241 ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta attcttctaa
    12301 aaaacgcaag aaacttatct gtgctaagcc attttccgag tcaaagacca ttgctgaaaa
    12361 caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat
    12421 caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa
    12481 tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt gacgtcatct
    12541 ctttttgtct attagtcagc gcttacattt cgatgaacag aaactattta ctcctctttt
    12601 ttaccaaata taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac
    12661 tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt tattgccata
    12721 tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc
    12781 tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt tttattaaac
    12841 aaagcaattg attcattaat gattgggata atatgcagca aggctacgtt tccgctagct
    12901 tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt gccttctcga
    12961 aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt cttcgttgca
    13021 tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc aaataagtct
    13081 cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct
    13141 cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt cctggaagct
    13201 agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc ggacaattca
    13261 tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata tctaaggatc
    13321 ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca
    13381 tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat
    13441 acaaaattga ttaatccgtt catagacata caaacgaaga agatatttac cataaaaaag
    13501 actaggataa aattcactcc tagccttcga tcatttttat tttgaacgac aaaaaatatg
    13561 atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt ttttttaata
    13621 tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat tctcaattct
    13681 tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa ctgacttaac
    13741 cgacttgctc cgaccagtac acttgtaaca gatttttgtc gcaaattcca tgcaagggcc
    13801 atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt gattttttcc
    13861 agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg tgctctagaa
    13921 tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag cggactgaac
    13981 gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc tagtagtgca
    14041 cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt tctttttaaa
    14101 atttcagtca ttttttttgt atcttctcca ttatagttgg atataccaat atataatgct
    14161 tttccttgtc ttacaagttg catcaaagct tctgctgttt cttccaaagg tgtatcaggg
    14221 tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg gagactttgg
    14281 tcacaacttg aaatgatgga tttacgagag ccccattccc catatggccc ttcccacata
    14341 tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta
    14401 aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg
    14461 tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata
    14521 ggatcataat caccaaaatt ttgccataat ccgagagata aaatcggtaa tttcaaacct
    14581 gaatgaccgg atttacgata ttccattttt tcataacgtg cactatctgc aatatacaat
    14641 ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt tttctatttt tagtttacta
    14701 cattttaaag aaaaggagtt aatcaaaatg attttttcgt atattttttt gccatttgtt
    14761 ttttactcgt tgatagatca gaaattgatt acgtgcttgc tggatttttt tataacgttt
    14821 cggttgtttt ttgtaaaatt gctgatgata gttttctgct ggccaaaacg taccggctgg
    14881 ttcgattttt gtgacgattg gttggtggaa agttcctgaa ctgtctagtt tctgttttga
    14941 ttgttccgct aattcttttt gtcgttcgac ttgataaaaa atcactggac gatattgctt
    15001 ccctctatct tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc aatagattgt
    15061 cagcaactcg ctataactta tttctcttgt gtcataaatg atctccacag cttccacatg
    15121 acctgtgtaa ccaccactta cttgatcata atttggtttt tcaacatgtc cgcccgtgta
    15181 tcctgacaaa acagatagga tccctttttt cgattcaaac ggctcgacca tacaccagaa
    15241 acatccccct gcaaatactg cacgttcttg atagtcagca tcttgcatgg catgcttgga
    15301 aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc aataaataaa agtccattac
    15361 atcaggcgtc agattcattc ggatagctaa tggacgtagt gttgcttcta gttttgataa
    15421 ctgttcttta acggatatat tttctcttat gtcattcttt gtctgaatca atacttttcg
    15481 ttcccaatct cgtgtattcg gattcaaaat cagattataa agtgtttgaa tgaatatttg
    15541 ttcctcatcc atagattctc ctcctttccg aagaagacaa gctagtgact ttagctgtaa
    15601 tttatcataa agtgaggaaa taagcgatta cttcatcccc ctacagattt ttcgtataaa
    15661 aaagagagaa tccttatcga cagattctcc tttttaatca atttatttta taaatgaaaa
    15721 gtt
//
LOCUS       DBKOIJ010000058        15300 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000058 DBKOIJ010000000
VERSION     DBKOIJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.060"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            141..632
                     /locus_tag="ACV5NJ_001425"
     CDS             141..632
                     /locus_tag="ACV5NJ_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HHY6485523.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(656..3031)
                     /locus_tag="ACV5NJ_001426"
     CDS             complement(656..3031)
                     /locus_tag="ACV5NJ_001426"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HHY6485524.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            3357..3983
                     /locus_tag="ACV5NJ_001427"
     CDS             3357..3983
                     /locus_tag="ACV5NJ_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HHY6485525.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            4006..4710
                     /locus_tag="ACV5NJ_001428"
     CDS             4006..4710
                     /locus_tag="ACV5NJ_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HHY6485526.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            4902..5735
                     /locus_tag="ACV5NJ_001429"
     CDS             4902..5735
                     /locus_tag="ACV5NJ_001429"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HHY6485527.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            5932..7860
                     /locus_tag="ACV5NJ_001430"
     CDS             5932..7860
                     /locus_tag="ACV5NJ_001430"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HHY6485528.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            7998..8453
                     /locus_tag="ACV5NJ_001431"
     CDS             7998..8453
                     /locus_tag="ACV5NJ_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HHY6485529.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            8533..8661
                     /locus_tag="ACV5NJ_001432"
     CDS             8533..8661
                     /locus_tag="ACV5NJ_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485530.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            8923..9192
                     /locus_tag="ACV5NJ_001433"
     CDS             8923..9192
                     /locus_tag="ACV5NJ_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485531.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            9592..9846
                     /gene="rpsT"
                     /locus_tag="ACV5NJ_001434"
     CDS             9592..9846
                     /gene="rpsT"
                     /locus_tag="ACV5NJ_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HHY6485532.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(9935..10981)
                     /gene="holA"
                     /locus_tag="ACV5NJ_001435"
     CDS             complement(9935..10981)
                     /gene="holA"
                     /locus_tag="ACV5NJ_001435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HHY6485533.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(11019..13292)
                     /locus_tag="ACV5NJ_001436"
     CDS             complement(11019..13292)
                     /locus_tag="ACV5NJ_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HHY6485534.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(13285..13824)
                     /locus_tag="ACV5NJ_001437"
     CDS             complement(13285..13824)
                     /locus_tag="ACV5NJ_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HHY6485535.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(13960..14178)
                     /locus_tag="ACV5NJ_001438"
     CDS             complement(13960..14178)
                     /locus_tag="ACV5NJ_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HHY6485536.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(14175..14420)
                     /locus_tag="ACV5NJ_001439"
     CDS             complement(14175..14420)
                     /locus_tag="ACV5NJ_001439"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HHY6485537.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(14536..15264)
                     /locus_tag="ACV5NJ_001440"
     CDS             complement(14536..15264)
                     /locus_tag="ACV5NJ_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HHY6485538.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         4689 a         2958 c         2730 g         4923 t
ORIGIN      
        1 aagaataaca gtttatctat taaaataaaa tacttcgaac tcaaatgaga agaattttag
       61 caaacaggta agaatctgcc ctaatcctat gataagatag atgtatggta aatatttata
      121 tgtatatcag gagggttcct atggcaaaag atgctagctt cgacattgta tcagaagtca
      181 atacagaaga agtaaaaaat gcgattcagc aaacaacaaa agaattgtcc aatcgctttg
      241 acttcaagga ttctacagta gaaatcaaat tagagaacag caatctagtt gtattgagcg
      301 atgatgattt caaaatcgaa cagatcaaag atgtattatt tggtaagctg atcaaaagaa
      361 atgtgccaat caaaaatatc catttttccg aatcagaaca cgctctaggt ggcaaagctc
      421 gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa cgcgaagaaa atcactacag
      481 ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca agaagatcag attagagtga
      541 caggtaagaa ccgtgatgat ctacaagctg tgattgcttt attacgtaaa cttgacttac
      601 caatcgagtt gcaattcact aactatcgtt aatcaaaaaa cgaggctgac gtctgtcatt
      661 cctcgttttt ttgatttact gcttcttttg ataaccaaaa acgctgtatt ccttcttcca
      721 attccccaac acttcgtact tctcttgctg tttgccaatg ttgtgggaat aaacgtcgat
      781 aagaaaaact agaaaatcgt tgatctgcca atacaacgat tccttgatcc gccatgccac
      841 gaatcactcg accaccagct tgcaagactt tgttcattcc aggaagctga taagcgtaag
      901 caaatccaag gtgttccttc tcatcataat atgatttgat cagttcctgc tcatgattca
      961 tctgcggcaa acctacacct acgatcatac tgccgatcaa tcggtcccct ctcaaatcga
     1021 ttccttctga aaaaatcccg ccaagtacgc aaaatccaat caatgtttca gcgggatttg
     1081 tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt catgtccgtt tcttggatta
     1141 gcacattgct gtcgggatat tgctgtctga atgcttccac cgcaagatct aaatattgat
     1201 aagaaggaaa gaaaacgaaa taattcccga cctttccatc agcaaaagca tgaatcgtct
     1261 ctactaattt tgccagactg tgttcccttt ttcgataagt cgtctctagg taatttgcga
     1321 tgaaaacttg ctggtttcct tctgggaaag gactcggcag tttgtaagcc agactttttt
     1381 gtccccccag tgtttcctga taataagaga gtggtgaaaa actagcagaa aaaagaatac
     1441 tgctttttcc tttatccaac gactgctcaa gaaacaagct tggatcaata caaaactctt
     1501 ttactatcag atcgcgatac gttttttcga ctgttgtttc gtagtgatca tcataaaatt
     1561 cactgacctt caaaaaatga agcaaatcaa aataataagg aagcaattgt tcttgtgatg
     1621 ggtgctcagg aaattctgcc agccattctt taattttttc gcttaattga taacctgcct
     1681 ttactaaaga ttcagccggt gctttttgat gatgataatc ccaatgatct tcttttgcta
     1741 tttctcggat cgaatcaaat tcttttaata gtttgttgaa acggcgatga agttttttga
     1801 attcctttgg tatagcttcc ttacttcgtt tcgttttttc ataagatagt tctgccgaat
     1861 acatctctcg ggaacgattc accaaattat gtgcttcgtc aatcaaaaac agatagtcct
     1921 cgtttttttc atcttcaaaa aaacgtctta aataaacagt tggatcaaat aaataattgt
     1981 aatccccaat gattacgtca caccagatac tgacatctaa tgatagttcg aaaggacaaa
     2041 gtgtatgctt tcttgcataa gtttcgatga cttcacgagt gatctgattc tcatgattca
     2101 aaagatccca taaaccttca ttaatccgat tgtaataacc attggcatat gggcactgat
     2161 ctggattaca ggtcgtctca tctaaaaagc agatcttgtc tttcgccgtc aaagtgacac
     2221 tttttacttc agctcctgta tcttttaatg ctgtcatagc atcttcagct acttgtctag
     2281 ttattgtttt agccgttaga taaaagatct tttctccctc ttcttcccct acagctttca
     2341 aagcggggaa taacgtagag attgtttttc ctgttccggt aggtgcttct acaaacaatt
     2401 tctgtttcgt acggatcgtt ttataagcag ctaccgctag ttcacgctga cctttcctat
     2461 actcatcatg ggggaatttc aaagccatca aagaggcatt gcgtacagtg cgccagtttt
     2521 cttgaaaaac aagccattct ttataatctt cagtcagctt tttaaaaaaa gtttctaact
     2581 gctctcttgt ctgatgttca acctttctcg tgatcacttc ttctgccgtc tggaagtaag
     2641 tcaattgcag atttatttct tgtaaatcat gttcttgact atagatatag gcatacaccc
     2701 ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa atcttcaaat cgtggttcgg
     2761 aggttttgat ttcatctata taataaatac cgtctctttt gaaaataccg tctgcacggc
     2821 cttcaacgat caggtaatat tcatctaatt gaactgttgt ttttaaaaag acttcttttt
     2881 catagttttc tcccgcatct ttttggagct tgcggtggat tctcgctcct tctaatgcag
     2941 tatggttact ttttttacga ttgtcgatac ttccacgtcg caaaataaat tctactaagt
     3001 gccgcacaga tatttttgac tgtttgatca tttccaccac ctctttctct ctaagattac
     3061 ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa acataaaaaa acaccctgct
     3121 gattaggcag ggatatagga tatagaattt ggatttttgg atgaatctaa tgtaacaagg
     3181 acatattaaa atgctatgaa aacttacttt ccttctcttt agaatatcca aatacttttc
     3241 atatagtctg ataattcaga taagtaaaga gaaataagta cctgtttttg tttcctagca
     3301 atgaatgcgt tatactaatg gatgaaagaa gttttggcta tcaaggagga atgcacatgc
     3361 atatcgaaag attaaaaaca ggaatgattg aagaaaactg ttatttggtc tacaacgaag
     3421 aagcactttt gatcattgac cctggagaag atgcggaacg aatcaaacaa gaaatcaaaa
     3481 aaacaaacca acagccggta gcgattttgc ttacccatac tcactacgat catatcggtg
     3541 cagtggaacc tttacgcgcg tattatcaaa tcccagttta tgtcagccca ctagagcaaa
     3601 aatggctagg tgatccgatt ttgaatcttt ctggactcgg tagacatgat gatattgctg
     3661 atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa aacttatact ttaggaaaca
     3721 tgtcttttcg cgtcgtccct accccagggc attctatagg aagcgtcagc tttatttttg
     3781 atgattttgt cgtttctggc gacgccctgt ttagaggaag tatcggtaga accgatctgt
     3841 atacagggaa tttagaacaa ttacttcata gtattcggac ccagctgttt gtattacctg
     3901 atgaatttgc agtctatcca ggacatggcg atgcaacgac gattgaacag gaaaaaagga
     3961 caaatccttt tttcaatcgt taaaacgact taggaggaga ataaaatgac agaaactaca
     4021 ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa ttgaacgagt acaaggctgg
     4081 tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc attatttagg tcgcgggtta
     4141 aaaaatgtcg acttttcatt tgcttattct agtgacagtg gtcgtgcaat cgaaactgcc
     4201 cgtattattc taggagaaca ttctaaggga aaagaaattc cttattttat tgaaaaaagg
     4261 attagagaat ggtgtttcgg gtctcttgaa ggtggatatg acatggaaat gtggggtgtg
     4321 atccctcgtg tattgaattt taaaacatat gatgaaatgt ttaccacaga agtttctttt
     4381 gaacaaattg ctaatgctgt atacaactta gataccgctg ggtgggcgga aacttatgaa
     4441 acattgcgag accgtgtatg gacaggattt gaagatatcg cccatcactg cgaaaaaaat
     4501 ggtggtggaa atgttttagt cgtatctcat ggtttgacga tcgccttttt gcttaatctg
     4561 atcgatccta aacagcctgt tcgtgctggg cttttaaatg gcagcgtaac aaaggtcact
     4621 tatgcaaacg gtcgattttc tatccacggc atcaatgata cgtcttatat cgataacggg
     4681 aaaacacata gtcgcgactc gtttctataa tggggatatt ttaattttta ccagagaatc
     4741 ataaaaggca aagtctgaac gagggctttg tcttcttttt tctcttcgtg tacttatctg
     4801 ctttatttct caagtttttc ttaatattaa aaaacagatt gggtttctca aaaaataacg
     4861 gtatactata agatgtatct taattgggaa ggaaggaatt tatgtttttt gcaaatatta
     4921 tcaaagcagt gattctcggt attattgaag gaataacgga atggctgccc atcagtagta
     4981 ctggtcattt gatcctagca gatgaattca tcaaactgga tgccagttca caatttatgt
     5041 cgatgttcaa tgtcgttatc caattagggg cgatcttggc tgtcgtcgtt ctttattttc
     5101 acaagttaaa cccattttct ccaagtaaat caacggtaga aaaaaaagat acgtggacac
     5161 tttggtttaa agtgatcgtt gcttgcatac cagccatcat cattggtttg cctttggatg
     5221 attggttaga agcaaatttc cacaagttct taccagttgc cattgttttg atcgtttacg
     5281 gtattgcctt tattattgtc gaaaaacgca acaaaaacaa aacacctcgt tggacttctc
     5341 tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt attccaagtc ttggcacttg
     5401 ttcccggaac atcccgttct ggcgcaacga tcctaggatc tatcttgatt ggtgcttcgc
     5461 gttttgtcgc tacagagttt tcatttttct tagggattcc tgtcatgttt ggcgcaagcg
     5521 gcttgaagat cgtcaaatat ttagctgaag ggaattcatt caaaatggaa gaaactgttg
     5581 ttcttcttgt aggaacattc gtatcttttg tggtttccgt cttagctatc aaattcttac
     5641 taagctattt gaagaaaaac gactttaccg cttttggctg gtatcgtatt atccttggta
     5701 tcattttgat tggttattgg tttattgcaa tgtagaaaaa accagaacac ttctcttatt
     5761 tgagaaatgt tctggttttt tattttcttt aataataatc attataaata ataaaaacaa
     5821 catcaagctt aatcaccaaa tgataaccat tatcgttctc atttagctgt tattattatc
     5881 aattacattt ttattatgct atccttgttt tgacaaggag gtctaataac gatgactaat
     5941 cagaaaaaag cttttgcagc aacaatcggc tgccttttat ttatgatctt cggttttatc
     6001 ttcgaccgat tctctcttac catcacccca cttttttata tcagtgccat tatcatcggt
     6061 ggatataaac aaacatcgga aggcttgaaa gaattgtgga atgatcggac actgaatgtg
     6121 gatttattga tggctttagc tgctattggt gcctgtttga tcggtaatta ctttgaagga
     6181 gcaatgttga cattcatctt ttgtttaagc ggtgcattag aggaatacac aactaataaa
     6241 agtcaaaaag aaattactgc tttgatgaat cttcaaccac aaaaagccca attattaaaa
     6301 gaaaatggac aaatgcaaga agtagatgtt tctcaactaa aggtggataa cttggtattt
     6361 gtcgctaaag gagcagctgt tccgatagat ggctttttag aaagcacaca ggcaacgatc
     6421 gatgagtctg cattaagcgg tgaatctgtg cctgtagaaa aatatttagg cgatgagatg
     6481 tatgcaggaa cgctgaatca aggaaatccc atcacgattc gagtaacaaa aacgagttcc
     6541 gaaactgtct ttgctaagat tatccaatta gtagaagaag cgcagaatac gccaactcaa
     6601 acagcttcat ttatcgaacg aatcgaaaat aattatgtaa aattgatttt gcttgctgtt
     6661 cctctaatga tccttctccc ccactttttc ctaggttgga gttgggatga aagcttttat
     6721 cgaggaatgg tattattagt tgtggcttct ccttgtgcgc ttgtcgcatc tgctacacca
     6781 gcttctctag ctgctctttc aaattctgca aaaaatggga ttttaatcaa aggcggtatc
     6841 catttagaaa agctttctga attaaaagcg attgcctttg ataaaacagg aacgctcact
     6901 aaaggaaaac cagttgtaac tgacagcttt ttctttgatg aaaaggattt ggcacaacaa
     6961 cttcttgttg ctatggaaca aaaaacgacg catccacttg ctcatgcgat tttgcaatat
     7021 ttcgattgca caatacctga agaaatccaa tacttaccaa tagaagaaat tactggtttt
     7081 ggattacaaa ctacctattt aggtgcgcaa tggaaagttg gcaagcacgc atatgatcct
     7141 gagacgatga taatcagcaa agagattgcg gaaatgatcg aacgtttgga aaatcaaggc
     7201 aaaaccgtga tttatctttc taaagatcag cagttaattg ctgttttagg tctgctagat
     7261 attccaaaag caaataccca acaagtaatt tcctacttta aatcccaaaa catccataca
     7321 agtatgataa ccggcgacca ttctggcaca gcaaaggcca ttgctgaaca agtagggatt
     7381 gaacattact atgcaagctg tactccggaa gaaaaaacac aactagtcaa aaaagagaat
     7441 gaactttacc aagtaaatgc gatggttggt gatggagtca atgatgcgcc tgctttagca
     7501 gccgcttctt taggtgtcgc tatggggcaa gggacagatg ccgctatgga tatcgcagac
     7561 atcgtcttaa tgaaaagcga cttaaataaa tttgtctaca gtcatcggct atctttaaaa
     7621 atgcggcgga tcatcaaaca aaatattatt ttttcgattc ttgttattct cttgttgatt
     7681 gcttctaact tcctgcaatt tttaaatcta cctcttggag ttgtaggaca cgaaggaagc
     7741 acaatattgg ttattctgaa tggcttgcgt ttactaagac ctttacctga tctttctgat
     7801 tcttcaaaaa aatgtaagtc ttgtccacta tacaaagcgc ggctgtcttc tgtcaaataa
     7861 gcaaagtgtt tttttatgat tttgtttatt ttatgaaaaa aacagcttat gaataggtct
     7921 tgaaggatga attcctactt ttgtaacttt ctaaaaacat gttatgattg tttcgaagaa
     7981 agtgaggaat atatttcatg gaaagttggc tttttttagg aattatttta cttgtagcaa
     8041 ttttagctaa aaatcaaagt ctgatgatag cggcagcttt tgttttgatt atgaagctca
     8101 taccagcctc ccgttccttt ttcccttggc ttcaaggcaa aggaatcaat attggggtga
     8161 cgatcatcac agcggctatt ctaatcccta tcgcaacagg agaaatcggt ctacgtgatc
     8221 tattagaagc ctttaaatcc cctattggct gggtagctgt tttttgcggt gggctagtgg
     8281 ctgtcttatc ttccaagggt gtaggattga ttgcttctga tccacaaatc actgttgcac
     8341 ttgttttcgg cacgatttta ggtgttgtct tcttaagagg aatcgccgcc ggaccggtca
     8401 ttgcttctgg tatcacttac tgtatcttac aagtatttgc tttaggaaaa tagaacaaat
     8461 tttgtctatt ttaggagatt cttaacaccc ccaatttact gatccgtgat atactgacaa
     8521 cgaggtgata caatgttcga aaaattaaag tatttcatag gaacaatcgg ctggtttctt
     8581 ctagcggtac ttttgattct gggcttagct tatctattat ttcctgattt gatacaaaat
     8641 gtgtttgata ctgctttttg attgacctat taaaacgata taggggatgc tcctgcgatg
     8701 caggggcttt ttttaatcaa tgttttgtaa taatttaggc cgtgccaatg agacttccta
     8761 tagaattcga tgctcttttt tctttttggc tcttctgctc ttttttgtta tactagatta
     8821 cagtggacaa aagaaagaat gatcgcatcc acttcaacga atatcaaaat ggaagaatta
     8881 gtgcctgttt tcaggttact ttgaaaagaa aggaagacgc ttgtggaaac ttttgaaggt
     8941 cttttaattg atacgtacgt tttacaagaa cagccaatga tgctgactgg gatcaacttg
     9001 cagcttgagg accgtgtgat caatggcctg cttaaagatc gctatttggc aaaatatatc
     9061 ttacgtttgc ccctaaataa atatgtgctg gaagtaagtg gcaaatggaa ccgtcggaac
     9121 cagttggtca taaaaaaaat gttgataaaa aatatcgatg aatacattaa aataatcggt
     9181 acaccagaat agcctttact gatcaaagga tactggttat aaaataactg cagtttctta
     9241 atagaaaaac aaagaacaac agggaatcgt atcccttttc aaaaaaaact ttgcaaacgg
     9301 ttctctttgt tatataataa cttagtacca aacattccca ttcaaacttt gtttaaatag
     9361 ataaataaaa atctagtttg gtttgacgct attgcctccc acaatagcgt ctttttgatt
     9421 tttttcagct tgtactatcc gtttttactg aaaaaaaatc ctaataaaag actgttaagg
     9481 aaaaaaactt gtcatgttca tattgcaaag cacgctgcct ttaggtataa taatgaatgt
     9541 tgaattatgc ggtaacacgc gtaagaactt aagttatcgg aggtgaaact aatgccaaac
     9601 attgaatctg caatcaaacg tgttcgtaca agcgaaaacg ctaatgtgaa aaattcatct
     9661 caaacaagcg ctatgcgtac tgcaatcaaa aaatttgaag atgctgtagc ttcaggtgct
     9721 gacaacgtag atgcgttata taaagaagct gttaaagcta ttgatatggc tgaatcaaaa
     9781 ggtctaatcc acaaaaacaa agctaaccgc gacaaatctc gtttaagcaa aaaaatcgct
     9841 aaataaaaga atcggcttcg gccgattttt ttatttgtca aaaaaaggac aaaaacaaac
     9901 gaccttcccg catgctttgc cctcgtcttt cttcttatcg atttcttaac aactgccctg
     9961 tgcgcaaaac gaatagttga aaacttaatt ctttttcgat ttgtcccgtt ttgatttgat
    10021 aatccatctc aactaattga tcaaacaact gtaccagcaa atgttcatcg aattttttta
    10081 cttcctgcat ggcaagtttt acacgatatg gatggatttt caatgtttca gcaatatttg
    10141 cctgctgata accaatcttt acaagaaatt ttgtctgtaa aaataaacgt aattgagaaa
    10201 gcaaaatcgc attaattttg attgtttctt ccccttgcat aactaaatct tcgtataaac
    10261 gcaaagcttg ttccgtcttc ccttttaaaa tatattgggt catgtcaaaa atattatgtt
    10321 ctaacgtttt aggaactaat tcttctactt ctcttaccgt gatttgctgt tgattttgtc
    10381 cataaaggac caatttctgt aactctctca tcattttgga taaatctaag tcggtcaacc
    10441 tcaagaataa atcaattgca cttcgttcca ttttgattga ggaattctct aaggtattca
    10501 tcagatattg acgtacttct ttttcattca actgttggac attgatgata attgctgttt
    10561 ttttcaattg tttcgttact tttttccgtt catccaactt ttcatatggc gcaaagaata
    10621 ctaatacagt cgattctaat ggttctttca aataagccgt caataaatca gtattttgtt
    10681 cgattccatt gtttactttt tcacctgtta aaaaataagg attctctacg aatactaacc
    10741 gttgatctcc gaaaaacggc aacgtctctg cttcagctac tacctcatct aaacttgcat
    10801 tttccatgtc aaaacttaaa aaatttaagt catcttcgcc ctctaattgg atttttttga
    10861 caatttctgt ccgaacttga tcttggagaa acttttctgt tcctaaaatc aagtaacaag
    10921 atgcaagttt ttctttacgt attttttgta actctgcttg tatactcaaa ttactcacca
    10981 ctttcaaaaa ttatcctacc atgagcagat gagaattact agtctatcat tgtttccact
    11041 agcccatgag gatgaaaaac tggatgccag cgataggtga tcattccttg ttgatccgtt
    11101 cgccatgttt ttatctgatt ttcttctagt gtctccaata cttcgctgtg cgggtgacca
    11161 taacgattat ttacgccgca ggatataatc ccatgttttg cttcaatatg ggaaagaaaa
    11221 tctgtgttac ttgacgtacg gctaccgtga tgacctaatt tgactacatc ggctttcaga
    11281 ttaggataaa tcaaaagcaa ttgtttttcc attgcttgat tcaaatctcc taaaaataaa
    11341 aagcgcctat tgttatattc catccataga ccaatcgaat cttcatttct tccctctccc
    11401 ttttcaaaag gagtcaatac ataaagatca taataaccgc tgatcctgtc cccatgtttg
    11461 atttcaatga tctcagtacc atttttttga gcttcagata aatgcctttg taatggctca
    11521 tttcgtccag cgccccaacc gatataaatt tttttgatag taaaatgctg agataacgtg
    11581 ggtaattctc ccacatgatc cgtatcgtcg tgtgtcaaga tgagtttatc gattacactt
    11641 atcccctgac cttttagata tggaacgata tttctttcag caggaggacg tatcatacct
    11701 tgcgcccact tttctctttc aaagtgtaac tgacctccag tatcgatcaa aatcgtttct
    11761 tttcgaaaag gactttgcaa aaagatactg tctccttgac cgacatcgat aaaagcgatc
    11821 gttgaagaaa agcgtaaaaa tggcaaagtc aaaaaaataa aagaaaaaag accaacataa
    11881 aacaagtgtt tcacaggttg ttctttcaac ctatccatac aaatcagtaa tccgaaaata
    11941 tacatcccag atagcacaag gggcaaacgt cctataatga gagacgaccc tgaagaaaaa
    12001 tcgattattc gctcaaataa gattaatccc tcttctatca gcaaaagtaa agtttccggc
    12061 agccagcgat tgaagagtgt gagaaataac acaccaggaa gaaaaagaaa cttgaatacc
    12121 ggtgcaatca gtaatgtaaa aagactacct aaaataggcc actcataaaa atgccataca
    12181 atcaacggca tcgtgatcat agtaaacagg acgtgagagg ccgttttttt caaccaatct
    12241 ccatcaaaag aaatcaggat aaacaaaaaa ctcatgaaaa aactaagctg tccacccgca
    12301 tgaaaaagaa atcgaggatc aaatagtaaa agaagccaaa agacaactgc aaataagtcc
    12361 atggtggaat aatgcttatt cagcaatttg actagcaatc gcaataaaaa cagcaaacta
    12421 gcacgcaaaa cagaaataga acttcctgca atagaaacat aacagatagt tagtagactc
    12481 aacggaataa tactgacgtg tagtggccat ccgatacgtc gaaacaaata atacagccac
    12541 cctaaataaa attggatgtg tagacccgat agactgaaca gatgcagcaa accactctcc
    12601 ctaaaaattt cttctggttc tctgaatcct gcatctttat agccccataa taaagaatta
    12661 atatatacac ttgttttttg cggaaattca gattggacaa aggagataaa agatcctctt
    12721 tttctctgaa gccagtttcg ccagtgattt tgcatggtga tctgttcaat cttttgaatt
    12781 tggaaccgtc cagaaattct atggttcctg aagtaagctt cttggtcaaa attataaaga
    12841 ttccgctttt ttgctggaga gatgaactcg ccttcagcag tcaattctat tccctttcct
    12901 cggaagttaa gccatgtttt caattctgct tctgtgttta cttgataaga aagctggagc
    12961 ttttgtttat tctctgataa ccttccttcc attgtcaaaa aattaccatt tgcacgaatc
    13021 gtatctgtaa atacagtaat ttgcgcagat gttgtttttt cctctaaaaa tgaatactct
    13081 tgtagcctat aagaaatgga taccgttatg ctacacaaaa tacacaagta cgcgataaaa
    13141 cgtccttctt ttagcgaaaa ccataaaaaa attccggcaa gaagcaaacg ataaccccaa
    13201 ttgtcagtgt aatacaacaa acagataatc atcgacgtaa ttgccggaaa aatcaaccga
    13261 tttcgaacat gcctttctaa aacgctagac atgttttcct gttaattcat tttgaagttt
    13321 tgaaaaatgc tgtgagtcta aagcaacttg gtgtacagat acacctaatt gttcgatcaa
    13381 cgcttgggca taaggatcat ttcgatagtc atggagataa ttgatccctt tcacaccagc
    13441 ttgcaacaaa gcctttgtac aggccaaaca tggaaaatgt gtgacataga tttctgaacc
    13501 ttccgtcggg ataccaagtt ttgcacactg taggagtgca ttcatctctg catggatcgt
    13561 tcgcaagcag tgtccatcca cgacgtaaca cccttcatct atgcagtgga catctccact
    13621 gactgctcca ttatagccgc cagcaatgat ccttttttct cgaacgagtg tcgctcctac
    13681 ttctaaacgt gtacacgtac tacgtaaaga taacagtact gcctgagcca taaagtactg
    13741 gtcccaagga atgcgttcat tatgtgactt atcagtaact ttttctttat tcgtatcgca
    13801 tgcttttgaa gatttttcta acatgtttta tattcctccg ttttttcaag ttgattaaaa
    13861 agtactcatt gcttaaacaa aatattcttt aaatataacg aatcatcctg tctgacaaaa
    13921 gatcctaccc ttcactgccg aaatgaagtc cgtcatccat tagcccatta taaaaattct
    13981 tcgaatccat ttgttcaaga aattccgtat aaaaatcgat acttcacaat catggcgttt
    14041 tgctacttct gtgagaacag agatatattc tttgatcaac ttgttattcc gatatttttg
    14101 ttttgtctca tcgactgctg gtggcgtgat aaagaaagca tgatcagaac tatagtcttt
    14161 tttcagctga actatcatcc actgcagatt ttttggtacg tttctttaga aagttgttta
    14221 tgctctgcta gatcattcgt cccaatgaga ataaatactt tatctgctgt atagctaagt
    14281 acatctttgg caaagcgttc tcgtagagct tgactgttat tgccgctgac agccgtattc
    14341 ataatatgaa tcccagatat tttattttgg agatagacat tcagcattgg tttttttaat
    14401 gtttctttgc gaacaatcaa gctatttcct atccaaaaat actacctcct cgtcctgcca
    14461 agctcttaga attggattat tcttagtatc cattagcgtc catttattgc tcgtgccagt
    14521 cgattttgaa tcattctatt taattgtaac gaagtttttt agtttttcaa ctgtcttttc
    14581 accgatacca gaaatctcct gcaggtcttc cactttctgg aaggatccgt tttcttctct
    14641 aaatcgaatg atttcttgcg ctttgactga tccgatccca ggaatctgct gaagttctga
    14701 tggagtggct gtattgatat ttattttggg aggctcggaa tctgctgtag gctgttttcc
    14761 ttccgaggat gccaactgat caagatgttc tggcagctct tcacctacag cagggatata
    14821 taggacttgc tgatcttcta tcttcgcaga aaagttgatt cgatcgctat ccgcttcttc
    14881 taataatcca cctgcacgct ttagtacatc atagacccgt tcttctgaag aaaaggaata
    14941 gataccaggc tccttcacac ttcctttgat atcggtgtac atgacctttg gacgagcatc
    15001 ctcttctaca tcgtttcctt cagctgatgg atcttctcca ctggaactaa acgaagaaag
    15061 tatttctttt gcagcagtct cttctgtttc tctataaaat agcaaatttg ataataaaaa
    15121 accacttgcg atacaaaaga taagaactat catagacatc caaatgatca atttgggtga
    15181 agctagtatt ttcttgctga ttttttctat cattgttctt ttttcaaaga atgaccagta
    15241 aaaatcattc attactcctt tcatttaaaa ttgaagaaac agtgagctct ggcaaaatcg
//
LOCUS       DBKOIJ010000059        15253 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000059 DBKOIJ010000000
VERSION     DBKOIJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.061"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1019
                     /locus_tag="ACV5NJ_001441"
     CDS             <1..1019
                     /locus_tag="ACV5NJ_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HHY6485539.1"
                     /translation="SIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLITG
                     KLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFFT
                     SIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMII
                     SILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVF
                     LSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILL
                     FVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVR
                     RLKR"
     gene            1016..1783
                     /locus_tag="ACV5NJ_001442"
     CDS             1016..1783
                     /locus_tag="ACV5NJ_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HHY6485540.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1883..3451
                     /gene="liaX"
                     /locus_tag="ACV5NJ_001443"
     CDS             1883..3451
                     /gene="liaX"
                     /locus_tag="ACV5NJ_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HHY6485541.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3748..4068
                     /locus_tag="ACV5NJ_001444"
     CDS             3748..4068
                     /locus_tag="ACV5NJ_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HHY6485542.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4068..4418
                     /locus_tag="ACV5NJ_001445"
     CDS             4068..4418
                     /locus_tag="ACV5NJ_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HHY6485543.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4585..5523
                     /gene="hprK"
                     /locus_tag="ACV5NJ_001446"
     CDS             4585..5523
                     /gene="hprK"
                     /locus_tag="ACV5NJ_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HHY6485544.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5538..6368
                     /gene="lgt"
                     /locus_tag="ACV5NJ_001447"
     CDS             5538..6368
                     /gene="lgt"
                     /locus_tag="ACV5NJ_001447"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HHY6485545.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6412..7437
                     /locus_tag="ACV5NJ_001448"
     CDS             6412..7437
                     /locus_tag="ACV5NJ_001448"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HHY6485546.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7452..8390
                     /gene="galU"
                     /locus_tag="ACV5NJ_001449"
     CDS             7452..8390
                     /gene="galU"
                     /locus_tag="ACV5NJ_001449"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HHY6485547.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8515..8955
                     /locus_tag="ACV5NJ_001450"
     CDS             8515..8955
                     /locus_tag="ACV5NJ_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HHY6485548.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8958..9560
                     /locus_tag="ACV5NJ_001451"
     CDS             8958..9560
                     /locus_tag="ACV5NJ_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HHY6485549.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9623..10726)
                     /locus_tag="ACV5NJ_001452"
     CDS             complement(9623..10726)
                     /locus_tag="ACV5NJ_001452"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HHY6485550.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10936..11937
                     /gene="ccpA"
                     /locus_tag="ACV5NJ_001453"
     CDS             10936..11937
                     /gene="ccpA"
                     /locus_tag="ACV5NJ_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HHY6485551.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12292..12687
                     /locus_tag="ACV5NJ_001454"
     CDS             12292..12687
                     /locus_tag="ACV5NJ_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485552.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12687..12938
                     /locus_tag="ACV5NJ_001455"
     CDS             12687..12938
                     /locus_tag="ACV5NJ_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485553.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12981..13376
                     /locus_tag="ACV5NJ_001456"
     CDS             12981..13376
                     /locus_tag="ACV5NJ_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485554.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13373..14629
                     /locus_tag="ACV5NJ_001457"
     CDS             13373..14629
                     /locus_tag="ACV5NJ_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HHY6485555.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14641..14964
                     /locus_tag="ACV5NJ_001458"
     CDS             14641..14964
                     /locus_tag="ACV5NJ_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485556.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14957..15223
                     /locus_tag="ACV5NJ_001459"
     CDS             14957..15223
                     /locus_tag="ACV5NJ_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485557.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5240 a         2580 c         3225 g         4208 t
ORIGIN      
        1 ttagcatcat cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag
       61 ccgctttata tgaagtacct gtcatcgaga tagaaaaagt acgaataaaa gaggatcatc
      121 aattgattac aggaaaactg atgaactcgg atcaaaaagg agaatcgatt tatttatcgg
      181 ttccttatca aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag
      241 tccaaatgca tggagatgaa tggcagctag tagcaaaaaa aaatgatggc tggcttttct
      301 tcttcacaag tatcttcatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg
      361 ctttaggaag tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta
      421 aaacagaagg gatttctcta ttaaataaat ctatcctgtt cgtcgttata gggatgatta
      481 ttagcatatt ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag
      541 ttacgagtgt ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc
      601 aaggactgcg ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt
      661 ctagcgtact gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga
      721 cattagaaga attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag
      781 gaaggcaagt gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata
      841 tcagtgggtc agttccatcg cttcttttat atttaggaaa tcaatggtct ttcaaagaaa
      901 cgatagaaca gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag
      961 tattatcgat ccccatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa
     1021 tgtacttatt atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca
     1081 aggtatgtat attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt
     1141 cggttatcat cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc
     1201 tgtggttatc ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag
     1261 tatactgatc tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat
     1321 ccaaggtttt acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat
     1381 tgattttaga ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga
     1441 tgcgagtgta gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc
     1501 tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt
     1561 aatgacactt ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga
     1621 tttagacatt ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat
     1681 ggctattctt accacattgt ccgcactaat tgttcttcct ttaacctgcg tgactacagc
     1741 atatctcttt acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc
     1801 ctatgacatt tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat
     1861 tttgcttaag gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa
     1921 aggaatcctt tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga
     1981 cgaaaagcaa atcaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagataa
     2041 ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga
     2101 tccaaaggaa agagaaagac aagatcaaga aaacttagaa aagatcttag atgaactagc
     2161 aactgaagcg aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa
     2221 taaggcagca cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt
     2281 aggcgagttg acagaagaag aattagcaca gcgtcaggcg ttagaggcag aaatcaaaga
     2341 gttagaagct agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa
     2401 agatatccgt aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt
     2461 gccagatgat tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc
     2521 agaagctgga tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac
     2581 agtgaatgac aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac
     2641 gaaatttgag catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc
     2701 agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa
     2761 gatcaaactt tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca
     2821 gattgaggtt aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga
     2881 ccttgttttc tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg
     2941 caacatcatg attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa
     3001 catcatcgtg gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat
     3061 cgatattcga aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac
     3121 atttggtgcg acagcagaaa atctttctgt gtctttagta aatggtgatg tacgtcttac
     3181 gattaaagaa gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt
     3241 cgctttacca gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa
     3301 tagccgtctg tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt
     3361 gcaattccgt cgtgtttcag atgatgagat cgcgcaagtg caactttcaa cgacaacagg
     3421 aagtatctat ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg
     3481 agcaataaga agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg
     3541 ccgatagtct atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc
     3601 gagtcacaag gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg
     3661 accgagaatt ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca
     3721 tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atctaataaa
     3781 aatgttgttt tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact
     3841 gttatccgtg tgctttatgt attcctgagc ttagttgctt ttggaagtcc aattatttta
     3901 tacattttat tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat
     3961 aacaatgcat attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa
     4021 cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc
     4081 aacgtatttt agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg
     4141 tccatgtgag cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata
     4201 tgtttgtcaa accgattttg acgatactgt ccctgccctt tacattagtc acattgggat
     4261 tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag
     4321 aacattttag tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg
     4381 tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc
     4441 ggcgcagttt ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa
     4501 aaacgttttt tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac
     4561 tctaatagaa atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa
     4621 aaattatctt tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact
     4681 ggggacatct cacgcccagg actagagttg actggttatt ttaattatta ttcccataat
     4741 cgtttacagc tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag
     4801 cgtttgatca tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga
     4861 ggattaccag ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg
     4921 cgttctccca tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc
     4981 cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg
     5041 attcaaggtg acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggc
     5101 catcgattga tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc
     5161 ggagaaccgc ctaaaatcct ggaacatctg attgaaatcc gaggaatcgg gatcattgat
     5221 gtaatgaatc tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt
     5281 tatctggaag catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc
     5341 gttgaaatcg cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac
     5401 gttgcaatca tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac
     5461 gcaacgaaaa catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac
     5521 tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga
     5581 ttcctatcta ctggtatgca ttgatcatcg tatcaggtat cattatcgct atgtggctta
     5641 gcagcagaga ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt
     5701 ggggattgcc tatctccatt attggcgctc gcttatatta tgttttattt gatcttcccc
     5761 agtatatagc tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg
     5821 gcggactgat tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt
     5881 tgtggacttt tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt
     5941 ggggaaattt catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa
     6001 gtctgcattt accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac
     6061 caacatttct ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac
     6121 gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata
     6181 gtttcgggcg tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga
     6241 tccgtatttc tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt
     6301 ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa
     6361 ttatctgatt tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat
     6421 agagtagcgg ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa
     6481 aacggacatg acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc
     6541 caacacacga atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc
     6601 accaatatgg aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa
     6661 gcaatacgtt ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc
     6721 catgcaagta aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag
     6781 gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa
     6841 gaagtagcaa aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca
     6901 aaatatgtac aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg
     6961 atcggtgtag agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt
     7021 cacggcttag ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag
     7081 atcagccgtt taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga
     7141 ataggcgatc tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac
     7201 ttgttaggac aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa
     7261 ggtgtgaata ctacccaagc agcgatggaa ctagcccaaa tgatggaaat cgaaatgccc
     7321 atcactcaaa caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa
     7381 gaaatcatgt tacgtgatgg aaaaaaagaa aatgaattct cattaacaat tttttgaagg
     7441 agcgaatgta tatgaaagtt aaaaaagcag ttattccagc agcaggatta ggaacaagat
     7501 ttttaccagc aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa
     7561 tccagttcat cgttgaagaa gcgttgaaat caggtatcga agacatcttg atcgttacag
     7621 gaaaagctaa acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc
     7681 taagtgaaaa aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact
     7741 tgcatttcat ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag
     7801 cctttattgg aaatgaacca tttgtcgtta tgctaggcga tgatataatg gaagacgaag
     7861 taccattgac acagcaattg atcgatgact atgagcgtac acacgcttct acgattgccg
     7921 ttatgcaagt tcctcacgat gaaacttcaa aatacggaat catcaaccca ggggaagtac
     7981 tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac
     8041 caagtgattt agctattatt gggcgttact tattgactcc agaaatcttt gatgtattat
     8101 cagaacaaca accaggtgct ggaaatgaga tccaactaac tgacgcaatc gatacgttga
     8161 ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa acgttatgac gttggtgaca
     8221 aatttggttt tatgaaaaca agtattgaat atggtctgac acatccagaa gtaggggcac
     8281 ctttgcgtga atacatcatg tctttaggta aaacattaga agcagaagga agcaagatca
     8341 atcataatcc aaaaggaata acagaaatca aagaagaaaa gaaaaaataa tctttttttc
     8401 taacgagctt cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa
     8461 aaaacacgaa aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca
     8521 ggaggagaaa ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgttttcatc
     8581 gttcgcgtat tgttgcaagt ttccaaaact ttagaaaaag tagaccagac tgtcgcagaa
     8641 gcaaatacga caattgagat cgtcacacaa gacgtagatc ttttatctag acaagtagaa
     8701 ggcttgctag tcaaaagtaa tgagctgctc ttggatatca atggaaaagt cgcaacgatc
     8761 gatccattgt tcacagcagt tgctgattta agtgaaagtg tatcggaact taatcattct
     8821 agtaaaaaca tcgtgacgaa agtcggcgga atcggaaaaa cgacagctaa agcgactgtt
     8881 gccggaaaag tcggtggaac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg
     8941 acaggaggaa aataagaatg gctaaaaaag gtggattttt attaggtgca gtagttggag
     9001 gtacagccgc agcaatcgcc gcattattgt tagcacccaa atctggtaaa gaacttcggg
     9061 atgaattggc tgctcaagca gatgatttga tggacatggc ttcagattac acagatatgg
     9121 caaaagaaaa atcatctgag ttaacaggta ttgcaaaaga aaaagcgcaa ggtctttctc
     9181 aacaagcagg ggatctagca ggcaatgtta agcagaaggt aagtgaatca gcagaggaag
     9241 caggaagtga atcagatgat atcttgttaa acctcaaaga acaatcagct gatttatcta
     9301 atcgtttcaa aaaaacagca gaagatctga aaaagccagc agacgaatta ggtagtattg
     9361 ctaaagatac ttcagaagat atcatgatcg atgtaaaaga tgctgcagat aaagcaggag
     9421 agacggtcaa tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatgt
     9481 tgaaaatggc aaaagaagat gcaaaagaag ctaaaaaaga aatgaaagat acaatgacag
     9541 attcgactac caatcactaa gattactcat ttctagaata aaaaaggtga gagcggacca
     9601 gctctcacct tttttatttt cattactcta aaacaagtaa ttcttttggt gtagtcgtga
     9661 atggcacaca accatcactt gtcacataca cacaatcttc gatacgtacc cctacttttt
     9721 caggaatata gattcctggt tcaagagaga agcacatccc ttcttcaatc actaagtcgt
     9781 ttccttctac aagagaaggg aattcgtgga cggttgttcc gattccgtga cctaaacggt
     9841 gattgaaata ttcgccgtag ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt
     9901 cacctgctgt cacacctggg cgaactgcct cttgtgcggc aagctgtgct tcaagagtga
     9961 tgttatagat tttttcttgg aactcagttg gtttttgata agcaactgta cgagtagcat
    10021 cactgcaata gccattccaa ataacgccaa gatcgaataa cactagttca tttggtgaaa
    10081 ccttcgtgct tcctggtgta ccatgagggc ttgcagcatt tgctcctgca aggactaatg
    10141 tgtcgaaaga catttgtgaa acaccttgtt tttttaattg gtattcgatt tctgcgataa
    10201 ttgcttgttc agttacgcct tcttttactg ttttgaagcc aatttcaaag gcaacatcgg
    10261 cccatttacc agcttcgatc agtttgtctt tttcttctac cgttttgatc agttgcagac
    10321 gctggataat agaagttaga tcggtgtaag aatctgtacc tgtcaagata tcagagatag
    10381 catcgaagcg atcgacagtc agagcattct tttcaatacc tacattacca ggttgcccat
    10441 agcgtgcgcg aagctctttt ccgatgatct cccaaggatt ttcgctgtct aagtagccac
    10501 gtacatcgta cgcccagctg ctgttttgag catcttcaac ttccagagca ggagtgaaaa
    10561 ggaaaggatc ttgttccaga gggataaaaa gagctagtac acgctcgtga ggatcacttt
    10621 catatccgga aaagtaagcg atgtggccag ggtcgctgat ataagccaat tctacttggt
    10681 gttgtgccat ccattgacgt agttcattga tttttacgtt attcatttgg caacctactt
    10741 tctaaattaa ttttgtattc aataattgta ccactgattg attaaaagac caacgttctt
    10801 aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata aaacggttgc aattgttgag
    10861 attatttgat atcctatttt agaaatgtaa gacgtatttt cagaaaacat ataaaatatg
    10921 aataggagaa cacaaatgga aaagcaaaca atcactatct atgatgtagc gagagaggca
    10981 aatgtttcga tggctactgt ttcacgtgtc gtaaatggca atccaaacgt caaaccagca
    11041 acaagaaaaa aagtcttgga agttatcgat cgcttggatt atcgtccaaa tgcagtcgca
    11101 cgtggcttgg caagtaagaa aacaacaaca gttggggtaa tcattcctga tgtcagcaat
    11161 atgttctttg cctctttagc acgtggtatc gacgatgtgg caacaatgta caaatacaat
    11221 atcatcttag caaactcaga tggcaacgat caaaaagaag tcaatgtatt gaataattta
    11281 ttggctaagc aagtggacgg cgtgatcttt atgggtcacc atatcacaga cgaaatccgt
    11341 ggagaatttt cacgttcaaa aacgcctgtt gtactagctg gttccattga tccagatgaa
    11401 caagtaggta gcgtaaacat tgattatgtg tcagcaacaa aagatgcagt aaataaatta
    11461 gcaaaaagcg gcaataaaaa aatcgctttt gtcagcggtg cgctgattga tcctatcaat
    11521 ggtcaaaacc gtctaaaagg ctataaagaa gcattaagcg aaaacaatct accatatagc
    11581 gaaggattga tttttgaggc acaatacaac tttaaagatg gtctgtctct agttgaccgg
    11641 atccataata gcggtgcaac agctgtctat gtttcagacg atgaattagc cattggtatc
    11701 ttggatggtt tattagaccg cggcgtaaaa gtaccagaag aattcgaaat catcacaagc
    11761 aacaattctt tactgacaga agtagctcgt cctcgtttga caagtatcac acaaccatta
    11821 tacgatttag gagcggtatc catgcgccta ttgacgaaaa tgatgaataa agaagaagtc
    11881 gaagaaaaaa cgattatctt gccttatgga atcgaagaaa aaggatcaac taaataataa
    11941 aagatcaatc atttaaaggt tgttccaaaa gtggtcaact tctggaacaa cgtttatacg
    12001 agaaaggggg ttatgacaaa attttgtcat aacccctttt tttttgtgac tttaaaaaaa
    12061 gagaaaatgg cggaaacaaa ccttacgtcc atcgtatacg acacaatctg tgcgtagatg
    12121 ctacattttg ttcactacta tcaaaattac tttaaaatga aaacgtgata aaaaagaaag
    12181 tagaaaagta gataagtaac tctaaaaaat ccaaaaaaat ccaggctcga ttgcgtactt
    12241 atcttaaagg agaaacaatc catcatatat aacccgaaag gagacttcta tttgcctaag
    12301 aaaagacgaa agaccatagt catcattttg accattctta ctataggaat cggagggaaa
    12361 ttttacatgg acaaaagaga aatgcaaaga cagaaagatt tactcgcagt agaaaaacag
    12421 tccgtcaaag ttctgaaaaa tacctttgcg gatatcaagg aagtgaagat tgaaaaatct
    12481 gctcgaaatg aaatgacggg ttcataccgg atagttattc taatgactaa taaacaagat
    12541 caatcgattt atttttctta tagtttttgg aaagaaagaa atgagatagg aagttacgga
    12601 atagttgatg aaaaaaagca aaaagaagga aatacgctca ataaagttaa agtaacttac
    12661 tctaatggaa atgaggaaag tatttaatga ttactgacaa acaatatagt gaattaagtg
    12721 atgctgttta ctggctagat cctaaacata gagactatgt acctgaaatg caagaaaatt
    12781 taagttttaa aattaacggc actatgtata aaatcctaaa aatcaaaaac tccttcgacg
    12841 gcatgcaggc gatggctgta gcgccaatcg tccatagcaa attggagaaa aatttcaaaa
    12901 ataaaaaaat tcctgcaaac tttcgcgtac ttaaataatg aaggtgtcaa tacttacata
    12961 aaaagaaagg aggcacgttc ttgtcaagga aaataagaaa actcagctta gccattttat
    13021 taatcgtcat attagggatc ggagggaaag tctatatgga caaaagagaa gcacaaaggc
    13081 agaaagattt actcgcagta gaaaaacagt cagtcaaagt tctgaaaaat acctttgcgg
    13141 atatcaggga agtgaaggta gaagaattta aaaaaaaccc tgttaccgga agttatggtg
    13201 cacttgtgac aatgacaaat aatgaaggga aatctgttta ttttgattat agtttttgga
    13261 aggagagaaa tgaacttggt ggttttggca ttgagaacag agcggtacaa aagacaggag
    13321 tcactataaa aagagtaaaa gtaatttttt ctaacggaca ggaggagtta gtatgacaat
    13381 aaacgataag cattacaatg atattagtga gaaggtttat tggctagatc ccaaatatcc
    13441 ccgctacaat gagggataca aaaagaactc tgttaaagaa tttgctggta tggaatttca
    13501 aatcctacaa atcaaagact cattggacgg catgcaggcg atgtctgtag cgccaatcaa
    13561 gaacggtaaa gtggatcttt ctcaagtagt gattgctttt gcaggaacga acttaagtga
    13621 ttggaaagat ttggaaacgg atgcaagaag tatcatgaat ctctacggga atagaccagg
    13681 agcaagtttt tctgaaaaag caacaacaaa caagatttta tccgttagtg gattgaaaac
    13741 agcggggcaa atcaattcag cgattgcatt tgctaatgaa gtaaaaaagg aatatggaga
    13801 agctgctatt tctactaccg gccattcatt aggtggattc ttagcattat atgtagcagc
    13861 agagaaacaa tggaaaaatg tcggcttcaa cgggccggac ccgtatgatc tcttatcacc
    13921 agaagcaaaa aaatgggtgg aagaaaatcc cggtatgctc accaattacc gaaatcacgc
    13981 cgattttatc ggtaattttg gtggtaatgg cacaggtgct gaagttcgga tcagtatgga
    14041 aacagcattc ttgaatctgc ttgcttccca cagtttaacc tcttggaagt ttgatgaaaa
    14101 tggaaagctg attattcctg aaaatgaaaa caatgttaaa gctcgaaaac aacagaaaga
    14161 aaaccaagcg acccagttgt tttcctatca gttgcaagaa ctgaacaaca tacgtaaaaa
    14221 actacgatta agtggcggcc ggctgacagt taatgaacaa atttacttag atgacacaca
    14281 agctaggtta gtagtggaaa agttatcggc aaaaatgaaa agcgctatgg aagaagttat
    14341 ccgcatcaat caaagaggaa tttttgagct agagaaaaaa tggcaagaag gcttgcggca
    14401 agcacgtgtt gtagcccctt cgttgactga acaggaaatc atagaagcat tagctagtgt
    14461 gggcgccaca aaagaaaact tagtcgattc tgcgaaagaa gtctatctgc tacgaatcaa
    14521 taaggcaaga cgtatggggg aggcctttga tacattggcg aaggagatcc aagacaaaat
    14581 caatgaaatc gtggcacgag atcgagaatt agcacagcaa ttcaattaaa ggagaagtag
    14641 atgaaactag aagaattgga aagagattat cgaagtgaac gaagacaatt agaagctcaa
    14701 gaagatgaat tgatccactt taagaatcaa ggattacgat tgatggagca agaggagcag
    14761 cgactgcatc atgtattaaa gaacttagct ttagataatg agcctctgtt tcaagcaaga
    14821 cgaagttatt cgaattatca ggagcattat caacaagccc tacaagtaga acgacgaaaa
    14881 ctctatcagc agcaagatga actagaacgt tcctatcgtc aaaaaatcag acaaataact
    14941 atggagaaat catcttatga atgaattttt aatgcaatta ctaaaagaat tagagaaaaa
    15001 tctacatcga tcaaatgtac gttcagttgg tatggggata tctcagcaaa tttgggcaga
    15061 aaaacgattg acagatcaaa actaccaaat gactgcagaa gaagctgctc aagaattttc
    15121 taaccgacat gtacacttga aaggatcagt aaaagggcaa tttgcagaaa aactaactga
    15181 attattccaa agacaagaaa agaaattaaa acagtttcgt tagtaaagtt atatagaaaa
    15241 attttgtgaa aat
//
LOCUS       DBKOIJ010000060        14625 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000060 DBKOIJ010000000
VERSION     DBKOIJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.062"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            4..816
                     /locus_tag="ACV5NJ_001460"
     CDS             4..816
                     /locus_tag="ACV5NJ_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HHY6485558.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACV5NJ_001461"
     CDS             813..1613
                     /locus_tag="ACV5NJ_001461"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HHY6485559.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACV5NJ_001462"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACV5NJ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HHY6485560.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACV5NJ_001463"
     CDS             3110..4819
                     /locus_tag="ACV5NJ_001463"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HHY6485561.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACV5NJ_001464"
     CDS             5031..9389
                     /locus_tag="ACV5NJ_001464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HHY6485562.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACV5NJ_001465"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACV5NJ_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HHY6485563.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACV5NJ_001466"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACV5NJ_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HHY6485564.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /gene="rnpM"
                     /locus_tag="ACV5NJ_001467"
                     /gene_synonym="ylxR"
     CDS             11227..11520
                     /gene="rnpM"
                     /locus_tag="ACV5NJ_001467"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HHY6485565.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACV5NJ_001468"
     CDS             11517..11828
                     /locus_tag="ACV5NJ_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HHY6485566.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACV5NJ_001469"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACV5NJ_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HHY6485567.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACV5NJ_001470"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACV5NJ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HHY6485568.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DBKOIJ010000061        14431 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000061 DBKOIJ010000000
VERSION     DBKOIJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.063"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(85..294)
                     /locus_tag="ACV5NJ_001471"
     CDS             complement(85..294)
                     /locus_tag="ACV5NJ_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485569.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(305..1633)
                     /locus_tag="ACV5NJ_001472"
     CDS             complement(305..1633)
                     /locus_tag="ACV5NJ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485570.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1769..1924
                     /locus_tag="ACV5NJ_001473"
     CDS             1769..1924
                     /locus_tag="ACV5NJ_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HHY6485571.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(1998..2585)
                     /gene="yihA"
                     /locus_tag="ACV5NJ_001474"
     CDS             complement(1998..2585)
                     /gene="yihA"
                     /locus_tag="ACV5NJ_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HHY6485572.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(2668..3918)
                     /gene="clpX"
                     /locus_tag="ACV5NJ_001475"
     CDS             complement(2668..3918)
                     /gene="clpX"
                     /locus_tag="ACV5NJ_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HHY6485573.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(4165..5454)
                     /gene="tig"
                     /locus_tag="ACV5NJ_001476"
     CDS             complement(4165..5454)
                     /gene="tig"
                     /locus_tag="ACV5NJ_001476"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HHY6485574.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(5608..6540)
                     /locus_tag="ACV5NJ_001477"
     CDS             complement(5608..6540)
                     /locus_tag="ACV5NJ_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485575.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(6697..7356)
                     /locus_tag="ACV5NJ_001478"
     CDS             complement(6697..7356)
                     /locus_tag="ACV5NJ_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HHY6485576.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(7359..8384)
                     /gene="sppA"
                     /locus_tag="ACV5NJ_001479"
     CDS             complement(7359..8384)
                     /gene="sppA"
                     /locus_tag="ACV5NJ_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HHY6485577.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(8397..8687)
                     /locus_tag="ACV5NJ_001480"
     CDS             complement(8397..8687)
                     /locus_tag="ACV5NJ_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485578.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            9096..10901
                     /gene="glmS"
                     /locus_tag="ACV5NJ_001481"
     CDS             9096..10901
                     /gene="glmS"
                     /locus_tag="ACV5NJ_001481"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HHY6485579.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(10973..12328)
                     /gene="glmM"
                     /locus_tag="ACV5NJ_001482"
     CDS             complement(10973..12328)
                     /gene="glmM"
                     /locus_tag="ACV5NJ_001482"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HHY6485580.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12352..13521)
                     /locus_tag="ACV5NJ_001483"
     CDS             complement(12352..13521)
                     /locus_tag="ACV5NJ_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HHY6485581.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(13518..14405)
                     /gene="cdaA"
                     /locus_tag="ACV5NJ_001484"
     CDS             complement(13518..14405)
                     /gene="cdaA"
                     /locus_tag="ACV5NJ_001484"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HHY6485582.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
BASE COUNT         4061 a         3090 c         2529 g         4751 t
ORIGIN      
        1 taaaaattat aaatcagtca aaaaaaaata aaaaaccagt cagaaagact ggttttttat
       61 ttttttgtct atcatcaagc cgttttatcg ttttttattt tcgcttcttg tttttttcat
      121 ataaacagaa gcaggttttc tttccttatg aagaagataa taataaagtc ccatcccgcc
      181 aaacatgacc aaaaaaccga tgactgatcg aataggaaaa ttaggaacaa aaaagagaca
      241 gacggataat aatgatgccg gaaccattat tcgataatag agttgtttat ccattttctt
      301 cacctcatat tttttgtaaa gtaatcaatt gttctaattc tcggtaatct aaaaattgca
      361 cacgcagatg ctgctcacct ttcttgacat cgaattggat acttcgccgc tttttcttta
      421 ttcgtttgaa ggtaacatca gagagtctat gtttgatttc ctttaacggt aattctgaca
      481 gggattctga aggaacatag attcttaagg ataaaagata ctcttgatat ttttcatctg
      541 ttacctctac ttctagatat tccctaatat aataacaata agcttcgatt ccattatcca
      601 aagcaacagt cggatcgata actttatagc acatcatgcg tgatcgattc tcgtcaaccc
      661 aaggaaaata cgcctgagca tttagtgttc cgcgaacacg cctttcttta taatctcctt
      721 gttggatatc ataacttttt gttacaaaaa atgaagcatt ctttttagga acaggttgca
      781 tttgtgcatt ctgatactgg acagattgga tcgtatggtc agtgtaaatg tattcaacga
      841 atggcggttt caataaaccg acaccattgt cccaagcaag atgatcaaac ccaagctggt
      901 ccctaaaata ggattgaaac atttgattgt ttggcttttg ccagttaaag gggtctcgat
      961 aaagctggat acatatgact tcgttttcta attccttcac tttttctact tgttggatgt
     1021 cgagaaaaat cggtttaggg atgatttgat gataatagcg actataatac atttcccaac
     1081 acgaagtgaa acataaacct tggtaaaaaa gatcgtatcc tggaaattgg ttgcaatcga
     1141 ctactacttc cccgttttgt tgtttcattt taggtaaatg ttcgatttca tcctttttct
     1201 caaacaatag ccgttgagag atctgttttg tattgtgata gataaactcg ctataagctc
     1261 gcatataggc gaacacccca tgagcaatca tttttttctg aatgaagtct cgaatcaaaa
     1321 attgcttttc tttgaaaaca gagatagaca atacattttt tatcaataat tgattatctt
     1381 ccaagcgaaa actacattcg ttctcgtcat ttcctacaac taaccaactt tcgtctctta
     1441 ccgatgctag cattattttt tcttgaagct ttcgaagatc atacggatcc gcttgttgtg
     1501 tgaaagagcg gataaatgtt ggctggatca acagacgtgt caaaatagcc caatcgctta
     1561 tttcctgttt tttttcttct tcatttaagt ctgtgagctg gatagtcatc aataatttat
     1621 cttcgccaat cattttctca cctctttcaa atctttttat aatttaatta taaccaatga
     1681 taaagaaaaa ctaaaacttt atgtcttaga aaccaatttg gacgattaca tgatagacta
     1741 agtaaaaaga aacaaagagg tgtttacaat ggggttgaaa tttgaacgta cagataattt
     1801 agataaactt ttcgaatcat ttgctgtcga tccggaaaaa acagttgcac caaaagaaga
     1861 gatcgatcga tttctaaaac cacagccaca aaatgaaaag aaaaaagaca agaaacaaaa
     1921 gtaagttaag ctcaaaaaaa gacttaccag ccaaactttt atgaaagagt ttgactggta
     1981 agtctttttt attcttttta tgattcaatt gcttgttcga ttgctgccca cgcttcttct
     2041 tttccctgtt ttgtaacgga agaaaagaga ataaacgtgt cattgcgatc aaaatccaac
     2101 gcttttttga tttcgctctc atgcttgttc catttaccac gagggatctt atctgcctta
     2161 gtggcaacaa caattacagg gatatcataa tacttcaaaa attgatacat ctggatatct
     2221 tcttgagaag gcttatgacg caaatctact aaagaaacga ccgcacgtag ctggtctcta
     2281 gtcgttaaat atgtctcgat catctttccc cattttgctc gctcagtttt ggatactttt
     2341 gcatacccat atcccggaac atcgacaaaa tgtaatgcgt tctcgatcaa ataaaaattc
     2401 aatgtctggg tctttcctgg cttaccggaa gtacgggcta aattctttcg atcaatcaat
     2461 gtgttgataa aagaagattt ccctacattt gatcgtcctg ccagagctat ttctggcaat
     2521 tgtgtatctg gatattgttt tggtgccact gcacttatga taatttcagc ttgatggact
     2581 ttcatggtct ctctccttaa caaggtgatg aataaagcaa gcggctgtcg caaaagccaa
     2641 aatgactttt acggcagcct cttttcatca acccgctttt ttctctgaat ctttatagat
     2701 tactgttggc tcaccagaca attcagctgc ttcttttgtg ataatcactt tttcgatgtt
     2761 ttcatttgaa gggatatcaa acatcacatc catcatgatt tcttcaatga tggaacgaag
     2821 tcctcgagca ccggtattgc gttcgattgc ttttttcgca atggctctca acgcttcagg
     2881 ttcgaattca agttcggtat tatctagaga aagaagtttt tgatattgtt tgactagcgc
     2941 atttttcggc tcagtcaaga tccgtaccag atcatctgtt gtcaatttct ctaaagctgc
     3001 cataacaggt aatcgtccga taaactcagg gatcaatccg aattttagta aatcttcagg
     3061 aatgatatgc tgcatgacac tttcgtcttc agaaagtttt tggttttgtg taccaaatcc
     3121 aatcgtcttt tcgcccatac gattcttcac aatcgtttcg atgccatcaa atgcaccacc
     3181 tacgatgaac aatatgttcg tcgtatcaat ctgaataaat tcttgatgtg gatgtttacg
     3241 tccgccctga ggtggcacac ttgcaacagt tccttctaga attttcagca aggcttgttg
     3301 gacaccttcg ccggaaacgt cacgagtaat agatacattt tcgcttttac gcgcaatttt
     3361 atcgatctca tcaatataga tgatcccttt ttctgcaagt tccacattgt aatcagcaga
     3421 ttgaagcaat ttaagcaaaa tgttttctac atcttccccc acataaccag cttctgtcaa
     3481 acttgtcgca tctgcgattg caaaaggtac attcaatgtt tttgctaatg tttgtgctaa
     3541 aaatgtttta ccagaccctg taggtccgat caagcagata ttgctttttt gcagttcaac
     3601 atcatctgtg ttttcttgtg cttgctgatt gacacgttta tagtgattgt atacggcaac
     3661 agctaacgag cgttttgcgc gatcttgtcc aatcacgtag ttattcaata cttcaagaat
     3721 ttctagtggt ttagggactt cagtgaattc gcgtacagct tcttcataaa attcttcatc
     3781 aatgatttct ttacataggt cgatacattc attacagatg tagacccctg gaccagctac
     3841 gatctttttc acttcttctt gggttttccc gcaaaaagaa cagcgtacgg tctcattgct
     3901 acttggattg tcatacatgg tcttcacccc ttaacttgca aaaagtatgc aagaaactat
     3961 caaagacttt acagtctttt actatcataa catagtttag taaaaagaaa aagtgtttaa
     4021 caaaggatca actgacctat acctttgcat tttttactct taacaaaaaa taaggaactg
     4081 tgacaaaatc gtgtcacagt cccttatttc gaataaacaa ttttctagaa gtagcttctt
     4141 cagaaatgat ttattacttg atttctattc tttcgcttct gtcgctgtac ctgtgatcaa
     4201 ttcgattgct tttttcattg tcacatcatg ttccagcata tcttctgtca acacgcgttt
     4261 gatttgttcg attggcatgt tgtatgtttc tgataattct tggatttctg cattgatttc
     4321 ttcttctgtt gcttgaagat tttcagcagc tgcaattgct tcgatcacaa gatttgttct
     4381 tgtgcgcatt tctgcttcac cttcgaattg tttatgcaag tcttcttctg tagaaccagt
     4441 taattgatag tacatttcag gagagatacc ttgacgttgc atgttgttca agaattcgtc
     4501 cattgaacga tgtacttcat catgaaccat cacgtgtggc aattcaacga tttctgcgtt
     4561 ttcaacagcc atacggatcg ctgcttcatc ttttgcatcg tccgctgctt tttcttttgt
     4621 ttctactaat tctttgcgat atttttcttt caattcgtct aatgttgata cttcgtcatc
     4681 aacatctttt gcgaattcgt catctaattc aggcacttct tttgctttta cttcatggat
     4741 tgtcactttg aagactgctt cttttccagc aagatcttct gcttgataat cttctgggaa
     4801 agttacattt acatctagac tttcacctga tttatgacca actaattgat cttcaaaacc
     4861 tggaatgaat gagcctgaac ctaattcaag tgagtaattt tcgccttttc cgccatcaaa
     4921 cgcttcgcca tctacaaagc cttcgaagtc gattactact gtatcgccat tttcagctgc
     4981 agcatcttct ttgataacta attcagcttg tgcttcttgt tcacgtttga tacgtgcatc
     5041 cacgtcagca tctgttactt cacggtcttg tttttcaaca actagatctt tatattgtcc
     5101 taattttact tctggtttca ctgttacttc agcagtgatt acccagtctt gacctttttc
     5161 catgctttca acatcgattt ttggttgaga aacaggatcg atgccagctt ctttgacagc
     5221 agcttcataa gcttctggta atacagcatt caatgcatct tcatataaag cttcttcacc
     5281 gtacatacgg ttgaatactt gacgagaaac tttcccttta cggaatccag gtacgttcaa
     5341 gctttttttc actttattaa acgcagttgt taatcctttt tggatttcgt cttgcgcaat
     5401 cgtaaatttt aaaacaccat cattcgtgcc ttttttttca aatgttgcag tcatttaatt
     5461 ccctccgagt ttagaaattc attttataca tcaaaaacgt ttcatgcata cctttaaata
     5521 gtacactaac cctttcaaat tgtaaacata ttgaatggat agaatccttg tttttcataa
     5581 catttttatc tttttcactg ataaaatcta atggaagaag atcatttgct gcaaaatttt
     5641 ttctcttttt tttaaaaaat cttgtttttc atcttctgta ccttcataca tcgtttttcc
     5701 tgtacacgcc tctacagtca gttgaaacca gcgcttcggg tcttcgatgt atgtatcata
     5761 gacaggataa acaattgcag attgcaacgt gaattcttct tttaacacgg aactaagcag
     5821 cgcatcttca ttcccgtaac gatctgaaaa tacacataat tgctggtaac ttgcctgctg
     5881 ctgcggcttt ggcagatctt ttggtatgac tttgactaac ttttcttcaa tcgtcaaata
     5941 gaaaacttga tggcattcgt tcaattgcct aagtgtctcc agtaattttg cccttgctaa
     6001 aatatggacc tctggcgata caataagttt actcgctgtt tggataaact catgataagg
     6061 aagctgttcg atctcatcaa tagacgcaag ctgacgtgca ggttccaacg aaggaagttc
     6121 ctccgattta ttgtggagtg ccttgatctt ctgcctctcc atttgtccgg ataattcttc
     6181 tgcaaattgg attttttcaa tgacccgctg ctcgaaagct tcataaaaat ctgtacttgc
     6241 tatcaatttt ctagcatata aaaaatcttg tgtactgatt aacaagtcaa aataaaattc
     6301 agcaaactcc tcattttgaa gatactgttt ttcatgcaag agtgcttgct tcaatgcttc
     6361 ttttttctcg tccaactcgt aaagacaaaa aacaagcagg ctattagcct caaagctttc
     6421 tttcaactcg taagctcgtt gaaataattc ttttgcctgc tgatattgct ggtttgctag
     6481 tgcttgttgt gccactcgca ttaaattctc atatttttct ggaaaatcaa tcggcttcac
     6541 gcagtgtcct cctcttgctc atattcagct catgttcaat agtagcaaaa caaaaaaagc
     6601 atgacaatta aaaataactg aagcaccctc taaaagctag attttagatc tgacttttag
     6661 aaggtgcttt caactgttac tgctttttat tgctcatcag ctgttgactt cctggttgaa
     6721 cgctttgatc agattgatcg taacaacact tgtattagtg aaaaaatcgc ccagatgttg
     6781 ctttttctga ttaaagactg ctggaagata ccctaacatc agcaaaggaa attttagaat
     6841 gaaccggcaa gcaccttctc taactaaaac ggttgaccag ctaagttctt cttcatcaaa
     6901 gctgatgact cgaataccaa agatcatttt cccaatcgtt tggccatgat tgaatttcgt
     6961 caataaaata aaatacgcta gataaataag taaggctgtg aatccgtaga gactcagata
     7021 agaatctgaa aaattaagcc aacctagatt tttggcaata ccaagtgtta aacgcgtaat
     7081 agctccgata cacaaaagat cgaccaagaa ggcaaacatt cggataaaaa aacctgcata
     7141 gaaatactta ggaaaatcat ttgcttttgg cggttcattt cttgtgtccg tatacttttt
     7201 ccatttacgt tgtttctttt tgatttcttc gggcgtcaaa ggctctcttt caaaagagct
     7261 cagtaccttt tgggaaaagt tggagtcagg tcctacagca ggctgctgtt gcttatcttt
     7321 ttcttgttcc ttattttcat ttgaaagctc tgacatgctt actcacctcc gtaataatac
     7381 attgcttttg gtgattccgc tgtccctaga ttctcaagaa cggataagat ctgactagtc
     7441 tcagaaggct tcaatccctg gaattctgct agtttacttc ctaaccatgt actagcaaac
     7501 ccagatggcg tggaagaata ttctactagt tccgcatctt caagctgttg ttcctttcgt
     7561 atagctttta aagcatcttc aggaaatccc aattcatcga ctaaacctac ttcttttgct
     7621 tggacaccat cataaatcct tccatcagca atttttttga cttcttcttc agatttattc
     7681 cgtccctcgc tgacgattgc tacaaaacga ctatatgcgc tgtcgatata tgcttgaagc
     7741 accgcttctt cttcttttgt ctctggtcgc gttcctgaac ccatatcttt caacgctccg
     7801 cttttaactg ttgtatcttc cacacccagt ttttctagaa gtcctgaata attcaatcct
     7861 gacatgatca caccaattga gccggtcact gtttcttctg ttgcaaagat tttatctgct
     7921 tgagcagaaa tatagtagcc accgctagct gccatgtttt tcatgctgac atacattggg
     7981 atatcatgtt ctttacgaat cgtatccaat ttcttagcga tttccgcact ttcgtagaca
     8041 ccgccaccag gagaatttac ttctaaaaag atacctttga ctgttggatc ttcttcaatc
     8101 gccttcagtt gttccataaa atttttatga ttgtaacctt cagaagcaaa taaacctgaa
     8161 gaccctcccg cagcaatcgt tccatctact gataatttta cgattttttg cgtactgtct
     8221 ccctcttcca acacatttgg agctagttct tcatccccat ataaccaact gttcaatccc
     8281 tcaatttgat cgttttcctg aggcttagtc aaactagctg aaactaacga aaccacaaat
     8341 aatcctgcag caatcagaac tgctgcccaa cgtcttttat tcattctttc tcctttctaa
     8401 tacaagtttt ttatgacgat cacagtttct gactgctcag aagtgtcgaa ccgctctgct
     8461 ttttcttccc gcgcataccc ggaaaacgac tcttttgtta attcatcttc taccgtaaac
     8521 tcacgagctt gagcaacttc caaggcatca ggtgcgattt cttggatgct atcccagcca
     8581 gtatctaaaa atattttcgt atcatctgat acaccagctg atgcttcgaa tttggacaat
     8641 tgttttttta acattcccag tgtcatgatc tcaatttttg aatccatttt tactcctcct
     8701 ctatccgatt actttctatt gtaccgaact cctgaaaaag atgatagata aaaacccatt
     8761 attttttgac ataaaaagga catttgacaa agaatttgat atataccaat ttatttttta
     8821 ttcttttttt gttgacaaga acattcgcaa aaggttatga ttttaaatgt ttccagataa
     8881 aaaacaaaaa ggaggatagt tatacctttt aggatagcgc caggtctgtg taaacagatg
     8941 acgaggagag ggtttatcga aagattcggc ggatggccct agggactgca ctctacagaa
     9001 aatccacaaa aaatgtctgc aaagataaaa caaaaggatt ttcctgcagc tggaagcatt
     9061 catacgaatt cattatagaa agtaggatgt tttttatgtg cggaattgtt ggaatgattg
     9121 gattaaaaaa tgtaacacca ggattgattg acggtctaga aaaattagaa taccgtggtt
     9181 atgattcagc tggtatcttt gtctcagatg gcacaactga ttatctggtc aaagcacaag
     9241 gcagaattca aaatttgaaa aacaaaataa atattgatac tacaggaaat ataggtattg
     9301 gtcatacacg atgggcaact cacgggcaac cttctgaaga aaacgcccat ccacatactt
     9361 cccaaagtgg acgcttcgtg ctcgttcaca acggcgtaat cgaaaacttt gaagaactta
     9421 aaattgctta tttagcaaat gaccacttta ttggtgagac cgatactgag attatcgctc
     9481 atctaattga aactttcgcg aaggatacta caacgcaaga ggcgttttta aaagctttgc
     9541 gggtcatcaa aggttcttac gctttcgctt tgattgatcg gacagcacct gatgttattt
     9601 acgtcgctaa aaataaaagt ccactattaa tcggactcgg cgatggattc aatgtgattg
     9661 ccagcgatgc aatggctatg cttgctcata cgaaggaatt cgttgagatt gaagatgaag
     9721 agatggttac cgtcacatca gaaaaagtta ttattcaaaa ttttgcaggt gacatcgtag
     9781 aacgttcttc attcgaagca caagtagatg cttcagatat cgagaaaggt acctatcctt
     9841 tttatatgct gaaagaaatc gacgaacagc ctattatcat gcgtcgtatc gctcaaaaat
     9901 acgtaacaga agacaatcgt gtctctcttg atcaaaagct ggtcgataca ttgtctgata
     9961 gcgatcgaat ctatatcgtt gcttgcggaa ctagttacca tgcggggtta gcaggtaaac
    10021 agactttgga aaaattgaca cagattcctg ttgaggttca tttagccagt gaatttggct
    10081 ataacacacc tcttctttca aaaaaaccat tctttatttt cttgagtcaa agtggcgaaa
    10141 cagcagacag ccgccaagtg ctggtaaaaa tcaaccgtct cggctaccct tcacttacga
    10201 ttacaaatgt tgctggttct actctttcgc gggaagcatc ctttacactg ctattgcatg
    10261 ctggacccga aatcgcagta gcatcaacga aagcttatac tgcacaaata gctgtactaa
    10321 ctttattagc caaagctatc ggtgataaaa aagaatttga aacttctctt gtattcgata
    10381 tcactcatga attgagttta gttgctaatg cgatggaatc ggtcatcgcc caaaaagatt
    10441 atttagaaga gttggcagaa gtatatctca gcgatacaag aaatgccttt tacattggac
    10501 gtggtgctga ctacaacgtt tctcttgaag ctgctttgaa attgaaagaa atcagttata
    10561 tccaggcaga aggttttgcc gctggcgaat tgaaacatgg aacgattgct ttgatcgaag
    10621 aaggaacacc agtgatcgga atcatcagtg aagaagtgac tggtgcgcat actcgaggaa
    10681 acctgaaaga agtcgaaagc cgtggcgcta aaacactagt tattgtctca gagggattag
    10741 aaaaggaaag tgaccagctg gtgctaccaa ctgtccaccc ttatctaact actctaacca
    10801 cagtcgtacc gactcaatta cttgcatatt acgcaacgct attacgggga ttcgacgttg
    10861 acaaaccacg taatttagcg aaatcagtga cagtagaata ataaaagaac aaggaccagt
    10921 gtcagacatc tgacactggt ccttgttctt ttatttttta tgaaaatatc tcttaatcca
    10981 gtccgatttc ttctttgact actgccgcga ttttatcgac atagtaatta actttctcat
    11041 cagtaggcgc ttctgccatc actcgaagta atggttctgt accggaagga cgaactagga
    11101 ttcttccttc tgatcccatt tcagcttctg cctcttcaat cacttttttg attgccggaa
    11161 catccatcgc accgttttta tcacttacgc ggatattcac taatttttgc ggatagattg
    11221 ttacctctgc tgcaagctct gataatttct tacctgtctg tttcatgata ttcagtaact
    11281 gaattcctga aagcatccca tctccagttg tgttaaaatc taagaaaatc atgtgcccag
    11341 actgctcgcc gccaaagtta taatcgtttt tgcgcatttc ttctacaacg taacgatcac
    11401 caacttgtgt aacgacatct ttcaaaccga ttccttccac cgctttgtgg aatcctaagt
    11461 tactcatgac agtcgtaacg atcgtatctt ttttcaatcg atttttttct gccaagtatt
    11521 tagcacagat aaacatgatt ttatcaccgt cgacaatgtt tccaagttca tccaccgcaa
    11581 taatacgatc gccatcgcca tcgaaagcta gtccagcatc tgctcctttt tcaacgacca
    11641 tctcagctaa acgttctgga tgggtagagc ctacgccgtc attgatattt aacccgtctg
    11701 gatttgtgcc cattgtataa aaatctgttt caagatcggc aaacaaacga ttaacacttg
    11761 ttgaagttgc tccgtttgct gcatcgacac aaatggtcaa accggaaagg tcaccgttaa
    11821 tcgtttgtac aaggaattga gaatatttca ataagccttc tggaaattct tctacagttc
    11881 ccaatccttc agctgatgga cgaggcaatt catccacttc agcatctaat aatgcttcaa
    11941 tttctagctc ttggtcatct actagtttaa aaccatctgc accgaaaaat ttgattccat
    12001 tatcttgtgc tggattatga gaagcagaaa tcatgacccc tgcgcttgct ttttgcaaac
    12061 gtgtcagata agcaacacct ggtgtagaga tcacacctag ctggaacact tcgataccca
    12121 ccgataaaag tcctgaaatc agcgcttctt ctaacatttg acctgagatt cttgtatccc
    12181 gccctactaa cacgcgtggg cgttttcctt cttcttcatg ctggcttagg acataacctc
    12241 cgcaccgtcc cagcttaaat gcaagttctg gcgtaagttc cttgttggct tcaccgcgta
    12301 cgccgtctgt tccaaaatat ttacccatag ttgtaaaatt actcctttaa cttaattttc
    12361 tgaggaattt gttctgctca tttgtgttga ttcgcttgtt tctgatgttt cttctgtact
    12421 tgtggtcgag ctgcttgtcc cagattccga actggaagaa atggttgttg tcgatggttc
    12481 cgttcctgaa tcagcagatg aaatacttct actcgtactt gtattttctt gtgtccgact
    12541 gctcgtcgaa gcagagctac tgcttgacgt ttgcaacacg ggtgtaatct ccacactaac
    12601 tgattcgggc ttgatactta acccctcttc tactggtatt tcaattttac gagttgttcg
    12661 atgattgata cctgagacat tgatcggtac agcaatactg tcaccgatcg atgcaagcaa
    12721 atcgctagag ccgcttactt ctgcttccag ttttgaaaaa ctgaaatcat agctttctac
    12781 cccggcagct ggtgttcctt gctgtatccc ataaagtcgt accgacttgc ttggcttgat
    12841 cgaatcagct ctcactgtta cttgcactgg atcagagacg atacttaata cttcccctgc
    12901 tgcattcagc gcctgtattg ggacctcact attaatccca tcatctgtga ccatcgaagc
    12961 gtccacatca gctactactc gtttgatttc tgccaatgtt ttgtcacctg tagtgataga
    13021 gactttttta ggactaacgg aagtatcatc aagttcatat ccagatggtg tgaacgttga
    13081 aggaatcaac gtttccacat cgaaatcttt tattaccttt ttctcaaccg ttactgtaat
    13141 agtcgatggt tcgatctttg ctgtgacagc actgctcaaa ttttgaattt ttagcggaac
    13201 ttcatgtgtt ccttcgccca gcttagtgat atctgctgtg acgcggaaca tccgtgtatc
    13261 ttcgtttgcc tctgcattca actgaactcg gtttgcactg gaaagtttga cagtcactgc
    13321 attttcatat ccatggatat agtagcgctt tgaatcataa gaaatcttga tcggcacccc
    13381 cgtaactgtt tgattatacg cttcatttcc agaaagcgta tttggaacgg actggccatt
    13441 tgcattgaag tacagcgcaa tactgaacag caatgcaata aaactgtaaa gaatgcttgt
    13501 ccgtttctct tttgagatca tttgcgatcc cctcccttag tcataccgtc taggaaagat
    13561 tgaatcacgt ttttcttgtt ttttgttttc tcttcagcaa ttagttctct acgcaagatt
    13621 gccaaatatt cttgctggga taagccagcc atcagttcat tatctaacgt gatgctgacg
    13681 tccccggttt cttcagagac aacgatggta atcgcgtcac tgacttcgct tataccgaca
    13741 gctgcccggt gtcgagtccc gaattcttta gggatcaaca tactttctga tagaggaaga
    13801 taggcgcttg ctactgcgat ctttccttct tttacaataa ctgcaccgtc atgcagcggt
    13861 gtattaggaa taaagatatt gatcagcagc tccccagtga tatctgcatc taaagcaatc
    13921 cctgtctcaa tatactcatc taatccagta tgacgttcga tagtgatcaa cgcaccgatt
    13981 tttcgcttgg acatatattg gatggctttg tcaaaagaca agatcatctt ctcatcttcc
    14041 tgctgttcgc tttttgattg tttgaaaagc gaactgcgtc caagatgttc cagtcctctc
    14101 cggatttccg gctgaaaaat cacgatagca gctatcaccc cgtacgtaat gacttggttc
    14161 atcagccaag acaaggtgtg cagaccaatg atttccgcta cgattcggat aacaataaaa
    14221 atagctacac ctttcagaag ctgaatcgct tttgtgcctc gcagcagttg gatcagtttg
    14281 tatacaagat accagacaac cagaatatca agtatgttca ccaaaaaatt cagagagaac
    14341 aagtcaccgg aaaacatttg ctgccaatag tccaaattaa aaagttcccc gatttgaaat
    14401 gacataaaaa gcctctcttc tcttttgaga t
//
LOCUS       DBKOIJ010000062        13826 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000062 DBKOIJ010000000
VERSION     DBKOIJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.064"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            20..868
                     /locus_tag="ACV5NJ_001485"
     CDS             20..868
                     /locus_tag="ACV5NJ_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HHY6485583.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1039..1380)
                     /locus_tag="ACV5NJ_001486"
     CDS             complement(1039..1380)
                     /locus_tag="ACV5NJ_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HHY6485584.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1466..2173
                     /locus_tag="ACV5NJ_001487"
     CDS             1466..2173
                     /locus_tag="ACV5NJ_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HHY6485585.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2160..2483
                     /locus_tag="ACV5NJ_001488"
     CDS             2160..2483
                     /locus_tag="ACV5NJ_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HHY6485586.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2572..3609)
                     /locus_tag="ACV5NJ_001489"
     CDS             complement(2572..3609)
                     /locus_tag="ACV5NJ_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HHY6485587.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4056..5039)
                     /locus_tag="ACV5NJ_001490"
     CDS             complement(4056..5039)
                     /locus_tag="ACV5NJ_001490"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HHY6485588.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5455..6225)
                     /locus_tag="ACV5NJ_001491"
     CDS             complement(5455..6225)
                     /locus_tag="ACV5NJ_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485589.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6218..7153)
                     /locus_tag="ACV5NJ_001492"
     CDS             complement(6218..7153)
                     /locus_tag="ACV5NJ_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485590.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7747..9396)
                     /locus_tag="ACV5NJ_001493"
     CDS             complement(7747..9396)
                     /locus_tag="ACV5NJ_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS-related protein"
                     /protein_id="HHY6485591.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9591..10487)
                     /locus_tag="ACV5NJ_001494"
     CDS             complement(9591..10487)
                     /locus_tag="ACV5NJ_001494"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHY6485592.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10480..11160)
                     /locus_tag="ACV5NJ_001495"
     CDS             complement(10480..11160)
                     /locus_tag="ACV5NJ_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHY6485593.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11161..11943)
                     /locus_tag="ACV5NJ_001496"
     CDS             complement(11161..11943)
                     /locus_tag="ACV5NJ_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHY6485594.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(11936..12853)
                     /locus_tag="ACV5NJ_001497"
     CDS             complement(11936..12853)
                     /locus_tag="ACV5NJ_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHY6485595.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(12856..13140)
                     /locus_tag="ACV5NJ_001498"
     CDS             complement(12856..13140)
                     /locus_tag="ACV5NJ_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485596.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4074 a         2974 c         2436 g         4342 t
ORIGIN      
        1 ttcatgaaag gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga
       61 acgcggttac agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta
      121 cttgtccgca acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa
      181 gaccaacatg acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc
      241 tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga
      301 actctcgtcc cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat
      361 tcttatagaa catccgcact ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat
      421 aaattattca gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa
      481 caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt
      541 tgaatcgctt attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat
      601 tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga
      661 ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca
      721 aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta
      781 ctctataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa
      841 aatagataaa aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta
      901 aacgataaca accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga
      961 atcatgcaaa ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta
     1021 ttaggtacgc gttttgtttt ataagtcatg acaaatttcc ttcgttgctg gatagatttt
     1081 ccggcattgt tgatagattt tatgatactt ttctacattt tgtaagagag gttccgctgc
     1141 atctgtatat tggataaaat tctgacaaca actttcaggt gtgtcgaacc accctaaccc
     1201 tagtgcagca atcattgttg cgcctaatgc aggtccttcc tccatactta aacaatagac
     1261 cgttgcatcg aaaatatcag cttggatttg tagccatgca agatttttcg cgcctcctcc
     1321 caccgaaaca atacgtgtaa atctgcgacc cgctagttct ttacgtttca atcccaccat
     1381 tgacaaatgc tcattaaaaa tttagaatat tcacaagttt aacgacaatt atgaaaattt
     1441 atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa
     1501 agacacgtta cctaccgttt tcggttatat cggtattgga cttgcatttg gtatcgttgg
     1561 gaaagctgcc ggatttcatc cactagtcgt cacgttgatg tccctgctgg tctatgctgg
     1621 ttctgcccaa tttatcacag tcagcatgct tgctagtcac agcccattgc tttccatcgt
     1681 tttctcgacc tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta
     1741 tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag
     1801 tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat
     1861 gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact
     1921 tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat
     1981 gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca
     2041 gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag
     2101 caacttagtg attgtagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca
     2161 tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt
     2221 ctagggtcct accatttgtc ttactgaaaa aattcgattt accgcaaccg ttgcttgagt
     2281 atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc
     2341 aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac
     2401 ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggcagga attctttccc
     2461 tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg
     2521 cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt
     2581 gaacggacca ctaacaacga cttttcctaa catatgtcct gtttctacgt ctttggttgc
     2641 ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat
     2701 aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc
     2761 atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt
     2821 ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg
     2881 tcggacaagg tgcttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata
     2941 ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat
     3001 tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta
     3061 gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccggatcc
     3121 gttgatcact aagatttttt tcccgcgatt ggcattttct tctggagtta gaccaaattt
     3181 ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcag cttcttcatc
     3241 tgaaagattt tttggtgcaa gagcgacaat acgttcgtca actagttggt attcttgatt
     3301 gcttcctgct cttgttgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg
     3361 cacttcgtca cctacagcaa caactttccc gactccatca aaacctaaga tacgtaaagc
     3421 atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt
     3481 gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa
     3541 gctttttgga tcatcgatcg gcaacccttc ataaaaaccg actaccttca ttaattcctt
     3601 ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac agtgttcatt
     3661 tcaatatata tagtatgcta tttgttccac cttttttatt catcataatt attctcttac
     3721 ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca
     3781 caacctctta taaacggtgt tctcctacca actcttcggt cacaagtcaa aatattcatc
     3841 aaacatgaga agctgttttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc
     3901 acaacctctg atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga
     3961 caaaatatga agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt
     4021 tcacgacctc taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga
     4081 tgttcttttt caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc
     4141 aactgaactc gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga
     4201 tgtagtcctg tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc
     4261 agacgaagta gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca
     4321 taaggattct tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc
     4381 aattcttctc ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga
     4441 gcgatttggt cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta
     4501 ctcatctctt tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg
     4561 tgactgatcc attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg
     4621 acaggcaaac cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg
     4681 atcaagccac tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata
     4741 tcgattcctt ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc
     4801 ttgtctcccc aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct
     4861 cgtacactgt cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag
     4921 aagacatccg gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct
     4981 tccattggtg ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt
     5041 tatttgttac tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc
     5101 attatactag gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga
     5161 gcaaaaaaaa gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt
     5221 caaaagcttg catttgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     5281 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     5341 tgttcaatca gctgaccctc ggtattcgct caaaaaacaa caaaatatga ggaactattt
     5401 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     5461 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     5521 taacacaagt acgatcatcg atgttgctac tggcacatcg atcgttccaa ggaatccgta
     5581 acggaaaccg gagatcatat aaacgatcgg attgactttt gaaacagctt gccaaaaggg
     5641 cggtaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     5701 tgtaggcacg atcgatacat catcataaga ctgagcaaaa atcccgttga gcagtcctgc
     5761 taatgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     5821 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
     5881 acccacacca ccaatcacaa agccccaaat aatcacatgc gtaggaacag gtgctaccaa
     5941 gatttcttcg atatttttct gaaatttttg agagaagaag gacgaagaaa cattggcata
     6001 cgaactagtg atagcagaca tcataatcaa accaggaacg atgaattcca tatatgaaaa
     6061 accacccatg tcaccgatcc gcccaccgat catttttccg aaaataacaa aatacaagga
     6121 tgtcgtgatc actggcggaa ccagtgtttg gacccatata cgcaaatagc ggttcgtttc
     6181 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
     6241 gattattttc ttctgtgatt ttcaagaaca gttcttctaa acgattcgac ttattcctca
     6301 tagaaagaac tttgattcct tgtcttgtaa gctgatcgaa gattccgttc accccttgat
     6361 ttcgttcaac ttcgacagcg attgtctgct catcttcaaa tgtacaagca tatccttcaa
     6421 tcacaggttt ggtatcatat ggcgctaaat caaagataaa tgtttcaaat tgcagttttg
     6481 acagaagctc tttcatactt gtattctcga tcagttcacc cgattggatg attccgatgt
     6541 tgcgacaaag catttcagct tcttctagat aatgcgtcgt caaaatgatc gttgtaccat
     6601 tttcgttcaa ttctcgtaga aactcccaca tctcgcgtct caactcgatg tcaactcctg
     6661 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
     6721 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
     6781 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
     6841 agcctgcttg attcacgacg atttgctgga ccgtttcaaa agggttaaaa ttgaactctt
     6901 gtggaaccaa tccaatctgt tgctttgctc gaactagatc cttgtccaga tcataaccga
     6961 agacattcac ttttccagat gttttattga ctaaagaagt aataatcccg atcgttgtag
     7021 acttcccagc tccatttggc cctaatagcg catagaaatc tccttctttc accgaaagat
     7081 cgattccccg caatgcttcg accccacttc catagatctt tttcaaatta tttatttcaa
     7141 gcgcatttgc catttatttc ctatctcctt atagacatga ttcttaattg tatcatggaa
     7201 tacagcagat gaaaaaacaa cttggcttcc acacaaaaac acacctgcta acaaagagga
     7261 gatgtgtttt ctagttcatg atcatcttcg atagaataac tatctaaaca actctggaac
     7321 ttttaaaatc ataaaagtac cgacagcaac tactacgatg acatatagaa gatccttata
     7381 actgaggttc ttgtcttttt tattcatgtt aagctttcct ccgtcctttt atcgaaagct
     7441 tttttctttt attttaaccg aaataaataa aatggaaata gttattttca atttctgttt
     7501 tcgctgtttg ttttacggag aactttgcca tggaacgtgc gtctcgtcgc tgatgctaag
     7561 ttttttcgat aaaagctgct agaaggaaga cagcgtggtt tattttgtat cgtcttttgc
     7621 taaatcttcc aagatcatct ggacagcaga ctcgttccca ttgaattctg ccactttttt
     7681 ggcatcaatg tacttctttt gataatcgtc tttttggacc cacgcatcat gcttcgtttg
     7741 ataaagttaa tcaaacggat accactgacg ctgttcatcg aacaacaaac tttctttttg
     7801 ggcctgttca actatggatt taggataatt caaaactttc agcaaggcaa tcgcatttcg
     7861 ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg attccttttc cttctgttac
     7921 ttgttcttca aagtggacat tctcacaata attctttaaa atctctgtca attcgatatc
     7981 atgtgtggcg acaaatgcta aagatggata ctccgcaagc caccgaacga ctgaagcgga
     8041 agaagcaatc cgttcgatcg tatttgttcc ttttaaaatc tcatctacga agcagtagca
     8101 gcgttctttg gaagccactt ggtccagcaa acgtttgatc gatttgattt ctgaaacaaa
     8161 ataactgtcg ccttcaaata aatcatcttc gattgccatg gaagtcaaca catgtccagg
     8221 ttgcagggta aactgttctg caatcgctgt ttggatcgtc tgtgctagaa tacagctgat
     8281 ggctatactt ttgacatacg tagattttcc tgatgcattg gaacccgtga ctaatgtatt
     8341 cttctgccag ttgactggat taactaccgc ttcttttaac aatggatgat agatatcttt
     8401 cgctgtcaca ccgccctcag taaactctgg tcggcaagtg atcggcatat aggtgcgata
     8461 attcaaaacg gctgctgcaa cttccaattc tcccagcaat tcccacagct tgatggcttc
     8521 agttgaatgc ttttctaatt tagtgagaac gaaatgatag gagataaatg gcagcatgaa
     8581 catgatgttg acatattcaa aaatcaattc tgcttcactc ccgctcttca ttcgaaatga
     8641 aaaacctaat ttagggatgt ctcttaatgg tttagtttgc tgtttcagct ctttttgaag
     8701 tggtgtcgtc atttttgcta actgattggc ggctgaaatc gttgaaacca aataactcat
     8761 gctattcagc tctgtttcta gtgtttgttt cttcactaga taatagaccg tattgaatac
     8821 taagccaccg attgctagat agattcctag tacttgccct aaaagaagca aacctacacc
     8881 caccagcgga agcaagccaa gaaaaataaa taaacctacg cgtccaatct ttttactttt
     8941 tccatccgct agatattgtt ttgcaaaatt atgatctttt tttcctaaac gagcgaattg
     9001 atattggatt ctttctctca gttgaggatt ttcctgataa aaagcaatta gtttttctaa
     9061 ctgataatct tctccaaaat caaagctacg taaacgctga tacaacgctt ctgaacctac
     9121 actagaatag gtactgttga tcccttcaaa tacttcgaac atatccaagt cataccaagt
     9181 aagatcgtcg atttcactat cccatgaacg gaattttttc tccgtgagcc acgcatcctt
     9241 caaactttct tctttatcaa atctcggttg ataaggaatt tttccccaat tactacgaac
     9301 tgtttccttt actttcaaac gtgaaaaaat gtctaaaaca actaataaaa taacaactgc
     9361 aataataatt aaaacgataa attctctttg gcccatatcc ctctccgttc cttgatttgt
     9421 tagttttagt atagcactgt tgcgctattt gaaaaggggg tacaaaaata actgtcttcg
     9481 ttacctaatt cgtatcaatc acaccaaaat aaaaacacct tccaaaaaag aagatgtctt
     9541 taaaacgaga tacaattttt tgtcattcac ttcaggtaaa ttagagccaa ttaaaccctc
     9601 cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat ctctcctccc
     9661 attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc cgtatttgtg
     9721 cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt ttgcgctcct
     9781 ggtttgatag gattggccac tgttaatcga atgaatgaat cctcttttga aaatgtcacc
     9841 tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag cactcgcagc
     9901 aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa cgctacctcg
     9961 atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac ctctttagaa
    10021 aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc aagatcataa
    10081 aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt taaatacttc
    10141 tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt aatagcagtc
    10201 aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt ttcttccatt
    10261 tcttgaaatg cttgtccttt ttgttcattt tcatccaccc atgtattcag tgcatctact
    10321 aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata ttcttgtcgt
    10381 tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag gaagaaaata
    10441 aaacttccca taaagataac aacgagaagc aacagccact taatcaaatc taaaccccac
    10501 tccccacacc gtatgaatat acacagcatg tgcctttgct agtttccctc gaagattgga
    10561 aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa ctgtttcata
    10621 aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca gcagatcgaa
    10681 ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg actctttgtc
    10741 cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga cagaagtctg
    10801 atgacgaagt tggatgccga ttcgtaacaa cagttcttcg atatcaaatg gtttcgtcac
    10861 atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc tttttgctga
    10921 aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg cttcacctga
    10981 aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc ttaacaataa
    11041 ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa gaacctcctc
    11101 cagtaaagaa tgaacagctt catcgtcttc gataatcaaa atagtttcca gcaggaacac
    11161 ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata ccacgattgt
    11221 tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt gcgcaaagtc
    11281 cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc ctttgatttt
    11341 tgcgaaaaca ccgggaacga aggccgttcc catgatataa agaaaagaaa gaagcaaact
    11401 gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg tcgctaatat
    11461 cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt tgattccctc
    11521 aacttcacca aatccgaaag caagtgtgta agcaatcatg gctagccccg taatcaaaac
    11581 cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat aagtcattcg
    11641 attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt ccccaatata
    11701 aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt ccaccattaa
    11761 aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat tgccgtattt
    11821 tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca tcagtcctag
    11881 tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta atagattaaa
    11941 catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc ccaactaggt
    12001 ggataccatc cacaggaact tttgcctcta ccaatgcttg attgatggct gctacttggt
    12061 caattccttc aaaaagctgg ataacacggc tagattggac aaaataatcc ctataaccaa
    12121 gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt ataaattgac
    12181 gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct tcatgcaaaa
    12241 taccgtagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa agcaagacgg
    12301 tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc atccctgctg
    12361 gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg atcagtgcat
    12421 ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc tttttccctg
    12481 tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac acaccgccgt
    12541 tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga gcaggggttt
    12601 caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca aataattcga
    12661 tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta gacttacccg
    12721 ctccattctc cccaatcaaa ccatagatca ttccacgttt gatttcgatc gatactttat
    12781 ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag accgtttcat
    12841 ttccaaaatc catacttatc cttctttctt attgataagt gaagtatagt tgacgggtat
    12901 aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa tttcaagaaa
    12961 aatcagactg tctcgcagac gtggttgagt tgtctgccaa tcaaagagcg caccaaacag
    13021 tgctaatcca agtggactgg ccaccatggt aatcgttgtt atcgttgaca tcagacgtcc
    13081 ttgtatatgc gttggaactt ctcgctgaag ccagaccata taaaaagcaa aagaaaacat
    13141 taatccttaa aaacggactt atgaaaaata atacatgccg tttgggattt acaattagct
    13201 accaaataag aggttatgac aaaagacttt tcacaatccc ttcttccgaa taaacggtgt
    13261 tctcctgcca actcctcggt cacaagtcac aatatttatc aaacatgaga agcaattttt
    13321 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcag aagcggataa
    13381 ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg aatgatatta
    13441 tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac tttccaaatt
    13501 gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc ttcgcccagt
    13561 acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg agaactgtga
    13621 agctttacgg gctttcttaa gacctggtta aacaaaactc tattttattg cattttacag
    13681 gaatgtattt catgcgaaat taataagtct ttgtttatag agttttacta gggtataaaa
    13741 agttgtatgt tatcattacc tcaaaacatt acctcagctt ggcgagttgg ttgattgaag
    13801 aataaatcat ttatctagat atctca
//
LOCUS       DBKOIJ010000063        13770 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000063 DBKOIJ010000000
VERSION     DBKOIJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.065"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            239..577
                     /locus_tag="ACV5NJ_001499"
     CDS             239..577
                     /locus_tag="ACV5NJ_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485597.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(782..1344)
                     /locus_tag="ACV5NJ_001500"
                     /pseudo
     CDS             complement(782..1344)
                     /locus_tag="ACV5NJ_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2076..2657)
                     /locus_tag="ACV5NJ_001501"
     CDS             complement(2076..2657)
                     /locus_tag="ACV5NJ_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6485598.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2840..3466)
                     /locus_tag="ACV5NJ_001502"
     CDS             complement(2840..3466)
                     /locus_tag="ACV5NJ_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HHY6485599.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3488..4819)
                     /locus_tag="ACV5NJ_001503"
     CDS             complement(3488..4819)
                     /locus_tag="ACV5NJ_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HHY6485600.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4974..5375)
                     /locus_tag="ACV5NJ_001504"
     CDS             complement(4974..5375)
                     /locus_tag="ACV5NJ_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HHY6485601.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5461..5667)
                     /locus_tag="ACV5NJ_001505"
     CDS             complement(5461..5667)
                     /locus_tag="ACV5NJ_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485602.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5762..7264)
                     /locus_tag="ACV5NJ_001506"
     CDS             complement(5762..7264)
                     /locus_tag="ACV5NJ_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HHY6485603.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7282..7737)
                     /gene="argR"
                     /locus_tag="ACV5NJ_001507"
     CDS             complement(7282..7737)
                     /gene="argR"
                     /locus_tag="ACV5NJ_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HHY6485604.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7835..8452)
                     /locus_tag="ACV5NJ_001508"
     CDS             complement(7835..8452)
                     /locus_tag="ACV5NJ_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HHY6485605.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8673..10046
                     /locus_tag="ACV5NJ_001509"
     CDS             8673..10046
                     /locus_tag="ACV5NJ_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6485606.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10121..11233
                     /locus_tag="ACV5NJ_001510"
     CDS             10121..11233
                     /locus_tag="ACV5NJ_001510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHY6485607.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11305..12942)
                     /locus_tag="ACV5NJ_001511"
     CDS             complement(11305..12942)
                     /locus_tag="ACV5NJ_001511"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HHY6485608.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13110..13718)
                     /locus_tag="ACV5NJ_001512"
     CDS             complement(13110..13718)
                     /locus_tag="ACV5NJ_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HHY6485609.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3718 a         2893 c         2459 g         4700 t
ORIGIN      
        1 aaaattcccc tttgtaaact atctagaaaa ttgttttgtt taaagtatat cttattggat
       61 ataaatagaa ataaattgta gccagttcat tttttcttta actaaaaaaa taatccaaga
      121 aacattgaac ctatggatcc aaaagctacc caaatactat agctaacccc ccgctacctt
      181 caattttgca gataaaacgc tttgggagaa aatttcaaag acaacaggcc atcctatcat
      241 gcccgattat aaacaaaaag ctggctattc cttttgctct gacaaatttc aattgggaaa
      301 gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg atcaaagaat gcccgcttca
      361 aatagaaaca ttggttcaac gaatttcaac tagacaagac tttgctattg tagaacgtga
      421 gatcgtagct atttttgtag aagaagggat tttacatgat ccaactcata tcaatgtgga
      481 taaatggcaa cctcttatct ataaattcag agaatataca actgccaacc agtcgttaac
      541 tttgaatttt agattccaag aattcaaaga gtcataatga taaacatcgt taggtataaa
      601 taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc tatacattag aaaggtcgac
      661 tgtgacccta tagatgcagt atatgcagct tatgagcatt tcttctgttc tctttgtttt
      721 tgggatctgt gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa aatcgattat
      781 gttatacctt ctccattact aattcaggca tctgctgagt aatgattttt cgcgcaaaag
      841 aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc attcggtcat
      901 ggattggtgc gactgtgcgg ttaggttcag tagtcattat gattgattct gccatttttt
      961 catcctgatg gctacgataa aaaccgccaa tatacactat tttttcctta tttgaaaaca
     1021 taaatttgtc tttttcttca ttccactcat tgggaaaaca caacgatttt gcaaaaatgg
     1081 ttgatgaaag gtcttttttt cttctactgt ctctgcacgg gcattaatca tcaattgtcc
     1141 ttttttaaat ccggtaaatc cccacttcat gatttccgga gtgaggatgt tttcttcctc
     1201 ttttcttaac gtcacaacaa gatttgacgg aaaaacttct ccagctgcta attctgctgc
     1261 ctgcttcggt tgtttcgtct gggcttctcg ataataattc tgaaggagct ctgactctag
     1321 atcaaaaaaa taacgaccac acatcctaat ccctcccaat ctttttttct atcatacact
     1381 gcaacaagaa gttcgcacca acaaaacgct ttcaattttt gtagaactaa aaaagttgtg
     1441 acaagtgaca aactctccag tgaatggtat tctcctgcca actcctcggt cacaagtcac
     1501 aatattcaac aaacatgaga agctgctttt tgaaattgtt ccttgtgact cggagtgaaa
     1561 cggcaggctt acgacctctg ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc
     1621 aaatttatgc aaaaatgtag aaagctattt tcacaaaatt tttcttaata ctcgaaacta
     1681 agcgactttt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata
     1741 agcccaacca aacgaaaaat atgagcagct atttttattt ggctgttctt attacttgat
     1801 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagcta accttcggta
     1861 ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca
     1921 ggtcaaagcg ctgatttcac aacctctaat acacggtgtt ccagaagtag cttcttcgaa
     1981 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
     2041 ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg cgaagttaac
     2101 aatctttctt tgatcaattg aaaattctgc ctaagtagtg atagatcctg tgtgactttc
     2161 aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg gtctgcttct
     2221 tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata cttgacccat
     2281 tgattgacta attctagtag caacagacgg tattcttcat ccactgtact caactcaata
     2341 atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc agcgaaaatc
     2401 cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa gatgtcttcg
     2461 aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca aacttctttt
     2521 tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc tagaatatct
     2581 cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc ttccaagatc
     2641 ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta tattatacag
     2701 taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat tatctgacac
     2761 agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa gaactgcgag
     2821 gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt gtcatcactt
     2881 gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac
     2941 caattgcatc tgtcgctacc acaacattga atccactctc taccaattca cgcatatggg
     3001 aatcgctaca caagtttccg ttcattccac caagaattac tgtgtcgatt ccattttttc
     3061 tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga gagctgatga
     3121 tcgtattttc atccaaataa ggtttcaatt cttcgataaa atcggctcct gaaccttctt
     3181 ccactgattc atattgactt tttctagcaa acatctgctg ctcaagcatc atatgttctc
     3241 ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga gaaataaaga
     3301 gtttgtatcc tttttctttt gcttgtttta ccatttcaat catattttcg atcgtattgt
     3361 atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc aagaactcga
     3421 cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg gattctccat
     3481 tcgttattta ttttaataca tcatttagag cttcactcat tgtcggatga gaatagattt
     3541 gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc aagttgatga
     3601 cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt tcagggtcga
     3661 ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg acaccgcttg
     3721 tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt tctgttaaac
     3781 ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc cggttattcg
     3841 ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca tctagagaga
     3901 tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg
     3961 tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca tctgtatttt
     4021 ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact tttgacgctt
     4081 ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat tcttggtctg
     4141 ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc atgtcttcta
     4201 aaatcatttg cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa
     4261 cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca atatatccag
     4321 ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc gtagaatcaa
     4381 tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca gaaccagtat
     4441 tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc acttcgattt
     4501 gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc tcatcgctga
     4561 tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga gcaacagcct
     4621 ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga atacagccga
     4681 tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa accgattcac
     4741 cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg atcacaatat
     4801 tttgatattt ttccatcatt tttccctcgc ttctttttta cctaccgatt ggtacaaaaa
     4861 tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca aaaaaaacca
     4921 gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt cttctaaaca
     4981 atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca gaattcatgc
     5041 gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg cacttctaat
     5101 actacaccga tctgatagcc gccatcatct aaattataaa gagagacacg tgcttttact
     5161 gtggactctg ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc attgaaacaa
     5221 gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg gattcctgga
     5281 ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt gcgcccgcca
     5341 tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc gattgaccat
     5401 ttgctacttg gctataatta tagtgtatga caagtaaggc gctttctaca aaaattctgc
     5461 ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa aaatactact
     5521 cgtgaacaac agcgtcatat ttgttccaaa ataaatagca atcttttcta cataatcttt
     5581 tatatgattg attttaagat acatatatac cagaaagttg accccataaa ccaatactgt
     5641 taaaaaaata accatattcg ttaccatttt ttcctccttt tgctggtctc cctaccagca
     5701 ctcgatttaa ccataccact ttttttgccg tccaagcaac tagcctttca aaaagactgg
     5761 tttattctgc tgttaatgat tcaagatcag aaaaatgtcc gaataatttt gctaatttta
     5821 ctaacgccaa cttgttcttt ccgaattcat cattttctat ctgtaatact agtaacggct
     5881 catgtaagct taaacggatc aaaaaccagc cgtttccgta tggctcttct agatcatatc
     5941 gaataccttc ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct
     6001 tcatttcttc taatatcttg tttccttgtt cgcgtggttc atcgctgaac aattttaatc
     6061 ggatttcttg tacttctacg ggctgtttca actctttgat ttgttcatct aatgtgatac
     6121 cttttctttt taattctggc aaaagcatca ggatctttgc gatgacatat gcaccatcat
     6181 ccagaaaata gttttccttg aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat
     6241 ctttcccaag acggttaagt tctaatgctt tattgattac atttcgatag ccggaaatat
     6301 aacgaacttg gatgcctcct ttttcttcaa tgaatctttg tagatgagaa gaagtagggg
     6361 aattcgtcac gattcttgtt cctggatgct cttttaatac gattgtactt aatactgcga
     6421 tcaaattatt tcgattgatc actgttccag aagcactgac aactgcagaa cgatccacat
     6481 ctgtatcgaa aatgatccca agatctgctt catttgctaa aacagcctgc ttgatacttt
     6541 ccatcgcttc tttattatca ggatttggga tatgattagg aaatcggcca tctggttcca
     6601 aaaattggga acctgttgaa tccgcaccta agctattcaa tactttttca gcaaagaatc
     6661 caccagcacc atttccggca tcaacaatga ttttccaacc atttagtggt ttgctttcta
     6721 gtgttccaat ccccttttta atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt
     6781 ctgctttttc tacgcttcca gtttgttcgc tttttatttt ttgctgctgt gataggataa
     6841 aagcaatatc ttctttttct gctcctcctt ctgaggtaaa gaattttatt ccattataat
     6901 aatagggaag atggctagca gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt
     6961 gagtggacat aaacatagca ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt
     7021 gttctttcag actttggatc aacgcctctt tcagtgctgg tccgcttatc ctactgtcat
     7081 gaccaatccc aatcgtccat tttttatttg gatcggttga tttcttcttt ttctttatcc
     7141 agtttgctag tccccagccg atctttttta ccatgtctgt cgtcaagttt acttcatatc
     7201 cttcaactgg taatgcgatt cctcgaatat ccgatccatt ttgcagcgct ttcatatctt
     7261 tcatataaaa gtcctcctca tttattccct cattttttcc agcttttctg caaatcgctg
     7321 tgcttcttct tctgagcggc aaataataat gaatgtatct acaccggcca tcgttccgac
     7381 aacttcaggg taagccactt catctagcca attcgttacc acatcggcac catttcgttc
     7441 agtcaatacg ataaccatga actgaacaga ctggatacgc aaaacctcgc gtctaattgc
     7501 tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg ttaaataatg tatagcgtac
     7561 tattttgttt tcattttgac tttttactaa cttcatttct cgaatgtctc gagaaatcgt
     7621 tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca agcagctctt cctgtgtccc
     7681 aataggttgt tctgtaatga tctggcggat cagtgcttgt cgctgtttct ttttcatatt
     7741 agacacctct caatgttgat tatagcaaaa gataagtaat atttcttcat attttggtat
     7801 tttttacttt tattttttgt tttcattcag gttgctaacc tttttctatt ttaacacgca
     7861 aaatcgtctt cattttttca ggtgctgatt ttcttggatc gctgatgtaa atctctttgt
     7921 gttctttaga agctctttca tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg
     7981 ttgctggttc ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca
     8041 tctttttgaa tactacttgt tctttcaagt cttccggaag tttgctagaa cgctgaatgg
     8101 cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg gatcatcaat tgataagaaa
     8161 aatgttcttt tttcatcact ggttcatgca aatacttttc ttgcagtccc cattgtcctt
     8221 ccagtggata gacagtatac atctgataat caggaatcag ccaatttttt ttaggcgaca
     8281 tgcgtattgc atagctgact gcatatagac aggctactct tcgtttaaag tcttcctgat
     8341 tcgggtcgcc gatccctctg atgctaaaga atgatttttt aggaacctct agtatttgcg
     8401 ggcttttttt catactatag ctcttgtctt tacgccattc aattttttcc atttttcttc
     8461 ctccgcgaaa ttccatttag ttatttatta tcatacctct ttttgagtga cttggctaat
     8521 cgtgttgaca ctatttttca gtttgtgcta gaataagtga agtaaataaa tcgtacagat
     8581 aacacgtttc ggcgtgtcga gagagaggcg gtttctattt ttataataga aatcactctc
     8641 tttttttacg aaatttttta gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag
     8701 tacctgcagt aattgcaaca gggatgatgt cttttgcggg cgtattgatc gagacggcca
     8761 tgaatgtaac tttcccaact cttatcagac agtttgattt aacgactgca caagtccaat
     8821 gggtaacaac gatttatctt ttgatgattt cgatcattgt tccgctgtct acttatttga
     8881 atcgcaattt tagtctcaaa aaactctttt tagcttcaaa tctgcttttc ttggcaggta
     8941 taatgatcga tttcttctct cctaccttca tggtcctatt gtttggtcga ttgctccaag
     9001 gtgcagcgac aggtatcgct cttccgttga tgttccatat tatcttgaca tttgcccccc
     9061 tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac aacggccatt gctccagcaa
     9121 ttggccccac ttatggaggc attatgactt cacattttac atggaatcat atcttcctgt
     9181 ttcttgtgcc cgttttgttt ctttctttga ttatcggctg gtttgctatt cccgagatgg
     9241 cagtacaaaa aagtggttat ttggattgga taagcgtttt aggaatttgt cttttgttta
     9301 gtggaagtct aacatttttg agtacgttgg gaacagtttc cagctggatt gctttaatca
     9361 tcggcggtat tggctggttc cttttttact atcgaagtaa aaaagtggag catccactga
     9421 tccgactgtc tattctttca aataaaacat tccgcttgtt tttgattggc ttcttagttt
     9481 atcagttttt attacttggt gtatcttttg tcttgccgaa tttccttcag atcgttcaag
     9541 gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc
     9601 tttctccttt ttccgggaaa atgctggatc aatatggtcc gaagaagcca atttttgctg
     9661 ggctgattct ttctttgatt ggttggctag cattattcct actcatagga cacgcttcag
     9721 tgggtgtatt gattgcttgt catgtgtttt atatgatcgg tatcggatta tcttacagca
     9781 atatgatgac caccggaatg aatgcactga atgaagaact acaaggagac gggaacgcaa
     9841 tgttcaatac cttgcagcag ttttctggcg ctgtggctac ttcattagta gcggtcatta
     9901 tcaattacgt ccagcatcat acttcccata actatgaagt gtctactaca ttaggatcaa
     9961 aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt
    10021 atctgttctt tgctaaaaaa gcgtagattc catatacggc aggtgttgca tctgccgttt
    10081 ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa ttgccattat
    10141 cggtggcggg atcattggta tgaccctagc cagttactta gatacatcaa aattcaatgt
    10201 tgctttattt gatgaaggga caggacaggc aactaaagct agcgctggga ttatctcccc
    10261 ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt
    10321 tttccctaaa cttataaaag atttccaact tgatcaaacg atttacgcac aatcagggac
    10381 acttttgctt cgacctgaaa aagatttagc tgaattggct gttcttgccg agaaacgaaa
    10441 agaagaagcg ccagaaattg gaaacatcga atggctttca cctgaacaga catctcaagc
    10501 acttccgtta ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc gtctggatgg
    10561 aaaagcatat ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg agcagatcca
    10621 ggcaaaagtc actttgcata agaagagcga ttcttggctc atcaagggac cgtttggaga
    10681 atacacagct gatcgtgtaa ttttgacacc tggcccggca cttaagcctt tattacaaga
    10741 attaggctat gctgcagata tacgtccgca aaaagggcag ctcttagcat ttcagacatc
    10801 ttataagatg agtcaagact ggcctgttgc tatgcttgac ggagaagctg atttcattcc
    10861 tttcatggat ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag gctgggattt
    10921 ggctcctact agcgaagctt ataagggatt gactaagagt acagaacagt tcttgactga
    10981 tccagatcag ataaaaaact ggccatacga gttccgtgta ggtacccgtg cttatacgtc
    11041 agatttttcc ccatttttcg gcgtactgcc agatgatcca agcttactgg ctgctagcgg
    11101 tctaggctct tctggcttga ccacaggtcc ttatatcggc tatctattag ccaacttctt
    11161 aaatactggt cattggccta cggaagatta tcaaaaggac atctccacct atattacgaa
    11221 aaatccatca tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga
    11281 ttacttcttg aaaaagctgt tattttagtt caatttctga atcaaattct ccaaactttc
    11341 ttttgcttca tctggcaaga gatcttttcc tatctgagct tcaatgaatc gtaaacgaat
    11401 atcaagtcgt tgtttcacca gatttttata ggacgtatcc tcaaaagaaa caggaaaatc
    11461 tggtataggt aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg
    11521 ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa
    11581 atatccagta ttcaggacat agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc
    11641 ttgatagtct tccctaagag gatagacacg gaaaggatta gcaaatggtt caatgactag
    11701 ctcatctagc tgtctttttg tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt
    11761 tgcaccaaaa gacgctgcta aaactgggtc attgatcttg ataattggcg gaagcgaatc
    11821 atctttcatg atccaaaaaa cagcatcgat cttttcgcta agatgatcga ctcggtcggg
    11881 tgtcgcataa cgtgatttga ctgtccgccc atttccgttt cgtatatctc ctgttactag
    11941 gactttttct cctttacttg tacatgtcac gcccacattc tgacaagtaa ggaaagaaga
    12001 aatggccgga tcatcagacg gatagtcttg tgttttatca aagtaagaag gttccaaggc
    12061 agtagcaaaa ccattttttc gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga
    12121 gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa
    12181 tacagccatc gtataggtct ggtcattact agcatattgt ttcatcccac cgtggcaggc
    12241 aataaagcca ttgcgatttc ccaatgccca agcaagcgtc aaagtcgctt tttttaattc
    12301 tccaaagtaa cgtaatccta gcacagccgc tgtattatgg agagcatcaa acaaaatgag
    12361 tccatttgga aatgtttcat gtgtccattc gggatcacaa aagatataaa gatctccctc
    12421 atcaaaggaa acagactttt gataagtctc taagatcttc gaattcgctg gctgaaaatt
    12481 cagcaaataa gaatacaaat tgttctcaaa tccttctgga atcagcaaat gactttgcac
    12541 agaaaattct tcagataggc caacgattac ttttgtatgg taaaactggc gcttgcgact
    12601 ttgatacaca gcttctctta aaattttctc gtatttgctt tgttccttgt ctaagtcctc
    12661 aaagatccgc cgagcacttg ctgttcttcc cacaatgctt ccatcattga atactagtgt
    12721 cctaacatca gctggtaaat ctagttcttc agcatgtgag attgttaaat ctgtgatgat
    12781 cgttcctgga ctcccttttg ctagttgata agctgtcttc ctatcaaaaa cttcatgaac
    12841 attattaccg taaaatgtcg tttctacaat cgatcgaata ctggataaca gtggatttcc
    12901 acttgtgatt tcgtttaatt cgagactctc ttttgtactc atggctcctc ctcattccct
    12961 aaacaaaaaa acgttttctt tattatacat tattcgagtt tttatttgta ctgctgcgct
    13021 gtgggaagca aacgagttat ttttcacaca aaaaaaccga gaacttgaat aaacaagttt
    13081 ctcggtttca tcattttcct tttatcggct tagtaccagc cgtttgctaa ccagaatgtt
    13141 ttagcagcat cccatgaacc ataacgagat gttacatatt gttctgctac acgttcttga
    13201 ttggcagctg aatagtcgcc gtttaagtat gaagcatcta attgataacg gccgatgtaa
    13261 cgtccgttag tagctgtgta tgaaccgcct gattcttttt gtgcgatcca ttcttttgca
    13321 gagcttgaag ttggcgctgt tgcagcagct ggggcagcag gagctacttc aactgtttca
    13381 acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct
    13441 gttgttttgt tttctgttgt tgctgaagaa tcatttgaag ttgggattgt taattgttca
    13501 ccaacataaa tgcggttgat gtttgcgatc gaattttttt cagcaattgc atcgatcatt
    13561 gagttgtctc ctgcaaattt ttgtgagatc ttagataatg aatcacctga ttttactgta
    13621 taaacttcat cagcgtgagc atttgtagtt cctacaaaca ttgcagtagc accagcagca
    13681 aaagttgtac ctaaaacaat tttcttaaat gaattcatat taaactcctt tttttctccg
    13741 ttttttattc tttcgacgct attaacttta
//
LOCUS       DBKOIJ010000064        13609 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000064 DBKOIJ010000000
VERSION     DBKOIJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.066"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..611)
                     /locus_tag="ACV5NJ_001513"
     CDS             complement(<1..611)
                     /locus_tag="ACV5NJ_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HHY6485610.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYL"
     gene            complement(859..1695)
                     /locus_tag="ACV5NJ_001514"
     CDS             complement(859..1695)
                     /locus_tag="ACV5NJ_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HHY6485611.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1706..2635)
                     /locus_tag="ACV5NJ_001515"
     CDS             complement(1706..2635)
                     /locus_tag="ACV5NJ_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HHY6485612.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2827..3180
                     /locus_tag="ACV5NJ_001516"
     CDS             2827..3180
                     /locus_tag="ACV5NJ_001516"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HHY6485613.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3254..3910)
                     /locus_tag="ACV5NJ_001517"
     CDS             complement(3254..3910)
                     /locus_tag="ACV5NJ_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HHY6485614.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4136..6070
                     /locus_tag="ACV5NJ_001518"
     CDS             4136..6070
                     /locus_tag="ACV5NJ_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HHY6485615.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6156..7379)
                     /locus_tag="ACV5NJ_001519"
     CDS             complement(6156..7379)
                     /locus_tag="ACV5NJ_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HHY6485616.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7373..9244)
                     /locus_tag="ACV5NJ_001520"
     CDS             complement(7373..9244)
                     /locus_tag="ACV5NJ_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HHY6485617.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9265..10383)
                     /locus_tag="ACV5NJ_001521"
     CDS             complement(9265..10383)
                     /locus_tag="ACV5NJ_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HHY6485618.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10466..11299)
                     /locus_tag="ACV5NJ_001522"
     CDS             complement(10466..11299)
                     /locus_tag="ACV5NJ_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485619.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11292..12209)
                     /locus_tag="ACV5NJ_001523"
     CDS             complement(11292..12209)
                     /locus_tag="ACV5NJ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485620.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12232..12411)
                     /locus_tag="ACV5NJ_001524"
     CDS             complement(12232..12411)
                     /locus_tag="ACV5NJ_001524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485621.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(12436..13560)
                     /locus_tag="ACV5NJ_001525"
     CDS             complement(12436..13560)
                     /locus_tag="ACV5NJ_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6485622.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4244 a         2722 c         2236 g         4407 t
ORIGIN      
        1 cataaatact catattcgag tgatttttta tattttttaa ttgtgccgtt tacgagaatc
       61 tcttcatctt tcaatactag gcctatttta tttttataac ctggcatgct cattagataa
      121 aggatttcat cgtaatggat atagtgatcg ctattaccat aacggacagt tgtccacctt
      181 tttttcgtag catgttgaac aggatagttt tctttttctt tcaataagta actttggatg
      241 ctttttttat ttttttcata agtgaattct tttatcaaat aaccactagg cgtaatcttt
      301 cgattaataa cagttattgc tttattcgga tgagacgtta gataaacaat tttacacttt
      361 tcattctttt ctctaagcat ttcgcctagt tcgataccgc tataataggc gttcaaatcg
      421 atgtctagga aaaaaatatc tgaggaatag atttcattat ttttaatgaa ttcaacaaat
      481 tgtataacgt tcaatccctg aacgatctca aatttccaag gacttaataa aattctggat
      541 tcttccaaaa tttctactag cattttttca aaaaccaaac tatcatccaa aataaatata
      601 cgcgttacca taaaaagtac ctccactcga tattttacta tcaaaatcat ttcatacaac
      661 ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt ccaacacctt
      721 tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa
      781 tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt ctgagctatg
      841 tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg gagcagagac
      901 tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt gataatagtc
      961 tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta ctccttccgg
     1021 catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa tgccgaatcg
     1081 gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc cttcatagct
     1141 gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa cttctaaata
     1201 gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca actcattcat
     1261 ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta agtattgtgt
     1321 ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc ctttggcttc
     1381 tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta ctggcaaagc
     1441 tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt catgatcgtg
     1501 ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct gaactaaagc
     1561 caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga cattgatata
     1621 ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc cagataaaat
     1681 caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata acgctaaggg
     1741 caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc atcatcagta
     1801 gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt gctgtgccga
     1861 acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga gacatcgtaa
     1921 aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc gttagcataa
     1981 ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg atcaaataac
     2041 gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt tgatggatca
     2101 gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga tgatggtgat
     2161 tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga aaataggcaa
     2221 gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag cttgccatct
     2281 gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg atgatcggag
     2341 cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa
     2401 cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa
     2461 aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg gtcaaaaata
     2521 cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct tcaataacga
     2581 tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt
     2641 tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt caaaaaaaac
     2701 aagttgctct gtaaattttt acacaactct tattttttac caaagcactt caattgactt
     2761 tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat aaaagggaga
     2821 ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca acagtcatcc
     2881 gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga
     2941 ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc atttgaaaca
     3001 ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa
     3061 ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg ttatctacta
     3121 gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg aacaaaataa
     3181 tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc atttgttcaa
     3241 tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga
     3301 aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat acatctgacg
     3361 gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct tccgttaact
     3421 gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact tcgatttgtt
     3481 ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg tctacgatgc
     3541 gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta ttactttgat
     3601 ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc gcaacatttg
     3661 tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct acatcataac
     3721 catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc gttgcactat
     3781 ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca gcattattta
     3841 aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt ggaataatat
     3901 gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt ctccatcata
     3961 gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt ttattcttta
     4021 ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg tagactacta
     4081 attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg attccatgat
     4141 attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat
     4201 acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa
     4261 atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa agatcgctaa
     4321 aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt ccaatgaaac
     4381 agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag agcggatgcg
     4441 cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata gttcttattt
     4501 aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg gttatgaaaa
     4561 tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg atcagaaaat
     4621 cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc ttttgcaaca
     4681 ccctgacatt ttgattctag acgaaccaac gaaccattta gatatcgaga cactttcctg
     4741 gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc atgaccgtta
     4801 ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa tgcgccatta
     4861 caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa gtgaatggaa
     4921 ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg ctcggaacct
     4981 tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag aaaaaatgga
     5041 ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca gtatagaaaa
     5101 gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg ataatgtcac
     5161 cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc
     5221 gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc cttttatccg
     5281 tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag gtcaagctga
     5341 tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta ccactccaga
     5401 aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg tagaaaaacc
     5461 aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa
     5521 caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg acaacaaaga
     5581 agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt cccatgaccg
     5641 atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt
     5701 ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag aaatcgccga
     5761 attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta
     5821 ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga
     5881 agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc
     5941 agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag
     6001 acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata
     6061 tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt tttctttttt
     6121 tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga ccaattacta
     6181 gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg tgtatttacc
     6241 ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc ttcttccgtt
     6301 gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg gtataagttc
     6361 ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca aaccatgatc
     6421 accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa atcccaataa
     6481 caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt gcttaaatca
     6541 aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat cccccatgct
     6601 tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc atatcttggt
     6661 tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc tcttactaaa
     6721 taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg atttccagtt
     6781 tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat cattctacct
     6841 tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa
     6901 cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg atttcccgtt
     6961 attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg atgattagtg
     7021 ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc tgtttgttga
     7081 aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca aaatccttct
     7141 gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc gcttgggaat
     7201 tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg acaacgctcg
     7261 atctcattat tccattcttt ttccattaat tctttataac attcatttat ccatttttct
     7321 ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc ctttagcata
     7381 attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc ccatcaaaat
     7441 caattttggg acgattgttc catacttcta aactataatc ccgatcttga tgagcaacca
     7501 ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca ggaataaaag
     7561 tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg gattttcgtt
     7621 taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc accctatagt
     7681 tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca atagcaaacc
     7741 ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga atctgccagc
     7801 cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc catgtagagg
     7861 caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg aactgtccat
     7921 aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc
     7981 ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt atcgaaaaac
     8041 caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta tggaaatttg
     8101 gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat
     8161 tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct ttagccgtcc
     8221 aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc cataatggag
     8281 ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa ccaatattca
     8341 ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc cgattgacta
     8401 accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata gctgaccaaa
     8461 agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt gcaccatctt
     8521 ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta tatatctggc
     8581 taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga aatgcaaaag
     8641 gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt tgaagataac
     8701 aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga taaccaaatt
     8761 tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct gcacattcat
     8821 attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta tctaattttt
     8881 tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt tctaccatac
     8941 gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct gttccagctt
     9001 ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg
     9061 ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca tatcctaccc
     9121 cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg agtggttgat
     9181 ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt actggatgtt
     9241 tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt ctaatcgaag
     9301 taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag tcagttctct
     9361 atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc aatttgcata
     9421 gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt ttgatcgtac
     9481 agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc cagcactggt
     9541 atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag ttttttcgat
     9601 tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg gattttttgt
     9661 gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc tccaacaaga
     9721 attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa
     9781 ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg ttgtatctgc
     9841 atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag cccaatacag
     9901 taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt cattccatgc
     9961 tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg catgtaaccc
    10021 tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg atggtaagaa
    10081 taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt ctaataattg
    10141 ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca
    10201 taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct ttattacgtc
    10261 tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt ctaattccgc
    10321 atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg tctgttcatt
    10381 catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct agaacgaact
    10441 agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca ttctccttaa
    10501 atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt gctaaaatta
    10561 gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata tttttgataa
    10621 ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat agatattgat
    10681 tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat aaaatcggta
    10741 cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca gcctccatca
    10801 gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt gtactggcaa
    10861 tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc ataatgtaaa
    10921 ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact gtattgaaca
    10981 acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt gtagaactgg
    11041 ctgtagctga aactgtactg attacagcaa taacaatcga attccaaata atcaagggat
    11101 aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca aacttgaaat
    11161 tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc atatataaat
    11221 atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa attctgcttt
    11281 gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc cgataatata
    11341 acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc aaccatttca
    11401 aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg tccattggaa
    11461 gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt ggctaaaact
    11521 gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat
    11581 ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc ttgaagacca
    11641 gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat gatcattgat
    11701 attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag cgcattcaaa
    11761 aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc tacagccacc
    11821 gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc ttttaactgt
    11881 gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg aattgatcct
    11941 gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg gaagatagct
    12001 agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa actaaaagca
    12061 aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa aggtgtgacc
    12121 aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct gttctttatt
    12181 gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt tttaatcacc
    12241 agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat atgaattttc
    12301 aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg tagcatctga
    12361 ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca atagttcata
    12421 gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt gataggccaa
    12481 tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt catctgtcag
    12541 cataaatgta agaaattgtt gtgccacttg tggatttttg gcatctttag ggacataaaa
    12601 acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag gaatcagtgg
    12661 agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg tagcatacca
    12721 gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt gtgcaccgta
    12781 gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat gtttcgcttc
    12841 tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct gctgggctag
    12901 catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa aactaccagt
    12961 tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga tttcttcaaa
    13021 atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg gggtaaaatc
    13081 actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat tcaatttttg
    13141 atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac tataaaaagc
    13201 atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga taatatccgg
    13261 catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg ttcccgaagc
    13321 tacattcaca atttttaagt caatatttgc tccagttttg cggttataga tttttgcagc
    13381 attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg tagtggcttt
    13441 ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc cacatgcact
    13501 taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc tttttttcat
    13561 gtctatccct tctttctttt caagttctta tataaaacgc tttcactta
//
LOCUS       DBKOIJ010000065        13309 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000065 DBKOIJ010000000
VERSION     DBKOIJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.067"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            192..959
                     /locus_tag="ACV5NJ_001526"
     CDS             192..959
                     /locus_tag="ACV5NJ_001526"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485623.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            949..2991
                     /locus_tag="ACV5NJ_001527"
     CDS             949..2991
                     /locus_tag="ACV5NJ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485624.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            3272..3421
                     /locus_tag="ACV5NJ_001528"
     CDS             3272..3421
                     /locus_tag="ACV5NJ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HHY6485625.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            3432..4946
                     /gene="dltA"
                     /locus_tag="ACV5NJ_001529"
     CDS             3432..4946
                     /gene="dltA"
                     /locus_tag="ACV5NJ_001529"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HHY6485626.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            4943..6148
                     /gene="dltB"
                     /locus_tag="ACV5NJ_001530"
     CDS             4943..6148
                     /gene="dltB"
                     /locus_tag="ACV5NJ_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HHY6485627.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            6193..6426
                     /gene="dltC"
                     /locus_tag="ACV5NJ_001531"
     CDS             6193..6426
                     /gene="dltC"
                     /locus_tag="ACV5NJ_001531"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HHY6485628.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            6429..7697
                     /gene="dltD"
                     /locus_tag="ACV5NJ_001532"
     CDS             6429..7697
                     /gene="dltD"
                     /locus_tag="ACV5NJ_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HHY6485629.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            7838..8920
                     /locus_tag="ACV5NJ_001533"
     CDS             7838..8920
                     /locus_tag="ACV5NJ_001533"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HHY6485630.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            8920..9723
                     /locus_tag="ACV5NJ_001534"
     CDS             8920..9723
                     /locus_tag="ACV5NJ_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HHY6485631.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            9793..10533
                     /locus_tag="ACV5NJ_001535"
     CDS             9793..10533
                     /locus_tag="ACV5NJ_001535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HHY6485632.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            10544..11548
                     /locus_tag="ACV5NJ_001536"
     CDS             10544..11548
                     /locus_tag="ACV5NJ_001536"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HHY6485633.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(11593..>13309)
                     /locus_tag="ACV5NJ_001537"
     CDS             complement(11593..>13309)
                     /locus_tag="ACV5NJ_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485634.1"
                     /translation="PADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFI
                     METTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYI
                     GKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFY
                     TIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSA
                     FIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTL
                     VTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIIL
                     IVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEME
                     NLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQIT
                     FQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCE
                     HSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRN
                     YPNPV"
BASE COUNT         4263 a         2186 c         2697 g         4163 t
ORIGIN      
        1 attgtgaaaa aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg
       61 ctgaataaaa taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata
      121 agagcaaatt attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag
      181 gaggagaaga attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca
      241 aactttctcc cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg
      301 ttgggattat gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga
      361 tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa
      421 acagtgagct cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt
      481 tgatcgatac cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga
      541 ctaaagagga aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac
      601 tgctcaaacg gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta
      661 gagcacttgt tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact
      721 cgaagtcggc tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa
      781 cgatactgtt agtcacccat gatccatata cagcaagcta ctgcgataga attttattta
      841 ttaaagatgg tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg
      901 agaaagtaat agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa
      961 ctagccgtta aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg
     1021 actttatccg taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc
     1081 agaagagcaa gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt
     1141 actgttgttt tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa
     1201 aaggaaatag gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat
     1261 gtgttagaaa tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt
     1321 attttttcca aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt
     1381 ccatttttta tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt
     1441 ttagcagtat ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt
     1501 ggagaaaaag aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc
     1561 gcagtcatcg ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa
     1621 atcacttcat ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt
     1681 ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta
     1741 tttttgatca tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg
     1801 ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt
     1861 ctcggaattt ctttaatgac aattggcggc acggctagta tctctactat tttatcctac
     1921 caggagaaag tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc
     1981 aagccgattc tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa
     2041 gtcattggga gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg
     2101 atcacggaaa cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct
     2161 ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt
     2221 tcattatatg gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg
     2281 cttccaagtg tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat
     2341 gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca
     2401 ggtggaaata atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc
     2461 aatgctgttt attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca
     2521 aagaaacttc cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg
     2581 aatttctctt ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt
     2641 tttgtcacac tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc
     2701 cggcaattag cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg
     2761 attccacaaa gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca
     2821 cccatgatat tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata
     2881 aaaactgccg attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt
     2941 ttgctttatg tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa
     3001 taagacaaga taataaatag aactaaattt tgaataccat ttatttggaa taagaggttg
     3061 tgacacgatt tttgtcacaa cctctttatt ttttatattt tttaaaagta cgagaaatta
     3121 taaagaaaaa agcagtgatt tgatgtgcta tttttataaa agctatgtga caaaactgtt
     3181 agtaaaaaaa cggtttctgt aagctagaca catgtttctt tttgtcaaaa ttagttacac
     3241 tttgtgtgag gttatatgag gagggaatcc aatgaacaaa tggcgttcta atccaaaatt
     3301 gaattattgg ggaaaattca tcattcgaac aattttttat agtgcagtac tgctgttcct
     3361 gatttatctg tatcactata aaaatattca aggaggaact tttatctata acgaatttta
     3421 ggaggaagag tatggaaatc aaaacgatta ttgaagcaat cgatgattgg ggaataaatg
     3481 aacctgatcg agttgcttat caggcggatg agacgcatac ttttggtgaa ttgaaacaag
     3541 catcagacgc acttgcttat tatttagaaa aaaaggtaga aggcgatgga ccaattgtcg
     3601 tttttggcaa tttggagttt gaaatgattg tctcatttct aggggttgtc aaatctggcc
     3661 atgcctattt gccgattgaa gagcatacac ccaaggagcg tatcctatcg atctttcggg
     3721 tggctaaacc atctatggtc atcagtatcg gggactggat agaagatatt ccttcggttc
     3781 ctgtcataac aaaaaaagaa tttcaagaaa tcaagaagat accagtagga ttttatccca
     3841 gaaatagcgt aaaaggtaca gaaaattttt atattatttt tacttctggt actactggag
     3901 aaccaaaagg cgtacaaatc agtcatgata atctagtcag ctttgttcaa tggacgatgg
     3961 aagactttgg gatcaagcct ggtatgcatt tcttagccca agcgccattt tcttttgatt
     4021 tatcggtatt ttctatttat cctgcattag tttctggcgg aatgctgaag ccgttaaata
     4081 aagcagttgt tcaagacttt cgtcaattat tcgcgacatt gccagccttg aagttgaatg
     4141 tgtgggtttc tactccatca tttatggata tttgcttgat ggaacctaca ttcaatggag
     4201 aaaatgtgcc ggaattggac atgtttttat tttgcggaga agaattgacg aaaaaaactg
     4261 cagaaagtct acttgatcga ttcccaaatg cacggatata taatacttac ggtccaacag
     4321 aagcaactgt agctatttca agtattcaga ttgaccagca tgtactagat agttatgacc
     4381 gactaccgat tggctacgta aaaaaagaca cgaaagtttc tattgtccaa gaaggtaaac
     4441 ctgtttcaaa aggagaaacg ggtgaaatca ttattgcagg accaagcgta tcaaaaggtt
     4501 acctgaacaa tcccgaaaag acagctgctg cgttcttttg tcaaaatgac ttagcaagtt
     4561 accatacagg ggatgcaggt agactagatg aatatgggct tttattttat gaaggacgta
     4621 tggatcagca ggtcaaatta catggatacc ggattgaact cggggatatc gaacattatc
     4681 tgctccaaga caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa ggaacaaaag
     4741 tacagcagct ggttgctttt gtcgtattag aaaacaaaac gaaacagccg gattttcagt
     4801 tgactaaatc tatcaaagaa caattactag aagttgtaat ggattatatg attcctcaga
     4861 agttccatta tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat cgaaaaaaat
     4921 taacagctga ggtgaatcct gaatgatgtt acctcatatg atcccttatg cagagccgtt
     4981 ttattttttt cttttagcca ttgcgcttgt accgatcgtt ctatctttat tgatatggga
     5041 aaaaagatta ccagtttatc aatcagcagt gacacttttc tttttatttg tcagctttgg
     5101 aggtaattat tggcaacagg gagcggcgct gattgggtat gttttgtggc agacgatact
     5161 tgtatggggc tattttgttt atcggcaaag gaaaaatcag acaggatggt tttttgccgc
     5221 cgttatttta gcgataattc cactgttttt aacgaaagtg actccttttt ttgaacatgg
     5281 aaaagcatcg ttacttggtt ttttaggaat ctcttattta acctttaaat ctgtacaaat
     5341 gatcatggaa ataagagatg gcatgatcaa aacatataag ccatttcgtt atatccagtt
     5401 tctgctgttc tttccaacaa tctcttctgg accaatcgat cgctatagga gatttgaaaa
     5461 agatcagctc catccgcctg ttcgtgataa atatgttgat cttttgggaa aaggcgtcca
     5521 aaatattttt gtcggttttt tatataaatt cattatcggt tattattttg gtcaagtact
     5581 gcttcctgta gtgggacgca ttgccttaca gcatggtggt atttcatggg cattagtagg
     5641 ttatatgtac gtgtacagta tgtatttgtt ttttgatttt gctggctata gcttatttgc
     5701 agtcggtaca agctacatga tgggatatca gacaccaatc aatttcaata aacctttttt
     5761 atcatggaat atcaaagaat tctggaatcg ttggcatatg acgctgtcct tttggttccg
     5821 tgattatgta tatatgcgat tgatgttctt catgatgaag aaaaaatttt ttaaaagcaa
     5881 gatcgtaatg tcaaatatcg gttattttgc tttgtttctt ctaatgggta tatggcatgg
     5941 attgacgtgg tactacatcg tatatggact gtatcatgct tgtctgatct gtctgacaga
     6001 tgcatggatc cgatttaaga agaagcataa agagcagttg ccttctaaca aatttacgca
     6061 tggcttagcc gtatttttga catttcaagc agtatgtttt agcttcctga ttttttcagg
     6121 atttttagat aaactttttt ttagctaacg aatagtgaca tattaattta tataacaaag
     6181 gagaaaagga aaatggaaga acaaatttta tcaatcttag aagaaataac aggaacagat
     6241 gaagtaagaa aaaatcgtga tattgatcta tttgatgaag ggttgcttga ttctttagca
     6301 tctgttcaat tattagtcga gctagacgga gagctggata tccaagtccc tgtatctgaa
     6361 ttcgagcgag aggattggtg cacaccgaat aaaatcattc aacaagcaac tgcattgaag
     6421 ggttgagtat gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta gctgttggct
     6481 tactgtttgt gttttttacc tcctcctatc aagtagatat ctctaatcca gaaacgatta
     6541 gacgtgcctc ttcttccatg tcagtgaacg tgttgaaagg agatgcgata aaaaattatg
     6601 cattatctga aaaacaatat attccttttt ttggctcaag tgaattaagc agaatcagtc
     6661 catttcatcc gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca tttttgctgg
     6721 gagcacccgg gacacaatcg ttaagccaat atatgatgat gcgttctgca ggagatgcta
     6781 tgaaaaacaa gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa aatggtgtaa
     6841 aaactgatta ctttaatacg tattattctg aacttcaaac atacgactgg ttgttttcaa
     6901 tgaagaaagt aacgcctgct gatcgctatt tagctagaag attattaact ttctcaaaag
     6961 taaaagagaa tgatacgttg acggcgattc tgcaaacgat caaaaaaggg aagctacctt
     7021 tacctgaatc tttgaatcag ctgcgcagcc aatggaacat gctgaagaga gaggatgaag
     7081 tattcagcaa tattgggttg aaagatcgac agcaaaaaat cgatcatgag tcaaaacgat
     7141 taccaaaaca ataccaagaa acagaattat ccattttagc aaatcaaatt ggagaacgag
     7201 aaacgacgaa taatccattc gggttgaaaa acgattttta tacgcatcgt atccgagccc
     7261 acgagccaga gctcaaacag tcacaaaaaa attgggatta tcgtttttcg ccagaatttt
     7321 ctgattttca gctggtcttg gatcaattag caaaaaacca taacgaagta ttgttcatca
     7381 tcccaccggt taacgagaaa tggagcgatt ataccggact atctcaagaa atgttacaag
     7441 gatttgccaa aaagatcaaa tttcaattaa atagccaagg attcaatcga atagctgatt
     7501 ttgtcaatca agcgggtacc aactatttta tggaagacac gatccatcta ggctggaaag
     7561 gctggttggc tgcagatcaa cagatcagac cgtttttaga agaaaatcat attactgcta
     7621 gtaagtatca tcttgacgat gcttttttta gcaaatcttg gcaacatcaa ataccagata
     7681 aactacaatt aaaataaaga aaacaggcga gtgttgctcg cttgttttct ttatttttga
     7741 atattctgaa taaaagataa aaataccaag tttgcctgaa caaaaatcat aagcaatggt
     7801 ataatcacaa tatgaatata gaaatggggg aaaacatttg gatacaaaag aagaacagct
     7861 acttattgat ttaacttctg caaaaggagt acctggaaat gaagaagagg taagagaagt
     7921 attcagagaa tatgccaaac cattcgctga tgatattttt tatgacggtt taggcagtgt
     7981 gattgccaaa catggtgctg gcggaggccc caaggtcttt atttctggac atatggacga
     8041 agttggtttt atggtaacaa aaataaccga aaaaggattc ttggaattcc aaactcttgg
     8101 aggttggtgg gggcaagtca tgcttgctca gcaagttgaa atcaaaacac gtgaagggaa
     8161 aatcgtccac ggtgtgattg gttccaaacc accacatgtt ttaacacctc aagtaagaaa
     8221 taaaccttat gaaataaagg atatgttcat tgatattggc gcatcaagcc aagaagaagc
     8281 caaagagtgg ggcatacgtc ctggagatat ggtcactcca tatattgaat acaaacgaat
     8341 gaatggcagt aaatatttac tggcaaaagc atgggataat cgtatcggga cagctgtttc
     8401 tctgcgagta ttagagaatc tatctaaaga agcacaccca aatgttttat tcgcaggaag
     8461 cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca agtacacacc ttgtaaatcc
     8521 cgatattgct tttgctttag atactggtac agcaggagac actccaggaa tgacaccaaa
     8581 agaagcagat tcgattttag gaaaaggccc acagattttg atttttgatg catctatgat
     8641 cccgcataaa aaattattga attttgttat tgatgtagca gaggaaatgg atattccatt
     8701 ccaatataca gtgatttctg gcggaggaac tgatgcagga caaatgcatt tgacaagaga
     8761 tggtgttccg tctgtagcca ttaccgttcc tgttcgttat ctgcattccc atacatcagt
     8821 gattcatgaa gatgattatt tgaatacagt gaaactagtc acagaattag tgaaacggct
     8881 tgatagtgaa acagttgaaa aaattcggag ttatgaataa tgaataatag aatacctatt
     8941 tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc aatggggaat caatacttgg
     9001 cttgttttct tatctataat atttacgagc gtcatccaga acttttttga cggtttcagt
     9061 ccttttccgg aagaaagcat gcagcagcac ttattgagtt ttctagtcga taatttactt
     9121 ttattcgcat tcaactatgc attgttttac attgcgctag tagttgtacg gggtggcaga
     9181 ccaaaaagta accttttatt ttcagttttc caaagaaagt attttggttc gatacttggc
     9241 atcaatttga tcaattcagt catcaactgg ctactcaaca gtttaatctt tctgcccatg
     9301 tttttaatca gcggagtgag tacttatatg cagctgcttt tcagttcaaa tgcggtacct
     9361 acgggtgtca tgccagaaga ttttcttgat atcagctttg ttttatcgat gatagggatg
     9421 atttttgttt cagcactcat catgagtatt gttggcggtt tattccaatt tgctgcatgg
     9481 acaaagttcg actatcctga tttgtcggtt attcaatgtt tgaaatatgg ctggttttta
     9541 ttgaaagata gaattggtac gtatattttg ttgcagctgt cttttatcgg ctggtatttg
     9601 ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt gaatgtgtca
     9661 gttgcagaat tttatgaaca agcacggatt gaaaaaggag gtccagacgc ctattttact
     9721 tagcatgtaa gcagtttatt catcaaaaaa ataacttttg tggtaggata aatctttgag
     9781 ggggaatgta cgatgaaatt aactgtttta ggatgcttag gagcgtatcc ttataaaaat
     9841 caagggacaa caggttattt gcttcaatct gacgggttca atttgttgat cgatgcaggt
     9901 agtgctacgc tgatcaaatt acaagaacac cttgatccac ttgatctaga tgctgtgatc
     9961 cttagccatt atcatcatga tcatattgct gaccttggtg tattacagta ttactggcaa
    10021 cttcatccta agagaaatcc ggagacattg ctttcaattt atggacacac aaaagattcc
    10081 attcactttg aagaattgac gttggaaaat gtctcaaaag gacatcctta ttttgaagct
    10141 gaagaattaa aaattggtcc ttttctgatt acttttatgg aaacaatcca tccagtagta
    10201 tgttacgcca tgcgtatcgt agaagaaaca acggggaaag tattcgtatt tacaggtgat
    10261 tcaggttatt tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt tctagcagat
    10321 acctatcttt ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa agaagctgga
    10381 gaacttgcga aatcagctaa cgtaaagaaa cttgtactta cacacctacc tcagtttggt
    10441 gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat tgatatagaa
    10501 cttgctgcag ttgacaaagt gtttaacatt taggaggaag aacatgattt ttgtaccaaa
    10561 tgaaaaccac gacccaagaa tcaatttagc aattgaacaa tatttgctgc aagaaatgcc
    10621 tttagatgaa cctattttgc tcttttatat caatgaacct tcaatcatca tcggcagaaa
    10681 ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca ttcatgttgt
    10741 ccgtcgctta agtggaggcg gagctgtcta ccatgatttt ggaaacctga actttagttt
    10801 tatcatgcct gatgatgggg attcattccg tgactttgcg aaagtaacac agccaatcat
    10861 ccaagcactt catgaattgg gtgttgctgg ggcggaattg aaaggccgaa atgatctagt
    10921 cattgatggc atgaaatttt ctggcaacgc gatgtatgct accaacggtc ggatgtttgc
    10981 tcatggaaca attatgttcg actctgatat caatgaagta gtcaatgcat taaaagtaaa
    11041 aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa cgaatatcaa
    11101 accatttctt ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc aagaaatctt
    11161 gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat tgactgatga
    11221 agattggaaa aaaatcaatc gaatctctga ggaatattat cgaaattggg attggaatta
    11281 cgggaagtct ccagatttca atttctctcg ccaaaaacgt ttttctatcg gttcgattga
    11341 agtccatttg aatgtctcag aaggaatgat caaagaagca aaaatctttg gcgatttctt
    11401 cggactagga gatatctctg acgtggaaaa aaaattggtt ggacagaaat atgataaagc
    11461 atctctatca aaaatagtca gccaaattga tgtgaagaaa tattttggtg cgatagaacc
    11521 agaagaattg cttaccttac tttattgaaa ataaaaaata gcggaatcca catatatgga
    11581 ttccgctatt ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc
    11641 aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat
    11701 catcattaag tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag
    11761 gtttgtatcg taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg
    11821 tatcgacaaa taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga
    11881 atgctctaaa gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc
    11941 gggaagatct gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg
    12001 cgctaagata agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt
    12061 tgcttcttct aatatctttt ccacatagtt tttagctttt gtttggtccg tatgcattaa
    12121 atcttgcaga ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat
    12181 atgctgtcga agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc
    12241 tacttgtctc cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat
    12301 aacgattaga atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc
    12361 taatgccgtt tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt
    12421 acaagctaca taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac
    12481 taaagtgctt ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg
    12541 agtaaaagtt gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat
    12601 tccgtaccat tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga
    12661 cgttaagata ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc
    12721 acttaaaata tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag
    12781 aataagtgag tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt
    12841 ccctatagcg ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata
    12901 aataaaacta atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata
    12961 gttctgataa ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa
    13021 atctatcaca gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta
    13081 taaaatcttt tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt
    13141 tgcttcgtcc aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac
    13201 ggtaggaatc gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata
    13261 gaaaacccac gaatctttta tttctttcat ttgattgctg tccgcagga
//
LOCUS       DBKOIJ010000066        13212 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000066 DBKOIJ010000000
VERSION     DBKOIJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.068"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            147..1016
                     /locus_tag="ACV5NJ_001538"
     CDS             147..1016
                     /locus_tag="ACV5NJ_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHY6485635.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACV5NJ_001539"
     CDS             1225..2250
                     /locus_tag="ACV5NJ_001539"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HHY6485636.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACV5NJ_001540"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACV5NJ_001540"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HHY6485637.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACV5NJ_001541"
     CDS             3333..5036
                     /locus_tag="ACV5NJ_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HHY6485638.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACV5NJ_001542"
     CDS             5038..7071
                     /locus_tag="ACV5NJ_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HHY6485639.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACV5NJ_001543"
     CDS             7082..7522
                     /locus_tag="ACV5NJ_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6485640.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACV5NJ_001544"
     CDS             complement(7722..8849)
                     /locus_tag="ACV5NJ_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485641.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACV5NJ_001545"
     CDS             complement(8852..9529)
                     /locus_tag="ACV5NJ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HHY6485642.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACV5NJ_001546"
     CDS             9793..11559
                     /locus_tag="ACV5NJ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HHY6485643.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACV5NJ_001547"
     CDS             11574..12011
                     /locus_tag="ACV5NJ_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6485644.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACV5NJ_001548"
     CDS             12024..13175
                     /locus_tag="ACV5NJ_001548"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HHY6485645.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aaacggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggggcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DBKOIJ010000067        13126 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000067 DBKOIJ010000000
VERSION     DBKOIJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.069"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..766)
                     /locus_tag="ACV5NJ_001549"
     CDS             complement(<1..766)
                     /locus_tag="ACV5NJ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHY6485646.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILE"
     gene            complement(763..1416)
                     /locus_tag="ACV5NJ_001550"
     CDS             complement(763..1416)
                     /locus_tag="ACV5NJ_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHY6485647.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1704..2224
                     /locus_tag="ACV5NJ_001551"
                     /pseudo
     CDS             1704..2224
                     /locus_tag="ACV5NJ_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2222..2941)
                     /locus_tag="ACV5NJ_001552"
                     /pseudo
     CDS             complement(2222..2941)
                     /locus_tag="ACV5NJ_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172851.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2892..3101)
                     /locus_tag="ACV5NJ_001553"
     CDS             complement(2892..3101)
                     /locus_tag="ACV5NJ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485648.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3378..4671
                     /locus_tag="ACV5NJ_001554"
                     /pseudo
     CDS             3378..4671
                     /locus_tag="ACV5NJ_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744739.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4758..5789)
                     /locus_tag="ACV5NJ_001555"
     CDS             complement(4758..5789)
                     /locus_tag="ACV5NJ_001555"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485649.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5796..6626)
                     /locus_tag="ACV5NJ_001556"
     CDS             complement(5796..6626)
                     /locus_tag="ACV5NJ_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485650.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6623..7438)
                     /locus_tag="ACV5NJ_001557"
     CDS             complement(6623..7438)
                     /locus_tag="ACV5NJ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485651.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7435..8259)
                     /locus_tag="ACV5NJ_001558"
     CDS             complement(7435..8259)
                     /locus_tag="ACV5NJ_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HHY6485652.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8249..9349)
                     /locus_tag="ACV5NJ_001559"
     CDS             complement(8249..9349)
                     /locus_tag="ACV5NJ_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6485653.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9527..10312
                     /locus_tag="ACV5NJ_001560"
     CDS             9527..10312
                     /locus_tag="ACV5NJ_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HHY6485654.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10345..10728
                     /locus_tag="ACV5NJ_001561"
     CDS             10345..10728
                     /locus_tag="ACV5NJ_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HHY6485655.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10804..10935
                     /locus_tag="ACV5NJ_001562"
     CDS             10804..10935
                     /locus_tag="ACV5NJ_001562"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHY6485656.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10904..11140
                     /locus_tag="ACV5NJ_001563"
     CDS             10904..11140
                     /locus_tag="ACV5NJ_001563"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHY6485657.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11152..11238
                     /locus_tag="ACV5NJ_001564"
                     /pseudo
     CDS             11152..11238
                     /locus_tag="ACV5NJ_001564"
                     /inference="COORDINATES: protein motif:HMM:NF012387.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11218..12189)
                     /locus_tag="ACV5NJ_001565"
     CDS             complement(11218..12189)
                     /locus_tag="ACV5NJ_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HHY6485658.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12841..>13126
                     /locus_tag="ACV5NJ_001566"
     CDS             12841..>13126
                     /locus_tag="ACV5NJ_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6485659.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3953 a         2897 c         2345 g         3931 t
ORIGIN      
        1 tttccagtat tattttaaga ctagcaagag gggttttaag ttcatgggat gcacctttaa
       61 aaaagtcata ttttaacttt tctaatttta aaatttcttt atttttaaat tccaaatcgt
      121 caattgttct taataaagta gaatacaaat cattaatttg ttgttttaat tctccaactt
      181 catcattaga gctaactttt aaactcattt ttttatcaag tttcatcatt ttatcagtaa
      241 caatttttat ttcttgtata ttatttttta ttgattttgc ataaattaaa gaaataatag
      301 cagaaaataa aattgatatt aataaagaat atggtaaaaa tttaaggctt aaatctttcg
      361 catctttttg catatcagct gtagaaacaa attgtagatt tgttttttta ccatcattaa
      421 gctttatttc tctttcttca attatcaaag aattactatc actttctaaa ttaacattta
      481 tattatcttt gatttgtatt tcattcttat tatttttttc ttttatgaat acttttattt
      541 cactactctt ggaataaaac tccaaagttt gttctatata ttttatttct tttccattca
      601 tattactaga aatttcattt gccatattat gaatcttttc ttttctcgtc tccaaatatg
      661 ttttaggaaa gataaaaaaa attaatgaat gaactaatat aattattatt ccaagaacag
      721 aaaatatttg tatgaacatt tttggaaata tctttaattt tttcattttc tctccaattt
      781 atatcctaca tttcttatag tagttataca gtctaattgt aatttttttc taagttcttt
      841 tatatataca tctattactc gatcataagg agtttcttcg ctatctttcc atacataatc
      901 aataatttgc attcttgtta acacttgtcc attattatcc aataaacatt ttaatacttc
      961 tagttctttt gcatttacat ctatttcttc accatttatt ttagctgtat agctattaaa
     1021 attaaccgat agatccttat attcaaactt ctctaaatgt ccataattct tcttaattaa
     1081 agaatctatt ctagctttta agaccggcaa tgaaaatggc ttttctacat atccgtctac
     1141 taaattagta aatgcatcaa ttttatattc ttcatcacta aatgcagtca agattagaat
     1201 tggtaaattg cttttctttc taatttcttt caacacttct aaaccattta taaaaggtat
     1261 ctgaatgtct aagataacca aatttatatc gctattaaat tttgacaagg cttctcttcc
     1321 atctttagct tgaataacag tatatccaaa ttctgaaaga tattcactta ttccctctct
     1381 tatcatatta tcatcttcaa cagttaatat tttcatatat acctccactt aagcctatac
     1441 actagatatt atacagcata ttatggctct ataatatcta gtgttggtga cgccttctgt
     1501 tgccaatact ttttcttttt tttaataaat gcaaacagac accttgaaat cctttagaaa
     1561 aaaggcttct ataatatttt gtgttgttgg aaaatcgaag gcgattttct actaacactt
     1621 tttcttcgtt tcaactcaaa aagagactca cttttggtaa aatttagtcg accaaaacat
     1681 caccctacca aaggagtcgc tctatggata atcatactag aaaattactc ggattaacag
     1741 ataaacattt attttttgat gaagaatggc tgattgaaaa agaatataaa ggcgttcaag
     1801 cgcagtttat taaagggaga ttggatgact caccttccca ttgtgaacac tgtggatcga
     1861 ttcgtattat ccgcaacggt tcctatacga cacgtactca gatgttgaaa gtcaaagaaa
     1921 agttgactat tttagaatta aaacgtaccc gttttctatg ctatgattgt gggcagacct
     1981 tttctgcgaa aacggactta gttgatgaac atcatcaact aacaaaggaa ctgaagcagg
     2041 cgattctcat agaactgtac gaaaatcaat cacgtaatct gaaaatcaga agaagtaccg
     2101 tcggctcaag cgatattgga aactcttact caaagattcc acgacattgg agccgctcaa
     2161 acggcattat catcgactgt ttaaacgacc aattagtcaa acagaaatcg tcgatgaacc
     2221 gttgtgcaca atggtgtcct gaacgtacag cgacaccttc catatccaga gcagtcgcca
     2281 catcacgggg atggatgcca tccaaattaa aagcaaccac gccagtatga tgttcaggat
     2341 cttgcggtcc gtaaatggtg ataccgccga tcgcttgtaa tttaggtaaa acgtaggcaa
     2401 ccagttcttg ctcataacga tgaatattct ccataccaat ctcattcaag taatcgattg
     2461 ctcctgcaat gttcggagta ccagcttcaa atttccatgg cagctctttc catgaactat
     2521 cgtaaagatt gacaaaatct atcatttcac cgccgaattc gactggctcc atttgttcta
     2581 acaattgacg tttgccatat aatacaccga ttcctgtcgg cccgcacatt ttatgcccgc
     2641 taaaggcgaa aaaatctgca tccaacgctt gtacatcaac tgtcatatgc ggtgctgctt
     2701 gggcaccgac tggactatcc tcaactacaa cagcctgttc aaccctcagc cgtttcaagc
     2761 acaacaaata accttctgga tctggtttgc cgacctgaag atcatcgatc gtcacaaagg
     2821 aatcaatttt ttccttaatc atcggatgtg cttcaaaaaa atagtctagc tcccgcagtt
     2881 cagaattgag actactccga ttttccagtg ctgctgatcc gctgctcaaa atgttcagca
     2941 tccactggtc tcccaaccgc ttgacttatc ccttcataaa aggacagttc tgctgccgtc
     3001 ccaacacctt gttgatagtc tgcaaaagga attttatagc cgaactgctc cagtaaatag
     3061 gcttcagtaa atagtgatga atactctcgt aaaacatcca ttcactgtca atcatcgtcc
     3121 catcaaaatc aaaaataagt gctttggtca tcttctttcc cccttaccca aaacaagcaa
     3181 aagctgtgtc ctgctctctc tacagtatca gcataaaaac tgccgtctga aaagtcaaag
     3241 gtgaaagaaa tgtggcgcta taatttctag gactgatagt aaatcattct atcagtcctt
     3301 ttgagcataa aaaaacaaaa catacagaag ctccgttaga attgaatcac cacaacacaa
     3361 tcaaaggagc gttctgtatg cctgatcttc atcttatcaa actattacta ggaatcaaag
     3421 atggaaacat tcaaatcact catgtcgaga agaaaaaaat acattcaatc aacagtatcg
     3481 ttgtttctgc cacgctcatt aaaacgattc agccctgtcc tcagtgtaaa caggtgaatc
     3541 aacatgggct gatcgttaag aacggaaaga aaaaatcgct ggtccttctc aataagtgtg
     3601 cgaatcaacg gacttatctt gctttggcaa agcaacggta cctctgtcgt ggctgtcaca
     3661 cctattttac ggcgaatacc tacattgttg aaaaaaactg ttttatcgcc aaacaagtac
     3721 attataaaat attggaggaa ttgactgaaa aacaagcaat gacgacgatt tctaaacatt
     3781 gcggcgtttc ttggtcaacc gtttcgcgaa cactcgcttc tctgctcccc atgacaaacg
     3841 tgaaaataaa ttggttgcct cgctgttcat tggtagatga atttcgttca ttgaaaaatc
     3901 aagtcggtcc ctattctttt tcttgtatgg atggcgatac tgggaaatta ctggatattc
     3961 ttccttcacg taagaagaaa gatctcattt cttattttat gcaatttgaa cgacgtgcac
     4021 gtttaaacgt aaaaatactc gtaacagata tgaacgcctc ttatgctttg ctgataaaag
     4081 agtgctttcc tcatgccaaa ctcgttgtgg atcgctttca catcgtcaag cacctcattc
     4141 gcagttttaa ggatatttga ctatgagtca tgaaaagctt cgaccgaaac gatccaatac
     4201 aagccaaaca ctatcgacaa gtgaaggctt tatctcgact tctgatcaaa cgacaggaca
     4261 cgcttgttta tgataaatgg acaaaatggc gtaattttgg ttgggcctat ttaaccgaaa
     4321 gtgaagtcgt agaacgtcta ctttctactt ctgatgaact acgaatagct tatgcttact
     4381 atcaagaaat tcttcaagct ttttatgaca aagaagcgga catcttcttt cagcttgtaa
     4441 aaacaatgcc caagtcggtt ccaagagaac tgcatcatat aaaaaaagcg tttataaact
     4501 atgaatccgg cattcgttta gccttagagt tgccttactc aaatggcaaa atagaaaatc
     4561 tgcataccca catcaaagca ttgaaacgaa tagcttacgg gttaggatga gaaccagaat
     4621 tttcttaatc aatcatctga tcacttatta aacataaaaa aacatctaaa gcagaaaatc
     4681 aattctacct tagatgttct tatatcactc tatcagtcct atttgacaaa gagccagaaa
     4741 tgtttgccga ctatacacta gctttgttgt gcaaaatgct gagtatcaat ttccacaggg
     4801 attgctagaa tatctttcag ctgaatgtat aaggcatcgc taattttctc ttcccgatcg
     4861 ttcaaccgat ccactaaaat ttctttgccc tccagcgttt gaaaaacata ccgttgaatg
     4921 ttgcctaaaa taaatgattc cttccataga accggaatct taaccccctg aggaaccggt
     4981 tcaaattgaa tcagctccgg tcttaaataa taaatgcagc gattgccctt agcggaaaac
     5041 tcgtgggtat actgattcag ctcataccat tcgaaacgat tatagttgcc gataaattcc
     5101 gcaacaaatc ggctctgagg attctgatac aattccttcg aggtggaaac ctgagccacc
     5161 cgcccagctt ccatcacaaa aatccgatca ctgattcgca tcgcctcttc ttggtcatga
     5221 gtcacaaaaa tcatcgtcat ccctagcgat tgctggatag cgcgcaaatc ccgctgcagc
     5281 tgttttctaa ttcgggcatc caaagcactt aatggctcat ccagcagcag gactttcggt
     5341 tcagtgacca atgagcgagc cagtgccacc cgttgtcgtt gaccgccaga aagaaactgg
     5401 acatttttat ctgcctgctc cgtcatatcc accaatgata gcatctcaaa aaccttttcc
     5461 tgaatcacct caggactcag cttctttacc ttcaaaccaa aggctacatt ttcaaaaaca
     5521 gtcatagttg gaaacaatgc atactgttga aaaacaaagc cgatattgcg atccttcgta
     5581 ggcttatcgg caatattctc attgtccagc acaatctcac cgctctttgg ttgttccaat
     5641 cccgtgatac agcgcagcaa cgtagacttt ccgcacccgc tgggacctag taaagtaacc
     5701 agctcaccct tttcaatctg aatttcatca atagctaaaa tctgtttttc atcaaaaaac
     5761 agattgagat catgtatttc taaaaacatt ttccgtcaca actcctttaa gggcttcttt
     5821 ttcgactgct tctcttccct cgtctgctta agggacagct gttgcttttg tcgataagtc
     5881 agcataaagg tagttaaagc aattcccacc ataaacagca aatacaaaac catcacagcg
     5941 ctagccaaat ggccgttttc gttcatccgc cgcagcatat agatgcgtac cgtctgaaat
     6001 cgaccgccaa tcaacaggtt cgtcagcatg tactccccaa aaaccgaaga aaaaatcatc
     6061 agagaaacta gggtcacacc gatacggatg tttggaaaca gcacctggat aataatcgta
     6121 gacatcgggg cgcccaaggt ttctgctgca tccactaatt ccttcagatt aattaagcgc
     6181 atctggttgt taagactaag atagacaatc ggcaacgccg taataaacaa tgcacctacc
     6241 aacactacaa acattgggat accagtttca gcatacgttc gaagcaaagc cgttaccaaa
     6301 atcactcctg gtaaagcata cggcagcagg agcagctttt ccagccaacg attcaaacga
     6361 gggaaataca aatgaatcca aataatggtg ggaatcatca acaacagaat cgtcaccaag
     6421 accacacctg ctaataaaag tgagcgaccc accgcagcaa taaattccct gtccgtcagc
     6481 agctgttgga accattgcaa ggtaaaatca ctaggtaaaa gcgattttga ccatttagtg
     6541 gagctggcat acaaaatcgt tccaataatc ggcagcaagt aatacaggac aaagcagaac
     6601 aaaatcggtt ttattaactt tttcacgtct gcaccacctt gcctttcttt tgaataatca
     6661 acattgccgt taccagcagg acacctaaag cgactgccaa cgcactgcct aaattaggct
     6721 tggcgaacac atctcccgac actaaagccg caattcgaat cgttaaaaga tttacattac
     6781 tgctggtcag ggcatacgct gtttcatagg tccccatgcc attagcaaat aagatgacaa
     6841 aggtgctggc aatcgatggt cgcaaaaaag gcaaagccac cttgaaccaa gcatacgctg
     6901 atgaagcgcc cagcaattgt gcggactcca gccattcctt tttcacttcc ttcatgctgg
     6961 gatacaaaaa caagacccct aacggaattt ggaaataggt atacgtgatg gtcaatcccc
     7021 accaagaata caaattgaag tccgctagaa atggcaaaat cattttcagc acacctacat
     7081 tccccaacaa aataataaaa gagaatgcca gcggaatccc agcaaaatta gctgccaaat
     7141 tggaaacaaa ggtgattttc tcctgcaatt tagtcgatag attcattaag cataaacata
     7201 aaataaacgt cccgattaac ccaatcacac tggagctgac agcaatcagt acgctgttaa
     7261 caaaggcctg tgtgtaatat ttgttgctga aaatatcact gtaattggcc aatgtccact
     7321 ggttagttaa atcactgtgc aagctgcaca gaaccataaa aagcagcggc agcagtaaaa
     7381 atgcacagaa gataaaggag gcaggaacca gaaccaagcg ttttttcatc tctttcatcc
     7441 cctcatcaat tcttgaatgt cctgcatttt ctctgacggt gcaattccta ataaccagca
     7501 aaacaatgga gccgccagca gctgtggtac agtttgttcc ttcagctttt ggcgtttatt
     7561 tgaaatcaaa taaaaaggaa cttcacgatg tgcagccaag ctgccgccat gcagtccgtg
     7621 cgcatccatc ccgtgatccg cagtcaccac gacttgataa cctttttgca gccattcagg
     7681 aaggtaaata gccaataatc tgtccgcctg attaaccgct gctgcatatt catgagaatc
     7741 ggcggtaaat ttgtgaccga catcatcaat attcatcgaa tgtacgacca aaaagtctgg
     7801 ctgatactga ttcactaaat aatgggcatc cgcaaataaa tgcgaatcag ggtaagtgtc
     7861 ctcactataa aagatgccat gctgaatcag tcgctcctcc tgatgctgga tgcgatcaac
     7921 caaacgatta tagggggctt gattgtacaa ctcgctgtac cagtggtaag cggcggctcc
     7981 tgtttttccg ccagcttcct taactaattc aaacagcgac cactctttgg atcgctgagc
     8041 atggtaatta ctatagatgc cattttcata ggctggaacg ccggtcatca gtacttcgta
     8101 taacggacgt gaattgctgg gcagctcact ttttaccttc aataagcttg cttgctggtg
     8161 ctcaactaaa tgattcatga acccaagctg ggcgacggct gtgtcgtagc gacagccgtc
     8221 tagtataact acaatcagtt tgtcaagtct actttgcata ggtcagtacc tccgcctgcc
     8281 aaagctctgg aagctctaca gaggtcttgt tccaagcttc attgtcttca actggcaccg
     8341 ccgcttcata agcatcctcc ggcagtaatt tttcagctac ttctttaggc agctctacct
     8401 tgcgaatagg tcgcgcatag ccctttgcca aattgatttg cccttcatca gataaaatat
     8461 attcccgtgc tagctttgcc gcattgggat gaggggcatt tttattgatt aaggttgtat
     8521 acccgctaat gatggagccg tctgttggca cagttacctc aaaacgctcg gagtcaattt
     8581 ggtcacgata atttagtcca ttgaagtccc aaacaactgc cacatcgatt tcaccttttt
     8641 caagatttgc tacgctggca tcaacggtgg acaaacggcc tttttctgcc atttttttgt
     8701 agtaattcag acctggctga atatcctttt cgtcacctcc gttggcaatc gccgcagcca
     8761 atacagcaaa ttgtgcttgg ttggcaatcg tcacatcccc cacgctaact tgatagtcac
     8821 cctcttgcag ctccttccag ctagtcggtg cttgcttcac attctcttta tcggtgataa
     8881 aggaaatggt tcccgtatag cttaacaacc aatccccatc atcatccttt gcccaatccg
     8941 gaatttcctc ccaatagttg gtcttataag gtaaggttaa ctctttgctt tcagccaacg
     9001 gaccgaagct gataccgaca tcaccaatat cggcggttcc attttttcct tctgattcaa
     9061 acttagccaa ttcttctgca ctggacatat ccgtatccac gtgacttaag ccgtattctt
     9121 tttcaaggtc gttccaagtg cctacccaat ttgcccaagt atccggcatt cccaagctgg
     9181 caatctctcc ttcttccttg gctttttcta taatcgtatc taaagagtcg tctgccgcag
     9241 ccgattgctt cgttgcttca cctgagccgc agcctgtcag caggatactt actgttgctg
     9301 cacaaaataa accgatagtc atgttacgaa ttttttcatt cattttcatt cattcttctc
     9361 ccctttcgat aattccattc taacaaagct ttgtaatgag agaatcagtt tcttgaaaag
     9421 gtttcatgaa caatgtaaag gttgtatatg gtttgtaaat ttcgacgaat tcccgttaac
     9481 cttctaaagc ttttgcggta tgatagtaga aaaaaggagg ctccctatgc agtattatga
     9541 tcagcatctc cacacgtatt tttcctttga ttcagaggaa aaatttgaaa actatttagc
     9601 ctatcagccg gagtttttcg tttctacgga tcactttgat ttaaacaatc catctaccgg
     9661 aaaagatgac attccggatt atccagccta tataaacaaa ctggactctt taaaagaaac
     9721 ctattccacc aaatttctgc gaggtatcga gattggcgtt gttccaggac aagaagagcg
     9781 gatcgtggat tacctcgctc aacatcctta tgatttaaag ctggtaagta ttcatcaaaa
     9841 tggccgcttt gattatatgg acgatgtggt tttaaccaaa gatcccattc aaactaccaa
     9901 ggaatatttt gatcagatgt tgcaggtctt gacggatttt ccagaaggag atattttgac
     9961 tcactttgat tatggattac gccgcttcac cttcacagcg gaagaattcg cggagcattt
    10021 tgaggctcag ctgctaacga ttttcaaaaa ggttgttgaa cgagaattgg cgatggagtt
    10081 aaatgccaag agcttcttga aatacggcaa cgaagacctt taccgttatg ccgttccttt
    10141 gtatcaaagt gtcggcggaa agctctttac cttaggctcg gatgctcatg ttgccgaaga
    10201 ttatcagctg ggctttaaaa aaatgagtca gctgttggca gactgtcagg tagaaaagtt
    10261 gctagtcatt caaggaaaag aacgccgcct gacgccgctg ccgattttgt gattcctata
    10321 taatagaaga aagtggtgaa aaaaatgagc aacctgtggg actacaatca agaggcaccc
    10381 atccattatt taattgcgcg acattgggat gcgttgaaaa ttgaggcggt ttgccgatcc
    10441 ttgttagcag ccgttccaaa acaacaactg gaaaattttt tggtggctga cagcttgcag
    10501 cgggaaaaag ttcaggctta ttttgcagcc tttaaagatc agcctttgga atatttgcat
    10561 gcgcagtttc atctttttta tcaagtagcc gccccagatg actacaacga tttacgagga
    10621 cagctgcaat tgacctttca agcggatgaa accgcctata ccgttttatt gggaatggct
    10681 cgccttggtg atcaggcgaa ggtcgagtgg cggattttcg atatttgatg gcaggatcaa
    10741 ggcgtttcag ttgcaaacct tcctctcttt caattagaaa aaccgcagca ggaagaagtt
    10801 tgtgtgctgg tcaccgatgc tggtgaaatt cgtctccgct tatttcctga ccaagctccc
    10861 ctggctgtag ccaactggcg ctccctagct gagcaaggcg tttatgacaa cacacctttc
    10921 ggctcgtgtc attaaggaat ttgttattca aggtggcgct ttggatggtt ctggtgatga
    10981 agctgtttcc agctatgagg gctttttcgc tgatgaagtg catcgcggac tttatcattt
    11041 taatggcgcc ttggccttag gggaccacgg cccccacacc aacggcaacc agttttttat
    11101 tgtgcaaaac acaaaggcgc aggctgacct gctaatgtaa agcaagcata cttggagctt
    11161 ggaggactac cggagctgga tggtcgttat actgtttttg gacaggtata ccaagggtta
    11221 gaaatcgtag cagcgatcgc agctcagccg acaaacagtg aagatgtacc gattgagcca
    11281 gtgttcattc gacaaattaa cttcgaaaaa acatcgtaaa aagacactca ctgacttcag
    11341 caaaagctct attcttgagc tggctgcagc tagtgagtgt ctttcattta tccataagat
    11401 aaagcttgga ttcaataaat aaataaatgt tttggttaaa gctgtgacca gcaaatgagg
    11461 aaccgtgcga gccagttgat ctgccaatgc aaacatttct ttaggtgaag gcgctggcgt
    11521 gtctgccaac ggatactcca gtcccaatgc ttggtaaccg tgcataccat agcagtggta
    11581 cggtagtaat tctaacggta cgaaccgatc aagatctgcc acaaaacaag caatcgtcag
    11641 cagctcggtc aggttcatat tgatacctgg aaccacagga actcgaattc gcacttcgca
    11701 gttaggatac tcaattaaaa agcgacgaat attcttcaaa atttgttggt tatctaagcc
    11761 ggtccattgt ttgtggagct tgctggtaaa catttttaca tccacaaaca atgtatccac
    11821 atagggagct accttttgga tttcattata agcaccacaa aagctggttt ccagtacggt
    11881 attgattcca atgtatttcg atttttgcaa aatttcttta gcaaaatccg cctgaaccag
    11941 tgcctcgcct ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa cagcatcctt
    12001 ctcaatctca tccattacct cttcagcggt catggtctgg ccaaatcctt tttcaatctg
    12061 cttgctttgt gattcgggaa tcgaacacca ggcacagcgt aatgggcagc cctttaaata
    12121 aacaacggtc cgtaaaccag caccatcatg tagagaaccc tgttctattc ttaatactgt
    12181 tccttccata tagatttttg aattacacac tagtttaaac taaaacacac aaggaaaaac
    12241 ttaccattta atcataaaat tagctcatat aaatatacta ggatgtaaaa tagactggtc
    12301 aaaatctggt caaaaaagag gactaataca ggtagtcctc ttttttgtta ttttaatatt
    12361 ctaaatcatt ttttattggt ttattccctt attcgatgta gactcaaatc actatatcac
    12421 tagatatcaa acggcgtcat acggaatcaa aaacatcaaa tttgacctgt taaatggtca
    12481 ttgaataaca ttcaaaatca ataaaacgga gtcagacacg gagtcaaaaa cgctgagagt
    12541 aaaaaatata tacagccttg taggtaaagt accacagggc tgtatatttt ttatttcaga
    12601 ttaccttaaa tgattccatt tactttttat ctcatttttt cctactagtt agtatcaaat
    12661 actctcctat tttctcaata aactattttt ggatacatgg tatctgaaac agctaaaacc
    12721 ttgtcattac agcaaaaaat cacgttatcc agactgtaga caaagtccat tttatggatg
    12781 atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg agtgatagct
    12841 atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac gattgatgat
    12901 ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt tgaatttatt
    12961 tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga tccagtgatt
    13021 ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg acaaaccatt
    13081 aaggaagtcg ataccaatgt cgcttaccgc tggtttttag gctact
//
LOCUS       DBKOIJ010000068        13121 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000068 DBKOIJ010000000
VERSION     DBKOIJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.070"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            78..686
                     /locus_tag="ACV5NJ_001567"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACV5NJ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            706..1734
                     /locus_tag="ACV5NJ_001568"
     CDS             706..1734
                     /locus_tag="ACV5NJ_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HHY6485660.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACV5NJ_001569"
     CDS             1731..2489
                     /locus_tag="ACV5NJ_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HHY6485661.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACV5NJ_001570"
     CDS             complement(2575..3315)
                     /locus_tag="ACV5NJ_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HHY6485662.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACV5NJ_001571"
     CDS             3376..4629
                     /locus_tag="ACV5NJ_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HHY6485663.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACV5NJ_001572"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACV5NJ_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACV5NJ_001573"
     CDS             5300..5626
                     /locus_tag="ACV5NJ_001573"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HHY6485664.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACV5NJ_001574"
     CDS             5694..6407
                     /locus_tag="ACV5NJ_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HHY6485665.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACV5NJ_001575"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACV5NJ_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACV5NJ_001576"
     CDS             7690..9555
                     /locus_tag="ACV5NJ_001576"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HHY6485666.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACV5NJ_001577"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACV5NJ_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACV5NJ_001578"
     CDS             11120..11821
                     /locus_tag="ACV5NJ_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6485667.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11983..12459
                     /locus_tag="ACV5NJ_001579"
                     /pseudo
     CDS             11983..12459
                     /locus_tag="ACV5NJ_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACV5NJ_001580"
     CDS             complement(12857..13066)
                     /locus_tag="ACV5NJ_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HHY6485668.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         4404 a         2202 c         2767 g         3748 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat a
//
LOCUS       DBKOIJ010000069        13098 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000069 DBKOIJ010000000
VERSION     DBKOIJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.071"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            62..1801
                     /gene="yfcC"
                     /locus_tag="ACV5NJ_001581"
     CDS             62..1801
                     /gene="yfcC"
                     /locus_tag="ACV5NJ_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HHY6485669.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1958..3142
                     /gene="iadA"
                     /locus_tag="ACV5NJ_001582"
     CDS             1958..3142
                     /gene="iadA"
                     /locus_tag="ACV5NJ_001582"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HHY6485670.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3253..3456
                     /locus_tag="ACV5NJ_001583"
     CDS             3253..3456
                     /locus_tag="ACV5NJ_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HHY6485671.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3557..4219)
                     /locus_tag="ACV5NJ_001584"
     CDS             complement(3557..4219)
                     /locus_tag="ACV5NJ_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485672.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4232..5734)
                     /locus_tag="ACV5NJ_001585"
     CDS             complement(4232..5734)
                     /locus_tag="ACV5NJ_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485673.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6282..7961
                     /locus_tag="ACV5NJ_001586"
     CDS             6282..7961
                     /locus_tag="ACV5NJ_001586"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HHY6485674.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8088..8567
                     /locus_tag="ACV5NJ_001587"
     CDS             8088..8567
                     /locus_tag="ACV5NJ_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HHY6485675.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8786..9466
                     /locus_tag="ACV5NJ_001588"
     CDS             8786..9466
                     /locus_tag="ACV5NJ_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HHY6485676.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9474..10805
                     /locus_tag="ACV5NJ_001589"
     CDS             9474..10805
                     /locus_tag="ACV5NJ_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HHY6485677.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10820..11782
                     /locus_tag="ACV5NJ_001590"
     CDS             10820..11782
                     /locus_tag="ACV5NJ_001590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HHY6485678.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11893..12231
                     /locus_tag="ACV5NJ_001591"
     CDS             11893..12231
                     /locus_tag="ACV5NJ_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HHY6485679.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12310..13017
                     /locus_tag="ACV5NJ_001592"
     CDS             12310..13017
                     /locus_tag="ACV5NJ_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HHY6485680.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4160 a         2317 c         2876 g         3745 t
ORIGIN      
        1 tcatttgatt atccattttt tattcatcaa aattactgaa aaaagaaagg aagaaataaa
       61 tttgaagaaa acaaattctt caggaaaacc gaaacgcgac ttcactaaat tatcgacacc
      121 ccatacgtat gtcattattt ttggtgtagt catctttgca tggatactga cttttgtcgt
      181 tccagcagga aagttcagca cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag
      241 tactcgtact gttttacgtc aagattcctt ccgttatgct tacgagctgg ataagtcgta
      301 tgtgtttgat caattagaag aactgcaaga tcatccagca gaacgtgaaa aactggatgt
      361 tccggaaaaa ggattggaaa aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa
      421 actagatgaa atctccttga cagatgatgt gctttacgat cagtacggcg aaaatatcta
      481 tgatacttct aaaaaattgc ataaaacagc caaaatatgg ggaacagatg attttggcgg
      541 ctttggtttc ttaaattttg tttttgaagg actagtatct ggagataagt atggatctgc
      601 tgtagggatc gctgcattga tccttgttgt tggaggtgct tttggtatta ttatgcgtac
      661 cggagcgatc gacgcaggta tctatgcgtt tatcagtaaa actaagggac tggagcgttt
      721 agctttaccg ctattattct ttgctttttc attcggtggc gctacattcg ggatggcaga
      781 agaggtaatt ccattctcaa tggtgatggt tccatttgtc attgcgttag gatatgactc
      841 gatcgtagca gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga cctcttggat
      901 gagcccgttt agcgtagcgg tggctcaagg gattgctgga atccccgtgt tgtctggtgc
      961 gacattccga ttgattatgt gggtagttgt tactgcatta gccgctggtt atatgatgat
     1021 atatgcagaa aaaatccgta aaaaaccaga aagttcgttg acttacaaat cagatgatta
     1081 tttccgttct catatcaaaa agacatctga tgaaaataaa aaattcatgc ttgggcataa
     1141 attgatctta ttagagatgt tagttgtcct tgtctggatc gtttggggcg tgactcaaaa
     1201 aggctattac attccagaaa tcgcttctca attctttgtg atgggattag ttgctggaat
     1261 cattgctgtt cttttcaaat tagatggaat gaaagtcaat gacatcgctt cttctttcca
     1321 atctggtgca gctgatttag ctggtacagc aatcgtagta ggtatggcca aagggattct
     1381 tcttgtcttg ggaggatctg atgcatctgt tccttcagct ttgaatacga ttttgcatgg
     1441 gatcgggacg gcgttgaccg gtgttccagc tgtcattggt gcttgggcga tgtacatctt
     1501 ccaaagtttg ttcaacttgg ttgttacatc aaattcagga caagcagctt tgactatgcc
     1561 aatcatggct ccactggcag atttagtagg tgtatccaga caagtagcag ttcttgctta
     1621 tcagcttggt tcaggtttta tggacgcttt tacacccgta tcagccagct tgattggtgt
     1681 gctaggtgtt gcacggatcg aatgggcaaa atgggcaaga ttccaaatca agatgcaagg
     1741 attcctgttt gttttaggaa ctattttcat catgattgcc attgcaatag gtctgcagta
     1801 gtactggaga cctgtaaata aaaaagtaat ccaaacagta gataaggact ccaagaaccg
     1861 tcattttacg gtaaaatcaa aggagtaaag atcctatcgt ttggattatt ttttaagatc
     1921 ctatcatcag ttatacttaa agaaagagga aaaatgtatg aaaatcattc gacaaattga
     1981 agtgtttgca ccagattact taggaaaaat ggatgtactg attgccggtg ggaagatttt
     2041 ggctgtagaa gaacagctag aaggcggcta cgaaggagtg gaagtagaag aactttcagg
     2101 tgagggaaaa gtactaaccc cgggtttcat tgactgtcat ttccatattt taggtggagg
     2161 aggagaaggc ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat tgactacagc
     2221 tggtgtaacg acagttgtcg gttgtttggg aacagacgga gaaggtagag atatgactgc
     2281 gttgatcagc aaagcgaaag gtctggaagc agaaggaatc tccacttatg tatacgaagg
     2341 ttcgtaccga ttgccggtaa aaacagtaac agattctatc atcaaagact ttttgacaat
     2401 tgataaaatt atcggaatcg gagaaatcgc agtatccgat caccgttctt ctcagccaag
     2461 ttttgaagag ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa
     2521 agctgggatc atcaatgtcc acttaggcgg aggaaaaaga aagctagagc tattgacacg
     2581 aatcgtagaa gaaacagaaa ttccgatcac acaatttttg cctactcatg caaatcgcac
     2641 accagaatta tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac
     2701 tgctagcgaa gatcctgatt tttgggagaa gacagatggt gaagttcgct tcagcaaagc
     2761 actgaaacga ttgatcgagg aagatgtttc tttagataat ttcacgatga cctcagatgg
     2821 acaaggaagc ttgccttatt ttgatgaaaa caatcatttc ctagggttag gagtaggaag
     2881 tgccaaggca ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta tccctttgga
     2941 aatcgcctta cgagctataa ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg
     3001 aaaaatcgaa attggtgctg atgcagatct gtgtatcttg gataaagaaa cgcttgatat
     3061 tgatacagtt atcgctaaag gagaaataat ggtccaagaa aaagaagtca aagtatgggg
     3121 gactttcgaa aaaagttttt aatcaacagc tctaactatc ttttttagaa aacgagcgtt
     3181 ctgtttgtct gtatctgttg ttgatgttac cctgaaggta gatcaataaa caaactaaga
     3241 ggaggaagca caatggcaga tttaaaagga cgttctaaag atatgaaaga taaagtagtc
     3301 ggtgaaggaa aagaagcata cggcaaagca acagacgaca agagcaaaga aacagaaggg
     3361 aaagcacaat cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc
     3421 aaagacaagt tttctcgaaa agacgaaaaa aaataaaaac cacttattac taattgaatc
     3481 aagcaaataa aaaatccgaa ccatatctgc gagacagata tggttcggat tttttatttg
     3541 atcattatcc tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga
     3601 cttcatcaca gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt
     3661 gttcatgggc gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat
     3721 ttccagtagg ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag
     3781 cgactcgctg ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat
     3841 caattcctac acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct
     3901 tagtgatttc cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt
     3961 aagcttggaa aataatcgag acatcatttt tacgataact tcttaacccg attttgttca
     4021 gtgaacgatc ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg
     4081 aaagaaaagt cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt
     4141 cgaaattcag ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca
     4201 attgttctgt ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta
     4261 atcttaatat ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc
     4321 cggctaatag tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag
     4381 aaacagcaaa tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt
     4441 gtctgttacc tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg
     4501 tctgttggtc aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag
     4561 caactcctaa tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt
     4621 tacttctgga ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg
     4681 taatcataat gatcagagta agtatgataa taccggctac agccactagt aaaacaacat
     4741 ttttagaaaa acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat
     4801 ttgattgtaa gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat
     4861 ccattttatc tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg
     4921 tcccatttag ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga
     4981 agttgaattt catacccata ctgccgcttg tttctctgga ttcataaatt cctttgatcg
     5041 tcatttcata agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg
     5101 ccaggtcatt tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc
     5161 tgtcatctat tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt
     5221 gtgcagttgc tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc
     5281 ccattcctcc agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat
     5341 cagaactagt gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg
     5401 aataactctt tacattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg
     5461 taagagaaaa actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt
     5521 gttcctgttt tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt
     5581 ctttttgtgc ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca
     5641 caaaaataag aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt
     5701 ttgtactttt ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca
     5761 tttgataata gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc
     5821 aacatgaggt tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat
     5881 gaaaaaagta tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc
     5941 tagatgctac agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga
     6001 tttagtttat ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc
     6061 tatagcgttt cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat
     6121 ccaactgtca gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa
     6181 atagtgattt tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc
     6241 gacatcttta aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta
     6301 tcccattagg cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg
     6361 atatttttat tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg
     6421 tcgtaatccc tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt
     6481 taacacatgg acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg
     6541 taccagtatt cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg
     6601 aagcagccaa aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg
     6661 gtgaaacagt agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca
     6721 atctgaaaac aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg
     6781 ctatcccaat gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct
     6841 tagccttact aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc
     6901 aaatagcaga tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt
     6961 gtgtagcaag taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac
     7021 gaaaagttgt cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag
     7081 aaaaacttca attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg
     7141 atccagaaga actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc
     7201 aaaaaatggc aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata
     7261 tcactactac gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt
     7321 atcgagctgg tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca
     7381 atcctaatga cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc
     7441 aaggagagta tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat
     7501 acaaaaatat ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga
     7561 gtgttgcagg aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata
     7621 tcggaaacat tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg
     7681 ttgtcactat cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg
     7741 gattcgttta cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa
     7801 caatcgttga gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca
     7861 tccgagatca attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc
     7921 cagtcattat ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa
     7981 cctctctata gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat ttttgaatac
     8041 agcatccctc gctttataat aaacgataga ataaagggag tgaatcaatg atgagtcaaa
     8101 caaaagtcga acgttattta gaagaacaaa agatcagcta tcaaccactt gattttagtc
     8161 atgtggtaga agaatctttc acagaagaac tacaaaaaag agggattgat ccagctttga
     8221 tatgtaaaac tctcgtagtg aaaggaaaca aaactggggt gatcatcgca ttgattcctt
     8281 tgaacgatca cttagattac aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt
     8341 ttcctggaat ggattttgta ttaatgcata caggatatcc ccacggagca aacaccccta
     8401 ttggaataaa actcgcacat cctgattatt gttttttagc agacagttct ttaaaagaaa
     8461 aagaagaagt aattgtgtca agcggtgaaa tcgggagaag ccttcggata aaagtagccg
     8521 atttagaaaa gatcgtccat cctatttacg ctgatttaat tagttaaggg acgagaagag
     8581 aagctaagca gagcagagac ctcaaacttg agattatatc tgcttggtac ttggtgcatc
     8641 ctgtattatc tgttacaatt gctttgtaca tactgtacta tcaatccaat gaaaatatat
     8701 ttagcagcta tattttttca ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat
     8761 acagagaaaa cgcgaggtgt aaaatatgaa aattacgtat catggacatt cctgtttaac
     8821 aattgaaatg gaaaatggcc aacgcgtgat gattgacccg tttattacag gaaatccatt
     8881 gacagactta actgcagaag aagtaaagac agattggttg ctgattactc atggtcatag
     8941 tgaccacata ggagatatga ttcctattgc aaaagaaaat caagccacaa ttatcagtat
     9001 tgttgagatt gctgattatg cgaacagcag aggagtagat tcattcggta tgaatatcgg
     9061 tggaaaacat gcgtttccat tcggcgtggt aaaattcgta catgcacagc atagttcaag
     9121 ctatgaagta gacggtatcg ttcagtatat gggcgagcct agcggaatta ttattcaagc
     9181 agaaggaaaa acgatttatc atgcagggga tactgcttat ttcagtgatc taggattgct
     9241 tgcagaagaa ttcgatattg atgtcgcttt cttgccgatt ggcgataact atacgatggg
     9301 gccagaagat gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca
     9361 ttacaatact ttccctgtca ttgaacaaga tcccgaatct tttgtagagt tactggaaga
     9421 tagtgaagga aaaataatga gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga
     9481 caaaacatga tttgatttta gaatatattg aaagtttacc tgttggcaat cgaatttctg
     9541 taagaagcat cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag
     9601 atgcagaaaa tgtcggacta gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac
     9661 gcaaattaaa aaaacatatt gaacgtttga catttggcga ggtcgtcagg atcattgaag
     9721 gggacgtttt aggcggggcg gcaggattag acaaggtctt gaataaattc gtcatcggcg
     9781 cgatgactaa acatgccatg gaacgctata ttacgccagg atcactaatg atcgtcggca
     9841 accgggcgga agtccaaaaa ttagcattgg aagatggcgc cgcagtattg atcacaggag
     9901 gattcaatac gactgctgag attgcaaagc tagctgatga tcttgaaatg ccgatcttac
     9961 gcacctctta tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat
    10021 tgatcaaaaa agatatcatg cttgtcagtg atatctatat gccagctgaa aagactcatt
    10081 atttgcagca gacagatacg atttctgatt atcagcgttt gtcagaagaa acgcagcatt
    10141 cgcgttttcc agtcgtcaac cgccatcatc gtttgatggg gattgttacg gcaaaagatg
    10201 ttttagggaa atcgccaaac cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg
    10261 taaagaaaac aatgagtatt gcctctgtta gccatcaaat gatctgggat gggctagaag
    10321 tcatgccggt cgtttccgat gacttgacat tggaaggctt gattacccgg caagatgtaa
    10381 tgaaagcgat gcagcttgtc cagcgacaac cgcagattgc agatacgatt tcggatcaga
    10441 tatctggaaa tatccatacg attgatactg accgtgaagg aaatcgtttg gaaaacccta
    10501 agttcaaatt cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg
    10561 tacttagcga aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa
    10621 atatcttgat tgaacaagtg aacctacatt atttgcggct gatccaattg gaaagcgaat
    10681 tagacattcg tccacgtgta ttggaaatcg gcagacgttc cgctaaactg gatatcgaag
    10741 tgtttattga aaacaccatc gtttccaaag caatcgttgt ttgtcaagta atggaacgtt
    10801 cttagaaagc aggagtgaaa tgacagtaca gcaagaaata gtagatacaa taaaaaaata
    10861 cgataaaatc attatccaca ggcatttgcg ccctgatcct gatgcattag gatcacaagt
    10921 aggattagcc gaactattac gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga
    10981 aacgattgaa gggcttcgct ttttagcaga aatgcaagaa gttgaagatg tcttctacca
    11041 aggagcgcta gtcatcgtga ctgatacagc taattcaccc agaatcagtg atcaaagata
    11101 tcagttggca gaaaaactga tcaaaatcga ccatcatccc aatgatgacc cttatggaga
    11161 tcttgtgtgg gtaaatacaa aagcaagtag ctgtagtgag atgattgcag aatttgcttt
    11221 gatgtttcct gaatctttaa agatgaacgt taaggctgca cggttacttt atgcaggaat
    11281 cgtcggagat acagggcgct ttttgtatcc ctccacgacg tcccgtacat tagagattgc
    11341 ttcgatattg agaaattatc catttgatgc aagtgctttg aacagagaga ttgaacaaat
    11401 gccgatgaaa gtcgctaaac tttccggtta tctttatcaa aatatccagg tggatgaaaa
    11461 tggtgcagga agaataatcc ttccgcaaga aattcttgat aaatatggaa tcgatgattc
    11521 agagacagct tcgatcgttt cacttcctgg agtgattgac gaagtgctgg cttgggggat
    11581 ttttgttcag cagccttcag ggtattaccg cgtacgttta cgttctaagg ggccgatcat
    11641 caatgaattg gctaaacgcc atcatggagg aggccatcca ttagcaagtg gtgcgaatgc
    11701 aaaagattta gcagaagtag aggaaatcta taaagagatc caagatatat gccaatcgta
    11761 taagagcaga aaaacagaat gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca
    11821 caaatgtatc ttttcaagat cttctttttc cgttacaata gtgctcacta aagattttaa
    11881 aggagaaaga agatgacatt accaaattgc ccagaatgtg gctcagagta cacatatgaa
    11941 gatcgaggct tgttgatttg cccagaatgc gggaacgaat ggaacaaaga agaacaagaa
    12001 actgtcatag aagacggatt agtggtatta gatgcaaatg gaaaccgtct gttagagggc
    12061 gatagcgtga cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa
    12121 ggaacaaaag tcaagaacat ccgtttagta gaaggcgatc ataatatcga ttgcaaggta
    12181 gaaggatttg gaccgatgaa actgaaatcg gaatttgtga aaaagaatta aagaatattc
    12241 aaggtctttt cacattcttt tcctactact aatgataaaa taaagtaaac gaaaaggagt
    12301 gaaacgaaaa tgtatggtta tggaatgtat ggttttttag atccgacatt tcttttggtc
    12361 attcttggat tagttatttc cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa
    12421 tacgatcaag tgagaagcaa aaacaatgtg actggtacac aagcagcaca atatatatta
    12481 caaagtcaac agataaataa tgtcggtgtt caacaaattg ccggagatct cacagataac
    12541 tacaattcag gtaataagat gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt
    12601 gctgcgatag gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat
    12661 gtcccattga agattcgtgc agcacttgtt ccagcagcta actttggttc gatgatttcg
    12721 ttccccttga ttatggtagg tgtattgctt agctggaact ctacgttaat caatatcggg
    12781 attttcgctt tttctttagc attacttttc caattggtga cattaccagt cgaatttaac
    12841 gcgtctcggc gagcaatcca aattttgagt gaaggcggac tactgacgga agaagaagta
    12901 ccgatggcaa gacacgtatt atttgcagca gcactgactt atgttgcagc agcgctttcg
    12961 acattcctgc agctgttgcg cttgatctta ttgttcggcg gcaaccgaag agattaaaat
    13021 aaagaagttg tgaaatcagc gctttgatct gagctaatac cgagagttag ctgattgaat
    13081 accatttatt tggaataa
//
LOCUS       DBKOIJ010000070        13083 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000070 DBKOIJ010000000
VERSION     DBKOIJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.072"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            65..232
                     /locus_tag="ACV5NJ_001593"
     CDS             65..232
                     /locus_tag="ACV5NJ_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HHY6485681.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(294..2105)
                     /locus_tag="ACV5NJ_001594"
     CDS             complement(294..2105)
                     /locus_tag="ACV5NJ_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HHY6485682.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2102..2467)
                     /locus_tag="ACV5NJ_001595"
     CDS             complement(2102..2467)
                     /locus_tag="ACV5NJ_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HHY6485683.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2630..3025)
                     /locus_tag="ACV5NJ_001596"
     CDS             complement(2630..3025)
                     /locus_tag="ACV5NJ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HHY6485684.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3043..4005)
                     /locus_tag="ACV5NJ_001597"
     CDS             complement(3043..4005)
                     /locus_tag="ACV5NJ_001597"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HHY6485685.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4005..4217)
                     /locus_tag="ACV5NJ_001598"
     CDS             complement(4005..4217)
                     /locus_tag="ACV5NJ_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HHY6485686.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4238..4936)
                     /locus_tag="ACV5NJ_001599"
     CDS             complement(4238..4936)
                     /locus_tag="ACV5NJ_001599"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HHY6485687.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4956..5498)
                     /locus_tag="ACV5NJ_001600"
     CDS             complement(4956..5498)
                     /locus_tag="ACV5NJ_001600"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HHY6485688.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5630..6634)
                     /locus_tag="ACV5NJ_001601"
     CDS             complement(5630..6634)
                     /locus_tag="ACV5NJ_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HHY6485689.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6631..7620)
                     /locus_tag="ACV5NJ_001602"
     CDS             complement(6631..7620)
                     /locus_tag="ACV5NJ_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HHY6485690.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7617..8423)
                     /locus_tag="ACV5NJ_001603"
     CDS             complement(7617..8423)
                     /locus_tag="ACV5NJ_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485691.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8589..9545
                     /locus_tag="ACV5NJ_001604"
     CDS             8589..9545
                     /locus_tag="ACV5NJ_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6485692.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9622..10140)
                     /locus_tag="ACV5NJ_001605"
     CDS             complement(9622..10140)
                     /locus_tag="ACV5NJ_001605"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HHY6485693.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10228..10392)
                     /gene="rpmG"
                     /locus_tag="ACV5NJ_001606"
     CDS             complement(10228..10392)
                     /gene="rpmG"
                     /locus_tag="ACV5NJ_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009554760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HHY6485694.1"
                     /translation="MGGKNMRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRE
                     RKVTLHRETK"
     gene            10605..11051
                     /locus_tag="ACV5NJ_001607"
     CDS             10605..11051
                     /locus_tag="ACV5NJ_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HHY6485695.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11246..13081)
                     /gene="asnB"
                     /locus_tag="ACV5NJ_001608"
     CDS             complement(11246..13081)
                     /gene="asnB"
                     /locus_tag="ACV5NJ_001608"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HHY6485696.1"
                     /translation="MMDRIVHRGPNSSGEYIDKHVALGFRRLSIIDLEGGTQPIYNED
                     RTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLLHGYEEWGTELLQKIRGMFAF
                     AIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIKSFLPHPDFNKELNKEALKPY
                     MTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQYWDADFETKETHSRQEWIEKI
                     DETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPDHSFSIGFDDKTYNEAIEARK
                     LTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPSCVPLYFLANLASQSVRVVQS
                     GEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTRYNLGRKIGKMKNFHGRIHLY
                     ESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDEIMTVHYEKTEGIKDEVNKMQ
                     YVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMKLAQQIPSKFLLNQNNTKDIF
                     RQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVKALFEADFAKEFFDQEKIMKL
                     LDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAEAIDYVTV"
BASE COUNT         4065 a         2783 c         2245 g         3990 t
ORIGIN      
        1 aactagcctt tttttctctt ttgttccata atagatataa caaataataa atgaggtgga
       61 ttcaatgaaa aaatttgtat ctggaatatt agtaggaagt ctggcaacag ccgctgccgt
      121 tgctggtttg gttgcttctg tgaaaaaaac agtgatcgat ccaattgacg aaaaagaagc
      181 aatgatcgaa gaaaaccgta aaaaagcaat gcgtaaacgc gtttctcgtt aataattatt
      241 taaaaatacg catgaagact ttctaaaaaa cagataagtc atcatgcgta tttttatttg
      301 tcagtcaata taagttgtgc tattttatct gcctgtctca gattccatgg atctgtataa
      361 ggtctagcta ctgctgcagt tagccaatcc acaccttcaa caggtattcc atagcaataa
      421 aagtcagact ggatttcccc tgaagcatca atcacgcgat tcgtacctcg ctcaacatct
      481 atagcttctg aatgataact tcctgtatta tctgaaaatg aaaaagggcg aacaagtttg
      541 ttttccttca aattttttat gacaggatta cttgaacgag aaaagcttgt ttgtggcaat
      601 cgagcttcaa tcaaatagcg actgctgatt tctttatctt gtagagaaat aacatagcgg
      661 ttgtgctttt gctgaatatc aaatggcggt tcgatcaaat gaaaaatacc cgcatctatc
      721 agtgctgcta attctttcgt ccgaatgatt ggcggaccga tcgttaaaaa gctatttaga
      781 tgagtaaacc atccccatag tatttctttg tattccttag cagaaaaaac gtgccattca
      841 atagcctgcc ggattggatc acgccagtct tttaatgcat caaatgttga agcaaatgct
      901 cctgtgcaat tgcctttttc cgcttctttt atctgccctt caataaatat tcggctagct
      961 tctgaaaagg gctcattctg ataggcaaga ggagtttcta aatacgacca atcccatcta
     1021 taagggatta gtgctggatg ctttccgaga cactcctctg aagagaggga gagaaaatca
     1081 tgctcaaaat ttttctttgg aatcggcaag tgatattctt cgatgatttt cttgtaataa
     1141 aacaactcaa cgtctttttt cagatagtca aaaaatgttt cgccagtcaa aaatcccgac
     1201 tgatgccatt gctccaggtt ttcatttgtt agtaaccgtg gccatgccat tgctccgcct
     1261 tgcttttgat ttcttcctcg agggtaataa gggagccccc ttcttgatcc actgtataca
     1321 atcggttcct tgcctgatgg atgatagacc agttttccct tttcgttctc gaagcgccct
     1381 cctcttgata ctgtaaacaa tccgacataa tcaaaaaaac ttaagcctag acctcttaaa
     1441 aagacatttt ctttaggcgg tatcttcgaa agatctacat ctgcaggatt agaaggaagc
     1501 tgatacagta aaacattttt ctttgcgtag tctacccaca ctctttcttc ttctgtctca
     1561 tcattttccc agtgaccgct tgctaaaaca agctgatcga tagaatagct ttgctgatct
     1621 gtctgtaaaa gataaccatc ttttcttttg cttacttttt ttactaaaga ataaacaaac
     1681 tgaatagaag ctggaaattc ttcttgcaac tttgagaaaa accagcgttg atagagtccg
     1741 tataaacatc gagtagattg ctgatctttt tccagacgat tgatttcttc tataattagt
     1801 tgcttatttt tggttaccgt ctgttcgata taatctgtag caggtccttt actccattga
     1861 aacaagttgg gtccctgtgt taccaatcct ccactggaca atgtttcatc tgtaaaaagc
     1921 gtcacttgtt gagaaacaga attcattaat aactccggtg gctgattcaa ccgccaaact
     1981 cgacctccag gaccattggg atcgaaaaga aaaagttcaa gagaatgtct gtcctgatta
     2041 ttatggagaa tcctttctgc tacacttaat cctctaggtc cggcaccaat gatccctatt
     2101 ttcatttgca tgcctccatt tcgataaatt ttttcaataa aacagcatgg ttctgatgtt
     2161 cattttttgc accataaaca aaagtaatga ctggatgttc tttcataagt tgactgagtt
     2221 taaaaatagc ttgcttcttt tcttcttcga aaatcaattc ttgctggtat ttctttgcaa
     2281 atgaagcaaa tcgttctggt acatggtcaa aagttttgcg tgcttcatta ctaggtgcaa
     2341 tttctttcaa ccacagatct aactgcgctt tttcttttga aattcctcgt ggccagaatc
     2401 tatccacgag tattctatag ccatcctctt tttcagcatc aaggtaaaca cgttttaatt
     2461 tgatcatagt tgtttccttc tttctacctt ttcgcttagc ttatatatgt agataatagc
     2521 acaaaataaa aaaaatgaac ggcacaacta tccattgcgc cgttcatgtg atcttggtca
     2581 aaatctaatg attgattcgg tttttaaagc tgtcatacaa tcatctgttt tatacttttt
     2641 tacttttgat ttttccagac catccgtcat atccaccctt aagtaaataa atctcttgat
     2701 aacctgcttt acgtaattta tttgctgtac ggatgctcaa acttttcttt tgatcgtaaa
     2761 tatagacagg ctggtctttt cgaatagatc ctaatgaatc tttcaaaaca gtgtaaggga
     2821 tgtttcttgc acctaaaata tgcccagcat caaacacatc tttttcgcga acatcgatta
     2881 cttgtgcttt tctcattcct tctcgaaatt cttcttctgt caaagtcgtt gctgaacgtt
     2941 tggccattac tcgtagatag acttcccaaa ggatgattgc aagcacaatc aatagcaaaa
     3001 tgatattaat tacccataaa accatgtaat gcctccgttt ttttaagcaa atttcaaggc
     3061 taatgaggca gcaccaatga caccagcttc attaccaagc tctgctaatt tgatttttgt
     3121 tgattctctt acttgcggga aagtaaattc tttaaaatat ttctctacac ggctacgtaa
     3181 aaattctcct gctgccgata ctcctccacc taacacgata cttgacggat ttaatgtatt
     3241 ccccaagttt ccgcaagcta aacctaagta gaagcatacg cgatcaacaa ccatcaatgc
     3301 aaacggatcg tcttcttgag ccatttcaaa gatatcttta cttgttactt cttctccgtt
     3361 gtctagcatt gctttcagtt ttgaatcccc tgcgtattct tctgccaaga aacgacctag
     3421 gcgtaccaca cctgtagcgc tagatacagt ttctaaacat ccgcgtttac cgcaagtaca
     3481 ttcgaaccct tcaggatcca cagtgatatg tccgatttct cctccagctc cgctcacgcc
     3541 gtgaatcaaa tttccgccag caacgatacc tccgccaaca cctgttccta gagtaacgaa
     3601 tacaacgtct ggatcgtttt ctccagcacc tttccatcgt tcacctaaag cagcaacatt
     3661 cgcgtcatta tcgatagcga atttgattcc tgttcctgcc tcgatcagtt ttttcacaaa
     3721 ttgaacttct gtccagttta agttatacgc accaataacc gttccagctt cgctgtccac
     3781 gcttcctggg gtacccatgc cgattccgat aaagtcttcg gctttcatat tatataaatt
     3841 caaacgatga ttgattgatt caatgatttc aggaacgata tgcttaccat catccaaaat
     3901 attggtatcg atgctccatt tttgctgaat ctctccttct ggtgttaaaa ttgcaaattt
     3961 tgctgttgtt ccacctaaat caatcccaat aatcttcttg tccattacaa atttcctttc
     4021 tttcggcttt cttcaattcg gtgctctctt tttaatatgc gccacgctgt catgtatgta
     4081 tcatgatcaa tcaaacggct atcatacaga ttacgcaatt cgatagtcat taattcaatg
     4141 tcatagatgc gtgcacccac atagataaag atgccaaatt ttttgaacag ctgctgtaca
     4201 tcatataagc tctccatctt taagcacttc cttcatatta tacaagtaaa ccatattttt
     4261 ttaaacccag taaaatacat attacaaaaa gaaatacaaa aactagtgta gaaagaatcc
     4321 tttggtgtat cgaatagctt gtctgttgtt tcggtagacc caaaacattc cctaacacta
     4381 atccgccaat tagcccaccg acatgtcccc aaacatctac agaggtatcc agaagaccaa
     4441 aaacaaatgt caacacaata aataaaagaa aatgtctgac tagctgctgg atcgctgggt
     4501 tatgtttaaa atgaaaccct aagataaaca acgcaccgaa caaaccgaat atcgaagtac
     4561 ttgcaccacc agacagaaca ttcgcttcgt taaaagcaaa gcttgctgta tttcccatta
     4621 tcccgctgaa catataaatc aagaaaaaac gccaatggcc atagattgcc tcgatttgct
     4681 gccccataaa atacagtatc agtgagttca ttacgaaatg catcagtcca aaatggatga
     4741 agattggtgt cactaaacgc caccattcat tgaagtgaac aagataagga ccatacattc
     4801 cggcataata agggatctca agtcgcggaa ataattccat catgaggaag accgctgtct
     4861 gtatccctaa aaaagcatag gtccataacg gtcgtcttaa ccatacacgc aattttattt
     4921 gctgttgata attcatgaaa acctcccatt tcattttagt caataaacaa ttgttggatc
     4981 ttctgatcaa agctctccgg ttgccattgt tcattcaatt gttcacgaaa aacaaggcta
     5041 catgtttttt tatgaaaatt atttaaatac cggtcgtaat aaccaccgcc aaaaccgatt
     5101 cgataaccgg ctctattgta gataatacct ggtacaatga ggagatcgat catctccggc
     5161 aaatagagct tctctgattg tggctcaaga acaccaaaac tcgaacgtac aagaacggac
     5221 tgttcatcca tttcataaaa cgccatctga cgtttcggca aagttttagg tacaactatt
     5281 ttcttttgtt ctttttctgc tcgttggata accggctgtg tatccagttc aaattcattc
     5341 gataaagtcg tccctatcac ttttgctgac ttccagtact cgctggcgaa tagctgttta
     5401 tagattcgct gttctttttc ctttttcttg ccacttttgg ccagattttt cagcttttcg
     5461 attcctttcc ggcgtaatct tactttcaat tggtccaaaa actcaccctt ccttctctca
     5521 taaaatggta taggaaaaaa agaaaaatag caatcgaact ttcaaataaa tgcataaata
     5581 gaataaaaag aagacctaga aatgattggc cttccttttt taatgaattt caactgttca
     5641 gcgcgtgttt ttgtctgata aaaagtgcat acagaaaata tggcgcacca aaaagggcaa
     5701 tcaaaattcc tgtaggtact tccccactta gtagaatcac tctagcaacc atatctgcac
     5761 taagcagaaa aatggaaccg aacaaaccgc tcagaacaaa ggtccagtta ttttctttac
     5821 gtacgactag cttcgcagca tgaggagcta gtagtcctac aaaagataaa ctacctgtca
     5881 ccgctacact accgcttgcc aaagcaacag ctaacaacaa aaggattttt tgacttttag
     5941 ccacgttaag gcctaatcct tgcgcgcgtt cttgaccaaa tgatagcaat acaatcctct
     6001 gtgcataaag tcctcctagc ggaattaaga taaccgccca tggaagcaat gcccaaacat
     6061 aaggccagct cgcaccccag attgatcccg ctaaccatgc attcacaaat ccataatttt
     6121 ccttagataa ttggatcgtg catagcaatg ttaatgcaga aaggccggca ttaacggcaa
     6181 taccggacaa taagattcga ttcataccca aaaagccatt tcgatcatag gccgcaagat
     6241 agacacagca agcagctaaa acaccaccta gaaaggctac aactagcaat agaaacggca
     6301 gcgaatagtg cgaaaagaaa cctaaataaa gcatcactaa aaatcctgct ccggcattaa
     6361 tgcccaatat actagaatct gccaattcat ttcttgttac tccttgcaag atataaccag
     6421 aaacgccaag acaagctcct gctaataaac tgatcagtaa tcgaggaact cgaaattctc
     6481 taacaatcag cgccaaagaa gatgattctt tccctatgaa cgtggcaaaa agctgatcca
     6541 ggtaaataaa aggcgtacca atcattaaac tacctataga caggatgaca agtacaaaaa
     6601 gcaagaaata gaacgtccaa tatatttttt tcaccgttgt tccctcctta cttgtatcag
     6661 taaaaacgga acgccaatca atgcaatgac taatccaaaa ggtgtttcaa aagggggatt
     6721 gatcatcctc gctgtaaaat ccgccaacat aaccaatcca ccgccaacta aaaaagagac
     6781 aggtagaaca taacgatagt ccgttccaac cattccgcga acaactggcg gaaccaacag
     6841 cccaataaat gaaacaggtc cgactaacga aacggcaatg gctgctaaca ctgcaactag
     6901 acagaaaaca atcaaccgcg ttcgtttagg atgacttcca agagctaatg catgtgtttc
     6961 actcaaatga atcaaagata ttgcttttcc taatgaaaaa gaaaccaaaa cagcaagtat
     7021 atataaagga aacaatactg ctaattgttt ccatgtgata ttggcagctc cgcctacaaa
     7081 ccaaaaagca agatcctgct gcagattaaa gaattgggta agtgcttggc tgatagcagt
     7141 aaaaaaagaa ctaatagcta cgccagctaa aatcagctgt aaaggattca tgcccatttt
     7201 cgaaaactgc aaaaaaccaa atacgagcaa agttacagca aaagaacctg ccaatgacat
     7261 tagaaacaca gaaaaatcag aagctttagg ccaaaaagca aaactaaggg ccattgccaa
     7321 cgaagcaccc gcgttgattc ctagtaagcc tgaatctgct aaaggatttc ttgtcactcc
     7381 ttgcatcaat gcaccgctca atgcaaaaca actccctaca agaaatgctc ccgccacacg
     7441 aggcaaacga atatttcgta tgatttggtg actttgtttt gatctatcaa aatcaaacaa
     7501 cgcctgccaa aaatctgctt ttcccacagt cgctgcgcca tttgaaagag caacaaaaaa
     7561 aataatcaaa agaacaaaac agaccaaaag caggagtcta tttttcttca tagtcctcat
     7621 aattgatcct cctgctcttt tggcaacaaa tcataggtta gaaaaactgg tttatttgtt
     7681 cttggaagcg tgacaatcgt tgcatcaatc tcaaatattt ttttcataca ctcttttgtg
     7741 atcacttctt caggtgtacc ttctgtgatc aacttaccgt ttttcattgc taataaataa
     7801 tctgaaaaac gtgaagcatg attaatgtca tgaatcgaca tcaagatcgt tgtttgtttc
     7861 atttgattga tttcagtcaa taactgtagg atctctaatt ggtgtgccgg atctaaaaat
     7921 gttgtcggtt catccaagat caagatatct gtttcctgag caagcgccat agcgatccag
     7981 acacgctggg tttgtccacc agacagcgtt gcgatatccc gcatacgaaa tccagctaga
     8041 cctgtagcat ccaacgccca ctcgattgct tcatgatctt gcgtgtccat gccactgaac
     8101 agtttttgat aagggtatcg tccgtaagat actacttctt ctactgtcat acctgtaggg
     8161 tgttcactag tttgtgctaa taatgcaatt ttttttgcaa gttcttttga actgtatgta
     8221 gagatatcag agttattgac aatgatcttt cctgatttca caggtagtat acgggtaaaa
     8281 cttttcaata aagtagattt tccacttcca tttggcccaa tcaaactagt gattttccct
     8341 tcaggaatcg taacagacat gttttcaatg atttttttgt tttcgtagcc ggctgcaatg
     8401 tcagctgctc ttaatctcgt catttcaccc atcctttcgc tgaaaatcat tctcaattat
     8461 gacgctgttt caacatttcg tcaagcgcta ctttcatatc tatagccaaa tgaagcggtt
     8521 gacagcgttc gaaatatcag gaataataac caatgagaaa gattctcaat tatgttagga
     8581 ggaaagaaat gaaattaaaa acaaaagtat tcacattatc tagcatattg ctgctggctg
     8641 gagtactgag cgcttgctcc ccctcttcag ataaagaaac agctacaact gccaattcga
     8701 cgacttcatc caatagtact catacagtca cagatacact tggacataaa gtagagattc
     8761 caaacgcgcc caaacggatc atcggcagtt acttagaaga ctatctgatt gcactagggg
     8821 aaaaacctat cgcccagtgg actgttggca gtggttccat tcaagattat ctgcaagatg
     8881 atttaaagga tgtcccaacg atttcttatg accttcctta tgagaaagtg ttaagctttg
     8941 agcctgattt gctgttgatt tcttcttccg ctactgtcga aggcgggaaa tacgaacaat
     9001 atagcaaaat cgcgccaact tatgttgtaa aaaacggaac agatgtcaca tgggaagaac
     9061 aactaaaaga tgtcggcaaa gctttggcaa aagaaaaaga ggcagaaaag atcatttccg
     9121 attatcaaaa atccatcaag aaaactcgag aagaactagc agataaaatc gagaacaaga
     9181 cagctgcagt gttgtgggtg accaacaact cagcctttat ggtcagcaaa aatcggtcaa
     9241 gtggaagaat cgtatacgac gatctggaat ttggtgttcc tgatctagtg gaagaggtct
     9301 ccaaaaaagc cacttctgac tggtctgctg tttcctctga aaagctagct gaattagatg
     9361 ctgactacat catccttgtg aacagtgaca aagatgctgc catgttcaat gaaccaagct
     9421 ggaagaatct taaagcagtg acagacaatc atgtactgca attcggacct gaatctagtt
     9481 ggctatataa cggacctatt gccagtcagg aaatggtcaa tgatatcaaa gaaaaactaa
     9541 attgatttta actaaaaaaa gcatcttcac gattagtcgt gatatgcttt ttttcttatt
     9601 aacaaactag acgcatagat tttattcttc ttttgaagca accctatttt ttactctttc
     9661 ccatttttga aaacgatggg catatagatt ttcttgatcc gttatcccct catactcctc
     9721 tataagggcg aagtctgaaa aatcaatatt cccaatatac gtatcgcctt caaacgtgct
     9781 atcgatcaat gttcgccaga tccgaccagt gtaaggaaga agttcttgaa aaatccgact
     9841 accacccgaa acatagatat cttctgagat cgtttgacta tacgacaaaa gttcatcgat
     9901 cgaatgcatt acttccgcat tttctttcgt gcgaaaattt ggctgcgttg tcaacacgat
     9961 gatatggcga taaggaagtg aaagatcccc cattccttca taggtctttc ttcccatcac
    10021 taaaattcga tccattgtat gttcgcgaaa gaaacgcata tcatttggca atcgccatgg
    10081 taacaaacca tttttgccga tcagaccatt tttatcttgt gcccacatgg aaataaacat
    10141 attatccgct ccttatgatt ttagatgttt tgagataaaa aaagtctcaa ttctttgacc
    10201 agcaaggaat tgagaacttt aaaaacatta ttttgtttca cggtgcaaag taactttgcg
    10261 ttcgcgtggg caatattttt gtttttccaa acgatcagga ttgttacgtt tgtttttgct
    10321 tgttagatag ttacgttctt tacaagaagt acactctaaa gtgatgttta cgcgcatgtt
    10381 tttccctccc atttttacaa gaatcctcta agatgactct cagacttgat tatcttacca
    10441 tgattttttc tagattgcta gtgtttttca cgattttgtt aagtaaaact acttgacgat
    10501 aaatacaacg gttttacttc tcaaacaaaa tctttttctt tttgtataaa tacccttaca
    10561 cttgtttata tagattatgt tttttaataa agggggatac taatatgcca tggaacatga
    10621 aggactttcc tgcatcaatg aaaaacttag ataaattgac aagaaaaaaa gcaatcgaca
    10681 tcgcaaatgc attattagat gaagggtacc ctgataaccg tgcgatccca attgccattg
    10741 atcaagcaaa aaaatggtat gaagatgcta gtgaatcaga aaaacgaact ttcgaaaaag
    10801 aaaaaaatcc aagcaagacg gatgaacacg atacgaatcc tcgtgctgga aaactgcttg
    10861 attcagatgt gatcgtagaa tatgaggaag aacaatggat cgtgaaatct aaaggggcaa
    10921 aaaaggcaag caaccatttt gatacaaaaa aagaagcaat cgaaaaagga aagcaagtag
    10981 cacaaaacaa agaatcaact ttgttcattt ataaaaaaga tgggacaaaa gaaaaagaaa
    11041 tcgattattg aacatatgta aaggggttgt gacgaaaatc gtgtcacaac ccccttatgt
    11101 tcgaaacaat ggtgttcctc tgccaactcc gaaccataag tcataatatc tataaaacgg
    11161 gaagagctgt tttttgaaat tgtttcttat cactcagtgt taaacggtag gagaaaacct
    11221 cttttttatt gatatttgat tttcatcaaa cagtcacata gtcgattgct tctgcttgcg
    11281 gaatcgtttc atctacaaag tagactttat accatgtcaa gaaactgaag attgtccaga
    11341 tttttctttg ctcttcttta cgtccttcgt gatggtcatc tagcaacttc atgatttttt
    11401 cttgatcaaa aaattctttt gcaaaatctg cttcgaacaa tgctttgact tgttcgtagc
    11461 cgtgttcttc tttcaaccaa gctttgattg gcacagggaa tcctaatttc acacgattcg
    11521 accatttttc cggaagatgt ttgttcgcag cttgtctgaa aatatctttt gtgttgtttt
    11581 gattcaataa aaacttactt ggtatttgtt gagccaattt catgacttcg atatccaata
    11641 aaggtactcg gacttccaaa gaacttgcca tagaaagttt atctgccttt aacaagatat
    11701 cttttggcat ccattgatga agatcgacat attgcatctt attgacttca tctttgattc
    11761 cttctgtttt ctcgtaatga acagtcatga tttcatccac agatggtgct gtttgatatt
    11821 ttggtgttaa cacttctgat gcttctgatt cttcgaacac tcgagcatga ccgataaaat
    11881 attcttttgc aggagcaagt gattcatata aatggatccg tccatggaaa tttttcattt
    11941 ttccgatttt acgtcctaaa ttatagcgtg ttccttttgg taattttttc aatccttgag
    12001 caatcacacg gatgaatttt gaattcgtat ggaatccata cgtttggtat cccgcaaaca
    12061 gttcatccgc cccttctcca gattggacta cccgcacact ttgtgaagca agatttgcta
    12121 aaaagtacaa aggtacgcaa gaaggatttg aatcaggttc atctaagtga tactggatca
    12181 aagggaatgc tttgaacgac atgtctccgt cgatcactgc tgctgtatta tctaagtcca
    12241 atagttcggt tagttttctg gcttcaatcg cttcattata ggttttatca tcaaacccga
    12301 ttgagaaaga atgatctggt ttcaatacag aggttacata actggaatca accccgctgg
    12361 ataagaaaga accaacttct acatcactga ccgtatgtgc ttcaatagaa gcttgaacgg
    12421 tttcgtcgat tttttcgatc cattcttgtc gggaatgcgt ttcttttgtt tcaaaatctg
    12481 catcccaata ttgctgaatg tctatttttc catctttata cgtataataa tggccttcag
    12541 gcaaacgata gacatctttg aagaatgttt cgccgtttaa tggagaatat tggaaagtca
    12601 tataaggttt caacgcttct ttgttcaatt ctttgttaaa atcaggatga ggcagaaaac
    12661 ttttgatttc tgaaccaaac ataaatgttc cgttcatttc tgcataataa tatggtttga
    12721 tcccaaagtg gtctcttgca ccgaataact cattcttttc attatcccaa atagcaaaag
    12781 caaacattcc gcggatcttt tgcaataatt ctgttcccca ttcttcgtaa ccatgcaaaa
    12841 ggacttctgt atctgcatgt gtttgaaaaa cgtggccagc tgcaatcaat tcttctctca
    12901 aaggctggta attatagatt tcgccattaa aaataatgat ctttgtccga tcttcattgt
    12961 aaataggctg agtaccgccc tctaagtcaa taatacttaa tcttctgaat cctaaagcaa
    13021 catgtttatc gatatattct cctgaactat taggtccacg gtgaacgatt cgatccatca
    13081 ttg
//
LOCUS       DBKOIJ010000071        12920 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000071 DBKOIJ010000000
VERSION     DBKOIJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.073"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            132..3659
                     /locus_tag="ACV5NJ_001609"
     CDS             132..3659
                     /locus_tag="ACV5NJ_001609"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HHY6485697.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="ACV5NJ_001610"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="ACV5NJ_001610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HHY6485698.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="ACV5NJ_001611"
     CDS             7447..7788
                     /locus_tag="ACV5NJ_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HHY6485699.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     misc_binding    7832..8043
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="ACV5NJ_001612"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="ACV5NJ_001612"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HHY6485700.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /gene="pheT"
                     /locus_tag="ACV5NJ_001613"
     CDS             9145..11565
                     /gene="pheT"
                     /locus_tag="ACV5NJ_001613"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HHY6485701.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ACV5NJ_001614"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ACV5NJ_001614"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HHY6485702.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4390 a         2244 c         2739 g         3547 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta
//
LOCUS       DBKOIJ010000072        12783 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000072 DBKOIJ010000000
VERSION     DBKOIJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.074"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..177)
                     /locus_tag="ACV5NJ_001615"
     CDS             complement(<1..177)
                     /locus_tag="ACV5NJ_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6485703.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
     gene            319..933
                     /locus_tag="ACV5NJ_001616"
     CDS             319..933
                     /locus_tag="ACV5NJ_001616"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6485704.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1122..3002
                     /locus_tag="ACV5NJ_001617"
     CDS             1122..3002
                     /locus_tag="ACV5NJ_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HHY6485705.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3081..3308
                     /locus_tag="ACV5NJ_001618"
     CDS             3081..3308
                     /locus_tag="ACV5NJ_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HHY6485706.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            3313..3855
                     /locus_tag="ACV5NJ_001619"
     CDS             3313..3855
                     /locus_tag="ACV5NJ_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HHY6485707.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4082..4798
                     /locus_tag="ACV5NJ_001620"
     CDS             4082..4798
                     /locus_tag="ACV5NJ_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HHY6485708.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(4858..6693)
                     /locus_tag="ACV5NJ_001621"
     CDS             complement(4858..6693)
                     /locus_tag="ACV5NJ_001621"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HHY6485709.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6889..7887)
                     /locus_tag="ACV5NJ_001622"
     CDS             complement(6889..7887)
                     /locus_tag="ACV5NJ_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485710.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8106..9326
                     /gene="sstT"
                     /locus_tag="ACV5NJ_001623"
     CDS             8106..9326
                     /gene="sstT"
                     /locus_tag="ACV5NJ_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HHY6485711.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            9473..10108
                     /locus_tag="ACV5NJ_001624"
     CDS             9473..10108
                     /locus_tag="ACV5NJ_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HHY6485712.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            10299..11039
                     /gene="truA"
                     /locus_tag="ACV5NJ_001625"
     CDS             10299..11039
                     /gene="truA"
                     /locus_tag="ACV5NJ_001625"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HHY6485713.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11184..12782)
                     /locus_tag="ACV5NJ_001626"
     CDS             complement(11184..12782)
                     /locus_tag="ACV5NJ_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485714.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4276 a         2033 c         2505 g         3969 t
ORIGIN      
        1 atcatataga tcatagatat ccttataatg tgtataaaaa gtcgaacgat gtacatctgc
       61 cgtttctgtt agttcgcgta tagtgattgc ttgaatcttt ttatttacta ataatgtgga
      121 tagtgtttca gccaaagctt tttctgtttt tttgacgcgt ctatctgtct ttttcattta
      181 cctatctcct tatccgacat atcaaagcaa agctgtcgct taatcgacaa acttatagat
      241 gattgattat agatagtaga cactgtgtct attataatgt aagtataaca caatcattga
      301 agttgaggag gcgaaatgat gaaactaact tttaaaccct tagaaaagaa agattacaaa
      361 aaagctatct attttgctgt tgaaggaatg aactttgaga gatatatgga aaatgaactt
      421 ctgctgaatc tttacggaag atattttttg tatatggagt tgaatcgagc aactcaaatt
      481 cttgctttgt atgcagagga cgagttggca ggtgttttgc tggcggagat aaaaggagaa
      541 aaaaagaaac atcgttcttt tggacagcaa atatatgtta agatattcga ttttttacaa
      601 aatatatttg ctaaagaaag taaaggagct tacgagacag caaatgaaca aatgcttgca
      661 gcatatttaa agaagcattc gccagatgga gagatcattt ttttagctgc aaatcctgat
      721 ttgaaaacaa aaggcatcgg taccaagttg ctaacagaat ttgaaaaaag agagaaagga
      781 aaagaaatat tcctttttac agatagtggc tgtacgtatc aattctatga acgacgtgga
      841 tttgaaagac taaaggaaaa aattagcaag ataaaaatct tagataaaga aatagatctc
      901 cgatgtatgc tgtacaggaa aatcattcaa tgaaaatttc gatagaaacg acaatagttg
      961 gcaagatcac ttttagtgag tcttcttgct aactattttt tgtagtgtat tctaccaaaa
     1021 gaaaaccgcc tttttgacta aacttgatag tggtcaattt tttatgatgg ataagtatct
     1081 atacttagtc tcgtaagaat gaaaggaaaa gagggagaaa gatgaaatta caaaagtgga
     1141 tccaagagca tcgaaatcat atcaccgcct tatcagctgt gttgattatt tttggtttta
     1201 tcggaaaatt tactggtttt gtcttgttct ataacattgc tctagccata gcttctatta
     1261 ttgcggtcat acctattatg atccatgcgt atcaggcagt gaaagtcaaa gttatctcaa
     1321 ttgatttact cgttactatt gctgtgcttg gtgcctttat gatcggagaa tacaaggagt
     1381 cagctatcgt tacattcttg tttttgtttg gtagctatct ggaacaaaaa acattgacca
     1441 aaacgcggga gtcaatcaag gctttgaccg agatggcccc atctactgct agtgttgtcc
     1501 aagaagatgg aacgactgaa gaagtggaaa tcgatgacgt ggaagaaggc gatatcgtct
     1561 tagtaaaaac tggtggagct attcctgtgg acggacgagt cattgacggg aaagggtatg
     1621 cggatgaagc cgcagtgact ggtgaatcac gacaagtcgt caaaatcagt ggagatgaag
     1681 tcttctctgg aacgatttta gcagatggct acgtgacgat tgaagcaact aaggtcggag
     1741 atgatacgac ctttgccaaa attatcgaat tagtagaaga agcacaagat accaaatccc
     1801 atgcggaaaa attcattgat cgattttctc agtattatac tccaaccgtt ttacttattg
     1861 cgttggttgt tggtctgatt agtcgtgatt ttcgtttagc aatcactgtt ttggttcttg
     1921 gttgtcctgg cgctttagtc attggtgcgc ctgtttcgaa tgtagcagga atcggtaatg
     1981 gagcgaaacg gggcatctta gtcaaaggcg gtgaagcaat ggatactttt tccaaaatcg
     2041 atacttttgt ctttgataaa actggtacat tgaccaaagg aactactgct gtatccacta
     2101 tcaagaacta tacagaggat gaaggggaca gcttagcttt agtggctcga ttagaaacgt
     2161 tatccgatca tcctctgggg cgcgccgtga tccattatgc tcaggagaaa gacattgatt
     2221 ttcaacaatt agcagtcaaa gataatcaga caatcaaggg acaaggaatc acagctgaga
     2281 ttgatggtca tacagtttgt gcgggcaaca ccaaattgat tgccgctcac aaactttctc
     2341 tcacaccaaa gcaagtacaa gatttacatc aattacaaca aacaggcagc tcggtcatta
     2401 ttgtagcaat cgacaaaaaa atcactcaaa tcataggtgt gtcagatgtg attcgaccag
     2461 aagtcgctga acaattagct aagttgaaac aagcaggagc aaaacagtta gtgatgctga
     2521 caggtgataa tcagatgact gctgattatg tagctgatat gttaggcatt gatgaagtcc
     2581 atgctgaatt attaccagac gaaaaggttc aatttgtgaa aaaataccaa gaacaaggat
     2641 tgcgcgttgc ttttattgga gatggaatca atgattctcc ctctttggct gcggctgata
     2701 tcgggattgc tatgggatca ggaacggatg ttgctatcga aacgagtgac gttgtcttga
     2761 tgcaatctaa ttttgaatcg ttggttcatg cctaccgctt ggctaagaaa actgttttaa
     2821 atacccgtga aaatgtagtg attgccattg gtgtcgtctt atttttactc aacggcttat
     2881 ttgccgggtt tatttacatg gctagtggga tgttcgttca cgaagctagt atcttgatcg
     2941 ttatctttaa cgcgatgcgt ttattgtatt ttggacacga aagcaaagaa gaaaaatcgt
     3001 aaaacttgat aagaatcaat ttttttatgg aagctgtaat ctagaataag actatcaaga
     3061 aaaaggaagg aagaactacg atgaaaaaaa ttattatgca attagataca ctagcttgtc
     3121 caagctgtat gcaaaaaatc gaacatgcgg tttctcaaca gcctggggta agcaatacga
     3181 aagttttgtt taatgcgagc aaaatcaaag caaactttga tccagatgaa gtccaccctg
     3241 aacaattagc tgcagttgtg gaaaaattag gttatgaagt ccaatcagca aaagtaaaag
     3301 aggcgtaaat caatgaatat agtggaagaa aaatttgttc aagaaacaaa acaagcagat
     3361 atcgaccatc ataaaccaac tgctggcgcc atggtggggc atgtattagc aaattttaaa
     3421 ttagaagaaa tgaaattgat ccaagcacaa ttgtatgttt tgggtacatc ttcgcaagaa
     3481 ttaagtaccc ttttttcaga atatgctgat caagaagcga gatggttcaa taaaatctct
     3541 catgagttag tgattgaaaa tgaaatcgtt ccgtctactt tagaagaagt catgcgttat
     3601 ggaaaaattt acgaacatgg tcaaaacaaa tatttaccag cctcaacaat gttggaaaac
     3661 tttgcaaaag attttgattt ccaaaatgtc tttatcacac gggcaatcaa attagctgaa
     3721 aaagaagaaa aatatggctt gcaacaattg cttgttgaac ttttaagttt caataaagaa
     3781 atcatctacc aagtacaaag actccaagga aaaactgtcc gacaagattt ggatgaggaa
     3841 gacgatgatg actaaaagtc tatgtttctt caaatacaaa tataaagcgt gaggtaaatt
     3901 ggaaaatcac gaagattaaa ataaaagaag cacccgctga tgtattacag acaacatcag
     3961 cgggtgcttc tcagttttta gaagatttga aagattgatg agaaaatacg aaaaatcaat
     4021 aaaatcacat tgcttatttg atagaataaa gaagtgggag taatcgaaag gagtttttta
     4081 attgaaaaaa aggattttag taacaattgg tctagctttt ttgttagctg gttgtaccaa
     4141 cgggaatgaa tcgaaaaaag aatcaacaaa agaaacagcg aaagcaacag ttgcaactac
     4201 tacaacaact agcacagaga gcaagtcaac tgcagcaagt acagaaacag cagatacctc
     4261 tacttcgtca gagtcttcca aaacgactga aacaactcaa acatctactt caagttcttc
     4321 tgtagaggct tcatcttcct cttcagaaag cagcgaacaa gttgaacaaa cactgtggaa
     4381 tgcagacaaa tccgcacaat tagaggcatt tatgaccaaa tggggccaaa caatgggaca
     4441 acaatacaaa agttatacga atcaaatgag tgtggactta tatggtctta aagtccctca
     4501 agctattttg aacggggagt ggaagatggc tattggtgga gtgcctgttt ctgcagaatg
     4561 gtcagaaagt gggacaggtc aagcagacta tcaaatcaca gccgtctatt ccgatgcaga
     4621 gacagaacca tatctgaaaa agcacgtcta tctattcggg tttcagcaaa atcagccaaa
     4681 agtattggtg acacaacaaa atcaggggaa tcccgataat tatctttact tcaacgagac
     4741 agcgaataat gaattgaaaa atggctttaa tcaaatcgtt tatggacaag caaattaatg
     4801 agataaacaa ggggttgtga catgtctttt atcacaaccc cttgctcagt ttatttgtta
     4861 gtcttttatt ttttgtgtcg cccatgttgc actgtactgg ttacctgcaa caagatcata
     4921 ttgtatcaga cgataatctt ctagaatttc tttttgtttc tcagttaaat ttttttcttg
     4981 gataatttga ctatcctctg taacaaagac ctgttttcca ttaaaacgat tagacaagct
     5041 ttctgtagga tcaattgtta atgcaggtag atgattagat acttctgtca acaacgcata
     5101 gtaaggagtt actttgatat tcgcatgctc aagagccata gcaccaaagt tattaggagc
     5161 aacaacttct ttatttaacg tagctgcttg ttcacgacta tacttgttgt tataaatgaa
     5221 ataatctgtc tggtgctgtt ctaatccata ttttttcgaa tcattcccac tatagattgc
     5281 gggtaaatgg tcaccataaa aaacaaaggt aatcggttta tcgattttgt cgatttcttg
     5341 gatgaatttt tttactgctg tatcggtata gtgaaggcct tgcatataag tttgtaattc
     5401 ttttcttcta ttaggaatga cagcagttcc ttcagcagtg tagttcaatt cactatagta
     5461 atcatcataa ggcatatggt tttgcatagt ggataattgg atgaattgtg acgtttccgt
     5521 attgctttgt aaagctttca aggtttcttt gtatgctgaa tcatccgata tgcgtgggct
     5581 ttgttctatt ttatccgtat aagttaattt atccggactg tctaaataat agaacctgtc
     5641 aaaaccaaat ttatcaaaca cttgttttct gcgataaaga gcagctgtaa aaggatggat
     5701 ggctattttt tcatcgaata gatcggtaat atttggactt attttctgac gctccactag
     5761 ctgggtataa ggtgtaacta acgaagcaga caggttactg atactcaagc ctgtcagtcc
     5821 ttcccattcc atgttagctg ttccgccacc ataaccgacg gataacatca atcctcctgt
     5881 tccttctgtt ataatgcttt tgatgtaagg aatagggtct gtaggtactg tcagattagg
     5941 gacacgtgca ggatcactga agctttcact taaattgaga acgatcgtct gattgtttaa
     6001 ccagtcactt cttgtttggt tgatttcatc tgcctttgtg ttgtactttt tcattattcc
     6061 attaattttt tctttcgaat agccagcagg ttcttccatg attttaatgt cgatattgtt
     6121 taggaattga atgactggtc cgttatcttt tacggcgcct tgctggttaa agaaagtctt
     6181 gtttacttta aataaattaa acagcaagaa agaaggcgaa tttttgtgat taatgaaaaa
     6241 gacacccgaa aaacatccta ataatacagc gatactgata aatcgacgtt tccaatgaat
     6301 cttcaaggaa tactgttttt gtagtctacg ttgtacaata atactagtaa tcaataatag
     6361 gagaaggatc aatccgccca cgattaataa gactgggcta accatcgata atagttctga
     6421 caatccggtc agcattttta aatctgaagg aaagacaggc tcttcccgca tttccatttt
     6481 gattactgtc gaaatagtga gcagtaagtt aatgatcaag gtagcagtca aggcgtacca
     6541 aaaacgattg gaaatcaaca atagtaagaa gaaaaatccc ataataatga tgattgtcag
     6601 gatcatgggt gcttgtcgct gagtaaatat ttttaaggca acctggatag tttctttcca
     6661 accttctttt ggttctaaaa cgaaaatctg cacgaaggta aagcagtaaa aaaatgctgc
     6721 aactaaaaaa taacttttat gaacaaaaag ccaatgaatt ctttttttcc aacgctgagt
     6781 taattgttca atcgaatcaa aagcgaagta ttcgatgaaa cgatgaatga aacggatttt
     6841 ttgtatataa aataaaaaga taactaggag ggacactgct aataaatatc aaataaaaaa
     6901 ctgcccgata tttatcagcc atgccttccc tgagtttgga aattcacgtt tcataaatgt
     6961 agatacgcga taagaaacta aagggaaatt acatataact tgagtgacaa tcaaaaatac
     7021 agcaagaaaa tttcctcttc cttttatacc taatttatca gatagcattt ctaaaaataa
     7081 aaacgaagag aaagtataat acattgcgaa gagagaatat ggtttagaaa acctcagcgc
     7141 tgtatctgta tttcctctga tgatgagtga aatttgtgtc atcaaaataa tcaaaccgaa
     7201 taaaacaatc aagctgataa atacttgagg aagcttatag cggacctttt ctttccagtt
     7261 ccattctttc aaaccataac ccattaaaaa taaataaaaa atccataaaa tactctgtcc
     7321 attctgtaat gcaaacgggt ctttattaaa tagagtagag cagccaacta atccaattgt
     7381 taaaaataat agtagatatt taattgttgt tttcgataat ttatttaaaa atccaactgt
     7441 ataaggaata ctacaaaaaa gcaaagtaca agaaacagca taagtaaagt aattctgact
     7501 gataggtgtt aggataagcc aataatcacg aatattcaag ctgccagctt gtacgtaaaa
     7561 aaagatattg caaactagtg tggctattag gaatataaag gcaaaacgac ttaaataatg
     7621 taaaactttt aaacgggcga ttttttgata ttggtaaccc atgtaaagtg ggatcaaatt
     7681 tgcaccaata acaacggaag tttgtaaaaa tgtatagatg tagaatcgac cagggtttgt
     7741 tgcatatgta ggaatagtga ctgctgaaat caatatacgc tgtaaaacca tcattataca
     7801 agcgacaaag attgtcagga agccataact ctccttcttt atttttttca agaataaaca
     7861 cctcacaaaa aaattggaca aaaacataag aaaacgtttc ctgataagtt gcccattaat
     7921 aacatcctaa cttagaatat ctcaaaaaac aacgtgtaga cagaaaaagt acaaaattag
     7981 caagaaatgg ttgtttgtat tgttagaatt tttagaaaat tataccttta gtttctttgc
     8041 ataaaatgat acaatgattg ttaaaattgt gaaagggtgg tcatttgtta gagaagtttt
     8101 tggatatgtc gttagttcaa cgtattgttg ttggtatttt agtcggaata ttgtttggta
     8161 tctttttccc cacatggaca tttatttcaa tattgggaac gttgtttgtc agttgcttga
     8221 aggcaatcgc acctattctg gtttttttct tgacgatggc gtcaattgcc aagcataaaa
     8281 ttgggaataa gacattcgtg aagcctattt tgatcttata tttagtggga acattgcttt
     8341 ctgcacttgt agcagttatt gtaagttcaa tttttactgt tcctattact ttacaagaaa
     8401 cagtatctga aaaagcaccg caaagtttgg aaagtgtttt atctactatg ttgacgaatg
     8461 tcacacaaaa tcctgttcaa tctcttattg atgcgaatta ccttggcgtc ttattctggg
     8521 ctgtattgtt gggactagct tttcgagcac gttcagaatc gacaaaagag ttggtggatc
     8581 agatatctat agcgttgtca caagtcgtac aagtgattat tgcatttgct ccgataggga
     8641 ttttaggatt ggtttatcaa tcaattgcta cgactggaat cgctggattg gcagagtatc
     8701 tgcagctttt acttgttttg atcggtacga tgctgtttgt cgcattagtt gtgtacccat
     8761 tcctcacgtt tttgtttatt aaagaaaatc cctatccatt gattttcttt tgtttgaaag
     8821 aaagcgcact tcccgctttc tttacccgaa gttctgccgc aaatatcccg ataaacatga
     8881 tgttggctga aagattgaaa ttgacaaaag agtcttattc catatcgatc ccgttaggtg
     8941 ctacgatcaa tatgggcggt gccgcgatta ccatttcctt gatgacgttg actgctgtac
     9001 attctttagg gattgaagca ccttttgttt tgaaagtcct tttatgtatt ttgtctgctt
     9061 tagctgcttg tggagcttca ggtgttgcgg gcggttcact gttattgatt ccattagcat
     9121 gtagtttgtt tggtatctct aatgatgtcg cgatgcaaat cgtcggtatt ggatttatta
     9181 tcggtgttat ccaggattca atggaaactg ccttaaattc gtcaagtgat ctgttgttta
     9241 ctgcaatagg agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa ttgaaagatt
     9301 gtttagatgg attaagtgaa cgttgaacgt tagatattta gtatttttaa aatgattcga
     9361 gtttttaatg agttatatag atgttcgtgt aaatagaggt ttctgttaaa tcaagataaa
     9421 gtcgtttata ataacaatta aaagcatagg taattgttat tggaggcgta tcatggattt
     9481 tttacgtaaa tttggttttt tctttttatt attaggcgtg ctgagtgatt ttttgactcc
     9541 gtacgtatta ggattgtatt atcctgaatt agatcaaatg aagagcgtga tcagtgtatt
     9601 tggagaggtt tccagtcctg taagaaaagc gtttcttatt tggtctgtgg tttcagggat
     9661 ctttttcatc ctgtcattgc ctgcattgta tgctgcattt gttagtacat ctcgtgtgtt
     9721 agctacccta gttacagcag ccatcgcgtt ctatggtata ggtgattgta tctttacagg
     9781 tttgttcagt atcaataccc atgaatcttc gtggaatgtc tcgacatggg tccataatat
     9841 cggatcaggt ttaggttaca gtggtttttt attgtttcca gttcttcttt tcttgatcta
     9901 tcgaaaaaat gggcaaatgg acgtaagcca cttatacctt ggactgtctg tgatcagttt
     9961 gctttttgcg gcgatttatg gactggcaag aattccttct ataaattctt ggccattact
    10021 gaataaaata ggtttttgcc aaagaatcag ttttttcttt aattatgtgc ccattgcctg
    10081 gttgagtatc ttgcatctca aacagtaaaa cttgagccgt caaattgaac agtcagcgga
    10141 tacttcatcc ggtggctgtt ttttgttttt ctgaaatata tatttccaca gttttcttgt
    10201 atagcttctg tcttagagaa gcaaggagct atattttgtg ctacaatctt aaaaaggtag
    10261 agagaagacc aaatggaaga cagaggagcc actaaaatat gagaaacatc aagttgacaa
    10321 tcgaatatga cggaaaaaga tattcaggat ggcaaagact tggagatgac gataaaacca
    10381 ttcaaggaaa aatcgaaaaa gtattgcatc agatgacaaa tgaagagata gaaattattg
    10441 gatcaggaag aacagatgct ggcacccatg cgcgaggaca agtagcaaat tttaaaacaa
    10501 acacagaaat tggactggca gaaatgatcg actttatgaa tcgttacctt ccgcgggata
    10561 ttgtcatcaa aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat aatgcggtgg
    10621 gcaaaaaata cagctattat gtttggaaca acgtgattcc ttcagcattt gagcgaaacc
    10681 acagctttta tttccctcaa gagttggata tggacaagct gaatgcagct tgcgaaaaat
    10741 tagtgggtac acatgatttc attggttttt ctgcattgaa aaaatcaaaa aaatccacag
    10801 tgcgtacgat tgatgaatta tctatcgaac gaaagggaga aatgcttcat tttacttttg
    10861 tcggagacgg attcttatat aaaatggtgc ggattatcat ggggacatta ctagaaatcg
    10921 gcacaggtgc catgccgata gaaacaatcg atgaaatatt tgaaagcaaa gtgcggcaac
    10981 atgcaggaga aaccgtccct gcacaaggtc tgtttttgga tgaagtttat tatcgataaa
    11041 aatactttga taatggatga aacgcatgtt tcacagtata aatatcgatg ttaaataatg
    11101 tatgaagcaa attaaagaaa tataaaaaaa gactttttag cctgtttatg tgtacagcta
    11161 aaaagtcttt cttaaataaa aatctagtga gaatgttgta cttctcttct cggttgtttt
    11221 tgtttagaaa aagtaaatcg ataaagtaaa aataaaatca aagtaacgat agataaatat
    11281 cttgaagcaa ttgctagttt tgtgtactga taagtaataa cgactttatt tatacctgga
    11341 ggaatagtta attcagttgt gccaaactta gataattttg tttgtactag tttgccatta
    11401 atagaagcca cttgcccttt ataacgataa ataggtgtcg tcaagacagt atttttatta
    11461 ttcgaattat ctaattcaat tgtaagtttg ttactcataa aatgagttgt tggatttatt
    11521 tcctggttac ctagtaaata ttgatcatta ttaatcatat caggatgata gattgactct
    11581 ttgtttgcat aatcagtatg attgtaattt tcagctatag caacagcgtc ttcaggtgct
    11641 ataagtgtta atgaatcttt gttagcatgg atagaattgc ttactcctga gagatgcaat
    11701 attagtgcaa gaaacgtaaa gaccattatt ttccatgaat tatttttagt agaaaaaata
    11761 taatagatca ctatactcat cgtataggct ataaataaag aagaaaaacc atttaaccgc
    11821 cagacaaatt gtagactctt aactggtgta tggttaaaaa gttgccaagg aaataaattg
    11881 gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa ataaatcatc agcagttaat
    11941 ttttttataa aaataaaagc accaatcaat ccgagaaata agaataatcc aatagtatag
    12001 ttgttatagt tattatttaa aatatgtgtc agcatatcac taggagccat accacttaat
    12061 tcttttccca gtgggacttt caattcttgt gctctagatt gttgaataaa aggaattaag
    12121 aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa gagtctagcg
    12181 attttttggt cccaaaaata aaaagaaaga aacaacgttc ctccaataaa tacagaaacc
    12241 atagcgacag aaagtaaatg agtatacact actagcgtca tgccaataga caaccagtac
    12301 catttttggt aatctctaat ataaatttcg taacacccca ttagtatcaa aggcaaaaat
    12361 gttaaagcga cagcttctcc caatgaagca cgatggaata tgtcaatggc acgataggcg
    12421 gaaaatgtat aaataatcga aaataacagc gaaatataac gattgttttt tatttgtttc
    12481 atagaaaaat aagaaacgac cattgttaaa aaagtgataa aaaaatagta gatattataa
    12541 cttaaaacaa gatttcccat cattttataa aataaaaaag ctggataaag agtaagccat
    12601 ggataaaaaa tattcatcat tgttccatga tgatcgaagt tattgaagtt tactggagaa
    12661 ctccatacat tatctagtcc tatgattcga gataaatgaa agtaagtatc ctgattgaca
    12721 ctatgataga ttcccttaga catgaataga gggatgacgt aagcaaaact tagaaataac
    12781 ata
//
LOCUS       DBKOIJ010000073        12752 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000073 DBKOIJ010000000
VERSION     DBKOIJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.075"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..730
                     /locus_tag="ACV5NJ_001627"
     CDS             <1..730
                     /locus_tag="ACV5NJ_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HHY6485715.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            788..2827
                     /locus_tag="ACV5NJ_001628"
     CDS             788..2827
                     /locus_tag="ACV5NJ_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HHY6485716.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2936..4087)
                     /locus_tag="ACV5NJ_001629"
     CDS             complement(2936..4087)
                     /locus_tag="ACV5NJ_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HHY6485717.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4241..4771)
                     /locus_tag="ACV5NJ_001630"
     CDS             complement(4241..4771)
                     /locus_tag="ACV5NJ_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HHY6485718.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4870..5220)
                     /locus_tag="ACV5NJ_001631"
     CDS             complement(4870..5220)
                     /locus_tag="ACV5NJ_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HHY6485719.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACV5NJ_001632"
     CDS             complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACV5NJ_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HHY6485720.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACV5NJ_001633"
     CDS             complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACV5NJ_001633"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HHY6485721.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7623..8285)
                     /locus_tag="ACV5NJ_001634"
     CDS             complement(7623..8285)
                     /locus_tag="ACV5NJ_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6485722.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8469..10115
                     /locus_tag="ACV5NJ_001635"
     CDS             8469..10115
                     /locus_tag="ACV5NJ_001635"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HHY6485723.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10106..11188
                     /gene="uxuA"
                     /locus_tag="ACV5NJ_001636"
     CDS             10106..11188
                     /gene="uxuA"
                     /locus_tag="ACV5NJ_001636"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HHY6485724.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11296..>12752)
                     /locus_tag="ACV5NJ_001637"
     CDS             complement(11296..>12752)
                     /locus_tag="ACV5NJ_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6485725.1"
                     /translation="TTQYLADHFHMSESNFLLYIKELEQDFERLNLTELHIDKQKPFL
                     KLNFEGIDPAYCYYRLFGRYCNESVSYQILTSLFSCQTNSIISLSQQTNYSASYLYTK
                     MKKINAFLALYGLSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQV
                     VAFMMNTFFKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFI
                     DPSVDILRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSRE
                     MVESFCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKE
                     VQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRD
                     FYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITP
                     EDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         4267 a         2349 c         2365 g         3771 t
ORIGIN      
        1 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
       61 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
      121 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
      181 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
      241 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
      301 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
      361 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
      421 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
      481 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
      541 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
      601 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
      661 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
      721 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
      781 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
      841 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
      901 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
      961 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     1021 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     1081 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     1141 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1201 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1261 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1321 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1381 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1441 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1501 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1561 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1621 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1681 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1741 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1801 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     1861 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     1921 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     1981 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2041 tgctcctgga tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat
     2101 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2161 atcttttcaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2221 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2281 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2341 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2401 atatggacag ggcgaacttc taattaatcc tatccagcaa gcagcaatgt attctgtttt
     2461 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2521 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     2581 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2641 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2701 gtttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2761 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2821 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     2881 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     2941 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3001 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3061 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3121 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3181 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3241 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3301 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3361 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3421 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3481 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3541 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3601 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3661 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3721 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3781 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3841 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     3901 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     3961 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4021 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4081 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4141 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4201 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4261 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4321 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4381 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4441 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4501 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4561 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4621 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4681 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4741 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4801 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4861 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     4921 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     4981 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5041 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5101 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5161 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5221 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5281 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5341 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5401 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5461 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5521 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5581 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5641 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5701 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5761 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5821 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     5881 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     5941 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6001 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6061 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6121 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6181 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6241 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6301 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6361 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6421 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6481 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6541 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6601 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6661 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6721 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6781 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6841 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     6901 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     6961 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7021 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7081 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7141 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7201 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7261 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7321 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7381 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7441 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7501 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7561 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7621 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7681 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7741 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7801 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7861 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     7921 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     7981 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8041 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8101 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8161 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8221 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8281 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8341 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
     8401 atagcgattt ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga
     8461 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
     8521 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
     8581 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
     8641 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
     8701 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
     8761 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
     8821 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
     8881 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
     8941 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
     9001 ttcttgctgc gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
     9061 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
     9121 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
     9181 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
     9241 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
     9301 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
     9361 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
     9421 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
     9481 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
     9541 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
     9601 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
     9661 caaatattcc tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
     9721 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
     9781 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
     9841 gaaaaccatt catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg
     9901 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
     9961 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    10021 aaatgttcat caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact
    10081 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    10141 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    10201 attgatggat ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga
    10261 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    10321 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    10381 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    10441 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    10501 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    10561 atttgaattg ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc
    10621 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    10681 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    10741 ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg
    10801 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    10861 agaaaatgat gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc
    10921 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc
    10981 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    11041 cgaatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    11101 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    11161 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    11221 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    11281 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    11341 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    11401 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    11461 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    11521 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    11581 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    11641 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    11701 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac
    11761 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    11821 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    11881 aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac
    11941 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    12001 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    12061 tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata
    12121 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    12181 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    12241 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    12301 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    12361 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc
    12421 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    12481 ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    12541 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    12601 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    12661 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
    12721 atggaaatga tctgctaaat attgtgtagt aa
//
LOCUS       DBKOIJ010000074        12742 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000074 DBKOIJ010000000
VERSION     DBKOIJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.076"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACV5NJ_001638"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACV5NJ_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HHY6485726.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACV5NJ_001639"
     CDS             complement(323..892)
                     /locus_tag="ACV5NJ_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HHY6485727.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACV5NJ_001640"
     CDS             1078..1980
                     /locus_tag="ACV5NJ_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HHY6485728.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACV5NJ_001641"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACV5NJ_001641"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HHY6485729.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACV5NJ_001642"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACV5NJ_001642"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HHY6485730.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACV5NJ_001643"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACV5NJ_001643"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HHY6485731.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACV5NJ_001644"
     CDS             8554..8739
                     /locus_tag="ACV5NJ_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HHY6485732.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACV5NJ_001645"
     CDS             complement(8801..9997)
                     /locus_tag="ACV5NJ_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HHY6485733.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACV5NJ_001646"
     CDS             complement(10167..11603)
                     /locus_tag="ACV5NJ_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HHY6485734.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACV5NJ_001647"
     CDS             11896..12705
                     /locus_tag="ACV5NJ_001647"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHY6485735.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4013 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aa
//
LOCUS       DBKOIJ010000075        12568 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000075 DBKOIJ010000000
VERSION     DBKOIJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.077"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            26..763
                     /locus_tag="ACV5NJ_001648"
     CDS             26..763
                     /locus_tag="ACV5NJ_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHY6485736.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            777..1592
                     /locus_tag="ACV5NJ_001649"
     CDS             777..1592
                     /locus_tag="ACV5NJ_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HHY6485737.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1605..2252
                     /locus_tag="ACV5NJ_001650"
     CDS             1605..2252
                     /locus_tag="ACV5NJ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHY6485738.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2268..2924
                     /locus_tag="ACV5NJ_001651"
     CDS             2268..2924
                     /locus_tag="ACV5NJ_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHY6485739.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3086..3907
                     /gene="racE"
                     /locus_tag="ACV5NJ_001652"
     CDS             3086..3907
                     /gene="racE"
                     /locus_tag="ACV5NJ_001652"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HHY6485740.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3910..5265
                     /gene="rph"
                     /locus_tag="ACV5NJ_001653"
     CDS             3910..5265
                     /gene="rph"
                     /locus_tag="ACV5NJ_001653"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HHY6485741.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5266..5784
                     /locus_tag="ACV5NJ_001654"
     CDS             5266..5784
                     /locus_tag="ACV5NJ_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HHY6485742.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5891..6391
                     /gene="cbpB"
                     /locus_tag="ACV5NJ_001655"
     CDS             5891..6391
                     /gene="cbpB"
                     /locus_tag="ACV5NJ_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HHY6485743.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6671..7591)
                     /locus_tag="ACV5NJ_001656"
     CDS             complement(6671..7591)
                     /locus_tag="ACV5NJ_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HHY6485744.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7717..8769
                     /locus_tag="ACV5NJ_001657"
     CDS             7717..8769
                     /locus_tag="ACV5NJ_001657"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HHY6485745.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(8879..9634)
                     /gene="fabI"
                     /locus_tag="ACV5NJ_001658"
     CDS             complement(8879..9634)
                     /gene="fabI"
                     /locus_tag="ACV5NJ_001658"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HHY6485746.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9854..10294
                     /gene="fabZ"
                     /locus_tag="ACV5NJ_001659"
     CDS             9854..10294
                     /gene="fabZ"
                     /locus_tag="ACV5NJ_001659"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HHY6485747.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10409..11170)
                     /locus_tag="ACV5NJ_001660"
     CDS             complement(10409..11170)
                     /locus_tag="ACV5NJ_001660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHY6485748.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11359..12453
                     /locus_tag="ACV5NJ_001661"
     CDS             11359..12453
                     /locus_tag="ACV5NJ_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HHY6485749.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4050 a         2177 c         2648 g         3693 t
ORIGIN      
        1 taaataataa tgggagtgta attctatggc aatgattaaa tttgatcacg tggataaata
       61 ttatggcaag ttccacgcat taaaaaatat caatctcgaa ttcgagaaag gagaagttgt
      121 cgttgtcatt ggtccttctg gttcaggaaa gagcacgatg cttcgctgta tcaatggatt
      181 agagacgatc tcttcaggta agctgctgat taatgatact gacttgcatg ataaaaagac
      241 aaaactgaca gaagtccgaa aaaacatcgg catggttttc caacatttca atttgtaccc
      301 aaataaaact gttctggaaa acatcaaatt agcaccaatc aaagtgttga agcaagacca
      361 ggcaactgca gtcaaaaacg cggagaaatt tttgaaaact gtcaatatgt tagataagaa
      421 agattcttat ccttccatgt tatccggggg acaacagcaa cgggtggcaa tcgccagagg
      481 tttagcgatg aatcctgaga tgctgctgtt tgatgaacct acttctgctt tggatccgga
      541 aatgattggt gatgtattag acgttatgaa aaaattggct catgacggta tgtcaatgat
      601 tgtcgtgaca catgaaatgg gctttgccaa agaagtggcg gatcgtgtta tcttcatggc
      661 tgatggagaa gtattggaag acagcagaaa tgtgagagac tttttcgaaa atccaaatga
      721 agtccgagca cagcaattta tcagtaaagt tatcaatcat tgataggagg aaaaagatgc
      781 gcaggttaaa aaaaatacgc ctgattttcc ttttaggcat tgcttttctt gcactagccg
      841 gatgtaaagg aaatgggttg gcaaatgaag atattttaga gcgaagcaaa tctaccaatg
      901 aaatcatctg gggtgtcaag tatgatactc gattatttgg aatgatggat attgagagca
      961 gaactgtaca aggatttgat gtagatattg caaaagcaat cactaaaaag atccttggtg
     1021 acaatggaaa aacagaattt gttgaagtca cttctaaaac tcggattcca ttattgaaaa
     1081 acggaaatat cgatgcaatt attgcaacga tgacgatcac agatgaacga aaaaaacagg
     1141 ttgacttttc agatgtctat ttcgatgctg gccaagcttt gttggtaaag aaaggaagcc
     1201 aaatcaaaag tgtagatgat ttgaatgctt ctacaacggt tctagcagtc aaaggttcta
     1261 catcggctgc gaatatccga cagcatgcac cagatgcaaa aattttagaa ttagaaaact
     1321 atgcagaagc atttactgct ttacaatctg gtcagggaga cgcgatgact acggataatg
     1381 cgattttgtt agggattgca gatgaaaatc cagagtatga gttagttggt ggaacgttta
     1441 ctaatgagcc ttacgggatt gcgatcaaca aagggcaaga aaacttcttg aaagctgtta
     1501 accaagcgct agaggaaatg catgcagatg gcacatacga taaaatctat caaaaatggt
     1561 ttccaaatga aactgaagga aaagtagagt aagggggaga ggcaatgagc gaattgatac
     1621 aaacttatgg accaatcttt gtcgacgggt tcaagatcac attgttttcg agtgtcttag
     1681 cacttgtgtt tagtttgatt atcggaacgt taatggcaat cttccaatta tcaaagaata
     1741 aagtagtgag cgccttagca aaggcatatg tagaattttt tagaaacatt ccattattaa
     1801 tcatcgtgat gtttttttat gttgtcgtac cgatttattg gtttaatcta gatggattcc
     1861 aagcaggtac gattggatta acgatctata catctgcttt tattgctgaa acagtacgtt
     1921 cagggattca aaccgtacca aaaggtcaaa cagaagcagg actatcttct ggttttacct
     1981 attcagaaat catgcgttat attgttttac cgcaagcatt caaaatcgtt atcccgccat
     2041 taggcaacca atttatcaac ctcgtaaaaa actcatctat tttagcaatc gtagctggtt
     2101 tagatttgat gtatcaagga gacttgatcg ctagtgaaac attcaatacc ttcgatacgt
     2161 atatcatcgt tgggttattt tacttagtta tcacgctacc gttatcttac ttaatggttt
     2221 atcttgagaa aaaatgggcg gttcgttcat agaaggaggg gaaatacatg gattttagcg
     2281 gcgctttttc atggatgaat atccgttttt tattggaagg attaaaggtt actgttgaag
     2341 tttcattgtt ttcgatcatt tttagtttct tgatcggtgg tctgacaggt gtgattcgtt
     2401 ttgcgaatat ccctttcttt tccaaaatcc ttggtgtagt gattgatatt atacggaatc
     2461 ttccattatt attgatcatt ttcttcactt actttgcctt acctcaaatc ggcattcaga
     2521 tgaacatctt ctggtccgct gtcgcagcat taacgatttt tgagtcagct atgttgtcgg
     2581 aaattttccg agctggactc aatgctgttc caaaaggaca gatggaagca ggattatcaa
     2641 cgggactgac ttatgtagaa tcgatgcgaa cgatcgtgtt accgcaagca tttaaatcaa
     2701 tgatcccggc aattgtgagc caattgattt cactgatcaa agatacttca ttagcagtaa
     2761 tcatttcttt acctgaactt tctcatcagg cacgtattat ttatggacag aacaccaatt
     2821 atgtgcttcc gatgttcatc atcatgacac ttatgtactt cgtggtatgt tacgcgcttt
     2881 ctctgctttc cggttacttg gaaaagagac ggtacagtta ttaatagatg attcttttag
     2941 cggttgtggc aaactaaagt cacaaccgct tttcctcttt ccattcgaaa aaaagagatt
     3001 tcgattgttt taagcaagta agagcacttg aaaattcacg ctaaaaagta cacaataggg
     3061 aggatagaat ttattgaaaa aaaggatgat acgattgaca gataatcgcc ctatcggatt
     3121 tattgattca ggtgtcggcg gcttgactgt agtaaaagaa gccctgaaac aattaccgaa
     3181 tgaaaatatt ttatttgtag gagacacagc acgctgccca tatggcccta gacccgcgga
     3241 acaggtaatc cagtatactt gggaaatgac ggattatctg gtggagcaag gaatcaagat
     3301 gctggtgatc gcctgcaata ccgcaactgc ggtggcttta gaagaaatca aagctgctct
     3361 ttctattcca gtcatcggtg tgatccttcc cggtactaga gcggcagtaa aaaaaacaca
     3421 aaataaacaa gttggcatta tcggtacgat tggtacggta aaaagtcaag cttatgaaaa
     3481 agcactgaaa gagaaagtac cagaattgac tgtgacaagt cttgcttgtc ccaaatttgt
     3541 ttcagttgtc gaaagtaatg aataccattc atcggtggcg aaaaaaattg tggcagaaac
     3601 attagctcct ttaaccacta aaaaaatcga tacattgatt ttgggatgca cccattatcc
     3661 attattacgc cccatcattc aaaatgtaat gggagaaaat gttcaactga tcgattctgg
     3721 agcagaaaca gtaggtgaag tatctatgct gttagattat ttcaatttga gcaattcacc
     3781 gcaaaatggt cggacattat gccagtttta tacaactggc tctgccaaac ttttcgagga
     3841 aatagctgaa gactggcttg gaatcggaca cttaaatgta gaacatatcg aattgggagg
     3901 aaaataagga tgcgtcatga cgggagaaaa gcccaagagc ttcgcaaact ggagcttcaa
     3961 acaaatgttt tcaaatatcc agaaggatcg gtagtgatcc gttttggtga tacaacagtt
     4021 atttgttctg caacaataga aaattctgtt cccccttttt tgagagaaac aggaacagga
     4081 tgggtgacag cagaatatag tatgctgcct cgagcaacga gtacgagaaa tcgtagagaa
     4141 agcagcaaag ggaaactttc tggtagaaca atggaaatcc agcgtcttat tggtcgttcc
     4201 ttgcgtgcag tagtagactt ggaaaaatta ggagaacgta gtatcatcgt agactgcgat
     4261 gtgatccaag cggatggcgg tactagaaca gcaagtatca ctggggcatt tgtggcattg
     4321 aaattagcca ttgaaaaact tttaagagaa aaagaattgt cagaagatcc aataaaagaa
     4381 catcttgctg cagttagcgt aggaatctta ccagatggga cttgcgtcac agatttggat
     4441 taccaagaag attccgcagc acttgtagac atgaatcttg taatgacaga atctggaaaa
     4501 ttcgtggaga ttcaaggaac tggagaagaa gcaacgtttg atggtgaaca gttgaatgaa
     4561 atgctctttt ttggaaaaaa cgcgattgaa gatttgatca aagagcaaaa acatgctttg
     4621 ctaacagagt ttgctcaaaa tgatgagcgg atagaagaaa caaaaacaat catcattgcg
     4681 acaagaaatc caggaaaagc agaagagttt cgaaatatgt tcaaagaagc gggatatcat
     4741 gtaaaaacat tacttgacta tcctgaattg ccagatgtgg aagaaactgg cagcacattt
     4801 gaagaaaatg cccgattaaa agcagaaacg attgcacaat tgcttgatca gccagtctta
     4861 gcagatgatt caggtttgaa agtggatgct ttaggcggta tgccaggaat ctattctgca
     4921 cgttttgcag gggaacaaaa aagtgatgcg gggaataacg ccaaattatt atatgaattg
     4981 acagatgtgc cggatgaaaa aagaacagca caattccatt gtacgttagt attcgcggca
     5041 cccaaaaaag acagccttgt agtagaagcg gaatggcccg gtcgagtagc ccgtatacca
     5101 tctggcgaaa atggctttgg gtatgatcct ttatttatcc cagaaggtaa gaaacaaaca
     5161 gcagcagaat tatcgagcga agaaaaaaac aagatcagtc atcgggctca agcaatgaaa
     5221 aaattgagtg ccgagtggaa acaatggctg gaaggagaga gatagatgcg gtatttagta
     5281 gtaagtgata atcatggaga cagagatatc ctcgttgact tagtagatcg ttatcgttct
     5341 gaagtggata tctttttcca ttgtggtgat tcggaattag aagcttctga cccattatgg
     5401 gatacattcc aagtggttca aggtaattgt gattatggac caggattcga acagaaaaaa
     5461 gtgatccaaa ctggtcagga tacagtattc atgactcacg gtcatttatc gaatgtccga
     5521 tttggtttga cacagttagc aatcgaagct cgagctgcaa ctgccgatat ggtgtttttt
     5581 ggtcataccc atcaaatagg ctgtgagatg gatcagaaaa tcctgttttt gaatcctggt
     5641 agcatttctc agcctagagg cccgatccaa atcccatcat atgctcttat cgaaagttta
     5701 gatgaccaaa tttctgttca atactataac cgtgcccatc aggcagttga agagttggcc
     5761 ttttcttttt cgagagagcg ctgaataaaa aaactctaat ttttaataaa aataagcttt
     5821 attttatgaa ttttgcatcc ggtttcggta aaatagtagt gttgaatgaa atagaaatgg
     5881 aggccatcag atgattggac ctatagtaag agaaatgctg ctggaaaatc aggaaacgtt
     5941 tttgattcct gcagaaaatg tggcaaatgt tatgtatcat catccattgt cccatggttt
     6001 acttgtgtta tcgaaggtcg gttattccaa aattcctgta ttaggaaaag atgatcactt
     6061 tgtaggacta gtaagtttat cagatgtggt caataaaatg attgacttgc aaacaatcag
     6121 catggacccg ttagaaggac taacggttgc tgatgtgaca gaaacagatg ttccgacaat
     6181 tgatgaaaat tgggaactag aagaagtact ccatttgtta gtcgattcgc cttttttacc
     6241 ggttgtcaca gaagatcgga ttttcaaagg aatcattaca agaaaagagc tgcttaaagc
     6301 cgtaaacttc atggtgcatg aattagaacg tcgtaatata ttgtctccga aaatcgaaga
     6361 attaaaagaa caaatcgata tcgtgagcta aagaacgaga aactaaaaga taatcaaaaa
     6421 ataagtagaa aaaacagcgt accagttttg actttatctt gaaaaagagg ctgggacaaa
     6481 agcgtttaac tccaagaaat aagaaggaat tcacgaaaat tgcttttcaa atttttgtga
     6541 atttcagctt atttccgaag gagttgcttc tgcttccgcc gtttatacgt gtttagagcg
     6601 tgggacaaaa gtgttcttta cttttgtccc acgctctttt tagtgagatt cgttgcatcg
     6661 tttgtccaaa ttattttccg acagcaattg gactgttcgg aatcgtgaaa ccagaagctg
     6721 tcaattcttt gacataggag ctcaagaact cttcttttaa ggcatattgt tttccgttca
     6781 atgcgtagca gaccgtacga atcgcaaaat tgctgtttcc aagatccact aatccaaaga
     6841 tagaaggttc agtttggagc tcttcttggt atttttcagc taatgtacga tttactcgat
     6901 caatgatctc atagattttg tcatatcctt cttcaggaac aatccgaata tctaaaagta
     6961 cttgcatatt tgcacgagag agattgctga tcgtggtgat gtttcgattc gggatgaaat
     7021 ggacagtacc atccgttgct ttcagctgaa gggtacggat accaacagat acgacggttc
     7081 cttcaatcgt taagtttgct agcttgatgt agtccccaac atccatctgt tgttccatga
     7141 taataaaaaa tccggtaata acatcactca tgaacccttg ggcaccaaga ccgattgcca
     7201 cacctgcaat tccggcacca gccaaaagtg atccgatcgg cacaccgata gcagataaaa
     7261 ttgcatagat aaaaaagaaa ccaagggtat aatgaaaaat agtatttaaa agactatgta
     7321 aagtctttaa gcggctctcg tttagaatgg cttttttaga atactgatta aaagacttat
     7381 caatcagata ttttcccaca cggtgaagaa tccaaaaaag gacgatcaaa aaaatcagat
     7441 ataaactttt ttggataaag gcagccaaaa tggcgtccca atcaatcccg tcccaaaacc
     7501 gctgaaacgc atttactttg ctgacagctt gatctgtcaa tgtctcgcca gaagagtctg
     7561 tcgtaccggc agttgctaat aatgaaaaca taaaatcctc ttttctaaaa tttgattctt
     7621 tctgtattgt accaagaagt tttcaaaaaa ggaaatagca aaaataacta taatgaagga
     7681 aaaaagctga aaataaaatg acaaataata tggataatga taaaatgtgg agaaaataac
     7741 gaaggtggta agtatatgca aatcgattgg aacaatctag gtttttctta tatcaaaact
     7801 ccttggcgtt ttattgccaa atggaaagac ggacaatggg aagaaggaga attgacagaa
     7861 gataattacc tgacgattca tgaaggctct ccagcgcttc attatggtca gcaatgtttt
     7921 gaaggtctaa aagcttatcg tcgaaaagac ggcaaggtca atctctttcg acctgaacaa
     7981 aatagtcgac ggatgaatca aagtgctaga cgattgctga tgccaatggt ccctgaaaag
     8041 atgttcgttg atgctgtcaa gcaagtggta aaagcaaatg aagattttgt gccgccctat
     8101 ggaacaggcg ctacattata cttgcgccct ttactgattg gggtcggtga aaacattggt
     8161 gtccatccag ctccagaata tttatttgtc gtcttctgta cgccagtagg agcgtatttc
     8221 aaaggtggac taaaaccaac gaattttatt gtatctgatt acgatcgtgc tgctcctaag
     8281 gggactggtg cagcaaaagt cggcggaaat tacgcagcga gtttacttcc aggaacagaa
     8341 gcaaaagaac tggattactc agattgtgtt tatcttgatc cagccacaca tacgaaaatc
     8401 gaagaagtag gtgcagcaaa tttctttgga attacaaaag acggaaaatt catcactccg
     8461 caatcgccct ctatcttacc aagtatcacg aaatattcct tgttgcagtt agctgaagaa
     8521 cgactaggac tgcaagcaat tgaaggagat attttcattg atgatctaga tcaatttgca
     8581 gaagctggtg catgtggtac agcggctgtt atttctccaa tcggtgggat ttatcacaat
     8641 aacaatcttc atgtatttta tagtgaaacg gaagtaggac cagtaacaaa acggttgtat
     8701 gatgaattga caggtattca atttggagac atagaagcac cagaaggctg gattgaagtc
     8761 gtggaataat ttcaaagtaa tgagttgaaa aaaacgtaaa aagaaaaaat caggaagaga
     8821 ctttaaaaag ttttttcctg atttttatat gttttacttt aaagaatgca acaaaagttt
     8881 atgaaagatg gacacctttg tcaacgtaga taatatcacc gatgattccg ctggaaagtg
     8941 ggctgattaa gaaagcacaa gcattaccga cttcttcgat cgtcacacct tgctgatcag
     9001 gcgtacgaga ttcagataac tggatcagtt tttgatagtc tttaacacct gtgacagcta
     9061 atgttttgat tgccccagct gagattccat tgacccgaat tcctttaggc gagaactcag
     9121 ctgccaaata acggatagca gcttcaagag aagctttcgc aatacccatc atgttataat
     9181 tcgggattgc gcgttctgaa cctagataag tcatgctaac gatcccgctg tttggtgcaa
     9241 gaaacggttg tgcgtatttt gatacagcaa ttaaagagta gctgctgata tcttgagcta
     9301 attgatagcc gtctctggaa atatcagaca catttccgga caattcttct ttgttggcgt
     9361 aagcaactgc atggacaagg ccgtggatct ccccagcata ttcaccgatg gcattcattg
     9421 ctttttcgat gctttcgtcg ctagaaacat cacattctac agtgaatgct tcttcgccga
     9481 ccaatttgac taatgatttt ttcattcgtt cattttggta agtatagata atttctgccc
     9541 cttgtttcaa cattgcttca gcacaacccc aagcaatgct ttttttgtta gcgacgccca
     9601 taatgacgat tttttttcct tctagaaatg acataatgac tctcctttgt ttctgataaa
     9661 aatttgatag tcgtaaaaaa atagagaata atagctaaaa taacttcttg atttcttaat
     9721 tattagtcaa aatgttttga ttgtcaaact attttttctt aaattaaaaa aagattgcta
     9781 aatatttagg aatatgatac tttgatagtc aaagtaatga aaatggcaat tgcctgaaag
     9841 aaggagttta attatgtctt acgtattatc tgctactgaa gtaatggaat tgattcctaa
     9901 tcgttatcct atttgttata ttgattatgt agatacttta gaaccaggaa aaaagatcgt
     9961 tgcgacaaaa aatgtaacaa tcaatgaaga ttttttccaa ggtcactttc ctgacaatcc
    10021 agtgatgccg ggtgcgttga ttctagaaac actggcacaa gcaggttcga tacttatcct
    10081 aaaatcagaa gagtttctag gaaaaagagc ttatatcggt gggatcaata aggccaaatt
    10141 ccgtcaaaaa gttgttccag gagatgtgct gaaatgtcat tttgaaatca ccaaaatgaa
    10201 gggatcagtc ggtacagcgt tatctgcagc atatgtagat gataaaaaag tctgtgagtg
    10261 cgagttcacg ttcatcgtca accaaaatgc ttgactataa aacctgatat agcaaaacaa
    10321 aaggaagctt acttgctttt tatgtaaaaa gaagacgcct gaatcctagc atacgttgtc
    10381 atctatgcag gattcagacg ttattttttt atttttgttt tgcttcttgc agtatattga
    10441 ttttatcgat ttttacttct tcttttggtt tgtctgaact atctgtttcg atatttgcaa
    10501 tagcatccac gacatccatt ccttcaatca cttggccgaa gacagtataa tggccatcta
    10561 gtgaaggata accgccgttt ttataagcgt cgatgattgc ttgaggatag tcgtctttta
    10621 aaagaccatc atgcatatca tctgtatttt gaacgataaa gaactggctg ccgttactgt
    10681 taggatcttg tgagcgagcc atagataacg cacctcttat attataaagg tggttgttga
    10741 tctctgtttc aaatccttct ccccaaatgc tttcccctcc agtgccatcg cctttaggat
    10801 cgccaccttg gatcatgaaa tctttgatga cgcgatggaa agtcagtccg ttgtagtaac
    10861 cgtcttttgc atgtgtcata aagttttcaa ccgcttttgg tgcgtgttcg ggaaataatt
    10921 tgattttgat tgtgccttta ttcgtcacca tttcgaccag atcttcattt tcggcgacat
    10981 ctttcgtcaa ttgtggtaat tccaatgcgt tcaaatcgac gctactggca gtcgtgcttt
    11041 ccgcagtaga tgtacttttt gaactttctg tagacgaact agaagaagtc gtgttgtttg
    11101 cagaacaacc agcagcaaac agcaatgctg ctgctaaact aatacttaaa ataactttat
    11161 ttttcttcat aactgttcct ttccaattta ctattttgtt atctttaatg ataacaaaat
    11221 agagggaaaa tacctactta aattattgga atttatcatt tattttaaaa gaaaaagaaa
    11281 aggtgtagac catttacttt gtgtttgcta taatgtaagt ggatacaaaa acgaaaagat
    11341 gcaggaggaa atggtaatat gggaaaatta ggaatctcga tttatccaga acggtcaaca
    11401 tttgaaaagg acaaagcgta cttggattta gctcataagt atgggtataa acgggttttt
    11461 accagtttgc tgcagatcaa tgatgataaa gaaaaagttc tttcagaatt taaagaagta
    11521 gttgattacg cgaatagcct aggcatggaa gtaatggttg atatcaatcc agcgttattt
    11581 gaacagttgg agatttctta cgatgactta tcttttttcc ataagatggg tgcctacggg
    11641 gtccgcttag atatcggatt cacaggagca gaagaagcaa aaatgacgcg caatcctttt
    11701 ggtatcaaaa tcgagatcaa tatgagttcg ggcacgaatt acgttgacaa catcatgagt
    11761 tattcgccaa acacggataa tttattaggt tctcataatt tttatcctca tcgatactct
    11821 ggtttaggat acgaacactt tgttttttgt tcagaaaaat tcagaaaata taatttgaat
    11881 acgatggcat tcgtcaattc gcaatctgct gagtttggcc cttggcctac gcaagatggc
    11941 ttgtgcacgc tggaagacca ccgagatctt gaaattgcga cacaagtgaa gcatttaatc
    12001 cttacaggat tgattgatga tatttctatt ggaaatgcgt acgcaagtga agaagaattg
    12061 aaagaaatgg cagaagcatt taacgcggat tatccaacat tgaaagtaga tacggaagaa
    12121 ggtattacag aaaacgaacg aatctgcttg tttgataacc ttcatagtta tcgaggggat
    12181 cgctcagaat atatccttcg ttcaacgatg actcgtgtat actacaaaga taaagatttc
    12241 ccgcctcata atacgcgaga tatgcatcat ggggatgtac tgattgacaa tgaagggtac
    12301 ggtcaataca aaggagaaac acaaatcgcc ttgaaggata tgaaaaacga cggacgtgtc
    12361 aatgttgtag gaagaatctc cgatgacgaa ttattcctcc ttgacttttt gaaaccatgg
    12421 tcaagcttta aattaattga aaataaaaaa tagataatta gttattgcag aaataatccg
    12481 ctagaaatag ccagttattt ctgctttttt attttgtttt tttataggtt atgatttgtc
    12541 aaattaagct agaataaaat gcttcttg
//
LOCUS       DBKOIJ010000076        12493 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000076 DBKOIJ010000000
VERSION     DBKOIJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.078"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..375
                     /locus_tag="ACV5NJ_001662"
     CDS             <1..375
                     /locus_tag="ACV5NJ_001662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485750.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACV5NJ_001663"
     CDS             complement(324..893)
                     /locus_tag="ACV5NJ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HHY6485751.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACV5NJ_001664"
     CDS             complement(967..2256)
                     /locus_tag="ACV5NJ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485752.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACV5NJ_001665"
     CDS             2514..3458
                     /locus_tag="ACV5NJ_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHY6485753.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACV5NJ_001666"
     CDS             3472..4545
                     /locus_tag="ACV5NJ_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HHY6485754.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACV5NJ_001667"
     CDS             4535..6706
                     /locus_tag="ACV5NJ_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HHY6485755.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACV5NJ_001668"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACV5NJ_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HHY6485756.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACV5NJ_001669"
     CDS             complement(9009..11195)
                     /locus_tag="ACV5NJ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HHY6485757.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACV5NJ_001670"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACV5NJ_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HHY6485758.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACV5NJ_001671"
     CDS             complement(11417..11857)
                     /locus_tag="ACV5NJ_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HHY6485759.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACV5NJ_001672"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACV5NJ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2631 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aac
//
LOCUS       DBKOIJ010000077        12171 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000077 DBKOIJ010000000
VERSION     DBKOIJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.079"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(133..297)
                     /locus_tag="ACV5NJ_001673"
     CDS             complement(133..297)
                     /locus_tag="ACV5NJ_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485760.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="ACV5NJ_001674"
     CDS             592..1920
                     /locus_tag="ACV5NJ_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HHY6485761.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="ACV5NJ_001675"
     CDS             2122..2955
                     /locus_tag="ACV5NJ_001675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HHY6485762.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="ACV5NJ_001676"
     CDS             2971..3708
                     /locus_tag="ACV5NJ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HHY6485763.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="ACV5NJ_001677"
     CDS             complement(3863..4831)
                     /locus_tag="ACV5NJ_001677"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HHY6485764.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="ACV5NJ_001678"
     CDS             5063..5896
                     /locus_tag="ACV5NJ_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HHY6485765.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="ACV5NJ_001679"
     CDS             5930..6769
                     /locus_tag="ACV5NJ_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHY6485766.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="ACV5NJ_001680"
     CDS             6795..7355
                     /locus_tag="ACV5NJ_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6485767.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="ACV5NJ_001681"
     CDS             7554..9275
                     /locus_tag="ACV5NJ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HHY6485768.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLVVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="ACV5NJ_001682"
     CDS             9440..10582
                     /locus_tag="ACV5NJ_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HHY6485769.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="ACV5NJ_001683"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="ACV5NJ_001683"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HHY6485770.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2041 c         2527 g         3455 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagttgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DBKOIJ010000078        12167 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000078 DBKOIJ010000000
VERSION     DBKOIJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.080"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(32..310)
                     /locus_tag="ACV5NJ_001684"
     CDS             complement(32..310)
                     /locus_tag="ACV5NJ_001684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485771.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(425..1042)
                     /locus_tag="ACV5NJ_001685"
     CDS             complement(425..1042)
                     /locus_tag="ACV5NJ_001685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485772.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1437..2261)
                     /locus_tag="ACV5NJ_001686"
     CDS             complement(1437..2261)
                     /locus_tag="ACV5NJ_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HHY6485773.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2604..3020
                     /locus_tag="ACV5NJ_001687"
     CDS             2604..3020
                     /locus_tag="ACV5NJ_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6485774.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="ACV5NJ_001688"
     CDS             3017..3487
                     /locus_tag="ACV5NJ_001688"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6485775.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="ACV5NJ_001689"
     CDS             3501..4274
                     /locus_tag="ACV5NJ_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HHY6485776.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="ACV5NJ_001690"
     CDS             4288..5100
                     /locus_tag="ACV5NJ_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHY6485777.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="ACV5NJ_001691"
     CDS             5154..6119
                     /locus_tag="ACV5NJ_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HHY6485778.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6255..8978
                     /locus_tag="ACV5NJ_001692"
     CDS             6255..8978
                     /locus_tag="ACV5NJ_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HHY6485779.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8991..9623
                     /locus_tag="ACV5NJ_001693"
     CDS             8991..9623
                     /locus_tag="ACV5NJ_001693"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6485780.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9831..10055
                     /locus_tag="ACV5NJ_001694"
     CDS             9831..10055
                     /locus_tag="ACV5NJ_001694"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HHY6485781.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10157..10390
                     /gene="relB"
                     /locus_tag="ACV5NJ_001695"
     CDS             10157..10390
                     /gene="relB"
                     /locus_tag="ACV5NJ_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HHY6485782.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10380..10649
                     /locus_tag="ACV5NJ_001696"
     CDS             10380..10649
                     /locus_tag="ACV5NJ_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HHY6485783.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10828..12129)
                     /locus_tag="ACV5NJ_001697"
     CDS             complement(10828..12129)
                     /locus_tag="ACV5NJ_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HHY6485784.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3973 a         1973 c         2387 g         3834 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcagatgcag cgattaaatg actatcttta tcaactaaca ttgcttttga
     6301 aatctacgcc ttcagataat tattcagctc gcagtatttc gcaagctcta cagttgaata
     6361 gaagtacaat cagtagctac ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa
     6421 aaagttaccc ggttttattt ttacatagaa cagctcttga agaactacat atcacactga
     6481 ataactccga aatagaatca attgaaagct tgttaacaat ttctcaaaaa cccgcattag
     6541 atcaagtaat tggctctaaa ggaagtttga aagaagcaat tgatcaaatt aaaacggcag
     6601 tcttatatcc tggaaaaggg ttgcctttat tattgatcgg cgctagtgga tcagggaaaa
     6661 cctttctagc gaataaaata tatgaatatg cagtagaaga aaaggtcatt cacacatcgg
     6721 cacctttcat agattataat tgtgcacaat atgtgagcaa tccagagtta ctatcctctg
     6781 ctttatttgg ctacacgaaa ggagcattta ccggagcaag ccaggagcat actggtttgc
     6841 tagaaaaagc cgatggagga gtattgtttt tagatgaagt gcatcgctta tctgaagaag
     6901 ggcaagagaa attatttaca tttatggata cgggtgaatt ctctcctatg ggggataata
     6961 gtattcggaa aaaagccgat gttcgattag tctttgcaac aacagaaaat atatatacga
     7021 ctttcttacc aacttttctt aggagattgc cagttattgt taatctacct agatttcaac
     7081 aacgtccgtc gtttgaacgt ctgtcattga ttgatgaatt ttttgtttct gagagccaaa
     7141 ttttagctaa ggaactatct gtttctgatg cattgattca tttcttaatg aatagtgatt
     7201 ttgaaggtaa cgtggggaat attaaaaata ttatcaagta tgcttgcgga aacgcatata
     7261 tccaccagaa gagtcagcaa acaattcaag ttaaattatt ggatatgcct ttagagtaca
     7321 gttataaatt caaggagcaa ctaaataaac ccagaaagaa aagagcagat cgaatctact
     7381 atccagaaga aacacgacaa gtgcaactag aaagaaacaa tcagcttttt gttcaatttt
     7441 ttgaccaatt acttactgga ttctctgaag tgattgaaaa taacaaggat attaaactat
     7501 ttttagaaga aatggttacc aaggtaacac aagtaatgga tacgcttatt tttcaagagg
     7561 attacgaaaa agagcagtct ttatacgcca ttctaaacta tcatattcgt caaagtattg
     7621 attttatgcg agaaagctac gggtttgaac aagatggtaa ccgggtagtc gctttggcca
     7681 actatctata tgtcaaggat aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa
     7741 ccaaagatca gcttaacgaa ttttttgcta atgttcttga aaccccttat tggtatgcaa
     7801 aaaaaatctt aagcctttta tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt
     7861 tctttattac gctatatttt tatagtttga atgtcacgaa tgttaaaaca ggtattaaga
     7921 gtattgtgct agctcatggt tattctacag ctagtagtat ggcgaatgtt gccaaccgta
     7981 tgctgaataa gaacgttttt caagcttatg atatgccttt agatattaca ttagagaaaa
     8041 ttgaagcaaa attgattcaa ttccttggag attatcgaac agattcaggc ttgattttat
     8101 tggtagatat gggttcacta aaccaactag gcggtttatt agtgaatcat ctaaatggac
     8161 cactgttaat cattgatcat atcaatacac cgttattatt ggaagtcggc caacaagtat
     8221 taaacaataa aagtatcacc gaaataggag cagaaattga aacaagtctt actttcaaaa
     8281 agcagttact acttcccaag aaaaagaaaa agaaagccat cattacttgt tgttatacag
     8341 ggattggtag tgcgacccag attcaggaga ttttaacgaa atgcttgaaa gatgcagtta
     8401 gagaattagt aattattccg tatgattaca aaaagctgtt agaaaacaag aattttgaga
     8461 ctccttttca attatacgat gtcatcatga ttgtaggtac agaagatccc aaaattaatc
     8521 agattccata tattggatta gatcgcctga ttaatggggg aagcattggc gaatttgttc
     8581 attacttgga aaaagagctg actattgaag tgaacgattt acaaaaagaa ttaattttca
     8641 atttttcaat tagtaaaatt gtcgaaaatt taaccatttt ggatccagat aaggtgctat
     8701 cttttgtaca aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata
     8761 atcagctgtt tttgttgtac cttcactgta gttgtatgat tgaacgaatt gtacgtaaag
     8821 aaagtgtgga tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg
     8881 ataaaattca tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt
     8941 tagagttgcg attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg
     9001 gagtaatttt tgatatggat ggggtactca tagatagcga gacattttat tttgagagaa
     9061 gaatgaactt ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg
     9121 tggggaaaac agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag
     9181 aggaacttta tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag
     9241 ctttacgaaa agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg
     9301 cactggcatc ttcttctcca gctgtcgaga tagagagtat gctcattcaa tgtgaactta
     9361 ctgaatattt ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg
     9421 aaatatattt aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt
     9481 caacgattgg tattaaatca gcaaaggcag caaatatcta tacggcggca ctaaaacaac
     9541 caatgcaaat cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac
     9601 aaattgtttt agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc
     9661 tattttgact aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca
     9721 ccttttacga aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg
     9781 ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacaga
     9841 accaaaatac gaatagttat caagcagcca tattgactgc ggtcaattta ggaggggata
     9901 ctgatactac tgctgccgta actggtgctc ttgcgggtat tctttacggg ggtgaagtga
     9961 tccctgaaaa ttggagaaaa tcgcttcaaa atgaccagct tcttaaaacg atcagtcaga
    10021 gattttgcga taccgtgcta tctcaaagaa tttgaataat ggatatgatg caaatcttgt
    10081 ttgtactaac gttgtattga caaaaaggat tttatttagt acaatgttag tacaaatgat
    10141 gaacggaggt agaatcatgt ctcaatcaac cattacattt aggattcccg acgatgaaaa
    10201 agaactggta ttagaatatg caaaagcaca caattcttca ttgaccgaac tttatcgaaa
    10261 tgctgtactg gataaaatcg aagatgaaat cgatctacgg actttacgga gtgcaattga
    10321 agtttccaaa gagaaaaaag agagtggtat ttctcaagag gaaatggaga aactgctaaa
    10381 tgaattataa agtcatctac atgccgcaag cccagaagca attaaaaaaa atggacagaa
    10441 accaagctaa aataattgtc gcctggatca gaaagaattt agaaaacacg actgacccgc
    10501 gtcagcacgg aaaaggtttg actggaaata agagtgggga atggcgctat cgaattggaa
    10561 actatcgaat attggctaat atttgtgatt ctgaaattga aatccagata ttctcaattg
    10621 gacatagaag tacaatatat aaaagataac cgtttttgga acgaagtaga gtttcgaaag
    10681 tcaatgtata atcttttgaa ttttgcataa taccacaggt agaaactacc cgatactatc
    10741 ggttggtttc tgctttttta tggctctttg tcaaatagga ctgatagagt gagataagaa
    10801 catctaaggt agaattcctt ttctacttta gatgtttttt gattcatagg taatcagatt
    10861 atttagtaag aaaattctgg tcttcatttt ccgaaagctt cgaaagccat aagcgactcg
    10921 tttcaatgct ttgatgtggg tatgcagatt ttctattttg gcatttgaat aaggcaattc
    10981 gagggctaaa cgaatgccgg attcatagtt tataaaagct ttttttatag aatgcagttc
    11041 ttttggaacg gaattgggca tcgttcttac aagtttaaag aaagtgtccg cctctttgtc
    11101 gtgaaacgct tgaagaattt cttgatagta aaaataggcg attcgtaatt cgtcagagat
    11161 cgaaagcaga cgatccacca cttcactttc ggtgagatac gcccaaccaa aattgcgcca
    11221 tttggtccat ttgtcataaa caagcttgtc ctgtcgtttg atcagaagcc gagacaacgc
    11281 ctttaattgt cgataatatt tggcttgtgt tggatcgttt cgatcaaagc ttttcatgat
    11341 tcgtacgcga atatcctcaa atttacgaat gagatgtttg acaatgtgaa aacgatccac
    11401 aacgagtttt gcctttggaa aacattcctt tatcagagag gcataagaaa cattcatgtc
    11461 cgtcacgatg acctttacct tcaatcgtgc gcgtcgttca aattgcataa aataagaaac
    11521 gagagccttt ttcttccgtg aaggaagaat atccagtaat tttccagtat cgccatccat
    11581 acaagaaaag gagtagggac cgacttgatt tttcagtgaa cgaaattcat ccattaataa
    11641 acaacgcggt aaccaatttc tttttacttt tgtcatgggg atcagagaag cgagtgtccg
    11701 agaaacggtc gaccaagaaa cgccgcaatg cttagcaatt gtcgtcattg cttgtttttc
    11761 agttagctct tctaaaattt tatagcgcac ttgtttagcg ataaagcagt ttctatcgac
    11821 aatgtaggta ttcgccgtaa aataggtgtg gcagccacga cagtggtagc gttgtttcgc
    11881 taaagcaaga taagtcagtt ggttcgcaca tttattaagt tgaatgagcg attttttctt
    11941 tccgttctta acgatcatgc cttcatgatt catctgttta cacaaaggac agcgacgaat
    12001 ttctttactt aacgtcgcag aaacaacaag actcttgata ttcttaatgc tcttcgattc
    12061 cacatttgtg atgaaaatgt ttttatcttt gattccaagt agtagtttga tatgatgaag
    12121 atcaggcata cagaacgctc ctttgattgt gttgtggtga ttcaatt
//
LOCUS       DBKOIJ010000079        12146 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000079 DBKOIJ010000000
VERSION     DBKOIJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.081"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(94..651)
                     /gene="frr"
                     /locus_tag="ACV5NJ_001698"
     CDS             complement(94..651)
                     /gene="frr"
                     /locus_tag="ACV5NJ_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HHY6485785.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(654..1376)
                     /gene="pyrH"
                     /locus_tag="ACV5NJ_001699"
     CDS             complement(654..1376)
                     /gene="pyrH"
                     /locus_tag="ACV5NJ_001699"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HHY6485786.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1512..2393)
                     /gene="tsf"
                     /locus_tag="ACV5NJ_001700"
     CDS             complement(1512..2393)
                     /gene="tsf"
                     /locus_tag="ACV5NJ_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HHY6485787.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2492..3274)
                     /gene="rpsB"
                     /locus_tag="ACV5NJ_001701"
     CDS             complement(2492..3274)
                     /gene="rpsB"
                     /locus_tag="ACV5NJ_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HHY6485788.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3633..4112
                     /locus_tag="ACV5NJ_001702"
     CDS             3633..4112
                     /locus_tag="ACV5NJ_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HHY6485789.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4329..5420
                     /gene="phnW"
                     /locus_tag="ACV5NJ_001703"
     CDS             4329..5420
                     /gene="phnW"
                     /locus_tag="ACV5NJ_001703"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HHY6485790.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5413..5541
                     /locus_tag="ACV5NJ_001704"
     CDS             5413..5541
                     /locus_tag="ACV5NJ_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HHY6485791.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5545..6090
                     /locus_tag="ACV5NJ_001705"
     CDS             5545..6090
                     /locus_tag="ACV5NJ_001705"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HHY6485792.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6544..8235)
                     /gene="argS"
                     /locus_tag="ACV5NJ_001706"
     CDS             complement(6544..8235)
                     /gene="argS"
                     /locus_tag="ACV5NJ_001706"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HHY6485793.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     misc_binding    complement(8312..8509)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8655..9602)
                     /gene="arcC"
                     /locus_tag="ACV5NJ_001707"
     CDS             complement(8655..9602)
                     /gene="arcC"
                     /locus_tag="ACV5NJ_001707"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HHY6485794.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9717..10736)
                     /gene="argF"
                     /locus_tag="ACV5NJ_001708"
     CDS             complement(9717..10736)
                     /gene="argF"
                     /locus_tag="ACV5NJ_001708"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HHY6485795.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10827..12056)
                     /gene="arcA"
                     /locus_tag="ACV5NJ_001709"
     CDS             complement(10827..12056)
                     /gene="arcA"
                     /locus_tag="ACV5NJ_001709"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HHY6485796.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3324 a         2521 c         2199 g         4102 t
ORIGIN      
        1 aataattttt tcttttttta atttttaaat aaaagaaata taaataaaaa cagaggaagc
       61 ggaatgatcc gcccctctgt ttttatgatt ttattaaact tccaataatt ctttttcttt
      121 atcagcagcg atcttatcta cttctttgat gctgttatct gtcaatgttt gtacgtcttt
      181 ttctaatccg cgcaaatcat cttcggtaat atcaccattt ttttgttgtt ttttatattc
      241 atcgatcgca tcacgacgga tgttacgaat cgcgattttt gcattttctg cttctttttt
      301 cacatcttta gctaattctt ttctgcgttc ttctgtcagt tgcgggataa ctaagcgaat
      361 gacatttcca tcattggttg gactgattcc gatatcactt gccataatag ctttttcaac
      421 atcttgaaga atgctcttgt caaaaggtgt gatcattaaa acacgtgctt ctggaatttg
      481 gattgatgcc atttggttca atggtgttgg cgcaccgtag tagttcacag taatacggtc
      541 caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt gaagattttg
      601 tgctgctttt ttcatttttt cttttgcttc tgtcataatt gcatctgcca tttttatttc
      661 ccccttacag ttgttccgat attttcgcct aagattgcac ggcgaatatt tccgtgttca
      721 tttaaattga agacaagcag tggaatatca ttatccatgc ttagtgaact tgcagttgaa
      781 tccatgactt gtaatccttt ggcaataact tccagatgag tcagttcttc aaattttact
      841 gcatttgcat ctactttcgg atcagcagaa taaacaccgt ctacattgtt tttagccatc
      901 aggataacat ctgcgccgat ttctgccgcg cgtaatgctg ctgttgtgtc tgtcgagaag
      961 tacgggttgc cggtaccgcc tgcaaagata acgatccgtc ctttttctaa atgacgttct
     1021 gctttacggc gaatatatgg ctctgcgatt tgacgcattt caatcgaagt ttgcacgcgt
     1081 gtcggtacac ccacgttttc caacgtatcc tgtaaagcta aggcattcat aactgtagct
     1141 aacataccca tgtaatcagc ttgagcacgt tccatgccca tttgtgctcc gatttgtccg
     1201 cgccagatat ttccgccgcc cacaacgatt gccatttcaa cgcccagctc gtgaacttct
     1261 ttgatttctt ggatgatttc tttgattact ggtggtttga ttccaaatcc tgcgtcccca
     1321 gctaaagctt ctccactcaa ttttaagact acacgctgat atttcggtgt gaccatgttc
     1381 attcctccaa tattttctat tgccatttta ccatacttag atgatttcga cactctaaga
     1441 ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt cccgtctgtt
     1501 attcaacaga attatttttt cacttggctc ataacttctt ctacaaagtt atcttcgcgt
     1561 ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt taacagttgc gccttttgat
     1621 gcaacgtatt tttcaacagt catatcagga tctttaacga atggttggtc caccaaagcg
     1681 atttctgctt tgaatttctt caagcgtcct tctaccattt tttcaacgat attcgctggt
     1741 ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt catgttctaa ttcagcttct
     1801 ggaatttgtg attcgtttac ataacgagga ttgatagctg ctacgtgcat agctacgtct
     1861 cttgcaactg tttcgtcagt tgttccatct agaacagtca atacggcaat acgtccgccc
     1921 atatgcaagt atccaccaaa tgcagcattg tcgtcttttt ctacaacttc aaagcgacgg
     1981 aagctgattt tttctccgat aacttgagtt gcttcgatca agtcagattc aattgtacct
     2041 ttatctgttt tgattttcat tgcagcttcc atgtctgctg gtttgttttc agcaacaagt
     2101 tctgcaattt cttttactaa atcttggaac atttcgtttt tagaaacaaa gtctgtttct
     2161 gagtttactt caacgattgc tgcagtgtta cctttcacag caactgaagc caatccttca
     2221 gctgcaatgc ggtcattttt cttcgcagcc tttgccattc ctttttcacg taaaagatca
     2281 actgcttttt ccatgtcgcc ttctacttca actaatgctt ttttcgcatc catcattcct
     2341 acgccagtca tttcgcgtag ttcttttacc attttagctg taacgtctgc catgtttaac
     2401 tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga ggcttgtcgc
     2461 ccagcctttg aaacagctcc atcattcaaa attattctgc tgaagaattg tcgccttcaa
     2521 caacatctac gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa tcttcttcaa
     2581 ctacttgatc ttcaccttgg tttccttcga tgaaagcatc agccattttt gaagtaatca
     2641 atttaacggc acgaattgca tcgtcgtttg aagggattac tacgtcgatt tcatctggat
     2701 cgcagtttgt atcaaccata gctacgattg ggatatttaa tttgtgtgct tcttgaacag
     2761 caatacgttc tttacgtggg tcaacgatgt acattacatc tgggattctt ggcatatcag
     2821 cgataccgcc taagaatttt tcaagacgtt cacgttcttt gttcaaacca gcaacttctt
     2881 ttttaggtag aacttcaaaa gttccatctt cttccattgc attgatttgt ttcaaacggc
     2941 tgatacgttt ttggatagta tcccagtttg tcaatgttcc gcctaaccaa cgatggttaa
     3001 cgaagtattg tcctgcacga gttgcttctt ctttgattgc ttcttgtgct tgtttttttg
     3061 tacctacgaa taatgctacg ccgccttctt cagcaacgct cttcatgtaa tcataagctg
     3121 catctactaa tttaactgtt ttttgcaagt caatgatgta gattccgttt ctttctgtga
     3181 agatatattt cttcattttt ggattccagc gacgagtttg gtgaccaaag tgtacgccgg
     3241 cttctagtaa ttgtttcata gaaattactg ccatgtttgt ttcctccgat ttggttttat
     3301 ttttcctctt cttatcttct accttgccgg caaacttttt caagcaccta ccgacaatcc
     3361 aataagaatg tggatttgct gatgcataaa gcaccctcag ctattataca gacaaaagat
     3421 ccgtatcgca agagcaaata tacttttctg ctcaacattc aatagtttac ctgaaagata
     3481 aaaagaattc aagaaaaaca gtagatttct ctttgtttct ttattatcct ttttccataa
     3541 aataatttta ggtataaaag acgaatatta ttgggcaatc cttctttttt ttgttatgat
     3601 acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga catttgctga
     3661 ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga agaactatta aacaaacttg
     3721 ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg agatatccgt gatctaaaaa
     3781 tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt attgtttcgc gaaaaaggaa
     3841 ctcctgagac taggactgag gaagaggatc ggctggttca aatgattgaa gacattgtat
     3901 tgaaagtcga acgagtccac tttttgacga tcgtcaatac cttgccagac aatgctcatt
     3961 tatttgcatc tgttttagat gagatcaaac ctccctctat tgtcagtaca attgctggat
     4021 tcgatacgat catggtcatc tcggaaaatg aggaggacgc aaaacgagta gaagaatttc
     4081 ttcaccatcc aactgaagta tcactttttt aatcagagca cgtattccta atatggaatg
     4141 cgtgtttttt tattttcaat attttcctta ttgaataaac ctttacactc tttacaaaaa
     4201 tacatcattt tttattcaaa tttttcaaca ccttgattga atcacaataa tcatcattta
     4261 tattggatgt taacaagagc agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag
     4321 gaaatcatat gtataaatta ttaacttctg gaccattgac gacttctgaa acagtgaaaa
     4381 atgctatgct gatcgatcat tgtacttggg atcaagaata caaagaaatt acacaatgga
     4441 taagaaaaaa attattggat cttgctcaag tatcggaagg aatatacaca acggtcttaa
     4501 tgcaaggtag cagctccttc ggggtagaat cggtattaag ttctgtgatc aggccagaag
     4561 atactgtact gatttgttca aacggcgcat atagacaacg aatgatcaac atgtgcgaat
     4621 gtctgaaact gaactatacc gtttatgagg tatcggaaca tcaaatcctc caagctcatg
     4681 tgattacaca gctgattgaa gagaatccgt ctatctccgc actagcaatg gtccatagcg
     4741 agactacttc cggtattttg aataatttgg aagagattgc tgctgtcaca caagaaagag
     4801 atcttttgtt tatagtggac gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg
     4861 aactagggat tgattttttg atttccagct caaacaaatg tattcaaggt gttccaggct
     4921 tttcttttgt acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt
     4981 tgtcacttga tctatatgac cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt
     5041 tcacttcccc tacccatacg gttttggctt ttcaacaagc gctgaaggaa ctagaggcag
     5101 aaggaggaat cgaaactcga tattcacgtt atctcgccaa taatcatgtg ctacgacaaa
     5161 aaatgacaga gatggggttc cagcctttca ttcaagaata tcaggggcca ttcatcacct
     5221 cttttcttta ccctaaggac tggcgatttg attttaatca tttttatcag tatttaaagc
     5281 aaaatggatt tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa
     5341 atattggcga gatctatccg gaagatattt tggaactgac agcaattatc aaagactata
     5401 aggagaacgt taatgattaa aactgtaatt tttgattggg caggaacaac tgtggacttt
     5461 ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg aacccaactg
     5521 actgacaaag aaatccgcta accgatggga aaattaaaat gggatcatat ccaacagctt
     5581 ctttccctcc cttctgtcaa aaaacaatgg atagctattt atggacaact acctcaggaa
     5641 aaagaggtgg aggaattgta tcaacaattt gaacattatc tgtttaaaga tttggtcaag
     5701 cattctacct tgaaacccga cacattggaa acgatcgaac ggttgagaaa cgctgggatc
     5761 aatattggtt ccacgaatgg atttaatgct cctatgatga caatcgtagc agaaaccgca
     5821 aaaaaagaaa ggtattcacc agacttcatg gcaacttttg aagacgtcga agggtatggt
     5881 cgaccgtttc cttatatgat ccataaaaac atgcagtact tcaaaaacaa gtcggttgat
     5941 gaagtaatca aagtcggaga tacggtctca gatatccagg aagggaaaaa tgccggagtg
     6001 ttgacagtag gtgtaattga aggtagttcg ctaatgggga tttcttatga tgagtaccaa
     6061 aaattagaga tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga ttccaacaag
     6121 ccggcgctga tatttgtgta aacaatttaa aagagttaac agaatacatt ttgtacgctg
     6181 acaagaatcc agaatgactt ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc
     6241 aagtaataaa aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttaggcttat
     6301 taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac
     6361 gacttttgtc acagtcccct tatttgcaaa caaacagtgt ttaagagaca acctctccac
     6421 taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa
     6481 gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt
     6541 ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac
     6601 agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt
     6661 cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac
     6721 tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt
     6781 aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa
     6841 tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt
     6901 ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt
     6961 tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt
     7021 ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa
     7081 tacttcttct aataagacga tctttccttt acgagttgat aattttttac caccttgcgt
     7141 gatcaaacca aatggaatat ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa
     7201 gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg
     7261 tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag
     7321 tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac
     7381 gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc
     7441 atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag
     7501 atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat
     7561 agcctcttta tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc
     7621 tggttctgtt tcagctactt catggaattg gacgtataaa cgaagcaatt cattgattgg
     7681 ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa
     7741 ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat
     7801 aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt
     7861 ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga tgttgctatc
     7921 cccataatgt tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt
     7981 attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc
     8041 gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa
     8101 tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg
     8161 ctcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag
     8221 atctttatta ttcatcttta ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa
     8281 tctctagcga cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac
     8341 tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa
     8401 aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga
     8461 attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa
     8521 ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa aaaaaaaatc
     8581 cagatggcct aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg
     8641 aaagacttaa caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc
     8701 tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca
     8761 atagctgctt ctactttcgg aagcatgctt cctggagcga attgcttttc atcaatgtat
     8821 tgtttcattt ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg
     8881 acatacacat tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct
     8941 aatttttctg aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct
     9001 gtttctacaa caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc
     9061 agcgtttcga tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt
     9121 ctccatccac gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct
     9181 ttggcttctt cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga
     9241 tcattttcat ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt
     9301 tccttcagta cttcgcccat ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt
     9361 gcaacacatg tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga
     9421 atcaataggt taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt
     9481 tcgataaatt tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca
     9541 cttgcatccg tagacaagat ggcattgccc cctaaagcga caactacttt tcgtttggcc
     9601 atttcattcg tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt
     9661 tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa
     9721 acgcgaggga taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata
     9781 cgattttctg cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact
     9841 tccatttcag tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca
     9901 tggaaagctg gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc
     9961 atttccatat tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct
    10021 tcacccattg atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca
    10081 acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg
    10141 acgatttctt cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa
    10201 attgcacctg tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca
    10261 acagtgatac cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt
    10321 tgtgttggat gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct
    10381 agttcctcta ccattttttg actgaatcca cggaattcga ttccgtcaaa catacgtcct
    10441 agtactttag ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct
    10501 agatattcag gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt
    10561 gtagatgttt tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata
    10621 ccacgttttt tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct
    10681 tttgtaaagt ctttttctgc taataagctt cttccttgga atactgattc tttcatatta
    10741 aacattctcc tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga
    10801 cagtaagact gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc
    10861 atgcagcgtg gaccaccacg acctcgtgac aattcacttg aattgatttc taacactttg
    10921 atgccatagc tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca
    10981 cctggagcga ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc
    11041 tcatctccat taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc
    11101 gtttcgtgta agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta
    11161 aagacatcca tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga
    11221 tctaccattg taaatacggt atccaaatgc atcattgcac ggttgttcgg aattttgatt
    11281 gccaacactt tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa
    11341 gctttcgcat tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat
    11401 tcgtctccgc cttcgatatg atctggattt tcacgatcca accatacttc gattccttta
    11461 tttgcaaatc ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt
    11521 gcttcaaaag tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt
    11581 gtaaagtaca agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga
    11641 tcatatagac tgctagaacg tacttcgata tcttttgtac gaaccccagc catgatttta
    11701 tctaccattt cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca
    11761 ttcgatgcaa tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct
    11821 gcgtcaatcg cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc
    11881 aatgtcttcg caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg
    11941 aacaacagac gatccataat atctggtgtc aaattttcca cttcttgtcc aggccgtttc
    12001 agtaaaactg tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg
    12061 tgttgcctcc ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat
    12121 aattttgtct cattcaaaat gagaca
//
LOCUS       DBKOIJ010000080        12114 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000080 DBKOIJ010000000
VERSION     DBKOIJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.082"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            140..460
                     /locus_tag="ACV5NJ_001710"
     CDS             140..460
                     /locus_tag="ACV5NJ_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485797.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACV5NJ_001711"
     CDS             912..1112
                     /locus_tag="ACV5NJ_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HHY6485798.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACV5NJ_001712"
     CDS             1259..4048
                     /locus_tag="ACV5NJ_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HHY6485799.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACV5NJ_001713"
     CDS             4095..4622
                     /locus_tag="ACV5NJ_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HHY6485800.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACV5NJ_001714"
     CDS             4643..5833
                     /locus_tag="ACV5NJ_001714"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HHY6485801.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACV5NJ_001715"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACV5NJ_001715"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HHY6485802.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACV5NJ_001716"
     CDS             7966..8640
                     /locus_tag="ACV5NJ_001716"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HHY6485803.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACV5NJ_001717"
     CDS             8637..8978
                     /locus_tag="ACV5NJ_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485804.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACV5NJ_001718"
     CDS             9008..9367
                     /locus_tag="ACV5NJ_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HHY6485805.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACV5NJ_001719"
     CDS             9627..9749
                     /locus_tag="ACV5NJ_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485806.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACV5NJ_001720"
     CDS             9891..11975
                     /locus_tag="ACV5NJ_001720"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HHY6485807.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4047 a         2113 c         2603 g         3351 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataa
//
LOCUS       DBKOIJ010000081        12106 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000081 DBKOIJ010000000
VERSION     DBKOIJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.083"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(228..2303)
                     /gene="glyS"
                     /locus_tag="ACV5NJ_001721"
     CDS             complement(228..2303)
                     /gene="glyS"
                     /locus_tag="ACV5NJ_001721"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HHY6485808.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2305..3222)
                     /gene="glyQ"
                     /locus_tag="ACV5NJ_001722"
     CDS             complement(2305..3222)
                     /gene="glyQ"
                     /locus_tag="ACV5NJ_001722"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HHY6485809.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3608..4426)
                     /gene="recO"
                     /locus_tag="ACV5NJ_001723"
     CDS             complement(3608..4426)
                     /gene="recO"
                     /locus_tag="ACV5NJ_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HHY6485810.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4569..5468)
                     /gene="era"
                     /locus_tag="ACV5NJ_001724"
     CDS             complement(4569..5468)
                     /gene="era"
                     /locus_tag="ACV5NJ_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HHY6485811.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5483..5878)
                     /locus_tag="ACV5NJ_001725"
     CDS             complement(5483..5878)
                     /locus_tag="ACV5NJ_001725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HHY6485812.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5862..6338)
                     /gene="ybeY"
                     /locus_tag="ACV5NJ_001726"
     CDS             complement(5862..6338)
                     /gene="ybeY"
                     /locus_tag="ACV5NJ_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HHY6485813.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6367..8559)
                     /locus_tag="ACV5NJ_001727"
     CDS             complement(6367..8559)
                     /locus_tag="ACV5NJ_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HHY6485814.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8583..9554)
                     /locus_tag="ACV5NJ_001728"
     CDS             complement(8583..9554)
                     /locus_tag="ACV5NJ_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HHY6485815.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10259..11638
                     /gene="brnQ"
                     /locus_tag="ACV5NJ_001729"
     CDS             10259..11638
                     /gene="brnQ"
                     /locus_tag="ACV5NJ_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HHY6485816.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11920..>12106
                     /locus_tag="ACV5NJ_001730"
     CDS             11920..>12106
                     /locus_tag="ACV5NJ_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HHY6485817.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         3317 a         2568 c         2204 g         4017 t
ORIGIN      
        1 tttttttgac tagggaaatt cctaatttaa aagtaaataa aacagtaaca agattataat
       61 taatcgtttt catttttatt tcctcacttt tcatgagcgt tttgaaaatt cgaacaaaga
      121 ataaaataac aaaaattatt aaaatagaat taataacagc tatttataac aaaaaaaaca
      181 gagacacagc acgtttcgtg catgctctct gtttttcaaa caaaagacta ttttgtgatg
      241 atttgatcca agcttgctaa tgacaaagcc atggcagcta tttgacttag ttgtgttagt
      301 cggttcttac gtactgctgg ttcatcagcc atgaccatcg tttgatcaaa ataattttcg
      361 atcagtggac gaagagcgac aagctggtcg tagttttcgt ggatcgtttg gtcagcaaaa
      421 cttgctttca ttgcttctac tgctttgtgt aattctttct ctgcgtcgtt ttcgaacagc
      481 tcagggtcta cttctgattg tttttcaagc tgcgcttttt tcgctaagtt cacgacacgt
      541 gtcaaagctt ccattgaagg tttgaagtct tcatctgcaa gatgttgttt gaacaattgt
      601 gctgctgcaa atacttttgt cagatctttt tgttccgcat tcaaaacagc atcgatgaca
      661 tcatgacgaa tattcttcgt tgtcaataat tggcgcagac ggcctttgat aaagtcgatc
      721 acttgttgtt gcccttctga taaaccaatg ccgaaacgat ccgtatcttt actgataaca
      781 tcgctaatcg tttcttgcag aacagacaaa ggaaaggccc attttttcga ttcgatgatc
      841 cgaacgacac cgtatgcttg gcgacgtaag gcataaggat cgtttgaccc gcttggaatc
      901 atcccaactg caaagaaaga aaagatgcta tctaatttat ctgcaatcgc taaaacagaa
      961 cctactgtag actgaggtag ctctccttca ctggatatag gcatgtaatg ctcacgaatc
     1021 gctgtcgcta cagcaggttt ctcgcctttc agaagagcat atttttcgcc catgatccct
     1081 tgtagttcag gaaattcccc taccatgttg gttactagat caaatttata gatttcagca
     1141 gctcgttgca aatccatcaa ttcttcctca gatagtccta cttgttcgcc aatgatacgg
     1201 gcaatgactt gtacccgttg cattttctca tagatactac cgattttttc atggaaagta
     1261 acagttttta gtttttcaac gtattggtcg attgaagctt tcttgtcttc attataaaag
     1321 aattccgcat cttctaaacg agccgtcaat actttttgat tgcctcgaac cacatcttcc
     1381 aattttaccc gatcaccatt acgagcagaa ataaaatgag gaagcagcat accttgatca
     1441 ttccgtacct cgaaataacg ttgatgttct ttcatagaag tgactagtac ttcttcagga
     1501 acagaaagat atttttcttc gaagtcgcca gaaaagacag ttggatattc gactaaattg
     1561 ttgacttctt ctagtaaatc ttcgtctaga tcaataaccc agtgattttt ttctgctaat
     1621 tcgtttgctt gtgtaacgat catttcttgt cttttttgcg gagacgcaat gacgaattgt
     1681 tcattcaatt tttcttcata ttcttttgca tgcgcaaatg tcacatcttc gcctaagaaa
     1741 cgatgacctc gagaggctct gcctgttttg acatccaaga tttcgaatgg gattacttcg
     1801 tcatctaaca atgccacgat ccagtgaata gggcggatat attcaaaatc gtattctgcc
     1861 caatgcatcg ttacagggaa agtcaaactt ttgatcaccc ctgctaagcc tgtcaatact
     1921 tcttttgctt ccttaccatg agtgtatttc gttacataga catattccac accttttaat
     1981 tctctaaaag tgatagcgtc tgtcgtcaga ccttgtccgc ggacaaatcc ttccgctgct
     2041 ttcgtccagt ttccatctgc gtccaaagca atttttttgg ctggtccttt gacttcttct
     2101 tcactgtccg cttgtcgttc tggaatattt gttactttga ttgctaaacg gcgcggtgtg
     2161 gaaaatgttt cgatcgtatc ataagtcaat tggttttcat ctaaaaattt tgctgttttt
     2221 tcttctaatt gtttcatgct tggtgtgaca acatgggcag gcatttcttc cagcccgatt
     2281 tcaagtaata agtcttttgc catcttattc agcctccttc acggtttctg gatttgctgt
     2341 gtttttattc aacaaaggga atcctaattt ttcgcgttct gccacaaaga ttttggctac
     2401 agcacgagcc atgttgcgga tgcgagaaag ataaccggca cgctctgtta cggatactgc
     2461 accgcgggcg tctaataagt tgaatgtatg gctacatttc aagatataat catacgccgg
     2521 atgaacaaga ttttcatcga tacagcgttt agcttctttt tcaaaacgat caaagttgct
     2581 tagtagcaag tcttggtcgc ttacttcaaa agaatatttg gaatgttcat attctggttg
     2641 tttgaagatc tcgccatatt tcacgccatc agcccattct aaatcataga cactttcaac
     2701 ttcttggata taagaagcaa gacgttctaa tccataagtg atctcagaag ttactggttt
     2761 acaaggcagt ccaccaactt gctggaagta cgtgaattgt gtgatttcca ttccgtcaag
     2821 ccatacttcc caacccacac cggcacagcc catggatggg ttctcccagt tgtcttcaac
     2881 aaaacggatg tcgtgttcta atggatcgat tcctaaaagg cgcaagcttt ctaaataaag
     2941 ctcttgaata ttctcaggtg aaggtttcat caccacttgg aattgatgat gctgatacaa
     3001 acggtttggg ttttcaccat aacgtccatc agctggtcgg cgagatggct ctacgtaagc
     3061 agcattccat ggttctggtc cgattgcccg taaaaaggta tatgggctca tcgtacctgc
     3121 tcctttttct gtgtcataag cttgcatcag catgcagccg tttgatgacc aaaatttttg
     3181 taatgttaaa atcatatctt ggacggtcaa ttttttactc atctgttttg cccctttcaa
     3241 ttttaaagcg ttgctgtcaa ataagaatac aggaaagaaa acgacaaaaa atccctatgt
     3301 ctgattacag acatagggac gaataattcg cggttccacc ctacttccga ataaagattc
     3361 ggcagcttca tttatcgtac tcgcaagtgc ctcttcaaaa atcgtttggg tccggctccc
     3421 actctcccga actcgctaaa ccacgtgttt tttactgatc ttggtcaatg tacgtattca
     3481 ttttgcagtt ttcttgtttc tataatagat cctatcactg aacgatctat ttaatctatg
     3541 gttaataata gacggaactc cctgtgtttg tcaaatggtt ttgatttttt gttgtatgtt
     3601 ttatttacta ttcttctttt tcaggtgtct ctttttcttt ttcagacatg tctttctctg
     3661 acgtctttaa tacgtgtgcc caagacttca tttgattgat gaactttttg cttttcaaat
     3721 ggataccgac atattcatca tacagctggt cgatgagttc acggattgct tgtttggttt
     3781 cttctttcag atggattcca ctaattttat cataagaaat agctgaaaac atccgaacga
     3841 aataaacagc tcgaggatct gcatgataac ggtgatcgtc catcgaccaa tgtctttcgc
     3901 atatcacacc accatatttg gaagaatagt caaattttcc ttttgtttcc ccgcatacga
     3961 cgcagtgtgt ccagattggt gcaattccga agcgttgcag tatttggact tcaaaaatat
     4021 tcgtgatgat ttctgcatcg tctccttggt ccatccgttg cagtgcttgt tgcgtaaact
     4081 ggaacaaatg cgggtcgtac tgatgatctt cgattgccgc atctactaga ttcaataaat
     4141 atgtgccata gccgctcaaa aagatgtctt cttggatgtg tcgaaacgca tggacatctt
     4201 tgacactgct taaaaatgac aaaccttcac ttcgaaaatt cccgatataa actgcttcag
     4261 taaaagggag caaagccgga gctaagggct gattttgccg atggatctct ttgatataaa
     4321 acatcatttt gccagaagat tctgtaaaga ttttgactaa tttatctttt tctttgtaat
     4381 tacggctaga taaaatcaag ccttttgatt ctgcaagctg gctcatattc tccctcctaa
     4441 aaaataaatc tggaaaagca gattctgccg atccagatca tttatgttgc gatcttccgc
     4501 agcttttctc aacacgcgga tttttattca gtttatttct atacctaatt tacaagaagc
     4561 tcagaatatc agtattcgtc ttttcggtag ccgaagtctt gcagatagac ttttttgtct
     4621 cgccagtctt tttgaacttt gacccaaagt tctagaaaga ctttgtctcc taaaagattt
     4681 tcgatatctt ttcttgcttt cgttcctact tgcttcagca tttttccgcc tttgccgata
     4741 atgatccctt tttggctatc tctttcgaca ataatcgtag cctggatatg gattttatca
     4801 ttctcgtttc gcttcatcga atcaacaacg actgcgacag aatgaggcac ttcgtctcgt
     4861 gttaaaaaca agactttctc acggatcaat tcagaaacga tgaagtattc aggatgatca
     4921 gtcacttgat catctggaaa atattgtggg ccctctggca tttcactgac taatacatcc
     4981 atcaaacgtt cgacgttgtt tccttctgta gcagagattg gaatcacttg tgcaaaaggc
     5041 atctgcgttg aataatcttc gatgattccc aacaactcgt ctggatggat tttgtcgatt
     5101 ttattgataa tcagataaac aggagaatgc acgtttttta gacgttcaat aataaaatcg
     5161 tctccccgac cgcgtttttg atcagcacta atcataaata agaccgcgtc cacttctttc
     5221 aacgcactgt aagctgcttc taccataaaa tctcctaaac gatgctttgg tttatggatt
     5281 cctggtgtat caataaaaat caactgagct tctggtgtag tgtatactcc ttggatttta
     5341 tttctagttg tctgtgcctt gtcactcatg atggcaattt tttgacccac gattcgattc
     5401 aataatgtgg atttccctac atttgggcgg ccgacgattg cgacaaaccc tgatttatgt
     5461 tctggcatat atgttctcct ttttaattaa acaacaaatg atatatttta gggacaaata
     5521 aaatcaaccc aacgatgacg gtaaaacaag cggtcactaa aactgctcca gctgccatat
     5581 ctttgatttt tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc acgacatttt
     5641 caaaaacagt attgatgatc tctactatcc atacgagaaa aacagctaaa agcagccata
     5701 accattctat ccgatcaagt tggaaaatca cgcccataaa caaagcgagg attccgaacg
     5761 caacatgttt cctcatgttg cgttcttcct caaaaacagt tttgactcct tggacggcaa
     5821 attccacgga catcaggaaa tgtttgtttt tctctacgtt tttatctttt aagtccatag
     5881 gcatctaaga tctctttctg taaaccaaac atttctttct catcctctgg tgtcatatgg
     5941 tcatacccgt tgatatgcaa gaatccatga accgctaaaa agccaagttc ccgatcatac
     6001 gaatggccgt aagcttctgc ttgctctgca gcacgttcag tagagatcat gatgtctccc
     6061 agtacacgag gcaatggatt ctctccatct tcaaaaatca ctgggatttc gtcttcgccc
     6121 tcttcttcca aggcaaaact gatgacgtct gtcggtgcat cttttccacg gtaatcacga
     6181 ttgattactt ggattgctgc attgtccatg aaagtaacag acatttctgt ttcttctggc
     6241 aactctaaat atgatgctgc aaattgcagc aagtcgtcga tttctttcat tttctcttct
     6301 gagacttggt ttgtttcatc catgaaggta atatccatta tacatcgctt ctttctattc
     6361 ttcttgctat tcttcttgtt cttcttttat cttctcaact tccgatttca ttttaggata
     6421 tttgatccga gaatggaagg tactgcttaa accgctgatc aatgattttt ctactttacg
     6481 gatttctttt aacgtcaatc cactgtcgtc caattgtcca tcggaaatac gctcttcaat
     6541 aagattatgg acaaaacttt cgattttttc gctcgttgga tggtccattg cccgtactgc
     6601 cgcttcacaa ctgtcagcta tattgacgat tcctgcttca cggctttgag gttttggtcc
     6661 aggataacgg aattgctctt ccgtaacttc cgggttgcgt tcctttgcct taacgtagaa
     6721 ataacgcatc agcgtggttc catgatgctg tctacaaata tcgatcacca tttgcggcat
     6781 attataatct tccaatattt tggcaccatc tgtcacatgt ccaaagataa tatgtttact
     6841 gtcttctggc aataagaaat tgtgcggatt ttccgctcct gaaggcaagt tctcaacgaa
     6901 aaagctggcg tgtttgatct tgccgatatc atgataatag caagctactc gagttagtaa
     6961 agaacgtcca ccgatttctg ctacagcatt agcacttaga ttggctacca tcatgctatg
     7021 gtgatacgta cctggtgctt cttccagcaa ctgtttcaat aaaggatgat tgggattgct
     7081 gagttcattt aatacgatca cactatcatc attgaccatc aattcaatat aggggtgcag
     7141 acctatcgtc agcaagaatg aaaacagtgc acctgtgaat ccgcagacta gcgttaacca
     7201 agtcgttgca tccccaaagc tcattccttg gtagataatc aaaatgacat ccatcatcac
     7261 aggaaaaata atgacccaca tagcagcgga taacccttgt tctgagatcc gtttgcgtct
     7321 gacgatcgtt gccatgagac cagaaaatac ataggacatc aaaataactg tcagaaagtt
     7381 cgtgccaatc gaatcataaa aaatgaaaag tgcggatact gcttgaaaaa taccagccat
     7441 gatccctgct cttcgattca agaaaaagct taaaacaagt ggagcaaaag ccgcggggta
     7501 aaacagcgga atataagcag cttgttctgt ttggaagagt tggaaaaatt tcattagtac
     7561 gacgcttact gacattgccg tcacgtagaa aagtacgtat tttgttcttt ctcctgcatt
     7621 ttggaattga agtgaattat aaatcagtac accgatttga agcaaaacag ctaaaaccat
     7681 ggcaatgagt gggaaaatag aagtcgtttg attcgtcaat cctagtaatt ccaatttttt
     7741 catcgctgaa gcatcaattt gatttccttc gcgaacaata atttctcctt ggaagatcat
     7801 cactggttgt acagcttctc gtgcagattg ttttaattct tctgtctttt tctcattcaa
     7861 tacatcatta actacgatgc cttgatccac taagtagcga atcatttgtt gctgctctgt
     7921 tgtgacattc aaatattgaa tctgatcttc tgcttcttgt ttaaaagctt ccaattcgct
     7981 ttcacgaact tgtttactca tctgttcgtc aatgagcttc aaactttcat ccctaactgt
     8041 gtgaatttcc gtttcagtca tttcaaatat cgtcgaataa aaattgttcg gcagtttttg
     8101 gtaaaacgca acgttttctg cattcacgtt ttcaaaattt ttcttcagtg tagcaatccg
     8161 ctcttctgta gtaggtttcg ggatattttc atcttccttc gccttatcta ccttgccatt
     8221 ataagatttg ttgatggtat cattcgtctt atcgatcaat tcgaataatt gcttgatccg
     8281 attgttctgg tcatctgcca gatctttttg ataagtatat tctggagtca ctgcttctgc
     8341 tgccagtttt cttctttgtt cggtttcttc cgtgttctca atcgttttat tcgcacggat
     8401 actctcttct gctacttggc cttctctgaa atcaatggcc ttttgtttga cactactaaa
     8461 tgtaacgccg cagacaatga taaaaaacaa gaacagaagg aaaggcaatg ctgcctttcc
     8521 gagtttttct tgtagtttct gcagtgtttt attcaacatg tgcttatcct cctaagcaaa
     8581 gcttattctt gatgaagatc cgccttttca taagctttga tgatttcggc aactacaggg
     8641 tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga tttttttgat ttgctttaat
     8701 gttcgttctg catgaacaag accacttgtc gtcccacgag gcaagtctat ctgacttgta
     8761 tccccattga caatcatttt tgattgataa ccgagacggg tcaagaacat tttcatctga
     8821 gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg catcatccaa tgtccgcccc
     8881 cgcatgtaag ctaaaggtgc gatttcgatc acgcctcgtt ccatcaatcg gttcgtatga
     8941 tccaatccaa aaatttggta taaagcatca taaacaggcc gcagataagg atccactttt
     9001 tctttcagat ccccgggtag aaagcctagg ttttcccctg cctccacagc gggccgcgtc
     9061 aaaatgattt tttgcacttc gccttttttt agggcagcaa ttgctaatac tacggcaaga
     9121 aacgttttcc ctgttccggc tggccctaca ccaaatacga tgtcatgttt ggctaccgat
     9181 tcaacgtatt tcttttgacc gctgtttttt acccgtatag gttttccatt acggtctttg
     9241 acgatctctt cctcatacat ctcaacaaaa taatctaatg tacctttttg ccccattttc
     9301 aatgccgtca caacatctgg cgtacttacg tggattcctc gtttgatcaa ttcctgtaat
     9361 gtccgaatga ctgaggcagc caaggaaatc tgggattgct caccaatgat ctgaatgatt
     9421 tctccacgtg tatggatggt tgtttccgtt gtttcctcga tcagttttat atgcttgtcg
     9481 tgtgcaccta aaagcatgct gacatcatca gcagctgtca atctaatctc taaagaacta
     9541 gactcttccg tcaaaaatag acatcccctt tttttcgatt cattctttcc tattttacca
     9601 tactcagaag aaactatata gcaaataaga aaaggttgta aaagagtcgc ttaaccatac
     9661 gtaccaagaa acgttaaaaa atagccgttt ccattcgcta aaggtctcgt tttgataaaa
     9721 agaagctgag aatgtaagtt atatgaaagt ggagaacaaa atatttcgtt tcattcaaca
     9781 agtattctct aggtattttt cctgtttcaa aaaaagaaaa caaaatagtt aaaatattct
     9841 tgcatttcaa aaaaaacagg tgtattatta cgtcaacaaa taaacataca gcaaagcgtc
     9901 gaggaaaaca gtaggctatt caacgattta ttcccagtga gtcatgtaag tgagaaatga
     9961 caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta gatcattgtg
    10021 gctgcctcca ttatcaggct agcggataac tttatcaaaa agcgtccgtg aatgaagaag
    10081 cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt cccaagtgga
    10141 aaagggcttt tttttatttt aaggtggtgg ttttcatgga atctgatatt agtggcatta
    10201 ctaattgctg caacgtttat atcgtcaatc aaaatgataa ttattggagg aaaaatttat
    10261 ggaagaaaca aaaacttatt catggaaaca actattgatg gtcagttcac ttatcttcgg
    10321 gatgttcttc gggtcaggta atctaatctt tcctgtccac ttaggtcaaa tggctggcgc
    10381 aaactgggtc agtgctggcg taggatttgc tatctcaggt gctcttttcc cattactagc
    10441 tatcttagct gtagtagtga caaaaagtga tggtctgtat gacttagcta aacctgtcgg
    10501 aaaatggtat gctgccttgt tcttaatcct tgttcatttg acgatcggac catttttcgg
    10561 tacaccacgg acagctgcta ctgcttttga aatggctgct aaaccatttt tgcctgaaaa
    10621 atataattca ttaggtatgt ttttattctc tgctcttttc tttttacttg cttatttcct
    10681 tactgtccat ccaacacggt tggtcaaata cgtcggaaag tatctaaact ccttgtttct
    10741 tgcattatta gcaatcattt ttgtcgtttc tttattccat ccaatgggaa atctaaatca
    10801 atctgctgca gatacttatc aaacaaacgc tttaacaaat ggtgtcctag aaggttataa
    10861 cacggttgat gcagtagctt tgcttgcctt aagtgtcact tttgtccatg cagtccgtga
    10921 ccttggtttc aaagataaaa aagtcacaga attaacggca aaagctggta gcctgagcgt
    10981 tgcgctagaa gtcatgatct attttggact tgttcttctc ggtgcgatga gtttgaataa
    11041 gatgtcttta tcagaaaatg gcggaactgc actttctcag atcactaatt tttatttagg
    11101 taacttcggc gctagcttct taggagtgat ggtcacatta ggtgtcttca ctaccgctat
    11161 gggactcgtt gtttcttttg cgcaagattt ccataaactg ttccctaaag tcagttatat
    11221 gacatggctg cggttaacga ctttcgtctc ttttgtcgtt gcaaatgctg gtttagacaa
    11281 tatcatccaa tggtctttac cagtattgat gcttttgtat ccattgtcgc ttgcgttgat
    11341 cttgctttcc ttaacagcaa aatttttcca aaagacacca tttgtctatc aagtaacgat
    11401 gttgtttgct gctgttcctg ccgtattgga tatgctggca aattcgcctg ctttagtgag
    11461 tcaacaaaga gttgtagcaa gcatgctcga attttatcat catcacgttc cttttgctgc
    11521 attaggactt ggctggatgg tcccaacgtt acttggttac gctggcagcc tactgtttta
    11581 ttacgcctat cgcctatcag ggtataaaca agaagcaaat gaactaccag aagaataacc
    11641 aaatcaaaca caatttaaca taagaaatat taaacaggaa aaaaccagct agatgaaaga
    11701 atcctattct ttcgtctagc tattttttag ttctcttttt tacgcgcaca aaaaaactgc
    11761 ggacaatgat tgtccgcagt ttaaatgggc tctataatat ctagtgttgg tgacgccttc
    11821 tgttgccaat actttttctt ttttctaata aacgcaaaca gacaccttga aatcctttag
    11881 aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc agagtttacc
    11941 aaagagttac tagatattct agacctaaat cttatctttc atgaagatgc ctttcgcaaa
    12001 gagcgaatca atgacgaaac atgttttgtt tttgatggca cattgaccta tcaaccagag
    12061 gaatgcttcc attgtcacta tcaaaataag caaacgatca ttaaat
//
LOCUS       DBKOIJ010000082        12023 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000082 DBKOIJ010000000
VERSION     DBKOIJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.084"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(15..872)
                     /locus_tag="ACV5NJ_001731"
     CDS             complement(15..872)
                     /locus_tag="ACV5NJ_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHY6485818.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            992..1903
                     /locus_tag="ACV5NJ_001732"
     CDS             992..1903
                     /locus_tag="ACV5NJ_001732"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HHY6485819.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            1910..3556
                     /gene="mdcA"
                     /locus_tag="ACV5NJ_001733"
     CDS             1910..3556
                     /gene="mdcA"
                     /locus_tag="ACV5NJ_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HHY6485820.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            3658..3957
                     /locus_tag="ACV5NJ_001734"
     CDS             3658..3957
                     /locus_tag="ACV5NJ_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HHY6485821.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            3947..5623
                     /gene="mdcD"
                     /locus_tag="ACV5NJ_001735"
     CDS             3947..5623
                     /gene="mdcD"
                     /locus_tag="ACV5NJ_001735"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HHY6485822.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            5627..6220
                     /locus_tag="ACV5NJ_001736"
     CDS             5627..6220
                     /locus_tag="ACV5NJ_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HHY6485823.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            6244..7320
                     /locus_tag="ACV5NJ_001737"
     CDS             6244..7320
                     /locus_tag="ACV5NJ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HHY6485824.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            7345..8214
                     /gene="mdcB"
                     /locus_tag="ACV5NJ_001738"
     CDS             7345..8214
                     /gene="mdcB"
                     /locus_tag="ACV5NJ_001738"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HHY6485825.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            8325..9089
                     /locus_tag="ACV5NJ_001739"
     CDS             8325..9089
                     /locus_tag="ACV5NJ_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HHY6485826.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            9578..11671
                     /locus_tag="ACV5NJ_001740"
     CDS             9578..11671
                     /locus_tag="ACV5NJ_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HHY6485827.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            11696..11998
                     /locus_tag="ACV5NJ_001741"
     CDS             11696..11998
                     /locus_tag="ACV5NJ_001741"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6485828.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         3951 a         2119 c         2572 g         3381 t
ORIGIN      
        1 cagctctttt ttttttattt tcctaaataa aattcccgca tcgtttttgc aatcattttg
       61 atcaaaggat gatcttctgt gaagctagaa gataaataaa ttgttcgtgt caagggcaaa
      121 tctttgattt cccgcaagac gaaaggatca tttttatcgt aagaccaaga aacttttggg
      181 aaaaagctaa cagctaatcc ttgcttgaca atagaacgta atgtcgcagg atcatctgtc
      241 gtcgaactga tcgtcaattc gatacttttc gcttcaagtg ctgcatccag tgtatgacgg
      301 aaggcgttgc tctttgacag catcaagatg ggggtgcgtt ggatatcttc taattgcaga
      361 tgttcttttt tagcaagagg atgtttctta ggaatggcga cataaacagg ttctttcatg
      421 ataggaatat tggtcatgcc aggcttcatc tccgaagtga tgtacagtac aggatcggta
      481 atatcatctg gtaaatgatg ttgcatgatc gttaattgga tatcaggatg cttttccata
      541 aaaattcgga ccaagttcgg aatagaaaca gaggctacct ctaaatacaa tatgacggaa
      601 gtaacgatct tctcattgac cgaagacaat aatttgattc cttcttctaa tgtgtctaat
      661 gtcttgactg catatggata aaattcttct gcataaacgg tcgtctcaat gtttctgccg
      721 ctatggtaaa acaaagggac acctaattcg ttttccaaat gtttgatcgc acggctcaag
      781 gcaggttgag aaataaataa ttctttggct gcttgggtaa cattttttgt ttcgaatact
      841 tttttaaaat actgcagatg aagcaatttc ataattatcc atcccatcca tacctttttg
      901 ttatggtttt ttcttttttt ggcatttttt attatagcag aaattgacta agatagcagt
      961 ggaaaaagta agtgctgagg aggcgaggct catgagtaca ggtttcttat ttcctggaca
     1021 aggcagtcag aagttaggga tgttagcgga tatccctgaa atgtacatgg aacgtttaga
     1081 aaatgtgacg gggtatcggt tagttgataa agaagaaaac tatcaagata cggtttttat
     1141 ccagctagca ttactgacaa aagcagtatt ttatttagat gaaatggaaa aaaacggtat
     1201 tcagcctgat attgcagcag gacattcgat tggtgctttt tgtgcagctg ttgcttgtcg
     1261 atctttgaga tttgagaatg cagcggaact agtctatcac cgcgcagtct taatgaaaga
     1321 agcttatcca agcggttatg caatgggcgt agtagtaggt gtgacgagaa gcgaagctga
     1381 ggaaattgta gcaaaaacat ttgatcctga ccatccagtt tatctgtcaa atgaaaattg
     1441 tccgatgcaa catacgttat ctgggagtat cgaaggttta tccgttacgt tggcagaagc
     1501 caaacggcac caagcgcaaa cagcgaagct gctgaaagtc cctgtgccat ctcactgtct
     1561 cttgatgaat gatacggtca agaaattcaa gccgtatatg cagtctgtcc atctatctga
     1621 tgctagatgt ttgtatataa aaaatacgga tggaagagcc actcaggacg cagaagagat
     1681 tcgtaaagat ttgttggaaa acctagcttt ccctgtgcag tggaaccaaa tgatggactt
     1741 ggcaaaagaa ttaggaatgg atctgacgat tgaatttccc cccggcaata ctttgacaaa
     1801 gctgattcat acaaaatttg gtgtagataa agggatccgt acgatcaatc tagaccaaca
     1861 tggtattgat gacgccacgt ttctatatca aaaatggaga tgactaatta tggaaaaacc
     1921 aaaccgcaac tgggcttcta aacgcgaaaa aaaagcgcag cgattggaaa aaatcagcaa
     1981 cttgctcaat ggcaagtatg tagaaactga aaaaatcgta gaagtaatgg aaaaactgat
     2041 tgctcccggc gacaaagttg tattagaagg cgacaatcaa aaacaagcaa gttttctttc
     2101 agaaagtctt gaaaaagtag atgcagaaaa agtgaatgat ctgcatttga tcatgtctag
     2161 tatctcacga ccagaacatt tgaatatttt tgaaaaagga atcgcaagca agatcgattt
     2221 ctcttatgct ggtgcacaaa gcttgcgtgt cgcccaaatg attgaagacg gcacaatgaa
     2281 attaggtgat atccatactt atttggaact ttatggacgc ttattcatcg atttgattcc
     2341 aaatgttgtt ttagtagctg cagataaagc agataaagac ggcaacttgt acacaggttt
     2401 caatacggaa gaaacaccaa caatcatcga agctgcagct ttcaaagatg gtatcgtgat
     2461 cgttcaagtt aacgaagttg tcgattcttt accacgtgta gatatccctg gtgactgggt
     2521 agacgttgtc gtgaaagcag ataaacctta tcaattagaa gctttattca cacgagatcc
     2581 tcaaaacatc acagaattgc aaattctaat ggcaatgatg gcaatcaaag gaatctacgc
     2641 agaacacggc gtacaatctt tgaatcacgg tattggattc aatacagcag ctatcgaatt
     2701 gttactgcca acttatggtg aatcactagg gttaaaaggg aaaatcgcga agaactgggt
     2761 attgaaccca catccaacga tgattccagc catcgaatca ggttgggttg aaagcatcca
     2821 tagttttggt ggcgaagttg gtatggaaaa atatattgct gctcgcccag atgtcttttt
     2881 tgtcggtaaa gacggcacga tgcgcagtaa ccgaacatta tcacaagctg caggacaata
     2941 tgccatcgat atgtttatcg gttcaacctt gcaattagat tacgaaggaa actcttctac
     3001 cgtaacaaaa ggacgtttat ctggttttgg cggtgcgcct aacatgggac ataatcctgg
     3061 cggtcgtcgt cattctacac cagcatggca atcacttcgc gacaatgatg atccattagg
     3121 gaaaggccaa aaattagttg ttcaaatggt tgaaacatat ggttcaaata aaaaaccagt
     3181 ctttgtcgaa gaattagatg cactagctgt tcaaaaacaa gcaggcttag ccaatgcacc
     3241 agtgatgatt tatagcgaag atactacaca tatcgtgaca gaagaaggaa ttgcttattt
     3301 gtacaaagca aaaacaaaag aagaacgaca agcagcaatt gcagcaattg gcggtgttac
     3361 acctcttgga atgactagca cgaaagaatc actagatagc ttgcgtgcag ccggcatcgt
     3421 gaaattacca gaagatctag gcatcaaacg tagtgaagcg aaacgttcat tattagctgc
     3481 acaaaccatc gatgatttga tcgaatggtc agatggcctg tatgaaccac caatgaaatt
     3541 cagaacttgg tcataaaatt ggcgagaaaa accttttgtc gccatttacc cagatggaag
     3601 aagaaagacg aataagcaga acgtgtatgc tctgttttac aaaaagggga gagatccatg
     3661 gaacaattag cttataaata tccaacgaaa cagccgatca ataagaaaat ccatgtagga
     3721 gtggtcggtt caggagattt ggaaatcttg atgtttccaa cagaaaaaac agaatctact
     3781 gtaaaaattt gtacaggaag cgatggcttc ggcgaagtat ggaacaatgt attaacgaga
     3841 ttttttgatc gttatccaat ttcagcagat atcgtgatca atgacttcgg tgctacacca
     3901 ggagttgtgt acctacgatt gacgcaagca atggaggaac tttcagatga aaaatagttt
     3961 tgtagaatta aacgccagag aacgagtgaa cgctctactt gacaatggac gtgaattgat
     4021 tgatccattc gatcaaatca tcgcgccaag tttaccagca caaggaatcg tccctgaaag
     4081 tgatgacggg atcgttgtca gccgtggcac gatcaatggc aaacaagcag tagtaatcgc
     4141 tatggaagga aaattccaag gtggcggtat cggtgaagtt tccggtgcaa aaatcgttgc
     4201 tgcacttgaa catgtgttag aagaaaataa aaaaggaaat gagatctttc cagtcattgt
     4261 actagataca ggtggtgttc gtttgcaaga agcaaactac ggactgttat ctatttcaga
     4321 aatcggaaat gtaatcattg ctctaaaaca atatgtacca gtcatcggtt tgattccagg
     4381 acgtgtagga tcatttggcg gtatgtcgat tacttcagca ttaatgagtt atttaatcgc
     4441 aacaaaaaaa gcgagagtcg gtttgaatgg accagaagtt attgaacaag aagcaggcgt
     4501 tcgagaattt gattctagtg acaaagactt gatttggaat acattaggat ctcgtcaacg
     4561 ggtagaatca aaaatcattg atgaattagt gactgatagt gtagaagcaa tcaaagctgc
     4621 tgtcatcgca gcaatggacg aacgaaaaga cacgcatcgc agtgaaagct cagcatttta
     4681 tttatcatta cttgattctt tagatctttc aaaaccaatg gccattgacg attacaatga
     4741 agcaattgct aaccatcaag caaaatcaat tgacttacca gaagtagcag aaggaaatga
     4801 accagctgca gcaagtgttg gttacgattg gtttgcggca ctaactggca tgaaaaatcc
     4861 aacttcttct attagtactg tgtattatgg taaatcaagt aaattcatag aagaagccat
     4921 cgtgacatca atcgtacctg atcctaaaaa cccgatgtac cgtgtacgaa acggtgaagt
     4981 tggtttagtt gaaggtttcc gcatgggaca aattttggat cacgtttacg aagaagataa
     5041 agacaaaaaa gtgaaacggc caatcatcgt tgtgatcgat gtaccaagcc aagcatacgg
     5101 ctataacgaa gaattgatcg gtatccatgt ggctttggct aatagtgctg ctgcttatgc
     5161 aaaactccgt caagctggac atccagttat tggattgatt gcaggaaatg ctatttcagg
     5221 tgcattttta gcacatggat tacaatcaag ccgcttgatt gctttaaaca ataaagaaat
     5281 taccgtccaa gcgatgtcta aagaaagtgc tgcacgtgtg acaaaacgtt cggttgctga
     5341 aatcgaagca gctgctgaaa aagttccagc tatcgcttac gatatcaata attatacaaa
     5401 actaggagcg atctatcatt tcctagaaga tatcaccgac caagctgctt cagaacaaaa
     5461 tgtcagcaaa gtcatcgaag caatcaatga agccattcaa gatgtccgca atacaaatga
     5521 cacgatgctg acaaatcgat acacaaacga aacagccaaa acattcggac gtgtcgaaac
     5581 aaataaagtc cgtgccaaaa tggacgaaga atgggatgca taaaaaatga ttcaagcaca
     5641 tgatattgtc ctttttgata aacatgaaat agaagggttc accttgcctg aatgggtcgt
     5701 tctaactgaa aagatgttcg gaacagttcg tcgtgcgtcg acggatcacg cagaaatact
     5761 accgattggt ttacgtggac ctgcccgcaa tcagagatgg tcaacagaaa tctcaaaaca
     5821 tgccgtcaaa caagtcattc atccgtggga catcatcgaa caagaatcgt atagacaaga
     5881 gaaaatcatg gattatcctg tttatcagca atttcatgct gctagaaaca tattatcagg
     5941 atgtaaatgg ggaatcggcg gcagcttagg atttgaatta tcgactggga ttccggcagt
     6001 aaaagaaacc agtgattttg atttgctgct gtacgcaaat tcaccgattg aattgccaat
     6061 tcaggtaatt caatcgcatc ctgctttttt tgagcaattc gatacccaag tagtgactcg
     6121 gcaaggcggt ttttcactaa aagagtattt gagagagcca gagaaaaaaa ttctattgaa
     6181 aacagtcgaa ggacctaaat taaccaaaga actttggtaa taaagaaaaa gagagagaaa
     6241 gagatgtttg aaaacaaaaa aatcaaaatc aacgtaagtg acttccagaa agtatcagct
     6301 gtaacggctg ttatgatgca aacgatattg agctttgctt tggcaaacag ccacagcgca
     6361 acaaatacag cttttcttgg taccttttac gtcatcgtta aatacacagc accaatgttt
     6421 atttttgcaa ttgtttataa tatggtaaaa acaagtcagc atttgtctta ttgggaattt
     6481 ttgaaagaaa agttttttga actagtcgtt ccgtatcttc tatggacgac ggcttatctt
     6541 tggctatttc cagcagtcca acaacaaact ccgtatacgg atactgcaag ctttttactg
     6601 aaatatgtca caggtgatgg agcgcctcat ttatggtata ccgttatgat gctgcagatc
     6661 caattgttca tgccgttttt tgtttggctc ggttacaaag tatttgctaa taaaaaatat
     6721 gtatggccag tattgattgg ttcaacgatt ttatatgtcg catggtatct gttctatcaa
     6781 acacaagtat tgactgggcc ttatcatgac agctggtact tattagatcg gtttgttgct
     6841 agtttcatga tttatggggt agccgcattt gtttatcacg aaaaagtcta tcaatatttg
     6901 gacagagtac gttatctttt cttacctgtt gccttagtta tcgcgttctt ttctgtccgt
     6961 tcattgctcg cacatccagg agacttgagc tttgcgaatg caccttactt gaatacgatc
     7021 caaagtttat acagtttagt catcatcttt gctgtcttca taggcgcttc caaaatgatt
     7081 gtaaacgatt cgccaaaatt gccattgttc aaatggttgt ctgtttatgc ttatcgtacg
     7141 tatttagcca atgtatttgt tttccaagta ctgttattat tgtttaaaga tagttggctg
     7201 cagttacctt caggagtcat gattcttgta gcttatctaa tgacggcaag ttgtgcattt
     7261 gctttaagct ggttacttca tatcatttgg gtagctatca aaaaaggttt cagcaagtaa
     7321 tagtaggaag aaggagtgag tgtcatgata aatgacgaac gaaaaaaagc agtgactcag
     7381 caactcgctg cgttggtaga ggaagcattg atcgcagaag taacattaag tccaaaacct
     7441 ggattagtgg atgcactgga tgcgggtgca cattccgata tggactatgc tttatttctt
     7501 aagtctgcta agacgctaac tccttttttt gaagaaatgg ctcaaatagc atggcatcat
     7561 gctattgatc aggagttaag agaaagaatt gctgagattg ggcgaaaagc ggaaaaggca
     7621 atgctgattg ctacaaatgg catcaatacc cataaaggag cgatttgggc aatggggcta
     7681 ttgatcagtg tcattgcaca aaaaatcagt atggaacagc ctttttcttt atcgaaaata
     7741 cttgatgatg tcagcaagct tgctgctttt cctgatacaa aatatcgtca taaaaaaacc
     7801 actcatggtc atagggtgaa gcaaaaatat ggcgtaaatg gtgcatatga agaagcgata
     7861 ttagggtatc cgcatatcag gcttgcaatt caagcagcta aggagctaga taatgattca
     7921 tatgtacaga aacaaatgca tatgttgtta gttttgatga gttcgctaga tgacacatgc
     7981 gtattgcatc gcagtgatca gaaaacgtta gtggaaatgc aaaaacttgc tcatgctgct
     8041 agtcaaagtg ctttaccaaa tcgtccattt acagcactgt tgcacttttg tcaggaaaac
     8101 caaatctctc ctggaggaag cgcagatctc ttgtctgcaa gcttgttcat gctgaagata
     8161 gaggaattat ggactgccga cacatacatt tgtccgatgt tactcaatga ctaaataaga
     8221 caaaattgct aaaaaaacag tttatgcatc tggttattga ctcttccttt tagcaaggct
     8281 atcataaaaa tagttttttg taataaaaga taacgaggaa gacaatgaaa aaaatcaatt
     8341 atttagcaat cattttgtta gtgggcctat gtttgtttgc tctgccgacc caaggattcg
     8401 ctgtcgcggg tggtcatggt ggaggtacat ctagcggtcg aagttcgggc agaggttcat
     8461 ttggtggtcg ttcatatggg ggcagttatt ttggcagtcg ttcttacggc agagattctt
     8521 atgggtatta tggtccaggt acaggtcctt ctagttatga gcaaacggga tttcttgcat
     8581 tagctgtttt gtcagtagga ggggtttctg ctttttctat cgtcaagaat aaaagacaaa
     8641 agaaaaggct gaaagcggag atattgtccc gcatgtcagg aacggctaaa gaaaagaaag
     8701 cacagtttct ggaaatccaa aagatattca tgaagatcca atccgcttgg gaagaagaaa
     8761 ctcctgaaaa agccagaagc tgttatacag acaagctgtt tgccgaacat cagaaagttc
     8821 tgcaaaaaaa caaagcagac ggtgtaagaa atcatactaa aaaaataact attcaggaag
     8881 taggaaatta tcgacagatc cacgaaaata gttttgccat ccgaattgat ttcagatgct
     8941 gtgattattc agtcgatcga aaagatgggc gattgatctc aggaagtaag acacaacgac
     9001 agtatttttc tcagatatgg tattttgatt tttctgagaa agaaaaaaga tggcaagccg
     9061 attttattca gccgattatg ctggattgac aggaaatatt tatgaacgga gttgtgacaa
     9121 aagccttgtc gcagctcctt tttctgaata aacggtgtgc cagagatagc ctatcggcaa
     9181 taaggcacga ttttgtaaaa tatgggaagc gatttttcaa aatcgcttct tatcgctcaa
     9241 ggctgaacat ctctgtcaca acctctccac taaatggtgt ttaaaagctt gcatctgcga
     9301 taataagaac aaccaaacga aaaataggaa gcactatttt agtttagttg ttcttattac
     9361 ttgatgcagg acagcttttt cacaacctct tttttgtata gatgggcaaa atttttgtaa
     9421 taataggcgt atgtcttttt cgttgaggag atgaaaaagc atatggctta ttttttgttt
     9481 gctttgctta ctttctttcc acttcaagtg gcagaaatat ctacttttta ttatgtaagc
     9541 ggaaccatat acttgaaatc agaaggagat catttctatg aaaacaagaa caattgaaat
     9601 acttcacaga atcatgacag cccaatatcc agtaaaagca aaacatctag caagtgagtt
     9661 caatgtcagt cagcggacga tccgtcagga agttcttgaa gcgaatatct ggttgagcag
     9721 taaaaagctt ccggagatcc gtacaattcg caacaaaggt tttttgttga agctggaaaa
     9781 ggaaaagcaa gctcgattag aaactgaaat aaaagatgtg aaagacggct ttttgaatcg
     9841 gcgcgaacgt atttttgatc tagttttagc cgttgcttac agccgaatcc cgatccatct
     9901 aaacaaaaaa gaagaagaat ttcaaatctc taaaagtaca ttagatgaag atatgagacg
     9961 gttacgagca acactggtca gttatgggat agagatcgtc agttatggaa agcaaggact
    10021 ggtttataaa ggttcagaac gatctatccg tacgatgatc tatgatttta tcaatcagaa
    10081 tctgggaatg accgacttct ttatgaatca agatgcacaa ttaacaccgg ctcaaaaaat
    10141 ctttctccat tattttcctt tggtggaaat caaaaaaata caggagatct attccaataa
    10201 gttgttgaaa caagaagatg atatttataa aaaacaaatc ctgcttttta cattgatctg
    10261 gatccagcga atcaggaaac atgaatttat ctctgccatt aattggaaaa atacagaaat
    10321 aaaagacagt agcttttctg catttattga ccacatgatc gaggaatttc aattggacaa
    10381 tgtcccgcaa gtagaaagaa attatcttcg tttcactatt gagacgttca atgctagaga
    10441 tatctccaat tcaatcgagt gggtacaagc tcaattacta acgatccaat tgatccaatt
    10501 tgtagagaac gagacacacg tacctttcca tctaaaggaa gaagctttat tcgaaaatct
    10561 ctataagcac atggctgcac tgattgtaag aatcaagaat catatccaag tcatgaatcc
    10621 attgaaagaa aacatccgaa caaattattt cccaatctac cgcgcagtca ctcattttgt
    10681 tcctacgatc gaagaagtaa ttggtggcaa gatactggag gacgagatag cttttcttgt
    10741 catccacttt tcgacgatcg ccagtgtaat caagcgagac ttgaattatg tttataaatc
    10801 agtcgttgtc tgtaaccatg ggatggctac tggaaatctg ttagcagaga atctgaaaga
    10861 aaagtttcct cagatcgaga ttactgcagt tttaagttca aaagaaatcg aattagtaga
    10921 taaattggat gtcaatctgg ttttcagcac ctttcaattg aattatcgaa ataagccgct
    10981 tttagttgtc gatccgattt taactgagac gaatatcttg gttatagaag actttttgaa
    11041 aagaaatgaa gaactacagc ggttagagcc taacagtcat gaatcgacgg agttattcac
    11101 tcaaataata gatgtcatga aagaaagtgg tggccgagta gatcgaatca tctatggcaa
    11161 gttagaaaaa ttattcgaaa caaacaattt agaaatcaac aaaagggaga tccagcctat
    11221 gttaaaagat attttgacgg acagcaatat catgatcaaa gaaaaagcca ctacttggga
    11281 ggaatcgatt gaactagctg ctagaccatt ggttaaagaa aacattgtcg aagagcgcta
    11341 tgtcgaagcg atgattcgtg cagtgaatga ttacggaccg tatatcgtga ttggcaaaca
    11401 catggcacta gcccatgccc gaccagaaga tggggtcaat aaattaggag tcagcgtagc
    11461 tacgattgag caaccaatcg atttcggtaa tcctgaaatg gatccagtaa agattatttt
    11521 ttgtttggca gcagtagact cctactcgca tctgaacatc atgaaagaac taattgaatt
    11581 gatcaacaac gaagagaagt tggatcgatt gattgaatgc acaaatatcg aagatttcaa
    11641 acaactgctt ttttcagtag ttgagaaata ataaatgata cgggggagaa caaagatgaa
    11701 caaattgaac attttattcg tatgcggtgc aggtttagga agtagttttg cagcacagat
    11761 gtctgctgag gatgttttga acaaattagg agtagaagcc aaactggacc attgtgatat
    11821 ttcttccgca gtttccatga aaccagatgt gatcatcaca gcagaaaatt ttcgttcgca
    11881 gttcgaaaag ttcgcagttg atcctaaaac ggcgatgatc tatttgaaaa atattgtttc
    11941 aaaaaatgaa attgaagaaa aaatcacccc agttctgcaa gataagggag ttatctaaat
    12001 acaaaaagaa aagagggtaa aaa
//
LOCUS       DBKOIJ010000083        11612 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000083 DBKOIJ010000000
VERSION     DBKOIJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.085"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            237..1877
                     /locus_tag="ACV5NJ_001742"
     CDS             237..1877
                     /locus_tag="ACV5NJ_001742"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HHY6485829.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACV5NJ_001743"
     CDS             2031..3353
                     /locus_tag="ACV5NJ_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HHY6485830.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACV5NJ_001744"
     CDS             complement(3415..3846)
                     /locus_tag="ACV5NJ_001744"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6485831.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACV5NJ_001745"
     CDS             complement(3910..4746)
                     /locus_tag="ACV5NJ_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HHY6485832.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACV5NJ_001746"
     CDS             complement(4936..5238)
                     /locus_tag="ACV5NJ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HHY6485833.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACV5NJ_001747"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACV5NJ_001747"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HHY6485834.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACV5NJ_001748"
     CDS             complement(5692..5958)
                     /locus_tag="ACV5NJ_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HHY6485835.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACV5NJ_001749"
     CDS             complement(6212..6937)
                     /locus_tag="ACV5NJ_001749"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HHY6485836.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACV5NJ_001750"
     tRNA            complement(7303..7373)
                     /locus_tag="ACV5NJ_001750"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACV5NJ_001751"
     CDS             7490..8242
                     /locus_tag="ACV5NJ_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HHY6485837.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACV5NJ_001752"
     CDS             8258..8737
                     /locus_tag="ACV5NJ_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HHY6485838.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACV5NJ_001753"
     CDS             complement(8988..10568)
                     /locus_tag="ACV5NJ_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HHY6485839.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACV5NJ_001754"
     CDS             complement(10561..11304)
                     /locus_tag="ACV5NJ_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485840.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..>11612)
                     /locus_tag="ACV5NJ_001755"
     CDS             complement(11480..>11612)
                     /locus_tag="ACV5NJ_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HHY6485841.1"
                     /translation="NITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         3527 a         2316 c         2061 g         3708 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tt
//
LOCUS       DBKOIJ010000084        11610 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000084 DBKOIJ010000000
VERSION     DBKOIJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.086"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(6..347)
                     /locus_tag="ACV5NJ_001756"
     CDS             complement(6..347)
                     /locus_tag="ACV5NJ_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HHY6485842.1"
                     /translation="MIKKMIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFS
                     DSQQKQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDES
                     ADTSYKLLSEK"
     gene            512..1240
                     /gene="sfsA"
                     /locus_tag="ACV5NJ_001757"
     CDS             512..1240
                     /gene="sfsA"
                     /locus_tag="ACV5NJ_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HHY6485843.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1256..2269
                     /locus_tag="ACV5NJ_001758"
     CDS             1256..2269
                     /locus_tag="ACV5NJ_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHY6485844.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2269..3546
                     /locus_tag="ACV5NJ_001759"
     CDS             2269..3546
                     /locus_tag="ACV5NJ_001759"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HHY6485845.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3609..6311
                     /locus_tag="ACV5NJ_001760"
     CDS             3609..6311
                     /locus_tag="ACV5NJ_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HHY6485846.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6463..6780
                     /locus_tag="ACV5NJ_001761"
     CDS             6463..6780
                     /locus_tag="ACV5NJ_001761"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6485847.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6810..7130
                     /locus_tag="ACV5NJ_001762"
     CDS             6810..7130
                     /locus_tag="ACV5NJ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HHY6485848.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7238..8698
                     /locus_tag="ACV5NJ_001763"
     CDS             7238..8698
                     /locus_tag="ACV5NJ_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHY6485849.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8766..8987
                     /locus_tag="ACV5NJ_001764"
     CDS             8766..8987
                     /locus_tag="ACV5NJ_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485850.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9018..9200
                     /locus_tag="ACV5NJ_001765"
     CDS             9018..9200
                     /locus_tag="ACV5NJ_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HHY6485851.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9200..9613
                     /locus_tag="ACV5NJ_001766"
     CDS             9200..9613
                     /locus_tag="ACV5NJ_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HHY6485852.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9736..10917
                     /locus_tag="ACV5NJ_001767"
     CDS             9736..10917
                     /locus_tag="ACV5NJ_001767"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6485853.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10988..11152)
                     /locus_tag="ACV5NJ_001768"
     CDS             complement(10988..11152)
                     /locus_tag="ACV5NJ_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485854.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11306..11379
                     /locus_tag="ACV5NJ_001769"
     tRNA            11306..11379
                     /locus_tag="ACV5NJ_001769"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11340..11342,aa:Arg,seq:cct)
BASE COUNT         3813 a         1961 c         2446 g         3390 t
ORIGIN      
        1 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
       61 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
      121 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
      181 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
      241 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
      301 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
      361 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
      421 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
      481 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
      541 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
      601 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
      661 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
      721 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
      781 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
      841 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
      901 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
      961 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
     1021 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
     1081 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
     1141 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
     1201 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
     1261 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
     1321 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
     1381 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
     1441 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
     1501 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
     1561 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
     1621 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
     1681 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
     1741 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg
     1801 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
     1861 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
     1921 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
     1981 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
     2041 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
     2101 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
     2161 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
     2221 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
     2281 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
     2341 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
     2401 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
     2461 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
     2521 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
     2581 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
     2641 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
     2701 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
     2761 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
     2821 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
     2881 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
     2941 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
     3001 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
     3061 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
     3121 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
     3181 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
     3241 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
     3301 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
     3361 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
     3421 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
     3481 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
     3541 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
     3601 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
     3661 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
     3721 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
     3781 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
     3841 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
     3901 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
     3961 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
     4021 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgatcaaaat ccgcaactgc
     4081 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
     4141 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
     4201 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
     4261 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
     4321 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
     4381 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
     4441 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
     4501 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
     4561 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
     4621 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
     4681 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
     4741 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
     4801 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
     4861 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
     4921 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
     4981 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
     5041 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
     5101 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
     5161 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
     5221 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
     5281 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
     5341 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
     5401 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
     5461 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
     5521 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
     5581 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
     5641 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
     5701 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
     5761 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
     5821 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
     5881 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
     5941 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
     6001 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
     6061 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
     6121 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
     6181 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
     6241 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
     6301 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
     6361 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
     6421 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
     6481 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
     6541 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
     6601 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
     6661 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
     6721 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
     6781 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
     6841 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
     6901 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
     6961 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
     7021 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
     7081 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
     7141 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
     7201 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
     7261 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
     7321 gcgtgatgct tttatcggca tactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
     7381 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
     7441 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
     7501 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
     7561 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
     7621 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
     7681 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
     7741 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
     7801 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
     7861 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
     7921 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
     7981 gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag gattatctca
     8041 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
     8101 tcatggatca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
     8161 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
     8221 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
     8281 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
     8341 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
     8401 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
     8461 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
     8521 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
     8581 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
     8641 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
     8701 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
     8761 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
     8821 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
     8881 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
     8941 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
     9001 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
     9061 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
     9121 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
     9181 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
     9241 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
     9301 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
     9361 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
     9421 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
     9481 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
     9541 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
     9601 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
     9661 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
     9721 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
     9781 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
     9841 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
     9901 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
     9961 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    10021 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    10081 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    10141 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    10201 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    10261 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    10321 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    10381 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
    10441 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
    10501 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
    10561 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
    10621 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
    10681 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
    10741 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
    10801 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
    10861 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
    10921 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
    10981 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
    11041 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
    11101 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
    11161 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
    11221 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
    11281 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
    11341 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct
    11401 ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt
    11461 ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga
    11521 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
    11581 acgctaagta tatttactcc ttgataaagt
//
LOCUS       DBKOIJ010000085        11558 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000085 DBKOIJ010000000
VERSION     DBKOIJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.087"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(366..1484)
                     /locus_tag="ACV5NJ_001770"
     CDS             complement(366..1484)
                     /locus_tag="ACV5NJ_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485855.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1717..1839
                     /locus_tag="ACV5NJ_001771"
     CDS             1717..1839
                     /locus_tag="ACV5NJ_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485856.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1955..3262
                     /locus_tag="ACV5NJ_001772"
     CDS             1955..3262
                     /locus_tag="ACV5NJ_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HHY6485857.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="ACV5NJ_001773"
     CDS             3381..4580
                     /locus_tag="ACV5NJ_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HHY6485858.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="ACV5NJ_001774"
     CDS             complement(4607..5875)
                     /locus_tag="ACV5NJ_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HHY6485859.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5930..5996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6038..6684)
                     /locus_tag="ACV5NJ_001775"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="ACV5NJ_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="ACV5NJ_001776"
     CDS             complement(7092..7595)
                     /locus_tag="ACV5NJ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HHY6485860.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="ACV5NJ_001777"
     CDS             complement(7718..8482)
                     /locus_tag="ACV5NJ_001777"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HHY6485861.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8590..8865
                     /locus_tag="ACV5NJ_001778"
     CDS             8590..8865
                     /locus_tag="ACV5NJ_001778"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HHY6485862.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9303..10253
                     /gene="yidC"
                     /locus_tag="ACV5NJ_001779"
     CDS             9303..10253
                     /gene="yidC"
                     /locus_tag="ACV5NJ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HHY6485863.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10432..10599)
                     /locus_tag="ACV5NJ_001780"
                     /pseudo
     CDS             complement(10432..10599)
                     /locus_tag="ACV5NJ_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="catalyzes the reversible reaction of
                     dihydroxyacetone phosphate with glyceraldehyde 3-phosphate
                     to produce tagatose 1,6-bisphosphate; in Streptococcus
                     pyogenes there are two paralogs of tagatose-bisphosphate
                     aldolase, encoded by lacD1 and lacD2; expression of lacD1
                     is highly regulated by environmental conditions while
                     lacD2 specializes in an efficient utilization of
                     carbohydrate sources; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            11437..>11558
                     /locus_tag="ACV5NJ_001781"
     CDS             11437..>11558
                     /locus_tag="ACV5NJ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6485864.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         3493 a         2423 c         2037 g         3605 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt cagcacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgctgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     7201 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     7801 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     7861 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     7921 ctgtgccata cactgtaaac cctttgtctt tcattaaaga aatcacttct gtcaaatctt
     7981 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     8041 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     8101 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     8161 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     8221 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     8281 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     8401 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     8521 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     8581 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     8641 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     8701 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     8761 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     8821 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     8881 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     8941 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     9001 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     9061 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     9121 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     9181 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     9241 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     9301 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     9361 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     9421 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     9481 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     9541 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     9601 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     9661 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     9721 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     9781 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     9841 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     9901 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     9961 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    10021 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    10081 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
    10141 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
    10201 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
    10261 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
    10321 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
    10381 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
    10441 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
    10501 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
    10561 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
    10621 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
    10681 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
    10741 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
    10801 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
    10861 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
    10921 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
    10981 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
    11041 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
    11101 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
    11161 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
    11221 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
    11281 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaataa
    11341 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
    11401 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
    11461 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
    11521 aaccgaatgg gacgtgctat acaaactatt tataatgt
//
LOCUS       DBKOIJ010000086        11486 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000086 DBKOIJ010000000
VERSION     DBKOIJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.088"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            196..1041
                     /locus_tag="ACV5NJ_001782"
     CDS             196..1041
                     /locus_tag="ACV5NJ_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHY6485865.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1232..2419
                     /locus_tag="ACV5NJ_001783"
     CDS             1232..2419
                     /locus_tag="ACV5NJ_001783"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HHY6485866.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2425..2781
                     /locus_tag="ACV5NJ_001784"
     CDS             2425..2781
                     /locus_tag="ACV5NJ_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HHY6485867.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2783..3121
                     /locus_tag="ACV5NJ_001785"
     CDS             2783..3121
                     /locus_tag="ACV5NJ_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HHY6485868.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    3197..3453
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3516..4550
                     /locus_tag="ACV5NJ_001786"
     CDS             3516..4550
                     /locus_tag="ACV5NJ_001786"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HHY6485869.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4543..5229
                     /locus_tag="ACV5NJ_001787"
     CDS             4543..5229
                     /locus_tag="ACV5NJ_001787"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HHY6485870.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5253..6101
                     /locus_tag="ACV5NJ_001788"
     CDS             5253..6101
                     /locus_tag="ACV5NJ_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HHY6485871.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6298..7071
                     /gene="sufC"
                     /locus_tag="ACV5NJ_001789"
     CDS             6298..7071
                     /gene="sufC"
                     /locus_tag="ACV5NJ_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HHY6485872.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            7084..8370
                     /gene="sufD"
                     /locus_tag="ACV5NJ_001790"
     CDS             7084..8370
                     /gene="sufD"
                     /locus_tag="ACV5NJ_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HHY6485873.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8367..9602
                     /locus_tag="ACV5NJ_001791"
     CDS             8367..9602
                     /locus_tag="ACV5NJ_001791"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HHY6485874.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9589..10059
                     /gene="sufU"
                     /locus_tag="ACV5NJ_001792"
     CDS             9589..10059
                     /gene="sufU"
                     /locus_tag="ACV5NJ_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HHY6485875.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            10064..11455
                     /gene="sufB"
                     /locus_tag="ACV5NJ_001793"
     CDS             10064..11455
                     /gene="sufB"
                     /locus_tag="ACV5NJ_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HHY6485876.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         3748 a         2171 c         2577 g         2990 t
ORIGIN      
        1 caagatgaac gaatacattg taaaatgaat aggaatgtat gattacgagc aagcagacag
       61 ctgattgtct tcatctaagt tactagttgg atcagcaact gttttgttgt acatttctat
      121 tatagatggt tttttgtata tttaaatcaa atagttttta tagtaattat cataaaaagt
      181 tatggaagtg gtgccatgtt taagttatta acgacttttc gagcagtata tgaaacaaga
      241 aatttttcca gagcagcaga agtactgttt ttatctcaac cagcagtctc gaatcaaatc
      301 aaacagttgg agcgggaatt gaatatcgat ctttttcaac gtaacggaag aaaagagatg
      361 cttccaacta agcaagccga cttattatat gaacggacac tggttttgtt agaagaatgg
      421 gaagaaactc agcaaaaatt gctcaatgaa caaaatgaag aagaagtatg tcggatcgtt
      481 gcttcccaca cctttgctgt ctatcttttg cctcagttga tgaaacatct gatcaaaagg
      541 tttcctaaag tgcattttga gatcgaaatc acgaattctc atgaatcatt agaacaagta
      601 gcgaaacatg aatccgattt tggttttatc gaaaagccat tagagacttc cggcgtacaa
      661 agatactctt tgatggaaga ccagctagtc atcgctgggg acccaaaaag caaattgtgg
      721 cttgtaagag aagcaaactc tggtgttttt cattacacgc agcgttattt tgaagagatg
      781 aatatccaag ataagaagat gatcgtgaaa agtaacgatg taattgttgc gttgctgaag
      841 gaaggaatcg gtcgctcgat tttgtctaag cgctctgttc cagaaaatgt gccaatgact
      901 ccgttgaatt tccatcgata tttttatttc atccaacgaa ccaatctggt ttctgatcag
      961 ctgctagaga ttgccgaaga gatccttcga tacaatgaaa gcacagaaac ttctcacagc
     1021 gaggagaatc tcagctggta gataattgca tgtatcaaaa aaagttctat gatacactaa
     1081 agataagcag taggcttttg tactgcattt gctcaagcaa ctgcacaaac ggaaagttaa
     1141 tttttcccaa atgtctatgg aaagactttc cattatctaa ctggaccgct ataataatca
     1201 gcagtatggt ttttaagaaa agggatgaaa catggaaaaa aaattaaaag cttcgaacga
     1261 taatcgtatt gattatggcg tgattttacc agtctttctg ttatgtttga ttggtctgct
     1321 ctcattatat gtagcactta gccatgatcc gaacaatcca gcagtcttga aaggcgtagg
     1381 gatgcaagtt gtctggtatt tagttggagc ggcagcaatc gttgtcatca tgcatttgaa
     1441 ttccaaatgg atctggaaac tgactcctta cctttatggt ttggggttag tggtaatggg
     1501 actcttgctc aaattctatg atcagaattt agctgcttca acagggtcta aaaactggtt
     1561 tcgattcggt gcgtttactt tccagccagc agagttgatg aagattgcgt acatcttgat
     1621 gatggcactg atcgtaacta aacataatac gcaagtaaaa gaacggacga tgaaatcgga
     1681 cttttggctg attgggaagc tcttgatcgt gacaataccg gttttagcat tgatcatggc
     1741 ccaagatgac tttggaacga tgcttgtctt tcttgcgatc tttggcggga ttttcctgat
     1801 gtctggaatc tcttggcgca tcatcgcacc tgtagtaatc ttggcagtag tcgttggagc
     1861 aggaacgatc ttcttggtta cgactgaagg cggaagagac ttgctttata aagttggatt
     1921 caaatcttat cagtttgcac gtattgattc atggctggac cctttccatg atacgtcagg
     1981 aatgagttat cagccggccc aaggactttt ggcaattggt accggtggct tgtttggtaa
     2041 aggattcaac gtaagcaata tctatgtgcc tgttcgtgag tccgatatga tttttaccgt
     2101 tatcggtgaa aacttcggct ttatcggtgg tgcattcgtc attttcttgt attttatttt
     2161 aatctatcgt atgattcggg tgtgtttcga tacgaataat gaattttacg cctatatcgc
     2221 aagcggtttg atcatgatgc tgctgttcca tgtctttgag aatattggag caaatatcgg
     2281 attacttcct ttaacaggga tcccattgcc tttcattagt cagggaggat cctcgatttt
     2341 agggaacatg atcggtatcg ggttgatatt atcgatgaga taccaaaatg aagcacctgc
     2401 aagatcttca aggaggtaag aaaattgtac accttttact ggtacccgaa atgttcaaca
     2461 tgcaaaaaag ccaaagcgtg gttagaccag catggcgtta gttatcaaac cgttgatatg
     2521 atcaaagaag taccgaaggc ctcaacgatt gagacttggc tagaaacgag tcaagtacca
     2581 atccgtcgcc tgttcaacac aagcgggatg aaatatcggg aacttggact gaaagatcaa
     2641 gtaggcgagt ttacggcttc aaaagcgagc caagtcttat cgacagatgg tatgctgatc
     2701 aagcgaccac tgatcttacg agaggaccag cttgttgcaa ttggattcaa tgaaaaaatt
     2761 tatgaaggag aactgatata aaatggcagg caagcatttg aagaaaaagg acaacctttg
     2821 gatcttattt aatggaaaag aatactgtgt tggtttgaca aatgaagcgc aagaagaact
     2881 tggagacatc acgtttgcca atctgccaaa agcaggacaa tcgctaaaaa aagatgaacc
     2941 tctgatcgaa gttgaagcgg aaaaagcggt caatgaattt gccagcccgc taacgggtac
     3001 agtttcttct gttaatgata aaatcggtga agatatcaat atcttgaatg attcagatga
     3061 aatgaatgcg tggatcttaa gtttcaaaga tgttgatcca aaagaatttg acgaattata
     3121 aaatcagaaa atcacttgaa aaaagttgac actgattctt ccagttgcta taatcataag
     3181 caatcctata tgaaacgaga aagctttgaa aagaagagta catgtcacgg acgtttgaag
     3241 agagccctgt ttgctgaaaa tgggtaacga aaatgagatg gaagatggtc ttagagcaga
     3301 gtaagcgagc gtaagcaaac ttactacggg agtgcccgtt acagcgcgac agtatacgga
     3361 aagaagctaa gtccaagtac tggaagaggc tgttattgcg aagtaacggt aaatcaaggt
     3421 ggtaacacgg aagttcaagc tttcgtcctt ttgcttaagg caagagggcg aaggcttttt
     3481 tgtttgaaaa attataagag aaagatgtga atggcatgtc attaatccaa ctaacgaatg
     3541 tcaaaaagac attcaatggg aaaaacggaa acatccaagc cgtcaatgac gtttcactaa
     3601 acgttgaaaa aggggatatt tatggaatcg tcggctattc tggcgcagga aaaagtacat
     3661 tagtccgtct cctgaatgga cttgagcttc ccacgagcgg cgaagtgatc gtaaacgatc
     3721 aagatattac aaaattaaaa aacaaagaat tacgtacatt ccgaaagaaa atcggtatga
     3781 tcttccagca tttcaacctg ttatggtcaa gaactgtact tgaaaatatc cagctgccgc
     3841 tggaattagc tggcgtacca aagagcaaac gtaaagaacg ggcagaagaa ctgttgcgtt
     3901 tagtcgggtt agaaggacgc ggacaagcat atccttcaca actttccggc ggacagaaac
     3961 aacgcgtcgg tatcgcacga gcattggcaa atgatcctga gatcctgctt tgtgatgagg
     4021 ctactagcgc attggacccg caaacaacag aagaagtatt agacttattg ttagcgatca
     4081 ataagaaatt gaatttgacc atcgtcttga tcacacatga aatgaatgtg atccgcaaaa
     4141 tctgcaacaa agtcgctgtg atggagttgg ggaaagtcgt agaagaagga gacgtattga
     4201 ctgtcttcag acaaccgaaa aaagaagtaa ccaaacgctt tgtacaacaa gacatcgaac
     4261 cagaagctga ttcgacagaa ctattagctg aactcatcaa agaaaatcca agcggccgtt
     4321 tggcaacttt gacctttagc gaaagaaatg ccaacgagcc agtcatttcc caagcgattc
     4381 gacgctataa catcgatgtc aacgttgtgt atggaaagat caaacaagtg aaagaaggtt
     4441 cctttggttc attgactgtc ttgatgactg gtgaaaccaa agaattagat caagcagaag
     4501 tgttcatcaa agaacaagga gtaggaattg aggtgatcca tcgtggataa aagcttttca
     4561 gaaacatatc tagattttaa acaaatcaac ccggaaacga tgaatcaggc tatccttgat
     4621 acactatata tgacattaat ctcaatggtc ttagtgacga tcatcggatt agttttggga
     4681 ttgatccttt actctattgg acgcaaaaac aaaccgtatg ctcgaatcat ttatggaatc
     4741 gtatcgatca tcagtaatat tttccggtca acgccattta tgatcctgat ggttttgatc
     4801 attccattta ctaaagcaat cgtaggcact atgctaggtg cgcaagcagc cattccagct
     4861 cttgttttat ccgcagcacc attttatgcc cgtctagtag agattgcttt tcgtgaagtc
     4921 agccctggtg tactggaagc agcagatgca atgggagcaa gttactggga aatcatctgg
     4981 aaggtcttga tacctgaaag tattcccgca ttgatttctg gattaacagt tacaacgatc
     5041 tcgatgatcg gatttactgc gatggccggc gcgatcggcg caggtggact tggaggtctt
     5101 gcttggcagg aaggctacca gcgtggtaat ctaacagtga cattcgttgc gactttaatt
     5161 attttactaa tcgtatttat cgttcaagga atcggtgatt tcttaactaa gaaaacagac
     5221 aaaagataat aggatatagg gggatgtagg atatgaaaaa gaaaattttt ggatttgcag
     5281 cagcgctttt gctgactgta ggattagcag catgcggtaa caacagcgac agcaaagatt
     5341 cagcaaataa agcagacgac acgacattga aagtcggcgc ttcaccgaca ccgcacgcag
     5401 agattttgga acatgtgaaa ccattattga aagatgaagg aatcgatctt gaaatcgtca
     5461 aattcgatga ctatgtatta ccgaaccaat ctttggaaga aggcgacatt gatgccaatt
     5521 acttccagca tattccttat ttgaacaaag aaatcaaaga aaaaggatat gactttgtca
     5581 atgccggtgc tgtccacatc gaaccaatgg gcttgtactc taaaaaagtc aaagatgttt
     5641 ccgaattaaa agatggtgca acaatcatta cttcaaattc tgaatcagac tggggacgca
     5701 tcatcacaat tctacaagat gctggcttag tcaaagtcaa agatggagta gatctggaaa
     5761 cagcaacatt cgatgatatt gaagaaaacc ctaagaactt gaagttcaac catacgatcg
     5821 acccatcact tttagcttct acgtaccaaa atgatgaagg tgacctagtt gcaatcaacg
     5881 caaacttcgc ttacggcgca gggttgaatc cattagaaga tgccgtattg cttgaaaagg
     5941 acaactctcc ttacgtgaat attattgcga ctcgtaaagg tgatgaagac agtgacaaaa
     6001 tcaaaaaact gattgaagtg cttcaccgca aagaagtcca agaatggatc acagacaaat
     6061 ggggcggctc tgttaaacca gtaaaggctg atgcaaaata aggtatggaa caaaaaagat
     6121 gagaaataag tctgtaatgg cttatttctc atctttttta tacctattct aatttagaaa
     6181 tcaaaattgt ttaataataa ataaaagata tttgatttta gtaaggaagg tcattgcgct
     6241 tcattctcaa ttagtctaaa atgagtgaga acgaaagaga atttgggggt ataatgcatg
     6301 tctgtcttag aaataaaaaa tctacatgtg tcgatcgagg aaaaagaaat ccttaaaggc
     6361 gtaaacctaa caatgaaaac cggagaaatc catgccatca tgggacctaa cggcacagga
     6421 aaatctacct tatctgccgc tatcatgggc aatccaaact atgaagtaac agaaggcgaa
     6481 attttgtttg acgggaaaaa tgttttagac ttggaagtag atgaacgtgc gcgacttggc
     6541 ttatttttag ctatgcaata tccaagtgag ataccaggta tcacgaatgc ggaattcatg
     6601 cgtgcagcga tcaatgcgaa acgtgatgaa aacgacaaaa tttctgttat ggcatttttg
     6661 aaaaagttag atgaaaaaat ggctttacta aatatgccag aagaaatggc tgaacgttat
     6721 ctgaacgaag gtttttctgg cggagaaaag aaacggaatg agatcctgca attgttgatg
     6781 ttagaaccta catttgcaat tttagacgaa atcgattcag gtctggatat cgatgctttg
     6841 aaagtcgttg ctaaaggagt aaatgaaatg cgcggagaca atttcggtgc attgatcatc
     6901 acccactatc aacgattatt gaactatatc acacctgatg tcgttcacat catgatggaa
     6961 ggccgagtag tcatgacagg gaatgctgac ctagctaaac gtctggaagc agaaggctac
     7021 gctggtatca gtaaagaatt aggaatcgac tataaagaag aagaagcgta agggggagca
     7081 agaatgaaag aaaaaaactg gctcgattat cttgacgcgg taaatgattt ttctttaagt
     7141 aaaggcgaac ctgattggat gagaaccttc cgtcaagacg cactagcgaa agcagatgaa
     7201 ctgccattgc cgcatatcga tcgggtgaaa ttccatcgct ggtcgttatt tgatgtgaaa
     7261 gaaacacaaa ccatcagtga aacaggcacg attccggcat ttgatgcaat gaaagacaat
     7321 cctgtgcttg tccaacaagg ttcatggacg atttttgaac aactgcctgt ggaattagca
     7381 gaaaaaggag tcatttttac cgacttgttc actgcaatga tcgaatatcc agaactagtc
     7441 caagaatatt atatgaaaaa agcagtaaac atgaacgaag accaattgac tgcactccat
     7501 gtagctttta tgaacagcgg tattttcctg tatgtgccta aaaatgtcgt gatcgatgaa
     7561 ccactcgagt ccttattcat tcaagatggc gcatccgatg agcacttctt caagcatgtg
     7621 ttgatcgtgg cagatgaaca tagtgaattt tcttatctgg aacgtttcca gacaaccaaa
     7681 gaacaagtag caaaatcatc aggtaatatc atcgtggaag tcatcgctaa agctggttca
     7741 aaaatcaaat attcagccgt agatcaatta ggagaaaata ttacttctta tatgaatcgc
     7801 cgtgggcaca ttttacgaga tgcttctgtc gactgggcaa ttggtgtcat gaatgacggt
     7861 catgtgatcg ctgactttga ctcagattta gcaggagaag gtgcacacgc agaagtcaaa
     7921 atcgtggcaa tcagctcggg cagacaaatc caaggaatcg atacacgagt aaccaataaa
     7981 gcccctcata cgatcgggca tattttgcaa cacggagtga tccgagaaaa aggaacattg
     8041 acctttaatg gtatcggcca catcttgaaa ggggcaaaag gtgcagatgc ccaacaagaa
     8101 agtcgtgtct tgatgctttc tgataaagca cgtggtgatg cgaatcctat tcttttgatc
     8161 gatgaaaacg aagtaactgc tggacatgct gccagtgtag gacgagttga tccagaagaa
     8221 atgtactatc tgatgagtag aggattgcat aaagaagaag cagagcgctt agtgattcga
     8281 ggattcctcg gttctgtctt aacagcaatc cctgtagaac aagttcggaa agaattagtc
     8341 gaagtaatcg aagggaagtt gaatggatga tcgatgctaa aaagctacgc aatgattttc
     8401 ctatcttatt tcaagacgtc aatgatgaac ctttagttta tctggataat gcagcgacga
     8461 cccaaaagcc aaaacaagta ctccaggcat tagcgtacta ttacgaacac gataacgcca
     8521 atgttcatcg cggtgtccat acgttagcag aacgcgcgac ccatgattat gaacaagcaa
     8581 gagaaaaagt acgggcgttc atccatgcaa aagaaacagc agaagtgctg tttactcgtg
     8641 gtacgacgac aagcttgaat tgggtagcca gaaccttcgg cgaccgctat atcaaagcag
     8701 gagacgagat agtgctctct tacatggaac atcattctaa tgttatcccg tggcagcagt
     8761 tggcaaaacg aacaggtgct gtcttgaaat atatcgaact aacagaagaa ggcttcctgg
     8821 atatggagga tgcagcgaaa aagattacgg acaaaaccaa aatcgtctcc attgctcatg
     8881 tatccaatgt cttaggtgtc ttcaatcctg ttcaagaatt agctgaactt gtccatgaaa
     8941 acggcggggt catcgtagta gatggtgctc aatctgcccc gcacatggcc attgatgtcc
     9001 aagcactaga ctgtgatttc tttgctttca gcggacacaa aatgtgcgga ccaacaggta
     9061 ttggtgtttt atatggtaaa cgaaaatggt tagaagaaat ggaacctgtt gaatttggtg
     9121 gcgaaatgat cgattttgtc gaattatacg acagtacatg gaaagaacta ccttggaaat
     9181 tcgaagcagg gacaccaaat atcgccggcg ctatcgcatt aggccatgca atcgattatt
     9241 tgcaaaaact cggtatggac aatatccatc gatatgaaga agaactagcc gcttatgtgt
     9301 tgccaaaatt acaagcaatt gatggattga ctgtttatgg tccacaagac ccgaattttc
     9361 ggacaggagt cgttgcattc aatttggatg ggttacatcc ccatgatgtc gcgacagctt
     9421 tagacatgga aggggtagcc gtcagagcag gtcatcattg tgcgcaaccg ctattgaagt
     9481 accttgaagt atcagcgact gctcgcgcga gcttttattt ctacaacaca acgcaagacg
     9541 ccgaccgttt agttgaagca atcatggcaa caaaggagtt tttccaacat gggactatct
     9601 aaattagaca atttataccg tcaagtgatc ttggatcatt cttcccatcc gcatcatcgt
     9661 ggaagcttga ctgactcgac aagcaagatc gaattgaaca accccacatg tggagatgtg
     9721 atcgaattgc aagtcgatgt aaaagacgga atcatcaaaa atatcgcctt tgacggcagt
     9781 ggctgctcca tcagtacggc aagtgcctcg atgatgacag acgccgtcat cggaaaatca
     9841 atagaagaag cagaacattt aactgtagac tttctacagc ttgtccaagg aaaagaagtc
     9901 aaagatcccg accaattagg cgatgctgct atgcttggtg gtgtagctaa atttcccgct
     9961 cggatcaaat gtgcgacact tgcctggaaa gcactaggaa aagcaatgga aaacgatggt
    10021 accgcagctt tccatcatta ccacaaggaa gaggagtgag aacatgggcg taccagaatt
    10081 agaagaatat aaattcgggt ttcacgatga tgtcgaaccg gttttcagta caggagaagg
    10141 cttgacagaa gaagtgatcc gtgagatgtc aagaatcaaa ggcgaaccag aatggatgct
    10201 tgaattccgt ctgaaatctc tagaaacatt taacaaaatg ccaatgcaaa catggggccc
    10261 cgacctttca gacatcgatt tcgatgccat caaatattac caaaaaccaa gtgacaaacc
    10321 cgctcgtgac tgggaagacg taccagaaaa aatcaaagat acatttgaaa agattggtat
    10381 accagaagct gaacgagctt atctagccgg tgcttccgca caatatgaat cagaagtcgt
    10441 gtaccataat atgaaagatg aattcgaaaa gttaggaatc gtctttactg acactgactc
    10501 tgctttgaaa gaataccctg aattattcaa agagtacttt tcaaaacttg taccgccaac
    10561 cgacaacaaa ttagcagcgc tgaattcagc cgtatggtca ggtgggacct ttatttacgt
    10621 gccaaaaggc gtcaaagtcg atgttccatt acaaacgtac ttccggatca atgcggaaaa
    10681 cacggggcaa ttcgaacgta cgctgatcat tgttgacgaa ggagcaagca tccactatgt
    10741 cgaaggttgt acggcaccta cttattcaag taacagcctg catgccgcca tcgtagaaat
    10801 ctttacaaga aaagatgcgt attgccgtta cacaacgatc caaaactggt cagacaatgt
    10861 gtacaactta gtaaccaaac gagcaaaagc atacgaaggc gcaaccgttg aatggatcga
    10921 cggaaaccta ggtgcaaaaa caacgatgaa atacccaagt gtttacttag atggaaaagg
    10981 tgcacgaggc accatgctct ccatcgcctt tgcaggagca aaccaaatcc aagatacggg
    11041 tgcaaaaatg atccacaatg caccaaatac atctagctct atcgtctcta aatcaatttc
    11101 aaaagacggc ggagaagtaa actatcgagg acaagtaacc tttgccaaaa acagtgcggg
    11161 ttcgatctct catatcgaat gtgacacgat catcatggac gaatggtcga aatccgatac
    11221 gatcccattc aatgagatcc acaacagcca agtctcttta gaacacgaag ccaaagtctc
    11281 aaaaatctca gaagaacaac tctactatct gatgagccga ggcctgaccg aatctgaagc
    11341 aactgaaatg atcgtcatgg gctttgttga accattcacc aaagaacttc caatggaata
    11401 tgcagtcgaa ctcaatcgat tgatcagtta tgagatggaa ggcagtgttg ggtaaacctg
    11461 ttataggagc tagaaccctt gatata
//
LOCUS       DBKOIJ010000087        11448 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000087 DBKOIJ010000000
VERSION     DBKOIJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.089"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(143..628)
                     /locus_tag="ACV5NJ_001794"
     CDS             complement(143..628)
                     /locus_tag="ACV5NJ_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HHY6485877.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    718..952
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1108..1749
                     /locus_tag="ACV5NJ_001795"
     CDS             1108..1749
                     /locus_tag="ACV5NJ_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHY6485878.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1768..2403
                     /locus_tag="ACV5NJ_001796"
     CDS             1768..2403
                     /locus_tag="ACV5NJ_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HHY6485879.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2421..3236
                     /locus_tag="ACV5NJ_001797"
     CDS             2421..3236
                     /locus_tag="ACV5NJ_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HHY6485880.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3555..4991
                     /locus_tag="ACV5NJ_001798"
     CDS             3555..4991
                     /locus_tag="ACV5NJ_001798"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HHY6485881.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5180..7627
                     /locus_tag="ACV5NJ_001799"
     CDS             5180..7627
                     /locus_tag="ACV5NJ_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HHY6485882.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7857..8924
                     /gene="rlmN"
                     /locus_tag="ACV5NJ_001800"
     CDS             7857..8924
                     /gene="rlmN"
                     /locus_tag="ACV5NJ_001800"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HHY6485883.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8914..9120
                     /locus_tag="ACV5NJ_001801"
     CDS             8914..9120
                     /locus_tag="ACV5NJ_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485884.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9117..10220)
                     /locus_tag="ACV5NJ_001802"
     CDS             complement(9117..10220)
                     /locus_tag="ACV5NJ_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HHY6485885.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10773..11321
                     /gene="aac(6')"
                     /locus_tag="ACV5NJ_001803"
     CDS             10773..11321
                     /gene="aac(6')"
                     /locus_tag="ACV5NJ_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HHY6485886.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3799 a         1940 c         2472 g         3237 t
ORIGIN      
        1 actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat
       61 tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg
      121 aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa
      181 caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat
      241 gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct
      301 ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag
      361 gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt
      421 ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg
      481 atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg
      541 acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat
      601 aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat
      661 cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa
      721 atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa
      781 gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga
      841 aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt
      901 tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga
      961 tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg
     1021 aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat
     1081 ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta
     1141 ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca
     1201 ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt
     1261 caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt
     1321 tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt
     1381 atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct
     1441 caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa
     1501 atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac
     1561 ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg
     1621 aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat
     1681 cttacattga ctggtattct aacaatcata atgaaacaag ttgaaggaaa attaaattat
     1741 taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt
     1801 ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct
     1861 atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa
     1921 agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa
     1981 atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat
     2041 atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac
     2101 accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat
     2161 ccttatcagc tttctggcgg acaaaaacag cgggtagcta ttgccagagc attggctatg
     2221 aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag
     2281 caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc
     2341 catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat
     2401 taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc
     2461 ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca
     2521 acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag
     2581 ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat
     2641 atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca
     2701 atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag
     2761 tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag
     2821 gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg
     2881 gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg
     2941 ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg
     3001 gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag
     3061 acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt
     3121 cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg
     3181 aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa
     3241 taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct
     3301 gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt
     3361 caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
     3421 caaaattttt cttactactc gaaactaagc gactttttca caacctcctt tacataattt
     3481 tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg
     3541 aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg
     3601 gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat
     3661 cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt
     3721 tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc
     3781 tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag
     3841 tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata
     3901 ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata
     3961 atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg
     4021 aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt
     4081 cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg
     4141 aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag
     4201 aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta
     4261 atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg
     4321 atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat
     4381 gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc
     4441 ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg
     4501 ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa
     4561 tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc
     4621 cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga
     4681 ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg
     4741 actatcgaat caattactta caaagccacg tcaaagccat caaagaagca atttcggatg
     4801 gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg
     4861 gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt
     4921 tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag
     4981 agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt
     5041 cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa
     5101 gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg
     5161 atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac
     5221 gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt
     5281 tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat
     5341 tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc
     5401 cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg
     5461 ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt
     5521 tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt tgttagccag
     5581 tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct
     5641 ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt
     5701 actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat
     5761 ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg
     5821 aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg
     5881 cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt
     5941 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
     6001 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
     6061 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
     6121 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
     6181 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
     6241 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
     6301 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
     6361 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
     6421 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
     6481 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
     6541 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
     6601 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
     6661 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
     6721 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
     6781 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
     6841 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
     6901 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     6961 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     7021 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     7081 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     7141 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     7201 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     7261 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     7321 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     7381 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
     7441 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
     7501 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
     7561 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
     7621 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
     7681 gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg
     7741 tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa
     7801 ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg
     7861 aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg
     7921 gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta
     7981 atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta
     8041 tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt
     8101 tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct
     8161 tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg
     8221 ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg
     8281 tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg
     8341 ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg
     8401 atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac
     8461 acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg
     8521 cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga
     8581 aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat
     8641 atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat
     8701 taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac
     8761 atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga
     8821 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
     8881 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
     8941 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
     9001 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
     9061 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
     9121 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
     9181 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
     9241 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
     9301 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
     9361 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
     9421 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
     9481 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
     9541 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
     9601 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
     9661 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
     9721 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
     9781 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
     9841 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
     9901 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
     9961 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
    10021 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
    10081 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
    10141 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
    10201 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
    10261 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
    10321 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
    10381 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
    10441 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
    10501 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
    10561 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
    10621 aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag
    10681 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
    10741 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
    10801 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
    10861 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
    10921 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
    10981 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
    11041 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
    11101 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
    11161 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
    11221 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
    11281 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag
    11341 aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg aatttttgtg
    11401 aaattccaac ttatttccga agaagctact tttggaacac cgtgtacc
//
LOCUS       DBKOIJ010000088        11198 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000088 DBKOIJ010000000
VERSION     DBKOIJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.090"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACV5NJ_001804"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACV5NJ_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HHY6485887.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACV5NJ_001805"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACV5NJ_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HHY6485888.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACV5NJ_001806"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACV5NJ_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HHY6485889.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACV5NJ_001807"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACV5NJ_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HHY6485890.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="hpf"
                     /locus_tag="ACV5NJ_001808"
     CDS             complement(5664..6215)
                     /gene="hpf"
                     /locus_tag="ACV5NJ_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HHY6485891.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACV5NJ_001809"
     CDS             complement(6343..7062)
                     /locus_tag="ACV5NJ_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HHY6485892.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACV5NJ_001810"
     CDS             complement(7059..8372)
                     /locus_tag="ACV5NJ_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HHY6485893.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACV5NJ_001811"
     CDS             complement(8453..8773)
                     /locus_tag="ACV5NJ_001811"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6485894.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACV5NJ_001812"
     CDS             complement(8881..9168)
                     /locus_tag="ACV5NJ_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7662 domain-containing protein"
                     /protein_id="HHY6485895.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACV5NJ_001813"
     CDS             9295..9939
                     /locus_tag="ACV5NJ_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HHY6485896.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACV5NJ_001814"
     CDS             complement(9998..10705)
                     /locus_tag="ACV5NJ_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6485897.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACV5NJ_001815"
     CDS             complement(10925..11095)
                     /locus_tag="ACV5NJ_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485898.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DBKOIJ010000089        11000 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000089 DBKOIJ010000000
VERSION     DBKOIJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.091"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..436
                     /locus_tag="ACV5NJ_001816"
     CDS             <1..436
                     /locus_tag="ACV5NJ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HHY6485899.1"
                     /translation="IYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFN
                     EFMTEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIE
                     TGLVRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
     gene            436..1011
                     /locus_tag="ACV5NJ_001817"
     CDS             436..1011
                     /locus_tag="ACV5NJ_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HHY6485900.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            1036..4707
                     /gene="brxC"
                     /locus_tag="ACV5NJ_001818"
     CDS             1036..4707
                     /gene="brxC"
                     /locus_tag="ACV5NJ_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HHY6485901.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            4771..8403
                     /gene="pglX"
                     /locus_tag="ACV5NJ_001819"
     CDS             4771..8403
                     /gene="pglX"
                     /locus_tag="ACV5NJ_001819"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HHY6485902.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            8519..>11000
                     /gene="pglZ"
                     /locus_tag="ACV5NJ_001820"
     CDS             8519..>11000
                     /gene="pglZ"
                     /locus_tag="ACV5NJ_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HHY6485903.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFI"
BASE COUNT         4132 a         1493 c         2084 g         3291 t
ORIGIN      
        1 aatctatcag aacgtgaaaa ggagagttag tcaactggat gaagctggat tgcaactttt
       61 caatcagtta aatatagata atcaaaagtt agtgaatttg atttccatcc ttatgattga
      121 tgatcttttt aatgaattta tgactgaggt atatgctgaa aatctacgta aaggaaattt
      181 gcaattaaca ccagttgatt accgttcttt ttttacagag aagcggagaa gttcagaaga
      241 tatttcaaaa tggaaagatt atacgattaa acggcttatt gccgcttatc aaacgaatct
      301 aattgaaaca ggattagtca gaggggaaaa agaatatgga cgaatgactc cgatattatt
      361 agataaacga ttgattcaat ggttagttga caagaatcgt aaagatattg tactagcact
      421 gggaggaatt ttataatgtc attatcgatt caagagcatt tggaaaagtt gaatgaagta
      481 attcaaaaag aagatttttt attgaatcgt ggtttaggta atgaagtagg ctattatatc
      541 tttgactacg atccgaaaga cgagttaatt gtaagagatt atattaatga tatatctgga
      601 acatctgttg gtgataatta tgaagtgaaa gtttataatc tctatgtgat tatgttagag
      661 tatctagaaa aacgcggttt cactgaaaaa gcgattaaga tggaagaacg acgtggttta
      721 caaaaattag tggaaagcat tggtcgttta ttgaaaatgg atttacctga ggaagaaaat
      781 gtattttttg aatatgtgga tgcccataca ccagaaaaat cagttgtgtt cctgacagga
      841 attggcgaat tatatccatt aattagagcg cactctattt taaataaaat gcatcaggta
      901 tttgatcgcg taccagttgt gatgatgtat ccaggggaat atgatgaatt aagtttgaca
      961 attcttaata ccaataaaga tgataattat tatcgagcat ttagaattta gttgggatgg
     1021 aaggtaggag attaaatgct aattaaagat atttttgcaa aaccaattga ccgaaatatt
     1081 caaggagtta ttaaggtcgg tcaagcaaag gatgaaaatg ttcaacaaga attagaagaa
     1141 tacgtggtta cgaaagaact acaaaaacac tttaaaacaa tttttgatgc ctaccaacgg
     1201 agtatcaata caccaacaga taaaatgggt gtttggattc aggggttctt tggttcaggt
     1261 aaatctcatt tcttaaaaat tatttcttac ttgctcgcaa atcgagaaat aaatgacaag
     1321 aaagccattg attacttcaa agaagatggg aaaatcaaag atccactgac tttggcaacg
     1381 atggaacggt gctcacaaat aaatacagat gttattctgt ttaatattga ttcaaaaagt
     1441 gatgcaggtg gtaaaaaaga caaaaacgga atcgtgaatg tttttttaaa ggtatttaac
     1501 gaaatgcaag gattaagcac gattccacat ctagcagatt ttgaacgtcg gttaattaaa
     1561 gaagaaaagt ttgatgcatt caaagctgct tttgaagaga ttaatggggc aaaatggcaa
     1621 gaagagcgcc atgagtttga ttggatccaa gatgatatca aagaagcggc acttaaaatc
     1681 gattttatga gtgaagccgc tattgataat tttttagaaa aagcatcagg aaattattta
     1741 ataagcattg atgactttgc aaatctagtt aaaagctatc ttgattccaa agaagcagac
     1801 catcatattg cctttctagt cgatgaggtc ggacaatata tcggcggtaa tcgtgagttg
     1861 actttgcagt tacaaaccgt tgtggaagat ttgggtcgaa tttgtgaagg taaggcttgg
     1921 gtattagtga caagtcagga agcgattgat cacgtaacaa actttgataa caattctgat
     1981 aatgattttt ctaagatcaa ggatcgtttt gccacaactg tgagtttatc ttcagcaaat
     2041 gccgatgaag taattaaaga acgtattttg aagaaaaaag agtttgccaa tgattcttta
     2101 gccttacaat ttgaaactgt tcaaacagaa ttgaaaaata aattactctt tgttgatagt
     2161 cctgaattaa agctcttcca agatgctgaa aattacgcag aagtttatcc atttattccc
     2221 tatcaattca atttattggg gcaaattcta accgagattc gtaatcacag ctatcaagga
     2281 tcaaacttat cgtctggtga acgttcatta ctagcaatgt ttaaagaagc cgctgaacga
     2341 aataaggaaa atgatacaac cagtttggtt tctttagatc agttttatta ctcgttagaa
     2401 cgttggattg atacagcaat tagccgagtg attacacaag ctacaaataa ttctcatatt
     2461 caaagaccaa ctgatataga taactttaac gtaaatgtct tacgaatttt attcatgatt
     2521 aaatatgttg atcaagccat caaaccaaat gttgaaaata ttacgagctt gatgataaga
     2581 aatatcaacg aagaccgcct ggaaactaaa aaacgagtag aagaagcatt ggacctttta
     2641 attaaggaaa actttgtgcg gaagaactta gatagttata ttttccaaac tgatgaagaa
     2701 caggaaatta ctcgtgagat taaaaatcaa aatattacag atagtgattt gatcgtagaa
     2761 ctatctaaac gaatttttga cggcgttcta gaccgaaata gttttgccta tgaaaaatta
     2821 ggcatgtcag gtggaaataa atttaaaaac cggtatacgt ttacttatgc acaagtgtta
     2881 gatcaacgtc catacaagca agctggaaat gctgaaatga cagttaattt tatttcacca
     2941 tactctgatt tagcaggtca agaggcagaa cttgcgctta agagttcccg taacttatta
     3001 ttcgtagaat tgccatcaga taaagaattt aaagatgata tagcaatgta tgctagaatg
     3061 aatctttatt taacaacgac ttattcgagt gtagtaaaga actttgacgc gattcgttcg
     3121 gaaaaatctg gtgaattaca ggaactaagt gcaagtatca acgatcaatt aaatgaactg
     3181 ttgaaatctg caaaattttt ctataacggt gcagaaattc aactgacagg ctccgacttt
     3241 aaagcaaaat tccaatcagc acttcagaaa atggctgaag aagtatacca taaattaaac
     3301 caaattgatg cgcctaaaga aaaagaagat atcttatatt tagtgaaaga aagcaaaaat
     3361 gaaattattg aagatccatc aaatgagcaa gcagttcagg aaattttcaa ttttatcaat
     3421 gatcaaaaga gaaatcacct aacgattacg atgaaaacaa ttcgtgatcg ttttgaaaca
     3481 aaacaaccgt atggattcac agaacttgat gtggaatggt gcatcgctaa acttttcatg
     3541 tctggtaaaa ttgatttact ttataacaat gtacaaattt cacggatgga tgatccaaat
     3601 agtattgtaa tgattattca aaaacgacga gaaagtgaaa agataaaaat tgaaccaagc
     3661 gcggtgattt cgccaaaagc aaaaaaattg gttgacgaaa tcggtagaga actgtttcaa
     3721 agtcgtaata ttgttgatga aaataacaat gaacatacaa tggaaaattt caagaaatat
     3781 ttaaaaggtc aattagacaa tttacgctct tattttgatt tacaaaacac ttatacctac
     3841 cctagtgctt atgaatatga gcaaataaaa aaatatcttg aagtaattgt tcaaacgtca
     3901 gatgttgaag acttttttta tacaatcaaa aaatatcgcg atgaattatt cgactgggct
     3961 gaaacatatg ataaaattaa aaatttccac aagcaaaata gtactcagaa aaaaatctgg
     4021 gagaatgcac aagataaaat tagattaatc gaaaaaagtc ggcgtcaaac ttctagccaa
     4081 gaaattgatc aattgattca tgagatgaaa accattttga aaaaggctga tccttattca
     4141 gatatgatga aactaaatga gttgattcaa gaatttattg aacaatataa tcaactgtta
     4201 gagttggaaa gtcaggaaat attaaataaa ttaacggaag aaaaacaagc aacatttaaa
     4261 ttattagaag gaaaagattt taaggatcaa tttgtaccaa tagttgacca agaatttcaa
     4321 gaactgaaag caaaaattga acaagcgaat actatagatg atcttgtttt cttatcatta
     4381 aacgtagcag aacagtattt gatctttaat caacgtttcg ataatgaaga aaaccgcaga
     4441 cgtcaagaag aacaagcacg taaacaaatt gaagcagcaa acttgattcg tgaaactgaa
     4501 aaagatgaaa tagactctgc agcaaaagct gaagaaatag cgatagtaaa acctactatt
     4561 caacagaaaa cagtaccaat gagaacactt aacgttcatg ttaaaactat taagtcagca
     4621 gcagatattg atgagttaac agctgaaatt aatcaaaaac tgaaggaaca acttcaagaa
     4681 aatacagaaa tcacgattca attctagtga aaagacaggc ataaaaaggt gcctgtcttt
     4741 tcaaaggtta catatagaag ggaaagaaaa atggataaga aagcgataaa gaatttcgcg
     4801 gtcaatgccc gtagaaaatt aattgatgaa gtaatgacta aagcaaaacg tttaggaatt
     4861 gatgaatcaa aaattgaaga tgtgaagaaa attgatagca atttacaaga aatcgaaaaa
     4921 tcaggaataa gaattcaagg aaaagaaata gcgcaaagaa acaaattgat tgcagaacta
     4981 aaacaacgtg cttctgtcac ttcttattca gaagcttttc aagagttaat cgaagaggtt
     5041 gcttatacgt ggtttaaccg attgattgcc attcggttta tggaggtaaa tgattattta
     5101 cctgacacgt atcgtgtatt atctagtgaa ataagtggga aaattgagcc agatattatt
     5161 acagatattt atgatgccga tatctttgaa gaattatcta gtaatgaaca gcaacaagtc
     5221 agaacttgga aaacagataa ttcagcagaa gcaatggaca agctgtatca attgattttt
     5281 ataaaaatct gtaatcaatt gaatgattac ttacctgagc tatttgaaga agtagaagat
     5341 tatacagaac ttttgtttac agcttcttat attaaaaaag atggtgtaat cgctgatctt
     5401 ttaagtattc cagaggatga tttctatgtt caagctggtg gacaggtaga gattattggg
     5461 tggttgtatc agtactataa tagtgagcca cataatcaag tggtgaatat ttataaaggg
     5521 attgtcaaaa aacaagatat tccggcagca actcaactat ttaccacaga ttgggtcgtt
     5581 cgttatatgg ttgacaatag tttaggaaag tattggttag atcattatcc caatagtgaa
     5641 ttaagaaata agttgaaata cttaattcca gataaatatg cttttgaaca agcaacatct
     5701 gtaagtaatt taaaaccaga agaactaaaa attatcgata acgcaatggg gtctgggcat
     5761 attttagttt acgccttcga tgtattgatg gaaatttatc ttacgttagg atattctgag
     5821 cgtgatgctg cacgtgcaat cgttgaatac aatttatatg gtcttgaaat cgataaaaga
     5881 gcgtatcaat tgtcttattt tgcaattatg atgaaagggc gtcaatacga tcgtcttttt
     5941 ttgaaaaaaa gtctatcgcc gaatttaaga gagtttattg attctcctaa agtgacagac
     6001 gagtatcaag agcgtattag tgaattgaac aatgataagt atagtgaacg ctggaatgaa
     6061 tacaaagagt taatttttga tcaatttgaa aatgggaaag aacttggatc aatcattaag
     6121 ttagtcgata aaaataatca ggcaataact aaagaatact taggaaaagt aagagagtat
     6181 atatcaaaat tcaacgaatt tacgaatatg gatattcttt atgggatgaa ggaaatacaa
     6241 gaaaaatact tgcacattct tgacgtggct gaaatgctag ttgataaata tgtagcggta
     6301 gtcactaatc cgccttatct taataaaatg gataagaaat taaaaaaata tgtaaatgat
     6361 aactattctg atgtcaaaaa agatctattt tctgtgttta ttaaattgaa tagccaaatg
     6421 ctgattgata atggatatgc ttcctttatg acgccatttg tttggatgtt catttctagc
     6481 tatgaaaagt tacgctcatt tttaattaat gaaagaaaca tttctagttt gattcaaatg
     6541 gagtattcag cttttgaaga agcaaccgtt ccaatttgta cgtttacgat aaaaaattca
     6601 aatgaacaag atggaagcta tataaaatta tcagatttta aaggtggaat ggaagttcaa
     6661 aaaattaaaa cgttagaagc aattcatcag catggtgaat gcgattattt ttatagtact
     6721 aatcaagaca acttcaaaaa aatcccagga atgccgattg cttactgggc aagcgataac
     6781 gtaatcactg attttgctca aggaaaatta actaacgatg ttgcagatgc aaaacaaggg
     6841 ttggcaaccg gaaataacaa taaatttttg cggtattggt ttgaagtaga atttaacaaa
     6901 ataaagtttg atgctaaaac attagaggac gctaaaaagt ctagaaaaaa atggtttcct
     6961 acaactaaag gtggtcgttt tagaaaatgg tatggaaaca atgaatacgt ggttaactgg
     7021 gagaatgatg gagatgaaat aaaaaattat gttaatgaaa atgggaagtt aaaatctaga
     7081 ccacaaaatc ttaattacta ttttaaaaag ggaattactt ggtcgacact ttcatctgga
     7141 caagtatctt ttagattgca gaatcaggca atttttgaaa ctaaaggatc tgttctgttc
     7201 tcaaaggata taactgactt tgaagaaata tttggctatg taaactctaa attagttaat
     7261 cactttttaa cttttatttc tcccaccttg gattaccatg aaggaccaat gggaaaacta
     7321 ccttttctta aagttacatc tgagagaaaa ttaaaaaaaa taagagaaaa agtggatgaa
     7381 aatatcgata tagctagaaa agactataat gctcttgaaa cgtcatacga gtttagaaga
     7441 aacattttgt taagttatat gtttaaaaaa gatctctcag aggcatttaa ctgtctaagt
     7501 aggcagttgg ttaatcaatt taatactcta aaatcgaatg aagaggaact gaacgacata
     7561 ttcattaatc attattctct tgaaaaagaa ttaacaaaag aggttcaaga tgaatatatc
     7621 tctgcaaggt tacctgataa ggcgagggaa attaaggatt ttatttctta ttcaataggt
     7681 tgtatcttcg gtcgttactc cctagacgaa gatggtctca tcttcgctgg cggcgaatgg
     7741 gatccttccc gctatcaaaa atttaaacct gatactgata atgttttact catcaccgat
     7801 gatgagtatt tcaaggatag ccaaattgac attgtcaatc gtttcattga tttcgttgag
     7861 attacttttg gtaaagaaac ccttgaagaa aatctccagt ttatcgctga cactttaggt
     7921 ggcaaaggga caagtcgaga aattattcgt cgttacttcg tacaagattt ttacaaagac
     7981 catttaaaaa catactccaa tcgaccaatt tattggcaat tggactcagg aaaacaaaat
     8041 ggctttaaag cgttaatgta cttgcatcgc tatacaccag atcaattagg aaaagtacgt
     8101 actgaatacc tacacgaact acaaaaagcg tatgacaatc gaatcgaatt gcgtcagcaa
     8161 caaatttcaa caagtgataa cccaaaagaa agaaatgcaa tgacaaaaga aattgtaaag
     8221 attcagaagc aattaaaaga agttcgtgag tatgacgaga agttgggtca cttagcatta
     8281 aaacgaattg aattagactt agatgatggt gtaattgtca attatgataa gttgcagcgt
     8341 gatcctaaaa ccggcgagaa attccaaatt tttgcaaaag gggtcaaaga accaaaaaaa
     8401 tagttatcta agaaagttgt tttctaacaa ctttcttttt tattcttaaa ttgcgtgcga
     8461 aattatccaa attatgtcaa tataaggggt atagaaagag aacgattagg tgagattgat
     8521 gacagatata aatacgcaac aaattgaaga aaaattaaat caacgttttc ctaattatgg
     8581 atgtaggaaa atcatttttt ggtttgattc aaatcaggat tttcttgaag aaattgactc
     8641 attggtcatt caaaatgcaa aaatctatcg attagaaaag gacgcacaat ttaaaactaa
     8701 gcgtttattg gaatatgaag atcccgaaac taactattta atctatgcgc catttcctcg
     8761 ttgggatgat aaagatgaag ataatcattt gttgagtgta ttgaattatt cagaggaatt
     8821 tagtgcagac cgcttagcga ttatcatgaa tgaattggct attccactta gatttcatca
     8881 gacagttaaa cagtatcgta atttctttaa tgcaaaggat cgcatgagaa gttttggtga
     8941 atttagtacg catatgacaa ttaataaatc agaaacgatt gaattaatga taatggctgt
     9001 tttgttgaaa tcgaaaagta ttcgattaaa tgatttactg cgtgtagttc taaaaagctt
     9061 tacagaaagt agggagaatc ctttaaggga acttgataag tatcagttaa cagctaaatt
     9121 ttgggaattt attcaaatga aatttggctt tacggatagc cagccaacat tggaaaagct
     9181 gacgatgtgt ctttatttga actattttta ttatcaaatt gatcaagaga tgccgaagaa
     9241 gttcaaagga tttgaaatac tacatttgaa agcaaacatt attactttca tggatcagtt
     9301 tatgaatgat acacgttaca tggaaagctt tgaacagcta tctaaaaaag tatatcaggc
     9361 tattgatggt gatggattat tagacagtat agaaattgat gatctaattg aagcagacgt
     9421 attttctgag attcatgcaa aaatattaat gttttatgca gaacgattaa aggcttctga
     9481 cactacaacg aaaattgcag gacaaacaat ttttgatagt ttaaagcagc gcgaaaaaat
     9541 gcatttcgca tcggattata tttatgagta tcaattattg aaacatgctt attatcttgt
     9601 ggataaaaac agattgtgct tgccgatgaa catggataat ttgctaactc aatatgagaa
     9661 ggaaatgtat tgggttgata cgcattatcg gaaatttatt tggaatagtg atcatataga
     9721 agaaaagcaa aaatatggcc aattgtatcg attagttgaa gcaaattatt cgaagtttct
     9781 tgatcaatca ggacaaattt ggaatcaaac ttttgaattt aagaatcgtc catccattcg
     9841 aaaattttat aatcaagctg taatgaaacg ctctgttaaa actgtagtta ttatttcaga
     9901 tgcttttcga tatgagttag ggatgaattt agaacgtaag ttagttagag aaaagaagta
     9961 ttcaacaaac atggctagct ttctttctgt attaccttca gttactgaat tcggtaaagc
    10021 cgccttatta ccaaacaatc aactaactta caatgaagga acagaagtac tgattgatgg
    10081 gaaaaaaacg aatggtttat ccaatcgaaa tgcgattctc cagcaacgag atgcaaatgc
    10141 tgtagcttta aattatgatg atgtggttaa gatgaataag gctgaggtac gagaagtatt
    10201 taatggcaaa tcagtcattt atatttatca cgatcaagtg gatagaacag gtgatcactc
    10261 gaatgaaaat cgagtttttg aggcctgtga tttagcgata agcgaaattt cccgacttat
    10321 taatcgcttg cataatgatg gcagtatcta tcgttttata gttacggctg atcatggatt
    10381 tatttataaa agaagccacg ttgaagaagt agataagatt gaaaatccat ctagaaatga
    10441 aaaagatcgt gtagaacgtc gttttattct tagtgaagaa gaatacgaaa taattggtac
    10501 gaaaaattac tctttgggag attctttagg aaatgatgac actagaaaca tttgtgtccc
    10561 gattacttca agtattttta agaaagctgg cgggggacaa agctacgttc atggagggtc
    10621 atctattcaa gaaatgctag tccctgttct agaaattacg gtagccaaag gtagcaatgc
    10681 aaaggaacca gtagcaattg aattaatgac tagcaaccgc agaattacag gattatcaac
    10741 cacgttagaa ttctatcaaa aaaatgcaat tagtgatgtt tatgaccccg catctttctg
    10801 gctttatttt gaagatgaat atggtgaacg tatctcaaat gaagaaatct atgttgcgga
    10861 ttcaaaaaca gaagatgcaa atcagcggtt tactaaattt gtttttgaat ttgtaaatcg
    10921 tcgatataca gttgaagata cttattattt agtgataaaa aatcaaaaaa atgatttgac
    10981 cgagcgaata aaattcatta
//
LOCUS       DBKOIJ010000090        10876 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000090 DBKOIJ010000000
VERSION     DBKOIJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.092"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(117..494)
                     /locus_tag="ACV5NJ_001821"
     CDS             complement(117..494)
                     /locus_tag="ACV5NJ_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485904.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(794..1675)
                     /gene="galU"
                     /locus_tag="ACV5NJ_001822"
     CDS             complement(794..1675)
                     /gene="galU"
                     /locus_tag="ACV5NJ_001822"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HHY6485905.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1718..2737)
                     /locus_tag="ACV5NJ_001823"
     CDS             complement(1718..2737)
                     /locus_tag="ACV5NJ_001823"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HHY6485906.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3370..4374)
                     /locus_tag="ACV5NJ_001824"
     CDS             complement(3370..4374)
                     /locus_tag="ACV5NJ_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HHY6485907.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4387..5436)
                     /locus_tag="ACV5NJ_001825"
     CDS             complement(4387..5436)
                     /locus_tag="ACV5NJ_001825"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HHY6485908.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5453..6274)
                     /locus_tag="ACV5NJ_001826"
     CDS             complement(5453..6274)
                     /locus_tag="ACV5NJ_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHY6485909.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6261..7040)
                     /locus_tag="ACV5NJ_001827"
     CDS             complement(6261..7040)
                     /locus_tag="ACV5NJ_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HHY6485910.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7057..7527)
                     /locus_tag="ACV5NJ_001828"
     CDS             complement(7057..7527)
                     /locus_tag="ACV5NJ_001828"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6485911.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7539..7958)
                     /locus_tag="ACV5NJ_001829"
     CDS             complement(7539..7958)
                     /locus_tag="ACV5NJ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6485912.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8039..10816)
                     /locus_tag="ACV5NJ_001830"
     CDS             complement(8039..10816)
                     /locus_tag="ACV5NJ_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HHY6485913.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         3116 a         2318 c         1786 g         3656 t
ORIGIN      
        1 tttaaaaaag attcttttat ataagtgaaa aaagcttttt tccaccaatt aaataaatct
       61 ctcatctcaa tagtttatcc attattttat ctttttttgc gtatacctac taaatattac
      121 cgtcttcttc tcgttcttct actttgttcg agagaatctg tactagaaaa agtagttgta
      181 tgtctacctt ttctgcctat ccgagacttt cttttcatat atgggatata aatgattaga
      241 tacagtacag caaaaaataa agcaaatacg caagcttccc aactacctaa cgccaattca
      301 accatcaagg tagcaacccc aacggatgct gcataccaga taaaaatcag aaacgtttct
      361 ttttttctga ccatgcgaaa catgataaat agactaataa gcaatagtgg caacacaaat
      421 atccaattgc ttccttccat cagccacttg agtatatcaa gcagcgactg tgcaacagcg
      481 actaattttc ccatagtgaa cacacacctt tttttataat taaaaaattc gcctttttat
      541 tctactatga atcttctggc ttggctttca tttctcttat gtaatcattt tcttttatac
      601 aacttaaaaa aaaattaaac cattagcata atattttctg aatctattca cgaaagtctt
      661 tttctatctt tcgttttcca cgactcaact gatctagagt caaaaaacta aaacgacagt
      721 tgcgcaaatg acgcacaagc tgccgcttta ataacaaata gcctttcatt ctcagcaggc
      781 cacttatttc cagttaactt tctgttctta gctttttact caactggata atatatgcac
      841 ttaatgaagt cccaatttcc gaatgttgaa gtccatattc aatatttgtc ttcatatatc
      901 ctaacttatc gcctacgtcg tatctttttc ctttaaactc tctcgcatag accgcctctg
      961 tctggttaag agtctgaagt gcatccgtta gctgtatctc gtttcctgct cctggttctt
     1021 gagtttctaa tatctcaaat attttaggag tcagtagata acgtccgata atggctaaat
     1081 tacttggtgc ttttcccatt tcaggttttt ctacaaaccc ttttactctg caacgaccct
     1141 tttcgctatc tgttttagga tcaataattc cgtatttaga tgtttcattt tctggtacac
     1201 gcatgacggc aacagcggaa gcttgtgttt cttcataatc catcatcaat tgttgagtca
     1261 atgggatatc atctttcatc aaatcatccc ccagcatgac gacaaacggt tcatttccta
     1321 taaatgcttt tgcttgaagc acggcatccc ccagtcctct tggatgagat tgacggatga
     1381 agtgaagatt gacttgagtc gtttcttcaa ctaaagcgag taattctgtt ttttcctttt
     1441 cccgcaaatt gttttccaat tcgatatttg catcaaagtg atcttcaatg ggtctttttt
     1501 cttttcccgt tacgatcaag atgtcttcga ttcccgaatc caatgcttct tccacaataa
     1561 actggatcgt tggtttatcc acgattggaa gcatttcttt tgccatggct tttgttgccg
     1621 gcaaaaaacg tgttcctagc ccagcggcag gaatgactgc ttttttgacc ttcacttgct
     1681 gttcctccta aacgacgatt aactattgat catctattta acgaagagaa aattcattct
     1741 ccatttttcc gtcacgtaac atgatttctt ttgctgcttt tttgatatct tctccattat
     1801 acaaaacatt atagattgtt tgagtaattg gcatctcaac acctagctgt tgagctagct
     1861 ctactgctgc ttttgtcgtt gccacaccct caacgaccat tcccatgttt tctagaactt
     1921 cttccagctt atgtccctgt cctaacaagt taccggcacg ccaattgcga gaatgaacac
     1981 ttgtacaagt aacgatcaag tcccccacac cactcaaacc gataaatgtc agaggattgg
     2041 cacccatcgc gacaccaaga cggctgattt ctgccaatcc tcgagtcatg attgctgctt
     2101 ttgcatcatc tccgaatcct aatccatgga tagcgccggc accaattgca atgatatttt
     2161 tcagtgctgc accagtttct acgccaatca catcattatt tgtataaatc ctgaaataat
     2221 cattcatgaa cagtttttgt acataggctg ctgctttttg atcaatactt gcagctgtaa
     2281 ttgttgtgat atcatgtact gccacttcct cagcatggct aggaccagat aatacaacaa
     2341 ttgcctgtct tttttcttct ggaatttctt cagccaaaac ttctgagatt cgtttatgcg
     2401 tatcttgttc tagtccttta cttgcatgta taatgattgg cttagtattc attttaccaa
     2461 taagttcctg tgcgactgaa cgaatcgctt ttgttggaac aacaaataaa atagcatctg
     2521 cgttatccac tgcttctgcc aattctttat atccgataat cgattctggg atcgtcaaat
     2581 caggtaaaaa atgctgattt gtatgatact tattgatttc atcaatctgt tcaggtacat
     2641 ttccccaaat acggacatca tgcccgtttt ctgccaatgt ttgtgctaaa gcagtgcccc
     2701 aagatccagg accaagaact gcgatttttt gtttcatgga tattcctcca aataaataat
     2761 tgataatttc cattatagta tacatttctt aaagttggga ctaatttcca ttgtttttaa
     2821 gtgtttgttt acttctttta tcgtttttac ttgaaataat caatgatatg ttacaatagt
     2881 ctacatttta tttttacact cgttatgctt tgcttgttcc taagaggaag cagttttgac
     2941 aatgttacta ccagcattac tattaggagg ttaggaaact tctcctagtg agtattgatt
     3001 taaaatacat aagagacaga ttgaacattt ctaaatctgt caggtcctgc aacggacaga
     3061 ttgtctctgt tgacggttga cataggaact tttgttttag tcaacattct ggatacaaaa
     3121 cgctatgtaa actggcgcaa ctcgttgtac ttggactttt actacggatc catgcaacaa
     3181 ctaaatagta ctattgcata acaaaaaatg gttggcaccc acatgtccca ttaaacagat
     3241 tataagttga actgtagata atgcaaaaaa tgtgttttat aacgtaattg atgccaatat
     3301 tttaaaaaaa taaagggagc agttatcgct gctctcttta ttttttatat tctttttatc
     3361 cagtctatcc taataatcaa actgacgata gtagcggcgt atctctaatg gatggcagtt
     3421 caactgctcc acatgagcat cgatgcgttg tgtcactaca tgcatcaata agaaagacaa
     3481 aacacgtccg cgatattttt cactgatacc ttctactgga tagtcttttg tgtctatcaa
     3541 tgtataattg ctgcaaattt ttggcaaaag tttagccaca cgttcagata acactctctg
     3601 ttcatcttcg cctaaaaata atgtgactgg ggttgtttct tctacgatct ctaacgtccc
     3661 gtgaagaaac tcggcagcat ggatagattt cgatggaagc cagctttgtt cttcccagta
     3721 acacatggca tatgaataaa cagctcccca ttgattccct gctccaataa aataatgcat
     3781 actatcacgt cgatgttttt ctgcaaatga taagccaaat gcatctgctt ttttctctgc
     3841 ttccactaac cctgttggta aataacgttc gagctgttca tagtattctg aataagcttc
     3901 gaattctcca ttcaaataca tcaatcgatc tgctgccata aaaaatttga tttgttctgt
     3961 tccaggagct ggataagtga ccacaaaatc gcatagctga gacaacttac tgttcgcttt
     4021 atcgataaat ccaatcagtg tcgctcctac tttcttgatt tgttctactg ctttcactac
     4081 ttcttgagtt gtaccggtaa cagaggacaa aataacgata gaatcttttg ttaatctttt
     4141 gtttccagta gtgtaatatt ctgcagcatg ctgtacaaag acaggcaagt tgcttttccc
     4201 attcatataa gtgacagctt gcatcgagga agcatacgtt ccaccgattc ctagataata
     4261 gacagcgtca aatttttccg cataaagttt atcgatgatt tcttctactt gcggacgtaa
     4321 agctagtgcg ccttttattg ctttgatttg ttcttcttca ttgaatttca gcatgatttc
     4381 cacctcttat aatttacttt tcaagactgt atcaaagtct tcaaaaatct ttttgttcaa
     4441 gtcaatacct ttagctactg cggttttata agcgaatagt tgaaacggaa cgacaagcgc
     4501 taatacagac agatattcat cacattctat tgctaatcca agtgttttct cgtttcttgc
     4561 tttttttgtg gtaatggtga gagtctctcc aacgtaccga ctcatatatc tttgcaactt
     4621 ttgcgaacgt tccttattta cactttcccc ttcaataaag aatagcaaat gcgaagcatc
     4681 tgcctccagg tatgggccgt gcatataagc ttctaactca aatccttgtg acggaacgcg
     4741 gattgtttca gtcaatttcg tctctgcttc tttagctgtt ccccagtttg gaccatatcc
     4801 aatggtgacg aaacgctgtg ctaaacgaaa taaactttga tatccatcaa aaaattcttc
     4861 cgttttttga ataatcgctg gtaaatggtt gattattttg cgcaactgtt gcatgtagtc
     4921 ttgttctatt tttttgctga ttttattttt acttattcca attcctatcc ctaagagtag
     4981 taactgtaaa acagtcgcag aatagccttt tgtaacgaag cctactgatt ctaccccggc
     5041 acctaaatca ataatttgtg cagcttctaa agcaagtgga ctttgcacgt catttgtgat
     5101 agcaacaaca ggtaaccctt gtttttgtat catttttact gcttcaacag tagaagcact
     5161 tttcccgctt tgtgacacag caatcacagt atcgattgaa ggagaaagtt ttccataatt
     5221 gaaaaaataa aaaggttctt gaatatcgac agtaagatca ccataagatt ccaatgctgg
     5281 cttcgctgct aaacaagcgt tataagatga acccgttgct aagatcagta aattcactgt
     5341 ttttttcata tgttctcttg tcgaaaaatc attctttttc aagatcgtac ttagtacttc
     5401 cttttcttca tagatataat cttgcatcgt aaacattttc tctgctcctt ttttaaaaaa
     5461 tccctatcca cgaaccaaaa attccaaaga aagctagtcc gatcaagatc gttaatggtt
     5521 tcacttcttt cttcaataga taaaacacta tgccaaatgc tccaagacat aagatatttg
     5581 gaacgatatc atcaaaaata ctttgaacag tcacagcatt ttctcctgaa ccgattttta
     5641 aaggaatatt gatggtgatc atcgtggcag tcattgctcc aacgaccatc agtccaataa
     5701 tagacgcgcc taatgtcaaa ctttccatca taccgtttgc ctggatcttt ttcaaaaaac
     5761 ccgttcctaa gcgatatccc caacctgttg ctagatagcg aaacagaatg tgcggaatat
     5821 tgaaaatcaa aatgaacaag atagggccta aaatatttcc ttgcatggct aaagaggtac
     5881 ccacacctgt ggcaatcaat cgcaaagttc cccaaaagaa agaatctcct aaccctgcaa
     5941 gaggtcccat caaagatgtt ttgatactat cgatcgcagt cacatcaaat tcaggatcat
     6001 ccttattttc ttcctccatt gcagccgtga ttcctaaaat cagtgtaacg atatgtgggg
     6061 tcgtgttaaa aaactctaga tgccgtttca aagctgctgc catttcttct ttcgactgat
     6121 ataacttttt caaaatcgga atcattgcat aaacataagc catattcatc tgacgttcat
     6181 aattccaaga aaactccatt tgaaaagaac gccaaaatac gtgatttagc tcttttttcg
     6241 ttattttgct gtttttgatc tcagaattct tcatcttctt ctacctcccc tgcagttgtt
     6301 tgaagtggta gttgacggac attcataata ttgactacca caactgctaa aatcgcacca
     6361 aaaatagcta ctccggtcac aggaatctct aagtaagaag ctatagcaaa acctaagaaa
     6421 aaatatggtg caacttgttt gttgatcagc aaacgagcaa gcatcgcaaa accaagagct
     6481 ggaatcaacc ctgtagctac tgacaacccc ttttgaacga agtcaggaat catatttaat
     6541 agatttccga tcgtattgct tcccaccata taagaaacca ttaccacaac accaagcatc
     6601 aaggaaagac cgataccggc aagcaagtgc atatgctcga ctcctttata atttccttct
     6661 tctgcataat tgtctgcttt atggttcata attggaatca aaagccctaa gtaaatattt
     6721 ttcaatatca atgtcaacgt agcaatcggc aaacctagta aaagcgcggt ttctgtaccg
     6781 gctccagctg taatagcaaa agccgtccct aaaatcccac cggtcacaac atcaggtgga
     6841 atcgaagcac ctactgagaa cgatccgata aatgctaact ctaaagttgc gcccataatg
     6901 atcccagttt ttacatcacc taacacgatt cctgtaaaca gccccgtcac aatcggacga
     6961 gaaagcaacg atgtccctag tgcatattct gattgtgcga caaatgcaac aattcctaat
     7021 aagactgctt gtacaatcat tgaggttctt cctttcttac aaaacttctc ttacatccac
     7081 ttttttatct gctggtactt gacggatttc tacttctatt tgtttgtcaa ttaatcgttt
     7141 caagcgttcc tcctcttctg gtaaaatatt taccgctttt ccgatgctgc gagtaccagg
     7201 ttttgccttg atccctccta agttgatggt tttgatttct ggatacgctt ctgccagctt
     7261 atatgcatcc tcgatcgatt ccactacgat gaataactta tatttatctg tcaccccgct
     7321 tttcaacgcg gcaatcgagt catcaattga tttgatcact agtttgactc cttgtggctt
     7381 ggctaatttg atcgttgtct ttcgtatctc attcgtaggt acatcatcat tagcaatcaa
     7441 aatacagtct gcgcctattg tttgtgtcca tgaaaaagct acttgtccgt gtaataaccg
     7501 atggtcgact cttgtcaata caatcatttt tcttcctcct aaaattcgtc ttcttctatt
     7561 ttctgattga gccgctcgtt acaaaactgg atcatttttt ttgaatcagt tagcacctgc
     7621 tggatcaaaa cagttgtctc tacttcagcg tccaaccgag atactagctc aatcaatagt
     7681 ggcaaattca atccggcaac gagatagaca tgatcccgat tgatatattg taagaattcg
     7741 ttgttgacac ttccgccaaa aagatccgtc agaacaatga tttctttatc tttgtacctc
     7801 ataaaaagct gccttactgt ttccgctaaa tcaaaattct catcagtata gcaatcgaat
     7861 acagtaatag aatgattttt cccatagatc agctctaatg aatctaatat ccctgaagca
     7921 aattttccat gggaagctaa gataatttct ctatccactt tttcatctcc tcttttcact
     7981 tacacatact aaaaaagcaa gttgcatgcc aaaattagtg gcaacaactt gcttatgttc
     8041 aaaattcttc ttctatcgag agaaactctg tatcttcact caaaatatca tagatatagg
     8101 caatctcaga attggggatc atgacactat aatgctcttc aatgacacta aacgcagatt
     8161 taatagcaga taacccttct ttttgacact gcttccattt ctctaatcct tgataggttt
     8221 cgattggcat ttgtcggatc aatcgttcaa tgagacagct cacatggaca tacaacgcta
     8281 attttttggg attgctgatg cttcgattca actgcacctc taattctctc ataaaatgat
     8341 cgatttctcc catcaccttc tctgtatcta gaatagtcac ggaatccagt actttttcca
     8401 atgaaaaatt acgaatgatg cgctgattga atatttcctt ctctgaagca ttcatagtag
     8461 tgtccaacca ttcagctagt acagacatct tttcccccga gatgaggtct tctaatgaaa
     8521 gatagtcgat attggtgata tcaggatctg tggtgccaat cattcctacg acttcgtata
     8581 tggaaaataa tgggtcactg tttttcttat cttttaattg ctgatattca tatgggatga
     8641 ttttcaactg acaagccgta ggtaagcttt tttctagcag ttcacttaag tggaccgccg
     8701 taccgattcc agtgaagcaa gtagtaatga gtgctttggg tttgttttcc tctggataga
     8761 gcagtcgcca ttctggctgg atctgtcgaa cagtctcttc tgctatctct tctaatgata
     8821 catttttttg caggcattcc cctactgtga ttgctaaagg tgtcgtcaca ttattcaaaa
     8881 tcaaaagcgg tacggataac tgttttttaa aaaactgatg gatttctttc agagatccca
     8941 tgtctactaa tatgatcaat ccatttgaaa catcgctgtt ttcgcaataa tcaaggattt
     9001 ctgaagcaat ctgctgtggt gtgacatcta gtggcatatc aaatgattca aatagatcct
     9061 cttgtaataa acgatttact acatttgcca tactgctagc agttgcataa ccgtgagcag
     9121 caatcatcgc ttttggcagt cgtttttctt ttgaccattt cgcttttttt agatagatcg
     9181 tcagtaaaat ccgatccata cttgtgtctt caatatctaa tttgatctta cttaattcca
     9241 gaattcgttg gacatagtga taactagatg gataagactg agaaacttgt tgttctaatt
     9301 ctttgattag ttggataata tcatgttctt ccggtaacca tttgatagtg cttcgctgat
     9361 agagatagta actcaaggca tagacactgt ttccatcgaa tttgatttga taggcagttt
     9421 ccatttgtcg aaaggtctct cggatagatt gtgtccaata aaccagcagc tcgtgttttt
     9481 cttgtctact ggtctcaaac agcaaaaaat caaataattg aatcacttct tgctttaatt
     9541 ttccttcgca agaatatagc agacagttat ttttcttgaa atcaattagg atttgctcaa
     9601 aacttttaac gattcgctgc tgttcgggct gttgcttctc cattaattgc tctaaggtct
     9661 ggtcttgcgt aatatagatt ccttgctgtg ctgtattttt agtaataggc tgcgcaagta
     9721 gttctttttg caaatgatgg atcgtgacat ggatttcagg ctgttcacgc tgttcagcat
     9781 atgcttttgc tgctgaaact ttgacgatat ttttcagctc tcctatgttt ccggataatt
     9841 tctgacttgt taaaagtgac aaagctggtc cactgactga caaagggctc cccatttttc
     9901 tttgttcttc gagaaaaaaa gagtaaatca gttctagccg ttcattacga ctacgctgat
     9961 taagtgcagg tagttcgatt cggataggta ttcttcgaat gaaagttgtt aagaaatgac
    10021 tagtaatctc ttcagtcgta gcaaaaaaga gacgtgtttt gatccggcgt gacaaattag
    10081 gttctcccat acgataaatc acaccttgat caagataggt gaacagtttt tcctgacctt
    10141 ctggtgttaa gcgatgtacc tcgtctaaaa acaagatccc accatctgct gcttcaaaag
    10201 cccctttttt attttcttct gcaccagtgt acgctccctt aacatgtcca aacaaattgc
    10261 tagtcaaaag ttcaggatta tctgcatatt gtgcacaatt aaaagtgatg aatggtgcag
    10321 aatcttctag cagatcatga agcagacaat actgatagac caaattaact aaataacttt
    10381 tacctgtgcc actttcgcct gtaatcaaaa gtggtaatcc gccatctgga tagtaaagag
    10441 ctgttttgat ttgttcgata ctttctttca aactggcgtc gtatccgatc ataaaagaga
    10501 ataagtcttt tggttgcgcg aaaaaaggct gttcctcttt gagatcatcg atagaaaaat
    10561 aaaaattcgt gcgtaactta aaaaattgtt tttcaaaagc ttctttgtgg aaataaatca
    10621 ctggtcgaga attgattttg actagtagtt gctcatcatt taactggttc aaataatgac
    10681 tgactgtgtt tctttttacc ccaaaatgat cagctaacct ttgtgctgta aagacctcgt
    10741 tcatatgttc taaatcgaaa aaggctgtct ggttctctaa gtaacataaa atttccttct
    10801 tcaacaactt attcacccct cctccatcat agaaaacgga ttcattttgt caataaaatc
    10861 tttaataaag aattga
//
LOCUS       DBKOIJ010000091        10784 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000091 DBKOIJ010000000
VERSION     DBKOIJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.093"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            61..1320
                     /gene="lysA"
                     /locus_tag="ACV5NJ_001831"
     CDS             61..1320
                     /gene="lysA"
                     /locus_tag="ACV5NJ_001831"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HHY6485914.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1307..2107
                     /gene="dapF"
                     /locus_tag="ACV5NJ_001832"
     CDS             1307..2107
                     /gene="dapF"
                     /locus_tag="ACV5NJ_001832"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HHY6485915.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2110..2991
                     /gene="dapA"
                     /locus_tag="ACV5NJ_001833"
     CDS             2110..2991
                     /gene="dapA"
                     /locus_tag="ACV5NJ_001833"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HHY6485916.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2988..3704
                     /gene="dapB"
                     /locus_tag="ACV5NJ_001834"
     CDS             2988..3704
                     /gene="dapB"
                     /locus_tag="ACV5NJ_001834"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HHY6485917.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3710..4405
                     /gene="dapD"
                     /locus_tag="ACV5NJ_001835"
     CDS             3710..4405
                     /gene="dapD"
                     /locus_tag="ACV5NJ_001835"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HHY6485918.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4421..5422
                     /locus_tag="ACV5NJ_001836"
     CDS             4421..5422
                     /locus_tag="ACV5NJ_001836"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HHY6485919.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5431..6780
                     /locus_tag="ACV5NJ_001837"
     CDS             5431..6780
                     /locus_tag="ACV5NJ_001837"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HHY6485920.1"
                     /translation="MKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGKRTSD
                     DDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQKQLED
                     EKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITKAFKR
                     YPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMADPRI
                     IDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGTKISN
                     HEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPSGIDN
                     IGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSGKVLS
                     ILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTPSNS"
     gene            6740..7891
                     /locus_tag="ACV5NJ_001838"
     CDS             6740..7891
                     /locus_tag="ACV5NJ_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HHY6485921.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7866..9035
                     /locus_tag="ACV5NJ_001839"
     CDS             7866..9035
                     /locus_tag="ACV5NJ_001839"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HHY6485922.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9210..10589
                     /locus_tag="ACV5NJ_001840"
     CDS             9210..10589
                     /locus_tag="ACV5NJ_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHY6485923.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3576 a         1895 c         2425 g         2888 t
ORIGIN      
        1 aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa
       61 atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca
      121 cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc
      181 tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt
      241 gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa
      301 atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac
      361 aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat
      421 aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt
      481 atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg
      541 catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag
      601 ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa
      661 atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat
      721 tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat
      781 gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct
      841 ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt
      901 ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa
      961 tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa
     1021 tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca
     1081 ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa
     1141 tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac
     1201 tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt
     1261 gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa
     1321 atataatggc tgcggaaatg attttatctt attagattac gcagaagaca cagattatag
     1381 tcaattagcc gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt
     1441 aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg
     1501 cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca
     1561 ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc
     1621 tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa
     1681 tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac
     1741 tagtttattt ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt
     1801 ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt
     1861 caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt
     1921 aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac
     1981 tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc
     2041 tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga
     2101 ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga
     2161 tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat
     2221 ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag
     2281 cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct
     2341 ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca
     2401 gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct
     2461 cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct
     2521 agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc
     2581 attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt
     2641 ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta
     2701 ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta
     2761 gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt
     2821 gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca
     2881 ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa
     2941 aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac
     3001 tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata
     3061 cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc
     3121 cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct
     3181 tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag
     3241 aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca
     3301 tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg
     3361 aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc
     3421 tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa
     3481 aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac
     3541 atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta
     3601 atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag
     3661 gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca
     3721 agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa
     3781 tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt
     3841 tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat
     3901 tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa
     3961 aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa
     4021 tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat
     4081 gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg
     4141 tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga
     4201 agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga
     4261 tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg
     4321 tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt
     4381 gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg
     4441 tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga
     4501 ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt
     4561 attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga
     4621 tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag
     4681 tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt
     4741 aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa
     4801 ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca
     4861 gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga
     4921 tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac
     4981 ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat
     5041 gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt
     5101 acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt
     5161 tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga
     5221 tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt
     5281 tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga
     5341 taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa
     5401 aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg
     5461 atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc
     5521 caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac
     5581 ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa
     5641 aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa
     5701 gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga agattattta
     5761 gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt
     5821 attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca
     5881 gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac
     5941 ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc
     6001 gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga
     6061 atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt
     6121 agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc
     6181 ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa
     6241 gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga
     6301 cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt
     6361 atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca
     6421 gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc
     6481 atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta
     6541 gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg
     6601 ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag
     6661 ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat
     6721 gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga
     6781 aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga
     6841 ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga
     6901 agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct
     6961 ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc
     7021 gatgctttta gggttgactg actttataac taaacacatt gaggatctta tttataatgt
     7081 agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc
     7141 aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact
     7201 agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga
     7261 cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct
     7321 ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga
     7381 gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc
     7441 tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt
     7501 gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg
     7561 ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga
     7621 ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata
     7681 tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca
     7741 tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac
     7801 gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt
     7861 aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa
     7921 aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg
     7981 gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag
     8041 aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta
     8101 tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag
     8161 ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg
     8221 ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg
     8281 aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac
     8341 gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc
     8401 ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat
     8461 atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata
     8521 tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac
     8581 atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac
     8641 taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg
     8701 ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac
     8761 gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg
     8821 gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt
     8881 gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag
     8941 aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct
     9001 gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa
     9061 tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg
     9121 aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt
     9181 ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg
     9241 catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag
     9301 atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt
     9361 atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc
     9421 cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa
     9481 aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat
     9541 tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa
     9601 atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc
     9661 cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag
     9721 caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca
     9781 cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg
     9841 aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca
     9901 aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa
     9961 atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg
    10021 aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt
    10081 ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac
    10141 gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag
    10201 gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta
    10261 cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa
    10321 aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa
    10381 tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa
    10441 atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact
    10501 tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag
    10561 cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa
    10621 aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
    10681 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag
    10741 ggactgtgac acgatttgtg taccgacccc caaaagttag acca
//
LOCUS       DBKOIJ010000092        10667 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000092 DBKOIJ010000000
VERSION     DBKOIJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.094"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            79..894
                     /gene="thiD"
                     /locus_tag="ACV5NJ_001841"
     CDS             79..894
                     /gene="thiD"
                     /locus_tag="ACV5NJ_001841"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HHY6485924.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1223..2674)
                     /gene="cls"
                     /locus_tag="ACV5NJ_001842"
     CDS             complement(1223..2674)
                     /gene="cls"
                     /locus_tag="ACV5NJ_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HHY6485925.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3015..5681
                     /locus_tag="ACV5NJ_001843"
     CDS             3015..5681
                     /locus_tag="ACV5NJ_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HHY6485926.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6045..6797
                     /locus_tag="ACV5NJ_001844"
     CDS             6045..6797
                     /locus_tag="ACV5NJ_001844"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHY6485927.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6819..7973)
                     /locus_tag="ACV5NJ_001845"
     CDS             complement(6819..7973)
                     /locus_tag="ACV5NJ_001845"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HHY6485928.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8158..10599
                     /locus_tag="ACV5NJ_001846"
     CDS             8158..10599
                     /locus_tag="ACV5NJ_001846"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HHY6485929.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3475 a         1905 c         2292 g         2995 t
ORIGIN      
        1 gatactttga aaaacatatg atagaaacag taacagtatt atgaaaaatc tttttataaa
       61 aatggaggaa tcgatttgat ggaagatcag tatgtacaag caatgacgat cgcaggatca
      121 gatagcgatg gaagcgcagg gatgcaagca gatatgcata cattctttac aagaaatgtt
      181 tacggtgttt ctgtcatgac cgcatgtgtt gcaggtaatt cttacggaat cggagcaagt
      241 gtgacattgc caacagattt tatcgacaaa gaatttgaac tgattgcgaa ggactttcaa
      301 atacgtgcag ctaaaacagg tatgctagca gattcaaagt tgattgagac agttgtaaaa
      361 aattaccaaa aatatgattt tggaccatta gtcgtggatc ccgtaatcgt aacgaaacat
      421 ggaaatcttt tactagaaga atctgctttg caaagcttga aagaaaagct tgtccctttg
      481 gccgaagtac tgacacctaa tttttatgaa gcagaaaagc tgactggacg gacattagca
      541 gctgaagaag attttgtgca agcagcaaaa gaactgcaag atatgggtgc aaaaaacgtt
      601 atgatcaaag gacatcatgc taaaaatgcg cagcaagaaa cagtcaatga ctacgtattg
      661 ttcgaatcag gaaaatcatt ttggttaaat gcaccatttt atcctaccga ccggatcaac
      721 ggaactggcg attcgctatc tgcatgtatt gcagctgagt tagcgaaagg aaaaacagtt
      781 gaacaagcga tccgattggc aaaagactat gtaaaccgtg caattcgtga agaaataaat
      841 gtagggcaca aatatggccc catcaatcat tgggcagctg gtgaattaga gtaaaagaaa
      901 gaagttgtga aaaagttgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc
      961 ttatgttttt aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttaa
     1021 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
     1081 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
     1141 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
     1201 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
     1261 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
     1321 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
     1381 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
     1441 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
     1501 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
     1561 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
     1621 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
     1681 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
     1741 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
     1801 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
     1861 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
     1921 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
     1981 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     2041 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     2101 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     2161 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     2221 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     2281 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     2341 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     2401 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     2461 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     2521 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     2581 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     2641 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     2701 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     2761 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     2821 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     2881 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     2941 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     3001 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     3061 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     3121 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     3181 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     3241 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     3301 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     3361 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     3421 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     3481 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     3541 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     3601 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     3661 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     3721 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     3781 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     3841 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     3901 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     3961 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     4021 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     4081 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     4141 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     4201 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     4261 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     4321 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     4381 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     4441 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     4501 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     4561 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     4621 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     4681 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     4741 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     4801 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     4861 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     4921 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     4981 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     5041 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     5101 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     5161 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     5221 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     5281 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     5341 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     5401 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     5461 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     5521 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     5581 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     5641 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     5701 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     5761 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     5821 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     5881 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     5941 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     6001 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     6061 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     6121 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     6181 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     6241 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     6301 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     6361 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     6421 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     6481 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     6541 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     6601 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     6661 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     6721 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     6781 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     6841 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     6901 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     6961 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     7021 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     7081 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     7141 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     7201 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     7261 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     7321 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     7381 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     7441 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     7501 acagcaccga caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca
     7561 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     7621 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     7681 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     7741 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     7801 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     7861 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     7921 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     7981 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     8041 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     8101 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     8161 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     8221 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     8281 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     8341 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     8401 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     8461 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     8521 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     8581 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     8641 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     8701 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     8761 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     8821 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     8881 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     8941 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     9001 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     9061 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     9121 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     9181 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     9241 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     9301 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     9361 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     9421 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     9481 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     9541 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     9601 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     9661 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     9721 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     9781 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     9841 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     9901 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     9961 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
    10021 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
    10081 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
    10141 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
    10201 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
    10261 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
    10321 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
    10381 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
    10441 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
    10501 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
    10561 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
    10621 ataaagcaag atttacagtt attttattta ctgcagatct tgctttt
//
LOCUS       DBKOIJ010000093        10654 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000093 DBKOIJ010000000
VERSION     DBKOIJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.095"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            181..2241
                     /locus_tag="ACV5NJ_001847"
     CDS             181..2241
                     /locus_tag="ACV5NJ_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HHY6485930.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2324..2893
                     /locus_tag="ACV5NJ_001848"
     CDS             2324..2893
                     /locus_tag="ACV5NJ_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6485931.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3068..3874)
                     /locus_tag="ACV5NJ_001849"
     CDS             complement(3068..3874)
                     /locus_tag="ACV5NJ_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485932.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4012..5526
                     /locus_tag="ACV5NJ_001850"
     CDS             4012..5526
                     /locus_tag="ACV5NJ_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HHY6485933.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5929..6912)
                     /locus_tag="ACV5NJ_001851"
     CDS             complement(5929..6912)
                     /locus_tag="ACV5NJ_001851"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HHY6485934.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6917..7564)
                     /locus_tag="ACV5NJ_001852"
     CDS             complement(6917..7564)
                     /locus_tag="ACV5NJ_001852"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HHY6485935.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7649..8281)
                     /locus_tag="ACV5NJ_001853"
     CDS             complement(7649..8281)
                     /locus_tag="ACV5NJ_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHY6485936.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8297..8848)
                     /gene="coaC"
                     /locus_tag="ACV5NJ_001854"
     CDS             complement(8297..8848)
                     /gene="coaC"
                     /locus_tag="ACV5NJ_001854"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HHY6485937.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8845..9582)
                     /locus_tag="ACV5NJ_001855"
     CDS             complement(8845..9582)
                     /locus_tag="ACV5NJ_001855"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HHY6485938.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9579..10568)
                     /locus_tag="ACV5NJ_001856"
     CDS             complement(9579..10568)
                     /locus_tag="ACV5NJ_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HHY6485939.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         3443 a         2009 c         2112 g         3090 t
ORIGIN      
        1 tattacttaa caaaattaaa aagtacataa gttattattc cgattttctt gaaacaataa
       61 aaaatcaaaa aaaatactct cctaatagtc catcttcttt tgattttatt tatataatga
      121 aatcatctta tgaaaattac tacttcatgt ttagacaaga gtagggagga ctaagaataa
      181 atgtgttcaa aaagacgttc aaaacaacta aataaacacg cttgtcgttt agataaggaa
      241 cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc gcacgatgct gatcagttta
      301 tctgtaggtg tctatatgca tgtacccgtt caggcaacga cacaaaccat caaggtcgta
      361 cgtacctctg tttctgagac cactgattcc cctatcacta ttactcatac tttcactgat
      421 gcaaatggaa aacagctact tcctgcattg acttcctata ataaagtcag ttatcctcca
      481 acgataaacc actactggtt aattcctaac aaaacattat ggacatcgga aaccggaagc
      541 agtgtgccgc tttctatctt ttccgatatg actgatccta caaaaggtga tcagttaaaa
      601 cagatcactg atattttgaa tcatgctttg acagatataa aggacggtgg aagcgcacaa
      661 gtttcttatg tatatgaaaa agattattca cagtttgaaa gaaagaaaat cgatgttctg
      721 gcagggacaa aagtttcgat atctgatctg gtagaaaggc tgaagaacgc agatggaaca
      781 caaggagata tgtccaaagt aatttcaaac aatgggaata tcgtggatac gactaataca
      841 ggaacatcta ctatttcctt gtcctatttt gatccaattg ctttaaaaac aatgactgca
      901 tctgctgtga tccatgttcg tgctgatcaa acagctatca aaggaaaaga tcttcactta
      961 agaacaggag agagttacgc ccctaaagat cttgttgaat atgtaacaaa aagcgatgga
     1021 acttcaggaa ataaagaaga aatcaaaatg aacgggacag tagatactac aactccggga
     1081 gaatatcccg ttgtattaac ttatatagac tcgaaaacat caaatgtggc agaaacaaca
     1141 gctgtagttt atgtagaagc atctgctgct tcatcgactg tcatcactca ttttatagat
     1201 agtcaaacaa ataaagaact tcaagcagaa acacatatga gtagtgtaaa tcgtattcct
     1261 gatttgcgcc tacctgctga attttctgat cgagtgccaa atatagattt atcgacttat
     1321 gaacaaaatg ggaaagtcac ttctatttct gattggatgc aagcaaacgg attagacaga
     1381 aaaagtcact ggtcggagat ccttgaagta cttcactcag aactgatgaa taataggaag
     1441 caatcaggag gggaaattac ctttacttat gtttatatac cagacaatcg ttcattagaa
     1501 ggtcatgatc ttattgttca tccaaacgaa aaaataaaca aagaagagtt gatccaatca
     1561 gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg tatcggttac aatcaatgaa
     1621 gtaccattgg aggatggtta tttgaccaaa caaacaggtg attttacgat tatgtatagt
     1681 tatcatgacc cgtattcact ccaagaactc catgctcagt cactattaca tgttttacca
     1741 ttaaacaaac agatagatca aaaagaacaa aaaagacaag aagaaagctt gtcagaaaaa
     1801 aaacaacaaa tggctcatgc agtttctcca acagggaaag taaaacaaac aagaagccta
     1861 gagttagcaa gaatatctag aaaaatctca gaatctaata aaacgataag gcaagaagct
     1921 catttggccc cacaagtttc tggattggaa aatgaaattt tagagcctaa aataaaacaa
     1981 caatcagaaa aagaatttgt tgctaaacag tcggaagatc agaaggaatc agatgaccag
     2041 aaggaaacga agaaaaaacg cttgtcaact attgattcca ccgatgagca tctgcagccg
     2101 caagtaccac ttcctagtcc gggaacaccc gcacctggtg gaggtattcc ttcttctgct
     2161 ttaggagatt tcatgcgagg tatggcagga acatgggtat acgctaatcg aaatgaattt
     2221 gacgcagatg actggttctg atttatatct ctatctgtta tagtcaaata agtagctgag
     2281 tcatatgtta tttggcataa tggagacgag gagggataga aaaatgttag ataaaacgtt
     2341 accttacgca gaaatctgga tgacaagacc acttcaacaa aatgttccaa tcacgccttt
     2401 agcggaaggt tttgaatttc gattttatca aaaagaagat gaatctgctt gggcggagat
     2461 tgagacttcc gttggggaat ttcaaacgac agatgaagca cttgagtatt ttcaacaaaa
     2521 ctttgctcct tatcctgaag aacttgaaag aaggatgttt tttgtagaga caaaaaaagg
     2581 agaaaaagta gcaacctgta ccgcttggcg aaaaaaatca agagagcaag tgtatccggt
     2641 ttttcattgg ttagcagtga aaccagatta ccaaggaaaa gggctcgcaa aagcattgac
     2701 tgccagagtc ttgcaagaat ttcctaccct ccatacggac ggaccaatct atcttcacac
     2761 acagacatgg agccatcagg cgattgctct ttataaaaaa atgggtttta cttttattgc
     2821 tgaaaatcta gacggcacac ctaatccaga ttatgaaaaa gttatgcagg ttttaagtaa
     2881 attagataaa taacgaaatg gtgaaataag tcctcagtat tgggataaaa aacgaaatag
     2941 tcattcctca tatcttactt ttttgagtta atactatcgg ataactaatg gcatgccata
     3001 cattcaaaaa agaaggggca gtgacaaaag tattgtcact gccccttctc ttgtttttta
     3061 gcatgtttta tcgtttgatt tttttaggaa taaaattctt gatgaacgta aatttttggt
     3121 aatcgtttcg actatagagg taaccaaata cactgaaaac aactgctcca ataaaattaa
     3181 caaataaatc cttcatagta tccatgattc ccacgtctaa gtaaccattt tggatgattg
     3241 ttttatctgt tttaccatct gcattttgac tttcaatgat tgtccgagtg atatctggaa
     3301 catgatagac agtgtttccg tctttactta actccacact attgatttgc tggatgatcg
     3361 tatctttttg catatcgaat tgcagtaatt tatctgctga aaattccaca aactcccaca
     3421 taacaccaac tgtcatcgaa aaacagaagg aaacaactgc tagtaataaa ggagtcaagt
     3481 tcatggtttt tgtattttta ttgagcaaat aaacaaggga aaagccgaca cccccagcta
     3541 aaaatccgct gaatgcatgt agaaaagtat cccacattga aaaatagcca taaaaatcac
     3601 ttaactctcc taaaactgtt gaactaaaaa taaaagataa caaaatgatt tctagcatat
     3661 ttggaatctg tatctttaat atcttttcca gaaaataagg gatttcaaat aaaattaatg
     3721 tcagtaataa aagcattgta tttccccagt gttggttcca gacctgatcc actagcataa
     3781 taacgacgat gatacgtaat aaaatataga ttccgtttgt gattctacct gcagtttttg
     3841 tactgttcgt aaaaaaggtt ttcaaatgat tcataaaata actccttcat gtatatcctt
     3901 aattatacta agaagcgtgt gtttaccaaa gctatatgac ttaaagaaaa acagagttga
     3961 tattgtgaaa aaaatcactt aatatagtat aaggagaaaa gggggataca aatgaaaaaa
     4021 ttagttgctg gtttattaac agccgttgca cttcttgcta tgagcgcatg cggcgcagat
     4081 cagaaaaata cagcgcagac aagttctagt acgaaggaat ctgccgaagt atcatcaggc
     4141 gcttctgaac aagtatatac agatccaaaa gaaatgaaag aatcttatga tgtcgtgatt
     4201 attggagctg gtggtgcagg gatggcagca gctatcgaag caaaagatgc tgggttgaat
     4261 ccagtaattt tggaaaagat gcctgttgcc ggaggaaaca catctaaatc atcaagcggt
     4321 atgaatgctt cagaaacaaa atttcaaaaa gaagaaggaa tcaaagactc gaatgatgcc
     4381 ttctatgaag aaacgctaaa aggtggtgga ggaacgaatg ataaagaaat gcttcgatac
     4441 tttgtcgatc actctgctga tgcggtcgat tggctagatc aaaacggtat caaactggac
     4501 aacttaacga tcactggagg gatgagtgaa aagaggacac atcgtccgtc tgatggctcg
     4561 gctattggtg gatacttagt ggaggggtta ttgaataacg tccatgagcg ggaaatccct
     4621 atttttgtca acgcagatgt gacagacatt aagaaaaatg gggaaacggt caatgaagtg
     4681 actgtacatg ttcaaggaga agaaccaaaa gaggtcaccg gaaaagcaat cgtagttacc
     4741 acaggcggtt ttggtgccag caaagaattt attgaaaaat atcgtcccga tttagctgat
     4801 tatattacga caaatcaaga aggatcaact ggagatggta tcaccatgat tgaaaaatta
     4861 ggtggacaaa ccgttgatat ggataaaata caaattcatc caactgtgca gcaggatgag
     4921 ggggtcttga ttggtgaggt tgttcgcgga gaaggagcaa tcttggtcga tcaagagggg
     4981 aaaagatttg tcaatgaaat ggatacaagg gataaagtat ctgcagcaat tacagcattg
     5041 cctgaaaaat cagcttattt gatttttgac caaggcgtgc gtgatcgtgc cacagctatt
     5101 gaattttatg accaaaaagg atatgtcaca actggaaaga caatggaaga attagcagaa
     5161 aaaatcgatt taccaaaaga aacactagca aaaacagttg atggatggaa tacggatgtt
     5221 gcggagaaaa aagatgctca attcaaccgt gccacagcca tggaacatga cttatcgtct
     5281 ccgaattatt atgcgatcaa aatcgcacca ggaatccatt acacgatggg cggtgtgaaa
     5341 atcaatacga acactgaagt attagataaa gaaaaccagc caattagggg gttatatgct
     5401 gctggtgaag tcacaggtgg cttacatgga aacaaccgga tcggtggaaa ttcagttgcg
     5461 gagatcatta tttttgggcg acaagcgggc atacaagcag ctcgatttgc ttcggaaaat
     5521 gaatgaaata gaacgtaaat aaaaagaggt tgtgaaatca gcgttttgag ctaataccga
     5581 gggtcagctg attgaacact gtttagtgaa gaggttgtga aaaagctgcc ctgcatcaag
     5641 taataagaac agccaagtaa aaacagcttc ttatgttttt aacttggttg gacttattac
     5701 cgcagatgca agcttttgaa caccgtttat tcggaaaaag agcgtgggac aaaagtaaag
     5761 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaaga aattccttcg
     5821 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     5881 ttggagttaa acgcttctgt cccagcctct tttttgatca gcatgatttt attcagttat
     5941 cttcaaaatg atttttccga tgtttttgtt tgcttccata tattcatgcg cttgtttgac
     6001 tgcgctgaat tggaatgtac ggtcaatgat tggccgaatc ttgccatttt ctagaaaagg
     6061 cccagctttt tgcataaaag tttgtgcgag ctctgcttta tactgatcac ttctaggtgt
     6121 caataatgta ccaagcagtt ggacacgttt cccaatcaat acaccaaggt ctatctcagg
     6181 cacgatggtc ccacctagca taccaatcaa gacccaacgt ccatcaacgg ctatagaagc
     6241 taaattcttt tcccagtaag aagcgccgat gaaatccaaa ataacatcga ctccccgacc
     6301 ttcagtgatt tttcgtacct cttcttgaaa gtcttgtttt ttatagttga tcaattcatc
     6361 tgcaccaagt tctttacaaa aggcgagttt ttcttctgat cctgcagtaa cgatgatttt
     6421 cgcgtgagaa agctgtctag ctaattggat cgctgctgtt cccacaccac ttgctcctgc
     6481 atggatcaag acagtttcgc cttcttgtaa ttttccaagc cagtaaagtg tttgataagc
     6541 agtcaaaaaa acttctgaga ttccagcagc agaaacatag tctagcgtat cagggagaag
     6601 gattgcacga tcggcaggca tcacagcata ttcggcataa ccacctagat tgactaatcc
     6661 gtatactttt gtcccaggag taaaagtgga gatatcactt ttattctcaa ctacctctcc
     6721 agctacttcg actcccaaaa tagggtaggg aggtttcaaa gagactgatt ctcttttcat
     6781 aatatctgtt cgattgacag ctgctgtatg gactttgatc agtaattcgc cttgttttgg
     6841 tttaggaaca ggcacttcaa tttgttctaa tacatctaca gaaccaggat gcttgattga
     6901 aatggcacgc atggatctat tcctcctttg cccattcttg gaatgctgta ttcaactctt
     6961 tgctaccttc agaaagtact agagtcgtac gagaaatatc cgtttcttcc actagagcgg
     7021 caagtacagc agcaactgta gagcgagtga tcgtttttga tgccttctca ttaggcttag
     7081 ttaattgaat ggaagtaact tcttcatcgt tagtcaatgt gactggttga agaatgacaa
     7141 aatctaagtt agttcgtttc agccattcat ctgcataatg tttagtaata taataatctg
     7201 tcatggactc aggccatttt gctggttcat ctgcataaac agcactgacc atcagatagc
     7261 gagagacatg cgcattttca gctgcgatca ctgttttgat tgcgccatcc aagtctactt
     7321 gcagaagatt ctttccttga gagccggcag caaaaatcaa tacatcgatg cctttgaatg
     7381 cttcggtcat tttttccacg gaccaagtta aatcaaaaga gacatacgaa acattctctt
     7441 tttttactaa tgtttgggaa gcaacatctc tcacccctgc aaaaatttca tgggaacttg
     7501 gagctaaatc ttttatcagg tgtcggccaa tttgtccgtt tgcacctaca acaaaaattt
     7561 tcatttggat catcctttcg acgtcaaatc tttccattca atagtaacac caaaagagaa
     7621 aaaaatcaga gatcggctac gatgactatc agttagtaag agaagggtgc ttgaagtatc
     7681 ttaacaagac attgcatact gctgcagtcg caagacctgc gacgattcct tcaggtatac
     7741 cacttgtaaa aataaccgct agaatagctg aataaacagc atttaaattt gttccgatga
     7801 ctttggcata atcttgctgg aataaaaagt agatcatatt catcactaaa atcgtattgg
     7861 tcaatgaacc ggaaagtcca gctaagaata aagaaacgga atctgctttt tgtttcatta
     7921 gtttgcgcag acctttataa acgaaataag gaaccacccc aatcaagatg cgaggaataa
     7981 acgcgatcag caaggctttc cagcttccat gttctgttcc gatgactggg ataaacggag
     8041 aaaagacaaa agacaaaggt gtttggataa ctgtgctacg gatcatgctg atcagtccaa
     8101 atgtaccgcc aagaaaagca cctagtcttg gtcctaaaac gatcgaagca atgattacag
     8161 gaatatgcat ggttgttgca ttgattggac cgatagggat aaaacctaaa ggcgtaacag
     8221 ctaataaaat caaaatagct aaaaacatcg cagttaatgt aaagtttttt gtatttttca
     8281 taagtactcc ttttttctaa gcatttcttt cattaagagt cagcatgacc tgttcgatga
     8341 tcgtctgaat atcagctaag gcaccttttc caaaatctcc acaagctaaa agagattctc
     8401 ttggttcaat ttcctgatag ccgacagatc tcaatgtttc cagattacgc tgagtgatcg
     8461 ggttttgata catattcgtg ttcatggcag gggcaatcaa ttttggtgtt cccactggaa
     8521 gcgccattgc gactgttgaa agaagatcgt ctgcaatgcc gtttgctagc ttgccgataa
     8581 tgtttgctgt agcaggcgcg atgagaaaca agtcagcttg ttttgctagt tcgatatgat
     8641 tgattacctg cggatttgat tcttgcatga cgtctgtatg gactgcgcgc ttcgacagag
     8701 attgcaaggt taatggcgtg ataaaatgtg tgctgctttt agacataatc acatctacct
     8761 gggcacctaa tttgacgaat tgacttgtaa gatcagcgct tttgtaagca gaaatacttc
     8821 cagtaacgcc tagtaagatc tttttcattt tgattcctcc agtctaattt tctcagtaat
     8881 caatgcagca atctcagatt tactttgagc ttcttccact gttccatctt tagataagag
     8941 atagccgtgg tgtgtctcgc catggatttc cgtcaaatca tttgcgaaaa taaaatcagt
     9001 tttgttattt atcagactat tttttgcgac tgccagaaga gattcctttg gtacatctac
     9061 taatagtttg aaaccaatga tttttgcttt tggctgccat tcacgtagat aagaaatgat
     9121 ttttggtgtt ttcttcaaaa cgagcacaag atgctcggtg ttagaagaga tctttgaaac
     9181 agtggtatct gtctgttcat tttccgcaaa ccaattgtcc agttcttctt gcgttgaaga
     9241 agtgggtagg ttttttgcca gctgttcttc tgaaaaacta aaagcaggag taaaatcact
     9301 gactgccata ctgtggataa tcgcatcgta gtgttctttt ttcgttagtt cttctaattg
     9361 taaaaataag tccagtgcag attcgatcgt ataaatcgtt atattggagt gtcttttcgg
     9421 tttcagtgct tgtttcgtag tcacgtaatc gattgtggta tccggattgg cagctaaata
     9481 atcagctaat gcttgcccta atcgtcctgt cgaatgattt gtaatagcac gtacttgatc
     9541 gattttttca gtcgttcctc cagctgtgat tagaatcttc atttatggtc acctacattt
     9601 actagtgttc ttccttggtg tgttccagca agtagtttag atgctgtttc aggcaattga
     9661 tccagagtaa tctcttggac gaccagttca tctgcaatct gcaaatccgc catctgttgc
     9721 cataaagaaa gacgttgatc tattggtaca ttgacagaat caattccaat caactggatg
     9781 tttcgcagaa taaaaggcaa gacagtcgcg tttagtttga ttcccccagc attgccacat
     9841 aaagctactg caccgttata agagatcaat ggaagaagac tgcttagctg ctcgccacct
     9901 actgtgtcga tgacatagtc gatttgctgt tttcctaaag cttttgttgt ctctggcaga
     9961 aattcatcag gtcgtacgac ttgcaaagca cctagacttt ttagccatgt gacagactct
    10021 tcttttctgg aaaaggtaat gattgactgg aatcctagtt tatgtaacag ggctatcgct
    10081 gtactgccta caccgccaga agcaccagta acgacaactt ttttatcgga ggtcatacct
    10141 tgacggatta aagcgttcac acacaacata gcagtaaacc ctgctgtacc aaggatcatc
    10201 gcttgacgca gcgtcatatt ttttggtaga ggaactaacc agtctcctgg tactttttga
    10261 tattggctat aaccgcctgg atgggtgacg ccaagcccaa aaccagtagc aagtacaaga
    10321 tcttcttttg aaaaacgtgg attgttactt tggacgacag ttccggataa atcaatccct
    10381 ggagtcatcg gatatgttcg tatcacctga ccagactcgc tcattgccaa tgcatcttta
    10441 taattgacat cagaataggc aatttttacc aagacttcat tttcagccaa ctcagtaaat
    10501 ggcagatcac tgattgctgt atggaaaaca ggttccttta ttacctgaaa agatcgaaaa
    10561 gcgttcattc gtgagacact ccttttaaaa ttttctttta tcataccata aagcctactc
    10621 ttttattctt gttcttccgt aaatttagac aaaa
//
LOCUS       DBKOIJ010000094        10647 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000094 DBKOIJ010000000
VERSION     DBKOIJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.096"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(190..1038)
                     /locus_tag="ACV5NJ_001857"
     CDS             complement(190..1038)
                     /locus_tag="ACV5NJ_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HHY6485940.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1202..2104
                     /gene="gnd"
                     /locus_tag="ACV5NJ_001858"
     CDS             1202..2104
                     /gene="gnd"
                     /locus_tag="ACV5NJ_001858"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HHY6485941.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2106..3107
                     /locus_tag="ACV5NJ_001859"
     CDS             2106..3107
                     /locus_tag="ACV5NJ_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HHY6485942.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3104..3388
                     /locus_tag="ACV5NJ_001860"
     CDS             3104..3388
                     /locus_tag="ACV5NJ_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HHY6485943.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3421..4218
                     /locus_tag="ACV5NJ_001861"
     CDS             3421..4218
                     /locus_tag="ACV5NJ_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HHY6485944.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4232..5065
                     /locus_tag="ACV5NJ_001862"
     CDS             4232..5065
                     /locus_tag="ACV5NJ_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HHY6485945.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5083..5940
                     /locus_tag="ACV5NJ_001863"
     CDS             5083..5940
                     /locus_tag="ACV5NJ_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHY6485946.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5962..6471
                     /locus_tag="ACV5NJ_001864"
     CDS             5962..6471
                     /locus_tag="ACV5NJ_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HHY6485947.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6508..6909
                     /locus_tag="ACV5NJ_001865"
     CDS             6508..6909
                     /locus_tag="ACV5NJ_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485948.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7021..7374
                     /locus_tag="ACV5NJ_001866"
     CDS             7021..7374
                     /locus_tag="ACV5NJ_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485949.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7408..7851
                     /locus_tag="ACV5NJ_001867"
     CDS             7408..7851
                     /locus_tag="ACV5NJ_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6485950.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7868..8953
                     /gene="uxuA"
                     /locus_tag="ACV5NJ_001868"
     CDS             7868..8953
                     /gene="uxuA"
                     /locus_tag="ACV5NJ_001868"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HHY6485951.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8946..9659
                     /locus_tag="ACV5NJ_001869"
     CDS             8946..9659
                     /locus_tag="ACV5NJ_001869"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6485952.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9866..10015)
                     /locus_tag="ACV5NJ_001870"
     CDS             complement(9866..10015)
                     /locus_tag="ACV5NJ_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485953.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10411..10611
                     /locus_tag="ACV5NJ_001871"
     CDS             10411..10611
                     /locus_tag="ACV5NJ_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HHY6485954.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3553 a         1687 c         2311 g         3096 t
ORIGIN      
        1 ttgttatcgc atctaacaag taactcgaat aataccaata caaccttctc caaagatgcg
       61 atgacatacg ttctaaccca ccgactattg atgttctgta ccacccttgc agaaccaaaa
      121 aagccactac caaatatttg gtagtggctt tttttgtttt atggattttt cgctttttct
      181 gttatggtgc tattctttga tttcagaaat agtatgtcga actttagcga tagaacgacg
      241 tgattctttt tcgttgtaaa acatcaaaat cacatataga gagtccaaaa tgcttagttg
      301 agaaatacgg gaagctaaag cttcagatcg gaattctgtt tcttcagaaa tagaaataaa
      361 aacgacatcg ccaagtttag caagggaaga attagcttgg ctagtaataa cgattgtttt
      421 tgctccagtt tttcttacgg tttcagcaat atggattgtt tcttttgatt gccctgtgtg
      481 agaaatcaaa acagcacagt ctttttccgt caataaagaa gcttccatca attgaatatg
      541 gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat aagcgtcagt
      601 tgctacaatc tctgaaccgc caacaccgaa aaaatagaca cgtttagatt cattgataat
      661 agtagccgct ttttttagat ccttttgttg taacaacttt tttgtgtcat ttaaagttga
      721 aatactagac tcgaacacct tttgcgccat agtgagctca gtatctgttt tctgaatatt
      781 ttcgtggata gaaatagcag atagatcatt ttcttggatc aacatggcca ttttaaaatc
      841 tttaaatcca ttataaccaa gtttttttgt aaattgaaaa aaagtagagt cagcaatgcc
      901 cagttcactt gctaaattgc taataggact atgacttact ttgcttggat tttctaaaat
      961 ataatcggcg atagcctgtt cttttgggct gaagtcgttg tacattgttt tgatttgtag
     1021 tcttatggag acgctcatga gaattcctca ttttcataat aagattatca ttttttccgt
     1081 tctcattaat gttaaagata caaggagagt aggaaaaaat caaacaaaaa atatttttgt
     1141 aaaatctatt tacaaaaaaa tttttttgta taatgagggt ataagaaatg gaggcgctat
     1201 catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa
     1261 agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag
     1321 agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa
     1381 aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact
     1441 ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga
     1501 ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac
     1561 ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga
     1621 aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta
     1681 tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg
     1741 aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga
     1801 cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga
     1861 aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc
     1921 tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc
     1981 agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc
     2041 aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga
     2101 gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc
     2161 cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag
     2221 agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg
     2281 ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc
     2341 gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg
     2401 tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt
     2461 tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg
     2521 aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg
     2581 caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc
     2641 tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag
     2701 aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag
     2761 agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat
     2821 ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg
     2881 gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc
     2941 cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat
     3001 gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc
     3061 ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt
     3121 gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc
     3181 aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac
     3241 ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt
     3301 tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat
     3361 catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta
     3421 gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt
     3481 aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg
     3541 aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca attagtgatt
     3601 gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa
     3661 tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa
     3721 gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat
     3781 atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt
     3841 gctggtttag ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg
     3901 catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta
     3961 gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta
     4021 tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg
     4081 aaagataaac agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat
     4141 ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt
     4201 tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt
     4261 gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt
     4321 gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt
     4381 tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc
     4441 aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt
     4501 attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg
     4561 aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa
     4621 agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt
     4681 tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt
     4741 gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt
     4801 aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga
     4861 ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat
     4921 tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca
     4981 aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga
     5041 agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat
     5101 aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga
     5161 tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta
     5221 tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca
     5281 tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact
     5341 ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc
     5401 ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga
     5461 atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct
     5521 tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat
     5581 caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca
     5641 ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg
     5701 tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg
     5761 atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta
     5821 cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca
     5881 caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa
     5941 aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg
     6001 atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt
     6061 gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc
     6121 cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa
     6181 tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac
     6241 ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat
     6301 cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa
     6361 ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct
     6421 gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct
     6481 tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa
     6541 tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt
     6601 tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat
     6661 acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt
     6721 atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg
     6781 aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt
     6841 ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta
     6901 tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa
     6961 gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg
     7021 atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa
     7081 ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt
     7141 cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt
     7201 tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat
     7261 atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga
     7321 gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa
     7381 agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag
     7441 ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa
     7501 acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa
     7561 ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt
     7621 ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact
     7681 tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt
     7741 cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact
     7801 gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc
     7861 tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa
     7921 atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc
     7981 tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg
     8041 actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga
     8101 tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg
     8161 ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt
     8221 taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat
     8281 ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg
     8341 ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac
     8401 cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga
     8461 aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt
     8521 tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc
     8581 atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt
     8641 tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt
     8701 tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat
     8761 gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca
     8821 tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat
     8881 gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga
     8941 gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg
     9001 gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta
     9061 aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca
     9121 ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag
     9181 aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat
     9241 cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag
     9301 tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg
     9361 atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga
     9421 atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa
     9481 ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata
     9541 atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg
     9601 aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat
     9661 aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg
     9721 aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac
     9781 aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga agaacgagaa
     9841 tcgtccatat gagggtttcc actccttatg cagatttttt taattcttgc actgcttgtt
     9901 cgatcgtaac acctgtcgct acatgattgg tattttcttt gtcaaaaacg acaaatagat
     9961 tcaaatctgt atccaaggtt actcgatatt gtatatcttt gttatagaat gtcatagcca
    10021 ttctcctttc tttaaatact tgctgaaagg caacgaacgc gttgataacg aaattccatt
    10081 tataagtata ccaccaattg aaaggttttc atagttaaga aattttaaag tcaaaagttg
    10141 cagataaaag caaataaaca agaaaacaaa aagtgaatca atcgcggtta ttcaactttt
    10201 tcttcgattc gaagacaata gaagacaatg aaatttatat ttcattgcat tatccttgtg
    10261 ttattagaaa aagcatgata tattaaatga gtaatctatt ttgaaacgta atttgagcga
    10321 tatatctaca tagataaaac tccttttgcc tatcaaatat ttattgcaac gaaaaaacgt
    10381 acattgatac gtatcaggag gaatttattt atgaataacg gtacagtaaa atggttcaac
    10441 gcagacaaag gttttggctt tatcactggt gaagatggaa atgatgtatt tgcacatttt
    10501 tcagctatcc aaggtgacgg cttcaaaact ttagaagaag gccaagcagt gtcttacgat
    10561 gttgaagatg gtcaacgtgg ccctcaagca acaaacattg ttaaagctta ataaaaattg
    10621 acttttaacc gtcttttcga aagaaaa
//
LOCUS       DBKOIJ010000095        10619 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000095 DBKOIJ010000000
VERSION     DBKOIJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.097"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            33..656
                     /locus_tag="ACV5NJ_001872"
     CDS             33..656
                     /locus_tag="ACV5NJ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485955.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACV5NJ_001873"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACV5NJ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HHY6485956.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACV5NJ_001874"
     CDS             2152..3033
                     /locus_tag="ACV5NJ_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6485957.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACV5NJ_001875"
     CDS             3036..3806
                     /locus_tag="ACV5NJ_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485958.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACV5NJ_001876"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACV5NJ_001876"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HHY6485959.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACV5NJ_001877"
     CDS             5117..6280
                     /locus_tag="ACV5NJ_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HHY6485960.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACV5NJ_001878"
     CDS             6397..6807
                     /locus_tag="ACV5NJ_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHY6485961.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACV5NJ_001879"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACV5NJ_001879"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HHY6485962.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACV5NJ_001880"
     CDS             9380..9664
                     /locus_tag="ACV5NJ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485963.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACV5NJ_001881"
     CDS             9824..10060
                     /locus_tag="ACV5NJ_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HHY6485964.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACV5NJ_001882"
     CDS             10057..10455
                     /locus_tag="ACV5NJ_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HHY6485965.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3713 a         1771 c         2243 g         2892 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtag
//
LOCUS       DBKOIJ010000096        10599 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000096 DBKOIJ010000000
VERSION     DBKOIJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.098"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..138)
                     /locus_tag="ACV5NJ_001883"
     CDS             complement(<1..138)
                     /locus_tag="ACV5NJ_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HHY6485966.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
     gene            294..416
                     /locus_tag="ACV5NJ_001884"
     CDS             294..416
                     /locus_tag="ACV5NJ_001884"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485967.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            389..520
                     /locus_tag="ACV5NJ_001885"
     CDS             389..520
                     /locus_tag="ACV5NJ_001885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485968.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
     gene            688..1647
                     /locus_tag="ACV5NJ_001886"
     CDS             688..1647
                     /locus_tag="ACV5NJ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HHY6485969.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            2124..2324
                     /locus_tag="ACV5NJ_001887"
                     /pseudo
     CDS             2124..2324
                     /locus_tag="ACV5NJ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3036..3641)
                     /locus_tag="ACV5NJ_001888"
     CDS             complement(3036..3641)
                     /locus_tag="ACV5NJ_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6485970.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            3823..4158
                     /locus_tag="ACV5NJ_001889"
                     /pseudo
     CDS             3823..4158
                     /locus_tag="ACV5NJ_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            4392..4550
                     /locus_tag="ACV5NJ_001890"
                     /pseudo
     CDS             4392..4550
                     /locus_tag="ACV5NJ_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236347.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4676..5647
                     /locus_tag="ACV5NJ_001891"
     CDS             4676..5647
                     /locus_tag="ACV5NJ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6485971.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            5787..7100
                     /locus_tag="ACV5NJ_001892"
     CDS             5787..7100
                     /locus_tag="ACV5NJ_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6485972.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            7143..8009
                     /locus_tag="ACV5NJ_001893"
     CDS             7143..8009
                     /locus_tag="ACV5NJ_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485973.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            8006..8854
                     /locus_tag="ACV5NJ_001894"
     CDS             8006..8854
                     /locus_tag="ACV5NJ_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6485974.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            8868..10340
                     /locus_tag="ACV5NJ_001895"
     CDS             8868..10340
                     /locus_tag="ACV5NJ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HHY6485975.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            10357..>10599
                     /locus_tag="ACV5NJ_001896"
     CDS             10357..>10599
                     /locus_tag="ACV5NJ_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HHY6485976.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         3393 a         1780 c         2131 g         3295 t
ORIGIN      
        1 cttcagttga tcagtatttc ttcggttttt aatcttatct ggtacacaat tgtggtatat
       61 gttagaaaca gttctcataa tttccttgaa tgttataaaa gtatctgtat aatgttcagt
      121 atattttgat gaacgcatat gtagtcctca tttcttggga gttttgagac tactatatgc
      181 gtttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      241 accacgggtt aaatctatat tttgatatac aaattacagc ttcagatact aagttgcata
      301 aaccttttcc ggaccccata caaaaggcaa ttgatgaatt gggcataaaa aataagaaac
      361 gatttatatt ggtgattcat tatatgacat gcaaagtgcg aaagcttgtg ggatagcatt
      421 tggtttagct aaatggggag ccaaaaatat agaaaatttt caaagtgtag atataattat
      481 tgattctcct caagaaatac ttgatttgtt agaaaaatag acaaagagcg tgagataagg
      541 atagggtctc acgctctttg tttttcgcca tttaaagatt gaaatttttg ccatcgccga
      601 ttgtaaaatt aagctagaca actaaaaaag tcatttgtgc tacactcaaa atagttccga
      661 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
      721 gtaataatag agtcttattt caaaattaat caatctgttg ctaaaactgc ccattgcttg
      781 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      841 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      901 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      961 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
     1021 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
     1081 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
     1141 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
     1201 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
     1261 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
     1321 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
     1381 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
     1441 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
     1501 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
     1561 attcctagaa aatcactgga ttatcgaaca cctttggaag tatttttgag ttacgtaagt
     1621 attgatgatc tgtctaactt aatttgacaa ttaagacatt taaaaagaaa agtgacagat
     1681 aggaataaaa acttattaaa tgtagttatg atctgttata tttcatttct aatactaggt
     1741 cttccacaag cagtgtttcc tgaaatgttt tctggagact ggggtattgt cagctattct
     1801 atattaacat tgattgctat ggttttaggt acttcatttt ataatgcttt tgtaagtaag
     1861 catgttgagg aatatgaagt attaatgtac ttaaaagaga aaaaaacaag taattaatat
     1921 gagaacacca cgcatagttt ttattttgag atattctaga atgaaacaca ttaagaacga
     1981 aagcaaagca aaagacgcca gaacttctac taccttctaa gttttttcat atttgtttgt
     2041 tatgctatac atagatctgc ataattagac ttgcgctgcc cgttattgaa gtttcaggca
     2101 gcgctctttt tgggggatcg ataatggaag aagaaaaaat atttgagaaa cgctggcagc
     2161 tagcatcaag tgaacaaaga gcaagataca ataatttgat cacctcctac cccactgttg
     2221 actagacata taaggaaaag aaatacttac tttggctgtg ccagttagat atagatacct
     2281 ttgaaacatt tgaagtgatc ctagataaaa ttaaacagaa ttgatcagaa acaagcgaag
     2341 ccttctctcg gttcgcttgt tttgctattt attagttggc tcagggcaac tctaagctcg
     2401 tttgaaagaa aaccgatgga taatcccctt gtttgtacca ctttagaata catggatgta
     2461 cgtattgcca ttgactccta ttagttactg aaaaaaaatt taaacaagtt gagtattgca
     2521 gataaatagc tccagtgagg tgaataactg ctccactcaa ggcaatagat agctccatcc
     2581 agggcaacaa actgctccac tttcatattt aatagatgaa taaccttctg tataatagaa
     2641 aggcatacct ttcggtatga caacttatac agaatgttat tttattggtt cactatcgaa
     2701 aaattttgga gctttatttt tccggtattt ctcaacgaac gattagttta actgtgggaa
     2761 gctccagaca tacgattcga gaagttactt ctcgagcaga caggaagggg gtgactgaac
     2821 taaccgctga tatgagcgat acttggctgt aggaatatct ttttccggaa aaacaaacaa
     2881 cagcacgagg ttaccttcct gtagactggg aatatgttca taaagaacta atgaaaaaga
     2941 atatgacatt gaaactcctt catagagaat atggggtaca tggaacatag aaacgaaaat
     3001 gcacggtaga aaagcgaatg aatatagtat aaattctaca aatcattcgc tttctttatt
     3061 ttatatatgg ttggtcgaga gagaccattt tctttggcaa tttctgcaac tggaactcct
     3121 tgatttaaca tgtccaccac agttttaaag actaatcgtt tttgcggatc tgttgagttc
     3181 tcagaatatt caattggtcg ccccttatat cgaccagctt ttttggccaa taatactcct
     3241 tgccgctgac gttcacggat tttctcacgt tcgttttgtg caatataact tagcaatgat
     3301 aaaaacatat cgaacatagc agtatctagg agttcatttc cagtattaaa atctaaaaac
     3361 tgtgcatcta gtatttttaa tgctactttt ttctgtttca taaaggtaac tgtatttttt
     3421 atgtcttcat aatctctgcc taggcgatcc agtgatgcaa caatgacaca atcaccttct
     3481 cggatataat ttagtaattt ttgaaactca ggtcgctgga tatctttacc actggctttt
     3541 tcttggaata atttatcaat gttaaggtct tccatcattg ccaattgtcg atctaaattt
     3601 tgatcttgtg aagacactct aatatatcca atttttgcca taaaaactcc ttttaaaaaa
     3661 tgatataatt tacatgtaaa atttacttgt agttttactg ttatcatagc atttttaaaa
     3721 ataaatttta agtctactta taatttactt tggaggtttt gaatgaatga aattaagcgc
     3781 aataaaaatt cttttggtgt ttatttagga gagcctagct cggaacagtt aaataaaggt
     3841 gaagctcgtc atgctctatg tcgaaatata ttttatggta gaaaaggcaa actttatcaa
     3901 acttattacg acggtatgga ggaacagctc aacgcattag gattagtaac taatgctatc
     3961 atatattgga attcactata tttagaaaaa gtctttgaac aaattaaaac ggaagggttc
     4021 gactgttccg aagaagtgtt acgcaagcaa tttccattaa ttagtgatca cattaatttt
     4081 gttggcaaat atagtttccg ctatgaaaaa gaattatcga atggaatact acgtccgtta
     4141 aatatagaga attattgaga atgttcgtct tttggttcct accgtgcatt ttcgcacgga
     4201 tgttccagat actatttttg aatgtataaa attgaaaaca caagaatact aataaatcaa
     4261 cgattcttgt gtttttaatt tttcactacc gtgcattttc gtttctatgt tccatgtacc
     4321 ccaatcttga aaaattaatt aaagacttag ataaagccat tgacgactta gtcgtggtgc
     4381 taccggagta tcaatgttta accagtatcc caggtattgg tcctgtttag gctgctggaa
     4441 tactcgctga aattggtcca attgaacgtt tttctgatga aataaaattg acaaagtatg
     4501 ccggcctata ttggcgacag agtcaatccg gtaaatctga atacgaaaat ttttttacga
     4561 aaagcttcac gtctaatatc tattttaaaa taaaaatatt cctgctaatt tggaatcgtt
     4621 tccttctttt ggtataataa tacctgaaat tcttttgaga ggagttgttc gtgaagtgaa
     4681 aactactatg aaggatgttg ctgcgttagc aggagttggt gtgggtacgg tatctagagt
     4741 aataaatgga gtaaaggtga aagaggttac caaagaaaaa gtaatggctg caattagtga
     4801 gcttaattat gagcctgatg aatatgccag aggtatgaaa acgaatagta gcaatacaat
     4861 agctcttatt ttacctacga tttggcatcc cttcttttcc gagtttgcct actatgtaga
     4921 ggaagctctc agccaaaaaa attataagct ttttttgtgt aatgcgaacg gcagtgcgga
     4981 aaaagaaaaa gaatatatcc aaatggtcaa gcagagtaaa gtagacggaa ttataggtat
     5041 tacctattca gacattgata aatatgtatc atcaaattta ccatttgtca gtatagaccg
     5101 ccatttttcg gaacaggttt cttatgtaac ggcagataat tatttaggtg ggcaaattgc
     5161 cgctcaagaa ctacttactc gtaactgtaa aaacttgtgc tatgttgggg gtgtttcacc
     5221 ttaccctaac gaaacaaaca atcgtaaagc tggtttttac aatatttgtg aggaaaataa
     5281 aatagctgtg aaggtattgg atatgcctga accaattagc aatttagagg aacaattgct
     5341 cgcttttttt tcggataatc ctgaaattga tagcattttt accatcaatg atttcatggc
     5401 tttggatgtg ataaaagccc aaaaaaaact agggaaggaa gcacctaagg attttcaaat
     5461 tattggtttt gatggagtca agctatcgtt gcaaaaagac tatttactat caacgattgt
     5521 acagccgata gaggaaatgg ccaaggtttc agtagaaaca ctcatggcga tgattttaaa
     5581 tgaagaaccg cctaagcgtg tggtagtacc ggttttcttt cacgctggag agacgactaa
     5641 agagtaaaaa accggtttat aacgattttt ttgagatagg ttgacagaaa gtgatttcaa
     5701 tgatatctta aaaccaatta attggaaacg ttaccaatta attgaaaaat aaaacggaaa
     5761 cgtttcaaaa aataggagga acatcgatga aaaagagaat gttagcaatt gcagcaattt
     5821 tagcattaac agctggagga cttgcaggat gtggcagtaa ggagaaaagt tcaggtagca
     5881 gtgatccagg agaaattaag atttgggttc agttttctga tgaaacgcct gaaggaaaag
     5941 cttgggcaga gattgtgaac aattttaatg atgaatacga gggtgaatat aaagctgtta
     6001 ctgagtatat tcctcgtagc ggaagcggcg gtggatatga ggataaagta aatgctgcaa
     6061 ttacaaccaa tacccttcct gatgttatta cgttagatgg accgaatact gcagcttatg
     6121 ctcattcagg agtcattgct ccattagatg attatttgaa agatgcggac atggatgatg
     6181 tattagacag cattattcag caaggaacct acgatggcaa aatgtatgcg tttggtttct
     6241 cagagtccag tgtgggtgtc tattacaata agtcgctgtt caaagatgct ggaattacag
     6301 aagatcaatt gccgacgtta gaagatcctt ggacttggac ggaatttcaa gcaatttgcc
     6361 aaaagctgac tgataaatac aaaacgcctg caattgatat gcaaatggcg tcgggagacg
     6421 aaatgctgac ctacgcatat acaccattta tatggtcgaa tggcggcgat gtcgttaatg
     6481 ctgatggaac caaagccgaa gactatttta acggtaaaaa atccgttgaa gcgttggaat
     6541 ttattcaaga attggttgaa aaaggttaca ccacaatcag tcctattgaa aaaggatttg
     6601 aaactggcaa atatgccatg atgctgagcg gttcttggac aatggcagat ttagaggaaa
     6661 actatcaaga gattgattat ggtattttgc cttaccccgt ttcagatgca actggggagt
     6721 tagtttcacc aaccggaagc tggcagttag caatgactga gaaaacagag aaaaaggatg
     6781 cttctgcggc ctttattaag tttgcaacca gtacagaatc tagtcgtatt atgagtttag
     6841 ggaacagtgt tctacctatc agaaattcaa cagttgattt aattaaagat gaagtgtcgg
     6901 aaggaatgca ggtattaatt aagcaaaacc aactttctgg acatgcccgc ccagtggttg
     6961 tagcttatcc gcaagtatct agagcattcc aacaagcagt tcaagatatt agctacttcg
     7021 aggaaaaccc agatgtacaa aaagtagcag atagtcgcgc tgctgaaatg caacaggcaa
     7081 ttgatacttc actaaaatag aagtttctaa agggggccaa tcaagtgtta aaaaaaccga
     7141 caatgaaaga caatttgata ggttacggct ttctagcgcc agctttgctg ctgctgacga
     7201 tttttctgat catacccgta atcatggtaa tttattatgc ctttactgat tattatttgt
     7261 taacgccaga tgctagacaa tttatcggct tagaaaattt taagcgactg ttcgctgatc
     7321 caatttttgt aaaaagtatt tggaacacgg ctaaatttgt tgtttggatt attccggtcc
     7381 aaataggggc agctttagga atggcactta ttatcaacaa aaatcgtaaa ggaaatatct
     7441 tttttaaagt agcctttttt gcgccagtcg tgatgtcctt agtcgttatt tcgattttat
     7501 ggttgtactt gttaaatcct aatgaaggtc tggttaatac gctatttgca aagattggaa
     7561 ttgcttcgca gccttttttg actagtccca aacaagcaat gtatacgctt gttttcgtat
     7621 cggcatggca aggtgcagga tatcagatgc tgctgttttt agggggaatg caaaatattc
     7681 cgcaagacgt ttatgaggca gccgaaattg atggtttttc taagtttcag caatttcgct
     7741 atatcaccat gccattatta aagcccacgg cgatttttat tttacttacc acattgattt
     7801 cggcctttaa actaatcatt cagccaatgg taatgactca aggtggacca atgaattcta
     7861 ctatgacaat ggtttattac atttaccaaa ctggatttac agaccgtatg gtaggctact
     7921 ccagctccat tgcgttaatc tttaccacta tgattggttt aattacaatt gctcaaagaa
     7981 aattggtaaa ggaggatgat ccagcatgac caagaaattt ggaccgctaa tgatcgtaga
     8041 atattgtttg ctgttccttt tagcggcatt attcattttc ccgatgctgt ggatggtagt
     8101 ttcctctatg aagcctgaag cagatgtcta tcaaaattta gcttccttca aagctttttt
     8161 acctagtttg aatccagcta attggtttaa aacttatcaa gaggttattt cccggttcag
     8221 tgtgggaact tatttattaa acagtatttt ttatggttta acctttgcct gtgggtcaat
     8281 cattgtaaac tctttggcgg gttttgcctt tgcgaaggtt aatttttcag gcaagaaaat
     8341 tatctttgga gctttgttag ctctgctgat tgtcccgatg gagacggttt taattccgca
     8401 atttaccatc gttaattcac taggcttagt gaatactcgc ttggcagtga ttttaccagc
     8461 gatggccagc gcgtttaata tctatctgtt tagaaatttc tttatcgcga ttccagaaga
     8521 aatcattgaa tcagcgaaat tagatggtgc aaatattgtt aaaattttct ttcacatcat
     8581 gctgccaatg tctaaaccag ccatagccac agtaggtgtc ttatccttta tctccagctg
     8641 gaatgattat atttggccgt taatggtctt aactgacagc agcaaatttt caatgcaagt
     8701 ggcaattact acgatcaata ctacacagcc agtttatatc aatcaagtga tggctgtttt
     8761 aaccattgca acgattccac tgattattgt ttatatcgtt gcacaaaaat atattctgca
     8821 gggacttggc ggttctggaa cgggtataaa ataggagtga atagtgaatg ttgcaaaaac
     8881 ttttaaaggc caatgagttt attgagaaaa atgctcaaac agtcaatcaa gaataccgga
     8941 ataaatttca tttgatggct ccgattggct ggatgaatga tccaaatggt tttgtttatt
     9001 atcaagagga gtatcacttg ttttatcaat attatcctta cgacagcatc tggggaccaa
     9061 tgcattgggg ccatgctaaa agcaaggatc taattcactg ggagcatttg ccagtagcac
     9121 tggcaccagg tgaaagctat gacaaggaag gctgtttttc cggcagtgcc atagaaaagg
     9181 atggcaagct gtatttgatt tatacaggac acaatgttgt ggaagggcaa gttcgacaag
     9241 ttcagtgttt ggcagtttca gaagatggca ttcactttga aaaatatgcc ggaaatccaa
     9301 tcatcgcaga agatcactta gcttctgttg caacgaccga agatttccgc gatcccaaag
     9361 tgtttcagcg aaaagaagac tattttactg tagtcgctac taaaactgca gatgctcgtg
     9421 gacaaattgt gatgttccat tccaaagatt tactaacttg ggagttttat tccgtattgc
     9481 tggagggaac tccagaacaa ggcattgtat gggaatgccc agacttgttt gaactagatg
     9541 gcaaagaagt cttgattctt tctcctattc aaatgactcc acaaggcaat gcttaccaaa
     9601 atacgaactc aactgttgct ttcatcggta ctgtcgattg gcaaacaggc aaatttctag
     9661 tagagaattt tcatgaaatc gatggcggtt tagattttta tgcaccccag acctgtgaaa
     9721 acgaagcagg ccaaagaata atgattgctt ggatgcaaat gtggcagcgt aaaataccta
     9781 cgaatgatct gggccatggt tggtcaggtt ccatgacctt gcctagagag ctgagggtgg
     9841 ctgataatca attgttgcaa cggccagttt catcaattta tcaagtaatt gatccgcagg
     9901 aaaggattga atgggcgaag actgaagaga atcgcttctt actgaaggac ttagtatatg
     9961 accagcacta tttacgcttg gaaatcaatt taaaacaggc aacgaaacta gaacttcact
    10021 atgcgaaggg acccgaccat acattactgc ttaactatga tgttgaacgg gagtgcttta
    10081 ctgcttccag ggaacacatg ccgtatgatt tatcaggagc tgaaaaggaa gtgttgaaaa
    10141 agcgcagtgt gacgattcct ttggaaaata attgtctaac catcgaaatt ttccgtgata
    10201 cttcagctat tgagcttttt ctaaatgatc gtctgactat gagtacaact ttttatgaga
    10261 cagaaaaagg acaggatttt cttttgctgg cagacagtga agtaaccttt tcattggaaa
    10321 gcggcttaat taatctatag aagggagagc tcagtgatga tttatggttc aatcgaagca
    10381 ggcggcacaa aatttgtgtg tgccattagc gatgaagaaa tgacgatcaa ggaaagagtg
    10441 agctttccga caacgacacc tgaggaaaca atacctttag tcattgactt cttcaaacaa
    10501 taccaagcag atttagcagg gattgggatt ggctcatttg ggcctatcga tatccatcgc
    10561 gattccgcca cttatgggta cattacctcg accccgaaa
//
LOCUS       DBKOIJ010000097        10575 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000097 DBKOIJ010000000
VERSION     DBKOIJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.099"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            12..84
                     /locus_tag="ACV5NJ_001897"
     tRNA            12..84
                     /locus_tag="ACV5NJ_001897"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACV5NJ_001898"
     tRNA            88..160
                     /locus_tag="ACV5NJ_001898"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACV5NJ_001899"
     tRNA            168..249
                     /locus_tag="ACV5NJ_001899"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACV5NJ_001900"
     tRNA            267..339
                     /locus_tag="ACV5NJ_001900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACV5NJ_001901"
     tRNA            351..422
                     /locus_tag="ACV5NJ_001901"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACV5NJ_001902"
     tRNA            439..524
                     /locus_tag="ACV5NJ_001902"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACV5NJ_001903"
     tRNA            535..608
                     /locus_tag="ACV5NJ_001903"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACV5NJ_001904"
     tRNA            618..691
                     /locus_tag="ACV5NJ_001904"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACV5NJ_001905"
     tRNA            711..783
                     /locus_tag="ACV5NJ_001905"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACV5NJ_001906"
     tRNA            811..884
                     /locus_tag="ACV5NJ_001906"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACV5NJ_001907"
     tRNA            898..971
                     /locus_tag="ACV5NJ_001907"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACV5NJ_001908"
     tRNA            995..1084
                     /locus_tag="ACV5NJ_001908"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACV5NJ_001909"
     tRNA            1097..1170
                     /locus_tag="ACV5NJ_001909"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACV5NJ_001910"
     tRNA            1189..1264
                     /locus_tag="ACV5NJ_001910"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACV5NJ_001911"
     tRNA            1266..1336
                     /locus_tag="ACV5NJ_001911"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACV5NJ_001912"
     tRNA            1350..1423
                     /locus_tag="ACV5NJ_001912"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACV5NJ_001913"
     tRNA            1448..1535
                     /locus_tag="ACV5NJ_001913"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACV5NJ_001914"
     tRNA            1557..1628
                     /locus_tag="ACV5NJ_001914"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACV5NJ_001915"
     tRNA            1651..1740
                     /locus_tag="ACV5NJ_001915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACV5NJ_001916"
     tRNA            1753..1826
                     /locus_tag="ACV5NJ_001916"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACV5NJ_001917"
     tRNA            1845..1917
                     /locus_tag="ACV5NJ_001917"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACV5NJ_001918"
     tRNA            1921..1993
                     /locus_tag="ACV5NJ_001918"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACV5NJ_001919"
     tRNA            1998..2068
                     /locus_tag="ACV5NJ_001919"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACV5NJ_001920"
     tRNA            2081..2154
                     /locus_tag="ACV5NJ_001920"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACV5NJ_001921"
     tRNA            2166..2237
                     /locus_tag="ACV5NJ_001921"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACV5NJ_001922"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACV5NJ_001922"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HHY6485977.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACV5NJ_001923"
     CDS             3826..5298
                     /locus_tag="ACV5NJ_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HHY6485978.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACV5NJ_001924"
     CDS             5381..6169
                     /locus_tag="ACV5NJ_001924"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHY6485979.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACV5NJ_001925"
     CDS             complement(6236..6904)
                     /locus_tag="ACV5NJ_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HHY6485980.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACV5NJ_001926"
     CDS             7019..8731
                     /locus_tag="ACV5NJ_001926"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485981.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACV5NJ_001927"
     CDS             8734..10506
                     /locus_tag="ACV5NJ_001927"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6485982.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3033 a         1926 c         2454 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcag
//
LOCUS       DBKOIJ010000098        10485 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000098 DBKOIJ010000000
VERSION     DBKOIJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.100"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            139..357
                     /locus_tag="ACV5NJ_001928"
     CDS             139..357
                     /locus_tag="ACV5NJ_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485983.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(646..1158)
                     /locus_tag="ACV5NJ_001929"
     CDS             complement(646..1158)
                     /locus_tag="ACV5NJ_001929"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HHY6485984.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1172..3469)
                     /gene="recJ"
                     /locus_tag="ACV5NJ_001930"
     CDS             complement(1172..3469)
                     /gene="recJ"
                     /locus_tag="ACV5NJ_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HHY6485985.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3611..4054)
                     /locus_tag="ACV5NJ_001931"
     CDS             complement(3611..4054)
                     /locus_tag="ACV5NJ_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HHY6485986.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4055..4843)
                     /locus_tag="ACV5NJ_001932"
     CDS             complement(4055..4843)
                     /locus_tag="ACV5NJ_001932"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HHY6485987.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4840..5781)
                     /gene="rnz"
                     /locus_tag="ACV5NJ_001933"
     CDS             complement(4840..5781)
                     /gene="rnz"
                     /locus_tag="ACV5NJ_001933"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HHY6485988.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5958..6545)
                     /locus_tag="ACV5NJ_001934"
     CDS             complement(5958..6545)
                     /locus_tag="ACV5NJ_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HHY6485989.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6766..8079)
                     /gene="obgE"
                     /locus_tag="ACV5NJ_001935"
     CDS             complement(6766..8079)
                     /gene="obgE"
                     /locus_tag="ACV5NJ_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HHY6485990.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8367..9377
                     /gene="gap"
                     /locus_tag="ACV5NJ_001936"
     CDS             8367..9377
                     /gene="gap"
                     /locus_tag="ACV5NJ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HHY6485991.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9462..10397)
                     /locus_tag="ACV5NJ_001937"
     CDS             complement(9462..10397)
                     /locus_tag="ACV5NJ_001937"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHY6485992.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2895 a         2369 c         1816 g         3405 t
ORIGIN      
        1 acgttggtct caaactcttt tttcgcccgg gttcttcttc tttcaaattt ttatcacttc
       61 cctctttaca aaccgaacaa atattcgtat actaaatata cgaacaattg ttcgcataaa
      121 ttaaagaggt gagaattcat gaaatcaatg agacgtcttg gcttagtatt aactgttttg
      181 tttttaggta ttattattgg taatttcggc atacgtccag ctttacttgt gtttgttccg
      241 ttatttactt tgtggttcat gttgtgggat gaaaagaagt accgtcaatc tgagcggcgc
      301 agaattcatg aagatcattt ctatcatgag ccctactaca gatccaggca atcataataa
      361 ggattgactg ctaattattc aaaagacaag aagttaaact tttacggggg tgtgacaaaa
      421 atcggtcaca tccttttttt cgaataaacg gtgttctaaa gccagttttt cggtattaag
      481 tcaaattgcg gaaaaagtga ttttttaaac ttctactggt ctttgcttgt ttccatttat
      541 aaaaaaatag cgaccctcaa aagtcagata gagaatctga cttttgaggg tcgctttaaa
      601 atcgcttctt taagaataac atcaagaata gcttcttttt tctgcttaat attccattaa
      661 tgtaatgatg tcatatcctt tgattttgtc gcgtccatgg agatccatca attcgatcaa
      721 aaatgcgcag cctacgacta caccgcctaa ttgttcgatc agttcgatcg tcgctttgat
      781 tgttcctcca gtagctagca aatcgtcaca aatcagcaca cgttgacctg gtttaatggc
      841 atccttatgc aatgtcaatg tatctgttcc atattctaat ccataagtca cttcgatggt
      901 ttctcgaggt aacttccctt ttttgcggac tggggcaaaa ccgactccta actcataagc
      961 aacaggacat cctacgatga atccacgggc ttctggccca actaccatat caattcgttt
     1021 ttcttttgca taatttacga tttgtttagt agcttcccga taagcgtcac catctgccat
     1081 cagtggggaa atatctcgaa acacaattcc ttttgacgga tagtcgggaa tactggcaat
     1141 ataatctttt aaatccactc tttgtttcct cctaggttgt taaccatttc tttatggtag
     1201 gaatatcgct caaaagcaaa aattcctcaa ctttgatttt attgagccgt cgctggtata
     1261 atctgctctc agtcaaagga tgactttctg ggctagcgac tttttgtaaa acaccgttct
     1321 ctattgtaac aaatttcaat tcaaaaaaca cctgtatcat aaaaattaat aatttttcag
     1381 ggatacgcaa atattgagct attgctttta ttttatgacg tatatcaatt cgttcttgag
     1441 aatggatcaa tttaaacaat tttgcgtact gttctcttgt gcctacccca ttcaaatagg
     1501 cctcatcagg tgaaatcccc atcagataaa cacgattgaa tgaataagac cgaaaaactt
     1561 ctttcagatc cgctaaatca tctggacaat ccataacaac tagagagttt ggttttcttt
     1621 tttcaattat tgcctttaca gaatctagtc cttcgaaaac aatcgcagct tcctggataa
     1681 tctcaggcac gaatttcaac gtcgaagaat caaatgctaa ataaaggcta ccttccgacg
     1741 ttcggctctg ttcacggaaa cgttttgccc gccaatcaaa tacttgcaga ccacccacag
     1801 aaaaatcaga taccatcagc tgcggtttct tacgcccgtt ccattcattg atcgaaagct
     1861 ttcctaccac atctacctga ttattcagca gttcttcttc ttgagtacca aaaccaaaag
     1921 cgaccgcatc aagctgactc gatgcatcgg aaaggacaaa tttcagatgc tgttggtttg
     1981 ctccgattct tttgatattt tctgcttgga cttgtcgaaa taaaaactga ggcaaaggat
     2041 tatccgtacc aaatggcgcc aatagtttca gctcatcaat tcgctctact gttacttcat
     2101 ttggcagcaa tacttcatca attctcaact caggccctct catcaagtca atttgatgat
     2161 cgactatata ttggttcatc ttctcctgta gtatagtgac attttcactt ggcatcgtta
     2221 aaccgactgc tgcatgatgt cctccgaagt aagtaaataa atctcgcatc tggtcaagca
     2281 tttcaaaaag attcaaggct tcaatactac gtccagatcc tttagctgat ccatcttctt
     2341 ttaaagtaag gacaagcgtt ggtttacccg tttcattcat tattttccca gcaacaatgc
     2401 ccaaaacgcc ttcatgccat cctcggttta ccaatagatg gatatgattc tccttattga
     2461 ccatcgctaa tgcttcttgt gttatctgct ctacgattgc ttttctttct tcattgatct
     2521 cattcaattt ttttgcttga acggatgctt cctcttcatc aaacgtcgca agtaacgaga
     2581 cggcaggacg tggatcgccc atccgaccga ttgcatttag tctgggagca atcgaaaaac
     2641 cgatcgttgt ttcgtctgcg tccctcattt ttacgccgct ttcttcaaat agcgcctgaa
     2701 gtcctatccg ttctgaatga tgaatggcat cgattcctaa tgaaacaagg attcgattct
     2761 catcagtcaa tgaaactaaa tcggcgatcg ttcctatcgc tactaaatcc aaaaattctg
     2821 caggcggttc ttccaataat gcagaagcta ctttgaatgc tactccgact cctgccaaat
     2881 ctggaaatgg atagtgcccc gctggatgac gagggtgaac aatcgcataa gcgtctggta
     2941 aattctcagg tagttcgtga tgatctgtaa tgatcacgtc tacaccagct tgctgagcgt
     3001 atgctaccgc atcatttcct gctaccccgt tatcaacagt gatgatcaac tggattcctt
     3061 cttcgatctt ttcttggtat accgattggt ttggtccata cccatgctca aatcgatttg
     3121 gcaggaatac ttctacttct gcaccaagta attccagtgt ttctttcatt accgttgcac
     3181 tggtgatccc atctgcgtca taatcaccgt aaacgaggat tttttctcca ctgattacag
     3241 cttcttggat tcggctgatt gctttatcca tatcgtgtat cagatacgga tcatgtaatt
     3301 cttgttcctt tggatgtaaa aatcggttga tatccttttc ctcatggtaa cctctttgcc
     3361 acagtaactt accgacaaat ggagataatt tcagttcacg gatcatttgg agaaaggatt
     3421 cagaaggctc tgtctcgctt aacttccaat caaaatttgc ttgtctcact ttctcactcc
     3481 taacttagtt attatagcaa aggaaatcag caaaaaaaag cagaaagaaa gaataaaaac
     3541 agatcagatc cgctagacag atgttcagca ggacctgatc ttttgtacga ccatttatga
     3601 aatatcccta ttagtcaaaa taattgaagc tttgtttggg cgattgagtt ttttgttctt
     3661 ccggcgcttt caattgactg atttgtgcgg ttttcgcttg taactccgcc tgataagcag
     3721 cttccaactc cgcacgttcg tttctaaact cgcgttgctg ttcttctttt gcttcttcaa
     3781 ttttcttttg aagttctgtt tgatattctg acatttcctg agtcagctgt ttgatttgct
     3841 ttttttgctg ccatagtgtt gccgaagcag tcaaaaatac aatcagtgca ccaatcagcg
     3901 cagaaccgat gatcactaag atcaatggcg cagaaaagga agtgaagcca aaacttacag
     3961 gaacattttt attgttcaat acagcaaaaa gaacaataat caaaactaaa acaaatccaa
     4021 gaatcacatt gctttgcttt ttcaattcct tcacctactt tttgttaaaa attccgctgg
     4081 ctaaataatc accaatcgtt ggaaacagcg tataaaattt tgatgcaact tctaaaataa
     4141 acggttgatt gatttcccgt tttggatgtc tcatattgcg gacgatcatt tttgccaact
     4201 tggcaggttc taaaacgatt tgtccgactt tttccaaata gcttccgctt ggatcagcct
     4261 tatcgaagaa atttgtctcg attggacctg gattcactgt cgtaacagct acgccaagtg
     4321 gtttcaattc taatcgcaaa gcgttggaga agccaaggac tgcaaatttc gtggcagaat
     4381 aaaccgttga ttttgcagtt gccatttttc cagccataga ggcgatatta atgatatgac
     4441 cttgtcttct ttctgccata tcgatcgcaa atttttgtgt caaaaccatc attcctaaga
     4501 tgttgacttc aaacatatcg tatgcttttc caaggtcgaa tgtaaggaag tcttcgaaaa
     4561 tcccaaaacc cgcattgttt accagaatat cgacgcgccc gacttcttcc atcacctttt
     4621 catataattc gtccacgctt tcaggattgc tgacgtctac cttgaaagca taagcctctt
     4681 tgccgctcaa gcgtctgcac tcgtctctga caccttctac aaatgccatt cttcgtgcgc
     4741 aacttataac gattgctcct tgctttgctg cttcataaca gatttgtgcg cccagaccgc
     4801 ctgaactacc tgttactaaa acaactttat cttctaaatt catgcctctt cctcctcgcg
     4861 aaatggaatc tcaatgctat caaaatcttt cataatcgtc gtgttttcaa acacttcttg
     4921 tgcttctttt tcaagctcta aagctgcttt tcctaaatat ctcgcactga tatgagtaag
     4981 tagcagccgt tttgcctgcg cttcttttgc aacttcagca gcttgatgag tcgtggaatg
     5041 gaaatagttg tgcgccattc ttgcttcatc tttattaaat gtgctttcat gtaccagaat
     5101 atcgctattt tccgcaagca caacggaatt atgtgttttt ctcgtatcgc ctaaaatagt
     5161 cacgattcgt ccttttttat ctggacctac gaaatctttt ccattgatcg tccgcccatc
     5221 ttctagttgg atcgtttcac cttgcttcag tttgccgtac agtggtccag ctggaatacc
     5281 taattctttt agacgatcca cctgcaattc tcctttatga tcatgttcaa ccacacgata
     5341 accaaaactg tcgatcccat gatttagttt ttttgtataa acagaaaatt gctgatcagt
     5401 aaaaatcggg gctgtttcat ttagctcaat gaatttcagc ggataactta aacgcgtttg
     5461 cgaaatacct aaagagactt ttatgtattc ctctatccct tttggcccgt aaatctctaa
     5521 tggcgtatcc ccaccttgaa atgaccggct gctgatgagc ccgggaagtc caaaaatatg
     5581 atcgccgtgt aaatgtgtaa tgaaaatttt accgattttt ctcggacgaa tagtcgttcg
     5641 caaaatctgc atttgcgtgc cttcgccaca gtcgaacaac caaacttcat tgcgctcgtc
     5701 taaaagtttc aatgctatac ttgtcacatt tctatgtttt gcaggaacac ctgcacctgt
     5761 tcctaaaaat tgtaattcca taatctacct gactttcttt aagttcctct ttaattttag
     5821 ggtaaaaaga agggataatc aaccgtaagt cgagaatggg ttcaaattaa aaataacgtt
     5881 ctaaagcgat agcgttttct ttgaaaatta ctataaaaac agcagcgaac ccgtttgaga
     5941 tcctctgctg ttttttatta atttgaagat ccagaacttt cattgtcgtt ggaagtttgc
     6001 ggatgtttgt ccttccaagt cttttgtgca gcttctttcg catgattttc agcttctttc
     6061 gcatcatgca catctttttc gacatcttca taatccaagc gagtaatttc tccgcctaat
     6121 tccatcatta ccaattgatt cagtgggcga ttgtcctctt catcagcaat caagatcagt
     6181 cctgtagctc cttcaccgat tttatttcct acaaagtcga acacggtctg ggcatctttt
     6241 atttctttgg catcttgtgt cgcaccgatc attcctccgg caaaccatcc taataaaatc
     6301 cctagcggac cacctaatac accaaccaac attccgatta atccacccgt agaagatttg
     6361 ttattaccgg taaagtcgat aaaatcttct attttgaatt gatgtgtccc attaggatca
     6421 tggctgacaa ctgccatctg ttctccttta atcgaacgaa cagcatgtaa ctttttgatt
     6481 tctgaaaatg cctgatatgc ctggctttct acttgaaaat ttaaaatgat tactcgtttg
     6541 ctcattaaaa acgccccttt cataccttct tgttatttat tttaccttta attgaaaaat
     6601 aagaaaagaa aagcactctt tttttccaat aaaatagggc tgtgacaaaa gtcgtatcac
     6661 agctcctttt tccgaataaa cggcgttctt gaagctgatc acttctgtca caacctccct
     6721 taaatctgaa tctatatttt ttggtatttg tttttatttg cttgcctatt cgacaaattc
     6781 aaattcaaac tcaccgatac gtacaaggtc gccatctttc gcaccgcgtg cccgaagagc
     6841 ttcatccaca cccataccac gtaattgacg agcaaaacgc atcactgttt catcatgatc
     6901 gaaattcgtc atttggaata atttttcgat cttctcacca gataagatcc acgttgcatc
     6961 tgaatcacga tcgatttgga actcaggacc ttctggattg aatccatagt gtacagtttc
     7021 ttcttccagt tcttcttcgt acaatgggaa ttctggcgtt acttctaaca aatctgctgt
     7081 agcattcaat aacgcgtcca atccttgtcg agtcactcca gaaattggaa atactgggat
     7141 gtcatcagca aattcatctt ctttttcctt gttcagctgt tctttgaatt tcactagatt
     7201 ttccggtgca tccggcatat ccattttgtt tgccacgatg atttgcggac gctctagcaa
     7261 gcgtaagtta taagtagata attctttgtt gatggcaaga tagtcttcat aaggatctcg
     7321 tccttccatc ccactcatat cgatcacatg taaaattact cgtgttcttt cgatatgacg
     7381 aaggaattgt gtacccagcc caacaccttg cgaagctcct tcaatcaagc ctggcaagtc
     7441 tgctgcggca aaacttctgc catcacttgt agtaaccatt cctagattag gtacgagcgt
     7501 cgtgaaatga taagcgccga tttttggacg agcagatgaa ataacggata gtaaagtcga
     7561 tttcccgaca gatggaaaac ctacaagtcc aacgtctgcc aatactttca attctagttc
     7621 gatttttcgc tcttgtcctg gttcaccgtt ttcagcaatt tccggtgcag gattccgagg
     7681 agaggcaaaa cggatattcc ctcgtccacc gcgtccacct tttgccacaa ctagtgtttg
     7741 accattttca attaaatctc ctaaaagagc accagtctcc gcatctcgga cagtggttcc
     7801 ttgtggtact ttaacatagg tatcttctga accacgaccg tgcatccctt tactcatccc
     7861 attttcacct ggctgcgctt tgaaatggcg attgaaccga aaatccatca atgtacgtaa
     7921 cccttcatcg acgatcaaga tgacatcgcc gccacgacca ccgtctccgc cagccggtcc
     7981 gccgtcagga acgtattttt cgcgacgaaa tgcaaccatt ccgtctccgc ccttgccagc
     8041 tttcacatcg atcgttactt gatctaaaaa catggacata attaagtcct cctattattc
     8101 atttatttat ttgactgcct gaatttccac aataaacaaa agtcttactt attttatcgg
     8161 tgaaatctga ttttgtctat catttcagca aaaagattgt ttaccttctt ctttttttat
     8221 catttggtaa ggtttccaac cttttttctg ctaccaagta taattttttg acaaaatatt
     8281 ctttgcgtag cagaataaca gtgtattaat tgtattaatt gaggatgccg tttcacagca
     8341 cccctaataa aggaggaact tttgatatga cagtaaaagt aggtattaac ggatttgggc
     8401 gtattggccg tctggctttc cgccgcatca aagaagtctc agatgatatt gaagtagtag
     8461 caatcaacga cttaacaagt ccaactatgt tagcacaatt attgcaattt gactctactc
     8521 atggtactta tcctggcaca gttactgcaa cagaagactc aatcgtagtg gatggcgaag
     8581 caactcgtgt ttatgcagaa cccgacgcaa gcaaaatccc ttgggcaaaa gaaaacggtg
     8641 tagatatcgt actagaatgt actggttttt atacatctga agaaaaagca aaagcgcatt
     8701 tagatgctgg tgtcaaacga gtagttattt ctgccccagc tggtgctatg aaaaccatcg
     8761 tctataacgt caatgatgat acattagatg aaaacgacaa aatcatctca gcaggttcat
     8821 gtacaacaaa ctgtttagca cctatggctt atttcttaaa taatgaattt gggatcgaag
     8881 ttggtacaat gacaacagtt catgcctaca cttccacaca aatgctctta gatggacctg
     8941 taaaaggcgg taatttgcgt gctgctcgtt cagctgctga caatactatc ccacattcta
     9001 ctggtgctgc gaaagcaatc ggcttagtta tccctgagct acaaggaaaa ttacaaggac
     9061 acgcacaacg tgttccagtc gttgatggtt cattaactga attggtttca gtattgaaaa
     9121 caaaagtaac tgctgatcaa gtaaacgaag caatcaaaaa acacacagtt gacaatcctt
     9181 cattcgggta tgatgaccgt caaatcgttt ctagcgatgt cattggaaca actcaaggtt
     9241 cgatctttga cccaacgcaa acagaagtaa caactgctgg tgatttccaa cttgttaaaa
     9301 ctgttgcatg gtatgacaat gagtatggat ttacttgcca aatgattcgc ttactagaaa
     9361 aattcgctaa cttgtaattt gatagatcaa tcgataaaag aagcctttga ctggatccag
     9421 tcaaaggctt ctttcttttc atcccctact tttaaactcg ttcaatgaac atattggctt
     9481 aaaaattgtt ggactacttg ctgatatttt tcaggatctg tcttgtatgc ttccgcatgc
     9541 tcggctcctt ttacaacata cttttctttt ggtcccttcg ttgcacgata gaccttatct
     9601 aacatttcaa atggtacaaa tgtatcttcg tctccatgta taaagagcat tgggcgctgg
     9661 tttttcttca attgttccac tgcatttgct tcaccgaaaa agtagccagc acggactttt
     9721 gtcactagac tcgttacttg cataagcgga aatgctggaa gactaaacat atcgtctagc
     9781 tgataagcaa gctcatcatg aacggaagaa taaccgcaat cttcaatgat tgctttcaca
     9841 ttatctggta gtttttctcc gctagtcatc ataactgttg ctgctcccat gctgacaccg
     9901 tatagaacga tttcttgcga ttttccattg ttttctagca ctttgtttat ccactgtaca
     9961 tagtctttac gctctggcca tccaaatcca atgtaatccc cttcgctttt tccatgtcct
    10021 cttgcatcag gaacaaggac attgtaaccc atatcatgaa acatctttgc gtaattggtc
    10081 atcgtttcag cgtttcccat atagccatga gccacaagag ctgttttttc acttttcgtt
    10141 tcagcaggaa gataaatcgc tgataatttc agaccgtctt ttgaagtgat ggtccaatcg
    10201 cttcggtttt cttttgaact aaaccattta tgtgcatccg tttctttgga tgtctgtgtt
    10261 gtaccaggtg tatcttcgtc taaaaagtct ttctcagaag gcacaacagc ataattataa
    10321 aaataattcc ctgcaaacac taacgcaaca atcagagcta aagcgacgcc taaaaggatt
    10381 ttcatccaaa gtttcataaa tcatccatct catttctctt ttttcatata accttgaatt
    10441 agtataatca aagaatctct actatgcaat gttcattttt ataac
//
LOCUS       DBKOIJ010000099        10367 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000099 DBKOIJ010000000
VERSION     DBKOIJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.101"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            6..305
                     /locus_tag="ACV5NJ_001938"
     CDS             6..305
                     /locus_tag="ACV5NJ_001938"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6485993.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACV5NJ_001939"
     CDS             378..1739
                     /locus_tag="ACV5NJ_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HHY6485994.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACV5NJ_001940"
     CDS             1761..2021
                     /locus_tag="ACV5NJ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6485995.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACV5NJ_001941"
     CDS             2035..2787
                     /locus_tag="ACV5NJ_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HHY6485996.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACV5NJ_001942"
     CDS             complement(2881..3486)
                     /locus_tag="ACV5NJ_001942"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHY6485997.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACV5NJ_001943"
     CDS             complement(3539..4513)
                     /locus_tag="ACV5NJ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HHY6485998.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACV5NJ_001944"
     CDS             complement(4891..5610)
                     /locus_tag="ACV5NJ_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6485999.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACV5NJ_001945"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACV5NJ_001945"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HHY6486000.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACV5NJ_001946"
     CDS             7161..8558
                     /locus_tag="ACV5NJ_001946"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HHY6486001.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACV5NJ_001947"
     CDS             8639..9079
                     /locus_tag="ACV5NJ_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HHY6486002.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACV5NJ_001948"
     CDS             9227..9553
                     /locus_tag="ACV5NJ_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HHY6486003.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACV5NJ_001949"
     CDS             9554..10303
                     /locus_tag="ACV5NJ_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HHY6486004.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3288 a         1829 c         2149 g         3101 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgctt
//
LOCUS       DBKOIJ010000100        10265 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000100 DBKOIJ010000000
VERSION     DBKOIJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.102"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(59..1567)
                     /locus_tag="ACV5NJ_001950"
     CDS             complement(59..1567)
                     /locus_tag="ACV5NJ_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HHY6486005.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1726..1917
                     /locus_tag="ACV5NJ_001951"
     CDS             1726..1917
                     /locus_tag="ACV5NJ_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486006.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2120..3037
                     /locus_tag="ACV5NJ_001952"
     CDS             2120..3037
                     /locus_tag="ACV5NJ_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HHY6486007.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3030..4103
                     /locus_tag="ACV5NJ_001953"
     CDS             3030..4103
                     /locus_tag="ACV5NJ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486008.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4251..4769
                     /locus_tag="ACV5NJ_001954"
     CDS             4251..4769
                     /locus_tag="ACV5NJ_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486009.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4750..5142
                     /locus_tag="ACV5NJ_001955"
     CDS             4750..5142
                     /locus_tag="ACV5NJ_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HHY6486010.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5218..6153
                     /locus_tag="ACV5NJ_001956"
     CDS             5218..6153
                     /locus_tag="ACV5NJ_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HHY6486011.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6286..7065
                     /locus_tag="ACV5NJ_001957"
     CDS             6286..7065
                     /locus_tag="ACV5NJ_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HHY6486012.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7058..7375
                     /locus_tag="ACV5NJ_001958"
     CDS             7058..7375
                     /locus_tag="ACV5NJ_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HHY6486013.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7619..9229
                     /locus_tag="ACV5NJ_001959"
     CDS             7619..9229
                     /locus_tag="ACV5NJ_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HHY6486014.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9386..10234
                     /locus_tag="ACV5NJ_001960"
     CDS             9386..10234
                     /locus_tag="ACV5NJ_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HHY6486015.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3229 a         1800 c         2071 g         3165 t
ORIGIN      
        1 aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta aactttttct
       61 attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg attcctagtt
      121 ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct tgtgctccct
      181 caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact gcactgattg
      241 ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga agtgtctttg
      301 atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg tcatctgcga
      361 tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc atcaatgaac
      421 ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca gcagaaggat
      481 cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg actaccacgt
      541 ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga ttaactgctt
      601 cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga cggatgattt
      661 cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct acacgattca
      721 cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt tcaataatac
      781 gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact ttattgtagg
      841 catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg gctgcttcat
      901 aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt tcgaacttgg
      961 agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag caagtagcga
     1021 gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt gtttcaatat
     1081 actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact ggatagtctt
     1141 cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg ataaagactt
     1201 tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat aagccataac
     1261 cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca taatcaccac
     1321 ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca gcttgatctt
     1381 tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt acacgtttcg
     1441 catgcttgtc gtcaactaac ttcccaccaa attctaaata taatttgtca tagtggttca
     1501 ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca aaaccaattt
     1561 ttttcactag gttccctcct taagtactaa agcatttttt catttttttg aggattcgtc
     1621 aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag
     1681 atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa aaaaagacag
     1741 aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta tgaaagaatg
     1801 cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga aacgcagcta
     1861 actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag taaataaaaa
     1921 ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact agttgatcga
     1981 aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc cgctcttttt
     2041 tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa aaacagatga
     2101 gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg agccagacga
     2161 actagaggag acgaacgcgg atcaggatac aggacaagaa tcgtcttctt tttacgatat
     2221 caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg cttttgaaaa
     2281 agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc gattgaatga
     2341 agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga tgcgtaagat
     2401 ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca tctcgccaac
     2461 gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg gtattgatgc
     2521 aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga aatattccca
     2581 accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg tgatcactgc
     2641 tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac aaatgactga
     2701 tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt
     2761 agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt atattgagac
     2821 gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag cagaccagct
     2881 tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga
     2941 gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg agaatcctaa
     3001 taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca gaatggacaa
     3061 aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa gaacaaacaa
     3121 gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa atacatgaaa
     3181 aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct tacgaaataa
     3241 ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc gtcagtttga
     3301 ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg atcgaatcag
     3361 attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa ccaatctcca
     3421 cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa ccgttgaatg
     3481 atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga catttttcga
     3541 tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta ccgaccatcg
     3601 atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag acaatggaaa
     3661 tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg gatcgagtat
     3721 tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta gcccatgcaa
     3781 gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca cttcgtttga
     3841 atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt atcgtggcaa
     3901 tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa gtacgattag
     3961 ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct
     4021 catctggaga ctacccacga tctgtcattg atttctttat caattacgaa ttcttggtcc
     4081 cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca ttcagataat
     4141 tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct tttctgtatg
     4201 tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga atgtccacaa
     4261 ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc
     4321 aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta ctttacttaa
     4381 gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt ttttattttg
     4441 gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg agtttgtttt
     4501 caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga gtctgttttt
     4561 ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg ttatatcgtt
     4621 tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg cacagtatag
     4681 gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac aatcattcag
     4741 gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag tatactcatt
     4801 ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga ggaagtaaaa
     4861 acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa
     4921 gtggtcttca aaaaaggcga tgatttactg agtattttga aacgaaattt tgaagtcaaa
     4981 gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa aaatcaactc
     5041 tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga gatcaaattg
     5101 aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata aaaaactgtt
     5161 aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga aaataacatg
     5221 gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc taatattcta
     5281 gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat gctaaacgaa
     5341 agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg tgataaacaa
     5401 gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa atactttttc
     5461 agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt gatggaagct
     5521 tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt tatagcaata
     5581 ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa
     5641 gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa
     5701 atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt ggctttagca
     5761 ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag cggattgctg
     5821 atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta tagtctgatc
     5881 atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt tgaacgtcct
     5941 gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga gattttagtt
     6001 gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt catcaatgat
     6061 attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata cattgaaaca
     6121 gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa
     6181 atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga atttatgtta
     6241 caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa
     6301 agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag ctatatacca
     6361 gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct cttatcagga
     6421 ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc tatgcttacg
     6481 acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt acgctataca
     6541 ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc
     6601 ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac
     6661 ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc
     6721 ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc
     6781 cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta
     6841 ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa
     6901 aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag
     6961 aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag
     7021 ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact
     7081 tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt
     7141 tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt
     7201 atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg
     7261 gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat
     7321 ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt
     7381 tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg
     7441 catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt
     7501 ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga
     7561 ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat
     7621 gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt
     7681 tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat
     7741 gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg
     7801 tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga
     7861 taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat
     7921 cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc
     7981 taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc
     8041 aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac
     8101 ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt
     8161 gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt
     8221 cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt
     8281 cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc
     8341 tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt
     8401 tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc
     8461 agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt
     8521 gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat
     8581 gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt
     8641 attgattctt cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt
     8701 gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt
     8761 gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt
     8821 tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt
     8881 aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt
     8941 tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag
     9001 caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt
     9061 tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg
     9121 tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg
     9181 gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc
     9241 gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt
     9301 ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat
     9361 tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata
     9421 tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat
     9481 ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt
     9541 aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt
     9601 atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg
     9661 gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt
     9721 cattccttat gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt
     9781 agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg
     9841 agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg
     9901 tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt
     9961 ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg
    10021 agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt
    10081 gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata
    10141 tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga
    10201 aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa
    10261 aaata
//
LOCUS       DBKOIJ010000101        10212 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000101 DBKOIJ010000000
VERSION     DBKOIJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.103"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            113..2809
                     /locus_tag="ACV5NJ_001961"
     CDS             113..2809
                     /locus_tag="ACV5NJ_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HHY6486016.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2874..3374
                     /locus_tag="ACV5NJ_001962"
     CDS             2874..3374
                     /locus_tag="ACV5NJ_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6486017.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            3386..3880
                     /locus_tag="ACV5NJ_001963"
     CDS             3386..3880
                     /locus_tag="ACV5NJ_001963"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6486018.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3901..4710
                     /locus_tag="ACV5NJ_001964"
     CDS             3901..4710
                     /locus_tag="ACV5NJ_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHY6486019.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4707..5528
                     /locus_tag="ACV5NJ_001965"
     CDS             4707..5528
                     /locus_tag="ACV5NJ_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHY6486020.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5579..7678
                     /locus_tag="ACV5NJ_001966"
     CDS             5579..7678
                     /locus_tag="ACV5NJ_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HHY6486021.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7675..8793
                     /locus_tag="ACV5NJ_001967"
     CDS             7675..8793
                     /locus_tag="ACV5NJ_001967"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HHY6486022.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8832..9710
                     /locus_tag="ACV5NJ_001968"
     CDS             8832..9710
                     /locus_tag="ACV5NJ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HHY6486023.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9856..10053
                     /locus_tag="ACV5NJ_001969"
     CDS             9856..10053
                     /locus_tag="ACV5NJ_001969"
                     /inference="COORDINATES: protein motif:HMM:NF016526.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HHY6486024.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         3148 a         1671 c         2282 g         3111 t
ORIGIN      
        1 gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta gataaattat
       61 tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa aattggaata
      121 caaatataga atagacatat tgcagccgat agtttattat gcttttagac acattaaact
      181 ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga tacgactaac
      241 agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta tctctcgtca
      301 gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga aattaccggg
      361 aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata cggagcaaat
      421 aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg tcattcaaaa
      481 atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa atggagccac
      541 gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc atgagcaaat
      601 tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg acaatcccga
      661 actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg ctgaaaagga
      721 taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg aggtccatcg
      781 cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa actatcgtcc
      841 agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg ccacgacgga
      901 aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta gtgttatgtt
      961 gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat tattttttca
     1021 aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct tgtctttaat
     1081 gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta aaatcagttg
     1141 tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa atgagatctt
     1201 gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta gtttattgat
     1261 aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg attcgatttt
     1321 ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt cttcgataaa
     1381 gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat caatgagtag
     1441 aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa aaacgtacgg
     1501 attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca tttccccaag
     1561 ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac ctcggacgac
     1621 ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg acgagaactt
     1681 tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca tcgagctgaa
     1741 gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag tagtgaatca
     1801 attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta atgtgtcaga
     1861 gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata gtctagtgct
     1921 gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg atttaaaagg
     1981 ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg gcttgaagat
     2041 gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt attcaatcaa
     2101 tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct gtatgtcggg
     2161 tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg atgaatccat
     2221 cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa atgatgaaac
     2281 ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt ccttttcaat
     2341 tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt tggagcatat
     2401 tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc tagtaaaatt
     2461 cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga tcgtgatgag
     2521 tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt taaatggccg
     2581 tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat tcttggctcg
     2641 gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg aattttatta
     2701 tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag tcaatgctta
     2761 tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa gtgtctaaaa
     2821 aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt aacgtgatta
     2881 aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac agaaagaaag
     2941 gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct tcaggctttc
     3001 ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt aatgcttatg
     3061 tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg aacgagcagg
     3121 atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag attaccaaaa
     3181 cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg ccaattctgc
     3241 tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa ctggtaaata
     3301 aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta aatgaagagg
     3361 aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg aacgattaat
     3421 tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt tagtagtagc
     3481 aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg ctgcaccatc
     3541 gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa aagatccgcg
     3601 ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg taaaagttgc
     3661 gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga aaatgacacc
     3721 gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt cagaaattca
     3781 agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta caccggcgat
     3841 ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag gagaaaaatt
     3901 atgtatgatg catttgtagt cgctttggcc gtttttattg gagttgctgg acatgaaatg
     3961 ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg agcgttgatg
     4021 ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt tatgggtgtc
     4081 gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct ggctacagcg
     4141 ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt tccactagca
     4201 gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc gatggctgct
     4261 aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct gcacttcttt
     4321 atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt tttatttgga
     4381 gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa cggattaaca
     4441 gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat gctttggagt
     4501 aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct cgagttacca
     4561 ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca aagagatcta
     4621 caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa tgcaacgctt
     4681 gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct aaagatgaga
     4741 aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg gtgaatccgg
     4801 tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat catttctaca
     4861 aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt tcgacgacca
     4921 ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa aattctgaaa
     4981 aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg ggaccattgg
     5041 ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg ggactagcga
     5101 tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt atgttcaata
     5161 tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta ggctctaaat
     5221 atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct gctagtatct
     5281 tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat accgttttga
     5341 gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat agtattttta
     5401 aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat aaaaaagtcg
     5461 gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca ttacttggaa
     5521 tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga gggaaaagat
     5581 gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg agttaaacga
     5641 cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg atgtaaaggg
     5701 attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt gcgggacgat
     5761 tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag gtgtttgggt
     5821 caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa acgggattgt
     5881 ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag actgtttgcc
     5941 tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg ctcccatact
     6001 ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg aaacacacga
     6061 aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt cggatcagca
     6121 aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg aagtggcaag
     6181 cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt ttcaggaatc
     6241 tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat ttgggcagct
     6301 gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc aaagcttttc
     6361 tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca gctatcctgc
     6421 tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca ccatgtttga
     6481 accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta agtttcctgg
     6541 ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca gtgcgttact
     6601 ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt gtccgcaggt
     6661 tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt caggggaacc
     6721 aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt atcataccgg
     6781 cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg aagccctaga
     6841 acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg aatcaatttg
     6901 taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa tcggtgatga
     6961 ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc aagggttaat
     7021 ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg atggagaaga
     7081 atcagacact acgttgatgc ctttttatca atttttagat aataaagatc atttatatca
     7141 gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg aaatcaaagg
     7201 catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg ttttaatgag
     7261 cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt gtttggcaga
     7321 tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag gagaagtcgt
     7381 gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca gcctaatgat
     7441 cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc ctgttgataa
     7501 tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga ttgaatgtac
     7561 gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa gtgatacgaa
     7621 aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa aaagatgagt
     7681 atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac tcccgaaaat
     7741 ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc aagccgcaaa
     7801 ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga tattactgga
     7861 acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt tggcagtttt
     7921 gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc tgctgttgaa
     7981 agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt gaactcgttg
     8041 ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc tttccaaaca
     8101 tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt tttttatacg
     8161 ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca cgcaaattgg
     8221 catcgggaac gcattaccaa attacaggag gattataagt taattgatcg attaaagggt
     8281 caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta ttggtggggc
     8341 gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt aacctcaaca
     8401 ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga gttcggacgt
     8461 ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc aaatagagat
     8521 gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt tggtgacgac
     8581 gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg ttttaccgaa
     8641 gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt tgagcggcaa
     8701 gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa tactaaacgt
     8761 catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat attcgaaggg
     8821 aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt gtctgtgcag
     8881 tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca actccagttg
     8941 aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg gcaggcatcg
     9001 gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc ggtcatatca
     9061 catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta aagcaatatt
     9121 tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt gaatatgttg
     9181 ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca ggtatcggag
     9241 gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa atggggcata
     9301 tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc caccaagatt
     9361 gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta aaaggccaga
     9421 atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc caatgtgcat
     9481 acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt gtaatgaaac
     9541 aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat ggatatgttg
     9601 aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat gccggaataa
     9661 tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa ttaatgaatg
     9721 tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca aatgaagttg
     9781 gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt gttttcatta
     9841 aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc gctgtatacc
     9901 ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta ctttatgtct
     9961 gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt tgacttcata
    10021 atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt ataaaataaa
    10081 tcgtaattct attatagaga gttgtgatta taaaaagata gcaagaaaaa aaaagaaagt
    10141 ttctatacat tttaaatatt ttattcctat ttctattgct aaaaaagtag aaattctata
    10201 tttaccttta ac
//
LOCUS       DBKOIJ010000102        10092 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000102 DBKOIJ010000000
VERSION     DBKOIJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.104"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            240..2075
                     /gene="tdc"
                     /locus_tag="ACV5NJ_001970"
     CDS             240..2075
                     /gene="tdc"
                     /locus_tag="ACV5NJ_001970"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HHY6486025.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2380..3858
                     /locus_tag="ACV5NJ_001971"
     CDS             2380..3858
                     /locus_tag="ACV5NJ_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHY6486026.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3824..5155
                     /locus_tag="ACV5NJ_001972"
     CDS             3824..5155
                     /locus_tag="ACV5NJ_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHY6486027.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5269..7821
                     /locus_tag="ACV5NJ_001973"
     CDS             5269..7821
                     /locus_tag="ACV5NJ_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HHY6486028.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7828..9267)
                     /locus_tag="ACV5NJ_001974"
     CDS             complement(7828..9267)
                     /locus_tag="ACV5NJ_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486029.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9242..9424
                     /locus_tag="ACV5NJ_001975"
     CDS             9242..9424
                     /locus_tag="ACV5NJ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486030.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9613..9975
                     /locus_tag="ACV5NJ_001976"
     CDS             9613..9975
                     /locus_tag="ACV5NJ_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HHY6486031.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3209 a         1657 c         2141 g         3085 t
ORIGIN      
        1 tgtttgttcg ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc
       61 gcttttttgc gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc
      121 tacgtggatt tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg
      181 ttacagataa acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa
      241 tgaaagatat ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt
      301 ataaaatgtt gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc
      361 cttcagatat gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc
      421 gcgaccatat gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc
      481 catggcattc agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt
      541 tattagctta caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta
      601 tggcaacttc gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga
      661 cagacggatg gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt
      721 acgcccgttg catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga
      781 aaaaaatgtc tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg
      841 aaagctttac ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa
      901 atatccaaag attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg
      961 cgttagatat ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt
     1021 atcgaatgga tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc
     1081 caattctagg tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag
     1141 ataaaatcgt ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg
     1201 ttgatgccgc atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg
     1261 tgccgtatgc ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa
     1321 cgattgacaa agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga
     1381 tagatcctca taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata
     1441 aaaatatgag aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg
     1501 ctccagatat gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg
     1561 cagtctggac agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg
     1621 gggcttcaat cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg
     1681 ttaaaggaaa aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt
     1741 gggtattcaa agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa
     1801 tgttcgatcg gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc
     1861 atactacatt tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg
     1921 gtctaacgaa agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca
     1981 tgactcctta tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag
     2041 caatggaaaa gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa
     2101 caagagacat atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct
     2161 gtttttcata tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc
     2221 gttcgtttca tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac
     2281 tgggttttgt actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga
     2341 aaacaaaaac atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca
     2401 aagcaaatca cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga
     2461 aatattccag atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta
     2521 ttattgtacg cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa
     2581 aaaggtggtc cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc
     2641 tcatggctgt tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct
     2701 ccgttgtttg gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta
     2761 gttgtcattt tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca
     2821 aaaatcggcg gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc
     2881 ttattagggt ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt
     2941 ttttcatggg acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg
     3001 ccgatcatgt ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa
     3061 aatccagtta aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc
     3121 aatattttga atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat
     3181 aatatcgcgc aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg
     3241 aatctgttta gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca
     3301 ggaccagcaa agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat
     3361 ttttggaaga cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt
     3421 atctcaatct ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg
     3481 ctcgtaaatt caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt
     3541 ttgagattgc gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag
     3601 aaaaagagca atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt
     3661 tctgtcggac tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc
     3721 agtgtcattt tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa
     3781 caggaagtcg ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat
     3841 gaaacggact ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac
     3901 aggattattt ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt
     3961 ttatttgata tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt
     4021 agcaaatagt acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg
     4081 ggcatttttt gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt
     4141 aacagcaata ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg
     4201 aggtagcgca ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga
     4261 attggaatct tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg
     4321 tataggattg agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg
     4381 acagatcgat gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt
     4441 tttattcagt tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc
     4501 gcaaaagaat ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat
     4561 aggaacgatt ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag
     4621 tcctttagtg actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct
     4681 aatgattttg gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat
     4741 tttatatcaa acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa
     4801 aggcgtacct ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct
     4861 acattttttt gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag
     4921 ttgttttcta tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa
     4981 gaaacggaac actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat
     5041 tgctttcagc ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg
     5101 gtttgtattc ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca
     5161 gatacatttt gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa
     5221 aacttccctg cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac
     5281 agattgacgg tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa
     5341 gaagaacgac agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag
     5401 acaaaacgat ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc
     5461 attgcctctt ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc
     5521 gtagtgatcg tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg
     5581 aatggtttga agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa
     5641 aaaatccctg cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc
     5701 gttccagcag atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg
     5761 acgggagaat cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc
     5821 ggcgaccaga aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt
     5881 gtcgttgtag aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg
     5941 gtcgaacaac aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt
     6001 caaggcattc tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg
     6061 gattggcata tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga
     6121 ttgccagctg ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag
     6181 gcgatcatca aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca
     6241 gataagacag gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa
     6301 gaagcctgcg tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag
     6361 aaaacagaaa atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca
     6421 gccaatctac ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat
     6481 atggcaacga tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa
     6541 gtattatttg ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct
     6601 gcagaatttg cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg
     6661 aaacaggaac tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg
     6721 atcgatccgc caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc
     6781 tcagtgaaaa tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt
     6841 ggcttaaaac atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa
     6901 gagttgattc agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa
     6961 ctgcgaattg ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc
     7021 gtaaatgatg cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt
     7081 agtgaagtaa gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt
     7141 gcaaaagccg taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc
     7201 ttcttgccaa ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc
     7261 ccgctacctt tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta
     7321 tcttatgcac tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat
     7381 ccaaagcaag gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg
     7441 atcatggttc ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc
     7501 attcttctgc aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg
     7561 tccaagccgt cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt
     7621 atccttgctt tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt
     7681 acagtaagtt taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc
     7741 ttagttgtag aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca
     7801 gctagagaat acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa
     7861 acgattgagc tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga
     7921 atctagaggg aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc
     7981 ggttagttcg ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg
     8041 caagaacgcc ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg
     8101 tttgatagaa aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg
     8161 ttcattgatt tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa
     8221 gtgtgcatat tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc
     8281 aatcaatatc ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga
     8341 tgtttttttt agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag
     8401 gacctctaaa aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt
     8461 gtaattccaa ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata
     8521 aataccttcc gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct
     8581 gttggtatgg atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa
     8641 gaaccaacat gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt
     8701 tcttaaaaat tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt
     8761 atgagtgaaa ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag
     8821 aatacaagct gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt
     8881 gattttagtc acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc
     8941 agggacagca actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg
     9001 tttgaaaaaa atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat
     9061 tcctgcttga tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag
     9121 gtcagaaaaa atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat
     9181 ctttgtatga ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt
     9241 aatgattttt ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga
     9301 gaaaataaac ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc
     9361 atttactgat gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa
     9421 ataggtgggc aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc
     9481 ggattgaaca tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta
     9541 cttgtattca tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag
     9601 agatggaagc agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat
     9661 ttgtctccag aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa
     9721 agcaaaaaat ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca
     9781 agtgaccata ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt
     9841 ttgcgaggaa aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct
     9901 attgatctac gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt
     9961 aaatcaacgc aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct
    10021 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt
    10081 attttctaag aa
//
LOCUS       DBKOIJ010000103        10081 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000103 DBKOIJ010000000
VERSION     DBKOIJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.105"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1154
                     /locus_tag="ACV5NJ_001977"
     CDS             <1..1154
                     /locus_tag="ACV5NJ_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6486032.1"
                     /translation="VYLIYYLLMVIMAVIAQDTLHASLSQAGFASGIYIIGTLLARLF
                     MGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYLVRFLNGFGYGTVSTATNAI
                     VTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMTDFRFIIWFSIVLVFFVTIA
                     CLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIAFLMGLSYSSVLSFLSSYTK
                     VIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVMYPSYLFLTAGLFLLSVTTN
                     SVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIALSTYFIGLDLGLGVGPYLMG
                     MLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEGELIESLKNEI"
     gene            complement(1302..1667)
                     /locus_tag="ACV5NJ_001978"
     CDS             complement(1302..1667)
                     /locus_tag="ACV5NJ_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HHY6486033.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(1814..2587)
                     /locus_tag="ACV5NJ_001979"
     CDS             complement(1814..2587)
                     /locus_tag="ACV5NJ_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHY6486034.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(2761..3621)
                     /locus_tag="ACV5NJ_001980"
     CDS             complement(2761..3621)
                     /locus_tag="ACV5NJ_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HHY6486035.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3621..5039)
                     /locus_tag="ACV5NJ_001981"
     CDS             complement(3621..5039)
                     /locus_tag="ACV5NJ_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HHY6486036.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5096..5545)
                     /locus_tag="ACV5NJ_001982"
     CDS             complement(5096..5545)
                     /locus_tag="ACV5NJ_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6486037.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(5990..6253)
                     /locus_tag="ACV5NJ_001983"
     CDS             complement(5990..6253)
                     /locus_tag="ACV5NJ_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HHY6486038.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6352..7647)
                     /gene="rho"
                     /locus_tag="ACV5NJ_001984"
     CDS             complement(6352..7647)
                     /gene="rho"
                     /locus_tag="ACV5NJ_001984"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HHY6486039.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7644..8915)
                     /locus_tag="ACV5NJ_001985"
     CDS             complement(7644..8915)
                     /locus_tag="ACV5NJ_001985"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HHY6486040.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9080..9949)
                     /locus_tag="ACV5NJ_001986"
     CDS             complement(9080..9949)
                     /locus_tag="ACV5NJ_001986"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HHY6486041.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         2765 a         2167 c         1782 g         3367 t
ORIGIN      
        1 ttgtctacct gatttattat ctgttgatgg tcatcatggc agtcattgcg caagacacct
       61 tacacgccag cttgagccaa gcaggttttg cttctgggat ctatatcatc ggaacattgc
      121 tggcacgctt attcatgggg aaacagttag aactatttgg tcggaagttt accttaagag
      181 gcggcgcgat cttctatctc ctttcaacat tagcatatct ttatacgcca actatcgggg
      241 cattgtattt agttcgattc ttgaatggat tcggctacgg tacagtttct acagctacaa
      301 atgctatcgt tactgcatat atccctgcat ctaaaaaagg ggaaggcatc aattattatg
      361 gattaagtac tagtttagct gccgctgttg ggccattttt aggaatgatt ttgctgaata
      421 tgactgattt tcgtttcatt atctggtttt caattgtact ggtcttcttt gttacgattg
      481 cctgccttgt ctttcctgta aaaaatattc cattatctga agaacaaaaa atatctcttc
      541 gctcatggaa tatcaatagt tttattgaaa agaaagcact tttcattaca ggaattgcct
      601 ttttgatggg tctttcttat tcaagtgttt tatcattttt atcttcttat acaaaagtaa
      661 tccacttagt tgctgctggg tcatttttct tcgtcgtata tgctttggtc atcacattta
      721 cacgtccatt gactggaagg attttcgatg tcaaaggaga acaatacgtg atgtatccta
      781 gctatttatt cttaacagca ggattatttt tactgagtgt aacgacgaat agtgtgacat
      841 tgcttatctc tggagcatta gtcggcctag gttatggtac gtttatgtca aatggacagg
      901 ctgtttgttt aaaaatcgtc cgcgaacatc gaatcagtat tgctttatct acctacttta
      961 ttggtttaga cctaggctta ggcgttggtc cttatttgat ggggatgcta aaatctctta
     1021 cttcatttag aggattgtat gtgattgccg gagtcattcc attgatatgt acagcactct
     1081 atcttgttaa tggccgaaaa catagtcatg aagcagaggg cgaattgatt gaatcattga
     1141 aaaatgaaat ttgatcttga tgaatagata aaaaaaaccg ttcagatctg aaaataatct
     1201 gagcggtttt ttgagtaaaa atagtttatg attattctta attaaacatc tatttatttt
     1261 taaagctttc atttatcgat aagaatagtt taactgttga tttattccat tttccgttca
     1321 tctggtacga tgccttgact tagcaaccac ttgtggaaga accattctgc cacaccgatc
     1381 aatagagcag gaatcaatac tttcatccaa atatcgactg tatcattcat aaaatataga
     1441 tagagccaca agataactgc agctgaacca gcatctgcga tcgtcgctgc gatgttccct
     1501 actttacgta agatcaacag atcccctagc acatagatca tgactacaat aattacccca
     1561 acaatcgttg aatccataaa gctaacaccg tataaacctg tcagcacgac ccacagaatc
     1621 gcccagatca tgattgcctt cactaccaac gccttcacat gtttcatttt ctgcactcct
     1681 ttctcctgga taaaaaagta agattatcta acaagataac cttttcatta ttataccatc
     1741 ggctgagaga agcgagcaaa gataaggtcc tatctaaaaa tgaggaagcc acttagaaca
     1801 ttcacgtaat aatttatccg tacagacatt cgatatggtg ttcctttact ttcgcatact
     1861 gttcttttgt cagttcttta cttgtcacca gataatcgat ttcatgaaaa gcaatggctt
     1921 gatgcatgaa tttatgtgca attttttgct gtgtaactaa aacggcaagc cggtctgatt
     1981 gttttctaaa agcttttttg accattgcct ctccttcatg agtttctgtt actccttggc
     2041 tgaaatctag cccgcgacaa ctcattatcg ctaaatccgc ataatatgac aatgcatcgt
     2101 tataggcttt tgaaccgttg attgttgctc gtttcggaac aattgcccct cccaatacag
     2161 atacttttgc cgttgtatat tcactcaaca tagcagctgt gacgatgcca ttcgtaatga
     2221 tctgcaaacc atcaaatctt gctaggtttc tgactaagtg taaagaggtt gtactggaat
     2281 ccaagaataa gaccatatca tctcgaatca aagaagaagc tgtatctgca atcttacgtt
     2341 tttctgtttc ttcttctaat ttggtgatct tcatcaggta aggcacgtca atttttcgat
     2401 tgtcaataag catggcgcca ccgcgaactc tacggatcaa tccacttttt tccagaaaaa
     2461 caaggtcacg tttaatcgtg gcttcgctat aatttactaa ctcgatcaaa tcggtgactc
     2521 ttgcaaattg gttgtttttt agataattca caatctcctg ctgtctattc tccacttcat
     2581 tcaacataaa atcaccccat ttactttata tagtaacagg aaaaaagagg ttgtgacaga
     2641 agtgattggc ttcgagaaat aagaagaaat ttttcgaaaa aactacttct gaacatcgtg
     2701 tgtggttaag agggtatgac aagactattg tcataccctc ttgaccacac atttttattt
     2761 ttagtataaa ctagccttgc ctactgtgtt caaaatttcc attttatgtt tgaccacttc
     2821 ttgtaccttt ttattagctg ctggataaat tgcattcggc tcgtacatcg atggattttc
     2881 actcaatatt tctctcaaag cattgaaaaa gacgctttta agatctgagc tcaaattcac
     2941 tttggcaact ccgtatttta ctgcttcgct gacttctgcg tccggattac ctgaaccgcc
     3001 atgtaaaaca aatggaagat cgatttgttg gttcaattct ttcagtaagt cgatatttaa
     3061 ttttggagtt ctgtttttcg gatacaagcc atgcgctgtt ccaattgcta cagcaagtgt
     3121 atcgattcct gtttctttca caaaaatctt cgcttgatca ggatctgtat aaatgattgt
     3181 ttcagcacct ccttcagcag aaccattatt cccaatcgtt ccaagctcag cctccactga
     3241 gacatttacc gcatgagcta acgcaaccac ttttttcgta atctgtacat tgtcgtcgaa
     3301 tgatttcaaa gacgcatcga tcatcacgga agaatagccg tttcgaatcg cccgtaagac
     3361 atcttctatc gtaccgccat gatccaagtg gataacgatc ggcagtttac ttgtataagc
     3421 ataggctctt actgccgcaa caaactcatc acttaaatag gcgatttcat caggatgaat
     3481 ttctaagatt actggtgctt ctaatctctc tgcttcttcg atgattgctt tcatcatatc
     3541 atagctgcaa atattgaagg ctggtactgc aaattgattc tctttagcca ctttcaataa
     3601 ctctttcatt gtatataaca ttacgcttcc acctcatttg ttagattttc atttttcgtc
     3661 cacttgtctt gttttttctc tttcactgtt tttttcaata gtccaagcat aaaggctgta
     3721 gtgactgtac ctgatgcaat tgccaagaca tacataggta aattgttcat aacatttgga
     3781 attagcatca cccaaatccc tccatgaggt gccattgaag taactcctac tgccatggaa
     3841 atccctgcag cgactgctga acctgtcatc aaactaggta tcacgcggat cgggtcagat
     3901 accgcaaacg gtatagcacc ttctgtaata tacgaagcgc ctaaaatcca gcatgattga
     3961 cctgaagtcc gttcttcttt ggtaaattta tgtttgaata ataaagtggc taaagcaata
     4021 cctaggggag gtgtcattcc tgcagccata caagcagcaa tcggtgcatt tactccagct
     4081 gacatcaaac cgatcgagaa agtcgaagtc gatttattga tcggcccacc catatcagct
     4141 gccatcatga tcccgataag taatccgaac aatagcccgg atgtatttcc caagctattc
     4201 agccaatttg tcaaagcatc caacacccat gtgatcggtt gttcaatcac aaataccatc
     4261 aaaagcccaa taattaagat cgataaaaag gggacgacaa tcagctgata aatagcagaa
     4321 aattcttttt ttacgctaat tttatttgcc agaaaatcac agatataccc tgctagcacg
     4381 ccaccaatca ttccgccaag aaaacctgaa ccacctgtag aggccagtaa tccggcaaca
     4441 attcctgggg caaaacctgc ttttccagca atcgatgtag caatccccgc gcctaacgca
     4501 ggaaccatca tggcaaacgc tgtatctcct ccgatccttg aaaatgcagc tgctaacaca
     4561 ttatagtctg ctgagttggg atcagccgct gtaatcccaa aggcaaaact cagtgcgatg
     4621 atgattccgc ctgcgatcac aaatggaagc atgtaattca caccattcat gaaatgatta
     4681 tagattgtcg gctttttctc atttgcagct ggttcttttt cattagacac aacgaatacg
     4741 ccaatatctc tcaatgcttc gtctattact acttgcggtt ctttgattgc tcggcttgta
     4801 gagacacgct ttactttttt cccggcaaat cggtccaatt cgatttcttt atctactgca
     4861 agaatgattg catctgcctc tttgatttct tgttcagata actgattttc tgtttttaca
     4921 ccttgtgttt cgaattttgc ttgatggttt agatttcttg ctgttacttc taatttttct
     4981 gccgacatat aagtatgagc aattccagtc gggcaagcag ttacaccaac gattttcatg
     5041 attattttcc tcctttttct tcactatcga tactcggaca aagactctac tattttcatt
     5101 cttgactaat ctgtctgatg atatctgctg gcgtagtggc ttcttttatt ccctttctaa
     5161 aatctgcatt catcaatcgt ctggagagtg tgctgagaat ctctagatgc atatctgctg
     5221 atttttcagg aactacgatg gcaaaaatat attcaattgg catttcatct atcccgctat
     5281 agtccgttaa tttggctgta tgaaaaaaac acagtgctgg ttttgtcaca tatttgcttt
     5341 ttgcatgggg aatcccaaat ccatcttcta ttcctgtgga gatctcctcc tctcgtttga
     5401 atagtgcctt aacgactttt tgaacatttc ttaccttatt tgtttttttc aacacatccc
     5461 ctattcgctc aaataatgat ccttttgaat ctatcgatgg atcgaaaaca atcagttctt
     5521 ctgcgatcaa ttgatcaaac ctcataaaaa acctctcccc cacaaaaaat gttaacgttt
     5581 tcttggaact gaggtcagta tagcatcgct agctgtaccg ttcaatatta tgaatgaaca
     5641 gaggaaaaag acccatcatc cgcgtgtata agtcattaaa atgaacgatc tagctcacaa
     5701 acaatcgcaa gtctataaca aaacaaaaag ccttccttga aaggaaagct tagcttctaa
     5761 ttttaggtaa atggtttttt acttcgaaat aacccctctt catttcgtta atataaggtg
     5821 tttttatcaa gtggataatt aaaactatat actagttttt cattgttttg tgcattatga
     5881 tcctacgact ttttcagcat caaacgaagt tttaaattgg caaaaaacat aaaaaaacag
     5941 actatcccta tcagatagtc tgctaaacgt tgagttatca gcgtttttat tattccgcag
     6001 cgtttgcgtc tttgagaccg tattttttgt tgaaacggtc cacacgtccg tctgcttgtg
     6061 tgaatttttg acgtccagtg tagaatggat gtgagtcaga agttacttcg acacggatga
     6121 ctgggtatgt attaccgtct tcccattcaa ctgtttcttg tgaagttttt gttgaacctg
     6181 acaagaattt gaaaccagtt gttgaatcca tgaatactac tgggtggtaa tctggatgga
     6241 tgttttcttt catgattttt tcgctccttt gccctgctcc atttgctgaa ccagagttga
     6301 tttgtcgatt tacaacatta acatcttaac atgtttcagt caaggttgca attatctttt
     6361 cagatttctg gtcggatttt ttttaccaaa ggagacatct tgaaattctt tgaaaaattg
     6421 ctgattgttt tttgttttac gcaaaaagcg gatcagttgt tccgtatatt ctaatgaatc
     6481 tccagtcata ttttttcgaa gtttccaaat ttcttctaat tcttcacttg gcatcaataa
     6541 ttcttctttt cgggtacctg attttttgat atcgatggct gggaagatcc gacgttcaga
     6601 taattcacgt gaaagttgta attctagatt tccagttcct ttgaattctt catagatcac
     6661 gtcatccata cggcttcctg tatcgactaa agcggtcgct aaaatcgtca aactgccacc
     6721 ttcttcgatg tttcttgctg ccccgaaaaa tttcttaggt ttatacaatg cggctgggtc
     6781 aatcccgcca ctcaatgtac gtccgcttgg tggaacgact aagttatagg cccgagcaag
     6841 tctagtaata ctatccatca agataacgac atctcgttta tcttcgacta aacgcattgc
     6901 gcgttccaat actaattcag aaactcgagt atggttttgc ggctgcagat caaatgtaga
     6961 agataccacg tcgcctttca cgcttcgttc tagatccgtt acttcttccg gtcgttcatc
     7021 aatcagtaga acgattaatt ctacttccgg atagttttcg gtgataccat ttgcgatttc
     7081 ttttaataca gaagttttcc ctgcttttgg cggtgcgacg atcaatccac gctgaccaaa
     7141 accgattgga gaaaagatat cgatcattcg ggtagatagt ctttgcggtg ttgtttccag
     7201 ttgaagctgt ctttgcggat aaagaggtgt taaggctgga aaatgcggac gttcttttgc
     7261 ttcttccgga tcttttccat taacgctttc cacgtgcatc aaaccataat aacgttccga
     7321 ttctttaggt ggacgggctt ttcctgccac tttgtcccca tttctcaaac tgaatcgtct
     7381 gatttgtgag gcagaaatat agatatcttc tgcacttggt ccataattga ttggccgtaa
     7441 aaaaccgtaa ccatcttgac caacgatatc caatattcct tccatataat aaaatccttg
     7501 tttctctgct tgtgcacgaa tcacagctag agataattcc tttttattca tcttactgta
     7561 ataaggaatt ttaaattctt tggcataact ataaacttct tttaacgttt tgttctctag
     7621 ttctgccatt gtcaaataat cgctcattca gctcccactt cctctatcat ttcaattgat
     7681 gcccctaagg ctgttagttt ctcgataata tgatcataac cgcgtaagat ataatctaca
     7741 ttgtaaatcg tcgttgtccc ttctgccaat agtccagcaa tcactaaaca agcacctgcc
     7801 cgaagatcag aagccacgac ttctgtgccg tgaagtttat ttggaccatt cacgatgatc
     7861 atatttcctt ccacggtaat atcagcgccc attcgtgcta gttccggcac atggttcaca
     7921 cgtttcgcat agatcgtatc gatgatttct gaagtgcctg tagtcatcaa tagtaacgca
     7981 gtaagtggct gctgcagatc tgtggcaaat cctggataag gataggtcat gatgtttgcg
     8041 ggttttaatt ctttcgaggg gtaaacttcg atttcatctt cgctgatttt catatggaca
     8101 ccaatttcct gcaattttgc tataaagctt tccaaatgtt caaagatcac atttttcact
     8161 ttgataccat ttcctactgc agctgctaat gctagatagg ttcctgcttc gatgcggtca
     8221 ggaatcatga aatgccgaca gccatgtaat gtttcgactc cttcgatacg gatcacatct
     8281 gtacccgctc ctcgaacttt tgcccccata ttattcagta aagttgccac atctatgatt
     8341 tccggttctc gtgcagcgtt ttcaattact gtctgtcctt ttgcttttac agcagctaac
     8401 atcacattga tcgttgctcc gactgaaacc acgtccatga aaatccgatt gccacgcaat
     8461 cctttatttt ctgtccgtaa atacatggca ccatgttcat ttgtcacttc agcacctaga
     8521 gcttcaaatc ctttgacgtg taggtcaatc ggtctagggc ctaaataaca gccacctggc
     8581 aggcctacta ccgcttcgcc aaatttacca agcagtgttc ccataaaata ataagaagca
     8641 cgcaaactat tgatttttcc gtgcggcatc ggaatcgata cgacttctcg aggatcgatt
     8701 tctagtatgt tgttggaaaa atggacagtt gcgcccatga tctctaaaat ttcaattaat
     8761 gaatgaacat cttgtatgtc cggcacacct tccaagatca ctggggaatc agctaagatt
     8821 gcagctggaa tcaatgcgac tgcactattt tttgcaccgc tgatcgttac ttcaccagct
     8881 aaaggtcggt ttcctttgat tacgattttt ttcatctgtt tccaccttgt ttattaatct
     8941 tttttcatgg atatgcttgt attttatcat agataaaaaa aagaaacgag catggtctgc
     9001 cgttttaagt aaaaaagaat ggagagcgac ttcaaaagtc gttctccatt cctatatatc
     9061 gtatctgtta tgtggatgct tacgctttgt tagctgaacc gaaccagtcg atatgagatt
     9121 tagcatcttc aacgattgct gctttacctg gagctaataa tttacgaggg tcgaatcctt
     9181 taccttcttt atctttacca gcttcgatat atttacgagt agctgcagca aatgataatt
     9241 ggaattcagt gttaacattt acttttgaga tacccattga gatagctttt tcgatttgat
     9301 cttgaggaat acctgatccg ccatgtaata ctaaaggcat gtctggacct acagcatcag
     9361 caattgcttg taagtggtca aaagctaatc ctttccagtt ttctggatat tgaccatgga
     9421 tgttaccgat accgcaagct aagtagtcga tacctgtagc aaccatttgt ttgcattctt
     9481 caatatcagc taattcgccg ttaccgatga ttccgtcttc ttctccgccg attgaaccaa
     9541 cttcacattc aactgagata ccttttgcat gtgctttttc aacaacgtct ttagctaatt
     9601 tcaagttttc ttcaaatggt agatgtgaac catcgaacat tactgaagta tagcctaatt
     9661 cgatacattc caaagcagct tcgtaatcac catggtctaa gtggatagca actggaacag
     9721 tgatgttcat tgcttcaact aaatcagtga ttaaatcttt acagattttg taaccaccca
     9781 tgtatttagc tgcacccatt gatgtttgga taagtactgg tgcttgtttt gcttcagccg
     9841 cttcaaggat tgcttgagtc cattccaagt tgtttgtgtt gaatccgccg acagcgtaac
     9901 cgcctttacg agcagctttt aaaaattcag ctcctgatac tactggcata ataaaattcc
     9961 tcctaagttt tgtaaaacta aatttgttaa gacaattctt aaccaacgac cctattttac
    10021 caaacatttc ggtagttttc tactaaaatc aatgaaaatg aaaattttca agaagttttt
    10081 t
//
LOCUS       DBKOIJ010000104         9811 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000104 DBKOIJ010000000
VERSION     DBKOIJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.106"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(34..2154)
                     /locus_tag="ACV5NJ_001987"
     CDS             complement(34..2154)
                     /locus_tag="ACV5NJ_001987"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HHY6486042.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACV5NJ_001988"
     CDS             2384..3562
                     /locus_tag="ACV5NJ_001988"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HHY6486043.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACV5NJ_001989"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACV5NJ_001989"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HHY6486044.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACV5NJ_001990"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACV5NJ_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HHY6486045.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACV5NJ_001991"
     CDS             complement(4915..6168)
                     /locus_tag="ACV5NJ_001991"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHY6486046.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACV5NJ_001992"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACV5NJ_001992"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HHY6486047.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACV5NJ_001993"
     CDS             7746..9092
                     /locus_tag="ACV5NJ_001993"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HHY6486048.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACV5NJ_001994"
     CDS             complement(9400..9786)
                     /locus_tag="ACV5NJ_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HHY6486049.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DBKOIJ010000105         9701 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000105 DBKOIJ010000000
VERSION     DBKOIJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.107"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            87..854
                     /locus_tag="ACV5NJ_001995"
     CDS             87..854
                     /locus_tag="ACV5NJ_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HHY6486050.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1046..1444
                     /locus_tag="ACV5NJ_001996"
     CDS             1046..1444
                     /locus_tag="ACV5NJ_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HHY6486051.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(1751..2125)
                     /locus_tag="ACV5NJ_001997"
     CDS             complement(1751..2125)
                     /locus_tag="ACV5NJ_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486052.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2147..2497)
                     /locus_tag="ACV5NJ_001998"
     CDS             complement(2147..2497)
                     /locus_tag="ACV5NJ_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486053.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(2514..2909)
                     /locus_tag="ACV5NJ_001999"
     CDS             complement(2514..2909)
                     /locus_tag="ACV5NJ_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HHY6486054.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(2976..3161)
                     /locus_tag="ACV5NJ_002000"
     CDS             complement(2976..3161)
                     /locus_tag="ACV5NJ_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HHY6486055.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3243..3497)
                     /locus_tag="ACV5NJ_002001"
     CDS             complement(3243..3497)
                     /locus_tag="ACV5NJ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486056.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(3519..3764)
                     /locus_tag="ACV5NJ_002002"
     CDS             complement(3519..3764)
                     /locus_tag="ACV5NJ_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486057.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(3771..4055)
                     /locus_tag="ACV5NJ_002003"
     CDS             complement(3771..4055)
                     /locus_tag="ACV5NJ_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486058.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4158..4340)
                     /locus_tag="ACV5NJ_002004"
     CDS             complement(4158..4340)
                     /locus_tag="ACV5NJ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HHY6486059.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(4546..5031)
                     /locus_tag="ACV5NJ_002005"
     CDS             complement(4546..5031)
                     /locus_tag="ACV5NJ_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486060.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVDTKVTKIYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            complement(5198..5464)
                     /locus_tag="ACV5NJ_002006"
     CDS             complement(5198..5464)
                     /locus_tag="ACV5NJ_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HHY6486061.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5466..5687)
                     /gene="relB"
                     /locus_tag="ACV5NJ_002007"
     CDS             complement(5466..5687)
                     /gene="relB"
                     /locus_tag="ACV5NJ_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HHY6486062.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            5913..6731
                     /locus_tag="ACV5NJ_002008"
     CDS             5913..6731
                     /locus_tag="ACV5NJ_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HHY6486063.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            6744..7031
                     /locus_tag="ACV5NJ_002009"
     CDS             6744..7031
                     /locus_tag="ACV5NJ_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486064.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(7213..7566)
                     /locus_tag="ACV5NJ_002010"
     CDS             complement(7213..7566)
                     /locus_tag="ACV5NJ_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486065.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(7586..8641)
                     /locus_tag="ACV5NJ_002011"
     CDS             complement(7586..8641)
                     /locus_tag="ACV5NJ_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HHY6486066.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            8857..9255
                     /locus_tag="ACV5NJ_002012"
     CDS             8857..9255
                     /locus_tag="ACV5NJ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486067.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            9258..9431
                     /locus_tag="ACV5NJ_002013"
     CDS             9258..9431
                     /locus_tag="ACV5NJ_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486068.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3168 a         1510 c         1687 g         3336 t
ORIGIN      
        1 tctcgaaaaa tcaattacga gttatttgcc ttatgtgaac ctttatgatt aacaactgaa
       61 aaaatgcgtg aaggaaggaa aaacttttga aaaaagatga actgacaaag tacaatgagg
      121 agttcttagc aacaattgac atgaaaatgg atttaaagtc gtatgaactt tatcaattgc
      181 tgttcagtta tatctcaccc acaaagctaa agaaaacagg cgtagtagat ttacctgtta
      241 aagcgattat taattacatg gatgttgatc aaaatcttga aacggaacta aaaaccatag
      301 ttaacacttt gcaaagacaa gcagtttatg aaatttcgaa tctagacaaa aaatataaga
      361 cgtatactac tgtatctgct attgatcgga ttcattggga tttagaagag catagtgtat
      421 ctttcagttt ttcagaagat gtaattccct taattgtctc aatcaaaaag aagtatccgt
      481 tctttgtttt tgaagactta gcatcttttc aaaaaaaata tagcatcgtg ttatacaaat
      541 atttggcgat gtattataaa aaatatctta tcgccaaaaa taaaggagat tttgagaaag
      601 ctgaaatata taagaatccc ctgattgaaa tagttaaaat tcgtgagatt acgaaaacag
      661 aaaatgagta taaaacaata tcgaattttg atagacgtgt tttaagggaa tcagttgagg
      721 aaattaatga aaaatcaatg ctgaatattg attttgctaa agttagaaaa ggcagaaatt
      781 acaattttgc tgtccagttt aatatttcta gtgagtttga tgaaaaaact tatagagaaa
      841 aaaaattagg ttaatgcaaa atatatctta ctcgtttgaa gatgcttcag acgtatgcta
      901 agatatatag tgccttggga gcaaggtatt aatctccttt agaggttaat tagcttgtga
      961 tcgcctatca atttttttgg taggcgattc ttttttttga aacaaaaaag caaaaattat
     1021 tttttaatta gagaggtgca atgcgatgaa atactttttt tgtgcagtat tcgaatggaa
     1081 aggaaatgaa tatgcagtta aattcccaga ttttccagac ttatatacac atggtgaaac
     1141 tttagaagat gcttttgaga tggcgcaaga agtattggaa ggatacttgg tgcttttaga
     1201 gttgcaagaa aaagaaattc catcgcctac gcctgttcaa gagttaaatg caagaatcaa
     1261 gcgatctgag aatgttaatt tatttgtcgt aaaagctaat acgaaagttg ctaaagctcg
     1321 aaacaagcaa aattatgatc gaaaatcagt gactgtggaa cactatttaa atgttttagc
     1381 ggctgaagag aatattaatc tgtcgaaagc tttgaatgat ttgttgagag aaaagttgat
     1441 ttgatgtctc tcagatgatc atattaagca aaaacagctt gttttaggac gtatggattt
     1501 aagaaagaca aattatcaaa gaatatatta aaagctctca gaagccttct gagagctttt
     1561 aaattgctat gtattgtact gcatctgctc aaattgattt tcttttcatg cttaccatgt
     1621 atctaatttt ccatcaagta atttttagat atgtattgag taataaatag caaatttgct
     1681 tgatataagc tcgaattagg tgctttgtaa tgtgttagga taaaattata caagaaaatg
     1741 tcaaaagctc tcagaacgct tctgagagct ttttatcggt aggcttgttt aacgtgatct
     1801 tgaaggatac ccttttgatg tttctctcga tccctgcatc gctaattact aagtgttctt
     1861 caagtgtgat gtaggtatat aaattaagca tcaaaatggt gtagataagc aaaaaactcc
     1921 ctaaaactaa gtgttgatta taaaaatctg acttataaaa aagaactgag aatgttagtg
     1981 ttgattccca aaaaagaaac aaaattttgt atttccaaag atagtggatc aagcgctgtg
     2041 ctttacccat ctcaaataat ttataataac ctgctaattg atcagcaagc gtatcgttaa
     2101 cggtttctgt ttgtgtttgt ttcatctttc tcttctcctg tttcaattaa tctaaaattt
     2161 cagattgagc aagcaactca ttgtggtagt cggtttgaaa ttcaaataac aattttgcct
     2221 tcttaacgag gtttaaagcg ttcgttagtg attcacttaa tgcttcaaca tctttaactg
     2281 attttgcatg tttcattttt tcagtgtgcg tatagcagaa ttcaggtatt gagttagggg
     2341 ttgcctgttc aataaacatc tcaattcgca agttattatc ctcaaaaata attagatcat
     2401 tggcaaagtg tttggtacta acgctaatgc cagttgtatc ttttaataga ttattgatta
     2461 acgttcgaga gaaatagttt aagtgtgtag ctttcatagg ttcggtctcc tttttacttt
     2521 tcaagcaact cttcttccag tgctttttga agtagttgag agaagtttat gtttttctcc
     2581 ttccctaaaa gaactaacca ttccggaaca ctaacatttt ttctaattgt ttttgtattt
     2641 aagcttcttc ggaaaagttt aatgtttagt tggatgatta ctaccacttg gttggctttc
     2701 aaagttaatt cttccactgc tgatggttta ggaaattctg catctggtac actatatagc
     2761 gctaatccta aagcatcctt agcgtttaaa tatgcttctt caagactatt tccaaaggta
     2821 gatatttccg gtaagtcagg gaaaacgatg ttataagctt ctccttcttt ctcgaagatt
     2881 gccggataaa aaatattttt tgtcttcatt tcctcgctcc ttttctagtg taagagagga
     2941 tagtatgcct aataccaaat aggaaggcaa ggctgttaaa gccctgcttg cttccagata
     3001 ttagcttctg ttcctagtgg tacatcacca ttgtggatcg gaacaacgac cgttctttta
     3061 gtcttggaat tataaaattt tgcatgactg ccagtttgtc cagtcttttt gaagccgtta
     3121 gatttgagaa tttttacaat ttctcttgct gtcttaggca tagctaccct ccctttacaa
     3181 aatctattat acacattgtt gtgtatggaa tcaatagaaa aagaggatgt tttattgcaa
     3241 aactaataaa atgatttatt ggttttttca gcaaattgaa tgttattagc gttgcaaata
     3301 ttcatgattt cccaagtatc atcagaagta taggtatcta agaattcatt gacagttacg
     3361 gttgattcgt atggttcata agtcaaagcg ttctccaagt cttttgatga aataatcaag
     3421 actttttcaa tttcctcccc ttcttcatct atgaaaacat attctaaagt gatttgttct
     3481 tcatcggtat aattcatatt gtgttctcct ttcttgtact aagaaaaatg gatatttcca
     3541 attgcatccc actcggcacc attgtggtat aggacgtgac cgagcgttac gcctttgaaa
     3601 tgttcttcta aatacatcca aacgcattca aggctagtac ctgctttgag attgtagaag
     3661 tctgcatcca ataccttttc agaagttatc ggatcaatgc gagttggtac ttcaataaat
     3721 tcagtccaaa tttctgcaaa agtcttacct tccaaagtcc acactcccta tcaattaatt
     3781 ccaaaagcaa atagagaaat caagcgaccg tcaatagtca cttcaacaac tgaaaatgcc
     3841 gttgaaacag ttttaacatc tacactttcc aagttcccga tgaaggaatc tttgtgcttt
     3901 agaatagctt tctttagctg tctgttatac acttgccgac tgttaaactt tttgaattct
     3961 cgatccttga tcagctgttt aacgatccct gcattaattg tataactttc tgcaatagca
     4021 acgtgagtag gttgctgtaa agtagctgtt agcatcttgt cactccttcc taaaagttaa
     4081 ttagcttgtt tacaaataca ttatacacat cggtgtgtat aatgtaaaga gaaaaaggtg
     4141 ctaatggatc agctgttcta aacaaaagaa aacgataaag taacaaataa agtcagcaaa
     4201 gttaaaaagg atgaaacggc tagtccggtt gctttgcttg tgtgattttc ggctttctta
     4261 tagatcaaat aggaaattat cagtgatatt ccagtaaaga ataatgtact agctgtcact
     4321 aaaagaaata ggttaatcat acgatacact ccttctgtaa tattggtatc agatacgttt
     4381 aaatgcgttt ctgagctgtt ttggatcaga tggtgatgtt tttagcccta ccctaaaaac
     4441 aaagcccaga aaagaattta aaagcgttcg tacctaatac gtttatattc atatacatat
     4501 acataaacat aaacgtgtat acgtttatgt ttatgtattt aagttttagg ctacttcctt
     4561 aacatctcct gctgatagtc gagtgaaccg agtgagtgat ccatcttgta gtcgaaaaat
     4621 aaagctacgt ttgagtttat actgatagac agcgacatac gatccatccc agattgagta
     4681 agttttacct gcaactaata agtcatcaag ttttggttta ttgctttctt ctttaattct
     4741 atctagttgt cgcttaacca ttttttcttg atcaccgaca aagaatttca accatttttt
     4801 cggaattttc ttaaagtaag caggactgtc atttaagaag tagattggtt tgtacatttc
     4861 atctcgaaag atgaagctgt caatatcgta ggttccttct gcatctttga aaaggataat
     4921 tccatagatc ttcgttactt tggtatctac gatttccaaa ccatttttgg cgtacccttt
     4981 cttttcttcg atcacttcat ttgctgatga ataatcacca taccatccca taaaaaatcg
     5041 ctccttttga aagttaatta gcttgtttac acttatatta tacacaccgg tgtgtataat
     5101 atcaagagga tattgttctt ttttaggtaa agtgggatcg taaacagtga ttttgttggg
     5161 ttaacaaaaa aacagacaag ccttatcagc ttgtctgtta cttataaact tcacttctgt
     5221 gaccaataga caatataaga attgtcactt catgctcgct aatatcagct attatacgat
     5281 atgcacctac cctatatcgc cagtagcctg atttattagc tgttagacct ttaccatgct
     5341 ttcttggatc atcagtgcct acaagatttt tctcgatcca gtttaggata acctttgcct
     5401 gaaacttatc catttttttt agcgcttttt gagcttcacg ttcatatctt acttggtaag
     5461 tcatcttata agcctaactc ttttagcata tcttcgtggg aaatactttg atcgttttcc
     5521 tcatgctctt tcattagttg cttgtaggtg gcaaagtcta ctaacgtttc tgctgtttgc
     5581 agggaattac tacgcataaa ttcagaaatg ttatctccgt tgaattcagc aactttttca
     5641 agaaaatctt tttcttcttt agatacacga atacttatta ctggcatagt ttatcaactc
     5701 cttttcaatt acattgtact acgattgtat gacaaatgca atagttatat atttgaatat
     5761 atacataaac ataaacatat acatatatat gtttatgttg tagaatataa aagcaaatat
     5821 aaatataaac acgtttatgt atataaacat atacataaac gtgtttatag tgtataatgg
     5881 agttacaaaa ggttaataag ggaggtttaa tcatggaaaa gaagttgtca atcattactg
     5941 gaaacattga aaaaggtgga acaggtaaaa caacaacaat ctacaatcta ggtggatatg
     6001 ctgtcgaaaa tttaggtatg aaggtgctgt atattgatga agataagtcg caaaacctat
     6061 caaaacgatt tcctgatgct ttcagtaaag cgaaaagaga gaatacaatc aatcatttat
     6121 atcttcatgg atcagctgaa cctttagcta taaatgagaa catggatatt ttagtagcag
     6181 gtgaagattt aaaacaagtt gagatgcaaa tgcgtgatat gcctaacaac agaaccctac
     6241 tgtttagatg gatcgttgtt aactatgaac agcttaaaga gaagtatgat ctaattctga
     6301 ttgatactca taatgatgca ggtctactag ctcaaaatgc ttgggctgta tctgatcttg
     6361 tcgtaggcat ttctgatcca tctaaagatg gttttgaagc tttgcttaag cttggtaaag
     6421 atattgatgc actagcaaaa cagttgattg atattaagac tggtgaaaca ctgatggaaa
     6481 ctaaatacgc tacgatcggt agcaggatca aacataatac taattcaagt cgtgagttcc
     6541 tagaattggt accaacgtta tctaattatc ttggatatgt acaagagaag gaattgctta
     6601 atgcaactag cttagaaggt aagaatattg ctgagttcgc taaggataag aatatctatc
     6661 gtaagaataa ggatttcttt gaaagcacgt ttacgctata caacaaaata attgatacgg
     6721 cacaagaata aggagtgatt gaagtgaaag aaaatagatt taaaaaattc gctgaagctg
     6781 aagtagaaga agttcctgaa gtggttgaag cagttgaaga acctaaacaa acacctaaag
     6841 aaacaacaag ttctttggta gctaaactaa aaagtgatag ttctgaaaag aagaaaaatt
     6901 atcaattcac gcttcaacca tcacaaagaa agaaactatc taaaatcagc gaaaagttag
     6961 gaattcgatc agattcagct ttacttggtg aaatgataga taacttgtat gatgaagttt
     7021 ttggtgagtg atcagtttaa agctgatcac tttttttatt ggaataagcg gttaggtata
     7081 gcttaactaa atttcgtata cgtgtttatg gatatatacg tatacatgtt tatatacatg
     7141 tatacgtata tgtttatgtt tgtatttgta cataataaaa aggctaggta ttgtaacctc
     7201 gcctttttat ttttaaagta agtctttgtt acttggtgca ttgaaccctt ttagcattgg
     7261 cttttctttc tttgctgaat gatcggatgt aaccgttgaa ggatgcgctt gttgaatagg
     7321 gttatgatca gttggtttat tctttacttc agctactttg ctatcgtttc tatcgttgcc
     7381 tgcttcaata attgtaactt cattgttctt tacacgccga tatagattca aagctttttt
     7441 gatcgtctga gagtatttaa cgcctagtgt ttcatattca gattcaagaa actctgctaa
     7501 aggtaactcg ctaacctttt ctctattgat tataactaca taacgctctg attttccctt
     7561 attcatgttg atctccttcg ctgttttatt gagttaaacc aaacttgtaa aagcctttta
     7621 cattagaaaa ttcactgtct tcaacaaaca aggtatgcgg aattttatct tttacaagtt
     7681 gtttgtcgat aatagaagcc ccaccaccag taaagacaac tttatctaag gttgtgaagt
     7741 ttttgaatgt agctaaaact tctgcaacaa ccatattggt gtatgttttt atttctcttt
     7801 caactacttc tgaaatatca aaactttcat ttccgcttgg tttaaataga tatacgcctt
     7861 tttctttgcc ttctcggata atcttttcaa gttggaaagc attaggactg atcggaaggt
     7921 caagcatatt tacgatacga ttatataaag taaaaatacc ggtatccttt tgttcacggt
     7981 tgttttcgtc aatttcaaaa ttttggacaa catcaaaaag tttcgtgcca ccaccaatat
     8041 caacaaaacc gatcttctga ctgatcaatg attcatcttg tacactacca tcatcagcaa
     8101 ggaggaggtt atatagtgtt ccaatcggct gtggcagtac ccaaacggta tctacgttta
     8161 tattataagc tttaccgtca acttcaactg aatgttgtcg tgtggcttgt tgcatcacgt
     8221 atttgacgat tgtttcggta tagtcggatg aaggcagacc taaaactaga ttaacggtta
     8281 aaacgtcatc agattgatct tctaaacgtg ctaggttagc taaagagaaa gataggatat
     8341 cttggtaggt ttcctgcttg tatctgctga tcccaaaacc tagtgattct atcacgtagt
     8401 catcttttcc tagctcgtgt atatccttcc caaaataata gctttcagta gtgtttacta
     8461 ggctatattc agaaacgttt agtgatcgtt ttaatgagga acctatatta gaaaaacgtc
     8521 ctgaattaat gatatttttg ttcaaaatac gagagggaag cgagagtgga gaaacgtgtg
     8581 tactcatttc ttttactgat ttattcccta agtcaacgga aaacaataat ggttctttca
     8641 tggtatgacc tctttctgtt tgtgtttatg gtatttcggt acgaaaatag tttacaccta
     8701 tttactaata gttgcaactg tttataagaa aaaaatgcaa tcaaaaaagt ataatcaatt
     8761 acaataaagt gtaaataaaa aggtgtaaat aaacacttgc aaatttatct tattatgata
     8821 ttataaaagc ataaattata ttaaggagtg atcctgatgc gattgagaag aaataagaat
     8881 tacactacta ttattttaga agattttact gatcaagaat tacttattgt cggtaaaaca
     8941 aaagcagatt ttatcgaaaa gggaaatgct tcaatagtaa aagcttcaaa ggttgctgtc
     9001 gaaaaatatt ttgattcttt agaagaaagt gctaaggaaa tttttaatgg agagaaagct
     9061 ggtagctatg agttagatgg tattaaattt agcttgatcc caacaactga aaatattatt
     9121 ccgaagaaat tatattatat cttttcatct gaaaaacata cttcaatgaa gccttgtatt
     9181 tttgattggc aatggttgat tgatgctgta ttggatgtaa aatgttcaaa atacaatatt
     9241 actttggggt gttaattatg agtatgctag ttagaaaatt accattatct gtaagagaat
     9301 ttctatttta cttggtctgc ttaatcgcat cagggtttaa gctgatcgaa catattttta
     9361 tctttgctta tgtattagca atgatcgcat ttttatctgg cggttggtta attatttatt
     9421 tgttaagcta agttttagat caaaaataat tagtaaatta aacgataaaa taaccgttaa
     9481 taaatgtaat gaaaggacaa cgcctaacaa tcagcttgta ataagattat aaaataacat
     9541 aatatcaaaa atagtgcttt ttaaaggaca aattaagtgt atcaacataa acgtgtatat
     9601 gtatatgttt atgttgatac tttttttgtc ttaatttttt atttttaaaa ttctatttac
     9661 taatgctgta tagatagtga tcagaaaaaa caaaatagcg a
//
LOCUS       DBKOIJ010000106         9648 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000106 DBKOIJ010000000
VERSION     DBKOIJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.108"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..470
                     /locus_tag="ACV5NJ_002014"
     CDS             <1..470
                     /locus_tag="ACV5NJ_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HHY6486069.1"
                     /translation="TNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDRGKVD
                     ISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENPVVDI
                     IGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            503..2731
                     /locus_tag="ACV5NJ_002015"
     CDS             503..2731
                     /locus_tag="ACV5NJ_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHY6486070.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            2860..4503
                     /locus_tag="ACV5NJ_002016"
     CDS             2860..4503
                     /locus_tag="ACV5NJ_002016"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HHY6486071.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4576..5121)
                     /locus_tag="ACV5NJ_002017"
     CDS             complement(4576..5121)
                     /locus_tag="ACV5NJ_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HHY6486072.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5144..5629)
                     /locus_tag="ACV5NJ_002018"
     CDS             complement(5144..5629)
                     /locus_tag="ACV5NJ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HHY6486073.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5619..6431)
                     /locus_tag="ACV5NJ_002019"
     CDS             complement(5619..6431)
                     /locus_tag="ACV5NJ_002019"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HHY6486074.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6570..8102)
                     /locus_tag="ACV5NJ_002020"
     CDS             complement(6570..8102)
                     /locus_tag="ACV5NJ_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HHY6486075.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8211..9242)
                     /locus_tag="ACV5NJ_002021"
     CDS             complement(8211..9242)
                     /locus_tag="ACV5NJ_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6486076.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3205 a         1800 c         1868 g         2775 t
ORIGIN      
        1 caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa
       61 gaaatgcatt aaatatggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc
      121 gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac
      181 agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct gcgcaaggaa
      241 gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg
      301 tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa
      361 agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg
      421 gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga
      481 tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc
      541 acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga
      601 atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa
      661 cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc
      721 tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt
      781 tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag aattactaga
      841 agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg
      901 cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga
      961 ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa
     1021 aggctattta ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc
     1081 acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg
     1141 ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt
     1201 aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga
     1261 agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa
     1321 gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt
     1381 agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact
     1441 ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat
     1501 tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa
     1561 agaaaaatgg ggtcatcctc aatctgagca tacaaaagaa agaaccatta gcgccgaaga
     1621 agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa
     1681 atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa
     1741 aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca
     1801 aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca
     1861 tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat
     1921 tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc
     1981 acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga
     2041 gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt
     2101 caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca
     2161 tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga
     2221 tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt
     2281 cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc
     2341 tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac
     2401 tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt
     2461 aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc
     2521 gttgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata
     2581 tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa
     2641 gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca
     2701 aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa cgcacttagc
     2761 agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta aaatagaatc
     2821 ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga acaaaaatgg
     2881 tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag caatggcgat
     2941 ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga tcttggtatc
     3001 ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg ttatgacgta
     3061 agtgactatc aagatataga agagagattc ggaacgatgg aagattttga tttattgcta
     3121 aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa ccatagttcg
     3181 gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta tcgcgattat
     3241 tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc gattttcggc
     3301 ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa
     3361 gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt caatatgatc
     3421 cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc tcatgtaaaa
     3481 aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca aaatgtctca
     3541 ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga tatcatgact
     3601 gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg gaaagacggt
     3661 tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga aaaagaaggg
     3721 caattagatg tccttaaatt gaaacatgca ttaagcgctt ggcaaacttc attggatggt
     3781 atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt
     3841 ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat gatgtatttc
     3901 tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac gaatatgccc
     3961 tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa aaggctgctt
     4021 gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac gagagacaac
     4081 agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac acatgaacct
     4141 tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga atatgaacca
     4201 gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa taaaggtctg
     4261 atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt ctatgaaaga
     4321 tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact tgctgactat
     4381 gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc ctctagcgga
     4441 gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa aacaaacaag
     4501 taaaacttcc tcgttaaagt tcattagttg gactttgcga ggaagttttt cagattgctc
     4561 tgtcaattac tcatttcaat aattcgctct aggaccaccg atatatttag gacgagagcg
     4621 aagggctgtc acgtgagctt gtccaagggt tttcaaacga ggtctgacag gtacttggac
     4681 atgcttgata tgcataccga ttgatgtgtc accgatatcg ataccggctt gtccaactac
     4741 gtgttccact tcgacaggat cattgaactg gtcaaaagca gcaacagaac aagcacctcc
     4801 agcatgtagt gcaggaacaa cagagacaat ttcgaaattt tttgcttctg ccactgttcg
     4861 ttctactact aatgctcggt tcaaatgttc acaaccttgg acagctagcg caatttcttt
     4921 aggatccaac aactctttta acgtacggat gatccattga ccaacttccg cactggaatt
     4981 ttttccaata tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc
     5041 ttttttcaag tgtgcctcag ccaatatgtc attaactatt tcagtcaatt ggtcttttaa
     5101 ttctttttct gacaacttca ttttctaaaa ccttcttttt caattaatat tgcttccgtg
     5161 cttgaatacg ttgaagactt gcagttatag gaaaggcaag tcccattcca atcagatttt
     5221 ggaagatgtt tcctggtata gaagccactc cagccggcca atcatataaa atccaaccgg
     5281 cgataccata gccgatgatc ataactaagc ttcctaataa aagccctaat ccccacatac
     5341 gagtagaaga tttgctgaaa gaaccagcaa catatccttg taacccgtgg atcactaatg
     5401 aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat caatagacca ctggaagcac
     5461 caacaactaa tccacccgtt ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc
     5521 ataatgtcac aaatccattc gtagccggta cagggatcaa aattgttaat gaaagggcaa
     5581 ctgtcaacgc aatcagcata gcatagatcg ttaattgctt agttttcatt tctcattcct
     5641 cctttttgaa taccgccaga ccagacactt ccggtatgat cgttcatcag cacgccatta
     5701 gaaatacaat gggcaacaaa ttttttactt tctgctactg ctttggtaaa atctgcccct
     5761 aacacaagat tcgctgttat tgcagaagaa aaggcacaac cggccccgtt tactgttgta
     5821 gtgtcgattt ttttatgaga aaatacctca taatgatcaa ttgaatagag aagatccaca
     5881 gcttcatctc cagccatcct tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca
     5941 gtgagttctt tagcgagatg gatcatttct gttaacgaag agggaagagc ttgaccagaa
     6001 agtaaagcag cttctgccaa attaggcgta atcatcgtaa caagtggaaa aagttcttcg
     6061 atgagctgcg aaatatacaa ttgattgatc acagcttcag tttctttgaa tgccaaaaca
     6121 gggtctaaaa tgattgggat attttgtttt gtttttatga agtctcgaac aaccatgatt
     6181 gcttcacttg aattcaataa cccaattttg ataccatcta atgaagtcat tttatcaatc
     6241 gaaactaact gtcgtttaag caattccaaa gatatagatt ccagcataaa tcgctcatta
     6301 atatctagtg cccctataga agtcaaggcg cttagtccga atagaccaaa attctcaaaa
     6361 gtttttaaat ctgtttggat tcctccacca gcaaatggat cagaaccgcc aattgtcaag
     6421 attcttttca taacattttc ctccatgaat aagagtataa cgaaattttc aaaaaagaaa
     6481 aacagccaat tggcatattt ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga
     6541 ccaaaatgga tctttctttc cttaaatagt taggactttt gtgattttaa ttctttctcg
     6601 atttgaagcc gttgcacttt cattgtagct gttcgcggga ttgcatcaaa atcacggatg
     6661 atagggacgt tcaagcgagg aagttcatgg acttgctccc accatcgatt ccaatccatt
     6721 tctttatcag gagctaaagc aataatcggt tgtggagaat gatttttatc ccttacaatc
     6781 actacttctg ccaaaaaatc taacgaatct aataaaaagt cttcaatagc taatgtacta
     6841 ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa ataggtggcc atgcttatcc
     6901 ataaaaccgt aatctccgct atcccaccaa tcgccataaa cattttcctg gaaccgtgca
     6961 tcttccttgt aataagtcaa agcccgtcct ttagacagta attgtatatg accatctgtg
     7021 ttttcaggta ggacattccc aactgaatct gtaatccgag cgcttgtcat gtcttccagt
     7081 ccaacgccca tatctcttgc atcacttgtt ttcaaactct ctagtgtatg agccttaaga
     7141 atcattggtc cgcattcact ttggccatat acttgaagaa ataccgcatc ctgtgccatc
     7201 gacgttcgca aaaatgtctt cattgtctgg ttgtttatag catcaaaagt ggagtgatag
     7261 tatcggatac ccgaaaaagc ttctggatgc tccttagcag taaaacgcca ctgaacaaag
     7321 ttattcggat gggtctctag tgcaattggc cggtgagctt ccaacatatg aacaactttt
     7381 gaactttgtg cgtttgctaa aggcatcata ggaaatccca tggacatcaa agaagagata
     7441 ccaatattga atctggaatg cactggagag atgtgaaaag cgattaattt cttttccgaa
     7501 atttttgtga aaactgtttt ttgccatttc gtccgccatc ccatagaatg agcagaatga
     7561 caaataagtt tagggatacc agttgtacca gaagtatggg tcatataaga aatttcattt
     7621 ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt ccattaaata aagaatagga
     7681 atctgttttg attttgttcc atttgaaatt tctctcactc gcttttttgt ctcatcatca
     7741 aataaaatga aagggtcttc taaacgatcg ataaatactt ccattgttgt tgttggtaga
     7801 tgataagaag tcatgacagg cactgcacca aggtaagaca cagaaacagc tagaaggtat
     7861 gtgtcaaaag cggaactctt ataaataata actttgtctc cggatttcac tccaagatga
     7921 gccaattgat aagcccgttt caacacactt tcgcagcatt ttttgtaagt tgtttcaagt
     7981 cctaattctg ggaaagctga aagtggctca tcaaagataa tcggggtctc ggaaaagtta
     8041 gctgcagctt cttgaaaatt cgtaaataag ttcaaagggg aatacgttat ggtctccatc
     8101 attcacactc cattatcttt tgttaagtaa tatacaaatt caagataatc gtaacataat
     8161 cctttatata ataacatagt ttattgtgaa aaaaagatca tacatttgtt ttatatggaa
     8221 gaaacccgaa cgaatgccgc taattcgtga taagaaaaat caaccatctt ttcccagata
     8281 tcttgaaaag aatgggatga gaggtatcga ttaccacata aataatttac cgcgtcagca
     8341 tcatctaaat atagttctgc ataataacga tatgtttttc tgcatagttc tggcgatacg
     8401 tttttatata ctgtaatttt gctcaaaatt tggcgaatag tccgaggaga aatttgctga
     8461 tcatgtgagg aacgaaaaac aaaagaattt tcgtccgtat tttgtgctgt taacttaaaa
     8521 tagttctcca gaaatggcat cgcttctttt ggtattggta ctttattcag tctgttttct
     8581 tttctattca gattaagaat ctgcttggtc aaagatacat ctttacgttt aagttctact
     8641 acttcatgga tctttaatcc gcaatacaga agaatggcag taattgcaag gttcctcggt
     8701 ctattctttt cttcaaagct agcttctctg cttgtttgaa agccttcaat cgggataaac
     8761 aaatgctcaa aatctttgaa aagttctgca tcgatatatc gcggagctgc tttggttgtt
     8821 tccggtaaac ttgtacccaa aagagaaatc tcttcttcaa tcagtccttt ttgatacaaa
     8881 aatcgtaaaa aatgatttaa tgaatattgc ttccttgaaa tcccgtaata gctattttca
     8941 aacaattgct ccgtttcttg gttcgctgta gtttggaacg aacgagtatg tcgatagatt
     9001 ttagctaaga aattctgata atcttgtttt tctaaatgga taatcagctg atcatccact
     9061 tcttcagaaa atacttttct caaataatca gtaaacagat gaaaatcttt tgcgtattca
     9121 tagcgactgc ctaatgaaag ggaagaacga ttttcttcta aaaactgttg aaagacggaa
     9181 gggagcaagt gtgtacgttg ctttaacttt tcttgcagtt gttttctagt tttgctgtcc
     9241 attacaaatt ccctccaata gtgttcattt tctatatttt accgcatttc atcaaatgaa
     9301 gatagacagt ttgtcttgat cttaggaagc aaatttttaa ctaagtacta gctaaaagat
     9361 atgaagtaag aagatacatt tttagttatt ttattagtgc agtcattctt ttgatctttt
     9421 aattgattta tcctcttttt agctatgtgg ttacttcatg agcaatgaaa tggatttggt
     9481 tcgttctaat ggcaggaggc tctttctttt atttccgaaa aattagaaaa cattttttca
     9541 gaatcgattg gctctccatt tattcaataa tggtttgtct aacgatattg ttgggataat
     9601 tcttaaagaa gtattagaaa tagttggtac ttcattactt tacatgaa
//
LOCUS       DBKOIJ010000107         9646 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000107 DBKOIJ010000000
VERSION     DBKOIJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.109"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(116..2350)
                     /locus_tag="ACV5NJ_002022"
     CDS             complement(116..2350)
                     /locus_tag="ACV5NJ_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHY6486077.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2829..3095
                     /locus_tag="ACV5NJ_002023"
     CDS             2829..3095
                     /locus_tag="ACV5NJ_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HHY6486078.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3095..4822
                     /gene="ptsP"
                     /locus_tag="ACV5NJ_002024"
     CDS             3095..4822
                     /gene="ptsP"
                     /locus_tag="ACV5NJ_002024"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HHY6486079.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            4986..6032
                     /locus_tag="ACV5NJ_002025"
     CDS             4986..6032
                     /locus_tag="ACV5NJ_002025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHY6486080.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6049..7272
                     /locus_tag="ACV5NJ_002026"
     CDS             6049..7272
                     /locus_tag="ACV5NJ_002026"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHY6486081.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7361..8248
                     /locus_tag="ACV5NJ_002027"
     CDS             7361..8248
                     /locus_tag="ACV5NJ_002027"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HHY6486082.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8334..8564
                     /locus_tag="ACV5NJ_002028"
     CDS             8334..8564
                     /locus_tag="ACV5NJ_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HHY6486083.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(8621..9586)
                     /locus_tag="ACV5NJ_002029"
     CDS             complement(8621..9586)
                     /locus_tag="ACV5NJ_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HHY6486084.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3103 a         1709 c         1953 g         2881 t
ORIGIN      
        1 ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac ttttctgaaa
       61 aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac tggatttatt
      121 cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta acgatgattt
      181 ttccatcttt atccagtttt gctttcaact catggataga agggtgatca agataaaatt
      241 cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga gcacccatag
      301 atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact tcgatgtgaa
      361 gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg attgtcatca
      421 agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc agaaattctg
      481 gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg cgtgccgcac
      541 caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata atgatgatcg
      601 tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca tctaagattt
      661 gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac aagacaagac
      721 tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat ccgacataac
      781 ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca gacatatcga
      841 aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc gcaagctctg
      901 ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga ttctgtttgt
      961 ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg ttatcttgtc
     1021 cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt tctttttctt
     1081 ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata tctttttcgg
     1141 taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga agtttattga
     1201 tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct aaaacttttt
     1261 gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt tggatcgtca
     1321 agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc aagaagcgat
     1381 cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca gtatatttta
     1441 catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc gtttcttcga
     1501 cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg tctttttcga
     1561 tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct cgagctaaag
     1621 ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca gcaccgacta
     1681 tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc tcttcaatca
     1741 atttttgcat acgttcttca aattgtcctc gtataccagt accttggact aaagatacga
     1801 catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca tctacgattt
     1861 tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca atcaagacag
     1921 gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg tcatcgcgtc
     1981 cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt ccgtattctc
     2041 ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg ttgtttccgc
     2101 caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg cgagacatgg
     2161 aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc atattagtca
     2221 gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa tcgagttgtg
     2281 tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg ccgcagtttt
     2341 gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata ttttacatct
     2401 cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg aggtagaaaa
     2461 ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa aataaaaagg
     2521 gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa tgataaacgt
     2581 caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa aatgtgaaga
     2641 aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg aatgaaaact
     2701 gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca tttgttagga
     2761 ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact taaaggagac
     2821 cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc cacgctcgtc
     2881 cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac ttagagtata
     2941 aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt gtaggtcaag
     3001 gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg gcagcaatcg
     3061 ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa aagggattgc
     3121 cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg atttaacatt
     3181 cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg ctgcattagc
     3241 taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct taggtgaagc
     3301 agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa tggtaggtca
     3361 aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa aagaagtaac
     3421 ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc aagagcgtgc
     3481 tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg ttactttacc
     3541 aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga ctccaagtga
     3601 tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg gtggacgtac
     3661 ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg ggacaaaaga
     3721 aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg aaggcgatgt
     3781 aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg cagattacgc
     3841 agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag ctgacggcaa
     3901 gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg tccataataa
     3961 tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt ctccagactt
     4021 cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta tggaaggaaa
     4081 acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat atctgcaatt
     4141 acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt tatctgaaca
     4201 aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg ttcatggtaa
     4261 tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag caaaagcgat
     4321 ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg atacgatcca
     4381 agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt ttgcaaaaga
     4441 agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg cagcagaccg
     4501 tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt tacgtttgat
     4561 caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt gtggagaaat
     4621 ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg agttctcaat
     4681 gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca cagcgaaaat
     4741 ggctgaactt gctgatcgtg cattgaaaga atgcgatacg atggaagaag tcgttgaact
     4801 tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg taaaagagct
     4861 acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat agacaaaaat
     4921 agggcttaag tttgagtgac agtttgtagg ctatgttata ctatataagt gaagggggac
     4981 gtaaggtgaa ggtattatta tattttgaaa gtgaaaagat gcttgcaaaa tctggaatcg
     5041 ggcgtgcgct tgatcatcaa aaacgtgctc tgacggaagt cggtatctct tatacactag
     5101 atgaaaaaga agattatgac atcctgcaca tcaatacata cggaatcaat agtcataata
     5161 tggtcaataa agccagaaga gaaggaaaaa aagttgtcta tcatgcgcat tctacagagg
     5221 aagatttccg aaattccttt atcggatcga accagctttc tcctatcgta aaaaaatatc
     5281 tggtaggatt gtaccaaaaa gcggactatc tgatcacgcc aacaccttat tcaaaacagt
     5341 tgttggaaag ctatggtatc cgtgtgccga ttcaagctat ttccaatggg atcgacttag
     5401 agaaatatca ccctgatcca atgaaagaac aaaagttcag agaatatttc aaactgtctc
     5461 cggatcaaaa agtaatcatt tgtgtcggtt tgtttttcga acgtaaagga atcattgatt
     5521 tcgtagaaat tgctaagaag atgcccgaat acacatttat ttggtttggt catgttccca
     5581 tgtactcgat tcctcggaac atccggaaaa ttgtaaaaga agatcatcct gaaaatgtcc
     5641 tgtttcctgg ttacattcgt ggagagatca tagaaggtgc gtattcaggt gcagatctat
     5701 tctttttccc ttcttatgaa gagacagaag ggatcgttgt tttagaagct ttagcaagca
     5761 aacaaaacgt acttgttcga gatattccag tgtataatgg ctggttagaa gatagcaaga
     5821 actgctatat ggggaagaca aacgaagaat ttatccgact gatccaacag attaccaatc
     5881 aagaactacc gagtacgaca caagccggtt atcaaacagc aaaagagcga agtatcaata
     5941 aaatcggtga agaacttaaa aatgtatacg aaagtgtact gaatgaaaaa gtctctagca
     6001 aattaaaaaa agtaaatcag gtcaaggact agtttggagg cgaaaaacat gaaaatcggc
     6061 ttttttaccg atacctactt tccgcaagtc agcggagttg ctacatcgat caaaacatta
     6121 aaacaagaat tagaaagaca tgggcatgaa gtctatattt ttacaacgac cgatcctaat
     6181 gcggataagt tggaaaaaga tgtgattcga atgccaagtg taccatttat ttctttcaag
     6241 gatcgaagag ttgttgttag aggcatgtgg tatgcttatt tggttgccaa agaactagaa
     6301 ctagatctta ttcataccca tacagaattc ggagcaggga tacttgggaa gatggtagct
     6361 aagaagttga aaatacctct gatccatact taccatacga tgtatgaaga ctaccttcat
     6421 tatatcggta aaggaaaagt gatcaggcaa tcccatgtaa aatacttgtc tagattattt
     6481 gcaaatcata cgacaggtgt agtttgtcca agtgaacgtg ttattgatac attacgcagc
     6541 tatggtgtaa tagcaccgct tcgcgtgatt cctactggta tcgacattga aaaattcaaa
     6601 cgttctgata tcactcagca ggatatcagc aaattaaggg agtcactagg tcttcaggaa
     6661 aaccatttga tgattctttc tttaagcagg atttcttatg agaaaaatat ccaagccttg
     6721 attaatgggt ttcctcaggt catctccgct tatcctaacg ccagattagt tatcgtagga
     6781 aaaggacctt atgtagaaga gttagaggaa ttgatctcag aactgcagtt agaagaattt
     6841 gtgcagttca caggagaagt ggacaacaat gatgtagcac tttattataa agcggcagat
     6901 tattttgtca gtgcttctac ttcagaaaca caagggctta cttatacaga agcgatggca
     6961 gccggaacac cttgtgtcgt ggaaggaaat gcttatttga accgattgtt cgatcatcct
     7021 tcattaggga aaacttttga aaccgacgaa gactttgcac cagcacttat tgattatatt
     7081 tccagtgaag ttcctaaaga tccaaaagtt ttagaagcta aactttatga tatttcatct
     7141 acacatttcg gcgaagctat gatcgaattt tatcaagaca tggttgttta tcatgaagaa
     7201 gagatgcgaa gaaaagaaga agaagactcg attgaacgaa tcaaaataaa attaaattca
     7261 tttaaaagat aaaattcaaa cattttttat gaaaaaatcc ttgtttctag cgtatactag
     7321 catagaagca aggatttttg atttttagga ggtcgaaaaa atgaaacttg gttttatcgg
     7381 aacaggcgtc atgggaagtg ctgtcgcacg ccatctacta gaagcaggac acgaagtagc
     7441 tgtctataat cggacaaaag caaaagctga tcctttagta acagaaggag ctatctgggc
     7501 agatacacca aaagcggtag cagaacaaag taatattctc tttacaatgg taggttaccc
     7561 caaagatgta gaagaaatct actatggaca gtcggggatt ttttctgcag atatctcagg
     7621 acatatttta gttgatttga ctacaagtac accaagcctg gcagaaaaaa ttgcaaaaac
     7681 agcaaaagaa aaaggcgcag atgcgttaga tgcaccggta tccggtggtg acttaggagc
     7741 aaagaatggt acactgacga tcatggtcgg tggagaagaa gccgtttatg atcaagtact
     7801 gcctttattc aaagaatttg gtacgacatt tacattgcat ggttctgctg ggaaaggtca
     7861 gcatacaaaa atggctaatc agatcatgat tgcaggtacg atgacaggaa tgacggaaat
     7921 gcttgtctat gcacaaaaaa acggacttga tttaaagaaa gtgattgaga cgctttcagg
     7981 tggaagtgca gctaactggt ctttaagcaa ttattctcct cgtattttaa aagaagatta
     8041 cacacctggt ttttttgtca aacattttat caaagattta aagattgcat tagaagaagc
     8101 agaaaaaatg gatcttgtat tgccagcaac tacccaagca ttgaaactat atgaagagtt
     8161 ggcagataaa ggttttgaga atgacggaac ccaagctttg atcaaacttt ggtggcctga
     8221 aggaaaaata ccagaaaaaa aatcataaaa aacgagtttt tttagagaaa atcaatgcaa
     8281 gtaggctatg cttttgatac aatataacag aaataagaaa aggaggacct ccgatgacac
     8341 attcacaatt agtcgcaatc ataaaacgac tggaggctat ggtcgagtca gcagataatg
     8401 aactgcaagt tcgccgtttt gaaaaagaag gcgtagagaa gtgtacagta acttatgata
     8461 aagcgaccga tacatttgaa ttaactgaaa cagatactca tcagcaatac gaatttgata
     8521 atatcgatat cgtagcgatg gaaatctatg acttaatcca ataaatttta ggataattga
     8581 catttgaagt tgaagatgcc taaaagaagc cgagaagcaa ctaatttagt tgcttttcag
     8641 cttctttttt gtttaacagt tcaatttcaa actgttcttt ttctaatttt ttgcggtaac
     8701 tgatcaagat ttgtacagga tcgatttctt cttttgacaa ttgacgtttc aaagataaat
     8761 gagcttcttt cttttcataa tagaagtcag gagaaacgtc accagaggta atcattgttt
     8821 ctgcaagttg accataacaa acgaggtagt gctttttctg actctcatct gctgctaatg
     8881 gcatgctgaa ggtgagcgat ggtgttctta aaaactgctt ttgttcctgg atccgtctaa
     8941 taaaataagt aactaattcg atatcttttt gaagcatacc ggctttttcg aaattcaact
     9001 gatagatgaa atgttgttgt ctttttttca agtaagtcag tagcttttta tttcggcctt
     9061 gaaacagccc ttggagttcc ttttttttct gttcaaaagt catttcctgt aggtcaggaa
     9121 gctggactcg caaagctaac aaactaattt cagacaacca aggcatttga taagtttctt
     9181 ccattatacg caaaatagac ggcatttttt tatagagacg aaaaggcccg tatgttcgag
     9241 ccgtaggagt atctgttaat acaaatccct tattggtaat atgtacataa ttataattac
     9301 tgaaataatt catttgtcgg ttataaaaag gacggtaatg ttggatcaat tgacactcga
     9361 gcaataaagc atctaattcc gtgtctacta cgacgaaatc aaaatctgtt atatgatgaa
     9421 tcatacgtaa tacttttttt gaatgctgtt tattctgatg aaaatagctg cttactctat
     9481 ttttgagccg ttttgcttta ccaacataga tgaccttacc atctcggtct ttcataagat
     9541 agacacccgg cagcatgggg agagttttca ttttttcttt taacatagga tctcctttta
     9601 cgctttgaat aaaaagacta taacaaattt tgaaataatg gacaag
//
LOCUS       DBKOIJ010000108         9594 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000108 DBKOIJ010000000
VERSION     DBKOIJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.110"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..641)
                     /locus_tag="ACV5NJ_002030"
     CDS             complement(<1..641)
                     /locus_tag="ACV5NJ_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HHY6486085.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACV5NJ_002031"
     CDS             complement(643..1407)
                     /locus_tag="ACV5NJ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HHY6486086.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACV5NJ_002032"
     CDS             complement(1415..2092)
                     /locus_tag="ACV5NJ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HHY6486087.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACV5NJ_002033"
     CDS             complement(2129..3514)
                     /locus_tag="ACV5NJ_002033"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HHY6486088.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3723..3809)
                     /gene="ffs"
                     /locus_tag="ACV5NJ_002034"
     ncRNA           complement(3723..3809)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACV5NJ_002034"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACV5NJ_002035"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACV5NJ_002035"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HHY6486089.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACV5NJ_002036"
     CDS             4453..5310
                     /locus_tag="ACV5NJ_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486090.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACV5NJ_002037"
     CDS             complement(5391..5726)
                     /locus_tag="ACV5NJ_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486091.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACV5NJ_002038"
     CDS             complement(5793..7163)
                     /locus_tag="ACV5NJ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HHY6486092.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACV5NJ_002039"
     CDS             complement(7241..7777)
                     /locus_tag="ACV5NJ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6486093.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACV5NJ_002040"
     CDS             8086..9567
                     /locus_tag="ACV5NJ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486094.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1970 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt attc
//
LOCUS       DBKOIJ010000109         9432 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000109 DBKOIJ010000000
VERSION     DBKOIJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.111"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..680
                     /locus_tag="ACV5NJ_002041"
     CDS             <1..680
                     /locus_tag="ACV5NJ_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6486095.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACV5NJ_002042"
     CDS             800..1000
                     /locus_tag="ACV5NJ_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHY6486096.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACV5NJ_002043"
     CDS             1076..1273
                     /locus_tag="ACV5NJ_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486097.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACV5NJ_002044"
     CDS             complement(1570..3117)
                     /locus_tag="ACV5NJ_002044"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HHY6486098.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACV5NJ_002045"
     CDS             complement(3201..4004)
                     /locus_tag="ACV5NJ_002045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HHY6486099.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACV5NJ_002046"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACV5NJ_002046"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HHY6486100.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACV5NJ_002047"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACV5NJ_002047"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HHY6486101.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACV5NJ_002048"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACV5NJ_002048"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HHY6486102.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACV5NJ_002049"
     CDS             complement(6389..7318)
                     /locus_tag="ACV5NJ_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HHY6486103.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACV5NJ_002050"
     CDS             7513..8196
                     /locus_tag="ACV5NJ_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HHY6486104.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACV5NJ_002051"
     CDS             8357..9400
                     /locus_tag="ACV5NJ_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HHY6486105.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DBKOIJ010000110         9387 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000110 DBKOIJ010000000
VERSION     DBKOIJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.112"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(212..406)
                     /locus_tag="ACV5NJ_002052"
     CDS             complement(212..406)
                     /locus_tag="ACV5NJ_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486106.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(419..601)
                     /locus_tag="ACV5NJ_002053"
     CDS             complement(419..601)
                     /locus_tag="ACV5NJ_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486107.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(598..738)
                     /locus_tag="ACV5NJ_002054"
     CDS             complement(598..738)
                     /locus_tag="ACV5NJ_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486108.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(1228..1593)
                     /locus_tag="ACV5NJ_002055"
     CDS             complement(1228..1593)
                     /locus_tag="ACV5NJ_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486109.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(1633..1935)
                     /locus_tag="ACV5NJ_002056"
     CDS             complement(1633..1935)
                     /locus_tag="ACV5NJ_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HHY6486110.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(1935..2234)
                     /locus_tag="ACV5NJ_002057"
     CDS             complement(1935..2234)
                     /locus_tag="ACV5NJ_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486111.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(2227..2391)
                     /locus_tag="ACV5NJ_002058"
     CDS             complement(2227..2391)
                     /locus_tag="ACV5NJ_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486112.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(2397..2666)
                     /locus_tag="ACV5NJ_002059"
     CDS             complement(2397..2666)
                     /locus_tag="ACV5NJ_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486113.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(2678..3427)
                     /locus_tag="ACV5NJ_002060"
     CDS             complement(2678..3427)
                     /locus_tag="ACV5NJ_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486114.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(3424..4116)
                     /locus_tag="ACV5NJ_002061"
     CDS             complement(3424..4116)
                     /locus_tag="ACV5NJ_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HHY6486115.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(4122..4793)
                     /locus_tag="ACV5NJ_002062"
     CDS             complement(4122..4793)
                     /locus_tag="ACV5NJ_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HHY6486116.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(4786..5127)
                     /locus_tag="ACV5NJ_002063"
     CDS             complement(4786..5127)
                     /locus_tag="ACV5NJ_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486117.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(5305..6003)
                     /locus_tag="ACV5NJ_002064"
     CDS             complement(5305..6003)
                     /locus_tag="ACV5NJ_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HHY6486118.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            6090..6560
                     /locus_tag="ACV5NJ_002065"
     CDS             6090..6560
                     /locus_tag="ACV5NJ_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486119.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(6603..6782)
                     /locus_tag="ACV5NJ_002066"
     CDS             complement(6603..6782)
                     /locus_tag="ACV5NJ_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486120.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6769..7107)
                     /locus_tag="ACV5NJ_002067"
     CDS             complement(6769..7107)
                     /locus_tag="ACV5NJ_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HHY6486121.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(7123..7326)
                     /locus_tag="ACV5NJ_002068"
     CDS             complement(7123..7326)
                     /locus_tag="ACV5NJ_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486122.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(7341..8117)
                     /locus_tag="ACV5NJ_002069"
     CDS             complement(7341..8117)
                     /locus_tag="ACV5NJ_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HHY6486123.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            8416..8736
                     /locus_tag="ACV5NJ_002070"
     CDS             8416..8736
                     /locus_tag="ACV5NJ_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486124.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            8754..9185
                     /locus_tag="ACV5NJ_002071"
     CDS             8754..9185
                     /locus_tag="ACV5NJ_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HHY6486125.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         2652 a         1862 c         1450 g         3423 t
ORIGIN      
        1 aacgttttaa agaatcagga ttagaacaat taaaaaaaga taattctcca gaattatttt
       61 ctaattttac cgaagacgac attcctactc ttaaacaatt aaaatattat tctgattatt
      121 ggacgcaaac tcgtgagaat gaattaaatt tagaaaaact cattattaaa aagaaatagc
      181 aactccagcc cacacggctg gttttttagt ttcaacttcc ctccttacgg gtcaaaatca
      241 tcggttccgg cataatctga tcatccttta gcaacaaatc aggatatttt ttccttagct
      301 tcttattgtt cgtgtaagtt ttcaatagcc attggttggc ttcgcttttg ctttctccag
      361 aaaaataaat tttccctgtt tggatgcctg caagttgaat catcattttc acctcaaatc
      421 aaattttttc tgctagatgg agcaaaagca atccgacatc aagaatcttt tgacccatta
      481 tttcagctac tttgctcggc ttaactcctt gagaaaagag caagcgtgct tgctctatct
      541 cttcgtgagt ccaaataaat ttggcttcct caaggataat tactggatca gttgctctca
      601 ttgttcctct ggaaaaatga tgtcattctt atgcttggac caggtatctg catatggaac
      661 aaagtattgt ctagctaatt cgatttgatc aattaaagca gtttctgata attcatggtc
      721 tgcagcaatt tcattcatac tatcgccttc atctaaacag ataagaccgt tgacgttatc
      781 gatttttaca tttgtgactg cttgactttc tccacgctca taatattcta cagttgatac
      841 tgatacatca gcttcggagt ttaattgcag aatctgttac tgccgggata atttaatatt
      901 cttttcataa ttcacctttt ttcctactga catcgaatat ttttcatgtt atgattagcc
      961 gtgaaaggag agctatatta tgtgtttaca catgatgtcc ttagctcatt cggttctttg
     1021 gaagcttttt tggaaataac tctcaggttc ttgacgcaaa tctcatttat tctttgaaac
     1081 aaaaaacttc cattttccga gagctttgga tcacttcaaa agagatttgc atttatattt
     1141 ctgtgacctt gagtcgcgta aagaacctta cagcaatggc ggcaattcca ctttcaggaa
     1201 accgcctttt ttcattgata ttggtggtta gcggactacc cattttcttt cggcagtgcc
     1261 aaaccacaaa tgatctgtgt tgatgtttag gtagttgtat accagataag ctaaatccaa
     1321 agtgcctgca tcttcaagac gacaatattc caaaaattct cccatagctg gacataaaaa
     1381 tcctggagct atcatatccc aacagttaat agtgcaaaga aattcgttta gcttctgaac
     1441 gttttctttg gtttcacgaa ttacaacata ctccaattct tctccgctgg cagagcattc
     1501 caaaacttca aattgattaa gaaatggtag aacatatgct tcaaacatca acattccatc
     1561 tttctgtttt tgcacttgtt ttgcatcaat catgtctatc ctcctattca gttatttctt
     1621 ccattaatgg aattatttca ggtcactgga cttaacgaaa acaccattga ccatctttcc
     1681 agtccgacct ttgatttcgt cataagcaaa atccaagcac tcgtataaat ccatatcatt
     1741 ttgcattgcc aagatgatca aggttactac tacatcgcca attccgtctc ttaaaccatg
     1801 ttcatctttt ctagctagag aagcggcaac ttccccgatt tcttcgaccg tttttaacat
     1861 ttgcttgctg aaatccgctt gatccaatcc cttatctttt gcccactgct ctactttcgc
     1921 gattagttcg tccattattt ctcctccaca tacctaaact gtcttccttt tgaatcaatc
     1981 cataagctcc tagctctatc ccaaataata tttttgctca gaccagtaat ttcagataac
     2041 tgtttagcgg tacccgttac tagaattcga tcaccatgcc agattgcaat ctttctcggc
     2101 gttctccgtt tgggcttttc agtccacatt gatttaccga gcttttggac ttctgcaact
     2161 atttttttat cttcctgcca atcttctgaa taagtcaatt cgataattcg ctgcattgcc
     2221 gctttcttat ccatcccgac attctccttt cagtaatttg agtacttgat caagtgcgct
     2281 ctcacgtccg ccatgaaatg tgttgagcca cttgtcttcg tacgacacac tttgtcttaa
     2341 agcttcttga tgcattaatt cgatctgtgc tgtaaatgtc tttaggtcca tcttgtttac
     2401 acctgctcaa gttcattaag atgtttttgc aagcctttaa cacaatcaat gaatagtaat
     2461 ttggtatatg ctaaatttct taattgcgtt ccatcgatat agagtgcgaa atagtatctg
     2521 agtttactcc aactcgaacg atcattctta attccttcaa ttccagcttc ttcgagttga
     2581 tcatatacgt ctctcaggac gtctaattcc tcgccctttt tataattcgc tatttcatta
     2641 attagttcta gataatcgat cttcattttt ccacctctta gaatggtgct tttgattgtc
     2701 tattagctcg ttctagcgct tttttcttga gataggcttc ttggtcgatt gcccattcag
     2761 gaagcttctc tcgtcttcct gtgcgcttgt atccactgct tgcgttctta ggttcacttt
     2821 tttctttcct tgcccaactt cgaatagttg ccaaatagtt tttataagtc ttaccagatg
     2881 attcacaata ctctgacagt cgttcgattc gctcttggta atcattaggg aattctgttt
     2941 tgagtttctc catctgctca tctgacaaaa gaacattttt atactctccg tatttatgac
     3001 ggacgggctt agccttcgat tttttcgaag gcggtaactc tcttatatat tcttttgtat
     3061 tattaaatgt attattaata gatgtattat tatctttgac tttttcgtca ataggggtat
     3121 tgcgtttttc gtcaataggg tattgattaa ttcgtaggta cctattgatt atttgattgg
     3181 taccctcttt gtaaatgatt tcccgattca agtatccaaa cttaatcaaa tcacttaccc
     3241 atcgcgatat ggtctcttta ttcacaccat ataaatctgc aaagtactca ttgcctgccc
     3301 aacaaaagcc tctttcatta cacaaggccg ttatctctcc gtataacaac ttagtatttg
     3361 gtttaagtct tttgtcgtac cttacgttgg ctggtataat cgcataataa cttcgatgtt
     3421 ctgtcatttt tcccctccaa tatttaactt tttgattgtt ttctggttta atttgatccc
     3481 tttgatttga tatttatttt tgaaattaat cacacctatt ttgtgcttct ctgtgtgatg
     3541 gattctgcag agtgctgcaa atgtgtactc tgcatgatca acttctttgc gctttcgtct
     3601 tcctagcgct ttgtcaaagt gatcgatgtc agctcctgtt ttgccacaga tgcagcagac
     3661 tctttttgtg atgcatttgt agaagtaata ttcttgattc gcaggtaaaa tctcatagcc
     3721 ttctttgaaa ggaatatgat gttcaaagat gaaatctaag atgatatttg ctaagacatt
     3781 ggcatcactc acagttgtat tcgattcgtc tttcaggctt attttgcgcc ctgtgacacc
     3841 ttcaaaacga aagtagaaga attccttcca gaagtccgtt ggcatgcctg tatcgataaa
     3901 aatatcgcct atcagcgcat agatgaagtt tcgttgctgt acggtgaaac gtctaggatc
     3961 aataaaacga atttcaatga ctcgatcacc atcgtagccg tcatacatcg tctttagtcg
     4021 atcaatgttc acttcctcat tgatggttgc gcttatgtct tttcctttga actttttcag
     4081 aaccgctgaa tatgaatcga ttaatggttt aaacactcat atcacttctt atctaattct
     4141 tttctcttag ctgctattgc tcgctccatc aaggcacatt gctcatagct taactgttca
     4201 atagtttcaa cgttatcagc taagagccct aatttatctg tctgctcatt aacatattcg
     4261 attaaggttt tggtcatatc tttacccatc tgctcattga aagcttctag aatcgtctct
     4321 agcatgttta atttctttgt atcgattcta ggtggtgttg gaatatcttc cccttgaaat
     4381 acatataatc ccagtccgtg tagagccaat gctttcacaa agcatcgctt caatgagtta
     4441 ttgatttgca ttgcatttgg tttaacaact ggttggtttc gataatctaa aacaggaaat
     4501 aattcggttt ccgtgtgtcc tttaaccgtt actgagacag atacataagt cccagtttca
     4561 tccataagaa aaggtttata ttcctcaaca agaaagtctt gatgagttcc agaaacaacc
     4621 ctgtagtgtt tgtactcatt aatagttacc gttgcctgtg gatcattctt tttcataatc
     4681 tcccacgcgt gagcccaaga taaataatca aaatttcctt ttttcttgag aattttattt
     4741 aacttacgac taaaaagttt ttcaaagttc gttgtccctt tgatttcact catcaaattc
     4801 tgcctccatt tcagcaatgt atttcttacc tggtccgtaa taagagatat caatcaagtt
     4861 atctctgtcg tactcttcta gcgcatcaat caagccatct tcgatgacgt aaatatattc
     4921 aggtttattc gaatgcttcg atagatggat aagataaaca tgatcccaaa tagttacatg
     4981 gttgcctaaa tcatcttgat cccaagctag ttcttcattc gtcaaaagat ttcgtctgat
     5041 ttttcgacca cttgtttcct caattttcgg cttgccccaa tcaggatcaa tcaaatattg
     5101 atctagagtg gaaagttctt tttccatatg ctaaaatctc cttatgatgt gttttctttg
     5161 tgactctttg cttgccggcg gagtcacttt tttatttgtt gccatgcttt ttgcttgtca
     5221 atatgttgtt ggcttaggat gattggttta tagtatttcc accagcaatt agcaattgcc
     5281 gtccctattc ttagcgcttc agctctattc attgtcatca ccaaaaagtc tctgttgtct
     5341 gttcagttga tcaatttcca tacggatcgc agtttctggc aaccacattt caataaatga
     5401 aacagcatca tcgaatctct tacgaggtaa ctcgccatat cttgggattg aaaaggtacg
     5461 tttaaattca gaccaaaatt ttgagaatac ttttttgctg atttcttcat aagctcggct
     5521 ttcttttccc cctagaactc ccataacttt catatttcct ttttgcttaa tttcaaactc
     5581 ttgttgtccg ctaattcgca tagtatcttt aagcatggaa acatcttttt taacatcttt
     5641 catttcttct aattgataga tcatcatatc ttcaattgtt tgaggaacag tattcttgcg
     5701 aataacatct tccatttcgt tgaatgcttc aatgtatttt tgtttgaagt agatagcttt
     5761 ctttcctgta aaccccatag ccagcaagaa aaatccatct ctactaatga agaaaactcg
     5821 tcgatttctg ccatatgaat ctggttcatt accttctaca aacatctgtc caaaattgga
     5881 cacatctttt tttagtgcat caatatctct taaaacatgt tgatgttttt tctcgaagct
     5941 ttctgccact tgtaagctcg tagtcacagc ttctttattt ttcaaaatta ctaattcttg
     6001 cattatttct tctctccttt ttggtataat ttagttaaaa actggtggtg tttattttga
     6061 atttttttca cttaacaatt tttcaatgga tgtcaataat aagttttata ttgagtacgt
     6121 ttttaacagg gataaaaata aaaggaatgc gccctcaact agaagttgaa cttaatgctt
     6181 cttacttcgc tgcagatatg atttatacaa aagtaatcat ttctaactat tcaacagaac
     6241 cagctatgtt agttaattta gaactttcat ccgctaactt aaaccgcaga tggtcagcta
     6301 ctccatttaa gaaattaatt gctaaaggag gatcagtcga tgataataga atatactctg
     6361 aagctgtacc gctaaatatt cctcctaaaa gtgctatatc ttgttacctt gcatttgaag
     6421 tagggaaaat taattttaat aaactcctta atcaccaaac aaaaatgata tttactctaa
     6481 atagaaccca aatccacaaa attgttgata tcaaaaatac aaacttccca atagagaaat
     6541 tagtgaaaga attaaactga gtaatagtaa tatacattct agtttctctt ttctattcat
     6601 ttttagtcag ccccctcggt tggctttttc gctctgtact cagcttcagc ttcatcaagc
     6661 cccataaaaa tccaaaccat gtaaacaatc gttcctatca acgcttgcct gcttccccaa
     6721 agtcctaaag cgtagatgat tagcggtgcg ctgaacacta gcgctctatt aaattttccc
     6781 atccgcttgc ctccttaaga tttccgaaaa atttgtttct aaaaattcaa gtgttttact
     6841 tcttaaaaat agatatgtgt ctcttccttc aactggataa tagacaaatc catttttgtt
     6901 tttctcgata tcgataatat ttctatatct tgggttcttt aaaactctag aagtaaacca
     6961 atcatatttt ctgttaatcc gttctagcac ttctggcaac gtcatccatc taccagtatc
     7021 atcagctttt tttaactcct cataatccac ttgggagata attacatagc cttctggaat
     7081 tgggattttt gcttctagat attgcattag ctgttcctcc ctttacaatt cgtacatagt
     7141 gataatcgaa tctataattc tgtttgcttc tgcagaagtc tttttaccgt ttaaaattaa
     7201 agataagtag cttttgctaa ttccaaatct ttcagcaagc atggtgtaag ttaagaactt
     7261 tgaactttcg acatattctt tgattttttc tctatctcgt tgagtgattt ctgcaatgtc
     7321 agtcatacta aaactccttt ctaaccaatt tcctctaaat ccatttgagg gtaatatcct
     7381 tcttttttta gtaattcgta gataaataga cgccctttct gtgtccattt ggtattcatt
     7441 acaattttag tgccaccatc ggctttcggg atctcagttg tatgagattt tgtgtatcct
     7501 tggttcatgt gttttttgca caataaccat tggttaccga cttttttctg aatacctagt
     7561 ttatgaagta atttattcat ctgttgtgga gacatcccat aatctgctgc aatctgacta
     7621 attgttactg aatctgtaga agataatatg ctatctaaat aggagatttt aggttcgtac
     7681 tctgcaatct tttgttctgc gattaatctt ccagttcttt cttcttttag ttgagttgct
     7741 aattgaatga ttgtatctgg attaagcaaa gcttcttcta ctttttctgg agttagataa
     7801 cctccatgtt ttctaattgc tggcaacact tcacttgtta cccaacgttt aaattttttg
     7861 gcagagggaa gttttgattt taagattaaa ctgtacaaac cagattcgtt gatgattgtc
     7921 atctctcttg attgacctga ggtcgtgatt cgcgacccca tcttatcttc caaatctaca
     7981 tgccgcgaca aagcatcttt agtgtttgaa taacccaaaa cactcgcaac gtctttgcct
     8041 acaaaatatg gttcatcatt tactaaaatt gtccgaactt cgttttgttc gaaattaaaa
     8101 atttgcggtg tgttcatatt tctcattcct ttcttttata ttcgtaaaca aatttaacaa
     8161 cttttttcta aattcaattg acagtatctg agtaatactc tatactaaga gcataattaa
     8221 ataagacata aaaacattga ttttaaagct ttcttggcgg tctgcattta catatcaata
     8281 gtgttttttg ttgtcttttt agttgttaaa tctgtttaca agatatagta tagctcatta
     8341 ctcagaatta gtcaaattat ttctgcgtat ttttctaaac tttttttgta aacatttaga
     8401 aaggttgttt tatcgatgaa tacctatgaa ataataaaag acttggcgaa gaggaaaaag
     8461 atatctatac ggcagttaga aattaatttt ggatattcta acggttatct agcaaagtgg
     8521 aaaaataata ctcctaattc agatgaatta agtcggattg ctgactattt cgatgtctct
     8581 gtagactacc ttctaggtag agaagaaaga gaaaccccta aacatgtgga tttatcagaa
     8641 gacgatactg ttttttcttt tgatggaaaa gaaatatcta aggagacaat gcgtaaagcg
     8701 attgcaattg ctaaagcttt agaggaaaat gaatagttgg agtgatggtt tgtatgtatt
     8761 tgaaattgaa agaaatgttg agcgaataca atttgaaagt agtttatatg gagatgaaag
     8821 agcctggttt ctactatcca aaacccagaa tcattttttt gaatgaaaat ctatacggtg
     8881 aaactgcaga agcctttcat ttatctcatg aacttgcaca tttcagcgcc tctcattttg
     8941 aattttcagt tttgtacgat acttctacaa cttttcactc aaaatttgaa acagaagcag
     9001 ataaaatagc tattctaatt ttattaaata tctatattga gaacgaactg actgatgaat
     9061 ctcagtttaa tctcgaaaaa tttatggagt attattctat tcagaataag ctcagataca
     9121 cttgttatgc tgtctgccaa tgctatttta agaaaaaata ttcttacgca aggcaatatg
     9181 tatgaataca tcaagaatta ttaaaaaaac cgatttcagc aaaacattaa aaaagcccgt
     9241 gctgcaacac gggctctttc ctcatatatg agcttctaca aaaaaaatca tatcacagaa
     9301 atgaggaaga aaaaatggaa aaagagattt taataaaatc tccaggaaaa accttgatta
     9361 aagtaactga tgattctatt tcaatta
//
LOCUS       DBKOIJ010000111         9353 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000111 DBKOIJ010000000
VERSION     DBKOIJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.113"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            438..1241
                     /locus_tag="ACV5NJ_002072"
     CDS             438..1241
                     /locus_tag="ACV5NJ_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHY6486126.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1826..2281
                     /locus_tag="ACV5NJ_002073"
     CDS             1826..2281
                     /locus_tag="ACV5NJ_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486127.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2400..3458
                     /locus_tag="ACV5NJ_002074"
     CDS             2400..3458
                     /locus_tag="ACV5NJ_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HHY6486128.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3500..5449
                     /locus_tag="ACV5NJ_002075"
     CDS             3500..5449
                     /locus_tag="ACV5NJ_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHY6486129.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5452..9093
                     /locus_tag="ACV5NJ_002076"
     CDS             5452..9093
                     /locus_tag="ACV5NJ_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HHY6486130.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3375 a         1337 c         1929 g         2712 t
ORIGIN      
        1 ttttttattt ttaagatttt gaaataagag aggagtaatc ctcctttatt aaatatctgt
       61 taatatatct gttaaatatc tgttaatata tctgttaggg gaggcttaat ctcttgggag
      121 agtaagtatt aaaggggtat aaaggtaaca cttttggaca gtaaaggtaa caaaagtgga
      181 cagtaagggt aacagttttg gacagcaaag gtaacaaaag tggacatttg aggtatcaca
      241 ctaggataag acgttacctt cggttttctt gatttggaag gcgaaaaaat agaaaattag
      301 attagtaaca aatctttgtt gactgttacc tttagttgtg atttaattta ggtaacaagc
      361 agaaaaaatt gtttccttta attagtacca gattaacatg attaaaggag cgcaaaaaga
      421 agattggaga gtaatcaatg tctcaagaag ttaccgaaaa gattattgat agtgatgtag
      481 atttagtatc agaacaaaca aatgcaattg tgcgttataa caatggtctg aatacaacag
      541 ctttgcgagg atttaagcct gttgaaatgg atgtgttttg ggctatttta tcaaaaatga
      601 aacgtcaagg aacagcagaa gtaacttttg atttcaacct ctttcgttat ttggtcaaat
      661 acgacagaag agaaaaggac agtttttatt ctgtgttaaa agatttgagt gacaaactag
      721 gtaatttaac ctaccgtttt gaagattcag acgtctatga acagctgtgg ctttttcaaa
      781 aattccggat agataaacag aaggaaacag ttacaattaa ggcaaatgag gaatttgaat
      841 ttatcttaaa ctcaattggc aaaaacttta ctcgttttga acttgataat tttacaaaac
      901 taagaagtag ctacacaaaa gaattgtatc gtcatctcat gcaatacaga aataaagagt
      961 ggcgaaatgg ttattgggta gtcaaagttg aagattttag aagagcatta agtatacctt
     1021 caagttatag aatgacaaat attgataaaa gaatactaga gcaggcgaaa gaagaatttt
     1081 tagctcctga tgaaaacgga atacaggtat ttgaaacttt tgattatgaa aaaatctatg
     1141 caaagaaagg aagaagagtt gatcggctca gattcacttt tagtgagcca gaatcaaact
     1201 tgccaaccat ttcaatgcat aattggttag aagaagattg agaagaagca ggttgtaatt
     1261 ttaatacaac ctgcttcttt aaaaaagtat gggtaacact tttggacaat aaatgcaaga
     1321 agtttttttg taagcaagtt tcagtctaaa taatatttga aagaatggct aatctataca
     1381 aattttaaca actatgggta acacttttgg acaagtcaag aaaaaaaaca atttttctta
     1441 atttgttgat cgactaaagg taacactttt ggacaacaca gaataaaaag tccggtgata
     1501 tcagttttgg taggaaacat cttaatatct ttgtgagatt gaaggtaaca tttttggaca
     1561 agtcaactat ttgttaaaag ttaatataga gtaattagat aagttttttc ttttcgtctg
     1621 ataaatggat aatattagtt attttattgc taattagtct ttcatgatga ctgaaatagc
     1681 taaaataaat atctcgttgg ataattgctg atactttaag ggatatctaa atttgctgat
     1741 tgatttattt atgttattat gatattataa taacataaat gtgttacgcc cgaacctcat
     1801 tttaaaaaaa tgaaggggtg agacaatgaa tgctattaat tggcatgaat ttgttaacta
     1861 tatggagagc ttactagatg atctaaaaag ttttagtatt gctgatgcag taaagcatgg
     1921 gttaccagtt agtacaacga tgaaattacg aggattgaaa attccagatg aaattattaa
     1981 gctgagaact aaaacaatta ttgaaaccgc aaaagtcgtt gacaatatga accatgaact
     2041 aaaaggcaag aaaacaactt acgagaaaga ttttgaacgt tttttaaaat ctgttattgg
     2101 aaaatatcta ggtaaaaaat ctttcagtga attgagagat gaagagattg tacctactcc
     2161 aatggttagc aaaatccgaa aaaacaaagc cgaggggaat tactatcttg gtacaatagc
     2221 aaaaatcatt gattatttgg aaaataaaga gcaaaagaaa ggaacgaaga aaggaagata
     2281 atttttgtgt taaatattaa tggggaagaa tataccgaaa tgattcaaaa tcccgaacct
     2341 agaattaccg aaaaaacgtc tgattgtttg ctcttttatg aaagggggca gacaaactaa
     2401 tggaaaaaaa cgtcaattca acatctacca aagatcaata cagtattatt ggagggttgc
     2461 ttggtgataa ggataagaga acacgtcacg gattacgcat tatgcttgca acttttctag
     2521 gagtgctttt tttattaaca ggcttgggta gctacattta tcaattagga aactataatt
     2581 attatctcgt gaacaataca acacctatag gctcttcatt ggagtttgct cgatcaggag
     2641 cagagatcac tatttcagat gtgtggacag atcaaaacag agatgtaaca gtagtaaaac
     2701 tcggttactc gcaaaacgct agaaagttgt tatctacgca aggaaaaaac tacaacttat
     2761 atatggcaac gaaagaaaaa gatcgtccta aaaagcttga aatgagctat gggatgttaa
     2821 gtactgatgg ggatgctttc ttattcctaa aaggtaaatt agatgaaaga gcttatcaag
     2881 tttttattgc aaaccaagta tcactaattt ctggtgatga tacagctgac gatgatacca
     2941 aagtcaatgt gaatgaagca gattcagtta caaaagcact ttctcaatat tctatgaatg
     3001 atgttaataa tgaaggagta atttcttttg ggaaaaaagc aaaacctgtt aatactcctg
     3061 ataatatcaa tttcagaata aatccatatt cagattcaac taatgtctat caaggaagct
     3121 ttcttaccaa agatggcgat attgattatt caaaagttgt agcagttaca agtaagaata
     3181 atgttgtaag taagttagaa gatcagttaa atgcttcaaa tactaacatc aagaaaattg
     3241 aagctacaaa agctgagtat ctggaacgtt tacaacaaaa tcctgacgat aaagattcgc
     3301 aagaaagtat tcgtcaacta gacagtcgaa tggagaccga aattaaagca atcaatgata
     3361 cacaaaaatt gattgatcaa tacaaaaatg ctgactttaa caaagattct tttggagaca
     3421 tgaagacgac atttaagtac ctaactctga ctgactagaa gtaaattatt cttattttag
     3481 aggaagtgaa cacaaacaga tggaaaatag taccgagttt tttaagaata ttcaaactga
     3541 atttaatcat gctctcacac caatgaaacc accgaaacat aagttagtgg gacgtgatga
     3601 cgaactccat gagttgtcac tgatcttgaa tagaccggaa acaccgcaag ctatcttgtt
     3661 aggtgaagcc ggaacaggta aaacagcact tgcaagtgaa tgggctgaaa gacagcgaca
     3721 agatggaaag aaagtacaag tatacaaatt atcaatcgga caattgcgtg ttgggggaga
     3781 tgatgtcctc caaacacgtt tagaaacatt aattccaaga ttgaaagaat atgagcgaat
     3841 tttaaaatct gaggacaaag aaaatgaatt tgtcctcttc attgacgagt ttcataaagt
     3901 tgttagctct tttggtagtg gcaataaaat tgggggtgat tcgctaaaag aaccgttagc
     3961 aaatgaatca cttcaattga ttaccgctac tacaagaaaa gaatatgaaa actatatcaa
     4021 aagagatgat gccttaggga gacggttaca accgttagaa ttgaatgaac taaaaagcga
     4081 tgttgtcgtc gatattttga gacaatggct aaatacatcc tctgttaaag atgatcctca
     4141 ggcttctttt aaattatcgc aaaggattcc tgatgaatta cttcaagaaa tcgtacaagc
     4201 aaacaagctt tatcgtgagg atttgtccga accggctaaa tcaattgccg taatggagga
     4261 tttgattgca aaagatcgag tagacggaaa tgcttataat cgagaaagtc tattgcaaat
     4321 atttggtaag tataatattc agctagattc tgattttgat atggaaaaac ttcaagagga
     4381 attaaagtcc ataaaagggc aaccgttagc agtttatact ataacaatgt tgtttaaacg
     4441 tatgaagctt ggatttgatc gttttaaagg tcgaaagcaa ccaaaagcaa ctgctctgtt
     4501 tacaggatca acaggggtcg gtaaaactgc aatggctaaa gccgtggcaa gaggacttta
     4561 tggcgatgaa aataaagttc tcttagttaa tatgccggac tacaaaacaa aagatgatgc
     4621 aatgcgtttt agacgactca taggtactca tgtaaggcat gatccttctg cgattattgt
     4681 gtgtgatgaa tatgagaaag ctggtgtaga aacactcgat gcctttttat caattttaga
     4741 tgaaggtagg ataagtttta ctgaaagagg atgggatgat cgagaagttc acgacactgt
     4801 ttcattgtca aatacaattt tatttgcgac aacaaatgca ggtgcggaga tgttcgattc
     4861 gttaaacaga tatagctcac tatctcgtgt tacaaacaag gaaaattcta atgaatatag
     4921 cactgaacta aaaagagaat accgaaaagt cgcatcaggg atcaaacaag ccttgattgg
     4981 tgaaggtcta aagcctgaag ttattaaccg atttggacga atagttccat tttgggcttt
     5041 atccgaacaa actcttctgg aaattgctta taatgaaatg ttgaagagtg ctgaattagt
     5101 acgaaaacat ttaaatatag atttacgtct tccacctagg aaagaaagag tatatggtca
     5161 aaatttttat gcagacgatc tctccatgca cttagttttt gacatggctt cacaagagga
     5221 ttctaaatca gggggtgcaa ggaatatctt gcatttagtt gaatctgagt ttgaagaagc
     5281 gattactgaa gcaataatta cttatccaaa cgcaaaaata ttcgatgtta agttgagtga
     5341 agccggagcg tttgaaggaa aaggtgattc taatgctgaa attattgcaa agccttccgt
     5401 tgctgaaagt aaagaaaaca agattgtatt ttcgattgga ggggcgtagc tatgaaattc
     5461 aagaaatatt tactacaaaa ccttagaata aaacgatggg tcgtaagagg tctagttatt
     5521 ggaggagtat tagcagttac tttaggcgct ccttttggaa tgaatgatcc aactcctgac
     5581 aatcaagata gcaaacaaga aacagttata aataagaacg ttgcatatgc ggaaagtaaa
     5641 ccggcagaac aaggaatgtg gacactatat gctcgtatcc taatgggaga taaagaagtt
     5701 aatgatagtg ccgctaaaac tggacaaatg aatttgttag gacaaggtgg tatcagtgcc
     5761 gatttccctt attctgacat ttctaaacaa ggtaattcga ttgcaggggg taatgcaggt
     5821 actcaactag catctacttt agcaacttat agttactacg gatatattga aacagtctca
     5881 ggaaatgcta ttgccagtca agcatcatcc attctaggaa gtattggtag gacgattggt
     5941 agttttatcg gatcaattag tttaatcctt tctaatattg ctgaatccat aaatgaactc
     6001 cttggaaaat ttttcatgag tatgaatata ttttctctta tggggtgggg agaaggtgga
     6061 tcggatgtat ctaatccggt atctaaagca ttaactagct tgcttaacaa tttgggatta
     6121 aataatgaat tatttaaagc gatagcaagc ttatctatgc tctttttcac agcaatacta
     6181 ggttatcaag gaatgaaatt attgtcgaaa gatggtgtta actcgaaggg attatcacca
     6241 gtacttgaaa aatggattgt tcgtattttt gtattgttta tattcttacc agtggcagga
     6301 tcgctattgg gagtttatat caacggcatc cagtcagcaa aaaaagatgg agatatacca
     6361 acaaatgttg tttctgacta catcattaac acaagacttt gggcttctac tcaaaatctt
     6421 gctccatcat ctgatactgg tggtgatgta cctgacggaa agcctacatc cgaaatcaag
     6481 ttcgttgata cagattatga accatctaaa aaatctgcaa tgataagagc gatcaatact
     6541 agcagttatg ccagacaaaa taatgatcta gattctaatt cagtcggtat ggctctatta
     6601 aaagaatggt catcgaatga tactttcaat attaatactt atgccggtga tgttaatgtc
     6661 gccgaaaatg atgcttatat gggaagagcg cctgctgttt ggacaggcta taatttaggg
     6721 gatggaaaag aaggaagtgc aattaagaaa ccaaccaatt taaatggata tatttggtca
     6781 gcaaatcaaa atgtgacaga cgacaatcgc aagcctgatg gaaaaggaac agcgcataac
     6841 tttgatgcta ctcaaccttg gggcgttgct aacaatgcaa cttttagcac acagtcggta
     6901 gctttattgc tccaatcggc acttgataca tcgggagcta agttttatgc gtataatatc
     6961 gctccttcag gaattcaagg cagtgctaag aatatgtcaa cagtaaaaac agaatggcgt
     7021 acagcatcct tagttggtca tggagctatg ggtaagtttg gctcgtggct agcgatgaac
     7081 gctgaatcaa tcgttttagc agttctttac ttagcatgtg cttgggcgat gttcaatgtg
     7141 aatatcgtga aagcatttaa acgtgaattt attcggattt gggaagtagt tctaacagga
     7201 tcacttgcat caggagcagg tgtgatggca atttcattag gtgttgttac aacatctctg
     7261 atcggactac aattacctat ctttctgatt acttttattc gtgcatttgg tgatactgct
     7321 gtgggattca ttcctaatac attagttggc gatggcgtag caaatattgt tcgatctgct
     7381 atgattttag gtgtaggata tcttttagtc tttaaaaaag gcgaaaaagt aaataatttg
     7441 attaatatgc ttattggctt accacttgaa gtagcgtttg ggtttgatga acgtgctaag
     7501 ttattatttg gtaatagaaa tgatgctgtg cgagttgtgg acggcgtttc taaagcaatc
     7561 aagaaaagcc aaggagctaa aaatccctat tatgatggtg gcaacagctc agtaaaagat
     7621 atggttgcag gaggtcttgg agcagttgga gcgcaaaaaa tggctgaacg tttgaacaat
     7681 atgggagata gcaatgctga tgctaagtcc attgctgata aagtaattcc tgaaacacca
     7741 gaaggaatgt cattcaacgg tggcggagtt ggttccacaa tcaataatac ctctttcaaa
     7801 gatttagttc gtcaagatca actacgtaat aactcccaac aatatattga tcatttagga
     7861 cgtatggttg atgaaaacgg aaactttgtc gatgaaaatg gtgaaatatt aaaagatagt
     7921 aaatacggta aagatgctaa aggcaacttt attgacgaaa aagggcgttt ggtcgatgaa
     7981 gatggaaacc ttatagatac taatggtaaa ccattggaag atagtgaaga tggtaatatt
     8041 ggtatggata gcgaaggacg tttaattgat gaaaaaggtc gcttagtcga tgaagatgga
     8101 aatcttgttg acgaaaatgg acgcaggttg ctaaatggtg attttggtca tgatagtaac
     8161 ggacgtttag ttgacgaaaa aggtcgttta gttgatgaag atggaaatct tgttgatgag
     8221 tttggtaata aacttggtaa acacagcgat ggatataatg tgaatggaga tttagtcgat
     8281 gaggatggga atatcattga gacgagagat gcgcttgtgg cagatcgaaa tagagaactc
     8341 gaacaacaac ggcatacgcc aattgctgtt gcaattcctg agaatgaaga agacgagatt
     8401 actttagatc aaactcatca agaactgagg aatgaaatga gtggtgagga cgtgtcagac
     8461 agagtgcatc gggaattagg agatggagca tctggtgaaa gtcagtcaag agagcttaac
     8521 agcaactttg ataatagtgc tattcggaat gacacagtac ttaaagacag agaatcgcta
     8581 acaaaagaaa cggataaaga acaatcagat gtgaagaaaa agttcattcc tactcctaat
     8641 gttaatcaaa cagataaata tgaaacaaat attggatcag gaatgtcgaa agacaatcaa
     8701 tcaattagag aacctgaact gacaaataaa aataatagaa caaataattc taaatctact
     8761 tcagataatt cagggaaatc taatcaatca accaagaaaa catcaacagc taaatcagcc
     8821 gttgaatatc tatcaaaaac tggtaaaaat gtttacacct cagcagataa agctttagca
     8881 aaagctccaa aaggtagtct agcagggata acctatgaag ctaaaaacga agctattaaa
     8941 ataggtaaag gaacggctaa ggttgccaag gctactgcta caggagcatc caaaactggt
     9001 aaagtagtga agaaatctac catgtcagtc aaggactata ttgtaaaata cggtaatcaa
     9061 aaatcaactg aaaataagtc tgaagacgag taaaaagaag gagcattgat ttgctccttc
     9121 tttttacttt gatttctaac tgaagatgtc aaggaaaatt tctatttatg caaaaaaatt
     9181 attttaaatt gtctataaat gaattaaata tttactgttc aaagttgtat aattaaaaag
     9241 ggcttatgct ggtaaagaac agaattatta tctaaacaaa aacttgataa accagcattt
     9301 ttagccaata aaaaaagtga aataatttaa tggaaaatct cttttaatca tcg
//
LOCUS       DBKOIJ010000112         9235 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000112 DBKOIJ010000000
VERSION     DBKOIJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.114"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            6..653
                     /locus_tag="ACV5NJ_002077"
     CDS             6..653
                     /locus_tag="ACV5NJ_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HHY6486131.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            737..1681
                     /locus_tag="ACV5NJ_002078"
     CDS             737..1681
                     /locus_tag="ACV5NJ_002078"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HHY6486132.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1816..3267
                     /locus_tag="ACV5NJ_002079"
     CDS             1816..3267
                     /locus_tag="ACV5NJ_002079"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HHY6486133.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3288..4124
                     /locus_tag="ACV5NJ_002080"
     CDS             3288..4124
                     /locus_tag="ACV5NJ_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HHY6486134.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4124..4783
                     /locus_tag="ACV5NJ_002081"
     CDS             4124..4783
                     /locus_tag="ACV5NJ_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HHY6486135.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4909..5598
                     /locus_tag="ACV5NJ_002082"
     CDS             4909..5598
                     /locus_tag="ACV5NJ_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486136.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5604..6560
                     /locus_tag="ACV5NJ_002083"
     CDS             5604..6560
                     /locus_tag="ACV5NJ_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HHY6486137.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6604..7116
                     /locus_tag="ACV5NJ_002084"
     CDS             6604..7116
                     /locus_tag="ACV5NJ_002084"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6486138.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7372..7920)
                     /locus_tag="ACV5NJ_002085"
     CDS             complement(7372..7920)
                     /locus_tag="ACV5NJ_002085"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HHY6486139.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8009..9190)
                     /locus_tag="ACV5NJ_002086"
     CDS             complement(8009..9190)
                     /locus_tag="ACV5NJ_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HHY6486140.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         3055 a         1698 c         1864 g         2618 t
ORIGIN      
        1 aatcaatgaa aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt
       61 gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct
      121 cttcacaggt atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa
      181 gttctagtga agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat
      241 tgttccaaga taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg
      301 gatcttactt ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg
      361 atgctcaaaa tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa
      421 atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag
      481 cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata
      541 aagatttagg tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag
      601 tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc
      661 ttcggaaaac aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt
      721 tgaaggagcg atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc
      781 tttgatgaat gcatctggtg tccactgcat gactacccag gaattagatg aactggctca
      841 ttcagaagca ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc
      901 cgaaccacgc tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt
      961 aggtttttct tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa
     1021 tcaaccgtta tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga
     1081 gaaaatagaa aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt
     1141 gcctggaaaa ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt
     1201 attcagcatc ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca
     1261 ttttgatcag atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa
     1321 cagtgtgggc aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa
     1381 agaagggttc ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg
     1441 cgctttttat acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac
     1501 aggacaagat gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga
     1561 gcttcataaa gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat
     1621 gtcagaaaaa ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata
     1681 gcaaagagac gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt
     1741 cattcctgtg caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc
     1801 aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa
     1861 tttagttttc aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa
     1921 ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc
     1981 cagtcagaaa aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa
     2041 aaaaagggat ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat
     2101 acatctaatt atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat
     2161 gcgccagagt ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac
     2221 ccttggaacc ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca
     2281 tcaggaatcg ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct
     2341 tcgttttgcg gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat
     2401 gcttggagag gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac
     2461 acggaggctt tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg
     2521 ccagttgagt ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat
     2581 tcaccgatcc actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat
     2641 ttccttggaa aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa
     2701 ctgatcacgt cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt
     2761 gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg
     2821 ttccaatatg gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat
     2881 gaagcagcaa ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg
     2941 actgcattta ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag
     3001 atggaacaca tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt
     3061 gaggagagct tgaagatcac accttataca cagttggcta atctgactgg acaaccagca
     3121 atcagcttgc ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct
     3181 gccagaggaa gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag
     3241 ttccatttac caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa
     3301 gattgtttgc tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg
     3361 ctaatcagga agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga
     3421 cttctggcac actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg
     3481 atgtagattt ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat
     3541 acaacgtgga tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg
     3601 attttggtat cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt
     3661 catttgacag tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc
     3721 ttaaagggaa aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac
     3781 acggagctaa attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag
     3841 caaatggtcg aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg
     3901 ctttaccaga tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg
     3961 gttttttatt gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag
     4021 agatgaaaga agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt
     4081 cagtcaaacc gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcccctaat
     4141 tgatttttcc ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt
     4201 agggattggc ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt
     4261 aggtcttttg gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag
     4321 aggcattccc gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag
     4381 tgccttaacg gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat
     4441 ctatcgagga tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg
     4501 tttttctttc tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt
     4561 accggcactc agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat
     4621 cacagtgcca gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt
     4681 ttctctctat attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt
     4741 gcaacagtat ttagaaaaac gttctatcca tcagtcaatc tgaaaaatag aaatggtgct
     4801 agcgtggctg gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa
     4861 ccatttcatg taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg
     4921 ctaaccatcg aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt
     4981 gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca
     5041 aaatttgatg gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt
     5101 cgctctacag ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa
     5161 ctatatgaag aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg
     5221 tacacggttg aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca
     5281 aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc
     5341 agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa
     5401 ttgtacaaag aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat
     5461 tatgaacggt ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt
     5521 atcgcgcttc agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct
     5581 acagagccat tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc
     5641 tattagcatt aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga
     5701 tcgaaccagt acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc
     5761 accatccaat cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc
     5821 atgtgcacag ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc
     5881 cagaaaatct cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc
     5941 actacccaaa agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga
     6001 taccagatac tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact
     6061 cttttcaaac gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg
     6121 atgactatct ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag
     6181 gtagccgtta tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata
     6241 ttgatgctta cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta
     6301 aagaccaagc attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc
     6361 accatcatca actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac
     6421 aaaaattccc tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta
     6481 ttagtcacct gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac
     6541 tatatgaagt gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac
     6601 tggatgtata ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat
     6661 gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca
     6721 acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa
     6781 gatcaaattg ctggaacagc tgcattagtc tcaggcgtgg atcccgttta cacaaaaata
     6841 gatggtagtt ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca
     6901 gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca
     6961 gccagcaaaa atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa
     7021 aagaccatct attctgctgg attcacttat caagggatga ttgaatttcc ttttgaacac
     7081 ggcgaaagaa aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat
     7141 aaagacatta acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag
     7201 tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat
     7261 tttagcgtat tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca
     7321 tagcttttgt cacgatctct cggcttatcc tacgagtatt attcgataca tttatatcaa
     7381 taatcctttt tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa
     7441 aagtgagggg atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag
     7501 tctggctgaa atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc
     7561 tttagagatc gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca
     7621 aaaaccattt gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc
     7681 tgctttactg gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc
     7741 cttgatcatc tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc
     7801 cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc
     7861 ttctgcgagt gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat
     7921 tgtcatttct cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct
     7981 tttaaaagta actttgcttt tttgtatttt ataccagcaa gccagtcaca atagcactta
     8041 aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc
     8101 ctttttcttc atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg
     8161 caaatgaaac gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac
     8221 catttgctaa ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac
     8281 taccggcatc cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga
     8341 tgtaacctag gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca
     8401 tggcaatcaa agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac
     8461 catcaagaat gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga
     8521 tcagctcatc ttctttttct tctaatacat agggatttac aattgaagcc agaatataac
     8581 cgccaaataa gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat
     8641 aagatccgac gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac
     8701 gtttctcaga taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta
     8761 aaatagcaga agctactcca ttataagatt ccaatttgcc catcccgttg attttactta
     8821 atcctagacc aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga
     8881 taatagcaga aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt
     8941 tatttgctac accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat
     9001 caaacatttt tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga
     9061 aaccaagaac aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt
     9121 tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata
     9181 aataactcaa ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgc
//
LOCUS       DBKOIJ010000113         8902 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000113 DBKOIJ010000000
VERSION     DBKOIJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.115"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(258..1214)
                     /locus_tag="ACV5NJ_002087"
     CDS             complement(258..1214)
                     /locus_tag="ACV5NJ_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HHY6486141.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1318..1920
                     /locus_tag="ACV5NJ_002088"
     CDS             1318..1920
                     /locus_tag="ACV5NJ_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HHY6486142.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(2008..3507)
                     /locus_tag="ACV5NJ_002089"
     CDS             complement(2008..3507)
                     /locus_tag="ACV5NJ_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HHY6486143.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3523..4047)
                     /locus_tag="ACV5NJ_002090"
     CDS             complement(3523..4047)
                     /locus_tag="ACV5NJ_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HHY6486144.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4345..5094
                     /locus_tag="ACV5NJ_002091"
     CDS             4345..5094
                     /locus_tag="ACV5NJ_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HHY6486145.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5085..5870
                     /locus_tag="ACV5NJ_002092"
     CDS             5085..5870
                     /locus_tag="ACV5NJ_002092"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HHY6486146.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5839..6555
                     /locus_tag="ACV5NJ_002093"
     CDS             5839..6555
                     /locus_tag="ACV5NJ_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HHY6486147.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6657..7676)
                     /locus_tag="ACV5NJ_002094"
     CDS             complement(6657..7676)
                     /locus_tag="ACV5NJ_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HHY6486148.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7695..8696)
                     /locus_tag="ACV5NJ_002095"
     CDS             complement(7695..8696)
                     /locus_tag="ACV5NJ_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HHY6486149.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         2677 a         1799 c         1593 g         2833 t
ORIGIN      
        1 gccaaacaaa aatagctcct cctatttttc gtttggctgg acttattacc gcagaggaaa
       61 gcttttgaac accgtttaag aaagaggttg tgagcctacc gttcagctcc gagtcacaag
      121 gagcaatttc aaaaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag
      181 ttgacaggag aacaccgttt aaagaagagg ttgtgaaatc agctttttga cctgaatatt
      241 agctgaattc acaacctcta tttttggatt ttttgttggg agaattctgc cttgatttca
      301 ataaaacttt taaaagactg caaaaactgg aacaattctg ctcgatcctc gaattcaggg
      361 cgactagtcg gcagaggcat ttgccgatac aatacataaa cttcttcaat acgtgttaaa
      421 atttgtctgc catcatttga ctctgaaaat gtttctgcag tataaatcaa taaaccatag
      481 atatgttttc cataataagc tggttgctgg attctctcta gattttccag catgtcttta
      541 atcacattgg cctgtgctct tctcatagag aagtaagttt cgtagtatag cggctgtctg
      601 agccagtagt tttcttgatg ttctctggca tcttcttggc tttcgcgaat gaatgtcagt
      661 aaatgttcgc atttgatctg cacatcttct ttattttctg ataaaagaaa ttctgccatt
      721 tgtctcaaaa tatttcgaaa ttctttttcg atctgtattt ggttttcctt cagctgcttc
      781 gtacgatctg gcatgtaaac atttgcaagc aaagcaaata ctaatccaat cgccattaat
      841 cccgcttcat tgccgatcag ctgccaagac atgttttcct ccactaaata atgagtgacc
      901 aaaaccgaat ttactactat cccttcggtt aatttgaagc gagaggcaag cgggatgaac
      961 aggaataaaa aaagactgaa gccaaaaata gtatacccaa cagcaaaaaa actgaacagt
     1021 gctgttgccg tagctaatat aaaggctgcg attcgattca tgcccgtacg caatgtagag
     1081 cgcttggtat ttcctacact taataatgca atgatccctg cagctggcca atacaacagc
     1141 gataagctac ccgctgtgac catcgaaaag aagacactga tcactgtttt gactgttcgc
     1201 attcctatac gcatttactt cccccttttt tattttaatt atacttgaat aatgaaaaac
     1261 tagctatcag acaaaagttt aggtatgata gtaaaaagac caaaaaggag aatctctatg
     1321 caatttggcc gttttcgact aggtatgcgt acgatcaaaa gtgccttagc tgttttttta
     1381 tgtatcttgt tttttcattt gactcaacga ggaatcccga tgattgctgc attatctgct
     1441 gttttttctt tgcgtcagga cttgactaca accgtttctt ttggaaagtc taggatcatc
     1501 ggcaatacat taggtggtac attagctatc atttattttt ttgtcaaaag ctattttcac
     1561 aatgattttt tggtagaatt gatcttgctt cctattctag ttattattgt gatcgtcgtg
     1621 tcagatggta ttaacaataa ttccggtatc atttccgcaa tagctacgtt gttgctgatt
     1681 gcgttgagca tccctcaagg cgaatccgct ttgtatgcta ttcaacgtgt actagatact
     1741 tttataggga cattcattgc aatcggtatc aatttctttc ttcgtcctcc tgaaacagaa
     1801 aagaaggaag aactatcaga agatttggtc gaactccaaa agaaagaacg attgctaaga
     1861 acaaatttac aagagataga agctaagatc caaaaacaaa agaaaaatag cgaggactaa
     1921 ttttcttttg caataaaata aaaaaatgga gagaagaact ttaaaaagtc ttcctccatt
     1981 tttgattgaa cggtgaagtg tactacttta ttcttccttc atcgagtttt gttctgcctc
     2041 ttttggcgct tcatacatat ttacttggct agtagtccca cgtgtcgaca aattctctac
     2101 gatttccttc aagtcaagtc ctgttgtcgc ttttagtgtt tcttgagtag aagaaagcaa
     2161 atttgtggca taatttgtca ctcggttcgc tccactggtt tctcctgaac tgcttcctgt
     2221 atcgacaaca gagatttttt cgatatttcc tagcggctgt gctgcttcgc gcatcaattg
     2281 cggtagcata tcgataacca tactcaagac agctgcttcg ccatattcct tgaaggcatt
     2341 ggcgatcttt tgttttgctt cggcttctgc ttttcctttt gctagtgctg cttcggcttc
     2401 tgcttgtcca gctaaacgtg tcttattggc ttccgcttct gccagtgctt ccacacggaa
     2461 tcgttctgct tcggcttcag tcacttcttt gactttttga gcttgtgctt cttgttcttt
     2521 agcgtaccga tcggcatctg cttttttctt cacttccgaa tcatattgct tttcacgacg
     2581 tgtgatttct ttttcttcca gttcgatttg tttttgacgt tctacgattt taatctgcat
     2641 ttcctgttcg attacttgtt gttgtgctcg ggcactttct aaattgtagg cttggtctgc
     2701 ttttgcttta gcaacatctt gttcttgttt gtaggttgct aatttcagtt ctttttcttt
     2761 taatgattct gcgatctctg tttgtcgctg taattcagct gcttgtgatt ctttttctgc
     2821 ttgagctttt ttgatacgcg tttcttttag agcttccgct tctgctatat ctgcatctct
     2881 tttgacttgg gcgattcttg gtttccctaa agagtcaaga taaccgtttt tgtctctgac
     2941 ctctttgata gtgaaagaaa cgatgatcaa ccccattttt gcaaggtcta cagaagcgac
     3001 ttcttgtacg ctttgactga acttatcgcg attttgatag atttcttcta cagtcataga
     3061 accaagaatc gagcgcaagt gtccttctaa tacttctctt gcttcatttt ccagttcttc
     3121 tcttgtcttg cctaaaaact gttctgctgc tgtagcaatc tcttctacag atgaaccgat
     3181 tttgatgatc gaagtaccat cacacatcac cggtacgcct tgttccgtat acacttcagg
     3241 ggtggagacg tccaatttac tcgataatag actgatccga ttcgaacgtt gaaaaactgg
     3301 caagacgaat gcgccacctc cacgcacaat tttgattttg ttattgcttt catcaatgtg
     3361 cacatttttg cttcctaaat aacttccact gatgatcaac gcttcatccg gttttgctgt
     3421 ctggtacttc gctacaaaga cgatcagcaa catcagtaaa atcaaagctg cgattgcgat
     3481 cgcgataaaa acaactggta tttccattta acacacgctc ctttaagctt ttatcatgct
     3541 ttcataaggc atgacataac agattctttc cttgatctca atgatcaata ctttcgttcc
     3601 ccgctcagct gtctgttctt gggggctgta aaaacaagct ggacgactaa tggaaccggt
     3661 aacacttttc aactgaattt ctcccatacc tcgtacaggg atcggtgtga tcacaacagc
     3721 tacttgtcct tccagagttt tttcagaaga cgacatcgtt gattcggcat gtttcatcgg
     3781 catcaatata tagaaattaa gcaaaaatac caacactatc gatatgcctc cactagcaat
     3841 aaataaaaat aaagatgatt gatctgttaa acgctcacct agaaaaccaa ataagcttgt
     3901 gaaagttaac caagggataa taagcatcgg atcaagcggg ccatcaaaat cgaatacatc
     3961 tccaaaaata atcaatagga ctgccaatcc tgcacaaatc agcaatgtat accaataaat
     4021 cgtctcaagt gcgattcccc ctatcatata cacgcctcct tccatttcta cgtctatcat
     4081 accaatgttt gaaaattctg tatagcttct tttaccgact cagtggtgtt ttttatagga
     4141 aggttgtgtt tatcgttgac aatttgttaa atttttgttt tcatgttaaa attttcacaa
     4201 taaattcttt cgaggtagcc tatgtttcct ttttttaaga aaaaaccatt aaagctccag
     4261 aacacgatca tccttgtcgt ttgtttctcc acattcgttt ctttgattgt ttctgccata
     4321 ttgatcagga attttattct ttcgatgaac cgatctagtg tgtcagataa aatctctagc
     4381 attgctaaaa tcgttgccaa tgataaactc atccaaaaaa agctgcagac tggtgacccg
     4441 acacatgcga ttcaaggaat aacaaatgaa atcatgattg catcgaattt agattttatc
     4501 gttgtcatgg accctgaagg cattcgatat tctcacgcag acccaagtta catcggtctt
     4561 cctttctcaa gtcatgaaga tgtgaaaaaa gtgattggag gacaagaatt tttctctgaa
     4621 aaagaaggaa tgttagggtt cggcttccgc tatttcgtcc ctatttatta tgatcaagag
     4681 attattggtg cagtttgtgt tggcttaaca aacaacacga ttgacaatca agtcaaagat
     4741 ggacaaaaaa tgattgtttt ttcattgatt attggactta gcgtaggcat cataggcgcc
     4801 gtttttcttg cccaaaaaat gaaaaaagta ctgcttggac ttgaaccaca agaaatagca
     4861 gaacagttaa gtgaaaaaac gattatcgaa aatgaagtag atgaaggaat cttagccatc
     4921 tcttctgata aaactatcct cttgatcaat acacctgcaa aaaacatttt ctaccaaaca
     4981 gagcagctat tggatttcga agtaggaaaa aaatcagtga taatctatat gacgttttgt
     5041 ttaaagagac gattgagcat caaacgaccg ttcggactca atccatgtat ttaaatggga
     5101 tagaaactat ttctagcgtt acacctcttt ttagcaatga ccgtttttta ggagcagtcg
     5161 caactttacg aaatcgatca gaagtaatga aattaatgga ggagcttggg gggacgaaac
     5221 aatatataaa ctccatgaga gctcaaaccc atgaattcat gaataaactt catgtcattt
     5281 ctggtttgat tgatttgaag aagtatgaag aagtcaccca ttttatccag cagctaaatc
     5341 aaaattataa aaaagagatt ggccaagtct caagtctgat caaagtacct gcaattgccg
     5401 gttttttatt aggcaaaata aacgaagcca aagaacaatc tgttgaaatg ctgttagaaa
     5461 gcgattcttt tatccctaaa atggagatgg atgactccat ccattcactt ttgcaagtgt
     5521 tagggaactt acttgataat gcaaaagaag ccgtgctaca aaaacatgaa agaaacggac
     5581 aggtccggat tcatttaaac tatgacacag aagctgaagt attcatcgtg accgtctgcg
     5641 atgacggtgt cgggatatca gaagaaatct cagaaaaaat atttacgcta ggtttttcga
     5701 cgaagggaat caatcgtggg tttggattga atctcatcca ttcaatcgtc aaacgccacg
     5761 gtggattttt agaattttcc aaacaaacaa atggcggtac aatcgtctat ctagaattac
     5821 caaaaaagga agatcaaaat gatgacaaat gtcctgataa tcgaagatga tccaatggtt
     5881 gcagaattga atcgtacttt tgtggaaagc atccctaata tgaacgtaat cgaaagtttc
     5941 agaaatgtag aagaaggaaa aaaatatttc agacaaaaaa acgcagatct gctgctgctt
     6001 gatgtataca tgcctggcga aagtggtatc cattttttat cttggcttag acagcaagga
     6061 aatgatatcc cagtgatttt gatcactgct gcagatgata tcgaaaccat aaagaatgtg
     6121 atccgctacg gtgccttaga ttatttgatc aaaccgttca ctttgcaacg ttttcagctg
     6181 gcgatcgaaa agtatcttgc tttctctcag acaatccaaa cgactgaaaa agccgatcag
     6241 aaattgatcg atcaagtatt caaccacacc actcccattc acgaaatacc atcattcatc
     6301 gatttgccta aaggtctgtc aaaactgact ttaaaaaaaa taagtgcatt tattctaaaa
     6361 gaagatacta ttttttcaac aaaagaactg gctcaaaaaa gctcactgtc acgtatatcc
     6421 gtcaagaaat atttgaattt tttagtagaa caagatatat tgaacgaaag aatcaaatat
     6481 ttggaaatcg gccggccgct aacctgttat gaattgacaa agaatgggcg gaaaaagctc
     6541 acagattact gttgagtcat cgaattatcc catacaaaat agtccaagta tacagaagta
     6601 acaactgttt acttggacta tttttaaaca ttttattgac gaatatccat gttttcttat
     6661 cttaattgac gggctttagg aagagatacc tcatttttcg tttttccagt ttctattatt
     6721 tcacgaaagt tctcgaaact tgtcgaaatc atatttgata atgcctcatc tgtatttgaa
     6781 cctacatgcg gggttacaag aactctaggg tacatttcta ctaattgctg gatagcaggg
     6841 tctggaatcg tctcccatgg cttgaaggat cggaaaaaaa cctctgtttc atttgcaaaa
     6901 acatcggttg caaaaccagc taaatgattg cttttcaaag ctcttaaaat agcttcatta
     6961 tcttgaagct ctccacgggc tgtattaatc aagatgctac cttttttcat ttgagctaaa
     7021 aatccttcat tgatcatttg atcgttttca cctgggatat gaggtacatg aatactgaca
     7081 atatcgcttt tggctaataa ctcttcaagc gagtggaacg tcaatacttt tttagcagct
     7141 tctgtctgat gaatatcgta gccgatcact ttggctccta gtcccttaaa caaaacagct
     7201 tccgtcaacc ctatcctgcc tgttcctatg atccctactg tgcaatttct gatttcacga
     7261 ctaaacattt gctcatccac tcgaaaatct ttgaaagatg ttcggatcgt cgtatacgca
     7321 gtatttctca aaagcatcat ggcaaaagtc aaagataact ctgctatcgc atttggcgaa
     7381 tacgatggta cacgggctac tgtcatacca aaatcagctg ccgcttgtag atcgatatga
     7441 ttgactccca cagtccgtgt aaaaacataa cgaatcccat attcctgcat ttttgcaaga
     7501 ttggtctcat ctgctataca atttcccctc aacaatacag catcatggtc ctttgctgac
     7561 acgatattgt catgtgtcaa cagttcttca taaagactga gatcataatg gtaaatattc
     7621 aatttttcaa aaaaatctat ctcgttcgga cgaacaccgt aacatgcgat tctcatactt
     7681 tcttcctcca tttcttatga aaataaacct atgccatgaa tcatttgtaa tacaacgatc
     7741 catacaggtg tcagtaccac agcaaaaata gttgacagta aggatgcatt ggaggctaaa
     7801 agtgcctccc tatcaaaact gatagcatag gctgctgcca ctgttgcagc tggtgtagcc
     7861 atcatgataa cgattgttgc cagtgcttca aaattgactg gtagaatatt cgttgccgtc
     7921 aaaactgcta gaatcacgat attcaatgca ggtacaaata ccactttgac tagactgtaa
     7981 taccatgaag ttttttcaga tgcagctttt ttgaagctta cttcacctaa tgttgcgccg
     8041 attgctagcc acgccaatgg tgaacttaaa ccagctaaat aatccatcga ctgaaatagc
     8101 cacggtgcag tctggtcaat tcgcaaaaac gctacttggc ttatctctcc ttctgcacct
     8161 gtaactgaca cttgtggcag atacccttgg aacagccaaa tgaaaaggcc ggcaaaagta
     8221 gcgataatga ttggattgac gaacatcgct ttgatatttt tagcttccat cttcaagcca
     8281 ctcattttta tgtatccata agaatacaaa aagattcggt agccaatatt gaaaattgag
     8341 gaatacatca cgccgacagg accataaatc gcactgacga taggcgttcc gaaaaaggtc
     8401 gtagaaccaa agatcgtcag gacacgcaac gttgtttgct tatccccttt atagcttaag
     8461 aaaaaaggtt ttgaaactag aataagaata atataaataa ctaatcccca aatcaagaca
     8521 ttgattcctt gcatcagtaa ttctgtattg atatcttgca tgaaagctcg aaatgccagt
     8581 gctggcaaag ccactgttaa gacgactttc gatagaattt ttccaacaga ttcagaaaaa
     8641 atcccctttt ttctacaaac aaaccctaat aatataataa aaactgttga agtgattgca
     8701 ctgacaatcg acatatctgt caaagtcgat cgaaaaatgt caaataagtt catctttctc
     8761 ctcctaatta tgttcattta ttcacaataa aaaaagcttt tattttgcta atacgtatta
     8821 acatttttaa ttttatcttt acattgttac ttattgaaca ataggaaatt aaagaaactt
     8881 ttgaaactta tgtaaagtga tt
//
LOCUS       DBKOIJ010000114         8701 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000114 DBKOIJ010000000
VERSION     DBKOIJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.116"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            33..1094
                     /locus_tag="ACV5NJ_002096"
     CDS             33..1094
                     /locus_tag="ACV5NJ_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HHY6486150.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="ACV5NJ_002097"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="ACV5NJ_002097"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HHY6486151.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="ACV5NJ_002098"
     CDS             2007..3989
                     /locus_tag="ACV5NJ_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HHY6486152.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACV5NJ_002099"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACV5NJ_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HHY6486153.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4711..6135
                     /locus_tag="ACV5NJ_002100"
     CDS             4711..6135
                     /locus_tag="ACV5NJ_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HHY6486154.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6316..7089)
                     /locus_tag="ACV5NJ_002101"
     CDS             complement(6316..7089)
                     /locus_tag="ACV5NJ_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHY6486155.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7086..7991)
                     /locus_tag="ACV5NJ_002102"
     CDS             complement(7086..7991)
                     /locus_tag="ACV5NJ_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486156.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8145..8465)
                     /locus_tag="ACV5NJ_002103"
     CDS             complement(8145..8465)
                     /locus_tag="ACV5NJ_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HHY6486157.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2723 a         1571 c         1864 g         2543 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata
     4621 tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat
     4681 aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt
     4741 ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt
     4801 attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta
     4861 ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat
     4921 ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc
     4981 agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg
     5041 atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc
     5101 gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat
     5161 gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt
     5221 gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt
     5281 atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg
     5341 gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa
     5401 aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac
     5461 gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag
     5521 gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg
     5581 atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg
     5641 attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc
     5701 aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt
     5761 ttactttcat tattattcac tgacagtgct tataactttg cttacacact tggtacagca
     5821 gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg
     5881 aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg
     5941 ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat
     6001 ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag
     6061 attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg
     6121 atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat
     6181 tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa
     6241 taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc
     6301 ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa
     6361 caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg
     6421 cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca
     6481 tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc
     6541 aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat
     6601 catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa
     6661 caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa
     6721 aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata
     6781 aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag
     6841 gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat
     6901 catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc
     6961 aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag
     7021 aacagataaa acagcagttg actttttctt gatcaattta aataattctt gttttacaag
     7081 caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc
     7141 aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg
     7201 atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc
     7261 aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata
     7321 acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt
     7381 ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg
     7441 gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt
     7501 gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc
     7561 agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt
     7621 ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg
     7681 ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata gatacccggg
     7741 tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt
     7801 tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc
     7861 gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta
     7921 aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa
     7981 agacttttca aaaatattca ctccttttct atagatagta caagcataca cctgatgagt
     8041 ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta
     8101 aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta
     8161 atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc
     8221 agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc
     8281 cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa
     8341 ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc
     8401 catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat
     8461 gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct
     8521 tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt
     8581 tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt
     8641 accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata
     8701 g
//
LOCUS       DBKOIJ010000115         8569 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000115 DBKOIJ010000000
VERSION     DBKOIJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.117"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(167..2548)
                     /locus_tag="ACV5NJ_002104"
     CDS             complement(167..2548)
                     /locus_tag="ACV5NJ_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HHY6486158.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2538..3185)
                     /gene="recU"
                     /locus_tag="ACV5NJ_002105"
     CDS             complement(2538..3185)
                     /gene="recU"
                     /locus_tag="ACV5NJ_002105"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HHY6486159.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3249..3788
                     /locus_tag="ACV5NJ_002106"
     CDS             3249..3788
                     /locus_tag="ACV5NJ_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HHY6486160.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3878..4297
                     /gene="gpsB"
                     /locus_tag="ACV5NJ_002107"
     CDS             3878..4297
                     /gene="gpsB"
                     /locus_tag="ACV5NJ_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HHY6486161.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4319..4686
                     /gene="rnpB"
                     /locus_tag="ACV5NJ_002108"
     ncRNA           4319..4686
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACV5NJ_002108"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4895..6061
                     /locus_tag="ACV5NJ_002109"
     CDS             4895..6061
                     /locus_tag="ACV5NJ_002109"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HHY6486162.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6107..7603
                     /locus_tag="ACV5NJ_002110"
     CDS             6107..7603
                     /locus_tag="ACV5NJ_002110"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HHY6486163.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7684..8367
                     /locus_tag="ACV5NJ_002111"
     CDS             7684..8367
                     /locus_tag="ACV5NJ_002111"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HHY6486164.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8376..>8569
                     /locus_tag="ACV5NJ_002112"
     CDS             8376..>8569
                     /locus_tag="ACV5NJ_002112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486165.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2681 a         1565 c         1823 g         2500 t
ORIGIN      
        1 aaatggattt tctaattttt aagctaaatg ttagatcaca catgtatatg ctggatagtc
       61 ttataaaaaa tcatgtaata cagcttggta catgattttt atttgtagct gttttttaca
      121 tagacacaaa aaagaaacca cgcttgtaga cgtggtttct tttttactat ctgtttccag
      181 aagctgaggt gctgctggat ggtgtatggt catttgcagc atccgctccg ctagttgctg
      241 gctcactgga agaagcagga ggagcagatg aagacggtgt cgagctttct tcggatgaag
      301 aagaaggaac cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg
      361 aggactcagc agttgattcg ctttgactag tagagctaga gctttgagtc gttgtctctg
      421 ttgttgagct tccaggagtc tggacatagc tggaagatgg aatagttgtt gatggtgtaa
      481 ttgcattcga tctagctgta tattgatctt tgtagtacag ttcaccgccc acacgaatta
      541 atccgcttgg catctcccag tctgtatttg tcacgttagc tgaaacatac tgcataagct
      601 cgcgataaac atcagaggct acgtgtgacg attctgaagt gacaggcgtc atttttttat
      661 tgtaacctgt ccacacggaa atcgaataat ttggcgtata gcccgcaaat aggatatctg
      721 gatacacccc agaagaaata ccaagttttg catactcgtc atctgtgtaa ttggatgttc
      781 cggtcttacc ggcttgatat aacccagcga tttgcgcatt cgtaccagtt ccttcagtga
      841 tcgtatcttt taaaatgtct gtgatcatat aagctgtttc tggagacatg gctgtttttc
      901 catctggctc ataagtttct tctgttccgt cttggaagac gattttatta acatattgag
      961 gtttgtaata agtccctcca ttggcgaaag ccgcataggc tgctgccatt tttaaggaag
     1021 aagctccgta ttttgtccca tcttgttctt ccgtattact agagatcgca ttcgattgat
     1081 ggatcgtact gtattcgatc ccaagatttt ttaagaaaga agccacttta tctgaaccga
     1141 cttcattgaa taatttgact gctggaacat tacgggataa gtaaagtgct tggcgtaaag
     1201 taatggtgcc catatattga ttatcccagt ttcctacagg agtcgaagtc ccttcatagt
     1261 tatacggtgc gtcagtaata gtttttccag tagaatattt caaatattca aaagctggtc
     1321 cgtaatctgt gacaggtttc atcgtagaac caaaatcacg ggaagtattc actgctaagt
     1381 tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc aatctgggct tttactttcc
     1441 cggtattggt gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg taagagacat
     1501 actgatccgt gttgacgata tcatatagtt tcttttgggc gtccagatcc aaattcgtat
     1561 aaatctctaa accgtctgtg tagacattct tatctgtttt ttcttgtact tcattgatga
     1621 cttctttgac ataattatcg acgatcttag tattgtcatc ggattgagtt agttcttgca
     1681 gaccatccgt taccggtaca ttaacagctt gatcatattc tgtttgagag attttttcgt
     1741 tttgaagcat cgtatacaaa acagtatctc gtcttttctt cgcctgatct ggataaacat
     1801 aaggatcata ggcggaaggg gcttgaggca tcccagcaag caatgctgtt tgcggtaaag
     1861 aaagttctga taatttctta ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc
     1921 cgtttgacat atacacttta tttacgtagt aggtcaagat ctcttgtttc gatttttttt
     1981 gttccaatct tacagccatc cacgcttctt gggccttgcg tttcagagtc tgatcctcgg
     2041 cactggtgga gaagaaggaa agtttgatca attgctgtgt caaagtactt ccgccttgca
     2101 gtccgccaga ggtaaagtta gagagggcag aaccgatgat ccgaataggg tcaaccccga
     2161 tgtgtttata aaaacggcga tcttctaccg acacgatcgc atcttctaat gttttcggta
     2221 attcattcgc agaaattttt tcacgctttt ctgcgcctaa atcttcgaat agttcgccat
     2281 cttgtgtata tagtttggaa gatacggtag cgtctaattt tttatccgtc aattcgggcg
     2341 catcttttgc gtaataccag aaaagtccta cacctgctaa aaagaggata cagaaaaatg
     2401 agagtattcc taaaaggatc ttgataaata atcctttttt atgtgttcca gaggactttc
     2461 cagaaccttt gccagaattc ttcttcacac tctttttagg agtaggctgt ttctgtcgtc
     2521 tagagcttct agtttgttca tttgccattc ttgctttctc cttcaataga taatgttgat
     2581 aaatagaagt caaccgcgtc caaataagga atccgcggag aaataccagt tttgatctct
     2641 attcctagtt cttcgatcaa agataaaggt agtgatttgc gtcctttttc cttttgggcg
     2701 tcccagtaag caatcaaatg aacactattc aaataaaaac agcgcttcaa agacgaaaac
     2761 cataacaaga caaagcagac cccattttgt tcgaggcatg ctttcatatg ttcgatttga
     2821 tgggcatgga agtttttaaa gggaaaagat gttttgttct tggtttcttt tgcttcaaag
     2881 tccaagtaaa agcctcgata gacgccatta tagtctgtag ttgaggcttg ggtaaagtag
     2941 gcttccttga tcacagcagc acttctctta ggatagtcta ctttcacgat ttggataggg
     3001 gtaggctttt tatggataac cgccaacccg cgggaaagat aatatttatt actttcattg
     3061 attgcttctt caaagcgcat cccccgattg ccgaattcag tgatctgatt tcttttttct
     3121 tgaatttttt cgacgggaga ggcattctta taataaggtt ggccatttgg atagcgcaat
     3181 accatcttta atcacactcc taggccacat tatagcaaaa aagcggatag aaagaaaagg
     3241 aatctgagat ggataagtca aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg
     3301 gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac
     3361 tccatcagtt agcagaagaa gggctggaat gggtacttgt cagtgggaat ttgggcgtcg
     3421 aactatgggc tgtgcaaata gtagcggaat tgaaagaaga atatccggaa ctatctcttg
     3481 gtattttgta tcctttttct gattttggta ataactggaa tgaagggaac caagaaaaaa
     3541 aacaattagc agaacaatta gcagattatg tcgaagctgt tagccatcaa ccctaccaat
     3601 cacctagtca gctaaagaat cacactcgtt ttatacttga gcatacagtg ggctgtctgc
     3661 tgatctatga tgaagaattc cctggaaaaa cgaagtattt ttttgaagat gcacagaatt
     3721 accaagcaga tcatccctac gaaattcggc tgatcagtat ggacgatctt caaaatttta
     3781 gtgaataatc gatattgtgt ttgatttatc aacttttttc ctatacaatg aaaaaggtag
     3841 aaatgttatt acgtaaatga aaactagagg tgtaaatatg gcgaatttgg tttatagtcc
     3901 taaagacatt ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt
     3961 tgatgaattc ttagacaata tcatcaaaga ttatgaaact tacagcaaag aactccttgc
     4021 tttacaagaa gaaaacgatc gattgagtgc aaaagtagct caattatcta aaactcaagg
     4081 agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag tgaccaattt
     4141 tgatatctta aaacgcttgt caaatcttga acgtgaagta tttggtaaaa agcttgatca
     4201 acaagcaagt gcagtcaaac cagcacaacc taatccaaat aactacacaa atgcagacac
     4261 aagcttagat gataatgaaa aaacacgtca attttaattt ttgattaatg aatttgcggt
     4321 tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa gtccatgctc gcacaggctg
     4381 tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta aggtactctt tggagtaacg
     4441 gcaggaaaac aagctaaggc ttcggctatg cttaagtatc cttgaaagtg ccacagtgac
     4501 gaagcggctg gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa
     4561 acaatggtag gggcgctttt tgtctggaag aagaacagga caaaaaggca gtataactgc
     4621 agatagatgg ttaccgcttt gttttttctc cctggaaaag caaagtacag aacatggctt
     4681 atagaaaacc gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt tataagaaac
     4741 aagaaaaaca tttgaaagct atttttcaat acttcagatg aatcgttctt attacaaaac
     4801 aaacttttgt atcaccggtt attcggaatt tgagtaaaag tgtggggtag gactgatgtc
     4861 ccacctcctt tttttataaa taaggagatt gtgtatggaa aaaaacagga agttcaacct
     4921 cgtcgcaact gcggcaagcg ggctagaagc actggttggt aaagaattaa gagatcttgg
     4981 aatcgaatgc caagttgaga acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc
     5041 tacagctaat ttatggttac gtacagcaga tcgagtaaaa atcgtggttg gggaattcga
     5101 tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg aagattttct
     5161 tccactagat gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac
     5221 acctgattgc caagcgatca caaaaaaagc aatcgttgaa cgtctccgta cctattatca
     5281 ccgtccagct tctgtaccac tgacggaaac tggcgcacat tttcagttgg aagtagcttt
     5341 gctgaaagac catgtgatgg taactctgga tacgacagga ccaagtttat tcaaacgtgg
     5401 ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag cacttattat
     5461 gttgacaaat tggcgaaaag accgtccatt ttatgatcct gtatgtggtt caggtacgct
     5521 ttgtattgaa gcagccttaa taggccataa tatcgcacca ggattcaatc gtgagtttgc
     5581 ttgcgaatct tgggattggt tcagtcaaga aatcatggaa aatgttcgat cagcagccga
     5641 agaaaaagca gattatgata ttgaattaga tattactggt tcagatatca atggacggat
     5701 gattgaaatt gccaaagcca atgcagaaga aatcggatta ggctcttcga ttacctttaa
     5761 gcaacaagca gtcaaagact tcaaaacgga taaagaatac ggggtcatcg tggctaatcc
     5821 gccttacgga gaacgtctag gagaagaaga aactgttcgg aaattatata aagaaatggg
     5881 cgatgtattc cgtccattaa aaacatggag taaatatatc ttgactagcg acttagcgtt
     5941 tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg
     6001 tacggatcta ttccagtatt ggggaactcg tccgcctaga aaaaaagaga aagacgaata
     6061 atcttactaa ttgattcatt caattggatg tttggaggaa tagggaatga aagaaaaaga
     6121 gtttttacaa gaagtaaaag aaatcaatct tctacaacaa gctttaggaa tcctcgattg
     6181 ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta
     6241 tctttacagc atttattttt caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta
     6301 tttttctgaa catccggaag aattgtcgga agttggaact gccgtttttg ccaaagtaaa
     6361 agaagagtat gatttggaac atcgcgtacc ggaagatctt atgagaaaat atacagctgc
     6421 cgtttcttca gcacacggac aatggcaaaa agctagagaa acacaacaat tttctgattt
     6481 tcaacacgca ttgaatcaaa atattgaatt aacaaaacag ttgatacctt actggagaaa
     6541 agatgaaaag acgaattatg atgttttgct gaatcaatac gaacccggga tgactgtcga
     6601 aatccttgat aaggtatttg atcagcttaa agaaggaatt ctttcgatcc gccgtacgct
     6661 ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca
     6721 acagaagaga tttgtagtga aagtaatcga acaacttggt tatgatttta gtcgtggtcg
     6781 tttagatgac actgtccacc catttatgac tggaattaat ccgaatgatg cacgaatcac
     6841 gacacgatgg aacgaaacag atttcaaaat ggctgttttt ggagtgatcc acgaggctgg
     6901 tcatggtatg tatgaacaag acatcgatga taaatatgcg tatacaccgg tttatgaagg
     6961 tacatcaatg ggtatccatg aatcacaatc gttgtttaac gagatcatta ttggaagtag
     7021 tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag gaacattcga
     7081 tgatattcct tttgacaaat tttatcgttc attgaaccat acaaaatcaa gtttggttcg
     7141 tatcgaggca gacagtttga cttaccctct ccatatcatc attcgttatg aaatcgaaaa
     7201 aatgattttc aatgaagggg tagatgttgc tgaactacct gaaatatgga atcaaaaata
     7261 tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac aagacgttca
     7321 ttggtcaggt gcaagctttg gttattttcc ctcttatgca ttagggtaca tgtatgcggc
     7381 acaattactt cattctttgc agcaagaaat agatgtggaa aatgttctag cgtcagacga
     7441 ttattctcca atcaaaaact ggatgtcaga gcatatccat caatatggtg catctagaaa
     7501 accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat acctgatcga
     7561 ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga
     7621 tagctgaaat tcatctaatc tatagatgtt ttcagctatt tacttaagaa agaagtggaa
     7681 cgaatggata aattacaagt gatcgctgat tatagtatgg accaattagc gcatgatcaa
     7741 acaggacatg ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa aattttggag
     7801 acggaaccgc aggcagaccg ctttgtgacg cttgcagcag cttacctaca tgatacgatt
     7861 gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt attccttcgc
     7921 tctttgccaa tagtagagga acagatctcg atgatttttt ccattatcga aaatatgtcc
     7981 ttttctaaaa atctctctga agcggccgaa ctatctctgg aaggtaagat cgttcaagac
     8041 gctgatcgta tcgaagcatt aggagcaatc ggcattttgc gtactgctta tttcggcggt
     8101 ggacatgggc acccgatttt tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa
     8161 aattaccgaa aaggcacgac agtgatcaat catttttatg aaaaactgtt cttgctgtct
     8221 gataaaatga atactgatta cggatatgaa gaagccaaac gaagagaaac cttcatgcgt
     8281 gaattcttag aagaattttt tctggaatgg tctattgacg atgaaaattt tactccagac
     8341 aattggatca aaaataacaa aaaatgatgg aggttgtgac agaagtggtc agctccaaga
     8401 tatttcggat cattttccgt aaaagttact tctggaacac tgtttaccaa gaggtcgtga
     8461 gcctgccgtt cagctccgag tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga
     8521 tgaatattgt aacttgtgat tcggaattgg caggagaaca ccgtttatt
//
LOCUS       DBKOIJ010000116         8520 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000116 DBKOIJ010000000
VERSION     DBKOIJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.118"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            21..848
                     /locus_tag="ACV5NJ_002113"
     CDS             21..848
                     /locus_tag="ACV5NJ_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486166.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1121..1891)
                     /locus_tag="ACV5NJ_002114"
     CDS             complement(1121..1891)
                     /locus_tag="ACV5NJ_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHY6486167.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            2109..3176
                     /gene="ulaG"
                     /locus_tag="ACV5NJ_002115"
     CDS             2109..3176
                     /gene="ulaG"
                     /locus_tag="ACV5NJ_002115"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HHY6486168.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3194..3655
                     /locus_tag="ACV5NJ_002116"
     CDS             3194..3655
                     /locus_tag="ACV5NJ_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6486169.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3675..5159
                     /locus_tag="ACV5NJ_002117"
     CDS             3675..5159
                     /locus_tag="ACV5NJ_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HHY6486170.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            5202..5501
                     /locus_tag="ACV5NJ_002118"
     CDS             5202..5501
                     /locus_tag="ACV5NJ_002118"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6486171.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5516..6154
                     /locus_tag="ACV5NJ_002119"
     CDS             5516..6154
                     /locus_tag="ACV5NJ_002119"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HHY6486172.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6159..7019
                     /locus_tag="ACV5NJ_002120"
     CDS             6159..7019
                     /locus_tag="ACV5NJ_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HHY6486173.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            7009..7719
                     /locus_tag="ACV5NJ_002121"
     CDS             7009..7719
                     /locus_tag="ACV5NJ_002121"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HHY6486174.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            7951..8385
                     /locus_tag="ACV5NJ_002122"
     CDS             7951..8385
                     /locus_tag="ACV5NJ_002122"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6486175.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         2811 a         1341 c         1818 g         2550 t
ORIGIN      
        1 ttaaagggaa ggaaattgtt ttgattaaag tagatggagc attactaaaa gaataccgaa
       61 agaaaaaggg gttaacacaa gtacaattag cagaaggcat ttgtgaacaa gcaactgtca
      121 gcaatatcga aaacaagaat atgtcgccag gcatcaatat cttaagtgcg ctttgcaaaa
      181 agctgaatgt cgagatctcc gatatattag tagaaacaga agaagacaaa atcaggaaaa
      241 aaataaatga gattcaatca ttgaattctg tatcaaaata ccaagaatct tataaaattt
      301 tacaaacgat cgatcaggaa aaagtaagta attctgatct tttaaatcaa attttatttt
      361 atcaaggact gcttgatcat ctttatgcga aaaaaccaga tgcagcatta ttttatttta
      421 atcaagtgtt ggaacaaacg accgaaacag atatccatca cttacaagca gctgcaaacg
      481 tggcaatgat ctacttgtca aaaggagaat tagattttgc gaaagtttat gtggagcgta
      541 cacttaaaat attgagtgag actgattttg ataatctgat ggtctgcatt gtatattatg
      601 atatcgccac ctattaccgt aaaatcgagg attatgataa agcaatccaa ttatgtgaga
      661 agggtattga gtataataag aaacataaat cgacttatgc attggaatac ttgctttatg
      721 aaattgcttc ttgccataag caattaggag aagatgatta tttagaaaga tatatggacg
      781 caaaaaaaat agcgcgtttc aacggaaatg attacgcggt aaaggtgatt gaaaacgacc
      841 tgaaataaag tggttgtgaa atcagtgttc tgatttgaat accgtttatt cgaaaaaagg
      901 aggtgtgact tttgtcatac ctcctttttt cgatattgtt tgattcctat ttaggcaaca
      961 tataacggtt ataaaagaag caatagatta tgatggtatc gattggatgg atacatcttg
     1021 ggattccttt atcaatttga gcgtagcgtg tttttcattt atcgataaat ttgttattca
     1081 tttttttgat aatcctattt acatatttac gtcctggttc ttaaaatgaa tctacttgaa
     1141 tggtttttat ccccgtattt ctgatctcct ctaatacttt ttcagaacaa aatttatcag
     1201 tgatcaaata atcaaagact gaaatatcag caatcttgaa ctttgatgtc agtccgattt
     1261 ttcgatgatc agcgactaaa atcttacatt tgattgtctt gttgaccatc atttcgttga
     1321 tcgttgattc atttaatatc tttgttgtga ccccatgtac tacatcgact ccgctacagc
     1381 caatgataca aatgtctgca ttgactgaag aaatcgtatt taatgcgatg tcgcccacta
     1441 gtgcttcttt tggaaagcgc atctctccac cagttaaaat ataactacaa ttatctttgt
     1501 gcggagagta attgattttc acgttgtttg taatgactgt caaatggtta gattgaagaa
     1561 aatccagtgt tttcaacgca gtcgagctag aattgataaa cagcgtatca taatcgttaa
     1621 tgtatttact agcttctctg gcaatcgctt gtttaatcct ttcaggacca gattgatcaa
     1681 aatcagtctg gtttttgtaa ttataagaac aataaccgta attgcgtatg atctcgtttt
     1741 tttcttctaa taaatttaaa tctcttcgta tcgtactaat agatacatca agggattcag
     1801 ctaattcgaa agttgatagt tgttcagtct gctgaagcag ttccaaaatt tctttttgac
     1861 gtttttcaat attgacaagt gagtttttca tgacttttgc ctcctgaggg acatcttgat
     1921 ccatggattt atttgactag ttgttcattt aaacattcat atctgtcttt attatactga
     1981 aacaatcatt gattactgaa agaaaatcat ataaaaaaca aacggtcata taaaagggtt
     2041 ttcatggatg ctgatacaca ttaatattta cttgtaagtt ggaacaacga tatgggggga
     2101 gcaagcaaat gagtaaaact gtcaatgaag taacaaaaga aaagtggtta gtagaaacat
     2161 ttcctgagtg gggcacatac ttgaatgaag aaatcgaaac agaagtagtg gatcctggaa
     2221 cttttgctat gtggtggctg ggctgtacgg gaatttggct gaaaagtcat gaaggaacga
     2281 atattttatg tgatctatgg tgtgggacgg gaaaacgtag tcatgggaat ggcatgatga
     2341 aaaaaggcca tcagatgatg cgtatgagtg gcgttcaaaa tatgcagcct aatttacgta
     2401 ctcagccttt cgtaatcgat ccttttgcga taaaaaatgt agacgcttta atcgttacac
     2461 atatccattc cgatcatcta gatatccata cagccgcagc agttcatcaa aattgtcctg
     2521 aagcaacatt cattgggcca aaagaagtgg ttcgcacgtg gcaaaaatgg ggaattcctg
     2581 aagaaaaaac gattacagta aagccaggtg atgcagtcaa aatcaaagac atcaatgtag
     2641 tagctttaga agcgtttgat cggacagcgt tggtcacttg tgaagatcca gaagaggtat
     2701 taaaaggaaa aatgcctcag gagatggatc aaattgcagt aaactattta ttcgaaacat
     2761 caggagggaa tctttatcat gcaggagatt cacattactc caatttattt gccaaacatg
     2821 gtaatgaaca caaaatcgat gtctgtttag gggcctatgg tgaaaaccca cgagggataa
     2881 cagacaaagt tacttctgtt gatatgctgc gcatggcgga gtcattgaat gcaaaagtag
     2941 tgattcctgt ccactacgat atttggtcta attttatggc tgatccaaaa gagatcactg
     3001 aaatttggaa attcaaaaag gatcgcctga agtatggatt taaaccttat atctggcaag
     3061 taggcgggaa attcatctat ccgacaaaca aagatgatct tgagtttaat ttttatcgag
     3121 gatttgaaga tgtcttctcg attgaaaatg atacaccgtt tccgtcgttt ttataagaaa
     3181 gaaggggagt ttcttgctga aatacttttt tgaccatcaa ttgattcatt acccagataa
     3241 agaaatcacg gattggcgtg aagctatcgc cgaaagctgt cttgtattga tcgataagga
     3301 aattattgat gaaacgtatg tggaggagat catccattgt gtagaaaaat acggtcctta
     3361 tattgtgatc gcacctcaag tagcaatgcc gcattcttca gaaaaaagtg atggtgtttt
     3421 tggaacagct atttcattta ccaagttgaa acagccaatc cgttttgaaa gtgaagggga
     3481 gaatgaggag aaaacggcta gtttgttttt tacactcgca gcaaaagatc cagaagaaca
     3541 tctagaaaat atccaaaaat tatccgaatt attaatgaca gatggattgg ttgagcgtct
     3601 catctctacg aactccatcg atgattacca agttgtgatg gcagaatata attaattgat
     3661 agaggagaaa aaagatgggg gaatttttgt taagcatttg gacgtatttt gcaacaaata
     3721 tcttgacgca gccagcatac ctgattggtt ttatcgtgtt attaggctat attttgctga
     3781 aacgcccgct tcatgaatgc atcgctggat tcttaaaggc aacggtcggt tactttattt
     3841 tgactgttgg ctctggtggt ttggtcaata attttcggcc aatcttggtt ggactaaagg
     3901 aacggttcaa tttggatgcc atggttacag atccttattt tggacaaaat gctgtggatg
     3961 ctggattaat ggagacattt ggccgtacct ttggagatgt gatgatttta ttactgattg
     4021 catttgtttt aaatatctta ttagtccgtt ttcagaaata tacgaaactg cgttccgttt
     4081 tcactaccgg aaacgttcag gttcaacaag cagctaccgc tttttggatt ttactgtttt
     4141 gctttcctga tctaggtagg atacaagcat tgatttttat gggactgatt ttaggttgtt
     4201 attggacagt agcttctaac ttaacagtag gaatcacgca ggaattgaca gaaggtgccg
     4261 gatttgcagt ggcacatcag caaatgttcg gaatctttat atttgcaaaa ttagctgagt
     4321 ggatgaaaaa gcgggatgaa aagaaaaata gatcaatcaa gcaagacaaa aaaattgaag
     4381 atatcaaatt accaggattc ttgtcgattt tcaatgagaa tatggttgct acttcgttac
     4441 tcatgctatt tttctttggg atcattctga ttgtactagg caaagattat ttgattcaag
     4501 cgcaattcat gcaagaaggg cagagtttct tattctacat tatgacgact tctttaaatt
     4561 ttgcggtata tctggcgatc ttgcaattag gtgttcggac attcgtcgat gaactaactc
     4621 aatctttcca aggaatctca aacacgattt taccaggagc tgttccagga attgacgtag
     4681 ctgctacttt tggctttggt tcgccaaatg cggtaacgat tggggtcttg tttggtgctt
     4741 tgggacagtt tttgatgatt ggtttactga ttttattgaa atcaccaacg atcgtcatag
     4801 ccggtttcat tccgttgttc tttgataatg ccgttatagc agtttatgcc aataaccgag
     4861 gtggattcaa agccgcttgc ctgttcccgt ttctttcagg aatcatacaa gtaggcggtt
     4921 cagcattatt cgctacatgg attgggatgt caacatacgg tggttatatc gggatgttcg
     4981 actgggcaac agcttggcca ttgtttacag taatcatgaa agtcctaggt tatgctggaa
     5041 ttgccgtagt tgtcattggt ttattggcta ttccgcagtt acaatatcgg aaaaatcctg
     5101 atggttattt tttgattgta gaagactatg acacatatgc agaaaagcag agagtataaa
     5161 agaaaatctg attagacaac actcataatg gagggattaa gatgagaatt ttagtatcgt
     5221 gtgcaaatgg atcaggaaca agcttgatga tgatgagaag tgtggaaaaa gcaatgaaag
     5281 aactaaacgt accaattacc aaaatccatc attgtgccat ttcagaggga aaaagttcag
     5341 ctggccaata cgatgtagtt tttacgccag tcaatttttt gaatatgttt actcaggcag
     5401 agaaaaaagg agttacagtt atcggtatca aaaatgtgat gtcagtcaaa gaaatcagtg
     5461 aaaaatttgc tgaaacaaag tggtaccaaa aaaacaagta aaggaaggaa ctaagatgag
     5521 caggccgaat ttgcaaattg cgttagatca caatgactta gaacatgcat taggagatgt
     5581 tatgaaagtt ggtgatgtag tagatattat tgaagtgggc acaattcttt gcttacagga
     5641 aggacaaaaa gcgattcgct gtattcgcag tatgttccca gataaaaaac tagtcgctga
     5701 tacgaaatgt gcagacgctg gaggaacagt ggcgagtaat gttgcaaaag caggagcaga
     5761 ctggatgaca gtcatttgtt gtgcaacgat tccaactatg gaggcagcac aaaaagaaat
     5821 aggtgaattg caagtagaac tctacggcaa ttggactttt gaacaagcca tggattggca
     5881 taacatcggt atccggcagg taatctacca tcaaagccgt gatgcgctgt tatctggtga
     5941 aacttggggt gaaaaagatt tatcgaagat caaaaaactg attgaactag gctttaatgt
     6001 ttctgtaact ggtggattaa atccacacac attacactta tttgaaggga tcgatgtgta
     6061 tacctttatc actggtcgtg gtattacagc agcaaatgat ccgatgaagg ccgcacaaaa
     6121 cttcaaagat gagatcattc gtatctgggg gtaaggaaat ggctcaaatt gggatttatg
     6181 aaaaagcgtt acccaaagat atttcatgga aagaacgttt ttcccttgta aaagaaatgg
     6241 acttcgattt tatcgagatg tcgattgatg agacggatga gcggttagca cgattagatt
     6301 ggtctgaaga aaagatagct gagctgagag aagagatgtt cagctccggt gtacgtatcc
     6361 attcgatttg tttaagtgcg catcggcgat ttccgtttgg ttctgctgat ccagaaaaga
     6421 aaaaagcagc gaaacaacta atgaaaaaag caatccattt ggcgcataat ctaagcgtca
     6481 aagtaatcca aatagctgga tacgatgttt attatgaaga gaaatcaatg aattccagag
     6541 aagactttat cgaaggaata aaagaaagcg taaaggaagc ttctaaatat ggcatcattc
     6601 tttccgtgga aatcatggat gatccattta tgaactccat ttcaaaattt ttagaaatca
     6661 aagaacagat tcattcacct tttctacaag tatatccaga cctaggcaat ttgtccgcat
     6721 ggccggaaaa taatccggct cgagaacttg agaaagggat cgattatatc actgctatcc
     6781 acttaaaaga tacttaccct gtaacaaaag aatattcagg ccagtttcgt gatgttcctt
     6841 ttggtagcgg ttgtgttgat tttcttggat tattaagaca tttgaaacgt ctagattatg
     6901 atggaacatt tctgattgaa atgtggagtg aaaaaagcca agactttcga agagaaatcc
     6961 agcaggcaaa agactactta agttctaaat tgaaggaggc tggctatgat gtcttgtgaa
     7021 gaaaaaattg cagcaatgaa gcaacgtgtc cttgaagcaa atctggcatt gccgcgatac
     7081 ggattagtca aattgacttg gggcaatgtg agtgaagtgg accgtgagct aggagtcatc
     7141 gtcattaagc caagcggtgt cagatatgaa tgcatgcaag cagatcaaat ggtggtaaca
     7201 gatttgtcgg gaaatataat agaagaagat agcttaaaac catcttcaga tctccccaca
     7261 catgttgttt tgtatcaaac ttttgaagat ataactgcta ttactcatac tcattccacg
     7321 cactccgtca tgtgggcgca agcaggacgc gatttgccag cttatggcac gactcatgct
     7381 gatgcttttt atggtaaggt tccttgtaca cgccaattaa cgaaagaaga agtaagagaa
     7441 gcttacgaag ttcatacagg gaatgtgatt gtcgagacgt ttaaggaaag aaaattagat
     7501 ccaaacgaag tccctggagt acttgtctat ggacatggac cgttcacatg gggggattca
     7561 cctatgaaag cagtagaaaa cagtttgatc ttagatgaaa tttgtctgat ggccaaagaa
     7621 aacgagctga tcaaccccaa tatatgtgaa atcccgcaat atttactaga taaacattat
     7681 tttcgcaaac atggaaagta tgcatactac gggcaataga gatgctaggg taagggtaga
     7741 aaattaaaag ataacacttg atagagagaa acagaggttg tgaagaaaat ttatcacaac
     7801 ctttttattt atcaaaaagt atgcattcta aaaggaaaga ggaattttaa ggaatcgaga
     7861 gaatctttac aggagagtta tatgtgatag attaaatagt caaagaactg tattattaga
     7921 gggagaattg tataagaaag gaagaaaaaa atgaatattg attcccttat gatcgatgag
     7981 atttctgaat tatataagag cgttggttgg acgaattaca caaaagacac tgcaaggtta
     8041 gctagagcat ttgaacaatc tgaatctctg ataaagcgta atggggaagg gaagattatc
     8101 ggaatcgttc gttggatcac tgattttgcg acgatcgcat ttattcaaga tatcctcatt
     8161 catccgaggt atcagcgcca aggaataggc aaagtgcttt tgaatgaagc tttggagaag
     8221 atcactagtt atggaccagt ccaaatcgaa ttattgacag atgatactga gaaaacaaaa
     8281 aaattttatg aatcagtagg atttgttcaa gtaaaggaaa tgggtggtat ttcttatgtc
     8341 atagatacta gaagaaaaaa ggtgcttttt ggtaatgaca aataacatat aataacgtac
     8401 aaaagaaaaa attctagctt agttctttag aaaaacaaga ggatagactt agtatcactc
     8461 aatctatcct ccaatgctca gctattttat ttttttactc acctctttat caaatacaat
//
LOCUS       DBKOIJ010000117         8494 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000117 DBKOIJ010000000
VERSION     DBKOIJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.119"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..149)
                     /locus_tag="ACV5NJ_002123"
     CDS             complement(<1..149)
                     /locus_tag="ACV5NJ_002123"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6486176.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACV5NJ_002124"
     CDS             complement(593..1714)
                     /locus_tag="ACV5NJ_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HHY6486177.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACV5NJ_002125"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACV5NJ_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HHY6486178.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACV5NJ_002126"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACV5NJ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HHY6486179.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACV5NJ_002127"
     CDS             complement(4304..5215)
                     /locus_tag="ACV5NJ_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HHY6486180.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACV5NJ_002128"
     CDS             complement(5232..6239)
                     /locus_tag="ACV5NJ_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HHY6486181.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6557..8365)
                     /locus_tag="ACV5NJ_002129"
                     /pseudo
     CDS             complement(6557..8365)
                     /locus_tag="ACV5NJ_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(8370..>8494)
                     /locus_tag="ACV5NJ_002130"
     CDS             complement(8370..>8494)
                     /locus_tag="ACV5NJ_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHY6486182.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DBKOIJ010000118         8131 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000118 DBKOIJ010000000
VERSION     DBKOIJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.120"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            74..361
                     /locus_tag="ACV5NJ_002131"
     CDS             74..361
                     /locus_tag="ACV5NJ_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486183.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            777..2315
                     /locus_tag="ACV5NJ_002132"
     CDS             777..2315
                     /locus_tag="ACV5NJ_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HHY6486184.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2312..3277
                     /locus_tag="ACV5NJ_002133"
     CDS             2312..3277
                     /locus_tag="ACV5NJ_002133"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486185.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3264..4586
                     /locus_tag="ACV5NJ_002134"
     CDS             3264..4586
                     /locus_tag="ACV5NJ_002134"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HHY6486186.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4649..4753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4827..5570
                     /locus_tag="ACV5NJ_002135"
     CDS             4827..5570
                     /locus_tag="ACV5NJ_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6486187.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5585..6577
                     /locus_tag="ACV5NJ_002136"
     CDS             5585..6577
                     /locus_tag="ACV5NJ_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6486188.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6589..7326
                     /locus_tag="ACV5NJ_002137"
     CDS             6589..7326
                     /locus_tag="ACV5NJ_002137"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486189.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7316..8038
                     /locus_tag="ACV5NJ_002138"
     CDS             7316..8038
                     /locus_tag="ACV5NJ_002138"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HHY6486190.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2681 a         1379 c         1737 g         2334 t
ORIGIN      
        1 ttacttcaag aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat
       61 aaggagtgga aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa
      121 aacagagtat caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt
      181 ccagataaat aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc
      241 tatgaaagac atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc
      301 aaaagaagaa ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata
      361 agtaaaaata agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg
      421 caaaaaagtg tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc
      481 ccacgagatt tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa
      541 aaaagatata gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga
      601 aaaaacgaga accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat
      661 atttagaggt atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta
      721 gttgggaaaa aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga
      781 ataattttat acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac
      841 atctgtccat ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga
      901 tttgggcagc caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc
      961 aaacgattcc ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg
     1021 ttccagcatt gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata
     1081 ttggtctagc tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca
     1141 gaatgagacg cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta
     1201 tccgaactgc cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag
     1261 gtggattagg gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga
     1321 ttggtgcgat cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc
     1381 agcataagaa acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg
     1441 gcgtttcttt atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat
     1501 taggagcgga acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa
     1561 aaacagaagt tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg
     1621 atcaaggtca aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga
     1681 aggtacctga agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag
     1741 aagaattatt gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata
     1801 cgtatgcctt ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aagatttccg
     1861 atttggcagc tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt
     1921 cagatggata taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca
     1981 tggagccaag tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct
     2041 attccacaga tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt
     2101 tattcccagc ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg
     2161 aaatcgttaa agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata
     2221 tgaattacca agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga
     2281 aaaatgaagg attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt
     2341 gcaaaaaaag tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg
     2401 agaaatcttt gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat
     2461 caatggatta tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga
     2521 atataatctg cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt
     2581 cccgaccatg acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa
     2641 gcagaaaaga gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat
     2701 ttatcgagat cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat
     2761 ccgagctatt gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga
     2821 tccgatttca agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac
     2881 gaccatcgtt ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc
     2941 atttatgaag gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc
     3001 taaaaacgac tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt
     3061 agaagattta gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca
     3121 agtttgcttt gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat
     3181 tgtgatagta gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt
     3241 tttaagtcga gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg
     3301 tgagcggctt aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg
     3361 aagaagatct atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt
     3421 taagtgcagt agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga
     3481 tcccaactgc caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc
     3541 cagaaagccg aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc
     3601 gttttttgga tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag
     3661 ttttggcaac cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa
     3721 ctagtgcaga ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg
     3781 gttatattgc ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg
     3841 tccatcataa tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc
     3901 atcaaatgga agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt
     3961 cagaaggtac tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt
     4021 tttgtgcgac tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg
     4081 tctttgataa acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt
     4141 ttgcgtgtgg agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg
     4201 aaggaaatta tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga
     4261 ttatacctac gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc
     4321 acgcctcttc gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc
     4381 gtgtcaatga accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg
     4441 gtgctgcagt gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca
     4501 atcaaaaatt gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa
     4561 gtgatctctc ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc
     4621 cttcttgtgc tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg
     4681 agagtaagtt gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa
     4741 catagcagac gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt
     4801 catattagaa gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg
     4861 cattcatttt aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt
     4921 tacccggacc tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc
     4981 attctgtcac gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt
     5041 ttattactgc cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac
     5101 tagtgatttc acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg
     5161 gtttcggtat gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag
     5221 tttctttttc ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga
     5281 tgggggcaga tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct
     5341 tcttttccgg tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat
     5401 ggctaagcgc tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga
     5461 tcgataaagt ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg
     5521 cttgttcatt attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga
     5581 tcaaatgaaa aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac
     5641 tgcaaaagaa acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt
     5701 ggattatgta ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta
     5761 taaagaggct ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg
     5821 tctagtaggt gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc
     5881 tcacgcagat ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac
     5941 ttctggattc gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa
     6001 aacatatgca ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa
     6061 agacggcggg gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag
     6121 cttaggaaaa tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa
     6181 agaagcgggt attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta
     6241 ctatactccg gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca
     6301 gtcttttatg gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag
     6361 tgctaaactg ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca
     6421 agcattcttg tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa
     6481 agtttggcgc aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc
     6541 ttcgcaagaa ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa
     6601 atcagccact tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact
     6661 atccctaaag atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg
     6721 ctgaaaactt taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa
     6781 aaagtaactg gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct
     6841 tggctgagcg tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat
     6901 gaagaacaag taagggatta tctggatatc tttgggttaa acggctatga acattttttg
     6961 ccggaacaat tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat
     7021 ccttcttctt acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg
     7081 cagatgcaag actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg
     7141 acgcatgata tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt
     7201 ccggcacatg tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta
     7261 ctgcagcaat cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt
     7321 aagtgatgct cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca
     7381 cacgattttg acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct
     7441 tgcagcaagt aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta
     7501 tacgtttgaa caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt
     7561 agataatatt tggacgaatg taccgatgac tacacaaaaa aaagtatttg agcgacagct
     7621 tgcttttgca gccataaatg agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga
     7681 gatcttaaat tatatccaag cttaccctaa tcactatttg atccattggt attcttcttt
     7741 ggactatcaa caagactata ttgatttagg ctgttatttc agcattggtc ttgatttgaa
     7801 aaagaatcca gcagtctggc aactagctaa agcagtccca atcgaccact tacttattga
     7861 aacggatgga ctacatgcag cagagtgggc tttagggggg attttttcag aaaaagatta
     7921 tgtctcactg cttgaagaac agttaaagac aatcgctgag atcaaagagc tatcagttga
     7981 agaagttaaa aaaaatactt atcaaaactt ggaacgtttc attagattag gtaaataaaa
     8041 aagagatgtg acacaactag tgtcgcacct ctttttggct ttctttgtat aaaagtaaac
     8101 tgtttgatta ataaatgaat cttagtacca a
//
LOCUS       DBKOIJ010000119         8067 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000119 DBKOIJ010000000
VERSION     DBKOIJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.121"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(133..589)
                     /locus_tag="ACV5NJ_002139"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACV5NJ_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACV5NJ_002140"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACV5NJ_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACV5NJ_002141"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACV5NJ_002141"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HHY6486191.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACV5NJ_002142"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACV5NJ_002142"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HHY6486192.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACV5NJ_002143"
     CDS             5704..6489
                     /locus_tag="ACV5NJ_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHY6486193.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACV5NJ_002144"
     CDS             6502..7353
                     /locus_tag="ACV5NJ_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HHY6486194.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACV5NJ_002145"
     CDS             complement(7477..7929)
                     /locus_tag="ACV5NJ_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486195.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2714 a         1372 c         1710 g         2271 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaat
//
LOCUS       DBKOIJ010000120         8031 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000120 DBKOIJ010000000
VERSION     DBKOIJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.122"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            107..376
                     /gene="rpsN"
                     /locus_tag="ACV5NJ_002146"
     CDS             107..376
                     /gene="rpsN"
                     /locus_tag="ACV5NJ_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HHY6486196.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            391..570
                     /gene="rpmF"
                     /locus_tag="ACV5NJ_002147"
     CDS             391..570
                     /gene="rpmF"
                     /locus_tag="ACV5NJ_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HHY6486197.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            602..979
                     /locus_tag="ACV5NJ_002148"
     CDS             602..979
                     /locus_tag="ACV5NJ_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486198.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1000..1149
                     /gene="rpmG"
                     /locus_tag="ACV5NJ_002149"
     CDS             1000..1149
                     /gene="rpmG"
                     /locus_tag="ACV5NJ_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HHY6486199.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1173..2156
                     /locus_tag="ACV5NJ_002150"
     CDS             1173..2156
                     /locus_tag="ACV5NJ_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HHY6486200.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2186..2308
                     /locus_tag="ACV5NJ_002151"
     CDS             2186..2308
                     /locus_tag="ACV5NJ_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HHY6486201.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2326..3849
                     /locus_tag="ACV5NJ_002152"
     CDS             2326..3849
                     /locus_tag="ACV5NJ_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HHY6486202.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKTKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3873..4112
                     /locus_tag="ACV5NJ_002153"
     CDS             3873..4112
                     /locus_tag="ACV5NJ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486203.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4305..4766)
                     /locus_tag="ACV5NJ_002154"
     CDS             complement(4305..4766)
                     /locus_tag="ACV5NJ_002154"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HHY6486204.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4961..5179)
                     /locus_tag="ACV5NJ_002155"
     CDS             complement(4961..5179)
                     /locus_tag="ACV5NJ_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486205.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5297..6913
                     /locus_tag="ACV5NJ_002156"
     CDS             5297..6913
                     /locus_tag="ACV5NJ_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HHY6486206.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7154..>8031)
                     /locus_tag="ACV5NJ_002157"
     CDS             complement(7154..>8031)
                     /locus_tag="ACV5NJ_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HHY6486207.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT         2751 a         1248 c         1618 g         2414 t
ORIGIN      
        1 tatttattgt tgacaagagc aactcttgtt ggtaaagtat cagtgtcaaa acgtaacgat
       61 tacgatttga ggtgtctaaa tcaaaataaa aggagtataa aggtatatgg caaaaaagtc
      121 gaaaattgct aaagcaaaga aacaaatggc gatgattgaa aagtatgcag ataaacgtca
      181 ggaattaaaa gccgcaggag atcgcaccgc tctcgctaaa ttgccaagag attcaaatcc
      241 gaatcgtctg aggcttcgtg atcagacgga tggtcgtcca agagggtata tgcgaaagtt
      301 cggtatgtcc cgtattaagt ttagagaact tgcccatcaa gggttgattc ctggagtaaa
      361 aaaagcaagt tggtaaaagg aggaaatatc atggcagtac cagcaagaaa aacatctaag
      421 gcgaagaaac ggttaagaag aactcatcaa actgtagtaa aaccagaaat ttcatttgac
      481 gaagcgaatg gtgattatag acgtagccat catgtatctt taaaagggta ctataatgga
      541 aagaaagtaa ttaaaggagc ttcaaaatag tttaatatat aacttttagg agggaatcaa
      601 aatggaaatt atttacccac ctttagttga agaaggatta aagtattatc ttgaaacgac
      661 ccagcaatcg ctagataaat gcacttttta tcgttcgatg gtagagagag gaattattac
      721 tgaaacgggt ttaccgaccc aacaggcaat tgaaaatgga ttagtcaaag attactatga
      781 ggaccaaggc ttatcatttg atgatttttt aagaatctat ccaatttttg aagagtatga
      841 cgaggagtta tttcaatgta tcgatggtta ttgggagata cctgttgata tgaaagaaaa
      901 tttagtttca caattggaat ctggcgagtt aacttttgaa gatgcacaac aaattcaagc
      961 atatcttgaa gatcgataag tattgaagga ggaagattta tgcgtcaaaa tattattttg
     1021 gaatgtgtcg aaactggaga acgtctgtat ctcaccagca aaaataaacg gaataaccct
     1081 gaacggttag aattaaaaaa gtattcgcca aaattgcgca gacgggcaat ttttaaagaa
     1141 gtgaaatagc taagtggtaa aaaggtgaca aaatgggcat accagttaca atcgtttctg
     1201 ggtttttagg ggctggaaaa acaacgctaa tcaatcaagc cttgcaagtc tcaccctacc
     1261 ccagggaaga aattattata attgaaaatg agtttggtga agttgggatt gatcacaaat
     1321 tattagttca tagcgaggaa cgggtgttac aactaaataa tggatgtatg tgttgcagtc
     1381 ttcgcagtga tttattagca atattgattt ctttactgga aacaatagaa gaacatcaac
     1441 aaccaatttc acaaatcatc attgaaacta caggtatagc agatccccaa ccaatcattc
     1501 aaacactttt aacagcgcct caactaagag gaagagtata tatcgatagt ttattgactg
     1561 ttattgatag tgacagtttt gaacttattg aaagagaacc acaagccctt aagcaacttg
     1621 tcatggcaga tcgcattttt gtgtctagaa aacaaaactt gaatttatct gcactaaaaa
     1681 aattacagag agaaatcaaa agtattaacc ctttatccga tattcgaatg ttcacacata
     1741 gtgaaacgat tcaaaaaaag gatttttata atttgaaaaa atttaatcat cccttagttt
     1801 ttgaaagtga ttcatcagaa aatcactata atggcaaata ctatcatcaa caccatcaag
     1861 tacatgagtt taaatcaatt ctcttaactt cagatcaaga cataaaaaaa gattttttac
     1921 tccaatggat tgactggctg attttaacca accaagggag cctgtatcgt tttaaaggat
     1981 tgattgcttt acaagagcag gattttatag ttgccatgca agaggtaaac gaacaggtta
     2041 attttcaata caccactcga caaaaagacc aaaccactat tattctaatc ggaaaaatgt
     2101 tggatgaaga aaggatcaaa cagagttttg ataaattact ggcggaaagc aaataatgat
     2161 aagtagtgat tgggggtaat ttctaatgga gaaaacggat atatctagtg catatcgacg
     2221 acttaaaagc ccgaatatta aaactagaaa aagggcatta aaaattatca aagaacataa
     2281 acgaaataaa atgaagaaat tagcataatt aaggaaggaa aaagaatgaa gaaaatcagt
     2341 gtaggactta ttgggatagc agctctagga ttattgggag cttgctccag cactaatgat
     2401 gcaaaagtat cgaatgataa agatggaaaa ttagaaattg taacaacatt ctatccaatg
     2461 tatgatttca ccaaaaacat cgtcggcgat gaagcgaacg tggatttaat ggttccagcg
     2521 ggatcagaac ctcatgacta tgagccctca gctaaggata tggcaaaagc tcatgatgct
     2581 gatgtttttg tatatcataa cgaaaacatg gaatcatggg taccaaaagc taaagaaagt
     2641 tggaagaaag ctgggcctaa tgtagtagaa ggaacaaaag acatgatttt attgccaggt
     2701 agtgaagaag aagatcatga tcacggagaa gaagatcacc atcatgaact agatcctcat
     2761 acgtgggttt cacccaaaat ggcaattaag gaagtttcaa atatcaagga tcagttagtt
     2821 aaattgtatc cgaaaaaaac aaaagtattt gagacaaatg ctgaaaagta tttgactaaa
     2881 ctgaaacggt tggatgctga ctataccact agtcttaaag aagcgaaaca aaaaagtttt
     2941 gtgacgcaac acgcagcctt tggatattta gctttggatt atggattgaa acaagttcct
     3001 attgctggac taagtcctga agaagagcca tcttctggtc gattagctga attaaaagag
     3061 tatgttaaaa aaaataagat caattatatt tactttgaaa agaatgctaa tgataaaatt
     3121 gcaaaaactt tggccaacga agctgggata aaattggaag tactgaatcc tcttgaaagt
     3181 cttacaaaag aacaaatgga taatggtgaa gattatgttt cagtaatgga agacaatcta
     3241 aaagcgttag aaaaaacaac tatggttgct ggtgaagagg tagttccaga aaaagaagcg
     3301 aaggatgaaa agacagttgc taatgggtac tttaaagatg ccgatgtgaa ggatcgcgaa
     3361 ttgagtgatt atacaggcga atggcaatct gtttatccgt tgttaaaaga tggaattttg
     3421 gatgaagttt tcgattacaa agcaaaaatc aataaagaca tgacagctgc cgagtacaaa
     3481 gattattata ctactggcta taagacagat attgatacta tcaacattaa agacaacacc
     3541 attgattttg tagtcaacgg tgaacatcat caatacacat acaaatatgt tggatacaaa
     3601 attttgaatt atgaaaaagg aaaccgtggt gttcgtttta attttgaaac tgatgatgca
     3661 ggtgctggtc gttttaaata tattcaattt agtgatcatg gaattgcacc aagtaaggca
     3721 gagcatttcc atattttctt tggtggagaa agtcaagaaa aactctataa tgaattgcat
     3781 aattggccta ctttctatcc agcttcattg tctaaacatg aaattgctca agaaatgatg
     3841 gctcattaaa attcaatttt tggaggaaat acatggtaag aaaagtatta ttgtctatgg
     3901 attccaagaa tattgatgct tatcggcgtt atcgatttgc aaagcaaatg agattaaatg
     3961 ggagtacatt agctgatatt gctgcaactt tacatgttag tattgacaca gttcatagag
     4021 tattaaaacg ggatcctgat tgttgtttta tcaaggtcaa tactggaaga agtagacgtt
     4081 tagcaaaacg gcataatagc ttggccaatt aattgataat accagagtag atcatttttc
     4141 ccttatttgg atttgatctg ctttttatta tgtcttgaat tgtcaaatca agcgcaacac
     4201 tttatcaaag aagacttcat aaggtgttct ccaatctaaa cactttctag gtctgttatt
     4261 cagtttttca atcatctgat ttatcgttga gttatctacc gtagctatct ctttcgattt
     4321 tggcaagtat tctcgaatga gtctattcgt attttaattt gttcattgtt gccaagggca
     4381 tgaggatctg gaaaataaaa gggcatatta ttcaatgctt ctgtaactgc gaaatgattg
     4441 gagaattctt ttcctctatc tggagtgatc gttttgaccg cctcatttgg aatactgctt
     4501 aaaagttcaa tcattttctc gctcacataa gcggctttct ttctggggac cttctttcct
     4561 gctaaaagga agcatgattt tctgtctgtc aaagtcacta aacaagtacc accagttttg
     4621 ccagctacaa tatcggcttt ccaatgacca aaatcacttc tatcatctgc ggattttgga
     4681 cggccatgga tcgaatgagt aaggcaaatc tttccacgag actcgacatg attttttgta
     4741 tgtcttgttt ttcctcgatg gtgcaatgaa cggatcaggc ctttattgcc gtgtgagagg
     4801 ggttctgtat cgaaaagatc attgcaaatt gcacgttaga tcgtgataaa actaattgaa
     4861 aagtccatat gttcatgctt caaacgattc gagatttcct ctggcgacca ctgaaaatcg
     4921 acaaataaat gacgaacaat tgaccaaact cacggatcat ttagaatgtg ataacgtccg
     4981 catttttttc ttcgataagc ataatttttt tccgctttgg aaggtgaata gtttttttct
     5041 tgtttcttct cagttcatgt aaaatagtaa acgggctatg ctgaatgtcg ttgccaatta
     5101 tactaataga aaatccttct tcgtgaagca gaaagattct ttctcgttcg agtatagtaa
     5161 gatgacgata ctggctcata attattttcc ttctaggagt gttctcgaca aacttatact
     5221 agttggatat cgattatgac tcatttttat gtgttgcact taaagtgtaa attcaaggta
     5281 tagaaagagg ttccctatga aaaaaatacc tattacaatc ttaactggct atcttggttc
     5341 aggaaaaacg atacttctaa atcatattct gtacggtgat cacggaaagg aaattgctgt
     5401 cattgtaaat gacatagggg aagtaaatgt tgatggggta ttagtcgaca agaaaggttt
     5461 taaaagaact ggagaaaaac ttgtttcgat gccgaatgga tgcatttgtt gtacattacg
     5521 cgaagatttg attgttgaac tagatcaatt agtgcaattt tctgacttga atcaaattgt
     5581 tattgaagct tcaggaatca gcaaaccaat tccaattatt caagcaatta tattagctaa
     5641 aaacccttta gacattgatt tgacccaacg attagagatt ggtgccatta ttatagtagt
     5701 ggatgtaaaa cgtttttggg ataatttcga aacgggtgaa aagattcagg aaagaaaagc
     5761 tgaaggtaat gtagttgaag aagaacagga tattcgtggc ttgttaattg atcaattcga
     5821 gtgttgtaac atattacttt taaataatag tgacttagtg tcaaccaaga cgatttacaa
     5881 aattaacggt ctaattgcta agttacagtc caatgccaaa ataattagga caattaaagg
     5941 tgaagtagac ttatcgaaaa ttctaaatac aaatcttttt aatatagatg agacttccta
     6001 ttcagtgggc tggttcaaag agttagagct tggctatcaa aatcatattc ctgaaacaga
     6061 agaatatggt atctcaagtt ttatctatcg aaatcggtta ctccttgacg cacaaaaatt
     6121 tgcccatctt acggataaaa attttcctgg caatattaca agagcaaaag ggtttctttg
     6181 gtttgcccaa catagtgatt tttcgacgat ctttgaaaca tcgggtagct ataaaagtat
     6241 tgaatcatta aattattggg tagctagttt acctaaaatt gagtgtcaaa tgattataca
     6301 aaaaaatccc gaattgaagg aagcctggca tccgttgttt ggtgaacgca ttaataaact
     6361 agtttttatt ggtatgaaga tggatcaaca atgtattaag gaacaacttg agggttgttt
     6421 aactgattct cttaatgcag ataatttccg aaaaatttca ccatttccat ggaaaaagtc
     6481 taatatgttt agccagatat cgttaatgaa atttttaaga aaagctggtg tcaagatagg
     6541 cgatgtcata attattggca ttttgattat tacctctttt attcccttat ttgtattctc
     6601 tatagaaaag gagaatgaaa caactgaaga tgctatcaaa tatgccatcg ttcgaattga
     6661 tggcaaggaa atggatcgct tcgatttgga taagatcaat cataaactcg tgaattatca
     6721 tcctgcgaag ggacaatata atattgttga gattaaagat ggaagaattc gagttaaaga
     6781 agataatagt cctgatcaaa tcgcggttag aactggatgg atctcaaaac ccggtcaaac
     6841 gagtatttgt ttaccccata agttagttat tagtattgag aaaaaagaaa gcacagatta
     6901 ctacatttac taatctggaa atagatgagg aggtttttaa taagaaaaag atattattat
     6961 taataatagc aggggcttta ttaagtgtgt taagcgcttg tggacaagaa aaagaagcgg
     7021 tgacaacagg cacaagtatc tttaacggga cctgcaatgg tttagttaac agtcggttag
     7081 attacttatt ttggctcttc gtcaatgagt gttggtgaag aaattcaata gaataatcga
     7141 gatactcaca cggctagaca gctatcttgt ctagccgtgt tgttgttctg gctttagtca
     7201 gttgggtagt attcttagtt ttcttaaaat attttcctcg atataaaaag attcgttctc
     7261 ggtagttttg aaagtttcta tagccatagg caattcgttt tagtactttg atatggttat
     7321 tcaaacattc tatagggccg ttggaatagg gaacgcgcaa cgctcgtttg atttctgttt
     7381 ggtatttctt gaaggtcgtg aacactggtt ggtagtgctc tggcaactcc ttgtagtctt
     7441 cttttaggag cgcgacgaat tcaggaacat cttgtcttct gataacggaa agaaaatcct
     7501 ggtagacttc gtagcctcgc tttaaggaat catcataaga taatagccga tcgactatct
     7561 ctgcttctgt cagatgatct cgaaatgacg gacgccagta gcgatgctga cctttcaatt
     7621 cccacgcatt cttttgaagt agtttccagt atttctttaa gtgcttcgac cgacgatccc
     7681 cttttttaaa ggtcttcatt acttgaattc ggtggttggt aaaagcccga ccaatgtgtt
     7741 ggctaatatg aaagcgatcg gcgacgattc gggcattcgg aaaacactct ttcaccaatg
     7801 tgcgataggg tgtatagaaa tctgaaacca ctagacgcac ttgttcacgg acttttatag
     7861 ggtagcgcaa gaaatagtcg cgtaaaggat tgagttgccg attttccaca atatcaatca
     7921 gttgatgggt cttgccgtcc atcatgacaa agctcatggc tcccgagaca tttttcactg
     7981 atttgaattc atcgaaacac accacttctg gtaacagctg gctcaaagaa a
//
LOCUS       DBKOIJ010000121         8005 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000121 DBKOIJ010000000
VERSION     DBKOIJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.123"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            244..2010
                     /locus_tag="ACV5NJ_002158"
     CDS             244..2010
                     /locus_tag="ACV5NJ_002158"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HHY6486208.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2112..3371
                     /locus_tag="ACV5NJ_002159"
     CDS             2112..3371
                     /locus_tag="ACV5NJ_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HHY6486209.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3518..4819
                     /locus_tag="ACV5NJ_002160"
     CDS             3518..4819
                     /locus_tag="ACV5NJ_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6486210.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4822..5673
                     /locus_tag="ACV5NJ_002161"
     CDS             4822..5673
                     /locus_tag="ACV5NJ_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HHY6486211.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5686..6501
                     /locus_tag="ACV5NJ_002162"
     CDS             5686..6501
                     /locus_tag="ACV5NJ_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HHY6486212.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6652..7818
                     /locus_tag="ACV5NJ_002163"
     CDS             6652..7818
                     /locus_tag="ACV5NJ_002163"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHY6486213.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2605 a         1375 c         1596 g         2429 t
ORIGIN      
        1 tgttaaaaaa cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga
       61 cactaaatag tacaggaagt agattaaaac ttttaaaagt tttaagtcta tctatttggc
      121 aagtttaaaa aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg
      181 ttgtcttttg tttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa
      241 tcaatggata cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg
      301 aatacaagat ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg
      361 ctttgtggag atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg
      421 aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca
      481 aggcgcttac agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt
      541 gatcaaggaa tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga
      601 ataccttatt ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta
      661 tggtatcaaa tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac
      721 actttacctt ggggaagtaa agatccagac cgagaagact tcttcggggg agatttgcaa
      781 ggtgtgaggg atcatttgga ttacttgacg gaccttggcg tcaatggcat ctatttttgt
      841 cccatattta aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat
      901 cccgcttttg gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg
      961 atacgtatca tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa
     1021 gacgttctca aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc
     1081 ccagtagatt cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat
     1141 acttttgctt ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa
     1201 tatttgctgg atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt
     1261 gatgttgcta atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa
     1321 atcaaaccag acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa
     1381 ggggatgaat tccatgcagt gatgaattat gcattcacag attcgattaa agattatttt
     1441 gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt
     1501 tacagagatc aggtgaacga aggaacattc aacttattag attctcatga tactgccagg
     1561 atcttgaccc tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt
     1621 cttcaaaaag ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat
     1681 gacccggatt gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc
     1741 ggttttataa aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt
     1801 tctactcagt ggctaatagt atatgatcgt gaagataagc tctatttcac acgggaacta
     1861 gagggacaga tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag
     1921 gaaaatgagg tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata
     1981 cgacaatacg gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt
     2041 ttgcaaatga agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacacagg
     2101 aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag
     2161 gaatcttagg cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg
     2221 atgaaggaaa aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa
     2281 taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt
     2341 tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga
     2401 tggcagcata tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac
     2461 tgggaaatga agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa
     2521 tctacggaga acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg
     2581 atacagcacc atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt
     2641 ttgagtcaga agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt
     2701 cctatggttt aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt
     2761 cagatatcgg tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt
     2821 tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc
     2881 agcaattcat gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat
     2941 acaaagaaaa caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc
     3001 cttatcaacc gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag
     3061 acctctctca aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg
     3121 aaatggtaaa tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg
     3181 atgaattaac aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc
     3241 cagaaatggg cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt
     3301 caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac
     3361 aaaaatactg attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag
     3421 ataaaagtgg caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag
     3481 cagtttgcgg tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa
     3541 tatcaacgaa aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa
     3601 tggccaagct tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat
     3661 gcttctgggc ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga
     3721 agatcattca ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt
     3781 cttgattttt tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga
     3841 gggcaaaaaa atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt
     3901 tccttatcta ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt
     3961 cctagttaca ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag
     4021 tttaattgat tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg
     4081 taatactttt tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt
     4141 gcaaatcacc cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa
     4201 acgtattttc ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat
     4261 gagtttttcg aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt
     4321 cataaaccag cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg
     4381 gacgaaagta gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat
     4441 ggttacagga atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg
     4501 agcaaatgca tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc
     4561 gccaacattt attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt
     4621 taacaatggt ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc
     4681 ctggatctat aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac
     4741 gttgattatc tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc
     4801 gttcaatatg ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga
     4861 ctttctcact tttttactta cttttttctt ttaatactgt cagtcattat catctatccg
     4921 cttttgatca cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat
     4981 ctttcaagtg actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg
     5041 acttggtata aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta
     5101 gtaactttgg cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg
     5161 atgttctttt tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc
     5221 atggctttct tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc
     5281 ggtggaattc caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat
     5341 ttagatgaat ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta
     5401 ttaccgcttg tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt
     5461 ggggatttca tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc
     5521 ggcttacaaa catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg
     5581 gcaattttga ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt
     5641 accggcctga catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg
     5701 ttaaactatt tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta
     5761 tcatggccta aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg
     5821 actctttttt atgccaatca gatccaagcg attccattag aacaatttct atccgttcat
     5881 tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg
     5941 ttacaagcat cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta
     6001 tctgagaaac aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg
     6061 atccaacaaa aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc
     6121 caaccagatt ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat
     6181 gccagtaatc gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt
     6241 gtcatgacga gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc
     6301 caactttcat ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga
     6361 ataggttcca tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta
     6421 agtgtccaaa caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc
     6481 aaagataagc gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa
     6541 atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct
     6601 tttctttttt agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag
     6661 aaaactttat acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat
     6721 tgggacggta caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat
     6781 atccatgaag aaatcccaat gaaagagatt acaagtaacg cttctttggc actaggagaa
     6841 gcctatatgg atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct
     6901 tatcagcaag caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa
     6961 gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac
     7021 gacttttata aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca
     7081 cctgaagata cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc
     7141 tttatcaaag aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc
     7201 actgctacca aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac
     7261 gatttgatta ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta
     7321 atggattacc gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc
     7381 gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa
     7441 ccaaaaggaa cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct
     7501 tggatcaata aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat
     7561 aatattacaa aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa
     7621 ttgacattag aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa
     7681 atgggtgatt ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt
     7741 gaatcaagta atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc
     7801 ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct
     7861 aagttttatg atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag
     7921 cttagtttct ttttctttat attttcataa taaaagtttg gttctttaat ttttaaacat
     7981 gacagaaaat tttttcttcg ttata
//
LOCUS       DBKOIJ010000122         7959 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000122 DBKOIJ010000000
VERSION     DBKOIJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.124"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            26..211
                     /locus_tag="ACV5NJ_002164"
     CDS             26..211
                     /locus_tag="ACV5NJ_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HHY6486214.1"
                     /translation="MNYKWILCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIK
                     QFKVTVITEPDAKTQSR"
     gene            complement(271..624)
                     /locus_tag="ACV5NJ_002165"
     CDS             complement(271..624)
                     /locus_tag="ACV5NJ_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486215.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1128..1547
                     /locus_tag="ACV5NJ_002166"
     CDS             1128..1547
                     /locus_tag="ACV5NJ_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HHY6486216.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1573..1779
                     /locus_tag="ACV5NJ_002167"
     CDS             1573..1779
                     /locus_tag="ACV5NJ_002167"
                     /inference="COORDINATES: protein motif:HMM:NF024059.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486217.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2007..2180)
                     /locus_tag="ACV5NJ_002168"
     CDS             complement(2007..2180)
                     /locus_tag="ACV5NJ_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486218.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            2192..2821
                     /locus_tag="ACV5NJ_002169"
     CDS             2192..2821
                     /locus_tag="ACV5NJ_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HHY6486219.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(2903..4069)
                     /gene="dnaJ"
                     /locus_tag="ACV5NJ_002170"
     CDS             complement(2903..4069)
                     /gene="dnaJ"
                     /locus_tag="ACV5NJ_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HHY6486220.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(4220..6049)
                     /gene="dnaK"
                     /locus_tag="ACV5NJ_002171"
     CDS             complement(4220..6049)
                     /gene="dnaK"
                     /locus_tag="ACV5NJ_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HHY6486221.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(6100..6663)
                     /gene="grpE"
                     /locus_tag="ACV5NJ_002172"
     CDS             complement(6100..6663)
                     /gene="grpE"
                     /locus_tag="ACV5NJ_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HHY6486222.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(6757..7800)
                     /gene="hrcA"
                     /locus_tag="ACV5NJ_002173"
     CDS             complement(6757..7800)
                     /gene="hrcA"
                     /locus_tag="ACV5NJ_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HHY6486223.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
BASE COUNT         2235 a         1648 c         1423 g         2653 t
ORIGIN      
        1 aagtgtaaag gaggagatag ttattataaa ctacaagtgg atattgtgtc ctgtatgtgg
       61 aaataaaaca cgattaaaga taagggaaga tactgaatta aaaaaattcc ccctctattg
      121 tccgaaatgc agacaagaaa atttaattga aataaagcag ttcaaagtaa ctgtgattac
      181 agagccagac gcaaagacgc agagccgata aaatgagatt aatacaatct cattttatcg
      241 gctctttccg ttatgtatgg attcttttaa ttagtcttcg atgtttcttg cttcgttgat
      301 accgctggct aaagattcca ttaaggatag ttctttgtct gtaaagctat ccatgtattt
      361 ctctatctgt aatcgtcggg tgctttttac caagttatta gcaggtaaga aaaattcatc
      421 aacggaaaca tgaagtaacg atacaaggtc ataaagaact tgtatgctgg ggtgttgccc
      481 tttattttca atattagtta agtaccgtgg gtcaatttca atcaatgctc ccacttgttc
      541 acgagttaaa cctcgtttca atcgagcttc tttaatggct aaaccaaagg ctctaaaatc
      601 atatttatct tcttttttac gcatagtaga ccacctctat acattttatt gttcctactg
      661 aattaaaaac aggtatagaa aaacgtgtta tatggtttat aggtttatat ttaataaaaa
      721 gcactactaa acgccaataa aaaaaaccgt tatatggtag tgctatttac gctgttaaaa
      781 tattgtatat tacttccaaa tggcggtttg ttggaggtca acgtcgccat gaagtacatc
      841 atatacaata aatttcctta cattgggttc ttgtcaaaaa aagtcgtcta tctgcaatag
      901 ataagtacgt ccaccaatgt ggttttataa atcatataga tagaataaca gaagcatgta
      961 aacagagaaa taaatctgtt tatatgcttt tttggctatt cagaactttt ttacaaagtt
     1021 tatttatcag taatgcaaca aatccccctt tcacattggg actaagagtg aaaggagata
     1081 aacgagcaag gctcacttcc tttcctagac agaaaggggg tgagattatg aggacatctt
     1141 ctttcgagca tatcgtcaga ttacagttca attctttaat gctgattgtc attaagaaca
     1201 aagtgaaaag tcgaaacaga cagcttgcaa gacattctaa acgtgaagta ttgttttgtg
     1261 aaatagccga aacgaaacaa gttgaacgtg gaacgattga tacctgtttt tatgattgtg
     1321 tatcatttaa agtcttgcac tttacaattt atgtatctga tgaaacactt ggtacagttt
     1381 tacatagact atcagaaaaa cagcgtagtg cgattttatt acgttatttt caaggaatga
     1441 acgacagaga aatttcagaa ctgtaccatg tatcacgttc tgctatttgc agtagaagaa
     1501 ctagaggact aaggaagtta aaaatgcttt tgagcgaaag gaagtaaaga ttatggctat
     1561 ccctcttttg aattgatttg caaagcgtca agcggagatg aaatggcagt taaagagatt
     1621 ttgaagtttt atgacggtta tatttctaaa ttatgcttgc gtccttttta ccactctgaa
     1681 agtggtaaaa tcattatgca ggttgatgaa gaactaaaag gagaaattca tacagacatg
     1741 atgaaagcaa tattgaaatt tgaaatcgga gtgaagtaat caactgtata aaaaaatcgg
     1801 gagatacaca gtttcgtgtt atctccctta gtagttttac ggtcatatca aggctcattc
     1861 aatcgtggta aattcgtggt aagatgagtg tttttctata cttcaaaatg cttgaatgta
     1921 tagtgtttat gcggataatt gaaaattaga tataaaattt aataaaaacc aaaaatgcac
     1981 ggtcgccatg aagggaccgt gtatttttat cttcttattt tctcacctaa tcgtatcagc
     2041 cgcatcgctt gttttcctgc acaaacaagc aatcgctcta cctgttccat cgcataagaa
     2101 agtaccatcg gttcattgat gatatcaagt acatagtcga taccaccctc acttaattga
     2161 gcttgatttt tcttctccat attatgtagt tgtgggacaa ctttctgaca atcagcaatt
     2221 gtgtcaagaa cggatcaatc ctttattgga gttgctcgaa agggtcttct cttattacgg
     2281 acaggctttg ctcactgtac atagacaaca aatcattatc ctagtcaatc ggatttccag
     2341 agcctcattg ctatcattgc ttgataaaat ccagacaaag tttaataaaa tgtatcagtt
     2401 acagctaaac tttggaattg ggtcgctttg ttatacagag caagagacac cgcaatcttt
     2461 ccttcatgcc aaacaagttt gcgaatggat agctttccat caatctgtga acgagatccg
     2521 cttttttgaa gatttagacc tcgggatcgt tttaccagca atcccgtcag atcaacgaac
     2581 attatatgta aaacgtattt tgaaaagcct gacagaagaa gaagtacatt tgttcaagaa
     2641 gactttggca tgcttcagta aaaataacgg aagcatcaaa aattgctcag aagaattatt
     2701 tattcataaa aacaccttgc aatatcgatt gaacaagttc cattctctca ctggttatac
     2761 ccctagaaac tatgacgatt atcatattct aaagctggct tttttgttgg tacaaacatg
     2821 acaactacaa aaaaaccagg cgaacatatg attgttcgtc tggtttttaa ttattaatga
     2881 cttagcgttt ttactagaaa aactacttcc gctttttccc gccaaaagca tctttaaact
     2941 tatcgaagaa accgtcttct tgctgttctt caactttgat gccgccagct tcagcgaatg
     3001 cacgtaatgc ttctctttgt tgttcattta gatttttcgg tgtgatcagt tttactttga
     3061 catgttggtc gcctgttgca ccaccacgta acttcggagc gccttttcca cgtaaacgga
     3121 agttcgtacc tgtctgtgtt ccagcaggga tcttcaattt cacatctcca tggacagttg
     3181 gtacttggac ttcatccccc aaagctgctt gtacaaaact taaaggcaat tcatagtaaa
     3241 tttcagaacc ttcacgatca aagatatcac tttcttctac acggaagacc acatacaaat
     3301 caccaaatgg tccgccgttt tctccagctt ctccttgacc agctaaacgc atttgttgtc
     3361 cttcttctac accagctggt acattcactt taactgaatg tgctttcttc tcatgtcctg
     3421 agccatgaca ggttggacaa ggttctttga tttctttccc tgtcccatga caaacatcac
     3481 aagtttgacg gctcatcact cgtccaagtg gtgtttggcg ttctacatta atcgtacctg
     3541 caccatgaca tttatgacaa gtagttggtt gtgtgcctgg ttttgcaccg ttcccaccac
     3601 aagtatgaca gatttcttcc cgattatact tgatttcttt ttctacacca aaaatcgctt
     3661 cttcaaaaga caagtcgacc gtatattgca aatcagcacc ttgacgaggc gcatttggat
     3721 cgactgaccg accgcctcct ccaaagaaag agtcgaagat atcttcaaaa ccaccgaagc
     3781 cgccaccaga gaagccgccg aatccaccaa atccgccacc agctccacca gcaccgtaat
     3841 ttggatctgt gcctgcatgc ccgtattggt catatgcagc gcgtttttgc gggtcactta
     3901 aaatctcata tgcttctgat acttctttga atttttcttc cgcatcaggt tctttattga
     3961 tgtcaggatg gtattttttc gaaagcttac gataagcttt ctttatttca tcttctgatg
     4021 ctccttttga gagccctaag acttcataat aatcacgttt tgttgccata ggctttcctc
     4081 ctattggttc tcatcatttt cacgtcgctt gttatcatat catattttgt ttgaaacaaa
     4141 aaccgatact tcaaactatt tttcaaaaga aaaagccaaa gtagcaactt tggccttctc
     4201 tcgaaattca gtgaactgat tatttatcgt cgccatcgac ttcttcaaaa tcagcatcaa
     4261 cgacatcatc tgctccgcct tgagctgctt ctgggttttc ttgagcttgt tgttgtgccg
     4321 cttgttcgta tagtttaaca gtcaagtttt gaacgatttc gttcaatgca tcgcgtttag
     4381 ttttcatttc ttcgatgttg tttgcttcaa cagcggcttt caattcatcg cgagctgttt
     4441 ctgctttttt cacttcttct tcgtccactt tgccttctaa ttctttcaat gttttgtcca
     4501 cagagaatag caatgcatct acatcattac gtaggtcaac ttcctcttta cgagctttat
     4561 ctgcttctgc atttgcttct gcatctttta ccatacgttc gatttcttcg tctgttagac
     4621 ctgaagaaga tttgatcgtg attttttgtt ctttttgtgt acctagatct ttcgcagata
     4681 catttacaat cccgttttta tcaatatcga atgttacttc gatttgtgga ataccacgag
     4741 gagctgctgg aatatctgtc aattggaatc ttcctagagt tttgttgtca gcagccattg
     4801 ggcgttcacc ttgtaataca tggatgtcta ctgctggttg attatctgcc gcagttgaga
     4861 atacttgtga tttacttgtt ggaatcgttg tattgcggtc aatcaattta gtgaacactc
     4921 cacccattgt ttcgatacct aatgacaatg gtgtaacgtc aagtaaaaca acatctttca
     4981 catcaccagt gatcacgcca ccttggatag cagcacccat agcaactact tcatcagggt
     5041 taactgattt gtttggttct tttcctgttt ctttacgtac tgcttctaca actgcaggga
     5101 tacgagtaga tccaccgact aaaataactt cgtcgatttc tgattgtgat aaacctgcat
     5161 cttttagcgc ttgacgtact ggtacttttg tacgttctac taagtcagct gttaattcat
     5221 caaattttgc acgtgtcaat gtcatttcca aatgcaatgg tccagattct cctgctgtga
     5281 taaatggcaa gctgatttgt gtgcttgata cgccagataa atcttttttc gctttttcag
     5341 cagcgtcttt caagcgttgt aaagccattt tgtcttgaga caagtcaatg ctgttttctt
     5401 ttttgaattc tgctaccatg tagtcgatga ttttgttatc aaagtcgtcc ccacctagat
     5461 ggttgtcgcc agctgttgac aatacatcaa agacaccgtc tcctagttca aggatagata
     5521 cgtcaaaagt accgccacca aggtcgaata ctaagatttt ttcgtcgcga tctgttttat
     5581 ctaaaccata ggcaagagca gctgcagttg gttcattgac gatacgttct acttctagac
     5641 cagcgatctt accagcatct tttgttgctt gacgttgtgc atcattaaag taagctggca
     5701 cagtgataac tgctttttcg actttttcac ctaaatagtc ttctgcgaat cctttgatat
     5761 attgaaggat cattgctgag atttcttgtg gtgtatatga tttaccttct acatctactt
     5821 tgtaacctgc ttcaccgata tggcgtttga ttgatgaaat cgtatttggg tttgtcacag
     5881 cttgacgttt tgcaacttcc cctacttgga tttctccatt tttgaatgat acaacagaag
     5941 gtgttgtacg gtttccttct gggttagcga tgatttttgc ttcgtttcct tccaatactg
     6001 ctactgcaga gttagtcgta cctaagtcaa taccgataat tttactcata attaacgtct
     6061 ccttctttaa ttgaaagtgt aagttttata tccgcacaat tattgtgcaa caatgaccat
     6121 ggttggtctt aacacgcgat catgtaattt gtagccttct tgtagtactt ctacaatcgt
     6181 atcggctggt gtatcttcag tcgctggaac agtttgtact gcttgatgaa gattaggatc
     6241 aaaggcttct cctttagcag ggattttttc aattccttct tcttccagtg cgttacgcaa
     6301 actttccaag accatttcca cgcctttttt caaacctgcc ccttgatcat ctgacacttc
     6361 tgtggctaat gcacgttcca ggttgtcaat tgatggaagt aattttttgg ctaaatcttg
     6421 tgaacggtat ttttgtaatt gctcacgctc attttttcct cgatttgcca tatttgcgat
     6481 ttcagcacga gcccgcaagt atttatcttc catttcttct aactcagctt ttaatttcgc
     6541 tgtttcgaat tcttccgctt cagcttctga aatacccgaa tcttctgcag cttcgacatc
     6601 gatttccggt tctggctgag catctgtcat ttctttatct agttcttctt gattttcttt
     6661 cacgatacag tcttcctttc attattcagt cagctgtttg attgctctta ctaagaaaga
     6721 gagaacgatc agctgcaaat agcttcgtta ttcacattac gagtttgaca agtcaagcga
     6781 acggtagtaa tcagcaagtt tagctgctag ttctcttcga taaacatcga ctagaccgaa
     6841 catccgagaa taaggcatac tagtaggccc taacaaggct attgtacctc ttccatgacc
     6901 atctatctcg taacttgctt gaatcatact catattctgc aataaatcat tgcctaattc
     6961 agcaccaatc ttgatatgaa tattgctatc catcggagtg atcatttgtg taagtccgtc
     7021 tgagtctttc ataaaagaaa acatcgattt gaactgatca agatcttgac ggggctcata
     7081 atccaagaga ttcatttgtc cactgacata aaccttatct tcaaaaacat gaccaagaac
     7141 ggtgtcaaat aactgagaca tcccttctgt cgtttggaaa tatttatgaa gaatcatcgg
     7201 aatctccgta cgaagtttat ggtacaccgt catcagagga tctccgacta gacggtcgtt
     7261 tacaatctgt accatttttt ctagttcctg acttcccata tattcgggaa gagtgaacac
     7321 ttggctttct acattcccca tatctgttac gacgatagca ataatctgtc gattattcaa
     7381 gggtacgata cgaaagccag tcagtcggcg gtcttttatt tcaggtccca atgaaaaagt
     7441 cgtatagctt gtcagctcag ataatatctc agcagattgc ttaataatct cattgatttc
     7501 atgaaattct ctgcctagag actgacgaat cagcagcaaa tcatcttctg ctacgcgagc
     7561 aggttttaac aaatgatcca cgtaataacg atagcctaaa gcagatggaa tccgcccaga
     7621 agaagagtga gtcttcaaca gcaatccttc atcttctaat gctttcatat cattgcgaat
     7681 cgttgctgag ctggccttga ctccctcaga catcaatgtt ttggaaccaa cgggcacgcc
     7741 actactcgta tagtgttgga tgatcagacg taaaatgtcg ttttgtcgct ttgttaacat
     7801 cttcctcacc tctcattagc actcatatat tctaagtgct aatccactta taaatatagc
     7861 aagaagccct tctcctgtca agagaaagac tccatttttt agcactctaa acacaagagt
     7921 gctaattcta aataattgat gtaaatccag ttatatcaa
//
LOCUS       DBKOIJ010000123         7867 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000123 DBKOIJ010000000
VERSION     DBKOIJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.125"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(115..1479)
                     /locus_tag="ACV5NJ_002174"
     CDS             complement(115..1479)
                     /locus_tag="ACV5NJ_002174"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHY6486224.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1476..3035)
                     /locus_tag="ACV5NJ_002175"
     CDS             complement(1476..3035)
                     /locus_tag="ACV5NJ_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486225.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3091..5571)
                     /locus_tag="ACV5NJ_002176"
     CDS             complement(3091..5571)
                     /locus_tag="ACV5NJ_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486226.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5555..5890)
                     /locus_tag="ACV5NJ_002177"
     CDS             complement(5555..5890)
                     /locus_tag="ACV5NJ_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486227.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5904..6938)
                     /locus_tag="ACV5NJ_002178"
     CDS             complement(5904..6938)
                     /locus_tag="ACV5NJ_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HHY6486228.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(7028..7708)
                     /locus_tag="ACV5NJ_002179"
     CDS             complement(7028..7708)
                     /locus_tag="ACV5NJ_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HHY6486229.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2254 a         1570 c         1427 g         2616 t
ORIGIN      
        1 ttttggcgaa tattatgctc aaaaaattct tccgctattt cattatatga attttttatt
       61 ctcgattgat taatatttat tatatcccca cacaataaaa gaccgtgata tttctcacag
      121 tcttttatct actctattgc tatttgctct cttagctagt actttaaaag ctaactcatt
      181 gaggtagtga aaacgagagg tgtattcaga cagaaatcca aaatcctcaa ctgctagctg
      241 tggaatcgta gttttgtgtt tcacgatatc cgcaagcagg ttcaaatctg ataataagaa
      301 ttcttggctt atcagctgtc cgccaattat ttctaatgta tccggatggt agatcagctt
      361 tgcatttact gttttgtttt cttcgtcgta ttgtggaaat agatcaaatt ctcctgaaca
      421 cgaaccggta ttttgttcca agaatggcgc ttcatcttct gtcagcccta cagttcctaa
      481 aaagtagtct ataatgactg ctgctgttat cttttgtctg aggtcgtaag cggttggctg
      541 accactcaaa gttagcgcta ctgctcttgc catatgtaca gctcgagtaa ctaaaggtaa
      601 aaagagatgc cgtttatttt tgtcaaaact gactgggacg atatctccaa tcgcatagat
      661 gtccggatca cttgtctgca gatattcatt gactatcaca gtctcatcct catacaaagc
      721 cactttttct tttaacaatg tcgtattagg acgagtttgg gcagaaaaaa tgaccatatc
      781 tgcctttatt gtttgagaca gaagttgtac ggatactact ttctcttctg attttttcca
      841 atcgatcaag aattcatttg gataaaaatt tatttgctta gtttgcagct gattttgaaa
      901 actgttgatc atatccttat cgaaatatct tgataaaaca ttcggcatat aatcaactag
      961 atgaataatg tatcctttat ttctcaaagc atctgctaat tccaatccaa tgtaaccagc
     1021 accgaagatc actacttctt ttgcttcttt taaagcggca agtacttgct tagattgcac
     1081 tagattttta taggtgtaga cattttcagc ttcaatttgt ttatacaagg tgacaggaga
     1141 ggaacctgtc gctacgatta atttatcata ggttatggtc tcgttttttc cttctttgga
     1201 tgcgaggatg agctgatgtt tgtctggatc aagttcagtt actctccatt cggtgatcag
     1261 ttttgcccca ctttcttgaa gagtccgcat gttatgggca acttcagcta aactacgtat
     1321 ctttcctttt aaataaaggt tgattccatt tgaaacatag cctagtccat ctttatgatc
     1381 ttcatcgatc agtaaaactt ctgctttcgg ttgaagtttt cttagattta gggcagctgt
     1441 gatgccttca tgggaagcac caatgatcac gattttcatg ttttattcat ccttaaattc
     1501 aatttttcta aataacattc ttttaaaaat tggttcaaat aggggaaatc ataattatat
     1561 tcgttgctag tgaatacaaa aactttagcg gacttctgtg acgggtactc tttcaattgg
     1621 ctagtcaaaa tgatatcata ctgcttttct ttctcgtatt cgactaaatc atatttgatc
     1681 gtattcagca attgttcctg gatgatttct ttcacgatat ccgccattag cctttcataa
     1741 ggaagatcat aagcaatttg cagacgtaat tctaactgct tgatcagcgt ccgtaaaata
     1801 gatgcatagc gagcaaataa ttcaaagcgg gcattttcct cgtctacttc agtaaaatca
     1861 acaatagcaa atgtttgtct gaccaatctt tcacataaaa ccaggaattg tttatcttct
     1921 acttcagtta accgctgttt taatccttct tggccaaaaa tattcatcca tcctctaaaa
     1981 taggcaaaat gagcgtgatt ttgcatcact gtcaacgcaa tcttttgcga aatctcttct
     2041 ggaaaaacat aaaaagagaa atattcttct atcgattgtt taaacgttcg attcaattga
     2101 gctactacgt cgtctttgta tttttcgctt atcaaaaact cctggacatg cgagttatgt
     2161 gtatctaatg taaagttact gatcatgaaa agataaaaaa ccaacgtttc attttgattc
     2221 caaggaaagg catattgaaa gaagtaacgg gtcaaaaatg tatggaggtc caataaaaat
     2281 tgtgtcgttt gaaacaatcc aacaggaaga ttgctataat ctggtttgga ttttttcgta
     2341 cagcgcattc gcttcttcat aatggaaagc catatttcaa atttcaatcg tccttcttct
     2401 gtaatagcaa aactaaactc tttttgcagc agatctaaaa aagtgtttcc tgataaatca
     2461 ctgtattctt ttttgttttc aagataggga cgtccaaacc aaaataattg gaaataaaaa
     2521 taactaattt gactttctgg accggtcagc cgcccgcttt ttattttcag atgaaactca
     2581 tttaaagaat catttaattc cttcactctg cgagacaaag tagccgaact gatattaagt
     2641 tccatgacaa gtttctgccg atcaaatgag ccattttcca acagataaag aagtatctga
     2701 tattttgtag aatagcgcaa gtaacaatga taataaacat cgactgctgc tatatttcca
     2761 tgtctgatta ataaaatatt cccttcttgg ataacaattt ccaattgttt gatattttcc
     2821 aagttagctt gtaaataaaa cagatcttct ttcaaggtag cccgatcaac tgataagcgg
     2881 gtaagtacgt tattaacgga aacctctcct ttttcctcta ataaaagttg gaaaagtttg
     2941 atttgccgtt gttcattttt ttcaagcaaa ctgaaaagtt ccaagtccac tccttcaagc
     3001 atttctgatt ttaacagcag aagtttcctt ctcatccttg aaaaagagga tctgtgacag
     3061 ttatcacaga tccatacaaa ttctaatcac ttagctggtt ggcccagctg tcagttccca
     3121 tgtgaccatg ccttgatacg ttgcgccagg ttgagcattc gtactgctat ttacctctaa
     3181 gagtaaacca tcggtatcgg gaaagtcaac gagaacctct cctttagctg ttccatcact
     3241 ctcataaatc aacgtgtttt gaccttgctg aatcaattgc tttgatccag aatgattgaa
     3301 gtataatttt gttccgctta attcttgacc ttgatcattc ttaaataaag aagtcaatgt
     3361 acctgtcagt tgccaaggag acgtgcttgg aagccgagta tcccgcacat caaaagaaaa
     3421 atcgcctttt gattcataat aacttgtctt caacggtttg gggagatcgt taccaaatgt
     3481 aaaatcatct ggtacattaa ctaatgttaa ttcttgtttg gggacaagaa ttgttttagc
     3541 agatgtttgg acaactgggt taggaagctt agcaccatct gctaatgtaa tattgctgga
     3601 aagtacagct gcctttgtca gtatgctgac ggattgtaaa gaagtatcta cagtatttaa
     3661 ttgatagttg taagtaagat gaatggtatc tcctattttg ctcaatagat ctgctgggac
     3721 agaaactcct tgttgaatgc cggtatctgc gatagttgta ccatttaatt ttaaggaacc
     3781 attgatataa gtagtatttt caggaagtga gatcgtgatt tttttgtcgg ttatcgcatc
     3841 cccagatttt gttaacgtga atgtcacatt ttcttgaatc gtatctcctg atagagcaga
     3901 caggggatct cctgtctcag gttgtctgct aaggtttttt agctgactat ctgcactcac
     3961 agatggaaaa ttagccactg ttatttgagg aagagtggtg attccagctt gttcagactg
     4021 ccatttgtac gcaaaagacg gatacatgtt agtaagtaca tcgtccacca agcgataatc
     4081 aaaataatat tgccctgaag ccaatgcagg tagtgtagaa gtagctgtat ttacaccccc
     4141 gttatttgat tggatcgttt ggaaaagttg atctgtccat cctgtcgcta atttttgggt
     4201 tcctttcgaa taaagcctgt aatggatcga accctttttt agttcagttg ctgaccaatt
     4261 ggccgtgact tggtacgatg cagaagaaag gagctttccc cactgactat cggctgttaa
     4321 gtttgttggt aaaaccgttc cgtctagctt gtactgctcc aataccttta aagcaacagg
     4381 agaaagcaat ccgccttgcg catctttccc actgtacacg atattggctg tctgattcaa
     4441 tccacttgtg cttgctggag tatgagtcag tattgctcct tgtgtaacgt tttttcctgt
     4501 aaaatagttg actacgcgat caaagcgact aaatggcaac tgatcaaaac gtccttcagt
     4561 cagccccatc gtcaatgtcc gactattggt agagcctgga aggttctggt tatttccatc
     4621 cacgtattct gtggtatcag caaatgtatt atattttcct gttgaatcac ttggtgagat
     4681 gtaactattc cacgtattta tctctgcact tgcagaaaat gcatatgctc ctggattcaa
     4741 gtttccgcca gttaatttag gataaaatct acctgcagct ggagctaaaa tcgaattgaa
     4801 catacgatct tggataatat tattcgaatc ataaaaatca acagaaaaaa cggccttttt
     4861 agacatagaa ggttgtaatt tacccttggt caaaattttg tttggactcg tatcatattt
     4921 atctccgttt tctgtcatct tcgcgacatt agctgtaaca tcaaattgaa gcgggatatc
     4981 gtccacgttg aattcgcctc gcgtaataat agcaataata tctgtcaata gagtatttgt
     5041 atcccacgac cccatcccaa aacgcaaggt atggcttcct gctgataata aacttccgct
     5101 agtaaccttg atgggggcac cggcaatata caagtaggcg ttgttccagt caattgcttt
     5161 taatacatca ctgcttgata catgttccgg caaattgatg atcgccacaa atgcaaatcc
     5221 ttgtgcagtg atattcacca aatcaagtac atttgttagt ttcacatggc tttcccaatg
     5281 aaaacggacc gtgccacctt tccaaggatc atttgaacca tcgttcaaat aagtagcagt
     5341 caatttaggc aattggttag ccccagttag ttggacaagc ttcccgccat tgactgcact
     5401 acgtccacca tttggaggta cgatattttc atacgtgtat ggatcataag aaatggcagg
     5461 aggcgttgct ccaacagctt ctgccgaagg tgaaaagata aatatactta aaacaaaaag
     5521 cgaagtaaaa aagaaggagc gaaaaaatga aaatttattt tgtagcaaca tgttcatcct
     5581 ccttttcctt tcttctccat aacaacaacc acgcaagaag ccataattct ataccaatga
     5641 tcaaataaag aaaacggatc ttttgttcat tcgtctccgg gaatacgcca tgttgattag
     5701 attctttata aatcggtagc tgtgtctgct gttttgatgc gccattgact gaaccacttg
     5761 gatgcggaat tggacttaga tcataaaatg taatcgtacc tgaacttttg gtatctgctt
     5821 ggactggctc ttgccagctg aagaacagca aacttgcaat aatccctccc aataagatcg
     5881 atgttttcaa cgaaatcacc tcatcatgtt gtttctcctt ttttcttttt cttgtggacc
     5941 cacaaaaaca gcaaaatcaa ccataacact agaactgcag caaccaatcc tgcataaagc
     6001 catgggaaag attgttcttg tactgcaaaa acggaatttt catttacttt ttgtgctttt
     6061 tcttttttga tcgtaaagtt ttgtgtaaat cgccattcat tactgtcgcc tgattttgct
     6121 atgatttctg cgcggtaatc gccggctttt gctttatcac cagctaaatc aagattcaag
     6181 tgaaaattcg aatttggtgc aactaaaaat gcattagacg tattttcaat ataaagctga
     6241 ttttcataaa agatcttgct cgtaaatatc aaattgctga taatcctagg ctgaggattt
     6301 tggaaacgaa gctgaacagt tggctttgcg ttgacctcgt ccaaatctcc tgataaaagt
     6361 ttcagatccg gttctatcgt ctcttgactt tcttgtaaaa cgactgcgag attataagca
     6421 atacgatttt tgatttgtgc tccttctgta gcattttctg tttcagcttc ttctttaggt
     6481 gaaacatgaa tacccgctaa tacacgtccc ttaaagcttt ttgctgggat tttcacttct
     6541 ataggtacag ttgtttctgc atgtgctgat acattgattt cctttttcgc aatgagcaaa
     6601 acgtcacgga catcaaacgg caatgtctca tctaacgttg ggtttttttg actataatcg
     6661 atcgttccac catcactcgt taccgccgga ttgacagaga cctcaaatgt tcgttcttca
     6721 tctgaagaat tgctgataat tacttctagt gtctgatttt gttccggtgc cactaataaa
     6781 tcaaaataac cgattgcttt atctatttgg ttctctggaa ataccggttt gattccaaag
     6841 tctcctgctg aatcctttgc ttggctgatt gacggaaaaa tgaaaaatag acttaaaata
     6901 gaaaaaaacg aaacaaacag tcgtttactt tgattcattg tccaccacat cctttcaaca
     6961 acaactgcgg taaaaaatcc ttcacctata gtattttcac cgcagtttgt tgaatttatt
     7021 tcatactcta tgctactggg ccagccacga ggttccaagt caaatcagcc gtgaatgttc
     7081 cagctgctgc tttcggcact ttcagtgtga tatttgctgg atcataaaca gaaagccatg
     7141 ttcccagacc ttgatctttt gctgctgtaa atattggtgt cgctgcactt gctttactca
     7201 acccagttac agtttgacta gttggtccag tggattcgtt atctgacgta cttttaacag
     7261 tggatgttcc aaaatctaat tcagcgcctt gcaaaacttg tgaaccgttt acaaaatctg
     7321 aaaccgaaac tgtcacgttc cacccttgtg cttggccatc tgcacctaca cctcgacgat
     7381 ctgatacttg gagataaggt gtatcgtttt ttgaagcgtc tacttgatac gtttttgttc
     7441 cgctttcaat ctcgtgtgta ccaaatggca attcgggaac tacatccagt gttaaaggac
     7501 ctgtctggcc agttgctgga ttttccggat ctgctggctc tactggtttt gaaggatcag
     7561 tagggttaag aggctccacg gtatttgttg gttcagtaaa ggtaatatct ccttttgaag
     7621 tcgcttgacc ctcagcaaaa gctggtgttg ccaagccgcc tagaagaagt gttgaaagta
     7681 ataatgtccc aatcatgttc tttttcataa taaatcactc ctttttctta gtgacctaag
     7741 tatagtagag acagtatgca aaaaatcctc atgaattcat ccgcttactt gaaattatga
     7801 ccctttttgt ataaaagaga aaaaaacaat aaaatcgttt acatgaatat tcttcctttt
     7861 ttattaa
//
LOCUS       DBKOIJ010000124         7848 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000124 DBKOIJ010000000
VERSION     DBKOIJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.126"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            100..801
                     /locus_tag="ACV5NJ_002180"
     CDS             100..801
                     /locus_tag="ACV5NJ_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HHY6486230.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1410..2426)
                     /gene="tsaD"
                     /locus_tag="ACV5NJ_002181"
     CDS             complement(1410..2426)
                     /gene="tsaD"
                     /locus_tag="ACV5NJ_002181"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HHY6486231.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2423..2887)
                     /gene="rimI"
                     /locus_tag="ACV5NJ_002182"
     CDS             complement(2423..2887)
                     /gene="rimI"
                     /locus_tag="ACV5NJ_002182"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HHY6486232.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2894..3436)
                     /gene="rimI"
                     /locus_tag="ACV5NJ_002183"
     CDS             complement(2894..3436)
                     /gene="rimI"
                     /locus_tag="ACV5NJ_002183"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HHY6486233.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3420..4142)
                     /gene="tsaB"
                     /locus_tag="ACV5NJ_002184"
     CDS             complement(3420..4142)
                     /gene="tsaB"
                     /locus_tag="ACV5NJ_002184"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HHY6486234.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4333..5313)
                     /locus_tag="ACV5NJ_002185"
     CDS             complement(4333..5313)
                     /locus_tag="ACV5NJ_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6486235.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5337..6821)
                     /gene="galT"
                     /locus_tag="ACV5NJ_002186"
     CDS             complement(5337..6821)
                     /gene="galT"
                     /locus_tag="ACV5NJ_002186"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HHY6486236.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6833..7822)
                     /gene="galE"
                     /locus_tag="ACV5NJ_002187"
     CDS             complement(6833..7822)
                     /gene="galE"
                     /locus_tag="ACV5NJ_002187"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HHY6486237.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2197 a         1695 c         1392 g         2564 t
ORIGIN      
        1 gtattaaaac ttgtttctaa tgttggtatg ttaaaacttt acaaattctt taaaaaaagt
       61 gacaataaat atagtgaaca ataaggagag gactttttaa tgcgtgttaa taaaatcgat
      121 tttcactttt caaaactcag atatcaacct gattttgaac atttctcgga tgaagagttc
      181 caacagatca aggaacatac tgttcttcgt tcttataaaa aaggacagat ccttttcgac
      241 gatggtgatc ccagacaacg gttgtatatc ttgaccaaag gactcgttcg tttagaacgt
      301 tatgatgaaa gtgcaactta ttactactat gactatgtga atacaaataa cctttttcct
      361 atgtcaggaa tgtttggaga agagacctac acttatacag ctcaagcgat gactgatatt
      421 gaaacattct accttcctgt ttcctactat gaaaaattag cacgaaaaaa tcccgaacag
      481 ttgatctata tgatacaaaa aatttctaaa gtagttgaaa tacatgaatt gcgtatccaa
      541 atcggattga cctctagcgc ctttgaccgg gtaaaacaaa atttattcat tttgaaagaa
      601 gaattgggcg taccaacaaa agatggtcat atcaccattc cttatccgat caccttaaaa
      661 gaattagcag tgaatagcgg cacgacacgt gaaactgctg gacaagtaat caaacaacta
      721 cgtgattccg ggctgttaga ttatgacaaa aagcgtttta ctttctgtaa gacagctact
      781 actccagcta tgagagaata acgcgaacaa aaataagcag tgaccctgat aaatatcagg
      841 gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta gatcactgga
      901 caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt ttgcaaaaaa
      961 tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt attcgatatt
     1021 cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat gataaataag
     1081 ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata gggctttgac
     1141 aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact cttcggtcac
     1201 aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt gtgaatcaga
     1261 gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg catacgcggt
     1321 aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt tcttattact
     1381 tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct tggatcagca
     1441 ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc tgctccaatc
     1501 attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac ctcagggagt
     1561 ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc tacacctcca
     1621 gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt agtaatcaat
     1681 acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag atcttctcca
     1741 cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct aaaactgaaa
     1801 tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt tccctgatga
     1861 gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct tccaactttg
     1921 tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata tgatccatct
     1981 tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat caatggaaac
     2041 tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt tacaggaata
     2101 agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat cagcaacgag
     2161 ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc cgacacacca
     2221 gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg gcggctagct
     2281 acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc aacgatgtta
     2341 gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca acttgattca
     2401 atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt ctgctcataa
     2461 tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat atacgaaact
     2521 tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct aaaaaaacat
     2581 gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt gcatgccctt
     2641 ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct attactttcg
     2701 agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa tcagagtgag
     2761 gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt ggataagcat
     2821 tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct ttttttctgc
     2881 aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca tcttcttttg
     2941 tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc cgataaagtc
     3001 tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag cgattcatta
     3061 tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc tgatatgccg
     3121 gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa ccgataaaac
     3181 caatcattct tccattatca aagataccta gatatagatg aggaagcggt gacttcaatt
     3241 ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct cgttcgacat
     3301 ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga cgatcgattt
     3361 gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg gcatacgctc
     3421 taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca tttttcttct
     3481 gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac tggttcaact
     3541 aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg aactgtatta
     3601 atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca atcactcccg
     3661 acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga agcagaaata
     3721 tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc cccagcgtat
     3781 acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc gatgcaatta
     3841 gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt ataagcaatc
     3901 gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc atcagaaacc
     3961 gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg gtcgattgca
     4021 ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc aagaatttgt
     4081 tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc taatgtaatc
     4141 atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata cttttttatt
     4201 ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat ctgctggcgg
     4261 gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcactccat tttcccacag
     4321 cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca caataatctt
     4381 tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg ctagctctcc
     4441 catccattcg gtataaactt ttacagtaga aagcgagggc gttacatatt tcgctacact
     4501 gacatcattg aagcccatca cagaaatatc ttctggtacc actaaacctt tttcttgaat
     4561 cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat caggaacatg
     4621 cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag aaaaatctgc
     4681 ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa atgccataat
     4741 tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag caagcatagc
     4801 gatatgttgc gctttatggg agacaaagtg ttcaatgact ttttgaacac ttgacttaaa
     4861 gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca aaagcaaggc
     4921 attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta gtgcgatcgt
     4981 tccatctgcc ggaagatcac taatggaatc ccacgtttct ttcaataaag taacgtttgc
     5041 ttcttctgcc tttttttcga ttcctaggcg aatagataaa taataaagat cttcaagctc
     5101 ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg ctgcattttt
     5161 acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct ttgtggctaa
     5221 gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag gagaaactcc
     5281 tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt ttacgtttat
     5341 aaaatcgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt gaatactcca
     5401 gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac atcggtgacc
     5461 gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg acgatgatat
     5521 tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag ttctggtttc
     5581 aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt ctcctttttg
     5641 atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc tgaaacattg
     5701 ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc aattggcgta
     5761 atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt ttcatccgaa
     5821 tatgctttcc atttttccaa tactgagtta gctgcttcaa taagcaagtt tttctctgtt
     5881 gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac ttgcggataa
     5941 tcttctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc atgacgtcct
     6001 gcctgataat gatcatgaga aagaatcgat cctccaacaa ttggtaagtc agcattcgaa
     6061 ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa tgtttccttt
     6121 gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt gtaataagca
     6181 taaggggaat attggaatcc ccagctttca ccgtctagat tcattcgaat aatccggtga
     6241 ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat gcaaagcatg
     6301 caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt tggatctttt
     6361 tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc tgccggaaat
     6421 ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact taaattaaag
     6481 aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa tgcattgacg
     6541 actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc ttgttctgct
     6601 ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc caataagtct
     6661 acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc tcctaccatc
     6721 cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc agcttcgatc
     6781 gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc ccctattctt
     6841 cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatc gctttgatat
     6901 ctgtgtattc aggtttccag cccagaatct ctctcgcttt ttcacttgaa gcgactaatc
     6961 ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt cctgtcactt
     7021 ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct aaattgaaga
     7081 agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca gcaatcaaat
     7141 cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa tcgtctccat
     7201 aaacagccaa ggcttttctt tgaccaagag ctacttgtaa aatgattggg acaagatgcg
     7261 tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct acattgaagt
     7321 agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc attttttcca
     7381 tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct tctgtgattg
     7441 gagactcttt cggctctcca taagttgctg cagtagaaga gaatacgatt ttattcacgt
     7501 tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg ttgaaataca
     7561 tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg attactcctt
     7621 caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg tctccttcat
     7681 aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta tctaccacag
     7741 ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata taaccggctc
     7801 cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaa
//
LOCUS       DBKOIJ010000125         7844 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000125 DBKOIJ010000000
VERSION     DBKOIJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.127"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(39..2138)
                     /locus_tag="ACV5NJ_002188"
     CDS             complement(39..2138)
                     /locus_tag="ACV5NJ_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HHY6486238.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(2439..3263)
                     /locus_tag="ACV5NJ_002189"
     CDS             complement(2439..3263)
                     /locus_tag="ACV5NJ_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HHY6486239.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(3260..4015)
                     /locus_tag="ACV5NJ_002190"
     CDS             complement(3260..4015)
                     /locus_tag="ACV5NJ_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HHY6486240.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(4028..6004)
                     /locus_tag="ACV5NJ_002191"
     CDS             complement(4028..6004)
                     /locus_tag="ACV5NJ_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HHY6486241.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(6057..6728)
                     /locus_tag="ACV5NJ_002192"
     CDS             complement(6057..6728)
                     /locus_tag="ACV5NJ_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HHY6486242.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(6788..6997)
                     /locus_tag="ACV5NJ_002193"
     CDS             complement(6788..6997)
                     /locus_tag="ACV5NJ_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486243.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(7064..7303)
                     /locus_tag="ACV5NJ_002194"
     CDS             complement(7064..7303)
                     /locus_tag="ACV5NJ_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486244.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            7713..>7844
                     /locus_tag="ACV5NJ_002195"
     CDS             7713..>7844
                     /locus_tag="ACV5NJ_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHY6486245.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         2163 a         1595 c         1197 g         2889 t
ORIGIN      
        1 aaagaaaaca aggctgattc tcagccttgt ttttatattt atttgagttt taaaactaca
       61 tagcccaatg atgctaaagt aagaaataat atacttccaa taacagttag cactaaatga
      121 ttcgatccag tttttggcac aatgatctga ggtaagttag gttttacccc aatcgttact
      181 acattagttg ctttctcaat actttctact gaaacttttg cttggttgtc gatatccaat
      241 tcttcattca aaggagtttt agctgtaaat atcagctgaa tttctcgtcc atctaaaaga
      301 tttttactta agaaatcagc ggaagcttcc cacttcagcg ttcttgaatt gctatcatag
      361 gttagtgtac cttctgaagt aatatctttt cctgtactca tatctatcac tcgcgcagaa
      421 ccttctttat acgtcaaacg atcttccact ggatcttgaa tactaaattg cttgtaatga
      481 tctgaggcaa tgtctacacc ttttgtatgc catttttgtg tgactagata ttgtacttca
      541 gatcctgcat ttaaaggagc tgtaggcgtt ccttttttac catcatttcg accatcaaca
      601 tctttcccat cttgttccac ttttttattt ggtttaagag agaaatagtt ttctactgta
      661 tttgtttttt gtgttccttt gttcggatca ttgttaaacg ttacagtagc cacatttggt
      721 gcaattcctt ttgcatcatt ttttgctctc atatggagct gcaaaatata atccctaccg
      781 taatttgcag cgttagctaa aaagctgtca gaagcagtat aagtgactgt attacctgat
      841 acagttaact ttcctcctgt atcagctttt ttccaagtac cattcgcttc aacataccac
      901 aatgcgccat catagttttt cccatccgat accatatcaa aatttttgct gtcaaaggta
      961 tcagtgattt taaatgattt gtatttgttc attgtgttga ctgttaatgc atcaaacttt
     1021 tggtgaatat gatagtaaac atctgatcct tgtggaatca cttttttgtc aatattgtgt
     1081 gctgcctctt cagtattagc tttgttaaac acttcttttg taggcttcga tggttgtggt
     1141 gtccatagca atgcagtatc aaagctaaac atcatactat tcgtatttgc gccaatcatg
     1201 aatgttgggg aagcgccttg aagcgtatac gttacaccac ttctgtgata agtatcagct
     1261 cctacaacat cggtgaaatg atcattcgag ctataagcct tttctgcacg tcctcccact
     1321 acttttgaag ttaaattgtt tgttgataac gcaggagctt tgtattctgc acatgctgta
     1381 tctgttgtga caaaataagg taagctaccc attttttcat acccaacaaa ttctctcata
     1441 tcaatattac gttcaccgac agattcatta ttgtcattat accaagacac acgttgctga
     1501 tactgtccat ttaaactgta aatggataga aatgtatcgt ttaaatcaat accagctgct
     1561 ctcatcgatc tcatttttga ttgaatttct ggctgtggtt cactcaaagc tacttttgtt
     1621 tttgttcctg catacttaaa ttctagtttt gcattcatga cattgattga ctgcatgaaa
     1681 aatcctcgat aaatattttt atcaaagagc aaataattat aatacttttt gccattaatt
     1741 gttttagcta atccgtcttg gttaacccca gcgtgcgcta caaaattaga aaaagtcacg
     1801 tcacaatcaa tttcttttcc tagataagta cctacttttt gataatgggc aacgattttt
     1861 gacgtacttt tcaaatcttt gacttttggc caagcaagta ctttttgttt tttagttgaa
     1921 tactcatcta gtatcttaaa tccattgatc ccagttactt cgatatttct caatgaatca
     1981 ctatcatgat aaagcaccag attattattt tctttgattt gactcggatc aactttttct
     2041 aaggcctgga ctgcagttcc cctcgcaaaa agaagaagga acaaaaacaa cactaaagta
     2101 attactcttc ccgtagtgca atgaatttta ttttccataa tgccctccat ctaatatttt
     2161 tttaaacgat cgtaagcttt tcattctttt tttcctaata cccacgttac caatcctgta
     2221 agaagaacca aactaaccat aaaaattgga ttgatcaaga gctttttaat cacctctaat
     2281 ggcatggaag cacattgaat gtttagaaaa tgaatgaaca atcgttgata tcttggataa
     2341 attgataatt gagaaaaaat taaaccgcaa cacaatattg ttgcggctaa aaaggataga
     2401 ttaaaaacta atattcttat tattttcatc atttcatcct aattatcaaa aaataattga
     2461 gcaaaaaatt cccaacaatt ttcaaaaaag ttcttcttta caccagttga cacaactttt
     2521 ttaatttctt ctttcggctc tgatttctct gatttagggt ataaccaaac atccgtcaaa
     2581 aattcacctt tttcatttga aacaggtgtc aataaaacaa tatcttcact gtttgaaatg
     2641 ttttcagggc ttgattcatt aacgatatag tatgcttgtt ttttgtttgc aggtacttca
     2701 atggaaaatt caatcacgcc ttcctgatca tcaatcgttt tggtttctcc ttcagccact
     2761 tttttcaact ggtcgtatga aagttttttt gctcgatctt tcaattttga ctcaattatt
     2821 tctacagatt gatttttctc attatcatct tgtgacattt cttctaagat attcgtgata
     2881 tcgtagacaa taaacttcgc gccattgata ccttcttgct gggctacttg actgccttga
     2941 ttgttgattt gattcgaaga aataattttt tgaatatgaa tggcttgctt gatcacttgc
     3001 gtatcttcta cgtcttgcgg cttttctgaa gtctctgaag aactttctga cgttggattc
     3061 gttgattcag taaagcttat atttgaatct gccgtttctt ctgtagtatt ctccgaactg
     3121 tccgtttcgc ttggtgcagt aattgaatta tctattgttt gagtggtact actatcactg
     3181 ctgtcagcga aagacagttg cgaataagct acagtagaaa agagagctgc tacacaaaca
     3241 aacagccatt ttctcttatt cattccgatc tcctccttat ttttttataa ataaaaataa
     3301 taggaacgca aaaaaaagcg aatagcagtg ctaatcgcca atccaatgaa attgatttcc
     3361 tattttttgc ttttttctgt agttttaaat cttctttact gaaggaaact cgttcaccat
     3421 acactaaaaa gcggtgtgag ttgacacctt ttggtgtgca tgtcattaac gtgatcctat
     3481 cttggtcttt ttcaggcata agataatcta aataattttt ttgtaattct tctgctttaa
     3541 cttcttcaat tcgataaatt tgataggcat gttgttcttc atttacctta ataaagaatt
     3601 tttgcccttt ttttactgaa ggcaaatcat cgaaaagatt atctttataa agtccactgt
     3661 gccccgcgat taaaggattt ttaccgtttc ctcctgtaat atcgccagtt ccttccgtaa
     3721 tccctacacc attctctaaa attttgtcgg aagttccgtc ataaattggt agttttaaag
     3781 aaatacttgg aattgtaaga acaccaacag gatcgcctaa ctctattttc gcaacatcca
     3841 aagaagaatc tgttttttca agattttcaa tggctaaaat tctttgctgt tgttgaaatt
     3901 tcgctacatt tgtttccaca actggaatta aatagactgc tactgccgac agcaaccaaa
     3961 aaaatatcat cagtttgtgc atgactcttc tttttggggg cttacgaata atcattagct
     4021 gatccttcta ggctttcttc ttaaagtaaa caactgcaga aagtccccct gctcctagca
     4081 acaagcaaga gatcaatacg taagattgga aaccgttgct accagtcatt ggtacggaag
     4141 ctttttgatt atttttgatc gtgattcggt tttcttcttt gtatgaatct ttgtctactt
     4201 tgaaagagat ctcttttgtt aatttttgat agttttcagg agcttttgtt tcttccagtg
     4261 agtaatctcc ttcagttaaa ccagtgattt caaaatgacc ctctttatct gattttagga
     4321 ttgctgcatt tgctacatcg tctacccaaa ctgcttcttt gacaccatct ttatctgtat
     4381 cagtaagtcc accgtagtac ttaccttctg cattcttagc tgcaaattct gcgccttcaa
     4441 gcggtgttgc tttgatacct gttccaaata ggccgctatc gacttttaag aattttgcgc
     4501 cataagtcac aacgtcagct ttcgtttttt cgtggtgttc ttcctgatta aatggattat
     4561 tgttataaac gaaatccacg ctatttgtta atggatttgc gatttcagca ctatcattca
     4621 caaacatgtt ataagtcatt tttagatctt gaccatgtaa attcgcaact gtatcactta
     4681 cttgtccatt atttacgata aaatcgatca caaaaccact atcagaattt tcgattttat
     4741 agtcagtatt ttcttttaat acgactgaac catcattatt tgttacttta atatctgctt
     4801 tttcagcaac ataagaaagt cctttacctg ctgtatctcc ccatttaaat acactgtaag
     4861 gagaagtttc gcttttcaca ccgtttactg tgataccgtc tgcgccacct gcaatgtctg
     4921 ttggaatatg aatcgttcct tgataattta ctgcgtcacc aatttggaaa gaatgttctt
     4981 gtcctgttcc atttgttact gttttatcga tcactggtgc tttatagttt acgtatgaag
     5041 cttttaagtc acttgtcacg ccatcattcg taatttcaaa ggttaattta ttgtgcgcat
     5101 cgttgcgagc gtctgcgcct aataaatatt gatctgctgt tggttcttga tctgatccca
     5161 ctacaacttc cgaaggaact tccacaaaat agtattttcc taacgtcaaa tcagtcgcag
     5221 tcagctttcc ttgatcatcc gatacaagat cacccaattt tgttcctttt ccaggttcgc
     5281 ccttgtataa ctcaaatttt gcccctttta aaagggtttg tttgctttgg tctgcgccat
     5341 cgtcaccaaa tttcgttaat tcaaaactca acttagaaac aatgttctta ggatacaaat
     5401 gaatattttt taaaaatcct gatcctgttt tatctgtcat tggcgcaatc acaaccattg
     5461 gttttgcttt ttgtgtgact aatccagcag cggatttact ttcataaacc acatatacat
     5521 tccctttgga attgacatag gctggttgat cagtaaaagt gatctcacca ttttcatcta
     5581 cagctgaagt aattttgttg gttgcattct tgacgtactc actgttagat cccttgtctt
     5641 ctacatcttg tacaatctcg tccactttcg cgttggttag atcagaatct tctctcggta
     5701 atacttgatc cgtaatattt cctaacgtaa actcaacatc accataatct gccttgttgt
     5761 atttcgttac acctgtagga tattgatcgt gcgtataacc atcgtttttg atcccgtcaa
     5821 tatcaacatt caattgcgtt ccttgatcgt acattaattt atgaaccgtg atattgacct
     5881 tctctggtgg tgttttttgc gtgacatctt ctgcatgtac ttgatacccc tgaaagcagc
     5941 ctgcaatgat tcctaacatt ccaacaagca ttccagcttt ttttacatac cgatttattg
     6001 ccatttttta ctctcctcat ttcatatttc aacgactcgc gttgattaaa accttgttat
     6061 tgtagctctc cctgtaccac gtatctttct tttccttgtg acgtataaac acaagtaaac
     6121 aacgttaata agggtttctc tcctttcttt ggcaaagaca agaaagagtt ctcttcagga
     6181 acgccatcga ttaatttaaa gtacccaggt acgataaatt tacgcgttac cttgtaacga
     6241 tagacgccat cgttactaac taagtccatc acctccccta atgacaactt ttgaatatca
     6301 gaaaataaaa cacctggcat ttccatgtta tgacctgcta ggacatagtt tcctttgccc
     6361 atttcggcat cttcataaaa ataagtagcc gctccaagac tcaaagtgta ttgatttgcc
     6421 cctttaaaaa ttggcaatgc taaaccaagt gcaggaatct gaatttgtgc aaatacttgt
     6481 ttattgacgc ccatttcgat tgcttctttt tgtatggaca acatttgccc caaagacata
     6541 tcattattga cgttcgttgc atcctcctta taactctttg catgtgcatt gatttgatct
     6601 acgggcatct ttttttctat attttgctta aacacctctc cttcatgttt ttggtagcta
     6661 acatagccaa acacactgca aaagacgatt aaagtgactc ctaaaaaacc aaataaccat
     6721 tttttcatcc atctgcctcc tttcagacaa aacaaaaaaa ctttttctca tttccataga
     6781 aaaagtttta aaaatgaatt ctataagttc gtctcaactg ttcaaagttt ttcattgaac
     6841 ctaacaaaca agctttctct tttcctgtta actgcatatc attgatatat tgcgcgacag
     6901 ataatctttt aaggtgtttt tgcttcatcg tttttagtaa cttttcttcc atttctttcg
     6961 acactttttt tctgttttcg atttcttcat aattcatgag ttttcctacc ttccttgttt
     7021 tttgcaaaaa taaaaaagcc tatgatcaca tacgctctac acttcagtct tgggttatct
     7081 ttttctttcg tttaattact ggccgtgctg gtttcaaatc cttgattcca ttatgggttc
     7141 tccaataatc gaattctttt cgcaatcttt ctttttccat atctgttcct ttagataagg
     7201 ctttgaatac ttctttaggt gaataaccac gagctaacag ctgtacatac cttccttttg
     7261 tcaaagtatc catatccatc gcctctaaaa ctggtcgcgc catctactat tcctcctcgt
     7321 aaaatatcat ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga
     7381 ataacacatt cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat
     7441 gtatggaaag gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt
     7501 tagtctgtta cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac
     7561 tagactttca aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt
     7621 aggcgaggtt gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc
     7681 caaaatttat atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta
     7741 gctctgaaaa actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg
     7801 aaatctctgt tgccaatgac atcgaaggtg cttcattcat acga
//
LOCUS       DBKOIJ010000126         7775 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000126 DBKOIJ010000000
VERSION     DBKOIJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.128"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            150..2477
                     /locus_tag="ACV5NJ_002196"
     CDS             150..2477
                     /locus_tag="ACV5NJ_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HHY6486246.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2474..3388
                     /locus_tag="ACV5NJ_002197"
                     /pseudo
     CDS             2474..3388
                     /locus_tag="ACV5NJ_002197"
                     /inference="COORDINATES: protein motif:HMM:NF027869.6"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3461..3976
                     /locus_tag="ACV5NJ_002198"
     CDS             3461..3976
                     /locus_tag="ACV5NJ_002198"
                     /inference="COORDINATES: protein motif:HMM:NF037639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HHY6486247.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3973..4419
                     /locus_tag="ACV5NJ_002199"
     CDS             3973..4419
                     /locus_tag="ACV5NJ_002199"
                     /inference="COORDINATES: protein motif:HMM:NF027869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HHY6486248.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4488..5384
                     /locus_tag="ACV5NJ_002200"
     CDS             4488..5384
                     /locus_tag="ACV5NJ_002200"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HHY6486249.1"
                     /translation="MQKAGNFSGVDKLAGAQFQIKNSENQFVTGFDENTGVALYDPAE
                     SALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQKETTFIVKNGKLQQTTGI
                     IHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHFIKQDVNTQEKLADAEFSI
                     LRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKLISDSEGKFSIEGLKQGAY
                     CLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQPKGILPSTGGMGIIVFIL
                     VGTALVGGAVIYFKKRHQETEA"
     gene            5590..6762
                     /locus_tag="ACV5NJ_002201"
     CDS             5590..6762
                     /locus_tag="ACV5NJ_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HHY6486250.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6924..7418)
                     /locus_tag="ACV5NJ_002202"
     CDS             complement(6924..7418)
                     /locus_tag="ACV5NJ_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486251.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7415..7609)
                     /locus_tag="ACV5NJ_002203"
     CDS             complement(7415..7609)
                     /locus_tag="ACV5NJ_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486252.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2637 a         1459 c         1539 g         2140 t
ORIGIN      
        1 aatctttgta ttaaaggaca agactaaaat aaccttagaa taaaaagaga aaaaaagccc
       61 caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg tgtaaaggat
      121 tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact caaaataaaa
      181 tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct aaaagaaaat
      241 acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg agcttatcaa
      301 gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg acagaggaga
      361 caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag tctagcaaca
      421 acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa aaagaaatca
      481 cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc cagacattta
      541 gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga tatacagtga
      601 tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg gataaaactg
      661 cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat atccataatc
      721 tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt atctatatgg
      781 gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat aaaagttatt
      841 cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt actttttcaa
      901 tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa aatgggcaga
      961 tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca acggttcagg
     1021 tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca gcaaaaatcg
     1081 gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt atcactccaa
     1141 cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc ctagaaagta
     1201 acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag tatcaagact
     1261 attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga agtgctgcac
     1321 aagacctgac gactaatacg cctactatca acggatatac ctttacagct gtttcagcga
     1381 tagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca aatggaaatg
     1441 taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat tataagacaa
     1501 atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt tctgttgtac
     1561 cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta aacaatgatc
     1621 tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat gaaaagggag
     1681 ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg acaaaaagcg
     1741 gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag acatctgatg
     1801 tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag acatatgatg
     1861 gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact agttcgctta
     1921 ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa tcagcaaaaa
     1981 atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata gacaaagtca
     2041 aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga acttatacca
     2101 tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat gatgcccaaa
     2161 gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc gaatcattgg
     2221 attataccct aacagttaac agccggacag atattggatc acaaatagta acgattagtt
     2281 taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc gctaagttga
     2341 cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg ccctttgtct
     2401 ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt aagaaaaaag
     2461 caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg ctgttctctt
     2521 ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag aatcacctat
     2581 tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta tcagtcaaaa
     2641 cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg gcgttacttt
     2701 cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa gtagtgtgga
     2761 agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg cagaaaatgt
     2821 gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg acaaagatga
     2881 gcaaggtcgt gacgctgttt accgatttac ggaaataaag acttcagatt aagtaaaaga
     2941 aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc ataaattgac
     3001 gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca cattaacaaa
     3061 aacagtagag aataagcaga cagattttgc agatggggat aaagtccctt atctcatcac
     3121 cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag atacagctga
     3181 tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag acaagattca
     3241 tacatttcac acacaaaaaa taaaacatgg ttttgtctta tcagattctg gaaaagatct
     3301 tagttcgttt gctggaaaaa aattaacgat ccgttaccaa atgacattga aagaaaacag
     3361 tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa cagtagaaac
     3421 acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc aaacgaaaca
     3481 aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag cgcaagaaaa
     3541 cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta cttccagtaa
     3601 agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag tggagattaa
     3661 accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg aaaaaggcag
     3721 ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag caaatccgac
     3781 gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg aaaaaaatgg
     3841 aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt tagggttatt
     3901 gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag gaaacaatag
     3961 gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg aactgctatc
     4021 atgctgcttc ctttcgttct cggcctagga actgcagagg tttctgcagc cgtatcacct
     4081 acaccagaaa atgttacggt taatttgcat aaattgaaat tcacttcagc accagaaaat
     4141 caaataaaca acggaacgga attgacattt ccgaatagcg aaccgttgaa cggcgttgaa
     4201 ttcaacgtat atgacatcac tgctacgtac tatccaagta aagacacagc agttccagca
     4261 gacgctacac ccttcgcaag tgtaacgact tctggtgaag gattagctaa cctgactctt
     4321 ccaggtaaat ctgatgggaa agatgctgta tacgtctttg tagaaacacc aaagcccggt
     4381 gtcgaaacgt cgccaaatat cgtgttaagc ttaccctaga ccctgacaaa cctttagaca
     4441 cgattcacct atatgctaaa aatgtcatca aagaaagcaa tatcaaaatc cagaaagctg
     4501 ggaacttttc aggggtagat aagttagcag gggcgcaatt ccagatcaaa aattcagaga
     4561 atcaattcgt gaccggtttt gatgaaaaca ctggtgtcgc tctctatgat cctgctgagt
     4621 cagcgttgac attcacaact gatactgaag gatccatcag tgtcaatgga ttaatagacg
     4681 gcaattatac tttagttgaa acaaaagcac cagatggtta tcaattaacc aatccacaaa
     4741 aagaaactac ttttatagta aaaaacggaa aattgcaaca aacaaccggt atcatccatg
     4801 gcgcagatga agccaatctt gggaatccaa acggtgtagt caatcgggaa aatacaacta
     4861 cggataacac gattactgta gaaaatgtcc aaaactacgg tgatttccac ttcatcaaac
     4921 aagatgtgaa cacgcaagaa aaattagccg atgcagaatt ttctattcta agagcaaaag
     4981 atccaactgt taaattaatg aaaaaaatag caaaccttgc ctcaggagaa tacccatatg
     5041 cttggtcaga tgaagtcaca ggttctgact gggaagaagt gaaattgatt tctgattcag
     5101 aagggaaatt ttcaatcgaa gggctgaaac aaggggcata ctgccttcaa gaaacaaaag
     5161 caccagaagg ctacgcatta cctaagaatg aagctgcgta cactgaattt acagtagatg
     5221 atgatccaac aacttctgat gaaacaacga tcgataacca acctaaagga atcttgcctt
     5281 caactggtgg tatggggatc atcgtattca tcttggtagg tactgcttta gttggaggag
     5341 cagtgatcta tttcaaaaaa cgccatcaag aaacagaagc ttaaactatt ggaaacaaaa
     5401 atcatacgat aaataaacga tagtgattca aatggggaga aaggttgtga caagtggtcg
     5461 gtctcttggt attcgctcaa caaacggcaa aataggagga actattttgc cgtttgcttc
     5521 gctaatactc aggtcaaggc gctgatttca caacctcttc atatacatat tagaaaatag
     5581 gtggacatga tgaataaaag cagaataaga atactggaca tcttcatggc aatcatttta
     5641 gtcgttggga tcgggatctt ttcttatcct tttgtagaag attcactgaa tgattttctt
     5701 gcccagcaaa tgatcatcca ttatcaaaag caagcatcga ataagaattc agaggaaata
     5761 aaaaaacagc aggaaaaaat gacaaagaaa aaccaacagt tggcagagca aaacgtatca
     5821 ccagggatag gcagtttcaa ccaagcagtt gatgcaaaag cattaaaaga tttacctagt
     5881 aatgcctttt ttatggaaca tatgcttgga gtcatcgaaa tcccaaaaat caatgtcagc
     5941 ttacctatat tcgatcagac aacggaaata tttttgcaaa aaggaacgtc attattggaa
     6001 gaaagttctt atccgactgg cgggaaaaac acccatgccg ttctttctgg gcatcgtgga
     6061 ctcccagaag caaaattatt tacagatttg cctaagttga aaaaaggaga ccagtttttc
     6121 atccagatca atggaaagac attggcgtat caagtagaaa aaatccaagt ggtcttacct
     6181 gatgaagtag attctttggg tatccaaaaa ggcagagatc tcgtgacttt gcttacctgt
     6241 actccttata tggtcaatac acatcgactg cttgtgacgg ggcatcgtat tccttatcaa
     6301 gcaaaagagg caaaaaaagc gatccaagga attgatcaat ggaaaaaatg gaaatttttt
     6361 atttggttta tcggtatact acttggatca atcggcttag tttggctact catcgcttat
     6421 ttagattctt tggccatcgc taagcgaaac tatcctttat ctttttatgt aaagaacaca
     6481 aacggacgac caatcgaagg aatggtcttt tctgtaaaaa cgctcaacgg caaacactat
     6541 attactagag aaaaagttcc atttgtgaaa gcttctgatg aatacggact agtgagattt
     6601 tctgatttaa aaggcaggaa ctatcgcttg caacatgagg agctcctgct taaaatccat
     6661 gtaaaacata aacattcgaa acagttttca atgaaattga aaaaaggaag atataaactg
     6721 cgaaaagaaa aagaggtgta ctatttgatt gagaaagaat gaatgaagat gtccaaaaaa
     6781 gcagagggaa cagactaaat gaaaaaagag agaagctgac gaaatggacg tgtaaactgt
     6841 tatttttggt ttactatctg gaacgccatc ttctattttt ttatgtttca gaagaatttt
     6901 attattatag tataactttt gaattagttt ccataacatt tggagcaagg ggttaaacca
     6961 cgagcttgag cttcgcttaa agtggcaggg gtatacgtac catttccaca tttatgtgta
     7021 tggtattttg aaccggtcgg tgtgacatag acagtttgtt cattattttg ctcaggagct
     7081 gcggcttgtg cctgctgaga ggcctgttca gctgctgatt gctgtcgcgc ctgttctgct
     7141 tgttgctgtg cgattgcttc ttgcctcgcc tgttctgctt gttgctgtgc gattgcttct
     7201 tgttgcgctt gttctgcttg ttgtcgtgca atttcttctt gctttttccg ttcttcttct
     7261 gcttcttttg ctttgattgt ggaatcaatt gcagcaagac ggcttgtcaa ctcttgtttg
     7321 ttttcaggta gcgcttggat agctgctgaa gcagttgcat agttttcttt tgtaggattt
     7381 gcttcagctt gtgacaaagc agattccgct tctgtcactt tttttctagt gcagcttttt
     7441 ccgctttttg tttctcttct tttttctttt caatttgttc ttgttctttc gatgaatctg
     7501 ccgtactttc ggagatcgtg gtactgctgc tggaagcctg atctgaactt gtgctttgag
     7561 tagtagacgt agtagcacca cacccactca acaaacttcc tgcgaccaaa aacaatgcaa
     7621 ctaatttttt cgctaaattt cctccttaag taaaaaacaa ccgttttcaa taaatgtcct
     7681 tttcaattgt ttacttactt gttataataa ttgtgttatg aatagtgatt gaggaaactt
     7741 aatctaatcc cgtgttgata cacgggattt tttgc
//
LOCUS       DBKOIJ010000127         7750 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000127 DBKOIJ010000000
VERSION     DBKOIJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.129"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            149..1012
                     /locus_tag="ACV5NJ_002204"
     CDS             149..1012
                     /locus_tag="ACV5NJ_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HHY6486253.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1141..2763)
                     /locus_tag="ACV5NJ_002205"
     CDS             complement(1141..2763)
                     /locus_tag="ACV5NJ_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HHY6486254.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3012..3533
                     /locus_tag="ACV5NJ_002206"
     CDS             3012..3533
                     /locus_tag="ACV5NJ_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HHY6486255.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3719..5449)
                     /gene="spxB"
                     /locus_tag="ACV5NJ_002207"
     CDS             complement(3719..5449)
                     /gene="spxB"
                     /locus_tag="ACV5NJ_002207"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HHY6486256.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5614..6432
                     /locus_tag="ACV5NJ_002208"
     CDS             5614..6432
                     /locus_tag="ACV5NJ_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HHY6486257.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6535..6732)
                     /locus_tag="ACV5NJ_002209"
     CDS             complement(6535..6732)
                     /locus_tag="ACV5NJ_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486258.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6901..7479
                     /locus_tag="ACV5NJ_002210"
     CDS             6901..7479
                     /locus_tag="ACV5NJ_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HHY6486259.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7574..7711
                     /locus_tag="ACV5NJ_002211"
     CDS             7574..7711
                     /locus_tag="ACV5NJ_002211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486260.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2314 a         1393 c         1537 g         2506 t
ORIGIN      
        1 ttcttatttc ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg
       61 aaaataaacc ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt
      121 aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga
      181 tctgccatac catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt
      241 gaatgatcaa gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa
      301 tcagctgaaa aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga
      361 atttttccgt tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg
      421 attaagcggc tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga
      481 agaccctgag atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt
      541 attggaagct gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct
      601 ggaaagtaat tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga
      661 acgaggcggt aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc
      721 tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa
      781 caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca
      841 aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt
      901 ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag
      961 tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta
     1021 actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat
     1081 agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca
     1141 ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata
     1201 tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa
     1261 tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa
     1321 gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac
     1381 aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc
     1441 agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa
     1501 gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa
     1561 aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt
     1621 tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa
     1681 tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa
     1741 attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta cattttctac
     1801 ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct
     1861 agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc
     1921 ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat
     1981 ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc
     2041 caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat
     2101 atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc
     2161 aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg
     2221 ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac
     2281 cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag
     2341 accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc
     2401 tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat
     2461 cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag
     2521 tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg
     2581 tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt
     2641 tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat
     2701 attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat
     2761 caattttttt cctcactttt atcattatta gttttgacaa gtcttataga attatatagg
     2821 ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga
     2881 aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca
     2941 gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag
     3001 aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt
     3061 tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg
     3121 tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca
     3181 gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata
     3241 ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg
     3301 tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa
     3361 gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat
     3421 taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg
     3481 gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat
     3541 caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct
     3601 tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa
     3661 aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc
     3721 acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt
     3781 tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg
     3841 gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct gattttgcag
     3901 ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga
     3961 gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt
     4021 ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa
     4081 taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc
     4141 catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc
     4201 ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt
     4261 tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg
     4321 catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt
     4381 cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg
     4441 ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg
     4501 tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat
     4561 cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt
     4621 cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag
     4681 cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta
     4741 gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc
     4801 cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca
     4861 aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg
     4921 tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag
     4981 ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt
     5041 gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta
     5101 gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta
     5161 gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc
     5221 ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga
     5281 gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata
     5341 gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc
     5401 agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact
     5461 cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa
     5521 atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt
     5581 agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa
     5641 ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc
     5701 gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt
     5761 gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa
     5821 gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt
     5881 gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa
     5941 gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg
     6001 cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat
     6061 aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac
     6121 ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag
     6181 gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg
     6241 ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca
     6301 atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa
     6361 cgtcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg
     6421 gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc
     6481 gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca tacattaata
     6541 atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat
     6601 tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact
     6661 gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt
     6721 tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag
     6781 ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc
     6841 aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc
     6901 atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt
     6961 ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt
     7021 taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat
     7081 catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc
     7141 tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat
     7201 agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat
     7261 atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg
     7321 ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta
     7381 gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg
     7441 atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt
     7501 aagagaaaaa tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca
     7561 aagagggatg cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac
     7621 gatgcttatt tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt
     7681 tttgacatta ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct
     7741 atttaagaaa
//
LOCUS       DBKOIJ010000128         7722 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000128 DBKOIJ010000000
VERSION     DBKOIJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.130"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(56..2758)
                     /locus_tag="ACV5NJ_002212"
     CDS             complement(56..2758)
                     /locus_tag="ACV5NJ_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HHY6486261.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2771..4060)
                     /locus_tag="ACV5NJ_002213"
     CDS             complement(2771..4060)
                     /locus_tag="ACV5NJ_002213"
                     /EC_number="3.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HHY6486262.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4210..5256
                     /locus_tag="ACV5NJ_002214"
     CDS             4210..5256
                     /locus_tag="ACV5NJ_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6486263.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5258..7411
                     /locus_tag="ACV5NJ_002215"
     CDS             5258..7411
                     /locus_tag="ACV5NJ_002215"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HHY6486264.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2173 a         1574 c         1409 g         2566 t
ORIGIN      
        1 tctaaaattt ctccaccatc tgaaaccgca cacacaaatt ttgttccgcc tgcttctatt
       61 ccgccataca ttcgttttcc tcctcgaatc cgattgtccg aatctcataa ggttggatta
      121 ttgggacaac cgcttgcttc gttggttctt caagcagatt caatatgaca gtttttccat
      181 tgtctttagt tatttccaat ttttctaaat gactagacat attgaaacct cttactacta
      241 ctttatcact aaacttgctg cgtttcaaag ctgtaattgc aaacgtttca ctttttatcg
      301 ttaaatattg ttgttttgaa atcaatgttc cttcatgatg tttgatttgt tgagtggaaa
      361 aaggaatctg tgctgcgtat gcatgcaaat aagtagagaa tttttcttct ggtccatgta
      421 gttcaatgct gtaattgaac cgatgctcac ctagacactg tgcttctggt gttggaaaat
      481 atccccaatc gcctagttct cccacactgc gaagtagcgt cactgcgatt tgtccgtctt
      541 ctgttacttc atattcattc aaaccaaagt ttccgactgt tacaccgtac tcttcatcat
      601 gaatattgac aaatgcttgc tgatgttgcg gattcgtggg gttttcccaa cttttagaca
      661 catggtttgg gcgcttcacc acttcaaaaa tactatctgc ttcgtgatgt tccacatgta
      721 actttgtagg aaacaagaca cgtaagcgat gatctttcat ctgattgtcg agagtcgttt
      781 caaagtcgat ttttttacta tcttttctga ttgtcatgat tgtttttatt tgaaggattc
      841 gtttttcttt ggatcgttct gcttttcgat aacgaaattc cacaaccatc tgctgttctt
      901 tttctaaacg ctcgtctgca gaaacaggga tttccatttc ctgtagaata cttatttttg
      961 ctatttccgg tgtatctact aatactttta cttcagagtt ttctttgtta ctggatagga
     1021 ttggctgatc acagaaaggt tgtttaaaga tatactcatt tcctatatcg ccgatatctt
     1081 caaaaatatg cagattattc aactttcgtt tgtttttgcg atcttccatt gtaatggtac
     1141 catttttttc aatcgtcaaa tgtagagggc cgttttcgat gattcgtcca ctctgatgaa
     1201 tcattgtttc cttttcttct gctttcgctt ccccttctac aagcgcgaat gtttcccaag
     1261 aaaatgctgg catgttttct aatggtaaag tcactttgat cattcttgcc atatatggca
     1321 cacggaaacg gtctttcggc aaatcataac caaattggac agtttcttca gataaaatag
     1381 ctggtatagt ggccccactt tttgtttcta catgatactt acgtttaggc aaattttcta
     1441 actcttgata tagttgttca ggtattcctt cagcgaatct ttttctttct aattcgatgg
     1501 ttatttctgc ttcaccagtt ttatccattc ctgcagtatt tacaatgaca aatggaaagc
     1561 tgtcttttgg gaatccggtc gtatcgattg ctgcggtcag ctgttctaag gaatcttgtg
     1621 caagatactt tccaacttca tctgcttttt caaaacgagg tatcatttcc cgatggacag
     1681 aatcgactga acaaccgcaa atactgtcgt gaggatgatt ttgcattaat gtcttccaag
     1741 cataatctaa ttgatcatgg ggatagtttc cactaacttc ataagccatc gtcgcaagtg
     1801 gttctgtaat attttctagc tgtcgctgga ccttggtatt ccattgtttt aagtaaaccc
     1861 gagcagatgc tgtattggct aaagtatacc agccatccgt ttcttgactt gtcaattctc
     1921 cttctactga gcctaagtct tctggcacat ctttttgaac tgcttccaga tagtcagtaa
     1981 aattagaatg aataaactcg taatctggaa acagttcatt tgcgagatgg attgcttttg
     2041 atatatcttt ttgtaccggt tgatgatcaa caccattcat catcaataag tggttcgtag
     2101 aggcatattg ttctgcatcc gctaattttt gcttccaaaa ggcaagagct gcttcttttt
     2161 ctactggaat ctcattcccg ttgctatacc aattggcaaa tagcagacca aagattgctg
     2221 tttgatcagg tcctttccac cacatctctg agtattggga agaatagttt tctgcttcca
     2281 aaacttgatt atcaaagcca attggtttta caccacggcc aaacgctgct gctgaaatac
     2341 cagcttgttt cattaattga ggcgtctgtc ccatatttcc aaatgtatcg ggaaaatagc
     2401 ctaacatgac aggggttccc catttacggc tttcttccat gccaatcagc atatttctca
     2461 cgttcgattc cgagctgata agaaaatcat cttgcaagat ataaaatggt ccaattctca
     2521 attttccttc attgattgct tgttggattg cctgtctttt ttctggcctt acttgaagat
     2581 agtcatctaa aataatcgtt tgcccatcca aatgaaaact gttgaaggac ggatcaactt
     2641 caaatagctc tagtagatcg tctatcaatt caatcagtcg catgtgatgc tgttcgtaag
     2701 ccatgtacca ttcacgatcc cagtggctat gagaaatgat atatactttc tttttcattt
     2761 acgatacctc ctacttttct actctaatat caaagtaatc catgactaat tcgcaaaaca
     2821 tcatatttgc ccaagaaaac cattctctcg tataattttc aggattgttc acatcgaatc
     2881 cttcgtgcat aaggtgtgtt cctgcgtcag ttgctacaag taaattcaat atccgctctt
     2941 tttcagattt gtctgacgtt gtcattcctt ccatagctaa tgcaatcggc caaacgtatt
     3001 tttctggcgt atgggaggat ccaattcctt ttgcgtactt tccttcatag aaatatggat
     3061 tttctttact caacaatgtt cttcttgttt gcagataatc ttcctcttgt tcagaacaat
     3121 atcccaagta aggtgcggcg atcagattgg gcacattact gtcatccatg actgtggcgt
     3181 ttcccattcc atctacttca tatgcataaa tcgtttgccc tttttgatta accgttcgtc
     3241 cgtgattctg aatcccctct tggatctctt tcttcaattt ctgtgtttgc tgtacaattt
     3301 ctttatcatc gagtatcgtt tgaaaaattt cttccacata acctaacacg accaccgcaa
     3361 acatattgga tggtaccaga tatccataac ggcaagtatc atcgctagga cggaaaccag
     3421 accatgtcat tcctgtaaaa gctgtttttg cacctcgccc gtcattgatc agcgtatctt
     3481 cttttcttgt cgtgtctcgt acaaatgtat aaggagagtg ttcatgatct tgctctgttt
     3541 tgaacacgtc caagattttt ttgatacctt caataaaaga ttgattgaat tgatcagttt
     3601 ttcctgtatt tttgtaaaga agataggcca actgtattgg gtagcataaa gaatcaattt
     3661 catattttct ttcccagatc caatcgttca tttttgtatg atctgtttgg tggccagcac
     3721 cgttagcttc ttcgttgaag gcattcgcat atggatcgat attgatataa aaaaattgct
     3781 ttttgactaa cccgctgatc atgtcagcta attcctcatc ttcttttgca atcactaaat
     3841 aaggacgaac ttgtgcagta gagtctctta accacatcgc tggaatgtct cccgtcaata
     3901 aaaaggtggt tccgtcattt tgctttttga ctgttgtcaa cagagtattg gcaaatgctg
     3961 ctgtaaaatt ctttgcccag tccgcatgga tatccccaca tttttgtgtg atatcttcca
     4021 taaatttctg caccgatttt ggtatatctt tataagccac taataaaacc ttctttctat
     4081 ttgatatatc tttaaagatt atacttcata cagccaatta tgtctacgct ttcaaaaaat
     4141 atctttttat ttactttttc atccattatg gtattatttg aatatgatat atcattaaga
     4201 ggtgtttata tgaatcaacc attgtaccag acaatattta atgatttgca aacggctatt
     4261 ttaactggtg aattacctgt agacagccaa ctaccaactg aaaaagaact ttcacgtacc
     4321 tataaagtaa gcagaatcac ttccaaacgt gcattgactg aattagagca attaggactt
     4381 atttatcgcg ttcgcgggaa aggcagtttc gttaaatccg cttctccgga acagattctt
     4441 gcttctccta caaaaacacg acggattctg tttcttcttc catatgtcgc tgatttgtct
     4501 gttggtgatt ttagcaaggg attgaaccct gtgatgcaag ataaacagat tgatgtcatg
     4561 atgacgacgc ttgactttct acaaaataaa acagcaaaag agctaatcaa tgaatttgac
     4621 ggattgattt attatgctct tgatacagaa caacatcttg acttactttt tgagctttca
     4681 ttgaaagaat tccctgtcgt catattagat aaaaaaatct atgagttacc ttttcctacc
     4741 gttctttccg ataatttcca aggcggtgcg cttgctgcct cttatttgat cgaacaggga
     4801 cattcaaaaa tcgcttattt gtttggtacg cagactcatc cacaatccgt tcgacagcgt
     4861 taccttggtt accttgaagc attggacaaa gctggtttga ctttccacac ttcactggat
     4921 gacaaaagcg cgacattaga tgaattgctt tcttatatca ctaagtatga agtaactgct
     4981 tttgtttgtg aaaacgatgt tgtcgctatc cagaccatga atctcttgag acagcatggt
     5041 tatcatatcc ctgaagattt ttcaattatc ggatttgatg atatccaagc agcagctctt
     5101 gttgacccgc cactgacaac catcgcacaa gattttgaac agttaggtaa gcttgcggga
     5161 gatgctttgg tcacttggtt agagacaaaa gatcgaccat ccgatagtaa atttccagtt
     5221 tcattcatca aaagacaatc aacaaaggag aaataaaatg aatatcggtc aaatagatac
     5281 tagacatgca acagcgaatc agccaaattt ctctaatggg aactgccagc cttatactgg
     5341 cgttcctttt gggatgaatt attttgcacc gcaaactacg gatcaaaacg gcagctggtg
     5401 gtttcatcct gatgaccgcg tttttcaagg ctaccgtctt acccatcaac caagtccttg
     5461 gatgggtgat ttcagtcata tgctgtttac gccgatcaat ggaaaactac aggagaacac
     5521 ccttttccat gctcaaagtt cttaccgccc agaagaaagt atattttgcc cgactcatct
     5581 gtcgatctgt caattacgtt atggcatctg ttctacattg atacctagca tgtatggcgg
     5641 aatcttatct attgattaca gcaaaaatga cagcggttta cttttatcct ttccgggacg
     5701 ccatcagctc tttgttatag atccttacac agtggaagga aaaatcacac attttgctgg
     5761 ttcagaagat cctgattttt ctttttattt tatattgaga tttgaatatc ctttaacaat
     5821 tgatgagtta gctgtttcca acaaaaatag cgactcgatc ccgttgtatt ttgaacagac
     5881 aaaaaaacaa actatccgat ttgggacatc ttttatcagc tcggaacaag cagcttataa
     5941 cctggaacta gaacaaaacc aaagcaaaga gacttacctt gctcacagtc aagaacaatg
     6001 gcagtcctat tttgatcgta tacagctcac acaccatgat caacaacaat tgcggacgtt
     6061 ttatcataat ttatatcggg tgtttttatt tccacaaaca ttttatgaaa ttgatcctag
     6121 tgggcaaaaa atccactatg atacgactag tcgatctata aaacctggtg ttctctatac
     6181 aaataatggg ttttgggata cgtataaaac ggtttatcct ctttattctt tgattgctgt
     6241 agaaaaatac gaagaaatgc tggaaggttt tttgaacagc tatagagaaa gtggctattt
     6301 gccaaaatgg ctgtctcctg atgaacgtgg acttatgcct gggacattga tcgatgcggt
     6361 cattgcagat gctgctgtaa aagatatccg acgagacttg atgcctgaat ttctaacggc
     6421 aatggaaaaa ggtgctacgg ttcaaagcaa acatccaaat tatggacgcc aaggaacaga
     6481 agattacttg acatatggct atgttcctaa tcactaccac gaatcagtca atcacacatt
     6541 agactatgct tatagcgatt tttgtattag ccaagttgcc aaggtactag aagaagagga
     6601 aatgacgaag aaatatcagc agcaatcatt gaactatcaa aatatctttg actatgagtc
     6661 aggatttatg cgttcaaaag ataaagatgg caagttcaga gaaccatttt cagctaatcg
     6721 ttggggacaa gattatgcag aaggaagtgc ttggcaaagc agctttgctg tttatcacga
     6781 tttttcagga ctgatcggaa aatatggtgg cagtgagaga tataaagaaa aactcattga
     6841 gctgtgcaat cagcccccaa cgtttgaccc aggaggatat ggttttgaga tccacgaaat
     6901 gagtgagatg gccgcgatag aatttggaca attagctatt tcaaaccagc caagttttca
     6961 ctatccttac ttgttcagtt atataggaca acctgaaatg gcacagccac tgcttaaaca
     7021 gttgatgact cagaccttcg acgattctcc aatgggttac cctggagatg aggacaatgg
     7081 aagtatggcc gcttggtata tcttcaatag tctgggattt tatcctgtca cgcctggaac
     7141 cggtgaatat gttatcggta tgccattaat tgacaaggct gttttgcttt tgtcaaatgg
     7201 aaaggaattg acgattgaag caacgcctaa tcggccgcag caacaattta tccataaaat
     7261 caaacggatg gacaaagaat atacgaaatt atattttacc catcaagatt tgctgaaagg
     7321 cggcacgatc tcttaccaat tagggatcat ccctcaccct aagaaatatc aagcaaaaga
     7381 tttgcctttt tccttatcaa actataatta acaagaggtt gtgaaaaagc tgtcctgcat
     7441 caagtaataa gaacagccaa gcaaaaatag atcctcatat ttttcgtttg gttggtctta
     7501 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagag gttgtgaaat cagcgttctg
     7561 acctgagtat tagagagaca aatggcaaaa tagttcctca tattttgcca ttttttgagc
     7621 taataccgag ggtcagctga ttgaacaccg tttagtggag aggtcgtgaa aaagctgttt
     7681 agtttcgagt attaagaaaa attttgtgaa aatggctttc ca
//
LOCUS       DBKOIJ010000129         7537 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000129 DBKOIJ010000000
VERSION     DBKOIJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.131"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            107..1248
                     /locus_tag="ACV5NJ_002216"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACV5NJ_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHY6486265.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACV5NJ_002217"
     CDS             1483..1935
                     /locus_tag="ACV5NJ_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486266.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACV5NJ_002218"
     CDS             1949..2638
                     /locus_tag="ACV5NJ_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486267.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACV5NJ_002219"
     CDS             2763..5114
                     /locus_tag="ACV5NJ_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HHY6486268.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5203..5589
                     /locus_tag="ACV5NJ_002220"
     CDS             5203..5589
                     /locus_tag="ACV5NJ_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486269.1"
                     /translation="MEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYF
                     ANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEY
                     NYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACV5NJ_002221"
     CDS             5914..6531
                     /locus_tag="ACV5NJ_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6486270.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACV5NJ_002222"
     CDS             6572..6871
                     /locus_tag="ACV5NJ_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HHY6486271.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACV5NJ_002223"
     CDS             6905..7072
                     /locus_tag="ACV5NJ_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HHY6486272.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACV5NJ_002224"
     CDS             complement(7117..>7537)
                     /locus_tag="ACV5NJ_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6486273.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DBKOIJ010000130         7532 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000130 DBKOIJ010000000
VERSION     DBKOIJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.132"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..636)
                     /locus_tag="ACV5NJ_002225"
     CDS             complement(<1..636)
                     /locus_tag="ACV5NJ_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6486274.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMISTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLT"
     gene            complement(756..1403)
                     /locus_tag="ACV5NJ_002226"
     CDS             complement(756..1403)
                     /locus_tag="ACV5NJ_002226"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HHY6486275.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1487..2914)
                     /gene="uxaC"
                     /locus_tag="ACV5NJ_002227"
     CDS             complement(1487..2914)
                     /gene="uxaC"
                     /locus_tag="ACV5NJ_002227"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HHY6486276.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(2988..4067)
                     /gene="uxuA"
                     /locus_tag="ACV5NJ_002228"
     CDS             complement(2988..4067)
                     /gene="uxuA"
                     /locus_tag="ACV5NJ_002228"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HHY6486277.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4070..5701)
                     /locus_tag="ACV5NJ_002229"
     CDS             complement(4070..5701)
                     /locus_tag="ACV5NJ_002229"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HHY6486278.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6005..6676
                     /locus_tag="ACV5NJ_002230"
     CDS             6005..6676
                     /locus_tag="ACV5NJ_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6486279.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(6836..7027)
                     /locus_tag="ACV5NJ_002231"
                     /pseudo
     CDS             complement(6836..7027)
                     /locus_tag="ACV5NJ_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
BASE COUNT         2435 a         1478 c         1017 g         2602 t
ORIGIN      
        1 agttagttta taacatatta aataacaaat aagtgcacat atcgaaaata ttcccgcaac
       61 aattgtaaaa gttgatccac cacgaactat ttgagcgcct gaatcatctc ttgtataaat
      121 aaataatggt gttaatacac caatgaccaa acttgctaaa gtagctccaa catttcggaa
      181 tgtcgatatc attgttcttt cttctgtaat agaagtaata gctgatgcca ttgatccata
      241 aggaatattt atagatgtgt aaaaaatact cccccataat atatacgtaa catacatgta
      301 tactacctta acgctcatag aagctgaaat catagaagtt tgatacatta aaaaacttgc
      361 taaagcgact ggaccactca ttcttaaaat ccacggttta aacttaccct gcttagttgc
      421 tttcgatcta tcaacaatac gccccatagt tacatcagta actgcatcga taaatcttgc
      481 tactaaaaat aatgttccaa cggcagctcc gcttatcccc aatacttttg tataaaagat
      541 cattaaaaaa gaactagcaa aaatgaaagt aaaatcgttt ccaaaatctc caaacatata
      601 tcctattttg tctcttatac caaattttct ttccatacaa ttctctccct tctgttgtta
      661 agcgttttca ttctgatgaa agtacgtact atactctgaa gcgtaaagag tcagtattta
      721 ttttttacat ttcagagtat tatttgtttt ttctcctaat aattctttat ctcataaaac
      781 ttatctatat atttactagc tactcgactt acttcttcaa aatcaccttt ttcaacagaa
      841 gccaataaat tcccacctac accaactgca acaactccta cttcaaacca atcttctaag
      901 ttctctatac ttactccgcc agtaggcatt atatttacgt gtggtaatgg agcttgatag
      961 gcttttacaa tggatggacc aaaagcagaa cctgggaata gtttaatgat atctacgcca
     1021 gcagtaagtg cctcctttat ctcagtaatt gtcatacaac ctggtaaata aggaatttga
     1081 tataaattac aaatctcagc tgtttgtcga tcaaaagatg gactaacgat atattctgcc
     1141 ccagccataa ttgcgatacg agcagtaaac gcatctaaaa ccgtaccggc tccgattatt
     1201 acatcttttt gatcagcata ttttttagca atttctttga ttatttcttc agcattagga
     1261 actgtaaaag ttagttcaat ccctttaatt ccacccgcaa taattgaatt tattgcttta
     1321 atggcctcat ccttattttt cccacgtaca accgcaatga ctccagccgc ttctaactta
     1381 cttaaaattt ctactttttt catatctatc tccttccttt atggatacat actttgtcta
     1441 gctgtactaa cttacaaaga ataaatttaa gcttttattt caagtattat tttctacttc
     1501 cgatttcaaa aaaattaaaa taattatacg cattacgata acaaatattc tccactatct
     1561 gactaagata catttcatca tctggaattc ggcctgcttt aacccactcg cttagtaatt
     1621 gacataatac tcgacgaaaa tactcatggc gaggatagct tagaaaactt cgagaatcag
     1681 tcaacatacc tgtaaaattc gacaacaaac tttgttgtgc catcgtagtc aactgttgac
     1741 gcattccttc agctgtatca ttgaaccacc aagcacatcc caattggatt ttttgaatca
     1801 tacccccttg gaagctttgc attgctgttg ctagtggaag ccaatcagtt ggatttaatg
     1861 aatataaaat tgtttttggt aagcacccac tcttttcagc ggtatcgaac aatgctacaa
     1921 cattttgtgt caaactagct tgatccccca tcgaatcacc accgacatct actccaattt
     1981 gttcctttaa tatacttgaa ttatttctta aagcatttaa atgcatttgc atcgtccaac
     2041 catatcttgt atactcagac atcaaatgta attgaatacc tgtaacaaat tgtgcaattt
     2101 ctgataaatg gaaatccgtt tttcctgcta aagcttttat taacagattg tcgagtattt
     2161 tttcatctgc tatttcataa aaataagtat tcaaaccgtg atctgctaat cgacagccaa
     2221 tttgatgaaa atgatccact cgttggctca atgcttgctt caacccatta aagtctttga
     2281 ttggtatagc cgctgtttga ctaagtttta aaatatagtt cgaaaagcct gatgatttga
     2341 tactccagac cttatctgga cggaaagtag gtaatacctt gaaagaatta tcctgtttag
     2401 aaagctcagt atgataccac aagtcatctg taggatcatc cgttgtacag atggctttca
     2461 catccatcat ctccaacaat tctttcgggc gataggaatc cgtttgaatt ttttcgttag
     2521 cacgtcgcca aatttctggt gcattcttct cagaaattgt caaatcaata tcaaagtaac
     2581 gttgtaattc caaatgagac cattcaaaaa taggattacc tggcgccttc tccactgttt
     2641 ttacaaaagc taaaaatttt tcataatcat cacctggacc actgatcaac tcttcttcta
     2701 taccattagc tcgcatcaat cgccatttat aatgatctcc agctccttta tcatttaacc
     2761 atacctgagt caaattcttg tattgcttat tttctagcaa atatgctggg tctaaatgac
     2821 aatgatagtc gataattggt tggtgttttg caaatttatg aaaaagtctt ttagctattt
     2881 cattctctaa taaaaagttc ttatctaaaa acatatttaa acactccttt ccttccgtta
     2941 gtttttagaa gagaaaattt ttcctctaaa taaactccct tatataatta tttactcgcc
     3001 cccttttcaa gcgcttccca aataccattt aaataaactg cacctagagc tcggtcatat
     3061 aatccataac caggtctacc ggtttctccc caaatcatcc gaccatgatc tggacgaata
     3121 tagccattaa atccattatc gtacattgtt tttaatatct tatacatatc tagagaacca
     3181 tattctgatt tatgcggcgc ttcgtaaaaa tcgagtccat tgtcacttaa atatttgata
     3241 ttacgaacat gtgcaaaagg aatccgatcc cgttgacaaa attcagatag aatttcatag
     3301 acatcattat ctggtcgttc tgcaatactt ccagtacata atgtaatacc attagaagga
     3361 gaatccacta cattacacaa ccaatctaaa tcgtcacgat ttttaacaat acgaggtaat
     3421 ccgaaaatgg aatatggtgg atcatctggg tgaactgcca ttttaatccc aacttcttca
     3481 catgtcggga taattgcttt gagaaaatat gcaaagttct ctcgtaattt ctcttcatca
     3541 acttctttat aggcttctaa taattgctta atttgtgata accgatgagg ttcccaaccc
     3601 ggcaattcaa aattattagt agaattttct acttctctaa taatttcctc cggatcagta
     3661 gggatatttt ttgaaatata ggctaacgta gaagacccat catctaaacg ataatccaaa
     3721 tccgatttta cccaatcaaa tactggcata aaattataac agataacctc aatcccaaat
     3781 tctgccaaat tgcgaatcgt ctgtttataa ttctcaatgt attgatctcg tgttggtaaa
     3841 ccaattttga tatcatcatg aacattaaca ctctcaataa ctttcaaggt aagtccattg
     3901 gcttcgactt catcttttaa agatttaatt cgttctctcg gccaaacctc tccggctggt
     3961 atatccatta atgttccaac aattcctttc attgctggga tctgacgaat ttcttgtagt
     4021 gagatttttt cttcatttgc cccataccat ctaaaagtca tttccatagt tactttcctc
     4081 caacttctgt tatatattta ttgattgttt tacgaactgc ttgaggacct actagcattt
     4141 cgcaaaagat tttttcagtt ttctttccta ccccaacttg atataaatct aaaccaaaga
     4201 tttctttatt ttctaaaatt tcctgtaccg cacaatgaat ctttgaagga tcttgttcac
     4261 ctaatttcaa tactgataat ttatcctgca actctaccaa taacggatca ggacttggca
     4321 caaaattttc cccttgatca tcgactgcca gtaaataacg taaccaagca gcaatagcga
     4381 taggaataaa tgttagattt tctgcatttc cgtctttttc gacatacttt ttaattgttt
     4441 caccatagcg aatcgctact ttttgagaag tatctgttgc aattcgttgt ggtgtatctg
     4501 gaatataggg attaggcaaa cgtttattaa ttacttcatc aataaattgt tttggatcaa
     4561 taatgcctgg attttcaact actggtaaac cttctaaata acctattcct ttgattaaac
     4621 caaccaaatc atcatcctgc atctctgctg aaattgattg atatcctagc agacaacctg
     4681 ttactgccaa agcagtatga agtggattca agcttgtcgt aaccttcatg atgtctgctt
     4741 tatcaaccgt ctctctatct gtcataatca caccagcctt ttccaattta ggccggccat
     4801 ttggaaaact atcttcaata actaaataat gaactacctc agtattagtg aaagcagcaa
     4861 tattggtatg tttttttgtg tgaatgatat ctaaatcctc gagccccatc atcaatagat
     4921 cttcctttac attctctgaa ggatttggcg taattcgatc aatcatactc caaggaaaag
     4981 tcactttttc tttatccttt aaataagcaa taaatttttc agaaacatat ccttcttttt
     5041 gccaagcttc tgcaatcgta aaaatactat cttgaaaacg ttgaccattc tgtgagaaat
     5101 tatcagtaga aaccatcgca atcggcaact ctccagcatt aaatctgcta tataacaagg
     5161 atgttacaat actcatcgta tgttgaggat tgctaggtcc agatttgata tcagctttga
     5221 ctaccgaaaa gaattgccct ttagaatctt ttaaactgta acctttttca gtaatagtaa
     5281 acgttgtaaa ctgtaattta ggatcttcaa aatatttaac cactctttga tagtccgcta
     5341 tattcttcgg attgcaatat aaactatcag ccgttactgc caaaagtctt tttttgagtt
     5401 caccatctcc actcataaca atttccaaaa tgtcattgtt atagtcatga taagcttttt
     5461 ccactacttc ttcatcaaat gtttcacaca caactactcc tgtttctaat tcattttgat
     5521 ctgctaagca ttgagcaatt tctgcatgaa atcctcgata aagatttcct ccaccaaaat
     5581 gaagccatac tggatgttgc tggccttctt gcttaacttc tttgacatca aaacacggca
     5641 gtagaatctg catttcattg aattcttcga tagaattttg ccaattttta atcgaaacca
     5701 taattaacct cctcaaactg atatgttagt tttgatacat tttatatttt aaaacccatt
     5761 gtaaccgttg tcaatataaa aagggaataa acttgctttt ttctaccata tcagaatgat
     5821 tgtatttttt attcagaaaa ataaaatact ccttcttttt ggactgattt tatcgaaact
     5881 tattttttct ttttttcatg tattctttta tttttataac tagtgtatta cttcaaaatt
     5941 tgatattata tggcatgaac agaattaaaa tatcagttaa aataaaagtt aaggagcatt
     6001 gtgtatgaca attaaaacta gtctccaaaa tcaagcttac caatctattc gccgacaaat
     6061 tatctatgct gacttagaac ctggaaaaaa agtatcagaa aaaaaattag aagaaacact
     6121 ttctattggt cgtacaccaa tacgtgaagc actgattcaa ctccgacaac aaggtcttgt
     6181 tgatacaatt cctcaaagcg gaaattatat ctcattaatt gatttagatt cggccaaaaa
     6241 tgctcgattt gtcagagaac atttggaacg acaaattatg ttagagtgtt gtgccaaaat
     6301 gaataaccaa aaaaaacgga ttttagaaac tataatttca gaacaagaaa aagctgttag
     6361 tcaaaaaaat gctcgtagtt tttttcataa tgataattta ttccataaag cttgttttga
     6421 aattgcagag cgagaagaag tatggcaatg gttagaaaat aacaatacac acttagaacg
     6481 atttcgatgg ctacgagtaa aaacggaagg attaaagtgg gaaaaaatta tggaacaaca
     6541 ttatcaacta tttgaggcat taacaaaaca agatccagaa gaagctaact ttctaacatc
     6601 gttgcatctt catttaatgc ttaatgagca aaatattgtt ttagctactc atccaaaata
     6661 tttcaaaaat atataacttt taactctttt taaattaaat ggttgttctg atcagaaaaa
     6721 tatcgcttgt tttcttatta tgtaatatat ttcataaact attctgcata acaactattg
     6781 tttgtacttt ttacattgga taactaatta tgactgctga taacttttga tattaaacta
     6841 gtatctccaa tacaaataaa aaatagatat cttgttcatc aaaattagtt cgtggatact
     6901 taccttcatt tacatcattc agcggtttcc cattttcttt tattctatta tttcattttc
     6961 taatagactt ggcttcattt cgcaatactt tttttcaata ggcatcaaat tggtgttgtt
     7021 tagatattta gttgtagttt tgtgacatga taagtatgct cctttttctc ttttccacta
     7081 atacaacgat caaagggtgc ttttttcaaa aaactagaaa aatcaaaata agattcgtat
     7141 tttgattcta tatatcctgt ttcaactaca tcttatattt cacaatagaa taaatagcct
     7201 tttgcatagc tgcttaactt ataagaaatt gcaatttact tcttcaaagt gaaaacgaat
     7261 atgagtaaaa taaaattatt cttcttacag ataattaaaa atagcgcgta acaaaaatcg
     7321 gcattcaatc acataattag gattgatgcc gattacttat attctacgta tagttttagt
     7381 attcttattt tttatcacca gtaaccaatt gagaacagcc ctaaagcaga atagtctttt
     7441 atctcaaaac gcttaatttc aggcttagga atatgaataa tcttttgagt atattctttc
     7501 tcttttaatc ttactgaaga aataccactg cc
//
LOCUS       DBKOIJ010000131         7460 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000131 DBKOIJ010000000
VERSION     DBKOIJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.133"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..287)
                     /locus_tag="ACV5NJ_002232"
     CDS             complement(<1..287)
                     /locus_tag="ACV5NJ_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486280.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNK"
     gene            complement(405..665)
                     /locus_tag="ACV5NJ_002233"
     CDS             complement(405..665)
                     /locus_tag="ACV5NJ_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486281.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(802..1215)
                     /locus_tag="ACV5NJ_002234"
     CDS             complement(802..1215)
                     /locus_tag="ACV5NJ_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HHY6486282.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1671..2156)
                     /locus_tag="ACV5NJ_002235"
     CDS             complement(1671..2156)
                     /locus_tag="ACV5NJ_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486283.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2295..2510)
                     /locus_tag="ACV5NJ_002236"
     CDS             complement(2295..2510)
                     /locus_tag="ACV5NJ_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HHY6486284.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2734..3534
                     /locus_tag="ACV5NJ_002237"
     CDS             2734..3534
                     /locus_tag="ACV5NJ_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHY6486285.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3553..5421
                     /locus_tag="ACV5NJ_002238"
     CDS             3553..5421
                     /locus_tag="ACV5NJ_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HHY6486286.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5414..5905
                     /locus_tag="ACV5NJ_002239"
     CDS             5414..5905
                     /locus_tag="ACV5NJ_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HHY6486287.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5893..6447
                     /gene="srlA"
                     /locus_tag="ACV5NJ_002240"
     CDS             5893..6447
                     /gene="srlA"
                     /locus_tag="ACV5NJ_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HHY6486288.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6465..7460
                     /gene="srlE"
                     /locus_tag="ACV5NJ_002241"
     CDS             6465..7460
                     /gene="srlE"
                     /locus_tag="ACV5NJ_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015795 - sorbitol transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HHY6486289.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2651 a         1198 c         1423 g         2188 t
ORIGIN      
        1 tttttattca aaaaaaatag cacaatggca aagatcgtaa gagagatcat aagccccaat
       61 gaatgactaa aaagaaaagc agaatcacat gccatatcat cccatatttc ttcaagaaaa
      121 taaataagcg ggaaaaaggg aaggaatagt agaacatcga tccagcacca tttttttctt
      181 cttttgaaat aacataaaac aataaacatg aacgtgcagg gaagtaaaaa caaagcttta
      241 ctcataacta acaaaatacc agctcctttt atttttttct cccccacatt caaagagtaa
      301 caatggtatg tacttaataa tagaaaaatg gatcaacttg tatgaataac agctgaaaag
      361 atctgcacca aataaaaaac ttccagccaa aactgactgg aagtttattt ttttctgaaa
      421 tattctaaca caaatatgaa aaagatggcc gaagctaata agccgatttc gtagcaaaac
      481 aatgagatac cagagtcgta ataataaaat cctctaatct tatggataaa atagatcatc
      541 ggacaagcag ataagagtaa gtatttgtta ggccttttga ataagtaaaa gaaagcactg
      601 gtcattatga taaatggtat ccaaaacaaa agtttactta agacaatcaa atgatcaact
      661 cccatatttt ttaccaagag agatcataac atctttatcg taaaaacaaa atattttttt
      721 gctcattttg aaaatttaaa catactcgtt cttatccaaa tatataaaaa tcgcccctca
      781 ttcatcgaga ggcgataagt tctaatttaa ctcaattttt tttgtttctt ttggctgata
      841 agctgtttta ggtaatacca atttcagcac accgttttta aaggttccag tgattttttc
      901 ttcatcaaca ttaggaagat agaatgaacg tttgtaagag accgaatgtc tttctttccg
      961 tacatagttg ccatcttctt tatcttctgt atgggactca tgattggcgg agatcgtcaa
     1021 agtattatct gaatattcaa ctgtcgtgtc ttctttgtca cagccaggca tatcagcagt
     1081 tagtttgtat tctttttcat tttcgactaa atctactttt ggataacttg aaacacctaa
     1141 aaaatcattg aatgctggac taaacacgtc attgaaatca gggaacatgt cgcgaatgct
     1201 aggtacattt gccatttttg atcatccttt caataagtat tttttattta tccctattta
     1261 cgtaataact ctattttgaa aaaaatgcaa gtataatggt tagctttgtg aatatttcat
     1321 ctaatacgat acaaaggcta tctttctaat ttgaaaaagg gatcattaaa ataataagaa
     1381 gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc aaatatagaa aaattgaatc
     1441 tagaaaaccg cccgacaata aatttcatta actcagttga ccgaaaatga agtagatacc
     1501 aaaaatttat caagagagca agtagagaag tgagtatatt cataagagaa atagctgtaa
     1561 cgaattatta ggttaatgca aatgcttatt tatactccgt ataactttct cagaaaaaat
     1621 gataccaata atgacttgct agtactgtaa atccgtataa tatcatctaa tcaaaatggt
     1681 gttccatttg catctgtcat cgtaatcgat ccttcgggtg acactcgata aaaaccaacg
     1741 cttccagatc ctccttggga acggatttgt ttgctggaag ctgtgaacaa ataatctcga
     1801 tcaatttttt gataaaagct aataacaata tcgtcacttt gtaattcgga acggtgggca
     1861 ataagaaata gaagtgcttt gtcagagttt tctaatggaa gagaaggtac agtgctttct
     1921 tgcatttcag atgttgaaga aatgctaaac gactgaatac ttgattggct atgttgcgaa
     1981 tctacattac cagtagaagt attagtaatg gaagtagtag aagtactttt agttgaacca
     2041 acctcagttg ttgaaatggc tgctgaagat tctttaacag aattctttgt tttatatcca
     2101 caggcagaaa aaagtattac agttcccaac aagcctgcgt aaatcccttt atgcatgata
     2161 gctcctccta aaatagatta tctttattca gtatatcact taaacaaaga aaagcagatg
     2221 gtttaaaatg aaaaaacctt cccaataaag ggaaggttca tgaagataat aactttttta
     2281 ataattatag ctttctaata tctttcatca acttttctag taatcattgg gaaaaatttc
     2341 aagcagtaga tcgcacaaat acctacgacg atataaatga tcttcgcaaa aatagttgtt
     2401 gaaccaccag caatcatagc tactaaatca agttcgaaca agcctaccaa cagccagttc
     2461 aaccctccca caatcaataa agttaatgca atagcatcta aagttttcat tagaattctc
     2521 ctttcttaaa tgataaaaca attatatatc aaaaagagaa acattgctta tataacgctc
     2581 aggaatttta gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac
     2641 attttttagc tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg
     2701 gatttgtaag aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga
     2761 aaagtagtaa ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg
     2821 ttgaagcaga atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa
     2881 aacctactat ctttaaaagt cgatgtgtct tctaaaacgg acatagaaga agggatatat
     2941 aaagtaatga aacatttcaa gacgatcgat ggtctagtca ataatgcagg aataaatatt
     3001 ccttctcttc taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt
     3061 tttgataaga tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga
     3121 agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg
     3181 gaaggatcag aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact
     3241 cgttcttggg caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg
     3301 atactggaag aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga
     3361 ggtataagcg tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga
     3421 aggagtggta agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca
     3481 agttatatca ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata
     3541 ggggggacgc acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact
     3601 cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata
     3661 aaaaaagata ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa
     3721 aacaaacatt ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga
     3781 aaattgaaaa cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat
     3841 aggctgatcg aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc
     3901 agcagaggaa cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat
     3961 gtaaagatta caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta
     4021 cgactacttt tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt
     4081 ctagaagaaa aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa
     4141 atcacccttt taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg
     4201 ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa
     4261 ctgatcaatc agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt
     4321 atcagttttc ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct
     4381 gatttgcgct tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat
     4441 ctggatgagt ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg
     4501 gtaatttttc gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct
     4561 ttttcttacc aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag
     4621 atctctaaag tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa
     4681 aaagagcatt tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca
     4741 ttaatgatcc aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt
     4801 tcagaagaac aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt
     4861 ccgttaaaaa atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac
     4921 tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct
     4981 actgtttttt tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa
     5041 ttagtcgctc ctttgatcag aggctattat gtggatgaag aatttgaaaa aaggatcttg
     5101 aaaagagaat atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc
     5161 aaccagctat ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca
     5221 gaaacaggag gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa
     5281 gttgagaatg aattatttga gctatacgat atcctatttg aactcataaa aatgggaata
     5341 gatgagttta ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa
     5401 ggagagagac ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt
     5461 gctgcaagca gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact
     5521 gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat
     5581 tgttatgcta gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt
     5641 tacatctttg gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat
     5701 cgatcgttac catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa
     5761 tgccagagaa gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt
     5821 gccttttatt gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag
     5881 gagtgacaaa cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa
     5941 acaggtgcag aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg
     6001 atggttgcaa tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc
     6061 aaattatcag cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt
     6121 atgttgggaa atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca
     6181 agctattatg ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt
     6241 aatcctggag aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta
     6301 aattcgatgg agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt
     6361 ggttggataa ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc
     6421 aaaaaagtag agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta
     6481 agatcgtaag aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata
     6541 aacacaagtt catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag
     6601 aagaactgac aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa
     6661 tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag
     6721 ggataccaac catcaatatc gtttccacag gaaaaagtgg cccattagca caatatatca
     6781 ctgaagatat ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg
     6841 atgataacaa agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt
     6901 ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg
     6961 gcgcgggaaa agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga
     7021 tccggacagt gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt
     7081 ctgggatcgg taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat
     7141 taacaatcat tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg
     7201 cagttattag ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc
     7261 ctccccaatt agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta
     7321 ttccagtcgg tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat
     7381 cagttcttta ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa
     7441 gttttggatt atatagctga
//
LOCUS       DBKOIJ010000132         7259 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000132 DBKOIJ010000000
VERSION     DBKOIJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.134"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(39..863)
                     /locus_tag="ACV5NJ_002242"
     CDS             complement(39..863)
                     /locus_tag="ACV5NJ_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HHY6486290.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(836..1516)
                     /locus_tag="ACV5NJ_002243"
     CDS             complement(836..1516)
                     /locus_tag="ACV5NJ_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HHY6486291.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1539..2477)
                     /locus_tag="ACV5NJ_002244"
     CDS             complement(1539..2477)
                     /locus_tag="ACV5NJ_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HHY6486292.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2722..3573)
                     /gene="ispE"
                     /locus_tag="ACV5NJ_002245"
     CDS             complement(2722..3573)
                     /gene="ispE"
                     /locus_tag="ACV5NJ_002245"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HHY6486293.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3727..6300)
                     /gene="mprF"
                     /locus_tag="ACV5NJ_002246"
     CDS             complement(3727..6300)
                     /gene="mprF"
                     /locus_tag="ACV5NJ_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HHY6486294.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6441..7247
                     /gene="proC"
                     /locus_tag="ACV5NJ_002247"
     CDS             6441..7247
                     /gene="proC"
                     /locus_tag="ACV5NJ_002247"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HHY6486295.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         2219 a         1593 c         1250 g         2197 t
ORIGIN      
        1 aaatttttcg tttttataag gcttcttttc tttataattt agttattttt tcttctgaag
       61 gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa gactaatgtg
      121 attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga cagcatccct
      181 agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc taatcgcata
      241 ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc aatcgggatc
      301 atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa atgaacgggt
      361 agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg ccgtttgaat
      421 aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac ttgctcccat
      481 gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga attccctccg
      541 ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat ggcaatggag
      601 atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc tgctaataca
      661 acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa cgctacccct
      721 gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat cactaaaaac
      781 acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc tcttctcatg
      841 aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg atctgtctgt
      901 ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat ttcccatgga
      961 catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca tccatacctg
     1021 ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc gctaaactga
     1081 ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata tcccacatcc
     1141 ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga cgcttgaacc
     1201 agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta ggaaaacctg
     1261 cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt ccttcttgat
     1321 ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac gatgctttca
     1381 ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca cccgcatcta
     1441 catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc aaatcagata
     1501 tttcgatata acgcacctta caacctccat tctaaaactt aattgatact ttcttttaaa
     1561 gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat ttgttcatta
     1621 gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt cgacaatgtc
     1681 tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt gtttttcttc
     1741 acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg tgaaacgccc
     1801 gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc agcatgctga
     1861 gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg atccagtgct
     1921 tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt atctggatat
     1981 ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac cggatcgagc
     2041 caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc ttcatgttcg
     2101 tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat caaggtctgt
     2161 ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga actattatac
     2221 acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc gaaatcatgc
     2281 ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac gatctctttt
     2341 gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt tttgtctgct
     2401 tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac cgtagctgtc
     2461 aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt ttacgattta
     2521 ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc atcaatttct
     2581 gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg ctcatatcta
     2641 gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca gtttttcatc
     2701 acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt cctcacaaaa
     2761 accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca aggcaaaaac
     2821 ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca tacgctcttt
     2881 gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt ttcccatttt
     2941 tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt ggatgtcttg
     3001 atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga cactcattct
     3061 tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga tttcttcccc
     3121 tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg taccgacttc
     3181 caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt ggttgatccc
     3241 tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta ctggaatatt
     3301 cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta gcaataaagc
     3361 agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg tttctacaat
     3421 gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta cgctcgacat
     3481 caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca gtcctaaatt
     3541 tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa aagtctaagt
     3601 cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct agtggttgat
     3661 tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa caagttctag
     3721 tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc ctttttcttt
     3781 tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt tcctataagc
     3841 aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag cagactggaa
     3901 ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt gaaagaaaga
     3961 aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt ccgagtagcc
     4021 cattttctcg tatacaataa acagattggc taacagatag tcagctaatt cctcagattc
     4081 tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg gctgagcggt
     4141 gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag ataatacata
     4201 tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa gccatttttg
     4261 tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc aagtaaaatg
     4321 gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag acaagtcttc
     4381 tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac ccaattcatg
     4441 aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc ctaaacaatc
     4501 tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc caactggatc
     4561 tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt gatcttggtc
     4621 gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc ttgtagctgt
     4681 gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac tgccaagctt
     4741 cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg agatgcccaa
     4801 accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat gatttcctgt
     4861 tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa cgataaacaa
     4921 aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga attttgttcg
     4981 ataaaattct tttctggata accacacagc taacagaatc aagacatata ccaatatcaa
     5041 ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta aagcaatcgt
     5101 aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga gtaataaaaa
     5161 gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca ataatacttg
     5221 aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca tcatgatccc
     5281 cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa acagtcgcgg
     5341 aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca atagaagtcc
     5401 cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa ctactgctac
     5461 atcttgccga acaggcaatt gactcattcc taagatcatc aaaacgtcaa aagtccccat
     5521 tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca taggatagat
     5581 cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata ccgcaagggc
     5641 tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg gataaaactc
     5701 gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg ctaaaaaact
     5761 accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa taaaaaagac
     5821 atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata gtgcgacttt
     5881 tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac tagcccgcag
     5941 acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg ttacccacgc
     6001 agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta agactctgat
     6061 cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca gaatcatgag
     6121 caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc aactcatgcc
     6181 attagcaata tttgcgactt gatttgccac aaagactaac acggagccga aaaaaatcag
     6241 ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt tatttttcac
     6301 attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc ttatttattt
     6361 taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa agatatccta
     6421 atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat atggcacaag
     6481 ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg ttcgtttcca
     6541 gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat gcatgcaaaa
     6601 gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa ccaaaaatca
     6661 ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc atttctattg
     6721 cttcaggaaa atcattaaca gatttagaaa cagcgcttga agatcctcag gcctccatcg
     6781 tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg atttgcaaaa
     6841 atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa gcaatcggca
     6901 gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct ggcagctcac
     6961 ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg aatggtttgt
     7021 ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt caaaaattcc
     7081 tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct ggcggtacaa
     7141 ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc atcaaaggaa
     7201 tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga tagcaatgt
//
LOCUS       DBKOIJ010000133         7259 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000133 DBKOIJ010000000
VERSION     DBKOIJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.135"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..414
                     /locus_tag="ACV5NJ_002248"
     CDS             <1..414
                     /locus_tag="ACV5NJ_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HHY6486296.1"
                     /translation="LESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSD
                     YRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAK
                     TDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            490..708
                     /locus_tag="ACV5NJ_002249"
     CDS             490..708
                     /locus_tag="ACV5NJ_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486297.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            695..3373
                     /locus_tag="ACV5NJ_002250"
     CDS             695..3373
                     /locus_tag="ACV5NJ_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HHY6486298.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3364..3561
                     /locus_tag="ACV5NJ_002251"
     CDS             3364..3561
                     /locus_tag="ACV5NJ_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486299.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3565..4176
                     /locus_tag="ACV5NJ_002252"
     CDS             3565..4176
                     /locus_tag="ACV5NJ_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HHY6486300.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4161..5552
                     /locus_tag="ACV5NJ_002253"
     CDS             4161..5552
                     /locus_tag="ACV5NJ_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HHY6486301.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5768..6082
                     /locus_tag="ACV5NJ_002254"
     CDS             5768..6082
                     /locus_tag="ACV5NJ_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486302.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6075..6569
                     /locus_tag="ACV5NJ_002255"
     CDS             6075..6569
                     /locus_tag="ACV5NJ_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486303.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6637..6951
                     /locus_tag="ACV5NJ_002256"
     CDS             6637..6951
                     /locus_tag="ACV5NJ_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486304.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7050..>7259
                     /locus_tag="ACV5NJ_002257"
     CDS             7050..>7259
                     /locus_tag="ACV5NJ_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HHY6486305.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2774 a          985 c         1410 g         2090 t
ORIGIN      
        1 cttgaatcac tttcaactaa aaataataaa ttagatgaag aattgatcga aacatttatt
       61 gaagaaaatc ttcttaaaca aatatgggga gaaagtattg tgaattgctt aaaactggct
      121 agcaatagtg attatcgtca attcgacaac tggtacaaaa aattcaaata tgcgattaga
      181 tctgctgaaa aagaacaaaa aattcaactg aaaattatct atgaaatttg taataacaag
      241 tattttgtag atcatgttag agaacagcta tcaatgactt tacgtgactt gatacgaaga
      301 gcaaaaacag atcatcgtat caaacaaaaa gacgattata tctttgcttc tctaaaaaat
      361 aaagcgttag aactaattga attgcaaatc agcgaaggaa tagataagca ataagaatag
      421 aattgagtca aatgaaatgg aggggtatct ccacttcatt tgagaacttt gaatatagga
      481 ggggttattt tgattaaaaa tcgtttaata gatcaagttt ggtgtctaga tcaaaataca
      541 gatagtctcg aattaatcga gactatttgc ttcaacacta tagttctcga tttaagagct
      601 gaaaatgata attgtgtaac tcatgtcata attaatcaaa aaatggctca gaagctctct
      661 gagagcctca gaaaatgggc taagggaggt aacgatgaaa ataattgaaa aaaagattgc
      721 caataaaaaa ataaattggt tggttgttaa ttttcttgtt ctgatagcta tgattatttt
      781 tacaagtaat attgctcatg cgagtatttt caattggttt gatgacacaa aaagtcaaac
      841 agagtttttg aataattctc tttatagacc ttacctaaaa aagtacaatg gagatttcct
      901 tagtcagttc gggatttggc tcggatgggc tgtggtaaaa ggcatgttta ctgtgacaga
      961 ttcgattcaa aatatgattc cagatgtgct ggatttgttc aatttcattg aaagtacagg
     1021 gctaaataat gtttatcaat cagccgtaaa tacaattgtc attggattaa tgatcctttc
     1081 attgatgttt gttggttaca aaatgattac tggaaaagga acagtggatt taaaatcggt
     1141 tggaatgaac gtagtgatgt ctatagcatt aatactttta atgccaacca tgatttctag
     1201 tggtattcaa tttgctaaaa cattttataa ggactctact acgattacaa attcagatga
     1261 cggagtcgct tggtcattga taaaacaagg agtaacagat ttagcatata ttaataaaac
     1321 agatcagtac tcaagcattg ataaaacgga ggatcgaaat aatttaacac gaaaaaattt
     1381 tcaacagacg gatctcacac aagtgttaac ggataaagtg attgataaat tggaaaaaga
     1441 aaatccaatt gcggacaatc ttcgttatga attagttgaa aattctaata atgaatttgt
     1501 tgctacaaag ttttctgaca acttcttatc aacattttct gatagcttga aatcaggcta
     1561 ttaccgatac caagcaaatc tatgggggat ttcaattggg ttgacagcgt tagcaatcgc
     1621 ctatgtgttc agcgcgtttg tgatcattac agcaatacta gaacttgcat ttaaacgtgt
     1681 gttaggtgtt cttgtttttg caacagatat tgagacagga cagcgttcaa aagttgttct
     1741 atcagatatt ctacaatgtt acttaactgt tgggtttcaa ggatttgggt tatcaatgtt
     1801 tgctatgttt atcaattttc tgaactcagg acaaggtatc tcaacaaata tttttataaa
     1861 aacaattgct tatatctgtg ccgtttttgt tttaatcaaa ggcagtggaa cagtcatgag
     1921 atactttggt gttgatattg gactcaaaga aggctatggg caacttgctt ctgcttttgg
     1981 aatgggcgca atgctgttta gaaaaggatc aaatggattt aatcgtgcta aaggaagcgg
     2041 agaaggagaa gatcgaaaac cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg
     2101 caaaactgga agagctttgg gatatgccca tgaacgtgga ttatccggtt tagcttcaga
     2161 tggagctaca atggctagtg aaagagcaac aaaaccattt aagagtatgc gtgatatagc
     2221 taatgatacc aagaataagt ttaaagaagg tttagatgat ggtacagtgt ccgctattaa
     2281 taaaaatagc aaacctatgt tagctaaaaa taaagaagat gaaactggaa aatatgctga
     2341 ttccgtgcca acacgtcttt cagatagaaa agatggtgct aaagttgaaa atgccgatag
     2401 aattatgtct agttctgaac gtatgcgtga agcaatgaaa aataatgctg aatcgaataa
     2461 taatcctgca agcgcaattc agcaaaaagt ccaacaagat attgaagaac gtaaaaatgc
     2521 tatgcacggt caagctaagt ctgctgaaga attaattaat cagaagcgtc atgaagcgaa
     2581 atacacgcct gaagcaatga atcgcgagga aataatgtta aggaaacgtg tagaagggcg
     2641 gacaggcgct aatattgacg gaaaagaagc gttgaccaaa gaaagaattc aagaagctaa
     2701 aaattcaaat acaggattag aaaaattagt caaagaaaat ccacaaaatt ctacaagtag
     2761 tcatggtggc cgatcagtag atgtccgaga aaatattcaa ggttcactga atggtcatgt
     2821 tggacggaat gttgatgtaa gagagaattt acaaagttct tcaaatgctc aaggtactaa
     2881 gacaattgat gttcgagaaa atctgcaaag tagcagtaca agtcaaccaa aaacagtaga
     2941 tgttcgagaa aatgtacaaa attctacaag tagtcaaggg tttaaatcag tggatgtccg
     3001 agagaatgtg caaggtagca gtgcaagtca accaaaaaca gtagatgtcc gagaaaatgt
     3061 acaaagttct acaagtagtc aaggattcaa aacagtagat gttcgggaga acattcaaaa
     3121 agatagtgga gaaatgaaag aaaaaacaca aaaaatcaat atcgttgaag acagaaagtc
     3181 agcgaaaaaa tttgattcaa atcatgaaac agtgattatt gatagtgaaa tccgtgaaaa
     3241 tgataaggga tcaaaatcaa gacgaagatt cacttatgaa gacaatgagt tattcagaga
     3301 tacacttaat gatcctaatc cacttttcga taatttatta agaaataaaa gggggggatt
     3361 gtcatgcaat taattctaaa gattagtacg catgatttct tagaacttga cgaattatgt
     3421 gaatcagtaa agaacgcaac aaatgaaaaa atcacggtca ctaattatat tgaactgatg
     3481 attcaagaag aattaaagat gaaaaatgaa atagagagca atttcaaatg ttgctctcta
     3541 gaaaaaaatg tgaggggata atcaatggat gataaacaaa aaatgtacga aaaaaatcgc
     3601 aaacatagtg aggccttaaa ttttcgtgtc gataaaaaga cttatcttga actgacattg
     3661 attgcaaaac aaaggggaat ttctagagca gacgtttgcc gtgagcggtt aaagggactc
     3721 aaatatgaac cgccgttgtt agctgaagat gatagggtag ttttaaccaa acagttaaat
     3781 caaattggta acaatttgaa tcaagcggta aaggcattaa atatcattgc taaatatttt
     3841 agagaagaat ctgaagataa aaaagaaaag agcctacatt ttagttttga tgctagccgt
     3901 tacaacattt ttaaagatga acttgcgaat agcatagcaa aaaagttgat gattagttat
     3961 ccagatgaca tttttttgga gtccaatcgg agtgccccct ctttttttaa attatcagaa
     4021 gtagaaaaag aatatttcga atattggata gaaaagcaaa ctagaccggg tacgaaagaa
     4081 gagtattccc aaaaagttga tcggaattta attcactcaa ttcagattat tgcaagttta
     4141 aaagatgagg tgaaacaact ttgggagcta ctataacatc gaaaggacgc aagaaaaatt
     4201 tatcctcgct attacaatat gcagctaatc cttcaaaaaa agaaaaatgt gttgcagtct
     4261 caggaataaa ttgtagtgat gatagtgagg aagcactgca agaaatgaat ttagtacgta
     4321 ccaagatgaa taaatctaaa gatatagcac aagcgtttca tgttattcat tcgtttgata
     4381 agagtacttc taaagagtta agtattgata aaatgcacga aatagctgtc gaatttgcag
     4441 agaaagcttt tccaaattcg caaatcattg ttgcaagtca caatgataaa gatcattttc
     4501 atagtcatct tgtcattaat aatattaaca tggaaacatg taaaaggttg cgtattgacc
     4561 ctaaagattt agaactaatg cgaaaaatta acgatcaagt cctcaatgaa catggtcttg
     4621 aatcagtgaa taatgagtac tatgaaaact tagaaaagag aaccaatctt tattcaaacg
     4681 atccttctaa gatgaaatat ggaaaaatta attatcaaga gtttgtgcaa aatgcgatcg
     4741 aagcaacgtt ggaaaaatgc gaagtgaatt cttttgaaga tttttcggat catttggcaa
     4801 aagaatataa cattgaagtt tatcaatttt caaaaacaaa taacaaactt ggctatgttc
     4861 tttacaaaca aggtgtaaat ttctatgagc gtagaggtga gttaattact ttaggaagag
     4921 ataaacaatc tagaaaagaa aaaagtcagt atgtcgaacg tacttttagt gcaagaaagt
     4981 tagggaaaag atattcattt gaatctgttt ctgaacaact tgaaaagaat ttagaagaat
     5041 cacatataaa aaattttttt aatatggaaa gagagcaagt tatgtcgcta attagtggag
     5101 taaattacaa aactggtgag ttaaatgtac cgtttgtgga attagattcc atcgcacaaa
     5161 agcaatttag cgaccgtgaa ggcgatgaaa aattcattaa atatgatgac ttcttgcgag
     5221 aggtagacaa gcattttgta aatacaatgt ctttagaaga tgatcaagaa tacacgatta
     5281 aacttcatac ggatgtgaat cctcaaactt tacgtagtgc gaaaagtaaa acaatttcta
     5341 ttaaaaagac tgatcagaaa gagttatttc taccggcaaa gggttttgaa aattgttctc
     5401 atacaccttc tgctaaacag attcactatc aaatcagtcg cataatgaat gacgatgtaa
     5461 aaatgtatcg tgctaaaaag gattatgaat tcacacaaca aatgattgaa aatcagcaaa
     5521 aaaggcaaca aaattttgaa atggaattct gaatgggctt gttagtggac tttccagttt
     5581 tttgcctatg aagagttgct tggaaagaag cagtttttag gacatacgcc agctacccat
     5641 ctacttgcca gtcaatccgc cccgctcacg ctaggctact ttctggcaag tcagatggcg
     5701 ggtaaatctc tgagggagca agctccctac agactccctc ccaaaaaaag aaaggtggaa
     5761 gaaaaaaatg aactatccat attttaaagt ctcggcttca gaagaaacta aagaaatatt
     5821 caacaatttt tacaaccaaa acaaaggaat ttttggttca aaggcaaata tgtttcgtgt
     5881 tatggtttca aatttaccag tgttagctag tccatcaaat aataaattta atgatcctga
     5941 aagcattaaa tttgaacaaa aaatttcaga actagaatca atgataagta atgaagtaat
     6001 tgaaaagctg gatgacattg atcaaaagtt atcgtattct ctaaaaaaca aatacaaaac
     6061 ggaggagaaa aaagatgttt aaagtttact tcccgtttca gtggctcgtt aaaggaattc
     6121 ttttagtgct actaggattg attctttttg gaagttatct aacaattaaa gccagacatt
     6181 ttccagaaga aaagccaata aaaattgtaa caaaagattt aaaaataggt gaaacttatc
     6241 aggcagatgg ggttgaacta acatttaaaa atattgaagt gaccgaaaaa agaaatgaat
     6301 ttgcacaaca tgatcgtgta ttacaagtga cctatacact tataaatgat acagaaaatc
     6361 cgatacgtcc acaacaatgg tcgattgaaa ataaggatgg tgaagaactt tatgagtatg
     6421 atatgatatt ggataatcca gagtatattg aaagtggcaa agaagaaaca gtaaattact
     6481 attatgaatt accgattgaa aggcaggact accaaatcgt agtacgtaga gagaatggtt
     6541 acatagttgg gttgccattt actttataag ttttattttg ttagtagttt ttaaggtaat
     6601 atcagttttg tgatttttga aaatggaact gggagaatgg ctgaggaaaa taaagttgtt
     6661 ccgtttgaaa cattagtgat aaataaggaa gagtttgagc aactttttct tgattttttt
     6721 attcaatatg acatgattta tgattttagt tcgtcactag atttatttga attagaagca
     6781 gaaataatag aaaacagtcg agagaagaat ggtaaaatca ttatttcagt agaaaaattc
     6841 aatgaattat tctcacaaac atttgaaata gatccagact attatcaacc acaagaagtt
     6901 tttcgagagt ttagaaataa gattattggt tttagtcaaa ctacaaaatg aaagcttaat
     6961 aaagcaagtg ttgatagaat tattcctcaa cgcttgcttt attttatggg cacttcataa
     7021 tagaggtata gagaagagag gtattcattt tggatactat tactccaaca aacttcaaag
     7081 aaaatatagg taaagtgttg caagaagtag ttcattcaaa ttctgaaata gaaattacta
     7141 gggattctaa aaatggattg aacgatggcg tcattatgat ttctaagaga aaatgggagt
     7201 tgttacaaga agaactttat ttacaacgaa cgggtacatg ggcttatgta tctgattta
//
LOCUS       DBKOIJ010000134         7194 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000134 DBKOIJ010000000
VERSION     DBKOIJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.136"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(42..1168)
                     /locus_tag="ACV5NJ_002258"
     CDS             complement(join(42..940,940..1168))
                     /locus_tag="ACV5NJ_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHY6486306.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1572..2354)
                     /locus_tag="ACV5NJ_002259"
     CDS             complement(1572..2354)
                     /locus_tag="ACV5NJ_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486307.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2419..3255)
                     /locus_tag="ACV5NJ_002260"
     CDS             complement(2419..3255)
                     /locus_tag="ACV5NJ_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HHY6486308.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3273..4682)
                     /locus_tag="ACV5NJ_002261"
     CDS             complement(3273..4682)
                     /locus_tag="ACV5NJ_002261"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HHY6486309.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4696..6519)
                     /locus_tag="ACV5NJ_002262"
     CDS             complement(4696..6519)
                     /locus_tag="ACV5NJ_002262"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HHY6486310.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         2190 a         1498 c         1158 g         2348 t
ORIGIN      
        1 ggatttgagt caatatcttg gacacatttt agtagagacg gttaagccat tttttctttt
       61 gctaattctt caaattcctg tggggttaga tagtgaatcg agctatggat tcgatttcgg
      121 ttataaaatc cttcaatgta gctaaatagc gctcgatttg cttcttcaaa atctgagtag
      181 ctagtcgtgt agacttcttc ttttttcaat gaggcatgga aagattcgat tccagcatta
      241 tcataaggtg ttccttttct actataagaa tgccttattt tgttggtttt aagccattgt
      301 tccacttctc ttgctgtgta ttggctgccc aagtcagtgt gtagaatcat tccttctgga
      361 atgtgataat gtatttttgc tttattcaat gtttgaatga cacaatccac agtcattcgt
      421 tttgaaaatg tataactaat aatttttttc gtgtgtaaat ccatgattga agataagtaa
      481 caccaaccat ttttcttagt aggaatgtac gtaatgtcag ctgcccattt ctcacagatc
      541 gtttcagtag aaaagtcctg atttaagaga ttctcttttg aaatgatcgg tttattagac
      601 ctttgaggtc tatatttttt aactacaatc gaccttaaat ttaattgttt cattagcttt
      661 tggacatgtt taagagatac tgaaatccct tcttttaata aaacttgatg gatctttgtg
      721 gcaccatagc gttgtttatt cgtaaaaaaa attgttgaaa tcttcttttt caacttatta
      781 tttctgattt ctgtagctgt caacggttta ttcttataaa aataatagac acttctaggg
      841 atttctaata accgacataa caaagaaaca gtgtaatcct tacaccattt ttcaataaat
      901 tgaacaagcg aatttaggtt ttccgagaga atatggctgc ggcttttttt aagatttcaa
      961 gttcctcatt taaacgagca ttttcttttc tcagttcagc tacttctttt ccagtcagtc
     1021 ctgtggactg atcaggtaaa tataaattct tccatttgta aatcgttgct tcagataagc
     1081 catattcttt cgctaaccct cgaacagaac gtccagtttg attcagttca acaatcattt
     1141 gtttaaaatt ttcttcatat cgggtcataa aaattcatcc tctcgtagat aagtttaatt
     1201 tattcttgtc tctacgaaaa gctgtctaag atttgatact aacaccaagt tatctttaga
     1261 accatttcag atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt
     1321 ctagtagtat aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa
     1381 cagtctaaaa cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac
     1441 aaataaatat agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc
     1501 tactggagat gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga
     1561 tggaagaatc attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg
     1621 ttcttaaata ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatgtct
     1681 ttaccaactt gctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat
     1741 aaagattgag catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca
     1801 tttacaagct tccaagtagg tggtatagct gtgtatgttt cgatgaatga atcaattctt
     1861 tcatctatcg tcttcccagt atctacttct gtctcattac tcttagatga atcgattctt
     1921 tcatccatcg tctgctccgt aactgcttct gtctcattat tctcaccgga aactctcttc
     1981 acacccaaac ttatatattg tccatcttta gtgtgcatag ttaaaattcc cacgctttta
     2041 ctaataaatt cactataacc agttctttca atagagactt caatgtattt cacactatca
     2101 tactcaaaag aaaaatagac atgtaaaagc attttgttgt ttacatctac ataataatcc
     2161 tttgactcta cttgagacct tcctatacgg aatacagctt tactgtctga tatctctata
     2221 ccagcatcct tattattaat ctgccaatct ccaagtagct cttctggcaa gctagtagtt
     2281 tcttgtgaac actttaccac ttcactggta gaggtcgaaa cattcacctc cgcactgtta
     2341 gaagaatttt ccaaggaatc aggagcctta gcacaaccaa caagtcttta tatattgtgc
     2401 agccgatatt ttcttcattc agtacaccct tgaaatatgc agtgttagat aaagcagttc
     2461 gtcctgattt gctgaggcac ccatctcttc ttgaacgtac tccttgattt tcaaggccgc
     2521 ccgatactct ttcggaaaac gctgcttcac tgaagccagc aaaaaatcat ccttctccgg
     2581 caactcttcc ttcttaatca ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa
     2641 gtaaagcatc gaatctttat caaccttttt ttcgggaaac agatatccta gcatagccaa
     2701 gatatccttt agtgctctta ccgagagcat ggtttcttcc atatttgctt gttcactttg
     2761 ggcatttaca atgtgaaacg caatgtttcc agcctcctct tctggcaact gaacattcat
     2821 ttttttgttg atcaattgga tagcatagca gccgatctca aattcttttg ggtaatactt
     2881 ttgaatttca atcagcaggc gattttgaaa aacaatgcct ttttgactgc gttcaatggc
     2941 atgcttcaag tgatcaacca aggtaaaaaa tagactttta ttcagcttaa agtcaaactt
     3001 ggcgttggca taatcgataa ttcgcttgac gcaaataatt agatcatccg gagcagcctc
     3061 aaacagttta atgtaatcct tttttagagc atcagaaagt gaatctaatt taaatacttt
     3121 ttggattttg tcagccggaa ccaaatcgcc tttctttagt tgaaatccaa tccctttccc
     3181 catcaaaatt acttctttgc cttgcttatc taagcaaaga attaaactgt tgtttaacgt
     3241 tttaattact ctcattagat tcaccgttct ctctaagcta agccatttct tgcgattact
     3301 ttttgatacc aaaagaagct ttcttttttt attcgtttta attctttaag attctcatcg
     3361 gttcggtcaa catatataaa accataacgc ttattgaagc cttgatgggt actaacaaca
     3421 tcaatcgctg accaaggtaa ataaccaaaa acatctacac catctgttaa agctgcttga
     3481 atagccagaa tatgtttggc tagataatca attcgatcct ggtcattaat tcgtctatct
     3541 tcctcaacta tgtcgggtgc cccatagcca ttttcagtaa ttaaaatagg taaatcatag
     3601 cgatcgtaca atttcctcaa tgttaatcgt agtccagtag ggtcaactac ccagccatat
     3661 ggcgttttat ctacataagg attttcagct gcccggtaga caccttcttc tcccaacatg
     3721 atctgttgat cccccgctct agcggaaaca tctgatccgt catttttgct ggcagcgatg
     3781 gtggcagttg aataataatt aatcgcaata aaatcgggtt taccggagga taaaattgcc
     3841 tgatcctctg cctcaatttt aggatataat ccacgatcat tcaaatagtt ttcaaacaaa
     3901 taattgtatt ttccccgaac tgctacatct aagaaagacc agcctctcaa tgtttcccaa
     3961 ttatgggctg caattgcatc ttctggactg gaggtagctt gatacatagc cgttaaattg
     4021 attgctggac cgatttttcc tttaagattc aactgatgat acaagtgaat tactctagct
     4081 tgagctaata aaacatggtg attaatttga aagagttctt tttttgaagg cagctctcca
     4141 cttggtggca aaccaatcgc tccaggatga aggatcatcg tgttttgctc attaattgtc
     4201 agccaatagt tcaccttgtc accatactca gtaagcaata cctgtacata gtccacaaaa
     4261 taatcgatag ttgctcgatt taaccaaccg ccctgctcct ctagaaaata aggtaaatcg
     4321 aaatggtaca tggtgacaat cggttcgatt ccctgattca ggcattcatc aattacttga
     4381 tgataaaaag aaattccctt ttgattggtt ttattatttt caatatccat aattcgcgtc
     4441 caagcgatgg aaaagcgaaa agcttttaag cctaattctt taaacagctg tatatcttct
     4501 ttatagtgat ggtaaaaatc cgaggcaact tcaaaattag ctaccccttc tgtatgctga
     4561 tacttgtcaa taatagacgg ttgtttccca tcctcctgat aggcaccctc tgcctggtag
     4621 gcactaatgg aggcacccca taaaaacttc tctggaaatg ctgaaagctg tttgtattgc
     4681 acaaaaatcc tctccttaac taatttgcat caatggttcg tgttccttta agcttccgct
     4741 tgcttcacta attcctgcgt aattttcact gttggtcact accacaatca ctgtatcatc
     4801 gtagccagcg tttctaatgg cagcgaaatc ggcgttgatt aataattgtc ctgctgacac
     4861 ctgatctcct gcttttacaa atacttcaaa gggttcccct tttaaattca cggtgtcgat
     4921 ccccacatgg atcaataatt caataccttc tttagaacgt agaccaatcg catgtttaga
     4981 ttctaaaata gtaatgactt ctccctcaac aggtgaataa atgctgtttt cttctggatg
     5041 aatggctacg ccctttccca atagtccctt agaaaagaca gcatcgttca cttcagaaat
     5101 aggacacacg gttcctttta ccggattttc aacaatgact gcttcggcta ttgccgtttg
     5161 ctctgattca tcagaagtat cgtcaaatcc aattagataa gcaatgataa aggttgcaac
     5221 aaaggcgatt acacacgtaa taattgcgtg aacaatattc attggatttt ctccaataaa
     5281 ggctggtaag gctgctaagc caggggaaac aaaggcatat ctgactaaac cgctcaagcc
     5341 agcatacaaa ccagcaattg caccaccaat catggaagca actaatggtt ttttcagctt
     5401 caaggttact ccatagagtg aaggctcagt aattcccatt aaagcagtga tcccagtgga
     5461 cacagctagc tgcttcaact ctttgttttt agttttcgcg gctacagcaa aggtagcacc
     5521 tgattgagca atattagaag ccagcattcc tggcccattg atcatttcag aacccgtact
     5581 gctcaattgc atagtagcga ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa
     5641 tggctgcaaa ccacccatca aaaatggaat taaccaagct gctttgccgt taatccattc
     5701 agcccccatt gctataagat cgttcagcaa cgttccaaac ggtccaatga caaccaacgc
     5761 taaaggcccc ataattaaaa ataccagcat cggcttagta aagaatttaa taaacgaagg
     5821 cgaaattcga tccgcaaagg gctctacata ggacatgatc caaacagtaa agataatcgg
     5881 taaaactgat cccgcataat ctgcagctaa taccggaatt cctagaaaag aaacggcatc
     5941 gccagtagaa aaaagctgtg aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc
     6001 caaaattggg ttgacatcaa actttatggc tgccccatag gctaatagta ctggcataaa
     6061 ataaaaggct gcatcagcaa tagtattcaa aatgatgtaa gtttgactac tgtctgatag
     6121 tacaccagtc aaagtcaaga cggccaaaat ggcttttacc atcccggcac ctgttatagc
     6181 tggaatcatc ggtgtaaagg tggtagaaat gacactaatc aatcttgaaa taatactttc
     6241 ttgattgtct gaagaggggc cctttttaat tgaagttccc acttcctttt gaatttcctt
     6301 gaaggctgtt tggacttcat tgccaatcac cacttggtat tgtccaccgc tatccaccac
     6361 actaattaca ccaagtgttt tttctaaggc tttcgtatcg gctttccttt tatctttgag
     6421 ttcaaaacgc agtctggtgg cacaatgggt taagctgacc acattttctt ttcccccaac
     6481 ctgttgcaat acatttcttc ctacttctct gtaatccatt attctctctc cttaaagcag
     6541 taaaaaagac caagtcaaac cacgccccaa tcaacattga agtgttagtt tgtcttggtc
     6601 ttgcctgcat aatcagtaac atgccaataa cgttattttg ctgtaatcca ataatagcac
     6661 attatgatag cgcaatcaac agggaattta attttttatc ttttcgtttg accgtatagg
     6721 gtaatataac agttagatca catcaactag ggtatatctg cgtatactta ctgctaaaat
     6781 aagtatatct aatattaact ataccagtaa aaccattcct acataaccag cgggaaattc
     6841 ccaaaatcgt gaaagcatca aaaatgcata taacggatca cacaacttca acaattctgc
     6901 tcttgggaaa aattgatgaa tttggtgagt aaaggatgga ataaacagca ttccaacaat
     6961 ataggcaaca atagtgatac ttgttttttt ggtaaaaatc agtaagcaat agccaatttc
     7021 aatcattccg agatataaag gtatcgatag aatgatttgg gctagtattc ttaaaaatta
     7081 actagcaaat gtttaaactg atttatttct aatctacatg tagtaagtat ttcaattcca
     7141 ccaattatca ataaaaatac caaacatatc cataataatt ttttcttttt catt
//
LOCUS       DBKOIJ010000135         7171 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000135 DBKOIJ010000000
VERSION     DBKOIJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.137"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(307..1254)
                     /locus_tag="ACV5NJ_002263"
                     /pseudo
     CDS             complement(307..1254)
                     /locus_tag="ACV5NJ_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(1254..1871)
                     /locus_tag="ACV5NJ_002264"
     CDS             complement(1254..1871)
                     /locus_tag="ACV5NJ_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6486311.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(1885..2055)
                     /locus_tag="ACV5NJ_002265"
     CDS             complement(1885..2055)
                     /locus_tag="ACV5NJ_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486312.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(2403..3893)
                     /locus_tag="ACV5NJ_002266"
     CDS             complement(2403..3893)
                     /locus_tag="ACV5NJ_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HHY6486313.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(3936..4235)
                     /locus_tag="ACV5NJ_002267"
     CDS             complement(3936..4235)
                     /locus_tag="ACV5NJ_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486314.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(4601..4885)
                     /locus_tag="ACV5NJ_002268"
     CDS             complement(4601..4885)
                     /locus_tag="ACV5NJ_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHY6486315.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(5404..5889)
                     /gene="vanZ-A"
                     /locus_tag="ACV5NJ_002269"
     CDS             complement(5404..5889)
                     /gene="vanZ-A"
                     /locus_tag="ACV5NJ_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HHY6486316.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(6042..6953)
                     /locus_tag="ACV5NJ_002270"
     CDS             complement(6042..6953)
                     /locus_tag="ACV5NJ_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HHY6486317.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2114 a         1350 c         1068 g         2639 t
ORIGIN      
        1 aaaattgacg aagggggttt tttggcgcac gcttttcgtt agaaatatac aagattgaaa
       61 atcgtgtata agtgcgccct ttgttttgaa cttagcacgt tacatcaatt ttttaaaatg
      121 atgtataagt gcgccctttt aaattttgag tgattatatt ttttgagtta gaaaaaggga
      181 ttgggaaaat ttcccaaaat aatttaaaaa ataagcaaaa attttcgata gagaatgtgc
      241 tattttttgt caaaggtgta taccttgact gtgcttgctg ttacattaag tttattttta
      301 agttattaaa aaagaaatag cttttaaagt ttggctcgct gtcgctttat aaagctgatt
      361 gacttttgat tgcaaactac ttaaagaaaa caaactcgga ctattcgttt tcttctcttt
      421 ggtttgaaca tcagcaatta tcccctcttg attgcctatt ttagcttgtt tagaagaaac
      481 aaaagctaaa agctcctctt gggttttaaa acgctgtgtg gggcttagaa cgcccttaaa
      541 cgacccttgg tttactttta tactagcttc cacctcgaaa aaaggttctt ttttaaaatt
      601 ctctatggct tcctggcgct gaaaaataag gtataaggtg ggcgtttgaa cacgtcctag
      661 tgaaaatgta ccttgtacgc ccttctgttg taaatttaac gtatacaaag ggcttgcgtt
      721 catgccgatc aaccaatcgg caatttggcg tgtttgcgct tcttgataaa agggatagta
      781 attcattcca ggttgcaaat tttgaaaacc gcttcggatt acatcttttt ctaagctatt
      841 gatccatagt cttttaaatg ttttatcttt tgaaaaggca tttgctttat ggataatcga
      901 ccaggcgata ttttcacctt ctctgtcgct atctgttgca acaataattg tatttgcctt
      961 tttgagaagt tctgcaacaa ttttaaactg ctttccctta tcttttgcaa cttcaaaatc
     1021 gtatcgatca ggaaaaatcg gcaaagattc aagtttccaa ttttgccact tttcgtcata
     1081 atgacctggt tctgctaatt ccactaaatg cccaaaacca aaggtgataa acgtttcatc
     1141 tgtaaatagt gggtctttga tctcaaaata accgtctttt ttggtgcttt gttttaaagc
     1201 acttgcgtag gctaatgcct ggcttggttt ttcagctaaa ataaccgtac tcattaacta
     1261 tccctctttt cattgttttt tctttgatcg actgtcacgt tatatcttgc tcgatacctt
     1321 ctaaacgttc ggcgattgat tccagtttgt tcttcaactt ctttatcgga taaaccattc
     1381 aaaaacaaat cgaaagcatg ctttaaacgt ggatcatttt ctttaaattt atgctgacga
     1441 cctttgaatt tgcctttttt cttagcaatt tcgattcctt gtgcctgacg ttccttaatt
     1501 ttttttcgtt ctgattctgc ttgatacttg tacaattcaa tgacaaggct attaatcaaa
     1561 cgccttaaat tttcatcttc aataccattc attgagggta aatttaagac ttccagggtt
     1621 gcccccttaa tttgaatttg attcatcaat tctgttaatt ctttattatt tcgtcctaat
     1681 cgatctaatt cagtaacaat aacaatatcc ccttcacgaa tatagttaag catagcttgt
     1741 aattgtgggc gttcgaccga ttgaccgctt aatttgtctg aaaagacctt agaaacgccc
     1801 tgtaacgctt gtaattgccg atctaagttc tgttctttgc tactgacacg tgcataacca
     1861 attttagcca ttttcaacca acctctaaaa ttctctcggt tgcaataacc aatcagcaat
     1921 atctactttt tcaatttcaa attgcttatc agaaattgtc ttttcgtaag cgataaaatc
     1981 ttgcgcatat tgttgctcat taaaaatagc caccacttcg tcattttcta aaactcgata
     2041 aataaatttt ttcattttac tcctcctatt atgcccaact taaatgacct attcaccaag
     2101 tcaattatac tgctaaaatc atattaggac aaataggtat actctaatga cctataaaag
     2161 atagaaaatt aaaaaatcaa gtgttcgctt cgctctcact gcccctcgac gttttagtag
     2221 cctttccctc acttcgttca gtccaagcca actaaaagtt ttcgggctac tctctccttc
     2281 tccccctaat aattaattaa aatcttactc tgtatatttc tgctaatcat tcgctaaaca
     2341 gcaaagaaaa aacaaacacg tatcatagat ataaatgtaa tggcatagtg cgggttttat
     2401 tttcagcctg tatcatagct aaacaaatcg agttgtgtgt ccgttttagg gcgttctgct
     2461 agcttgttta aagtctcttg aatgaatgta tgctctaagt caaaagaatt tgtcagcgcc
     2521 tttatatagc tttctttttc ttcttttttt actttaatga tcgatagcaa caatgattta
     2581 acactagcaa gttgaatgcc accatttctt cctggtttaa tcttaaagaa aatttcctga
     2641 ttcgccttca gtaccttcag caatttatct aatgtccgtt caggaatgcc tagcacttct
     2701 ctaatctctt ttttggtcgt cactaaataa ggcttgtata catcgctttt ttcgctaata
     2761 taagccatta aatcttcttt ccattctgac aaatgaacac gttgacgttc gcttcttttt
     2821 ttcttgaatt taaaccaccc ttgacggaca aataaatctt tactggttaa atcacttgat
     2881 acccaagctt tgcaaagaat ggtaatgtat tccctattag ccccttgata gttttctgaa
     2941 taggcacttc taacaatttt gattacttct ttttcttcta agggttgatc taatcgatta
     3001 ttaaactcaa acatattata ttcgcacgtt tcgattgaat agcctgaact aaagtaggct
     3061 aaagagaggg taaacatgac gttattacgc cctattaaac ccttttctcc tgaaaatttc
     3121 gtttcgtgca ataagagatt aaaccagggt tcatctactt gttttttgcc ttctgtaccg
     3181 cttaaaaccg ttagacttga acgagtaaag cccttattat ctgtttgttt gaaagaccaa
     3241 tcttgccatt ctttgaaaga ataacggtaa ttaggatcaa aaaattctac attgtccgtt
     3301 cttggtatgc gagcaatacc aaaatgatta cacgttagat caactggcaa agactttcca
     3361 aaatattctc ggatattttg cgaaattatt ttggctgctt tgacagattt aaattctgat
     3421 tttgaagtca catagactgg cgtttctaaa acaaaatatg cttgataacc tttatcagat
     3481 ttgataatca tagtaggcat aaaacctaaa tcaatagcgg ttgttaaaat atcgcttgct
     3541 gaaatagttt cttttgccgt gtgaatatca aaatcaataa agaaggtatt gatttgtctt
     3601 aaattgtttt cagaatgtcc tttcgtgtat gaacggtttt cgtctgcata cgttccataa
     3661 cgataaacgt tgggtgtcca atgtgtaaat gtatcttgat tttcttgaat cgcttcctcg
     3721 gaagtcagaa caacaccacg accgccaatc atgcttgatt ttgagcgata cgcaaaaata
     3781 gcccctttgc ttttacctgg cttggtagtg attgagcgaa ttttactatt tttaaatttg
     3841 tactttaaca agccgtcatg aagcacagtt tctacaacaa aagggatatt cattcagctg
     3901 ttctcctttc ctataaatcc tataaaatag gttgtttaat taacttggtt tgctttttca
     3961 ttcaactgtt tcaatattgc atgttttgaa aaagattttt ttcctttata agtcaatttt
     4021 tttccactaa tcgaataaat tattttgtta ttttctatta acttatatat ataatcttcc
     4081 ccctccgaag aaaaatactt atctgatttt gtttctaagt agatatttct cttttctaac
     4141 tctttcttaa acgtttctag tgtatagata tttgctaatt ttcttatctc caataaacta
     4201 ttttttatat aagttttaca ttcatcatga ttcatacaaa ctccaccttc tataaatgaa
     4261 tacaaaaaaa gcaatcaaac gatttccgat tgattgctta acaattctta aattcagtag
     4321 cttagatact tgaaaactct ctgatttccc tatataatga tagtacggtt atataccgtc
     4381 ttcaaacaaa gttaattaaa taacttctta cgagggaaga gttcatctga ctaactgata
     4441 agcgttggtt tggcaatctt atcgggctat gcatttataa aatgtcgtca aacattttat
     4501 aaatgtgtca tggctctttt ttcgtttcta ttcagttcgt tgtttcgtta tatctagtat
     4561 accgctttta aaaaaaataa gcaacgattt cgtgcattat tcacacgaag tcattgcttt
     4621 tttcttcttc catttctaaa tccaatgtta cttgttctga ttctgtttct ggttctggtt
     4681 ctgttggctc atttgggatt aaatccacta ctagcgttga gttagttaac tttgcaattt
     4741 gttctagtgt ttttatggtt ggatctgatt ttcctgattc tattcgtgaa taatttgatc
     4801 tactcatttc taattcttgg gagaattttt cttttgattt ggtacctctc atctttttta
     4861 agctttctct aaatgctagt tccaagtttc tcccccctct ttttgtgtaa taaaattcac
     4921 gctttattta aattatagct tttaatatct taataaacaa gtgattaatc acttgtgatt
     4981 tgtgattatt ccaaaagtag gtaatttcac caaccttttt caaaagtttt tatcgacgat
     5041 tattggactg gatcgtgttt cctaaagcga ttctaagcgc atgtttaatt ttaaagtggg
     5101 taattctctt taaaacgtcc tacaaagatt caaaaaaagc atggctgcta gtgggggtac
     5161 cgtcaggaaa atgcggattt acaacgttaa ggaaatttat taaagagacc aattcctcag
     5221 cactaaaatt gataattggg ctttctttgt tactatagtg ttaggtaagc tttttacttt
     5281 atcaccggtt ttcccctcac ttcacaccta cgggcgagtt tccccacatc ccgttttcca
     5341 tctaatccaa ttcattcaat atctttatgt gtatgaaaga tcagattgct tgatttagac
     5401 atcttacacg taatttattc ttaaatgggt acggtaaacg agcaataata cgagcaaaag
     5461 tatacctaca aaaataataa ctctgtctaa ttttttttga ttcatatgct tattgcttaa
     5521 accatataat ttcagtccaa gaaagcctcc aacagtattt gtaattacat ctgttatgtc
     5581 tgtcgctcca atagcgaaga taaattgaat tatttcaaaa gtaagactta aaaccagtac
     5641 aaaagcaaac ttaggtaaaa atccgatttc tttaaaattg acattcaaaa gcaagccaaa
     5701 tggaataaag attataacat tatctatcat ctctctgaaa ttcccagtag cagtaaatgg
     5761 agtcaagtta agacttcttt gatgataatt aaatactgat aaaatattgt attgtaattt
     5821 gaataacact aaccagatta gtgtcactaa atataaagct agcaatcctc tagataatat
     5881 ttttcccaat ataatttcct ctaattcttt tgtttaagtt gtatcattag tatagcaatg
     5941 tcctagttaa cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt gctagtacaa
     6001 taatgctgtt cattaatttc gttggtttca ttccgccatc cttacctcct tgaattagta
     6061 tgtgttgatc cgggaaacac tgtcacaatt acaccgtcta tattgtttcc tgatatctca
     6121 taacgaagat tagtcggcac atgaatggtg gtatttttag taacaggata ataaaagatc
     6181 tcatattttt ccccatttac actaacagaa atggtttttt cttcttttag gtaatccata
     6241 tattcctcga gaacgaaatt cttttctttc ataatcgcac tatgtggtaa accaacatag
     6301 cgaatatgcc atggttcata ttgaattcct gttaactctg ttttgtcctc tggataacgt
     6361 aaaatgaacc cgtatttcca agcattttct tctatccact ttccttcagg ggctcgttcc
     6421 attttcgtca agcttgatcc tacatctagt gataaacctg aattatgctc actataacct
     6481 gctggtaagg catactcagc ccccatttct tggtaaagca cactttgctc atcaaagtct
     6541 cgatagccac tattaataat aaaatgacta acgccaccct ttacagcatc attgaccatc
     6601 tctgaaaatt tttgtgctat ttcttttgac atataaatat tactatcaag caacccgtat
     6661 ccatttatta attcgtcatg tttagataaa ttcacgatat ctgacttcac actttcttgg
     6721 cgaacaggat atttactatt gattaatagc agatttcctt gataaacctg ttcttctgta
     6781 attgttttct cttgggtatt ttcagaagtc ccactatttt ctaaatgttc tttgggattt
     6841 tgatcataat tttgaaattc gactttttcc tgagaaaaca gtgcttcatt aacgtagtca
     6901 taacctaagt atattaagaa taacaataac aataaaaaaa acaacttctt cattttcagt
     6961 ctcctcctta actatatagt tggaagtatg aaatatttct agaatcttcc ccttaaacgg
     7021 cacaatctcg tttgagattg tgccgttttg ccccgtaaat tacagtcaat tatttcgccc
     7081 tactggattt actattatca ccaatgtaga tgaattttca taagtgatac ccatgctgaa
     7141 tttaacgagg ctgaactgcc tgttgacaat g
//
LOCUS       DBKOIJ010000136         7115 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000136 DBKOIJ010000000
VERSION     DBKOIJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.138"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            151..408
                     /locus_tag="ACV5NJ_002271"
     CDS             151..408
                     /locus_tag="ACV5NJ_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486318.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            521..1132
                     /locus_tag="ACV5NJ_002272"
     CDS             521..1132
                     /locus_tag="ACV5NJ_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486319.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            1311..1868
                     /locus_tag="ACV5NJ_002273"
     CDS             1311..1868
                     /locus_tag="ACV5NJ_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486320.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(2567..3088)
                     /locus_tag="ACV5NJ_002274"
     CDS             complement(2567..3088)
                     /locus_tag="ACV5NJ_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HHY6486321.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            3287..6979
                     /gene="nifJ"
                     /locus_tag="ACV5NJ_002275"
     CDS             3287..6979
                     /gene="nifJ"
                     /locus_tag="ACV5NJ_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HHY6486322.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
BASE COUNT         2370 a         1190 c         1549 g         2006 t
ORIGIN      
        1 ggtaacgtgt tgtgaccgaa ttaaatgaat taatgcctat tttttatatt ccttagtgga
       61 caaaaatgga gacagtgaag ttaggagcat tgtatccttt gttgcgtagt tcaaaaaagt
      121 aagcgatttc tataatggtc agggtaaacg gtgagggctg tccacgtaaa tcctactatt
      181 gaaatcacaa tgatcggaca ttcaaaaggt ggtgctgaag cagcaaattc agtagctata
      241 aatacgaatg cgattatatt taatccagcg acagtaaatt tatctgctta tgaattagac
      301 tcaaacacat atacagcaaa catggtagct tatgttgtcg agggagatgc attaaatgtt
      361 gtttttggag gagtatacac ctattggaga attaatcatg ttagatagac aatatgatgc
      421 accttagtgg agaatgatat ctcgcatagg taactcagta aaaaatcaag gtatggaagc
      481 aattatctct gcattggaag atcgatatag ataggcggat atgaatagta atagggggaa
      541 taaaatggaa tacaatggat caatgagaat tgtacaaatt atattttgga ttacatcggg
      601 aattgtgatc ataggaggag tttttttgat gttgccaagc ttaatatttc ctttctttgc
      661 attaatctca cctaaaatac ccgaaccgga aatcacctat ggagaatttc catttaggat
      721 tgaatacgaa ctagatggac agattaacat aattgaagat actgtcatag ctgagtttaa
      781 cgggtgtgag ttcagcgcag gttctatgaa aagagaacga agatggagaa gtagattagc
      841 tagtggtaga gaagatctac cttttgggga cgatctagga atctatttta gtcgaggaag
      901 tgcgcaatat tatatggggg aaaatgtaca atcaatgtca ttaaaaccac atatagccct
      961 tagaaatctc gaggaaggtt ttaaaagaga ggtagacttt atattaggag aaagtggata
     1021 cataagaact gtacttaatt tcggagaagc gcaagaagtt ttgacacaat acggaattac
     1081 cttaatcaac tgggaaatat ctgaaccgat tgttaacaat tttggtgatt agaaaacaat
     1141 tacgataaat agaaacaagc caaatcatca gcaagtaaaa gctgatgatt tggcctttct
     1201 tatacgagta gtaggaattc atagcgaata ttattacgac cagtagatat tttgatatta
     1261 aaggagtatt acgaactaat atgtgagaat gtagaggtga tgtgcgaata atgagaaatt
     1321 tatcaaatag aaacaaaata ttaatcatta ttgttgttat agctgtgttt catctgggaa
     1381 cgaatgcggt tttaagtaga ataatattgg gtcctaagcc accaagacca gaaattaccc
     1441 gaggggagtt tgattttaga ttagaatatg aagtagatgg ggaaaggatt gtgatagaag
     1501 atacaattgt tgcattattt gatggatttt ctgcagatgc aggtagcatg gcatggtatc
     1561 gcacgtggcg tctacattta gcaagtgata gacgatcacg aaatatctta ttagatgaat
     1621 tagaggatgg tcgaagaata tattatgtcc cagaaagtgc taactattct atgggagatg
     1681 tacagaaaaa aagagagcct aatcctaatt ggtatccatt taatggcgta acaatagaat
     1741 atccacgcaa taaaacacct gaaataggtg caaaatttat aagtggttta gaagatctgt
     1801 acgataggtt tggaatccgt cttattagtt gggaacacga tccaccaata gaaaatcgct
     1861 ttgaataatc gaaatctact aatccatttc atcagccagt gaatgttgta atggaacaat
     1921 ttggtttagt gttcaaaacc attgaaatac taggctgtga tcagcaaaaa tgaatctgaa
     1981 taaaaaagag agcaactatt agctgagagg agttaatagt tgttagcttt atacgaatca
     2041 aagtggcaaa tacagatgcg tatgcatttg tgtttgccat cttgcgtttg gtagacgaga
     2101 taggtataag ctaaagaaac aacttgttta aaataaacga cctagctcaa caaatgacca
     2161 tctaatcaac taacagcata cataaaagca actatttttg aagtagaatt gaaatcggga
     2221 taacttaaaa agtgattggg atactagtga gaatcaccgt acataaatat tactcgttaa
     2281 gggaaattga gaaattttgt atgaaaattt aagaattagt tagtatgcgc aagtcaaaaa
     2341 tatgaataaa ttatttggtg gagataattt gcttagcttg tcataagaaa tgagataatc
     2401 tggcagatgc aagcttttgg acaccattta ttcggaataa ggaggctggg acagaagcgt
     2461 ttaactccaa gaaataagaa ggagttgctt ctgcttccgc cgtttatacg tgtttagagc
     2521 gtgagacaaa agtgttcttt acttttgtcc cacgctcctt atttttttat tttgtttgga
     2581 tcaattcttt taattcctct attgaaatag attcatcaaa gatttcttta cccaagatga
     2641 cagttggaac aaatcgaata tgtgcttgct cagcttcgtt gatgacagct tggctcagct
     2701 gttcgtcttt ttgttctgtc agctttagtt tatctactgc aaaatccgct acttcttgga
     2761 gagaaagatg tttccactcg tcttgagtat cgaatatttg tttgatctct ttgatcgctt
     2821 tttggccatc gctgattgtg agataccggt gcatcacgtt ccctcgctgt aagctttctt
     2881 tttctttatc gaacaatttg atcactcgct gcaattgccc gctttggact gcttcttcta
     2941 atagctcata agattcttca aaccattgtc ggcaatacgg acaagctaag ttgatgaatt
     3001 cgaccatttt tttaggagca ttcgatgaac cataaaggat tcctttttgt gtatttgttt
     3061 tcgttgcatc aatcactgat atatccatgt taaaacacct ctttcgctta tagtttatac
     3121 atgtctgaca aaaaaaggca acgatttaga ctgtttgtct aaaaattaga tgaccaattc
     3181 aaatagatac atgtaaaata tttttataaa taaaagaaaa aaatggcatg ttttacgtta
     3241 taatggaaca gataataaga gagaatatga acaaggatga tgaatcatgc gaaaaatgaa
     3301 aacaatggat ggcaatacgg cagccgcata tatttcttat gcatttaccg agattgctgc
     3361 aatatatcca ataacaccca gttcgaccat ggctgaatta gtagatcaat ggtcagcagc
     3421 aggaaaagaa aacgtattcg gtcaaccagt aaaagtagta gaaatgcaat ccgaagcagg
     3481 agcagctggt gtggtccatg gatcattgaa aactggtgca ctgacaacta catataccgc
     3541 ttcacaaggg ctgcttttga tgataccgaa tatgtacaag attgctgggg aactactgcc
     3601 aagtgttttc cacgttgcta gtcgggcagt gacgacaaat gcgttgaata ttttcggaga
     3661 tcacggagac gtgatggctg caagacaaac aggatttgct atgttagctg aaagcagtgt
     3721 acaagaagtg atggatttag cacctgttgc ccatcttgct tctattgaag gagaagtccc
     3781 ttttttgaat ttctttgatg gattccgtac gagtcacgag attcaaaaaa tcgaagtttt
     3841 ggaatatgaa gaattagctc ctctgatcaa tcaggagaaa ttagcttcat ttcgtaaacg
     3901 agggatgaat cccaatcatc cgactgttag tggtacgaac cagaatccag acatccattt
     3961 tcaacaacgg gaaacgatca acccctatta tgataaatta cctgaaatcg ttcaaaaata
     4021 tatgaaagag atcaaccgat tacgaggaac ttcttatgac ttggtgactt attacggagc
     4081 agacgatgcg gaagaagtca ttgtttctat gggatcagtt gcacagacga ttgaacaaac
     4141 gattgattac ttgaatgcaa acggacggaa aacaggtttt ttgaatatcc atctttatcg
     4201 tccttttcca gtggaaacat tcgtgcaaaa gttgcctcat acagtaaaaa caattgctgt
     4261 tcttgatcga agtaaagaac caggggcagg tggagaacct ctcttattag acgttcaaag
     4321 tgcgttatat gattctgaac ttcgcccggc tgttattggt ggtcgctacg gattggggtc
     4381 taaagatgtg actcccgacc aaatctcagc agtttttgat gaattgaaaa aagacccaag
     4441 catacgcaaa aaacggttta ccatagggat cgtggatgat gtgacttatc aatctttgga
     4501 accaaaggaa agtctggatt tgacagaacc gcaaacattc caggccaagt tttggggatt
     4561 tggatctgac ggaacagttg gagcaaataa atcagctatc aagattattg gagatcacac
     4621 cgataaatat gcgcaaggct atttttatta tgattcgaaa aaatctggcg gtctgactgt
     4681 ctctcatctt cgttttggag atacgccgat acgttcagcg tatcttgtcg agcatgcgga
     4741 ccttgttgcc tgtcacacac cagcttacct gcatagctat gacttggtca aaggattgaa
     4801 gccaggtggc atttttttat tgaatactct ttggagcgac gaacaattag aaacgcatct
     4861 gcccttaaaa ttaaaacgtt acttggctga aaataacatt agattctata cgatcaatgc
     4921 catgagattg gcccaagaag tcggtttggg acgccggatc aatacggcaa tggaaacggc
     4981 tttttttaaa ttagctgaca tcattccttt tgatgaggtg cttccgctgc taaaagaaga
     5041 agccctgaaa agctatggac ataaatcaat ggctatcgta gaaaaaaaca tacaagcaat
     5101 cgatcggacg accgaactgc ttcatcaagt cgcgatccca gaccattgga gaacgttgga
     5161 gatgcctgaa aaaatcaata aaaggaacac tggttatata caagaaatac ttgaaccagt
     5221 gaatgcacag gaaggaaatc aactctctgt tggtacactt gtagcaaatg gaatgacaac
     5281 tggcgaaatg cctttaggaa cggcttctat ggaaaagcgg ggcgtcgctt tagaagtacc
     5341 tgagtggatt agtgatcgct gtacgatgtg taatgaatgt gcctttgttt gtcctcatgc
     5401 tgccatccgt ccatttttag cagatgaaga ggaaatggaa gaagcgccag aaggatttat
     5461 cgtgagagaa atgcgaggcg cagacggcgt aaaataccgt atccaagttt cagtggaaga
     5521 ctgtacaggt tgtggtctgt gtgtagaggc ttgtcccgca aaagaaaaag ctttagtgat
     5581 gaagccttat gaagaacaga aagaacaagc tgtgaattgg gcttttgcaa tgacattaag
     5641 acaaaaagca aatccagtca aaggaaagaa tactgtcttg tctacacaat tcaatcaacc
     5701 gcttcttgaa ttctcgggcg cttgctcagg ctgtggcgaa acaccttatg taaaattatt
     5761 gacacaaatg ttcggtgatc gaatgatgat cgcgaatgcg acaggctgtt cttctatttg
     5821 gggcggtgct tctcctgctt ctccttatac gaccaatgaa tgtggtcaag ggcctgcatg
     5881 gtcaaattct ttattggaag ataatgctga atttggttat ggtatgcttc tagcggcaca
     5941 gactcgtcgc gaggctttgg caagttcaat caaagaagta ttgccaaaag tatcagaaga
     6001 actacgagaa ttgatggaag actggttagt acacatgttt gagagtgaag gaacgcagca
     6061 aagagcagca aaactgcaag ctgcgatcga aagcgaacga tcaattttcc ctgaactgga
     6121 ggatatatac aagagaaagg atctattcgt aaaaaacagc caatggatga ttggtggaga
     6181 cggatgggct tacgacattg gttttggcgg gatcgatcat gtacttgcca gcggagcaga
     6241 cgtgaatatc cttgttttgg ataacgaagt ctattccaat actggcggtc aaacctcaaa
     6301 agcaacacca gcttcggcaa tcgctaaatt ttcggctagt ggaaagtatg cttcaaagaa
     6361 agatctagga atgatggcga tgacatatgg aaatgtttat gtggcgcaaa ttgcttcagg
     6421 tgccaatcaa atgcaaacga tcaaggcatt tgaagaagca gaaaagtatc ctggtccttc
     6481 tctgattatt gcctatacgc cttgtatcac acatggcttg cttggcggaa tgaaagaatc
     6541 gcttaaagaa gcaaaagaag ctgtttcttc tggttattgg tcattatatc gatataaccc
     6601 tacaatggtc gaaaaaaaca agaacccaat gactttggac tacaaaaaac ctgatttctc
     6661 tgctatgcct gatttcatga ggaaacaagt tcgcttttcc tcgctagata acgcaaatcc
     6721 aaatcttgca aaagctttat ttgaaaagac tgtccacgac gctaaaacac gtttttacaa
     6781 ctatgctgca ctgacgggag aattagaaaa aatcaagaaa cgacttgaac cagaggaatc
     6841 tatcgatgta cccgttaaga ctagaaaaga aaaaacgacg gaccttgaag cagaagaaag
     6901 aagagctgct agaagagctg ccagagcaga acgacgtaaa aaaatggaac aagctacaaa
     6961 agaaaaaaag gaaaaataac agaaaaaata taatgaataa ttctctgaag tttaaaaata
     7021 tttattgcag agaaaaataa aacgcatact tgcaattttc ttgaaaatta tgcgtttttc
     7081 tcaagaaaaa tacgagcata tcagcctcta aaatt
//
LOCUS       DBKOIJ010000137         6953 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000137 DBKOIJ010000000
VERSION     DBKOIJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.140"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            180..1037
                     /locus_tag="ACV5NJ_002276"
     CDS             180..1037
                     /locus_tag="ACV5NJ_002276"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HHY6486323.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1030..1932
                     /locus_tag="ACV5NJ_002277"
     CDS             1030..1932
                     /locus_tag="ACV5NJ_002277"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HHY6486324.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1965..2315
                     /locus_tag="ACV5NJ_002278"
     CDS             1965..2315
                     /locus_tag="ACV5NJ_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HHY6486325.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2364..3287
                     /locus_tag="ACV5NJ_002279"
     CDS             2364..3287
                     /locus_tag="ACV5NJ_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HHY6486326.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3365..4258
                     /locus_tag="ACV5NJ_002280"
     CDS             3365..4258
                     /locus_tag="ACV5NJ_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHY6486327.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4296..4859
                     /gene="amaP"
                     /locus_tag="ACV5NJ_002281"
     CDS             4296..4859
                     /gene="amaP"
                     /locus_tag="ACV5NJ_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HHY6486328.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4873..5061
                     /locus_tag="ACV5NJ_002282"
     CDS             4873..5061
                     /locus_tag="ACV5NJ_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HHY6486329.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5074..5976
                     /locus_tag="ACV5NJ_002283"
     CDS             5074..5976
                     /locus_tag="ACV5NJ_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HHY6486330.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6008..6250
                     /locus_tag="ACV5NJ_002284"
     CDS             6008..6250
                     /locus_tag="ACV5NJ_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HHY6486331.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6506..6877)
                     /locus_tag="ACV5NJ_002285"
     CDS             complement(6506..6877)
                     /locus_tag="ACV5NJ_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486332.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2530 a         1185 c         1389 g         1849 t
ORIGIN      
        1 aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga tgcgttttgt
       61 gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt taaaaccaaa
      121 tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga gtgattttga
      181 tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat tatgctcctt
      241 acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga cgcataaaat
      301 tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa gaaacggtaa
      361 ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat atctacgaag
      421 acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct ttaccaaact
      481 tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg ttagtactag
      541 acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc gctattatcg
      601 atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac ttgtacttca
      661 attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt acagaaaaaa
      721 ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa gatgctttga
      781 atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg cagacttcgc
      841 tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa
      901 atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat acgttgaaag
      961 atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta acgaaagtag
     1021 gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac aaaattcgta
     1081 ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc taaatttgtt
     1141 cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt acgcttctca
     1201 gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa agaattcttt
     1261 acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat tgcgattccg
     1321 gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga catgatcgct
     1381 ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt agcagctgtt
     1441 gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt
     1501 tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga aaatattacg
     1561 caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca tgaccaagat
     1621 gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa
     1681 ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa
     1741 gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc gtggagcaag
     1801 gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac ggctttccgt
     1861 tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta
     1921 gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa attacaatgt
     1981 ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa atcgcactcg
     2041 cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca aaaggcagct
     2101 tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa atcgagcatc
     2161 cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa cgattgaaca
     2221 tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca gtactcgatt
     2281 ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga
     2341 ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa
     2401 aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag caagcaatcg
     2461 aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa gaagtttctc
     2521 ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta gtgattaatc
     2581 tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt gtactttccg
     2641 ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa
     2701 agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta ttagatattt
     2761 acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct ttggataaag
     2821 cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt gaaagagaca
     2881 tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa ttatggaatt
     2941 ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc catttgagac
     3001 agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt attggtgatc
     3061 tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta gattcagctg
     3121 cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat acaggaggat
     3181 taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct ttgatagcag
     3241 cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga atctatttaa
     3301 aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta ggaggaatac
     3361 agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg agaaatttcc
     3421 taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc ctgattgtgg
     3481 agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa tcactggtgg
     3541 agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg cagatatcgc
     3601 tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaagt
     3661 tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag cacctaaaga
     3721 aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac taaacgcagg
     3781 acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca aagacacatt
     3841 tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc atatcccagc
     3901 aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg aacatttatt
     3961 agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag caaaacagct
     4021 tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga caccattaca
     4081 actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa gtttactaga
     4141 acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat cgaataaagc
     4201 aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca acttataaga
     4261 agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg
     4321 gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa
     4381 cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact aattcatacg
     4441 tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt atcacgttac
     4501 ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat ggtgatgggg
     4561 agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg catgaagatg
     4621 aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg
     4681 taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca ttgatgaaag
     4741 aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa gtatcggtga
     4801 aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga gtagaatagg
     4861 gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat ctttggggta
     4921 cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac cttattgatc
     4981 ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta
     5041 gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa aaataataac
     5101 aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac accaaaccaa
     5161 tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca tccagatcca
     5221 gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga aaagcatgca
     5281 cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc aatcgatcct
     5341 tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca agaaaaactt
     5401 agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa taaccaagga
     5461 aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga agataaagtt
     5521 gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc tgcaaacggt
     5581 ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac ggctgggatc
     5641 gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt ggaatatggc
     5701 aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat
     5761 atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa
     5821 gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc
     5881 ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag cggcgcacaa
     5941 aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac attaggagga
     6001 acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag caattgcagg
     6061 agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag caggtttagt
     6121 tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg caggaatggc
     6181 aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat tctttgctcg
     6241 aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca tcaagtaata
     6301 agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat attatcgaag
     6361 atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac ttttgtcaca
     6421 gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc cttaaatgtg
     6481 tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat tttcttgaga
     6541 taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg atccacaaat
     6601 ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag agattgaaaa
     6661 agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa taatagctcg
     6721 taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca gcaatcgtat
     6781 catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga atatagatca
     6841 cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta attgtgtgag
     6901 ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt cgt
//
LOCUS       DBKOIJ010000138         6869 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000138 DBKOIJ010000000
VERSION     DBKOIJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.141"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..536
                     /locus_tag="ACV5NJ_002286"
     CDS             <1..536
                     /locus_tag="ACV5NJ_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HHY6486333.1"
                     /translation="GYNSDLISEYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELE
                     IEDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGN
                     GIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSK
                     RYQRGIKNLVKSYLKMN"
     gene            539..1435
                     /locus_tag="ACV5NJ_002287"
     CDS             539..1435
                     /locus_tag="ACV5NJ_002287"
                     /inference="COORDINATES: protein motif:HMM:NF024700.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHY6486334.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1485..1874
                     /locus_tag="ACV5NJ_002288"
     CDS             1485..1874
                     /locus_tag="ACV5NJ_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HHY6486335.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1861..3360
                     /locus_tag="ACV5NJ_002289"
                     /pseudo
     CDS             1861..3360
                     /locus_tag="ACV5NJ_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4108..5934
                     /gene="ltrA"
                     /locus_tag="ACV5NJ_002290"
     CDS             4108..5934
                     /gene="ltrA"
                     /locus_tag="ACV5NJ_002290"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HHY6486336.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            6047..6869
                     /locus_tag="ACV5NJ_002291"
                     /pseudo
     CDS             6047..6869
                     /locus_tag="ACV5NJ_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         2394 a         1116 c         1472 g         1887 t
ORIGIN      
        1 atggatataa ctctgatttg atttcagagt ataaaaatga gattgaaaca tgtgtaaacg
       61 aaataagaaa attaaattgg gatattgttt ctgtgaaaaa agaaatacaa gataaggcga
      121 aggtagagct tgaaattgaa gatttaaaaa aacaagtaga aaagctatct cataagagtt
      181 ataaagatgt ttatgatgag tattcaaaag agacagatat ttatagggaa ctgaaacgtg
      241 atgtggaggc acttaaggaa attcccaaaa atctaactga tagtgttgac aaaattgact
      301 ttggcaatgg tataaaagtt acagacgaaa ttgatgaaca ccgcggagaa ttgattaaga
      361 atttagtgac caatcgggct aagattcaaa caataattaa tgaaatgtca gatgaaattg
      421 atagttatag agcagagctg aataaatcag attggaaagt taactataat gaggtttcaa
      481 agaggtatca aagaggtatc aaaaacttag tgaagagtta tctcaagatg aattaaaaat
      541 gttacaaaac cttgattctt tatatcaatc tttatcaaaa actgaagagc gattggaaga
      601 aattattcaa caagaaataa agcttaaaga ttaccaagtt gaggttgact cagcaatgtc
      661 aagaatgaat aaatcatatc aaaatttcta ttctgagaga agtagttcct cgcgcgaaat
      721 tttttctgat attacaggaa tagaggctac tattcaacca aatagggatt tcaataatta
      781 tgttaaagag ctcagagctt gtattaataa ggaggacact tttgacagtg agtttggatc
      841 tcttattgat aagctgacaa aaaattccat tactaatcaa gatatatata atgacatact
      901 tgatataaga gttagtgggg aaagtagtat tttttcagat aaaaaattaa tcaattctat
      961 aagaaaattg aatgatgcac aagttcttga attacaaacc cttaagccaa atgatttagt
     1021 agttatccga cttgaactaa atggaaaaaa agttagttta tcgaatgcta gtgctggtca
     1081 aaagacatct gcaattcttt ctatgatttt ggcgtatgga aattcaccac tcatattgga
     1141 tcagcctgaa gatgatttgg atagtcaatt aattaataac ttaatcgtaa gaagtatcat
     1201 tacaaaaaag gaaaacagac aaattttaat tgtaactcat aatgcaaata tcccagttaa
     1261 tggtgattca gaatggttga ttagcatggg agattcaaaa gaaatatcgg ctgatcagtg
     1321 cgggtcggta gatgaaccca atatcaagcc aagaatatgt actgtgatgg aaggtggcga
     1381 ggatgctttt acaaatagag cgaaacgcta tggttttaaa caaattgtag aataacacta
     1441 gagggtgaga catattatta tgtccgccct ttttaggagg aaaaatgaaa aaaataaaaa
     1501 gctataccag tatctgggca gttgaaaaag tcatttacgc aatcaatgat tttcagttgc
     1561 cttttcctgt gacttttaac caaatggctt ggttcgtttt atctttactg tttgtcatcg
     1621 tatttgccca tgtgcctcca ctatctatga tagaggaggt ctttttaaaa tacttaggca
     1681 taccggtggc tgtcacttgg tttatgtcgc aaaagacgtt tgatggcaag aaaccactgg
     1741 gatttttacg atcctttatc agctatcact tgcgatttaa ggtgactttt gcaggaaaga
     1801 aagtcaaaga acagaagaaa cgatgggatg aacccagtac tcttgtaagg agtgtgaact
     1861 atgtatccga ttaagtatat tgaaaataat ctcgtcttta accaagaagg cgagtgtttt
     1921 gcctactatg aattagtgcc ttacaattac tcctttttgt cacccgaaca gaaatttcaa
     1981 gtgcatgata actttcgcca actaattgcg caaaatcgtg aaggcaagat ccatgcgtta
     2041 caaattgcca cagaaagtag tattcgcgcc acacaagaac gatcaaaaaa agaaattacc
     2101 ggtagactca aagaagtagc gaaacaacga attgatatac agacagatgc attagtatcc
     2161 atgattggcg atagtcagat tgattatcgc ttttttattg gctttaaact aattgcgaca
     2221 gatgaagaag tcaatctaaa gagtctcaag aaatctttct tttctgggct tcaagaattt
     2281 gtctatggag tgaatcatca tttgatgggc gattttgttt ccttatccaa tgaagaaatt
     2341 cgctgctatt ctaagcttga aaaattaatg gaaagcaaat tggctcgaag atttaaagtc
     2401 aggcgagtca caccgagtga cctgacgtat ttgattgaac atatttatgg cgaaaaaggg
     2461 ataccgtttg aagaatacga atttcagcta ccaaagaaaa agctaaaatc agaaacactg
     2521 gtgaaacgtt atgacttatt acgtccaaat cgttgtttaa ttgaagaaaa accaaggtgc
     2581 ttacggatgg aacatgagaa tcatgagtcg tacgtagcgt atttaacaat taatacaatt
     2641 gtgggggaga tggaatttcc ttcctcagaa cttttctatt accagcaaca acaatttaca
     2701 ttcccaatcg acacaagtat gaatgtagag attgtgacga ataaaaaggc tttagctacc
     2761 gttcgtaata agaaaaaaga actgaaagat ttagacaatc atgcttacca atctgacaat
     2821 gaaacgaatt ccaatgtctt agatgctttg gattccgtgg atgaattgga aaccaccttg
     2881 gatcagtcaa aagaatctat gtacaaactg agttatgtgg ttcgagtgag tgccgaatca
     2941 gtggatgaac taaagcgacg ctgtgatgaa gtgctcgatt tttatgatga taccaatgta
     3001 aaactagtcc ggccctttgg cgatatgatg gggctacatg aagaattctt acccttaagt
     3061 aaacgatata tgaacgacta tattcagtat gttacgtctg attttttggc ggggcttggt
     3121 tttggggcta ctcagatgct aggagaatta gaagggattt actttggcta caatgtggat
     3181 acaggtcgta atgtttacct gaaaccggca ttggcttcac aaggagtcaa gggttcagta
     3241 accaatgcgc tggccgccgc tttcctgggt tctcttggtg gaggtaaatc ctttagtaac
     3301 aatctcttag tctactatgc tgttttgttc ggtggtcaag ctgtgattgt cgatccaaaa
     3361 gggggtgcga aacgttccta cttgaaaaga gtaggcacag cacttcatta aatcaatatt
     3421 taatgtgtgt tggagaacta aacacacgaa tagtcgaatg acggggtaac gccctgaaag
     3481 gctactttaa tactctgact ggcagaaact tgagtaaaca aggctctgtc agaagctcgg
     3541 tgaagtcggc agacagacac cgtaacagat aatgctgacg aaagattccc agaggtgggc
     3601 gttgtgtcct atatgccgga agtctataaa atatctatgg ttagaatgta gatgagaggt
     3661 ctactgacga atccttgaat gtacaggtct atatcggggc tggatagaaa tgtccagtga
     3721 cacaaacagt gtcgctactg tgggtgagta agactccgca tttatgaaaa tccatataac
     3781 gttaaaggcg ttatcaaggt agcaggctta tagaggaaac ctaaggatat atgtacagat
     3841 agtgtgtttg gaacgttgaa agctgacaac atggagattt tactgtctgt gaagtgttca
     3901 catcagaaag ttattgtgag attagcaatg atataaggtg tgttaatgtc agtgagagtg
     3961 gtggcacagt acctatgaaa ccgtgataac aagcggtgga gggatagcca ctagtcatat
     4021 gaagaataga attactaaga aaaatggtat gggttcgagt aagactaaga gatgtacttg
     4081 ctcctgcaaa caaggagggt acagaccatg ctaaagaaaa acaagctccg ctataacgag
     4141 tattatgata tgcaacatat ttatgacgag ttatacgcac agagcaagaa cggtaataac
     4201 ttttataagt tacttgaaat tattggttcg gaacaaaata tttgtttagc ttaccgtaat
     4261 ctgaaatcaa acagtggcag taaaacggct ggaacggatg gaatgacgat agacgatata
     4321 aaacaactca gcaatgctga gattgttgct accgttcggg aaagccttag caactaccgc
     4381 cccaaatcag tcagacgtgt tttcattcca aaagctggga gcgataaaat gcgtccattg
     4441 gggattccgt gtatctggga caggctggta caacaatgta tcctgcaagt cctagagccg
     4501 atatgcgaac caaagttcca taatcattcc tatggattcc gtgcgaatcg aagtgcccac
     4561 catgcagtca gcagagtaac gacactgata aatttatcaa aatatcatta ctgtgttgac
     4621 gtagacatta aaggattttt cgataatgtc aatcatggaa aactattaaa acaaatctgg
     4681 acacttggta ttcgggataa aagattgatt tgcattatca gcaaaatgct gaaagccgaa
     4741 attgacggcg agggtgtccc agaaaaagga acgccacaag gcggtctgtt aagtccactg
     4801 ctctccctca tagtccttaa tgaattagac tggtgggtaa gcagtcagtg ggaaacattc
     4861 cagccgaaga accgcagtaa aaatgggtgg cttcaatacg caaagaaata taccaaactg
     4921 aaaagcggtt ttattgtacg ttacgctgat gattttaaaa tcatgtgttc aacctatgga
     4981 gaagcacaaa gattttacca cagtaccgta gattttctta ataaaaggct gaaattagaa
     5041 atcagtccag agaaatcaaa ggtagtaaat ctgaagaaaa attcatcaga ttttctgggg
     5101 ttcaaaatca aagtcattcc aaaaggaaga acgaaacacg gttatgttgc caaaacagat
     5161 atgaaccaaa aagcattaaa gaaagcgaaa acaaatctaa agttaaaggt aaaagatata
     5221 gccagacata cgactggctt caatatcagc agatataatc tgacagttat cggtatgcag
     5281 aattactact gcatagcaac taacgtatat aacaacctga cagaagtaag ttacgctctc
     5341 ttaccgacca tacgaatccg tttaaggaac attgcaaaat cagttccatt tgaaagtaca
     5401 agttcagaat ttcagagcag gacaaaagga atcagaccaa aaacaaaaat agtgatgatt
     5461 gcggataatc cgctattgcc gattcaagga gtacaacaca aaaatcctat gaatttctcg
     5521 caagatattt gtaattttac aaagcaaggg agaaacaagg tgcatgagga cgttgtagtt
     5581 gtaacaaaag aagaaatccg agcgttgttg gaaaatgaaa accctgccga tagcgtagag
     5641 tttaacgaca atcgtatttc ggcatatatc gcacaacaag gcaactgcta tgtaatgaat
     5701 cgtcgaggaa ctccgtccac tctgatatgt attcacaaat cagacaaagg agataacctt
     5761 gacagatatt ctaatctggc atttgtggaa attcccattg caaaggcaat cctaacagaa
     5821 tcagcagacg aagcaaaatc actgttaaaa ggttatgttc ttaatagtca gcagaagaaa
     5881 aaactgaaca gaatacggac gaattacgga taccagacaa taactgggaa ataacttata
     5941 aatctatttg gatatgatgg aacgccgtat gaagcgaaag tttcatgtac ggtgtgaagc
     6001 gggggaaaac ttggagataa catcaaaaag ttacctatcg ctatagcgag gagggtggaa
     6061 agaaaccttg ccagaaattg cggatgaaat cagtatcctc aacttgacga gtaagtcaga
     6121 aaatcaaggt ctgcttgatc cttatgtgat tatgaagaaa aagaaagact cggaaagttt
     6181 agcaattgat attctgactt ttttaacagg aatttcaagt cgagacgggg agaagtttcc
     6241 tgtgttacgt aaagccattc gaacggtgac tcaaagtgat caacgggggt tattacaagt
     6301 tattaacgaa ttgcgaaaag acgggtcacc aatcgcggaa aatatcgccg atcatatcga
     6361 gtcctttacc gattatgatt ttgctcattt gcttttctca gacggctcga ttacccaatc
     6421 gattagttta gataaacagc taaatatcat tcaagtggcg gatttggttt taccggatgc
     6481 agaaacgact tttgaagaat ataccaccat ggaattgctt agtgtttcga tgctaatcgt
     6541 catttccact tttgccttgg actttatcca tagtaaccga gaaatcttca aaattgtcga
     6601 tttggatgag gcttggagtt ttcttcaagt ggctcaagga aaaacgttat cgaataagct
     6661 tgttcgtgct ggtcgggcca tgaatgctgg ggtctatttc gtcactcaaa atagcgacga
     6721 tttattagat gaacgtttaa aaaataatat cgggttgaag tttgcttttc gcagtaccga
     6781 tattcatgaa atcaagaaaa ccttggaatt ctttggtttg gatcaggagg atcaaagcaa
     6841 tcaaaaacgc ctgcgagatt tggaaaacg
//
LOCUS       DBKOIJ010000139         6814 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000139 DBKOIJ010000000
VERSION     DBKOIJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.142"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..182)
                     /locus_tag="ACV5NJ_002292"
     CDS             complement(<1..182)
                     /locus_tag="ACV5NJ_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HHY6486337.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACV5NJ_002293"
     CDS             517..951
                     /locus_tag="ACV5NJ_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HHY6486338.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACV5NJ_002294"
     CDS             974..1294
                     /locus_tag="ACV5NJ_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486339.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACV5NJ_002295"
     CDS             1435..1626
                     /locus_tag="ACV5NJ_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486340.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACV5NJ_002296"
     CDS             complement(1697..1954)
                     /locus_tag="ACV5NJ_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HHY6486341.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTHSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACV5NJ_002297"
     CDS             2112..2375
                     /locus_tag="ACV5NJ_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486342.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACV5NJ_002298"
     CDS             2399..2587
                     /locus_tag="ACV5NJ_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486343.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACV5NJ_002299"
     CDS             complement(2670..2855)
                     /locus_tag="ACV5NJ_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486344.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACV5NJ_002300"
     CDS             3070..3441
                     /locus_tag="ACV5NJ_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HHY6486345.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACV5NJ_002301"
     tRNA            complement(3591..3663)
                     /locus_tag="ACV5NJ_002301"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACV5NJ_002302"
     CDS             complement(3709..4137)
                     /locus_tag="ACV5NJ_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486346.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACV5NJ_002303"
     CDS             complement(4197..4955)
                     /locus_tag="ACV5NJ_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HHY6486347.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACV5NJ_002304"
     CDS             5250..5447
                     /locus_tag="ACV5NJ_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486348.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACV5NJ_002305"
     CDS             5553..6035
                     /locus_tag="ACV5NJ_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486349.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACV5NJ_002306"
     CDS             complement(6093..6689)
                     /locus_tag="ACV5NJ_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HHY6486350.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2311 a         1053 c         1341 g         2109 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga atgcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaa
//
LOCUS       DBKOIJ010000140         6731 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000140 DBKOIJ010000000
VERSION     DBKOIJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.143"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            14..904
                     /locus_tag="ACV5NJ_002307"
     CDS             14..904
                     /locus_tag="ACV5NJ_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HHY6486351.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(977..1801)
                     /gene="nadE"
                     /locus_tag="ACV5NJ_002308"
     CDS             complement(977..1801)
                     /gene="nadE"
                     /locus_tag="ACV5NJ_002308"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HHY6486352.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1813..3285)
                     /locus_tag="ACV5NJ_002309"
     CDS             complement(1813..3285)
                     /locus_tag="ACV5NJ_002309"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HHY6486353.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3922..4284)
                     /locus_tag="ACV5NJ_002310"
     CDS             complement(3922..4284)
                     /locus_tag="ACV5NJ_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HHY6486354.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4507..5259
                     /locus_tag="ACV5NJ_002311"
     CDS             4507..5259
                     /locus_tag="ACV5NJ_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHY6486355.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5399..6322)
                     /locus_tag="ACV5NJ_002312"
     CDS             complement(5399..6322)
                     /locus_tag="ACV5NJ_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6486356.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6337..6609)
                     /locus_tag="ACV5NJ_002313"
     CDS             complement(6337..6609)
                     /locus_tag="ACV5NJ_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486357.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2052 a         1347 c         1154 g         2178 t
ORIGIN      
        1 agaggtgata tatatgcaac tacaaataaa cgaagaatcc ttaccggtat atgaagcatt
       61 agctagtaaa acaaggatca agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa
      121 agatttggca aaagaacttg gagtgagcag tgctatcacg acgatgcacg tgaaaaagct
      181 agaggaagca aacatcatca aaacagaaaa agtaggacaa caaaaaattt cgagccttcg
      241 tgtcgataaa atcgatatta gttttccaga aaaaatattc aatgcttttg atacgaaaga
      301 aacatcgata cctattggtc actatacaaa ttatgcaatt gaaccaacat gcggtttagc
      361 tacgatccat gattttatcg ggaaagtgga tgaaccacgc tattttatgg atcctcgaag
      421 gatggatgcc cgcattctct ggtttaccag cggatttgtc gagtatcagg cacctaattt
      481 tttaaataca gaggatacat tggagatgtt ggaagtttct gtggaaattt cttctgaatt
      541 tcctttttca aatgacaatt ggccttcaga tatcactttc agcctaaatg gtgtggagct
      601 aggaacatgg acgagccctg gagattttgc tgatatccgc ggtaaatata cgcctgattg
      661 gtatccagat aatcttaatc aatacggctt gctgaagacg atccgaatta ccaaacactt
      721 gacgaatatg aatggagaac ccttgtcaaa catcacgatc aacgacatcc ctaaagagca
      781 agacacttgg catttgcgaa tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac
      841 tttatttggt aaaggattcg gtaattacga tcaggatatc aaattaaaag tttactacag
      901 ctgatatttt tcttttctat tttataaaac aaaaacagcc aatccctatg aagggacggc
      961 tgtttttgct ttattgttat ttccagaaat catccaaaac ggaaattggc aaatgtcgtt
     1021 tatgttgtgt cttgttccac catgcttcga ttttttcttg tgcttgagcg ggaacttctt
     1081 ttccttctag ataatcatca atttcattat atgtgacacc tagtgcgact tcatctgcaa
     1141 tcattggttt cccatcttca agatctgcag tcggaatttt tgtgtataat ttttccggtg
     1201 cattcaatgc ttgaagcaat tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa
     1261 tgtcggctcc accatcacca aatttcgtga agaatccagt aatgttttct gcagcatgat
     1321 ctgtccctaa tacggctcct gctctttctc cagctacggc atattgcgta atcatacgtt
     1381 gacgtgcttt gatatttcct ttgttaaaat cgctgattcc cacacctgct tcgcttaata
     1441 cttgtacttg tgcatcaaca gctggtttga tatttactcg cagactaaca tcgggctgga
     1501 taaagtcaag agcagctttt gcatcttctt catctgcttg ttcaccataa ggaagacgaa
     1561 cagcgataaa ttgatagttt tcatcttttg tttcttcacg catttcttcc atcgttagtt
     1621 gcgccaaacg gcctgctaaa gtagaatctt ggccgccgct gatacctagt acaaaagttt
     1681 taagaaacgg atattttttc aaatatgctt tcataaagtc aatactttta cgtatttctt
     1741 cttttggatc aattgttggt ttgacaccta attcattgat aatttctgtc tgcaggctca
     1801 tttattctcc tcctatgctt ctgtatgaag ttctgctacg ctttttttca ttcgttccat
     1861 gatagccatt ttatggttcc agcattcagt agacagatcg actgggtatt tttgcggatt
     1921 caagtctcgt ttatactctt cccatagtga atctagattt tcttttgtat attctttgat
     1981 ttcgtttagt gcaggtaatt cgtaaactct ttttccttcg ataaagatat cttgtaaaac
     2041 tgggcgagct gtaaagtctc gaacagtttt attgataaac gtatgcactg gatggaacat
     2101 gaagatagct tcttcttctc gagggtcttc atcccataat gtcacgtagt ctccttcaga
     2161 tttcccatca aaatttcgag tgatgcgcca cacttgtttt ttccctggag tagttacttt
     2221 ttctgcattg cttgataact tgatcgtatc catcattttt ccttcatcat cttcgatcga
     2281 tactagcttg aatactgcac caagggctgg ctgatcatag gctgtgatca attttgtccc
     2341 aacaccccat acatcaattt tagctttttg catctttaaa ttcaaaatcg ttgcttcatc
     2401 caagtcattc gaagcataga tttttgcttc cgtaaatccg gctgcatcta attgttcccg
     2461 aacacgctta gatatatagg ccatatctcc actgtcgatg cgtacgcctt ggaagttgat
     2521 tttgtcacct aattctttcg ccacgcgaat cgcacttggc acacctgatt tcaacgtatc
     2581 ataagtgtcg actaagaaca cgcagtcttt atgggtttta gcgtaggcca taaatgcttc
     2641 ataatcattt ccataagatt ggactaacga atgagcatgg gtcccgctag ctggtatccc
     2701 aaatatcttc ccagcacgta cattgcttgt tgcatctgct ccgccgatat atgccgctct
     2761 agttccccag actgctgcat ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg
     2821 atcttctccg atgacagatt tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt
     2881 gaccatattc aacagtgctg tttctactaa ttggcagtgc gctaaaggcc cttccacttg
     2941 gatcaatggt tcatttgcaa aaactagttc tccttcacga gcagaacgaa ctgttgcttt
     3001 aaattcaaaa ttttgtaaat actccaaaaa accttcagga tagacttcta atccacgcaa
     3061 gtaagcaata tcggaatcat tgaacgtcag attctctaaa taattgacta accgttccaa
     3121 tcctgcaaaa accgcgtatc cgtggttaaa aggcatttct cgaaagtaac attcaaaaac
     3181 agcatgtaaa tcagctcgac ctaattccca gtatgtctgc atcatattga tttgatatag
     3241 atccgtatgc agcgtcaaac tgtcgtctgg gtaagtatta ttcatcttgt attactcctc
     3301 ttggcaagtt ttcatttcct acccattata acaaaaaaaa ttaataggaa aagaaatctt
     3361 ccattttttc tgaaaaagca catgttttac ggatatttct tgctaaaaat gaactccatc
     3421 tgctaaaact atcctttctt attggtattt aacctaaacg agaaagaata cttattttat
     3481 tcaatgtatc cgagacacga agaaattagc gttttctatt tttcttactt gataaaataa
     3541 gtagattttc ctactacaat cgtagccgtt aagataccga tgattgaaca aatataacca
     3601 aatgtctcta caacataatc aatggcaaaa gtttttgaag caataatacg tctttccttt
     3661 tttgcttctt atccccaaag aaacggatat aacttattat gtattgaatt tttttggaaa
     3721 ggcgaattat gacaagatat accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt
     3781 ttttaaaaag atacaaagta agaaagcagc tgtcaataga atggaaacaa aaatggcgag
     3841 acatcaatca gtctcgcctt gttttttatt gtatacatta gatatggagc aggttaccat
     3901 aaagatgatt attagtcgtt attaaataga atccttcgta tttttttgtt ctatcactac
     3961 aatatcttta tttggacgtt tgaattgtat tccggcacgt tctagctcct gtttcactcc
     4021 tacatggact ttgacatctt ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat
     4081 ttcttgcctt gtgatttttt tcttcgtatt gatcatcacg acttgctttt tatcatatgt
     4141 gtctaccaat agcagttgtg ttcgatctaa cgaaaataat ttgtaataat taatagccgt
     4201 attcccaaaa aagaattcca ctactttagg ataaaatgtc ttaatattta aattactatt
     4261 cacaggtgtt tcgattagct tcatctcatt cctcttttct atatagtcga tttagatcca
     4321 atcttaagtt atgttaccat ctttcctagc attaaggaat cggttacaat tgaaccttta
     4381 aagacaaaag gatttacctt ttatcactta atagtataac ataatttcat gagaaaaaaa
     4441 tacaattttt tcttgaccat ctcgttacgt aaaggattat gcttacattt gtcaggagga
     4501 aaaataatgg agtacacaat aaagaaaatg tcagagatat caggagtcag tccgagaacc
     4561 ttgcgctttt atgatgagat tggactgcta aaaccagcta ggatcaattc ttcaggttat
     4621 cggatttatg gcaaaaaaga agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg
     4681 gcattcaagt tggatgatat ccaagaggtc ttagacaatc catcttttga ccaccagaaa
     4741 gctttaatca agcatcagca aatgttgctg gaaaaacggg cacaaatcga taccctcttg
     4801 acaactgtcc aacagacact tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa
     4861 aaatttgaag ggtttaaaca acaaaagttg aaagaaaatg aagaatccta tggacaagag
     4921 attcgacaaa aatacggaaa aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg
     4981 gataaagaaa cgtatgaaga aatgcaaact gttgaaaagc agatgttatt tgatctcact
     5041 ctttacttaa aagaaccaga agatgaaagc ttagcagacc gtatcttcca agctcataag
     5101 aagtggttat cttacagctg gcctgaatat cagccaaaag cgcataaagg actaggaatg
     5161 atgtacgaag ccgatgaacg gttcactgca tattatgacg agcgaagtgg tcatggtgca
     5221 gcaaaagctt taaatgaaat tatccaaaaa aaggcgtaat cttttacatg atccaacaaa
     5281 aaagtacacg taaaatgacc taataacaaa acagcagaca cccaagttag atttttatgc
     5341 taacttttgg tgtctgctcc attctttcac aagttttttc atcctacttt aaatatattc
     5401 atcgttgaat aaacgtataa ggtattttca ctttatcctt tttttctgta agaaacaatt
     5461 gaaatgccat ttctccgcaa gcttccagat ggtggtcgat ggttgggaaa ccaagaagct
     5521 cgctgatcag caaattatcg cgtccaatca ccactggtgt cttatcttgc agatagcttt
     5581 gcaagatgcc tgctgcaacc atatccgaat ttgtaaagac accatcaact ccaagctctt
     5641 taaaaaagaa gcctgcttgt tttcctttct ctgcatctat acaatcccaa aaaacaagtg
     5701 attcatcaaa agatggaaaa agttgatgac atagctgcaa agtgattttt gagttataac
     5761 ttagcctgcc acttcgcccc aacgtgatgc ccaattgctt tataccttgc tgcttcaaat
     5821 aagtaagacc ttctgtaatt gattgttctc gatcaataaa gacacagctt gcctgttttc
     5881 ccttgatttc ttcacaaaaa actagaggtc catatttttg atatgccaat aaatcatcta
     5941 gtgtattggc acgagacgta atgatcaatc catcgaaagc tttagctgca aattcttcaa
     6001 gataccttcg ctctttttct ttctgatagc ccgtcggtaa aagtgtcact ttgtaatcat
     6061 aatcaaacgc tgcttcaatg atcccactga tcaactgatc aaaatacgga tgattggtat
     6121 aaggcagaat cactccaatg cttttcgttt ttccataact taactgtctg gcatttgcat
     6181 tagggatata atcgacttct ttcatgaccg caagtatttc tttccttttt tcctcatcta
     6241 catatggatg tccattgatt acacgtgaaa tagtagaaac tgaataacct gtgagctttg
     6301 cgatatctcg aatgtttgcc actgctgctt cgccacctat acaaaaagat tttcttgtaa
     6361 atagtctacc acagctggac cgcattcaaa aaactgatac atttttcctt catgcgaaaa
     6421 actgatccgc tgcccgcccc aatatatatc ttttgtcaaa ttttcgattt cattcattgg
     6481 tatttctttt actatattcc tattcaaccc ttgaaaactg tacaacgtta atacggatcc
     6541 cttgaactcg acaaacgcat aaaaataatt gttcgttgtg ccatctgtta tctctacata
     6601 agtattcatt gcaagccctc cttttgatac tttgagtata gtgtatgaaa cgcgtctcat
     6661 agcaaggagt ttgttttgcc aaagaaaagt tttattcctc tttcattatt accaaagtct
     6721 cttattttaa a
//
LOCUS       DBKOIJ010000141         6658 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000141 DBKOIJ010000000
VERSION     DBKOIJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.144"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            52..1062
                     /locus_tag="ACV5NJ_002314"
     CDS             52..1062
                     /locus_tag="ACV5NJ_002314"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHY6486358.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1131..2075)
                     /locus_tag="ACV5NJ_002315"
     CDS             complement(1131..2075)
                     /locus_tag="ACV5NJ_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HHY6486359.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2059..4779)
                     /locus_tag="ACV5NJ_002316"
     CDS             complement(2059..4779)
                     /locus_tag="ACV5NJ_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHY6486360.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4776..5996)
                     /locus_tag="ACV5NJ_002317"
     CDS             complement(4776..5996)
                     /locus_tag="ACV5NJ_002317"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HHY6486361.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6277..>6658)
                     /locus_tag="ACV5NJ_002318"
     CDS             complement(6277..>6658)
                     /locus_tag="ACV5NJ_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HHY6486362.1"
                     /translation="IEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNI
                     EIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMK
                     KADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         1959 a         1272 c         1085 g         2342 t
ORIGIN      
        1 ggtatagtta atatataaaa ataataacta attacaggag tgcaaatttt aatgaaaaaa
       61 aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc agcttgttca
      121 ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt atctgatttc
      181 tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat gatcatttat
      241 aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga tgcagaatac
      301 gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc tgctggatat
      361 acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc tggcttgaaa
      421 gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt ccatccagaa
      481 gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt gaaaaaatca
      541 gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga tacagagaca
      601 aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc tgcagaagta
      661 aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac tacaacaaat
      721 cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc
      781 aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac taaactaagt
      841 gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc
      901 aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc aagtacgaaa
      961 gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac tgattcaaca
     1021 aaagaatctt caactgaaga aacaactgat tcatcaaaat aaaagaaaac aaaaaaagcg
     1081 aagacttatg tctcgctttt tttgttttga atgaaaaaaa taaatttttg ctataaatct
     1141 tttggtacat agaaacttcg gttgtccata ccgaaaatcc gatcggtgta ttttcctggt
     1201 tctgtttgac gaatcgaagt caacatcatc tgcatcgaag catcgatatt gtcgatttga
     1261 tgtaatattt ctgcttccat tattcttgga cgtacaggtg agccatattc taataagcca
     1321 tggtgtgcta aaaccatatg acgtaatacg agaacatctt cgtcagcttc gctgattttc
     1381 aacgcaagac aggctttagt tatttcttcg tcaatcaata ccagatgacc gatcaaattt
     1441 cctgcaagcg tatattctgt cgacatagga ccggaaagtt caatcacctt tcctaaatca
     1501 tgcaaaatga taccagcata caacaatgct cgattcagct caggatattc atcggcgatt
     1561 gatttcccta agtgaagcat tgtcaaagta tgataagcta atccactggc aaatgcatga
     1621 tggtttcttt ttgctgccgg aaactcaaaa aattgttttt gatatttatt aagtagatag
     1681 cgaacaatac gattccaatg cgcattggtg atctcaaaca acaattgatt gatttcttct
     1741 tccatttctt ctttcttcaa aggggctctt tccatgtata aactaggctc tcctggctca
     1801 tctacccttg ttaagcgcat atgaagaatc tttacttgag ggtttccttg ataaacctct
     1861 cttttaccgt ttagaaaaac gactgtgccg gctgtaaatt tattgatttc ctcctccgaa
     1921 gcatcccagt atttaccatc gatagtacct gaagtatctt ggaaagtaaa agcaataaat
     1981 ttttttccgt ttttagcgat tcttacatct gcacttttga tcaggacaaa ttgttcaaac
     2041 tgctcatcga ttactaaatc acgaattttt ttcattattt gctccttcta attgttttac
     2101 gatttggttc atctcatggt aatagcttac catttcttta tcagaagaaa aacaaattat
     2161 ttgatacttt tctccaaatt tagctaaaag ttcagctaat tgatactttc gctgatgatc
     2221 ataatgaagc cagccatcat caatgatgat cgggcatatc gaacggttag cttctaatga
     2281 gaggtaagca aatctcatcg ccataatcag ctgatctttc gtacctgtag ataactcata
     2341 gatgggaaat tctgtatatc cgtctgttaa agtcagtgta tcatttttca gtaatatctt
     2401 ctgataattt ccttttgtta aaatacctaa ataatcacct gctgctctta acaactgggg
     2461 taattgtctt tcactcatct ctgttgaaag atcacctaag aatgtcccgg cgatttcaag
     2521 tgctccccat tcctccagta attcctgtaa ttttgctttt tgccggctag cttgttgata
     2581 aagttcgtcc aatgtgccgt ctctttgcaa ctcttctact tgaagttgga gtcgttgccg
     2641 attttcactt gcttcttttt cttcttgctg gagttgggat tgcttttctt tcagttggcg
     2701 cagtcttaca gataatttct cttttgtcag ctcttcagga aatagtggag taagcatttc
     2761 cgataattca ttgtatcttt tcatctgctg ttcggcttgt ttggcttttt gtaaaaagac
     2821 aggtacttcc gaaggataag ataagcctgc ttgagtcaat tgttcgctgt attcttgtat
     2881 ccatactttt tgtttctttt tcaattgatt gatttcttgc ttcaataaag tattttgctg
     2941 atagcttctg gcaaatttca tctgttccat ctcttgtata aaacgggaaa gcaataccat
     3001 tttttctgaa attgtcttat cttgtatcgg taaccattct ttaacaaact caaatttttg
     3061 gtcgatttgt cgtagttttt cttgtatttc ctgttctctt ttatgtttcc gctcaaatac
     3121 gccatataat ttctgataat ctatgacttc ctgtccatat tctttcatga gtttgagcgt
     3181 atccatcttt cctaagtgcc gatgatccgc aaaacgatat acttcttgct gaagaatctg
     3241 tttttcttct ccagttctct tttgtttttc ttctgcttca gctaattgag ctaaggctag
     3301 atcaagttgt cctagttttt cttgccacat tccttttatc tcagagaaat catttttagt
     3361 tttaattttt ggttgccttt tgtagaagaa atagccaatc atgcctgcac caattagtaa
     3421 aaggatcact ttcaagggca ttggtacaaa tatacctgca atgattattg ctccgccagc
     3481 catcagcata tacggtttag atgaagagac tggtgctatc tctgactgat tttgtaataa
     3541 tgctggatag gtctcttcca agtcattcac ttctgcttca atcatctctt gctgttcttt
     3601 agctatttgg agacgcacag aaatctggct ttctagatca gcaatccttc tttcttcagc
     3661 cagaagatgc ttccattctt cttcatataa aaattcttcc ggtaacggat ttttctgcca
     3721 atgccacctt tgttccaaaa tcttcaattc tgaatttgtt tctctagtct caatttgtaa
     3781 tctttgccat tcttcgttta attgttccgc tgtaaattga agttgctgga gatcttttag
     3841 ttcctgctcc tgctctaaat aaaaataata tctggctgac tgctgatcca tcccgctatg
     3901 cttttccagt gttgcttcta attcttccaa acgctcattt gtttgctgat accgtttgga
     3961 gaaagactcc agatctaaaa gaagttgctc atcaggaaga cctgtagtgc tgtttttttt
     4021 gagagattgc cattcttcat ataaagaaaa attaagttct tgctgtctgg ctaatgaaaa
     4081 ttgtttgttc gcctctttta attgtgccaa taactgctgc tgttgcgact cgtattcctg
     4141 ttcttgttgg atcaacttgc caaaataagc ttcctgttct tgtttttgat gaattttttc
     4201 ttgcagctct ttccattcat ttaatttttg attgattagt aatttttggc cttttttctt
     4261 gaataattgt tgtgctttct gataatattc ttgccgcttt tggaatactt gagaactgcc
     4321 cgtgatgcct aaagaaatca gcgcatcatg tagctctttt tctctcaaat gatctagttc
     4381 catcaactgc tcttgctgga aagtaaatac ctgctggaat aactctttcg tcaaaggtgc
     4441 gatcagtttt tctagtaact catcacttcc cacttgatcc cccagccaca ccttcgattt
     4501 tccgcgattt tgctgcttgt agcgctcgac agcaaattca ccatatacag gatgtttaag
     4561 ccatattttg ccgccatagg ctgagccgtc ttttggtgta taatcctttt ttttacggcc
     4621 ttttgaaggg aaaccaaata atattgcttg aataaattga taaatcgttg attttcctgc
     4681 ttcgtttccc ccatagatca gctgattgcc tgaataaaag tcaaaacttt tttgacgcca
     4741 ttttccaaaa gcagcaatct cgatccttaa aatcttcatt cctgatcctc ctcaaataaa
     4801 aaatcttgat taagcagctg cttggctttt tcaagtgttt cttcttgata ttccggcagt
     4861 tcgctcaata ttctagccgc ttcttgatga ctatatactt catttaaaat ttgttggaaa
     4921 tcttgaggtg tctgatagta ttgaaataac tgttccatca atttagcaga agcagcaagt
     4981 ggggtcttcg tataaaaagt attctctatg agagaaatac gccaaataaa gaaatcattt
     5041 atcctatctg agaattcctc taacaaatag tctaatagct ctcctgattg tatccgttcc
     5101 aaaacatctg tattgagatg gtcataatct tttaatttta tctcaattaa actaaatcca
     5161 tcctctactt ttgaaagctg ctgacgaatg tgaacaatga cctcttttgt cgttcttgct
     5221 ttcctcaatg atatttcctt tgtcttccaa tagacctctg ctactttaat cggatgtatt
     5281 tggcaacttc ctttacttaa ttcaactagt agaatagatg tagaagcttg ttccttttta
     5341 gtatgacctt gaggcgcacc tggataaaca attgtttgct tttcatttaa gaccgttggc
     5401 acatggatat gccctaaagc ccaataatcg tatccctttt cttgcatctt tgagggttgg
     5461 aaaggagcat agtttccttt ttgagagacg ccaggttccc cgtggtacaa cccaatatgg
     5521 taatctgtga gttctctaga aggaaattcc atcactttgt ctttttgaat ccacggttga
     5581 cgataactaa atccactgag agagacagtt tctccacttt ttatcgttat tttttttgtc
     5641 tctaccgttt cggatgtaaa taaatggaca tttttaggaa aggagaacca ataccgctct
     5701 ttttgataga aatcatgatt tccgaatatt ataaatacag gtatatggtt cttttctaac
     5761 cgtttcatct gttctgaaaa atgtttctgt atttttaatg aaggacgatt ttgatgaaaa
     5821 ttatctcctg caagtaaaac aaaatcaacg ttatttatta ttgcctgttc aatgatattt
     5881 gataaaacgg tcagattcgt cttcaatagt ttttcctgta cagttttatc aagagttgtc
     5941 agtccttcaa atgaccgatc catatgtaaa tctgccgcat ggataaattt caccattaaa
     6001 aatttcctcc tttttttctt ttatcatacc attttcaatg ttttttttca tcaataaccg
     6061 aaaatttgtt cgtattatgc aaagaaatta cgataaatgg ccgacttcaa aaaataagtt
     6121 tattcgaaaa aaagattgtg acatgatttt tgtcacaatc ctaaatatct atgttatatt
     6181 cgcttcgttt gacactgaag aagtactatt tgtactggaa tttatgctcc atttttccgt
     6241 atttagaatt ctatgttcta tactacctta attagtttat atggtaagaa ttctttcaaa
     6301 tgcttgagat actccgtctt tatcatttga aacagtgaca taatcagctt ttttcatgat
     6361 ttcttgatga ctatttccca ttgcaaaact aatatctgca ctttcaaaca tagataagtc
     6421 atttaaacta tctccgatcg cgattctcaa aggtttcttt aaattgtaga ttgattccaa
     6481 acgatcaaac accacacctt tagatacatt ttgaggtacg atttcaatat ttacaggtgc
     6541 cgattcagta acagacacat ttagcatctt tttcaaatgg ttaaatatat tagctttcaa
     6601 ttgacgatta ggaataaaaa taaaaaattt gtaaatgctg atagcatcct tttctata
//
LOCUS       DBKOIJ010000142         6643 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000142 DBKOIJ010000000
VERSION     DBKOIJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.145"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(1..859)
                     /locus_tag="ACV5NJ_002319"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACV5NJ_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACV5NJ_002320"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACV5NJ_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HHY6486363.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACV5NJ_002321"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACV5NJ_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="ACV5NJ_002322"
     CDS             complement(2603..3421)
                     /locus_tag="ACV5NJ_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HHY6486364.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACV5NJ_002323"
     CDS             3582..4271
                     /locus_tag="ACV5NJ_002323"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HHY6486365.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACV5NJ_002324"
     CDS             4285..5787
                     /locus_tag="ACV5NJ_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HHY6486366.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACV5NJ_002325"
     CDS             5800..6276
                     /locus_tag="ACV5NJ_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6486367.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACV5NJ_002326"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACV5NJ_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2174 a         1032 c         1203 g         2234 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DBKOIJ010000143         6625 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000143 DBKOIJ010000000
VERSION     DBKOIJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.146"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(55..1083)
                     /locus_tag="ACV5NJ_002327"
     CDS             complement(55..1083)
                     /locus_tag="ACV5NJ_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486368.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1138..1212)
                     /locus_tag="ACV5NJ_002328"
                     /pseudo
     CDS             complement(1138..1212)
                     /locus_tag="ACV5NJ_002328"
                     /inference="COORDINATES: protein motif:HMM:NF016901.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1215..1571)
                     /locus_tag="ACV5NJ_002329"
     CDS             complement(1215..1571)
                     /locus_tag="ACV5NJ_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486369.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1869..2843)
                     /gene="sgrA"
                     /locus_tag="ACV5NJ_002330"
     CDS             complement(1869..2843)
                     /gene="sgrA"
                     /locus_tag="ACV5NJ_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HHY6486370.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(3039..3878)
                     /locus_tag="ACV5NJ_002331"
     CDS             complement(3039..3878)
                     /locus_tag="ACV5NJ_002331"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HHY6486371.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4063..4950
                     /locus_tag="ACV5NJ_002332"
     CDS             4063..4950
                     /locus_tag="ACV5NJ_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HHY6486372.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5544..6239)
                     /locus_tag="ACV5NJ_002333"
     CDS             complement(5544..6239)
                     /locus_tag="ACV5NJ_002333"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HHY6486373.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6223..6621)
                     /locus_tag="ACV5NJ_002334"
     CDS             complement(6223..6621)
                     /locus_tag="ACV5NJ_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HHY6486374.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2088 a         1306 c          973 g         2258 t
ORIGIN      
        1 aagaaaataa tctttttgca atgtcttaat tttggcataa acaatctgct tttcttaatt
       61 aacgaaatcc aatttttcca atatggatct atttttctca ttcctgattt ttatgatttc
      121 agctttcaga cgcgcccaat cattttttgt aggttgtcca ttccagtaaa atatctgtgt
      181 attcgtttga tcatatgcct ttttgtcgat aggataatcg ctaatgacaa tatcaggtaa
      241 ctgatcagcg gatgcactat attttattgg gcgttcagtc atagataata tccttttctt
      301 tgcccataat tcctgtgttt taccatactt tgattgaata tagaatatga caggttgttc
      361 gtgttttata aaaatatccc tgactaacat acagtattga aaaatcaacc gtctgttctt
      421 ttcataataa gtattaagct ctggaattct gcgagctacc ccactaaaaa actgttgcat
      481 gattggatac atcactggaa atatatgctg aaaatctctg tcttctgctg attttccaaa
      541 agcatccacc ttcttttttg tcccaaaaac ccttgaatac aaatggatca ttgccaaatt
      601 gacatataga taattttttt ctgaagttgt aaaacaaatc ttgaagtttt tttcaaaatt
      661 ttctacaata gacgattcaa tccagcccca actttcttgt actttatctg gagtatccaa
      721 caaatcttct tccaaatagg tgttcattac accaaacata aaataaaggt aagcttgctc
      781 tgttggagtg atgtgattca tcgaccagaa gtttgcttca aaaaaaggct ctagtatgta
      841 atgcgtaaac tcttcttttg ttatcaatgg atgcacaaat ttgttcatct cttctgggac
      901 atacgtaatc gagtggcctt gtttcattcg ttgcaacgaa attcttagta ggctgatcca
      961 tttctttcta tctatactac gcgtatatgg cgcatgtttc gcaaattttt ctaatagttg
     1021 acgtttattt tttatacttg ataatatatc agaatgattt tcgtcgaatg actgccaata
     1081 catcaagtga aaaaagtatc ggatacggta ttcttctcct tctatcggat tacctttttt
     1141 caagctgcaa gataaatgat agtttttcaa aatagtatta agttctctta attttctttg
     1201 aatcgtacgc aaacttatat aattttttga ctaagatttt ctaaggattg ataagtacct
     1261 ttgtaaagtg aatccagtaa aatataacca atcgttccct ctaaaagtat agaataaact
     1321 ttactcagta aaaactgaga agaaaactcg acttttatca tcttgttttc tgaaagaaaa
     1381 gataatttat attctggata cctttcgcaa aacgattctg cttgttgaat caccgaattc
     1441 atcttagcca gggtgtagtt tagatctaaa gcgatatcct tcaccacata taatttctcg
     1501 tctctttcat aacaatagtt caaaaattga tatacttgcc tttcattgat atcaataaat
     1561 tcttcaaaca aagtacatac tcctttttat ctattattta gacttattcc gacagctaca
     1621 tgaataacgt acaaatagat tcgttactga tttttcagag atactaccat gcgcagtgtg
     1681 cattgtcaat tattcttgct cttattttcc atttgtgttc ctgttatttc ctgtttctca
     1741 aatatgttag taaacaaaaa tagatctggc aaagctacaa cttcgccagg caatacagca
     1801 ctctcgaatg aaatattcac tcaaaaatct atcgttttat tcaattttag atctgttgct
     1861 tttattcttt atttcactcg atggaagaga gaacgaaaat agcttgctcc tacaatcaaa
     1921 atgagtaaca cacccatcca tgtaattagc ttgttcaagg ttctactacc agtttctggt
     1981 aaatcgcttt ttctattatc tgtttctttc gctttttctt gatgatcaat tccacgagaa
     2041 acaaaatcag gctgttttag cacacttttt aggttgctcc ctgcatttct cttttgttcc
     2101 ttagttggta tgataggtac caaatcaagt tcattattta cttttcctgt gctgctttcg
     2161 ctgctcgtgc ttcctgtgct actctcacta ctcatgcttc ctgtgctact ttcactactc
     2221 gtactctccg tactgctttc gctgctcgtg cttcctgtgc tactttcact actcgtactc
     2281 tccgtactgc tttcgctgct cgtgttttct gtgctacttt cactgctcgt actctctgta
     2341 ctgctttcac cgctcgtgtt ttctgtactg ctttcactac ttgtgctatc cgtactgctt
     2401 ggcggtccaa caactgtcca actgcttggt ttcaaattct ccgtgatcgt catctgtcca
     2461 tcataaaaaa caatcaaatc aactttatct ttactcttga tctcatcaat cgatagttct
     2521 gcagaaatca ttattgtata ctcaatacca gcggtccatt gtacgtcttt taaataaact
     2581 tccccaataa ctttttctga acttccatta ccatcccaac catatgttag aacagcatgt
     2641 acatcactag cattcacgct tttgtttggt ttgactggaa tagtaatgac ttgttgtgtc
     2701 atgtcataaa caatccgatc agttttcaca gtaaatgaaa gattctcatt tgcccgttca
     2761 tttgcagaag tactgattcc aatcattgct gcaattatac tcagcccaag aataacaaat
     2821 aatacggtcg ctgttttctt catttctatc actctccata taattacctt ctatttgata
     2881 aaggaattat ataaaatgaa aatgatgaga acacgacagt cagttgtgaa aatctaacaa
     2941 aagatgtcgt gtattcgctt gaatttgttt actttttcgt ttaatcaatg acagacttaa
     3001 aaaagcaaga ccgatatcag ccttgctttc ttcatctttt attgataaac agcagtgtat
     3061 ttcttcatcc gctttgtttt atatgcatag agtaacaaac caacaatcaa gactaacaag
     3121 aataatgtag ttgatataga tgcttcagcc ccaaagtttc ccccagtaat tattgtattt
     3181 tgcgattgga tcagagtgga gaaaaaacct tgttcttgcg ttccgctgac tggtataccc
     3241 aaaatagctc cttgtgtcca attccaagta atatgagcac caatagccgt aaataaactt
     3301 cctgtactgt agataagtgc gctgaaaaag caacctgcta aaaagatatt gaaaactgcc
     3361 agaaatgtca tgttaggatt caatccgtgc aaaagagcaa ataatgcagc agatccaatc
     3421 attgctatta acggaccaaa tctaagcatc tgatgctgaa aaaagccacg gaataaaagc
     3481 tcttctgatg cccccactgc aataaataaa ataaaactcg ttatcagcgg cacaatgttt
     3541 gcgtttcctg aatctaaccg atacgccccc gtcaaatata atggactagt aataagcaga
     3601 aacactccaa ttccaacaac gagacctccc gctaaatacc cccagtcttt tcctcgaaca
     3661 gaaacaccat aatcttctat tctttttcct tcgaccttac tagcaagaaa taaagctagt
     3721 aaaaaaacag ctaaatgtcc gaccgtaccg gcaattgctc caatccattc actatttcca
     3781 ataaacatat ccgcaagtgc attattgaca agaattccta aaacaaaacc aactaagact
     3841 attaaaacag atccaatcaa ccgaataatc ctgatcatct ttcatccctc ctttatatta
     3901 agtttaacaa cccttttctt tcttgtctcg tgaaaactat gtaaatgttc tccatcatag
     3961 agagaataga tttttacgaa ctaagaatat ctacagcaac cttacgaaaa tgaaagactg
     4021 tctccctaat tcttctgtta gactgttttt aataaggaaa ggatgggaaa ttttttgaaa
     4081 aaacaactac taattatgtg cctttcagca tgtgcaattt tactattctc aggttgtagc
     4141 aagtccaatg aaacgattgc aacgtttaaa ggtggaacac ttacagttca ggatttttat
     4201 gataacccaa aagttaaatc aaataatcaa acactattaa aaaaaatgat tgtcttcaaa
     4261 gcatttgatg atgtatacgg aaaagagatt tcttcaaagg aagtaacaaa agaatataat
     4321 gaacaaatca aaaaactcgg tccaaactac aaggaacaat taaaagcagt tggccaaaca
     4381 gaagagacat ataagaaact ctttaaacag atgctggctt tccaatacgg tttgaaagcc
     4441 aatgtaaaat taacagataa agatctagac actgcttgga aagaattcta tccagaagtt
     4501 agtacacaga tcattctatt ttcgactaaa gaagaagcag ataaagcaaa aaaagaagca
     4561 aatgaaggag aaaatttctc caagcttgtt caagcttatg gaaaaaataa aacattaaag
     4621 gaaactgatg gaaaaatgaa ttttgattca accaatccag agattcctac tgaagtaaaa
     4681 aaagcagcat ttaaactaaa aaacggggaa gtttctgata tcattcctgt aacagatcca
     4741 acgacttatc agcaaagcta ttatctagta aaaatggtaa aaaaacagga taaaggttct
     4801 aataaagaca aatacaaatc tgagctagaa aaaattgcta ccgaagcaaa aactcttgat
     4861 acggaattca tggataaaac aatccgtaaa gtgatgaaaa aagacaatgt aactattaaa
     4921 gatccttatg taaaaaatat attcaaataa acaaatttga cccaattggg gatgtggcaa
     4981 aatttgagaa gtgatttttg gaaattcctt cttatttttg aggcaaacca cttttgccac
     5041 aacttcatcg gggccttttt ttatttgtac caaaacaaat cactggattt gttacaatca
     5101 tgaggatata atttaaaaaa gttttttatt tctatcacat gaatcaatgc gccattaagt
     5161 agattctttc atgcttccct attttttcac ttgccgtatc gcttcaaaaa caactatcat
     5221 aaactcaaac tcgactgaaa agttatttta aaaaagacta aaaatatccc ttctggactt
     5281 gttcttctta ttctgttata ctagagaaac acgaaagata tttttatgcg acacttttca
     5341 tttgttttta tgaaatttat tcaaaactgg cgtaacttca gagttacaag gatgtattag
     5401 aggttaggga atacatcgtt taaatgagaa ataagtaata gttagtaata gtcacccaaa
     5461 ctttaaatta atgaatattg aatcgtcaga tagcctcctc gagttttcga gaaggctatc
     5521 gtttatttta tggagaaatt gattcagaag taatcagcga ccttttttag ttcaccaatc
     5581 gtttctgctt ggaaagtttg cacccaatct tcttggaagc ccctcactgc tttatcaatc
     5641 gagggcatct ctaatacttg atctagtagc tctttaccta acgttatcgc atgagctcca
     5701 gatcgaatgg ctgtagtgac ttgttcacaa tttttgaaac tagctgctaa tatttttgtt
     5761 ggtctttgcg tacgttcgat ctcagtccgc aacgaatcaa tcactttcat agcatcaatg
     5821 ccttgatttt gcatcctgtt ataataaggt gcaatataat ctgctccatt attaatagct
     5881 aaataccctt gtaattctgt gtaaatagct gtaccggtaa tcaaataacc ttcttttttc
     5941 aactttttga tcgcttgaag gcctgcttga ttcaccggca ccttgataaa cgtatctttt
     6001 cccagttctt ctatgataac tcgagcatcc tctatcattt cttcttccgt tcttcctatt
     6061 gtctgaatat ggataggaat ctgtccgatt atttttttga tttctttcat atgcgagaag
     6121 aagtttgacg ctccttcttt tttcagaatt gtaggatttg ttgttacgcc tgttaaattc
     6181 aaaaactgtt gacaagaata aatacttttt gtatttgctg tatcaagcat gatttccata
     6241 tagctttccc ccattctgat cctcttgatc gattttaatg attgtcccct ttcttaaatg
     6301 cggcatttcc ttatcttcta catacattgt tcctggaagt tccggctcat gagatccatc
     6361 aaattttatc gtgatatgtc ctaattcatt caaattagtt tggacaacat ctcctactgc
     6421 tgtgattaca tacatttgtt tatcaaaaca gagcttcata tcacaagtta tttcttttct
     6481 tacctgattc aattcaatct tataacaata atctgttaat gtttccggtg cttcatctcc
     6541 aaataaaatc attatttttt ctttcctgaa tacttctgct tctataccaa catctgttac
     6601 aaccgtttca aagatcgtca tctct
//
LOCUS       DBKOIJ010000144         6604 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000144 DBKOIJ010000000
VERSION     DBKOIJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.147"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            130..510
                     /gene="gloA2"
                     /locus_tag="ACV5NJ_002335"
     CDS             130..510
                     /gene="gloA2"
                     /locus_tag="ACV5NJ_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
                     /protein_id="HHY6486375.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            606..1115
                     /locus_tag="ACV5NJ_002336"
     CDS             606..1115
                     /locus_tag="ACV5NJ_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486376.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            1192..1371
                     /locus_tag="ACV5NJ_002337"
     CDS             1192..1371
                     /locus_tag="ACV5NJ_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486377.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            1426..1989
                     /locus_tag="ACV5NJ_002338"
     CDS             1426..1989
                     /locus_tag="ACV5NJ_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6486378.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2036..2551)
                     /locus_tag="ACV5NJ_002339"
     CDS             complement(2036..2551)
                     /locus_tag="ACV5NJ_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HHY6486379.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(2577..3446)
                     /locus_tag="ACV5NJ_002340"
     CDS             complement(2577..3446)
                     /locus_tag="ACV5NJ_002340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHY6486380.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            3572..4252
                     /locus_tag="ACV5NJ_002341"
     CDS             3572..4252
                     /locus_tag="ACV5NJ_002341"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HHY6486381.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4405..5385
                     /gene="pta"
                     /locus_tag="ACV5NJ_002342"
     CDS             4405..5385
                     /gene="pta"
                     /locus_tag="ACV5NJ_002342"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HHY6486382.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            5508..5981
                     /gene="tsaE"
                     /locus_tag="ACV5NJ_002343"
     CDS             5508..5981
                     /gene="tsaE"
                     /locus_tag="ACV5NJ_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HHY6486383.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            5974..6498
                     /locus_tag="ACV5NJ_002344"
     CDS             5974..6498
                     /locus_tag="ACV5NJ_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HHY6486384.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
BASE COUNT         2187 a         1169 c         1391 g         1857 t
ORIGIN      
        1 tactttctgt tttttttgcc ttttttctta taaaaaggaa aaacagctat ttttttcaat
       61 ctgacacatt tacaatggaa ctggagatta atatagatcc gagcttaaac gaagaatagg
      121 agtgctcata tgaaccttac aacgatccat catatagcca tcattgtttc agattatcag
      181 aaatcacgtg atttttatgt taataaatta ggatttgaag taatcagaga aaattaccgc
      241 aaagaacgag atgactataa attagatctg aaactaggaa gtgctgaatt ggagattttt
      301 ggaattgcta atgcgccaca acgaccaaac tatccagaag cttgtggctt gcgtcatctt
      361 gctttcaaag tagacgatat tgaagaaaca attgcagaat tgcagtcctt agggatcgaa
      421 tcagaaccga ttagagaaga tacctttaca ggaaaaaaaa tgacgttttt ctttgatcca
      481 gacggattgc cattagaact gcacgaataa agataacaaa attgtcactt ttttttcgtt
      541 tcattcgatt tttcattcgt tcatttcttg atattcttta aaaaaaagaa acaggagaat
      601 gaagaatgaa aaaaatgatg tttggtgttg ctgccctcat cgtaataggg attaccggta
      661 tgacgggact gctcaatgcc gcttttcaca tggatgatat tagtgaccag gaatatcgga
      721 tctcattgaa aaaagcagca gcaatcgtaa aaaacgaaac aaaagaacat aacatatctg
      781 ctattcattt tgcagttgaa aaagatttgg agaatcctac gaatgcaaca tatgaatacc
      841 agtttttttg tgaaggacaa gtcatagcag tcaatccatc cactgggaaa acaaccgtac
      901 gaaatgacac ggaaaaggcc gcagaacatc tttttgatat agaaaaaacg gaagagttca
      961 aacaaccgca agcagcgatg aaggaagcag tcagctacta cgggaaaaaa gcaaaagtga
     1021 aagagtggac acttctagtc aaagatgaac gtccttatta taaagtccga ttaatagatg
     1081 gagaacagga ccgtcaggta tggatatctg catagaagaa ggatattgct tctctttttt
     1141 acaatataag agtaagctaa gagagtaaag atgaaaggaa gttgagataa catggaaaaa
     1201 atgaaatgcc ctaattgcgg aaaaaaattt gcatacgaag aagtcaataa tgtagtcgaa
     1261 cataatgata aagaaatgcc agtcgtctgc ccttattgcc gaactgaagc ggcaagaatc
     1321 gttactcatg gttatttcat tactgaaaag attgaagatt ttttaaaata acgattttgc
     1381 gatttctttt tctgcatgtt agaatggaga aaaggagagg aaactatgat tcgttttgcg
     1441 agaaaagaag atgctccaga aatcgcccca ttgatcttag tgattctaaa agatatggaa
     1501 ctgccatttt tgaagcagac aggggaacaa aagactttag agattttaga agaagcaatc
     1561 actgatccaa attatcgtta cagttattca cgaggtatcg tagaagaaat agacggtaaa
     1621 gttgcaggag ttgcttacgg ttatacagat gaagaagaat caatcatcga tcagccactg
     1681 gtacctatct tagagaaata tcagctagat gcgtctacac gtttgttcat tgataaagaa
     1741 acgtttccca acgaatggta tttggactct atcagtgtat cagaagattt tagaggacaa
     1801 ggcatcggtt cgcgattatt agaagcatta cctaaattgg cgaagaaagc taatcgctcc
     1861 gtcatcggtc taagtgtgga tgaaaaaaat ccaaaggcta aaaaattata cgaacgccat
     1921 gacttcaaag ttgtcggtca gcggatgatc agtggtcatc tttatgatca tatgcaaaag
     1981 aatatctaaa aagatgtacc tgaaatgcgt atttccgcat ttcaggtaca tctttttaac
     2041 ttaagttaag tcggtaagaa acaggcttat cgcgactttt gtctttacgg tagagccagt
     2101 aatctaaaac cgcagcaata caaatacaaa ctggagcatc ttcatcattt gagacatcca
     2161 atacatagta atctcctgaa aaaagtgttg ctttatccat tttcatgatt tgctgattga
     2221 aatgatggat ttgatattga tgattataga tatcaccttg gactgtccag tgaagtctac
     2281 gaatataata aaagtcaccg ggccaattga atattttctg catggttccc accttttcga
     2341 actctttata cagctcaaaa cgcgtaccga atgtcaggct tatctgcttg atgtgagcaa
     2401 cgagatcgcc attcatggca taaagagaca atgcatcgcc ctttgttccc cagcgaccaa
     2461 ccatcaaaaa cagggaatgt cctttttcgt ttttgacaat tgtccgagtt gcacgtccaa
     2521 gctgcttttc ctgaatgaaa aattctgaca tctatatccc tcttttctag ataaaactac
     2581 aagtcttctg caatcgcgcg caatatggct tttcctacgc cagattcaag gtttgtgtcc
     2641 gttgtatatt tggcataatc ttttacttcg atttctgcat ttcccattgc gaaactgact
     2701 cctgctactt gcagcatgct gacatcattg aagttatcgc caatcgtcat gacttcttct
     2761 aaagaaatcc cacgctcgtg agcgacatgg gcaacagcaa ttcctttttg agcgttttta
     2821 tggttcactt cgatattgtt ttgaccagag gaagtaacgg ctagatcgcc taaaagagaa
     2881 atctgctttc caactggacc taatacagct ggcccttctg tatgaaaaca aataattttt
     2941 aaaacttcca gattcttttc ttccaaaata gcacgaatac tgtccacgta tgtgatgtgc
     3001 aataactcca gcttagctga ggccatggca atcgccattt tatacgtaag gtgaggtaaa
     3061 tgggtagcca ccatggaagc aaaactttca attcgtttcg cttgattttc agaataaaga
     3121 ccatcgttgg ttgctacttc atagtaaata ccgttagcat ctagaatatc taaaacagag
     3181 attgcttgat tatttggaat cgacacggta aacaatgaat ttccttcttt atcaaatacc
     3241 tgtgccccgt tcaaagtgat catcgcacaa tcgattccta cctcatctaa agctgcgcga
     3301 gcttcttgat agtttcgacc agtagcgacc atgaactcaa caccggcatc atttgcaaaa
     3361 cgtatcgcct cggcattttc aggagaaaca ctcatatggg catctaataa tgtgccgtcc
     3421 atatctgatg caattaattt gatcataatg attatcgttc ctttctgctt cttgttccta
     3481 gtttaccata ttaatcaagt gctgagggtg aagattgtcc cttgtgattg aagaaaatgt
     3541 gagttattct ttttattagg aggtggagtt tttgaaagaa atcattcata acagttggca
     3601 agaagttttg tcctcagaat tttcaaaaga ttattatctg catctaagag aatttttaaa
     3661 aaaagaatat gcgtcccaaa agatccatcc agatatgtat catatctatg aagcattgga
     3721 attgactcct tatgaagaag ttaaagtggt gattttagga caagatcctt atcatggaga
     3781 aaatcaagct catggcttat ctttttctgt gcaacctggc gtaaagatcc cgccatcgtt
     3841 aagaaatatc tataaagagc tttatgatga tcttggaatc gcacctgttc aacatggaaa
     3901 tctagtgtca tgggctaaac agggagtcct tttactcaac accgttctta ccgttcggga
     3961 agggcaagcg tattctcatc gcgggaaagg ttgggaaaga ttaacagaca cgatcattga
     4021 aaaattaaat gaacgggaaa aaccaatcgt gtttatcttg tggggaaaac cggcacaaga
     4081 aaaaatcaaa atgatcgata agagtcgcca tatcatcata acatctccac atcccagtcc
     4141 gttatctgca agtcgtggct tttttggttc caaacccttc tcgaaaacga atgatgcgct
     4201 ccttgcgtta ggagaagaac cgattgattg gcaactgcct gaaacagtgt aataaaacag
     4261 cgctttcctg ttatagtgca gttcgctttg tatgtgaaaa acttaatttt tccagcattt
     4321 ttgactgata cagataaaat aaactgttct ttttttctgg aatagtgtat gataatacat
     4381 ataaaaacgt ctggaggtca tcttgtggaa ttgtttgata gtttaagatt taaaattgtt
     4441 cgccgtggga tcaaaatcgt tttcccggaa gctactgatg ctcgtatttt aggcgcagct
     4501 gcccggttga aagcagaaga acttgtggaa cccattttag tagggaacga agaagaaata
     4561 agaaatgcag cgcatgccag aggaatcaaa acatctagct ttacgatcat cagccctgat
     4621 aattacgata agtgggatga aatggtggat acttttgttg aacgtagaaa tggaaaggca
     4681 tccaaagaac aagcagaaaa gattttaaaa gatgtgaatt actttggtac gatgcttacg
     4741 tatatgggga ttgcagacgg aatggtctct ggagcgatcc attcgactgg cgacactgtt
     4801 cgcccagcac ttcagatcat caaaacaaag cctggcatca gtcggacaag cggagcgatg
     4861 atcatggtac gtggaagaga tcaagaaaaa tatgtctttt cggattgtgc gatcaacgtg
     4921 aatccaactg cgcaagaatt ggctgaaatc gccgtagatt ctgcaaaaac agcagaattg
     4981 ttcgatatcg aaccaaaagt agcaatgatg agcttctcga caaaaggctc agcgaaatcg
     5041 ccagaagtag acaaagtagt tgaagcaact aaaatcgcta aaaaactagc gccacaatac
     5101 atgatcgacg gcgaattgca atttgatgct tcttatgttc cttcagttgc acaactgaaa
     5161 gcgccagatt cagaagtagc tggtcaagca acagtctttg tatttccaga attgcagtca
     5221 ggaaatatcg gctataaaat tgctcagcgt ttcggtaact ttgaagcaat cggaccaatt
     5281 ctacaaggct tgaacaaacc ggtttctgat ttgtctcgtg gagcaaatga agaagatgta
     5341 tataaattat cgattatcac agcagcacaa acattgattg attaacaaat acacgcgaac
     5401 tagccaaaaa cagcagctgc tgttcttgac tagttcattt tttctttaaa aaaacttcat
     5461 tacgcgatat actggcaaaa agaaagaaga gcgggagaag atgaaaaatg atcgaactat
     5521 caggattaac aatgacagaa caattcgcag aggcaattgg tcattccgca atgccaggtg
     5581 acaatctgat attgactggt gatctaggtg cgggtaaaac aacgttgacg aaaggaattg
     5641 cccaaggatt agggataacg caaatgatca aaagtccgac atacacgatt attagagaat
     5701 atagtcaagg tcggattccg ttgtatcata tggatattta tcgggtagca gctagcggag
     5761 cagatttagg gttagatgaa tattttgaag gtgatgggct ttccgttgtt gaatggggaa
     5821 atctattaga agaagcactg ccggaggatt atttggaact gattttagaa aaaagtgata
     5881 cagatttaga aaagcggtat gtgaaactaa tctcatacgg ggagcaaagt gaaaccttca
     5941 aacagcgaat tctagaaaaa tgggctgaaa atcatgagtg aagaagttgg gatcatcttg
     6001 cgggaagcag tgccaggaga tgcaaaagac attcttttga tgatgggaca agttaataag
     6061 gagacagaat ttttagtttt ggatgaagca gaactcctgt taccaccaga aaccttagaa
     6121 gaagagttgg attatatata cgaaagcaat aataatttat tgctgcttgc aatctatgaa
     6181 gggacgatta ttggaacagc atctgtcaag gcagacagtc aatttcgttt gtctcacgtg
     6241 ggagaagtcg gtatcagtat tctgcaggaa tattggggaa tgggactagg cactcttatg
     6301 ctagaagaaa tcatcagttg ggcgaaggaa atgggtgtac tttttcgatt ggagcttgac
     6361 gttcaagttc gtaatgaaag agccgtacac ctttaccgaa aaatgggctt ccaaatcgaa
     6421 gcggtcatgc ctagaggggc aagaacagat ctaggtgaat ttttagatgt atataaaatg
     6481 agttatttga ttgaatagaa gaaataggaa aagagtgaga aactgtcatc cttttgagaa
     6541 ggcagaaaaa cacacgaagg gactgtgaca aaagtcgtgt cgcagtccat tttttttgaa
     6601 taaa
//
LOCUS       DBKOIJ010000145         6597 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000145 DBKOIJ010000000
VERSION     DBKOIJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.148"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            66..752
                     /locus_tag="ACV5NJ_002345"
     CDS             66..752
                     /locus_tag="ACV5NJ_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HHY6486385.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            754..1698
                     /locus_tag="ACV5NJ_002346"
     CDS             754..1698
                     /locus_tag="ACV5NJ_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HHY6486386.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1897..2541
                     /gene="pcp"
                     /locus_tag="ACV5NJ_002347"
     CDS             1897..2541
                     /gene="pcp"
                     /locus_tag="ACV5NJ_002347"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HHY6486387.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2620..3897
                     /locus_tag="ACV5NJ_002348"
     CDS             2620..3897
                     /locus_tag="ACV5NJ_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486388.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4155..5372
                     /locus_tag="ACV5NJ_002349"
     CDS             4155..5372
                     /locus_tag="ACV5NJ_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHY6486389.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(5693..6442)
                     /gene="pnuC"
                     /locus_tag="ACV5NJ_002350"
     CDS             complement(5693..6442)
                     /gene="pnuC"
                     /locus_tag="ACV5NJ_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HHY6486390.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2123 a         1218 c         1365 g         1891 t
ORIGIN      
        1 actatatttt ttatgtgttt tgctttataa ttaaaaaaat attgtcattt tatggaggaa
       61 aatatatgga aaattatctt agccttttag gcgtcttgat tgttattctc gggtttgcat
      121 ttaaattaga ctcgatattg atcgtaatgg ttgctttagt tgttacagct ttgactggcg
      181 gtcttggcat tcaaggaatg ttagaacttt taggtacaag ttttgtaaac aatcgcggga
      241 tggcgatttt tatcatcatt atgttggcaa caggaacgct tgaaagaaat ggtctgaaag
      301 aatcagcggc ttccttgatc aagcgattca agaaagtatc tgctggtatg attgtagata
      361 tttatgggat ctttcgaatg atctttgcgg cttttaacgt gagttttggt ggagttgctg
      421 gatttgttcg tccaatcatt ttaccgatgg cattggggac catcgaatca aaagggttag
      481 aagtccatcc taagcatgaa gaagaactaa aaggtatggc ttcagctatg gaaaatgtct
      541 gctggttctt tggccaagta ctattcatcg gaggagcagg ggcgttgcta gtccaatcaa
      601 cactgaaaga tctaggatat gacgtaacat tgggacagct tgctttagtg gaagttccag
      661 tggctatcac ggctttagtt gtggcaagtg tctacttcta tcttaaagaa aagaaattag
      721 cgaagaaata ttatccagga aaggggcaat aagatggact tttttacaaa tcctgatatt
      781 ttattaggcg ataaattact ggagatcctt tatatcgtga tgggactcgt attgatttac
      841 actggagtaa gaaatttaac agataaaacc aatcctcatc gttatgggtc agcctttttc
      901 tggacggtct taggggtagt tattgcagga ggaagatggc ttccggcaat cgtgaacggt
      961 ggcttgatct ttgcgatgac cataccagca attgctaaga aagtcagcaa aggagaaagc
     1021 cggcttccat caaaagaata tatggaaaaa atgtcagaca aactaggaat gaaaattttt
     1081 attccagctt tgagtatcgg tgtctttgcg atcttgtttg ctctatttac caatttaggt
     1141 gctttagtcg gcgtgggcgt cggtgttttt gcagccatgc taatcttgat gttcttttct
     1201 cgagataatc gaccaagtac atttttagat gatgcagcag atatgctggg cacagtaggg
     1261 ccgttaagca tgttgccaat gcttttagct tctctaggtg cggtatttac aagcgctgga
     1321 gttgggacag tgatttccaa tgcagtaggt acgatcgttc cagaaggaaa tgtgaacatc
     1381 ggcatcatca tttacgctct tggcatggtg atatttacgg taatcatggg gaatgcattt
     1441 gcggccatca ccgtgatgac ggttgggatc ggtgcaccat tcgtatttag tcatggagcg
     1501 aatccagcgt taataggaat ggttgcattg acttgcggtt tttgtgggac cctgctgaca
     1561 ccgatggctg ctaacttcaa tatcgtgcct gtagcgatgc tggagatgaa agacaaatac
     1621 ggagtgatca aaaaccagtt gtttattgcg ttatttatgc tagttttcca aattgcttat
     1681 atgattctat ttaaataata gaagagaggg gttgtgacag aataattgtc gcaacctctt
     1741 ctaacattgt agtggaacac tattttttta taagaatata ttaagtttga tctgttatgt
     1801 tatttaaact attgtttcct ttatttacgc tgttcaagga aaatggtatg ataaaagata
     1861 accatatgtt aaatcaatcg gacggaggaa caacagatga aagtactagt aacaggtttt
     1921 gatccatttg gtggggacaa agtgaatcca gcctatgaag cagtaaaaaa aatgccagat
     1981 gaaatcagcg gtgctgaaat catcaaagtg gaagtaccga ccgtattcga aaaaagcagt
     2041 caagtagtca aagaagccat ccaacagcac caaccagatg tcgtgatctg tgtcggacaa
     2101 gcaggtgggc ggtcagcagt ctcgttcgaa cgtgtcgcaa tcaatctagc agaagcaaga
     2161 atccctgata atgaaggaaa tcagccattc gatacggctt tagaagaaaa cggtccagcc
     2221 gcttacttta cctcattgcc aatcaaagca atgacaaaaa atgtacaaga tcatggtcta
     2281 ccagcgtaca tctcttatac agcaggaaca tttgtctgca atgatatcat gtatcgtttg
     2341 cttcatttga tcgaaacaca attcccggca attcgaggtg ggttcatcca cgtgccattc
     2401 tcacccgatc aagtgatcga tcgtccagtg ggtacgcctt ctatgtcttt agaagacatt
     2461 gcttcttctt tgacttatgc agttgaagca gcagtagaaa ataaagaaga tatttccgga
     2521 aatgccggaa caactcacta atttagcaag aaacactggc tggttgctaa cgttatgtta
     2581 gtagccagct ttttgtaatt agaaaggaaa gaaacagccg tggaagaatg gcaatccgtc
     2641 tttgaagaat ggtttccaaa agaaatcagc aaaagctatc ccatcaaaat ttctaaacaa
     2701 tacacaagca gtcaacgttg ggagatctat gcaaaattaa cgaaaaaaca gcgggaacta
     2761 gtcgataagc accgccgata tctaatcagc tctcgtttta tggaagaaca ttatttagct
     2821 gcaacagatt gggtgttttc tgacttcaag atcaatccgt tttttcgaac aaaacgaagc
     2881 cagcaaaaat tgtattgtga gtgtggtaga gaattaaaag tgcaatacat cgttaaatcg
     2941 ccaaaaaccg gaaagatcct aaaactaggc atcaaccact ttgccgatca cctgcatgtc
     3001 tctccgacag tggcagcttc gatccatcag ggaatgacaa aagttgatct ggccttagac
     3061 gaattattat ggcttaagca gaagaatatc gattttcctg aaggactatg gcaaaaatac
     3121 tgttttgtct tgtaccaaaa tcgtcggatg aaacagccat atttaccgga tataaaatta
     3181 gctcagcggc tcgcagaatt tcgccaagta gaaatgccga tttacatagc ggattatcaa
     3241 gcattggaaa atgaaatcaa gaaaataagt gaacacatca atgggcagtc aaaaaagcga
     3301 cagataaaaa aagaactatt tgacgacttt gcagaagaat tagtaaagga tgtagaagaa
     3361 ttcttgatca actatcgcgc atttttacgt aaagattggc aatccatcgt atatgaagaa
     3421 gtgccagtac atcctaatgc ttattttgaa acatttattt ccgtgttgag aaagacaaaa
     3481 cgtcaacgaa caccagaagt cactgctcag atggaatatt ttgcgaaaaa ccaacgattc
     3541 atccaaccaa aaatctatct atttatttgg aagcaatatt gtcgctatgg ttttacagaa
     3601 gggttcttcg acagcattcc tcggatagtc agaaatggtt ttttaaaagt attgcgtaaa
     3661 gaacgagaag ctatacaatc tgctgataag aaagaccgta cagtctcaaa agaaaaatgg
     3721 caactagtag tgaaagatat ccaatctgga aatgtgcaag agacaatcga caaatggaaa
     3781 ggaaaacatt atcgttttac tgaagctcag aaacaagcgt tggaatatta tcagaagtta
     3841 gaagaatcac tgcgtttcaa tgatgaggca agaaagtatc tgaaggaatt actataaaaa
     3901 acatactaag attagagggg aacatcattg acgaatgatg ttctcctcta ttttctattt
     3961 ttagattata gtgaagagtg aaagaaagtt cgaacaaagc cttgaggact acgagtccct
     4021 atcaaaaacc gaacttcttt actgtcattt tagaatcagg tttacgtatg ttggggccat
     4081 gagcccgtgt ataggaaatt ggaatcgaaa tgaagcagta aagttctttc aaaacttaaa
     4141 aaggaggaaa acaaatgcct tacattcttg cattagatgt ttcaatggga cacagctaca
     4201 gtgtcctata caaagacgac acctgcttat ttgaagacga gattgaacac actcaaagag
     4261 gatttcatgc attactcgaa gagatccagc acttgcctga gcgtccattg attgtttttg
     4321 aagcaacggg gatttattcc agaccagtcg aaaagttctt tcaagatcat cacttttctt
     4381 actgtttgtt aaacccgtta gaggcgaaaa aacagatgga agaaagcacc ttacgcagtt
     4441 ggaaaacaga taaatcggat gcccaccggc tcgcacagac tcatcttaaa aatcaacgtc
     4501 agccaaaaga agtacaaagc aatttttact tagagatgcg cgatttagcc cgtttttatc
     4561 aagaaattga gaagaaaatc actcgtctgc gcatggattt acacaactgc cttcaattga
     4621 cttttccaga gcttgaacag ttcttttcaa acaggctgac cccttacgca ttgacattga
     4681 tccgtctttt tccccatcca gattttgtat tggcatctac tcggacaaaa ataaaaaata
     4741 aactgatcaa cgaaacacgg aaaaaaatct ccgcgaatcg tgccgagcaa aaggcagatc
     4801 aaatcatcca ctatgcacag tgtgcctatc cagctgttga aaaagacagt atccactgtc
     4861 aaaaaacgat ctattacgcc gaacttctcc aagatttact cgaacaaaaa aaagcactgg
     4921 cgacacaaat gatcaaaaaa gccgaaggct ctccttgctt tttgttgtac caaacgttcc
     4981 ctgggattgg cgcactcaca gccgctttat tgctcggtga attaggagat atcacacgat
     5041 ttaaaaccca taaacaattg aatgcctttg tcggaatcga catccgacgg tatcattcag
     5101 gtaagtatac tggccaagac cgcatcaata agcgtggtaa tcccaaagca cggaaaatta
     5161 ttttcttcac cattcgaaat atgattcgcc agcaacgcgc agctcctaat catattgtcg
     5221 attattacta caaattaaaa aagcaaccta tccctaagaa agataaggtt gccacagttg
     5281 cttgtatgaa caagcttctg aaatgtatgc acgctatggt tagggcacat acagagtacg
     5341 actatgcgta cgcggtctca gtggaccatt aactaaacca cttacagtat accacttctt
     5401 tgcccagatc gaaaattttt tacgaacggg gctttatttg gtgtgctttg attttgtcgc
     5461 tttcgtttac gagctatttt tcttgcacct tttacttgac taatcgtagg aaatagaggc
     5521 tgggacagaa gcgtttaact ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa
     5581 tttttgtgaa tttcagctta tttccgaagg agttgcttct gattccgccg tttatacgtg
     5641 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctcagttt ttttatgcgt
     5701 gttgttgtgc gttgtagtat tcttcttcca ttttttgcat acgttcacgt tctttttggt
     5761 tataccaagg tgaaacggta aatgcaagga catcattcag caagtaaaca gagctgttca
     5821 cgaacatcgc caaacttgca gatccatgcg taaatgaaat ataccaaagg accatttgag
     5881 caagccctga agctaaccac cagtaatatt ggttgttgta acgcaagaaa ctgatgatac
     5941 cagcagtcaa gctaatagaa aaagcaatgg catctaccca cggccgagga tcatctgtat
     6001 aacgaccaat caggaatcct gaaacaagat agacaaggaa agtactgatg attgcgataa
     6061 accagctttt tccagtgaat tttcggattt ttgaagccat attatgattc cattctttac
     6121 ttaacaatac ggggatatcc aatgtgatca tataagcaat ctgttcaccg atacttaaat
     6181 agttctttgc tgaaaaccca acgataataa aacaaatcgc agacaaaata cctaaaatac
     6241 cgttgactga ttttgccgca ttgatggata aaatacacaa tacaccaagc gtggtaccga
     6301 taaatgtaat aacggacagc caagtgatgg gactattgac aagagtcatg acctggcagc
     6361 caagactgaa aaagaaaaga taataatttt gttgcggcca gccttttagc tgatgagcta
     6421 aaaactgaaa atatgacttc atgtatgagg actccttaag acactaaatg tagtgtgttc
     6481 tgtatttact aacacaatct attgtatgct tttcttgaaa aaaaactagt ctttcaatta
     6541 taaataattt gtgttatgat aattttctgt ttgttttctt gaaaatataa tttctta
//
LOCUS       DBKOIJ010000146         6502 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000146 DBKOIJ010000000
VERSION     DBKOIJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.149"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            166..543
                     /locus_tag="ACV5NJ_002351"
     CDS             166..543
                     /locus_tag="ACV5NJ_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHY6486391.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            531..869
                     /locus_tag="ACV5NJ_002352"
     CDS             531..869
                     /locus_tag="ACV5NJ_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHY6486392.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            1003..1446
                     /locus_tag="ACV5NJ_002353"
     CDS             1003..1446
                     /locus_tag="ACV5NJ_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HHY6486393.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1708..2523
                     /locus_tag="ACV5NJ_002354"
     CDS             1708..2523
                     /locus_tag="ACV5NJ_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6486394.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2533..3195
                     /locus_tag="ACV5NJ_002355"
     CDS             2533..3195
                     /locus_tag="ACV5NJ_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HHY6486395.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3400..5064
                     /gene="alsS"
                     /locus_tag="ACV5NJ_002356"
     CDS             3400..5064
                     /gene="alsS"
                     /locus_tag="ACV5NJ_002356"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HHY6486396.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5077..5787
                     /gene="budA"
                     /locus_tag="ACV5NJ_002357"
     CDS             5077..5787
                     /gene="budA"
                     /locus_tag="ACV5NJ_002357"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HHY6486397.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5917..6414
                     /locus_tag="ACV5NJ_002358"
     CDS             5917..6414
                     /locus_tag="ACV5NJ_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HHY6486398.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         2139 a         1016 c         1379 g         1968 t
ORIGIN      
        1 taaaaaaaca agactttttt atttgaaata atacacaaac aaccgatata gataatccag
       61 cttagtttta atagaaatga gtattgactt ggagtacact taaagttgta gaataaataa
      121 aatacataag taaagtggca agaaattaag agaaagagtg atcagatgaa tatcaaacaa
      181 gtcagtgaag aaaagggcat ttcggccgat acattgcgct attatgaacg aatcgggttg
      241 attccaccag ttaatcggac aaaaggcgga atccgtgatt atacagaaga agacctaaaa
      301 tgggtcgact ttacgatctg tatgcgaaat gccggattat cgatcgagtc tttgattgaa
      361 tatattcgcc tatctagcga aggagaagca actgtatttg agcggcgtaa gcttttgatt
      421 gaagaaagtg aacttttgac gaaagaaatt gctgaaatgc aagagtgttt ggaacgttta
      481 caaggaaaaa tcaagaaata tgatcgtgtg ttatctagaa ataaaatgga atgtatcaag
      541 taaaaaaatc aaggagcatc gctatggagt tacgcttact aaaatatttt tggacaattg
      601 cagaagaagg aacaatctct caagccgcag aagttctaca tcttacacaa ccaaccttaa
      661 gccgacaact gaaggagtta gaagaggaac taggaacaga actgtttgta cgcgaaaacc
      721 gcaaaatgat cttgacagaa gcaggttatt ttctaaaaaa gccgagcaga agaaatttta
      781 gatttaacga atcaaaccac tcaagaattc gagaacagaa aaaaacaatt attcagcggc
      841 cgatttacga tcggctgtgt ggaagctgat aattcgaata cattggcact attactagaa
      901 aagttgatcc gtgattatcc cagcgttcaa tttcatatcc acagcggaac aagtgatgat
      961 atcacggagt gactagaaaa aagcctgtta gatttagcga ttttgctaga tccagtagat
     1021 acagagaaat acgaaacgat tttgttacca agaaaggaaa aatggggatt gctcatttta
     1081 gaaaattctg ttttagcaaa aaaagacgtg ataacaaaag aagactttgc gaatgtacct
     1141 cttttgatct caaagcgtcc agaaatacag cgaatgtttg ctgactggtt aggcataaaa
     1201 aaggagcagt tgctgatcac aggaacgtac aatttgattt tcaatgtatt tgcgctaatt
     1261 gagaatcagg taggtgctgc tttagtcata gaaggtgctg taagtcatct ttcaaagcga
     1321 gtgacattcg ttccgcttga cccaatgatt gaaacgaact gcatgctagt ctggaaaaag
     1381 aatcggattt tatcacctgt agtcaatgaa ttgattcacc gtttcaaaga agcttttgaa
     1441 cagtaataag tatctctaat cgaagaaatt tcaatacaaa agcgagacgt atgacagtct
     1501 tgcttttgta ttttttcatt tttaaatcaa aaaaaataaa gagatttaca taacttgtta
     1561 aataagcgct tacaaacata agaagaacgg aaatgaatca gctataataa ccgttgttct
     1621 ggattgtaat cattacaaag aaatgaagat tataactttg gaggtgttcg aaatgaaaga
     1681 aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc aatggttggg tatgattctg
     1741 ctgattgtat tagctttatt gacgatcatt ccttttttat ggatggtttc tgcttcattc
     1801 aaagcaaata atgaagtgtt cacgattccg attcaatgga tccctaaaac ttggcattta
     1861 gagaattatc tggttatttg gtctcgtatt cctttagtga cattttttaa gaatacagct
     1921 tttttaagcg ttgtgatcac gttgattcag ttgtttactt ctagttttgc tgcttacggg
     1981 ttttctaaac taaattttaa agggcgcgat attttatttt tagcatatat cggcacgatt
     2041 gctgtccctt ggcagtctta tatgatcccg cagttcatta tgatgagaaa agtgggttta
     2101 acatatacgt tgtggtcgtt ggtgctatta caagcgttca atgcttttgg tgtgttcttg
     2161 ttgaagcaat actacagtag tatcccagat tctttatgtg agagtgcgcg gatcgatggc
     2221 ttatcagagt ggggaatcta tcgcaagatc attttacctc ttacaaaacc ggcgattgcc
     2281 agtttgacga tcatcacatt cgttaataca tggaacgatt atatggggcc attcatttat
     2341 ttgtcttcta cggaaaataa aacaatccag ttagggttga aaatgtttat tgggctattt
     2401 gatgcagagt acgcattaat catggctgcc tcagttgttt ctgttcttcc tgttgctttt
     2461 atatttttag cgatgcaaaa atattttgta gaagggatcg cttcatcagg aatgaaggaa
     2521 taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg tttatatgga attcttttat
     2581 tggttgtatc aatcattggc aagtagcctt tgtttttggt gtgtcaatat cttgtatata
     2641 gcagctgcgt tccttttgtc gatagatgga aggaatgtct tttcctttat tttttcttta
     2701 ctttttattg gtccaggttg catcgtgatg ataggtatga cagaagaatt agcaaaagaa
     2761 aaaagttttt caccagtacg tttgttcata aagacgtttc gccaattttg gctaaaggga
     2821 tttttttatt ggctatttgc ttggatagtt agtgtgataa tgatttttga ttgtttcttt
     2881 tttatacgtt tttcttatgg aaaatggttg attccgttgt ttgttttact tgcttgttta
     2941 tcagtttcct ttagcatcaa ctactggtat tttcaggtga gaaatccagc gtcgaaacca
     3001 aatcaagttc tacggatagc gttctactat acgttgaaaa aatggtatgt gagtttacta
     3061 gattttttac tattgacaag tttgtttctc ttctttttcg taaagcccca atggtgtatt
     3121 ctactaggtc cttctatcgt atttggtttg atttacttca acaatcgtaa attgatgaga
     3181 actatggatc tgtagacttt ataaattgaa actttcaatt aagaatgaca gtttttatgt
     3241 aaaaactgtc attctttttc attttttgaa acatatggct aaacaaatga aataaaacgt
     3301 gctatacttt tgaatgaaac aggtttagaa actgtatcaa ttatttgtga ttatttctaa
     3361 ctaataatta aaaataaagc ataaaaagga cgatgttcaa tggacgaaaa aatgaaacaa
     3421 ggttcgaaat ctgtagtaga aagtttgatt aatcatcatg ttgattatgt attcggtata
     3481 ccaggtgcaa aaatcgatgg tgtatttaat gaattagagg atcaaggtcc tgaattaatc
     3541 gtgactcgtc acgaacaaaa tgctgcattt atggcacaag caatcggacg tattacagga
     3601 gagccaggag tagttatcgc aacaagtggt cctggtgcta gtaacttagc tacaggtttg
     3661 gtaacagcga cagcagaagg tgacccagtt ttagcaattg ccggacaagt aaaacgaagc
     3721 gatctattga agttgaccca tcaaagcatg gataatgcag cactgttcca gccaatcaca
     3781 aaatacagtg cagaaatcca agatccagaa acaatttcag aagtgattgc caatgcttat
     3841 cgtatggcta agagttcaaa aaaaggcgcg agctttatca gtatcccgca agatgtagtg
     3901 gatgcacctg tccaagggaa tgtcatcaag ccattgagcg atcctaaatt aggttctgct
     3961 tctgcagatg atatccgtta cctagctgaa cgcattagag aagcaaaact accggtacta
     4021 ttagtaggta tgcgaggatc aagtgaaaaa gaaacgttag ccatcaggca attagttgga
     4081 aagacagcat tgcctgttgt agagacattc caagcagctg gagtgatctc acgtgaatta
     4141 gaagcgcatt tctttggtcg tgtgggttta tttagaaacc aaccaggaga tatgcttttg
     4201 aaacgaagtg atctagttat tgctattggc tatgatccaa tcgaatatga agcaagaaac
     4261 tggaatgcgg aaaaagatgc gcggatcatt gtgatcgatg aagcgccagc tgaaatcgat
     4321 ccttttatgc agcctgaaag agaattgatc ggtgatatct cagccactct tgatttattg
     4381 acaggttcat tagagcctca acaagtgtca gaagatgcaa aagaatattt agcatctctt
     4441 caagcagaat taacggagcg ggacattgtt caatcaaaag gagaagcagg tatcttgcat
     4501 cctttagaag ttattaacac gcttcagtca aaagtaacag atgatatgac tgttaccgta
     4561 gatgtaggta gtcattatat ttggatggca cgccacttta gaagttatga acctcgtcat
     4621 ttgttattca gtaatggtat gcaaacgctt ggtgttgcat tgccatgggc gatttctgct
     4681 gcattggtgc gtccaaatac acaaatcgta tcagtttccg gcgatggggg atttttattc
     4741 tctgcccagg atttagaaac ggcggtacgt aaaaaattga atatcgttca tttgatttgg
     4801 aatgatggac actacaatat ggtagagttc caagaaaaaa tgaaatatca gcgggcgtca
     4861 ggtgtagact ttggtccagt tgattttgtg aaatatgcag aagcttttgg cgctaaagga
     4921 atccgagcta caagtgtaga ggaattagaa aaagctttag aagaaggatt tgcgacagaa
     4981 ggacccgtaa ttattgatat tccgattgat taccgtgata atgagaaatt gggagaaaca
     5041 atcttaccag atcaattcta ttaaggaagt gtcgacatga ctgaaaaaat cttataccag
     5101 cacgggacat taggagcttt gatggctggc ttgatggatg gaacagaaac gattgctcat
     5161 atattagaaa aaggaactct gggattggga acattgcatg ggcttgatgg tgaagtcatc
     5221 tttttagatg gcgtagctta tcaaggaaga tcagatggct cagttgccca attagatgga
     5281 agtgaactta ccccctatgc agcaattaca gattttacgc cagatactag tttttctgtc
     5341 tcaaaaactg cagatggaga acaattgaaa aaagacatat tagtaagaga agccggggaa
     5401 aacctatttt tggcagtgaa gataactgga ttattcaaaa atatgcacat ccgtatcatg
     5461 ccgaagcaag aaaaacctta cagaagacta gcaaaaattt cagagtctca accagaattc
     5521 caacaaagca atgtccaagg aacattagtc gggttcttta cgcctgaatt attccaagga
     5581 gttgctgcag cgggatttca cttgcatttt atcgatgata cacatacatt tggcggacat
     5641 gtaatggatt ttgaagtggc tgaaggaaaa gttgagatta gccgcatctc gtcccttgta
     5701 cagcattttc cagttgatga tccgactttt gttcacgaaa cgattgatta tgctgattta
     5761 gcagcagaaa tccaacaagc agaatagaaa aagacagcga gatgcgttca aggtgattaa
     5821 cctgacgtat ctcgtttttt tgaggaggaa acctagcgtt tacaaaaaaa ctgagctata
     5881 atcttctcca tagatcaaaa aggagcggtt tttatcatgt ggacagaaaa acagaaaaaa
     5941 gatgcatacg aaattttatt attacagcaa caaggattat tagaagcaga agataactgg
     6001 ttagcaaatt tagcaaattc atctgcattg ttgaatgaga ctttgcctga gactgtcttt
     6061 gcaggatatt atttattcga tggtaatgaa ttgattttag gaccgttcca agggcgagtt
     6121 tcttgcacac gtatcaagat gggaaaagga gtttgtggag aatcagctga aaaacaagaa
     6181 acattgatcg ttgatgatgt caaaacccat gcaaattaca tttcctgcga ttcagccgca
     6241 caatcagaaa tcgtagtgcc aatggtaaaa gaaggtaatt taatcggtgt tttagatatc
     6301 gattgcggag taacgaaagg gtatgatgaa atcgatcaag catttctaga aaaatatgta
     6361 gatcttttat tagcaaattt taatataaat gctttttcat ttattaataa ctaaaatcgt
     6421 ctattgaaac aaaaaagaag agaaatcaag aataaaaaga gccggaaaga cagtgggcat
     6481 ttctgttttt cggttttttt at
//
LOCUS       DBKOIJ010000147         6402 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000147 DBKOIJ010000000
VERSION     DBKOIJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.150"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(180..368)
                     /gene="rpmB"
                     /locus_tag="ACV5NJ_002359"
     CDS             complement(180..368)
                     /gene="rpmB"
                     /locus_tag="ACV5NJ_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HHY6486399.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            638..1000
                     /locus_tag="ACV5NJ_002360"
     CDS             638..1000
                     /locus_tag="ACV5NJ_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HHY6486400.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1052..2734
                     /locus_tag="ACV5NJ_002361"
     CDS             1052..2734
                     /locus_tag="ACV5NJ_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HHY6486401.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2851..4887
                     /gene="recG"
                     /locus_tag="ACV5NJ_002362"
     CDS             2851..4887
                     /gene="recG"
                     /locus_tag="ACV5NJ_002362"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HHY6486402.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4936..5937
                     /gene="plsX"
                     /locus_tag="ACV5NJ_002363"
     CDS             4936..5937
                     /gene="plsX"
                     /locus_tag="ACV5NJ_002363"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HHY6486403.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6059..6301
                     /gene="acpP"
                     /locus_tag="ACV5NJ_002364"
     CDS             6059..6301
                     /gene="acpP"
                     /locus_tag="ACV5NJ_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HHY6486404.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2145 a         1109 c         1442 g         1706 t
ORIGIN      
        1 ctctgcttaa aaagagtcta gttttcacaa aaaaataaaa tttagaataa ataacttcat
       61 aatttgttcg tataacaaaa ataaacgcaa aaaaagggta ggaatcaaaa gtcccaccct
      121 taaaaatttt tattaaataa aaaaccgttc actatctgaa cggtccaagt atcattcaat
      181 taaacgcgtt caacttttcc tgatttcaaa gcacgagttg acacccaaac ttttttaggt
      241 tttccgtcga ccataacgcg aactttttgt aagttaggtt taacagtacg ttttgttgag
      301 ttcatagcgt gtgaacggtt gtttccactt tttgtcttgc gacctgtaaa gtaacaaact
      361 tttgccatgg taatttcctc ctttgccttg atttggagca atagaataat tcagctcata
      421 ccagactaac atatcatata gaatgggaat ttgcaagtac attttcatag atacaattga
      481 caattgagaa gaataattgt aaattaaaca aggttatacc tgaaattttg caaggttttt
      541 gagtactttg ggtaactgcc ttttatttca agttgcgcta tgataaaata tgatagaaga
      601 aaaatgggag ttttttaccc gttaaggagg ctcattcatg gctgtaaaaa ttcaaacgtc
      661 agctggaacc attgaaatta ccaatgatgt aattgcaaca gttgttggtg gcgccgcgac
      721 agatgtgttc ggtgttgtag gaatggccag caaaaatcaa attaaagata atattactga
      781 gattttgcgt aaagaaaatt actcaagagg agtagttgtc cgccaagaag ataatggtat
      841 tgctgtagat gtttatacga ttatcagcta tggtacgaaa atctctgaag tttcccgcaa
      901 cgtccaagaa aaagtgaaat acaatctaga ggcattatta ggcgtaactg ccaattcagt
      961 taatgtcttt gtacaaggtg ttcgtgtact gccagactag gcaggagact ggctggtaac
     1021 tcagcagtcg atctgaagga ggattatcta ggtgagtgta acagaaataa gtgcaagcca
     1081 gttccaggaa atggtccagg caggtgcgaa tcgtctgcaa gtaaatgcag aatacgtcaa
     1141 ttcattaaat gtattccctg tgccagatgg cgatacggga acaaatatga atctatctat
     1201 gactagtgga gctaaggcag tagtagattc aacctcagaa aaagtaggag aattagcagc
     1261 aatgctttca aaaggattgc tgatgggtgc gcgcggaaat tccggcgtta ttttgtctca
     1321 attgtttcgt ggattttcta agcaaattcc agatgttgtc tcattaaatg cttctgatct
     1381 tgctgcagcc ttcactcatg gagttgaaac tgcgtataaa gcagtaatga aaccagtaga
     1441 aggaacgatt ttgacagtag ctagagaagc agcgaaagct ggagaaaaga aagcaaaaag
     1501 tactgatgat gtcgttgaag taatgacagc tgttgtaaaa ggagcaaagc gtgctttagc
     1561 taaaactcca gacttgcttc cagtcctaaa agaagtcggt gtcgtagaca gtggagggca
     1621 aggactgctg tttgtttatg agggcttttt aagtgcattg aatggagaat tccaagcgga
     1681 tgaaacatat gaaccttctc cagcagaaat ggatgatatg gtcaatgcag aacatcatcg
     1741 cagtgtccaa ggtcaaatgg cgacagaaga tatccatttt ggttactgta cagaaatcat
     1801 ggtaaaaatc ggagaaggac ctacagtcga tagtacattc gattacgaag aattccgtaa
     1861 ctatttagat ggtataggag attctttact tgtggtcaat gatgacgaga tcatcaaagt
     1921 ccacgtgcat acagaaaacc caggagaagt cttgaattat ggacaaaaat tcggttcatt
     1981 agtcaaaatc aaagtcgaca acatgcgctt gcagcatgag acgattttgg aacacgatga
     2041 agaagtgaac gagtttgcga aaaaagaaat accacgtatc ccgtatggta tcgtagcgat
     2101 cgctgctggt aaaggcgtcc aagagctgtt tgaaagttta ggcgctaatt atgttatcag
     2161 tggtggacaa acaatgaatc caagtacgga agacattgtt aaagcgattc aagaagtaaa
     2221 cgcagataaa gtgattgtct tgccaaacaa taagaacatc ttcatggcag ctgatcaagc
     2281 agcagaagta gcagatatcc cagtagcagt tgttccttca agaacgattt cccaaggatt
     2341 gacagctatg ctttcattca atgatcaagc ttctttagaa gaaaacaaag aaacgatgac
     2401 agaaatgttg tctagcgttg tgagcggtca agtcactcac gcgatcagag atacctcaat
     2461 tgacggtgta gagatcaaag aaggagactt tttgggcatg atcgatggaa aaatcctgat
     2521 ctctaaccca gatatcacag caacagcgtt aagtacacta gaacaaatga tcaatgagga
     2581 cacagagatc gtaacgatct tggcaggtga tgaaggaaca gttgaacaag caaatgaatt
     2641 tgctgagaaa ctaacagaaa aacatcctga tttggaaatc gagatccacc aaggagatca
     2701 gcctgtctat ccttatctat tttctgctga ataatttctg tgtcagaata tctttctaac
     2761 acagtattca ttcataaaag gccttcggtt agtgatcagg tatgatcgct gaccaagtcc
     2821 tttttttgtt aaaggaggga ggaaaaattt atgcgttctt tagatgatcc aatcactatg
     2881 ctggcaggag tcggagaaaa aagagcagct agtcttgcca gcttgggcat ccaaacgatt
     2941 gaagacttac tgacttatta tccttttcgc tacgaagata ttcaggaaag aaatctaaac
     3001 gagatccagg atcaagaaaa ggttactttg aaaggaatcg tagtctcgcc tcctgtgatg
     3061 aatcgatttg gttataaaaa aagccgattg caatttcgta tgatgcaaga acatgatgtg
     3121 ttcaacgttt ctttttttaa tcaaccttat ctaaaagaaa aagtcatttt atcggaggaa
     3181 atcgccgtat acggtaaatg ggatgcaaag cgtaaagcct tgaacgggat gaagatttta
     3241 ggttctcaaa caatggatga cttttcgccg atatatcacg tcaacaaatc aattcgtcaa
     3301 acgacattag ttgatctaat caaaaaaggg tttcaagaat acggcacatt gattgaagaa
     3361 aatctgccaa acaaccttgt ggagaaatat cgactgcttg accgagcttc tgctgtaaga
     3421 agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt
     3481 gaagagttct ttttatttca aatgcgtctt caaggattga aaaaagcaga aaaagcagaa
     3541 acgaatggtt tggagatcct ttatgatgtc caaaaattga aacaatttac tcaaaagcta
     3601 ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc
     3661 agtccgcagc atatgcagcg acttttgcag ggagatgtag gtagcggaaa aacagtggtt
     3721 gccgcgattg cgttatacgc taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct
     3781 acagaaatct tggcacaaca acatatggaa agcttgcaac agctgtttga tcccaatgaa
     3841 gtaaccatcg cattgattac ggggtcaacg aaagcaaaag aacgacgtga gctccttgaa
     3901 aaactagagc aaggggaaat taacgtcgtg atcggcacac atgcattgat ccaagatgga
     3961 gtcgaatttc agaatttagg tctagttatt acggatgagc agcaccgttt tggcgtgaac
     4021 cagcggaaag tacttcgtga aaaaggctgg cgaccagatg tcttatttat gacagcgact
     4081 ccgattccta gaacattagc tattacagca tatggtgaga tggatgtatc tatcattgat
     4141 gaacttccag ctggacgtat cccgatcgaa acacgctggg taagaacacc tcaattggac
     4201 agtgtactgg attggacgaa aaaagaactg gcaagaggac atcaaatgta tgtcatctgt
     4261 ccgctgattg aagaatcaga agcattagat gtaaaaaatg cagtggaaat ctatgaaaaa
     4321 cttcgtgaac tatttgcacc aaaatatgaa gttggcttgt tacatggtaa aatgaagaat
     4381 caggaaaaag aagtaatcat ggaagccttc aaagacaacc agatgcagat attagtttcg
     4441 actactgtaa ttgaagtagg ggtaaacgtg ccgaacgcga ccgtcatgct gatcatggat
     4501 gcggatcgat ttggattggc acagctgcac caacttcgtg gacgtgttgg acgcggaagt
     4561 gatgcttcct actgtatctt agttgctaat cctaaaaatg aactaggtgt ggaacggatg
     4621 aaaatcatga cagagaccac caatggtttt gtactaagcg aaaaagatct ggaattacgt
     4681 ggtcctggtg aagtatttgg cttccgtcaa tctggtctgc ctcaatttgc gattgctgac
     4741 ttggtgacgg atggaaatgt tttggaggta gccagagaag aggctgcagc cctttggcaa
     4801 ataaaggatt ggcagatgct tcctgagtat cagcctttag cgcaaagcct ccaaatagaa
     4861 aatcaggata atcgttattt cgattagcag actgtgctat actttatact gatgacggaa
     4921 aaaggagtgg aaacaatgaa aattgccgta gatgcaatgg gcggagatca cgcacctcaa
     4981 gcaattgtag aaggcgtggc tttagcgcag caagattttc cagaagtaga attcttactt
     5041 tacggaaaag aagcagaaat caagaaatac ttaacaaatg aaaaaaacat cacgattatc
     5101 catacagacg aaaaaatcaa tagtgatgat gagccagtaa aagcaattcg acgaaaaaag
     5161 actgcatcaa tggtccttgc tgcacaagca gtgaaaaacg gtgaagcaga tgctgtattt
     5221 tcagcaggga atacaggcgc gctactagca gcagggttgt tcatcgtagg acgtatcaaa
     5281 aatatcgaac gccctggctt gatgtctacc ttgccagtag tagggaaaga aggtgccggt
     5341 ttcgatatgc tggatcttgg cgctaacgca gaaaacaaac cagaacattt gttgcaatac
     5401 ggtattttag gttcttttta tgcacgtaaa gtccgaggta tcggacgtcc tcgtgtcgca
     5461 ctcttaaaca acggaacaga agcaacaaaa ggtagtgaag tgacgaaaaa agcctatgaa
     5521 ctccttcaga atgaaacaag cttgaatttt attggaaacg tagaagctcg tgagctactc
     5581 aatggtgtgg ctgatgtagt ggtcacagat ggatttacag gcaacgctgt tttgaaatct
     5641 attgaaggaa cagcaatgaa tatgatgaat ctattaaaat ctgctattct aaatgaaggg
     5701 atcaaaggaa aaatgggcgc tatgctttta aaagacggct tacgttcttt gaaagcagaa
     5761 atggattatt ccaaacatgg cggtgccgtt ttgtttggct tgaaagcacc agtcattaaa
     5821 actcatggag caacaggccc cgatgctgtt cgctatacga tccgccaaat ccacacaatg
     5881 cttgagacag atgtagttgg acaattagtc gaacaatttg aaaaaagcga agaatagtat
     5941 ttggatatct gaagcgatcc gttccattga aaaaaaggtg gacattcctg aaaaatgcca
     6001 gtaaaattag ccatggtgta ggtaattgac aagtgtatga ttcttggagg tgaaaaagtt
     6061 gactcgcgaa gaagtattta acaaagtagc gaaaattatt tcaaatcatt ttgagataga
     6121 taccgataaa gtgacggatg aattgaacat caaagatgat ttaaaagctg attcaattag
     6181 tattatggaa ttcgttttag agcttgaaga tgaattcggt acggaaattt cagatgaaga
     6241 cgctgaacaa attgaaacag tcggtggcgc agtagattat atcagcagcc atcttaaata
     6301 atttcaaata aaagcaaaga aggataaaaa gctgcatgca gctttttatc cttctttttt
     6361 taatcttttt gacacgtaaa agtcattttt ctgataattc tg
//
LOCUS       DBKOIJ010000148         6384 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000148 DBKOIJ010000000
VERSION     DBKOIJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.151"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            112..450
                     /locus_tag="ACV5NJ_002365"
     CDS             112..450
                     /locus_tag="ACV5NJ_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486405.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            536..781
                     /locus_tag="ACV5NJ_002366"
     CDS             536..781
                     /locus_tag="ACV5NJ_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486406.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            801..2957
                     /locus_tag="ACV5NJ_002367"
     CDS             801..2957
                     /locus_tag="ACV5NJ_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486407.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            2969..3202
                     /locus_tag="ACV5NJ_002368"
     CDS             2969..3202
                     /locus_tag="ACV5NJ_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHY6486408.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3192..4058
                     /locus_tag="ACV5NJ_002369"
     CDS             3192..4058
                     /locus_tag="ACV5NJ_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486409.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4073..6043
                     /locus_tag="ACV5NJ_002370"
     CDS             4073..6043
                     /locus_tag="ACV5NJ_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486410.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         2454 a          876 c         1249 g         1805 t
ORIGIN      
        1 gatgtaaata tcgagacgtc tttttcttag ttagatcatt gacaagtgaa caaataagat
       61 ttgtcgatga tataattaac ttattaaaaa tattaaaaag gaggttttta tatgttcgat
      121 tctattaaaa atttggttag cggaatgacg cctgatatga aaactttagt acaagttgtt
      181 ttgtatttct tggcattcgt tttttcgttg aaaccaggat tcactgcaat gaaatttttt
      241 ggtgatgcaa aatggggaga tggattaaaa tatgttgggg cgattattgc aatctttatt
      301 attcctgtaa ttacaaccgt tatgctttac acaatgggta ctaaaacagg taatgatttg
      361 aaccaaaaag ctaatatcat atcagcactt attccagttg ttggtatcta tctagtttca
      421 aaatttccaa aaaatgaact gttaggttaa aagtgaattg aacaaatttg gataatatgc
      481 cgatttgttc aatttacttt ttaccaaaaa actacgctcc aagcgtagtt tagaagtgag
      541 ggaagaaagt aaatgtaaga tggtttttga tagagaaaaa atgttagctc acgccaacga
      601 agtattaatg agcagtctga aaggtaccga actggcaaag ataatgaata tgaatgttaa
      661 ccagttctat gattatcgaa atgggagtaa aaagattgaa aaagccagac ttgaaacgct
      721 tattaaattc gaaaaagcat atgtatatat gctagataaa cagaaaagaa ctattgactg
      781 aaaaaaggag ttttacaata atggcaaaat tcaaagatat taccgaatct attaattatg
      841 tggcagaaac agttcctgta gttgcactct ctttgaagcc acaattactc gaagtgtaca
      901 aaaaagaaga aaaagaagcg aaggcaaaaa aagctaaagc aaaggtatgg aaagaattcg
      961 atgcgttgct gaccaaagat ttaccaaaga aaaattatat ggtaattgaa gatgtacaaa
     1021 agctcaacag tgagggagtt atagatgagg atgatgctat tctcgattct gagaagtatg
     1081 tcgttctatt gaagtcaagt ttgaattatg tagctgatct accagaaaaa tttaaagaga
     1141 agctattagc aggtgtccgt tttatggata gtagtggtca attattagtt attccggatg
     1201 atcctaatgt gtttgaatca gaagttgaag aagctacaga cttctatagc gcagtagagc
     1261 aattagaagt agctactaat ggattagaac gaattgctga gaccttcaac tcttctgaag
     1321 aagttgaatt tgaagccgta gcagaaaatg taccagatga aaaagaagct ctttttgtgg
     1381 atgataacaa accaaccgaa gcagaaaacc aagaagtaag catagaagaa acacaaatac
     1441 aaacagaaac ctaccaacaa ccaaaaattg atgaaaaacc cgaatcccaa agtgacaaag
     1501 aaaattatgt ttatgaagct gaaaaaatca catctgaaag tgatgtaccg aaaaacttag
     1561 atgctgattt tttagaaaaa aattttgaac agatcattgc tgacaaattt caacctgtag
     1621 ttttagatga gattaatgac cctgtcgatt taaacgagga gatgctatcc atacctgagt
     1681 tgaaaccatt gctgttttta gctaaagaga gtatgaacgc tcgcatctct attgctaata
     1741 ataaaatcaa cagtgaatta aataagacga aggagcaatt gatcctcaaa ggaaagcaaa
     1801 tgttagctga tgagcttttg cggatagagg aagcaacctc attaaatggt caaaatcagt
     1861 ttacaaatac actatcggtt gctaaagaga gctacgaagc agaagcatct aaaattgatg
     1921 atttagtgat ggaaaaatcc aatatcattc gtaaagatta tgatatggcg aaacaggctt
     1981 atttggatgc tgaactaaag aaactagagc aacggtatga tgctgagcat ttaccagaag
     2041 ttaaacaaca agaagatgaa tatagaaaag caactgaaga aagtcttgat caacagtacc
     2101 aagaagcaat tgaatctatt aaaaacgatg cattgaaaca aagccaagaa gcaaaagaag
     2161 ttgtggtgga acgaattatc aattcattga aaccagaaat tgaaaaatca ttaacagacg
     2221 tgcaagaaaa aactttagag attgtcgaaa aaacaaagtc agaaaatgaa gctgagttcc
     2281 atgatacaaa agagaaaaca acagatgtgg ttgtcaaata ttttgcgaac aaagaagaag
     2341 ttgaaaagca ggtagctaac aacacgaaag aattagaaac aaaacttgta gagcaacaaa
     2401 aaactatcga agaatataaa gatcgaatga aagaggtcaa agatttagca gagggttata
     2461 aattaaatag cgaaaagttg gcagaacaaa gtgaagcttt aagagaagac ttatctcgtt
     2521 cccgaagtga tctagctcaa atggcagaaa aaacgaaatc attagcagtt gctacagaaa
     2581 atcaatcaag ttcaaaacgt gattttacaa gtaaaaagat ggctactatc atttctgtcg
     2641 ctgcaatggc ttgcggaacg atcttgggat cgtcctactt tattagtcat aataatcaac
     2701 aagcacaggc tcaaactcaa acatctattt cgagagtgga aaattcgatt gggacaaata
     2761 cgaactctgc ttcaacaaat agtagctatg aagcaacacc aactacagag ttcaaagttg
     2821 ggaaagatgt tgtcatcagt attgatggga aaacaactaa ggctgaagtg atttctattg
     2881 atggagaaaa agcagttgtg aaaactccag acggaaatca gtacaaaatt caagctcact
     2941 tatctgataa taactaagga gggctattat gcgagaagaa aaaatagttt atccagtaat
     3001 tcaaaacaat caagaaaaca tgacctcttc tcagcactta caggaagcac agctcgtgta
     3061 tcaggtaaat gaactaccac aaacgaacga gacaatgagc aagtcaatat ctttaattgg
     3121 cttgcttatt gttgcaatag tgataactgt aggtgtttac cgtaagttga acgtgaaagg
     3181 aagtatttct gatggcagat aactcaaact atggatttaa acataactta catcaaaaac
     3241 cttctgaaac aattgtcaga gttgaatggc ttgaaaagca tgtcaatagc acctattctg
     3301 ttacagcaaa aagattaatt gtatcagcta tagttgttgc agtcgttgtt cttggatcaa
     3361 ttgtttactt agtttctgat acgattaaac tgaaatttat ttttgttcca acgttattaa
     3421 tgataattgg atttcttatt ctaattagcc agtatatgtt tattgatcca tctagtggtt
     3481 cattgatttc tggttctatt agactgttca aatcagtagt taattcagca agagtatcta
     3541 tggggaaaaa gaaaccattt cgaggcacag agattttaaa agtgaaacaa gatggaacaa
     3601 tcgtatatcg agatgggagt gtagcattaa tgttcaatct agatggggct acatcactaa
     3661 ccgcttttcc agaagaagtt atggctcagg aatcgaaatc aatacaatat caaaatacca
     3721 gagatcgttc cttagctgaa atcaaaatca cttcatctca acctcagaat acggaaagac
     3781 aaactcgaaa tatgaaaata caaaaacgaa atgcaaggag taacaaagca gtctttgaca
     3841 taatagatca gcaagaacga tttgtatcag aatatattga tggtgtgaaa acaacagtaa
     3901 tccaatatct tatcttgaaa tctcaatcta agatggtatt ggatgaaggt gtggaacgtt
     3961 tagaaaacta tacgagtaga ggactttatt atagtgtcac tcagttgtca gctaaacaaa
     4021 caaaagaaat attatctgat atttatgcac tgaaatagag gtgagagatt aaatggaaat
     4081 tatgggtaga tcacaactat taaatttcaa aaaaaagaga aaaactaaaa aagaacaaaa
     4141 aactagaaat tcaaaggtag tgaataatac ctttgttagt gatttaacat ctttagttgg
     4201 ttatcaattt acaccatctt acattaggac aggtaagcgg tggggaacaa ttgtgaaatt
     4261 agtgaatact tatggaatga atagaaatta tggatttggt tggatggtta aagctattcc
     4321 tgaagtgaga gtagaagggg taaaagccta tctaattgaa gcagataagc cagtgtcaca
     4381 aaaagatcaa caagaaataa tgcacgagaa tgttggggga tcaacgaata gttatgaaaa
     4441 taatttagaa ggaaaaaatt cagatgctga aattagcaaa actgttatgc ggattatgga
     4501 tttgaataca gcatctttac tagacagcat ggcaaataaa attattgatt ggaagctaga
     4561 aatcctatta gtatccgatt ctcctaataa aatcagcgaa cagctaatta aactccaacg
     4621 gttgtatgat gaaaaaatgt atggattaaa gcttatgtca tatggaggag atcaacgtga
     4681 tctgtttgat agacttttaa gtgaaccaat cgggaatgtc tatgagtata cgtctatgag
     4741 ttcggatttt gcaggaaatg atcatgcaat tcgcaaaggt ttagatgatg cgaatggatg
     4801 ggctatcggc actatgtcta ctagctacgc aagtggtgta gcttttatgg cactggatga
     4861 ttcttttaca agtaaagttt tagtggcatc acacaaaaac gctacaatta aaggctatga
     4921 tgaaaaaatc tcagcatcat cactttgggg gcaattgatt gctaataatg caatggcaaa
     4981 tcataaacga acttttcata ttgtcttaaa taaatttgat tactttggtg atccaactgg
     5041 tagaacattt gttgccaaac cttcgttaaa cagtgtaata gaaaaggtta atttggctga
     5101 agaaggtctt aactctttgg agctttttgg taatcaaaag gacattggtg atttatatag
     5161 acggaatctc gataaactaa ctcagatttt ttatttgtta gcaggcagaa cattagattc
     5221 tgcaactaga gttttattga aaaaagaatt aggtaacttt tatataagtc agaaactttg
     5281 ggatattaat tatgaagaac gttcaaaact tttggtgaac agtagtgata caatgccagt
     5341 tcttggggat tttattagct atttgactaa tgctgaaaca aaagccaaaa aatctggtac
     5401 tgaaaaagaa gtcgatcgta ttattgtatt aaaagacaca ttgagaacag cattagacag
     5461 cttctctagg atgtttaatc aaagaacgac actgaaagaa atcggtaaaa gtgacaaatt
     5521 acagttttac tataacttag gtgatctaag agcagaaacc aatgttatgg aagctcaatt
     5581 tttaaatacc tttgatttca tcacatcaca agcaaaaaaa gatgatattg ttatgattca
     5641 cggcttcgat aagttatcta ttgaaaccgt tgggctaatt aaaaaaagat taacaatgct
     5701 tgaagaagat ggaattcgtc ttgcctttct atttgataca atcggttcag gagaagacaa
     5761 tgaatacaaa gaagatattg aaacagctga tatattcaat acagatggaa aaatttacca
     5821 aagttttgaa agtgatttta attacactat cattggaaag atgaccagtg gagagttaat
     5881 gaagtatgaa aaaaaagttg ctcctgctag aaaaggagaa gatgatcaaa aactttctgt
     5941 tgatttgaga cagtatttga caacaggaag aagtaataaa tttcagatca ggagaccagt
     6001 ggatctgact aataattttg ttattggaga gtttattctt taagttttat ctttgatttt
     6061 gagtgtataa ggaaaggata agaaaaaaca tgtatatttg agactaaaaa cagtcttaga
     6121 gaacatggta cgttttgtga aaagagaact atagaaattt cataaagtga aaccttactg
     6181 agtgaaataa ttatagtgga ggttaaataa tgaaaccaaa cataaaaatt ttagatcgaa
     6241 tatttctagg tagagacaca gaagtaatcc taattcaaca tgaagaagga tttgaagtta
     6301 gtataggtat tcagaaactt caaaaacctc attattgtaa tcagttgtat aaaaacttta
     6361 ctgatgaaga aaaagcgaga gttt
//
LOCUS       DBKOIJ010000149         6382 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000149 DBKOIJ010000000
VERSION     DBKOIJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.152"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(144..722)
                     /gene="pgsA"
                     /locus_tag="ACV5NJ_002371"
     CDS             complement(144..722)
                     /gene="pgsA"
                     /locus_tag="ACV5NJ_002371"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HHY6486411.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(931..1854)
                     /locus_tag="ACV5NJ_002372"
     CDS             complement(931..1854)
                     /locus_tag="ACV5NJ_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HHY6486412.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(2593..3879)
                     /gene="yfmH"
                     /locus_tag="ACV5NJ_002373"
     CDS             complement(2593..3879)
                     /gene="yfmH"
                     /locus_tag="ACV5NJ_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HHY6486413.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(3872..5140)
                     /gene="yfmF"
                     /locus_tag="ACV5NJ_002374"
     CDS             complement(3872..5140)
                     /gene="yfmF"
                     /locus_tag="ACV5NJ_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmF"
                     /protein_id="HHY6486414.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5392..5853
                     /gene="mscL"
                     /locus_tag="ACV5NJ_002375"
     CDS             5392..5853
                     /gene="mscL"
                     /locus_tag="ACV5NJ_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HHY6486415.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5912..6358)
                     /locus_tag="ACV5NJ_002376"
     CDS             complement(5912..6358)
                     /locus_tag="ACV5NJ_002376"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6486416.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1790 a         1351 c         1135 g         2106 t
ORIGIN      
        1 ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata
       61 aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc
      121 acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg
      181 aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct
      241 actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc
      301 atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg
      361 acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc
      421 caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta
      481 tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt
      541 tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt
      601 gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca
      661 accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc
      721 acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat
      781 tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg
      841 aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa
      901 gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac
      961 attttttgga ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt
     1021 gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa
     1081 cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac
     1141 tagcacacct aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc
     1201 gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt
     1261 cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct
     1321 ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc
     1381 tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat
     1441 cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg
     1501 cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc
     1561 agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc
     1621 ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa
     1681 ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg
     1741 ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat
     1801 ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg
     1861 ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt
     1921 tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat
     1981 ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga
     2041 cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa
     2101 atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac
     2161 aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
     2221 aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
     2281 ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata
     2341 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
     2401 tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag
     2461 agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa
     2521 agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca
     2581 taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa
     2641 tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc
     2701 aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga
     2761 atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc
     2821 tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt
     2881 ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa
     2941 ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga
     3001 agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc
     3061 tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt
     3121 aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg
     3181 tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat
     3241 caatttctca ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa
     3301 cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc
     3361 cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat
     3421 ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt
     3481 gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt
     3541 cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc
     3601 attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa
     3661 caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc
     3721 atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt
     3781 gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata
     3841 aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa
     3901 agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc
     3961 tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca
     4021 aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca
     4081 gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta
     4141 attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga
     4201 cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt
     4261 tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga
     4321 ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca
     4381 gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt
     4441 gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg
     4501 cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg
     4561 ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga
     4621 ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg
     4681 cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt
     4741 tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata
     4801 aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac
     4861 cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata
     4921 atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccggat
     4981 aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt
     5041 gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta
     5101 agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac
     5161 agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc
     5221 ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt
     5281 atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta
     5341 ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa
     5401 gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt
     5461 ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata
     5521 ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt
     5581 gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt
     5641 acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag
     5701 aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat
     5761 cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta
     5821 gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga
     5881 ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt
     5941 cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact
     6001 cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt
     6061 tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt
     6121 cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt
     6181 aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt
     6241 catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga
     6301 cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta
     6361 agcaaacgcc ccctgctttt tt
//
LOCUS       DBKOIJ010000150         6312 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000150 DBKOIJ010000000
VERSION     DBKOIJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.153"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(99..1229)
                     /locus_tag="ACV5NJ_002377"
     CDS             complement(99..1229)
                     /locus_tag="ACV5NJ_002377"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HHY6486417.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACV5NJ_002378"
     CDS             complement(1878..3080)
                     /locus_tag="ACV5NJ_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6486418.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACV5NJ_002379"
     CDS             complement(3140..3343)
                     /locus_tag="ACV5NJ_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HHY6486419.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4540)
                     /locus_tag="ACV5NJ_002380"
     CDS             complement(3485..4540)
                     /locus_tag="ACV5NJ_002380"
                     /inference="COORDINATES: protein motif:HMM:NF014537.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HHY6486420.1"
                     /translation="MQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFEELD
                     YAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQFFRK
                     VLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNESNG
                     ETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKIISK
                     THDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSFEKT
                     MSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQEEV
                     EYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACV5NJ_002381"
     CDS             complement(4610..4717)
                     /locus_tag="ACV5NJ_002381"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486421.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACV5NJ_002382"
     CDS             4880..5692
                     /locus_tag="ACV5NJ_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486422.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACV5NJ_002383"
     CDS             complement(6066..6257)
                     /locus_tag="ACV5NJ_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486423.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1875 a         1297 c          996 g         2144 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt tt
//
LOCUS       DBKOIJ010000151         6172 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000151 DBKOIJ010000000
VERSION     DBKOIJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.154"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(63..389)
                     /locus_tag="ACV5NJ_002384"
     CDS             complement(63..389)
                     /locus_tag="ACV5NJ_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486424.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(435..797)
                     /locus_tag="ACV5NJ_002385"
     CDS             complement(435..797)
                     /locus_tag="ACV5NJ_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HHY6486425.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(834..1048)
                     /locus_tag="ACV5NJ_002386"
                     /pseudo
     CDS             complement(834..1048)
                     /locus_tag="ACV5NJ_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1176..1511
                     /locus_tag="ACV5NJ_002387"
     CDS             1176..1511
                     /locus_tag="ACV5NJ_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486426.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1587..3584)
                     /locus_tag="ACV5NJ_002388"
     CDS             complement(1587..3584)
                     /locus_tag="ACV5NJ_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486427.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3682..3957)
                     /locus_tag="ACV5NJ_002389"
     CDS             complement(3682..3957)
                     /locus_tag="ACV5NJ_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486428.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4189..4923
                     /locus_tag="ACV5NJ_002390"
     CDS             4189..4923
                     /locus_tag="ACV5NJ_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHY6486429.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            4962..6104
                     /locus_tag="ACV5NJ_002391"
     CDS             4962..6104
                     /locus_tag="ACV5NJ_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6486430.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2098 a         1135 c          890 g         2049 t
ORIGIN      
        1 ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat aatacattta
       61 aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg gtctttcttt
      121 ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc tttagcccgg
      181 tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg atttaagttg
      241 tactgttctt gtaaaatctg tgttaccaat ggtttcgatg acttctctaa tccagatgta
      301 cctgtcattt ttgaaaaaat gatacctata atagccaaga caacgataac tataatgata
      361 aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa aataatttgc
      421 tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat tgctccagct
      481 acccctgcta tcatcaaaat cgtacccatt tgttcatctt tatctcgata taagtagccc
      541 ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgctac taccatagaa
      601 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
      661 atttcattaa ttgtctgctc agtacttttc tttactgcgt ctgctccagt tgtctcatct
      721 gtttcttgta gtgctccgca aaactggcaa tacttacttt catctgttat ttctttgcca
      781 cattttctac aatacatgtc ttctcctcct aaaaacattc aattgtttat tagttaactg
      841 tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa tatctcagaa
      901 agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
      961 tgctgtgtaa cattcatttt tttgctaact ccgtttgaga aagtcctttt tcttttctgt
     1021 atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca
     1081 atatgatgta tttcaactca taaaatataa aaaaacttca aataccatta caaatacaac
     1141 aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa tatgcaaagg
     1201 tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct gaaaaaatct
     1261 tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct aacctcgata
     1321 gcttaaccgc tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg
     1381 tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact gaagatgcag
     1441 ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat
     1501 tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat
     1561 aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt tctcttcctt
     1621 actctccgtc catcatctct tgttacactt gtaaattgat aaagaggaac taattcaact
     1681 cctgataata tcaattcttt tattacttca tctatccctg ctccttttgc taggtagact
     1741 aaaaaactac tacttgcttt cacattgtat agtctcttaa tatccttaga agctcgttca
     1801 agagtgaatc cttgtttaac aaagtagcct ctgttttctt ttaaagcatt ttccacgctg
     1861 ttattcccaa cattacgtag acgttcacaa acagtgttag taagttcttt ctctgtcatt
     1921 agatatgttc cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat
     1981 tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt
     2041 tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaaatttt
     2101 tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg
     2161 tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt ctcttccttt
     2221 aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc
     2281 aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta
     2341 ccatagtaaa aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt
     2401 tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt
     2461 tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca
     2521 aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc
     2581 tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact
     2641 ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca
     2701 gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag
     2761 tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca
     2821 ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct
     2881 ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc
     2941 cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta
     3001 atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa
     3061 aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat
     3121 tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc
     3181 ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta
     3241 tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc atctttactc
     3301 aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact
     3361 ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat
     3421 gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct
     3481 aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt
     3541 tctttatcta cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta
     3601 taaaattctg ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat
     3661 gttttagtct tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga
     3721 ttttttaaat ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata
     3781 ttttctaatc gttcctactg atacacctag atattcactt gcctgctgat agtccatata
     3841 ctctagagtg actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc
     3901 tttcacttgt tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt
     3961 tccccctcct ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc
     4021 tcaaaataaa agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca
     4081 tcgacataca cataatacct cgataaaaaa gaggtgtcaa tatctttgta aaatttattt
     4141 acaacacccc tttttcgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt
     4201 ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga
     4261 aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt
     4321 gacaaaaaaa ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta
     4381 tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa
     4441 gaatatattt tgggaattat tctttataga gatagcattg tactagatgg tataacattt
     4501 ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta
     4561 gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac
     4621 aagacttgta ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa
     4681 gaattattca tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc
     4741 agtatagaag aactactaga aagaatggta gatgaatggc tacctattca attgaaagac
     4801 atgtcttatc ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa
     4861 acgatttcat caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc
     4921 tagtggtaag tactccttgt aaactaagag gagatgattt catggcaaaa atatcaaagt
     4981 acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac
     5041 gaactggtga aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa
     5101 aagattatga cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata
     5161 tgacatttga agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt
     5221 caacaatcgc aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc
     5281 ttaaactatc gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta
     5341 aatatgaaag ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg
     5401 caatgcaata tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt
     5461 atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta
     5521 aagattttgg taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga
     5581 gaaagagtga agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac
     5641 atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa
     5701 caagagcttc tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact
     5761 ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc
     5821 aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt
     5881 taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata
     5941 cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag
     6001 ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta
     6061 agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat
     6121 ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat at
//
LOCUS       DBKOIJ010000152         6125 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000152 DBKOIJ010000000
VERSION     DBKOIJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.155"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..861
                     /locus_tag="ACV5NJ_002392"
     CDS             <1..861
                     /locus_tag="ACV5NJ_002392"
                     /inference="COORDINATES: protein motif:HMM:NF037736.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMODS domain-containing nucleotidyltransferase"
                     /protein_id="HHY6486431.1"
                     /translation="FMEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRA
                     INREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLL
                     QVIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNM
                     GGNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYE
                     AMGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIIC
                     LEKVLKKIAL"
     gene            881..1441
                     /locus_tag="ACV5NJ_002393"
     CDS             881..1441
                     /locus_tag="ACV5NJ_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HHY6486432.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2330..3097
                     /locus_tag="ACV5NJ_002394"
     CDS             2330..3097
                     /locus_tag="ACV5NJ_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HHY6486433.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3123..3668
                     /locus_tag="ACV5NJ_002395"
     CDS             3123..3668
                     /locus_tag="ACV5NJ_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486434.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3669..4235
                     /locus_tag="ACV5NJ_002396"
     CDS             3669..4235
                     /locus_tag="ACV5NJ_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486435.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4250..4498
                     /locus_tag="ACV5NJ_002397"
     CDS             4250..4498
                     /locus_tag="ACV5NJ_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486436.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4517..4756
                     /locus_tag="ACV5NJ_002398"
     CDS             4517..4756
                     /locus_tag="ACV5NJ_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486437.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4773..5357
                     /locus_tag="ACV5NJ_002399"
     CDS             4773..5357
                     /locus_tag="ACV5NJ_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486438.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5464..5745
                     /locus_tag="ACV5NJ_002400"
     CDS             5464..5745
                     /locus_tag="ACV5NJ_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HHY6486439.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5735..6076
                     /locus_tag="ACV5NJ_002401"
     CDS             5735..6076
                     /locus_tag="ACV5NJ_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HHY6486440.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2292 a          799 c         1274 g         1760 t
ORIGIN      
        1 tttatggaag aaaaattaat taaaaaggat gagattataa tggggttact ggttccgaga
       61 gctaatactt atactatacc tttaacgaaa cgccaattaa ttgctaaaag atatcaaagg
      121 attactagag ccatcaatag agagttttgg aattctgaaa gtgatactgc tcacagttta
      181 tacgtaggtt cttatgggag aggaacggca attagtacta gtgatataga tataatagtt
      241 gaattaccga tggcagaatt tgacagattc aaaaattatt tatcaaatgg cccgtctaag
      301 ttattacaag ttataaaaaa cgcatttcag gaaatactac caaatagtga tattagagct
      361 gatggacaag ttgttaaaat aaatttccat gatggaatca aatttgaaat agttccagct
      421 tttaatgaaa aagattattg gggtgagtca aaagggttta tttatccaga ttcaaacatg
      481 ggagggaact ggaaagctac aaatcccaaa aaagagcaag aagccatgaa attaaagaat
      541 acaaaaagta ataatttatt atatgctacg tgtaaacatt ttcgacatgt acgagataca
      601 gaatttacga gttatcatct ttcaggaata gtaattgaca gtttcgtata tgaggctatg
      661 ggaaattgga aattcgttga aaataacagt gggggtcaaa acattagttc tgtatcttat
      721 gagacagctt tattagaata ctataactca cataaagtta tgggcggttt aaatttatac
      781 tctcctggaa gcaatcaatt tgttaattct gatagtagta tcatatgttt agaaaaagta
      841 ttaaaaaaaa tagctttata aagttttatg gaggaacaac atgggagacg aaaaaaagcg
      901 cttggaagtt caattacgtg aagcatatgg acgagtaacg tatacttaca ccactcattt
      961 aaagcaagcc gataatatta tttctgacaa taaaatttta aggtgtcttc aaattggttt
     1021 gtcagccctt actactggag gggtacttag taccattatt actgataata gtgagttaac
     1081 ctttgttgct ggattatgtt caacaatttc tcttggatta aacttatact ttaagaattt
     1141 tgatcttgct gaagaagcta aaaatcatca aattgttgcc gataaattat ggttgatcag
     1201 agaagaatac gtaagcttac taacagattt aaatcgttta agtgttgaag atattgtgaa
     1261 aaaaagagat caattaatga acgaaactca tgaagcatat aaaaatgccc ctaaaacaaa
     1321 tagtaaaagc tatgaatcag ctcagaaagc attaaaatct gaagaagagc aattttttac
     1381 agaagaggaa ttggataaga tgttaccaga gcatcttaga aaaaagagtt ctaaatattg
     1441 aactcttttt ttatgcttaa aattctcttg taatttttct attcatctat atttaagaag
     1501 aaatatgatg atagaaacat ctcctaaagc caccgcaagc cgttctaagc atagcttagc
     1561 ccgacattgc tagatatgct catctcacca tgaacgtttc ctaaagccac cgcaagccgt
     1621 cctagccatt gcctagtccg acatcgctag attagtgcat ctcgccataa aagttttcta
     1681 aagccaccgc aagccgttct aagcatagct tagcccgaca ttgctagata tgctcatctc
     1741 accatgaacg tttcctaaag ccaccgcaaa ccgtcctagc catcgcctag tccgattttg
     1801 cttgatcggt tccatttctg tttatttagt gggtcaacac cgtaatctta aatagccatc
     1861 gcctatttaa gattactagg tctgctttga aggtaaaaag tctttcattc ttcaagactt
     1921 ttaggctttc aagctacgcc tgaaagcatt gaaattcatc gctctttatt tggttttgcc
     1981 atttataatt ccttacgccc attatctttt tcgcctgtca taccttgtca gaagtgttta
     2041 gagcttatct catctttgga ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt
     2101 tacgaaaata atttacttaa acacttgtat taatccggca gataagaacg cattctaatc
     2161 actcccgaca aggcaagaca ggcgaaaaag ataaataaat gaacgaaagg aagaaacata
     2221 aaatgcaaaa ccaaatagcg atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa
     2281 aaaagaattt tgaaaaacca aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag
     2341 aattttagaa gttaatcaag aaatggtaaa caagttaaca aatatcagtg ggaactatga
     2401 aaagtacgat ggtactttac ttgataacta tatttttcac gatacacaaa atatcagaat
     2461 tggcagaagt aaaccgagag cgttcattct catcaaagag aaatatttga atgaatggaa
     2521 tagcacctat gaaatggtta tgactgataa ctttgaaaag gtattggatt tcagaaatgc
     2581 tttgttgggt tctgatatgg gaaatagcag tcttgaatct attgaagttg aaaacaagac
     2641 cgagaatgaa ggtgaaggat tgattaagaa tgtcttgaaa atcgaacaaa aatatatcag
     2701 tgaagataga agtttttata gagtcacaag ctcggttggt ctagcaaact ttgctactaa
     2761 cttaattgga aacaatgcta gtgaatcttt gctagtgatg tgtctgaata caaagaatga
     2821 ggtcattgct tatagcgaag ttttcaaagg tggaattaat caatcagtag cgataccaag
     2881 agagattttt caactagctt tgctaaacaa ttcagcaaaa atcgcagtat cacataatca
     2941 tccgagcgga tatgaatttc caagtgaaaa tgacaaaagt tttactacga atttgaaaaa
     3001 cgcaggaaaa acaattggta ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac
     3061 aagctattac tcatttcaag aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca
     3121 agatgggcga aagaacacaa gcgttaatta cagtcaaata tggtaacagc aaaacgaaag
     3181 caagcgtgtc gattcattat cagtgggggt atggtcgata tatgctgatg gacgtattga
     3241 atcaaggaat ttatttaatc aataaagcat acgacttacg agataaatca agagaagaat
     3301 tagcaaaagg aatctttgat agaagtacag gagttatcaa ttttgtcacc tatgaatctg
     3361 gagtgagtgc ttatgatgaa acagggtttg gtgggcgcta tgaaccttat caagaaacga
     3421 ttgaagatgt gatgaagttt tcagataaca atgatggcta tgctcattta gagatggtgt
     3481 tcgatgaaaa tggatttagt ctagatgata aaaccagatt gcgattgttt gattttgagg
     3541 gtcaacagat aggactaaac aagttttgtg aaagcggaaa agagtttgct acgaaagatt
     3601 ttattaaggg atacagatta ttgctagaaa attatggaat aaacctagaa gaagagagcg
     3661 tgaaataagt gtttgaaaaa agaaaagacc agttccctaa tttagaagcg aaaagtcaaa
     3721 aagtgattga tggtgaaatg attgaattat tccaaaaaag ttatggaacc atagaaacta
     3781 ccgcaagttg caaatactac tttcaaggaa agagaaacac gtttaaaggc tttgccgatt
     3841 ctgatgaaga attggcaaag aaaatcattg attggcttgt cagcagtgaa cacgtagcaa
     3901 gagaagatac cgttagaaca acgattaatg gggaagtcct aacaggtact agatgtgttt
     3961 gttgtaatca tgaatttgta gaagatgaag gttgctaccg aatcaatgaa tatggtgatt
     4021 ttatttgcca gagttgccac gagatagaat ttccaaccga aagaagttgg tttgaataca
     4081 tgctacaaga ttatgagatg gaaacagaaa atggtgaagt agttttttgt accaaagagg
     4141 aattgagtaa gagaagcgat gatgaaattg aagattggtg tgtgtggtta gtagggacag
     4201 atgattgcgt tgcatattac acaacagcag aataggagtg tgaatcagca tggaaggaat
     4261 ggattttata gatcatgaag atttgattga ttttggttat acatggaaag gaatggtagg
     4321 tattagcaga agtcttgcta atgcctttta cgaaagaaat tatgcagtct atgtccttta
     4381 tgatgacaat acagaaagcc ttgttgatga agagtataaa ttggatttag agaatgtttt
     4441 atatgggata gaaaaagaag atttagcaaa atatattttt tcatggttgg gtcaataaac
     4501 gaaagagggt agcaacatga caaaattcaa aagtaaaggt atgcaatggt gttcttatta
     4561 ttatgttggg aatgatgaag agcttttgag aaaagccaaa caaaaaggtt atccagataa
     4621 gatgttaaaa cagattggct attacatgcc agaagagaga atagaaaaag aatcttgtaa
     4681 ggttcttttt gatagtgaac agctattagt tttgttaact caaaataaat tgatttgtca
     4741 atttgaaaat aattaatatg gggtgaagaa ccatgaatga gaataagatg gtattaagtg
     4801 aatacgcaag attgagaacc attaattggt tgaatggtat gcaaagtagt ggcgcttttg
     4861 atggatttga tgaggagaat atggactttg aaagtttatc gaatgaggat attagatatt
     4921 attttatcga atggtatttt acagaaccag atgaagaggt ggaagatgaa ccagaattaa
     4981 ttttagaacg tcaaagaatt ttattcagtg aacaagatag taagaggttg tatggtttat
     5041 atgacttaca actgaaagga ttaaaagcgc agtatgttgc tgatagttac gatgatatgg
     5101 tagaacaggg ggcagatagg gttgtcactt attcagaaga tgatgaagag gaatgggaaa
     5161 actattcaga tgatgagata tgctttaatg atgggtatat gcctattgaa atcaccgaag
     5221 aagaatatga taaagtgtcg gatagcgatg agattggtat gtgtacggtt gtaagtggat
     5281 tggatataga tgtccaaaga gtagaggttg cataccagaa gatgaaagaa cgtataaaaa
     5341 gtaaagtaga atcttgattt gattggattg tttgcaaagt agctaattaa gagtataata
     5401 taatcataaa tgtagttaat ggatataata atgagtatat tatactcaaa agaggtgaac
     5461 gaaatgaagg taaaaaatcc aaaagaaaaa agcagaattc aagtcggaat agacaaggat
     5521 ttaaaagaaa acgcagaaat gatattggaa gagctagggt taaatccaac aacagcaatc
     5581 acaatccttt ataagcaagt ggtcgctaga ggtgagtttc cagtagagat aaagctaagc
     5641 gaagaggaaa agcaaggaat cagattgcaa cagctaacta tggatatgcc agttgatgtt
     5701 ctagatacag atgaaaagtt agaagagtgg ttcaatgaag cctaatgaga tagtaacggt
     5761 ctatgttgca tttacagata aaaatggtgg taaaagaaga cctattttgg tggttagtga
     5821 taaagaagat agagtagaat tttttggaat cacaagtcaa tatgagaata aatcagacca
     5881 aataaagaga gtgtattttc ctattgcaga atgggaaaaa gtaggtctaa agaagcaatc
     5941 atggattgac gtagggtctt taaaggctat tcccaaaagc cgagagaaca tatcatttaa
     6001 aaaggttggt atgctatcag ctattgatac gaaaaagcta aataggttta tcgagagatt
     6061 acaaagaaaa gtctaacaag gcttttcttt ttttgtttat atgtatatta agatatacgt
     6121 ttata
//
LOCUS       DBKOIJ010000153         6066 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000153 DBKOIJ010000000
VERSION     DBKOIJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.156"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(254..541)
                     /locus_tag="ACV5NJ_002402"
     CDS             complement(254..541)
                     /locus_tag="ACV5NJ_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HHY6486441.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACV5NJ_002403"
     CDS             complement(561..785)
                     /locus_tag="ACV5NJ_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HHY6486442.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACV5NJ_002404"
     CDS             complement(1240..1410)
                     /locus_tag="ACV5NJ_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486443.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACV5NJ_002405"
     CDS             complement(1425..1958)
                     /locus_tag="ACV5NJ_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HHY6486444.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACV5NJ_002406"
     CDS             complement(1979..2923)
                     /locus_tag="ACV5NJ_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HHY6486445.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACV5NJ_002407"
     CDS             3560..3646
                     /locus_tag="ACV5NJ_002407"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HHY6486446.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACV5NJ_002408"
     CDS             complement(3767..4456)
                     /locus_tag="ACV5NJ_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486447.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACV5NJ_002409"
     CDS             complement(4463..4741)
                     /locus_tag="ACV5NJ_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486448.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACV5NJ_002410"
     CDS             complement(4701..5615)
                     /locus_tag="ACV5NJ_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HHY6486449.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACV5NJ_002411"
     CDS             complement(5597..5977)
                     /locus_tag="ACV5NJ_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HHY6486450.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DBKOIJ010000154         6044 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000154 DBKOIJ010000000
VERSION     DBKOIJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.157"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(118..1569)
                     /locus_tag="ACV5NJ_002412"
     CDS             complement(118..1569)
                     /locus_tag="ACV5NJ_002412"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HHY6486451.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1890..2654)
                     /locus_tag="ACV5NJ_002413"
     CDS             complement(1890..2654)
                     /locus_tag="ACV5NJ_002413"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HHY6486452.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2682..3380)
                     /locus_tag="ACV5NJ_002414"
     CDS             complement(2682..3380)
                     /locus_tag="ACV5NJ_002414"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HHY6486453.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3392..4171)
                     /locus_tag="ACV5NJ_002415"
     CDS             complement(3392..4171)
                     /locus_tag="ACV5NJ_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HHY6486454.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4187..5131)
                     /locus_tag="ACV5NJ_002416"
     CDS             complement(4187..5131)
                     /locus_tag="ACV5NJ_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HHY6486455.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5177..5956)
                     /locus_tag="ACV5NJ_002417"
     CDS             complement(5177..5956)
                     /locus_tag="ACV5NJ_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486456.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1645 a         1264 c         1004 g         2131 t
ORIGIN      
        1 gtatgcccaa aaatttatct gtaatgcgtc atcaaaatct agattccaaa tctttatcaa
       61 tttttatcca ctcattctac cactttagta atattttcta tttataacac tttcgtccta
      121 cattgatccc cccctctgaa gtacagtttt tactgtctta aataaaattt taaaatctaa
      181 ccccatactc caatttttta tatattccgt gtcaagtttt gctacttctt caaaatcagt
      241 aatatcactt ctaccactta cctgccacat tccagtaatt cctggcttaa tagcaattct
      301 tgcacgatga tgataatcat attcttcata ctctgattta aggcatggtc gaaatcctat
      361 aaggctcatc tttcctgtta gaacgttgaa aaattgaggg aattcatcta aagacgtgga
      421 tctaataaat tgaccgattc ctgtcttttt tgttccatct ggaagtattt tatttccgat
      481 aattcttgga tcaaactcca tcttaaacat aagattattg ctcatcttat tttctttcat
      541 taattctact tttctttctt ctgcatccat atacatactt ctaaacttat acatcttgaa
      601 cggtttacca ttctgaccta tacgttcttg tgcaaagaaa attgggcctg gtgaattaat
      661 ataaataagt ggagcaataa agataaaaat aattcccgtt gcaatacatc caacaatacc
      721 tgcaagaata tcaattgttc gttttataaa tgcctgttga gttgtcataa cattgatact
      781 tgttgtaagt actgtatatt ctcctatacg accaatagat tgttttccca aagcactatc
      841 tgatactttt gcaagattca aatgtacagt aaggcccatt tcagagcatc gttcaataaa
      901 ttctttcata cacgaagtac ttttattcaa aataataaag acttcatcta cccatccctg
      961 acataagtaa tccgctgcat ttttggaatc agaaacaact gtcactccct caacatcttc
     1021 accaatcatg cttttatcta tgataatgac accatttatt ttatacattt tatagttatg
     1081 ctctttcaca ttacgaataa ctacttcagc aatactacta gatgtaacta taataagtga
     1141 tttgttcccc tcatttttca ttttatgttt caagtaatgt ttccataaga ggcgagttat
     1201 atatgtaatt acaacgtata aacctcccat caagaaaaga acgactctag aataatcatt
     1261 tccaccctga acactaatga gatacaaact tcctaacagc accaacataa tcgcttgctt
     1321 tacagaagaa gcaaattcta tatagtaccc tctttttaat acacctctat atgcttcaaa
     1381 catgaaaata actaaaagat ctgccagttc aatgaagact gccatgtttt gatacattgg
     1441 aatcacataa ggattccaat ctacctggcg taagacatac gatagataaa aagcaagctg
     1501 gatacacaat aaatctaaaa taataaaatc aatatgttta acccatcccc ttgaactttt
     1561 cttgtacatt cctttttact cccctctttt taataaaata aattgtatta cttgttttct
     1621 ctcctaaata tctttctgaa ggattattga ctcgacaaat tggcttgtgg atattcttat
     1681 atatagaaca tccgtaagcc aacttaaaac tccttaacaa aattttttta ctcatattgt
     1741 agatcaaaac aagtatccaa gaaatcatat tttatttctt ttttctgctt actcaacaac
     1801 agctaaaaga aaaatcatca taaacaaatt caactaagcc aacgatcccc ttgtgaaatc
     1861 aagttttttc ttgatttacg aataaagagc taaaataaac caaacttctt cttttttacc
     1921 tcttcataga ctggataact gatttcatcc ccgttgatca gatcctttgc gacttgtttc
     1981 atctgtacta ctttttcctt accaaagtcc tttgcaatct gctcatagca ctcttttaag
     2041 taaaaggtgc gcttagagac cccatgggca tcagaagcag ccatctgcac gagattatgt
     2101 ttgaccatcg ttttggctgt tttctggatg tctttgccaa ataccccaac ataacttggc
     2161 gcagtgagtt gggccaaaca tcccatatct aggaacggaa gcaaatgatt tgggtcttcg
     2221 cgaaaatgca cgttgcgttc tggatggacg attactggtg tctttcctag ttctaacaac
     2281 tcaaaaaaaa gccgttctgc atataacggc acttccagtg tcggaaattc gatcagaaga
     2341 tatgtatcat ctaaatctgt aaataatatt tcatctcggt ggatgtcttc gatgagcgtt
     2401 ccggtaatac gtacttcttg tccttccaat aacgtcaaag gcaactgtct tttctctaat
     2461 tcggcttgta acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca
     2521 ttattgtggt gtggtgtgca taaaatatgc gtgatgcctt gctgaatcgc tttttcagcc
     2581 attgcaatac ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa
     2641 tgtagatcga tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa
     2701 ccagcatctt tgctatgttc tgctccgttg tacacaacac ctaagactcg tgcttgtacc
     2761 atctccagca gttcttttgc tttagtcaag gattctttac gtgacatatt ttcacgtacg
     2821 accaataaag tgccatccgt ttttaaagac atgatctgtg catctgttac ggctactact
     2881 ggtggcatat cgaatatcac cacgtcaaat aactgtcttg cttcggatag gatctgatcc
     2941 attcttggcg agcttaacaa ttctgatggg ttgggtggct taggtccact aggcaaaatc
     3001 gacaagttct cgactggcgt tcgttggatc acatctgcta cacttcctga tgaactaagt
     3061 gctgtactta atccactggc attgttcagc tggaatgtct tatagacgac gggtttacgt
     3121 aaatctgcat cgactagtag aactcgttga cctgatttag caaaaacgac tgcaatgttt
     3181 gctgcagtgg tggatttccc ttctccagga cctgatgaag tcacgacaat cgttttgatt
     3241 tgctgaccag ctgcggaggc aaactggata ttcgtccgga tcgtacgata ttgttcggcg
     3301 attggtgaag atgggtttgt taatgtgatc aaactgactg catgagtttg tgctgctttt
     3361 tgtttctgtg ttcgtgccat ttttcttcct cctagactct tgagcggctt ctgcgtgatg
     3421 gggctgtttt atcgtttgta gcttttggtc gtgcaggagc tgttcctttt ttacggatac
     3481 cagcgttcaa ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg
     3541 tatctgtcac atatttttca tctttcactg tccgatcgag taattccaat aagaatgcca
     3601 gacctacacc taccatcatt cctaacatta agccagtcat taaattcaat ttattatttg
     3661 gcgaaacagg tgtcgtattg gcaactgcac ctgaaatgat cgagatttta tcaacattca
     3721 tcacatcttt tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag
     3781 ccactgcatt cgtacttatt gcctgaatcg agaacatttg tgaattttct gcctgattaa
     3841 ctgagatggc atctttgatt tgttcagcag tcattttcac attgtcttct gtttctaaac
     3901 gatctttgac ttcattgagc actaaatcac tgacgatcat atctttatac gtattgatca
     3961 tttgcaggtt cgtattgaca tcattgacat ttgcagtgtc tgattgtggc agtgtcacga
     4021 tcaattgtgc ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga
     4081 tggccaagcc agcaaacatt gagatgatga ttattaccaa gtgtttgcgt aatgtttcaa
     4141 aaatttcttt caagctgatt gtctcttcca ttttttctcc actttcctat ttattctcta
     4201 gattcatttg atcttttaat tcttttttca cacgatcaag ttcttgatca ctgacttttt
     4261 gataggagat attatcttgc atgaagcctt ctccttgcag ctgatcttgc ttaatcgtac
     4321 caaatgcctc ccgataatct aaagcaattt ttttcatttc atcaaagttc atgtctgttt
     4381 tcatattcga ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttagactca
     4441 atactttttt cgctacgcct tctacgatct ttctttggcg cccttgtcta ccgtaatcac
     4501 catttgggtc ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaatgtaa
     4561 ttttaccgat tggaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga
     4621 taccaccaac tgcatcaacg agttccttta gtcctttcat attgatagag acatagtgat
     4681 cgattgggat gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg
     4741 cgtaagcgtg gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatctc
     4801 gaggaatgct gacgatcgtt gtctgtttgt cttcaggact gactgtggca accatcatcg
     4861 tatctgatct tccttgatcg acccgaccta aatctccagt atcaatcccc atcaatagaa
     4921 cagagaaaga ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt
     4981 ccactgattc gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta
     5041 ctcctacaat tgctaatact aatacgataa gaactgctaa aataatcaaa aatatttttt
     5101 tccctcttga ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga
     5161 atcaacgttt taactgtcaa tagcttatat tgctatagcc catttgctga gcaacagttt
     5221 gtgcttttgt tgcgtatgcg gctcttattt tttcattccg gatctgctct atttgactga
     5281 gcgccgtcaa atattgatcg taagtagcgg tagctgttac ttgaccgtct ttcagcatcg
     5341 tatcgaatag ctgatctagt tgcgtgactt gtgcaatttg ggtatcagcg aatcctagat
     5401 attgcataac gactgttttc caagcactat ccttaaaaaa gctcatattt tcacgtacat
     5461 gggcgacatc tgcacttgcc aacttttctt tgatgatgac atcatccaca gcttgctgcc
     5521 agtttgatac gtttttagta aataatttat tgacagccgt ctggatcttc agttttgatt
     5581 ctgcatcctc taactgcgtt aagactgctt tcttttcttc ttggatcgca gcagcttctt
     5641 taggtaattc tgattccttg atgtcataat catctgccga aagtttgata gaagatagtt
     5701 tatgtgacaa atttgaaact gtttcttctg ttatttcagg atttaggaaa attttttctt
     5761 ttgtactgta cagcttatct acttcttttg ataaagaggt cacggtgtct ttttgattgg
     5821 tgatggcaga ttctgcgtaa ttgatccgtt gatgatattg ccgctgatta ttgagatggt
     5881 atccaaacaa gcaaatcaca atagcgcctg tgcctaccgc cacagtagcc aataacttga
     5941 cgaacggctt ttccatagat tcactcctaa atattgatga tagataagta aagatgtgat
     6001 tcattcacat cagaatgagc gctttttatt ttaaaagcat aatt
//
LOCUS       DBKOIJ010000155         5787 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000155 DBKOIJ010000000
VERSION     DBKOIJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.159"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            65..961
                     /locus_tag="ACV5NJ_002418"
     CDS             65..961
                     /locus_tag="ACV5NJ_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HHY6486457.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1111..2052)
                     /locus_tag="ACV5NJ_002419"
     CDS             complement(1111..2052)
                     /locus_tag="ACV5NJ_002419"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HHY6486458.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2178..3299
                     /locus_tag="ACV5NJ_002420"
     CDS             2178..3299
                     /locus_tag="ACV5NJ_002420"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HHY6486459.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3557..5059
                     /gene="eat(A)"
                     /locus_tag="ACV5NJ_002421"
     CDS             3557..5059
                     /gene="eat(A)"
                     /locus_tag="ACV5NJ_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HHY6486460.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5126..5653
                     /locus_tag="ACV5NJ_002422"
     CDS             5126..5653
                     /locus_tag="ACV5NJ_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HHY6486461.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1895 a         1023 c         1218 g         1651 t
ORIGIN      
        1 tggcttttta ctatttaaac aggctatcat aggaaaaagt gacagagaaa gtgaggcgga
       61 aaagatgaac aaaaatgacg tactgctagt tgcaaaaaat aaatcacttt attttttgcc
      121 ttccatccgc tctttagatg tttggtccgg aaaagattta ctacagtcgg aattcattaa
      181 atcagctaga caacagttgg caaattatca gctgatcgtt tttttagatt atggatttga
      241 tatggagatg gctcgacgcg tgcgcccatt taccaatgca aaaattgtgt tatttttttg
      301 gaatcacttc aaagcagaac acgaggcgat gctgaagcag gcacaagatg aaccggcaat
      361 cgatgagatt tatcattttg attttttgga agcaaaagaa cttgggctga aacataatag
      421 ttcattttat tcgaaacata tgaatttttc ttctagcgaa ccaaagacgg atctattttt
      481 tggtgcaacg aataatggga gaaaagaacg agcagaaagc tataagcagg aatttataaa
      541 aagagatgtt acgaccaact atttcattct tccacatcga gggaatgaac aacctggata
      601 tcttacttat gaagagtacc tgaaaaaaac aggggaatcg cgcgggattc tggaactgtt
      661 gcgggagggt cagcaaggag tgactttgcg gacatttgaa tccatgtatt ttcaaaagaa
      721 actagtgacg gataatcaag cgattgctca ttatcatttt tatcgtcctg aaaatatttt
      781 tttgcttcga gaacgtgatt tagatgaatt accggaattt ctgcatacac catttaagcc
      841 aatcctatca gagctattgg aattttatga tgcagaaaat tgggcaaaac gatttatcac
      901 taacgattcc actatttatg agcaatacga atatgacctc tcctctgttg aaaacaaata
      961 aaaaacctac attttgaaga gattatgtac tgaaacaaac aagcacaatt cttatggcat
     1021 catgagagtt gtgttttctt tttgatcttt tattgggctt aaaaaaagag aaccagctgc
     1081 tgattcagct gattctcatc agagagcctt ttattctgcg ccaaggattc cttcttttgc
     1141 atggactaat tgggtcaaaa aggcacaata tggtgtagca atatcatatt tttgtccttt
     1201 tcgccaaatg gcaccattga tgtagtcgat ttcagtcaaa cgatggtttt tgatcaggtc
     1261 ttggtacata gatgggtaat gcaacccgat gttgtcagga tcataagtag aagcaatatg
     1321 ttggtacact tcttcttgat ctagtatgat tccttctttt gctgcgatag aagcaaattc
     1381 agatacgatt gttcgaacca tgctctctgc cgctttttgc gcgcctagtt cagcaatatt
     1441 acaatccaaa atcgtgcata aaccattcaa tgttccattt acacaagctt ttcgccaaat
     1501 ggaataacga acgttatggc tatagatcgg atttaaattt gcttcttgaa agacttctac
     1561 aacttttttt gtaaatgctt cgccttctgg ttccaagttc tctagttcga tttctccatc
     1621 acctaacaat gtgacttttc cgggaccagc tagaccagct gtccacatcg tgatcccaaa
     1681 taagatattt ttctttggca catatttttc cagtacatct tcatgaccta atccatttag
     1741 taaacacaaa acataagtat tgtcagtaat aatggattgg atcgaacaaa acatatcatc
     1801 taattgattg gcttttgtta atgctacgat caagtccacg tgttcgttgc tttcgatgat
     1861 ttcttctggt gaatagattg gcagttttgc gaccacttct ttaccgttaa agtcagcaat
     1921 caacccattt gtacgaatgg cttcaatgtg agcaggccaa cgatcgatca agaggacctc
     1981 atttccgctt tgatgtagca tcaatccgat tcggctgccc attgctccgg ccccagctat
     2041 tgcgattttc atgttttttc tctcctttat tcaatgagtt cccttttagt atatagaaaa
     2101 atggaagaaa atagcattgt tttgtttgaa aaaaatgaaa aaaagtgtct tacttaagaa
     2161 gaaagtgagg gatacagatg gatattttgg cggcaattga ttcatttaaa ggaagtgctt
     2221 cttcatttga actaaatcag gcagttctaa atggctggga agatcgtaaa ggaagaaaaa
     2281 tcaatgtgcc gattgcagat ggcggagaaa atactgcaga agcaatctat tacgcgctca
     2341 ggggaacatg gcgtaccatt gatgggatgg atctcttatt ccgtcagcga aaaatcagct
     2401 atttattgac aacttggcaa ggaaaaaaca ccgcagtcat cgaatctgca caagcattgg
     2461 ggttagatct acttgataaa ccaacggatc aaactatccg acttgcttcc agttatgcct
     2521 taggagaact tattaaagat gcactgcttc aagaagtaga acaaattatt gtcactttag
     2581 gaggaagcgg gtgtagcgat ggtggtctag gattactcca atccttgggt gcttctttag
     2641 aagggtacac agaaggaaat cctttattat ctactaaaaa acttcatttt gatcgaagaa
     2701 acgttcttgt ttctaaagta aaattgatta tcgctgcaga tgttaccaat ccatatacag
     2761 gtcaacaagg agctgcttgg caatttggaa gacaaaaagg aggaatagat acgacgctag
     2821 ctttcttaga taaacaagca ggaaagattg ctcagcaaat aaaagatttc tgggaggtgg
     2881 atttagatga tattccagga agtggagcag caggcggtat tggtggagcc ctttttttgt
     2941 tgggagcgaa gatggtttct ggtttcgatt tagtcagcca aatcactgga ttggaaaaaa
     3001 agatcgaaca agcagatgtc atcattacag gagaagggcg gatcgatcag caaacgatcc
     3061 acggaaaagt tccatacggt gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg
     3121 ctatttgcgg aagtagagac caagagctag aaggactttc tgattttctc ccaattgtct
     3181 taagtattca tttgggtcca ataggtttag aagaagcaat ggaacacagg caaacattag
     3241 aaaaaacaaa aatcttagga cagacactta gcagattttt caccatcctc ccgaagtagt
     3301 tgacaaaatc attttcttta tgctatcgta atttcaaata taggacaaag tgagggatcc
     3361 gtgcattgaa aaattaagtc gattgatgaa agctgcattc agactgtacc ttgtggagac
     3421 aaggaagcag tgtgcagatt ttcgggattg gcttggtttt ttcgatgtat gggtcttttt
     3481 gtgtgtatcc aacccttgca tcctctttag ccagttctga aaataggact ggtctttttt
     3541 tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat ctgacattcg gctacgacag
     3601 ccaaggcaca ttattatttg aacaagcaaa tctaaatttt gacacacaat ggaaactagg
     3661 acttatcgga cgaaacggtc gaggaaagac aactttactg aatattctac aaaacaaact
     3721 accttatcaa gggcaagtaa tccatcagca agaatttgcc tatttcccgc aacagacaaa
     3781 agataaagaa cgtttaacct attacgtgtt aaatgatatt acggattttg agatatggga
     3841 aatcgaaaga gagctccaat tgatgcaaac agatcctgaa atcttatgga gagaattcag
     3901 cacactatcg gggggagaga agacaaaagt cctactggca cttttatttg tggatgacac
     3961 tcatttcccg ttaatcgatg aaccaacgaa tcatttggat atctctggta gaaaacaagt
     4021 agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg gtcagccatg accggggatt
     4081 tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa agtcaactgg aactttatca
     4141 agggaatttc tctatctatg aagaacagaa aaaacttcgt gatgaatttg aaatggctca
     4201 aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa acagcagctg aaaaagccga
     4261 atggtctcgt tcccgagaag gagataaaac aaagaaacaa gtcggattca tcgatactga
     4321 atctagacga gtgaataaag gagcagtggg tgctgatgct gcacggacga tgaaacgatc
     4381 caaagcaatc gtgaatcgga tggagaccca gatcagcgag aaagaaaaac tattaaaaga
     4441 tatcgaatat atcgattctt tgacgatgaa tagccaagcg tctcaccata agcgactttt
     4501 aagcgtagaa gatcttcaat tagggtatga aaatctgtta ttcgagccaa ttcattttac
     4561 aatcgagcct catcagcggg tggcgatttc aggtcctaac ggtgcaggaa agtcatccat
     4621 tatccattat cttctggggg cattcaacgg caaggttata ggagaaaaat accagccaaa
     4681 acatctgagc attagttatg caagccaaaa ttatgaagac aatcgaggaa cgttggcgga
     4741 atttgcagag aaaaaccaag tagactacca agcatttttg aacaacctcc gaaagcttgg
     4801 gatggaaaga gatgtttttc ataacaagat cgagcagatg agtatgggcc aacggaaaaa
     4861 agtggaattg gctaaatctt tatcacagcc agctgaacta tatacatggg atgaaccatt
     4921 gaattatttg gatgtcttca atcaagaaca attagaacaa ctgatcttga acgtgaaacc
     4981 tgccatgtta ctagtggaac atgatcaaac cttcctggat aaagtatcta ctgagattat
     5041 ttctcttgag agaatctaac ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa
     5101 ctttgccaac gaaggagaga atcacatgct ttatgtaacg agacacggag aaacaacttg
     5161 gaacgcacaa ggattagttt gcggacgggc agatgtgccg ctgactgaaa aaggacagat
     5221 gcaggcacag aaactagcag aaaaggtagt agatttgcct gttccaatca cgaaaatcat
     5281 ccattcccct cttcaacgag caagagatac agcacaagca gttgctgatc gtctcagctt
     5341 gccgctgacc gttgatgaac gattggtaga aatggatttt ggggattatg atggactgcc
     5401 aagcaaagat gagaactttc aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg
     5461 ggagtcagtc ttagatgtgt atgcacggat cgttccttta ttgaaagaat gcatcgaaga
     5521 cgaagaaaat gtctatctgt tagtttgcca taatgccttg ataagagtga tcaatgctta
     5581 ttttcatccg atgcctaatg aggggttctt tacattcatg gtagataata cagaattggt
     5641 ttcttatgag taattatgtt tttgtgttat ttaaataaaa tatttcaagg cactgctttt
     5701 gcttagcagt gcctttttta ggttatgaca attcttttga taatataaac tcaaccttta
     5761 gaaatgaaat ttaattattt taataaa
//
LOCUS       DBKOIJ010000156         5734 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000156 DBKOIJ010000000
VERSION     DBKOIJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.160"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            119..490
                     /locus_tag="ACV5NJ_002423"
     CDS             119..490
                     /locus_tag="ACV5NJ_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486462.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            575..775
                     /locus_tag="ACV5NJ_002424"
     CDS             575..775
                     /locus_tag="ACV5NJ_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486463.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            909..2135
                     /locus_tag="ACV5NJ_002425"
     CDS             909..2135
                     /locus_tag="ACV5NJ_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486464.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2732..3127
                     /locus_tag="ACV5NJ_002426"
                     /pseudo
     CDS             2732..3127
                     /locus_tag="ACV5NJ_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            3205..4244
                     /locus_tag="ACV5NJ_002427"
     CDS             join(3205..3702,3705..4244)
                     /locus_tag="ACV5NJ_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HHY6486465.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            4275..5195
                     /locus_tag="ACV5NJ_002428"
                     /pseudo
     CDS             4275..5195
                     /locus_tag="ACV5NJ_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            5188..5535
                     /locus_tag="ACV5NJ_002429"
     CDS             5188..5535
                     /locus_tag="ACV5NJ_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486466.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            5532..5714
                     /locus_tag="ACV5NJ_002430"
     CDS             5532..5714
                     /locus_tag="ACV5NJ_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486467.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT         2129 a          828 c         1132 g         1645 t
ORIGIN      
        1 atttggataa tcatgtgaat atgaacttaa caattaaacg tatttacgat cttttatcac
       61 ttatataaag agagaagtga taaataggac acaaaatttg gaaagtaggt aagtcactat
      121 gagagaacta actttatctt ctaaggaatt agaaaagaaa actgctttat taaacgaaat
      181 atttagtgct gattgtataa tccaaagttt aaaagaaagt tcattttcaa aactaacaga
      241 tcaggaaaaa atttcttata tgctaagttt agataatcta ggaactaaat atgatgaatc
      301 agcaagtagc gataagcttt tgattgaatt agtatcaagt cttagtagtt ttggaaaaat
      361 gaaaagattt aatgtgatat tagaaatttt tgcttggtta atcccttcat ttatttggtt
      421 tttttatatt ttcatagttg aatctgagtt aatagccaag gtctctcttg gactgttaat
      481 tccgatataa ataggttcag caatttactt taataacttg tatacaccta ttcaaataaa
      541 aaagcagtta ttacatgaag gattaagtgg taatatgtta aaaagaattg catgggataa
      601 agataataaa acagatattg agcttaaaga atataaatac aattatttct cgagatgtat
      661 agatagaaag ttactgttta aaaaagaagg attagaattt agttttgatg aagatgaatt
      721 tgaaaaatta gtgatggact ggcgtaagaa tttactggat acggtacaat tctaaaagca
      781 attgaggtcg aaattccgat acttgaatca tgatctatat tatcgtgtta tgagtgaaat
      841 ttctaataaa gataagatat ttcatttgga cagtcatgtg aatatgagct taacaattaa
      901 acgtatttat gatcttttat atgtttataa acattaatga ggtgctaaaa ttgacaacaa
      961 taactatgac aaaaaaaata gaagtatatg ttgatggatc acaagaagaa agggattcct
     1021 tttttgattt tttttggaaa aatagtagag aatatgtaaa agcgttgaat ctggtatata
     1081 aacagctaat gtttgaagag ttgataaaag atgcaattaa agaaatggat caaggttatg
     1141 ctgagagaga agaaagtatt caaaatcgga ttgaaaagtt agcatatact gatccaatag
     1201 atcaaaaaaa acttgataaa gcaaaagctg aattatctaa attgagaaat gaaaaaaata
     1261 aagaagcgag agaaatatta acgcaagcga tagggttgaa gagtcaaacg agaacacgag
     1321 atgtagcaaa aaaattagac tttgactttg gagattttgt tgattccgca aatatgaaag
     1381 ctagtaaaga ttttggcaat gattttatcg gaattgtgac cggacaaaga acgcctagaa
     1441 tatataaaaa tagaaacaaa tatggattat taccttttag aggtcgagat attaaaatcc
     1501 ataaagatga taagttttat atttatttac ctaaaggctt taggtttaaa gtgaatttag
     1561 gtagcaagcc aagaaaagca agagatgttg cttcagtttt acaacacatt atggatggcg
     1621 attacaaatt aaatcaaagt aatcttattt ttgatggaag aaaattattt tttaatctaa
     1681 caatttcttt tgaaaaaagc tataatgaaa tggaattcaa gaaaggtaat gaattgatcg
     1741 taaactttgc agatttgtgt agcggtgaat gtttaattaa tgataaatct gtaatgtctt
     1801 ttggtgatag ccaatatata agaaacttca aaagtaaaat tttagcaatg aaatcaaaag
     1861 aacaaagccg aggagtcttt tctaagggcg gtcatggtcg aaaaagaaaa ttagatcaag
     1921 atcgatggga agcaataaag aaaagagaaa gtaattttgt taaaacttat aataatcaat
     1981 gttctgcaaa aatcgttaaa actgctattc gcaataaagc tcaaattatt cgtatcattg
     2041 aaccagataa ggcttatggc gaatgggctt actatcaatt gaaagaacaa ctagtaaaca
     2101 aaggaaaaag atatgggata gaagtcagaa gttagtaaat taaacaatcg ttgatttggc
     2161 aacgaaatag tgagggaaaa tccactcact gaagggtaaa ctccgtcaag ttgtgtgacc
     2221 agacggaagg acgaatgttg ttgaggtttt agaaccatat aaaacagcat gtttctcaaa
     2281 tttttaggat aagggaaatt gctagcagtt tgttttagaa ccatataaaa cagcatgttt
     2341 ctcaaatgag tactttaaat taattgatgg cgttcctgct ttagaaccat ataaaacaac
     2401 atggatttaa aacaagaagt aagtcagttc tccttttact ttagtaagga aaaataaagt
     2461 attctcaata aaacttaaaa taagcaaacc tttgttttaa aatgattgtc aagttaaaaa
     2521 aatctgggat catataaaat agtatagttc tcaaatatga ttagaaaccc tattaaagag
     2581 caatattaag ccaacttact taaatcgttt aattaagaac aataatctct tttagtcaga
     2641 cttttagtct gacttttttg tatttccaaa atttattctt tacaaaaacg aacatacgtt
     2701 tgtataatta cttgtgaatg ggggaataaa aatgtttgat tacagtagag aaccggtgaa
     2761 tgactatttt ttaatcgaca tgaagtcttt ctatgctagc gtggaatgta ttgaacgaaa
     2821 tttagaccca atgactacac cgttaattgt catgagtaga tcggacaaca ctggttcagg
     2881 tctgactcta gcttcttcac ctactgcaaa gaaaaaatat catattacta atgttagtag
     2941 agctactgat ttaccttatc cattaccaaa agaacttcat attgttccac cacgaatgaa
     3001 cttatatata aaaaaaaata tggaagttaa tcagattttt agaaagtatg tcgcagatga
     3061 agatttattg atttattcga ttgatgaatc aattttaaaa gtgacaaaat ccttaaattt
     3121 atttactata taaattgagt taaattgtat tcgtttttcc tcctataggc gtatactaaa
     3181 gaaaacactt gaggagtggg gaacatggaa acagaacgcc aaatccgtga attggaaaaa
     3241 gcgatgaata cgacagacaa ccgccggttg tacgagcgtt tcctggcagt caagttggtt
     3301 cttgaaggcc acacccggaa agaagccggg cacaccatcg gccgcgacga gcatactgtc
     3361 agccactatg tgaaaaacta ctgccataat ggaatccaag gtttagtctc caaaaaacaa
     3421 agcggacgac cttcacggct gacagatgaa caggaagcgg aactggtcca aatcgtcgcc
     3481 taccacactc cggaggaagt aggtttcaaa tcccgtgcca attggacatt ggcgattgtg
     3541 tgtgacctga ttctccggga atggagttat ggatatacgc aaaaaggcgt ggcggacctc
     3601 cttcaccggc taggactaag ctggacccgt ccgacgtata ccctcaaaaa agcggattcc
     3661 aagaaacaac aggtattttt ggaagaaacc ttcccgagcc taaaaaaaaa ttgctgaata
     3721 gcgatattga ccacctccta ttcgaggacg aaaccatgat ccgggattac caagccattg
     3781 gctgcacctg gtttctccgc ggccaccagc gaaaaatccc cacttatgga caacacaaag
     3841 gggtaaaatt gataggcacc ttggattatg aaaccggtcg gatcatttgt acagaagaag
     3901 gggcatacga tgcagcggcc ttcctccgtt ttctggagcg gacgttgcgg gaatacccca
     3961 ccggaaaaat cgtgatggta ttggataatg cccttatcca ccacgccaag ctgatccagt
     4021 cttttcttga agaaaacgct aggcgcctgg cactcatctt cctccctccc tacagccctc
     4081 agttgaacct aatggaaggc gtttggaagt ggctcaagga atcggtcatc aacaatgttt
     4141 tttttgacca cgtccagaaa atcaaacagt cggtccgcgg tttcttagcc gatgtaaacg
     4201 agcgtcccct tgaggtgatt gaccgactgt gcgtgcggat gtaaagtatt taattcaacc
     4261 tatatagttc aggaacaaga agcgagagaa gaagagaatt agctaaaata atcaaagatg
     4321 agattcagaa agaaacaggt ctaatagcaa cagtcggaat aggagataac ccattattag
     4381 ctaaacttgc actggacaat gaagcgaaga gtagtaaaga atttatagct gagtggactt
     4441 atgagactgt acctgaaaaa atatggaaaa taaacgagat gactgacttt tggggcattg
     4501 gatcacgcat gaaaaaaaga ttgaatcata tgggaattat gagtatagaa gatttagcaa
     4561 attggaatcc ctacacaatt aaaagtcagt taggtgtaat cggactccag ttatatttcc
     4621 ataccaatgg aattgatcgt acggatattg ctattccacc agaaccaacc aaagaaaagt
     4681 cttatgggaa ttcacaagtt ctaccaaaag attatacgag gagagaagaa gtagaactag
     4741 tagtcaagga gatggcagag caagtagcca ttcgaattag acaacacaat tgcaaaactg
     4801 gttgtgtacg cttagcaatt ggtacgtcaa ttttggaaga aaaatcaggt ttctcgcacc
     4861 agatgaaaat accaattaca gataatacga aagaattaca aaattattgt ctttttttat
     4921 tcaataagtt ttatgagggg caggaagtac gacatatagg agtaacttat agcagattag
     4981 tatacacaga ttccctacaa ttagatttat ttagtgatcc aagaaaaaag attgctgaag
     5041 aaaatttgga taggattatt gataagatta gacaaaaata tggatttaca gctattatac
     5101 atgcttcatc tatgttagaa ggtgcaagaa gtattacgag aagcacttta ataggaggac
     5161 acgcaggagg attaggaggt ttgcataatg attgaatata acgatagagg acgtttaaaa
     5221 tggtcgggat tttatttgtc tgatcatact gaaagaatca atgcagacaa tgtacaacgt
     5281 gctaatcata atttggcaaa agaacaaatg acgactgaag agattgcgaa aatactgaga
     5341 actgctgtca taaaaaatag acccgtctgc attcaaaaag aggaacgaaa cgctgaaggt
     5401 tactatccag atgatgtagt aggaaaaatt attggatatg acgagcttgg tgtatttgtg
     5461 gaagaagaaa aagttcacta tgatgaaatt aggaatgttt ctttctcgga attattaaaa
     5521 tggagtcaga tatgaacatt attaaaaaat acgaattcgg ttatattacg tatgaacagc
     5581 taatagagga attatgggga tatggtcaac aattaattaa tgaagtgggg attgattgct
     5641 tttgtttcta tattgaatcc ggagcaggat accataagaa cagatactat atatttccat
     5701 atccaccaaa atagtctctt agatattttt aaat
//
LOCUS       DBKOIJ010000157         5689 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000157 DBKOIJ010000000
VERSION     DBKOIJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.161"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(488..1297)
                     /locus_tag="ACV5NJ_002431"
     CDS             complement(488..1297)
                     /locus_tag="ACV5NJ_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHY6486468.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACV5NJ_002432"
     CDS             complement(1297..2043)
                     /locus_tag="ACV5NJ_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HHY6486469.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACV5NJ_002433"
     CDS             complement(2061..2528)
                     /locus_tag="ACV5NJ_002433"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6486470.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACV5NJ_002434"
     CDS             complement(2528..2938)
                     /locus_tag="ACV5NJ_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6486471.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACV5NJ_002435"
     CDS             complement(2949..3962)
                     /locus_tag="ACV5NJ_002435"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HHY6486472.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACV5NJ_002436"
     CDS             4179..4913
                     /locus_tag="ACV5NJ_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6486473.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACV5NJ_002437"
     CDS             4897..5607
                     /locus_tag="ACV5NJ_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6486474.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DBKOIJ010000158         5603 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000158 DBKOIJ010000000
VERSION     DBKOIJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.162"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            65..766
                     /locus_tag="ACV5NJ_002438"
     CDS             65..766
                     /locus_tag="ACV5NJ_002438"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HHY6486475.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            763..1884
                     /locus_tag="ACV5NJ_002439"
     CDS             763..1884
                     /locus_tag="ACV5NJ_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HHY6486476.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1899..3131
                     /gene="pepT"
                     /locus_tag="ACV5NJ_002440"
     CDS             1899..3131
                     /gene="pepT"
                     /locus_tag="ACV5NJ_002440"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HHY6486477.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3263..4171
                     /locus_tag="ACV5NJ_002441"
     CDS             3263..4171
                     /locus_tag="ACV5NJ_002441"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HHY6486478.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4206..4478
                     /locus_tag="ACV5NJ_002442"
     CDS             4206..4478
                     /locus_tag="ACV5NJ_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HHY6486479.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4489..5535
                     /locus_tag="ACV5NJ_002443"
     CDS             4489..5535
                     /locus_tag="ACV5NJ_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HHY6486480.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1821 a          972 c         1216 g         1594 t
ORIGIN      
        1 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
       61 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
      121 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
      181 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
      241 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
      301 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
      361 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
      421 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
      481 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
      541 tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga tgttcgggcc
      601 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
      661 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
      721 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
      781 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
      841 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
      901 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
      961 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     1021 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     1081 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     1141 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     1201 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     1261 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     1321 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     1381 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     1441 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     1501 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     1561 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     1621 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     1681 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     1741 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     1801 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     1861 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     1921 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     1981 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     2041 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     2101 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     2161 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     2221 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     2281 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     2341 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     2401 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     2461 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     2521 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     2581 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     2641 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     2701 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     2761 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     2821 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     2881 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     2941 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     3001 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     3061 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     3121 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
     3181 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
     3241 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
     3301 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
     3361 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
     3421 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
     3481 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
     3541 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
     3601 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
     3661 cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga tcagtcaaga
     3721 agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt tattgctgaa
     3781 gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag gaaaatccca
     3841 actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg acgactcagc
     3901 gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata ttttccgatt
     3961 tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg gcattactta
     4021 tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga
     4081 tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag gattaacagt
     4141 ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa gttggaagaa
     4201 gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt gtgaattttt
     4261 acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc tggcgtgttc
     4321 ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga ttgatctctc
     4381 gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc gctgcaggtg
     4441 tacttgttga tttgattctc atttattttt tccattaagg agcaggagat gtcgtctaaa
     4501 ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt tgccattatt
     4561 tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc taccagtgtt
     4621 gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga agggcgctca
     4681 atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg gttcttgacc
     4741 tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct gatctcggag
     4801 atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt gacagcactc
     4861 catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt ggtcatccaa
     4921 ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc tggtatcctt
     4981 ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa tgttcaaata
     5041 ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa atgggtggat
     5101 acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat ccaagacagg
     5161 actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg gtatgtactt
     5221 ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac catttcgttt
     5281 atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat tggttccttt
     5341 gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc agtttcttcg
     5401 cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt tgtgtatgtg
     5461 attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa agagaataaa
     5521 gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa agaaaaacgt
     5581 tatggataag ataattttac gtt
//
LOCUS       DBKOIJ010000159         5599 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000159 DBKOIJ010000000
VERSION     DBKOIJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.163"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            28..1446
                     /locus_tag="ACV5NJ_002444"
     CDS             28..1446
                     /locus_tag="ACV5NJ_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HHY6486481.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1517..2986
                     /locus_tag="ACV5NJ_002445"
     CDS             1517..2986
                     /locus_tag="ACV5NJ_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HHY6486482.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            2996..3463
                     /locus_tag="ACV5NJ_002446"
     CDS             2996..3463
                     /locus_tag="ACV5NJ_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6486483.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3499..4371
                     /gene="scrK"
                     /locus_tag="ACV5NJ_002447"
     CDS             3499..4371
                     /gene="scrK"
                     /locus_tag="ACV5NJ_002447"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HHY6486484.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            4453..>5599
                     /locus_tag="ACV5NJ_002448"
     CDS             4453..>5599
                     /locus_tag="ACV5NJ_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HHY6486485.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAG"
BASE COUNT         1887 a          796 c         1135 g         1781 t
ORIGIN      
        1 taaaaacctt ttcactggag gaagaaaatg gagaaattat taattaaaag caataatcat
       61 caactttact accaacctaa ggatgtttgg gtcggagatg ttatgcctta cgggaaagaa
      121 ggaaaattct ttatttatca tcaacgtgat actagaaatc cagttccttt tggagaaccg
      181 tttggctggg ctttagcaac tacgaaagat tttgttcatt ttgaagactt tggggaatct
      241 cttttaagag gggatgacaa tgagagagac cagtttatct acgctggttc tgtatttgag
      301 gcaaatggaa aggttcacgc attttataca gggtataatc gtgaattctt agcgcaggga
      361 aagacatctc aagtgttgct tcatgctgag agtgatgatt ttgttcattg ggaaaaatca
      421 gaaacagcat tggagttaga accacaagaa gggtatgata ttcgaaactg gagagatcca
      481 tttgtcgttt gggatgatga aaaagaagaa tatttgctaa ttttaggggc gcgaaagggt
      541 caggataaac atctacaaac cggacgttta gtggaattta catcaaaaga tttaactaat
      601 tgggaattca aaggtgattt ttgggctccg ggattgtata ccatgtttga aatgccagaa
      661 cttttcaaga ttggtgactg gtggtatctt gtctattcag aatactcaga tggaaataaa
      721 atactctatc gaatgagtca aagtttggaa ggtccttgga ttaagcctaa agatgaagct
      781 tttgatggac gggcttatta tgcaggtaga acagcttatg atggaaaacg tcgagtccta
      841 tttggatggg taccaacaaa agatcaagaa aatgataaaa acaattattt atggggaggt
      901 actttggttc ctcatgaagt ctatcaaaaa gaggattttt cacttggggt aaaacccata
      961 gacgaagtgt ggaatgcatt cataaattta catgatattg ccgatgtaga gatagaaaca
     1021 gcagacacaa gagaagaagt aacgcttgtc aaaaatagtg gaactttgtt taatttagaa
     1081 acaacaattt catttgatga gaatcttagt gacttttcac tgagattata taaaaacctt
     1141 gagacagatg aatcttacga atatcgcttc aatttagatg aggggcgtct tacatttgat
     1201 aaaaaccctt gttacccttg gtttcaatat atggataaag gacttattag acctatatct
     1261 ttaaagccta accaatctta taaactaaaa gtgattgttg atgacaatat aatgacactt
     1321 tatattgatg gaatcgctct taacgctcgt gtacatggtt gttttggtca agcaattact
     1381 attacagcaa caaatggatc aatgactgca ataaatacca agttttcaaa tgaaataaag
     1441 aaataataaa cgtcaatcat gtggcaggtc tgaaaatcaa acatgacctg tcacatttaa
     1501 aagggaggat aaatgaatgg actacaaaaa aattgccgaa gatgtattgg aaaacgttgg
     1561 aggaaaagag aatatctcaa gcgctacaca ctgcattact cgtttacgtt taattcttaa
     1621 agacaaaagc aaagcgaata tggaaatgct tgaggaaata gaaggtgcaa aaggtgttct
     1681 tttcaatagt ggacagttac aagtaatttt cggaacaggt ataattgaaa aggtttacaa
     1741 agagttcgtt gaattgacag gagctaagga agtatccgtt tctgaaatga aagacgaagg
     1801 agtcaataat tcaggaaata gacttcaacg agcttttaaa gttttctcgg atatctttat
     1861 tccgatcatt ccagctttta ttggggcagc aatggtttta ggaattcgtt cgttgattac
     1921 cactcctgga atgtttggaa tggatggctc attcgctgat cacaatgtat tcctaaatga
     1981 tttatccaaa tttctgggta ttattgcaac cacatttgat tatttaccag ttttagttat
     2041 gtattctgca acaaaacgtt ttggcggaaa ccctatttta ggattacttg taggatttgt
     2101 tatgattcat ccaggactag ccaatcggaa tgatttcgtc atgggaacta taaagcctga
     2161 ttattggcat ttatttggat taagcattcc agcagttgcg tttcaagggg gtgtttttcc
     2221 agcaattttg acttcttggt ttttagcaaa agttgagaaa attacaaata aatattctcc
     2281 tcaagtactt gcttacattt tagtcccaac tgtgacaatt gttcttgcca actttgcact
     2341 atttttagtg tttggtccat tggggaattt tatcggtaat gttttaggtg gaatcattga
     2401 tttcctatat atgaagatgg gtgcctttgg tgcttttgtt tttgcaggcg cgttacaacc
     2461 attagttgta acaggaacac aacatgctat tcaaggtatt gaggcaaact tagtcgcaac
     2521 aactggattc aattatatcc aacctatttg gtcagtttca attattgccc aaggtggcgg
     2581 ttgtattggt atgtatttac tctttaagaa aaaatctaaa gatagagata ttgcgatttc
     2641 aagctttatt ccaacactgg taggtattac tgaaccggct atctttgctg ttaatttgaa
     2701 atattcaatc gttccttttg tttgctcgtt tattggtgct ggttttggtg gagcatttat
     2761 gaaaattttt gatgtaaaag ccattggaca aggattgact gtattgccag gtctgactat
     2821 tgctaatcca gttcttcctt atatatttgg taatctcata gcattcattt taccaatcat
     2881 ttttattctc gcttatgata aatataaagt gtttatccct gaagctaaac accgtaaatt
     2941 tgcagaaaaa actgactcaa aaaaattgcc agttcaagta aagtgagatg agagcatgtt
     3001 aaattttttt agaaaaaatg aaataagcgt tgtagcacct gcaaccggtg tttttgaaaa
     3061 actggagaaa ttaagtgatc cagtcttttc aaaaggaatg atgggacaag gatttgcaat
     3121 tgatgcaaaa aacgaaacga ttgtatctcc aatcgatggt tttgtttcaa gtatttttcc
     3181 tacaaaacat gccttaggaa taaaaacaaa atcaggaatt gagatacttg ttcatgtagg
     3241 aatagataca gttgagctaa atggtgaggg atttgatatt aaagttcaag aaggacaaaa
     3301 agttaaggct ggggatattc ttatgaccat taattttgta gtttttgaaa agaatcacaa
     3361 gactaaagat gtcattgttg tttttccaga ctaccaagac gaaattttca ttccgaaact
     3421 tgaagaagta aaacaaggac aagaattgtt tgttcttggc taatgataag taattaataa
     3481 tagaatcgag gaaaatgtat ggtttttggg gctattgaag ctggtggaac aaaatttgtt
     3541 tgtgccattg gagataaaga tttgaacata tttgaaaaag tcagctttcc cacgactaca
     3601 cccaaagaaa caatgaattt agttttagat ttttttaatc atcataaaga tgatttagaa
     3661 ggtatcggag tgggatcgtt cggtcctatt gatattcatt gtgaatctaa aacgtatggc
     3721 ttcattacat caacgccaaa acttgcttgg caggattttg attttattgg aagtttaaaa
     3781 aagcaactta atattccaac tgcttggacg actgatgtaa atgcctcttg ctatggtgaa
     3841 tatgtttctg gaaatggcag aggcctttca agtgttgttt actataccgt aggaacgggc
     3901 ataggaggtg gcgctatcca aaacggaaac ttcattgaag gttttagtca tccagaaatg
     3961 ggacatatgc ttgtaaaacg acacaataaa gacaactttt ctgggacatg tccattccac
     4021 ggagactgtt tagagggctt agctgctggt ccggcaatcg aaagaagatc agggaaaaag
     4081 ggacaagaag tgactgaaaa tgacgttact tgggaaatag aagcttatta cattgctcag
     4141 tgtgcatata atacaacatt gatgttatca cctgatgtca ttatatttgg tggcggagta
     4201 atgaaacaaa aacacttaaa aaagaaagta caagataagt ttattgagtt gatcaacgga
     4261 tatgtcgcta ttcccgatgt tgacaattat attatgacac caaaattgtt agataattct
     4321 ggtattgtag gttgtttagc tttagcaaga gacgttaaag aaaaatctta atttggaaaa
     4381 aagatcgtct cgactatttg tttggttgat gcgatctttt ttttcaatta gtaattgaag
     4441 ggacgcctaa aagtgaaaaa aaaaatgaaa gatgaagtat tagagtatat caaaaataat
     4501 ttaaagtatt acgatttttc tgctcaagat attgcaatga aattttgtat taaaagaaat
     4561 gtcgcttccc actatttgaa tcagttattt tcagatggaa aattactaaa gaatgattct
     4621 gttagaccag taatgttcaa gtataatcaa caaaagccaa aagactgctt ttctaagttt
     4681 attggagcag acatatcttt gaaatccact atagataaat gcaaagctac tgtaatgtat
     4741 ccccctaatg gcctcccatt aataataaaa ggaaacagtg gcgttggaaa aagtttttta
     4801 gcaagtttga tctatcagta tgctcttgat agaaaagtaa ttcataatga cgcaaaattt
     4861 gttgttgtga attgtgcaga ttatgcaaat aatccagagc tattgtcagc agttttattt
     4921 ggatataaaa aaggagcctt tactggggct gaaaaagata ctgcagggat gctatcttct
     4981 gctaatggag gatacctttt tttagatgag gttcacaatc taagtgcaga aaaccaagag
     5041 aaattgtttc ttcttatgga ttctcaaaag tatcgtaaac ttggtgaaag tgtgcaatgg
     5101 gagtatgcaa atgttcgcct tatcttagcc acaacagaag ataaaaatac ctctttgtta
     5161 gcaactttta gaagacgcat cccagctgaa atcactctgc cagattacaa taatagaagt
     5221 acatctgaaa aaattcaatt attatttgaa tttcttaaag atgaagcaat tacaattgat
     5281 tcaaaaatat tttgttcgat tgaacttttt actgatcttt tatcgaaaac atttgaagga
     5341 aatgttggag aattgaggaa tgaaatcaag cttctttgtg cagaaggtta tttaggaaat
     5401 aagagaaagt cttcaattta tttaggggac aaactagaca gcggtttttg gatagatcca
     5461 gaaattagta tagatttaaa aaaaatgttt cttaaatcac tttctgaaat tgatttgatt
     5521 gaactttttc aaaatcacat ttcaattgaa gaaagcatga gtcaattaag agatagaata
     5581 cttaaagagt gtgcaggtt
//
LOCUS       DBKOIJ010000160         5481 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000160 DBKOIJ010000000
VERSION     DBKOIJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.164"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACV5NJ_002449"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACV5NJ_002449"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACV5NJ_002450"
     CDS             complement(580..1353)
                     /locus_tag="ACV5NJ_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHY6486486.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACV5NJ_002451"
     CDS             1497..1847
                     /locus_tag="ACV5NJ_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HHY6486487.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACV5NJ_002452"
     CDS             1828..2544
                     /locus_tag="ACV5NJ_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HHY6486488.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACV5NJ_002453"
     CDS             2544..3992
                     /locus_tag="ACV5NJ_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HHY6486489.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACV5NJ_002454"
     CDS             complement(4062..4571)
                     /locus_tag="ACV5NJ_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486490.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACV5NJ_002455"
     CDS             complement(4561..5286)
                     /locus_tag="ACV5NJ_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486491.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DBKOIJ010000161         5405 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000161 DBKOIJ010000000
VERSION     DBKOIJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.165"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(168..626)
                     /locus_tag="ACV5NJ_002456"
     CDS             complement(168..626)
                     /locus_tag="ACV5NJ_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HHY6486492.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(639..1970)
                     /locus_tag="ACV5NJ_002457"
     CDS             complement(639..1970)
                     /locus_tag="ACV5NJ_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HHY6486493.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1981..3423)
                     /locus_tag="ACV5NJ_002458"
     CDS             complement(1981..3423)
                     /locus_tag="ACV5NJ_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HHY6486494.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3410..4174)
                     /locus_tag="ACV5NJ_002459"
     CDS             complement(3410..4174)
                     /locus_tag="ACV5NJ_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HHY6486495.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4278..5153)
                     /locus_tag="ACV5NJ_002460"
     CDS             complement(4278..5153)
                     /locus_tag="ACV5NJ_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HHY6486496.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1672 a         1122 c          779 g         1832 t
ORIGIN      
        1 ttttaagaat cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat
       61 taacaaaaat caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg
      121 taaattcacc cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa
      181 caactatctt taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct
      241 ggagaaacta caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata
      301 atagctaaca gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga
      361 cttccttcga gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca
      421 ctttcgatcg ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc
      481 gcatggatat atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa
      541 aagacctcgt cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga
      601 atgtttcttg ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat
      661 tttttgttta ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg
      721 gtttgattca atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct
      781 actagtaact tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt
      841 ggttttctta gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca
      901 actgcatctg accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga
      961 cgacgatcat cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca
     1021 aaattaaagt tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg
     1081 gtatttttct ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc
     1141 tcaacgacca tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga
     1201 aaatttttcg tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata
     1261 ttaaaatcgg cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt
     1321 tctcgttgca attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa
     1381 tacgtaacga tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt
     1441 gaaatagtca tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct
     1501 tcagaaacga tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct
     1561 tgtatatgat cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct
     1621 ccgagtccac acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt
     1681 tctcgcatct ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa
     1741 gcaagctttg gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc
     1801 ataaatttaa caatcaagta catatccata tttctttctt catcaatatc atataaacgt
     1861 atttcagata atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta
     1921 tacgtactcc cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt
     1981 ctattcttct aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat
     2041 aatttgataa tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg
     2101 ctgttcctcg accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata
     2161 gacatccagg atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc
     2221 ataattttta cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc
     2281 tgccttagct tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt
     2341 aacgtaatac aaagcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc
     2401 gattcgaaaa ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat
     2461 attgaataag taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa
     2521 cataggagaa agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga
     2581 agttcctgca ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc
     2641 tttatagata gctagacata tagctggtat accaaacatc attactacaa aaaaaactga
     2701 taaaaacagt ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac
     2761 ttccttgcta ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg
     2821 gtgcagtcca aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc
     2881 tcccatatca acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat
     2941 aaagcccaaa aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt
     3001 ttcgcccgca aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt
     3061 ccatgccgct actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc
     3121 cataatttct aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat
     3181 tcctttgtcc ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa
     3241 gtttccaaaa acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc
     3301 tgataaacgc atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct
     3361 tccaagcttc tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct
     3421 catatgttac taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa
     3481 ttaaaaattc gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct
     3541 gatccaaata cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga
     3601 gtaaacttgc caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt
     3661 ctcctacgac atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt
     3721 caaagttgct ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat
     3781 tttcatccaa agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat
     3841 gataaatttt ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa
     3901 atggatgctg ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag
     3961 ttttgaggag tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca
     4021 tttcctttct tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg
     4081 atgtcaaaat tcctcgctga taagcatcca taatcccata attcacacca tggctatagc
     4141 caaaatcatc cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa
     4201 aataggcgca accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag
     4261 atagttacgc ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca
     4321 ttttcttgaa tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta
     4381 caatacgctt tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt
     4441 tcttcctgat aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa
     4501 gtcagtgatt ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg
     4561 gtcgtcattt ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta
     4621 atgatatgca tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga
     4681 ttgattcgct cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt
     4741 tctggcaaca taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa
     4801 atatgatctg tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt
     4861 atcagttcaa tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc
     4921 tcactgaatc gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca
     4981 acatcaaatg gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt
     5041 tctattccat cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc
     5101 gttaattcac ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt
     5161 tttgtctcca aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt
     5221 cgtgcctaat tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa
     5281 aagcgttatc aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg
     5341 aggtaattac ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg
     5401 ataat
//
LOCUS       DBKOIJ010000162         5388 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000162 DBKOIJ010000000
VERSION     DBKOIJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.166"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1089
                     /locus_tag="ACV5NJ_002461"
     CDS             <1..1089
                     /locus_tag="ACV5NJ_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486497.1"
                     /translation="VLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNWEGYENKNP
                     YVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKSAETK
                     PLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLN
                     DLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLL
                     YGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQ
                     FFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTK
                     IYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1310..3073
                     /locus_tag="ACV5NJ_002462"
     CDS             1310..3073
                     /locus_tag="ACV5NJ_002462"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HHY6486498.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3157..3615
                     /locus_tag="ACV5NJ_002463"
     CDS             3157..3615
                     /locus_tag="ACV5NJ_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HHY6486499.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3724..4407
                     /locus_tag="ACV5NJ_002464"
     CDS             3724..4407
                     /locus_tag="ACV5NJ_002464"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HHY6486500.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4554..5168
                     /locus_tag="ACV5NJ_002465"
     CDS             4554..5168
                     /locus_tag="ACV5NJ_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HHY6486501.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5184..>5388
                     /locus_tag="ACV5NJ_002466"
     CDS             5184..>5388
                     /locus_tag="ACV5NJ_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486502.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1915 a          845 c         1109 g         1519 t
ORIGIN      
        1 gtattggatt tttttaaaga aaatgattat ttgaaaaata aagaaatcat tatcccagaa
       61 tttgactcgc ggaacatctt gctagcttta gtacgttatg tcaattggga aggctatgaa
      121 aataaaaatc cgtatgtaga agaagcttgt aacgagttga ttgattatgt agagactcat
      181 ttttttaaaa gaagatattc ggaagatgaa aagtccttta tcatacgagg aatagagatc
      241 gcaatcggaa gaaaggcatt tccccttgga ctgaaagaag aagataagaa aagcgcagaa
      301 acgaagccgt tatttcaatt agtctgtcaa ggattggaag agcaaaaagg aattctagac
      361 ttacaagaag aagatgtcta ttatattttt tctttgttca acagtagaaa ttataccaat
      421 gagaacatgg agctgttaag aaaagatttc gaagttgttt ataaaaattt tgttctcaat
      481 gacctatcac tgaatgaatt gattttacag attattagta tcgctgaaat cgacgatgca
      541 gaaaatttta ttttcaagca atccttttta caatttgtcc gtacgttgtg ggcagatgga
      601 caagtatttt taccggaaag gatctatctt ctatcagaaa aagaacaact tctgtacgga
      661 aaaattgtag aaatattgga agagtggaaa aagaaaaatc aaatccaact aagatggaat
      721 gaaaatttca ttcgaaaatt cgtcaaaagt ctttctttga tcaacagtga acaagcgggg
      781 agacaggaaa tagaaatttt tgttgtttca gaaagttcag tcaaacaatt tttttatcga
      841 acccaatttc atttgtatgc ggaagaaggc atcgctgaaa taaatcctat tttatttcgt
      901 tcattagaag aattgcctga tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat
      961 gctgctagct atcaaaatgg attagaaact gaaaagacaa agatctatcc aatctcatta
     1021 aaaggtgcaa acatccattt gtatcatatt gctcaggaac ttcacctatt aaaaaaacat
     1081 atttcttgat gagaccttaa ttaggagacc ttctacaaag ctttttaatt gtctgtagaa
     1141 ggtttttatt tgagtcctga ttcgtgcgaa gatctactta tataacaagc tttctctgaa
     1201 atttttttaa aatgctattg acttagagtc aactctaagt agtagaatag ataaagtgta
     1261 acaaatctat tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt
     1321 attacatgta ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga
     1381 aatgaaacag ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg
     1441 tgggcacgtt ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt
     1501 tgattcacca aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct
     1561 tttaactggt cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc
     1621 caaccaacat gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag
     1681 cttagcaaca ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc
     1741 atttatcggt gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg
     1801 tgttttacaa tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa
     1861 tcatggcagt ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa
     1921 taacctattt aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga
     1981 aacggtgatc cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt
     2041 ccatacggaa aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg
     2101 gcatgtacca tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt
     2161 taatatgatc atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc
     2221 cgcagtcaat gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc
     2281 agatcattat tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt
     2341 ggcttctcag ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata
     2401 tgatcaatta tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg
     2461 cggaacgatc tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc
     2521 aagtatcccg aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct
     2581 agattgggcg ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt
     2641 agaacatggt acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa
     2701 tggaggaaaa gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt
     2761 aaaagctttt agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga
     2821 aaatgacgta gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga
     2881 agatggtagt ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc
     2941 aatgaacgta ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca
     3001 gctttatgaa cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt
     3061 gaatattcaa taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat
     3121 ggttttttca ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat
     3181 gtcaaaaagt tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca
     3241 tttggggata cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag
     3301 actggtacat caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt
     3361 ggtcaattag acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata
     3421 aatgtgacca tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa
     3481 ggggatcgga cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat
     3541 aaagaagcgt tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa
     3601 gtaattttct tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa
     3661 tagaaaaggg ttacattttt atatgaagct gtatttttat ataacattac tggaggagat
     3721 attgtggata cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa
     3781 aacgagccgc tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc
     3841 ggagcagttg gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt
     3901 gatttaccgg taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact
     3961 gcgacagaaa aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat
     4021 gcaacttctc gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac
     4081 gaacacggtc ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa
     4141 gaagatggtt ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag
     4201 acgcagacac ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta
     4261 attatggaag gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct
     4321 ttttcagtcg tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc
     4381 gaagccgcaa gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa
     4441 gaaaaattga tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca
     4501 ttttgtcttc aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg
     4561 aacggagtcc ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca
     4621 tccctagacc agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac
     4681 ccttcagtta cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga
     4741 gaaaacaaga tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta
     4801 tccatatcct tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta
     4861 atgaggaaag cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag
     4921 taccagcatt gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga
     4981 tacggaataa aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt
     5041 taaatgaaac agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg
     5101 taagccgttt ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc
     5161 ccgaataaaa aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa
     5221 aagaaatgaa aaaagaatat gaaacagtga atgattttct gcaagggcag tacttagaag
     5281 ttccgccatt gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaatag
     5341 aaatgccaga tcaaacgatt agcggattgt ttaattactt taacaaat
//
LOCUS       DBKOIJ010000163         5312 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000163 DBKOIJ010000000
VERSION     DBKOIJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.167"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACV5NJ_002467"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACV5NJ_002467"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HHY6486503.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="ACV5NJ_002468"
     CDS             complement(1298..2134)
                     /locus_tag="ACV5NJ_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486504.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="ACV5NJ_002469"
     CDS             complement(2318..3535)
                     /locus_tag="ACV5NJ_002469"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HHY6486505.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3863)
                     /locus_tag="ACV5NJ_002470"
     CDS             complement(3684..3863)
                     /locus_tag="ACV5NJ_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486506.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3918..4001
                     /locus_tag="ACV5NJ_002471"
     tRNA            3918..4001
                     /locus_tag="ACV5NJ_002471"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..5312)
                     /locus_tag="ACV5NJ_002472"
                     /pseudo
     CDS             complement(4241..5312)
                     /locus_tag="ACV5NJ_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac at
//
LOCUS       DBKOIJ010000164         5296 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000164 DBKOIJ010000000
VERSION     DBKOIJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.168"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            236..2254
                     /locus_tag="ACV5NJ_002473"
     CDS             236..2254
                     /locus_tag="ACV5NJ_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HHY6486507.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2324..2530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2681..5095
                     /gene="leuS"
                     /locus_tag="ACV5NJ_002474"
     CDS             2681..5095
                     /gene="leuS"
                     /locus_tag="ACV5NJ_002474"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HHY6486508.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         1826 a          968 c         1122 g         1380 t
ORIGIN      
        1 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
       61 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
      121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
      181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
      241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
      301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
      361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
      421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
      481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
      541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
      601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
      661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
      721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
      781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
      841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
      901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
      961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     1021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     1081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     1141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     1201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     1261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     1321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     1381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     1441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     1501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     1561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     1621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     1681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     1741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     1801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     1861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     1921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     1981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     2041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     2101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     2161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     2221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     2281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     2341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     2401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     2461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     2521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     2581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     2641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     2701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     2761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     2821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     2881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     2941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     3001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     3061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     3121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     3181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     3241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     3301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     3361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     3421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     3481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     3541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     3601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     3661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     3721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     3781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     3841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     3901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     3961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     4021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     4081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     4141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     4201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     4261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     4321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     4381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     4441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     4501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     4561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     4621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     4681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     4741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     4801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     4861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     4921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     4981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     5041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     5101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     5161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     5221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     5281 ctgtaatcat caaatt
//
LOCUS       DBKOIJ010000165         5294 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000165 DBKOIJ010000000
VERSION     DBKOIJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.169"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            4..861
                     /locus_tag="ACV5NJ_002475"
     CDS             4..861
                     /locus_tag="ACV5NJ_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HHY6486509.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            976..1896
                     /gene="pstC"
                     /locus_tag="ACV5NJ_002476"
     CDS             976..1896
                     /gene="pstC"
                     /locus_tag="ACV5NJ_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HHY6486510.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1896..2780
                     /gene="pstA"
                     /locus_tag="ACV5NJ_002477"
     CDS             1896..2780
                     /gene="pstA"
                     /locus_tag="ACV5NJ_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HHY6486511.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2795..3601
                     /gene="pstB"
                     /locus_tag="ACV5NJ_002478"
     CDS             2795..3601
                     /gene="pstB"
                     /locus_tag="ACV5NJ_002478"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HHY6486512.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3617..4375
                     /gene="pstB"
                     /locus_tag="ACV5NJ_002479"
     CDS             3617..4375
                     /gene="pstB"
                     /locus_tag="ACV5NJ_002479"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HHY6486513.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4388..5065
                     /gene="phoU"
                     /locus_tag="ACV5NJ_002480"
     CDS             4388..5065
                     /gene="phoU"
                     /locus_tag="ACV5NJ_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HHY6486514.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT         1663 a          949 c         1185 g         1497 t
ORIGIN      
        1 ctaatgaaaa aacttaaatt attcttacca ataataggat tagccattct tttatcaggc
       61 tgttcaaagt ggattgatcg aggagaatcc attacggctg ttggctcttc tgctttacag
      121 cctttagtag aaacagttgc tgaaacttac cagacagaaa atcctggaaa attcatcaat
      181 gtacaagggg gcggaagcgg aactggtttg tctcaagtac agtcaggagc agtcgacatc
      241 ggaaactctg atttgtttgc ggaagaaaaa tcggggatcg atgcctcagc attagtcgat
      301 catcgagtag cagttgttgg gatcactcct attgtgaaca aggatattgg cgtaaaagac
      361 atcagtatgg aaaacctgaa aaaactattt acaggcgaga tcaaaaactg gagtgaagtc
      421 ggtggaaaag atcagccagt agttatattg aaccgcgctt caggaagcgg aacccgtagc
      481 acgtttgaaa aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca
      541 agcggaatgg tgcgccagat cgttgccgac acacccggtg caatcagcta tgttgctttt
      601 tcttatgttt ccgatgatgt agatacgttg aatgttaacg gtgtagaacc gacagatgaa
      661 aatgtgacaa cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagatcat
      721 ccaaaaaaac tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataac
      781 atcgttggag aactaggtta tatccctgtt tctcatatga aagtcgaacg tgattggcaa
      841 ggaaatatcg ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca
      901 tgttattaaa accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt
      961 aagggggagt catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca
     1021 aaaatggaac agcgtggaaa attcatcagt tttttatgta tcgcgctgat cgtgttggtt
     1081 gtattatcta ttttttattt tgtagctagt aaaggattgg ccaccttttt tactgataag
     1141 atcaatgtat ttgattttct gtttggtaca gattggaatc caagccaaat cggagcaaat
     1201 gggaaaccaa tggtaggggc attgcctatg atcacaggtt cgtttattgt gaccttttta
     1261 tctgctatcg ttgccacgcc atttgccatt ggtgcagcag tattcatgac ggagatctct
     1321 cctaaaaacg gaacgaagat cttacagccg gtcatcgagc tgcttgtagg tattccttct
     1381 gtcgtttatg gttttatcgg tttatcagtt atcgtgcctt ttgtcagaag tatttttggc
     1441 gggacagggt ttggtatctt agctggtaca tttgtgttgt tcgttatgat cttgccgact
     1501 gtcacaacga tgactgttga tgcattaaag gcagtaccac ggcactatcg ggaagcttca
     1561 ttagcgcttg gtgccacaag atggcaaaca atctacaaag tcgtgctacg agcagccgtg
     1621 cccgggattt taacagcagt cgtttttggt atggctcgtg cgtttggtga agccttagct
     1681 atccaaatgg tcatcggtaa tgcagcattg atgccaacag gacttactac tcctgcttct
     1741 acattgacgt ccatcttaac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat
     1801 aatgtgttat ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg
     1861 atccgtatga ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa
     1921 ttgctacagg tgttttatat atagtttcgg ggatcatcgt attgatttta gcagctttac
     1981 tactttatat cttagttcgg ggaataccgc acatttcatg gtccttcttg accgaacctt
     2041 ccaaagccta ccaagaaggt ggtggaatcg ggatccagct atttaactcg atttatttgc
     2101 ttttgatcac gatgatcatc agttttccta tttctttagg tgcgggtatc tatctttcag
     2161 aatatgctgg aaaaaactgg ctaacagatg tgattcgcac gtcgattgaa atcttgagct
     2221 cgttgccttc tgtcgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt
     2281 atggtttctc gattatttct ggtgcattgg ctttgactgt attcaacttg cctcttttga
     2341 ctagaacagt tgaggagtct ttacaagcgg tccattacac acaacgagaa gcaggattgg
     2401 ccttaggttt gtcacgctgg gaaacagtga tgaaagtggt ggttccagaa gcgacaccag
     2461 ggattttgac tggtgtgatc ctaagttctg gaagaatttt tggtgaagca gctgcattga
     2521 tctatacggc tggacaaagt gcacctgcgt tagattttac gaactggaat ccattaagtg
     2581 tttcaagccc gatcagcatt ttccgtcaag ctgaaacatt agctgtccat atttggaaga
     2641 tcaatacaga aggcacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt
     2701 tgatcattgc tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc
     2761 gaaaaatgac ttcagcctaa ggagaagaag caaaatgaaa aaatataact ggaatgaaac
     2821 aaatgtcatc accatgccaa aagagaaaga agtagcacta tatacggatg atatacatgt
     2881 ctggtatggc gacaatgaag caatcaaagg agtggatctg gaatttgaaa aaaataagat
     2941 cactgcactg attggtcctt ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg
     3001 aatgaatgat gggatcgcta atacgaaaat cactgggaaa atcatgtatc aaggcgtaga
     3061 tattaatgcg cctcaagtgg atgtttatga gatgcgcaaa cggattggca tggtttttca
     3121 acgtcctaat ccgttcagca agtcgattta tgacaatatt acatttgctt tgaaacagca
     3181 cggcgaaaaa aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagctgc
     3241 cttgtgggac caagtaaaag acagcttgga caaaagtgcc ttggctttat ctggcgggca
     3301 acagcaacgt ttatgcattg ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga
     3361 cgagccggca agtgctttgg accctatttc gacaggaacg gttgaagaaa cacttgtaaa
     3421 cttgaaagaa gactatacga tcatcatcgt gacgcataac atgcaacaag cagctcgtat
     3481 cagcgactat actgcctttt tctatctagg aaaagtaatg gaatatgaca aaactaacaa
     3541 gatatttacc agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta
     3601 agacggaggc ctcaacatga caaaagaaat tatttcatct aaagatcttc atctgtatta
     3661 tggaaagaaa gaagcactaa aagggatcga tctaagtttc aatcaagggg aaatcactgc
     3721 gatgatcggt ccttctggtt gcggaaaatc cacttatctg cgttctttga accggatgaa
     3781 tgacctgatt ccgaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta
     3841 tggtccaaaa acagatgttg ttgaactaag aaaagaaatc ggcatggtat tccagcagcc
     3901 aaacccattt cctttttcta tctatgaaaa tgtcatctat ggattgaaat taaaagggga
     3961 aaaagataaa aaagtccttg atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg
     4021 ggatgatgtg aaagacaaat tgcataaaag tgctttatct ttatccggcg ggcagcagca
     4081 gcgtgtatgt atcgcacgtg ttttggcagt taatccggaa atcatcttat tggatgaacc
     4141 aacaagtgcg cttgatcctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag
     4201 agagcggtat acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga
     4261 taagacggca ttctttttag atggtcactt gatcgaatac aacgatacga agaagatatt
     4321 catgaatcca gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg
     4381 aaacggaatg ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta
     4441 cgaaatgggg atgatggtga gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca
     4501 cgataaaatc ttagcccaag aagttatcga aaacgatata aatatcaata acatggaaac
     4561 acgcttggag aagaaaagtt ttgaaatgat cgcattgcaa cagccagtta caactgattt
     4621 gcgtatgatc attacggtca tgaaagctag ctcggattta gaacgtatgg ccgatcatgc
     4681 ggtgtctgtt gctaaatcca cgattcgcgt aaaagggcag acgagaatac cggaaatcga
     4741 aaaagagatc tccgatatgt ccgattatgt gaaaaaaatg gtggacaacg tcctagttgc
     4801 ttatgtcaaa acagacgaaa aagacgcacg tacgatcgcc aatatggatc aaagagtcaa
     4861 tgactatttc aatcgaatct ataatgcaac aatcaaaaat atgcaggaaa atcctgagac
     4921 agtcatcagt ggaacagact acttgaatgt tgccagttat ctagaacgga tcggagacta
     4981 tgtgacaaac atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgagttaaa
     5041 tacgaaccac aatgagattt tctaaactga agctgtgatt atgccgtttg tttatgaaaa
     5101 gaggttgtga aaaagctgtt ctacatcaag taataagaac agtcaaacaa aaaatagctc
     5161 ctcatatttt tcgtttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
     5221 ttcgggaaag ggtttgtgac aaaacatgtc acaaaccctt ttcatcattt catagaaagg
     5281 ataaagaatg caaa
//
LOCUS       DBKOIJ010000166         5241 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000166 DBKOIJ010000000
VERSION     DBKOIJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.170"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..241)
                     /gene="prgO"
                     /locus_tag="ACV5NJ_002481"
     CDS             complement(<1..241)
                     /gene="prgO"
                     /locus_tag="ACV5NJ_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HHY6486515.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDE"
     gene            complement(213..1166)
                     /locus_tag="ACV5NJ_002482"
     CDS             complement(213..1166)
                     /locus_tag="ACV5NJ_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HHY6486516.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1778..3271
                     /locus_tag="ACV5NJ_002483"
     CDS             1778..3271
                     /locus_tag="ACV5NJ_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HHY6486517.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3406..3702
                     /locus_tag="ACV5NJ_002484"
     CDS             3406..3702
                     /locus_tag="ACV5NJ_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HHY6486518.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3803..4654
                     /locus_tag="ACV5NJ_002485"
     CDS             3803..4654
                     /locus_tag="ACV5NJ_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486519.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4675..4887
                     /locus_tag="ACV5NJ_002486"
     CDS             4675..4887
                     /locus_tag="ACV5NJ_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486520.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1791 a          816 c          994 g         1640 t
ORIGIN      
        1 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
       61 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
      121 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
      181 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
      241 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
      301 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
      361 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
      421 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
      481 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
      541 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
      601 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
      661 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
      721 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
      781 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
      841 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
      901 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
      961 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     1021 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     1081 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     1141 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     1201 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     1261 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     1321 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     1381 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     1441 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     1501 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     1561 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     1621 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     1681 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     1741 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     1801 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     1861 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     1921 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     1981 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     2041 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     2101 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     2161 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     2221 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     2281 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     2341 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     2401 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     2461 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     2521 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     2581 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     2641 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     2701 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     2761 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     2821 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     2881 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     2941 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     3001 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     3061 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
     3121 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
     3181 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
     3241 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccctataca
     3301 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
     3361 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
     3421 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
     3481 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
     3541 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
     3601 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
     3661 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
     3721 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
     3781 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca cctggcttcc
     3841 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
     3901 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
     3961 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
     4021 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
     4081 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
     4141 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
     4201 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
     4261 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
     4321 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
     4381 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
     4441 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
     4501 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
     4561 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
     4621 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
     4681 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
     4741 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
     4801 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
     4861 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
     4921 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
     4981 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
     5041 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
     5101 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
     5161 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
     5221 gaaattgatc gaagatgtga c
//
LOCUS       DBKOIJ010000167         5202 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000167 DBKOIJ010000000
VERSION     DBKOIJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.171"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(387..761)
                     /locus_tag="ACV5NJ_002487"
     CDS             complement(387..761)
                     /locus_tag="ACV5NJ_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHY6486521.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(784..2184)
                     /locus_tag="ACV5NJ_002488"
     CDS             complement(784..2184)
                     /locus_tag="ACV5NJ_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HHY6486522.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2188..2598)
                     /locus_tag="ACV5NJ_002489"
     CDS             complement(2188..2598)
                     /locus_tag="ACV5NJ_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6486523.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2616..3107)
                     /locus_tag="ACV5NJ_002490"
     CDS             complement(2616..3107)
                     /locus_tag="ACV5NJ_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HHY6486524.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3117..3908)
                     /locus_tag="ACV5NJ_002491"
     CDS             complement(3117..3908)
                     /locus_tag="ACV5NJ_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHY6486525.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3901..4674)
                     /locus_tag="ACV5NJ_002492"
     CDS             complement(3901..4674)
                     /locus_tag="ACV5NJ_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HHY6486526.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4830..>5202)
                     /locus_tag="ACV5NJ_002493"
     CDS             complement(4830..>5202)
                     /locus_tag="ACV5NJ_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HHY6486527.1"
                     /translation="EFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYD
                     VKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIY
                     CIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1665 a         1049 c          683 g         1805 t
ORIGIN      
        1 gaaaaatact aaaaattttt caaaataaaa aaaggcatac agacaataaa ctgtctatac
       61 gcctcacaga ctctattcta tacatctaat tttggctgtc ctgcaaaaaa tatgtgttag
      121 cctccatcga aagatcaaac tttggaaaac atcattataa ctttgcttgg taagagcttt
      181 gctaaaacat gatatgactt gtatacaaaa tgtggagtgt attccaataa ataggaagga
      241 ttacaaaatt tgaaaccaaa taagtaaaaa atactgtcac tttgaattaa tttgaaatag
      301 taaattctca ttaaactatt gaaatgctga taccaaaaaa tgaagtactt ttcgttcata
      361 ccaacttaga gcacttcgtt atctatttat ttcttcattt ggcttatctc tataatctca
      421 tgatttggtc cgtagaaatt aaagtaacgg attccatttt cccagaacgg taattccata
      481 atatttttat ctaataattc atatcctaaa tcttgaattg aattgaatac ctcatctatg
      541 tcatccgtat ctaatgctag atgatcaatg gctcctctac actcagtagt tttatcttcc
      601 catatttcta ttatcgttcc attcaactcg aaaaagccga ctttacttcc cacattcatt
      661 tcttcattaa tcagactaaa ccctaaggat tcataaaaga tttttgaagc tccaaaatct
      721 tttgttggaa ttccaatatg ttgtaaacca tttaatttca cttacatcat cccttcaatt
      781 gttttaaaat ccacattcat aatttattga atagtctagt ttatctacct tgattgctcc
      841 ctccgtaaca gtcatactta atgctgttcc gactttatca tacattctag tacttaaagc
      901 aacattatca atatataaaa cagctatcgt atcatcaaca attaacttta gttcatattc
      961 tttgcctatt tccaaaaata gtggtcgttg cagcccctta tcaaacattt gaggccaagg
     1021 atagttcgga gtttttccga aagtaagctg attattaatc aatgaaatac gaaattcgta
     1081 tccttcttga gtatcactat ctacaaataa ttttaaagca aactcctttg ttaacgatgt
     1141 aatagtcaaa gttgtttcaa atagaaaata atcttcgatt ttaggaacaa ttactctttc
     1201 tttttttcca aattctttct ctaacttaaa acttgaaaat ggagtgattt ctttaaaatt
     1261 ctcaattatt ttttcaggca gtttaacacc aagagttttg tctgtctttt gaataatttg
     1321 atgtggaaca agtgcaccgc cccaatccca gttattttga tcgttctccc cttcttttgt
     1381 tgccacccag ccagttagaa aacggccagt ttcattagtt ccgactgttc ttgctgcata
     1441 ataggctttt ccgtcgaaag cttcgtctac tggtgatttc caatctgaaa ataatcctgc
     1501 tccgactcga tatttagtct ttttatcttc acaatactct gaaaaaagta aataccacgt
     1561 tccatcaatt tcaaatatat caggcatttc aatcatgtta aattcattat tagcccagta
     1621 gtctcctttg aaattccaat cttttaaatc ttcggattca aaatagacca atctccccgt
     1681 cggctttttc ttacttgctg gttttctagc tcctagtatc aaaacgtatt ttccatgatc
     1741 ttgatcaaaa attgcaaaag gatcgcgcca atcattctta tcatatcctt cttgaggaac
     1801 aagttttgct gcttcgccat cctttttcca atgaattaaa tctttagaac ttgcaatcat
     1861 taacacttct gaagtttttc catccaactt agctttgcga ttatgtccag tatacaaagc
     1921 taaatactca ttgattcctt ggacaatact tccagcgtaa atgtattgat caatgtcatc
     1981 gttgccacca cgatgaatta cttccccgta ttcttcaaat ttcacaaaat cagtggttgt
     2041 aactagtgac cacccaaaag gatcagttaa aggtttagga tttctcgtat ctcgttgatg
     2101 aaatagataa aaagttccat ctttataaaa aggcatgcaa tccccaaacc aaaaatcttt
     2161 aggcttgtaa aaaatttttt gcatttatca ctcatcctct tcgttagtat gtctttgcat
     2221 atctgcaaga cacgcaattg tattcatatt tcttagattt tcgatgatat catcgctttt
     2281 ttttccttgt aatctcaaat tgacagcctc aatcaaaatt cctaaattca tgccaccaag
     2341 aagttgaaac ccattttgaa atacttcact tcttttagca aattcgttga aaggtgttcc
     2401 accaatcaag tcagcaaata ccataacttc ttgatttttt acttgagaaa ctattttttc
     2461 aatgttttca cggaattctt caatgcctgc atctccaagt gttaccacat gacaatttaa
     2521 attatcgcca aaaaacattt ttaatgtatc tacaaatcca atggcaatat ttccatgaga
     2581 tgtaattata aactccatcc actcatcctc tgttcttaat aaataaaatc ctttaaaaat
     2641 tcttttgaat ctctaggagt ttgtatcact gtcacttcaa ctccatcact aattagttct
     2701 ttaaaagcct ctctctcttc ttcagtaata tttatcgtcg gacggatgcg ttctgttcca
     2761 ttccttcttg ataaatttcc tacgtttaca cttgttattg gtaaaccgtt atcaatcaaa
     2821 aatttaacat cgattggaga aactgcaaca ataagaactt tttgtccttc atatttacca
     2881 gaaagaatat ttttaactgc tacttccctt gtaattattg aagttctaac attaggtggt
     2941 gcagcgaggc gaactacagc tttttttacg tcatcattgg ctacttcatt attaataacc
     3001 ataattctgt tagcactcaa ttgatttgtc cattgtgttg ctacttgtcc atgaatcatt
     3061 cgatcatcaa tacgaacatg tataatacct ttcatttttt catccatatt caacacctac
     3121 ttcaaaatat ttaacgctct taacgcaata gaaactacta agattaatag aattacataa
     3181 actggtttaa cctttcgtcc taacaaataa tacacaattg ctactacaca tgcaggtaat
     3241 aaaccaggca taacttgatc aaagatttca tttccggtca ttgttaagtc gccttgttta
     3301 aattcaaagc caacattcac ttttacaact gtggcaatga gtgcaccgac caccattaac
     3361 cctaaaattg atactgcatc agtaatagac tttaattttg tttcaatttg agttagtagg
     3421 ctcgcacccg attctttccc agcaaggtat aaaggataag cagtaatcct taataaaatt
     3481 gatgccagta tccacaaaat aattccaaca aaactacctt ccaatgccat attagctgca
     3541 attgctccaa aaatagtcca tggaataact acaaatacac tatctccaat tcccgctaaa
     3601 ggacccatta gacctgtttt cagagcactt actgcttcca aagacttatt tttttcttct
     3661 ttttcaattc caacattcat accaataatg taaggcgctg tatacggcgt agtgttaaaa
     3721 aattgtaggt gagctaaaac ggattgtttc attccttcag gatcatcttt atatgtgtcc
     3781 ttgataaaag gaagcataga atacgcataa cccaaccctt ccattttttc ataatttaat
     3841 gaagttgcac cattgagaat ccaccggaaa attacttgtt tgtcgatttt tgtcagtttc
     3901 ttattcatct tcgtcaaaac ctcctgttgc agctggtgct acttgagtat tatttgatct
     3961 atttttatat acgattagtg cgattgcagc gccaagtaac gaaatcccta aaattggtat
     4021 ttggaaataa actgccaaaa cgaaaccaac taacaaatag gcaaagtaat cttttgtagg
     4081 taaatattta agtagcattc ctatcccaac agcaggtaat agtccccctg ctacctttaa
     4141 accaccggta acccattcag gaatataatt gattgcactt cccactaacg ttgggcctaa
     4201 tatcacagtt aacaaaacag gaattccaga tacaaagctt gtattaattg atgccaaata
     4261 ttgcatccac tctaattttt gataatcttc tttttgtgcg aatttttctg ccttttgttg
     4321 aaaaaaaatg tttgttgacc actttagaac atctagttgt acaattaaca ttgctaccgg
     4381 tattgctaaa gttattccta tagaagcatc ctggttagtt gagattgcta aataagtacc
     4441 gatcaatgct gctgtttgat aatccggtac tgatgcacca ccaaaactag taatacctag
     4501 tgtcattaat tgcaaagtcc caccgatata aagtccagtt tgaatatctc ccataataag
     4561 tccggtcaag aaacctgcaa cgacaggttg ggaggttccg attttgggcc cttccttatc
     4621 ccagttaatg aaaaatccat acaacacaac taaaaatgtt tgccaaaacg ccattttgtt
     4681 ctctcctttc ttcttatact tttatataag caattactgt gccaactaac attatccaat
     4741 aaagcgggaa aatacagtaa atattaatta aatgagcgtc taaatatact ttggacaccc
     4801 tttcattttt ttagacgcta gtatgctatt tagaaaatag tctgctcaaa aattgtatag
     4861 attaactcat actctccttt gggtatttca atacagtaaa tatctttgat ttctgaaaaa
     4921 aaatcttttg atacatttaa aaaagtatca aatagttgcg ctccctcttt caaatgttca
     4981 tttgttattc catcaccaat cataattcgt tcaatcattc cagaaagatg tagaaataga
     5041 ttaattctca gaaaacttgg gattttgaca tcgtaatatt tttcatacaa atatagtact
     5101 ttttctatct cattgaatac ttttttggaa tctaaaaagc gaagcttagt actagcgcct
     5161 tcaatagtaa agagtttaac taattcattt aagcagaact ca
//
LOCUS       DBKOIJ010000168         5113 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000168 DBKOIJ010000000
VERSION     DBKOIJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.172"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            93..1262
                     /locus_tag="ACV5NJ_002494"
     CDS             93..1262
                     /locus_tag="ACV5NJ_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HHY6486528.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1388..2467)
                     /locus_tag="ACV5NJ_002495"
     CDS             complement(1388..2467)
                     /locus_tag="ACV5NJ_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HHY6486529.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2576..3016)
                     /locus_tag="ACV5NJ_002496"
     CDS             complement(2576..3016)
                     /locus_tag="ACV5NJ_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHY6486530.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3073..3483)
                     /locus_tag="ACV5NJ_002497"
     CDS             complement(3073..3483)
                     /locus_tag="ACV5NJ_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HHY6486531.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3664..4098)
                     /locus_tag="ACV5NJ_002498"
     CDS             complement(3664..4098)
                     /locus_tag="ACV5NJ_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HHY6486532.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4198..5046)
                     /locus_tag="ACV5NJ_002499"
     CDS             complement(4198..5046)
                     /locus_tag="ACV5NJ_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HHY6486533.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1870 a          981 c          855 g         1407 t
ORIGIN      
        1 tcataaattt agacaaaaaa acagtaaaca tataggtaga atctctacta tcttatttct
       61 tgcatacaaa aaaatcaagg agtgagagag ttatgagata ttcaaccatc ttaatatcaa
      121 catttgcagc cttaagctta tcgcttacta taaatcagac agtccacaca gaggagatag
      181 aaacagccaa atggacagaa accaagtggg ccaacgtcaa tcgaattacc tttcaggatg
      241 aattttcatc cttcaattca ggagtcgcaa aattaagaaa aggacaaaca gcgaccatca
      301 aagcaaaaat tgactattca ggagacttag cacctatact acaatcagct gttacatttg
      361 agaatgttga tcccgctttg tcgattggct atgacgagaa cactttgact atccaacaca
      421 agcaagcgat atttaccttc accgttacat taaatcagga cttgttaaaa ccagcgcttt
      481 ttactgtcaa ggtatcagat ggactaccga acgatcatca tcatttaacg ccgtatagtc
      541 agcggcagac aattgagcaa gattcagcta tcgactcagg aggagatctt gttgaagaac
      601 caacagataa accagaaaat gaaaataagc cagaagtacc gcctacagaa aatccagacg
      661 gagaacagaa accggaaata gaacctggag aagaacccga tacagaaact caaccagagc
      721 cagacaatga atcaaaacct gaaataaccc ctggagaaaa accagatgta gacccagaag
      781 aaaaaccgga tgtgactcca gaaccagata cggattctgg aaaccaaaca gtaccggaaa
      841 caaatccaga cacagataat gaaactgaaa atccagaaaa actagaggta gatccagaag
      901 aaaaaccaga tgtgactcca gaaccagata cggacgcaag agaccaagga ataccagaaa
      961 aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa tcaaatctag
     1021 caattctaaa aataaatgaa gagcagctga ataaaaaatc acgaatattt gatagcgccc
     1081 aatcagctga aaccttaaaa agtagcaagg atacaacctt tgctagtcct gaaacgaaaa
     1141 ataaacagct accaaaatcc ggagagtcac aaaacaaagt tatactgtgg agcggaatca
     1201 ttctattaag tatagcaaca atgctttctg caaaaagatt taaacaaaac agatctctct
     1261 aacatttgaa taatgtctgt gaacgcattt ttcaaataaa aagaaatatc cagaaaacga
     1321 ccccaaaata ccagattgaa atctgatatt tggggtcgtt catactttat ctttcttcat
     1381 atttatttta agggatatct gtttcttttg gaagatggtt cgggatcttt ttcactttca
     1441 atacatctaa aaagaaggta aagatcggaa tgccgacaat caagccccaa acgccaaaga
     1501 accgttcgct aacaagtaag accacaaatg tataaaaaat cggcagttct gttttgctgg
     1561 acatcaactt cggattcagc acataagact caattagatg gacaatcaaa atcgtaatca
     1621 ggatataaac aacatctcgg atgcctcctt gggaataagc tatgaaagat agaggaatac
     1681 aagaaatgat aacacctgct actgggatca gacttaaaat gaaaatcatg atcgctaaac
     1741 tcagcagctg atgaaagcca aatacagcca aggcgattgt agtgagcaac gtgtttaaaa
     1801 gagcgataac aaattgagct tctaatacca aaccaaatgt attgacaaac ttatcggcaa
     1861 aatagtaaat atcttgaaag aaccaagaaa attcactttt taaaaacaat cttgagaata
     1921 caattgtttt tttcttttca atcataaaaa agaaacttaa gatgaatgac atagcaaacg
     1981 ccactgccat ttttcctata tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa
     2041 gctgttcaaa aaaatcgttt cgctcaatat aagtatggat atattgtaaa acaggatctg
     2101 catcttttgg ctgatgctcg taaaaatcaa taacggactg gatcatatca aatgtttgat
     2161 gaataagcat cgggatatac tttgtgaatg ccagatagat aaggtaaacg atcagtgtat
     2221 aaatcatgat agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa
     2281 gatgaatcat caaataagta aagataaatg tgagcaaaat ggttgtaatc atacttctaa
     2341 ctaagaaaag aacaaaaacg acaaataata aaacagtgag acggcgtatt ttttcattcg
     2401 ccaaaaaacg ctgataaacg gtcaaatcga caacctccaa ataacaaaaa ataaacacgt
     2461 tattcattat atgatggaat atgaaaataa gaaagagaaa gagcaaaaag aaaaaccgct
     2521 aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc
     2581 cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat
     2641 ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt
     2701 gtttttgcaa cgcagcacct tgaggagtca gcgtaacatt tacgattctt tcatcacccg
     2761 tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg
     2821 taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc
     2881 ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg aggagggaag
     2941 tgtagtgtct aatgatttcc ttagaggcgg catataaagg aaaacatact tgattaacta
     3001 aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt
     3061 acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc
     3121 ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga
     3181 aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt cactttctga
     3241 ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat
     3301 caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt
     3361 ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg
     3421 attatgaact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt
     3481 cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg
     3541 gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc
     3601 tttctcgaat agcaaacata tacaaaaaaa tatcagagac aaaaactaat ctcatcataa
     3661 acattacata tatttctcaa aaagttgttt tctttcttca aaacttactt cacgatggac
     3721 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     3781 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     3841 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     3901 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg tgccatacaa
     3961 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     4021 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatctat
     4081 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     4141 tttattatac cagttaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     4201 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     4261 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     4321 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     4381 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     4441 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     4501 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaacaaacag
     4561 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     4621 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     4681 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     4741 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaaaca agcttccgaa
     4801 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     4861 attcaacact aaaataacta tagctcggtc aaatcctaaa acagcttcca gtaatattcc
     4921 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     4981 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     5041 tttcatttat ccacttcctt aattcattaa taatactctt atcttattat aacttacttt
     5101 tcaccttaag tta
//
LOCUS       DBKOIJ010000169         4965 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000169 DBKOIJ010000000
VERSION     DBKOIJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.173"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(44..955)
                     /locus_tag="ACV5NJ_002500"
     CDS             complement(44..955)
                     /locus_tag="ACV5NJ_002500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHY6486534.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1332..1970)
                     /locus_tag="ACV5NJ_002501"
     CDS             complement(1332..1970)
                     /locus_tag="ACV5NJ_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486535.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1974..3956)
                     /locus_tag="ACV5NJ_002502"
                     /pseudo
     CDS             complement(1974..3956)
                     /locus_tag="ACV5NJ_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4136..4801
                     /locus_tag="ACV5NJ_002503"
     CDS             4136..4801
                     /locus_tag="ACV5NJ_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486536.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
BASE COUNT         1580 a          913 c          769 g         1703 t
ORIGIN      
        1 agttttattg atttaaatag ttcatatttt ttatttatac cgcttattta ctacttgctg
       61 tactagaatc tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta
      121 cattagcgtc tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt
      181 ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt
      241 tttggttttt caccatttta accacataat attccgtagt ataagttgaa gcattggtag
      301 aagtgatcac atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg
      361 ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag
      421 tatcagttga cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct
      481 tcacatcttt tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat
      541 ggaaagattc ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac
      601 cttcttgaaa ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg
      661 cagatttcaa ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattggg
      721 aatcgatttc tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga
      781 tcaactgttg cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt
      841 gttcgctgat tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc
      901 ctgacaataa gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc
      961 aattctccta cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat
     1021 catcaagaac acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa
     1081 tccagaaagt tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac
     1141 ttcttttgtt accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt
     1201 agaacaaatg ttatcgattt tgtttttctt cttccgctgt tgggatattc aaatcagaca
     1261 cttttatgtt cgattttcca gacatttttt tgttgttttt attagaaaaa gtttgttttt
     1321 tcatttgttt tctaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc
     1381 gtgaccacga taactaacgt accttgggaa gcctgttgcg ataagatttc aacgacttgc
     1441 tccttatttc gtttatctaa tgatccagtt ggttcatcgg ctaaaatgat tttgctattc
     1501 ttcaataaga ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata
     1561 ggcgtactta gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc
     1621 ttatctgaaa catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt
     1681 tcatattcaa tcaacccaaa atcttgaaaa agaaaagaga gggcatttct ttttatcaag
     1741 cgcttctctc ttccagttaa atgttttgtt tcttgattaa ataaataaaa ttgccctgaa
     1801 ctgaattgat cattcaatcc taatatattc aataaagtac ttttccctgc tccgctctct
     1861 ccaactacag tcacaaaatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt
     1921 ttgtttcgaa tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct
     1981 ccattcatca gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga
     2041 ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata
     2101 agaaaaagga agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct
     2161 ttatacgttt caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg
     2221 tagacaaaat atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt
     2281 aaaaatatct gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca
     2341 gtctgagtat acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc
     2401 tcaaataacc tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct
     2461 ccgctgagaa tatttctttc ccaatacgta ctgtttttca atgtcagtat ttcaacaacc
     2521 ggataatgtt cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt
     2581 ataaatagaa atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc
     2641 aggtcatttt ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt
     2701 tctttttcgg ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga
     2761 taattcagat ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct
     2821 gatacattga ctaatatact tccgttttta ttcaaagttt catataattc ctgatcggct
     2881 gctgatgcaa aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga
     2941 taaaatacat gatagtcctc accagcgatt tcttttggtg tttcatggga aacagctaac
     3001 tgtgaagaag aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa
     3061 aacaacaatc caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa
     3121 gcgatttttt caactaataa aagagaaatc aaagagatac cctatattcc gctgaacaag
     3181 aaaacgtaca tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca
     3241 ccaatgattc ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac
     3301 aatacttgat gtaatgaaaa gccattattc aacaataaac tgatttcttt attcatcaat
     3361 aagaaaaagg atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga
     3421 taattatata cgtctgtact tgaaataacc gaatacgaat agctattttc aaagtcttca
     3481 aaaatattag tggcataatc agtgtgaaat gcttgattga atcgttccac aaatagtaaa
     3541 atgaaccgtt cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct
     3601 tttattttga tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc
     3661 ttctcgtgag aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt
     3721 atcttgaagt tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa
     3781 gaaagctcag tttctgatga gacattttca aataccatga ttagctgctc atattcttct
     3841 tgcccaataa aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat
     3901 tcatttgtcc gatagagtac ctgtttattt ataataacca aggcaaaacc actcatgata
     3961 ccggtcacta tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct
     4021 tacttaaatg attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa
     4081 atgtataggt catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa
     4141 catcggagaa aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg
     4201 cgaaaaaatc ggtgtttccg ataaaacaat ctctagttgg gaaaattctc gtacaatgcc
     4261 agaccttgaa atgctgtcac ttttacatca gactttaggt ctgccgattg attttccttc
     4321 tattgaatca gctaccgaat caatagaacc aacattgccg gatgaacctc ttgttatcaa
     4381 gaaatcttat tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct
     4441 cgtcttttgt ttggtctatc tttttcttat caattatcaa tataaaaatg aaacggttga
     4501 tcgattcaat ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt
     4561 ggaaaattcc atcgaacatt gggtatgtaa tgatatcttt gttcatggta catggctgtc
     4621 ttttcacgct ggagaggtag aaaaaggtaa gaatcatgct gtaatcaaac acaaaggaaa
     4681 acacgtcatg aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat
     4741 cctcatccaa gactatgacg atgccttaaa aagaagccct caggagccag ataggcatta
     4801 agtaggagat gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc aaaaaaaaac
     4861 aggggtacaa attaattgaa aaagctttga gatgaaagtt cctaaataaa aaagttcaga
     4921 atgataggta aaaaaatgac taactattct agtattaggc tattt
//
LOCUS       DBKOIJ010000170         4931 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000170 DBKOIJ010000000
VERSION     DBKOIJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.174"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            83..970
                     /locus_tag="ACV5NJ_002504"
     CDS             83..970
                     /locus_tag="ACV5NJ_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HHY6486537.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="ACV5NJ_002505"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="ACV5NJ_002505"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HHY6486538.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="ACV5NJ_002506"
     CDS             2272..2718
                     /locus_tag="ACV5NJ_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HHY6486539.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="ACV5NJ_002507"
     CDS             2991..4343
                     /locus_tag="ACV5NJ_002507"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HHY6486540.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="ACV5NJ_002508"
     CDS             4514..4801
                     /locus_tag="ACV5NJ_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HHY6486541.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1395 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt t
//
LOCUS       DBKOIJ010000171         4912 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000171 DBKOIJ010000000
VERSION     DBKOIJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.175"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..412)
                     /locus_tag="ACV5NJ_002509"
     CDS             complement(<1..412)
                     /locus_tag="ACV5NJ_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HHY6486542.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDF"
     gene            572..1078
                     /locus_tag="ACV5NJ_002510"
     CDS             572..1078
                     /locus_tag="ACV5NJ_002510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HHY6486543.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1246..3159
                     /locus_tag="ACV5NJ_002511"
     CDS             1246..3159
                     /locus_tag="ACV5NJ_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHY6486544.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3164..4165
                     /locus_tag="ACV5NJ_002512"
     CDS             3164..4165
                     /locus_tag="ACV5NJ_002512"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HHY6486545.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4367..4573)
                     /locus_tag="ACV5NJ_002513"
     CDS             complement(4367..4573)
                     /locus_tag="ACV5NJ_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HHY6486546.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1816 a          755 c          993 g         1348 t
ORIGIN      
        1 taaaatcgac aagatgacca gaaagcaaga gttcgttgag ctcttgcttt cttttagttt
       61 tagaaaggaa catcctgatt ttatcttcgc taaatccagt caaaagtttt gcagattggt
      121 cgtagataat cggttttttc aataaagaag gggatgtacg aatctcaata tatagttcac
      181 tcaaactgag ttcatctatt ctgtgaacaa gatttttata actaatagaa tgagtagaga
      241 gcatttcggt cacgccgttg tttgtcaatt ttagtaagtg gataaattca tttttggtta
      301 gtggttgacg aattaagttc tgttcgataa acggtatatc atggacgata agccaacgtt
      361 ttgcgcgtct tgaagacgag cagccagaac ctgtatacaa tttaatcatc atacattcct
      421 ccaatagcta agtatttatg aaataataac atgaagaaat agtcgaaaag tgccatttag
      481 aggtgtaaaa taagaaaatg ttgtcacgta taatggaact agctagataa gaaatactat
      541 taaatcaaga aaaaagaaag aaggcacaag tatgtcaaac acagccgacg caatagtagt
      601 gatcgattta caaaaagggg tatgttttga ttcttcgccg atacacgaat tagaaacagt
      661 gacagcatta gtgaataaaa gaatcaaaac atacgaaacg attgggaaac cagttatttt
      721 cgttcaacac gaagatgctc aattagtgcc acaaacaaaa ccatgggagc tattgtcgga
      781 attatctatt cctgagcaca gttattttgt gagaaaaacc catgcgaatt cattttataa
      841 gactaatttg agtgttttat taactgaatt ggactgtcat tcgatcgaat tatgtggggc
      901 acaagcagag tattgtatag atacgactgt gaagtttgct catggattag gataccagct
      961 tatgatgtgt agaggagcaa cgacaactta tgataatcct tttatgaaag caaaggaaac
     1021 aatcgctttt tacgagaaaa tttgggataa acgatttctg acatttttgg agcaataagt
     1081 tgtttcttcg tttaaaaaaa gcagaatgga tactggttta tccattctgc tttattacgt
     1141 aaaatgagaa aataatcctg ttctattttt atgggataaa ccatttattt tttgtataaa
     1201 aaatgtttgc gtttacatat aattctccta gaaacataaa aaggagtgaa tagcttgaga
     1261 acaaaacaag tattaaaggg agcgttggtc atcttatttg ctgtaagtac aagcgtcccg
     1321 caatggacag tatatggaga gagcatttct tccgaatcgg aatctcggga ggtagaaacc
     1381 acagaaacta ctgatgctgg aaatgaaacg aaatcgagta cttcacaaac agaaacaaat
     1441 actgaaacaa cggttgagac atctgttact agcacagatc aagaaacgac cgctaccaca
     1501 gataaccaac aaacagaaga agcaacagct acaaaaatgt caaacgacga atcctcttct
     1561 gctaaagtaa gtgacaaaga gaatgggaca gaaaatgatg atatcggtaa aggtgaaaat
     1621 ttcaatggta aagaatattt gaattacttc ggtaaacaga tgacagaaat acttaatgga
     1681 ccaacagaag acatcaagtc aaaattaata gagttattag tcactgagct tgctgttaat
     1741 gattatggtg tagataaaga gattatcaaa catctgattg ctacttatct attgtacgat
     1801 atcgcttttg caaaaaacag tgtgttgatg tcgatgcttg gaggtacaga tgatgggaca
     1861 acgcctacag aaaacacagg cttctatcca aatacaaaac aacttcatca aactgtacta
     1921 aacaaacaag atggcaaaat gatggatgta tatgcatatt atgtcgatca aggttcagat
     1981 aaaacagtca tgatccatgg cggatttcga gggaattgga ataacgggat cgttacagaa
     2041 gaatacaatg atttttataa ggccgggtat aatttattat ttgttgattc cagagcgact
     2101 ggtaatagcg gaggcgatta tgtgacctat ggacaatatg agtcagatga cgtcctttac
     2161 tggataaatc aagaagttcg agagcggcca tctcaaaaga ttttacttta tggtggttcg
     2221 atgggagcag ctacgatgat gtctgtatta gcgaaagata ttcctgtcaa tgtcaaagga
     2281 attattgaaa attgcggatt tgcatcaatt gatgaacaac tacgttttac gtacagccag
     2341 acagtagccc ccgcactacc ggatgccata aaaaatcagt tagacatcat tggggatcaa
     2401 gagcatgaag atttatttat gggccttttg aaacaatact attttgatca ggagatgcat
     2461 ttggatacaa aagctgctct acctacgatt ggaatgtcag attcattgcc taaattgatt
     2521 atccatggaa cagcagatga tgtagtacct gtgtctaacg cgcaaaaatt atatgagtta
     2581 tcaggagggt ataaagatct tttattagta gaaggagcag ggcatggaaa ggcacaggaa
     2641 gttgaccatg ctgcctacac gaaacatgta acggactttt tgaaagtagt gttccatgat
     2701 cagataaacg taaagtatgt ggatgaaaac aataaaagtc tactcaaaga ccaggacgaa
     2761 atccagcttt acggagctta tggagaaaat tatatgacag agcaaaagac attcgaagga
     2821 tacgaattag caaatgttga aggaccgact gaagggattt ttaatgagac gataccaact
     2881 attattttct attacaaaaa aatcccagta gctccaccaa aaaagcaaga tccgacagag
     2941 aatgcagaca ataagggaaa gaatgaacct cgagaggatc aatcaagtga tgatacgcaa
     3001 ccgattcaga tgacagtgaa agtacaaaaa aagacagatg caaaaaaaag aatgttgccg
     3061 aaaactggag aaaagaaaca ttcgcttctc atttattcag gtggattttt atctgttagc
     3121 acgattatca catggatttg gcgaagaaag agagggtaaa tgaatgaaat ggttcaagcg
     3181 aatagtgatt gcagtaccag ttatcatcgt agttatttta gtcactgtat ttttgattaa
     3241 tcaaatcaca ccaaagccga ttagcttgct tgttagaaaa caatttgaca caagtgaaaa
     3301 agaacaagta tatgcccgac cagatgattt ccagcaaaaa atcgaacaaa tcgatataaa
     3361 aaaagaaata tcctatcctt caaagtacaa aaataataat atggatatct acacaccgaa
     3421 agtggaaaag aaaaaattac cgattctttt ctggatgcat ggaggcgcat atgtaggagg
     3481 ggataaaaat gattgtcgtg attatttgga atatctatgt tcagacacgc agcaaatcat
     3541 cgtaaatata gattatgaac gatcaccgga agcaaaacat cctacaccag ttatccaatt
     3601 gaacgaagcc attcaagcag ctaaaaaaga gataaaagat caagcagatt ggtcgaatat
     3661 ttcgattggt ggaaattcgg caggtgcaca aattagtgga gaatatttgt tagcacttca
     3721 aaatgaagaa attaaaaaag ctgatcatct tgatccgact ttggagaaca agcagattac
     3781 gaagtttatt tctttgtctg gtctgttgga tccttctgca cttactcaag taagtgataa
     3841 aatctctagc tttctttatg aaaaatgtgg atgggcttac tttgataata aggactttga
     3901 acaatcagag aaggtcaaaa gtttagcctt ggtcagacat gcggatcgtt ggacgcaaaa
     3961 cacattctta acggatggaa atacaaatac gtttacgaaa caaatggaag aagtaacttc
     4021 tgtatttgag aaagcggggg tgaaagtcac aaaagtagat tatgcggaga aaaatgctgt
     4081 tttgaaccat gaatatcagt ttgatttcag caataaacaa gcgcaagaaa catatcaaaa
     4141 gttagtaatg tttttcaagg agtaaagtct tatgatgcaa aagaggttgt aatctgtcac
     4201 ttagctccaa gtcacaagga gcaatttcaa taaacaagct tttcatggtt tatgaatatt
     4261 gtaacttgtg actgaggagt tagagatggc aaggggcacc ttttattcga aatttgggaa
     4321 tatcacacga ttattgtcac gatctctttt acgtaaattt tataagtcag atttgttgtt
     4381 ttgatcgttt aaaaaaagca acaaatccga agcctgcgga ggcaagaagg gacaaaaagc
     4441 cgagtggtaa gcaaaagaat gctggttcca ctaatattcc gttgttattg atataaggct
     4501 gggaacaaag atgaatagca aataaaatca atgcactagc gaaaaaaagc aaagaaatat
     4561 ggatagcttt cataagcagc acctcctttt ttataattgt atgataaata gattaagaat
     4621 ctataaatgt gacaagtttg ttaggatgtg aataaaatga tcatctagcg aacttgaaac
     4681 aaagatggct aatgataagc aattaatgat tcagcaatca gataggtaca aagtttacca
     4741 tcatacgtct agaccaataa tagaaaatat gggaatataa tagaaaagat ttaggatacc
     4801 cccaaaagaa tttccccaaa aattcgatgt atcctaaact tttttatttt acataacaat
     4861 catatcaact gtgaaataaa attacaaatg ataacctaaa atgaggccaa aa
//
LOCUS       DBKOIJ010000172         4899 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000172 DBKOIJ010000000
VERSION     DBKOIJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.176"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(125..1735)
                     /locus_tag="ACV5NJ_002514"
     CDS             complement(125..1735)
                     /locus_tag="ACV5NJ_002514"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HHY6486547.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1957..2202)
                     /locus_tag="ACV5NJ_002515"
     CDS             complement(1957..2202)
                     /locus_tag="ACV5NJ_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486548.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2928..4268)
                     /gene="glnA"
                     /locus_tag="ACV5NJ_002516"
     CDS             complement(2928..4268)
                     /gene="glnA"
                     /locus_tag="ACV5NJ_002516"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HHY6486549.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4296..4688)
                     /locus_tag="ACV5NJ_002517"
     CDS             complement(4296..4688)
                     /locus_tag="ACV5NJ_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHY6486550.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1444 a         1053 c          799 g         1603 t
ORIGIN      
        1 aaaaagtctt ttgctttcta tcttttttcc tacaaataat aacaaatcag aaaaagagaa
       61 aagtgacaag aaaattgctt atccccttct cttgctactt ttctctttct atcaagttca
      121 aaaattattt tgtttcgtga tctgctaatg atgctccgac aaatgctttg attaattttt
      181 gcgggcgatt tggacgagaa atcaattctg ggtggaattg gcatcccaca aagaattttt
      241 tctctggcag ctctacgatt tctactaaac ggttatctgg tgagacacct gaaaagacta
      301 gcccttgttc ttcaaacaat tgacgatatt tgttattgaa ctcatagcgg tgacggtgac
      361 gttcttgcac aacatcggct ccatcatatg cttcagccgt cttcgttcct tttttgattt
      421 tacatgggta taatccaagg cgtaaagttc cacccaaatt ctcgatgttt tcctgatcag
      481 ccattaaatc aataatattg tttgctgtat ctggattcgt ttcagctgat gacgcatctt
      541 ctaatccaac gacgttacgt gcaaattcga cacaagccat ctgcatacca agacaaatac
      601 ctaaaaacgg aacgtcattt tcgcgagcat aacgaatcgc ttcgattttc ccttctactc
      661 cgcgatcacc aaatccacca ggaaccaaga ttccatctgc gtctttcaaa cgttcttcta
      721 cattttctct cgttaattct tgtgctttga tccaatcgat ctcgatgtct gaattatagg
      781 cgaaaccaga atgtttcaat gcttcaacta ctgagatata tgcatctggt aactcgacat
      841 atttaccgac aagcgcaatc cgtacttttt tcttcaagtt caagacacgt tcttctaatt
      901 ctttccattc tgtcatgtct gcttctggtg tatctagttt taaatggtcg caaacaattt
      961 gatccattcc ttgtgcttgt aaaactaatg ggatggagta aagtgtatct acatctggcg
     1021 actcgataac tgcttctggt tcgacatcac aaaattgtgc caatttgttt ttcacgcttt
     1081 gagaaacagg ttgctctgta cgtaccacca aaatattcgg ttggatacct aaaccacgta
     1141 gctcttttac actgtgctgc gttggtttag ttttcatttc tccagcagct ttcaagtaag
     1201 gaatcaatgt tgtatggata tacataacat tatctgtacc gacatctgct ttcatttgac
     1261 gaagtgcttc aaggaatggc aaagattcta tatcccctac tgttccccct acttctgtaa
     1321 tgatcacatc tgaatcagtc attttagctg ctcgcatgat tttttctttg atctcatttg
     1381 taatatgagg aataacttga acagttgctc ccaagtattc gcccttacgt tctttacgca
     1441 aaacttcaga atagattttt cctgtcgtca cgttggagta tttgttcaaa ttgatgtcaa
     1501 taaaacgttc gtagtggccc agatccaaat ctgtttcagc accatcatct gttacgaata
     1561 cttctccgtg ctgatatggg ctcatcgtcc ccggatccac gttgatgtat ggatcaaatt
     1621 tttggatagt tacttttaag ccgcggtttt tcaataagcg tcctaaagaa gctgcaacaa
     1681 tcccttttcc gattgatgaa acgacaccgc cggtcacaaa aatatatttt gtcataagtt
     1741 caaaaactcc tttttaattt atgtgcagga aaagtctcag acaataaaca aagctcccta
     1801 ttcttcaata agaataggga gccgaaattt ctgtaaactt ctgaccctct aaaagggtgc
     1861 ccaaggtgta taatacaagc tgactaaggg aaagtcaagt actcaaatga atactatccg
     1921 cttacaattt tttctttttc gatatccttt gagaaattat ttcttaagtc ctttatgagt
     1981 gcaaaaaaaa tttaacccac ataataagct tgatataaac atagctaacg ttaaacctaa
     2041 gcaaatcaga aaatcgttac tgaaaaattt taagtgaaat aatagataaa aaaataaaac
     2101 aaacattatg atagataata tcccacctaa caaagaacta ctttttccta cttgcagcaa
     2161 cagtttatat agcttatcgt agaaacaatc tttttctttc acccttattt acctccttta
     2221 cgtaaaaaac gtagtccaca gttctcggat ttttggcata tcttttctat taaataatat
     2281 caaaaacaaa acaattccac taattttttc aaagatgacc tttttttcaa caaaagtgcc
     2341 ttgtagggat tcattgaagg taacgaatca cataagtaaa aaataaggag atgcgacaaa
     2401 agtcgtgcct ccttatttct aaataaacgg tgttctcctg ccaactcctc ggtcacaagt
     2461 cacaatatcc atcaaacatg agaagctgtt tttgaaatta tttcttgtga ttcggagtta
     2521 aatggtaggc tcacaacctc tggataaacg gtattcaaaa gccagtttct cggtattaag
     2581 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttaa tactcgaaac
     2641 taaacgtctt tttcaaaacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat
     2701 aagttcagcc aattcaaaaa caagaaaagc tgtttttgct tggctattct tattacttgt
     2761 tgcagggcag ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt
     2821 attagctcaa aaaacggcaa aatatgagga actatttttc cgttagtttc tctaatactc
     2881 aggtcaaaac gctgatttca cagcctctaa ttcacggtat tcctgtttta ataaagttct
     2941 agatattgtt ctctttccca ctcggaaaca gtttgtctga atgccgccca ttcgagacgt
     3001 tttgcttcaa caaaatttga gaagatatgt tgtcctaatg cgtcaatcat tacattatcc
     3061 ttacgcaatt ctttgatagc attatggata gttgaaggta agtcctggat atgtgcagct
     3121 tctctttctt cctcatccat tacataaatg ttacggtcaa cagctgctgg cggtgtgatc
     3181 tgattacgga taccgtctag tcctgcttgc aacaatactg ccattgccaa gtatgggttt
     3241 gcagatggat caactgaccg caattccaaa cgtgtggata aaccgcgaga ttctggtaca
     3301 cgtactaatg gcgaacgatt acggccactc catgcgacat aaacaggcgc ttcataacct
     3361 ggaacgagac gtttataaga attgactgtt ggattgcaaa cggctgtgta ggcacgagca
     3421 tgtttcagca aaccacctaa gaaatgataa gctgtttcac ttagttccaa ttcaccgtct
     3481 ttatcataga aaacatttcc ttcatcattg aacaaagaca tattgcaatg cataccagaa
     3541 ccattgatcc caaataacgg tttcggcatg aaagtcgcgt gaagtccatg cttgcgtgca
     3601 atcgttttga caacgagctt gaaggtttgg atattatcac aagcatctac cacatctgca
     3661 tatttaaagt caatttcatg ctgtcctggc gctacttcat gatgtgaagc ttccacttca
     3721 aagcctagac tttctaattc caacacgatg tcacggcggc aattttcacc taaatctgtt
     3781 ggtgcaaaat caaaataacc acctttatca ttcaaggtcg ttgtgatccc accgtcttcg
     3841 tctaatttaa ataagaagaa ttctggttca ggtcctaaat tgaaagaggt aaaaccaagg
     3901 tcgcgcatat ctttcaatgc tcgtttcaaa ttcccgcgag gatcgccggc aaatggtgtc
     3961 ccatccggat tatagatatc acaaattaaa cgagcaactt ttccatgagc actttcccac
     4021 gggaagatca tccaagtgga gagatctgga tacaaataca tatcgctttc ttcaattcga
     4081 acaaatcctt caatcgaaga accgtcaaac atcattttat tttctaatac tttgtcgatc
     4141 tggctcactg gaacttctac atttttgatc gtacctaaga tgtctgtgaa catcaatcgt
     4201 aaaaatcgta cgttttcttc ctcaatgatt cgtttgattt cttcacctgt tatctgtttc
     4261 tttaccatgg tatccctctt ccttttttaa tttattcagt caaagaattg tctttgcaat
     4321 ccttgcgact gataatgacc tgttttgctt aacccacctt gcgccaaaat ctcatcacga
     4381 aaaatttgtc gaacgtcatg atctgtcaac ggcttcttgt tttttgcttc ttgttttttc
     4441 gcctgtgcca tttcgtatac acgtttgatc ccagccatat tcaatccctc tgaaagatag
     4501 tctttaatct caagcaacat atcaatatca ttcagcgaat acatacgtcg gtttccttcg
     4561 cttcgttccg gatgaactaa atcttgttct tcataatagc gaatttgccg agcagacaaa
     4621 tcagtcagct tcataactgt accaattgga aatacggaca tagatcggcg caattccttt
     4681 tctcccatca gacgtcccct ttcgttttat aagttggcta aagtgtacca attaaaaaaa
     4741 atattgtcaa ctgcatatgt taggttttct aacatgagat ctctattttt tataaaataa
     4801 caaaaaaata agaacacaaa aaaggtatct agttgatttt cgtaccagat accttttatt
     4861 ttttattata ctgtactatc ctttattagt gtttttatt
//
LOCUS       DBKOIJ010000173         4896 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000173 DBKOIJ010000000
VERSION     DBKOIJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.177"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            27..845
                     /gene="purR"
                     /locus_tag="ACV5NJ_002518"
     CDS             27..845
                     /gene="purR"
                     /locus_tag="ACV5NJ_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HHY6486551.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1348..2721
                     /gene="glmU"
                     /locus_tag="ACV5NJ_002519"
     CDS             1348..2721
                     /gene="glmU"
                     /locus_tag="ACV5NJ_002519"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HHY6486552.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2815..3786
                     /locus_tag="ACV5NJ_002520"
     CDS             2815..3786
                     /locus_tag="ACV5NJ_002520"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HHY6486553.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3792..3894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3960..4529
                     /gene="thiT"
                     /locus_tag="ACV5NJ_002521"
     CDS             3960..4529
                     /gene="thiT"
                     /locus_tag="ACV5NJ_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HHY6486554.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4610..>4896)
                     /locus_tag="ACV5NJ_002522"
     CDS             complement(4610..>4896)
                     /locus_tag="ACV5NJ_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486555.1"
                     /translation="KSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYR
                     IPIYVIEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1591 a          864 c         1073 g         1368 t
ORIGIN      
        1 aaatttctta aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca
       61 tgacacagta tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac
      121 gttatgaatc tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta
      181 aagaacgagg gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca
      241 tccctgaaat ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat
      301 cagaacaaga ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac
      361 ctgaactgct gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg
      421 atgctgtaat gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc
      481 tgaatgcgcc attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa
      541 gtgtcaacta tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa
      601 gtttgaaacg tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag
      661 tcaacggaat gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat
      721 ttgccgaatc aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg
      781 tcaaagatgt agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag
      841 aataagaggt tgtgaaaaag ctgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
      901 gctttccaca ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt
      961 ttatctagag gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa
     1021 ttgctcctca tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg
     1081 tttaatgaag aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa
     1141 aacagctttt catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca
     1201 ccggttacca aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg
     1261 ggataaaaga aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc
     1321 agcaaactat atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca
     1381 gggaaaggta cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa
     1441 cctatggtag aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg
     1501 atcgttggac acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg
     1561 ttacaagcag aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa
     1621 ggaaaaaaag gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg
     1681 ctgaacaatc tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca
     1741 caagcagaaa acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa
     1801 aaaatcgtgg aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca
     1861 ggaacttact gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac
     1921 aatgctcaag gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat
     1981 acagttgctg cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc
     2041 gcgttagcta aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat
     2101 ggcgtaagct ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca
     2161 gacacattga tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt
     2221 gtcatcgggt cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa
     2281 aactcagtca ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat
     2341 ttacgaccaa aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa
     2401 aatgctcaaa tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg
     2461 ctgggagaag aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac
     2521 aaacatcata cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga
     2581 cctgtcgagg tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt
     2641 ccagaatatg cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa
     2701 aaactccctt atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga
     2761 ctactattta ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca
     2821 aaacattact ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca
     2881 gagaaaattg cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt
     2941 gatggagaaa ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt
     3001 caatcaacga gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca
     3061 ctgaaacgag caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt
     3121 caagaccgta aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt
     3181 caaaaagctg gtgcaacaag aatgctgact ttggaccttc atgcagttca aatccaaggt
     3241 ttctttgata ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta
     3301 gaaaaaggaa tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca
     3361 cgggcacgta aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt
     3421 ccaaaagcaa atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt
     3481 gtactgatcg atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg
     3541 aaagaagctg gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct
     3601 gctttacaaa gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat
     3661 cttccagaag atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga
     3721 gatgcaatca aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat
     3781 aaataataaa taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa
     3841 acccttcgta cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac
     3901 tattttcgaa agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta
     3961 tgggaaaaaa tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt
     4021 cgttgattcc gatccagatc ggcagcagtt tttctatttc attagggcag attccattga
     4081 ctctttttgc gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca
     4141 tcctacattt tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt
     4201 acccgattgc ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag
     4261 gctatgtaga cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg
     4321 gcgcactttc cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg
     4381 cactatgggg gatgaatcct tggctctttt ctttcgtaat gaatggtgcc agcgggttga
     4441 gtacagctat tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt
     4501 ttgtgccaga taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa
     4561 tcatgtccta gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg
     4621 gcagataaga aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga
     4681 ttaaagtagc gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat
     4741 cttcaatcac atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag
     4801 aaaaaatgat cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt
     4861 tttcaacagg tgtttctttt ttgaaaaaag attttt
//
LOCUS       DBKOIJ010000174         4887 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000174 DBKOIJ010000000
VERSION     DBKOIJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.178"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            419..1459
                     /locus_tag="ACV5NJ_002523"
     CDS             419..1459
                     /locus_tag="ACV5NJ_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HHY6486556.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2283..2615
                     /gene="traE"
                     /locus_tag="ACV5NJ_002524"
     CDS             2283..2615
                     /gene="traE"
                     /locus_tag="ACV5NJ_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HHY6486557.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3234..3575
                     /locus_tag="ACV5NJ_002525"
     CDS             3234..3575
                     /locus_tag="ACV5NJ_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HHY6486558.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(3812..>4887)
                     /locus_tag="ACV5NJ_002526"
     CDS             complement(3812..>4887)
                     /locus_tag="ACV5NJ_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HHY6486559.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1693 a          694 c          917 g         1583 t
ORIGIN      
        1 ttagtagtat ataatctatt gttttctagc ctaaagcctt gtcttataag aataatataa
       61 tatatactac tgaaataagg tagtatatat tactagtaat ggtagtatgt tttatatgca
      121 taaggtagtt cttaatttat tttttgtttt gacactggta gtacttaatt atctttaatt
      181 ttaaaactaa attttattca tacgtttttc tggtagtatt taattataaa taatatttat
      241 ttaaagtagt tcttaatgac tttaaaggta gtacataatt cattttattt ctgattttag
      301 gtagtattta attaaaaaac agttttaatg cgtttgcatt ttttatttat ctttgctatt
      361 ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt gaaagtagag gatctacaat
      421 gactgatttt aaatttttta aggcggatag agtttacaac gaattatttt atcaatttcc
      481 aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa gattcaacta agattgccta
      541 tatgctttta aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg
      601 taatgtttat tttacttata cgacaaatga actatgtaga gtattaaact gccaaaaaca
      661 aaaagcgata gcaatcaaaa aagagttgga atcctttggt ttattattac aaaagcagat
      721 gggatttaac aaacagttag ggaaaaataa tcctaataga ctatatctag cagaattaaa
      781 agtctcagaa aatgatatct acttactcga aaaatttgat agagagaata gggaaaacgt
      841 tgataaatca gagggtatga aaatcatacc caccctcgac gaaaaatcag acgctgaatc
      901 ccttggggct caagagggta tgaaaatcat accgtgccaa aacgttgata aatcagaggg
      961 tatgaaaatc ataccagaac ttaataataa tatattagac actaatagac acaatataga
     1021 cactgaaaaa gaccgcctac aagatcaatt gttgttagac aattttgaga caattatgac
     1081 aaacgacagc attgctacgt ttgtccctga acgagtatta aatttgataa aaacattttc
     1141 ttcaagttac agtgaagctc aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc
     1201 tgaaatagaa agtgatattt cgatagtttt tgaagaactc gatagttatt atgtcaatgc
     1261 agaacaagaa ttatacacga cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt
     1321 cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat
     1381 agctgctaaa gtatcaagtg aaaaacgttt gaattatgca agctccccaa gcactattac
     1441 gaaagactgg ttagagtgaa actttactga caaagtgatt gttgcttcat ggagtaacat
     1501 taaaattaaa aattttttat tattctaatt atattacgaa tattacaaaa taatagctat
     1561 tttaatagaa tttataagtt aaaatattat gataaaaatc acaaacgtag ccttttacgt
     1621 cataagtgtt aaaagaaata attaatttca aatatagaaa agggaacgta tgtttgcata
     1681 ctcctttttt ttgagttata ataatacgcg aagagagata atttcattat ttaaacaagc
     1741 taaaaatctt gatattactt cttttcaggc aatacgaata ttattcgctt acattttttg
     1801 tataaattta attgtctgct atgcttgtga tgaattgatt ttcgttttgc acttttggat
     1861 gataaatttg gacaattgaa aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg
     1921 gtaaccttcg tcagcgttca tacacggcat taagatttgg cccttaatgc gttaaccttt
     1981 cagtgttacg gtattacccg caatggattt agtataacag atattcaaaa gtgataccac
     2041 tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta tgcctaagca
     2101 taatgttata aaaatgtccg tgtggctctt tgccgtatga cgaacgctga aattagaggg
     2161 aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat caattctcta
     2221 tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagaaattgg gtggtttatt
     2281 ttatgtcata tacaggaatt ttgagtctca aagatatttg tcactacggt aaacggtgta
     2341 ccgctacaga gaaaatcacg aagaaactat caactggcca aagtaaaacc gttgtgcagt
     2401 gtaagaaata tataatccag aaagacaaag tctcagagga aatgatttat tacattggga
     2461 aacaaaagca aataatttta aaagatccta tccccttaaa agagctatat ccaacaatca
     2521 aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt
     2581 gtaccgcaaa agggatgggg cgtttgatcg gctgaattga tcattctcat aacttgtatt
     2641 ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaggaact tagagtcact
     2701 agaaaaataa ggagtagact tgatctatca acaattccct tcggggagtg acggggtagg
     2761 ttttaacgat tggtaacgta aaaaattggt caatcgagta aatgcgcttg tttggatagt
     2821 ggtgaaatga atttgtgtaa ataataagcg cagagtaaac aagagggaat ttagaagtta
     2881 gacaagttag aatagcgtct agattaatca tgagcagcta ctaagggatc tagactttga
     2941 cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca caagaaattg
     3001 tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tgattattgg atcaatcgag
     3061 catttaccgc tgtctactag ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt
     3121 tatgtatttt taagtaaacg aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa
     3181 aaattaatat aaagcataga gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa
     3241 attatgatcc gaatatacga ttgggcacac atactattaa ggtttctttc caacgttggg
     3301 attacaaagg atttgtgacg tttagaaggg gcggaaattg taaaggatta gatgttttag
     3361 cattagacga ggatgattta tacgatcaaa cattgactga taatccaatt ggattcggct
     3421 tgctacctga agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg aatgataatg
     3481 gagacgaatt atctgttgaa gatacttggt catatttgag tgattatatt gtcagctttg
     3541 aaatcattga atttgtagcg gataaagaag aataaaaact ggaggaataa attttacaga
     3601 taaaggctta gaaggagtca cgacgattaa taaaaaggta gtattacctt aggcattgag
     3661 taaggaattt atttagtcta atggtgataa gtcaagatga aatttaagat tttctttatt
     3721 gagtaatatg ttttgaagcg cacgaaacta gacccgatca aaatcaaatt tttttcgggt
     3781 tgattcaagg atttgcgtca ataacggatt gttatttctc tatcacactc cttggtggcg
     3841 tatagagttg gtgtttacgt agtagcgtat ccaccagacg cgtaaatttt cttgcggtta
     3901 ggacgagtgc acgtttgtgt tgatgctttg gaacctcctt atatttactt tgataaaaag
     3961 ctttgtattc aggaagataa ttttttacag agttggcagc ttcaactaag tagtatctaa
     4021 aatatcgatt tccttgtttt gtcagaggtg tattttgcga ctgatagttt ccagattggt
     4081 tcactttcca acttaatcca gcgtattttg ccaattttgt ctgatcttca aatctttcga
     4141 tttgaccaat ttcagcgatt aaaccggcag catagacttt accaacgcct ggaatactgg
     4201 ttaagcattg atattctgga atgacaacaa ttacttgttc gatggcttta tctaattctt
     4261 tgatattttt ttcaagtgct cgtatttctc gtacgagaac acttaaaaca acattaattg
     4321 attcttgtgc aagagcacct aggcggtaac tcatggtaat tgcacgttga attgctttag
     4381 cgattttttc tggttgcttg aaacggcctt ttcctttttc ttgaagtaaa tcacagaaag
     4441 cttctaaagg aagctcagca agttcatcca atgtgaaatc ttccgtcatg agtgaaatga
     4501 gggttgcact aaaaagactc gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt
     4561 tgtacgttag attttcaaga aaatgttgct ttgtacgaat caattgctta ataagttgat
     4621 agcgagtacg tgttaaacgc tgtaaggcca tgtatttctc ttctttgata ggggaagttg
     4681 tgaatcgttg aatacgcaag aaatcagcga ttcttaatgc atcgtttcga tcagttttat
     4741 tctcatcaaa catgcgacta aattgtttca cccgaaaagg attttcaatc gttacaagtg
     4801 tgttgagctt ctttagtttc tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca
     4861 tggacgttga ctccatgcca atcacaa
//
LOCUS       DBKOIJ010000175         4757 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000175 DBKOIJ010000000
VERSION     DBKOIJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.179"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACV5NJ_002527"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACV5NJ_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HHY6486560.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACV5NJ_002528"
     CDS             724..1878
                     /locus_tag="ACV5NJ_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HHY6486561.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACV5NJ_002529"
     CDS             2093..3061
                     /locus_tag="ACV5NJ_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HHY6486562.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACV5NJ_002530"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACV5NJ_002530"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HHY6486563.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACV5NJ_002531"
     CDS             4091..4699
                     /locus_tag="ACV5NJ_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HHY6486564.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DBKOIJ010000176         4750 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000176 DBKOIJ010000000
VERSION     DBKOIJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.180"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(136..1098)
                     /locus_tag="ACV5NJ_002532"
     CDS             complement(136..1098)
                     /locus_tag="ACV5NJ_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6486565.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACV5NJ_002533"
     CDS             complement(1100..2539)
                     /locus_tag="ACV5NJ_002533"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HHY6486566.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACV5NJ_002534"
     CDS             2724..4670
                     /locus_tag="ACV5NJ_002534"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HHY6486567.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1511 a          813 c          779 g         1647 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt
//
LOCUS       DBKOIJ010000177         4749 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000177 DBKOIJ010000000
VERSION     DBKOIJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.181"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(144..506)
                     /locus_tag="ACV5NJ_002535"
     CDS             complement(144..506)
                     /locus_tag="ACV5NJ_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486568.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(580..960)
                     /locus_tag="ACV5NJ_002536"
     CDS             complement(580..960)
                     /locus_tag="ACV5NJ_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HHY6486569.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1042..2217
                     /locus_tag="ACV5NJ_002537"
     CDS             1042..2217
                     /locus_tag="ACV5NJ_002537"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HHY6486570.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2294..2878)
                     /locus_tag="ACV5NJ_002538"
     CDS             complement(2294..2878)
                     /locus_tag="ACV5NJ_002538"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HHY6486571.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3079..4077
                     /locus_tag="ACV5NJ_002539"
     CDS             3079..4077
                     /locus_tag="ACV5NJ_002539"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHY6486572.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4189..4698
                     /locus_tag="ACV5NJ_002540"
     CDS             4189..4698
                     /locus_tag="ACV5NJ_002540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HHY6486573.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1508 a          895 c          836 g         1510 t
ORIGIN      
        1 tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc tttagcagaa
       61 catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa aattcaaact
      121 tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc gtaagttcca
      181 agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa ttcttctctt
      241 tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt aatatgtgta
      301 tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc ttttaagaaa
      361 ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc aaattccaac
      421 ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg caacataatt
      481 gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt ctatggctta
      541 cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat tttctttccg
      601 ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa tagtggtaaa
      661 tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa cagctggatt
      721 tgttttttct aacgttctta aagataaact gatttttttg gaatattcat caatgtcgat
      781 gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt tcgtataacc
      841 ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag aaacgaatgc
      901 gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt tataagtcat
      961 ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa agaagcgacg
     1021 aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag aaaagataca
     1081 gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt acttcctttg
     1141 tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt tagtgaatac
     1201 atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca ggctatcatt
     1261 cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt ttttacaagt
     1321 ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg cgattctatt
     1381 ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa tcaacgtcac
     1441 atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt gtctgacatg
     1501 gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc tcacaatcct
     1561 ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg tttaaaacat
     1621 caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga tcatacattt
     1681 acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt tacttccgca
     1741 accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa aaatccagag
     1801 atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat caacactttt
     1861 ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag ccaactgatt
     1921 ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta tcttccgaaa
     1981 gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac cgcctatatt
     2041 tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt attaaatcct
     2101 ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc ttgtccagaa
     2161 gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat cgattaaatc
     2221 atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca taattactcc
     2281 gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca tgtacaggtc
     2341 ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc ccttctagta
     2401 catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct ttatacgctt
     2461 caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta gctggcacat
     2521 ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa tttgccatag
     2581 ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa ctttcgccat
     2641 aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc atgaaatcag
     2701 gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt tcaatgaaat
     2761 tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca ccacggtttg
     2821 ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg aatgccatat
     2881 tgtttcctcc taatatttgc atacttcata ataataataa cataatacct aacctctcgt
     2941 aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta taacttaccg
     3001 tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct gctatagtgt
     3061 gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc aggtcctgta
     3121 gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat cattgacagt
     3181 ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat ttacgatatt
     3241 cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa acaattaact
     3301 acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg tgaagacgaa
     3361 gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat tttagcttta
     3421 ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc atttgaaaac
     3481 cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt
     3541 gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat tgcaagcgaa
     3601 gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt atcacgcttg
     3661 aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc aggcaataat
     3721 ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg
     3781 attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct ttcttcttgg
     3841 ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac ttctattcca
     3901 ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact gattgctact
     3961 gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa tccaaaggaa
     4021 cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc tagttgattt
     4081 tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt
     4141 tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa
     4201 actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa
     4261 gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt gactttggcg
     4321 gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa cgccattatc
     4381 actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc gttgcaacat
     4441 attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt gtcaatcgtg
     4501 aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt gaaacctggt
     4561 attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt agatgatatc
     4621 gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc agaaaaaaga
     4681 agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta gacgagttct
     4741 ttctttttt
//
LOCUS       DBKOIJ010000178         4676 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000178 DBKOIJ010000000
VERSION     DBKOIJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.182"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(253..1326)
                     /locus_tag="ACV5NJ_002541"
     CDS             complement(253..1326)
                     /locus_tag="ACV5NJ_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6486574.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(1323..2150)
                     /locus_tag="ACV5NJ_002542"
     CDS             complement(1323..2150)
                     /locus_tag="ACV5NJ_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6486575.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(2151..2957)
                     /locus_tag="ACV5NJ_002543"
     CDS             complement(2151..2957)
                     /locus_tag="ACV5NJ_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6486576.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(2969..4054)
                     /locus_tag="ACV5NJ_002544"
     CDS             complement(2969..4054)
                     /locus_tag="ACV5NJ_002544"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486577.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(4071..4613)
                     /locus_tag="ACV5NJ_002545"
     CDS             complement(4071..4613)
                     /locus_tag="ACV5NJ_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486578.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         1424 a         1015 c          764 g         1473 t
ORIGIN      
        1 gcatagtttc caattacttt atccttctgt aactttttca atcttagatt tactagcccg
       61 tctcgggccc tattttacaa tggttttgtc cacttttctc tggtagaaaa acgtggtaga
      121 aaaaataaaa taacacaaaa aaagcggcgt ttcatccgct tttggtagaa gattggaaaa
      181 ctcagataga aaaaggacga tttttagctc tttagaacgt tgataagtcc ccattcttac
      241 tgagttttgc cattatctcc tatacatctt gaattctaag aataggtcat tatagatgcc
      301 taggtatttc gatcctagat ctttgtagac ttcaagatgg gagatcgtgt catcagatgg
      361 atagaattgt tcgtccgacg aaatgtcttt tggcaataac gcttttgctt cttcatttgg
      421 tgtagaataa ccaatatact ctgcattttg tgccgcattt tccggttcta gcataaagtt
      481 gatgaaatca taggcacctt ctttattttt cacggttttt ggcataacga tattatcaaa
      541 ccaaagattg gatccttctg gtgggatcac ataatgtaaa tgttcatttt cccacatcat
      601 atcggcagct tcgccagaaa atgtcactgc cacggaggct tcttcattga tcatatacat
      661 tttgatctca tctgccacga ttgctttgac atttggtgtt aaatgattca atttatttgc
      721 tgcttctgtc agctgtgcca tattcttact attcaaagaa tagcctaaac tattcaaaga
      781 aagccccata acttctcgtg caccgtcaat cagcatcaaa ctgtctttta attccggctt
      841 ccacaaatca tcccaatgct taatttgatt tgcatcaaag aatttatcgt tatatacgat
      901 ccctaaagtt ccccaaaaat atggaatcga atactgatta tctggatcaa aatccaagtc
      961 taaaaatcgt ggatcaatat tatttaatcc tttgatcttc gaatgatcta atggaagtac
     1021 tagtttttct ttgatcattt tttggatcat atattcactt ggtatagcga tgtcgtagtt
     1081 tgtcccacct tgctggattt ttgtgaacat cgcttcatta gaatcaaatg tttcataatt
     1141 cactttgtat ccatattctt tttcaaattt cgtgatcaaa ctaggatcga tataatctcc
     1201 ccagttatag atggtaagta cctttgctcc actcatgcca cttgtttttt caagttgacg
     1261 aacaccaagc agcagcaccg caataatgat gagaatacct aaaaacagag attgtaattt
     1321 tttcatcgca gccgtgccac cccctcgcgt actcttctgt ttttcttcag ccgttttgga
     1381 cggttttctt tactaataaa gtagtatcca atgaccaata tcatggagaa caagaaaact
     1441 aacgcactta gcgcattgat ttctaaactg attccttgtc ttgcacgaga atagatctct
     1501 actgagagcg tcgtaaagcc atttcctgtg acgaaaaatg taaccgcaaa atcatcaaga
     1561 gaataggtaa atgccataaa atatccagct atgattcctg gagaaagata aggcaaaatg
     1621 atatttttga ctacttggaa attattagca ccaaggtctc tagccgcatc gaccattgaa
     1681 tcgttcattt cctgcaattt tggcaagacc atcaagacga cgattggaat gctgaaagca
     1741 atatgggaaa gcaatacact ctgaaaacct aatccgaatc ctagtagtgt aaagaaaatc
     1801 aagaaactag caccgataat aacatcagga gagactaata agatgttatt catgctcaac
     1861 aacgctgtcc gtgcacgtcg ttttttcgtg taatagattc ccatagcccc aaatgtcccg
     1921 atgatcgtgg cgatcaatgc agataagaaa gccagcatga acgtattcaa tacgataatg
     1981 atcaaacgtg tatcttcaaa aacagcttca taattttccc aagtaaatcc tgtaaagctg
     2041 ttcatcgttc ctccttcatt gaaggaataa aaaatcagat agaagatcgg tgcatataag
     2101 aataaaaaga cgatccctaa ataaagatac gaccatttaa attttttcat ttatgagcga
     2161 cctccttttt tcttctcgcc tgtcaggagc atcacgatga acatagcaat gattaaaatc
     2221 acaccgattg tagaacccat cccccagttt tgtgtaacca agaaatgctg ttcgatagcc
     2281 gttcctaaag tgatcacccg atttccgcca atcagacgag tcagcatgaa taaagaaaga
     2341 gaaggaataa atactgcttg gacaccactt ttcacaccat ttaatgataa cggaaaaatc
     2401 actcgtcgga aggtctcaaa gctattcgcg cctaaatctc tactagcact gatcaaagaa
     2461 ggattcatct cttccaatgc attgaaaatt ggcataatca taaatggaat ctcgatatac
     2521 gttgccacga acagaaaact gaagtctgtg aataatatct gtttcggccc gatcccgata
     2581 aaatcaaaga aatgattcac gctgccgtgc acactgaaaa tcccaataaa tgcgtaagct
     2641 ttaagcagca gattcaccca tgtaggcaaa ataatcagca tcagccataa ttgcttgtgc
     2701 ttcagttttg ttaaaaaata agcagtcggg taactgatga tcagcgtgaa aaacgtgatc
     2761 aaaaatgcat accatacaga attgatcgtc atagaaagcg atgtccccga cgtaaaataa
     2821 gatagataat tattcagcgt gaattgccca ttcatatcaa aaaaagattg ataaatgatc
     2881 attaatacag gcgcgatcac gaataaaccg agccacaaaa tataagggac ggaatagatc
     2941 ctgcgcattg ttttcattct tgtcccccct attcttcgta gctgtcaaga cgtgcatcga
     3001 attcttcttc agactcgttg aaacgcatga catggatatc ttctggctca aaggccaatc
     3061 ctactttgct tccttcttta gcttttttcg ttgaatggac catccattca ttttgctgtt
     3121 catcataaca gatgatttca taatggacac cacgaaacag ctgagtatcg accgttacga
     3181 ctagttttcc tttatctggt gtcgtgatcg tcaaatcctc tgggcgtaaa acaacttcaa
     3241 ctggttcatt tagacgcatc ccgccatcga cacattcaaa ttgcttacca acaaactcaa
     3301 ctagattatc ttcgatcatt acaccgtcca cgatattgct ctccccaaca aaatcagcaa
     3361 cgaaatgatt gattggctca tcataaatat ctacgggtgt accactttgg acgattttcc
     3421 ctttattcat gacaaaaatc tcatcactca ttgccaacgc ttcttcttgg tcatgagtaa
     3481 caaaaataaa cgtgattcct aatcgttgtt gcagatcccg cagttcatac tgcatatctg
     3541 tacgtagttt caaatcaagc gccgataacg gttcatctaa tagcaatact ttcggttcat
     3601 ttacaattgc tcgtgcaatc gctacacgct gtctttgacc accagacatt tcactaatct
     3661 cccgtttttc gtatccagga agctggacca tacgcaaagc atcttttacc ttcttttcga
     3721 tatctgattt ggacattttc ttgatcttca atccaaaagc aacgttatca aaaacattca
     3781 tatgaggaaa aagtgcataa tcttggaaga ctgtatttac ttgtcgctta ttagctggca
     3841 aatcattcaa ccgtttccct tcaaaataga tgtccccttc tgtagcttcg gtgaatccag
     3901 ctataatacg taggatcgtt gttttaccac agccggaagg accaagcagt gtataaaatt
     3961 ttccttgttc gatctcaaaa cttacttttt ttaatactgt ttcatcatca tagcgtttca
     4021 ctacttgatc aaatgaaatg attggtttcc ccacgttttt accccctata ttacaaatat
     4081 gattgcgttg caacaatcaa tacttttgtt cgttttttga gatgattcct taattgatgt
     4141 tgttttgtcg cttgataata gatcgactgt cctgcttttg ccacatagac cgtctctcct
     4201 aagcttaact ctaattcccc ttctaataca aaaataaacg tttcagataa tgaaggttca
     4261 aacgtcttat attctcctgc accttcaaaa gtcagcaaga caggctccat ttctttttca
     4321 ttcgaatcag ccacgagcca ttccaattca tacccgtttt cttcatcgaa ataaaccgta
     4381 tgatcttctt ttgaataaac cacttgcaga ttcgctgctt cttgatgaaa aaattcttct
     4441 ggagtcacac ctaagacttc aagaatagaa aagaatgttt ccattgatgg tgaacttaaa
     4501 tctcgttcca actgggaaat ataccctttt gacaaatctg tgcgttcgcc tagttcttct
     4561 tgtgtcaaat tcttttggat gcgcaaattt cttaacttct caccaatttc cataatttcc
     4621 tccgaatcga agttttctaa tagtaaactt cctgtttact accactcgta ttagta
//
LOCUS       DBKOIJ010000179         4667 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000179 DBKOIJ010000000
VERSION     DBKOIJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.183"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(104..1336)
                     /locus_tag="ACV5NJ_002546"
     CDS             complement(104..1336)
                     /locus_tag="ACV5NJ_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HHY6486579.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACV5NJ_002547"
     CDS             1591..2160
                     /locus_tag="ACV5NJ_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6486580.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACV5NJ_002548"
     CDS             complement(2338..2778)
                     /locus_tag="ACV5NJ_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HHY6486581.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACV5NJ_002549"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACV5NJ_002549"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HHY6486582.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACV5NJ_002550"
     CDS             3732..4655
                     /locus_tag="ACV5NJ_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HHY6486583.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1412 a          866 c          925 g         1464 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttc
//
LOCUS       DBKOIJ010000180         4640 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000180 DBKOIJ010000000
VERSION     DBKOIJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.184"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..727
                     /locus_tag="ACV5NJ_002551"
     CDS             <1..727
                     /locus_tag="ACV5NJ_002551"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0070085 - glycosylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HHY6486584.1"
                     /translation="IDFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYG
                     LPVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIP
                     VGYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNT
                     EIFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLY
                     QYIGKKVKRPISADSVLLEEDVE"
     gene            724..1872
                     /gene="neuC"
                     /locus_tag="ACV5NJ_002552"
     CDS             724..1872
                     /gene="neuC"
                     /locus_tag="ACV5NJ_002552"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HHY6486585.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1845..2522
                     /locus_tag="ACV5NJ_002553"
     CDS             1845..2522
                     /locus_tag="ACV5NJ_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HHY6486586.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2473..3654
                     /locus_tag="ACV5NJ_002554"
     CDS             2473..3654
                     /locus_tag="ACV5NJ_002554"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HHY6486587.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            3667..>4640
                     /locus_tag="ACV5NJ_002555"
     CDS             3667..>4640
                     /locus_tag="ACV5NJ_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHY6486588.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEV"
BASE COUNT         1690 a          594 c          907 g         1449 t
ORIGIN      
        1 aattgatttt ttgtctactc cttttgatga agaaagtgca gactttctaa aacaaattgg
       61 tgtacaagga tttaaaattg gctcaggaga tttaacaaat ttaccttttc ttagaaaaat
      121 agatatgtat gggttgccag ttttattatc tacaggtatg gctgatattc atgaggtcag
      181 tgaggctgta gattgtttca tagatagtcc agttactatt ttacattgta cttcaaatta
      241 tccggctagt tctgaagata ttaatttatt ggcgattaac acattaaaag aaatgttcgg
      301 gatacccgtt gggtactccg atcattcatt gggatatgat gttggtattt gtgctgttgc
      361 gatgggtgct aaggtaattg agaaacattt taccttggat aaggaactac ctggtccaga
      421 tcataaagct tctttagatc ctcaggaatt tgcagatttt gtaaagcata ttagaaacac
      481 tgaaatattt ttaggtgatg gtgttaagcg agcaatgcct tctgaactta gtacaaaaga
      541 agtatcaaga aaaagcctag tggtcactga gaatttaaat gtgggagata ttcttacaga
      601 gaaatctctt acaataaaac gtcctggaaa tggaatagct cctaagtttt tatatcaata
      661 tattggcaaa aaagttaaaa gaccaataag tgcagatagt gttctgcttg aggaagatgt
      721 tgaatgaaaa aaatacttat agtaagtgga acacgagctg attatggaat atataaacct
      781 gttgctgaga atttactaca agataattgg gatgtatcat ttgttgtttc tggaatgcat
      841 ttatcccata aatatggatt tactaaagaa ttgatcttag ctgacggatt tagaattagt
      901 ggagaagttc aaagtttgtt tttagagaac aataagggaa acatggctcg gtctgtggca
      961 ttagagataa atggtttcac aaatatattt gaacaaacta gccctgattt tgtattatta
     1021 ctaggggata gaggagaaat gttggcagct gcgatagctt gtttgtatct tggaatcaaa
     1081 acagcacata ttcacggagg cgaagtgtct ggtactgtag atgaatcaat aagacatgcc
     1141 ataacgaaaa tagcatctgt tcattttgct gctacggaaa aaagcaaaga gcgtttaata
     1201 aaaatggggg aagattcttg gagagttttt cccgtcggag ctcctagaat agatactatt
     1261 ttaaataata gattacctag ttttagagat ataaaagaaa aatatcatct aaaatttgaa
     1321 aagaaaaaat acgttttgca agtatttcat ccagttgtaa ccgaactaga aaatatagaa
     1381 aaacaaattt tagctttgat caattcaatg gaacaacaat ctcaaccaat tatttgcatt
     1441 ttacctaata gtgatgctgg tagcgaaatt atcagaaaaa tgtaccagaa atatttaaaa
     1501 acggatatta tttatattac gaatttagag ccggaggaat acctgactat attaaaagag
     1561 tgcaaattta tgatcggaaa ttcttcttct gggataattg aagctgcatc attcaaaata
     1621 ccagtaataa atttaggaac cagacaaaat ggacgagaac aatctaaaaa tattataaat
     1681 gcaaatctaa atgaaaatga aattgaaact gctttgaata aaatacaaga tcctcagttt
     1741 ataaaaagct tgaaaaatgt taagaacatc tacggtgacg gcaacagttc tagaagaata
     1801 acaaaaacat tgaatcaaat attgaatgat ggggaagata ttaagtggat tcaaaaacaa
     1861 atagcttatt aaaatatact agtgatccat acattattat tggggctggc ggacacgcaa
     1921 aagtcattat tgaaacctta agattagaaa ataagttaat atatggattt tttgatgata
     1981 agcaaaataa aatgcaagat ctgaaatatc ttgggaaaat aaacgaattg aatagctggg
     2041 cggatagcaa aaaaaaaatc tataaattta ttgtagctat tggtgactgt gtagtaagaa
     2101 aaaagattat aaaaaaattg aatatgccaa gtgagcaata tggaatagct gttcatccaa
     2161 gtgctatagt ggcaaaagaa atttcaattg gttatggaac tgttattttt gcgaatgctg
     2221 taattaaccc agatgctgta attggagagc atgctataat aaacacaggt agcattgttg
     2281 agcatgattg tagaattaat aactatgttc atttatctcc aggtgtatgc ttagcgggtg
     2341 gagtacatgt aggtgtagga actcaaatag gaattggttc tcaatgtatc cagatgaaga
     2401 aaattggatc ttggtgtatg ataggcgcag ggagcactat tgttaaggat attccctcac
     2461 attccttagc ttatggcaat ccagctaaaa ttaagaaaga aggaattaat tttgaattct
     2521 agaattttat tatcaagtcc tcatatggga ggaacagaag aaaaatatgt tgctaaggct
     2581 tttaaagata attggatagc gccattaggt gaaaatgtgg ataaatttga agaaagtatt
     2641 acaaaatata ctggttctaa gggcggagct tctgcgttaa gttcaggtac agctgccata
     2701 catcttgcct taagactgct taatgttgga aaaaacgata ttgtctttgc tccttcattt
     2761 acttttatag ctacagtaaa tcctatttta tatcaagggg ctgtaccagt gttcattgac
     2821 tcagaaccta atacatggaa tatgagccct gttgcactag agagcgcatt agaagaagct
     2881 gaaagaaaca accaattacc taaggcaatc atcgtagtga atttatatgg tcaaatggct
     2941 gattatgaac gaattttagc tattgcaaac caatttaatg ttccagtgat agaagatgca
     3001 gcggaatcgt taggttctac ttataaggga atttcgtcag gcttatttgg gacaattggg
     3061 atctattcat ttaacggtaa taagatcatc acaacctcag gtggaggagc tctagtgtct
     3121 agtgataaaa aatatgttga gcattcaaga tttttagcaa cacaagcaag agatcaagca
     3181 ccgtattatc aacatagcga aataggttat aattaccgtc taagcaatat ttctgccggt
     3241 atcggacgtg gacaaatgga agtattgaat gatagagtag ctaaacgaag agaaattttc
     3301 aatcgatata aggaagcttt ttctggaatt gatagaattg aaatgatgcc agaagtggat
     3361 ggcatggtgc caaatcgttg gttgtctact ttaacgatca atgattataa ttctattgat
     3421 caagtagtta acgtaataaa taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg
     3481 aaaccactac atttgcaacc agtatttcaa ggggtaaaaa tgtattacca tgataataat
     3541 cttagtgtgt cagaaaagtt atttaatagc ggcttatgct taccttctgg aagtaatatg
     3601 acagatagtg aacaagaatt tgttattgat gagtttaaaa aaatattcaa gtaggtgtga
     3661 tattttatga acataggaat tattggttta ggttccatgg ggaaaagaag acttagactc
     3721 attcaagaac actttaatga tattttacta tgtggtgttg atactaatga ggcgagacga
     3781 aaggaagttg aaaaggaatt tggaatacca acttataata gtctaacaga ggcaaaaaat
     3841 ttgtttgaaa taaattcagt ttttgtttgt acatccccta taacccatga atttattata
     3901 aaagaggctt taaatttaga ttgtaatatc tttacggaaa tcaatttatt aaatgaatat
     3961 tatgatgaaa taatcgaagt agctaaaaag aaagatagat tactttatct atcttctaca
     4021 tttttgaaga gaaaagaaat tcaatatatt aagtctaaag tgacgcataa taaaaaaatt
     4081 tcttatcaat accatgtagg acagtattta cccgactggc atccttggga agattatcgc
     4141 aatttttttg tttcaaacaa aaaaactaat gggtgtagag aaatattcgc tattgaatta
     4201 ccttggatta tagatacatt tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta
     4261 tccaatcttg atattgatta ttcggacagt ttcactctta ttattgagca tgaaactggt
     4321 atagttggaa ctttaagtgt agatgttgtt tcaagagtag ctaaaagaaa tttaactata
     4381 attggtgaaa agattttaat agaatgggaa ggtaacccgg attctctatt ttcatggaat
     4441 gaagaaactt ctagtatgac agatattcaa ttgtacgatg aggttatgca tgataataat
     4501 tattcacata ctattattga agatgcatat ttagaggaga ttaaagaatt tttcacgctt
     4561 ttggaaaaga aaatggaacc ccaatatact tttgaaaaag ataagtatat tattaactta
     4621 attaacgaca ttgaggtaaa
//
LOCUS       DBKOIJ010000181         4603 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000181 DBKOIJ010000000
VERSION     DBKOIJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.185"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..1128)
                     /locus_tag="ACV5NJ_002556"
     CDS             complement(<1..1128)
                     /locus_tag="ACV5NJ_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HHY6486589.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACV5NJ_002557"
     CDS             complement(1164..1874)
                     /locus_tag="ACV5NJ_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6486590.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="ACV5NJ_002558"
     CDS             complement(1942..2757)
                     /locus_tag="ACV5NJ_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HHY6486591.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACV5NJ_002559"
     CDS             complement(2768..3736)
                     /locus_tag="ACV5NJ_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HHY6486592.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
BASE COUNT         1429 a          950 c          685 g         1539 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgc
//
LOCUS       DBKOIJ010000182         4570 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000182 DBKOIJ010000000
VERSION     DBKOIJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.186"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..300)
                     /locus_tag="ACV5NJ_002560"
     CDS             complement(<1..300)
                     /locus_tag="ACV5NJ_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HHY6486593.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACV5NJ_002561"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACV5NJ_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACV5NJ_002562"
     CDS             complement(1014..1304)
                     /locus_tag="ACV5NJ_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486594.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1646..1942
                     /locus_tag="ACV5NJ_002563"
     CDS             1646..1942
                     /locus_tag="ACV5NJ_002563"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486595.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACV5NJ_002564"
     CDS             1949..3901
                     /locus_tag="ACV5NJ_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6486596.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACV5NJ_002565"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACV5NJ_002565"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HHY6486597.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1562 a          803 c          755 g         1450 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt
//
LOCUS       DBKOIJ010000183         4482 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000183 DBKOIJ010000000
VERSION     DBKOIJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.187"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            105..1439
                     /locus_tag="ACV5NJ_002566"
     CDS             105..1439
                     /locus_tag="ACV5NJ_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HHY6486598.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1480..2391
                     /locus_tag="ACV5NJ_002567"
     CDS             1480..2391
                     /locus_tag="ACV5NJ_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6486599.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2453..3241
                     /locus_tag="ACV5NJ_002568"
     CDS             2453..3241
                     /locus_tag="ACV5NJ_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HHY6486600.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            3238..3854
                     /locus_tag="ACV5NJ_002569"
                     /pseudo
     CDS             3238..3854
                     /locus_tag="ACV5NJ_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            3856..4374
                     /locus_tag="ACV5NJ_002570"
     CDS             3856..4374
                     /locus_tag="ACV5NJ_002570"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HHY6486601.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1345 a          763 c          903 g         1471 t
ORIGIN      
        1 aataattttt ttagaatcat accttataaa ttgttatttt cagaacaaaa tgatatcgtt
       61 atattaaaac cggcatcggt tacattaaaa tttggaggta aaatatgaaa aaattattgt
      121 ttacagggtt gctggcaagt tcagcattgt ttttattagg aggatgcgga tcaagtaatg
      181 caagcgatgc cagttcaagt gcagcaaatt cttctgaaaa aacaagtgat ggaagaaaaa
      241 atattgattt ctggtccttt tggggatccg gtgcacgaaa agaagctatc gaagaaatca
      301 ttgaggattt caatcaatca caaaacgaga tccatgtaaa ttataagtat cagccatggg
      361 gtgacatctg gactaaatct ttatctgcaa taacagctgg aaatgcaccc gatgttattg
      421 tacaggacat aaattccgtt gcccaaagag cagaagcaca gcaagcaact aatctctcca
      481 aatatgtaga aaaagacact tctaaggatt tttatccgca gctttgggat acagtagaat
      541 atgaaggtga tccttatgcg atcccattca atacagatac acaagtcatt ttttataata
      601 aaaagatatt taaagaagct ggaatcagtg aacaagatct accgacaact tgggatgaat
      661 tagaagaaac agcacgtaaa ctagataaaa aagaaggaaa agaatttaca cgcatcggtt
      721 tttatccatt atggaattta ggggctgatg tatgggcgct aaacgcagat aacgggacta
      781 gttggtttga caaagatgaa aaagtcaaga ttgatactaa aaataaagtg gaagccttgg
      841 agtggatcca agactggcaa gattattatg ggaaaaatac gatcaataaa ttagaagccg
      901 aatttgggtc tggtgtatct gatccattta tttccggatt agtagccatg cgtgcccaaa
      961 atatcaatta ttactctagt ttgatggaaa atgcaccgga tgattttgat tttggcgtga
     1021 tgcagctccc agaaaaagaa gaaggttcag gccattggtc gtggggtggc ggattcgtct
     1081 tggaagtacc atacggagca aaagatcctg aggcctctta tgaattcatc aaatatcttt
     1141 cttctccaga agtacaagaa aaattcggcg aaaaaagttt tgatatcatg gcgagcaaga
     1201 cagccaatga gaatttagtg aataatgaca acctaacgga taaaggaaaa atgatctatc
     1261 aaatggcgga cgaaaacttt aaaaatacgg tgatcacacc agttccttta gatgctccgg
     1321 attacacgac attagtgaat gaacagattg accagattct tttaggaaca aaatcaccaa
     1381 aagaaggatt ggcagatgca caaaaagcag tagaaaactt agtcaagcaa aatcaataat
     1441 ccatttttga ctgtctaggt acagaggagg caagaaaatt tgaagcgttt atctatccga
     1501 aagaaaaaag aagcaagatg ggcctattta tttatctcgc cttttattat cgggttcttg
     1561 gtatttatgc tagggcctat gcttttttca gttattggta gtttcaccga ttataatttg
     1621 acatccaaga tgaatttcat tggtctagca aatttcaaaa gaatgttcgt tcacgatgat
     1681 ttattttgga aatcgttata caacaccgtt tatttcgtca tcttcaatgt accgctgacg
     1741 acgattttca gtgtgttttt agccacagta ctcaatcaaa aaatcaaagg aatcagtgta
     1801 ttccgtacag ctttctattt accggcagtc ttatcaggag tagcagtcta catcttatgg
     1861 atgcagctgc tttctccatc tgcaggattg atcaacacat ttctgggctg gttccatatc
     1921 cctggaccag cttggctgtt tgatccacaa tggacgaaac ctgcgttgat tttgatgaaa
     1981 atatggagtt ctggaggagc aatgctactt tacttagcta cattacaaaa tgttcctaga
     2041 tctttatatg aatcagcaga aatcgatgga gcaggcgttt ggggaaagtt caaaagtatt
     2101 actttgccat taatcactcc tattattttt tacgatgtga tcacctctac gatcggttct
     2161 ttccaaattt ttcaagaagc ttatgtcatg acgaaaaatg gaacaggcgg acctgcaaat
     2221 tctttgcttt tttataatct gcatatgtgg aacaaggcgt tcgtagcttt tgatatgggt
     2281 tatgcgatgg ctatgtcaat gatcctgttt ctgatcgtgt tggttctgac gtttgtcaat
     2341 ttgaagcttg cacctaagtg ggtctattat tctggaggtg acggcaaatg atggattatt
     2401 tcaaaaagac cagtgtgaat cggcaaacaa aaaaatacta agttatgtcc tcatgacagt
     2461 aataggaatc attttgatca ttccattgct ttggatggtg tttacttcat tgaaaccaat
     2521 ggaagaaatc gttcgttatc ctccgacctt tttcccagaa aaaatcgtat gggaaaacta
     2581 tctggatacg attgccgcct ttcctttttg gcgctatgca agaaatacat tattcatcac
     2641 tgtgctagta gtgatcggta atgtcctttc aaattctttt attgcttatg gatttgcgaa
     2701 gcttgatttt cctgggaaaa agctgatgtt tgctttggtt ctttctacga tgatgatccc
     2761 ggggtttgta acgatgatcc ctcaatatgt attattctcc aagatcggct gggtaggtac
     2821 ttatttgccc ctgatcgttc catcgttctt cggcaatgcg ttcaatatat ttctgatgcg
     2881 tcaattttac ttatcgatca ataatgaatt gatcgaagct gctgaaatcg acggagcgaa
     2941 tcatttgtat atttggagtc atttgatgct tccattgacg aaacctgcct tgattaccat
     3001 tgcgatcaat tccttcaacg cagcttggaa tgatttctta ggtcctctgt tatatatcca
     3061 agatcaagaa aaatacacgt tacagattgg cttgcaagtt tttcagaacc aagcaacgac
     3121 acaatggaat tatctgatgg caggagcaac acttgtcttg atcccgacga ttttgctgtt
     3181 cttttttgca caacgttatt tcattgaagg gatggatttg acaggaggtt caaaaggatg
     3241 atgaatcgat cgattggtaa aaaagccaat gattatttct tagaaatcat tgaatggatt
     3301 ccacgtctta tccatttaca gctactatgg ttcctgtgcg tcttgcctgt tttcacatta
     3361 ggaactgcaa gtcgaacagt tctgtatatg atttatcatt acaaaaaaat gaagagaaaa
     3421 agctcggttc actattctgg aaaaagtttc gtgagaactt ctctctttat ggaaagcaag
     3481 atagtttagc aagtgtttat tttcttcttc tattgatcga ttatcagatt ttccgctatt
     3541 ggggtggggg aattggctat acattgatgt atactacgtt gtttttgctt ctattaagcg
     3601 cgcttctctt tagttacaaa atcttgcttc aactggaaca aaaagaagct tcttggatta
     3661 ctgcattttt cttgtttttt aatgatttca aaagcgcgct tttctatttg gcaggcacgt
     3721 tgatccttct tgtcgcattc tggtttgcag gaccaatcta ttttgcttta ttaggtttca
     3781 gtttcttgtt ttattttcaa gtcctgcttt ttatccatcg tacgcaagaa aaggttctta
     3841 aaaaggagga ataacatgtt gcgtttgatt gctaaaatcg atgatagtat tatcaacaag
     3901 ttcgccaagc aacggaaaac ggatagtcga aaaatcaatt ttttgaaatt tatcagtaat
     3961 atctctaatg gtggttttat ttggttgctc cttgctgctg ttttagtctt gatacctgat
     4021 actagagaat atggtctagc cattcttatc ggcagtttga tctatttttt tgttggtttg
     4081 ctttttatca aaaaaacgac aagacggaaa agaccttatg aaaaaaatcg tcggcttgtc
     4141 ctttacacaa gtaaaccgaa gacttcttct tttccttcag gacatacagc gctagccttt
     4201 ttatcagtag ctgcgttgtt cgaattaagt ccagttgcag gaatattagc ctttttattt
     4261 gcttgtttga tcgcttattc gaggattttt ttacttttac attatcctac tgacatttta
     4321 ggaggagcta ttttaggaag tgttattggc atcctatgcg ctgccgttat ctgaattaat
     4381 tgtacgaata aagcatgagt gcgtaaatgc gcatttgtgc tttatttttt tgtttcgagg
     4441 aaagcgtagt tgtttattca tttgtcttaa taacttaagt tt
//
LOCUS       DBKOIJ010000184         4472 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000184 DBKOIJ010000000
VERSION     DBKOIJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.188"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(456..881)
                     /locus_tag="ACV5NJ_002571"
     CDS             complement(456..881)
                     /locus_tag="ACV5NJ_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486602.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1255..2226
                     /locus_tag="ACV5NJ_002572"
     CDS             1255..2226
                     /locus_tag="ACV5NJ_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6486603.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2258..3655
                     /locus_tag="ACV5NJ_002573"
     CDS             2258..3655
                     /locus_tag="ACV5NJ_002573"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HHY6486604.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3814..4437
                     /locus_tag="ACV5NJ_002574"
     CDS             3814..4437
                     /locus_tag="ACV5NJ_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486605.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1562 a          719 c          913 g         1278 t
ORIGIN      
        1 tactacgtga tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga
       61 aataaataaa tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg
      121 atgaagcttg atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag
      181 ttttgcttgg atttgtttat cttcttctac tttttacata attttgaaca ggataagatt
      241 caaatgtttt aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc
      301 cttggtcatt aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac
      361 tatcgatcat ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag
      421 aaggggagaa acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc
      481 tcatccttca aacggtaatt accgattttt gtaaatatga ctgttgtttc ttttttattt
      541 tcttttcctg aaataatata ttcgtcatct ttggtagtga tcttgatatc tgtcagcact
      601 gttttttctt cagatgattc agaagaatca gttccttgtg tctgatacgt gcgtgtccat
      661 tccatttttt tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact
      721 tttagcgtat atacactgtt ggtggtatct gctttttccg cttttaagac agaagaatcg
      781 gttgtttcgt ccgaggattg gtcagagccg ccaaacaaat cacagccagt caatgtcaaa
      841 agtcccgcag acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat
      901 aggataaaga aattatacca ttggaatgta aaacttcaaa cggatggaag agaaaaacgc
      961 acaaaacacc cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaatccg
     1021 ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa
     1081 aatgaaccgg ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact
     1141 gaagacttta gttatatgga tattgatgac aagcaacact atgcaaagaa tgcggtcatc
     1201 ataatcgaac aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca
     1261 tcgatccgtg atgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg
     1321 aatggtcagc aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg
     1381 gactataaag aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga
     1441 cttttgataa acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga
     1501 gtagctgaag aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa
     1561 attgaacaaa aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta
     1621 tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca
     1681 ttgaattgtc cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt
     1741 gtagcaaaag tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct
     1801 gccactcaaa cagactacgt agcaagagaa cgtttagcag gagccaaaga agcggtccaa
     1861 aatgatccat cgatccaatt agaggtcttt acaggagact attctagaaa aagcggctat
     1921 tacggggcaa aagcattatt agaaaaaggg gcaactgcta tttttgtcag caattacaat
     1981 atgtcgattg gtgccatcga atgtttcaat caagaaggtg tcagaatcgg gaaggatatt
     2041 gcgttcagtc actatgatta tttagataaa aacgatctga gtatccttcc gaaaatcacg
     2101 atcacgccac caacagataa aatcggggaa cgagcagcag aacagctgtt aaaacgaatc
     2161 aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg aaaatatcat acatggaatc
     2221 aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc
     2281 aaatttttgg tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggcga
     2341 tggaaaagga gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga
     2401 tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag tatcaagagg atgtacagtt
     2461 gatgaaacag actgggcata attcatttcg aacatctatc caatggagcc gtttgatacc
     2521 tgatgaatct ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga
     2581 gttgattgaa aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta
     2641 tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc
     2701 caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa tggtttactc ataatgaacc
     2761 aatcgttcca gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat
     2821 gaaaaaagcg atccaagttg gctatcatga agcgttggct tcagcgttag ccatcaaatc
     2881 ttaccatgaa atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta
     2941 tccaagagac gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt
     3001 caatcgttca ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat
     3061 agtaaaagaa cttgatctca tgcctgtgat ccatgcagag gatttgacgg ttatcaaaga
     3121 aaatacagta gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga
     3181 aacaccaatc gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat
     3241 gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga
     3301 tattttgatc aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg
     3361 tatgggtgta gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta
     3421 tcggatcgaa tttgtccaat ctcatctgaa atatgtccat caagccattc aagaaggtgc
     3481 caattgttta ggctatcata tgtggacatg tatggataac tggtcgtgga ccaatgcata
     3541 taagaatcga tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa
     3601 aaaatcgggt cattggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag
     3661 aatagttgag agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc
     3721 aattatttac gatctttttc aacttttttg tctttctatt tcattttttg tatataatag
     3781 aatagtcaaa attaaatagg agtggaggaa agcatgagga aaaaaagacc gtttcttctc
     3841 ttttggttat tcggtttttt cttactgctt attttatccg cagcattttc aggaaaaatg
     3901 acagcagatc ttgctcagaa agaaacagaa aaaatcgtag caagcaaaga atcagtagat
     3961 aatttcaaaa aagaattctc aacttattgg tgggcggagt ataaaataac tgccaattac
     4021 gaaaaaggcg aattggacat ttatttgcca aaaggaaaag aagaaaatca atttattaat
     4081 atcgatgacg tacttagtgc gatttctctc agacaatatc aatttaaaga caagcatttg
     4141 accgttttgc ttttgactca tgatggcgac aaagtagccc gtttgaaaaa tggaaaagtg
     4201 atcaccgctt attctgaaaa agtagacaaa aaagtggtcg ccgatcgttt agcggaatgg
     4261 aaaaagacaa atgaacaagt caagaaaaca actgtctctt ctcaaaaaaa cgagtcgaca
     4321 ggaacaagtc cttcagattc tggcagtgga caaacgataa cgggaagtga agaaaatcaa
     4381 gcctcaatta ttacgaatga gtcatcacaa ggacaatatc aatcctatgg acaatgaaaa
     4441 acagaggttg tgaaatcagc gttttgacct ga
//
LOCUS       DBKOIJ010000185         4459 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000185 DBKOIJ010000000
VERSION     DBKOIJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.189"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            211..1917
                     /gene="efbA"
                     /locus_tag="ACV5NJ_002575"
     CDS             211..1917
                     /gene="efbA"
                     /locus_tag="ACV5NJ_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HHY6486606.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2075..2977)
                     /locus_tag="ACV5NJ_002576"
     CDS             complement(2075..2977)
                     /locus_tag="ACV5NJ_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHY6486607.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2974..3666)
                     /locus_tag="ACV5NJ_002577"
     CDS             complement(2974..3666)
                     /locus_tag="ACV5NJ_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HHY6486608.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3709..4350)
                     /locus_tag="ACV5NJ_002578"
     CDS             complement(3709..4350)
                     /locus_tag="ACV5NJ_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HHY6486609.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1367 a          955 c          771 g         1366 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgataccta
       61 aaaatttgga tttcaacgat agaaacactg ttttttatgt aattagtcca agatttttct
      121 agacttttgc tttactattc ggccaaattg gtttttcttc tttttttcgt tataatgaat
      181 atacttatga aaagaaatga ggaatgaacc atgtcctttg atggtgtatt tacccacgcg
      241 atggtcaacg aattgcgtga aacgttacta tctggccgga tctcgaaaat ccatcagcct
      301 tatgaaaatg aaatcgtgct ggtgattcgt tcacgcggca aaaaccatcg attattatta
      361 tctgcgcacc caagttatgc tcgtgtacag attacccaaa tcgattatca gaatcctgat
      421 aatccgccta acttcgtgat gatgcttcga aaatatttgg atggcgctat tttagaagac
      481 atcgaacaaa tcgaaaatga tcgagtgatc catttccact ttgctaaacg aaacgagttg
      541 ggtgatctac aaaatatcat attgatcgtt gaattaatgg gacgtcatag tacgatcgtc
      601 ttagttaatc gagaaactgg aaaaatactg gatgcgatca aacacattgg aagctctcaa
      661 aacacttatc gttctttgct tcctggtgta gaatatgttg ctccgccaaa acaagaggtc
      721 ttgaacccat ttagctccga gaaagaaaaa atctttcagc ggctttccca aactgaacta
      781 gatccaaaag caatccaacg tcagtttcag ggaatcggtt tcgatatcgc tcaagagctg
      841 accaaaagat tactggaacg tcctaatgaa aagatggttg tatgggacga atttttctca
      901 gccatcagcc atcagcctat tcctactttt tacgaaacgg aaaataaaga ttttttcaca
      961 ccgattgctt atcaggtatt gtccgaacag gcatctgctg taacagctta tccaacactc
     1021 agtcaattat tagatagcta ttatcacgaa aaagcagaga aagatcgggc aaaacaacaa
     1081 ggcggagaac tgattcgaaa aatcgaaaat gaactaaaac gaaataaaaa taaattgaaa
     1141 aaacgtgaac aaacactcaa agaatccgaa aacgctgaga attaccgtag aaatggcgaa
     1201 ctacttacta cttttctgac acaagtacca cgcggcgcaa aagaagttgt tttacctaat
     1261 tattatgaag aagatcggcc aatcaaaata gcactagatc cggcattgac accaaatcaa
     1321 aatgcacaga aatatttcca tcgataccaa aagctgaaga acgctgttaa gctgattggc
     1381 gaacaaatcc aagaagccaa agatgaaatc caatatcttg aatctgtctt atctcaactg
     1441 gaaattgccg gcccaatgga tatcgaagca attaaagaag aattgacttc agaaggatac
     1501 ctgaaaaaga agaacttaaa aaaacaaaaa cgtaagaaac catctcagcc tgatcaatac
     1561 ttttcttctg acggaacgct cattcttgtt ggtaaaaaca atcttcaaaa tgaccagctg
     1621 acaatgaaga cagctaaaaa aaccgactat tggcttcatg caaaaaatat acccggttcg
     1681 cacgtcatca ttaaaagtga caagccctca gatgaaacaa ttacagaagc tgccgagtta
     1741 gctgcctatt tttcaaaata ccgatactct gcccaagtgc cggttgatct tgtacaggta
     1801 aagcatatcc gaaaaccaaa tggcgccaag cctggttatg ttatttatga aaaccaaaag
     1861 acgattatcg tgacaccaga agaagaaaaa attacagcta tgaaacaaaa caggtaatca
     1921 atgagtgttt ctagaatcat atttgcgtac tttcccacaa cagcaattag ttataaattg
     1981 tttgctacaa aaagaggagt tgtgacaaaa ttctgtcaca acccctcttt tttgatcaac
     2041 ggtgttcaat aaacagcgtt ctcatctgct tttcttagct ctgctggtcc agtaaccgag
     2101 taagctcttt gataaatctt tgagcgattt cactgagcgg acgtttcgcc gaataaatga
     2161 tataaaattc atttagttcg ccttctgcca aagggattaa ccgcaagtgc tcgcgaatcc
     2221 ctccaatcac aataccagat cctgaagcgt aagcatcagt ttcaagaacg atattcatca
     2281 aagtacctcg atcactcgta taaatcactg tttcttgatc agggatctcg ataaggtctt
     2341 cataaaaata cgggaaatta tttccatcct gtgtaaaacg aacttgagga taagataaca
     2401 attcttcttt ggtgatctct gcgtgttcag ctaatggatg tccttgcctt aaaaaaatat
     2461 gcgttttgaa ttttccaatg ctgcggccac ttagctctcc ttgttgtaaa tatcttctga
     2521 taccagcttg attatgctca ttcatataaa taatgccgat ctcactgtgc tgctctttga
     2581 cttcttcgat cactctcgct gttgtcgtct ccatgatccg aaattgtttg atctcgtctt
     2641 tgaattcctc gagtagctgt gcggtcaaag gacctaagaa atcataatgc tgggcagcga
     2701 tcgaaaaatt gtctctcttt tcactcgtta gatagtgagt ttctaatgta tcaagctgga
     2761 ccaatatttt ctcggcacga tttaagaagt cttgcccttc ttctgttaaa tatgcccctc
     2821 gattcgttcg agtaaacagc gtgatcccta actcggtttc taattctttg ataccagctg
     2881 aaagacttgg ctgggagaca aacattttct ttgccgcttc tctgaaacta ccattgtttg
     2941 ctacagcgac cacgtatttc atctgctgaa tattcattgc tcaaactcct cgaaataata
     3001 aatttctctt ccattcaagg gctggattgg tcggttgttc ggtcgagcat actgaccatc
     3061 gaaggggaga agaaactctt ggttcagttt tcctacatga tccattacaa accattggat
     3121 tggcccgctg gtaggcggag ttgtcaatga gccaatataa tggaaatggc ttttttgctc
     3181 cggaaagaac acatccggat ttacccaatg ctcatgtttt tctaagttcc aactttcccc
     3241 attttcttga ttatacagcc aaccttcctg taccattagc tggtacatga cacccacaac
     3301 tagattctct tttttgctgt tcatatgaac aaaatgaaat tcaatatctt cctgttttcc
     3361 gttgatgata tgttcagaag gcaagtggaa atgaatatct gtcaaataat agcgttcttt
     3421 cttgaactct acataactga gttggtcaaa aggtactaaa tgaatcgtgt ttttgaattc
     3481 cttgtcagta aaccgttgct tttgataatg aaaggaaagc gtgtctcctt ctattttggt
     3541 ttcttccccc ttggtcaaat gaataggcga ctgcaatgga aattccgcac ctctcgcaaa
     3601 ccaatcacat aaactcgccc aattttccgg acctttgtct ccagaatagc cccattcgac
     3661 atccatattc tttttcaact atacttcccc ctgcatctca tatcattttt atttttcttc
     3721 tttctttaca atcgttgaga cggtttctaa aaacgcctct tttccataaa attcctggtt
     3781 caatagttcc ggaaatatct ctttaaagaa atactgaaca gaaggccgat gttcaaagct
     3841 tttaatcgta gtcactgcct caatgaacat ctccttataa actttttccg ggttattttt
     3901 gatgatttca tcaaaagatt cataaagaag gtcaacctta tctgaaatgg ctaatagttc
     3961 tccttctacg gtttcatctt ttccttcgaa caaccgacgg cgataaactt cttggaactc
     4021 agctggaatc tcttggttga tgaattcgtc cgtcatcttt tcttcaactg tttgcagcat
     4081 ccctcttaac tctttgcttg cgtatttcac cggcgttttg atatcaccaa taaaacgctc
     4141 tgtgtagtca tgattcaatg atttttcata gagcagtttc caatcgatcg gttgtccatt
     4201 cgcttcttca atgtctccta gaacttgcgc aatagatgct acacgatacg aatgtgctgc
     4261 aactgtatgc tctgtatatt taaaaaatcc aggtgccctt gtgatcgtct ctaaattgtt
     4321 caatcctaaa ataaattcat tcaaccccat aaaagttacc tccctttgag atttcttttt
     4381 ccgaatatca tatcattaaa taactgatat tcgcaacatt tccgctcttt accatttttg
     4441 aaacagctcc cttaccaaa
//
LOCUS       DBKOIJ010000186         4425 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000186 DBKOIJ010000000
VERSION     DBKOIJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.190"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..259
                     /locus_tag="ACV5NJ_002579"
     CDS             <1..259
                     /locus_tag="ACV5NJ_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486610.1"
                     /translation="SKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLR
                     SNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1371..2339
                     /locus_tag="ACV5NJ_002580"
     CDS             1371..2339
                     /locus_tag="ACV5NJ_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHY6486611.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            2349..2837
                     /locus_tag="ACV5NJ_002581"
     CDS             2349..2837
                     /locus_tag="ACV5NJ_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HHY6486612.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            3133..3414
                     /locus_tag="ACV5NJ_002582"
     CDS             3133..3414
                     /locus_tag="ACV5NJ_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486613.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            3416..3832
                     /locus_tag="ACV5NJ_002583"
     CDS             3416..3832
                     /locus_tag="ACV5NJ_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486614.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1655 a          763 c          740 g         1267 t
ORIGIN      
        1 ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata aaattcaaat
       61 cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa caaataattt
      121 actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg attctccaaa
      181 aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta aaccaattga
      241 attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa tttcaccgaa
      301 ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt tttgtattgt
      361 ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac aagcggttat
      421 ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc cctttagtgt
      481 gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt cctatggacg
      541 tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca atccttctaa
      601 aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg aatccaaatt
      661 tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta ggtaacatta
      721 gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt tctggtatgt
      781 aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc tgtagaacgt
      841 tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac aagataaggg
      901 attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc taaaaggact
      961 ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt aatgtgagag
     1021 cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta ggaaatttcg
     1081 cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg cccccctctt
     1141 ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta tccttactct
     1201 cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct
     1261 gtttttaggg gtctttttgt ctgtttttag gggtctttaa ttagggggtt gacccccatt
     1321 agaaagctct ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg
     1381 aaattgtgaa ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag
     1441 aaatgaattt attcttctct ataatatcta gaatgcgtga tgtaggcgat aaaaaagtac
     1501 aatttacatt cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat
     1561 tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa
     1621 gtgctgacgg tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa
     1681 aagctgatat tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata
     1741 atcttgaaga atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt
     1801 ccaaaaccat gtttagatta ttaaaacaat acagaaccaa aggaattgcc aatttttcaa
     1861 aagaagactt tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata
     1921 aaaaaattat aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ttgacaatcc
     1981 aaaagagata cggaaaagga cggggaaagc ctgtcattgg ctatcaattt acatttaaac
     2041 ctgagataaa aaaatcagac gactttaaca aacatgctcc aaccaaagaa gaacaaaata
     2101 aagctttgga actagctaat caacaggttc aatctgaaaa agaacctact tcattacaag
     2161 aaaaaatcaa agaacaaaaa cgaaaattag aacttttaca acagttagca gaattagaaa
     2221 gaaaacaaaa taccccaagt aagaaaatta acaaacaaat agcagataac aattccgata
     2281 ttaccccaga atttaaagaa aacttactag ataatctcca aaacttattt aaagaataag
     2341 gggtttttat gacagataaa actatcaaag aattagcaga tattctaggt gtttcaaaag
     2401 ataaagtgaa gtatcaactg aggaaattac ctagtaattt tacctacaaa aaaggaaata
     2461 ttacctacct gaaaaaagaa gcagtaacaa caattacaaa ggttttatca ggtaatgaat
     2521 cgggtaactt acctagtgaa ttacccgatt cattacctac cgattaccta ctcaaggaga
     2581 ttgaaactaa gaataatcat attgaagaaa aagataggca aattgaaaaa cttcaaatag
     2641 ctttagaaac gcagcaaaaa ttactcgacc agcaacaaca actcacccta caagccaatc
     2701 aacaaatcaa aaaacttcac gaacaactcc agctaaccta tgcagaagaa tcttcagaaa
     2761 aagaacccct ttctgttact gaagaaactc aaaaaggaca aaacaaggag aaaaaatggt
     2821 ggcaattctg gcaataaccc aacaattttt attcataaaa attgttgggt tactttcttt
     2881 ttttgctttt acaagcatag cgacatgatc tcacgtcgct attttttaaa gagcataagg
     2941 aaacgcggta tcactttctt acgaaaggtg ctaggtaggc tgtgcctacc acgtttctaa
     3001 gaaaaaaatt tgctatcact ttttcgtcat tgtggatagc ttatgtatag tgtttcggca
     3061 aaaaacagct atacaaatac tatatttgta tgatatacat aaattataca caaatgcgaa
     3121 aggaattctt ctatggacat tttagttcgg aatattgatc caaaagttat ttcagaaata
     3181 gatagacgtt gccaagaact cacaacaaaa actggcaaaa aatggagccg taatgcgtac
     3241 ctaaaagttc taatcgaaaa cgattttgac caggcactta ggcattacaa aaaagatcaa
     3301 ttcgatttat tactcgaaaa atttatcgat atacaagcaa aaaatactga aacattgaac
     3361 gactatgttc atacaaacaa ccaactcatt gaaacactta tcggaggaat ataacatgag
     3421 aaaaacttct gttcaaaaac acgttaccct ttatcccagt cacattgacg ttttagattc
     3481 catcatcgaa aaaaatccag atatcaataa ctactcggaa gccattcgtt ttctatgtat
     3541 gagttatgaa cctaatacta atacgacaac taccagcaac caagcaaaac taaatgcgat
     3601 gggaaaagaa atttctatta tgtccgaaat cattgcagaa tttgctgatt tatatctaaa
     3661 agatactggt gttcttatcg gtacaaattc taaagtatat caagaagtga aagaaaaaat
     3721 tgaaaatcaa ctcaatccct cccgacgaaa tcaacccaaa aatcgtcaaa aagaaaccaa
     3781 tcaaacattc acttctagaa attttgaccc aacaaacttt ctaaaaagat gaaaaagaaa
     3841 ccgaaaattt tttctatttc tcagtttctt ttttaggggg cagtcttcca gcaagatttg
     3901 caagttaccg gtaacttgca caaaaaaaga aaaatccgct tgtgcgaatc cttctttttg
     3961 cattttgggg cgctgcccca atctccaaat atcggttatt taatagcgtg gtaactcaaa
     4021 aaaatagaac gtactctgaa ataaaaaaag actgctctaa cgagcagcca ataaaaaata
     4081 atgagcgttt attcttgaaa aagcagcgcg cttatgaagc gctgccaaaa atttaccata
     4141 ttttatgaaa aataacaagt ttctaaaaag gaatcggtta agattcacaa agaatcgtaa
     4201 acagacaata gcggaaaatc aagcttccga acgtaccttt tagctagacc aatgaaaaat
     4261 cacaaagtga gcccctgtat cggttgatac aagggctact tggcatactc gacattatgt
     4321 ctactagctc gataaataac gattatgttc ccaaaacaat attttatgaa ctataacaaa
     4381 aatttgttat cgcatattgt atattttaat tataggtgta tatca
//
LOCUS       DBKOIJ010000187         4388 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000187 DBKOIJ010000000
VERSION     DBKOIJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.191"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            41..271
                     /locus_tag="ACV5NJ_002584"
     CDS             41..271
                     /locus_tag="ACV5NJ_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HHY6486615.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            368..1669
                     /gene="murA"
                     /locus_tag="ACV5NJ_002585"
     CDS             368..1669
                     /gene="murA"
                     /locus_tag="ACV5NJ_002585"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HHY6486616.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1682..1858
                     /locus_tag="ACV5NJ_002586"
     CDS             1682..1858
                     /locus_tag="ACV5NJ_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HHY6486617.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1993..2289
                     /gene="yidD"
                     /locus_tag="ACV5NJ_002587"
     CDS             1993..2289
                     /gene="yidD"
                     /locus_tag="ACV5NJ_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HHY6486618.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2353..2736
                     /locus_tag="ACV5NJ_002588"
     CDS             2353..2736
                     /locus_tag="ACV5NJ_002588"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6486619.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            2905..3789
                     /locus_tag="ACV5NJ_002589"
     CDS             2905..3789
                     /locus_tag="ACV5NJ_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HHY6486620.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3903..4139
                     /locus_tag="ACV5NJ_002590"
     CDS             3903..4139
                     /locus_tag="ACV5NJ_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HHY6486621.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4227..4307
                     /locus_tag="ACV5NJ_002591"
     tRNA            4227..4307
                     /locus_tag="ACV5NJ_002591"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4261..4263,aa:Tyr,seq:gta)
     gene            4314..4385
                     /locus_tag="ACV5NJ_002592"
     tRNA            4314..4385
                     /locus_tag="ACV5NJ_002592"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4346..4348,aa:Gln,seq:ttg)
BASE COUNT         1487 a          687 c          979 g         1235 t
ORIGIN      
        1 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
       61 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      121 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      181 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      241 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      301 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      361 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      421 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      481 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      541 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgacgc
      601 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      661 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      721 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      781 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      841 gatttattta gatttcccta gtgtgggtgc tactcaaaat atcatgatgg ctgccgtaaa
      901 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
      961 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1021 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1081 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1141 ctctgaacat aactgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1201 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1261 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1321 tgcagaaggt acaagtgtgg tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1381 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1441 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1501 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1561 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg tcgaacgtgt
     1621 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1681 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1741 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1801 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1861 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgataaa
     1921 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     1981 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2041 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2101 gcaattcaaa cccatggtgc gctaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2161 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattccg attaacgcga
     2221 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gggtagcact
     2281 gagcgataaa gaaaaagagg aaatttattg tcaaagtaac aactccgcaa ctacttatat
     2341 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2401 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2461 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2521 gaaatcgtca atgtagcagt aaccccaacg tatcaaggaa aggggattgg cggaaaatta
     2581 cttgagacag cttttcagaa gctttcacag ctagcttcaa accaaactcg gatcattata
     2641 cgtactggca ctactagtag cgctgcgtta cacctctatc aaaaaatggg ctatgtggag
     2701 attggtcgag tcaaagacta ttttatccat cattaatctg agcccatttt tgaaaatgga
     2761 gatcaattaa gggatcaagt gattttagca attcacttac ctaaaaatag ctttaactaa
     2821 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     2881 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     2941 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3001 gaatcttggg aagaaatgaa aacgcaatta tttcaagttt ataaaaaaat actcgtttct
     3061 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3121 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3181 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3241 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3301 attggcgggg cgttgttcac gaaaggacaa ataaataaag gggcacatct ttacggtgga
     3361 gaatttgggt tgatgttctt agatggcgat aagacattca gtaaacttgg tacagcggta
     3421 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa
     3481 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3541 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3601 gtattaggcg gcggggtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3661 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgag
     3721 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaagga
     3781 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag
     3841 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     3901 aaatgatgtc aaaaaaaaat aaagatattg aagtgcgtat agaagaagtg aaaaaagata
     3961 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4021 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4081 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4141 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4201 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4261 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4321 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4381 cccagtat
//
LOCUS       DBKOIJ010000188         4377 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000188 DBKOIJ010000000
VERSION     DBKOIJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.192"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            73..234
                     /locus_tag="ACV5NJ_002593"
     CDS             73..234
                     /locus_tag="ACV5NJ_002593"
                     /inference="COORDINATES: protein motif:HMM:NF012267.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HHY6486622.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACV5NJ_002594"
     CDS             253..1530
                     /locus_tag="ACV5NJ_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6486623.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACV5NJ_002595"
     CDS             1540..2196
                     /locus_tag="ACV5NJ_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486624.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACV5NJ_002596"
     CDS             2196..3572
                     /locus_tag="ACV5NJ_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HHY6486625.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACV5NJ_002597"
     CDS             3702..4115
                     /locus_tag="ACV5NJ_002597"
                     /inference="COORDINATES: protein motif:HMM:NF013068.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6486626.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACV5NJ_002598"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACV5NJ_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DBKOIJ010000189         4371 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000189 DBKOIJ010000000
VERSION     DBKOIJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.193"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..791
                     /locus_tag="ACV5NJ_002599"
     CDS             <1..791
                     /locus_tag="ACV5NJ_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HHY6486627.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            888..3137
                     /locus_tag="ACV5NJ_002600"
     CDS             888..3137
                     /locus_tag="ACV5NJ_002600"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HHY6486628.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3140..4315
                     /locus_tag="ACV5NJ_002601"
     CDS             3140..4315
                     /locus_tag="ACV5NJ_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6486629.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
BASE COUNT         1523 a          626 c          803 g         1419 t
ORIGIN      
        1 tttgtgggta ttcaaaaaca gaacctagac atagttttgg accagctatt cgagaacaat
       61 acttaataca catagtgcta gaaggtgaag gctattattc aattaatgat caaaaatatt
      121 ttctgaagaa aggccaagga tttgttatcc caccaggagt atctactttt tatcaagcag
      181 atgaaaaaaa tccgtggtca tatgtatggt tgggtttaag tggcaatctt gtagaatact
      241 atctaaaaga aattggtgtt ggaaagaatc atcttacctt taaagtcgaa aatttaaatt
      301 catttaaagg attaattttt gaaactttat cgtatgatca ggacaatcta gtcagtgaat
      361 tgtccagaca aaaacaagtt tataaatttt tagaactcct cttaaattca gctagttatc
      421 tgaacgaagg gataattact aaaaagatga atccttttgt tcgtcaaaca attgaaataa
      481 tacgtgagaa tgcgtatcaa aaattatctg ttaaagatat tgctgtcagt ctatcgatac
      541 acgaaaatta tttatcacga ttgtttaaga atgatatggg aatatcaata aaagagtata
      601 ttagtgaaat caaaatttct attgcaaacg atctgttatc atcaactgaa tattcaatcc
      661 agcaaatcag tgacaagata ggattctcaa cagtacaatc tttttctaaa gcctttaaga
      721 aagcaaatgg tgtttcaccg ggagtatatc gaaaacgtag attaggtcta ggaatcatta
      781 atggtgaata aactaaacat ttgattttga taaatattcg ttactttaaa cgcttactat
      841 ttatgtgagc gttttttata atgcaataaa gaaacggagg agagcttatg tataaggata
      901 atgaaatgaa tattatttat cgatcagaaa accaacaatt tcatttgttt aatcaggaga
      961 taagttacat ttttaagata tcggaagatg gtaaattatt gcatttatat tatggtgaga
     1021 acttaccaga taatgactat agtcatctaa ttgagatgca tcatcggcct atgacaacct
     1081 atagaaatga aaacgatttg ctatattcgt tagagcactt aaaacaagaa tttcctgaat
     1141 atggtacaac agattttaga catcctgcta tttcattact acagaaaaat ggctctcgct
     1201 tatcagattt tgtctatcaa gggtacgaag tggaagaagg aaaacctaaa ctagaaggtt
     1261 tacctgctac ctatgttgat aataatgatg aaagcaaaac attgaaagtt tatattttag
     1321 attcagttac aaatgttcaa gtagaattat tatatacaat ttatagagat tatcctgtta
     1381 ttactcgtag cgcgcgagtt atgaataaag gaaaagatat tcaaataatc gagaatatta
     1441 gcagtttatc attggattta cccgatgcaa attatgaatg gcaacaatta tctggtgctt
     1501 ggggtagaga aagaactatt aagtcgcgta ctttacaaca aggaattcaa tcaatcgaat
     1561 ctactcgagg tatttcaagc catcagcata acccatttgt tactttaaaa agaaaaaata
     1621 ctgatgaaaa tcaaggagaa gctattggtg cagcactagt ttactcagga aacttcttaa
     1681 ttcaagcaga agtagataca tgggatgtta ctagactaca agttggcatc aatccatttg
     1741 gatttaattg gaaactcaat ccaggagaaa tatttacagc accagaagcg attctggttt
     1801 attcaagtaa gggtttaaat gccatgagtc aaacttttca tcaactattt agaaaacgct
     1861 tagctcgtgg taagtggcga gaaaaagacc gtcctgtctt aattaataat tgggaagcaa
     1921 catattttga ttttgacgaa gaaaagttgg taaatattgc taaaaaagca tttgatgttg
     1981 gtattgaatt atttgtatta gatgatggct ggtttggaga aagagaaaac gattgtgcag
     2041 gattaggaga ttggcatgta aatcctaaaa gactacctag aggaatcggc agcctttcag
     2101 aaaaaattcg tagttttggt atgaaatttg gtttatggtt tgaacctgaa atggtgaata
     2161 aggatagtga gttatatcgc acacatccga attggattat ttctacacct aatcgaaaac
     2221 ctaatcatgg taggaaccaa tatgtattaa attttgggtt agatgaggtt gttgaaaata
     2281 ttttcgatca aatgtgtaaa atcattgatg aatcaaactt agattacatt aagtgggata
     2341 tgaatcgtcc attgactgat gtatttgaca gtcacttgtc agcagatcaa caaggagaag
     2401 tattccacag atatgtacta ggcgtatatc gcttatatga aaaacttata acaaaatatc
     2461 ctaacatact atttgagtct tgttcatcag gtggtgggcg ctttgatcct ggaatgcttc
     2521 actatgcacc tcaagcttgg gctagtgatg attcagatgc aattgaaagg ttaaaaattc
     2581 aatatggaac tagtatgctg tatcctcttt cgtctattgg tgctcatgta tcaattgttc
     2641 caaatcatca aacaaaccgt ataacaccta taaaaactag aggaaatgta gctttttttg
     2701 gtgcatttgg atatgagctt gacttaaatg aattaagtga aggcgatttg aggattgtaa
     2761 aagaacaaat tgccttttat aaaaaatata gaaccataat tcataatggt actttttata
     2821 gactattatc accattcgat gatgataatc aaaccgcgtg gatggttgtt agtcatgata
     2881 agaaaacggc tattgttgct cattataaaa cattaaatga ggtaaatgct ccttaccgcc
     2941 gactgaaatt aaaagggtta gataatgacg gactttataa tgtaggacaa aatagtttgc
     3001 gatctggaat ggaattgatg agagcaggac tagtctgttc agatgcatct agtggacaaa
     3061 taaatgatca gaatcgacgc ccagaaacat ttgattttga ttccaagatt tgggttataa
     3121 cacaggagga aaagtagaga tgaataacca aaaaagctta cttcaaaaag gagcaatctt
     3181 aagtatttca ctaatgttaa caagctctca agcaattaat ggtgttattc cacaaatgaa
     3241 acagtctttg gggatatcac aaagtcaaag tgaactttta ggaactgcac caagtataac
     3301 tgttattttg tttatcttat tatctagtta ctttgccaaa agaataggaa tgaaaaagac
     3361 aataatttta ggtttattat tagctggtat aggaggaatt attccagtat ttacagctac
     3421 cttccaaacc gttcttatta gtcgagtaat attaggtgcc ggtttaggag tatataattc
     3481 attggctgtg acatatatca gctctttata ctctggcaat actagagcaa cgctattagg
     3541 tattagaaat tcaatggaag ctttaggtca gacaatcctt atttttcttg ctggagtttt
     3601 agtgaacatt aattggacgg cttcgttttt agtctatgca attgcgtttc ctgtagctct
     3661 cttattcgcg ctaaaagttc ctgaaattag agatgaaaat agtgacatac ctgaaaatca
     3721 tgtaaaagaa aagatgaatc caatggtttt tgccctagtt ctatttgcaa tcttactcgt
     3781 aatgaatagt attgctattt ctgtacgatt tgcatcaatt gcaactgaaa ttaaaggggt
     3841 aaactttaat gctagtaact atttagctct aatgcctatc ttaggaatac tagcaggatt
     3901 tatgtttgga ccaataaata agtggatagg aaaaggaacc ctatacttgg gaattatatt
     3961 ctttataatt tcaaatttgt taattgctgt gtcaaacggt aatatgacgt tcctgctaac
     4021 gggtttattt ttatcaagta taacaggatc atggtgcttc ccatttatct ttagtaactt
     4081 agataaagtc actacaaaga atacaattaa ttttgctact tcattgattt ttattggctg
     4141 taatattggt aattttatag ctcctattgc tatgcaatta gcacaatttc taacaagaag
     4201 tacaaatttg actgctccat tttttgtatt cgcaggaata tttattgtga tcctatttgt
     4261 aacatttttt gcgacaaata aaaaaaatac tagagtaacc cgtggtgcta gttaatttta
     4321 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac t
//
LOCUS       DBKOIJ010000190         4331 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000190 DBKOIJ010000000
VERSION     DBKOIJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.194"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..278)
                     /locus_tag="ACV5NJ_002602"
     CDS             complement(<1..278)
                     /locus_tag="ACV5NJ_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486630.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLK"
     gene            complement(486..1412)
                     /gene="glsA"
                     /locus_tag="ACV5NJ_002603"
     CDS             complement(486..1412)
                     /gene="glsA"
                     /locus_tag="ACV5NJ_002603"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HHY6486631.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1435..2862)
                     /locus_tag="ACV5NJ_002604"
     CDS             complement(1435..2862)
                     /locus_tag="ACV5NJ_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHY6486632.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2893..4107)
                     /locus_tag="ACV5NJ_002605"
     CDS             complement(2893..4107)
                     /locus_tag="ACV5NJ_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HHY6486633.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1354 a          918 c          770 g         1289 t
ORIGIN      
        1 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
       61 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      121 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      181 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      241 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      301 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      361 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      421 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      481 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      541 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      601 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      661 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      721 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      781 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      841 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      901 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
      961 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1021 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1081 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1141 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1201 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1261 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1321 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1381 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1441 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1501 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1561 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1621 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1681 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1741 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1801 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1861 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1921 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     1981 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2041 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2101 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2161 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2221 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2281 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2341 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2401 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2461 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2521 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2581 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2641 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2701 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2761 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2821 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2881 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     2941 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3001 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3061 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3121 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3181 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3241 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3301 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3361 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3421 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3481 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3541 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3601 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3661 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3721 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3781 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3841 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3901 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     3961 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4021 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4081 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4141 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4201 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     4261 gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct ttttttattt
     4321 ttaaagatat t
//
LOCUS       DBKOIJ010000191         4288 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000191 DBKOIJ010000000
VERSION     DBKOIJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.195"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            75..503
                     /locus_tag="ACV5NJ_002606"
     CDS             75..503
                     /locus_tag="ACV5NJ_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HHY6486634.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACV5NJ_002607"
     CDS             490..732
                     /locus_tag="ACV5NJ_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486635.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACV5NJ_002608"
     CDS             complement(1032..1247)
                     /locus_tag="ACV5NJ_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486636.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACV5NJ_002609"
     CDS             1354..1668
                     /locus_tag="ACV5NJ_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486637.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACV5NJ_002610"
     CDS             1772..2950
                     /locus_tag="ACV5NJ_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6486638.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(3131..3316)
                     /locus_tag="ACV5NJ_002611"
     CDS             complement(3131..3316)
                     /locus_tag="ACV5NJ_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486639.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            3353..3646
                     /locus_tag="ACV5NJ_002612"
     CDS             3353..3646
                     /locus_tag="ACV5NJ_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HHY6486640.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1466 a          646 c          824 g         1352 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
     4261 aagcaatact tcaattaatc aagtttta
//
LOCUS       DBKOIJ010000192         4284 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000192 DBKOIJ010000000
VERSION     DBKOIJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.196"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..998)
                     /locus_tag="ACV5NJ_002613"
     CDS             complement(<1..998)
                     /locus_tag="ACV5NJ_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HHY6486641.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKD"
     gene            complement(1106..3133)
                     /locus_tag="ACV5NJ_002614"
     CDS             complement(1106..3133)
                     /locus_tag="ACV5NJ_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like domain-containing protein"
                     /protein_id="HHY6486642.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3278..4006)
                     /locus_tag="ACV5NJ_002615"
     CDS             complement(3278..4006)
                     /locus_tag="ACV5NJ_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HHY6486643.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1180 a          894 c          710 g         1500 t
ORIGIN      
        1 ttgtcttttt taatcgtaac atccgtcgtg ttcaattcct tggctacttt tccgtctatt
       61 gtaaaatctt tttcaaatac ccattgattg gtatagttga tcgtagtatt tccttttttt
      121 ctcgaaaatt ttccattttc attttcattt cctaaaacag tcattgtaag gtgatattca
      181 cctggggtta gcggttgacc atttaaagca gttggaaaag aaaaatttgt attcggcgca
      241 atctgcaatc cttctttttc ctcttgatat aaaacttcag aatgtccttt ttttgtaatt
      301 tttgtttcaa taactacttg attgatatag gttttttgat cattttgaac gtttgctaag
      361 atgacatttc ttgcattgat ttgatctggt ttaacttcat gaagagtaag gttcggagca
      421 acttcgttca gtgtctgttg catcaacaaa gcaataatat aggtgtattc attctcaatc
      481 gataagcttt gatccttagt ttctgtttgg tccttttccg tctggatttc ttggaatgtg
      541 atcccaccag ccaatacacc atcaaatcgc tcattcggca tcctgacatg aaaagcaacg
      601 gtttggacac ttttaggttt tagcgtcact atctgtggat agctcacata atctaccaga
      661 tcaaagatca ggctcatgtc tttttcaatc tcgttttttc cgtattcaac catcacattt
      721 gaattggtgg ttgcgctatt cacagaaata tcgattttca cttctttttc tgtatcattt
      781 ctcaattccg cttttacttc tacttcctga tccggatcaa gttgaagatc gaagtaagtt
      841 tttctcttgt cgatttgatt ctctgaagtt atcggcgtca ctgaaaaatt aaatgcacta
      901 gctgacactg atatattgtt aaaaagtgcc cctaaaataa ctaggagtgt tataaataat
      961 tgttgttttg gtctcaagaa aatcgctcct ttaatcatat gtaagagaag actataacaa
     1021 aatcttgtct ctgtgggaca caagagccta tggtacctgt gcccttcttg aaactcaata
     1081 attttgttat attcttctct tcctgttaat ttcctgggac atctgcaagt atccaagata
     1141 gtttcccctc aaaggttccg attctttggt tttctacagg aatatttatt ctgattccac
     1201 gatgagctgt agaatctaaa agagaggaca aatcgctttc acccttttga tcactttcga
     1261 aaatcgtaac agcggatgtc cctataaccg ttgtattttc agaggtaaca aaggaaagag
     1321 cttctggaag ctcaaaatct ccatttgtaa gaactttctc ttgtttcaat tgtaatttcc
     1381 aacttccaaa agcccgttca tctataattt tcaacccacc tgaaaactgt ccgaattcac
     1441 ttaatggtct actggatatt ttatgttttc caaaattgac atattgagga gcagttgaga
     1501 gataaagttt gccttttctg taataaaaat agatatcctc tgtattttcg gagaagatgc
     1561 cttctgtatt cgctgaatcc gagtcaaaaa catacccttc aatatcaata ggagtttctt
     1621 tatacttctc tcctattttc ccagtgattg ttttcatttc agcaatatca atctgactgt
     1681 ctttatcttt ataataaaca tggatatcta tctccttcag ctttgcgatc tttaggttta
     1741 tttcattcga ttgctcaatc aaattcccgt tttcaaggta ttcaaaatgc atagtgagta
     1801 ctttttcagg aattacttcc tgcaaagttg tctccaattc gattattaac gtatctccag
     1861 caataaaata atctgtcaga tttagcgtga ctcgatttcc ggttatcaag atatctttgg
     1921 catcaatttg atttccattg atcaagatat gctcgggaat agttaattct tgtgggaggt
     1981 cgatactagt ctgcattttc ttatattcaa gaaaatcccc ctcgaccttc aatgtatttc
     2041 tgatagttat cttatcgttt cctccagctt cagcgatggc cccataatca ctatcgttga
     2101 cttttacatc agctgttgat gtgacattcg atggaagtcg cgtgacagcg acagcatttt
     2161 cttgaaaaaa agtacctgtt gaagaagtaa acccccaatt tacttctgta gaaccaaact
     2221 gcctaatgag atcaggtata atccgagaag agcgaccgac accttccaaa tcataagtaa
     2281 gacttctttg ctccgcgtcc catttaatta ttaacaagcg ccatgtgcca ttagaaagaa
     2341 atgtaggagc taccgtcacg ccagtatgct ctccagaata attattatta tttggtttag
     2401 gcgtgacaaa agcgacatgt ccatgccctc tattatcagc agttatctct cgatccattc
     2461 gatcagaaga accactattt ttatacgtat caaactcaat cgagagcgca ttcgcaatgt
     2521 aaggttggcc agaacgagta gaatatgagc caattcccat acctggcgat ccaataactt
     2581 gactgggatt actagccatt ctaggatcgc cagtcaatgt aaaggtcatg ccatctcccg
     2641 ctctatctcg ttgatttcct aagtataaat aagatttaaa tgtaaaatcc gagagtaaac
     2701 taatcttctt atttgaccaa atagctcctc tttgagacct ttctgctggg ttcaactgca
     2761 gtaacttccc attttccaat atggacgtgc tagtaccgat aggtgtttga aaaattttat
     2821 cgatagaaat aatattggga tcgtcaggca gctcaaccgc agaaattggt gcatcttcta
     2881 gtccttgttt gtccgaaccc tcagcttcta cgttgtcagt ggtctctcca ctactcgaaa
     2941 tacttaaatc ctcactgctt tcttgtgtct ggttggattc tattaaactg tcgaatgtcg
     3001 aagtgagttt gctttcttcg tctcttctat ttccatgctc caactgattc aattccaaag
     3061 agtaccctat gatcggaaag atattagtca ctaagagact gcttaaaatg agcacgatcc
     3121 tcctcttttt caatatatct tttcctcctg atcgttaaat tcctagatga actattcgta
     3181 tccaaacaaa tttagcctag cctttgacaa tttttgctcg attacctcgc aagacaatat
     3241 gactacgtat ttatttttaa cagttctttt cagtaaacta gttgttaggt gtatcttcta
     3301 atgtccaaac taatttagtg gaatattgtt gcgctaactt gacagattta ccgggtaccg
     3361 aaagctgtac cgcatcttga ttttcatttt catttgcacc aaatcgatag acccaagtcc
     3421 ccacacctgt tcctgattta gcattaattg ctaacgtatt gtttcctccc ggtgtcaacg
     3481 ttaattcaga attcaccgtt tgcggcgtat atgttggatc aaccaaagaa acagcctgcc
     3541 ctttcgtaaa tttcagctgt gctccaacta gttcatcccc actggcagtt ttaaactgtt
     3601 ctggttgaga gacacttaat ttccacccag ataaggtact tctcctatct gtcacttgga
     3661 catatgtcgg ttttctagac ccatcgttca tcacatcagc agctgcataa tacgttttat
     3721 ccgcagtaga aattttttga gtcccaaatt tgaattttga tccataatca atcgataatg
     3781 cccctcctgt tccagggctt ggaggatctg atggatcgac aggtttattg ggatcaggat
     3841 tctctggatc aacaggagga gttacaccct catctcctgc ttcaaatgta atcgttcctt
     3901 ctgtgttata ttcctttggg tatacttcag ttgcagaaac aaaagtcgta cttgctacca
     3961 tgctgaataa catcattgct gctgttaata cgatttcttt tttcatcatt gacattcctc
     4021 tttctttatt tactaaacat atcagtcgat tggattaatc catagcaaaa ttctgtttta
     4081 cgatacttat gaaataaaag aacatagacc tatatttggt cctatgtatc gattagttat
     4141 aaatgattat cagcccgatt tgatttctag tttttattgc aacttgatgc aaacacaatc
     4201 tagaaataca gctgtcatag cctttaaaat aattcgtaag tccccctcct atctatcaaa
     4261 atgtgacttg acgagagtgt aaaa
//
LOCUS       DBKOIJ010000193         4282 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000193 DBKOIJ010000000
VERSION     DBKOIJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.197"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(37..1257)
                     /locus_tag="ACV5NJ_002616"
     CDS             complement(37..1257)
                     /locus_tag="ACV5NJ_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HHY6486644.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="ACV5NJ_002617"
     CDS             complement(1277..1882)
                     /locus_tag="ACV5NJ_002617"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HHY6486645.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACV5NJ_002618"
     CDS             complement(1931..2863)
                     /locus_tag="ACV5NJ_002618"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HHY6486646.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACV5NJ_002619"
     CDS             complement(3284..4144)
                     /locus_tag="ACV5NJ_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486647.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DBKOIJ010000194         4234 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000194 DBKOIJ010000000
VERSION     DBKOIJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.198"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            34..471
                     /locus_tag="ACV5NJ_002620"
     CDS             34..471
                     /locus_tag="ACV5NJ_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486648.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            566..1450
                     /locus_tag="ACV5NJ_002621"
     CDS             566..1450
                     /locus_tag="ACV5NJ_002621"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HHY6486649.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1672..>4234
                     /locus_tag="ACV5NJ_002622"
     CDS             1672..>4234
                     /locus_tag="ACV5NJ_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HHY6486650.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1382 a          712 c          958 g         1182 t
ORIGIN      
        1 acgatattaa aaaatgcacg tagaagtaga taaatggagt ggttttgcaa atttctttta
       61 tttctagaag ttattcacgt atacggattt gtttttctca caaataaaaa ggcaggaagt
      121 acaatggatc atttgtatat aaaatatatt catcaatttt ccgttttggt attaggcaca
      181 ctaattatag cggggattgt tgcatttatt attcaaggtc gtgacatatt aacagtacat
      241 caagagtttt tcagttatgc atttggtatt cctctgatcg ctataaggtc tgataacaaa
      301 aagtggaaga aacgctatta cattctcttg ctatttttgt gtttttatct gccacctaag
      361 atttttactt tattaggtat acaatgccta tcattttctt ttatttccta tatcattgtt
      421 ttaattttcc tcagtttcat agtatgggta ttttatcttg tcaaaaaata aatgaatgtt
      481 tcatgaacag tacttcatca cagcctaatt tttttcttta tgctatcgtt atattaaaga
      541 aatcaatcag aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa accccagttc
      601 aggcggtgaa caagcaaagg aatttgagca attagcgata gcaaaattgg aatcggtgtt
      661 tgatgaagtg gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact ttactcgcga
      721 agcggctacg gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa cagtcaacga
      781 aggaatcagt ggaatcgctg aacaagaaca tcggccaaat ttcggatttt tcccattggg
      841 tacggtgaat gacttggctc gagcattagg gataccgcta gaacctgagg aagcgatcaa
      901 tcatttttcg attgaatcag tcaagccatt ggatattgga aaaataaatg acgactattt
      961 tatgaatgtc gtcgcaattg gctctatccc tgaagcaatc aatgatgtag atgcggagaa
     1021 gaagacgaaa tttggaaaac tagcttattt catgtcagga atcaaacagt tagcttccac
     1081 acaatcctac tcgtttcacg tagaagttga tggaaagaag gaagaaattg agagcagtat
     1141 cttgctgata ggactaacta actcagtagg cggttttgaa acattacttc caaatgcaaa
     1201 agtggatgat ggtaagctcc atttggttta tttaaaagat tcttctttga tcgatacgct
     1261 taaaacattg ccagacctat taaaaggagt agatgaatct acgaaaaatc tagcctatct
     1321 tacttgtgag caattagttg tgtcattagc tgatgaggaa gaattgacga cgaatgttga
     1381 tggagatgaa ggggataaat tacctgtgac aatcaaagtg ttaccctcac atttacaggt
     1441 ttattgttaa aatggaggtt gtgaccgaag agttgtcagg aggacatcgg ttgttcggaa
     1501 taagggactg tgacaagact tttgtcacag cctttttttg tacaaaaaag aaattttcac
     1561 ctaacagaaa aatttgccat aaaaatacat attttaaata tgaaagtaca attacaaata
     1621 aattaaaacg ttaaaataaa attgtcaaaa cgtcagagag gatggtggag aatgaatcaa
     1681 tatgtaaaaa agctgattgc gtcgattttg tttgtcttcc ctctcttgat ggtgacatcc
     1741 gtttttgccg atgtagagaa aacaagcgaa tcaacgcaat cagagaatca aagttttgac
     1801 aagactactt tttcagaaga atcaattact tctgaaacta cagaatccca aagtgaggcg
     1861 cctacttcta atacaaggca agaagaaaat aagacaagtg agacctcaga agtagctgaa
     1921 caaacgtctg atagcagtga agaggtacag gttagagttg tttcagcgga tgggtacagc
     1981 gaagctgcca caacagaaga actgacgcag cttctcgcag acgaatcagt agctaagatt
     2041 cgtttaactc agcctttgac tttagacagg gaattggaga tcaagcgaga tatcgttatc
     2101 gattttggcg gacttgctca taatttcggc acacatcaca tctatatcaa tgagacacct
     2161 cagtcgatcg agtttcagaa ttttaaagga acggcagctc atccaggtgg gttgatacca
     2221 aatatcgatg gaaatgccat tatcattgct tatatgtccg atttttgggg aaacagatat
     2281 cattttacgg gagaaataaa atttaccggg acacttgact tatatgatgg ttctaaatta
     2341 ggcctgattt acgcccctcg tgcaactgtc acattagatg gtgtttccgg tgtattggat
     2401 gtccaaccag tgaaagaagg gatgaatgcc gctccgggta aagcttattt ttgtagaagc
     2461 tatcagctaa atgtaatcaa tggtagtcag ctttatggac catatctagg aaaattctat
     2521 ggttttctag attcaggtga tgagacgggc agtggaaaaa cagcaccagg actccatatc
     2581 ttgagtggtt caaaggtctc tcttgactat gatcgggcag atggcgtcag cgatgaagga
     2641 gaggctgtcg atacgctacc cgggaatgtc acatttaaag tcagtggctc aggaagtgaa
     2701 ttttcggtaa atacaaagat caatattacc aacgacaaca atcgcggaat tatccaaatg
     2761 agaggctcag gatcaagagt cgctgtgtcg aatgatggga aaatcacaat caatacagcg
     2821 actactggcg gatttcgcct gcaaggagat tctagtcaaa tccatgtatc aagtggtgga
     2881 caaatctcgg tcaaaatggc tggagatggc gaccaacctg gaaacaatgg gatgcgtttt
     2941 gtagggaaag gactttcttt gatcgtagaa ggagccggat cagtcatcga tattgataag
     3001 caaagtggtc gttctagcgc agttcgtttt gagaacggta cgcaaaaact aacagtaact
     3061 aatggaggct cattgaaggt aagaaatgca ggggatggca aatcttatgt gaacagactc
     3121 aatcaaggaa accaagcgat ccaattttat gacgcttctg gttttctaga aagtccaggg
     3181 tcagctgatt ttacggttac tggagaaaaa tcaaatatcg atatacgcgc agattccgga
     3241 gctaccgtag atacaactgg agatattgat ttgaacttta tggctggcga aaaaacttat
     3301 ttagtcatgg tcgggaaaac tggggaagat aagactggca tcatcagtgg ggatgtgttg
     3361 aatgtagact tagaatctcc tacctatttc gatttccaaa acaaacggat cggaagcgag
     3421 actaatggca taggtggctg ggtatttcaa ggaaatggcg attcttcttt gaagatcgct
     3481 cattcagaaa ttgctttatg gcgaaaggca ggagtaacgt tcgataactt tgaaaaagat
     3541 ccggattatt tttctgatat gacagactta tcgatcaatg gaaccgactt aggtaatttt
     3601 gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc agacaggtgc cggaatgaaa
     3661 aattataatc gaatcagtgc caataaccag cttccgatga ttgaatcctt aagagtacca
     3721 acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc ccgaaggtgt cgaggcaaag
     3781 ccaagagatg cttggacagg cgaagtagaa gtgactttac gtattactta tgcagatacg
     3841 acgaaatcac ctgtagaaat tactgctctc acacaaggac aaacagatga aaatgaaggg
     3901 acaggatata atccgtgggg agaaggcgaa caaggcggtt tgtttgaggt tcaagtaccc
     3961 aacgaagaaa gactaacaac gggggatcaa gtgcaaatag ttgctgctaa aaaagtaaga
     4021 ggcggccaag aagtgacaga actggactct gccaaaacga cagtcgatgt gattcctcct
     4081 gagcctgcta aattaagtac cacattgatc aatgcagctt ctcgctcaat cacaggtaca
     4141 ggcgaagcag gagctttggt ttcattgaaa aaaaatcaag ctgttttgtc cgaaacaact
     4201 attgatgata caggtaaatt tgttctttct atcc
//
LOCUS       DBKOIJ010000195         4162 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000195 DBKOIJ010000000
VERSION     DBKOIJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.199"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..703
                     /locus_tag="ACV5NJ_002623"
     CDS             <1..703
                     /locus_tag="ACV5NJ_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6486651.1"
                     /translation="SLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYY
                     NDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKAS
                     NMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQ
                     ALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSK
                     DKVLENCELLKQKHK"
     gene            713..1507
                     /locus_tag="ACV5NJ_002624"
     CDS             713..1507
                     /locus_tag="ACV5NJ_002624"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6486652.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            1520..3286
                     /locus_tag="ACV5NJ_002625"
     CDS             1520..3286
                     /locus_tag="ACV5NJ_002625"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HHY6486653.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            3309..>4162
                     /locus_tag="ACV5NJ_002626"
     CDS             3309..>4162
                     /locus_tag="ACV5NJ_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HHY6486654.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNG"
BASE COUNT         1497 a          494 c          818 g         1353 t
ORIGIN      
        1 ttcattgtta aaatctttaa aagggttagt taaaaatagg gcattacttt ctataattgg
       61 tgcggctatt ttcttactcc ttgctcaatt attaattatg agtatgaata attatgtatt
      121 tcctgattac tataatgatg cacagggaat agttttaatg aattttatca atcctatttt
      181 ggttttaata gttgtttcgc ctttaacttt gtttttaagt aaaaaatatg gaaaaaaaga
      241 actggcatca gtaggaatgt tcttttcagc aatagtatat ttgatcttat ttttgataaa
      301 agctagtaat atgtatgtat tcttaattct tagttcggtt ggctatatgg gtttgggtgt
      361 gtttaatact gttatttggg caaatattac tgatgttatt gatgatcaag aagtaaagtc
      421 tgatcaaaga gaagatggga ccgtatatgc ggtatattct tttgctagaa aaataggtca
      481 agctctagct ggaggcgtcg gaggttggac tctttctatt ataggctatg actctttagc
      541 aaatgtacaa acgggagatg ttttagaaaa gttatataac gcttcaacat taattccatc
      601 aatttgtttt ctgattgtag gattaatttt atttttcctt tatccattat ctaaagataa
      661 agtcttagaa aattgcgagc ttttaaaaca aaaacataaa taggagagaa atatgaataa
      721 ttggatattt tgtattgatt cagatggctg tgttatggat acgatgactt ataaacacaa
      781 tctctttttc ggaccaattg ctgcagagat ttttgatgtt catcatagag aaaaattcct
      841 acaggattgg aataatatta atttatattc agagactcgt ggaataaaca gatttttagg
      901 tttagttaaa acattagaaa aaaatgatgt aacatcgatt aatcgattaa agcactgggt
      961 atctctaaca aaagaattgt ctaatccatc tttgaagagg gaaatagaat attctcagtc
     1021 agaggattta ttaaaagctt taaaatggtc ggaagaagtc aatcaacaga ttagtcaagc
     1081 aaaaggatta gataaaccct ttttaagtgc tagagatact ataaaagctc tgaaaaaata
     1141 tgggaagata attattgtaa gctcagctaa taaggaagct gttcaagaag aatgggaaag
     1201 acatgaatta ttatcattag ttgatgaact gtgttgtcaa gataaaggaa aaaaagaaga
     1261 tattattcga agtgtaattg aaaatggttg tgatttggat aagatattaa tgattggtga
     1321 ttctcctggt gatctagaag ctgcaaataa aaataaagtt ttcttttatc ctattttggt
     1381 caataaagaa aaagaatcat gggaaaactt aagaactgat gttttattta aatgtttatc
     1441 gggagattat gtagatattg aacaaaaata tatagatact ttttggaaaa atcttactaa
     1501 taagtagagg agaaagaaaa tgactataaa agtagattta cgaaaaaaac catataattt
     1561 agatgaagat gctatttctt ggattgaaga gacgattgat tctatgactt tggaagaaaa
     1621 aataggtcaa ttgtttgtta atatgggatc tagtcgtgat aaaaattact tgagtaatat
     1681 ggtcaatcag taccatattg gagctgtacg atataatcca ggaactgctg atgaggtata
     1741 tgatcaaaac tatatcttac aaacagaaag taaaattcct ctattaattg ctgctaatac
     1801 ggaagcaggt ggtaacggag cttgtacaga tggtactgaa gttggtgtag aagtaaaaat
     1861 tggtgcaaca aatgatgcga aatatgctta tgaattaggg agagtttctg ggattgaagc
     1921 atcggctatt ggatgtaact ggagttttgc tcctattgta gatattaata gaaattggag
     1981 aaatcctatc atttctagtc gatcttttgg acaaaaccct gataaagttt tagaacttag
     2041 tttagcttat atgaaaggta ttcaggagag tggaattttg cctgcagcta aacatttccc
     2101 tggtgacggt attgacgaac gagatcagca tctatctttt agtattaata gtttaggtac
     2161 ggaagaatgg gataattctt ttggaaaagt atactcaggc ttaatagaag ctggattacc
     2221 ttcaattatg gcaggtcata tccatatgcc agcttattca aaacatttta atcctgatct
     2281 gaaagatgaa gatttacttc ctgcaacttt atcaaaagaa ttaatcactg acttacttag
     2341 aggaaaactc ggatttaatg gattagtagt tactgatgct agtcatatgt taggaatgac
     2401 tggggcaatg aaacgatcag aattattgcc cacttctatt gcagctggat gtgatctatt
     2461 tctatttttt aatgatccag atgaagactt taattatatg atggaaggct atcgtaaagg
     2521 aattatcact gatgatcggt tattagatgc cttaaaaaga attttagggt taaaagctaa
     2581 attgggatta cacaaaaaag caaaagaaca aattttatta cctaagcaag aagctctgga
     2641 aaaaattggt ttaattgaaa ataaatcaat ttttaaagag gtggctgacc aagcaattac
     2701 attagttaaa gataaacaag atatttggcc tattgtacca gaaaaatata aaaggatttt
     2761 actagtagat gtacatgggg taaaaggtgg gtttggtgaa gtagttgctg gaaatagacc
     2821 tcaagccatt gatttattga aacagatttt gtcagcaaaa ggatttgaag ttgaaaaata
     2881 catttcacct gaagaagaat tgaaaggatt atcgcaagaa gaaaaaatta aaaatatagc
     2941 tggaatatat gctgctaaga gaccaattaa aaatttaact aaaaaatatg acttgatcat
     3001 taatatagct aatgttaatc caggcacagt tcaacgaatt gtctggccag caagtaaagg
     3061 aactccggat ataccgtttt acattcatga aattccaaca atttttgtat ctgtacagtg
     3121 tccgtttcat ttggctgatg tacctcaagt aaagacttat attaatactt atgatggaaa
     3181 acaagaaact ttagaaatat tggtggacaa actaatgggt ctttctgaat ttaagggagt
     3241 aagtccaata gatgcttatt gtggtttaaa ggatacattt atttaatatt tagataggag
     3301 tggtttatat gttgtatccc attatgacag agacgaggca agtaattgac ttaagcggtc
     3361 tctggagatt taaattagat acaaatgatg acttacagga agaacttgct ttagaaccaa
     3421 taaatgaaaa agagagttat ccgatttcag ttccctcttc ttataatgat ttatttgaaa
     3481 cagagaacat tagaaatcat gttggatggg tgtggtatga aaaagaagtg actcttccta
     3541 aaagattatt agaagaaaga ttagttcttc gttttggatc tgcaacacat gaagcaaaag
     3601 tttttttgaa tggaaagttt attactcaac acaaaggtgg gtttacacca tttgagtctg
     3661 aaattaatcg gtttgtaaaa gacgggaata atcgaattac agttgctgta aacaatatta
     3721 ttgacgatag tactttacct gtggggatta tgaaggaaac agtacaggaa aatggggata
     3781 taaaaaagga aaacttagtt aattttgatt tcttcaatta tgcaggtatt catagacctg
     3841 ttaagttata tacaactccg aaagactata taagtgatat tactattaat acaagaataa
     3901 ctgatttgga tgcaagtgta gaattcaata tcgatacaga atggaccgga aaaatcttaa
     3961 tagcgatata tgacgaagag gctcaacttg tagctgaagc tgaaggaaag aaaggaatga
     4021 ttcagcttaa gaatccaatt ttttgggaac ctctagacgc ttatttatat agtatgaaaa
     4081 ttagtttaat tgatgataat aaagtgatcg atacctatac agaagaattt ggtattcgca
     4141 gtgttcaggt taccaatggc aa
//
LOCUS       DBKOIJ010000196         4141 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000196 DBKOIJ010000000
VERSION     DBKOIJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.200"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..522
                     /locus_tag="ACV5NJ_002627"
     CDS             <1..522
                     /locus_tag="ACV5NJ_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HHY6486655.1"
                     /translation="LVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRK
                     GIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVVSWVLMQ
                     LGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGIWGIISVLVVASLSMIG
                     VLIGRKVLKKYKK"
     gene            632..2386
                     /locus_tag="ACV5NJ_002628"
     CDS             632..2386
                     /locus_tag="ACV5NJ_002628"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486656.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            2370..4112
                     /locus_tag="ACV5NJ_002629"
     CDS             2370..4112
                     /locus_tag="ACV5NJ_002629"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486657.1"
                     /translation="MKNFYKKKFALTDQGAEALTKASISSFFVYCINMVPAFIIMMLI
                     DELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQIADDLSNL
                     PLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLLVGNVKMGL
                     AVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINSFNLSKKVQ
                     DRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTGEVTILYVV
                     GYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLKSFDIELRD
                     VSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDEGCILIDGY
                     DIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARLANCEDFIE
                     KLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAENEKRIQES
                     LNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIYKNLIKKTK
                     LSEKFVYEKEAQSR"
BASE COUNT         1371 a          585 c          791 g         1394 t
ORIGIN      
        1 ctagtaattt actttttttc aataatggtt agtagtattg gtggggcgtt tggtcacagc
       61 atatccccag ggatttttgg attattatct ggaatcatat ttgtttatat tagctataac
      121 taccctagaa aaggaatttt tactatttac acaattgttt tattgttatt ttttacgtta
      181 atgggcgggg cgtacttacc ttggtttatc agttcgatta gtatggcgat cttagctgat
      241 attattctat ctgtatttgg atatgatcga gcaattcctc aagttgtgtc ttgggttcta
      301 atgcaactag gcagtgctgc cggacagtgg attcccatat ggttctttac agatcgtttt
      361 cgtcaagact ggatagatag aggacaatct gctgcaacaa tggatgcaat gatccactac
      421 gcggtgggta tatggggaat tatttcagtg ttagttgtag caagtttatc tatgattggt
      481 gtattgattg gaagaaaagt cttaaaaaaa tacaaaaaat agaacgtagt ttaccttcta
      541 tctgtttgta catctctaat ttacgctatt acggaaagca ttaacagttg taaacgaata
      601 agagaagaaa ttgaatgcgg gggaggaaaa aatgaaagta tacaaaaaat tatttagtta
      661 tgtccctaaa atgaaatact acggatggtt agccacttta ttttctgatt tttctgtcgt
      721 attaacagtc tatggatatt acagtattta caaatttctt cagtctctaa ttattgtagg
      781 agatgaatat ttagctaaga gttatgcggt tcaaactgtt gcttggttaa ctttaggagc
      841 tttattttat attttttctg gcttattttc tcatatcctt gggtttcgct tagagactaa
      901 tttgagaaaa aagggaatta gcggtttaac agaagcaagt tttcgttttt ttgatttaaa
      961 ttcttcggga tatattcgaa agacaattga tgacaacgcg gctaaaacac atatggcaat
     1021 tgctcacttg attccagata gtgctcaagc catagtaaca cctgttttat cagttgcctt
     1081 aggctttttc attaacttta aaattggatt ggttctaatc gccatgttag taattggtgt
     1141 attcttgtta aagaaaatga cagagaatac aaattttatt caaaaatatc aagaatcgtt
     1201 agatgtgtta agctctgaaa cggtagaata tatccgcgga attcaagtta taaagatatt
     1261 tggtgctaaa gtaacatcat ttaaagcctt gaataaagca attaacgatt atgctaagtt
     1321 tgcatataag tattctttga gtgggaaaac accttttgtt ctttttcaat ggctattttt
     1381 tggcatagtt tgcattttga tagttcctat aacctttttt ataacttctc ttaccactcc
     1441 caagatctta gctgttgact tgttgatgtt gtttttttta agtggaatta tttttgtttc
     1501 tttcatgaga ataatgtatg taagtatgca cttgtttgaa gcaagatatg cagtcgaaac
     1561 actagactcc ctttatttag aaatgaagca agaccaacta aaatatggga acgagacaca
     1621 gattaaaaat tgtgacattg catttgaaaa tgtcagcttt tcatacaaag aagagtgtgt
     1681 tttagaaaat ttcaacttag ttttagaaca aaaaagaatt tatgcattag taggaaaatc
     1741 aggaagcgga aagagtacga tagctaaact aatttcaggt ttctacaatg tggataaggg
     1801 acagattaaa attggtggta agcctttaaa tcactattca aaagacacaa ttattaacac
     1861 aattgctttt gtctttcaag atacgaaact attcaatcag agtatttacg ataacgtttc
     1921 tatagcaaat cctcaagcgc cagcaagtaa agtatttgac gcgctaagat tagccggttg
     1981 cgaatcgatt ttagaaaaat ttcccgaaag agagcatacc ttaattggta gtaaaggagt
     2041 ttatctgtca ggaggagaaa aacaacgtat agctattgcc agagcaattt taaaagatgc
     2101 aaagattgtt attatggatg aagccagtgc atctattgat ccagataatg agtatgaact
     2161 acaaaaggct tttcaatatt tgatgaaaga aaaaacagtt attatgatag ctcatcgatt
     2221 atccagcatt aaatctgtag atgaaattct tgtcttgtca gaaggaaaaa tagtagaacg
     2281 tggaaatcat aatgaattaa tggctaaaca tcgagtgtat aaacaattgg tcactaatta
     2341 tgagaatgca aatgattgga ggcttgacaa tgaaaaattt ttataaaaaa aaatttgctt
     2401 taactgatca gggcgcagaa gctttaacta aagcttctat ttctagtttt tttgtatact
     2461 gcattaatat ggtaccagca ttcattataa tgatgctgat agacgaatta gttttagaaa
     2521 atgcaaagcc ccgttggctt tattttgcag tatcgtttgt tactttgctt ttcatgtatt
     2581 ggttgttaga tagggagtat gaaaacttat ataatagtac ttataaagaa agtgcgcatt
     2641 taagagtgca aattgcagac gatttgtcaa atttaccatt atcctatttt tcaaaacata
     2701 atttatcaga tttatctcaa actatcatgt ctgacgttga aggtattgag catgcgatga
     2761 gtcatgcaat acctaaagcc ggcggtatgg ctctgttttt cccttttatt tcagtgatgc
     2821 ttttggttgg taatgtcaaa atgggattag ctgttatttt gccaacgtta tttagttttg
     2881 tcttaatctt gttatcaaag aaatcccaaa cgaaagccaa tactaaatat tacgatactt
     2941 tgagagaaaa ctcggaagaa tttcaagaaa ctattgaatt gcagcaagag attaatagct
     3001 ttaatctatc taaaaaagtt caagacagac ttttcaaaaa aatggaagag agtgaaagga
     3061 ttcatttaaa ggtagaatta agtacttttt cagtcatggc cttatcctct attttctcat
     3121 atgttagttt agcagtagtc attctagtag gtgttcactt actgttaacg ggtgaagtga
     3181 ctatactcta cgtcgttggt tacttactag ccgcaataaa aataaaggat tccttcgatt
     3241 ctatgaaaga agcagtgctt gaaatatttt atttagctcc taaaatacag cgaattagag
     3301 ctatgaagga aaccagtatc caagagggat cagatagtcc tctgaaatct tttgacattg
     3361 agttgcgaga tgtctctttc tcatatgata acaatacccc aattttagat catatctctt
     3421 ttacggctaa acaaggagaa gtgactgcgt tagtaggtgc tagtggttcc gggaaaacta
     3481 gtatcttgaa attagtgtct agactgtatg attatgatga aggctgtatt ttaattgatg
     3541 ggtatgatat taaacgtgta tctccagctt ctttgtttag caaaatcgct attgtttttc
     3601 aagaggtaac tttgtttaat acaagtattt tggaaaatat acgtattgga aatagtcaag
     3661 cgtcagatga ggaagtgaaa aaagcagctc gtttagctaa ctgcgaagac tttattgaaa
     3721 aactacctga cggttatcat acattggtcg gagaaaacgg aagttcactt tctggaggag
     3781 agcgacaacg cctatccata gcacgggctt ttttgaaaaa tgcgccaatt ttaattttag
     3841 atgaaattac tgccagttta gatgcggaaa atgaaaaacg tattcaggaa agtttaaatc
     3901 gtttaataca agataagaca gtactaatta tatcgcatcg cttgaagtct attgaaaagg
     3961 taaataaaat tgtagtgatg gatcaaggaa aactagttga tcaaggtact cataatgagt
     4021 tgtatcgaag gtcagaaata tacaaaaatc tcataaagaa aactaaacta tctgagaaat
     4081 tcgtctatga aaaagaagct caatcacgat gatgaaatag gctttaaatt aacctacaat
     4141 t
//
LOCUS       DBKOIJ010000197         4140 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000197 DBKOIJ010000000
VERSION     DBKOIJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.201"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            85..1386
                     /locus_tag="ACV5NJ_002630"
     CDS             85..1386
                     /locus_tag="ACV5NJ_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HHY6486658.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1413..1673
                     /locus_tag="ACV5NJ_002631"
     CDS             1413..1673
                     /locus_tag="ACV5NJ_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HHY6486659.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2672..3907)
                     /locus_tag="ACV5NJ_002632"
     CDS             complement(2672..3907)
                     /locus_tag="ACV5NJ_002632"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HHY6486660.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1179 a          668 c          864 g         1429 t
ORIGIN      
        1 tcttaattat ataattagtt atgaacattc tttgcgttgt catgcaatga atggttcata
       61 ttttattgaa aaggagtggt tttagtggaa aagctgatga aatgggtcga agagaaactt
      121 gtcccaccaa tggcaaaaat tggtacacag cgtcatttat tagcaattcg taatggggtt
      181 gtttcaacat tgtcattgat tttgattggg acatttttta tggtctttat caatttaccg
      241 tttcctggat ggaatgaatt tattgcccct tattcggcaa cgattgtttt accctttagg
      301 atgacgatgg gattgatggc tatttatgca acttttgtta tgggttcgga tctagctaaa
      361 tcatatggat tagatagtgt gacaggtggt atattatctt taggaacgtt ctttatgtta
      421 caagttcctg taaacgtttt aactcctgaa gaggcacctc ttggctgggt cttacctatg
      481 agttctttgg gagcatctgg gatgtttgcg gggatcttat ctatgatttt tgcagtggaa
      541 gtatatcgtt tctttaaaaa tcgaaatatt actatcaaaa tgccagaaca agtgccacct
      601 gctgttgccc gtagttttga ggcattgatt ccaggtgcag tcattttaac aattacttgg
      661 ctgattcgaa gcgtcgcggg ctttgatgta aacgctgcct tattatcgtt attcgagcct
      721 ttaaccaata ttttagggaa taatttgctt ggtgttttac taccaatgtt tttgatccat
      781 ttgttgtggt catttggtat ccatggcatg agtattgtag gggctgttgt tcgtccaatg
      841 tggctgatca tgttggatga gaatgcaaag gcattggcag atggtacagc tgccacccaa
      901 ttaccctata tcgctcctga acagttttat caatggacgg taacaattgg tggtgcagga
      961 gcaacgatcg tggtatctgt tttgtttata ttcttctgta aatcaaaatt cttgaaagag
     1021 gttggtcgtt tttctattat tcctggtatc ttcaatatca atgagcccat aatttttgga
     1081 gctcctatga tgttgaatcc ttacatgttc atcccgttca acttagttcc tttagttttg
     1141 actattatca gttattttgc agtcaaatta gaaatggtca atagatttac tgttttgcca
     1201 acatggacat taccagctcc aatcggcggt tatctttcag caggaaatga ctggcgtgtt
     1261 gttatcttga tcatgatcaa cacattgata gcatttatta tctattatcc atttgtaaaa
     1321 gcttatgata aaaagatgct gtcagatgaa caaagtcgtg gagaaaacga agtccagact
     1381 gtctaagttt ggtttttaga aggtgggaaa caatgtattg gctgatttta ttttttgttt
     1441 ttatcttttt attaacggct agtcacctta tattgaatat gctagctaca tatcatattc
     1501 aaatcaatcg atggatttgg gcactggcat catttttgat cgtgattctt ccaaagatca
     1561 ttgtaccgca tatgaatgtt ttgttttcat gggggacata tgtactttgt ggaatttttg
     1621 ctatcaattt tatgatcgag cagcatagat ggtttgtcac atcgaagctt taaatgttct
     1681 aaaagtggtt gtaacagaag tggttagctt caagaaataa gaagaaattt acgaaaattg
     1741 cttcttaaat ttttgtgaaa tgttggctta tttttcgttt ggttgaactt attaccgcag
     1801 atgcaagctt ttgaccaccg tttattcgga atatgggact gtgacaaggc ttttgtcaca
     1861 gccctttttt acataaagat tattattgtt ttaactgttt tggcagaaag aaggaggaaa
     1921 aaatggaaat tttatcagca atggatttag aattagtaga tctactacca aagtgatcaa
     1981 gtcaaatata gatttgggag cttacaacca tatagtaggg gctgcagtat ggtgtatgaa
     2041 tggtaatatg tatgatgtga gtgtttattc aaatcatgaa acgtgtatag aaataattgt
     2101 gctgggaata gcaatcagtc atggtgaacg tgacttttag tgtatcgttg ctgacgacat
     2161 agaacattct gacatgatct actcaatatg tggggattgc tggcagttta tataggagta
     2221 tgcatcggat tgcgagatca ttgtctcaac agaagaggga ggttgattat caaagataaa
     2281 gtggtaaatc ctacatttga tttagtaatt gttgagggga ctagcggcac aaatctctac
     2341 aaagcgatgc ttctagatct atatgacaca ttttaagaaa atgtatttgc ctaattatta
     2401 tggtctttct tttgaagaaa cgttgagatg atccaaccag agaaaggtgt caaagaattt
     2461 gactaagaat aaatggcaga cgctacctat gctcatttgg atagcgtcag atcttttatt
     2521 ttaaggatga ttttgtgttt tttagacacc gtaatttaag taactctaac ttttcaatcc
     2581 actatttact ttttttgtat tttcactgtc tgggtctcga atagcgaaat tactgatgag
     2641 tctctattaa aaaagctttt tctacttgaa actatattgg gtggttgctc aggaaggttt
     2701 cgatttcttg aattgcttgt tgtggaccat ttattggaaa aacggcttgc agcgtgcgtg
     2761 cctgctgacg aatcttgggt gaagaaactt cgcgcagagc aatctgcagt tttctttcag
     2821 tcactggttt tgtgagggcg attgcgtttc cgtattttac acaatagtca atattacagc
     2881 tttgctcaaa ttgcatcccc acaccgataa acggtttgcc ggattcacag gcagtttgga
     2941 cggtcccttc cccgccgtgg ataaaagaaa aatcaatgaa ttcatgcaaa agatgactag
     3001 acagatattc tgttattaaa agatcttctg agtcagattg aatgtcatca agcagatagt
     3061 caataggtgc aataaccgct agatccggtt gtttttgtaa gaagaacaaa attttgcgta
     3121 acatacgcgg attgcctgaa ctacccattg cgaaatagat aatacgtttt ctttcttgtt
     3181 gctttcgctg tataaaagtt agcacttctt caggcaagga agtactgagc ttagcaaaaa
     3241 ttggaccaac gattcgataa ttttccggca gctgttcttc ttccaaaaat agtttaggcg
     3301 ttgtgatgag atttagatca gcatccatca tttgtaaact tgtttttggt aaaggaagca
     3361 tttctttttg tgcagcgtgt ttgaaggcat tggggatcca tcgggtattt actatacgct
     3421 tttttgtcca gtgccaaagg ggattgctta aagggccgaa gaaacgtaac tcgtggggca
     3481 tcagcgcatg ctctgattga aaaaaaggtt cactcaaagc atagggttta acatatacta
     3541 aaggaatctt tttattgcgg gccgataaaa aaattgtcac attcgaacct gtcacaatca
     3601 gtcgaggctc aaacttagca atcagactct gttcactagt gatacgagag gcaaccattt
     3661 ctttggtaaa agggttttta agtgtacgca actggtcaaa ttttatgatt tgttcttctt
     3721 gttttttcgt aaggatcggc gctagtaaat gatactcaaa gccactttct gtgatcaaag
     3781 gagcaaaagt tttagaaaag ccaaagaata cacagtaata acggtctttc atagctttgg
     3841 caacctcaat cattcgtgtt gtctcagcta aattgaatac tgcaggagca aaaagaattg
     3901 tcttcatgaa atcctcctta ttggaatgag tctatccctt cgtttcttac tagtatacta
     3961 aaaaacacag cgttcgagta gcctatgtat attttgaata ttaaactgtc tctcgctgtt
     4021 ttcacaactt ctaaatttaa attttgtaat caagttgaaa ctccaatttt caaaaaaaca
     4081 cgcaacaatt ttttgatcga tttatatgtt gtgccagata aatacgagaa gttgtgtcat
//
LOCUS       DBKOIJ010000198         4040 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000198 DBKOIJ010000000
VERSION     DBKOIJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.202"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            178..774
                     /locus_tag="ACV5NJ_002633"
     CDS             178..774
                     /locus_tag="ACV5NJ_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HHY6486661.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="ACV5NJ_002634"
     CDS             898..2697
                     /locus_tag="ACV5NJ_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HHY6486662.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="ACV5NJ_002635"
     CDS             complement(2975..3187)
                     /locus_tag="ACV5NJ_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486663.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="ACV5NJ_002636"
     CDS             3649..3798
                     /locus_tag="ACV5NJ_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486664.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1189 a          689 c          711 g         1451 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg agatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata
//
LOCUS       DBKOIJ010000199         3984 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000199 DBKOIJ010000000
VERSION     DBKOIJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.204"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(93..1598)
                     /locus_tag="ACV5NJ_002637"
     CDS             complement(93..1598)
                     /locus_tag="ACV5NJ_002637"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HHY6486665.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1595..2281)
                     /locus_tag="ACV5NJ_002638"
     CDS             complement(1595..2281)
                     /locus_tag="ACV5NJ_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHY6486666.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2474..3895)
                     /gene="gndA"
                     /locus_tag="ACV5NJ_002639"
     CDS             complement(2474..3895)
                     /gene="gndA"
                     /locus_tag="ACV5NJ_002639"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HHY6486667.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1097 a          848 c          684 g         1355 t
ORIGIN      
        1 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
       61 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      121 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      181 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      241 gctcttgcat tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      301 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      361 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      421 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      481 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      541 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      601 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      661 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      721 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      781 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      841 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      901 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
      961 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1021 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1081 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1141 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1201 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1261 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1321 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1381 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1441 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1501 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1561 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1621 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1681 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1741 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1801 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1861 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1921 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     1981 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2041 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2101 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2161 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2221 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2281 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2341 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2401 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2461 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2521 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2581 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2641 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2701 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2761 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2821 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2881 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     2941 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3001 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3061 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3121 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3181 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3241 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3301 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3361 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3421 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3481 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3541 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3601 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3661 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3721 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3781 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3841 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3901 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     3961 tgcaaactaa agaacaacat attt
//
LOCUS       DBKOIJ010000200         3968 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000200 DBKOIJ010000000
VERSION     DBKOIJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.205"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(74..616)
                     /locus_tag="ACV5NJ_002640"
     CDS             complement(74..616)
                     /locus_tag="ACV5NJ_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HHY6486668.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            751..1140
                     /locus_tag="ACV5NJ_002641"
     CDS             751..1140
                     /locus_tag="ACV5NJ_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486669.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1144..1908
                     /locus_tag="ACV5NJ_002642"
     CDS             1144..1908
                     /locus_tag="ACV5NJ_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHY6486670.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2037..2984
                     /locus_tag="ACV5NJ_002643"
     CDS             2037..2984
                     /locus_tag="ACV5NJ_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6486671.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3114..3854
                     /locus_tag="ACV5NJ_002644"
     CDS             3114..3854
                     /locus_tag="ACV5NJ_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486672.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1315 a          676 c          805 g         1172 t
ORIGIN      
        1 tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt
       61 ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg
      121 aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc
      181 gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt
      241 ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca
      301 aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg
      361 ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga
      421 caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa
      481 tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca
      541 tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca
      601 tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa
      661 tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat
      721 agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc
      781 agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca
      841 attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt
      901 tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat
      961 ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc
     1021 ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag
     1081 acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa
     1141 caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt
     1201 ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca
     1261 agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc
     1321 ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat
     1381 acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta
     1441 gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg
     1501 acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt
     1561 tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat
     1621 gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg
     1681 cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa
     1741 atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa
     1801 gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa
     1861 gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa
     1921 atcactcatg acgatagagg attttccgtt ttttattttg ggctataata aactctgtta
     1981 ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc
     2041 ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca
     2101 tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg
     2161 aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca
     2221 tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga
     2281 tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg
     2341 ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg
     2401 attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg
     2461 attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa
     2521 gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa
     2581 gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt
     2641 ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag
     2701 ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta
     2761 taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt
     2821 tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg
     2881 aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc
     2941 gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata
     3001 tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca
     3061 atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag
     3121 aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa
     3181 ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc
     3241 agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc
     3301 gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa
     3361 agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag
     3421 gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag
     3481 agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt
     3541 ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg
     3601 aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag
     3661 aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa
     3721 caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta
     3781 tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata
     3841 gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg
     3901 cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg
     3961 agtttaag
//
LOCUS       DBKOIJ010000201         3949 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000201 DBKOIJ010000000
VERSION     DBKOIJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.206"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            26..2695
                     /locus_tag="ACV5NJ_002645"
     CDS             26..2695
                     /locus_tag="ACV5NJ_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HHY6486673.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(3150..3896)
                     /gene="truA"
                     /locus_tag="ACV5NJ_002646"
     CDS             complement(3150..3896)
                     /gene="truA"
                     /locus_tag="ACV5NJ_002646"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HHY6486674.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         1111 a          790 c          753 g         1295 t
ORIGIN      
        1 catagaaatt tggtggaaaa taaatatgaa aaaaaattta aagtcattct ttagagaaaa
       61 cggacgctac gcagcagcta gtttcctgat tccgtttttg attttagcac ttgtttatct
      121 aagtatcgga atctatcctg gaagcagcag aagtattttg gcaagtgatg ccttttcaca
      181 gttttctaat tttcatgcca gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta
      241 tacatggaat gcttctctag gattgaatta tttatcttta atttcttact atctaggcgg
      301 gatcttcacg ccactggtgt tcttttttcc aaaccagatg atgccagatg cactgtatgt
      361 tctgactttg cttaaggtcg gctgtgccgg attggctttt tggtttcttg cacgtacgtt
      421 caagattcct cgctgggggc atgttgcact tagcgtcagt tacgcattga tctcatttat
      481 cactgcacat tcggagatca tcatgtggct ggatgcattt ttttatcttc cattagtgat
      541 tttggggatt catcgtgtta tggatcttaa acggccgaaa cttttattta tcagttattt
      601 actcttgttt ttatccagtt tttatatggg ttttatgatt ggcgtatttt ctttcttata
      661 ttttatcgcc cgtctattga caaactggtc agaatataaa aaaacgattg ttccttatgg
      721 tatcacttct ttactggcag gcggcgcttc aatgatcatt gtattgccag cagtgctgga
      781 tcttcggtca aatggcgagg cactgagtca gatcactacg ttgaaaacag aagcgacttc
      841 ttatctggat ctaatcatga aaaatatgat tggtgtttat gacacgacaa aatacggatc
      901 gattccgttc gtttacgcag gtctgctacc attagtcctc tgtctgtttt attttgccag
      961 tcggaaagta ccgttgaaaa ataaattttt gtacggcagt cttttcgcca tacttatcgc
     1021 aagtttttat cttgttccat tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt
     1081 tttattccgt tatgcctatc tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg
     1141 ggaaaagata gaacaagacg atcgtgggct gttgttagga atcattttgg tcgtagctgc
     1201 tctctttgcg gtggcatggg gcatcaaacc tgaaggaagc tacacctatg tgacgatgac
     1261 ttcctttgta ttgacagtgg ttttcttagg gatttatgct tttgctgtgg gattttatca
     1321 gcataaaaaa ctttcattca atcatctgag tatattgtta ttattgatca tgtctgctga
     1381 agcactggtc aatacgaaca gtatgatcca tggcattctt gatgattgga attatgcttc
     1441 ccgcagttta tatactgatc cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa
     1501 agattccgac tcgttttacc gcttggaaaa tctggatcca gtatctgcaa acgattcgat
     1561 caactatggt tacagtggta tcagtttgtt ttcttctata agaaatcgta attcttcctc
     1621 ttacctggat aaattaggtt tccgttcaag agggacaaat ttgaatatcc gttataacaa
     1681 taatactttg ctgatggatg ggtttacagg aatcaaatat aacatttcta aaaacgaaag
     1741 cacgttcagt aaatatggct tccgccaagc tggtcaaagc ggcgactata cactgtatca
     1801 gaatgtcaat gcattgccac tagcatttac tgcaccgttg tccgttaatg atgttgagca
     1861 gccagtgaca gataatttga ccagtcagac aaacttgatc aatacattgt ctggtctaaa
     1921 tgaacagtat tttacattct atacacctac tttgaagaat cagttgaata cgacgatcac
     1981 aaagacaacg actggcgtca cttactcaga aacagcagat aaccagccaa aagagctcac
     2041 ttgggaagtc catgtgcctg caaatacaca agcttactta agtttgttcc caaccaattt
     2101 tgcgcaatta gaaagttcta gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca
     2161 aatcaacatt tctggtcaat actatgatct tggctattat cctcaagaga caacagtgac
     2221 atttactgca agtttttatg ggacgaaaga aataagtttc atggagccta aagtcgttgg
     2281 tctagatgtt gtagcctatc aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat
     2341 ggaagccact ggcagaaaag cttctgggac tgtaaatacc gctgaagatt ccatgttggt
     2401 gacaactatt ccttatgaca aaggatggaa agctaaaatt gatgggaaac cagtcaaggt
     2461 cagcaatttc aaagacgcat tcttgatgat aaaagttcca gcaggtaaac atactgtcac
     2521 tttcagttac ttgcctgaag gatttacgat aggtgcaatc ctttttgtcc tatgtattgc
     2581 attgtttatt ctttatgtct ggtaccttga ccgcaatcgc cccttttatg gcacgattga
     2641 tccagcaata gaagaacaac cacgaaacac acacagaaga agacgtagaa aataaagaaa
     2701 cgatggaacc tgttcagact gtccgcacca agggcaatca aaacaacgtt ccatcgtttt
     2761 ttggttacag aaaaaatagg taacaaaaag atgattcact tatcaacgcc tcgtccacaa
     2821 gtcacgatat tcatcaaaga tgagaagctg ttttttgaaa ttgctctttg tgactcagag
     2881 ttaaatgaca ggctcacgac ctctttctta aacggtgttc aatcagctga ccttcggtat
     2941 tagctcaaca aacggcaaaa tatgaggagc tattttcccg tttgtttctc taatactcag
     3001 gtcaaaacgc tgatttcacg acctcttcct taaacggtgt tccagaagta gcttcttcga
     3061 aaataagccg aaattttgca aaattttgag aagtaatttt cgtaaatacc ttcttatttc
     3121 ttgaagctga ccacttctat cataacctct tattcatatt tcacttcata caaataaagt
     3181 ccttcagggt gtgctgtagg tcctgctgca ttacgatctt ttttcgcaat aatatctggg
     3241 atactatctt caggcaatcg gccattgcca attttcaaaa gcgttcctac taagatccgt
     3301 atcatcttat acaagaagcc atttccccta aatgtaaaca acaattcgtc ttttgtttca
     3361 tttacctcca tcttagcctc gtagatcgta cgtattttat cctctatcga gcttcctgat
     3421 gcacaaaaag aggtgaaatc gtgagtccct aataaatcag gaagcgcccg ctgaattttt
     3481 tccaaatcaa gtgggtaagg aaaataactt gcataatggc gtcgaaaagg actgcgtggt
     3541 ttccctatat ccactcgaaa ttgatacgtc ttttctttga ccaaatatct tgcgtggaag
     3601 tcttctgaaa caatttctac ttgtcttaca gcaatatcct ctggtgactg ggtatctagc
     3661 gcgaaacgca ttcgttctaa cggacgctcc tctggataat caaaatggat tacttgcccg
     3721 atcgcatgta ctccggcatc tgtccttccc gaaccaaaga ccgtgatttc ttttccattt
     3781 gccatttttt gtaatgtttt ttctacttct tcttgaacag tacgcccatt tggctgtttt
     3841 tggaatccat taaaatttgt tccgtcgtac gcaatgattg ctttgtaacg taccatgttt
     3901 ttctctcctt tgttcctaaa aggttatctg gaataatgat ttttctata
//
LOCUS       DBKOIJ010000202         3934 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000202 DBKOIJ010000000
VERSION     DBKOIJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.207"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            346..819
                     /locus_tag="ACV5NJ_002647"
     CDS             346..819
                     /locus_tag="ACV5NJ_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HHY6486675.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            816..2948
                     /gene="feoB"
                     /locus_tag="ACV5NJ_002648"
     CDS             816..2948
                     /gene="feoB"
                     /locus_tag="ACV5NJ_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HHY6486676.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2950..3084
                     /locus_tag="ACV5NJ_002649"
     CDS             2950..3084
                     /locus_tag="ACV5NJ_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HHY6486677.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3178..3822
                     /locus_tag="ACV5NJ_002650"
     CDS             3178..3822
                     /locus_tag="ACV5NJ_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6486678.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
BASE COUNT         1240 a          694 c          863 g         1137 t
ORIGIN      
        1 ttagtaaaaa agtcaaacaa caaacactac atattgtgtt gtggaaactg tggaatacta
       61 agttttgtgt ttttgaaaaa agagctgaaa acaagtatcg tgtgggaaaa tagcaaattt
      121 aatagagaaa aaattgtgga aaactatgaa gaaatatttt tttctcattt ttatctatca
      181 atactaaaga ttcgctcata atatcacaag tataattgac attctcaatt accgagagta
      241 ttctatgtga gaatgattct catttatttg cgtaacacgt aagaaaatta gaacatataa
      301 tgaatgattt taatacatga tttatttcac aagagaggaa tcaatatgca gaccttagcg
      361 caggcaaagc agaatttcgt ttatcaagtc gaacgaatcg aaacagaaga aaaaacaaaa
      421 cgccatttgc aaaatatcgg gatcgttgcc ggaagtaaaa tcgtcgtcgt caatttatca
      481 ggcaacaatg ccattttact ggtaaaaagt catcgagtgg cagtaagtcg ggaaatacta
      541 ggtcaaatct ttgtcaataa aaagactgct tcctctgaaa gctggacttc tcttgatcaa
      601 ttaaaagcag gagagactgg gatcgtcgta gcaatccacg gacaaggcgc agtgaagcga
      661 cggttaatgg atatgggatt gacgcgaaat gtaaaaatag ttatccgaaa actagcccct
      721 ctgggagatc caatcgaact gacagtgcga gggtatgaac tgacgttacg aaagtcagag
      781 gccgaactaa tcttgataca aagggaggaa aaagaatgaa tatcgcgtta gtcggaaatc
      841 caaacagcgg gaaaactagc ttgttcaatc tcttgactgg ttcgaatcaa tatgtaggga
      901 attggccggg cgtaacagta gaaaaaaaag aaggatcgtt gaaaaaaaat ccgaacattc
      961 atattcagga cctacccggc atctattctt tgtctcctta tacaccagaa gaagtgattg
     1021 caagagatta tttggttcag gaacagcctg aactgatcat caacattatt gatgctacca
     1081 atttagaacg aaatctttat ttaactacgc agttattgga tttagacatt cctatgatca
     1141 ttggtctgaa tatgatggat gtcatcaaaa aagaaggaaa aaaaatcaat ctagaaaagc
     1201 tttcctatgg tcttggtgta ccagttttac caatcagcgt attgaaaaag caaggaatcg
     1261 aaaaactaat cgaaaagatt caggagtctg caaaaaagcg gccttctatt cctgttccct
     1321 gtacatatga tccacgttta gaagcagctt tacatgaaat tgaagatgta ctaggagaac
     1381 aaagcggcaa gaaacgctgg tatgcaatga aatattttga acgagatcag aaggtaagag
     1441 aggattacga gatagcagtt tctcagcaaa aagagatcga gcaactgatc cagttaacag
     1501 agaaactatt ggacgacgat agtgagacga ttctggtcaa tgaacgttat gaatttatta
     1561 cccaactatg tacactctgt gttgtgtcaa atgattcttt ccaattatcg atgagtgaca
     1621 aaatcgatca gattgcaacg aatcgttggt tagccttgcc gatcttcgca tttgtgatgt
     1681 ggctgatcta ttatctggct attcaaacag taggaacaat ggggacagat tggatcaatg
     1741 acacattgtt tggtacatgg ttaccagaac atgttggcag attgttagct gaatggcaag
     1801 tagcgggatg gatgcaggag ctgatcttga acggcatcat cgcaggcgtg ggagcagtgt
     1861 taggatttct accgcaattg attatattgt ttttatgctt gagctttctg gaagactgcg
     1921 gatatatggc gaggatcgct tttgtcatgg atcgactttt caggaaattc ggtctttcag
     1981 gaaaatcctt cattcctatg ctgattgcta caggatgtgg agtgccaggc gtcatggcca
     2041 gtcgcacgat tgaaaacgag caagatcgtc gtttgacgat catggtcaca accttcatgc
     2101 cttgttctgc aaagttgccg attattgcct tagtagcagg ggcctttttt cctcatcaat
     2161 cttgggtagc tccttctgcc tattttcttg gtatgactgc aattattttc tctggaatca
     2221 gcttgaaaaa aacacgtttg tttgctggag atacagctcc ttttatcatg gaattgcccg
     2281 catatcattt tcctcaatgg tcagcggtcc taagacaaac ctatgaccgc agcaagtctt
     2341 ttgttaaaaa agcaggaacg attatctttg tttctagtat cgtgatctgg ttcagctctt
     2401 cttataattt ttatgctcaa cctgtttctg aagaccaaag tatcttagcc tttttcgggc
     2461 gaatcctggc agttgttttt cagccgcttg gttggggaaa ctggcaagga acagtcgcag
     2521 cgattacagg attggtcgct aaagaaaata ttattggcac atttggtatt ttgtttggtc
     2581 atgcggaagt atccgaaaat ggcagagaaa tctggcaagt gctacaacac acatatactc
     2641 ccgcagcagc ttattcattg ttagccttca atttactttg tgcgccttgt tttgcggcaa
     2701 ttggggctat ccatcgggaa atgaatgcca agaaatggac ttggatcgcc ataggctatc
     2761 aatgcggttt agcttattta gttagttttg tgatttatca gttgggacac ttattggagg
     2821 gtggacaggt agcgttaggg acgtatcttg ctattttact gatgatagga ctcgtttatg
     2881 ttttgttccg tcagccaaaa tccaagaacc agttttatac tatattgtca ttagaaggag
     2941 aggaatagga tggcgacatt cttattaagt gtgttaatct ttggaagtgc aggcgcaatt
     3001 gtctatactc gtttaaaatc cggtaagaat tgtgatgact gtcaaacagc ttgcccagta
     3061 aaaaaggaac agatcaagcg atgatcagtc gttgcaagag gctattctcc ccttttttgc
     3121 ttattttgct agagcatagt aagatgaggg aaagtaaaaa aagaaagagg aaagaaaatg
     3181 tctggaaaga aaaaaatcgt ccgcccgcgc taccagcaga ttgctgttga tttagccgaa
     3241 cgtatcgttg aaaaccgata taaagtgggt gagaagatcc atgcccgctc aacacttgcc
     3301 agtacgtaca atgcttctcc tgagactacc cgcaaggcta tcaatgtgct ggtagatctt
     3361 ggaatcatgg aagtacggca tggaagtggg gcatttgttg cttcgaagga aaaagcaaaa
     3421 gatttcctat tgcaatatca agatgtacaa tccatccaag atacaaaagc agaaatcatg
     3481 gcaagtgtgg agaagcaaca agatgagctg aatcattttt ctcaattatt ggatcagctg
     3541 gtcaatcaaa cgaagcgaat acataagatg aatcctttac ttccgtttga attatcttta
     3601 tcagaaaaag caatgcatct ggaccgttca attagcgagt tgaatatctg gcaggcgact
     3661 ggcgccacag ttatcgcaat ttctcataat gaagaactgc ttgtttcacc tggcccctat
     3721 gctaaattta cggcagggga catcgttcat tttattggaa atgaatatac actacagcgt
     3781 atgacaaact ttttttaccc ggagcatcag cttgcagaat gattgtgtga aatattaaaa
     3841 aaatattaac ttggctaaac cttttattta caaagattta gccaagtttt tttcagtttg
     3901 gacaagcaaa tttctttcat ttttgacaaa gtga
//
LOCUS       DBKOIJ010000203         3864 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000203 DBKOIJ010000000
VERSION     DBKOIJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.208"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..371)
                     /locus_tag="ACV5NJ_002651"
     CDS             complement(<1..371)
                     /locus_tag="ACV5NJ_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486679.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRIN"
     gene            complement(382..1050)
                     /locus_tag="ACV5NJ_002652"
     CDS             complement(382..1050)
                     /locus_tag="ACV5NJ_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HHY6486680.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1181..1765)
                     /locus_tag="ACV5NJ_002653"
     CDS             complement(1181..1765)
                     /locus_tag="ACV5NJ_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6486681.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2096..2329)
                     /locus_tag="ACV5NJ_002654"
     CDS             complement(2096..2329)
                     /locus_tag="ACV5NJ_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HHY6486682.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2344..3294)
                     /locus_tag="ACV5NJ_002655"
     CDS             complement(2344..3294)
                     /locus_tag="ACV5NJ_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HHY6486683.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3291..>3864)
                     /locus_tag="ACV5NJ_002656"
     CDS             complement(3291..>3864)
                     /locus_tag="ACV5NJ_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HHY6486684.1"
                     /translation="KGDTAIGITFSSFLALGVILIGVANSSTDLFHILFGNILAVQDI
                     DKWITIGVSVLVLVTVVLFFKELLITSFDPLMAKAMGMNVSFYHYLLMILLTLVAVTA
                     MQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGACSSLLGLFIGYSFNIAAGS
                     SIVLTAAFLFVISFLISPKQKFLRKKERKL"
BASE COUNT         1297 a          694 c          498 g         1375 t
ORIGIN      
        1 tcattgattc ttttatccat atgctctttt gtaaatccaa agttttccat cgaaaatact
       61 aactcactcg ttgtatttgt tgtaaagaat tgtcctctag gattttgact tacgtagcca
      121 atattttttg atatactttc ttgtgttaag gattgtagcg agttattttc aaataaaatt
      181 tccccaacca attttccgtc ataaagtctt ggaataagtc cattcattaa ctttaatagt
      241 gtggatttcc cactcccaga agaaccagta atcaatgtaa gactgccttt ttttattgaa
      301 aaattaaggt caacaagcgc ttgttcctta acatccttat atgaaaaaga aacattgtta
      361 aattcaaaca attctttcac ctcaaatcaa aatacttaag aaaaaaatag taacggaaaa
      421 caggatgata tagtctaata agctcatgtt tgttttatag taagaacttc ttttcacagg
      481 agattctatc ccctttgtta ttgcattagc ggtcatttct tcagcaatca tagttgcacg
      541 tataagaacg ggaactaaca gaaattccaa atattgcaga ggctgaaaaa aagacaagcg
      601 aatgtttctc atttttaaag attctttaat cattcttatt tcagaacgat aagtcggaaa
      661 gaatctgatc actactgtaa tacttaacat tatttttttc ggtaaatgta ttttttctaa
      721 agaggataat aattcattgc tagtatagac actaaccaac aaccatgaga tcattacgac
      781 cggcaacatt cgaaatacaa tatataagga tgtcccaaaa aatatagtaa aaaactctat
      841 cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca agatgaaata
      901 aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa tgaaacaccc
      961 cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa tatttaaagt
     1021 aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata agatcgcact
     1081 gttatcttaa gctgacaaaa atgcaccgtt attcagataa cagtgcgatc ttatcaaaaa
     1141 gcataactga gaacaattct caatgcaagc ttttttttaa ttaactcagt aattcaatcc
     1201 acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt tcttttgaat
     1261 agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag aagtggattt
     1321 cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac ttatctagac
     1381 aacagactgt cttttttgtc acaaatgatg taattttttc aacaatatct tgatatttgg
     1441 ttccagctga ttggaataaa accaaatcga atatacgttt attctcaaaa atataattaa
     1501 taaatggatc taattcattg aagttctctc tccaactttt ctctatatct atattttcta
     1561 agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca ttaatatgcg
     1621 gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa aaagcacccg
     1681 tagttacgcc tgcggtcaag cacagttgcc ttatcttagt ttgctgaaat ccttcttttt
     1741 caaaatggct tagtccactt tctattaatt tttggtgagt tgtaatataa tctctggttt
     1801 ctatgggtac ttcctcctaa taagatgaat taactatatt atcttacaca ggacttgaag
     1861 tttcaatcgt taacgtctat tactgaactc attccttaga gctaaactaa tgagaaagag
     1921 caaaataact gatacaaaaa gcaatcctac aagaattaat agactcatct aaatcattca
     1981 ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat tagggtaagt
     2041 cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa gtcttttact
     2101 taaaaccttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat tgaaatccta
     2161 tatctaaata gtcctcatat ctaagtttag ccagcatctc acttacagat gctggactaa
     2221 cacttagaac ttttgctaaa tcattatttt ttaatttctt ttttcttttt gataattcct
     2281 gaattgctag taaataaatt agtatagtag tcgttttaat ttgtaccata cctaccctcc
     2341 aaattatttc gaaaggcctt cagcaatttt atccaggttc catttcatca tcgcatagta
     2401 actatcacca tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg aatagatagg
     2461 aatattggta tcttttgata ctgttttcat tggtctatcg tccacactgc tttctacgaa
     2521 taaggaggga acttttgttg tgcgtaattt ttcaactaag tgttttattt gatctggtgt
     2581 tccttcttct tcagtgttga tttcccaaat gtaagcagaa ggcacattat acgctttcga
     2641 gaaatattta aagcatcctt cacttgtaac aatcattttt ttatcattcg gaattgaagc
     2701 aaatttagat ttagcttctt tgtctagaga atccaatttt tcaatatact tatctagatt
     2761 ttctttatag aattttttat tatcaggatc tttttccgct aactgttttt caatattttt
     2821 agcgtaaata ataccgtttt ctaaatttaa ccaagcatgg ggatcttcct tccctttctc
     2881 actctgaccc tctaagtaaa taacatctat gccatcactt gctgcaaaat agtctttgtt
     2941 ttcctctttg ttcgcatttt ttactaattt tgtaaaccaa gcatttcctc cagtttccaa
     3001 gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat cttcaggcaa
     3061 aggttcatat tcgtggggat ctttcccaat aggtacgata ctgtgtaaat caattttatc
     3121 ttttgctata ttttcagtaa tatctgctaa aatcgaatta gtaactacta cctttaattt
     3181 ttcgttcgaa gccactgtat ctttgtcttt actatttgtt gaacatgccc cgaatataaa
     3241 aagagcaatc aaaatcccca gacttcccag tactgctttc cattttctca tcataacttt
     3301 ctctccttct ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat tacaaataag
     3361 aatgccgctg ttaaaacaat acttgatcct gctgctatgt taaaactata accaatgaac
     3421 aaacctagca atgatgagca cgctccgact gtcgccgaaa taaatatcat agtttttaag
     3481 ctgtttgcat ataaatatgc tgtagcagct ggtgtaatta acattgcaac aattaatata
     3541 gttcccacac tttgcatagc tgttactgct actaaagtta atagaatcat taatagataa
     3601 tgataaaaac tgacattcat ccccattgct tttgccatta atggatcaaa agaagtgatt
     3661 aataattctt taaaaaatag aacaactgta accaacacta aaacagaaac gccaattgta
     3721 atccacttat caatatcttg tacagctaaa atatttccaa ataagatatg aaataaatcc
     3781 gttgagctat ttgcaactcc tataaggata actcctaatg ctaaaaaaga actgaaagta
     3841 ataccaattg ctgtatctcc tttt
//
LOCUS       DBKOIJ010000204         3863 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000204 DBKOIJ010000000
VERSION     DBKOIJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.209"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(245..466)
                     /locus_tag="ACV5NJ_002657"
     CDS             complement(245..466)
                     /locus_tag="ACV5NJ_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486685.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACV5NJ_002658"
     CDS             complement(564..809)
                     /locus_tag="ACV5NJ_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486686.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACV5NJ_002659"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACV5NJ_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACV5NJ_002660"
     CDS             complement(2033..3034)
                     /locus_tag="ACV5NJ_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HHY6486687.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACV5NJ_002661"
     CDS             complement(3193..3459)
                     /locus_tag="ACV5NJ_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486688.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACV5NJ_002662"
     CDS             complement(3449..3805)
                     /locus_tag="ACV5NJ_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HHY6486689.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DBKOIJ010000205         3842 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000205 DBKOIJ010000000
VERSION     DBKOIJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.210"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(357..1424)
                     /locus_tag="ACV5NJ_002663"
     CDS             complement(357..1424)
                     /locus_tag="ACV5NJ_002663"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHY6486690.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1444..1923)
                     /gene="pssE"
                     /locus_tag="ACV5NJ_002664"
     CDS             complement(1444..1923)
                     /gene="pssE"
                     /locus_tag="ACV5NJ_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HHY6486691.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1940..2401)
                     /gene="pssD"
                     /locus_tag="ACV5NJ_002665"
     CDS             complement(1940..2401)
                     /gene="pssD"
                     /locus_tag="ACV5NJ_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HHY6486692.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2415..3665)
                     /locus_tag="ACV5NJ_002666"
     CDS             complement(2415..3665)
                     /locus_tag="ACV5NJ_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HHY6486693.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT         1191 a          804 c          502 g         1345 t
ORIGIN      
        1 gtattacaca atattgcaat tttatttctt aatatgatta tttcactcat tcattactgc
       61 cacatataca caacttataa ccaccccctg caaagtttag ggtaagttat cagataatat
      121 cttcctcttc atcatccaga atacaaaatc tatttattta tagaaatatc attaatgttt
      181 ttatataatg taccgtgctt gtactcttgc tctattacaa gcactaaaac actactattc
      241 atttgaattc ttacacggat aaaaatttac atcgtctatt tcaatatttt ttctctctat
      301 tttattcact taataagttc attacataat cgaaacattt tttattttcc ttgtcatcac
      361 tccttgctcc ctttttgata cagatttaaa attctatcaa agaaatttcc atcgttaaat
      421 ttcgttattg ccaaatcctt cgaacaacgt gaaaatttta cgtatttatc tttatcattg
      481 atttttagta ttgcagatgt caacgctgat acgtcaccag gttgaaacaa atatccattt
      541 tctccatcac gtactgctga agaaatatct cctacatctg tagcaattac aggcattcca
      601 taagaaatcg cctctagaat agatataggc aatccctcgt tataggacgg taaaatcatc
      661 acatttgatc ttctatagca ctcctccttt ttttctccca caacccaacc tgtaaattca
      721 aaatacgatt gcaaattata ttcttctatc aattctttga gcgatatttc ttcatcacct
      781 gctccagcaa tctcaaaaat aaactttttt tctggatatt gttctgcaat gtttttaact
      841 gcttctacca aatcgtgtac accttttcgc tttatcaaaa ctcccataaa cagaaatcgg
      901 cacatttttg aattccactt tatggtatat tctggaacta caatagaatt ttctacaaca
      961 acagttttac aatttggctc aatttcttta attatcttct tccatttttc tccaagtaca
     1021 atcgtggaat cagactctct taacagcaag cgaatctgat tttgtttttt tacagaaaca
     1081 ctatcatacc attttttaaa ttccgatcca tgaagatgga tgattacagg aatatttaat
     1141 aatctaaacc acttattcag cacatatttc ctactaaaac tccctctgta cgacatgtgc
     1201 atatgaataa tattaggttt actcaataaa gagataccta taattttagc aaatgcatac
     1261 ataaaatata aagatttaaa tattgcattt ccacccttat aactcggtat aaaagtcatc
     1321 tcgatattct ttttttccca attaaaattg agcatttgtg taattatact tgtaattcca
     1381 ccttttaccg agctgtcatt accaatcata agtactcttt gcataacgct ttactcctta
     1441 tatttatatt ttttctatta atacctctat cgaattaata atcgtttccg tgttagatac
     1501 atacttgtta aacatccgaa ctcttgtttc tttaacggca tcttctaaat tttctaattc
     1561 ataacaagga tagataatat tcatctcatc aaattgtcta attaactgca gttggtgatc
     1621 atcgacatgc tcaccatact tagccaatcg aggaacagca atgacccttt tacctttttt
     1681 tacggcccca attatagcac ctgtacctcc atgagtaatc accatttctg ctttttgcat
     1741 agtttcagta aactcatccc tatttagaaa tgctttatag gaataattta gtggtttata
     1801 atcactataa ccactctgtg caaatgtact ttcgattatt actccttttt caattaaatc
     1861 atcaatagct tttaacaatc tatcaaattg aaatttttgt gaacctaatg ttataaatat
     1921 catttttttc tcctattatt taatatatcc cacccaaata aatagcatta ggataaaatt
     1981 gtagcatagt ttcccactga acataaaatt gatctgcaaa atgatacatc aattttcctg
     2041 tctgagtagg gctggttact tttgcaaacg attcaatata aatcagtttt tttcccatca
     2101 tttttgttat taagcagata gggatcatag ctaaaacacc tgtacaaatc acgagatccg
     2161 gtttttcctt tatatatata ataattgatt tgaataaatt aataagtata gtacacaaaa
     2221 aaaacttttc ttttctattt acttgtctca tgtagtacgt tttttcgcct tttgactcag
     2281 ctgaatattc tgtctgttct gttataacaa aactctcata cttctccatc agtggtttta
     2341 acatcattaa ctgttcataa tgccccccag aggaagccgc aaaacatatc tttttgttca
     2401 tattaacctc tctatcagtc acgctgaaaa atatctctag tgtaaacttt ttctttaata
     2461 tcatccaagc aggtatcata tcgattggca ataatagaat cacacatcgc cttaaactgc
     2521 tctaaatcgt tgataacctt acttccgaaa aaagtctctc catctttcag tgcaggctca
     2581 taaatcacca cagtcgcacc tttagctttt acgcgcttca taactccctg gattgaactc
     2641 tgacgaaaat tatcactgtt gctcttcatg gtcagccggt atataccgat taaaactgga
     2701 ttttccctgt tgacatcata ctgattatat tcagcgtagt tgtaatagcc cgccatctga
     2761 agaacacgat ctgcgatgaa atccttacgg gtacgattag attcaacgat agcctcaatc
     2821 aaatttccag gaacatctgc atagtttgcc aacaactgct tggtatcttt tggcaagcaa
     2881 taacctccat atccaaagga tggattgttg tagtgagttc caatacgagg atcgagacaa
     2941 acaccattga ttatctgctg agtattcagc cccttcattt cagcataagt gtctaattca
     3001 ttgaaatagc tgactcgcag tgctaaatag gtatttgcaa acagctttac tgcttcggct
     3061 tcagtgaatc ccataaaaag agtatccacg ttctccttaa ttgcactttt ctgcagaagt
     3121 gttgcaaaaa catgagcagc atttaacaag cgctcatcag tcatatcagt accaacaata
     3181 atacgagatg gatacagatt atcatataaa gccttgctct cgcgcagaaa ctctggacta
     3241 aaaataatgt tcttactccc cgttgtctca cggactgatt cagtgtagcc aacaggaatt
     3301 gtagacttga tgaccatgat ggtatcagga ttcacgtcca tcacaacctc aataactgac
     3361 tcaacagcag aagtatcgaa atgctgagta cgactgtcat agttagtcgg tgtagcaata
     3421 acaacgaaat ctgcggattt ataagcgctc cttgcatcca aagtagcagt taagtcaagt
     3481 tctttttctg taagatactt ttcaatatat tcatcctgaa ttggagactt ccgtttatta
     3541 atcagttcaa tcttttcagg gataatgtct accgcggtta cctggtgatg ctgcgccaat
     3601 agtgtggcga tactcaaacc aacataaccg gtgccggcta ctgcgatgtt gtactttttc
     3661 ttcacaatat ctcctctttc ctctcttaag tatggagaat gttactccta cggctattca
     3721 tcatgtcaaa ataacacagc ttagaatgtt gataaaataa ttttccagaa tgtgataaaa
     3781 tctctttcat ttataaacca cacatctaat tttatctatt ctccacaaga catgtagagt
     3841 at
//
LOCUS       DBKOIJ010000206         3820 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000206 DBKOIJ010000000
VERSION     DBKOIJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.211"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(381..1028)
                     /locus_tag="ACV5NJ_002667"
     CDS             complement(381..1028)
                     /locus_tag="ACV5NJ_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HHY6486694.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1158..2096)
                     /locus_tag="ACV5NJ_002668"
     CDS             complement(1158..2096)
                     /locus_tag="ACV5NJ_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HHY6486695.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2587..3804
                     /locus_tag="ACV5NJ_002669"
                     /pseudo
     CDS             2587..3804
                     /locus_tag="ACV5NJ_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         1360 a          624 c          562 g         1274 t
ORIGIN      
        1 tttgacaggg ggctttttga tgtaaaaatt tctatcgaaa tttaaaaatt cgcttcactc
       61 atgttataaa gacttaaaat aaaataactc tttaaaatct tttgctagtt gttcttcaat
      121 attttttatt cggtgcatct tccaagtaaa gtataacaca ctagacttat ttactacgtt
      181 tcataagtca ttaatgcgtg tgctctgcga ggctagtttt tgtgcaagca caaaaaatgg
      241 actgaataaa tcagtccata agttcaaaac caaattcaaa atcaaaacca caagcaacca
      301 aaaaatgtgg ttgttatacg ttcataaatt ttatgatcac ttacgtgtat aaaattaaat
      361 tcactttcaa aatctaaaaa ctaaatccaa tcatctaccc tatgaattat atcttgaaat
      421 tcattcataa atagtgaagc atggtaacca tcacatacag aatgatgaag ttgcagagca
      481 actggtatat aaattttatt attctcacta taaaatttac ctatcgtaat aataggcaat
      541 aaaaagctgc tattgttacc aatatttaaa ttaaatgaac taaaatcaat ccaaggaatc
      601 attgaaatcg gtatggtgtt ttcaggtatc ggttttttag gaaacatttc ttctttatct
      661 ttatattcaa gcaagtcatt tttataatta ttataaaaag aaatgaagtt tttatcagat
      721 tcagtccaaa tgttagtaaa tttttcagtt tgcttattaa aaactgtata caaaggattt
      781 aacttatccc aataacctaa tttattctca ctattaattc ctgttctaaa cactttattt
      841 ttatttacaa cttccataat tgcataaatt aaagagggat aaatttcata tcctttcttt
      901 tttatcatat ctttaaacaa agtaatatca atttctttag taatgctata agtagtttgc
      961 tgattaaaat agtgttcaaa atattctttt ctatcccaat tttctaattc aataatatta
     1021 aaagtcatat ataacttcct cctaaatttt aaatttttat atttaggagg aataatcctc
     1081 tgattttttc atacgttatg tcacctcgta aatattaatt atactgaatt agcaattttt
     1141 atcaaataaa acttatttta cttccaaaac ctaaattcac gttgccaaaa atcaatctgc
     1201 ttttgcaatt gtttttcgtt cgcttttaaa gtcgatttca ttaattccgt taaatcaatt
     1261 ggagatattt ctctaatcaa ttttttaaat ttagtcttag tattcttact tagctttccc
     1321 cacatacttt cttcatgcaa caaagtataa accatagctt gctcattaat tttttctaaa
     1381 gtagcccacg caggtttcaa gatgtgtaaa tcattaaaac aatcattcca gtaatcaacc
     1441 atatctcttt ttaattcaac ttctacacgc cataaatgtt cagacacaac ttcaacatct
     1501 gcgttatctt tacgttcttg ttttttatta taaattctaa taaatctatc actatcacgg
     1561 acaccaaaat attttgtttc tggcttgcca ttacgaccat aaaaaacagt tttcttaact
     1621 gctttatcag tcattgcata gtaatcgctc aaatcatctt caaaatcaaa agctaagtct
     1681 aatcttgtaa aaccgtcatc ttccatgtag tcgataatat tttgttttaa ccaaatcatt
     1741 tcttcatgtg tgagtttatt gggattaaat tcaacacgca tattacgtct atcccaagta
     1801 tctgctttta ctttgtcata ttcgatataa actttttctt gaagtgcctt agctttaaac
     1861 tttgtttgaa gtatatccca aagtcgtatt tgtggctcta cactcataaa gtcagatagc
     1921 tttttagcat tagttttgtt caaatttcca acgattgtca tggcatcaaa acttaatgcg
     1981 ggttgagatt ttcccaaagt ttgaccactt aaccggctat tacttaaccg gctattagag
     2041 acggccggtt tttttagtga gatttctgca gctaattttt catcaatttt cttcattttt
     2101 tatcgctcct aaaataattt ttggaacgaa aaaaggacga gtggaatcgc aaaataaatt
     2161 tcgcctgtat ccattcgtcc attttgtttt gtattcaaaa tcaaaaattg atatactaat
     2221 catcgaatga ataacaagtg tggtttgtta ttccgggcga gaacgttggt agcgttgctc
     2281 gtcttttttt atgtcttttt ttcgttaaaa ctattttaaa actaaatata tcttaaagca
     2341 aataaattca aggggctaaa agcgcccctt aaaaacccct tgcctctgaa aagttttgtg
     2401 tttcctttcc gtcggtttca aaagctaatt tactcaaaaa aatagatcaa gggtaaatgc
     2461 actcactgcg ttcgcccttg agctatttta tttcgcaaat tttaacgcat ttgccgacga
     2521 aaagataaaa cacataaaaa tatacgcaga tatatttaaa acgtcttaga acgcaaaatt
     2581 ttacgtttaa aagctatgca aggactgtat gaagcaaaat tattgagtta tccaagaaca
     2641 gatacaccat ttattacaga gaacgaattt gcttatttaa aagcgaattt tggcaaatat
     2701 agcggttttt taggacttga tcttgaaatg gttcaaacag agcctagaaa gcgttatgtg
     2761 gacggtagta aggtacagga acaccacgcc attatcccaa caaaacaagt acctaccgaa
     2821 tctgcattag cgaaaatgga cgatttacaa cgaaaaattt atgctttagt cgttaaaacg
     2881 accgttgcca tgtttttacc tgattacttg tatgaagaaa ccaagataca aacgaaagta
     2941 gctgatttgc tgtttcaatc gatcggaaag acaccaaagc aagaagggtg gaaaattctt
     3001 ttcaaacaac aaaccaaaga agaaaaagag gacgttcaaa cgttaccact cgttatcatt
     3061 ggcgaacgtg ccgaagttgg tgttaagagt gttgaaaaag aaacgcaacc gccaaaagct
     3121 tttacagagg gtacattatt aactgctatg aaaacggcga ataaaacggt tgatgatgaa
     3181 gaagcaatca agattttaca agaagttgag gggattggaa cagaagcgac aagagcaagt
     3241 attattgaag cgttaaaaca aaaagaatat atccaagtga ttaagaataa gcttgttgta
     3301 actgaaaaag gaaaattatt gtgccaggca gttgaaagtc agcacctttt aacgagtgct
     3361 gaaatgacgg ctaaatggga aacgtattta aaaaaaatcg gtaaaagaga aggcaatcaa
     3421 gagaacttta ttacgaatat caaaaaattc attgttcatt tactggaagc tgtacctaac
     3481 gatatagaaa aactaaattt ttctgattac caggaacaga aagaaaaaga agcagaaaaa
     3541 agtattgtag gaaaatgtcc taagtgtggc aacaatattg tattaaaaaa atcgttttat
     3601 ggttgttcaa attatcctga atgtaagttt actttagctg aacattttag aaagaaaaaa
     3661 ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag aaaccctggt aaaaggaatc
     3721 aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt
     3781 gattttatct ctttttcaaa ataaacataa aagcccttta
//
LOCUS       DBKOIJ010000207         3692 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000207 DBKOIJ010000000
VERSION     DBKOIJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.212"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            52..1245
                     /locus_tag="ACV5NJ_002670"
     CDS             52..1245
                     /locus_tag="ACV5NJ_002670"
                     /EC_number="7.6.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary amine ABC transporter ATP-binding
                     protein"
                     /protein_id="HHY6486696.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            1238..2965
                     /locus_tag="ACV5NJ_002671"
     CDS             1238..2965
                     /locus_tag="ACV5NJ_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HHY6486697.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            3359..3556
                     /locus_tag="ACV5NJ_002672"
     CDS             3359..3556
                     /locus_tag="ACV5NJ_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HHY6486698.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            3558..>3692
                     /locus_tag="ACV5NJ_002673"
     CDS             3558..>3692
                     /locus_tag="ACV5NJ_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HHY6486699.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     L"
BASE COUNT         1270 a          596 c          810 g         1016 t
ORIGIN      
        1 gtcacttttt tcagaaagaa aaatgaattt acataaaaaa ggagaatttg tttggtaaaa
       61 gtagaagtaa aacatttaac aaagatattt ggtaaaaaga cacaagccgc tttagatatg
      121 atgaatgatc accagccgaa aacggagatt ttaaagaaaa ccggtgcaac ggttggtgtg
      181 tatgacgtga attttgacgt aaaagaagga gaaatcttcg tgatcatggg gctttccggg
      241 agtgggaaat ccacattgat ccgtctattg aatcgtctga ttgagccaac ttcgggcagt
      301 atctatattg atggagaaga tgtatcgaag ttaagcaaag aagaattgcg agaagtccgt
      361 cgtcataaaa tcaatatggt tttccaaaac ttcggtctgt ttccacatcg aacgatcttg
      421 gaaaatacag aatacgggtt agaagtccgt ggtgtaccta aagaagaacg acaagaaaaa
      481 gcagaaaaag cattagaaaa ttctagtttg ctttcattta aagaccaata tcctagtcag
      541 ttatctggtg ggatgcaaca acgggttggc ctagccagag ctttagcaaa tgatcctgag
      601 atcttgctga tggacgaagc gttctcggct cttgatccgc tgatccggcg agaaatgcaa
      661 gacgaattac tggacctgca agccaatgtt caaaaaacga ttattttcat tacccatgat
      721 ttgaatgaag ctttacggat tggggacaga atcgccttga tgaaagacgg cgaaatcatg
      781 cagatcggaa caggtgaaga aatattgacc aatccagcaa atgattatgt acgtgaattt
      841 gtggaagaag tcgatcgttc caaagtgctt acggctcaaa acatcatggt acctgcattg
      901 acgacgaata tcgaatctga tggaccaaac gttgcattga caaggatgcg taatgaagaa
      961 gtcagcatgc tgatggctgt tgacagaaag agacacctga aagggatcat cactgcagat
     1021 caagcgttag aagctaggaa gcaaaaacgt ccattgatcg acttcctaga tgaaaatgtg
     1081 acggtgattg ggaaagacat ggtggtaagt gatattttca atattattta tgactcaccg
     1141 actccacttg cagttgtaga ggatggaaaa ttaaaaggtg tcgtcatccg aggtagtgtg
     1201 attgaagcat tagcagaaac aagcgaggtg agtgaacatg aataatctat tgaactatca
     1261 attaccagtg gcaaactggg tagaaaatat cacagaatgg tttacgacaa ctttttctgg
     1321 cctattttca tttttacaaa cgattggaca ggcagtgatg tcggggatta caaatttact
     1381 gttagtgatt ccagctcctt tgtttatctt gttattgacg gtagcagctt tctttatttc
     1441 taagaaacgt ccaggactta cgttgttcac attaatcggt ctatggttta tttataacca
     1501 aggtctatgg aatgatttga tgaatactgt cacacttgtt cttttatcta gtgtgatctc
     1561 gatcatcatt ggggtaccat tagggatctt gatggcaaaa agcagtaaag cccaaagtat
     1621 tatcaaaccg atccttgact ttatgcagac aatgccagga ttcgtttatt tgattccagc
     1681 agttgcattt ttcggtatcg ggatggttcc aggggtattt gcctcagtca tctttgctct
     1741 gccaccaacc gttcgtttta cgaatttagg gattcgacaa gttcctaaag aattagtaga
     1801 agcttctgat tcattcggga gcacaagtcg tcaaaaacta ttcaaactag aattgccttt
     1861 agctaaaagt acgatcatgg cagggatcaa tcaaacaaca atgctgtcac tttcaatggt
     1921 cgttattgcc tctatgatcg gtgcacccgg cttaggacgc ggcgtattat cagcattgca
     1981 aagagcacaa gtcgggaatg gatttgtcaa cggggttgca ttagttatct tagcaatcat
     2041 cattgaccgt tttacacagc atttgaacca gccaaacaat aaaaaagctg caggagcaac
     2101 aacacagaaa agtaaaaaac gtcaaggact gatcattggt gctgtagtag tagtgatctt
     2161 aggtgcgatt ggtataggga gtttttcatc tgccaaagaa acaaaacgta tcaacttatc
     2221 ttatgttgaa tgggatacag aagttgcttc aacaaatgta gtcggtgaag tattgaaaca
     2281 aatgggctat gatgtaacga tgacgccatt agataattca atcatgtgga aatctgtttc
     2341 aaatggcgag tcagatgcca tggtttcagc ttggctacct aaaactcatg gttcacaata
     2401 tgcacaatat aaagatcaag tcgaagacct aggtgccaat ttgacaggag caaaagttgg
     2461 tttagcagtg ccagcctaca tggatgtaaa ttcgatcgat gagttaaccg atcaggcagg
     2521 taagaaaatc ataggaatcg agccaggagc tggtgtagtt acggctgctg aaaacacgat
     2581 ccaaaaatat gacaacttga aagactggaa agtagagaca tcttcttcag gagcaatgac
     2641 cgttgcacta ggtcaagcga tcaaaaaaca cgaaccaatt gtagttacag gatggacacc
     2701 tcattggatg tttgccaaat atgatctgaa atacttagaa gatcctgaaa acggaatggg
     2761 aagcgaagag cagatccata cgatggttcg aaaaggattg aaagaagacc agccagaagc
     2821 ttacaaagta ttagacaatt tccattggtc agaaaaagat atggaaaaag ttatgttgga
     2881 aatcaacaat ggaaaagatc cacagcaagc agcaaaagac tggatcaaag agaaccaaga
     2941 attagtcgaa agctggaaaa aataaaaaga cagacacgaa ataaatggag cttccaaaat
     3001 tggatcaaat atccaaattt ggaagctcca tgttcgtctc tatctttttt gtctataaaa
     3061 aaagagaatg cgtagctcat cttcgaaacg tccaaagacg ccgacaaact attatgaatt
     3121 tatgggtgcg agtcaaagag atcgatcgta ggtatgaaag actaatgtga ttaaagttta
     3181 ttatgtacga agtgcattct caaaaaatat taaagaatat acatagttta tatacttaaa
     3241 agtgattaaa ttcttattca ggaattattt tgctacgttt caagaaggaa ttgttttttg
     3301 aaatttaatg aatgttagaa taatagtcta aaaacattaa tttaggggtg attagattat
     3361 gaaacattta aaaattttgt ctattaaaga gacacaactt atctatgggg gtaccactca
     3421 tagtggaaaa tattatggaa atggagtgta ttgcactaaa aataaatgta cggtcgattg
     3481 ggccaaggca actacttgta ttgcaggaat gtctataggt ggttttttag gtggagcaat
     3541 tccagggaag tgctaaaatg aaaaaaaatg ctaagcaaat tgttcatgaa ttatataatg
     3601 atatatctat aagtaaagat cctaaatatt ctgatattct tgaggtttta caaaaggtat
     3661 atttaaaatt agaaaaacaa aaatatgaat ta
//
LOCUS       DBKOIJ010000208         3664 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000208 DBKOIJ010000000
VERSION     DBKOIJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.213"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..277
                     /locus_tag="ACV5NJ_002674"
     CDS             <1..277
                     /locus_tag="ACV5NJ_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HHY6486700.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ACV5NJ_002675"
     CDS             420..641
                     /locus_tag="ACV5NJ_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486701.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ACV5NJ_002676"
     CDS             657..1499
                     /locus_tag="ACV5NJ_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486702.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ACV5NJ_002677"
     CDS             1562..3364
                     /locus_tag="ACV5NJ_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HHY6486703.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="ACV5NJ_002678"
     CDS             complement(3423..>3664)
                     /locus_tag="ACV5NJ_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HHY6486704.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DBKOIJ010000209         3661 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000209 DBKOIJ010000000
VERSION     DBKOIJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.214"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            39..791
                     /locus_tag="ACV5NJ_002679"
     CDS             39..791
                     /locus_tag="ACV5NJ_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HHY6486705.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            788..1702
                     /gene="pfkB"
                     /locus_tag="ACV5NJ_002680"
     CDS             788..1702
                     /gene="pfkB"
                     /locus_tag="ACV5NJ_002680"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HHY6486706.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1720..3651
                     /locus_tag="ACV5NJ_002681"
     CDS             1720..3651
                     /locus_tag="ACV5NJ_002681"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HHY6486707.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1180 a          659 c          836 g          986 t
ORIGIN      
        1 aaaaatatgc tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgcagac
       61 aaaaaatcct agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc
      121 ttttgaatgc ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt
      181 ttctagaaag agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata
      241 tgagtgaaaa atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg
      301 aaaccattaa tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga
      361 ttccttttct gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa
      421 atttagttga tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta
      481 atgctgtttt aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat
      541 ttatgggaat gaacggtgcc catcttgaac aaggattcac gactccggat ccagaagagg
      601 cagctgttaa acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata
      661 ccaaattcaa ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa
      721 ccgagcgttg tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg
      781 caatacaatg atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga
      841 tgggttaaaa ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa
      901 agggatcaat gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt
      961 tttaggaggg ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc
     1021 tttgttcaca accgtaaaag aagatacaag aatcaatatc aaactaaaat cagacactga
     1081 aactgagatc aatggcttag gcccatctat ttcagatgaa gaaatcgatg agttgaagca
     1141 agcattagga acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccagctag
     1201 cctgcgaaaa ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt
     1261 cgtgattgat acaacaggtg atgacctaaa aaatgcactt gttaagaaac cgttgttgat
     1321 caaaccaaac aatcacgaat tagctgagct ttacaacaca accttccaat ctgtagaaga
     1381 aatcattccg tatgggaaaa aattactagc tgaaggggca cagaatgtgc tgatctcaat
     1441 ggctggagac ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt
     1501 tcgcccattg aaaaattcag taggtgcagg ggattcaatg attgctggct ttattggggc
     1561 ttacagccgt acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc
     1621 aacagctttt tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt
     1681 tgaaatcaca aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg
     1741 gtcaaagaag cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa
     1801 atggtccaaa aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga
     1861 attctagccc gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac
     1921 gcgaaaaaca gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta
     1981 gattatgaag ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa
     2041 ggcgcaaacg acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca
     2101 gatttcgtag gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa
     2161 acagctgaag agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa
     2221 gcttctgctc atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc
     2281 gctcacactt acatggcaga agatgctttg aagaaaaaag cgaaagaaat gggtgtggag
     2341 atcaaagtcg aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc
     2401 gcccgtgcag aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac
     2461 ggaaaagaac tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagttgatc
     2521 aatctagccc ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca
     2581 gaagaaggaa atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc
     2641 gtatcacaca tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg
     2701 atcgaccaat ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa
     2761 gctgctagct ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta
     2821 gcaggattta ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct
     2881 ggtgcattag ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta
     2941 gctggttatg tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat
     3001 ggaattaaaa cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg
     3061 ttgctggtca atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt
     3121 atgagtggtt cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat
     3181 ttaggcggtc caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact
     3241 gttgcaactg gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca
     3301 ttggcaattt tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat
     3361 gctggtttga cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt
     3421 gcagcagctg acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc
     3481 ggattagtcg gagctttcgg aatcaaatta ctggctcctc acggcgggat tttcgttgta
     3541 ttcttactga gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct
     3601 ggtgtgattt atggtttatt gagaaaagct cctgcagtag cagaagcata gaaataaaaa
     3661 a
//
LOCUS       DBKOIJ010000210         3603 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000210 DBKOIJ010000000
VERSION     DBKOIJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.215"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..186
                     /locus_tag="ACV5NJ_002682"
     CDS             <1..186
                     /locus_tag="ACV5NJ_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6486708.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACV5NJ_002683"
     CDS             329..553
                     /locus_tag="ACV5NJ_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486709.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACV5NJ_002684"
     CDS             568..1437
                     /locus_tag="ACV5NJ_002684"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HHY6486710.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACV5NJ_002685"
     CDS             1418..2152
                     /locus_tag="ACV5NJ_002685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHY6486711.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACV5NJ_002686"
     CDS             2185..3093
                     /locus_tag="ACV5NJ_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HHY6486712.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACV5NJ_002687"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACV5NJ_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          790 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 ttg
//
LOCUS       DBKOIJ010000211         3540 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000211 DBKOIJ010000000
VERSION     DBKOIJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.216"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..470)
                     /locus_tag="ACV5NJ_002688"
     CDS             complement(<1..470)
                     /locus_tag="ACV5NJ_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HHY6486713.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQ"
     gene            complement(485..2206)
                     /locus_tag="ACV5NJ_002689"
     CDS             complement(485..2206)
                     /locus_tag="ACV5NJ_002689"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486714.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            2306..3067
                     /locus_tag="ACV5NJ_002690"
     CDS             2306..3067
                     /locus_tag="ACV5NJ_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HHY6486715.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         1158 a          609 c          683 g         1090 t
ORIGIN      
        1 aattgattga tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga
       61 ctaatgatgt cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt
      121 tttgtgattt cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt
      181 tcctgaataa atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg
      241 aacaacaatg cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt
      301 tctgataaga gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg
      361 ttcccgaata aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt
      421 ttccaaaaac gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt
      481 ctttctatat tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt
      541 cttcatgagt accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt
      601 gttggattgt actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat
      661 gaagggctgc tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga
      721 atacatagat ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc
      781 cccctgaata attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt
      841 ccactagttc ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat
      901 attcatttcc catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag
      961 ggacataact gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac
     1021 caagggttac ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac
     1081 tttttccaga tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat
     1141 caatatcttc tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag
     1201 cagctgcatt tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa
     1261 tttcgatctc tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga
     1321 taatgaccaa taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga
     1381 ctacagcagt cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc
     1441 acaaaataga agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa
     1501 atattcggtt cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct
     1561 tttcttcata tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc
     1621 tcaatgtgat ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc
     1681 gtttcatcag tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg
     1741 ttagtaatag agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg
     1801 gtaagatcat ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca
     1861 ttcttgtcaa aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga
     1921 atttacggaa atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa
     1981 agtaactaga ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc
     2041 caattttttt tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta
     2101 ctaattttgg tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga
     2161 gagcgagtcc tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt
     2221 tttcctgata cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa
     2281 taaactgaaa aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga
     2341 actatttgat ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc
     2401 aagtagacaa gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta
     2461 tgatatcgtt ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg
     2521 aaaaacattg acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga
     2581 actggtggtg atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt
     2641 gatgatcgat acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat
     2701 ctcaacggag tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt
     2761 tgggatgatg agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc
     2821 tagtgaagta catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta
     2881 tcgtcgattc ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat
     2941 cagagaaaga atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt
     3001 gacgaatacg gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa
     3061 aaaataggaa atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa
     3121 aaattttctg gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa
     3181 ttttagtttt ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata
     3241 tatacgatgt aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt
     3301 ataattcatg gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt
     3361 gtatcagatg agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt
     3421 gttgcttaca ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac
     3481 atatttttta atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga
//
LOCUS       DBKOIJ010000212         3501 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000212 DBKOIJ010000000
VERSION     DBKOIJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.218"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            137..748
                     /locus_tag="ACV5NJ_002691"
     CDS             137..748
                     /locus_tag="ACV5NJ_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HHY6486716.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACV5NJ_002692"
     CDS             821..1750
                     /locus_tag="ACV5NJ_002692"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HHY6486717.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACV5NJ_002693"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACV5NJ_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740602.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..>3501)
                     /locus_tag="ACV5NJ_002694"
     CDS             complement(2693..>3501)
                     /locus_tag="ACV5NJ_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HHY6486718.1"
                     /translation="YGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIP
                     LKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFI
                     TDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKA
                     ALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKK
                     YSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         1157 a          544 c          673 g         1127 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac a
//
LOCUS       DBKOIJ010000213         3491 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000213 DBKOIJ010000000
VERSION     DBKOIJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.219"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(114..791)
                     /gene="nth"
                     /locus_tag="ACV5NJ_002695"
     CDS             complement(114..791)
                     /gene="nth"
                     /locus_tag="ACV5NJ_002695"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HHY6486719.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(798..1496)
                     /locus_tag="ACV5NJ_002696"
     CDS             complement(798..1496)
                     /locus_tag="ACV5NJ_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HHY6486720.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1764..2948
                     /locus_tag="ACV5NJ_002697"
     CDS             1764..2948
                     /locus_tag="ACV5NJ_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HHY6486721.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2954..3397)
                     /locus_tag="ACV5NJ_002698"
     CDS             complement(2954..3397)
                     /locus_tag="ACV5NJ_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHY6486722.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          890 a          706 c          591 g         1304 t
ORIGIN      
        1 ctagctactg agtatgtaca tttacttttt catgcaaaaa agataaacga aacagctttt
       61 ctccataata acaagataat tcatacattt atttgcaatt aaagaaacca cgtctacagg
      121 cgtggtttct tttttagcgc cttttctttt agacgcatcc gatttttccc atcttgacat
      181 attgatagca aaggacatac ctcgcacttg ggatttcttg ccgtgcaatg atatctacca
      241 aaaaaaatca gtgtatgatg tgtctttacc cataattctt gtggtacttt ccgcatcagt
      301 gtttcttcta cttccatgac cgtagcatct agtttgcaga tcctcaggcg ttttgagaca
      361 cgttccacgt gggtatctac tgcgattgct ggtatcccga atgcatcccc caatacgaca
      421 tttgctgtct ttctgcctac accaggaaga gacatcaatt cttctcttga cgttggcact
      481 tgcccatcga aacgttcaat cagctgctgt gcacacgctt tgatgttttt tgctttattt
      541 cgatacaaac caatcgtttt tattttaaga atgatttcat cgattgaagc ttctgctaac
      601 gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg ctttattcac tgatacatct
      661 gtcgcctggg cacttaagat cactgcaatc aataactcaa atggattatt gtgcttgagt
      721 tctccatgtg cttcgggaaa cattccatac atcgtttcta gagcttccat tgttttttgt
      781 ttgcttaaca tattccgcta ctcctccaac caatttttca gtgagacttt cggcaattct
      841 tcttgttgag ctgactgttc tacttctctt tgaagaagct gctgttttct tcgtttttgg
      901 tcttcttcca cttgttgttt tgtacgtaga ttttttcttt cccacgatag caaaatgcgg
      961 tcaacgtaat taaaactata agcctgattt aatacggctt cccgcagtgc tagcttcaaa
     1021 atttccggct gataatggtc ttcttctagc cattgtccga tccgctggaa ctcgatcgaa
     1081 gaaagcgggc gtccaaattc ttgttcaaat aattgataca ctgactggat ttctttttct
     1141 tgtgtttttt cttcgtattt tttttcttgt gtttttaaat actcacctaa tagatcatag
     1201 atggggtaaa gattatatcg atctgctttt ttaccgttga caatcgtcgt ttctatcttg
     1261 ataaatccat ttgttacgag acgatccagt atttgaaaaa tctgatcttg tttgattccc
     1321 atatccatag caatcatctg caaatcaggg aaagtatctc cttcaagctg tgccatatgc
     1381 aactgtagaa caaataaaaa ttcttcagat tgtagaccga tcctatgata atttttaagt
     1441 aaaagattag atatgactgt ttgtccagcg cttaagtaat tttctaaatt caacatgatt
     1501 tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg gaagcaagaa caggtttctt
     1561 ttctatttac ttcactgatc ggtcagattt acaatgtcgt ctacttgatt tttgtttgtt
     1621 catgtttttg atgcgtatct tcacttttcg gaataaataa attaagtaga tttcgctgtt
     1681 ccttagaatg ttgacaagga aacaaaatta ctatacacta tttttgtttc caagttaaca
     1741 ttattgcata aaggagtttt tacatgtaca gtttcacaaa taaacattct aaagtttttg
     1801 gatacgtctc tgtcttattc actggattag gatttactat cgtcagtcct gtacttccgt
     1861 ttttaacgct gccttattct cactccatac atcagcaagc tttctatatc actttgctca
     1921 tgtctgttta tgctctagct gccttcttat ctgcccccat tctcggtagc ttaagtgatc
     1981 attttgggcg tcatccgatc cttattatca gtttattagg atcaagtatc ggatatttgc
     2041 tttttggttt aggaaattcg atatggatgt tgttccttgg tcgtatcatt gaaggattga
     2101 cggcaggaga aatctccacc ttatatgctt actttgccga catcacggaa cctaatgaaa
     2161 gaaccaaagt ttttggatgg atgggtgctt tagtcggtat aggaacaacg ctgggtccta
     2221 ttataggggg tctattagct gagttgggga attctgttcc tatatttatt ggagcactct
     2281 ttactttctt aaatgccgtt tatggctaca catttatgct agaaagcctt ccaataaaaa
     2341 aacgttcagt agatttaagt ttctcccatg taagaccttt tcaccaatta caacaattat
     2401 tcaagatttc atccgttatt cctttattga cagcaggttt tgctgtttgg ttggcagctg
     2461 gctctcttca atctatcttt tctcaatttt cgattgacgc ctttcagtgg aaggctgggt
     2521 tggttggttt aagcttctct ttgattggaa tcttggatac tgtctcgcaa ttgttcatta
     2581 tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac aagaataggg atgttcagtg
     2641 aaatcctcgc ctatctattc atcacattat ctggaattct tctactgccg attttgtttt
     2701 tatttgggat tatctgttat ggtttcggtg attctatttt tactcctgta ttcaatggcc
     2761 aactatccaa ttctgtctca gataaccagc aaggtcttgt aatgggcggt acccaaagta
     2821 tacaatctct atcgcgcgtc atcggcccgc taatcgcagg acagctttat gctgcagcac
     2881 catttcttcc gacggtattc ggatttttgc ttatgatttt tgcctattat cactacaata
     2941 aaaaatagta gcttcatttg ctacttcgta taaattctct gtatcgaccg ataaaacgaa
     3001 atactgtgtc aaattcttct tttgacatat tttcaaatac agtagcatct cgctgtaccc
     3061 atttttgatg gagttgctcg tgtctattcc ttattttttg tccgattggc gtcaattgaa
     3121 aatagatttc ttttttattc tcttcgcttt gataagtgtt tatgatccct ctagctatca
     3181 gtttttttgt tagcttactg atggctcctc tagtcatgta actagcattc gctaattttg
     3241 tcacgttagg atcagggatt ttcccgatat gttcaatcac ttctagttca tttagagtaa
     3301 atcctgcaaa ttctttttcc atttctggtt tattacgatg aataatctca ttagctaaat
     3361 ccagatattc ttctattagc agttgtcttt tatccatttc tacaccttct ttttctttca
     3421 ttatattgtt tttttgtttc ctattcaaca aagataaaaa tattttgtag gaaaacccaa
     3481 tctgaaacca a
//
LOCUS       DBKOIJ010000214         3462 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000214 DBKOIJ010000000
VERSION     DBKOIJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.220"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(37..750)
                     /locus_tag="ACV5NJ_002699"
     CDS             complement(37..750)
                     /locus_tag="ACV5NJ_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486723.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(940..1530)
                     /locus_tag="ACV5NJ_002700"
     CDS             complement(940..1530)
                     /locus_tag="ACV5NJ_002700"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HHY6486724.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            2014..2241
                     /locus_tag="ACV5NJ_002701"
     CDS             2014..2241
                     /locus_tag="ACV5NJ_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HHY6486725.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2257..3422
                     /gene="hemW"
                     /locus_tag="ACV5NJ_002702"
                     /pseudo
     CDS             2257..3422
                     /gene="hemW"
                     /locus_tag="ACV5NJ_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
BASE COUNT         1141 a          577 c          665 g         1079 t
ORIGIN      
        1 gctgtcagct tctttttgta taacatatta gaaagtctaa ttatctataa ctgtcacatc
       61 gattaactgt ttcacgtcat tgggatttcc tgtaggaata gctactacag atgcattatc
      121 aatgatttct tgagttgttt ctctaatgaa cgctggataa gatgtttgat ctgcaggcat
      181 ttgccaatgt gaaggaactg gataactagt gattgtcccg tcatggttac ttgaatacac
      241 taaaactccc tggtaactgt atttcccagt gagcataacc acatctttcg gatagactgc
      301 actccctgca tcataagggg cgataggtgt tcctgcagga taatgaataa catgtaattc
      361 gaaatcaccc gatgtatctc taaaagtatt acctctaata gctagccaaa cacgtgcgta
      421 ctcaatttgt agatcagaat atcctgctaa cggatctatt gactgagaag actgtgagga
      481 ggttgtgtct attgttgaag aagaaatatt tgtctcaact gtactactag atgtagaaga
      541 agtattgggc tctgagactt tactgcttgt tgaagatgta ttcgtttcag tagaagctga
      601 tttctgagta cttgattttt cagctgaact ggtttgtctg attgaacttt tgtctgtggt
      661 cgcagatgga tcactatttt ctttactagg agacgtacat cctacaagga ataaagagac
      721 caacaataaa ataaaaccta actttttcat ataaaatacc tcattatttt tttaatacca
      781 gtatagcata atcctgaatt tttgaatccg taaaaccttg tctatgaata taaatagagt
      841 gtatcttctt tgataaatgt cagaaaaagt gtgacaaata atcatcagta aaattacttg
      901 tcacactatt gataaggagt atttaattga agagaatgat taggagaaag gatctccgta
      961 ctttttatag taatcataga atccaagttt gtatagacga atcgtttttt ctcttatttc
     1021 ttctgtttca aaatggactg cttgaagaat caagtctgtg aattctatag gagctgttcc
     1081 attcgcagta atcaaattac gatctttcac agcttgttgt tctataaaat cttctttatt
     1141 ctgataattt tccgattcta accaaggaaa ttgcgcattt ccggtgtgtt tgtaaccttg
     1201 caataatccg ctctgagcta aataatcaac tgccccacaa atagcagcta ctggtacgtc
     1261 tgtttttaac aatttatcga ttatttgata taaatgatga ttttttatgt cccatgaatt
     1321 gccaccgatc ataataaaaa ggtcaacttt ttcaggtatt tgttcgatta agtaatcgat
     1381 ttttgttttt aatcctccaa tggaagtgac ctctgattgg atagaagcag ttttgttttg
     1441 ccatttctca ttttggttta gtgtgctagt aatataagca ccttcccaat cggcatattc
     1501 ctctaataga aacgtaatcg cagttttcat tttcaagctc tcctttacta tctataacaa
     1561 ggacaacagt tttaacctac agacattcat taaaattggg tagcgacagc gaaaacaaca
     1621 attcttaagt tttagacaga gtatacagat agtcaactta attaaatacg aactaaataa
     1681 aaatgacagg taatcatttt tgtttaccag cattataaca ttttgcatac atggatgaca
     1741 aaagaaccaa ttcccacttg cttgagtaca atattaaaag aaactggaag gcaaaaagta
     1801 attatagaaa cgaaaaataa atcatatcgg gcactcagac atgaaaacaa ccctagagat
     1861 atataactag gtaaccagtt taatgaaaga aaaaatcatc caagaagtcg attcttggat
     1921 tttttagtgg atttgttcgc caaatacgtt tagtatgcgt atatgagagg ttgtaagcaa
     1981 agcgttgtgg tctgagcact taaggaggaa tctgtgaaaa tagcttttca tatttttgca
     2041 gattttgact taatgccgaa ggtcagcttt gcgattaccg tttatcgaga ggctgtaaaa
     2101 gcaccgtgta gctccgatga caataagcaa gcatttttag agctaggttt ccttgattct
     2161 aaatatgcgg cttatttcaa tgagttggtg cttgaacgcg attttaagaa ggtcgaaaac
     2221 aggcacgact tttcgacgta aggagaaaga atttcaatga tagaaacaat aaatcccgca
     2281 gaaaacaagg tttctgcgta cattcatatc ccattttgcg agcatatctg ctattactgt
     2341 gattttaata aagttttttt agaagggcag ccagtagatg agtatgtgga aatgctgctg
     2401 aatgaaatga aaataatgct agaaagtcat ccgacagcga atttagaaac actttacgta
     2461 gggggcggga caccaacttc tttatctgct aaacaacttg atcgactgtt atcaggtgct
     2521 agagagatcc tgccttttca aaatggtaat gagtttactg tagaagcgaa tccaggtgat
     2581 ttgacccgtg aaaaacttca agtaatgaaa aactatggcg tcaatcgttt atccatggga
     2641 gtgcagacat ttgataatcg cttattgaaa aaaattggac gtaaacacac cgcagaagat
     2701 gtgtatcaaa ctatgcaatt tttagaagca gaaaatttta ccaatgtaag catcgatttg
     2761 atttatgcat taccgggaca aacactagaa ggatatctgg atactttgga aaaagctttg
     2821 gctttggatc tgcctcacta ttctttgtac tcattgatcc tcgaaaacaa aacaatgttc
     2881 atgaactggg tacgacaagg aagactcgaa ctgcctgatc aggaaacgga aaccagaatg
     2941 tttgaagaga cgattgaagc aatggaaaag catggacgtc accaatatga aataagtaac
     3001 tttgggctct ccggacatga atctcaacat aacctcatgt actggaacaa tgaccactat
     3061 tttgggtttg gagctggtgc tagcggttat cttggagata ttcgttaccg caacaaagga
     3121 ccaatccaac actatttacg cccattacga gcaaatgaac gcccggtatt ggaagaagaa
     3181 aaattgacac gaaaaaatca aatagaagaa gaaatgttct taggactaag aaaaaaacag
     3241 ggattactaa aaaacatttc tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg
     3301 atgtgctaag ggaattagag caagaagggt tacttgttaa tgaagaagac cgtgtttatt
     3361 tgacaccaag agggaccttt ttggggaaca atgtgtttga gcgctttttg tttgaaaaat
     3421 agtttaaaga atatttgttt tttatctgaa tttaaataaa aa
//
LOCUS       DBKOIJ010000215         3435 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000215 DBKOIJ010000000
VERSION     DBKOIJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.221"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..2177)
                     /locus_tag="ACV5NJ_002703"
     CDS             complement(<1..2177)
                     /locus_tag="ACV5NJ_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HHY6486726.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     STNNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3142)
                     /locus_tag="ACV5NJ_002704"
                     /pseudo
     CDS             complement(2849..3142)
                     /locus_tag="ACV5NJ_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          972 a          756 c          539 g         1168 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgtactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataac
//
LOCUS       DBKOIJ010000216         3422 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000216 DBKOIJ010000000
VERSION     DBKOIJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.223"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            149..388
                     /locus_tag="ACV5NJ_002705"
     CDS             149..388
                     /locus_tag="ACV5NJ_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HHY6486727.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            452..505
                     /locus_tag="ACV5NJ_002706"
     CDS             452..505
                     /locus_tag="ACV5NJ_002706"
                     /inference="COORDINATES: protein motif:HMM:NF018061.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HHY6486728.1"
                     /translation="MRNVDKTSTVLKQKSSP"
     gene            complement(543..1715)
                     /locus_tag="ACV5NJ_002707"
     CDS             complement(543..1715)
                     /locus_tag="ACV5NJ_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HHY6486729.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            complement(2834..3175)
                     /locus_tag="ACV5NJ_002708"
     CDS             complement(2834..3175)
                     /locus_tag="ACV5NJ_002708"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HHY6486730.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3175..3405)
                     /gene="mazE"
                     /locus_tag="ACV5NJ_002709"
                     /gene_synonym="pemI"
     CDS             complement(3175..3405)
                     /gene="mazE"
                     /locus_tag="ACV5NJ_002709"
                     /gene_synonym="pemI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemI/MazE family
                     antitoxin"
                     /protein_id="HHY6486731.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT         1093 a          599 c          571 g         1159 t
ORIGIN      
        1 tctttctcaa aataaacata aaagcccttt aaagagggct tttatatatt aatcacaaat
       61 cacttatcac aaatcactta tcacaaatca caagtgattt gtgattgttg atgataaaat
      121 aagaataaga agaaatagaa agaagtgagt gattgtggga aatttaggcg cacaaaaaga
      181 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg
      241 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
      301 aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcatta
      361 tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa
      421 gaaggaggga ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt
      481 tttgaaacag aagtcaagtc cataatcctg tgtaaaatac tatacaatgt tttaccggtc
      541 tctcattgat caaacttaat atattttctt gtagaactga gccattcgtc atgaaggtcc
      601 ataaggacag ctccaattaa tcgattggca gacgttcggt tgggaaaaat ccgaataatc
      661 ttttctcttc tgcggacttc ctggttcagt cgttcaagaa ggttggtgct ttttagccga
      721 ttatgactat ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt
      781 tcgcaggctt ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct
      841 gttcgagcga gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt
      901 tttgaattct tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc
      961 tgccaacttg cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca
     1021 gaaatgatga gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac
     1081 catgtgtcat cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct
     1141 tctgttatcc cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact
     1201 tttatgtaga gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc
     1261 tgccattcgt tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat
     1321 acagattttc cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa
     1381 acatacatct caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac
     1441 accgtcggtg aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca
     1501 cgagtcgtaa agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt
     1561 tcatagtcat cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca
     1621 gtggttaaaa tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc
     1681 tcttcgttgt tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt
     1741 gtggttaaaa acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat
     1801 tatatggact ttatcaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta
     1861 atgtatcatt aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc
     1921 caaccaacat ataccctata aaagctaata agatggttag gaatccttta gaatttttac
     1981 tcattcatag acacttcctt aaataacttt tattatagaa ggtattgtaa ataataatat
     2041 tgacaaaaag tgactcaaaa taaaaaaaac cttacattat actggaatga actgtattga
     2101 tagtgtacct aaaagctcta ctgacataag atcgtgtaat cggtaaagta attttattaa
     2161 ttatacggga tgtaatcaat tagagaataa actaatcata aaaggtactt ttacattcat
     2221 aattgtcgct ttgagattct actttggttc actatctgac ccaattgcac attttcgatc
     2281 attacaagta ttggtgtcgt tagaacaact aaataaaaac tagcggtaga tttttcagaa
     2341 tctatgctaa tttctgtatg gatgttccag tgatggcaaa tttctctttt cattgttttt
     2401 cttgtcgcat gatatgcctt tttgtgttat tcagttaaac tatagaaatt ctttcctaga
     2461 cagatcttga tcccaaattt tacgtttttg agcatcgata tagtttaaaa tgacattact
     2521 catatctaaa gagtttagat tttttaggac taaaactgtc agttgttctt tttcttcttt
     2581 ctgttaaagt attgtcgcga cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt
     2641 ttgtgacaca aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt ttaaaatgct
     2701 atatattctt tagtttaaga tacggaaatt aatgccatcg accacactag tagaatgaac
     2761 gacatttact tggccttttt ttaatttcgt tatgtttagc atataaaaat attatagagt
     2821 ttacttacaa tgcttaaaac atatactgta taatctgatc aattttagcc atgtcttgca
     2881 atggtaagtt ttctattttt ttgagttttc gttccttaaa atcaagagat tttaattgtg
     2941 taataattac ttgtcctttt atatctagct cttcaggtaa tgagtaacgt gtcggtaaat
     3001 tctttatagt ggaagtaatt ggacatatga ccgaaaacat tgttttacgg ttaaattcat
     3061 aacgagagac gactagtcct ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa
     3121 aatcaatcca tacaatatca ccttttttag gaatataaat tctttcttct gacactataa
     3181 aatttctctc ccttcaggag ttaaatcttc ccattcgtct ttctcataat actcggcctc
     3241 ttccccacca ctaaatggat cttcaatttt tggaactaac gtaattgttc catcatcaga
     3301 atacacaacg atatattctt ggttttctga aggtttcttt ccgttatcag aaggtaaagt
     3361 taccactaca gaacttcctt gtaatctaga ttttgtaaca agcattatca tcactccctt
     3421 aa
//
LOCUS       DBKOIJ010000217         3388 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000217 DBKOIJ010000000
VERSION     DBKOIJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.224"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1319
                     /locus_tag="ACV5NJ_002710"
     CDS             <1..1319
                     /locus_tag="ACV5NJ_002710"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6486732.1"
                     /translation="FFTSVVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKN
                     KQISEQNQYELGVLNNRMEEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFM
                     NCAIYPAIRFINQLAFIISAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVI
                     NSIQSAMAAIDRIFEILDETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQV
                     DFSVKPKQTVAIVGPTGAGKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFG
                     MVLQNTWLFEGTVADNIAYGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENG
                     TLSQGQQQLLTIARVILANPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAH
                     RLSTIENADMILVMKNGDIVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(1700..3253)
                     /locus_tag="ACV5NJ_002711"
     CDS             complement(1700..3253)
                     /locus_tag="ACV5NJ_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HHY6486733.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
BASE COUNT         1061 a          661 c          619 g         1047 t
ORIGIN      
        1 aattttttac ttctgtcgta acaatagctg ctgctggtct gatgctgttt tatatcgaaa
       61 gcaagcttac tttgctcgtc ttattgctga tcgttggcag ttcaattgtt acaactaaga
      121 ttgcgaacaa aaacaaacag atttctgaac agaatcagta tgaactgggt gttttaaata
      181 atcgaatgga agaaatctta gctggtaatc tagtggtcaa aacattcaat cagcaagctc
      241 atgcagaagc tgctatacac gaagtaaata aaaagcaata caacgctttt aaacgtgctc
      301 aatttatgaa ctgtgctatc tatccggcca ttcgtttcat taatcaactc gccttcatca
      361 taagtgcggt attcggtgct atgcttgttc tatcaggtgg tgtgacgatc ggcttcttac
      421 aagcttatct tcagtacatc aatcagattt ctgaaccaat ttctaccgct tcttatgtga
      481 tcaactcgat ccaatccgca atggcagcaa tcgataggat atttgagata ttagatgaaa
      541 cagatgaaat tccagacctt ccagatgcaa gacttttaga acaaccgaaa ggaaaaatag
      601 ctttcaaaaa tgtacagttt ggttacacat cagataaaat attgatgaat caagtcgatt
      661 tttcagtaaa gccaaaacaa acggtagcga tcgttggtcc aactggtgca ggaaaaacga
      721 ctttagtcaa tctgctgatg cgtttttatg aactgaataa aggaaagatc gaattcgacg
      781 gtcttgatat taccaagctt tcacgtggag aactacgtcg ttcttttggg atggttttac
      841 aaaatacctg gctgtttgaa ggaacggtag ctgataatat cgcctacggg aaaatggatg
      901 ccacaagaga acaaattatc gaagcggctg aaatcgcaca atgtgatcac tttattcgaa
      961 cactgccaga cggatatgat acgatcattt ctagcgaaaa cggcacgtta tctcaaggac
     1021 agcaacaatt attgacaatt gctcgagtca ttctagcgaa tcctcctgtc gttattttag
     1081 atgaagcaac ttctagcgta gatacacgaa cagaagcatt gattcagcaa gcaatggaag
     1141 ctgtgacaaa agataggaca agttttgtga ttgcacaccg tttgtcaacg attgaaaatg
     1201 cagacatgat ccttgtaatg aagaatggcg atatcgtcga aaaaggaaca cattctgaac
     1261 tactccaatc accaacgctt tatgccagtc tttataatag tcaatttcag atagcgtaac
     1321 aaacattatc taaaaagcta tcgatcatgt atgattagtc catgatcgat agctttttta
     1381 aatgtactag tggtgccact agaaacacaa agaataatac taatgatact ttttggatag
     1441 ctccttcagt ttcgttcggt aagtagtgac aaaagacttg tcacaatccc tttttacgaa
     1501 taaacggtgt tcaagagaca acctatcggc aataagacac gatcttgtaa aatacgagaa
     1561 gctatacttc aaaatcgctt cttgttgctc ttgtcaactc cgaatcacga gttacactat
     1621 tcaacaaata tgagaagctg ttttttgaat ttgtttcttg tgactcggtg ctgaacgtca
     1681 ggctcacaat ctctcttctt taatctcctt gttctacttg cgatagttgc tgaacgaatt
     1741 gccatgtttc cccgttatca gttgaacgga agaggcaggc aaacattttc cctgcatcga
     1801 ttggtccttt tttcaacaaa taaaattttg tctctaacgt attcgcgctt atttgagtaa
     1861 cttcatttgg tgattgaaaa agatctatcc cccctgtctg atagttttcc ggaatcgaca
     1921 gaactgattc tttgaatgag cgtcctccgt tgtttgtata aaacaaactt tcacgtgtcg
     1981 aaacaaaacc taaagaagtt gtaacgaagc tgacattttg tactggctga ttgatcattg
     2041 tacttccact ttttatccaa gtgattccgc catccatagt agtgtaaatg tacaaagttt
     2101 ctgcagacat cccactggct gatgtacaga taaatagacc atcgttgcca taaaataccg
     2161 cttttcgata acgtatcggc tgaactgatt gggtgataat agacgtttgc catgttttac
     2221 cattatctct tgaagtcagc agacgtacat catccccaga agaatagata aaccatgaga
     2281 aatcggcgga aatatcatat gtgttgtcca tccaataacc ttcaggaatc agccccgttg
     2341 tcagggtgta ttcgccaaaa cgcaaccatt gcgaatcaat cggcacaaat acccaattag
     2401 caccactatc tgttgtcata tagagagagt gcgtctctgt atcaagtttg agatcattta
     2461 tcttttgtgt tgtcaaattt accttttgct taggatcagc taacaaccct tcatttgtct
     2521 tttgtatatg cgtatcgtta gttgagttca ttgtcatgtg agaagcagca tttatatgag
     2581 aagacatttt tggctgagtg tatctcccat aatagattgt tccagctaaa agaatgagga
     2641 aaaaaccagt gactaatgta ctcatcttag ttgtcaggta tccaccaggc gaagactggc
     2701 ttttttccca tctgggttcg ttttctagta aaaaaatatg gaatgtgttc attccgaatt
     2761 ttgatttgct ttcgcctaag tagataggta aatcatcaag tgatctcagc aataactggc
     2821 aatcactttt ttgtatccga aaagcttgac tcgtcgaagt cttctcaaaa taaagatagt
     2881 catcatcttg agaagttggt ttattatgta aaaactgttg tgctctttga ctaagccatt
     2941 gatcgaaatg acgcgacaat tgatggtaac ggagccaaat aagtagaaga aaaacaataa
     3001 gcacaacaat tgttattccg ccaactcccc acgaaaagtg atacaaatta tttaattttt
     3061 gcatcccttc agctgaacgg actactgcac tcatatggcg atataggaac gatacagctc
     3121 cgaaaaaaag caaaccagag aaaaatacta agcttaaaag cttgaacatt attcttttcg
     3181 ctcttttttt cgctttctcg ataatttttt gtttagctga taatacttcg ataataaata
     3241 gttcttgatc catttgtctc tccttttctc atacttcatt cattatgcgt caaaatccat
     3301 acaatcacta gtcccactaa taaaaaattt atgaatttgg tatgtactct caatttcagg
     3361 tagtttatac atcaaaaaat actaagat
//
LOCUS       DBKOIJ010000218         3350 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000218 DBKOIJ010000000
VERSION     DBKOIJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.225"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            90..1790
                     /locus_tag="ACV5NJ_002712"
     CDS             90..1790
                     /locus_tag="ACV5NJ_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HHY6486734.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1760..1978
                     /locus_tag="ACV5NJ_002713"
     CDS             1760..1978
                     /locus_tag="ACV5NJ_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HHY6486735.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1998..2888
                     /locus_tag="ACV5NJ_002714"
     CDS             1998..2888
                     /locus_tag="ACV5NJ_002714"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HHY6486736.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2906..3190
                     /locus_tag="ACV5NJ_002715"
     CDS             2906..3190
                     /locus_tag="ACV5NJ_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486737.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1187 a          551 c          713 g          899 t
ORIGIN      
        1 ttgttttctt atggaatcta caaagtcgaa tacgagactc atgacctttt tatgacgtac
       61 tggtatactt gaagaaaaag gagagcgcga tggagataaa aaaagtttac ttttcacaga
      121 aaatgtcgct gtattcgcct atccagcaag caaaacaacc ccttccattt tttcaaagtc
      181 atcaagaaag tagagacgct ttttggcgtt ctgcaaaaaa gcaatgtgct tttaaagcag
      241 tatcgaaaaa atataccgtc tattttacta ttgctaaaag tagaggagat gttattttta
      301 ataatctgtt gattgaaggt gaaacttttg aatggaaaaa attttttcgg tatatggaag
      361 cttgcgctcg tttttttatt aaagaaaact gttgtttttt atttcaatct cctctatcag
      421 aagaatggtt gcgtattttt cataaaaacg gctatcaagg aaccacacaa ttaaataaaa
      481 agctggtcta tcatacagca cttgttttag gtggaggagg agcgcacggt gcttatcaga
      541 ttggtgtgtg gcaggcgctg aaggaacatg atatcaactt tgagattatc acaggtacct
      601 cggtaggtgc gttgaacggc gcattgattt tacaaggaga tatgaaaaaa gctgtgaatt
      661 tatggaaaaa gctgtcgaca agacaagtgc ttgcgttacc agaaatggct gcagttgaag
      721 accttcgtga acgcttctat cgagaaagac gccaaatgac gaaaacagca ctaattgaag
      781 gcggggtttc ttctgctcca ttggaaaaga tggtcaaaga caatttggat ttgtccttac
      841 tcaaacataa tcccaaaata cgtttatata cggtgtctac taaactgcca gaactagcgg
      901 aagtagtgac tgatatccaa cagacaaaaa cagaagaaat accagattgg atactcgcct
      961 ctgcttcatt ttatcccgct atggcttatc gaaagatcgg aaaaaataaa tatgcagacg
     1021 gtgggtatcg caacaaaata cctattgata ttgcgattaa caaaggagca acagaggcat
     1081 ttgtcgtaga tgtgcaaggg ccaggcccag cgaaaaaaat tcgtatgcca gacattttta
     1141 tccattggaa atgtcaaaca ttgtggacac taggcagctt tctgcttttt gatagccaaa
     1201 ggaaccaatt aaatttacaa ttaggctatc tggaaatgaa aaaacgatta ggctattact
     1261 atgggaactg gtatacattt cattctgtta aacctgcaag aacatactgg cgaggatttt
     1321 tgtcttactt aacaaaagaa attcagttag aattatcttt tttcaaaagc ataaaatttt
     1381 ggcaaaaact gcgtaattta tataagaaca gggtcgtacc agagacatgc ggattggcga
     1441 tgctagaact tttagcaaaa aaacattttt tactgcccaa tgaaatctat caagtagaca
     1501 tgatgattca aatgatctgc caacaagata taaaaagtat gccggatgat ttattggcac
     1561 aaattggtca attaagtact gaagaatggc gaaggtatcg aaaataccag caaaaaatac
     1621 aaaatgagcg cacgaaaact gtttattttg attatctgct acaaggaaaa cgcaaggatc
     1681 gtttgcagca tgaattgctt aagcaaccag ttgacacatt actgatccta tatttatatt
     1741 atctcaagga ggaacaacca tggcacaaga attttcatat gaaatcgtag aagaaatcgc
     1801 tatattgtca gaaaacaaca aggggtggcg taaagaactg aacctagtca gctggaatgg
     1861 tcgcccgcca aaattcgatc ttcgagattg ggcgccagac catgaaaaaa tggggaaagg
     1921 cctgacattg acgaacgaag agtttgaaca actgcaaaaa gcaatagaaa atatgtaatg
     1981 aggaaggacc ctgtcgaatg aaaagtatga ctggttttgg caaagccatc cgcgaaacaa
     2041 aagcgtatca gttagatatt gaaataaaaa gtgtcaacca gcgttttcta gatgttcaaa
     2101 tacgaagtcc taaaatcctg aactttgttg aaaacgattt tcgtcagctt attaaacagc
     2161 agttaagccg cggtcgaatc gaagtatttg tgaatctagc ttatattggc aaaaacgaaa
     2221 aacagatcgt catcaactgg gaattgattg attcacttat cactcaattg acagacggaa
     2281 ttcaacaacg atacggtagt caaaatcaag tgtcaattag tcggatggcg gaacgcttcg
     2341 cgctggacga aaacttcgta acggtagaag aaaaaccgga agagagcatg gaagaattgg
     2401 cacagttagc tctagataca ttgaaaagcg cacttgattc tattgaggcg agtcgagaaa
     2461 aagaaggtca agctttagct ttagtgttga aaaagaatac tgaagaattc aaagaagtac
     2521 taaaaaaact cgatagtttt gtagaaatct atgaaaagga atttcaaata aggtatcaaa
     2581 aaaagctgga ggaatttctt ggagcgaaag tcgatgagca acggttgttg acggagctag
     2641 ccatcttgtt ggaacgcgga gatattcacg aggaactgga tcgtctggcg atccatgtac
     2701 aaaaactaga tgaacttatc gaagcacaat gtcctgtagg tcgggaatta gatttcctga
     2761 tccaagaaat gaatcgggaa atcaacacga tcggttccaa atcaaatgca atcgaaatta
     2821 aaaatcaagt cgtccagtct aagacgatct tagaaaaaat cagagaacaa gtacaaaata
     2881 ttgaatgacg aaagaggtag agataatgaa atattatgac gtaacgttcc acgaattaag
     2941 tggaaaaact gtggttaaac gggatatccc ttctgaaaaa aacggctttg acgtatggaa
     3001 agatgcctgt gcagactata atgagaacga actgtttatt ttgatcaacg acggtgctta
     3061 cgtgacgatg aatcggaaat ttattgttcg aattgacaca gaagaggtag aagatccgac
     3121 agaaaaagca cgttctcgaa aagatgagat catgggtgta gtgaatactt tgtccaatat
     3181 gggcttttag caaggatgct gaacaaagaa atacaataaa taatcagaat tggatttctt
     3241 cgcttagctt ctttgctcta ggaatacatt taaaggttgt gacaaaattc gtgtcacaat
     3301 ctttttttat tttgaataag tgatgttcaa aaaccatttt ctcggtatta
//
LOCUS       DBKOIJ010000219         3288 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000219 DBKOIJ010000000
VERSION     DBKOIJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.226"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACV5NJ_002716"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACV5NJ_002716"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HHY6486738.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACV5NJ_002717"
     CDS             complement(2260..2511)
                     /locus_tag="ACV5NJ_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HHY6486739.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACV5NJ_002718"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACV5NJ_002718"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HHY6486740.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          896 a          725 c          614 g         1053 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctac
//
LOCUS       DBKOIJ010000220         3200 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000220 DBKOIJ010000000
VERSION     DBKOIJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.228"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            301..585
                     /locus_tag="ACV5NJ_002719"
     CDS             301..585
                     /locus_tag="ACV5NJ_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486741.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACV5NJ_002720"
     CDS             588..1004
                     /locus_tag="ACV5NJ_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486742.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACV5NJ_002721"
     CDS             complement(1141..1860)
                     /locus_tag="ACV5NJ_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HHY6486743.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACV5NJ_002722"
     CDS             2522..2608
                     /locus_tag="ACV5NJ_002722"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HHY6486744.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACV5NJ_002723"
     CDS             complement(2911..>3200)
                     /locus_tag="ACV5NJ_002723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486745.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DBKOIJ010000221         3196 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000221 DBKOIJ010000000
VERSION     DBKOIJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.229"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(552..2165)
                     /locus_tag="ACV5NJ_002724"
     CDS             complement(552..2165)
                     /locus_tag="ACV5NJ_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HHY6486746.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..>3196)
                     /locus_tag="ACV5NJ_002725"
     CDS             complement(2306..>3196)
                     /locus_tag="ACV5NJ_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHY6486747.1"
                     /translation="NNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANLYAEFAH
                     KSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEIT
                     SIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLS
                     SLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQI
                     FFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHF
                     SSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1029 a          548 c          569 g         1050 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtt
//
LOCUS       DBKOIJ010000222         3151 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000222 DBKOIJ010000000
VERSION     DBKOIJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.230"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..393
                     /locus_tag="ACV5NJ_002726"
     CDS             <1..393
                     /locus_tag="ACV5NJ_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HHY6486748.1"
                     /translation="NFRLVLMGVGIGNAEFTNFFVSEFYKSFGNLHYQYFHHIWLFIE
                     VGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGPL
                     IYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            390..1109
                     /locus_tag="ACV5NJ_002727"
     CDS             390..1109
                     /locus_tag="ACV5NJ_002727"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HHY6486749.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1124..2209
                     /locus_tag="ACV5NJ_002728"
     CDS             1124..2209
                     /locus_tag="ACV5NJ_002728"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HHY6486750.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2345..>3151
                     /locus_tag="ACV5NJ_002729"
     CDS             2345..>3151
                     /locus_tag="ACV5NJ_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HHY6486751.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYY"
BASE COUNT         1118 a          348 c          602 g         1083 t
ORIGIN      
        1 aattttagat tagtcctaat gggagtaggc attggcaatg cagagtttac caattttttt
       61 gtcagtgagt tttataaatc gtttggtaat cttcattatc agtattttca tcatatttgg
      121 ttatttatag aagttggaat attaggagtt tcgatattcc tattttttat aataaagaca
      181 tttttcaatg caaaaagatc ttttatgaag gataaagatg caagtcttgt taaagttatt
      241 gcaattatgt cggtattctt ttttgtgtat aacacaacac ttaggggtga aacaacgggg
      301 ccactgattt atatgatttt agccatgcct gaagttgtga aaaggaaaga gcattattat
      361 agtgaaaaaa atacaaaagg agagatatta tgattcctaa gaagatacat tactgttggt
      421 tcgggggaaa tccacttccg aaaaaagata gaaagtgcat agaaagctgg aaaaagtatt
      481 gccctgatta tgagattatt gaatggaatg aatcaaatta tgatttaaat aagaaccgtt
      541 atatggccga tgcatataaa gaacgtaaat ggggttttgt tccagattat gcaagatttg
      601 atattattta taatcatggt ggattttatt tagatacaga tgttgagcta attaagtcgt
      661 tagatatact tcgtgaaaat catgcctata tgggatttga aggggaaatc tgggttaatg
      721 gcggtattgg ttttggtgca gaagaaggac attctgttat taaaggttta agggatattt
      781 acgattcatt aagtttttat caaaatgatg gtacattgaa cttgaaaccg agtccgtatt
      841 atattacaga ttatcttgaa tctattggat tggttcgcga taacagcgaa caaataataa
      901 atggcctgca tatctatcct acggattatt ttgctgccaa ggattatgat actggaaaaa
      961 tttataaaac tagtaataca ataagcattc atcaatataa tgcatcttgg aaaacaccta
     1021 aacaaaaatt tgtttataga ttaaaatcaa caattggaca aaaaaaattt aacaggttac
     1081 ttgcactgaa aaaattttta cacttataaa ggagaaaagt actttgaaga aaatttgttt
     1141 cattagtggt gtaatttctc gttctggggg aactgaaaga gtagggagta ttattgccaa
     1201 tagcttgtct gatttaggtt atgatgtcac tattttaagc ttttggaata gtggagagcc
     1261 ttattttggg ttagataaga cgattaaggt tgattatatg ttaaatccta aaacggaggg
     1321 aaaattatat agaacttatt tatatccggt ttggaaattg cacaagtata tatgcaaaaa
     1381 tgaatttgat atagtaattg atattgatac tttgttgtct ttgtatacta caagggcaat
     1441 taaaggaaca aagtgcaaat tgatttcttg ggaacatttt aattactgga caatggaaat
     1501 gctgaaagaa aagaagagat tcaaggctaa aaaattgata aaaaaatatg caaagaggct
     1561 tgtggttctc acagaagaag atcgtcaaaa acatattgat gaatatagtt tatcagaaga
     1621 ttttgttata actatgccaa atccgtgtat atctaatatc gagtacgaat atcattatga
     1681 taaaaagaga tttcttgcag tagggcgatt agtggaagag aaaaattttg ctgacatgat
     1741 aagtgcttgg aaaattgtag aaaaaaaagt agatgattgg gatcttatca ttgtaggaaa
     1801 aggtgaatta gaaaaagatc ttcgccagca gataaaagat ttaaatctgc aaagagtaac
     1861 tatagcaggg cattcagacc atgtggagga atattataaa tctgcatctt gttatgtgct
     1921 tagttctgtt tatgaaggat ttcctatggt tatattagaa gcacagtctt atggattacc
     1981 agttatttct tatgattgta aaactggtcc gagagatttg gtacatcata attttaatgg
     2041 gatgttggtt gaagataaga atatagacca attagctaag agcatgataa tgtttactaa
     2101 gaatactgat ttagcaatga gaatgtcact gaacgcatat aataatgtac aaaaatttaa
     2161 tttgaaagaa attacaaaac aatgggttgc tttgattgaa aatgtatagt tttttgtggt
     2221 tgtccaaaca tgaaaattag cgtaattaat caaagagcta gaagaaacct attacatttc
     2281 aagattttag aaattatata gtattaggta acaataaaaa atagaaagtt tggagccata
     2341 tgatatgata attaatcgta aacaaaatgc taaaaggaat ataattactg gaacaatttt
     2401 taagttatat cagattatca ttcccttttt tatgcgtaca cttatgattt atcagatggg
     2461 aatggagtat gctggattga acaacctgtt cacatctata tttcaaatgt taaatttagc
     2521 ggaattggga ataggtgctg cactaactgt aagtatgtat aaacctatag cagaagacaa
     2581 tagaaaagag atttgtgctt tactgaacgc atataaaaag tacttttcaa ttattggatg
     2641 gatagtgttt acattaggat tgttatgcat tccttttttg agatttttag ttaaaggttc
     2701 gattcctgga aatttaaata tgacagtttt gtatttaatg tatttgataa atactgtttt
     2761 gtcgtattgg ttattttcat atagaaagag tctaatatat gcacatcaaa gaaatgatgt
     2821 aattaacgtg tctatgctaa ttacttttac attccaattt gtaatgcagg catatgttct
     2881 gattttctta aaaaattact atttgtattt attggcttct attttgggac aaatagttct
     2941 taacttaatg tgtgctgttg tttctcaaaa gatgtatcct gattatactc ctgttggtga
     3001 gttagagaaa gaaactattg acgatattat aaaaaaagta aaaggattag ttacaaacaa
     3061 ggtaggcggt acaatattac gatcttcaga ttcaattatt atttcatctt tcttaggtct
     3121 ttcagtactt gcgatttatc agaattatta c
//
LOCUS       DBKOIJ010000223         3036 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000223 DBKOIJ010000000
VERSION     DBKOIJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.231"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="ACV5NJ_002730"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="ACV5NJ_002730"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HHY6486752.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="ACV5NJ_002731"
     CDS             complement(1032..1952)
                     /locus_tag="ACV5NJ_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HHY6486753.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /locus_tag="ACV5NJ_002732"
     CDS             2065..2817
                     /locus_tag="ACV5NJ_002732"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HHY6486754.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          855 a          612 c          526 g         1043 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3001 catatttttc gtttggttgg gcttattacc gcagat
//
LOCUS       DBKOIJ010000224         3004 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000224 DBKOIJ010000000
VERSION     DBKOIJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.232"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..962)
                     /locus_tag="ACV5NJ_002733"
     CDS             complement(<1..962)
                     /locus_tag="ACV5NJ_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6486755.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            1134..1268
                     /locus_tag="ACV5NJ_002734"
     CDS             1134..1268
                     /locus_tag="ACV5NJ_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486756.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(1261..1896)
                     /locus_tag="ACV5NJ_002735"
     CDS             complement(1261..1896)
                     /locus_tag="ACV5NJ_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486757.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..2644)
                     /locus_tag="ACV5NJ_002736"
     CDS             complement(2009..2644)
                     /locus_tag="ACV5NJ_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HHY6486758.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(2678..>3004)
                     /locus_tag="ACV5NJ_002737"
     CDS             complement(2678..>3004)
                     /locus_tag="ACV5NJ_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HHY6486759.1"
                     /translation="KNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMF
                     TKKYWNEMENLKKYIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKK
                     KELLGL"
BASE COUNT          852 a          508 c          435 g         1209 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
     2581 caattaacat gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt
     2641 ccattaatag atcctctttc tcttttgata ttgaatatta taaaccaagc aattcttttt
     2701 tcttaaaatc gaattcttct tgattaataa tcccttcatc taacaactct ttatactttt
     2761 taatttcatc tgcacttgaa atttgtgttt ctttggaatt attatctaat atagattgtt
     2821 gcttctctat atattttttt aaattttcca tttcattcca atattttttt gtaaacataa
     2881 cggtgttttc atctttcgtg gccgctaata tacctccgcg actttcgttt ccgcctagca
     2941 aggtaaattg tatataacca ttactcatac ctggtttttt taattgtacg gctgatatat
     3001 tttt
//
LOCUS       DBKOIJ010000225         2991 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000225 DBKOIJ010000000
VERSION     DBKOIJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.233"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(191..1501)
                     /locus_tag="ACV5NJ_002738"
     CDS             complement(191..1501)
                     /locus_tag="ACV5NJ_002738"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HHY6486760.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1513..2871)
                     /locus_tag="ACV5NJ_002739"
     CDS             complement(1513..2871)
                     /locus_tag="ACV5NJ_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6486761.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
BASE COUNT          950 a          614 c          467 g          960 t
ORIGIN      
        1 atagaatcaa gcttcgcaag tggttctttt taacgactta gttatcttat cacgtctaaa
       61 aacatgtgtc aactcttttt tggaattttc ataaaatgtt tcaatgaagc gcgacaacgt
      121 tgtttaatat atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct
      181 tattataatt tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag
      241 ataaatcaca gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg
      301 cagttatttg gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta
      361 aatcaaaagc tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga
      421 tgtgtatgtc tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt
      481 gccaactttc tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc
      541 agtccattat ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga
      601 tattttcgat cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact
      661 gaaaaaaatc gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa
      721 tagttacacc gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc
      781 gattgactct tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac
      841 aggccaaatc acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg
      901 tacttggtcc atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag
      961 tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt
     1021 ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa
     1081 gtcccttttg atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct
     1141 cccaccaatg gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg
     1201 ccgagagaac attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat
     1261 cgatttctgg aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga
     1321 taagatgact cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa
     1381 atacaggtaa gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat
     1441 cttgttgagt gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca
     1501 atacgtttct ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt
     1561 tgaacgattc agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc
     1621 tggtaacaag tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc
     1681 acctgttgta gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc
     1741 acatgcttgt cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct
     1801 taagcctgtc ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa
     1861 attgaataaa ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt
     1921 tgttatatga aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac
     1981 gcttgcttcc tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac
     2041 tactgttccg taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc
     2101 attgaataaa taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc
     2161 aacaatgact aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc
     2221 agatactttt tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat
     2281 atatgcgaca aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga
     2341 aacgacttgt tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt
     2401 agttgatccg ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt
     2461 aggatttgag ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa
     2521 aatcccataa acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg
     2581 ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct
     2641 gatccaagga ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac
     2701 catcacatca gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc
     2761 ccctgaaacg ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa
     2821 aataaaacag ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc
     2881 ctttttttct tgtttttata ttcacaatgt tggcttttta ttgcattatc ttgacagata
     2941 aaaaacaaaa aataatctaa aaacattgat gcataagatt gataactttt t
//
LOCUS       DBKOIJ010000226         2966 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000226 DBKOIJ010000000
VERSION     DBKOIJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.234"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            234..1559
                     /locus_tag="ACV5NJ_002740"
     CDS             234..1559
                     /locus_tag="ACV5NJ_002740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HHY6486762.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACV5NJ_002741"
     CDS             1552..1902
                     /locus_tag="ACV5NJ_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486763.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1884..2069
                     /locus_tag="ACV5NJ_002742"
     CDS             1884..2069
                     /locus_tag="ACV5NJ_002742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486764.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT         1061 a          462 c          555 g          888 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttac
//
LOCUS       DBKOIJ010000227         2894 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000227 DBKOIJ010000000
VERSION     DBKOIJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.235"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            110..925
                     /locus_tag="ACV5NJ_002743"
     CDS             110..925
                     /locus_tag="ACV5NJ_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486765.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1051..1308
                     /locus_tag="ACV5NJ_002744"
     CDS             1051..1308
                     /locus_tag="ACV5NJ_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486766.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1560..2132)
                     /locus_tag="ACV5NJ_002745"
     CDS             complement(1560..2132)
                     /locus_tag="ACV5NJ_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6486767.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2148..2753)
                     /gene="fic"
                     /locus_tag="ACV5NJ_002746"
     CDS             complement(2148..2753)
                     /gene="fic"
                     /locus_tag="ACV5NJ_002746"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HHY6486768.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          941 a          528 c          451 g          974 t
ORIGIN      
        1 aattactatc tagtacgaaa ttaattacat aggataatat attaaggtgc tggactacaa
       61 gtaaaaaata ctgtatgatc atgacagatt caagaaagga gcaataagat tggttaagat
      121 agcaggactt tctaaaggtg gaaaaaccgt ttcacaactt tataaacaaa actacagaga
      181 ctggagacag ttcagaaaaa acattaagga ttcttacttt attctaccaa cagaacttga
      241 aaaatattta ccgaataaca aagctattaa tttatatctg tattactgtt ttaaggcaaa
      301 gaatgaaggc ggggattcct ggcattcggt caataccata gcagagtctc taggagtgac
      361 aactaaaact ataaacacct ggaataaaac cttagttgat ttaggtatga ttgcacgtat
      421 agacgataac catctcagta aatcgacaat tttacttcct ctctctagtt actataaatt
      481 agcccttgat agttctttaa aggatgttac cgagagcact gtccatgaaa ttgatggcaa
      541 cttggttgca gtttatcatt tgtttgaatg gagaaaaaat gaggctgatg aaaattataa
      601 cgttccttat aacacactct gttgtgtttt taaaagaaaa acacaaaaag atactatcta
      661 taaatttatc ttggtccaag atgagaaaat aagtaatttc gaattaagca ctccttctag
      721 taagatttat gacgatgcat atagatttga atgtcaagat ctagaagccc tcctttccgc
      781 aaataatcta aacaatatag aacatgaaaa ctttgttgta aacactagat ttaatttagt
      841 ttctgaaaag gactcagatt tgttagattt gctgatagag cttactaaaa atctagataa
      901 gaaagaaaac attactgtac tataactagc atcggtccat cattaagttt ggactattag
      961 aacacttgct attacagaaa aaggtatgct agccacgctt actatttatt ggtatcaaac
     1021 aagtatctta atttgaaagg aagatgattt atggctaatt tatatataga gaacaaagga
     1081 gacttgaatg ccattattac tttaatcgta gttatggtaa aatcaatagg taatatcaca
     1141 ataggaatgc tgtttattat attagcagca gtgctgattt ttatgatagt tatttggttc
     1201 ttaaatgaaa gatctaaaga caatcaacac cgtagggata tggagataaa atcaatagag
     1261 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
     1321 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
     1381 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
     1441 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
     1501 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
     1561 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
     1621 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1681 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1741 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1801 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1861 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1921 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1981 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     2041 ttcttctcta cgccgttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     2101 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     2161 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     2221 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     2281 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     2341 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     2401 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     2461 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     2521 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     2581 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     2641 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2701 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2761 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2821 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2881 tttgcacagt ctag
//
LOCUS       DBKOIJ010000228         2860 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000228 DBKOIJ010000000
VERSION     DBKOIJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.236"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..473
                     /locus_tag="ACV5NJ_002747"
     CDS             <1..473
                     /locus_tag="ACV5NJ_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHY6486769.1"
                     /translation="KIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHLIDV
                     IGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKYGRK
                     VYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            566..1728
                     /locus_tag="ACV5NJ_002748"
     CDS             join(566..842,842..1728)
                     /locus_tag="ACV5NJ_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHY6486770.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2008..>2860
                     /locus_tag="ACV5NJ_002749"
     CDS             2008..>2860
                     /locus_tag="ACV5NJ_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6486771.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELS"
BASE COUNT         1022 a          477 c          581 g          780 t
ORIGIN      
        1 ataaaattca gtttaatcaa gttatagaag aattcatcaa agtaaataat ttaatgaccc
       61 atattcaaaa acgaccaatc agaataacta atgatctaaa actcagtacg agtatgattc
      121 atttgattga tgtgattgga aattatcctg aaactagtat tacggaatta gctgatcgat
      181 taggcgttac caaaggagct gtatcccaac agattcccac tttagagaaa atgaatctta
      241 taagcatttc tcaaaaaaaa ggaaataaga aaactaaact tgtatcgtta acaaaatatg
      301 gtcgaaaggt gtacgaatca cataattatt tacatgaaga gctttatagc tcaataaatg
      361 aagcgttaga atcattctca ccagaccaaa tttcaaccat taaacagatt ctagaaaaag
      421 tatcttatac aatcaatgaa tatcaagtaa gtctgacaag aaaggaagat tgacatgaaa
      481 aaaatgattt ttgaatcggc tacactagac tagacagaaa aaataaggtg tgtaaaataa
      541 atgaaaacac actggaggaa tttttatgtc aagacgtcaa cgtagaacct attcaaaaga
      601 attcaaacaa caagtcgtcg atctttatct cgcaggaaag ccccgagctg aaatcattcg
      661 agattatgag ctgactacct cggcctttga taagtgggtc aagcaagcgc aaactactgg
      721 ctcgtttcag gaaaaagata acttgacccc tgaacaagta gaactaattg cattacgaaa
      781 gaaaaataaa cagcttgaaa tggagaatga cattttaaag caagcggcgc tgatattggg
      841 acgaaaagac aagtaattga taccaataag cacaaatact caatatcagc gatgtgtaaa
      901 gtgttaaaca tctcttgcca aacctattat tatcaagcca aaccagttga agatgaatca
      961 gaactggaag aactcgttca agaagaattt attcgcaatc gaaaagctta tggtaccagg
     1021 aagctgaagc agtgcttggc caatctaggt cttcaagtga gtcaacgcag aatcggccgt
     1081 atgatgaaac agcgtggctt gcgttcaaca tacacattgg ctcatttcaa attccattct
     1141 tctacttgta acgaagccaa gaccgccaat attctagacg gaaccttcct acaggaaaag
     1201 tcattagaag cgattgtaac cgaccttacc tatgttcgtg ttgggaagaa atggcagtat
     1261 atctgcctga ttcttgattt gtttaatcga gaaatcatcg gctattcatg cggggagaag
     1321 aaagatgcga cgttagtcaa ggaagctttt gcaaagattc cgtactcgtt gaataatgta
     1381 caactttttc acacggatcg tggaaaagaa ttcgataacc agacgattga cgaaatttta
     1441 tgggccttta atatcacgcg ttccttaagc aaaaaaggct gcccctatga taacgccgtt
     1501 gcagaatcca cctataaatc tgtcaaagta gaattcgttc atcaataccg ctttgagaca
     1561 ttggctaatt tgcgattaga gctatttgat tacgtccatt ggtggaatac catccggctg
     1621 cacggcacgt taggctatga gacaccgatc agttttcgac agcagagatt ggcgcagcga
     1681 atccttgata atgagctcgg atgtgatacc ggtggcgagg caccttaatt atttgaatga
     1741 gcttctgcct tagagaatca gattcgagga ggctcgttgt caagggcagt cggaacacca
     1801 gcagcaagct tacgcaatac cttataattt ttgtcaaaaa aagtgttgcc attccacata
     1861 tatctttaaa cagctattgc cagataaaaa taaatgatat tatatatata aatacttatc
     1921 cgaaaagggg aatgagagag tcaaggttct tggaatgtcg aatagtcgag taggtagatt
     1981 ttctatctac aaccttgttg tttaaaaatg aaaaaaattg gaattagaga agttgcgaaa
     2041 gaggcaaatg tttctcctac gactgtgtcg agggtattga atgatcgtgg atatttgagt
     2101 gaggaaacaa aaagaaaagt ttttgatgcc atgaaaaaat tagaatacta tcctaatgaa
     2161 ctggcgagag cactttttaa aaacgaaaca tataccgtag gtttgatttt tcctacaatt
     2221 actaatccat ttcacgctga gttaattcaa gatatcgagt tcgagctttc cagcagggga
     2281 tataaagtgt tgctgtgcaa tagcttaaac aatccaacaa aagaaaaaga gtacttgacg
     2341 atgttgagaa agaatcaggt tgatggaatt atagtaggaa cgcacaataa agatattgcg
     2401 gattatgata ttccagggct acctattgta gcgattgata gaagtcttgg tgaaaataca
     2461 gttacagtat cttgtgataa ttacaggggt ggggaattag ctactcaact acttgtaaat
     2521 catggctgtc agaatatact gtgtattcgg ggagatagta agataaagct acccgcgaat
     2581 agtaggactt tagcatataa agatgttttg aataaattca tgctggaacc gttagtccta
     2641 gaagtaccat ttgtaaaaga aatcaatgaa aaaaatgaaa taattaagac atatctggaa
     2701 actcatccaa atattgacgg ggtttttgca ggagatgatt tattggcttc tttggcgatt
     2761 aacaacttag gaagttcggg caaaagagtt ccaaaagagg ttaaagtgat tggatttgat
     2821 ggagctcaac aaacattgat ttataatcca gaattatcaa
//
LOCUS       DBKOIJ010000229         2828 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000229 DBKOIJ010000000
VERSION     DBKOIJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.237"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1714
                     /locus_tag="ACV5NJ_002750"
     CDS             <1..1714
                     /locus_tag="ACV5NJ_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486772.1"
                     /translation="LKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGD
                     LSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYV
                     LYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALT
                     IIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQ
                     IKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSI
                     IQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLF
                     QKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAA
                     KENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGES
                     WEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDV
                     NAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKK
                     NENK"
     gene            1701..>2828
                     /locus_tag="ACV5NJ_002751"
     CDS             1701..>2828
                     /locus_tag="ACV5NJ_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HHY6486773.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMT"
BASE COUNT          950 a          417 c          465 g          996 t
ORIGIN      
        1 attaaaaaag gagaacaaca aatctatgaa aataagtaaa aaagcatttc ttttagtagt
       61 tcttatctta cttagtactt tatattcagt aaattttatg agaaatgctc aagaaattta
      121 tacaacaggt gatttatctt ttcatctttc acgaatcaaa gggttatctt ctatttttga
      181 agggccaatc aactatacaa catttaacaa ttatggagat ggactgaact atttttatcc
      241 ttttcttaca ataattcctg cagtcgtttt ttatggaatt tcaaataact taatattaag
      301 ttatgttcta tatatttggt tattaaatat ttgtacgatt ctgattagtt tttattatgg
      361 agaaaagttt tttaaaaaga tagatgctgc ttttttattt agttgtttat atacttttgc
      421 aacttatcga acagttgata tctattatcg atccgcaatt gcagaagcaa tcgctcttac
      481 gattatccca gctgttttat attatgctta tggattgata ttcaatagag aaaaaaaagt
      541 catcgcttta tctgtcagta taagtctact tgtgtatact catgtcttaa ctacactatt
      601 gtgtagtgca ttgattgctt gtatgattat agttcgtttg tttttaagca agcaaataaa
      661 aaacgatata aaatttgtgg tcgtgaattt agtaaaggct gtttttttga cttgtgcatt
      721 aactagctat ttttggtatc ctatgttgca gcagatgaat tatcaagaaa tacacagtcc
      781 cttcaaaaca gatttgcagc tagaagcact ttctatttca gatagtatca ttcaagcgtt
      841 aaataacgat attacaacat acactatggg gttattagga ttggttgcgt tgatttttcc
      901 tttattcata ctgaaagaaa tggagaaaaa agaaaaaatc atctatgttg gcgtagtttc
      961 tacatgggta tgcgtaacta atttatttcc atggtttttg tttcaaaaaa caccgataag
     1021 cttactccag tttccttggc gtatcttagg tatccaaatc tttttttctt caatgatcat
     1081 aacgcttgtt tttatgaaaa agactatgaa taaaaaatat tcatggttaa tcattggcgc
     1141 tactgtggtg tttctactcg tcacgtctat agcagcaaaa gaaaattacg caaaagttat
     1201 ccaatcaaaa cacggtcata ttgttatcaa tgaagaaaca ctcccacgtt atacaacatc
     1261 agatatggga ggattatacg attacgcccc aacagaagcg ataaaagaaa ggcctcactt
     1321 agaaaaacat gaagtaaaag taggagaatc gtgggaaaaa gggaagtata aagctgcaga
     1381 taataacatt acttacacag tggcaagcaa caaaaagcaa aaagtgacag taccagtata
     1441 tgcttattta ggtacacagg taaaagtaaa cggtaaagtc gtaaatgact cttataaaaa
     1501 acatggctta gtgaatgtgg atgtaaatgc tggagaaaat aagatagaaa ttacaacaaa
     1561 atatactcca actgctctca tggctttatg gatcagtatt ctgacttatt gtttagtggt
     1621 gtattcaagt tttaaacgat ttgcacctaa taagttgaaa gattctcgat atgtgactaa
     1681 aaaggagata aacaagaaga atgaaaataa ataaaaaagt agtaatattg agtggattaa
     1741 ttatagttag tagtatatat tctttcatct ttttcaaaaa tacacagagt atttatacca
     1801 ctggagatct ttcttttcac ttatctcgaa tcaaggggtt atcgactatt tttaccagtc
     1861 cgattaatta tgagacattc aattatactg gatatggtgt aaattatttt tatccctttc
     1921 tgactttttt tccagctgta atgttatatt ggatgacaaa aaatttgatt gtaagctata
     1981 ttatttacgt ttggttattg aacttgtgta caacactcgt agcttatcat tatggcgagc
     2041 ggtttttaaa acaaaaaaaa gcggcatttt tatttagttg tctctatatt ttctctgctt
     2101 atcggacagt agacatctac tatcgttccg caattgccga agctatcgca ataacacttg
     2161 ttatccctgt attattttat gcttatcaaa tcatctctgg aaaagaagaa aagtatccgt
     2221 ctgtaaagct agcattgagt atgagtttgc tagtgtacag ccatgtacta tcaacattaa
     2281 tgagtacagc attaattatc atttttattt ttattcgttt ggtttctaaa ggtttcaaga
     2341 atgcagattt tattgctata tttaaaaaat tatttagtgc ggcaggaatg acattggttc
     2401 tgacgagtgc tttttggtat ccgatgtttg aacaaatgct ctaccaaaaa atcaacaaac
     2461 ctagcgtgac aaatttatat gctcatgcat ctaatgtgtt cgatagtctg actgaagcta
     2521 tgaacaacga cttaactact tactcaatgg gactagttgg tctgttatct ttatgtattc
     2581 ccctgatttt atttaaaaaa ttaacaaaca tagaaaaaaa gatttattat ggtacgtgtt
     2641 tgacttggct agctacaact agtttggtgc catggtatct tctgcaaaat acgccagcca
     2701 aattgttaca atttccttgg cggatcttaa gcttgcaaat aatattctct tctctgatat
     2761 taacgatgat atttttcaaa aatagaagat acaataagac aagagaacta ttttatttat
     2821 ttatgact
//
LOCUS       DBKOIJ010000230         2828 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000230 DBKOIJ010000000
VERSION     DBKOIJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.238"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..271)
                     /locus_tag="ACV5NJ_002752"
     CDS             complement(<1..271)
                     /locus_tag="ACV5NJ_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HHY6486774.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTP"
     gene            328..1455
                     /locus_tag="ACV5NJ_002753"
     CDS             328..1455
                     /locus_tag="ACV5NJ_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6486775.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1673..2815)
                     /locus_tag="ACV5NJ_002754"
     CDS             complement(1673..2815)
                     /locus_tag="ACV5NJ_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6486776.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          911 a          519 c          470 g          928 t
ORIGIN      
        1 ctggggtcca accttcagtt gatctttttt caatgtattc tttttcctca gcaggaaaaa
       61 tgatttcggt tctcccacaa cgtcgcttat tttctttgta ttgttcaaag tattcaagtg
      121 ctgttccacc acatttgaga aagttataga cattgtaaat tgtttggcgt ccacgtttaa
      181 gctgcttcgc aacgatagca accggagttt cttgatgaaa atatgcttct atcattacaa
      241 gctcgtttgg tgtaagatgg gtataagtca tttatgttca ctctccttgt atgctttagc
      301 gggtattaca attggagtgt aacataaatg gcttttttat ttgtctcgct taattataca
      361 aacggcgaag atatcaatga tgatggctca agaaaaaaag tggagaaagt tggcggagac
      421 acccgaccag aggccgaagc tgctttacga aaagttttat cagatattga cgaaacagga
      481 caatactttt taggtacgga tactcgagta aaacaatacc ttgatttttg gatggaggaa
      541 tacgttaaac taaatctaaa atacaatacc tatgaaaact accgatttac catcaaaaat
      601 catataaacg gttatttagg aaagaaaaaa cttacggatc tctcccctgc tcttttacaa
      661 aatttcatca atgctgaatt taaaaagggt tactcgaaga aaacaatgac tattactcac
      721 tctgtcctta agaatgcgct gaatatggcg gtttatcctt ggggattaat caagcaaaat
      781 cctatgctgt atgtaaaaat accaaaatat gaagcacgac caacgactaa aaaagatcta
      841 aaaatcattt ctcttgagga ctttgatcat atgctagaaa ttactcctga aggacatcct
      901 ttctatattc ctttgaatat tggattttat acgggaatgc gcgttggcga agtttgtggt
      961 ctgacgtggg atgatgtcga tttttcaaat ggaacaatta ctgtagagaa acaaatggta
     1021 aagaatgacg gcgcatgggt atatggtacg ccaaagacaa gcagttccaa tcgaacgatt
     1081 tttattggac aaaccttgct agcaattctg aaaaaacata agaaacaaca attagaaaat
     1141 cgaatgaagt atggaaagct ctacattgat tcaaatgcag tatgtacgaa ggaagacggt
     1201 gagctagtta cgccaagtgt agtgaaatgg aatactagga ggatatcgaa tgcactctcc
     1261 ctctctttta acttccattc tctcagacat actcatgcta cacttcttct cgaaaatggc
     1321 gcaaaaatga aagaaatctc tgaacgattg ggccacagca gaatttcaat tacgatggat
     1381 acttactcgc atgtaacaga taagatgaga aatgaaacgg tcgatatcat ggaaaatctt
     1441 agaaagaatt cttgattttt gccaccgaaa aaaaccaccg gtggcaaacc ggtggcaaat
     1501 ccatgtaaac atggtttatt ttttacactg ttttcaaaca aacccttgct atttcttcgg
     1561 ttcgtcctcg tccattttcg ataccgacag ctagcttata acccaaggta tatcaacata
     1621 aaaaaataaa aataggctat gacagcagcg tgacagcaaa aaagatggaa agttaaatat
     1681 caaacctcgt aatttcattt gcgagtgctt gatttgtttc tggatataag tgtccatatt
     1741 tgtccaaagt agttttaata gaggcatgac ctagacgctt agatattgca tagatatttt
     1801 tattatgttc aattaaaaag gcaacatgcg agtgtcttaa tccatgcata tgaattggtt
     1861 ttactcctgc aatttcagct agttttttta ttctgcttaa aatagttctt ggagatggcg
     1921 gtaatccatc aaaagaaaaa accaattcta tatttttccc aattgattgt tgagtttttt
     1981 tccatgtttt taaatcttca acggtttgtc tgtctaacga aattatccga atacttgatg
     2041 tattttttgt ttcagcaaat tcataatctt gtcttgtacg gatgtaaatt gacttattta
     2101 cgctacatct gccatttatt aagtcaaagt ctttccattg taacgctaag agctcctcac
     2161 tccttacacc tgtaacaaaa ataaaacgca tcaaacgttt ataaaattct tcataaaaat
     2221 ccccgtgata aacactatta tatactttct taaattcatc aactgtccaa aattctactt
     2281 gaggacgctg agtttttatt ttacctatag tatctacagg attttcttca ataatttcaa
     2341 gtacaattgc ccttttaaaa actactctca acttattgaa tatctgttta atataatttg
     2401 gtgataatgg ttgctcaatg ccctttttta caacatggat tttcgataaa aactgttgaa
     2461 attcctgtat atgaattggt cgaatcttta caacctgcat ttctccaaaa aaatccaatg
     2521 cacgttgtaa aatatgatct gttttcatat atgttttttc tacagttccc aacttatacc
     2581 aaggaaaaaa atactctcga taaaaatcat gaaacgttat tccttgcaga taaattcctt
     2641 gtgaatcaga aaaactttgt tttaactctt cgagtcccct tctagcttct ccttgagttt
     2701 tgaagcctct tctggtcact tgaattcttt ttccaaagcg atcttgtcct aaagttgcag
     2761 aaaaaaacca agtgcctctt tttctgtctt tataaacatt cgcaattttc ctcattttac
     2821 tattcctc
//
LOCUS       DBKOIJ010000231         2760 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000231 DBKOIJ010000000
VERSION     DBKOIJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.239"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            92..556
                     /locus_tag="ACV5NJ_002755"
     CDS             92..556
                     /locus_tag="ACV5NJ_002755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HHY6486777.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            570..1319
                     /locus_tag="ACV5NJ_002756"
     CDS             570..1319
                     /locus_tag="ACV5NJ_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HHY6486778.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1319..2149
                     /locus_tag="ACV5NJ_002757"
     CDS             1319..2149
                     /locus_tag="ACV5NJ_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HHY6486779.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            2149..>2760
                     /locus_tag="ACV5NJ_002758"
     CDS             2149..>2760
                     /locus_tag="ACV5NJ_002758"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6486780.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKY"
BASE COUNT          920 a          357 c          535 g          948 t
ORIGIN      
        1 agtatggttc atattaattc gcttttaaaa gatgtcagtt aaggtggcaa tttctattct
       61 ataaatttta aaaattgaag gaggaaatag aatgattaaa ttagtgcgtg tagaccacag
      121 attacttcat ggacaagtaa tattttcttg gactaaacag tatgatatta accatattat
      181 tgtggctgat aacaaagttc caggagatcc aatgtctgtg atggctttga caattgctaa
      241 acctgctgat tgcaaattag acattgttaa aattagtgat gttaaaggga ttatggaaac
      301 atccccaaat gataattaca tgattttaat taaaggacct aaggaagctt ttgaattagt
      361 tcaaaattta ccagaaatta aagaaattaa ttttggcggt gtgtctaaga aaagcgagtc
      421 caaacaatat ggaaaggcag tctttttaac tcctgaagaa ttagaagcca caaaaaaaat
      481 tcttgatcaa gaagtgaaaa tatttgtgca acaagtccca tcttcaagta ttgaaaatgt
      541 agattttagt aagtaagggg ggaaaataaa tgttattaaa agccatttta ttaggtttag
      601 ttggtgtggt agctgttatt gactccaggt taattggtcg acaaaatatt ggtagaccac
      661 taattttgag tacacttgcc ggacttgtgc taggtgactt gcatacaggg attgttcttg
      721 gggcatcttt agaattaatt tctatgggat tcgtctcaat tggggcggct ggacctccta
      781 atatgcaact tggtagtatt attgccactg cttttgcaat tttaactaat agtagtacag
      841 aaacagcctt aacaatagct atcccagtgg ctgtagctgg tgaatttatg agtattatca
      901 tgcgtatgat aattgctcaa tttggacatg ctgcagataa agccattgaa aataataaat
      961 ttagaaaagc tcaaattatt catatttatt ggtcatttat ttttaatgct tttgtttact
     1021 ttattccaat ctttttaaca gtatattttg gtgctgacgt tgtggcagat ttagtagata
     1081 aaattccaga aatgattaca gacgccttga ctgtctctgg aaatatgctt tccgcattag
     1141 ggtttgcaat gctattaagc acaatgttga gtaaaaaatt atatccttac tttatttttg
     1201 gattctttat tgttgcctat tcaggtcttt cactaattgg cgtgacaatt tttgcagctc
     1261 tgattgcctt tgtaatggat caagttaaat atggaaaaag agaagaggcg catggttaat
     1321 ggaaaatagt acagaaaaaa aacgtttaat cacaaaaaaa gatcttcata agatgtgtct
     1381 tcgttcatgg acaatcatgt gttcgtggaa ttatgaaaga caaatgcata tgggatttat
     1441 gtatgggatg gcgccaattt taaataaagt ttataaaaat gatgaagaga aaaaaagaga
     1501 agcttatact cgtcatatgg cattttttaa ctgtacgcct caaatgactc catttattat
     1561 gggactttgt gcttctatgg aagaacaaaa tgctcaagca gaagaaggaa actttaatac
     1621 tgaatctatc tccatgatta aaaccagttt aatgggtcct tttgctggaa ttggtgattc
     1681 atttttccaa ggtactttaa gaattattac atttgggatt ggattgtctt ttgctaagca
     1741 aggtagtatt ttaggaccga ttttagctgt tggccttttt gctatacctt ctattttagc
     1801 tgttttttac ggcggtaaat taggatatga gtctggcaat aaatacctta ctaaattgta
     1861 tcaagaaggt cttatggatc gagtaatgca atttgcttct atcattggtt tggctgtagt
     1921 aggtggaatg gtggctagca tggtgccaat tgtcacccct ttaaaattca ctacaggggg
     1981 aacagaactt gttttacaag acttacttga ttccattatt cctggattat taccacttgt
     2041 atgtactttg ggaatatata aattgattaa gaaaaatgtt agtacaaata tgctcttatt
     2101 aggttgtatt ttggcagggt ttgttttaag tataataggt atcttataat gattaaggct
     2161 ctaatttttg atatggacgg agttttaatt gatagcgaga ttatgtatca aaagtgggta
     2221 tttaattttt taaaacaaga aaatttaagc tatcctttgt attcttatca aaaaaagatt
     2281 ggaactacag caagtatttt tgatgatata gaaaagtaca atgagggatg taacagttta
     2341 gaattgaagg gaaaatttac taaatattgg aaatcgcaaa agattgatta cttggatatt
     2401 tttcctaata tactaattga acagcttgaa tggttaaaaa agaaaaaaat aaaaatggca
     2461 attgcttctt ccagtccaat ggatactata aatgaagttg ttaccacttg taaaatagag
     2521 tcatattttg attgtttaat tagcggaaga gatttgccag aaagtaaacc taatcctact
     2581 atattcctta aagcttcaga acagctcaga attccagttg aagaatgtct ggtgatagaa
     2641 gattcttata atggtataaa agctggtaaa agagcaaata tgaaggttct tgcaattaaa
     2701 gataaacgtt tttctcaaga tgtaagtcta gcagatgatg ttatttacga tattaaatat
//
LOCUS       DBKOIJ010000232         2732 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000232 DBKOIJ010000000
VERSION     DBKOIJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.240"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            406..>2732
                     /gene="esp"
                     /locus_tag="ACV5NJ_002759"
     CDS             406..>2732
                     /gene="esp"
                     /locus_tag="ACV5NJ_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HHY6486781.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          987 a          423 c          540 g          782 t
ORIGIN      
        1 cttcaaattc cgaagaagga atttgaagtc acctttttac ttatgtcaca gtctcattca
       61 gtaatgcggg ataaggctcc ttattccgtt atttgaatac tctgaagttt aatctaagaa
      121 agggtaatat tttgaaaaca tgcttcatcg catagaaatg aacttaattt cagaaagaat
      181 acatacatat ctttttatat gttttcagct gctgaatggt taaaaagcac aaatatccga
      241 aaaaaacatg aagaaatgta ctttttttgt tgtaatgttc atgaaattct ctaagtaaat
      301 tagttaacat aaggttagta agattcctat tctgtgaata atacgtatca gatttatctt
      361 ttattcttac taggaaagta attattaagg gagttgattt gaataatggt tagcaagaat
      421 aataagagag tatttctaga aaaaacaaag aaacgagtat tgaagtacag tatcaaaaaa
      481 ttaagtgttg gggttgcatc tgttttgatt ggtgttggtc ttgtcttggg aacaactgaa
      541 ctagttaaag cacaagatga aatttctcca agcagcccac ttgaaacagc gataagctcc
      601 gtacaagtag gtgacaaagt agcttcgggg aatacttttc aggagaatcc tggttatacg
      661 aaaaattata acttttcgga tttacaattt agtcctcaag aactaactgg agacacacta
      721 aaaggcaata caattggatt tgaagtttac ggaaaacata atattgcagc atcaactaaa
      781 aactgggaaa tccgtcttca attagatgaa cgattagcta aatatgtaga aaaaattcag
      841 gtcgatccaa agaaaggaat tggatcttct agacggacct ttgtaagaat taatgattcg
      901 ctgggtagac ctacaaatat ttggaaggtc aattatattc gtgcaagtga tggattattt
      961 gctggggcag aaacaaccga tacgcaaacc gctcctaatg gggtaattac gtttgaaaag
     1021 agcctagatg aaatttttaa agagataggc atagacaatc tcaagaccga tcgtttaatg
     1081 tatcgaatct acttggtaag tcatcaagac gatgataaaa ttgtaccggg tattgatagt
     1141 acgggctact ttttaacaga ttcagatgat ttctataata gcttagatgt atctgaaaat
     1201 aatcctgacc agtttaaaca tggatcagta agtgcaaaat atgagaaacc taatactcaa
     1261 acaaaagatg gttcaggatc cacaggtgct aatggagcta taatcttaga tcataagtta
     1321 acgaaaaatt ataatttctc atattcatct tccgcaaagg gcacgccttg gtatgctaat
     1381 tacaaaattg atgaacgatt agttccatat gtagcgggta tacagatgca catggttcag
     1441 gctgacaaag taacatatga tgtttcgttt gaatccggaa caaaagtagc tgatttagca
     1501 atcgagcgac gggaaggtca tgaaaattat ggcataggtt caataactaa taatgattta
     1561 actaaactta tcgattttgc taatgcaagt ccacgtccag tcgttattcg atatgtttta
     1621 caattgacta agccattaga tgaaatctta gaagatatga aagcaacagc acaagttgaa
     1681 gaaaataaac catttggtga agatttcatc tttgattctt ggttgtcgga tacgaataaa
     1741 aaattaatcc agaacactta tggaacaggt tattattatt tgcaagatat tgatggtgat
     1801 ggaaaccctg acgataaaga agagagcgga gacacgaatc catatatcgg gaaacctgaa
     1861 ttagaagaag tatatgatgt tgacacaaca gttaagggga aagtattcat ccacgagtta
     1921 gcgggaacag gtcacaaagc ccaacttgtt gataaagaag gtactgtatt agcagaaaaa
     1981 actatcgctc caaatgaaaa agatggggct ccaatttcag atactgtaga atttgaattt
     2041 acgggtgtag attcaagtaa attaatcgcg aaagatgaat taaaaatcca aatcgtttct
     2101 ccaggttttg ataaaccaga agaaggttca accgttatta aggaatcacc aaaagcggtt
     2161 gataaacaaa ccgtggtagt tggatttaaa ccagatgcta aagaatcaat tcggaataat
     2221 aaaaacttac ctgaagatgc agagtattca tggaaaacag agcctgatac ttctaacgtt
     2281 actgatagta cgaaaggtat tgtaactgtt aagatcggaa atcgaacttt cgacgtggat
     2341 gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg atgcaacata cgtacctata
     2401 acaacaactc cagaaacgac agttcaaagt ggaaaaccta catttgataa accagatgtt
     2461 cctttagcta aagatgcctt ttcaatttca gatgtctata ataaggactt cggtaatgca
     2521 agtgttgatg caaacactgg tattgttaca ttcactccag ctaaaggtgt aggagaatcg
     2581 gagccgatta ctggaacaat tcctattaaa attgtttacc aagatggttc tgtaggcacg
     2641 accgatttag cagtaactgt aagtaaagat atttatgaaa atccaggaga aaacattcct
     2701 gcaggctacc acaaagtaac cttcaccgca gg
//
LOCUS       DBKOIJ010000233         2711 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000233 DBKOIJ010000000
VERSION     DBKOIJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.241"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            171..515
                     /locus_tag="ACV5NJ_002760"
     CDS             171..515
                     /locus_tag="ACV5NJ_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HHY6486782.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ACV5NJ_002761"
                     /pseudo
     CDS             516..935
                     /locus_tag="ACV5NJ_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="ACV5NJ_002762"
     CDS             complement(1027..1377)
                     /locus_tag="ACV5NJ_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486783.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="ACV5NJ_002763"
     CDS             complement(1343..2674)
                     /locus_tag="ACV5NJ_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HHY6486784.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1003 a          491 c          522 g          695 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact t
//
LOCUS       DBKOIJ010000234         2609 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000234 DBKOIJ010000000
VERSION     DBKOIJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.242"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            61..1464
                     /locus_tag="ACV5NJ_002764"
     CDS             61..1464
                     /locus_tag="ACV5NJ_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HHY6486785.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1813..2445
                     /locus_tag="ACV5NJ_002765"
     CDS             1813..2445
                     /locus_tag="ACV5NJ_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HHY6486786.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          983 a          369 c          510 g          747 t
ORIGIN      
        1 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
       61 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
      121 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      181 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      241 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      301 agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgatt
      361 tcaagtaccg aatcttcaac aacttcaagt agtacaagtg aaataacttc ttcgaaagtg
      421 acaaaaccaa accatataga atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca
      481 aaaacttttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc acaaccaata
      541 ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa ttttattatg
      601 aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc ttctgttatg
      661 attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca ggcaccgaat
      721 tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat ggtaacaaac
      781 gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt gtatgaaaac
      841 tatgaggaaa gctttgaaga ttatgccaaa ttattaacta agggaattag tggaaataaa
      901 gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc aactaaattc
      961 cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga attaattaaa
     1021 acctacgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag taagactgga
     1081 taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg tggaaatgaa
     1141 tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc agttaaagct
     1201 ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt gatacaacac
     1261 gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa aactggagat
     1321 aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag tactggccca
     1381 caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt agttgatccg
     1441 aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg
     1501 gttggattct tagacctttg atcaggatat tgagactgga attgatatct cgattacgat
     1561 gggtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg ccgtctttat
     1621 gctcatattt tgagcaggtt tcactagatg aaaacaattt atctatcttg aggatttcgt
     1681 gtacgtatcg ttcagtcttg tattgtaaat tagtcataaa atcagactaa gaaaaattgg
     1741 aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt ctttcttggg
     1801 agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga gattactcat
     1861 aatggatata aaaaatatcg ttgcctatgt gactgtggga atgaaacgat tgtatatcga
     1921 tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg gaaattataa aaataggcat
     1981 aaatataaaa atttgacaaa taaaactttt ggtaagttag ttgcaatcaa gccaactaaa
     2041 ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta
     2101 gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa
     2161 aagcatcaca ttgaaaataa aaaattcgga ttacttactg caaagtatcc tttggacaat
     2221 aactgttcat ggaaaacaaa atgggtttgt cattgtgagt gtggcagtat gtgtgagata
     2281 agttatagta acttattttt tggacatact caaagttgtg gatgtttaaa aaggcaagaa
     2341 acaaggacat gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat
     2401 aatacaagtg gtgttaaggg agtttatcgt tctaaagaga aatagattgc gtatataact
     2461 ttaaaaaaaa agaggtacta tttaggatcg tatgattcta ttaagagtgc agctcaagct
     2521 agaaaagatg ctgaaaataa attgtttcaa cctttgattg acaaatatcg aggtaatcat
     2581 taattattgt ggataataat taacagtga
//
LOCUS       DBKOIJ010000235         2594 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000235 DBKOIJ010000000
VERSION     DBKOIJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.243"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1412
                     /locus_tag="ACV5NJ_002766"
     CDS             <1..1412
                     /locus_tag="ACV5NJ_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HHY6486787.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2594
                     /locus_tag="ACV5NJ_002767"
     CDS             1546..>2594
                     /locus_tag="ACV5NJ_002767"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HHY6486788.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARL"
BASE COUNT         1018 a          363 c          520 g          693 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tatt
//
LOCUS       DBKOIJ010000236         2526 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000236 DBKOIJ010000000
VERSION     DBKOIJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.244"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(90..1139)
                     /gene="recA"
                     /locus_tag="ACV5NJ_002768"
     CDS             complement(90..1139)
                     /gene="recA"
                     /locus_tag="ACV5NJ_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HHY6486789.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1248..2489)
                     /locus_tag="ACV5NJ_002769"
     CDS             complement(1248..2489)
                     /locus_tag="ACV5NJ_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HHY6486790.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT          652 a          582 c          454 g          838 t
ORIGIN      
        1 taactcacga tttctttatt caataaaaaa acggcaggtc aaggagagta cctactcttc
       61 agaccagccg tttttgttta tcataatttt tattcttcta ctaaaggcaa ttcttcttgt
      121 cctttttcat cttctgggac agcgacttct tcaccaatac cataagctgc tctaactaga
      181 gcagaaactt ctgccatcat ttcaggatga ttagccatat agattttagc gttttcccgt
      241 ccttgaccaa tccgatcttc tttataagaa taccaagcac cgcttttatc aacaatgtct
      301 ttttctactg ccatgtctag aagttctcct tcttgtgaga tccctaaacc atacatgaca
      361 tctacttcag cgattttgaa aggaggtgct actttgtttt ttacgacttt gatctttgta
      421 cggttaccaa caatatctgt accttgtttc aattgttctg cacgacgtac ttccaaacgg
      481 atcgttgcgt agaatttcaa tgcgcgtcct ccaggagtga tttctggatt cccaaacatc
      541 acgcctactt tttcacggat ttggttgatg aaaatagcga ttgtttttgt tttgttgatc
      601 gaaccagaga gtttacgcaa tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag
      661 tcacccattt cgccgtcgat ttctgctcgt ggaactaaag cagcaactga atcgactact
      721 acgatatcta cagccccact tgatactaaa gcatcagcga tctctagacc ttgttctcct
      781 gtgtcaggct gtgaaagaag tagttcatcg atattcacac ctaatttttg tgcatattgc
      841 ggatctaacg catgctcagc atcaatgaaa gcggccgttc cgccattttt ttgtacttct
      901 gcaatagcgt gtagtgcaac tgttgtttta cctgaacttt caggtccata tacttcgatg
      961 atacgtccac gggggtaacc gccgacacct aaagcgacat ctaacgcaag agagccactt
     1021 ggaattgtag agatttgttg gtcgattttt tcgcccaatt tcataataga gcctttacca
     1081 tagctctttt caattttttt cagtgcagca tctaaggctg ctttacgatc atctgccaat
     1141 acgataatcc tccttatatt tacaaatcag agcaacattt cattatcctt atcatatctt
     1201 ttttagtgaa aaaaagcaag aaaaaaccga acaaatattc gcatttatca ttttcttaaa
     1261 agctgttttc tcaaaagatc aaatccttgc ataaccgcat tatatctgat agcagcacga
     1321 tctctggcaa agtgatattt ttccgaaacc acgccgtctt tagatgaaat agcgatccat
     1381 actgttcctg cagattgacc ttctaacgtg tccggtccag ccactccggt gaaagctaca
     1441 ctgtaatctg ttcttgctat ctcacgtgct tgaatggcca tctgctccac acattctttg
     1501 ctgactgttc catattctgc taatagctcc ggatcaatct ccagaaaacc agcttttgtt
     1561 tggagcgagt aggtaacgaa tccgcctgga aatacttcag atacaccagc aatgtcgcct
     1621 aatgcggctt ggaaggcgcc ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt
     1681 tctttcagta gttccactac gacttttgct agcgaattat catcgccgta tccataaaaa
     1741 tattccccta ctcgttcttg gattttttct tctaatgcta aaagagcttg attccctgcc
     1801 tgaacatcat ttgttttcac tgtcagtcgt aatgtcactt catttggttt ggcatatggt
     1861 gcgatcgttg gattgatctg tgtctcaatc agatctttta gctcagtgac cagtcgcgat
     1921 tcaccaatcc cataaaagcg caaaacttta gaaattagtt tttcttcaga aggaaaacgt
     1981 tcctctagta aaggacgtac ttgttccaca aacattggtt tcaactcatt aggcggaccg
     2041 ggaagaagga tatacgcatg tgtatctgtt tgataaaaag tccctaaagc aagccctgta
     2101 cgatttggta gaggaacccc gccttcgata atctgggact gttgcagatt gtttttggtc
     2161 atttttcgat gggttgtttc aaaatatgcc aataattttt tgtatccttc cgtattttgt
     2221 atgagagact taccgaggtg agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt
     2281 cctaatccgc cacataagag tatgagttca cttcttgttt cagctagagc aatcaactct
     2341 tctaaacgct gctgattatc tcctacaact gtttgataat aaacttcgat acctagatca
     2401 gctagctgtt ctgacaagaa agtggcattc gtattcacga cttgtcctag taaaagttct
     2461 gttccgacag ctattatttc tgctttcatt acttcccgct cccttatata gtatacgctg
     2521 tatttt
//
LOCUS       DBKOIJ010000237         2461 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000237 DBKOIJ010000000
VERSION     DBKOIJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.245"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(181..747)
                     /locus_tag="ACV5NJ_002770"
     CDS             complement(181..747)
                     /locus_tag="ACV5NJ_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHY6486791.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(747..1703)
                     /locus_tag="ACV5NJ_002771"
     CDS             complement(747..1703)
                     /locus_tag="ACV5NJ_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HHY6486792.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1703..2359)
                     /locus_tag="ACV5NJ_002772"
     CDS             complement(1703..2359)
                     /locus_tag="ACV5NJ_002772"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HHY6486793.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          739 a          520 c          457 g          745 t
ORIGIN      
        1 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgat ttggttggac
       61 ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga aatcaacgct
      121 ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg cgttttttat
      181 ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt tgataaaccg
      241 ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat caaccatatc
      301 cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc gtccgcgagg
      361 cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca tagtgatgat
      421 tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga gattttgttg
      481 ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga cacgttcttc
      541 taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct ggatatcaaa
      601 actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat cgtggccatt
      661 gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca gtaatgtatg
      721 gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac atcatatttt
      781 ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac ttcccatttt
      841 ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt caaacgatgg
      901 atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac atagtcagga
      961 cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt tttgcgcatc
     1021 agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt gcgtctgaca
     1081 ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg cgtacaaaca
     1141 cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata atcatgtgct
     1201 cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc tacccataaa
     1261 tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag acaatccggt
     1321 cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc aaatcgttct
     1381 ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac gatgtattgt
     1441 tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg gattggaaga
     1501 gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa agtacaaccg
     1561 ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat cggcaccttg
     1621 aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata tcttttattt
     1681 ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta tctatttgtt
     1741 ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg agtaagtagc
     1801 cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc ccgacactta
     1861 ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt tgaacaaaag
     1921 ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca tgtccactag
     1981 ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct cgcatgaaaa
     2041 atagtttgat caccgggttg atgattccag caattacaat aaatccagaa acgaaccaaa
     2101 atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca aataaaatga
     2161 tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc agatcaggat
     2221 aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac gacagatgga
     2281 actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca gccacctgaa
     2341 atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta tagcattttt
     2401 ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct ttgaataacc
     2461 a
//
LOCUS       DBKOIJ010000238         2459 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000238 DBKOIJ010000000
VERSION     DBKOIJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.246"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            6..1150
                     /locus_tag="ACV5NJ_002773"
                     /pseudo
     CDS             6..1150
                     /locus_tag="ACV5NJ_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="ACV5NJ_002774"
     CDS             1321..1779
                     /locus_tag="ACV5NJ_002774"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HHY6486794.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACV5NJ_002775"
     CDS             1938..2408
                     /locus_tag="ACV5NJ_002775"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HHY6486795.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          460 g          716 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DBKOIJ010000239         2451 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000239 DBKOIJ010000000
VERSION     DBKOIJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.247"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            69..635
                     /locus_tag="ACV5NJ_002776"
     CDS             69..635
                     /locus_tag="ACV5NJ_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HHY6486796.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            780..867
                     /locus_tag="ACV5NJ_002777"
     tRNA            780..867
                     /locus_tag="ACV5NJ_002777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:812..814,aa:Ser,seq:cga)
     gene            1201..2175
                     /gene="bsh"
                     /locus_tag="ACV5NJ_002778"
     CDS             1201..2175
                     /gene="bsh"
                     /locus_tag="ACV5NJ_002778"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HHY6486797.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          840 a          371 c          474 g          766 t
ORIGIN      
        1 gcaaaaaatg ctaaaaatag ggtacaataa aacaacatat actgcagtaa agaagtgggg
       61 gagttttaat gagaagaaaa gtctatacca aggatcaaat tctaaaagct gcttatgatg
      121 ttgttgcaaa ggaagggttt aaaggattta ctgcgagaaa tattgctaaa aagatgggga
      181 tttcgacaca gccgatttat ctggaattca aaaacatgga agatttaaaa aatacgttgc
      241 tggattctat ctttgaacaa ctaaaagaag atgttttccc tattgaacgt actggtgata
      301 aaatcattga tctcggtttg aactatatct actttgcaaa aaagcatcat agtctttaca
      361 tggcattgta cattgatgaa catggggacg gaaagaaaat gcatgatttt tcatacaatt
      421 atttccgtga aatagttaaa gaagatgaaa gatacaatca attatcggat gaatatgtcg
      481 atgctctgca tactggtaca tgggtagcag tgactggctt agcgtcattg atgtcatcaa
      541 acatcatgca tccaacagaa gaacaaatca ttgcactgat caaacggaca attgactcga
      601 ttttagcatt agataatcct gaaataatca gatagtgttc tatcagtatg aatttgatcg
      661 gtaagtccgt tggctgcaaa ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt
      721 atttatctat tagaaatagg cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg
      781 gaaggttggc agagtggtaa tgcaccggac tcgaaatccg gcgaaccggc taataccggc
      841 gcgcaggttc aaatcctgta ccttccttat atcaaagatt agaggttgtg aagaagctgt
      901 cctgcatcaa gtaataagaa cggccaaaca aaaatagctt ttcctatttt ttcgtttgat
      961 tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaata aggggctgtg
     1021 acaagacttt tgtcacagcc ccttattgtt gtacttttaa cctacaatat tttttgattc
     1081 cccctgagta ctatttttta taaatgctag taaatcaatc ttctttggcc tttttttgat
     1141 aaaaaaaata ctagatgtaa gataaaagtg taaaaacgat ttgttttagg aggggaaacg
     1201 atgtgtaccg caatcactta tgcaacgaaa gaccattatt ttggaaggaa ttttgattat
     1261 gagatgtctt ataatgaagt ggtcactatt actccaagaa attatcgttt cgattttaga
     1321 aaggtaaaaa atttagataa acattatgca atgataggaa tagcagctgg ggtatccaac
     1381 tatccactct attatgaggc tacaaatgaa aaagggttga gtatggcagg actgaatttt
     1441 cctggcaatg ctgattataa agaattacaa gaaggaaaag ataacgttgc accatttgag
     1501 tttattccat ggatactagg gcaatgctca accatagacg aagctaaaga attattggct
     1561 actatcaatc tagtcaatat cgattttagt gaaaaactac ccttgtcacc tttacactgg
     1621 ttattagctg ataaagaaaa atcgatagta attgaaagta tgaaagatgg acttcattta
     1681 tatgataatc ctgtcggtgt gttgacaaat aatccgccat ttgattatca attatttaat
     1741 ctgaacaatt atcgctcgct atcaaatgga acaccagaaa atcatttttc aaaccagatc
     1801 agcttgaatg tttatagtcg tgggatggga ggattgggct tacctggaga tctctcttct
     1861 gtttctcgat ttgtaaaagc cacatttact aaaatgaatg ctgcatcagg tgattcagaa
     1921 tctgaaagta tcagtcaatt ttttcatata ctaggctcag tcgagcaaca aaaaggagta
     1981 tgcgatactg gagaaagaaa atacgaatat acgatttatt cttcttgctg taacgttgat
     2041 aaagggattt attattatcg tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat
     2101 aaagaagact tggatcgtca tgaattggtc agttatccag tcataaaaga gcaacagata
     2161 aactatgtca attaattaat gaatagatcc gtttggtatc atattttctg atacagtcat
     2221 aattagacac gatctttagg tcgtgtcttt ttttgctggt ttttttggaa atagcatcca
     2281 ctaatatttt tttaacacga agagaataag gtagcctcaa aaaagaaaac atggtaaatt
     2341 agatattggg actttatttt ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac
     2401 gaaattgtaa gagagaaaat attcgtccgc tatttattct atgataagat g
//
LOCUS       DBKOIJ010000240         2414 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000240 DBKOIJ010000000
VERSION     DBKOIJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.248"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            263..2407
                     /locus_tag="ACV5NJ_002779"
     CDS             263..2407
                     /locus_tag="ACV5NJ_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HHY6486798.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT          844 a          504 c          456 g          610 t
ORIGIN      
        1 caaactttgg tataatttac atatgcataa ccatttttat ccccacttgt ttatccccaa
       61 acaaaggtgg tctatgcaat aaaatgaacg tcaaagaaaa acgaatgaat gctcgttgct
      121 tatttacaaa ttgttttatt tagaatacac agacagattt atgaaaagaa aaagcttttc
      181 gacacgatta tctgttccaa aaaatgtcta atgaatgctg aataagacag aaaatcaatg
      241 aaaggaaaag gactagttta ttatgaaaaa taaaaaaacg atacgtttgc tagctgctac
      301 ttggctgatt gctccgtctg ttttgtcgac caataccgta tttgccgtag ataatcaaat
      361 agctgatcaa acagtcacaa cagatacgac tacatccagc tcagctacta gtgaaacaac
      421 ggattctttt acagaagacc caactggaac aactaactca acaacaggat cgaccgagcc
      481 aactgaaatt gatccggctg atccatcaga aaatgaaaag ccagccgaga catattccct
      541 tacaattgct ccttctttga ttggtaagat ccaactgact gcttcgacag cggattcccc
      601 tgaacagcaa cctttggata ttacggaaag tggaacggta gaaggtttat tagcggaaac
      661 gaaggtaact tacactatta cgccagctga aggtgaacaa attgcttcag tcgctgttaa
      721 tggtgccaaa gaagtaaatt atgatgcaaa gacctttaca gtaggtacga acaatttatc
      781 tattactgca gaatttaaag ctggaccgga tacttcaaca gagaaaccaa ctgatcctag
      841 tgaagaagtc cctagtacac cagatccgga aacgccaaac aaaccagatg tgggtaataa
      901 caacaataat aatggttcaa ataattcaaa caataataac aataattcga acagcgggaa
      961 ttcttcgaat caaacaaacc catctggatc gaataacaat ggaaatactc agagagaacc
     1021 aaaccatcca aacaatggtt catcgaatgg cggaacgaac aatgcttcat ctcgtccaaa
     1081 tacagcaaat caaagcggta tagagaatcc atcttctgct tcttctgatt ttgttgtaaa
     1141 atcaccaatt gatgctgtat tgcctacaaa tacaacgaat gtccaacaag caatcgttcg
     1201 agaagcattc aagcatcttg gaaaaccata tgtatgggga gcaaaaggtc caaatacttt
     1261 tgactgttct ggtttgactt attacgtata tatgaaagca accggtcatt atatcggtgg
     1321 ctggacaggc gaacagcaat atgcaggtac gcagattcca gtaagtcagg cccaacctgg
     1381 cgacctcgtc ttctggggac cttcttccgg tgttactcac catgtaggta tctatattgg
     1441 gaacggacaa tttatccatg cgcctcaacc aggagataaa gttcgagtaa cttctatttc
     1501 agatttcacg cctgattttg ctgtccgagt aaacttagca ggtttacctg ctgcttcagg
     1561 ttcgctgaca ggcggctcat ctattttaga tggattagat ggctcgttcc atttcagtca
     1621 aaaccaaaca actgatcagt tcttgaaaaa gattgcagat gatgcacagg aaatcgggca
     1681 aaaagaagga atctacgcat cagtcatgat ggctcaagct atccttgaaa gtggatctgg
     1741 aaacagctta ttatcaagcg aaccaaatca taaccttttt ggtatcaaag gatcatataa
     1801 aggaagcagc gtgaccttca atacacttga acaagatagt tccggacaaa gctaccaaat
     1861 ccgcgcacaa ttccgtaaat atccttctta caaggaaagt ttggaagact atgccgactt
     1921 gatcaaaaat ggactgactg gcaatcctga tttctacaaa ccaacttgga agtcggaaac
     1981 aaaagactac aaagaagcaa ccaaatattt ggaaggacgt tatgctacag atcgccaata
     2041 ttcacaaaaa ttgaacgcga tcatcgaagc gtacgacctg acaaaatatg atgaaccgaa
     2101 aaaagaagca gcaacaactg aagaagcaaa cgctgaaatc ctcaatatgt ctaagcgatt
     2161 cgatgtgcca atcaaatgga caacagatgg cgtgccaatc acaggattag aattttcaac
     2221 gataaaacgc gtcaacaacg cttcttccat cttccgtgtc gcttcaatct gggatttgtg
     2281 gaacaatttt acggcaagac agataccaga aacaactact cgtacagtta aagctgcaac
     2341 tgttcctgta ctaaccttgt taagtttaga caaatcattc tggcttgatc tgcctttaaa
     2401 gaaatagaat aaat
//
LOCUS       DBKOIJ010000241         2398 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000241 DBKOIJ010000000
VERSION     DBKOIJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.249"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            207..2069
                     /locus_tag="ACV5NJ_002780"
     CDS             207..2069
                     /locus_tag="ACV5NJ_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HHY6486799.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACV5NJ_002781"
     CDS             2069..>2398
                     /locus_tag="ACV5NJ_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HHY6486800.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DBKOIJ010000242         2395 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000242 DBKOIJ010000000
VERSION     DBKOIJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.250"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            316..570
                     /locus_tag="ACV5NJ_002782"
     CDS             316..570
                     /locus_tag="ACV5NJ_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HHY6486801.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACV5NJ_002783"
     CDS             571..888
                     /locus_tag="ACV5NJ_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HHY6486802.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACV5NJ_002784"
     CDS             1447..2382
                     /locus_tag="ACV5NJ_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486803.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DBKOIJ010000243         2389 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000243 DBKOIJ010000000
VERSION     DBKOIJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.251"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(261..764)
                     /locus_tag="ACV5NJ_002785"
     CDS             complement(261..764)
                     /locus_tag="ACV5NJ_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HHY6486804.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1123..2148
                     /locus_tag="ACV5NJ_002786"
     CDS             1123..2148
                     /locus_tag="ACV5NJ_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HHY6486805.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          746 a          377 c          447 g          819 t
ORIGIN      
        1 aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg ttaagatttt
       61 aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag cctgtcagtt
      121 tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa atagattttt
      181 ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt gtctattacg
      241 gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa taaaaaagct
      301 acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc taacttattt
      361 gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc tcctgttgta
      421 ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg aatcaattct
      481 gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag tctcttgaaa
      541 ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt ggtctcacgt
      601 aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac attgctgtaa
      661 gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa ccataataga
      721 aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat ctcctccatt
      781 taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag tttaaggacc
      841 aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg tttctttaac
      901 aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg
      961 gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg
     1021 aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc
     1081 ttggttcagc tttttttcca attactagga aggagggata gattgttcaa gacttatttt
     1141 cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt
     1201 attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta
     1261 gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg
     1321 tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt
     1381 ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga
     1441 ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg
     1501 agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc
     1561 gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca
     1621 attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct
     1681 gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat
     1741 aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg
     1801 atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca
     1861 ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat
     1921 ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg
     1981 atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat
     2041 cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg
     2101 gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat
     2161 atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa
     2221 agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa
     2281 aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct
     2341 gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcat
//
LOCUS       DBKOIJ010000244         2387 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000244 DBKOIJ010000000
VERSION     DBKOIJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.252"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(4..249)
                     /locus_tag="ACV5NJ_002787"
     CDS             complement(4..249)
                     /locus_tag="ACV5NJ_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486806.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ACV5NJ_002788"
     CDS             complement(266..1117)
                     /locus_tag="ACV5NJ_002788"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HHY6486807.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ACV5NJ_002789"
     CDS             1283..2287
                     /locus_tag="ACV5NJ_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HHY6486808.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          762 a          403 c          482 g          740 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtg
//
LOCUS       DBKOIJ010000245         2369 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000245 DBKOIJ010000000
VERSION     DBKOIJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.253"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(245..613)
                     /locus_tag="ACV5NJ_002790"
     CDS             complement(245..613)
                     /locus_tag="ACV5NJ_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HHY6486809.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            763..1245
                     /locus_tag="ACV5NJ_002791"
     CDS             763..1245
                     /locus_tag="ACV5NJ_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HHY6486810.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1282..2292
                     /locus_tag="ACV5NJ_002792"
     CDS             1282..2292
                     /locus_tag="ACV5NJ_002792"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HHY6486811.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          835 a          364 c          471 g          699 t
ORIGIN      
        1 gtattttttc cttattcaaa agttttgcag gaaaattgct gaaaaaataa ttttactaag
       61 tagaaaattc aaagccattc aatcagtgat ttttttcagc acaagaaaga ctccttataa
      121 aaatagcaga tactttgtca attaagtgtt taaccaggga tgataaaaag gataggattg
      181 aacaaaacct gtataaggtg atgaatagtt tcctcgccag taaaaggttt attcgtataa
      241 agctttatcg ccaaaattta tcatgacctt ttttatcttc aatattaaat gaaatcaatt
      301 gttcaatcaa ttcccagttg attacacttt tccaaggaac tttgatgacg ttatccgtat
      361 atacgtagcc tgcttcttgg atattttttt caagggcacg aatcgctgct ttttccggtg
      421 cgatagaaaa atgattttta gcaaaactaa atgcaataat aaaggtacca tggtcagtaa
      481 acataggttg gttccatttt acagttgtgt ctagctgggg atacgtttct gttatcttag
      541 caaaaaggtc tttgatcttt tgttgatgtt ctttttcttt gatttgattg atgatcggct
      601 gaaatttttc cattctaaaa cgcctccatt aagtggtttg atttatttta ccagaaaaac
      661 aatagttatg tataaaaaac gaacaaaaca caagtaatgt tgtaaccgct atatttatat
      721 gataagatgt ctcaaataat aaaatagaaa agagacataa aaatgattga cttggcaaag
      781 aaattcttac acgaaaatat atattttgag ttgtatcatc atcgagccat ttatacaaat
      841 gaagatgctt tggtaataaa gaaagaacaa ggatttacag gtacagagac caaaagctta
      901 ttcttaaaag ataaaaaaga taataactac gtatatctga cttttactac gaaacaaacg
      961 gatttcaaag cattagggaa gatcgtcggt aaaaggcttt ctattgtttc atcagaacgc
     1021 atggaagaac aaacgggaca aaagcagggc gctgtttctc cgtttggtta tgaaaaacag
     1081 attccgatta ttattgatga agagctctta tcaaatgaaa aacttgtttt tgcaccaggt
     1141 agaccagatc aaacgatggt catccaaacg gttgacttaa acaaaatcat ccagctactt
     1201 gagatgccga tatttttgat gccggcaaac gaaaaaaaca attaaccaag taaaatagtg
     1261 catacttgga ggtgaagcaa catgataagt tatccattat tagaaagaaa tgcaacgatt
     1321 ggggttactg caccatcctc aggcgtatca gaggaacacc attcgttact cgaatcagct
     1381 ttaaagagaa tggaagaaaa atctttccaa gtagttactg gaaagacgct ttggacgcaa
     1441 gataaggaaa aatcaggtcc agcaagaaaa cgagcagatg aattcaatca aatgatgcaa
     1501 gatgaaaata tccaaatgat ctttccgact tggggaggag aattattgat tgagattctc
     1561 gattttgtcg attttgatgc tctacaacca aaatggatag taggttattc agatattagt
     1621 acgttgctac ttgctgtcac attaaaaaca gggatagcca ctgcacatgg aactaacctt
     1681 attgatttaa gaggagagta cttggatgaa acgacagcaa tgtgggaaag tgtactgaaa
     1741 acaagagaag gagaatcgat tctacagcaa tcttctgaaa agtatcaaaa agtgtggccc
     1801 catgagcatt catcatcttc tgttttccat ctcactgaac caacaaattg gcaatcagtc
     1861 agcggagatg cagtaaggat gaagggacga ttattaggtg gttgtattga tacgatcagg
     1921 catttaatcg gtactcctta tggagatgtc caatctttta aaagaaagta taatgatgga
     1981 aagccgatct tgtggtttct agaaaactgt gagctatcgt cagttgattt aagaagatca
     2041 cttgttcaga tgaaactggc aggctggttt gatggcagct cggggatcat gttcggtaga
     2101 agtgcggcag aggatttgtt agattataaa atgaaggatg tatacgaaga tctgtcaaag
     2161 gaattaaaac taccgatctt gtatgatatt gattgtggtc atgtaccgcc acaactgaca
     2221 ttgattaatg gtgcctatgc agaagtagcg gtagaagata gaaaagcgac tgttttgcaa
     2281 acgtttattt aaaaaatttt ttcctgcctt ggagtctatc aaagaccaat aaatctaaat
     2341 agaaaagaaa gtgaaagaag ctgacaaaa
//
LOCUS       DBKOIJ010000246         2351 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000246 DBKOIJ010000000
VERSION     DBKOIJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.254"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            51..2204
                     /locus_tag="ACV5NJ_002793"
     CDS             51..2204
                     /locus_tag="ACV5NJ_002793"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HHY6486812.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          807 a          416 c          527 g          601 t
ORIGIN      
        1 agtttttggg tacaatgaag catgaatgta aataggcgga aggataggaa atgatgaaag
       61 attttatgaa aaaaatatta aatacttctt ggttagaaaa aataagaaaa ggtgcacaag
      121 aacctaagaa caaaacgttt cgccggtccc atgttccttt tcgcttaaat tttctttttt
      181 tcattatatt tacgttattt gttgcgttga ttgctagatt gggttatttg cagatcgtga
      241 acggcgaaga gatggaggcg cgagtgaagt ctacttctac gattacggtc caagaaagta
      301 ctcctcgcgg aatgatctac gactccacag gaaaagcttt agttacgaat aaagcgaatc
      361 aggccatcac atatacgaga ggaacacaag ttacggctaa agatttgctt gaaatcgcaa
      421 cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa tctgacagat agagataaaa
      481 aagatttttg gctggctgat cccaaacatt tgaaagaagc cacaaaaaga ctttcggcaa
      541 aagaaaagca attagggaac agcgaacaat atagtgcgat ggtcaataaa gtcacagctg
      601 atgagattaa tttaaacgag gagcaattaa aaattgctac catctttaaa cggatgaata
      661 gtgcatatga attaaacact gtctatatca aaaactctgg tgttactgat caagagttag
      721 ctgttgtggc agaacgcgct tctgaacttc caggagtttc gactggcaca gactggacac
      781 gagaatataa tgcagcaagt tcactgaaaa gtattcttgg cagtgtgacg actgaaaagc
      841 aaggacttcc agcagatgaa gcagagaaat acctagcaaa aggttattct cggaatgacc
      901 gtgtaggaca aagttatttg gaaaaacaat atgaagatgt cctgcaagga accaaaaccc
      961 aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa tcaaaaagaa atcttctctg
     1021 gggaaaaagg ttctaattta atgttatcta tgaatgcaga attccaatca aaagtagaag
     1081 aaattttgaa aaggaattat caaacattga tcaacaacgg caaagcacag tattcgcctg
     1141 gtgcttatgc agtggctatg aatcctcaga caggagaagt gctagcaatg actggtttca
     1201 gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt aggcacgatt actagcgcct
     1261 ttgatccagg gtctgtagtc aaggctggta ctttgacagc aggctgggca agtaacgcga
     1321 tcagcggaaa tcaagttttg attgatgagc cgattcgtct gcaaggtgct tcagaaaaaa
     1381 gctctgtctt taatcgaagc ggacaagttg ctcttgatgc agtcaaagca tcggaattgt
     1441 cttctaatac gtatatgatc aaagtggcgt taaagatgtt aggactagac tatacacctg
     1501 gaatgggact acctagttta gatgaagaag ctaaagctta tcagcaattg agagactctt
     1561 tcaaagagtt tggtcttggt acaacgactg ggatcgattt gccgaatgaa tctcctggga
     1621 tctcacgttc tgttgattat atgaagaagt tcaatgcgga taatggcaaa gaatggtatg
     1681 ctccaggtaa ctttaccgat ttggcatttg gacagtttga cacatataca ccaatccaat
     1741 tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg agtacagccg cacctagtca
     1801 aagcgatcta tggcaatgac gaaaatggaa acttgggtga agtgaagaaa gaaattggca
     1861 caacagtaga aaatacggtc aatatttctg cagaaaatat ggcgatcctc cgtgaaggat
     1921 tccaccaagt tgtccatggt accgatcctt acacaacagc caaaccatta gccagtgcga
     1981 aaatggacct ttccgcaaaa acagggactg cagaaacggt tgcggaaggt catccagata
     2041 ttgcgacagt caatagtaat atcgtggcat acggaccaac agacaatccg gaaattgcca
     2101 tcagtgttgt cttgccgaac ttattagacg aacaagacca cataaacttg acgattgcta
     2161 aagaaataat ggacgcgtat tatgatatgt tcatggcaaa ataaaaaaga ggtcgtgaaa
     2221 aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa atagcttctc acatttttgc
     2281 ttaaatttga cttcataccg agaaactggc ttttgaacac cgtttactcg gcaaaaaagg
     2341 ttgtgacaaa a
//
LOCUS       DBKOIJ010000247         2326 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000247 DBKOIJ010000000
VERSION     DBKOIJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.255"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            45..572
                     /locus_tag="ACV5NJ_002794"
     CDS             45..572
                     /locus_tag="ACV5NJ_002794"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HHY6486813.1"
                     /translation="MKTIPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIED
                     KEMSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKA
                     DTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNT
                     FVELKKKRRRTQKFL"
     gene            700..1383
                     /locus_tag="ACV5NJ_002795"
     CDS             700..1383
                     /locus_tag="ACV5NJ_002795"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HHY6486814.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1692..1765
                     /locus_tag="ACV5NJ_002796"
     tRNA            1692..1765
                     /locus_tag="ACV5NJ_002796"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1726..1728,aa:Arg,seq:tct)
     gene            1924..2283
                     /locus_tag="ACV5NJ_002797"
     CDS             1924..2283
                     /locus_tag="ACV5NJ_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HHY6486815.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          784 a          354 c          467 g          721 t
ORIGIN      
        1 ttatcgttat tatatgatta agaaggattg aataaagaaa gaagttgaaa acgatacctg
       61 attacaaaaa aataactttt ataagttcta taacgttgat tatattttta attcttattc
      121 ttctcgtcgt cttggttccc cgaatttttg gtttgactcc ttatattatt gaggacaaag
      181 aaatgagtcc atattatgca caaggaaatc tgatatatgt acgaccgaaa aatacaaatg
      241 agattttagt cggagatatt attacatatt atgaaaattc aggaaaaaaa atctcgacgc
      301 gtcgcgtaat agccgttgaa aacaaccacg aagattttta tacaaaagct gatacaaaga
      361 cttatattga gcctggagta gtaaaaaaga gaaatattat cggtagtccc atctttcaga
      421 ttccatatat tggactagtc ctttcaaaaa cggcatttgc atggattcag gggttattct
      481 ttacgattgc tttttgccta acaggaatca cagcatggaa tacctttgta gagttgaaaa
      541 agaagcggag acgaacacag aaatttttgt aagaatgtta aaaacataaa tgtattccag
      601 agatccaacg actcttctga acatgaaaca cgagaaaaca ctcatgcagc ttgtagattt
      661 agtcagttgt tcgtataatg tgtgaagagg aggagtagta tgcgcaatga aatgatgcat
      721 cgtcctgtag tcaaagaaga actattggat tttatgcgta cccagcaaaa aaaattaccg
      781 ggtgagctag gcaaactgga agaagaagct catgtagcag aagtaccagt tattccccat
      841 gaaacagttg tatttttgaa atttttactt ggacagcttc agcctgaacg aatcttggag
      901 atcggagcag ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg agaaaacgct
      961 catgttacga caattgatcg tttcgatgtc atgatcgaga aagctaaaaa aaactatgaa
     1021 cgtctaggac tgacagataa agtgactttg cttgaaggtc aagcagcaga tatcttgccg
     1081 gagttgtctg gtccgtatga ctttattttt atggatagtg caaaatcaaa atatattgaa
     1141 tttttacctg aatgcttacg tttgttaaga aaaggtggcg ttttgatggt agatgacatc
     1201 ttccaaggag gaacgatctt gctcccagat gaagagattc cccgtggcaa acgagctatc
     1261 catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc atcccgattt gacatcgaca
     1321 atcttgccat taggtgatgg tgtgatctta atgacaaagg aatcagaaac aatcgagttg
     1381 tgatacatga ttatggcagc ctgtagataa tcgattgaat cgagggctca attcgaactt
     1441 gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata caggaaatct aaataaacag
     1501 gagtagcatg aagtcaatgt agctcctgtt ttcaattgat aaccttagaa cagctgaagg
     1561 agaatgtctc gttttaaaag attgatttag tagttgggcc aaaataattc attatttgct
     1621 aattaaataa ctatttcgta ttgacagaag caatttaatt atctataatt attgatgttc
     1681 cgctgattct tgtcatagta gctcagctgg atagagcatc cgccttctaa gcggacggtc
     1741 gggggttcga atccctcctg tgacgctaaa aaaccgttag aaactgaggt ttctaacggt
     1801 ttttctttgt tttttgtgcc aatattgttt gttttcaaag gcaaataaaa taaaaaaaca
     1861 tccttatcga gtacaattat aaggagcaaa aaaataaaac tcatcagaaa agaggaagaa
     1921 gatatgacga tactatctag aattttagtg actctagttg caattgaatt tttttatatc
     1981 atgtatatcg aaacggtgag aactgattct gacacgacaa gtagggtttt taaaatgagt
     2041 aaagaagagt taagcagaaa atctgttcaa acattgttta agaaccaagg agtgtacaat
     2101 ggactattag gagtgggact actttatgga gcttatcttt cttccgcatc taaagaaatc
     2161 acaagtatgc tattaatatc tatttttttt gttgcattat atggcagttt gactagcgat
     2221 cgttggattt tactcaaaca aggtgggcta gctctgttgg cattgctttc tattttctta
     2281 taaatataga aaaaataaga atttatctaa caggaaactt tgtgaa
//
LOCUS       DBKOIJ010000248         2312 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000248 DBKOIJ010000000
VERSION     DBKOIJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.256"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            527..721
                     /locus_tag="ACV5NJ_002798"
     CDS             527..721
                     /locus_tag="ACV5NJ_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486816.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            912..1161
                     /locus_tag="ACV5NJ_002799"
                     /pseudo
     CDS             912..1161
                     /locus_tag="ACV5NJ_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1405..2235)
                     /locus_tag="ACV5NJ_002800"
     CDS             complement(1405..2235)
                     /locus_tag="ACV5NJ_002800"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HHY6486817.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT          692 a          421 c          461 g          738 t
ORIGIN      
        1 ttattttata ataaagatat gatgactaat ttagctcaac tattccagcg tcagctgaag
       61 atattgcaat tcccgattcc ttaatcattt atctggatga gacagaaagt gaagttgcaa
      121 cagatgtgca tgtagttgag gataaaaatg atagtttaaa aattaatagt tcagatactt
      181 tactaagagt aagtcctatc ttttcagctg ttatttagat gcaagtagca actaagttag
      241 gtagtactcc tgcaatggat aggtatttgt gtgttcgata tataagtggt cgaagtatca
      301 aggttatgta tactggatgg gagatagaag agttaaaaat tggaggtcac cgccacaaat
      361 ggaatttaga tttagaggta ccttagattt ggtatagtta ttaaacacaa gatggcctgg
      421 tttgtaggga aagtcgaaga gtatttttcc acttctgcgc ccctttaatt tttctagtaa
      481 tgccgtgaat ttgtgattaa ttggaaatat gaagaaagga gagtctatga aaacaacact
      541 agttctattc tataaaaagc atccttattt tacacttctg ataaatatat tgttggctag
      601 tgttatcggg atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta
      661 tacggcttta tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata
      721 aaatagattg ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct
      781 gtcagtgaag cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc
      841 gattgaaata caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc
      901 gtacaaagta gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa
      961 tttataatca atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa
     1021 tataaatttg ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa
     1081 aatgaagcga ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat
     1141 aagctgagtg tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct
     1201 tttaagaggt gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca
     1261 gcgtctttag gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg
     1321 aagggtggtt atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa
     1381 acgggtgttt ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg
     1441 tgttactggt tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata
     1501 ttcgccttcg ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc
     1561 ctctgagaag ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt
     1621 acttgggact aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag
     1681 attttgatga tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc
     1741 atcgatcatt tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt
     1801 agctctaaag tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt
     1861 gcccacgctg tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat
     1921 tccagagata atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac
     1981 aggcgcaccc tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc
     2041 ttctgttgca atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc
     2101 ttggaacagg tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg
     2161 cagatatttc gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt
     2221 ctggtgtcta aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac
     2281 gaaagattaa aaaattcaaa gaaagcgctc gc
//
LOCUS       DBKOIJ010000249         2261 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000249 DBKOIJ010000000
VERSION     DBKOIJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.257"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            278..820
                     /locus_tag="ACV5NJ_002801"
     CDS             278..820
                     /locus_tag="ACV5NJ_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486818.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACV5NJ_002802"
     CDS             976..1425
                     /locus_tag="ACV5NJ_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HHY6486819.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2261)
                     /locus_tag="ACV5NJ_002803"
     CDS             complement(1455..>2261)
                     /locus_tag="ACV5NJ_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HHY6486820.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          784 a          372 c          429 g          676 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgtttcatg
     2101 attcgaccga ttcggcgccg actgagttga agcccacgct ttgctaaaca cttcttcaat
     2161 tttcgggtac cgtaagcctt tcggtttcga ataaactctt cctgaacgat ctcttctaag
     2221 tcggactcat tttcgatcgg tttcgcttga taatagtagg t
//
LOCUS       DBKOIJ010000250         2238 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000250 DBKOIJ010000000
VERSION     DBKOIJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.258"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(171..2204)
                     /gene="brxL"
                     /locus_tag="ACV5NJ_002804"
     CDS             complement(171..2204)
                     /gene="brxL"
                     /locus_tag="ACV5NJ_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HHY6486821.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT          713 a          495 c          303 g          727 t
ORIGIN      
        1 ggcttatagt ataatattta cgccacagcc cccaatgatt ctttttggaa tcatgatcaa
       61 ggacggacat acacaaaaat attttgccca ttcgatccga atagattaac gactaatcta
      121 gtaagtaata tagattggta cgtagtcaaa ccagcaaatg gaaaagttgt ctattccacc
      181 ccaagggctt taaagacagc atcttccggc gtttgataaa aaatcaaatt aaaggtaccc
      241 attaattctg ggggtacagt tccaatatca gcagcgctta tcataggtac aagtactttt
      301 ttcgctccgg catcttttgc tacttgtaaa cagttagcta aattatctac cttaatcata
      361 gaaccaccaa ttgtaatatc ccctaaaata actaaacttg gtaatacagg tttatttaag
      421 gaaactgagc ataaagctat tatagttgat aacgataatg tttttgtcat accaatgcca
      481 ttcatatctt gaacatgaac gacaaaatct tttgtcgtca aactaataga ctgactaaca
      541 cttccataat tagctcttaa atatcggtat gccgaatcca tcgcttctct tgctccagca
      601 tctgaattga tcccagtacg ttcaaacttt ccatttcccg aagtcatttg cgtttccaat
      661 ttataggtac ctaattgccc agttttgcca taagataccg tgtacaaatg ccctggcttc
      721 cctaaccctt ctggaatcaa agttcctcca ccttgttcag gaacagaaac atactcttct
      781 cgctcttctt ctaaatcaat gtatgaaaaa ttgacatcat aaaattccat tccaccaatt
      841 ttctttaatt gttcttttac acgacgacgc atttcaagag caaatcttag tatttcttcg
      901 acatcacttt tttcaaatct accatcagga tacaacagtt tcatcatccc agatacaatc
      961 cgacgaactg caatcgtatc ccgctgattt aattgagaac caagtttgaa atatttatca
     1021 aaactatcgc caaaactaac ttttcgaagc tcacgcatga cttcagaaaa ataatctgta
     1081 atgaagccat agtcatttgt aaaaaaatct ggtcgatatt taggaatttc ccaacctggt
     1141 aaataacaat gcatccgatc caaaaaggct gaatccgtcc ccataacctc cggaaatggc
     1201 tcaaataaat tcgaagtttt caatattaca tcaacgcttt gattaatatt tcctacaaaa
     1261 gccattgatg ctgaagcatt cttttcttct ttgcctctag caaaagatcc tgaagccatg
     1321 taatctttca tgatttgaac gccatcatta tccttaaatt taatcccagc aacttcgtca
     1381 aaagcaacac agtcccacat acctacaagt cccattgtac gactagccat attatagaaa
     1441 aggtttgcta ctgttgtttg tccacctgaa acaaggattg agtttggtga aatttcttta
     1501 tatacatgcg atttacctgt accacgtggt cctagttcac agagattaaa attattttca
     1561 attaatggaa taagtcttgc taaatgaagc cattttaccc ttttattaaa ttgtgtaggt
     1621 tccataccaa tggaccgtag aagtaagtca atccattcat ttgtagtaaa ctccttgcga
     1681 gccatcttgt aagcctcaat gtctaaacca ggcatttgga ttggatcaac cttttttgca
     1741 ataaaagggc tattccaacg atcattctca tcataccaat attccatcga aacaatacac
     1801 cagatccccc cacctaataa tcgatcaaat tttttcggat actcttcact aatttgaaca
     1861 ttttttaatc ccaggttaga gaattctgct tcataacaat ctttcttttc atttaaatgt
     1921 acagttactt tatcaataac tgtataacca cccaattcac gaatttttgc ttttactttt
     1981 tctgcttcat caggacgcac atagtttcga gcaagaatat ttttaacatt ttccacacct
     2041 ttttcaacca atgtttcatc caatgacgaa gcatacatac caagtaaata ttccaataca
     2101 taaacaggta cattcgcacc ttctttaatt ttctttgtta aatcttttct gacaatcttt
     2161 ccgggaaaga attcagataa ctttaaaatt tcctgttgtt ccactagaat tcctccatgt
     2221 gttcagtcat tataatag
//
LOCUS       DBKOIJ010000251         2227 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000251 DBKOIJ010000000
VERSION     DBKOIJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.259"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..280)
                     /locus_tag="ACV5NJ_002805"
     CDS             complement(<1..280)
                     /locus_tag="ACV5NJ_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HHY6486822.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="ACV5NJ_002806"
                     /pseudo
     CDS             742..2124
                     /locus_tag="ACV5NJ_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          654 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaa
//
LOCUS       DBKOIJ010000252         2212 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000252 DBKOIJ010000000
VERSION     DBKOIJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.260"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(696..812)
                     /locus_tag="ACV5NJ_002807"
     CDS             complement(696..812)
                     /locus_tag="ACV5NJ_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HHY6486823.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(1288..2166)
                     /locus_tag="ACV5NJ_002808"
     CDS             complement(1288..2166)
                     /locus_tag="ACV5NJ_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HHY6486824.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          696 a          388 c          359 g          769 t
ORIGIN      
        1 aaaatttcat gaaaaatact ttattactgt atctaaaatt ccaatacaca acttatcgac
       61 tattatctga aaaccaaaac ttattattcc ttgatgttat tctagcaata gtaactgctt
      121 ttttattttt ttgcatattt tttcggcgtt actttttaaa gttaactttt tcgacaaata
      181 gaaagagata cgaatcactg atttttttga agaaaagaaa tggagtgagc tgaacgttag
      241 agatattggg agctttcgta actgcttatt aaacgataat ggttatttgc aaccgtattg
      301 ctcgcttcta catgaaatat ttcatgaaat atttcgcaaa acctttgatt atgtagttac
      361 gtttccattc ttgactaaaa atgtttcaaa aacgaacaaa ggtgttaaaa ggtaaagtga
      421 tcaatactac tggtcaaaat aagaggttga aagggaattg aaaaataact acagataata
      481 cttttataaa cgtacgtgct ttgtggttct gtagttaact atataactgg aattagggaa
      541 tggtatcaat ttgaaaagaa agattctacc aatttatcat atgttaaaaa tggaaaatca
      601 acagcagttc tttcggaaac cttacacaaa aaccgattat tgaaaatagc cgaaaaacgt
      661 ttttcggcta tttttttaat taagtttcct tcttcttacc ttttcccact attccgtttc
      721 acatctttga tgatcttcag cgcacgtttt cgtgttttga tatttgggct tttcaagcgg
      781 cggtatgcac ttgagatatc tgttttttcc atatggacga cccactttct tctttgattg
      841 tagtcatgct aaaaaacaca aatcgaaaca attacgattt gtgttttaaa tggtatcaaa
      901 agttaaaatg aaagtcaact tgttaaaaca gtcgatcaga ctagctgatt gactcctatc
      961 tctggaaata aattttcatt attacgtttt agctacctaa aaaaggccct cttgtccgtt
     1021 aactttaata tctattgtta tagctagttt acctattgaa cgtactgtgt gttcgataga
     1081 acactaaaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa gaaattcacg
     1141 aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt
     1201 ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtctcacgct
     1261 cttttaaact atttcgaagt actaaactta tgaagtaaga atacctaagc cagagccaat
     1321 gtgctcgcct gcaagcaatt gctcttttgc ttgtcgctgt aaactcgtac tttctggata
     1381 atcatgtatt tgggcatcta ctcgtccaaa gatccaaggg actccactat cgtcctgttc
     1441 atcaactgat aataatcgat aatcggctgc aaaaagacag atttttgact cttttttcaa
     1501 ccatttagta agattgggtg atagtaacca agtatctgtg tacatcactt ggtaatggta
     1561 atcaggaaaa taaagttcaa aaaaacgaat ggcactttcg taactatctc ttatcttatt
     1621 ctgagataaa tctgcatcgg aaggaatatg aagagaaagg cactgctttc cttctaatct
     1681 tagatccgtg taccctttcg gaaaatatgt catttcgtac tccaattcac caatccgaaa
     1741 gatcaagcca ctagtgtaat gccaaatcca aaaaccacgg tcaaagcgat agcttcctgt
     1801 tacttggaaa tgttcatgaa taaatctagt aaaagcattc attgttgcca aatagatagt
     1861 tcttggaatc ttcaatggtt gataaatttt tttccaactt tgagaagctg catatagata
     1921 aatagctaat gctgcaaaag gttcctcttc atagactttg tctattttct ttgcagtcgc
     1981 ctttgcttta ttaggatcca ttagttcatg actatattgc tcaaacacct catcaaggat
     2041 gttttttgct tgtacaatca tttgctctat aacgactttg ggaagagtta aagtttctgc
     2101 cagaatttgg atttcattgc ttattttctt catcaaattt ccccttttca tatctttgaa
     2161 cctcattgtt taaatgactg attatccgga caccatatgg tataaacata ct
//
LOCUS       DBKOIJ010000253         2180 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000253 DBKOIJ010000000
VERSION     DBKOIJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.261"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            15..2180
                     /gene="acm"
                     /locus_tag="ACV5NJ_002809"
     CDS             15..2180
                     /gene="acm"
                     /locus_tag="ACV5NJ_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HHY6486825.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          859 a          348 c          423 g          550 t
ORIGIN      
        1 gggagataga aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta
       61 ctaatataac atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca
      121 gtaatgtcac ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag
      181 taaaattctc gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca
      241 attggccaag tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga
      301 ttgaaaataa aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta
      361 atgaaaatat taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca
      421 gaaacgtaac cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata
      481 aaacagctac tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt
      541 ataaaacagg agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta
      601 acaataacgg cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac
      661 aaagattaga tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt
      721 atagaggaga agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt
      781 tttcagtaac tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga
      841 aaataatggt gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc
      901 attcggaagc ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc
      961 atacagttaa aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga
     1021 agattttcaa atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa
     1081 gacgagcaga tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac
     1141 agggagaagc tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag
     1201 cacccaattg gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg
     1261 aaaatgatac agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca
     1321 cagcaactaa aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat
     1381 tcagagcaag tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca
     1441 atggaataac ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat
     1501 atacgtttaa agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc
     1561 aaaaaattga aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag
     1621 gacaaaaaac atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag
     1681 taaatctatt agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt
     1741 ggcgctatcg tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg
     1801 taacggaaga caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca
     1861 gttatacacc ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa
     1921 attttccaga gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa
     1981 tatttgctca tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg
     2041 tacctaaaca gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat
     2101 ctagttttgc tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac
     2161 aaaaatataa aaaagtttag
//
LOCUS       DBKOIJ010000254         2171 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000254 DBKOIJ010000000
VERSION     DBKOIJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.262"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..914)
                     /locus_tag="ACV5NJ_002810"
     CDS             complement(<1..914)
                     /locus_tag="ACV5NJ_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HHY6486826.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACV5NJ_002811"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACV5NJ_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HHY6486827.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          736 a          383 c          416 g          636 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat c
//
LOCUS       DBKOIJ010000255         2134 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000255 DBKOIJ010000000
VERSION     DBKOIJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.263"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(399..1565)
                     /locus_tag="ACV5NJ_002812"
     CDS             complement(399..1565)
                     /locus_tag="ACV5NJ_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HHY6486828.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(1620..>2134)
                     /locus_tag="ACV5NJ_002813"
     CDS             complement(1620..>2134)
                     /locus_tag="ACV5NJ_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HHY6486829.1"
                     /translation="TGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDET
                     NSIEQPNLDRIKTARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSD
                     FLFLKSILEDSFEYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKRE
                     ITARLISKLQ"
BASE COUNT          652 a          337 c          343 g          802 t
ORIGIN      
        1 attaagcaca aaaaaataag atgctcgctt gaacatcttt gtttataaag tctttttaaa
       61 atttttattg actgaataga atggatttga gaatgaatga gcctaaaaat ttaatctaaa
      121 atacgaaata tactgccttt aaattagaac acattttaga acacaaacat ttcaaattta
      181 atcaaatata ataaaatata tttacgggaa aacctttata aatcaaggtt tttcaaactt
      241 attcaatgat acgaagaaaa aaccaggtct taagaaagca cgtaaagctt cacagttttc
      301 aaaacgttaa tcgttgttat accaacgatt tcagacactt tctcattgcg agaaggtgtc
      361 tttttttcgt tctggtaatg agaaaggtaa tgacaatatt ataaaccagt gtaagcaatg
      421 aatttttcta acgcatttcg ttgagctttt tcagtaacat gagaataagt gttaagggta
      481 gtttcagcat ttttatgacc caatcgatct tgtgtttctt tgatacttgc tccaccttct
      541 agtagaagag tagcgtgagt gtgccggaat ccatgaatag taacgtgctt aaatttatat
      601 ttcttctcta aacgtttcaa tatattattt gaatagtccg catgcaaagg ttgattaatg
      661 tcaccgtctg catttacata ggtaaagata agttgatttt ttgtatttgt atttatgcca
      721 aactgcaaaa gctctcgagc ttgttctttt ttccagattt ttaacatttc aatagtttgt
      781 gcatcaattg gtaatattcg ttcggaagat atattttttg tatttttttg aatcctgaca
      841 gtttttcttc ttgcattctt ctcttcattt cgaatagttg catagctctt tctaacatac
      901 attagctgtt cttcaaaata aatgtcagac caatgaagag cagctagctc tcctttacgt
      961 ataccagtag taatcaaaag ctggaagatt aaaatatgtt ttaaaggctc ttctttattg
     1021 agaatatccc aaaaagcttt tagttcttct aatgaaaagt aattttcatt ttctttcttt
     1081 tcccgctttg gtagatttgt atttgccata ggatttcggt gtaatagctc tgcaaaaatg
     1141 gcatactcaa atatttgaga gctgtaagac ttaagctgtt tgatattctt gaattttaaa
     1201 gcccattcat tgactgcatt ctgacagtga acaggagtaa tcttagaaat ggacttattc
     1261 ccgaattttg gtagtatatg caagcggaaa aggtcttttg tacgttgata tgtcgtttct
     1321 tgtacacgat tcctataaga aggcaaccat tcttcataca ctgttataaa caagtcatta
     1381 gaaggcttat gatagccggt atcttcaatc tcgacctttt ttcttgcttc gtgtaacttg
     1441 gcttcttttt cagttttaaa cccttttttt acatagtatt cttgctttcc attttcatct
     1501 ttacgaacat acaagcggac actatatcgt gcaccgcttt ttgttgtgta cttttgtatt
     1561 gacataacta atctctccaa tctgataact tggcgagttg gctgattgaa taattagttt
     1621 tattgaagtt ttgatattaa tcgagctgtt atctctcttt ttagattgtt aacctgtact
     1681 tgggttaagt tgccaaataa ctcagattct aactcttttc tttcatcgat atttaagttt
     1741 aaagctttat taataaactt cttttgtttg gcaaatagaa tcgtgtattc aaaggagtcc
     1801 tctagaatac tttttaaaaa aagaaagtcg gagtcatctt cttgaagttt gacttcaagt
     1861 aaagaagtca tggaaaagta tagattagat aattcagtaa aataatctga gtcaagactg
     1921 gcaatattct tgactatttt gtcctttttt tctaaacgag cagtttttat tctatcaaga
     1981 ttaggttgtt ctatggaatt tgtttcgtct aatcccatca aataacttac aggaacatgg
     2041 aaataatcag caaggatatt ccatgtttca gtatttctag gatttcgctc accacggtaa
     2101 tagttcgtaa gagttgtata gttaatacca gttt
//
LOCUS       DBKOIJ010000256         2097 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000256 DBKOIJ010000000
VERSION     DBKOIJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.264"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..496
                     /locus_tag="ACV5NJ_002814"
     CDS             <1..496
                     /locus_tag="ACV5NJ_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486830.1"
                     /translation="RLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDSGLA
                     TELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIK
                     RKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTY
                     DILA"
     gene            complement(557..922)
                     /locus_tag="ACV5NJ_002815"
     CDS             complement(557..922)
                     /locus_tag="ACV5NJ_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HHY6486831.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(998..1243)
                     /locus_tag="ACV5NJ_002816"
     CDS             complement(998..1243)
                     /locus_tag="ACV5NJ_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486832.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1313..1567)
                     /locus_tag="ACV5NJ_002817"
     CDS             complement(1313..1567)
                     /locus_tag="ACV5NJ_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486833.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1731..2054)
                     /locus_tag="ACV5NJ_002818"
     CDS             complement(1731..2054)
                     /locus_tag="ACV5NJ_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486834.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          782 a          361 c          370 g          584 t
ORIGIN      
        1 tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg gggggaacct
       61 tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag gttgttctga
      121 ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag aggagttaaa
      181 atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg gaatattagg
      241 cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa
      301 aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga
      361 tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag ttggcgatga
      421 accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc ctaaaactta
      481 cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga ggagatagaa
      541 ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt gctaaacatt
      601 ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct acagtaggta
      661 aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac tctggtgctg
      721 ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg aataatctat
      781 aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag ccatttcctc
      841 ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc tttgaaatca
      901 atttttcatg ccataatcgc atagaaatgt ctcctttata tatagattgt taaccttaga
      961 cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg cttgctgttt
     1021 ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt
     1081 caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat aaaggaatac
     1141 ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag aaattcagac
     1201 gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca cctttcatta
     1261 tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt actcaatgtt
     1321 ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc agtcctgata
     1381 attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact ttccacataa
     1441 agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc caattcataa
     1501 gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact ctcaccaacg
     1561 taatcatgta acggactcct caacgattca tataataaaa gataacatgt tattgcatac
     1621 atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa tgaaaaagca
     1681 gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa
     1741 aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac gatcgcattc
     1801 actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac aaatactttt
     1861 gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag aagcaaaagg
     1921 gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg ataatttaat
     1981 tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata
     2041 atcactccta acattgagaa taaataaaag aataacattt tttctaaaat aagcaaa
//
LOCUS       DBKOIJ010000257         2096 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000257 DBKOIJ010000000
VERSION     DBKOIJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.265"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..430
                     /locus_tag="ACV5NJ_002819"
     CDS             <1..430
                     /locus_tag="ACV5NJ_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HHY6486835.1"
                     /translation="IDLPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFL
                     IKDYAISKNSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTN
                     KDVHDMDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(576..1052)
                     /locus_tag="ACV5NJ_002820"
     CDS             complement(576..1052)
                     /locus_tag="ACV5NJ_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HHY6486836.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(1012..1368)
                     /locus_tag="ACV5NJ_002821"
     CDS             complement(1012..1368)
                     /locus_tag="ACV5NJ_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486837.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1346..1531)
                     /locus_tag="ACV5NJ_002822"
     CDS             complement(1346..1531)
                     /locus_tag="ACV5NJ_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486838.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1532..1804)
                     /locus_tag="ACV5NJ_002823"
     CDS             complement(1532..1804)
                     /locus_tag="ACV5NJ_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HHY6486839.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1801..2001)
                     /locus_tag="ACV5NJ_002824"
     CDS             complement(1801..2001)
                     /locus_tag="ACV5NJ_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486840.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT          629 a          395 c          297 g          775 t
ORIGIN      
        1 aatagattta ccagaaaata ttgaaaaagt ttctcctaca tttgttgata tttatactca
       61 agccacacaa gctaaatctg agcaattgga acaaattgct ggagttggtt atagaaaagc
      121 tctagaattt ctaattaaag attatgcaat atctaaaaat tcagaggatg aagataaaat
      181 aaaaaaaatg ttattaggta aaacaattga aaattatttg aatgattttc ctaaactcca
      241 aagattagcc aaagctgcta cttggattgg taatgatgaa acgcactatg ttcgtagaca
      301 tactaataaa gatgttcatg atatggatgc atttattaaa tcagcagcgc aatttatcgc
      361 ggctgattat gatgctgatt tagctgatca atttataaat ggaaataact ttatgccaac
      421 tactaattaa aagaataaaa gagccacaga taccaattaa taaaacggtt gggatatctt
      481 ggctctttca tatttttttg acactaatag aatatcatgc tgctaacaat gtttacatag
      541 ctacaatgac cgaacattta gtgaacagtg taattttaga ataaagagag ttgttctttg
      601 tacttctcgc tattgtaaaa atcaatttcg ttttcttttt tcaatcggtc agcttgtctg
      661 tgttcatact catctaaaaa gttcaatgtc tttctaattt ctgcatgtcg ctgtctgaca
      721 tacgatgagc tatacccaac agattcagca atatcttcta agctcatttg atcaacatat
      781 ttcatcttaa caatttgatt atcgatacca gaaaagctat ctatcagcaa taacatttct
      841 tctttttgct cgatcaacaa ttccaattca ttttctatct tttggatatt ctcttctaaa
      901 gaagaagttc tcgaattctt ttcgatgcgg acgtttgcta aatcaccaca gacccatcga
      961 ttcaattcaa gcttactttt attaagattc cactttaagt aaagaatctg ctcatctagt
     1021 tcttggtaat cttttaacca atgaaatctc acaaacgcca ccccttatgg tagaatagta
     1081 ttgcgctgct atctcgaaag gggtagcttt tttatttttt cttagccgtt ccattctgga
     1141 ttgccttttg ttccaaacgt cgttttttct tcttaatctt tgattttgtt tttcccatac
     1201 aattctcctt tccgaaaaca cattttgttc gatttttaat actaaataaa agttggccag
     1261 aatttttcgt tcgtctttac tcatcttttt ttgaataaga ttactagcat aaaaatagtt
     1321 agtaccacga aaatgaacag gtaaatcaat tacgttcacc ttcttcatgc cagttatcag
     1381 ccactagaat ctttgcgtta gtgccgccac cgttttcttt tttgtatttt tcaaatgctt
     1441 tataagcagc tgtttttgat ttatagattt ttccggaacc gactgggaac atccatgtgc
     1501 cctctgtgat agctaagatg tatactttca tttaattcac ccctttaatc cccaacacta
     1561 catatccgga cttctgcatg taatcagtaa tataagtgat ctctgctttg tatgagtcac
     1621 cagtgtaact aaatgttggt ctcgttagtc caggtaattc atgtttaatg tattttttat
     1681 attcacgtag tattaactga tcaccaactt tgaagtcacg atcattcttg cggatctcga
     1741 attgtttgcg tccgctcgtc actgcttcaa aatattctgg tagtattttt agttcgtgga
     1801 tcattctgtt tcctccttca aaattcgctc atcatcaaaa agtgaatctc cacaaaatcc
     1861 ttctcccaaa tatttatcgt tcatgtataa gtcaaccaaa tatttgtgat ctgcttctcg
     1921 atattcatta atattaacga tctttatttt gtaaacatta ccttcgtcgc tcactatctt
     1981 gaattgttct ctgactttca ccaccgttcc tcctcttttt gaaaaaattt tagtgttttt
     2041 gtaagacccc ctaaatcagc ttttttattt gatataatac ttgtatcgat taatca
//
LOCUS       DBKOIJ010000258         2075 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000258 DBKOIJ010000000
VERSION     DBKOIJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.266"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(112..900)
                     /locus_tag="ACV5NJ_002825"
     CDS             complement(112..900)
                     /locus_tag="ACV5NJ_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486841.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(1006..1806)
                     /locus_tag="ACV5NJ_002826"
     CDS             complement(1006..1806)
                     /locus_tag="ACV5NJ_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486842.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
BASE COUNT          609 a          409 c          235 g          822 t
ORIGIN      
        1 aaaaaggatg gcaaaaaatt atcaagttaa aaaaataaat tttgaaccaa attattttta
       61 aaaaaatagc tagttttttt atcaaaaaaa atagagcttt tcagctctat tttattcaat
      121 atcattttga tcaattaata atcgtacact ccaattaacc gagttttgat cactcaaata
      181 tttataatct ttgtcatcaa aaaccagttg ccaaactctg gtacgaacag tttcttcgtc
      241 cacttcctca tctgaagagt attgcgataa tgcttcttca actatatact ttacaacttc
      301 ctcaaaatca gtaggaactt tatcaataac cacttcatag tcttcttgtt ttacgatatt
      361 gggatcaata tgttcatcca aacatacctc agtgagtttt tctttcacct ctgtatctct
      421 ttctgttttt aggtactctg catagtcttc cttagaccat tgtttttcaa gatcatccgc
      481 tatactttta ataaaggatt ctttatttaa ccaattatct gcggtatcta taccttcaaa
      541 ctcaaaagct gttccttcta tacgtgattt gacgaaagca tcaattttct tgtattgatt
      601 attcatttcc catgaaacac actcatcttc ttgcgtaata tcataaattt tttcggaaaa
      661 actaactaat tcattccacg atttatcctc ggcaaattga cttagtttcg aagcgataaa
      721 aggaaatttt gcttttaagt catccatatt cttgatctca aattcgatat tttcttctcc
      781 tacagctgaa atccatgctg aaagattgat tgcaatatcg ctaaagtctt ctacattttc
      841 gacatttcct gtattatctt ttattactgc ttctaaagcc atatcggttg ttgcactcat
      901 ggtttgatct cctctcataa tagagcaaaa agccggttta accgactttt tgcatggtac
      961 gttttgtttg gtagttttct gcttgtgttt atgttgaaat aatccctata gctcttcttt
     1021 taatagttct tcaactttat ctcttaactc tccctgacca ctctctaata gatttaaata
     1081 gctcatcaga atatccgttt catacgtttt taattcagct ctatactcac tttctgaata
     1141 tgatcgtaaa tacccatcat tgctaaatgc aactagatca tcttgaaagt tccattctcc
     1201 taaatttgtt ttactcactg cttcccatgg actattacta aacatttcat taaagaaatc
     1261 ttcgtcattt tgatatacct tcaattcagg aaaatagttg ctgtcttcat tgatttcttg
     1321 caccattttt ccaagttctt cttcagataa tttaccttcc aataatttta ctttttctct
     1381 gtcatttgca taagccattt gaacctctcc tttttctttt tcaatttctt caggtgtttg
     1441 ttttggcgta actttcaaat ctattccatt gcctattaga cctattagat aaccatagaa
     1501 cgttttaaag tcacttgtag caagagattt ggcatcaggg tatgtatccc ctccaatttt
     1561 atctaaaaca aaatcagcaa tcgttaccca aggagaatat atcgtacaca ctacatccca
     1621 tgtagcatct tcaatattcg tccatgtcgg aaagtcattt tcattcaaat agtctttttt
     1681 cattgtttcg atcagagttt ttccactttc gttttggctt agatattcaa caaattctgc
     1741 actatcaaac ctgctactaa atagtttagc taatgagtct aaagctgttt ttgatattcc
     1801 caccattact acacctccta ttaatatttt acgatgactt gtttttcatt cacaaataag
     1861 attttctaca tcttatctat ttctttatag cttttccata cattatttct aaatacaacc
     1921 aataacccta ttaaattaag cttttgagtt tatcttttct aataatattc tgataaatat
     1981 tctgataaaa aaatttgttg tatttttttc ttgacttttt ctttagaatt aaaatcagtt
     2041 ttgattttat ttaaaagtga atctagctct attca
//
LOCUS       DBKOIJ010000259         2019 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000259 DBKOIJ010000000
VERSION     DBKOIJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.267"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            100..570
                     /locus_tag="ACV5NJ_002827"
     CDS             100..570
                     /locus_tag="ACV5NJ_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HHY6486843.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACV5NJ_002828"
     CDS             792..1895
                     /locus_tag="ACV5NJ_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HHY6486844.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DBKOIJ010000260         2003 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000260 DBKOIJ010000000
VERSION     DBKOIJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.268"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..378
                     /locus_tag="ACV5NJ_002829"
     CDS             <1..378
                     /locus_tag="ACV5NJ_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HHY6486845.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACV5NJ_002830"
     CDS             426..1826
                     /locus_tag="ACV5NJ_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HHY6486846.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DBKOIJ010000261         1979 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000261 DBKOIJ010000000
VERSION     DBKOIJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.269"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(346..528)
                     /locus_tag="ACV5NJ_002831"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACV5NJ_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACV5NJ_002832"
     CDS             complement(728..1744)
                     /locus_tag="ACV5NJ_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HHY6486847.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DBKOIJ010000262         1954 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000262 DBKOIJ010000000
VERSION     DBKOIJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.270"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="ACV5NJ_002833"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="ACV5NJ_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HHY6486848.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="ACV5NJ_002834"
     CDS             850..1038
                     /locus_tag="ACV5NJ_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HHY6486849.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="ACV5NJ_002835"
     CDS             1051..1608
                     /locus_tag="ACV5NJ_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HHY6486850.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="ACV5NJ_002836"
     CDS             1640..1882
                     /locus_tag="ACV5NJ_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HHY6486851.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          713 a          305 c          404 g          532 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 atttgagaac cgtagcgaca ctcgctgcgg ttct
//
LOCUS       DBKOIJ010000263         1944 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000263 DBKOIJ010000000
VERSION     DBKOIJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.271"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            23..96
                     /locus_tag="ACV5NJ_002837"
     tRNA            23..96
                     /locus_tag="ACV5NJ_002837"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57..59,aa:Ile,seq:cat)
     gene            complement(481..978)
                     /locus_tag="ACV5NJ_002838"
     CDS             complement(481..978)
                     /locus_tag="ACV5NJ_002838"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HHY6486852.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1018..1827)
                     /locus_tag="ACV5NJ_002839"
     CDS             complement(1018..1827)
                     /locus_tag="ACV5NJ_002839"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HHY6486853.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          561 a          362 c          341 g          680 t
ORIGIN      
        1 aaaaataaat tttatttatg atgtaccttt agctcagttg gttagagcag acggctcata
       61 accgtccggt cgtaggttcg agtcctacaa ggtacataag aggttgtgaa aaagctgccc
      121 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta cttggctgtt
      181 cttattactg cagatgcaag cttttgaata ccgtttaagg aagaggttgt gaaatcagcg
      241 ttttgacctg agtattagct taacaaacgg aaaaatagtt cctcatattt tgccgtttgt
      301 taagctaata ccgagggtca gctgattgaa caccatttaa gaaagaggtc gtgagcctgc
      361 cgtttcactc tgtgtcataa aaaagccaat ctactttgat agaaaaagca cgtaggacta
      421 aaacagttct gcgtgctttt tttattgtgt gttaaatatc gtaaatgaag atagatattt
      481 ttatgattcg acgatagaga cgttgaaatc tttcaacagc tctaaagcgg attctccgtg
      541 ttttctactg gagactaaca ttggatcaac aaaaaagtct gcttcaggaa aagcactctg
      601 caggcctatc agattagaga gtacacacaa gtttgtttca atcccgacta cttcgatctc
      661 tttttgttct tctttgcttt caaataaagt ttcctggatt tcagctaaag tttcaggtgg
      721 aatcgtaaag taatactttt taatcactcg ttcattttct tgtggagaaa gaagagggat
      781 caattgcaaa tcttctcgtt cttcttcttt gtccttcagt tcaactggta tatcccgtgt
      841 gaagagaata tattcattat tttggcgtgc tttagctaat ctttgagcaa tacgttctgc
      901 caattgatct gactctggaa tataaaattc gctgtttgga tcaagaatat gattttgcat
      961 atcaataata actaacattt tctttcctcc tacattcata tcgtcatttt attcttttta
     1021 atagaagttt tttaaaatat aagcaacacc agcttcttca ttcgtcagtg ttacttggtt
     1081 tgcaatttct tttacttgtc gctgtgcatt ccccatcgca atactcaacc cgctgtaatt
     1141 caacatagca atgtcgtttt cttgatcgcc aaaagccatg atgtcactcg attcgattcc
     1201 aaggtaatcc gccagaaaaa caagtgaatg agctttagtg atacctgtag gcgtgatttc
     1261 taagaaatgc ggatcagaga atactgattg gataccatct gcttgcagat ctctttgtag
     1321 cttctgtagt ttttcttcac tcttatctgt taatgctaat ttaagatagc taagagaagg
     1381 atcttccaac atatttaaaa tttgatcaaa agaatggttc tgtaaattga aatgttcata
     1441 ataagggtca tctttgacta gatttgattc atcgaatgtc agctgatcat cttgggtttc
     1501 tcggctagca atgatggata ttttcgcttc ttgagcatag aagaaggctt ttctcagtgt
     1561 atcgctatct atttttgaac gtctgagaac tttcgccatt tttccatcct tcatttcagc
     1621 aatcagtgaa ccattggaag aaatcaaata gccaccgatt cctaattcgg aaaaaatatc
     1681 tttagcagaa atgctgttcc ttccggtagc gacggttacg attccgccat tttcatcaaa
     1741 tttccgaagt gcttggatat tttcttctgg tatctttcca tttcgatcaa gcaaggtacc
     1801 gtctaaatca atcgctgcta gtttcatttt atcttcctac tttcttttta catatctatt
     1861 ttcctgttac cttaagatta aatgagaatt taaaaaaatg aaactaactt gctttgtcat
     1921 tatgaatttt ttcaaaaaac attc
//
LOCUS       DBKOIJ010000264         1878 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000264 DBKOIJ010000000
VERSION     DBKOIJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.272"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(68..1624)
                     /gene="rny"
                     /locus_tag="ACV5NJ_002840"
     CDS             complement(68..1624)
                     /gene="rny"
                     /locus_tag="ACV5NJ_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HHY6486854.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT          473 a          426 c          322 g          657 t
ORIGIN      
        1 ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc ttcttttgta
       61 ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt actttgatgt
      121 gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact aaacgaacag
      181 cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt ccagcttgaa
      241 ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga cttcttaaac
      301 gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct gagagcgcat
      361 ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca tgatgagaag
      421 cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc gcaccgatct
      481 caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg agcaacccgg
      541 cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca gctaattttg
      601 caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat cgcagacgtc
      661 cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac gctgcctctt
      721 ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc atttcttcga
      781 tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc cgtgcgattt
      841 ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca tcgatgatta
      901 aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt ccaatgatac
      961 gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct gaaacttgat
     1021 ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga tctgcttctt
     1081 ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc agctcttctt
     1141 cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca attttttcta
     1201 gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct tcaattaatt
     1261 gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt ttttctaacg
     1321 agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct tgagatttta
     1381 attctaactt actttctctc agctcacttt caacttcaga acggtatttc tgattttctt
     1441 ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt gcactatcga
     1501 tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga
     1561 ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga atgttgaata
     1621 ccataaaatc cacctccata ctatcttttt catttttatc atctatacag cttctatgat
     1681 aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa aacatgcaca
     1741 actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag aaacggcttt
     1801 ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat ataggtaaac
     1861 ctttaaattg agaaactt
//
LOCUS       DBKOIJ010000265         1857 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000265 DBKOIJ010000000
VERSION     DBKOIJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.273"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(34..735)
                     /locus_tag="ACV5NJ_002841"
     CDS             complement(34..735)
                     /locus_tag="ACV5NJ_002841"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HHY6486855.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(751..1191)
                     /locus_tag="ACV5NJ_002842"
     CDS             complement(751..1191)
                     /locus_tag="ACV5NJ_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HHY6486856.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1191..1766)
                     /locus_tag="ACV5NJ_002843"
     CDS             complement(1191..1766)
                     /locus_tag="ACV5NJ_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HHY6486857.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          520 a          400 c          288 g          649 t
ORIGIN      
        1 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
       61 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
      121 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
      181 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
      241 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
      301 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
      361 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
      421 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
      481 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
      541 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
      601 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
      661 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
      721 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
      781 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
      841 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
      901 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
      961 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     1021 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     1081 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     1141 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     1201 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     1261 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     1321 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     1381 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     1441 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     1501 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     1561 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     1621 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     1681 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     1741 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     1801 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtat
//
LOCUS       DBKOIJ010000266         1821 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000266 DBKOIJ010000000
VERSION     DBKOIJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.274"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            18..440
                     /locus_tag="ACV5NJ_002844"
     CDS             18..440
                     /locus_tag="ACV5NJ_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486858.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(606..1799)
                     /locus_tag="ACV5NJ_002845"
     CDS             complement(606..1799)
                     /locus_tag="ACV5NJ_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HHY6486859.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
BASE COUNT          651 a          392 c          310 g          468 t
ORIGIN      
        1 atacaggagg agaaaatatg cagacttttt taacaaaagt ttctcaaaag aaaattaaaa
       61 tgattcattt actgcttgaa acagaacgtt ggtgttcaat agaagaacta caaaacgaat
      121 tgaaggtatc aagtaagtct atcttgaatt atctaacaga tttagaagaa ttgttccaaa
      181 aatatcctga taaggtcatt ttgaagaatg agaataatcg gcgcttttct atggaaaagc
      241 aggagaactt cccaatctac attatttatc ttcatttcta cagaaaatca tataattatc
      301 atttgattga attcatgtat caacatcctg agaaaaattt ggaagattat gcagacgctc
      361 aatatacgag tgtatccaca gtttttcgtt atgctaagtt attagtaaag tactttgaaa
      421 tgacggacgt caaagaatag tcgccgccag atagaaggaa attagcagtt gaaaaagctg
      481 ccctgcatca agtaatacga ccagccaaac ggaaacagtg tctcatgttt ttgatttggc
      541 tggtcttatt accgcagatg caagcttttg ccacacctct ttcttgtcat ttacagactc
      601 aactatcaat gctgcaagct ttcccttaat gctgtaacac cagtaagagt tgtcaagacc
      661 agcagactga ctaggtaaat accgctcaaa aacaagatta cggcagttaa actataatga
      721 tccatcaaga aaccgatgac gatagaagaa aaaccaccaa cagctcgtcc aaggttcata
      781 atgaaattat tcgccgtcgc gcggatttct gttggataca aactactaat cactgcgcca
      841 tatccaccat acatgccatt gataaagtag ccgactatca cagcggctgc taccaaactc
      901 cactggctat gggctaacac aagtagatag acagataagg atgaacaaat taaaaacaaa
      961 gaaaaggcta gtttaggccc caatttatcc atgattatgc caaaagtcat catccccaaa
     1021 gacatgccta aaatagtact gaccatccac aatgaagacc cagaaaccga aagccctaat
     1081 cgattttgca taattgaagg cagccaattc attaacccaa aatagccggc aatttggaca
     1141 gttaccatga tactaaggcc acatgtttgc caagcaagat gttttgattc aaacagtctg
     1201 ctaagtttgg ttttcttttc ggcagattgt acagatttga attcctttgg ttcagtcaag
     1261 ccgcgacgaa tgaacaagac catcaacacc ggcagtacac ctacgacata caacatcttc
     1321 caaccaaacg ttggaatgat cacagcagct aaaatcgcag ccaaagccgc accgacctgc
     1381 gcaccaatac cagcaaccga agaagctcgt ccaatttgtt tttttgaaaa actttcggaa
     1441 atcaaggaca tacacgcccc gtattctcct cccgctccta cacctgcgat aaagcgaaag
     1501 agataaacca agtagatgtt tgaagcaaaa tacatcaata aagaagcaat cgaaaacaga
     1561 aggaccgttt gagaaaatac tttcacacgt ccatatttat cagctatgat gccaaaaaaa
     1621 atacctccaa tgagcatccc caaattagtg atcgttgaaa tgaaacccgc ttgcgtacca
     1681 ctaatatgaa aagaagtaat cattgaagat aatgaaaaag ctaaaaacat aatatccata
     1741 ttctctaaag caattcccgc cgatgtcgat aataatacct ttcgctgata cgttgtcatt
     1801 tttatacccc ttttcctaaa a
//
LOCUS       DBKOIJ010000267         1797 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000267 DBKOIJ010000000
VERSION     DBKOIJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.275"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(46..291)
                     /locus_tag="ACV5NJ_002846"
     CDS             complement(46..291)
                     /locus_tag="ACV5NJ_002846"
                     /inference="COORDINATES: protein motif:HMM:NF024059.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486860.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(285..686)
                     /locus_tag="ACV5NJ_002847"
     CDS             complement(285..686)
                     /locus_tag="ACV5NJ_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HHY6486861.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(873..>1797)
                     /locus_tag="ACV5NJ_002848"
     CDS             complement(873..>1797)
                     /locus_tag="ACV5NJ_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HHY6486862.1"
                     /translation="KGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKTVIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     VCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILRVQGNK
                     KGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          562 a          350 c          235 g          650 t
ORIGIN      
        1 agaagattct tttaaacaga aaaagtggaa aggggatttt tttaattagt taattttaaa
       61 ttttaagatc gcttctatta atttaatttc tagctgactt atcatatatt catctacata
      121 ggtaacgaat tgacctacct cgttaaatcc atttgtcaaa cataacttag ccatataaga
      181 atgataatgt ttgagtatca tcttgatcga aattggatct ccttctagag cagataagat
      241 aatggatagt ggaactaata ggtactgccg ctttttagat ttatctacca taggtcatac
      301 cccattttca attgttcaac tgccttcttt cgttgatacc agataccact tgtgctcata
      361 ttgagaattt taccgatttc agcatcatta aaacctgcaa aataataaag taggataatc
      421 tttcttctta tctccgataa ctgatcaatt gctgctgata acttttgatc tgaaattaat
      481 atttccattc caaataagaa atagacatct tgttcatcaa aatgagtttg ggaatacctg
      541 ccttcatttg aatctgccaa tggttcctca ttatccttta tgttagcgtt tcgttttcga
      601 atagacttag cttcgtttcg taatactttt ttacagtagg catcaaattg gtgttgtata
      661 gatatgtcat tgtaatgttg ttgcatgata atatgctcct ttttccccct ttccactaat
      721 acaacaatca gaaagcccct ttttttcgaa aaaactagaa aaaaaacata aaaatattta
      781 tcattcttta aacagaaaat ctttctataa aaaacaaaaa aaaacgatta ggctaggcta
      841 atcgtttttt gaacattctt aaaaaatcca aattattttt tataactgaa attgggtatt
      901 gatgaccatc gtttctgtag atagtaagca atcattttag gttttctatc tctagtaaag
      961 attcctttct tatttccttg cactcttaaa atcccttgac ttgttgcaaa atctgcaaaa
     1021 ttccacactt gttcaccaac aaaattttca catgtatcta ttacttcgtg atttgcttcg
     1081 taataagcca cttgatactc ttctgtaaac attgttggta ctgtatcatg aaatccagct
     1141 actgtatctg ctccatattc tgtaaacatt attggcgtat caggacatct ttccatccat
     1201 ccatctagct cttttttcaa cagatatttt gcatgtgcca gatcccctcc cgaaacatac
     1261 caaccataat aacgatttaa acataaaaca tcgacaatat ctcctacctt acaaacatct
     1321 ggcgttgatt ttagataagt aacgattgtt acaggtcttt tctgcggatc aagttcttta
     1381 gttaattcaa ctaaaggttc aaaatattct ttagcacctt cggaatccga atctggttca
     1441 tttgcaatag accacattac aacacttggg tggttcttgt cgcgttctat caattccttg
     1501 atcaccgttt tatgagaatt ttctgttcgt atctctttcc acgtattatg tttttccaca
     1561 ttatcatcta ataaagtggc catgaaattt aaatgtaacc ctactgcagg tacttcatca
     1621 ataactacaa taccttctcg atctgctaat cgcataattt cttctgagta aggataatgt
     1681 gatgttctaa aagaattagc acctatccat ttcattaaat taaaatcttt aatatttaca
     1741 gcctcattca aaccacgtcc attaacatac gaatcctcat gcttaccaaa tccttta
//
LOCUS       DBKOIJ010000268         1796 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000268 DBKOIJ010000000
VERSION     DBKOIJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.276"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..189
                     /locus_tag="ACV5NJ_002849"
     CDS             <1..189
                     /locus_tag="ACV5NJ_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HHY6486863.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1480)
                     /locus_tag="ACV5NJ_002850"
     CDS             complement(1055..1480)
                     /locus_tag="ACV5NJ_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HHY6486864.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
     gene            complement(1582..>1796)
                     /locus_tag="ACV5NJ_002851"
     CDS             complement(1582..>1796)
                     /locus_tag="ACV5NJ_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HHY6486865.1"
                     /translation="YENIKKPKIWSSIVYGGKIAFYFSAIIEFLQLFLRLGTFQISDF
                     VYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          568 a          351 c          212 g          665 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatt
//
LOCUS       DBKOIJ010000269         1780 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000269 DBKOIJ010000000
VERSION     DBKOIJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.277"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(226..1692)
                     /locus_tag="ACV5NJ_002852"
     CDS             complement(226..1692)
                     /locus_tag="ACV5NJ_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HHY6486866.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          564 a          359 c          312 g          545 t
ORIGIN      
        1 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aatggtgttc
       61 aatcagctga ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc tatttttgtt
      121 tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc
      181 cagaagtagc ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac
      241 tgtttctggt tcagtaattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat
      301 acctaataag atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc
      361 tttaggatca gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt
      421 gaaggcaaag caccaagcac caaagataaa accgattttt ggatttttaa caaatttata
      481 ttcagaattg aatttcttat aagctttatt caacatcata tatgccaaga acacccatag
      541 gtaacgcatt ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat
      601 atctttgata ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag
      661 ataaccattc accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc
      721 tggatccgca tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga
      781 gatttgtcca atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata
      841 gtaattacct aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttagggat
      901 attatcgcta gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac
      961 cattgctgct aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt
     1021 gacataaggg gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt
     1081 gaaataactg aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc
     1141 aaattccatc gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga
     1201 catgacgaac atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt
     1261 tgttgataaa tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac
     1321 tgacatcgta tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat
     1381 tgcttgaggc ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata
     1441 gtatgccagt cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact
     1501 atccttgaag gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc
     1561 caaaatcaat agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac
     1621 attccccatg ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg
     1681 tttgttgttc attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat
     1741 tcaattttta acaaacttac aagacagtgt tattttatgt
//
LOCUS       DBKOIJ010000270         1775 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000270 DBKOIJ010000000
VERSION     DBKOIJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.278"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(185..1633)
                     /gene="cls"
                     /locus_tag="ACV5NJ_002853"
     CDS             complement(185..1633)
                     /gene="cls"
                     /locus_tag="ACV5NJ_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HHY6486867.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT          535 a          383 c          295 g          562 t
ORIGIN      
        1 gtgtctcttt taatagcata tattagctta tttcttgctt tccccaaaaa acaagagata
       61 ccccaaagtt aatttcgctc atgcgagacg ctttctccat ttctgacgta ctctcagaaa
      121 tggccccgtt agaaaaggac acgagatctc catctgttga tggttctcat gtccttttta
      181 tgttttaaag gagcggcgaa atcaatcttg ctaatccttc tttcatcttg atgattattc
      241 ctcggttttc atacatttct tttgtgagat gcgtagaaat tttcgagtct tcaaaaaaag
      301 cttttcttac acgtgaagcc attcgttcat cataaaccag tgtattgacc tcaaagtcca
      361 agcgaaatga acgaacatcg atatttgctg agccaactga aacaatgcca ccatcgataa
      421 tcatcgtctt tgcatgaata aatccattct catatgtttc gatccttgct ccatactcga
      481 tcagctctgc agcgaaagaa tacgttgccc agtaaacgag aagatgatct ggtttgttag
      541 gtatttggat atgaactttt acgcctgaaa gaagagccaa cttcagtgct tcatgaatcg
      601 atccatccgg tatgtaataa ggcgtttgga tcaatatctc ccgtttggct aacgaaatca
      661 ttttcaaata agtcattttg atttgttcat gttcggtatc tggtccgctt gtcacgattt
      721 gaacagctat atcaccagta gtgtgaatgg acgggaaaaa tttgggagaa tagcctactt
      781 cttttgtatg ctgagaattc cagtccatca aaaaacggtt ttgcaaggta tacaccgctt
      841 ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa tttctttacc aatcctagat
      901 attcatttcc aacattgaac ccacctgtat atccgatttt cccatcaata actacgattt
      961 tacgatgatt gcgataattg atccgaggat taatatacgg aacaaataga ggaaagaaaa
     1021 atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa gctggaagaa acttgtgtgg
     1081 aaccccatgc atcaagaagg actctgactt ttacccctcg tcttgccgcc tctgtcaatg
     1141 catctcgaac ttcttcacct aatgcatcac tgcgataaat ataatactgg aagtggatat
     1201 ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa tttttcacga ccatctgtaa
     1261 ataaagtgat ttcattttta gtcgtataga gcgtactttc aaataccgtg agcatataga
     1321 tcaattgttt cacatctact tgtccagtag gcggatgagg ataaagccct cgcatcaatg
     1381 cctgtttgtc ttcttcaatt tccaggttct taccgatttt cccttggatc ttcaaatcaa
     1441 agattttctc tttggaaatc cctcttccta aaaaaatata taaaataaat cctacaactg
     1501 gaatgaacat caaaacgagc agccatgccc atgtttgagc agtttccttt ctttctcgaa
     1561 aaacgatgat aaaggaaagc aaaatattca taatcaaaag gatcgtaaaa aaattgtcaa
     1621 cgataatttt cacggcttca cctcttcttc taccaactta agaataccca agttgttttt
     1681 taaagtaaag ggaaaaaagc cgcttccttc tattctccta gccaataaaa aaagaaagct
     1741 ggaacgaaac tgtccaactt tctcatttta tttat
//
LOCUS       DBKOIJ010000271         1766 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000271 DBKOIJ010000000
VERSION     DBKOIJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.279"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            139..690
                     /gene="lepB"
                     /locus_tag="ACV5NJ_002854"
     CDS             139..690
                     /gene="lepB"
                     /locus_tag="ACV5NJ_002854"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HHY6486868.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(773..1315)
                     /locus_tag="ACV5NJ_002855"
     CDS             complement(773..1315)
                     /locus_tag="ACV5NJ_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HHY6486869.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1552..>1766)
                     /locus_tag="ACV5NJ_002856"
     CDS             complement(1552..>1766)
                     /locus_tag="ACV5NJ_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HHY6486870.1"
                     /translation="KEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHEVDMG
                     MPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT          621 a          300 c          302 g          543 t
ORIGIN      
        1 actttatgat caaaaaagtt ttttgtccaa tagcctaatt gaaagaatta tttttatttc
       61 agctatctag tcgcattttt ttgatatttt tcttataata aagaaagaat gaaaaaaaga
      121 gggatggatt gatagagatt gacaaaaaaa caacgctata ttaatcgctt ctggcttgta
      181 ttcaaatact tacttgtttc tgtattcctt gcgttcatgt tgcggggatt tttattcatc
      241 ccggtaccag tggaaggcaa ttcaatggaa aacgtgttga agcaaggtga tatggttgtg
      301 atggaaaaat tttcggagat cagacgattt gatattgttg tttttcaatt agcagatggc
      361 acgatttata tcaaacgagt aatcggtctt ccaggagaaa atgtcagcta ccaaaatgat
      421 caattgaaga tcaatgggaa agtagttaag gaaccttatc taactaaaaa cctgaaatca
      481 gatcatgcaa atgcatccta tacgacggac tttacactac aagagctaac gggacaatcc
      541 aagcttccgg aagataatta ttttgtactt ggagataatc gaagagtttc aaaagatagt
      601 cgttcgtttg gcacgatcaa taaaactgat attctaggta aagcaagatt tgtctattat
      661 ccacttgatg aaataaaatg gatccagtga gaaacaataa agcgtgcagt ctgttgagac
      721 tgcacgcttt attgtttaat ccaaaatcag tttactacct tagtacgtaa aatcaaacag
      781 ctcgtttaaa cacttttcta gcctctaaag ttttgataaa tgaataaaca accattattt
      841 gaatagggat ttggatcata ttcctgatga atctacctag aaaaattact ttgaaatctg
      901 caccaaacaa aacagacatc agccaagaat tgataaaaag gctgactggg atcataatca
      961 ataattgggc agcgatgacg gaaggcaggt ttatcttttt atgatataaa aataagccat
     1021 taataaatcc accaaggaaa gccgtgataa gaaaagcagc ttgaaacgtt cctgaaccaa
     1081 atatcgtgaa atttaaagtg ttcgacaaca tttcaagcaa accagcccag atcggtccat
     1141 atagataacc cataagcgcc atacttaaaa atccgaatgt gatttgaaaa taagcaggac
     1201 caatcgctat cttgtataag atgacatcaa gggctaataa aagagcagag gtcgacaaca
     1261 atcttgcatt catttcgggc aatctaaaaa cgtcttttgc aatcaaagtg ttcaaaatat
     1321 tctctccttt gtcagttaat aataaaagga taccataaaa tcaccaatgt gtcatgacac
     1381 attggtgatt tacatacact ggcatctaac aattaaaaaa agggtgagaa gactgctttt
     1441 ataaactgta aaaaagagat tgtacgaata aaaaaaatag aggcggagaa tacttatcgt
     1501 atcatccgcc tttgtttttt tgttataaaa aacgtcagat tagttatctg cttacatctt
     1561 catccatacg ggttctaatg tatttccgtc tagatcttgg acttctaatg aaaacatttc
     1621 ttcttcaggc attcccatat ctacttcgtg gaaagtacca ccattttctt ttgcaatttg
     1681 agcaaatttt tttacttcat ccgcattttc aacactaaac gaaatcaaag caccacttgt
     1741 ttttttatta tcagctattt ctttat
//
LOCUS       DBKOIJ010000272         1664 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000272 DBKOIJ010000000
VERSION     DBKOIJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.280"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            131..505
                     /locus_tag="ACV5NJ_002857"
     CDS             131..505
                     /locus_tag="ACV5NJ_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HHY6486871.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACV5NJ_002858"
     CDS             complement(619..1248)
                     /locus_tag="ACV5NJ_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HHY6486872.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACV5NJ_002859"
     tRNA            complement(1382..1454)
                     /locus_tag="ACV5NJ_002859"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DBKOIJ010000273         1660 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000273 DBKOIJ010000000
VERSION     DBKOIJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.281"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     regulatory      96..262
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            436..1542
                     /locus_tag="ACV5NJ_002860"
     CDS             436..1542
                     /locus_tag="ACV5NJ_002860"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HHY6486873.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          479 a          270 c          349 g          562 t
ORIGIN      
        1 aaatattaaa gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag
       61 gaaaagtcta tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc
      121 tatacaaaca taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac
      181 tgttggatta aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa
      241 aactcggacg atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt
      301 tctttatatt tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt
      361 ggattgctgt gtgaggagga gatgcggtga aaaaagatcg attggtggat tttgggtcct
      421 tatttgactt tgtccatgat cggtctttta gaagtataca gtgccagttc ttatcgttta
      481 ttgcaagccg atgaaaatac aaaaagtcta ttattgcgtc aactaatttt catatttttg
      541 agttggagcg tgatcttctt agctcgttca gtcaaactac actatttact tcaccctaaa
      601 atagcaggat acggtttagc cttatcgatt ttctttttag tattagtaag aatagggata
      661 ttcggtgtca ctgtcaacgg cgcacaacgt tggatctctc tgtttggcat tcaattccag
      721 ccttctgaac tggcaaatct ttttttgatt ttttatttaa gctggttttt tcgtgacgga
      781 aatagtagcc caaaagatct aaaaaaacca ttcctgatta cagtaggtat aacttttctg
      841 attttatttc agccaaagat tgctggagca ttgatgatcc tttcgattgc gtgggtcata
      901 ttttgggcag cggcggttcc atttaaaaaa gggatctatc taatcgttac tttttctgca
      961 ttgctgattg gagcagcagg cggggtatta tatttaggaa ataaaggttg gcttccacag
     1021 atgtttaatc atgcttatga aaggatcgcg actttaagag attcttttat cgatagtcat
     1081 ggtgcgggct atcaaatgac ccattcgttt tatgcactat ataatggtgg catatttggg
     1141 cggggattag gaaatagtat cacaaaaaaa gggtatcttc cagagacaga aacagatttt
     1201 attttttcga ttattacaga agagcttggg ctaatcggtg cattgtgtgt gcttttctta
     1261 ttgttctcat tatgcatgag aatcttttgt ttaagcagtc gctgtaaaaa tcagcaagct
     1321 ggattgtttc ttttaggatt cggaactttg ctattcgttc agacgataat gaacgtggga
     1381 agtatcgcgg ggctgatgcc aatgactggt gtgccattgc catttgttag ctacggagga
     1441 acaagttatc tgatcctttc tttaggaatc ggaatcacgt tgaatatttc ttcaaagata
     1501 caggccgaag agttggcgct ctatcgacct gaaaaacaat aaagagaaac tgagaaactc
     1561 tcagtttgaa ttatcataaa gtacaatctt ttatttttca agcgagaggt tcttttagaa
     1621 agaggaattc gcaatgaaag tatctcaaaa aatcatatta
//
LOCUS       DBKOIJ010000274         1645 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000274 DBKOIJ010000000
VERSION     DBKOIJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.282"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(60..284)
                     /locus_tag="ACV5NJ_002861"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACV5NJ_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACV5NJ_002862"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACV5NJ_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          582 a          244 c          217 g          602 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatct
//
LOCUS       DBKOIJ010000275         1587 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000275 DBKOIJ010000000
VERSION     DBKOIJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.283"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            96..1574
                     /locus_tag="ACV5NJ_002863"
     CDS             96..1574
                     /locus_tag="ACV5NJ_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486874.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          575 a          219 c          291 g          502 t
ORIGIN      
        1 aagcataatc catcataaaa tcagtatagg tgaaaagatg aactttcatc tgcatatcaa
       61 gtaattggta tgaggcattt tttgaggtgt aggatatgaa gttggattac cttatagaag
      121 aggaagatag agaaaaactg ctgctagcta gatatttaga atttgagaaa aagaattatg
      181 tagtgctgcc taagttatat gaaacattag gtttgtctaa atttaaaaca agaaattata
      241 tcatagaatt aaataaagaa tttactgaat tcaaaacaaa gccagagatc caaatttcag
      301 aagaagcaga gatccatgtc gaacgtctga atttgacgga tatcaaaaat ttaagaagaa
      361 tctactttca acgatcaaaa attgctcgac tgttaacgga gatcgtggat tcaaaagtaa
      421 cgatcgaaaa atttgcagag aatcattttt taagcatatc aagagcctat gtaaaaagaa
      481 gagaattagt ctcttttctt gaacagcatc aaataaagtt aaagaaaaac atattagttg
      541 gggaagaaat aaacatacgg aacgtattat ttggggtata tttcggggtc tttaatggtt
      601 tttctatgcc atttagagaa agcgtcatga atgaagcaaa gagtatcgtg caatattttg
      661 aatatctgtt caatttaaaa ctgtctgcaa caaaacgaat caaattcaat ttgctaacca
      721 ctattagttt gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc ttttcagatg
      781 aatactttaa gagctcagaa ggaatgaaga tcatcaagga tttatcacat atttcttttc
      841 ttcatatccc taaagagcag atcattgatg agatttctta tatcatgttg tttttgatca
      901 cggaagaaga atttgaatta gatggcgcat ttggacttgc ggaagctcat tttcatgata
      961 tccacatgat tagtcaaaaa atgtcagtgg aattgattga tcaattacca ttttcgattg
     1021 aaatctctga agaagaaaga ctatatatac gaaaagaact tcgtatgggt atgatgcatg
     1081 tcaatcgaaa aaattggata ttcgactttg agatgactag ctttacgtct aaaaagcagc
     1141 tacagttttt cttcgagacg tatccaatat tcagtacagc aatccaaaag gtcatacaag
     1201 agaataaacg ctatttcttg ggaaaagatg aacgtttcat gattcgctta ttttatgatt
     1261 atctcttttt attggtcggt atttgtccgg tatcatattt tgaagcaccg attcatgtct
     1321 gtattgattt ttcgcaagga tctgcttata ccgattacat tatttctcaa gtgaaaggat
     1381 ttaaaaatta caatattgtg attgagagtc gaatcacttc tcgtacgaat atttatttat
     1441 ctgattgttt acttgaaaaa tttaataaag aacagataat ttggaaaaat ccaccgactc
     1501 cagatgactg ggaatatttt ggaaatacca tcatcagaga aaaaaataga catttaactg
     1561 aaaaaatcag ctagtattta tggatgg
//
LOCUS       DBKOIJ010000276         1565 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000276 DBKOIJ010000000
VERSION     DBKOIJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.284"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            320..652
                     /locus_tag="ACV5NJ_002864"
     CDS             320..652
                     /locus_tag="ACV5NJ_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6486875.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            562..756
                     /locus_tag="ACV5NJ_002865"
     CDS             562..756
                     /locus_tag="ACV5NJ_002865"
                     /inference="COORDINATES: protein motif:HMM:NF024673.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHY6486876.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            795..941
                     /locus_tag="ACV5NJ_002866"
     CDS             795..941
                     /locus_tag="ACV5NJ_002866"
                     /inference="COORDINATES: protein motif:HMM:NF012870.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486877.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1379..>1565
                     /locus_tag="ACV5NJ_002867"
     CDS             1379..>1565
                     /locus_tag="ACV5NJ_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HHY6486878.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFAFDGTLTY
                     QPEECFHRHYQNEQTIIK"
BASE COUNT          553 a          286 c          277 g          449 t
ORIGIN      
        1 agtctcactc ctaaaattac tttatttacg tatgaagtgg gcaggaggtc gaatccagaa
       61 ctgggctatg gtacttcaac gaggacttct cctctagaat tgaaaagcat gcgatttacc
      121 ttccgtaacg acggtagatt tattcaacta tgtgtccaat caaaatttgt ctgatttcaa
      181 aaatagtcaa gagaattttg gttgttaaaa actcttatct atcaacttcc acagtttagt
      241 tgacaaaccc tgatactaag ttgggctatg atacactttt cttgactatc agttacgtta
      301 ccggaaggaa tgaactctat tgagaaaaac atatgatcgt aaatttaaac taaagatttc
      361 acaggatatc cttgagaaaa aagtaactac caagaaaatt gctgaagaat acaatatctc
      421 tcgtccaaca atttcccgat gggtttcaga atatcgtcga tacgggaaaa atgcttttgc
      481 tggacaagga aaaagattac ctgataaagc agatttctac attcttgaac aggaaaataa
      541 acgactaaaa gaggaagacg agtgttctac gaacacaaag atcgatacgg atctccaggg
      601 atcactcaaa aactttacga tgaaggaatc gaaatcaata aacgtgttgt agctgttctt
      661 atgcgtgaaa tgaatttatg cacaagagga tttcatcgac gcaaatcttc tcatggtaga
      721 gaaaaaacaa tcaaagaaat ggtaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa
      781 ttgatgctgt ttgggtgatg gatattactt acattccatg taatgacggt cgcctctatt
      841 tgtccaccta tattgactta gcaactcgta ttctacgtta ctatatggta gattcacaca
      901 tgaaaaaaga gattgtcgtc cgtccattac aaatttatta aggagaattg cctacggtaa
      961 ttcattccga tagaggtagc cagcatcggt cttttattta tcaagagctg ttgaacgacc
     1021 accacatcac acattcagtg agccaaactg ggactcctgt tgacaatgca gtaattgaag
     1081 cgtttcatcg atcaatcaaa tgagaattaa tcgactccaa caaatataaa agtaaaatag
     1141 agatgaaggt gttaatccaa gattatttag aagactacta cccaaacaaa agaatccata
     1201 caaaatttat gatgactcct tgacaatttg aagcgaagaa aataagggat ctataatatc
     1261 tagtgttggt gacgccttct gttgccaata ctttttcttt tttctaataa acgcaaacag
     1321 acaccttaaa atcctttaga ataaagtcga caaaaaccat tcaaagtagt gtttcaagat
     1381 gtcctattca gagtttacca aagagttact agatattcta gacctaaatc ttacctttca
     1441 tgaggatgcc tttcgcaaag agcgcatcaa tgacgaaaca tgttttgctt ttgatggcac
     1501 attgacctat caaccggagg aatgctttca tcgccactat caaaatgaac aaacgatcat
     1561 taaat
//
LOCUS       DBKOIJ010000277         1515 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000277 DBKOIJ010000000
VERSION     DBKOIJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.285"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            5..1471
                     /locus_tag="ACV5NJ_002868"
     CDS             5..1471
                     /locus_tag="ACV5NJ_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HHY6486879.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          415 a          289 c          320 g          491 t
ORIGIN      
        1 gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat tgtcaacttt
       61 gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat tacttatcta
      121 ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa cagcattagc
      181 tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg gatgggtatc
      241 tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca ttatgcttgg
      301 ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga tggcattgat
      361 tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac atctttattc
      421 aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg tcaacttagg
      481 ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact atcatttagg
      541 gttctcttta gctgctattg ggatgttctt tggattatta caatacatca tccaaggacg
      601 caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg aagaacgtaa
      661 aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat tcggcggggc
      721 acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg tattggggat
      781 tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa ctgctgaaga
      841 aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt tctggtcatt
      901 acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac aatctacgct
      961 atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt tcatctttat
     1021 tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac catctacagt
     1081 agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat tgatgttgcc
     1141 gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg gaagtttctt
     1201 catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga caacaaaatt
     1261 agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg atgcttcttc
     1321 acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat ctgcttactt
     1381 cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg tgaaagaacc
     1441 aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag gaattgctta
     1501 tttgcagcag ttcct
//
LOCUS       DBKOIJ010000278         1482 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000278 DBKOIJ010000000
VERSION     DBKOIJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.286"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..484
                     /locus_tag="ACV5NJ_002869"
     CDS             <1..484
                     /locus_tag="ACV5NJ_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HHY6486880.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1215)
                     /locus_tag="ACV5NJ_002870"
                     /pseudo
     CDS             complement(691..1215)
                     /locus_tag="ACV5NJ_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1319..>1482
                     /locus_tag="ACV5NJ_002871"
     CDS             1319..>1482
                     /locus_tag="ACV5NJ_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HHY6486881.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTG"
BASE COUNT          482 a          277 c          287 g          436 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atccgagagt gtaaaatatt ttgtgtaaat agaaaaaagg
     1261 aagtcccttc agtagaatag agttaccaca acacattcac agaaaagagg acttccatat
     1321 gaacgatttt actacagaaa ttctaaagac tctagcgaat aaaggcgatt tgaatgaatt
     1381 attccgtgtc catttggaaa aagctgtcaa tacgcttctc aaaacggagt taacggcttt
     1441 cctcgattac gaaaagtacg atcgcattgg ttttaacacg gg
//
LOCUS       DBKOIJ010000279         1445 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000279 DBKOIJ010000000
VERSION     DBKOIJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.287"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            476..727
                     /locus_tag="ACV5NJ_002872"
     CDS             476..727
                     /locus_tag="ACV5NJ_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486882.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            761..1039
                     /locus_tag="ACV5NJ_002873"
     CDS             761..1039
                     /locus_tag="ACV5NJ_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486883.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
BASE COUNT          514 a          197 c          267 g          467 t
ORIGIN      
        1 atacaaataa ttggtttatt ctggcaggcg taatgctttt ttctagtttc tatttattta
       61 gtaatgctct attagcacag cgagggaaat ataagtttaa aatattagat tagatattat
      121 ttttagaggg gataaatata aaatgaaaag aggttattta taatggaaaa agtattacca
      181 attaagagct actgtattcc aaggcttttc atcatagaat ttcaaaagta aagacatgtg
      241 tttcacaaat agtgtctatc ctggctaaat aattcttcca ctatctgctt taatttcagt
      301 agacttttcg gcaagttatt tgctaatgag atgaatagag attacaactc gcctataacc
      361 gcttaaaaca agctttttta aagtatagtt agcaagtcgt attcatatgt tcaaagagga
      421 gggcttatga actttgaatt cattccgtgt gaacagaaca gtagggggaa gtaaaatgaa
      481 agaaaagcct ataaataaat ggtcattgta taaaaattca atgtttggag ttgttatctt
      541 tggtgtttgt gctggattat tccttttcat attattacaa acggtgactc ttttctcttc
      601 taatcctact tatggatgga gagacagtat agccttaggg atgtttgcgg gaggatatgg
      661 attcttattg ttgttctcaa ttctcacact aagtccaatc catggactca ttcaactttg
      721 gaagtaagaa cgagccttaa acttttcgtt ctcggaagaa atggaaaaac acaacataac
      781 aggcagtatg tatgtggatg atcaatggtt tatcagcact gatgaggtaa aaatagctgc
      841 cattaggaga gattacctgc agaaaattca taaaataaaa gatcctaatg ggaaaaaggc
      901 aaggttaatt ataactttga taacatctaa tggtaagaag ttaaaacttg caggatcata
      961 tgataatgtt aaaaagttcg aacattggta tctgcaaaaa gacagcatta gtcttaataa
     1021 acaatttgaa aaaaattaat caaagtcatc ggcaacacga ataatttgac atcttgatat
     1081 tgtgatataa gcaaaaacgg catttctgag accgaacagc ataaaaaatt taaatgttgg
     1141 aatttatata agcaaaaccc aagaagctag atgcgataga gcatttaatt tcttggggtt
     1201 ttgcttggga atcagcatat caattaaaat gatcatctcg gtatttcaaa agtgggtacg
     1261 attacaaata gttggagcaa aatagtgggt aaaattacca ttccagtgtc tgtcttgcct
     1321 ctttttcgtc aatcacaaat agacgagatt cataggaaaa atcgatacag ggtatgattt
     1381 caatgatatt atgaccaatt acaaaataga ttgataaagc attttttgca tttttataat
     1441 agttt
//
LOCUS       DBKOIJ010000280         1445 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000280 DBKOIJ010000000
VERSION     DBKOIJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.288"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            27..>1445
                     /locus_tag="ACV5NJ_002874"
     CDS             27..>1445
                     /locus_tag="ACV5NJ_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486884.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGF"
BASE COUNT          583 a          204 c          214 g          444 t
ORIGIN      
        1 catacaaata aaaaaaggag atttatatgt ttatattaga tgatgaaata aaaaaagtta
       61 aaaatcctag aattattcca ttattagaag aagttagaag tagtttttat cagggaaatt
      121 atcgttctgc aatagctgtc aattacagtg cgcttatttt agatttaatt gataaattat
      181 ctgatttagc aaatatttac gaggataata cttctaagaa aattttagaa gatatagcta
      241 gactaagaaa aagtgatcca aactctccaa aatgggaaat ggaactacta gaaaaagctt
      301 atagttcaac tgagctaatt gatagctatg aatatgagga tttaaaatat attaaggatc
      361 aaagaaatta ttcagctcat cctgtagtca cttacaacgg taatacttgg gaattaagag
      421 atataacaaa agaaacttgt caggatgtta tacggaagtc ctatgaaatt atattcctaa
      481 aaaatccaat attaggaaaa aaagtaacga tcgaaattat acaatatgca agtcgcatac
      541 atgctatgtc tgttacacct gaagaatttc actctgcact aaaaaatagc tatctaaata
      601 aactaagcga aaaagccaaa gaaaacctat gtaagacagc ctttaagttt gtttttcaat
      661 tatcttatgg aaatgaggaa gcagatagag atagggaaag tactttcaga ctacttaatg
      721 ccctaatttt tgataataaa gaattctatt taaatattct aaaaaaagaa attgcaaagt
      781 ataggttcac agctgaatca agagaggaaa ttaataaatc acttggtgaa aatcaacaga
      841 tcacgtatac aaaagggttc tttttactta cttttatagc tacccatcca gaattacaac
      901 atgatttttc agacgagaca attcaaaatt tacaaatatc aataaatgat tttaaaccga
      961 aacaacaagt actaacagaa atagaatatt actaccgttt acgttccctt gcagttctag
     1021 gaaacaacaa tgaagaatta caaaatcact taaatagtct agttgatcct atactccagc
     1081 aaaaaatgag agtttcttcg ctaccatctt caacattaga aacaatatat aatcaatatt
     1141 tatattttgg tgaaaaagag atgttcatag attttatgat cgaacatctt accgatgcta
     1201 gttcattcag gttagcagat aaggatatgg aagtattacc atggatttac aataaattaa
     1261 atcaggagca atttaacaaa ttactataca acctaaatgg aaataatcaa ttttacaaaa
     1321 acagtaattt tccgcctttt atttctaatc tgttagactt ttataatcag aaattccaag
     1381 tgaatttacg taaacattat agtgggcttc tatatccaaa tgcagtgact atggatcagg
     1441 gtttt
//
LOCUS       DBKOIJ010000281         1417 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000281 DBKOIJ010000000
VERSION     DBKOIJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.289"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(150..1337)
                     /locus_tag="ACV5NJ_002875"
     CDS             complement(150..1337)
                     /locus_tag="ACV5NJ_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HHY6486885.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DBKOIJ010000282         1411 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000282 DBKOIJ010000000
VERSION     DBKOIJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.290"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(252..1277)
                     /locus_tag="ACV5NJ_002876"
     CDS             complement(252..1277)
                     /locus_tag="ACV5NJ_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HHY6486886.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          419 a          232 c          278 g          482 t
ORIGIN      
        1 aataagggta aatgtgttta aaaaaacgaa ctataaaaac ttatacacaa cgtggttaaa
       61 aatagttttt tcttccctgt ggaaaaaata gtttaacaca aacattatag ttatccacag
      121 ctatctaaaa cagatggaaa ataagaaaaa ttcgctaaaa taccgggagg gggtaacgag
      181 ggatgagtga ttgaaaaaag aacagagtaa ctgacttatc ctcagtgttt ctgttctttt
      241 ataattctgt tctacatttc ttgttgttgg atcgaatctg tgagagtaat ctcggttgaa
      301 agaatgattc ttttttcttc agggtattgg tggtggatta gttggaataa aagttctgtg
      361 gctttgattc ctaactgatt tgtcggaatg tctatccgag tcaactgtgg cgaaaatagg
      421 gaggacaata agggatcact gaatcctgcg atcgaaagat catcaggtat ctgctttcca
      481 gattgttctg ccactttgta aatactcaat gattttaatg catcggtaga aaaaatcgca
      541 gttggtcttt ctcgtagctt cattaaatca ttcattgctg ccatgctatc ttctaagcga
      601 aagccgccgt ctataatcca ttcattacgg atatctaggt ggtgatcaaa taagcattga
      661 cggtaaccat tgagacgatc attggataca taaacttctg tcggagcatg gagacagccg
      721 atatgacggt gtccattctt gatcagtgtt tccaccaaat cgtaactatc ctggaaatta
      781 tccgtgtcca ctgagtagat gttcgtgtag tcgtttccga cttttccgat taccacaata
      841 ggaatattgt attgatccaa ttcaaaaaga agtgtttcat ccatcgtaga gcttaaaata
      901 ataattcctc ggatcatttt ttcttggatc tttttcgcgc atttttctag ctcttcttct
      961 ggtgtttttg ccgtttgtag aatcaagtcg aagttatttt gttcagaaac ggcagaaatg
     1021 gattgaataa catctgaaaa aaaaggattg ccataggtcg tatgagaagt acgatgagat
     1081 aatgctagaa tcgcgtcaaa accatttcct gacagtcctc ttgcaatttt gctcggttca
     1141 taattcaatt ctttgattgc ttgaagtaca cttgcttttg ctttatcaga gatatttggc
     1201 tggttattta atactctgga gacagttgat tttgagacat tagctttttt cgcaatgtca
     1261 taaatagtag gtttcaattt tatatgcacc tcctttttgg gagcgtttta gaataattcc
     1321 atagtacaat agacattctt ttttgtctat gattttgaaa atttaggaat agcttgcatg
     1381 atcatgagag accatatttt tttaagaagc t
//
LOCUS       DBKOIJ010000283         1411 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000283 DBKOIJ010000000
VERSION     DBKOIJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.291"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..690
                     /locus_tag="ACV5NJ_002877"
     CDS             <1..690
                     /locus_tag="ACV5NJ_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HHY6486887.1"
                     /translation="IYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFI
                     TLFQPFMIIWVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLIT
                     AMINIVLNIILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLI
                     SLCVIAFVASYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYI
                     LKVLTSVLVKR"
     gene            868..1089
                     /locus_tag="ACV5NJ_002878"
     CDS             868..1089
                     /locus_tag="ACV5NJ_002878"
                     /inference="COORDINATES: protein motif:HMM:NF012360.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HHY6486888.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          481 a          137 c          289 g          504 t
ORIGIN      
        1 atttatgaat catgtatagc aggtataggt aacagtctta ttgtagaaag tcaagaaaaa
       61 aattatatag atttttgtga tattacaaca attataggtt ggtttctggc aattagttgt
      121 gcatgcttta ttacattatt tcagccgttc atgattatct gggtgggaag tgataatctt
      181 atgagtattt catttgttat atttatgata gttcattttt tcttatatga aattgatcag
      241 ttaattgggt tatacaaaga tgctgctgga atatggtacg cagacaggtt tagaccatta
      301 attacagcaa tgataaacat tgtattaaat atcatcctcg ttaattggtt cggattatat
      361 ggagttttgg cggcaaccat tgtttcagta ttgttggttg atttaccctg gttattatat
      421 aatgttttcc aaaatatatt taaatttgaa tcggcaggta aatatgtttg gaagctcata
      481 agtttgtgtg taatagcttt tgttgcttct tatttaagtt attttgcttg ttcgtatgtt
      541 aatatcggaa atttcaaagt gataagttta gtgataaatt tagttatatc ggttgtcata
      601 tcgattttag ttattcttct gtttaatatc cgtaataaga agtttaagta tattttgaaa
      661 gttcttacgt cggtattggt aaaaagatga gaagagacat cagaatgata tataaagtaa
      721 ctacaatttt taggctgccg aataggcgaa cgatgtatag tttttgttcc aacgaagact
      781 cttgttgaaa ttggagatga tatgaaggag ttacaatttt gactcatgtt tatgattgat
      841 cggttttaaa agtagtatgg agaagctttg ggatcatcgg agaggatcaa aataggtaat
      901 aatgttttta taggaatgaa gtctagaata ctaaagggtg tacatatagg aaataatgta
      961 gttataggtg caaatagttt agtaaacaag gacgtatcag acgattgtgt tgttgcagga
     1021 aatctagcaa aagtaattat gtccctagat gaatattata aaaagataaa caatatgaag
     1081 aatccgtaaa acttgtgaaa ctataccgag aataatatgg gaaaaatatt gatgaaaaag
     1141 cattatatga gtttttaggg ctgtttacag atggagatga caatttgtcg aagagttggg
     1201 agcaatgcaa aatttagtag gggattataa acagactgac aaggtattta aagaacatgc
     1261 acaagtattt cataatattg aagaattttt gaatactgtg agataggagt tgctacattg
     1321 agaaaagtat gtatttttcc aatgtaaaag attacgagtt tgaaaacgac accataatat
     1381 tattttttaa gaagatatta tagagcttag g
//
LOCUS       DBKOIJ010000284         1401 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000284 DBKOIJ010000000
VERSION     DBKOIJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.292"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            80..1024
                     /locus_tag="ACV5NJ_002879"
     CDS             80..1024
                     /locus_tag="ACV5NJ_002879"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HHY6486889.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          506 a          206 c          276 g          413 t
ORIGIN      
        1 aacatgttga taaaatgaat aaacaagact atacttttca tgaaagtaac aaatagttat
       61 gaaaaggagc tgtttattca tgaaaaaaac aagtcgtaaa gtagtaatcg tcggtacagg
      121 ttttgttgga acaagtatcg cttatgcaat gatcaaccaa ggaatctcta atgaattagt
      181 attgatcgat gtgaatcaag aaaaagcaga aggggaagca ttggaccttt tagatggtat
      241 ggcttggggc gatgaaaatg tggctgtatg gtcaggtggc tatgaagaat gtaaagatgc
      301 tgatatcgtt gttatcacag ctggtatcaa ccaaaaacct ggtcaatctc gtttagattt
      361 agttaaaaca aatgcgtcaa tcatgcgcca aatcgtaaaa gaaatcatgg gatcaggatt
      421 cgacggtatt atcgttgttg cttcaaaccc agtagatatt ttgacatata tcgcttggaa
      481 cgaatctggt ctgccaacat cacgtgttat cggtactgga acaacattgg atacaactcg
      541 tttccgtaaa gaaatcgcat tgaaattaaa agttgaccca cgcagcgtcc acggctatat
      601 cttaggtgaa catggtgatt cagaagttgc tgcatggtca catacaactg tcggtggtaa
      661 accagtgttt gaaattgtag aaaaagacca tcgtattgcc caagatgaat tagatgtgat
      721 tgctgataaa gtccgtaatg ctgcttatga gatcattgat cgtaaaaaag caacttacta
      781 tggtattggt atgagtactg cacgtatcgt aaaagctatc ttgaataacg aacaagctgt
      841 attgcctgta tctgcttatt taactggtga atacgacgaa aaagatatct ttacaggtgt
      901 gccatcaatc gttgacgaaa atggtgttcg tgaagttgtt gaattatcaa tcaacgaaga
      961 agaaaaagca atgttcagca aatcaacaag tgctttaaga gaagtattga acactgtatt
     1021 ataagaaaca atgaatatga actaaaaatc ctaaaactgc ttaacagctg aaatagctgt
     1081 taagcagttt tttattgata tctaataggt aagcaaataa atagaatggt taaaattaat
     1141 cggtccgaaa atgtatccgt acatatgaac atatgaaatt taatctgttg attacaataa
     1201 gcgaatcgtt atcaatgttt atctgtggtt aattaaaaaa tccttccgca acggcaaata
     1261 caatgattca tatctaattc gttcatattt gattaactga ataaagaaac tggtttttat
     1321 cattcaatta aacgacaaaa aaagtaagaa aaactgcaaa atttttttat aattttgcat
     1381 aagcagattt tgatggaaaa a
//
LOCUS       DBKOIJ010000285         1388 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000285 DBKOIJ010000000
VERSION     DBKOIJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.293"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            154..417
                     /locus_tag="ACV5NJ_002880"
     CDS             154..417
                     /locus_tag="ACV5NJ_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HHY6486890.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="ACV5NJ_002881"
     CDS             417..689
                     /locus_tag="ACV5NJ_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HHY6486891.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="ACV5NJ_002882"
     CDS             766..1095
                     /locus_tag="ACV5NJ_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HHY6486892.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1085..1371
                     /locus_tag="ACV5NJ_002883"
                     /pseudo
     CDS             1085..1371
                     /locus_tag="ACV5NJ_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          525 a          169 c          277 g          417 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa acatgtcatt aagtatttaa aaagctaaaa
     1141 gagaagccat tgaaagaaaa atttttgaat ttaatttatg atgaaattgc gattgatcca
     1201 tataagggtg aacaaaaaac tggggattta tcaggtattt ggtctagtgg gttcaaatat
     1261 gcgggcacaa catatagagt agcttacgag attatagaaa acaaggttat tccagtattg
     1321 ttatgtggaa cgcatgaaaa cttttatgaa cagttgaaaa aattaatcta gtgatttgtt
     1381 gtcgttct
//
LOCUS       DBKOIJ010000286         1373 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000286 DBKOIJ010000000
VERSION     DBKOIJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.294"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1007
                     /locus_tag="ACV5NJ_002884"
     CDS             <1..1007
                     /locus_tag="ACV5NJ_002884"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HHY6486893.1"
                     /translation="IIPIRVRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQA
                     VLMAGQYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKA
                     REFGAMTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGS
                     KKWETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAA
                     CWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSN
                     KLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY
                     "
     gene            989..>1373
                     /locus_tag="ACV5NJ_002885"
     CDS             989..>1373
                     /locus_tag="ACV5NJ_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HHY6486894.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEE"
BASE COUNT          486 a          185 c          262 g          440 t
ORIGIN      
        1 ttataatacc tattagagtg agggataaag aaatgagaca actacacaaa gaacagattg
       61 aaacagcaat ggcagcgtta aaggaaaaga caattgatca tgtatatttt acagcatgtg
      121 ggggttcaca agccgtttta atggctggtc aatatctatt tgataaagaa ttaactgtac
      181 cttgtcatgt ttatactgct aacgaattta actatgctat accaaaagga ttatctgaaa
      241 attctttagt tatcacttgt tcccattctg gcacaacacc agaaactgta gaagcaacaa
      301 aaaaagcaag agaatttgga gcaatgacta tttgtttatc taatgaagaa ggatctcctc
      361 tatgggaagc agctgaatat cctgttcact atgattgggg taaagatgtt gatgctagtg
      421 atttaaataa aggaattctt tatggtttat tatttaatat tttacttgtt ttagatggct
      481 ctaaaaaatg ggaaacttgc ttgaatgaat ttgataagtt aaatgatctt gttattgctg
      541 aaaagagcaa atttgaaata actgctaaag aatggggtaa aaaccaaaaa cgagataaaa
      601 ttatttacac aattggtagc ggtattaatt acggagaaat ttactccaca gcagcttgct
      661 ggttcatgga aatgcaatgg attaattcta atgctattca ttctggggaa tttttccatg
      721 gtccttttga aatcactgat tatgatgtac cattcatctt agtgaaaagt attggagata
      781 caagattttt agatcaacgg gtagaagact tcactaaaaa attcagtaat aaattaatgg
      841 ttttagacca agctgatttt gacttagaat ctatagatag tgtagcgaaa gaatatattg
      901 cagctgtact tagtggcgtt gttattcgcc aaatggtaga agcgattgct tttgaaagag
      961 gccattcctt agatgttaga cgctatatgt ggcaaatgaa atattaatta ataggaggtt
     1021 gcaaaagatg gcaaatcaat tatttcacat tgtgattaca acacattcta ccctttgtga
     1081 gggttattta aaggcaacaa aattaatttt aatgaatgac gccacaggta ttagtgctgt
     1141 tccatttaaa gaaaatacta gtccagatga ttttgaggag agtatgaaag aaataattag
     1201 tcagcatcat ttgcaacctc taattatttt aacggattta attggaggaa cacctaataa
     1261 tgtttctatt aaacagttga acaacaatag aattcaaatt attgctggga ttaatttacc
     1321 tcttttactg gagttattaa tggcccagtc agctggtaaa tctatagaag aaa
//
LOCUS       DBKOIJ010000287         1364 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000287 DBKOIJ010000000
VERSION     DBKOIJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.295"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACV5NJ_002886"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACV5NJ_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HHY6486895.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DBKOIJ010000288         1362 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000288 DBKOIJ010000000
VERSION     DBKOIJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.296"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            82..>1362
                     /locus_tag="ACV5NJ_002887"
     CDS             82..>1362
                     /locus_tag="ACV5NJ_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HHY6486896.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DBKOIJ010000289         1356 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000289 DBKOIJ010000000
VERSION     DBKOIJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.297"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(323..937)
                     /locus_tag="ACV5NJ_002888"
     CDS             complement(323..937)
                     /locus_tag="ACV5NJ_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HHY6486897.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          440 a          268 c          227 g          421 t
ORIGIN      
        1 tggggaagac tctttcatca acgtgtatta agagacaaga aagaatccat atggattctt
       61 tcttgtctct ttttccacta ctttttattt ttctgacact acatattctt ataactcatt
      121 tctcataact gtgaaacaac ttataaaaat aacaaacttt aaacaaaaca agcttaaaat
      181 caaagatgat ccatcttaaa ataactaaaa caaaaaaagt agtcgttgtt aatcctcatt
      241 aataatcgtt ttcttttttt caaacgctcc atttttatgt ggaacattat ttttttaagg
      301 aactttgtca acatgtggat aactattttc cacttgtgtc ctggactagc tgttttgctg
      361 taaaatacaa aacttgctgc agcaaaaaga tcgttgcaag agaggctgtt ccgcagatcg
      421 ctggatctag atttggcaaa gtcaagactg catcatgtaa attggcgtgg ttgtccaaaa
      481 ccaaatcagc aaattcaaat aatcgcaaac cactgctatg ttgcgatttg atacttcgtg
      541 acaaatcaaa tccagtaaca atgattaatg gatgaccatt ttctttgatc cactgagcta
      601 gttcgatgat tgccgggttt cttccttcat tcgaaagaag aagaaaaatg tcttctgatt
      661 tgacagaaag ctgatcaata attgcctgtc ctgcaccttc tagcgtttca tagacacctc
      721 cggctggatt ttcaatcaat atacttggta caagatccgg ggtttgatcc caaaattcaa
      781 atgctacacc acttagtgtg cggctagcat atatctgcaa tctaccacct tgattaattt
      841 gtttcgcgat ccaagtgcca gcttgaagca tcagcggctc ttctatcctt aatttctctt
      901 ccaattggtg ataatagtca aaataacata aactcattta tctctcccgc ctagctatac
      961 ttgacatgaa tagaaacagc tgcctaatct aaggaagtac ataaaaaaac agtagttaga
     1021 cagtaacaca tgtggtcttt gctgtttcaa acacaaaaaa ggcattgaac gaatacgccc
     1081 aataccttga catagcaaat aaaacaacgt gcagaccatg gaaaatatct acataggggt
     1141 agaattttca ccctagatta ggtacaataa attaggtcga tgcactgcat tgactatggc
     1201 aaaatgatcg gtcagggtgt atgtgtgtga ctggtcattc cctgattctt agcgccaact
     1261 aagggtcagg gagccttttt ttattcagct gtttctatct gaatttattg taagtaatct
     1321 cttatccaag tgcaagcaag aaaatacaaa aaaaca
//
LOCUS       DBKOIJ010000290         1350 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000290 DBKOIJ010000000
VERSION     DBKOIJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.298"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            1..988
                     /locus_tag="ACV5NJ_002889"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACV5NJ_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          472 a          239 c          267 g          372 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaatagca tacggaaaga agttctgcac
     1081 cttataaaat ttgtcaaaaa aactgttgcc attccaatga gaaaaagcat ggaatgcgct
     1141 gcatcaggta tcgaggctta gaaaaaaatc aggtgcatac gatgcttgtt tttgccgaaa
     1201 tgagtctgaa aaaattggtc acttagcagt gggagcatat gtcttcatat tttttattat
     1261 acaaaaatag acaaaagtat cagtctacac tcttagtaaa gaggaaactg atacttttgt
     1321 ctacagtatg atatttctta catctacttt
//
LOCUS       DBKOIJ010000291         1344 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000291 DBKOIJ010000000
VERSION     DBKOIJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.299"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..614)
                     /locus_tag="ACV5NJ_002890"
     CDS             complement(<1..614)
                     /locus_tag="ACV5NJ_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HHY6486898.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKF"
     gene            965..1264
                     /locus_tag="ACV5NJ_002891"
     CDS             965..1264
                     /locus_tag="ACV5NJ_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HHY6486899.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          489 a          193 c          220 g          442 t
ORIGIN      
        1 aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt
       61 tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca
      121 gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa
      181 cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt
      241 gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa
      301 ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg
      361 ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc
      421 attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt
      481 ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca
      541 aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact
      601 ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa
      661 ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg
      721 ctatctcttg aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat
      781 aaaaatctta gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc
      841 gttgccataa cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag
      901 atgtagcgta tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat
      961 cgtgatgtct aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt
     1021 aaatccagag atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga
     1081 aaaaggtcag tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc
     1141 aatcagggga gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg
     1201 aaaatttgaa aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt
     1261 ataaaaacga gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc
     1321 taggttcatt tatttgaaca gagt
//
LOCUS       DBKOIJ010000292         1294 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000292 DBKOIJ010000000
VERSION     DBKOIJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.300"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1244
                     /gene="tnpC"
                     /locus_tag="ACV5NJ_002892"
     CDS             <1..1244
                     /gene="tnpC"
                     /locus_tag="ACV5NJ_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HHY6486900.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DBKOIJ010000293         1273 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000293 DBKOIJ010000000
VERSION     DBKOIJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.301"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(121..204)
                     /locus_tag="ACV5NJ_002893"
     tRNA            complement(121..204)
                     /locus_tag="ACV5NJ_002893"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Leu,seq:cag)
     gene            732..1232
                     /locus_tag="ACV5NJ_002894"
     CDS             732..1232
                     /locus_tag="ACV5NJ_002894"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HHY6486901.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          410 a          217 c          213 g          433 t
ORIGIN      
        1 tttttgattt atttgaatct ggttccgttg tttaaaattt ttcctatatt cttttagata
       61 atatgttatt ttctttctca tcgctccagg tgtaccttct ctttataaaa aaacaaatta
      121 tgcggctaag aagactcgaa cttccacgag gtcaccctca ctagcgcctg aagctagcgc
      181 gtctgccaat tccgccatag ccgcatcaca tcttttagtt taggattaca tgtgtttttt
      241 gtcaagaaca tatttttgtt ctttttcatt tctgtacgcc ctcccttatg ccatctacac
      301 aaataattca tccgagcaac tccatcatct attgagggtt ggtgaaaaaa tctgttttat
      361 ccatatgatc gatttcaatg ttaaacgaac gactctggga ttagtaacta ttttattttt
      421 ttcttcattt cttttttcct ctttttctcg attcagctat cttctttcat ttggatattg
      481 attatttctt ctgttgatta ctatccttct ggaattctag acgctgaagt aattcacgtt
      541 ttctttcttc tagagatcat aagtaacgaa tgaacgaact tcaaataatt tttttattgt
      601 aattcatatt tgtattgttc gatgattctt aatacgatat gacacaataa ataaaaatag
      661 gcatggctat ttacaacaat agtgctatag tagaaaacaa atgaataagg ttacttaaag
      721 aggtgaatac gatgaaactg atcaaactat gggaagcaga tttaaagaaa gcttatcagc
      781 tacaaactag ctttggacca aatgagagtg gatttatcaa caatgcttat ggctatacat
      841 ttgctgaatt tttagattat gtaaaaaaat gcgaaaaaac gtctttagga atcgatcttc
      901 ctgagagtta cgttcccgca accgtcttta ttttagaaag tgatgaacat gagtatgtag
      961 gaatttttaa tttgcgccat aaactgaacg aaacattaag aaacggcgct ggccatattg
     1021 gttacggcat ctcccctaat tatcgtaaaa aaggatatgc cacacaagga ttagcattag
     1081 tactacaaga ggcaaaaaaa atcggcattg atgaggcact tttgtctgta aataaaaaaa
     1141 atcttggcag tcttaaagca caacaaaaaa atggggctgc gatttatcgt gaagatgaga
     1201 aagaatacta tacgaaaatc aaactgatgt aaaaaagaag gattccaaaa ggaaagggat
     1261 ttgaatgtcc ttt
//
LOCUS       DBKOIJ010000294         1255 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000294 DBKOIJ010000000
VERSION     DBKOIJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.302"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            251..565
                     /locus_tag="ACV5NJ_002895"
                     /pseudo
     CDS             251..565
                     /locus_tag="ACV5NJ_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(870..>1255)
                     /locus_tag="ACV5NJ_002896"
     CDS             complement(870..>1255)
                     /locus_tag="ACV5NJ_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HHY6486902.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          460 a          171 c          220 g          404 t
ORIGIN      
        1 catttttttc aatattagct tactagagaa taagaaaaag taagtaataa accaaattta
       61 aactttatat tataataatg aagcttaatc ttctttatag agaattgggc tatttttaat
      121 ggcaaagagg ggaagaagat gtttcaaaac aaaggacaac gctatgttgt aaaaggagta
      181 aaggaatctt actggaatca aaaatcttta ttgagaaaat tatctttgaa tttaagcaca
      241 aagaaatgaa ggtacactag atagcaatga agatatcttt gtcattaaac gtcgctattt
      301 tacacaagta agtgatgatt tgattcatgt cttttccaat gagtggtttg tttctgagaa
      361 aaaattacat gcttctaatc tgcaatatat gccaggtgaa gatccaattc ctaatatgaa
      421 agcaattatc aacagtaagg attacgaagg atataaagcc aaacatccag aagcgaaacc
      481 atttaaatac ccacaagaaa tgaagcgagc atggcgtaaa atgttagatg atgagctgat
      541 tccccttgaa aatgaattga ggtaaacgag aagaattcaa taaactggat gataaaaata
      601 taatatgact ataaattggt tacagcttca acctataagt tgtaataaat tagcctaaaa
      661 gtaatttatt aatgatgagg tgaatttgat tggtacaaca agatatatca cttagagagg
      721 gagaagcagc actacaaact acttttcaat aagtaggaat tatagaaatt ttactcaaaa
      781 aatcttcagt aaacaatttg gctaaataag attcagttgt tatacgagaa ggttacggtt
      841 cttcttaact aagcttagta tgtttttatt caaggcatga ttttctctat gtgatcattc
      901 agtttctgta agagttctaa taattctttt ttggagtact tgtaattgct ttctacccac
      961 cgattgagaa cggatagaca accttggacg tggtacgtag taaagaatct catttcttct
     1021 gtgataatcg ttttcttgtc ttcatatagc catatgttca gatagttttc ttcaataact
     1081 tgtttaactt tcttctgaaa cgaaatatca ccatggttga ggataaataa gcgatataaa
     1141 aaagtatttg cgtaaacata gtcaatcaag cgagtgtata gttcctcata gctatgggct
     1201 ggatcataag agagaatttc atttagatcc cgaaaaaagt tgctttctaa ttgat
//
LOCUS       DBKOIJ010000295         1231 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000295 DBKOIJ010000000
VERSION     DBKOIJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.303"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>1231
                     /locus_tag="ACV5NJ_002897"
     rRNA            <1..>1231
                     /locus_tag="ACV5NJ_002897"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DBKOIJ010000296         1228 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000296 DBKOIJ010000000
VERSION     DBKOIJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.304"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..1195)
                     /locus_tag="ACV5NJ_002898"
     CDS             complement(<1..1195)
                     /locus_tag="ACV5NJ_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HHY6486903.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLI"
BASE COUNT          437 a          228 c          163 g          400 t
ORIGIN      
        1 taattaattg aatcaaatgt ttaattcctt gttgaatatt atcaagcgta accgtacgaa
       61 atgatttttt cattaattta acaaattcat ggtctatacg gttatatact ttctcttcac
      121 cgctatatgc tttaattgtt tctattcctt taagactttc tatgatacta gaatttacag
      181 ctgcaccggc tgccatttct tccctgttcg atttttcata gcttttcacg aaagcgaata
      241 ttgcaactac ataaaaaggc aaagagcata gagtgattaa aaacaacgta ctattctgaa
      301 tagctaacgt aaccccaaca agcaacacca taccaatatc taaaaatatt gataacgtag
      361 cacttgctaa agcatcaata atcttactgg catcaagaaa acgagaaata atttcccctg
      421 attttctcgt agcaaaaaaa ttcattggta aagttaacac atgtttgaaa tactgaagca
      481 taattgccat actcattcgc tgtcctaaaa taataagaag ataactacga ctatattcaa
      541 acaatacccg aaataaatag acagcgatca agccaatgga aattatattt aaagttgaac
      601 gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa ataatatgag cttcctatgc
      661 caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa tactaatcct ttttgtttcc
      721 aaattatagg caaaaaagaa cttaatccag ctactttttc tttaatagga atataagttt
      781 ctctaggcat aggtaataaa agaatacctg tccattcttt ttgaaacgct tcaatcgttt
      841 ttctattttt tccattagcg ggatctgcaa tccatagtac gtttcccttc gttttataaa
      901 ttacaacgta gtgcatatag ctgttatcaa ccaatatatg tgcaattaat ggtaagataa
      961 gatctctttc tttccagatc tcactatctg cttgaatagc catacaatca aaagacaatt
     1021 tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc taaatcagtc ccagataggt
     1081 cccgtaattt atgaatcggg atatcagttt taaaatattt taaaatcatc gaaaggcaag
     1141 caacaccaca atctttttca tcttgttgct gcacaaaagg aaatgatttt atcacttatc
     1201 taacacaact ccttctaaaa aattttta
//
LOCUS       DBKOIJ010000297         1222 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000297 DBKOIJ010000000
VERSION     DBKOIJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.305"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..359)
                     /locus_tag="ACV5NJ_002899"
     CDS             complement(<1..359)
                     /locus_tag="ACV5NJ_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HHY6486904.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFP"
     gene            complement(512..838)
                     /locus_tag="ACV5NJ_002900"
     CDS             complement(512..838)
                     /locus_tag="ACV5NJ_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486905.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(850..1050)
                     /locus_tag="ACV5NJ_002901"
     CDS             complement(850..1050)
                     /locus_tag="ACV5NJ_002901"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486906.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
BASE COUNT          362 a          202 c          135 g          523 t
ORIGIN      
        1 gggaacattt gcattttact tttattgaag ccattttttc ttttcacaag aagtttaact
       61 ttttgaagtt catctagtgc tgataagatt gtattttttg acattcccga aatttctacg
      121 agatttttga tttcaaatat caaaaaaatt ttttgattct catcatacaa gttatttttc
      181 aatgaaagtc tatttctatc ttttagtaac atataaacta acttagaact tagtttcatt
      241 ttcttatatt tttcatttac gtttaaaaaa ataggatatt gatagtataa ttctgcatat
      301 atatcagaat tagaatagcc ttgcttttca actaagcttt caacttcttt ttcattcatt
      361 gtcacacctg cttctgattt tatttgctat aatagattca aataaagttt tctcagaact
      421 ttattattga gtgttccccc gatctgcaaa ctgacgggaa cactcttttt aattttttct
      481 acttcatcaa atttaaaaaa cgaggtgttt tttaaatttg atgactcaat atccattttt
      541 tgcattcttc ttttgaaaac aactccattt tttctaataa aataactggc aatcccattc
      601 ttttaaattt caaaaaagtt gtatatgata cattcaaata tttagccatc tcttttcctt
      661 ttagaaatgg tctttctaat ccggtatcaa ttcttgcttg atcaatagcc tcttttgtca
      721 tttcaaaaac ataatttttc aattgattcg tttgttgttt atctaacatt actgtaattc
      781 ctgcatccat tatttttctc ctctcatttt ctatttcttc aatcagccaa ctcgccaagt
      841 tgtatttatt tagattttca aatcattaag taaccaattt accaactttt gataagtaga
      901 tgctctaata ctttctttgc tcccattttc gattttgaca tatgttttcc gatttatacc
      961 tatcaatttt gctgcatcgt ttttagtaag tataagatcg gctctatgtc ttcttaagct
     1021 tctaatgaat tgatcattaa gagttatcat tcagcgcctc ctttcaaatt attcactttg
     1081 tggtttttat cttaataagc acattttaaa ccacaaaacg agtaatatca attaaaatat
     1141 tactcaattt gtggttttca tgctataata gtttggaatt tactataggg ggcgttactt
     1201 tgaatagaat aaaattttat at
//
LOCUS       DBKOIJ010000298         1217 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000298 DBKOIJ010000000
VERSION     DBKOIJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.306"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..164)
                     /locus_tag="ACV5NJ_002902"
     CDS             complement(<1..164)
                     /locus_tag="ACV5NJ_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HHY6486907.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTG"
     gene            194..604
                     /locus_tag="ACV5NJ_002903"
     CDS             194..604
                     /locus_tag="ACV5NJ_002903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486908.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            685..>1217
                     /locus_tag="ACV5NJ_002904"
     CDS             685..>1217
                     /locus_tag="ACV5NJ_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486909.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIES"
BASE COUNT          445 a          162 c          216 g          394 t
ORIGIN      
        1 cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt taactccgtt
       61 ttgagaagcg tattgacagc tttttccaaa tggacacgga ataattcatt caaatcgcct
      121 ttgttcgcta gagtctttag aatttctgta gtaaaatcgt tcatatggaa gtcctctttt
      181 ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt ctatttacac
      241 aaaatatttt acactctcgt caaatataaa aactagtacg aaattaattt gtaaattatt
      301 taagcttgat aaaataagca atatcgatga tgtaaagtcc aagatattaa caagaaaagt
      361 aagcgaacca gtaaagctaa cggcacaaga taaaaaaaat ggaattgttc tttatggtga
      421 tgatgcaaag tataactata attgggatac ctcctcagaa gctgaggtat taagtattcc
      481 tactaccgat acttcagggg aaaaagtcac ctttaaaata aattttataa cggcaatttc
      541 taagttggag tatgggaagc aaaaaaagag agataacaat gatcaatatc tacctaaaaa
      601 ataacgaatt aatgatgcat atgttaaagt tgtttttttg aattaaacaa ctcaatttat
      661 tcagttatat gttcatcgca gcaagtgaac attaatcgag ttataaaatt aataggagct
      721 aaaaatataa ataaagttcg atcagaattt atttttccag aagatttttt taactggttt
      781 ttttatcaat tcagtgaatg caagcgactc ttaggtgaga attattactt aaaaggaatc
      841 ggcggattta tggggaatgt ggctgatgaa caaaatatta taaaaggaat atctgaacaa
      901 acatcagagc ttacagtaac taaagcattt ataagtaatg gtgaaagttt gaaaaatgta
      961 actgcgcgaa ttagaaaaga aaatgaagta gacattgttt ttggaattga ccatgaatgt
     1021 aattcagtga tatatgtaac acaatcagca aatttaattc tttttgagaa tggttgcaag
     1081 gacgatttta tggtggtcta tttgtacgat atactaattc caaaacttcg ttccttatat
     1141 atgtcttctg aaagtatttt tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt
     1201 attgaagtaa ttgaatc
//
LOCUS       DBKOIJ010000299         1215 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000299 DBKOIJ010000000
VERSION     DBKOIJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.307"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            414..947
                     /locus_tag="ACV5NJ_002905"
     CDS             414..947
                     /locus_tag="ACV5NJ_002905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HHY6486910.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DBKOIJ010000300         1197 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000300 DBKOIJ010000000
VERSION     DBKOIJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.308"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(561..>1197)
                     /locus_tag="ACV5NJ_002906"
     CDS             complement(561..>1197)
                     /locus_tag="ACV5NJ_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HHY6486911.1"
                     /translation="QSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDV
                     QAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQV
                     TNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPML
                     TRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
BASE COUNT          394 a          203 c          166 g          434 t
ORIGIN      
        1 tatcttttat taaagtcaga taaaaattct ttggaaatgt aatggtagag atatttttac
       61 aaaaggactt gcagcgctgt agttggtgct gcaggaggac ctatttcggc cgttacagga
      121 ggtattggta atgtacttgt cggatgtgca ttaaagcctg ttcctgcaag ttaaatttat
      181 aaaaacagga aagtaattat ctcaaatcct atcaagtaca atcataggat tgcacttgac
      241 aggacaaatt ttttaactaa aaaatgaaaa acactttcta aaaattagta gaatttgcca
      301 aatctttcgc atcttccacc atacatttaa ttttaattcc cctttatggt acttaataaa
      361 aacaagaaaa gaactattca tttggccctt ttttggttcc agaccataga aatattcctc
      421 cagtaactaa tattgctgtt atgcacgtaa ctaataacag ctataaaaat tggtacaact
      481 ttaataggat tcagaaaaat ttgcactagt gaaggaatct attttattag aaaaatgcaa
      541 tctccctaaa gaaaaaacca ctaaacagtg ctttctttca acttctcctt aattttttgt
      601 gctttcccta aagtaaagtt tctggccaac tctgaatcaa aatatagtga acgtttttta
      661 aaatctattt ctttaacgtt aaagatattt actaaacacg ctcgactaat tcttgttaac
      721 ataggatatt tcttttcaag ctcactaatt cttccataaa attcgtactg cccatcttta
      781 gtacacaatg ataatctatg tggtaatttt gaagcttcta caaaataaat gtcatttata
      841 tcaaaagtaa atgtttgaga acctatggaa aaaacaaatg cttgattcgt aacctgtttt
      901 gttgcatcta tcctttcttg cgctaataac aataactcaa caatctcgtt tcgatactca
      961 tctagtgttt gatcttttgt tacaaatcct aacgtttcaa ctcttctttt tattgttacg
     1021 ggtaacatct catcatgagt agttgtaaaa atgatttttg cttgaacatc atattttctg
     1081 attacttctg ctaattctat accgttaact tcatgattta aatcaatatc taagaaatat
     1141 attccttgct ttggtcggaa ttgtttgagg tattttttaa cttctaacgg actttgt
//
LOCUS       DBKOIJ010000301         1191 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000301 DBKOIJ010000000
VERSION     DBKOIJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.309"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            18..491
                     /locus_tag="ACV5NJ_002907"
     CDS             18..491
                     /locus_tag="ACV5NJ_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HHY6486912.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            500..727
                     /locus_tag="ACV5NJ_002908"
     CDS             500..727
                     /locus_tag="ACV5NJ_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486913.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          376 a          192 c          202 g          421 t
ORIGIN      
        1 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
       61 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
      121 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
      181 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
      241 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
      301 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
      361 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
      421 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
      481 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
      541 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
      601 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
      661 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
      721 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
      781 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
      841 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
      901 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
      961 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     1021 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     1081 tgttaactct tactccattc ttttttagta agctcccata tatactgtaa agccttctat
     1141 atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat g
//
LOCUS       DBKOIJ010000302         1148 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000302 DBKOIJ010000000
VERSION     DBKOIJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.310"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            44..454
                     /locus_tag="ACV5NJ_002909"
     CDS             44..454
                     /locus_tag="ACV5NJ_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HHY6486914.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            468..824
                     /locus_tag="ACV5NJ_002910"
     CDS             468..824
                     /locus_tag="ACV5NJ_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HHY6486915.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          407 a          167 c          219 g          355 t
ORIGIN      
        1 aaattactgt agaattggat tataattaaa aaggagtgag aaaatgataa aagtatatac
       61 ttcgcctagc tgtacttcct gtagaaaagc aagagcatgg ttgcaagcga atcaacttga
      121 atttgaagag aagaatattt ttgccgaacc gttaacagaa agtgagatca aaaagatctt
      181 ggcagcgact gaaggaggag ttcaagatat tatttccact cggtcgaaaa tttacgaaaa
      241 gctgggcata gattttaacg aattaacttt aaagcaaatg atcgtcttat tcaaagaata
      301 tccttcatta ttgagacgac ctattttgat tgatgaaaaa agaatactaa tcggatttaa
      361 tgaagatgag atccggtcat ttgtccctcg tgaaattcga tgtgttgcca gaaatctgat
      421 ctatttacaa aacaataaca tcttaaattc atagggaggg agccttcgtg gaagtgaaag
      481 tctatggatc agcgcactgt gtaacaagtc tgaaggcgaa acaatggtta gaacaccatc
      541 aattgcctta tcaatttgtt gacttagaag aacgcgatct aagtacagaa gaagcagaag
      601 aattatgcag ttttgaagaa gtgcaagcag aacagctatt tgcgacttgg tctgaggctt
      661 ttcacaacat tgcgattgac ctttcctgtg taggaaaaaa acaactgatt tttttatgca
      721 cacaacatac caatctcctc agacgtcctt tgattattat cgatgaagtt ctatttattg
      781 gatacaatga gcagttgatg gaacagctta tcttaaataa atagacttta actgttttaa
      841 tcaagtcact gagcatttca tacagttgta acagtcgaac acttagcatc aagcgtgtta
      901 tgtgttcttt ttttgacttt atttttctac tatagagaca tgctcaattt atgtggtcaa
      961 aattcaaatc taggtgggga tatagagtta aaaatatatt gacatgtgta aatttacatg
     1021 atatattcat ttctgttgtt ttaagtagca aagcaataaa atagtatttt aaaaaacata
     1081 taaaatagtt gttgacatgt tagaaatgac atgataagat aactgagttg ttaaaaaaga
     1141 accacttg
//
LOCUS       DBKOIJ010000303         1148 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000303 DBKOIJ010000000
VERSION     DBKOIJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.311"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACV5NJ_002911"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACV5NJ_002911"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HHY6486916.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT          320 a          247 c          203 g          378 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacga
//
LOCUS       DBKOIJ010000304         1134 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000304 DBKOIJ010000000
VERSION     DBKOIJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.312"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..251
                     /locus_tag="ACV5NJ_002912"
     CDS             <1..251
                     /locus_tag="ACV5NJ_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486917.1"
                     /translation="TIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHDVDRN
                     EILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(613..852)
                     /locus_tag="ACV5NJ_002913"
     CDS             complement(613..852)
                     /locus_tag="ACV5NJ_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486918.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          396 a          162 c          210 g          366 t
ORIGIN      
        1 aaactatcat tttaggaaaa aaatatattt gccaacctat tggcttgaaa caaaaagtgg
       61 ttggagaagt aatcagtaaa ttagaaaatt gtgttgtgat ctgcgtagac gaatatcatg
      121 acgtcgatcg caatgaaata ttagaaaaat gcggaaaagt agtggcaaaa tatactcacg
      181 tttatgagtt ggaaacaaca gaatatctat ataactatag caacaaatat gaacatactt
      241 tagtttcata aagatttaaa taaagtgcct tcaaaagcga gaagaccaag cttttgaagg
      301 cactttattt agttggcagt gattgttcat gatttttgat ttattcaaaa ggttcgccta
      361 gagaatctgt ctctgtaacg aggcctggtg gagaaactcg ataaaaacct atactgccag
      421 aactgtccta ttttcggata gacaaactag tagttttaaa aagatcatcc gatccaatca
      481 tttcaaaaaa tgcaaaactg atatcaggat catttagctc aggcttgtta tcgtaaagca
      541 tttgtaaagg ttgcatttag ttaggagtaa acgcgaaact atcatcatta tttgctgaag
      601 aagatgaaga agctaattca gagtttgttt gaaataaagg agacgtgctg acagtttctt
      661 taccctgttt tttgcagagg tagaaacttt tatatcggaa gtttctaccg gagaattttt
      721 ctcatttgta gaaattgttg aatcgatact attcgaaatt tgcgtatctg aaggtatgtt
      781 ctttgatgca gaagtcccac aagcagtaag taacaataaa tttgataaac tgataaacag
      841 aattcttttc attatgtaca cactccttat agtatcgttt agcatacacc tatgctcagt
      901 attaggcaaa atcaatgcaa aactatcaag tcttttatag tatctattag tcttagtaat
      961 acatatatgg agagggtgat atcccagttc aaaccagata cattggttac catctgtatt
     1021 aacgattatg cctctaaacg ctttttttat gaaaaaattt ataagtgaaa acgtaggtat
     1081 gatattatct agaggtaatt tttttgggta tttaaatagt tgctaggagg atag
//
LOCUS       DBKOIJ010000305         1124 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000305 DBKOIJ010000000
VERSION     DBKOIJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.314"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>1124
                     /locus_tag="ACV5NJ_002914"
     CDS             <1..>1124
                     /locus_tag="ACV5NJ_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HHY6486919.1"
                     /translation="YKPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQ
                     RFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYR
                     VMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPY
                     LERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNH
                     RVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIA
                     DRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFL
                     PQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          336 a          229 c          227 g          332 t
ORIGIN      
        1 tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt
       61 gggggacgac aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg
      121 agctgaagaa acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct
      181 ctgttgctga aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct
      241 taaaagagaa tacttccatg acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg
      301 tctatcgtgt catgaagcga ttctaccgcc cattgaatcc tttcaaacag actttgccaa
      361 aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta
      421 tcatgatgga cggacaaagc catgcattat tggatattgt cgaaaatcga cgcttgcctt
      481 atttagaacg ctatttttct cggttttcat tagcgactcg tgaagccgtt caattgattg
      541 ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt
      601 tgatcatcga tcgtttccac atcgtccaac atattggcag aactttcttg aatcatcgag
      661 tgaaagaaac cacggtacga cttaaaggaa attcccactc tcaacgaggg ttagggaaaa
      721 aattgaagcg ctattggaaa ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac
      781 gggtttggcg tgccagtttt aaagattatt tgactgaaag tgagattgcc gatcgactgt
      841 tagctgcttg tcccaactta cgccaaggct atcagttata ccaagacatt ctttatgcgg
      901 taaagaaaag agatcagtca ctatttgagg attgtttaac tagagacatt agcggtctgc
      961 cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg
     1021 cgcttcacta cagctattca aacggaccat tggaatgctt aaacaaccac atcaaagtat
     1081 taaaacgcaa tgcgtacggc tttcgcaact tttacaattt caaa
//
LOCUS       DBKOIJ010000306         1113 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000306 DBKOIJ010000000
VERSION     DBKOIJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.315"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            3..968
                     /locus_tag="ACV5NJ_002915"
     CDS             3..968
                     /locus_tag="ACV5NJ_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HHY6486920.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT          283 a          219 c          191 g          420 t
ORIGIN      
        1 atatgaagaa ctttttgaaa attattccga tcccgatctg cgggttgatc cttgggttag
       61 cttcccttgg aaatctgttt aaagactatc accatgttgc tttaggcaat ctaactggcg
      121 gtatctctat gattcttatg atattcattt ttgttaaatt gtttatctta ttcgaacaca
      181 cgaaacaaac gttgcatgat ccaatcatcg cctccgtttc accaactttt acgatgtcac
      241 tcatggttat ttgtacttat tttgtttcat atgaaagtat tgcaccaatt gtcaaattca
      301 tttggctgtg cgctgttatc ttccaagtga tccttgtctt ttatttcaat tatcaccatg
      361 ttatcaaagc tgatctttca attgaagcaa tttatccaag ctggtttatc atttacgttg
      421 gatttggggt catcactgta actgccggga acttcttccc acttattgga aaaattttct
      481 tttggatttc attgatctgc tatgcgttct tattgccgat catcatcaaa cgtgtctttt
      541 tcgtaagaaa tatggctcat ccgacacgtc cgctgattac gatcgttgca gcacctggtt
      601 ctctttgcct aacaggttat cttaaaaact ttgctgaacc taatttttat cttgtcgtag
      661 cattgttcgc tttatctcaa gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt
      721 tcaagcttcg tttttatcca agctatgcag cctttacatt cccgcttgtt atctctgcaa
      781 cagctatatt tactactgtt cattacttca acagtttagg tatctatagt agcgtattgg
      841 atttcttaaa agttgcggaa tgtctgtttg caagtgtagt tgtcttctac gttcttgctc
      901 attacgtacg tttcttaatt aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa
      961 tcgcttaata atcgattcaa taggttgtga aaaagctgtt ctgcatcaag taataagaac
     1021 ggccaaacaa aaacagctgt tcatgttttt gtactggttg ggcttattac cgcagatgca
     1081 agcttttgaa caccgtttac caagaggtcg taa
//
LOCUS       DBKOIJ010000307         1112 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000307 DBKOIJ010000000
VERSION     DBKOIJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.316"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(267..971)
                     /locus_tag="ACV5NJ_002916"
     CDS             complement(267..971)
                     /locus_tag="ACV5NJ_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486921.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          194 c          199 g          398 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aa
//
LOCUS       DBKOIJ010000308         1073 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000308 DBKOIJ010000000
VERSION     DBKOIJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.317"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>1073
                     /locus_tag="ACV5NJ_002917"
     rRNA            <1..>1073
                     /locus_tag="ACV5NJ_002917"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DBKOIJ010000309         1067 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000309 DBKOIJ010000000
VERSION     DBKOIJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.318"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..879
                     /locus_tag="ACV5NJ_002918"
     CDS             <1..879
                     /locus_tag="ACV5NJ_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486922.1"
                     /translation="AYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGV
                     LGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETS
                     QLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELL
                     QDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVI
                     EAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKI
                     DKQIDDFPEEIIDRIE"
     gene            complement(933..>1067)
                     /locus_tag="ACV5NJ_002919"
     CDS             complement(933..>1067)
                     /locus_tag="ACV5NJ_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HHY6486923.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          371 a          159 c          188 g          349 t
ORIGIN      
        1 gcttatgaaa aaaagaaatt attccacttt ttagcaatca acctgttggc tgaacaagct
       61 gattctttag atattgctag caatcagtcg ctattttttt cggatatagg attttaccac
      121 ttaaaaggag tattaggaga aaaatccgtt atttttatca ataagacact ctcttggctt
      181 tatcttcatg ataaattgga tcaaaaatac ttgagaaaaa atgaagatga aacaatcgaa
      241 tggttgaatg aacgattcat tgaaagtttt gaaaatcagt ttaatctgtt gcgagaagag
      301 acgagccaat tgatccgaag agagttaacc aaaatacatt ttaaactttt gcattacact
      361 attaataaag ctgatgaatt tccacttgat gtttcttttt ttagagaaaa ctatccagca
      421 ttttattgct atttgataga gtatataaat gatctaacgt caaaacgcaa agagttgctt
      481 caagataaat tttttctttt tttcagttat ttattattat tgatcaatca catacccatt
      541 caactgattt ctgaaccagt aaagatcatc atcgattttt cttatggtgc tgcctataat
      601 caatttatca agaaaaattt aagcttgtac attaatctga acacggaagt gatagaggca
      661 ggaaagcctg agttcccgga tgttataatc acgaatagga ataatctata tgaagaaagt
      721 gcatctcaag tagtggtttg gctcgatccg ccaagagcag tagactgggg gaatcttaca
      781 aagattttgt tgactatcca agaagagaaa tacaaagaca gcaaaattga caagcaaata
      841 gacgattttc ctgaagaaat catcgacagg atcgaatagt gaacttcttt tagaaagtcc
      901 gactgctttt tctaaaaata ttatttgtca aattaagcga gacaaaactt tccatttacg
      961 taactcaaaa aaacttctaa tggtgtttgg taatttagtg attttctagg gatatggttt
     1021 cttttggacg caacggatga gatgaatcct tgattgactt ggttgaa
//
LOCUS       DBKOIJ010000310         1067 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000310 DBKOIJ010000000
VERSION     DBKOIJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.319"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACV5NJ_002920"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACV5NJ_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HHY6486924.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DBKOIJ010000311         1042 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000311 DBKOIJ010000000
VERSION     DBKOIJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.320"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            11..958
                     /locus_tag="ACV5NJ_002921"
     CDS             11..958
                     /locus_tag="ACV5NJ_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HHY6486925.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          334 a          162 c          226 g          320 t
ORIGIN      
        1 aggtaaaagg atgagctgtt atcttatcac cggtggggct ggcttcattg gttctacctt
       61 agccaatcat ttaggagaag aacatgtggt aatcgtcgtc gatgacttat caatgggaaa
      121 agtagaaaat cttgatatga cgaaaaatat cacttttatt gagggagatg tcgctgagaa
      181 atccttgatg gaagagatca tgaaagctaa taaatttgat tatatctttc atttagcagc
      241 agttgctagt gtggcagatt cagttgcccg accagtagaa acacatcgtg tgaattttga
      301 aagtgtatta atgattttag aattgatccg caagtatcaa cctgatttaa aacggatcgt
      361 gtttagttct tcagcggctg tttatgggga tgaaccgact ttaccaaaaa aagaagagtc
      421 cgtgattcgc ccattgacgc cttatgcggt tgataaattc gctgcagaac aatatgtgct
      481 agattattgt catttatatg atgtacccgg tagcgcagtt cgttttttca atgtctatgg
      541 tccaaatcaa aatccgaatt ctccatacag tggtgtgatt tctattctcg tagatcgcta
      601 taagaaacaa atcgtaggag aagcaacttc atttacatta tttggtgatg gaagccagtc
      661 tagagacttt gtttatatag atgatgtaat ccaagcatta cttttagttg cgaatgagga
      721 agctgcactg gggaaacaat tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt
      781 gattcatgca atcaatgaaa ttctcggtgt agaattgaca cttgaataca aagaagagcg
      841 ttcgggtgat atccatgact ctctagcaga tattactaag atccagtcaa ttggctatca
      901 accgaaatat ggtattttaa gtggaatgac taattatttg aaaactgaaa tcaattaaaa
      961 catactaaga taagattaga gggtaaattc aggctctttg tcaataagga ctgatgagtt
     1021 ttgtaaaatc aaatctggga ta
//
LOCUS       DBKOIJ010000312         1026 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000312 DBKOIJ010000000
VERSION     DBKOIJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.321"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            149..841
                     /locus_tag="ACV5NJ_002922"
     CDS             149..841
                     /locus_tag="ACV5NJ_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HHY6486926.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          451 a          147 c          170 g          258 t
ORIGIN      
        1 attttttaaa tagataaaaa aggaggatta aatgagtatt tgatagtatt tatattaaaa
       61 ttacatatgt aatttaaaaa cgcttgacat tgtgtttgag atcacttact tttagcatgt
      121 aaaagtattg gattaaggag ctatgcaaat gaaaaagaaa tttattactg gtgggattgt
      181 cgttgcattt ctatgtctaa ttggctacac aggcacgcgg tacgcaaatg aaattatttt
      241 gtggggtgga gagacagata ttgatacaat caatgagaat ctgacacagc ttgatcatac
      301 attgacagat aaagagcaag cgattacaga tcttaccgct cgactagcac aaaaagaaca
      361 gacattgcaa gattcaaagg cagatgtcca agaataccaa acaaaagtaa atgaattaga
      421 aaaccaaaaa aatcaatcgg cagcagaaaa acaaaactta gaaagtcaat tagtcagcaa
      481 ggatgcacaa ctacaaagca aacaaaatga aatcaatgtc aaactagatg agatcaacca
      541 aaaaaatgat gaaatcaatc atctgaatgc agattggaca gcaagactac aagaagcaca
      601 agcaaaactt tcagaattac aaaatactgt aaatggtttg aatcaacaaa tcgcaacact
      661 gacacaagaa aaaacaaatg tagaaactca gttaacagat acacaaaaga aattagacga
      721 agcaactgct gaaaactcaa gcttgaaaca atatattcaa aagttagaaa aagcaaaaga
      781 tgaagtggaa gatacagctg ataaatctca gcaaattgtt gaagaacatt tgcgtaaata
      841 atctttctta acaagtaaac ctaaattagt aaagaagcaa cagtaatcta tactaagtag
      901 ttcagttttg gagtacaaaa tgacaaaatg tacttcaaaa tggactactt ttttcatttt
      961 aacttacata ccaaattaca tacaaaatga tttataaaat gcaattgaaa atgataattg
     1021 aatgaa
//
LOCUS       DBKOIJ010000313         1003 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000313 DBKOIJ010000000
VERSION     DBKOIJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.322"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            370..1003
                     /locus_tag="ACV5NJ_002923"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACV5NJ_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DBKOIJ010000314          974 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000314 DBKOIJ010000000
VERSION     DBKOIJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.324"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(114..458)
                     /locus_tag="ACV5NJ_002924"
     CDS             complement(114..458)
                     /locus_tag="ACV5NJ_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HHY6486927.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(451..885)
                     /locus_tag="ACV5NJ_002925"
     CDS             complement(451..885)
                     /locus_tag="ACV5NJ_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HHY6486928.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
BASE COUNT          291 a          176 c          163 g          344 t
ORIGIN      
        1 taaaaacgtt gtcgtggtag tttcaaacaa aattcaaaat ttcatcacat tcctaggaaa
       61 tacgcaaact tttctcactt atatagttaa atctgttgag aaaataatca ttattatatt
      121 tgttcaggat actctggcag atcagctttt agtttttcta cttgttcatt gatttgtgtg
      181 atacgtggtt cggcttggaa tttaaaatct tcgatatctt ttttgattgc ttcttggaaa
      241 gcaggaatca cagattcagc tgtttctttt gtttcgatca atgcgctttt aaaatgattc
      301 aggctgttat ttaaatcatt tgtcagagtt gttgcttctt ccaattcttc gatcagtttc
      361 tttcttgttt cattcccgct cctgggcgcc aataacagac ctccgacagt gccagcagca
      421 gctccaaata acagtccttt gaaaaagttt ttaatcatcg caggacacct gctttacgat
      481 tgtttcttga atagctttta gttcttccga tccataacta tcttgatgat tgacaaagtg
      541 aatagaaaaa tcatcctctt tgctgtagcg agggactaaa tggatatgtg aatgaaaaac
      601 agattgatag gctagttcac ggttgttatt caagatatta agacctttca tttctggaaa
      661 agctttttcc aatgcttgag caatcttagg cacacgcgaa aaaacctctc ctgcaagttc
      721 gggatcatat tcaaaaatat ctgctacatg acgtttaggc acaactaatg tgtgtccttt
      781 cgttgcttga gagatgtcta aaaaagcata gaccttgtcg tcttcatata ttttatagct
      841 tgggatttct tgattgatga ttttgcaaaa aatacagttt tccatataaa acatcctttc
      901 tataaagaat aaatacttca gcttaacttt accataagtt aaagctattg taaggaaaaa
      961 aacaaaatac cgtc
//
LOCUS       DBKOIJ010000315          974 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000315 DBKOIJ010000000
VERSION     DBKOIJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.325"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            239..829
                     /locus_tag="ACV5NJ_002926"
     CDS             239..829
                     /locus_tag="ACV5NJ_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HHY6486929.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT          355 a          111 c          200 g          308 t
ORIGIN      
        1 tgtaaaaatt cgttaggttt aaataactgg aaatatcgga aaatgggcat tatacatcac
       61 ggacatccct tcttttggaa aaaatgttac gatttttata agtaatattg tatccggtgg
      121 aatcaacgat gaagataaca tatccatcgt tgtagattca gtagcattga atgatgctct
      181 tgttgcaaga aatccgaata aaattgttaa gtataaattg aattaatagg agatatgaat
      241 ggaaattagc agtaaagaat atttaaaagc ggttcttcgt atggaaaaga atctatatat
      301 agaatctaaa ggacaacaca caaaattact aaaaataatg aaatcacatc cggactacaa
      361 cgcttggcgg tatactcgtc gaatgagaat tacatcttat tattattcaa agagaaagaa
      421 aaatatcatt tatgcgatta tgtatattgt gtcttgcaga agaatgaata gtttgggaag
      481 aaaatgtggt atagagtgtg gtgaaaatgt atttgatgaa ggtgtaagat tttaccatac
      541 acaaggaatt gttattaatg gtaacgccat aataggaaaa aattgttact tatatgggaa
      601 taactgtatt ggaaatgatg gaatcgatcc taagtgtcct gtaattggaa ataatgtacg
      661 tgtatgcgtg ggtgcaaaga ttattggtaa tgtaaaaata gctaacaatg tagttgttgc
      721 ggcaggagct attgttatca aagattgtct agagaatggt gcaattcttg caggtgtacc
      781 ggcaaaaata atagggtatg ttaatttaac aaaagtattt ttagaataat ttttagcgaa
      841 agaaggtgaa taacgatgcg tatttcaata aaaagatggt caggagcaga ttggacattg
      901 ttcacgttgg cattttttgc aatctccata gttcttgtat atacttttaa cgccccaaga
      961 gcaattttat attt
//
LOCUS       DBKOIJ010000316          956 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000316 DBKOIJ010000000
VERSION     DBKOIJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.326"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            4..>956
                     /gene="bsh"
                     /locus_tag="ACV5NJ_002927"
     CDS             4..>956
                     /gene="bsh"
                     /locus_tag="ACV5NJ_002927"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HHY6486930.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEK"
BASE COUNT          332 a          127 c          172 g          325 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaaca
//
LOCUS       DBKOIJ010000317          953 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000317 DBKOIJ010000000
VERSION     DBKOIJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.327"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..929
                     /locus_tag="ACV5NJ_002928"
     CDS             <1..929
                     /locus_tag="ACV5NJ_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HHY6486931.1"
                     /translation="KIMTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMN
                     GIAQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMT
                     MLLPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNY
                     MLILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVT
                     EELAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPT
                     HSVFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT          304 a          161 c          195 g          293 t
ORIGIN      
        1 gtaaaattat gactgcgtat cataaaaatg aaagattaaa gaaaatagtg gttattttga
       61 taaccggatt gaccgctgcg gtcgctttaa attatttttt gattccagcc aatgtctttt
      121 ctgcagggat gaatgggatc gctcaaatca tttcctcgct tttatatgaa tggtttcata
      181 tcaagataga cacaggaaca tttatttttc ttttgaatat ccctgtgttt gtattagggt
      241 ttctcaaagt aggaaaacga gcaacgatcc ttagttttgt gaatgttgta tgtgtatcgg
      301 tgatgacgat gctgcttcca gtcggacaga tcactgataa tattttgatg aatgccctag
      361 taggcggtgt tttattaggt cttggcgtag gattatcttt aaaaatgggc tttacaactg
      421 gtgggatgga tatcatctct cttgtacttt cacaaacaac tggaaaaaca gtcggaaact
      481 acatgctgat tctaaatggt atcattgtag tcatcgcagg cttcttcttt aactgggaaa
      541 gtgccttgta tacgattatt tccatttatg caatgactca agtaattgat gccatccaca
      601 cgagtcatca aaaagtaaca gcaatgatcg ttaccgtcaa gccagaagag gtaacagaag
      661 aactagcaaa acggatggta cgtggaatga cattactgcc gtctgtcggt ggctattcaa
      721 aaaaacaagg gaacatgatc atgatggtca tcacccgcta cgaaatgtat gatcttgatc
      781 agatcgttca tgaaatcgac gacgatgcat ttatcaatat tttaccaact cattcagtat
      841 ttggccggtt tgcaaatgaa aacgaacaaa aaatgtatcg aacaactggc gtattgccag
      901 aaataaaaaa tcgttcacaa aaaaaatgag gaatcaggtt tattttagaa taa
//
LOCUS       DBKOIJ010000318          949 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000318 DBKOIJ010000000
VERSION     DBKOIJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.328"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(53..124)
                     /locus_tag="ACV5NJ_002929"
     tRNA            complement(53..124)
                     /locus_tag="ACV5NJ_002929"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Gln,seq:ttg)
     gene            complement(136..208)
                     /locus_tag="ACV5NJ_002930"
     tRNA            complement(136..208)
                     /locus_tag="ACV5NJ_002930"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:His,seq:gtg)
     gene            complement(211..284)
                     /locus_tag="ACV5NJ_002931"
     tRNA            complement(211..284)
                     /locus_tag="ACV5NJ_002931"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Trp,seq:cca)
     gene            complement(291..371)
                     /locus_tag="ACV5NJ_002932"
     tRNA            complement(291..371)
                     /locus_tag="ACV5NJ_002932"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(335..337),aa:Tyr,seq:gta)
     gene            complement(373..448)
                     /locus_tag="ACV5NJ_002933"
     tRNA            complement(373..448)
                     /locus_tag="ACV5NJ_002933"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(413..415),aa:Phe,seq:gaa)
     gene            complement(484..556)
                     /locus_tag="ACV5NJ_002934"
     tRNA            complement(484..556)
                     /locus_tag="ACV5NJ_002934"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(521..523),aa:Asp,seq:gtc)
     gene            complement(566..638)
                     /locus_tag="ACV5NJ_002935"
     tRNA            complement(566..638)
                     /locus_tag="ACV5NJ_002935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:Val,seq:tac)
     gene            complement(654..725)
                     /locus_tag="ACV5NJ_002936"
     tRNA            complement(654..725)
                     /locus_tag="ACV5NJ_002936"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Glu,seq:ttc)
     gene            complement(740..829)
                     /locus_tag="ACV5NJ_002937"
     tRNA            complement(740..829)
                     /locus_tag="ACV5NJ_002937"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(793..795),aa:Ser,seq:gga)
     gene            complement(869..942)
                     /locus_tag="ACV5NJ_002938"
     tRNA            complement(869..942)
                     /locus_tag="ACV5NJ_002938"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(906..908),aa:Asn,seq:gtt)
BASE COUNT          266 a          260 c          218 g          205 t
ORIGIN      
        1 gagtccagcg cgtctgccaa ttccgccacg ccggcaaatt taaaaataat aactggggta
       61 gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac
      121 cccaagaata aaaagggcga atgatgggaa tcgaacccac gaatgccgga gccacaatcc
      181 ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc agcaggaatt gaacccacac
      241 taacggtttt ggagaccgct gttctacctt taaactatgc ccctataaaa tggaggagag
      301 tggattcgaa ccactgaacc ctaaggaacg gatttacagt ccgtcgcgtt tagccacttc
      361 gctactcctc catggtggcg cgggacagaa tcgaactgcc gacacacgga gcttcaatcc
      421 gttgctctac caactgagct accgcgccat attttaaaat tattaattga ataacaagaa
      481 cgacggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc
      541 cctacaccac ggaaccagtt cattatggag gttaacggga tcgaaccgct gaccctctgc
      601 ttgtaaggca gatgctctcc cagctgagct aaacctccaa cattcaatta aaatgacccg
      661 tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg cttgaccaac
      721 gggccaggta aatttgctac ggagagtaag ggattcgaac ccttgagaca gtttggcacc
      781 atctacatga tttccaatca tgctccttcg gcctctcgga caactctcca gagtgaaaga
      841 agctcaatac tctttctcaa aatgagaact ccggcagtag gactcgaacc tacgacatca
      901 tgattaacag tcatgcgcta ctaccaactg agctatgccg gaataacat
//
LOCUS       DBKOIJ010000319          942 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000319 DBKOIJ010000000
VERSION     DBKOIJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.329"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..224)
                     /locus_tag="ACV5NJ_002939"
     CDS             complement(<1..224)
                     /locus_tag="ACV5NJ_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HHY6486932.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGL"
     gene            complement(226..>942)
                     /locus_tag="ACV5NJ_002940"
     CDS             complement(226..>942)
                     /locus_tag="ACV5NJ_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HHY6486933.1"
                     /translation="LEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLLK
                     AMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKVF
                     QRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEPF
                     VGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVESV
                     FNEENLKKAYGDTIFIGEGE"
BASE COUNT          327 a          189 c          102 g          324 t
ORIGIN      
        1 agaccaaata caatcgcacc aataaaaaaa ttaataccta aaatataaga aattgctact
       61 cctggcaaaa cagcatgaga aatagcatca cccataagag acattcctct taaaataata
      121 aaacaaccta ctgttccaga aacaatccct ataactacag atgtaattag agcattctgt
      181 aaaaagtgaa agtcatacag accattaata aaattttgaa gcatattatt ccccctctcc
      241 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
      301 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
      361 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
      421 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
      481 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
      541 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
      601 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
      661 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
      721 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
      781 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
      841 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
      901 taaaaaatcg ttataacaaa cattaagctt tttcacttct aa
//
LOCUS       DBKOIJ010000320          924 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000320 DBKOIJ010000000
VERSION     DBKOIJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.330"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(61..291)
                     /locus_tag="ACV5NJ_002941"
     CDS             complement(61..291)
                     /locus_tag="ACV5NJ_002941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HHY6486934.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(385..723)
                     /locus_tag="ACV5NJ_002942"
     CDS             complement(385..723)
                     /locus_tag="ACV5NJ_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486935.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          291 a          191 c          102 g          340 t
ORIGIN      
        1 aaagatatcg aaaaaacaat aacataatat gtgtcaaaat ttttcataat aagaactccc
       61 ttactgttgc atactaacat aatccaaaaa ctcttctact tcagctaaag acattccact
      121 ttctttagtc caaaaaacag ttttttctat cgatccttct ctgtaaactg taaatgtcgg
      181 agtcgagtta attgagtagc gactcttgaa ttctttccac tcattttcat cttctctaac
      241 ttttgctaca ttcacgtaat aaatattttc ttttcccttt tctaccagca tatcgaatag
      301 cttcctgctt ttgatgatcg atttcttgtg ctacatctag taaattttca tcaatttcta
      361 tatcataaag tatttcacta gtgtctacat ccacaatata tactttttat cagtagcaat
      421 ttttgagtaa tccgttaaaa tgatctcagt ctcgtcactt tcatcagtat aaatatacgt
      481 taacaatgaa ctcacctttt caatcggtgt tagctctaag cctactgtat tttttactat
      541 tgagtcatta tttaccacca taacttcgta ctgtatatac catcctattt ggtttccatc
      601 aatatcagta attggctcac tagctaatgg agtctccgga acaatatttt cctgatattt
      661 cttaaactct tctgtagcac gaaaaaggtt atcatgttcc tcacttgaaa ctggttgagt
      721 catagaaaat gttacttggc taaacagagg gaaaataaac actacaatca acaaagtata
      781 aaaaaaagcc cttctttttc attttttatc ttccaatcac aaattgaaaa acttattcct
      841 gcaactttga gtctagtatc acaatcgact gactacaact attcatttac attctacttt
      901 aagttcctcc ttaaccatta attt
//
LOCUS       DBKOIJ010000321          910 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000321 DBKOIJ010000000
VERSION     DBKOIJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.331"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..355
                     /locus_tag="ACV5NJ_002943"
     CDS             <1..355
                     /locus_tag="ACV5NJ_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486936.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..776
                     /locus_tag="ACV5NJ_002944"
     CDS             615..776
                     /locus_tag="ACV5NJ_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486937.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQISMYPMS"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DBKOIJ010000322          908 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000322 DBKOIJ010000000
VERSION     DBKOIJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.332"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(21..251)
                     /locus_tag="ACV5NJ_002945"
     CDS             complement(21..251)
                     /locus_tag="ACV5NJ_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486938.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(510..623)
                     /locus_tag="ACV5NJ_002946"
                     /pseudo
     CDS             complement(510..623)
                     /locus_tag="ACV5NJ_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          322 a          143 c          135 g          308 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aaaattgaaa catccatctg gaatttgtct
      181 ttttcgttgc tttgctcata aaaatctgca tattcatcac taatattttt tctaataaat
      241 tcttccatca taaagatcac ctcgaaaaga gtatacgaac aaacgttctt tttgtaaagg
      301 tggaatttat tatactaaat aaataggtga acaagtactt gtgccaaatt atgtgccaaa
      361 aaaaatcgaa tttaataaaa cacaaacaaa aagaaatcct attatgataa catttcttat
      421 aataacaaac acaacagaag acgtgtaata gttagtcagg aacgtacaaa taacccctgt
      481 atcctttgcg gtacaggggg ttattttgta taagtagaca ttgtagtttc taaactgcta
      541 tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa aatagatgca
      601 tgcgtatgtc ttagtccatc aataaacagt cgaagagaaa atagggaaaa attgattact
      661 aaagaaaaac ttactaggtc gacggtgtag ttgattttca tacttcaata gattcgaatt
      721 aaacgatttc gattggctta taccagtcct atccaacgaa agatatatcg ttcttaactc
      781 tagcggtata acttgaagct ttacgtaaag ccaatgggaa tgaactggag atgacttttt
      841 attattttta gtaatagctg atgggttaat acttacaact ttccttttca aagtaccata
      901 taaaaaaa
//
LOCUS       DBKOIJ010000323          900 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000323 DBKOIJ010000000
VERSION     DBKOIJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.333"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>900
                     /locus_tag="ACV5NJ_002947"
     CDS             <1..>900
                     /locus_tag="ACV5NJ_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HHY6486939.1"
                     /translation="STGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKND
                     KQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIAR
                     ETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKI
                     ESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKN
                     VIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLST
                     TEFYIQGIDCLNLENLINGNQTL"
BASE COUNT          328 a          102 c          168 g          302 t
ORIGIN      
        1 attcgacagg tctatgttta ttagatgaat tcttggcgga tatcagtcta tttataatgt
       61 ttattgatgt aattgaagaa acatcaagaa attttttgct gtctaagttc agaaaatttc
      121 gtatgaaaaa tgataaacaa aaattattgg caaatgagat ttggagtagt ttagaagtcg
      181 aagaaagtaa acaagaactt ttgttgaaat ggatactttt tcttgtaaag aagttctatg
      241 tgaaaatacc agaaacccat tgtctgatag ttatgcacgg taaaataaca gcttctgcga
      301 tcgcaaggga aacaaataag ttgctgaata catatgttta tgaggctttc gatatgccaa
      361 ttgaaggtga gactagtgac ttaattaacc taatcaacaa tttttgtaaa agtatagata
      421 cgacgaatgg actcattctg ctagttgata tgggatcatt ggaacaaatg tatgaaaaaa
      481 ttgaatccaa tgtaataggt gatttggtaa ttttaaacaa tgtttctaca gctttagcga
      541 ttgaatgtgg gattcaagtt tgccaaaaaa agccaattag tcatttttac cagatggatt
      601 ttgattcgtt tcaagttaaa gttcagtatt ataaaggatt atcacaaaag aaaaatgtaa
      661 tagtatcttg tctctctggt gaggggattt ctgaaaaagt taaggatatt ttaaaacggt
      721 atctcgataa tcaagtggag attttgattt ttgattttaa cgcattaaag aaaatagcaa
      781 aggaaaaaga tactatgatt tttaagaata caatttgtgt tctttcaacc actgaatttt
      841 acattcaagg aatcgattgt ttaaatttag aaaatctaat taatgggaat caaaccttag
//
LOCUS       DBKOIJ010000324          897 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000324 DBKOIJ010000000
VERSION     DBKOIJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.334"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            168..362
                     /locus_tag="ACV5NJ_002948"
     CDS             168..362
                     /locus_tag="ACV5NJ_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486940.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..>897
                     /locus_tag="ACV5NJ_002949"
     CDS             631..>897
                     /locus_tag="ACV5NJ_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486941.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIV"
BASE COUNT          372 a          110 c          141 g          274 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgta
//
LOCUS       DBKOIJ010000325          896 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000325 DBKOIJ010000000
VERSION     DBKOIJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.335"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..182
                     /locus_tag="ACV5NJ_002950"
     CDS             <1..182
                     /locus_tag="ACV5NJ_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HHY6486942.1"
                     /translation="DLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWN
                     DLYHANEVLKIAEEK"
     gene            183..>896
                     /locus_tag="ACV5NJ_002951"
     CDS             183..>896
                     /locus_tag="ACV5NJ_002951"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HHY6486943.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSS"
BASE COUNT          345 a           98 c          159 g          294 t
ORIGIN      
        1 ttgacttatt aaataataaa ataagttttg aagaaaacat tgttaaagaa atgcctgaat
       61 ctgctaatga tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca
      121 aagaaaactg gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat
      181 aaatggaaat tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa
      241 atttgaaata tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct
      301 atcaacaaaa acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt
      361 tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat
      421 tagctacaga tactgtaggg aaaattgaag tgatacgtga ttcattctta caactagaaa
      481 gagaagataa tatatttgat ttaattatag atttagattt gacttcacct ttacgtgaag
      541 tttctaatct tgaagaatta atagatgaac atctaaaaaa tttagataaa gacgtctact
      601 tttcagttgt ttctgcaaga cgtaatccct attttaatat ggtaaaaaga attgaaaatg
      661 aagttactat agttaataaa tcaagataca catctaggca acagacacct gaagtttttg
      721 aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct aaaaaaaagt aattatattt
      781 ttgatgggca atgtgggata ataaaaatga gagattatct aattttagat attgatagtg
      841 aagaagattt taaatggtta gaatttttgt ttcctaaatt tttagaagat tcttcg
//
LOCUS       DBKOIJ010000326          895 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000326 DBKOIJ010000000
VERSION     DBKOIJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.336"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            252..875
                     /locus_tag="ACV5NJ_002952"
     CDS             252..875
                     /locus_tag="ACV5NJ_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6486944.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          330 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aagaa
//
LOCUS       DBKOIJ010000327          895 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000327 DBKOIJ010000000
VERSION     DBKOIJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.337"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..878
                     /locus_tag="ACV5NJ_002953"
     CDS             <1..878
                     /locus_tag="ACV5NJ_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HHY6486945.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          287 a          201 c          183 g          224 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcga
//
LOCUS       DBKOIJ010000328          818 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000328 DBKOIJ010000000
VERSION     DBKOIJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.338"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..216)
                     /locus_tag="ACV5NJ_002954"
     CDS             complement(<1..216)
                     /locus_tag="ACV5NJ_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486946.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKIT"
     gene            complement(223..771)
                     /locus_tag="ACV5NJ_002955"
     CDS             complement(223..771)
                     /locus_tag="ACV5NJ_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HHY6486947.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          273 a          172 c           83 g          290 t
ORIGIN      
        1 tgttattttt tcattgacta taactagttc atctggtaaa aaaattaacc aacaaacatc
       61 gacaaacagt aaatgatata atccatttcc acctccaagc atattcaata ctggtgaaca
      121 tgccttagaa acacgaatat agttatggtt taaatagata ttttctttaa acattgcttg
      181 tttaaaagcc atatgctctt gttctaaact tttcataaac ttttactcta atgcacagct
      241 agcagcagct gctgcaattc ctgctacaat atttacctta gctaactctt tagctgcagc
      301 ttttcctaat tgtttttctg caatttttaa taataaatcc gcagcttttt caaaattaaa
      361 agcttttaat gcttcaataa aatctccctg cgtacctata gcccaagcca ctcctcctcc
      421 aggaagagaa ttaatggcta cacatccagc atatcgtttc caatcaactt tctccattct
      481 aggaacaata atatgttcat aaaagactct tgctgctctt ccttgttcgt tatcttgact
      541 gattcgttca cgaatagcac caaaatcctt tatatggtat acaccatgtt cgtctaaaaa
      601 accaactgta tcaaaataaa aagttaattc atctactaat ttattcttta tttctaaatc
      661 aactgtattc actttttctg tggcatacac acttgttcca gtactaacaa aagtacttaa
      721 acctaataag aaaacaccta aaacaccaaa tattttaatt attcttttca taatttctcc
      781 tccataaaaa ataagttact ttttaaatat cttgttta
//
LOCUS       DBKOIJ010000329          815 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000329 DBKOIJ010000000
VERSION     DBKOIJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.339"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            135..308
                     /locus_tag="ACV5NJ_002956"
     CDS             135..308
                     /locus_tag="ACV5NJ_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486948.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          274 a          131 c          103 g          307 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaa
//
LOCUS       DBKOIJ010000330          808 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000330 DBKOIJ010000000
VERSION     DBKOIJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.341"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            81..719
                     /locus_tag="ACV5NJ_002957"
     CDS             81..719
                     /locus_tag="ACV5NJ_002957"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HHY6486949.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          255 a          133 c          127 g          293 t
ORIGIN      
        1 ccataaaaaa acattgccta ttttaactct tcatgtatac tgactctatc ttcaaaaact
       61 gaatagtagg agttattatc atgaataaca ttttaggaaa aaaaatagaa cttaaaaaag
      121 ggatattggg cactattgtc gttgtactcg gtttgttagg gttgctattt gcgccatcca
      181 ttattagcat tttttcttta actatcactg gatttatagc atttacactt gtttttggta
      241 aaaaagatac gaaaaaaatg ttctcgaaac ctgtcgctcc tgtcaaaaat atagccaaat
      301 atttttttat caatgtagta atcagtgccg cagtctcggt atttttgcaa caaattttga
      361 aatggggttt aacaggcaat ccaataaatg aggctttcag cccgttatta tttattattt
      421 tgcctatcat gatacttgga gaagaattgt tctcagtgta ttttctcgct attttttcta
      481 gtaagttcag cattcctgtc gctagtttct tgtcagcaat catctttgga tttatccatt
      541 acagtactta tgataatgga aatattttac acactgtagc acacatcctt ttgatacaag
      601 gggtggcaag attgttattc aaccaagctg ccattaaaag caactcgatc attacaagtt
      661 gggcagttca tgtgatattt gatttcacag ctatattgct agtcgtttta ttctcctaaa
      721 tttttataga aataatacat gctctattgc taagaaagca tactttattt cctttaaaca
      781 aaaagacaga tgtatcaatg gattaaaa
//
LOCUS       DBKOIJ010000331          803 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000331 DBKOIJ010000000
VERSION     DBKOIJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.342"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            366..>803
                     /locus_tag="ACV5NJ_002958"
     CDS             366..>803
                     /locus_tag="ACV5NJ_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HHY6486950.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEF"
BASE COUNT          273 a          149 c          128 g          253 t
ORIGIN      
        1 ataacaaaac cgaatgcaaa acagaagttc tataagttat aatcatagtt gttaggtgat
       61 tcatctagta aaaacaccag atttcaaatt tcacacaatt taaactagat caatcatcaa
      121 ccttcttcaa attgttagaa ccctttcccg aggagctgca gcataagcaa aaaaacaaac
      181 aggaagccaa atggctaaga cattttttct attagtctag ttgcttctcg tcgcaagttt
      241 aagactttag aaagttattt ctaatgatgt ttgggttgtt ttgatcaatg cttatgcaca
      301 gctccttacc tattatttat ctcaacagat ctaacaaatt aatggactat ttaaggagtt
      361 tttttatgct tacgatctat tcttctcgca cacatagtta ccatctgatg gcaaactggc
      421 tgaaagacca tcaaatgcct tacagagaac gaattttcac acctaaacag cctttaacaa
      481 ggaaagagat attttattta ttgtcattaa cagaaaacgg atttgacgat ctattagcta
      541 aacgctcgat tgattataaa aatttgaaag accaaatcga ccaatgtacg acctcagagc
      601 ttgtcgatct gatcgtctcg cattttactt tgttacaacg tcccatcatt actgacggaa
      661 agaaattatt agtcggattc aatgaagata gcatgcgtct ttttatccct gtacaatggc
      721 gcagaagtca actgaaatct gtctataacg attttacatg ggtaagcaat ctagaaaatt
      781 ctacggattt agatgatgaa ttt
//
LOCUS       DBKOIJ010000332          795 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000332 DBKOIJ010000000
VERSION     DBKOIJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.343"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..210
                     /locus_tag="ACV5NJ_002959"
     CDS             <1..210
                     /locus_tag="ACV5NJ_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486951.1"
                     /translation="SLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSIS
                     IIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(414..779)
                     /locus_tag="ACV5NJ_002960"
     CDS             complement(414..779)
                     /locus_tag="ACV5NJ_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486952.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          317 a          101 c          141 g          236 t
ORIGIN      
        1 agtctgtctt ctaaaaaaaa ttgggtatac tcacaaacta ttttttatgg aggaattgtt
       61 ttaattagtt tactttcttc aacactttat tctttgaata ttatagatgt ttccacctca
      121 aattcaatat ctattattgg aatcattatt gctgcaatta ttacacaact gtttttagtg
      181 tttggagaga agaaaagaag taaaaaatag aaaaactagt gatagttttg gtgatagtga
      241 tattaaaaat cagaaaagat agaaaaagca ggagatagat aaaatgctga tatttcagca
      301 ttttttaaga gtataaaaga gaaaaaaagt aataaatgta tacatgatat aagaaaaaca
      361 tatatgtata aaaagaagaa agtagcttct ctttgtacat atatggtttg tttttatgga
      421 tgccgagctt ttttataaga taaggctacc gcccataacg acccaaggat ttgtggaact
      481 aagtaaatac ctgcaagaag caagatcagc tgggtcggtt ttaaaggatg atagaagtaa
      541 cgccattcag taaaattggc gcaaaataaa taggaagtga cagtagatat aatattggca
      601 atgaataaag atataatacg cctgttcctt tgaaaataat atccagcaaa aagagataaa
      661 aatatcaatg tgatgaaccc gcttacggaa tgataacgaa aatcaagcag taaggcgaaa
      721 tgcacataag gaacaaaaga taaaagcaac ggaaggtttt tccagcaagt ttttatcatc
      781 tttcatcacc accta
//
LOCUS       DBKOIJ010000333          776 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000333 DBKOIJ010000000
VERSION     DBKOIJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.344"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            478..>776
                     /locus_tag="ACV5NJ_002961"
     CDS             478..>776
                     /locus_tag="ACV5NJ_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HHY6486953.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSV"
BASE COUNT          293 a          106 c          123 g          254 t
ORIGIN      
        1 aaaactttag caacctccaa aaaggcttta tatcaatctt aaatagattt tagggtggac
       61 ataaagcgat gcgaacatgg acaaaaagcg atgtgaatgt ggacaaaaag cgatgcaaac
      121 gtggacaaaa agcgatgtgg tattccgaat atatccaaaa aaagaaaaag tatgtgaatt
      181 tatttctaaa aactagaaaa atatttgttt tttaattttg gtaattttta taatttcgta
      241 ttttattgaa aaggattttt attttgaaaa tgttctaaaa tagcgatttc tgctgtattt
      301 tcaatgaaat ttgattggaa aaaataaaat gtgttttact gacattgagt tatgttataa
      361 atttacgata catttcttat tgtaacgtat cgaaaacagg tctatactta ttcaatggaa
      421 tcgtaccagt tccaagtttt taagctcgta ccaagcttag aaagaagagg caatcgtatg
      481 aaaataacag aaaagaatgt aaacccgaaa tttgaaacta ccgaaagcaa tctaaaaaag
      541 tttgatttaa ctgttacttt atcgaatcaa cttgtagata gcattgttga aatggatgct
      601 atgccacaaa aaatttatct ttacgctata ggacatcttg accctaaaaa tccgcctttt
      661 aataataccg tcaaagtatc taaaaaggct ttatttgcgt tgatgtcttc tagttcatct
      721 tcttcaaaac attcaagatt aaaacaagtt attgaaaaat tgcatcagca atctgt
//
LOCUS       DBKOIJ010000334          773 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000334 DBKOIJ010000000
VERSION     DBKOIJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.345"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACV5NJ_002962"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACV5NJ_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HHY6486954.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="ACV5NJ_002963"
     CDS             complement(481..675)
                     /locus_tag="ACV5NJ_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486955.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          229 a          162 c          134 g          248 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaag acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacgtt aaaaatgttt tgc
//
LOCUS       DBKOIJ010000335          749 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000335 DBKOIJ010000000
VERSION     DBKOIJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.346"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..613)
                     /locus_tag="ACV5NJ_002964"
     CDS             complement(<1..613)
                     /locus_tag="ACV5NJ_002964"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HHY6486956.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHI"
BASE COUNT          246 a          149 c           82 g          272 t
ORIGIN      
        1 aaatatggaa tatgatatct tcagatatat aatccctttc tgacggaaac ttgatattgt
       61 gctgttgaat tatctcagat gaatacagcg catgccaaac tgacattggc aatataaaat
      121 cgactttata actcggctca gtgccaatca ttccaagcaa cactttttca attatagcgt
      181 tatttgtaaa tatcgtacta tcatatcttg ctggctttga aataatatta ttatcatcat
      241 aatattcata atatccagca taacatgtgt ctgcattact cctttttata ccatttaaca
      301 ttgcttcaat ataattagct gcaacaaaat catcagaatc aataaaagca acatattttc
      361 cttttacatg ttcaagtcct gtatttcttg ccaatcccaa gccagcatta tttttatgaa
      421 taatacgaat ccttgaatcc tttaatgcat agtcttcaca tattttatta gatgaatcct
      481 tagatccatc atccacaatt ataatatcta ggttcttata tgtttgatta cacactgatt
      541 ccaaacaact tcctacatac ttttcaacat tatatacagg tatgattaca ctaattagat
      601 cactcgaatt catccttcta tcctttcact cacgttttct caattagtca atgacggcat
      661 caacgatttt cttaaattct ctgaaaaaaa tagactctga tcttgatgat gtgaatgaca
      721 tactatcttc ttctagaaaa attatttcc
//
LOCUS       DBKOIJ010000336          749 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000336 DBKOIJ010000000
VERSION     DBKOIJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.347"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(17..>749)
                     /locus_tag="ACV5NJ_002965"
     CDS             complement(17..>749)
                     /locus_tag="ACV5NJ_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HHY6486957.1"
                     /translation="ICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          285 a          132 c           88 g          244 t
ORIGIN      
        1 gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat
       61 aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca
      121 tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca
      181 ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga
      241 tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt
      301 ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa
      361 attttgcatc gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc
      421 ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat
      481 cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg
      541 actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag
      601 cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt
      661 tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca
      721 aaagaaataa tactaaaaaa gaacaaatt
//
LOCUS       DBKOIJ010000337          749 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000337 DBKOIJ010000000
VERSION     DBKOIJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.348"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            174..410
                     /locus_tag="ACV5NJ_002966"
     CDS             174..410
                     /locus_tag="ACV5NJ_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HHY6486958.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            429..644
                     /locus_tag="ACV5NJ_002967"
     CDS             429..644
                     /locus_tag="ACV5NJ_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HHY6486959.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          244 a          103 c          180 g          222 t
ORIGIN      
        1 tccacaaaaa ctttgaaaac agttcttgaa cactaacttt ttcattcctg aatcaaattt
       61 attttggaaa ttttactatt caagaataaa cttgataagt ttcaggaaga tttttactgt
      121 taatcaaata cgtatgttac agtagcaccg taaaaaatat tatttaaaga agcgtgttta
      181 aacatgcaaa atgtaaagga actaagtatt gtggaaatgc acaaactgtt ggtggaggca
      241 ataatgcttg gggaaaattg gggcaagttg taggtggact taccacaggg gcagtaggtg
      301 gtgcaggttt aggaacagca atttgcggac cagcttgcgg tgttgttgga gggttatatg
      361 gagctgtcgc tggaggtgct gcagctggct gggatgctcg aaaaaaataa aaaaggagaa
      421 taagtagtat gcatattaaa aacacaaaaa caacatttat attatcttca gaagagttga
      481 aaaatattca aggtgggagt gccgtgggag ttttaggcac tactttttct ggggcaacag
      541 caggagtaaa actttgttca gccggaggac cttatgcaat agctgcttgt ggtgtaggtg
      601 gagctttatt aggtgctgga ttcagcatgt ggaccggtgc gtaaacaatt aaaaaatatc
      661 aaacctcaag attggtttgt gtcgattacg aacataggta tgttctttat cttttcaaaa
      721 gtttggaatg gacagaactt tttactaaa
//
LOCUS       DBKOIJ010000338          737 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000338 DBKOIJ010000000
VERSION     DBKOIJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.349"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            1..737
                     /locus_tag="ACV5NJ_002968"
                     /pseudo
     CDS             1..737
                     /locus_tag="ACV5NJ_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          249 a          140 c          140 g          208 t
ORIGIN      
        1 cttagaggag accgtcattc acctcttccg aaaaggtatt accatgtcgg aaatcgcaga
       61 cttgattgag aaaatgtatg ggcatcacta cacgccccaa accatgtcca acatgactaa
      121 agtgctgact gaagaagtaa atgcatttaa agttagaact ctaaatgata agtatgtcgc
      181 tattttcatg gatgctactt atattccttt aaaacggcaa accgtctcca aagaagcaat
      241 ctatattgcc attggtatac gagaagacgg cactaaagaa gtactaagtt atgcgattgc
      301 tccgactgaa tcgacctacg tttggaatga gttgttacag gacatttact ccagaggggt
      361 tcatgatgtc ttactgttta ttaccgatgg cttaaaaggc atgaaagata cgattcacca
      421 gatttatcct aaagctaaat accaacattg ctgtgttcac gtttcccgta atattgctca
      481 taaagtacgt gtcaaagatc gaaaagaaat ctgtgatgat tttaaggctg tttatcaagc
      541 tagctcaaag gaagaggcaa ataccttttt agggagtatg attgagaagt agcaaaaaac
      601 ttatcctaaa gtgacgcagt cactgataaa aaatcaagat ttattgactt tttatgagtt
      661 tccgcctggt atccgccgaa gcatttactc aactaattta atcgaatctt tcaagaaaca
      721 aatcaagaaa tacagcc
//
LOCUS       DBKOIJ010000339          729 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000339 DBKOIJ010000000
VERSION     DBKOIJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.350"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            18..>729
                     /locus_tag="ACV5NJ_002969"
     CDS             18..>729
                     /locus_tag="ACV5NJ_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HHY6486960.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQ"
BASE COUNT          272 a           97 c          142 g          218 t
ORIGIN      
        1 cataggagga gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt
       61 ccattggaag tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt
      121 atagtagagg tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt
      181 tgagattttt aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg
      241 tggatattat ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta
      301 tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa
      361 ataatgtaaa acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca
      421 tgggtgcgac aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa
      481 aaacagaaac aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg
      541 ttattcctct ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg
      601 atatgacacg atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta
      661 tgaattcaaa atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt
      721 tagctcaat
//
LOCUS       DBKOIJ010000340          723 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000340 DBKOIJ010000000
VERSION     DBKOIJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.351"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(428..652)
                     /locus_tag="ACV5NJ_002970"
     CDS             complement(428..652)
                     /locus_tag="ACV5NJ_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486961.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          266 a          105 c          130 g          222 t
ORIGIN      
        1 aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta ttcgtttatt
       61 gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc
      121 tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct acttgagatt
      181 ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc
      241 gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac
      301 agtacctctt tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt
      361 gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct
      421 gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata
      481 aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca
      541 tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc
      601 gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt
      661 catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta
      721 cgt
//
LOCUS       DBKOIJ010000341          717 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000341 DBKOIJ010000000
VERSION     DBKOIJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.352"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..608
                     /locus_tag="ACV5NJ_002971"
     CDS             <1..608
                     /locus_tag="ACV5NJ_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HHY6486962.1"
                     /translation="IGTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGT
                     GVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRL
                     GKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFA
                     KLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          201 a          142 c          182 g          192 t
ORIGIN      
        1 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
       61 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      121 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      181 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      241 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      301 aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      361 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      421 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      481 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      541 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      601 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      661 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DBKOIJ010000342          713 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000342 DBKOIJ010000000
VERSION     DBKOIJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.353"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..566
                     /locus_tag="ACV5NJ_002972"
     CDS             <1..566
                     /locus_tag="ACV5NJ_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HHY6486963.1"
                     /translation="FSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYVE
                     AAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKIARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPT
                     VISANAGPVTIPVPGGGFTWDNTITNW"
BASE COUNT          248 a          124 c          124 g          217 t
ORIGIN      
        1 atttttctaa aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag
       61 ataactttat tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgcg
      121 ctgcttacgt agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta
      181 gagccattat gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc
      241 ttaatcaaga tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaaatag
      301 cacgaatact aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata
      361 tgggaactgc tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt
      421 gggtcaatca aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa
      481 ctgtaataag tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt
      541 gggacaatac cattacaaat tggtaaataa actcattgct ttagttatgg gtctagtgtt
      601 agttcttttc ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc
      661 ttttctagga acttggcaaa ctaaagatca ttcagaacgt ctagtaatca cta
//
LOCUS       DBKOIJ010000343          708 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000343 DBKOIJ010000000
VERSION     DBKOIJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.354"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            220..>708
                     /locus_tag="ACV5NJ_002973"
     CDS             220..>708
                     /locus_tag="ACV5NJ_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HHY6486964.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          274 a          114 c          115 g          205 t
ORIGIN      
        1 tttacttatt aacaggctgt agaaatataa aaaagaacta aaatttaact aaattatgtg
       61 ctattcttaa ggaaagccaa gtgtatgaga aagtttctga gaatataatt ttgagagtat
      121 aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aagtcccttc tgtagaataa
      181 agttaacgac aaccaattca cagaaaagag gacttcccta tgaatgattt tactacagaa
      241 attgtgcaaa ctctagtcac taaaggcgat ttaaatgaat tattccgttc gcacttagaa
      301 aaagcgataa acacactcct acggactgaa ttaacggctt ttttagatta cgaaaaatat
      361 gatcgcactg gttttaattc aggtaattcg agaaacggtt cttactttcg atcaatcaaa
      421 accgaatatg gtgaattaac attggaaata cctagagatc gtaatggtga gtttaaacaa
      481 caaactttac cagcctacaa aagaacaaac gatacattgg aaaccactat tatccattta
      541 ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga tcgaaaaaat gtacggtcat
      601 cactatactc cacaaaccat gtccaacatg actaaagttc tgactgaaga agtaaatgcc
      661 tttaaatcca gagccttaaa tgataagtat gtcgctattt ttatggac
//
LOCUS       DBKOIJ010000344          693 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000344 DBKOIJ010000000
VERSION     DBKOIJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.355"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            132..344
                     /locus_tag="ACV5NJ_002974"
     CDS             132..344
                     /locus_tag="ACV5NJ_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486965.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>693
                     /locus_tag="ACV5NJ_002975"
     CDS             506..>693
                     /locus_tag="ACV5NJ_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HHY6486966.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          247 a          103 c          122 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct aga
//
LOCUS       DBKOIJ010000345          690 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000345 DBKOIJ010000000
VERSION     DBKOIJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.356"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..461)
                     /locus_tag="ACV5NJ_002976"
     CDS             complement(<1..461)
                     /locus_tag="ACV5NJ_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HHY6486967.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          219 a          126 c           98 g          247 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac
//
LOCUS       DBKOIJ010000346          687 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000346 DBKOIJ010000000
VERSION     DBKOIJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.357"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>687
                     /locus_tag="ACV5NJ_002977"
     CDS             <1..>687
                     /locus_tag="ACV5NJ_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HHY6486968.1"
                     /translation="HQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER
                     SVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFN
                     SEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKN
                     FFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMD
                     FRKVNQTFISS"
BASE COUNT          234 a          127 c          128 g          198 t
ORIGIN      
        1 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
       61 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
      121 cgaaaagaaa ggtctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
      181 atatttgata ttgacacact accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa
      241 aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa ttatcctgat
      301 ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca tcataaaagt
      361 gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa acccaacggc
      421 cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt ccctaaaaac
      481 ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt
      541 aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca acgcccatta
      601 aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat ggattttaga
      661 aaagtgaatc agacatttat ttccagt
//
LOCUS       DBKOIJ010000347          646 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000347 DBKOIJ010000000
VERSION     DBKOIJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.358"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            111..593
                     /locus_tag="ACV5NJ_002978"
     CDS             111..593
                     /locus_tag="ACV5NJ_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486969.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          183 a          101 c          122 g          240 t
ORIGIN      
        1 tgaataaact ctcatgccat tatctctaaa aaaaaagttt ttccccctaa tttttttaga
       61 tagaaaattg tatagtatga tgagagaaaa agtagtttgg gggaacgtta atgagaagaa
      121 aagcagtaat cgatcggctt tatttatttt tttggttttt tcctatcgtt ttgctacctt
      181 ttacatgggt aaatgctgtt gtacaaggtg cggagatatt aggcctgatc caaggaagcg
      241 gatttgtggt ttctaggaat tttccagctt tgactgtttt ctatttagtg ataagtgtag
      301 gtctcttatt accaaaatct ctgatcaatc actacaattg gcagctgctt tacttttctt
      361 gcttattagt atttacatct atgttttctt tgctgcctcg cttgattatc ggccctaatg
      421 gtttgagcga gagtggattg aaggattcag cttattcctc catgtatttc ttgacgatca
      481 aaccatggtt ctatttagta attgcctcat ttattcttgc aacatttttt tacttctgga
      541 cagaaatcag gataaaaagt gaacgatctg tatctataaa aaataactat tgatggtgga
      601 tcatcagtaa aaaagcatat gagctgcgta aaatgcatgt ttctca
//
LOCUS       DBKOIJ010000348          645 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000348 DBKOIJ010000000
VERSION     DBKOIJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.359"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(14..643)
                     /locus_tag="ACV5NJ_002979"
     CDS             complement(14..643)
                     /locus_tag="ACV5NJ_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HHY6486970.1"
                     /translation="MLGAGIYGLAVTGINIPSKLADGGVTGIALLLHNLFGFAPSVTS
                     LLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFAVDSLLINSLMTGI
                     LSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGILVVSLLYLDLTHF
                     LYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLEQLEH"
BASE COUNT          251 a          119 c          118 g          157 t
ORIGIN      
        1 tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg
       61 tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat
      121 tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca
      181 aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct
      241 taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc
      301 cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga
      361 tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa
      421 ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg
      481 agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa
      541 gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat
      601 tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatga
//
LOCUS       DBKOIJ010000349          635 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000349 DBKOIJ010000000
VERSION     DBKOIJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.360"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..447)
                     /locus_tag="ACV5NJ_002980"
     CDS             complement(<1..447)
                     /locus_tag="ACV5NJ_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486971.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          186 a          116 c           87 g          246 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttat
//
LOCUS       DBKOIJ010000350          593 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000350 DBKOIJ010000000
VERSION     DBKOIJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.361"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..580
                     /locus_tag="ACV5NJ_002981"
     CDS             <1..580
                     /locus_tag="ACV5NJ_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HHY6486972.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DBKOIJ010000351          583 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000351 DBKOIJ010000000
VERSION     DBKOIJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.362"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(38..>583)
                     /locus_tag="ACV5NJ_002982"
     CDS             complement(38..>583)
                     /locus_tag="ACV5NJ_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HHY6486973.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          100 g          206 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gat
//
LOCUS       DBKOIJ010000352          573 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000352 DBKOIJ010000000
VERSION     DBKOIJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.363"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            104..484
                     /gene="yajC"
                     /locus_tag="ACV5NJ_002983"
     CDS             104..484
                     /gene="yajC"
                     /locus_tag="ACV5NJ_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HHY6486974.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
BASE COUNT          218 a           86 c          127 g          142 t
ORIGIN      
        1 cttctaaagg aaaatctttt aaaaattaag taaaaaacta gtgttatcag catttttctg
       61 ctaaagtgtt ttttgtagat acagaattag tgaggtgaaa aaaatgggaa cattaccaat
      121 gcttgtgatg tttatcgcgc tattagcaat gtggtttttt atgtcacgtt cgcaaaagaa
      181 acaacagcaa gaacgtcaaa acttgttaga tagcatgaaa ccaggtgatt cagtcgtaac
      241 gatcggcgga ttacatggag ttgtttcaga aatcgacaat gacaaacgga cagttgtttt
      301 agactgcgaa gggatcttcc ttgagtacga tcgtgcagca atcaaaacgg tcaaaccagg
      361 gactgtcgta acaaatgatg caacgactac tgttgaaaca acagaagaac cagctgagac
      421 agttgaagta aaaactgaaa caacagaaac gctggaagag acagaagaga aggaagaaaa
      481 ataagtatca aagcttattt aaagacgagg tagggcagaa agatgtccta cctcattttt
      541 tattcaaaaa agaaagagat aagagcatgg gat
//
LOCUS       DBKOIJ010000353          571 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000353 DBKOIJ010000000
VERSION     DBKOIJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.364"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..435
                     /locus_tag="ACV5NJ_002984"
     CDS             <1..435
                     /locus_tag="ACV5NJ_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HHY6486975.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          183 a          130 c          110 g          148 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc a
//
LOCUS       DBKOIJ010000354          565 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000354 DBKOIJ010000000
VERSION     DBKOIJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.365"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            1..296
                     /locus_tag="ACV5NJ_002985"
                     /pseudo
     CDS             1..296
                     /locus_tag="ACV5NJ_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          193 a          100 c           71 g          201 t
ORIGIN      
        1 cttcataaga tgtacaatat gacattaaaa aaataaaagc ttacgaccat ccgttaaatc
       61 tgacactaaa taatggactg ctttcttttg tattagcagg ttacgttgac tatgaatatt
      121 caccagatta tttggagaaa cctcttcaac acgagaacaa tgatattaat tttagatttg
      181 tctacgagtc aaaaagtaaa acatggttag ttgatcaatt aagcacgata aacacaagct
      241 tccatgaatt acctaaacct gaaaatgaag ttgagatggt cattaaaatc ttctaaaaaa
      301 gaagcgttga aggggaaata ttcctttcaa cgctcttttt tatatgctta gaaacataaa
      361 taaattgtat actttctttt tagcccctat tacttttcac gaaaaatatc tttttttcga
      421 cgctagcgtt tctctacttc taatactgta cacagttttt gtattattga aaaattactc
      481 attatttagc attaatccac atcgttttat caatcgacct tcatactaaa ctactttaaa
      541 gtcatcttcc ttctcgtttt tcatt
//
LOCUS       DBKOIJ010000355          559 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000355 DBKOIJ010000000
VERSION     DBKOIJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.366"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            134..532
                     /gene="spxA"
                     /locus_tag="ACV5NJ_002986"
     CDS             134..532
                     /gene="spxA"
                     /locus_tag="ACV5NJ_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HHY6486976.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          197 a           88 c          110 g          164 t
ORIGIN      
        1 cattttttta aaaaaattat gaaaaaaagg tagctattta aaagttgatt atgtataata
       61 gaaacagatg ggagattatt atgatcttct gttacaaaaa agtgattctt atcacgaaaa
      121 ggagtggtgc acgttgttaa cattatatac ttccccaagt tgtacttcct gcagaaaggc
      181 tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa cgtaatattt tttcagaacc
      241 attgaacagt tctgaattaa aagcgatttt acaaatgaca gaagacggaa cagaagaaat
      301 catctcgaca cgttccaaag tattccaaaa actgaacatg gatttagatg atctgccgtt
      361 acaagaatta ttggaacttg tgcaaaacaa tcctggattg cttcgccgtc caatcatgat
      421 tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa attcgtcggt tcctaccgcg
      481 cgatgtacga caattagaat tacgtcaagc acaattgatg gctggattat agaaggataa
      541 tacgaaactt ggaaaagac
//
LOCUS       DBKOIJ010000356          540 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000356 DBKOIJ010000000
VERSION     DBKOIJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.367"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            96..440
                     /locus_tag="ACV5NJ_002987"
     CDS             96..440
                     /locus_tag="ACV5NJ_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HHY6486977.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          207 a           82 c          111 g          140 t
ORIGIN      
        1 taaatttaaa cgagaatctt acttctaaat tctcatttac aagcagctaa aaaaagagta
       61 tgataaataa aaatcgtgtt aaggagtgtc gtacgatgag cgaagcaaac caagaacggt
      121 ctggacaaga aatagaacta atcaaagaac gtattcttgc tgccttggaa atggtgattg
      181 atcctgaatt aggaatcgac atcgtcaatt taggcttgat ttatgatata gaattcaatc
      241 ctgaaaatgg agaaacagtc attaagatga ctctaacaac tatgggctgt ccattagcag
      301 atattttaac agaatctatc catgacgcat taaaagaagt gccagaagta acgaaagcag
      361 aagtcaaact agtctggtat ccagcatgga cgacagataa gatgagccgt tatgcacgga
      421 ttgcgttggg gatacgatag aaatcttggc tgactagcta tgagaagcaa ataggaaaaa
      481 caaaaaagcc ttttatcctt tctagaagaa aagagataaa aggtttcttt gtttataaaa
//
LOCUS       DBKOIJ010000357          536 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000357 DBKOIJ010000000
VERSION     DBKOIJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.368"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(62..135)
                     /locus_tag="ACV5NJ_002988"
     tRNA            complement(62..135)
                     /locus_tag="ACV5NJ_002988"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Ile,seq:cat)
     gene            complement(302..>536)
                     /locus_tag="ACV5NJ_002989"
     CDS             complement(302..>536)
                     /locus_tag="ACV5NJ_002989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486978.1"
                     /translation="LFEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVL
                     GISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT          184 a           87 c          100 g          165 t
ORIGIN      
        1 ttagcttgta taatactctt caaaaaattc taacacagaa cgattcaatt ggctacatta
       61 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      121 ctgagctaag ggtagtggtt gttgccacat aaagccataa acaatcaacc agtagaatgc
      181 gtggcaacaa acctgttatc gcatatcttg gagtgtgact atttatgagt gatagtgaag
      241 atatgcgtct ccatatatgt atggttgaga cagataaaca tgtatgaaaa acgttgttaa
      301 atcaacgttt ttttattttg gtcaaggaga gaaaagtaga gaaatacaaa attttttgca
      361 ttgattttgc attcaaaaaa acattcaaac atttagaaat ccccaatacg tattcaggaa
      421 aatctatttt aaggtttaca aaaagaactt ttgttcgtac actctttttg aggtgaactt
      481 ttatgctgat ggaaggaaaa acacaattat ggtttaagca tgttccttca aataag
//
LOCUS       DBKOIJ010000358          535 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000358 DBKOIJ010000000
VERSION     DBKOIJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.369"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>535
                     /locus_tag="ACV5NJ_002990"
     CDS             <1..>535
                     /locus_tag="ACV5NJ_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HHY6486979.1"
                     /translation="EKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWNYSGCNMPL
                     NLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFIIFVAIVFT
                     LKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLEYASRDSGY
                     DGVGDLNRITGISRVVD"
BASE COUNT          164 a           60 c           99 g          212 t
ORIGIN      
        1 ttgaaaaatt attctggatt tcactgccac tttgtttaat tgaaagattc atggtcacct
       61 atccaactgg aacaattgta ggagatatga taggtggagt tttttggaat tattcaggtt
      121 gtaatatgcc attgaattta attttgtgtg tgacagttac tgttgttaca atcggatatt
      181 tcaagggaga aaaaaatttc atccatttta ttttatgtgt ttgtgcagca ctttttatgt
      241 ctgcattagc tgaattgaaa gtttttattc ctgaattatt tataatattt gttgcaatag
      301 tattcacttt aaaagttgat tttaaagtag tgatcaagat attatcatgc gtaatacttg
      361 tgtcgatagt attatccttt tttataacaa tatttattac attgaacgat aatggacaaa
      421 gctatgctga taatttcaca attcaaggtc ttttagaata tgcgtcaaga gattctggat
      481 atgatggtgt tggtgattta aatcgaataa ctggtatctc tagagtagta gatga
//
LOCUS       DBKOIJ010000359          532 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000359 DBKOIJ010000000
VERSION     DBKOIJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.370"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(3..134)
                     /locus_tag="ACV5NJ_002991"
                     /pseudo
     CDS             complement(3..134)
                     /locus_tag="ACV5NJ_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
     gene            228..>532
                     /locus_tag="ACV5NJ_002992"
     CDS             228..>532
                     /locus_tag="ACV5NJ_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HHY6486980.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     "
BASE COUNT          195 a           70 c           93 g          174 t
ORIGIN      
        1 atttataaaa ttgtcttatc gcttggttcc acaaccaagt agcgataagg ctcatcccct
       61 tcagaatgag tggtaacata aggattttta cttaaaacag aatgaatttg ttttctttca
      121 aaagcgggca ttggttagtc aaagactagt tcatttatat ttatatctta atcttttatt
      181 ttaataaatt gtgatataat gaagtataac tttttaggga gtgtactatg aattttcaag
      241 ataatagacc attatatcta caagtagaag cttctatacg taacgatatt cgtgaaaaaa
      301 aatatcttcc aggagagcaa ttacctacag aagaagaatt atgtaaaatt tatggagtaa
      361 gtaagattac cattcgaaaa gcttttaaac ttttaacaga aagtggaaca gttgaacgat
      421 taagggggaa agggaccttt gtaactcaaa aaaaagaaag tttaaaatta gctggagttc
      481 atggatttag cgattctctt tctacccgag gacataaaat tagatatact gt
//
LOCUS       DBKOIJ010000360          526 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000360 DBKOIJ010000000
VERSION     DBKOIJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.371"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..354
                     /locus_tag="ACV5NJ_002993"
     CDS             <1..354
                     /locus_tag="ACV5NJ_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HHY6486981.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DBKOIJ010000361          526 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000361 DBKOIJ010000000
VERSION     DBKOIJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.372"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..265
                     /locus_tag="ACV5NJ_002994"
     CDS             <1..265
                     /locus_tag="ACV5NJ_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HHY6486982.1"
                     /translation="EKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDKMYIIPPHY
                     HATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            277..>526
                     /locus_tag="ACV5NJ_002995"
     CDS             277..>526
                     /locus_tag="ACV5NJ_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HHY6486983.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFI"
BASE COUNT          219 a           65 c           98 g          144 t
ORIGIN      
        1 tgaaaaatat agaggtgaaa taaaagttgt aggtatcagg ccaggagaaa aattatatga
       61 agagctaatg actacagacg aaattgaacg ctcatatgaa aatgataaaa tgtatatcat
      121 tccaccacac tatcatgcta ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac
      181 tgcatactct tcagatgcaa cagaattaat gagtggacag gaatttatta gaatcgcaaa
      241 agagaataat gtcgtgactt ggtaaggagt tttatgatga aatcagaagt atatgtaatt
      301 gcagaaattg gtgtaaacca taatggaagt ctagatttag ctttagaaag tattgataaa
      361 gctaaagaat gtggagctaa cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt
      421 tccaaaaagg ctccaatggc aaaatatcag aaggataatc ttaaagagga cagttctcaa
      481 tttgaaatgc taaaaaaatt agaattatct caatctgatt ttatta
//
LOCUS       DBKOIJ010000362          513 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000362 DBKOIJ010000000
VERSION     DBKOIJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.373"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..418
                     /locus_tag="ACV5NJ_002996"
     CDS             <1..418
                     /locus_tag="ACV5NJ_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HHY6486984.1"
                     /translation="YLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGI
                     ILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQ
                     GLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          193 a           63 c           91 g          166 t
ORIGIN      
        1 atatttgaaa agtttgttga tagcgaagct ataccaagca aataacaata agatttgttg
       61 taaattcgaa tgtccaagta ctattattga agttcctgtt ccaatatttg actgtattag
      121 ggtgcttgga attattctcg ataatgcaat tgaagcatct atagagagtc aagatagaga
      181 actgtctcta gctatttacc gagatgataa acagatagaa atttcgatat cgaatagttg
      241 tagagagata ctactctcta tagatactct aatgaaaaaa ggaataacaa caaaaaaggg
      301 acatcaaggg ttaggtttat ctagtataga ggaaatcaat aaaaagaatc ccaatatgtt
      361 tgtgaactat caaaacgatc cgtatcaatt tactactcaa gttattttgt tgttttgagg
      421 gaaggaggat ttatgagtta tccaataata ttatgtgaag atcaaattat acaattaaat
      481 cagttagaac gaattattga taatttcatt ctt
//
LOCUS       DBKOIJ010000363          512 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000363 DBKOIJ010000000
VERSION     DBKOIJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.374"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..343
                     /locus_tag="ACV5NJ_002997"
     CDS             <1..343
                     /locus_tag="ACV5NJ_002997"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6486985.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DBKOIJ010000364          509 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000364 DBKOIJ010000000
VERSION     DBKOIJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.375"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..406)
                     /locus_tag="ACV5NJ_002998"
     CDS             complement(<1..406)
                     /locus_tag="ACV5NJ_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486986.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNR"
BASE COUNT          167 a           94 c           79 g          169 t
ORIGIN      
        1 tccgattcag ctgatcatta agatcgaagt atacaaaatt attctgtagt ttataaaaaa
       61 acggcgaatg aagatgtgtt ttcatatgac caaaatataa taattctgaa atttctaaag
      121 gtgtcaattc tttcagcatc gttaaatcag taaagtcgat ccacttcaat tcatcgaatc
      181 cacgcctgtt acgagaagag gaaaagaaac gctgaatatt ttctgtacca cggattactt
      241 tcagccctgt atgggattca tattctccag cagaagcaga tggatcaagc aagagcaagt
      301 tttgaggaaa atgcacgata ctgtttgcaa agtcagtagc agtcagccct tttgttaaaa
      361 cagcattact tgtggtatca atatgtacat aaactgactc agtcatcatg ttcctcctct
      421 tatttcctct taatctcttt tattttacaa catttgcatt aaaaaagctt gtttctttta
      481 ttttaaaatt ccattacaaa atcacgttt
//
LOCUS       DBKOIJ010000365          502 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000365 DBKOIJ010000000
VERSION     DBKOIJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.376"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
BASE COUNT          168 a           96 c           58 g          180 t
ORIGIN      
        1 aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat
       61 tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact
      121 tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc
      181 aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt
      241 agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc
      301 aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa
      361 gacatctcga ttcatattca agataggaat ctttttatca acatcacttt attcgtattt
      421 tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact attcagccca
      481 ttcaacgacc aatgaatttt tt
//
LOCUS       DBKOIJ010000366          500 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000366 DBKOIJ010000000
VERSION     DBKOIJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.377"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..299
                     /locus_tag="ACV5NJ_002999"
     CDS             <1..299
                     /locus_tag="ACV5NJ_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486987.1"
                     /translation="AEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRL
                     QNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKKSCFLHCRFTKEI"
     gene            301..>500
                     /locus_tag="ACV5NJ_003000"
     CDS             301..>500
                     /locus_tag="ACV5NJ_003000"
                     /inference="COORDINATES: protein motif:HMM:NF044726.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HHY6486988.1"
                     /translation="MEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTN
                     IKFLLTRTMSVPVPYKQEQRDPA"
BASE COUNT          189 a           87 c           79 g          145 t
ORIGIN      
        1 gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg aatagcaaat
       61 acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc gatcttaaac
      121 ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc aaagatagag
      181 gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag gattacacaa
      241 taaaaaatac tgttaaaaaa tcctgttttc tccattgcag atttacgaaa gaaatttagt
      301 attgagaaaa aatataaagc ttttcgagac tttgaaagac gtgtaattaa agaaccgatt
      361 gcccaactga ataaatacac tgattttaaa attgactatg agcttatcaa gaagggtcat
      421 acttatacaa acataaagtt cttgcttaca cgtaccatgt cagttccggt accttataag
      481 caagaacaaa gagaccctgc
//
LOCUS       DBKOIJ010000367          496 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000367 DBKOIJ010000000
VERSION     DBKOIJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.378"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            88..>496
                     /locus_tag="ACV5NJ_003001"
     CDS             88..>496
                     /locus_tag="ACV5NJ_003001"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HHY6486989.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNY"
BASE COUNT          196 a           57 c           89 g          154 t
ORIGIN      
        1 tacttaaatt aggtataatt agtatatgtt ttgtgagaaa tttagactta caataagcag
       61 tgaatttata atcaacgtat aggagacatg gatgaaatga aactaattgg tatagattta
      121 gacggtactc ttttgaattc tgaccagagc attagtcaaa aaaatgcaag aacaatgaaa
      181 catttaccgg aagactgttt tccattcatt tgttctggaa gagaagtaga agacattaat
      241 aatattctca aaaaatctgg tttaaggata ccagcagtag ggttaaatgg ttctgttgga
      301 tttgatggag aaaaaaagat atttgagtct tccttcgata aaaatgatgt aaaaaaaatt
      361 gattctgtcc tatcgaaatt tccaaccaaa atatatacaa atctcggaag ttatgaatct
      421 cagaattata aaaataaaat gaaaaaaatt tttcacgagg tagggagtga attttctatc
      481 aaagagctaa actatg
//
LOCUS       DBKOIJ010000368          468 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000368 DBKOIJ010000000
VERSION     DBKOIJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.379"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>468
                     /locus_tag="ACV5NJ_003002"
     CDS             <1..>468
                     /locus_tag="ACV5NJ_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HHY6486990.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DBKOIJ010000369          421 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000369 DBKOIJ010000000
VERSION     DBKOIJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.380"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(170..355)
                     /locus_tag="ACV5NJ_003003"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACV5NJ_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DBKOIJ010000370          419 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000370 DBKOIJ010000000
VERSION     DBKOIJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.381"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..366)
                     /locus_tag="ACV5NJ_003004"
     CDS             complement(<1..366)
                     /locus_tag="ACV5NJ_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HHY6486991.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENII"
BASE COUNT          151 a           80 c           43 g          145 t
ORIGIN      
        1 aataatattt tctaaacacg ctgtagataa ctcttttgtt ccagttgttg tattatactt
       61 ataaatccac cagtctccca taacatcaga taaaggattt aaccaaattg ttcgattgat
      121 taatgttgta aataaaatca tcgcagtata gaatatcaaa gcacaaactt ttctatattt
      181 tctatccttc tttaaatgaa gataaagttc cattagtata cgtttatagc cctcacctgc
      241 agcaatgcca tctgttgcat acataaaata gaccagtcct aaaattgtca ataaaacagc
      301 aaacccaaag ggttcatata atttatataa tatatctgta ataatttctg caatcactat
      361 tatcataatt atttctccaa tatcaagtat acacttatcg cttccaatta cacatgatc
//
LOCUS       DBKOIJ010000371          411 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000371 DBKOIJ010000000
VERSION     DBKOIJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.382"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>411
                     /locus_tag="ACV5NJ_003005"
     rRNA            <1..>411
                     /locus_tag="ACV5NJ_003005"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DBKOIJ010000372          408 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000372 DBKOIJ010000000
VERSION     DBKOIJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.383"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            261..>408
                     /locus_tag="ACV5NJ_003006"
     CDS             261..>408
                     /locus_tag="ACV5NJ_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HHY6486992.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHE
                     QLDQL"
BASE COUNT          146 a           66 c           62 g          134 t
ORIGIN      
        1 ggttttagaa aagatttatt atacgtaaaa agctcaagac attttcttag ccataaggac
       61 atgtttgata tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca
      121 ttatcaacta aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag
      181 cttatagaag ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc
      241 tcttttccct ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg
      301 taaatgccca gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt
      361 ataaaaaaca agaagaaatt caaaaacatg aacaacttga tcaactcc
//
LOCUS       DBKOIJ010000373          387 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000373 DBKOIJ010000000
VERSION     DBKOIJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.384"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            145..>387
                     /locus_tag="ACV5NJ_003007"
     CDS             145..>387
                     /locus_tag="ACV5NJ_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HHY6486993.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          140 a           62 c           74 g          111 t
ORIGIN      
        1 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
       61 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
      121 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
      181 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
      241 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
      301 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
      361 tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DBKOIJ010000374          386 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000374 DBKOIJ010000000
VERSION     DBKOIJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.385"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>386
                     /locus_tag="ACV5NJ_003008"
     CDS             <1..>386
                     /locus_tag="ACV5NJ_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6486994.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DBKOIJ010000375          386 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000375 DBKOIJ010000000
VERSION     DBKOIJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.386"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>386
                     /locus_tag="ACV5NJ_003009"
     CDS             <1..>386
                     /locus_tag="ACV5NJ_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6486995.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DBKOIJ010000376          385 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000376 DBKOIJ010000000
VERSION     DBKOIJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.387"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            20..340
                     /locus_tag="ACV5NJ_003010"
     CDS             20..340
                     /locus_tag="ACV5NJ_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HHY6486996.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT          149 a           77 c           69 g           90 t
ORIGIN      
        1 cataaaagag gtgagcgcca tgaaattggt aaatgtaaca aacagccatt cccgtcttgt
       61 attaaaccag cttgaaaaca ctgatgccca tctagtcaag gtctacacag ccggaaatac
      121 cactattgtc tacactgaag cccctcaaca taatgaaatt ctgctgatca atgacaaacg
      181 taaaatccag ccgaaagaaa tcgaagatgc taaaaattat ttcttagata aaatcaaaga
      241 cgccgtttac cacgaggatg aaatcaaagt aatcgaattg gcaggccttg ttgaaatctc
      301 gattccaaaa catgaattag gcgctacatt agccgtgtaa acaaattttg aacataataa
      361 ttaaagagcg tgggacaaaa gtaaa
//
LOCUS       DBKOIJ010000377          370 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000377 DBKOIJ010000000
VERSION     DBKOIJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.388"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            82..>370
                     /locus_tag="ACV5NJ_003011"
     CDS             82..>370
                     /locus_tag="ACV5NJ_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HHY6486997.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          132 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa
//
LOCUS       DBKOIJ010000378          362 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000378 DBKOIJ010000000
VERSION     DBKOIJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.389"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..>362)
                     /locus_tag="ACV5NJ_003012"
     rRNA            complement(<1..>362)
                     /locus_tag="ACV5NJ_003012"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DBKOIJ010000379          362 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000379 DBKOIJ010000000
VERSION     DBKOIJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.390"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
BASE COUNT          136 a           59 c           80 g           87 t
ORIGIN      
        1 atatacgaat tataagatac attttatatt taatgtatgt aaataaataa aatgactgac
       61 ggatattgaa tagtaataaa taacaataat tcagacggat acagtgagtg aagtagcttg
      121 ctacgaacga acaggcagaa tgaaacggta gtttcattag agcaagtcgc ttgcgacgta
      181 gcgttaccac tgatgtaact ggcaagttac atagagagag tttgcttgca aacgaacgtt
      241 acccacttga atagaaaaca aaaataacat attagtgaat tttgaatagg agccacaact
      301 caaccaaccg cttgcggtgg gtgagtccca cggaggaaaa gacgaacgaa cggtcgcgta
      361 gc
//
LOCUS       DBKOIJ010000380          362 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000380 DBKOIJ010000000
VERSION     DBKOIJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.391"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..>362)
                     /locus_tag="ACV5NJ_003013"
     CDS             complement(<1..>362)
                     /locus_tag="ACV5NJ_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6486998.1"
                     /translation="KETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVN
                     LPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKG
                     VADIKVSTSYTVTTIISY"
BASE COUNT          101 a           70 c           53 g          138 t
ORIGIN      
        1 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
       61 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
      121 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
      181 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
      241 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
      301 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
      361 tg
//
LOCUS       DBKOIJ010000381          344 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000381 DBKOIJ010000000
VERSION     DBKOIJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.392"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>344
                     /locus_tag="ACV5NJ_003014"
     CDS             <1..>344
                     /locus_tag="ACV5NJ_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HHY6486999.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DBKOIJ010000382          343 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000382 DBKOIJ010000000
VERSION     DBKOIJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.393"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>343
                     /locus_tag="ACV5NJ_003015"
     CDS             <1..>343
                     /locus_tag="ACV5NJ_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HHY6487000.1"
                     /translation="LSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQ
                     SRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYK
                     RITVKEIANYFS"
BASE COUNT          121 a           44 c           64 g          114 t
ORIGIN      
        1 tctgagtaat ggaataattc caagtgttag tggagatacg attcggtctg aaaaaaatta
       61 ttctatcatt gtttttgaaa agttagcaca aacgtctata acattaggaa tggatataat
      121 agaagcgtat caaagtagag atgctttgat acaagaaaac gaattagctg tatcgttacc
      181 tgaggtctta aaagtgaggg attctgggat tgtttattat actaaggaaa tcggaaaaac
      241 taagatagag catctttctc ccttaatttc ttctgtcgtt caattcatcg gcttaaatat
      301 ctataaaaga attactgtaa aggaaatagc taattatttt tct
//
LOCUS       DBKOIJ010000383          341 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000383 DBKOIJ010000000
VERSION     DBKOIJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.394"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>341
                     /locus_tag="ACV5NJ_003016"
     CDS             <1..>341
                     /locus_tag="ACV5NJ_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HHY6487001.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DBKOIJ010000384          336 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000384 DBKOIJ010000000
VERSION     DBKOIJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.395"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            121..327
                     /locus_tag="ACV5NJ_003017"
     CDS             121..327
                     /locus_tag="ACV5NJ_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HHY6487002.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           81 g           92 t
ORIGIN      
        1 ataaaattga acaactggac gggcaaattg atgccttgct ttcagaaaga gaaatattga
       61 tagaagaaaa caagcgattg aaaaagaaag tagcccaacc aatgaaggga ggtggatagt
      121 atgaacaata gaacctttga tattttgaaa tggatcgcta tagtagttat ccccgcttta
      181 gcgacttttg tcggtcttgt cggaaaaggt gttaactggc ggtacacaga tataacggtg
      241 atgattatta caggttttgg agcatttcta gggagtattt taggtgtatc aaatcgaacg
      301 tataagaaaa tttcatcaga taaataggag gataga
//
LOCUS       DBKOIJ010000385          323 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000385 DBKOIJ010000000
VERSION     DBKOIJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.396"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACV5NJ_003018"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACV5NJ_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HHY6487003.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DBKOIJ010000386          321 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000386 DBKOIJ010000000
VERSION     DBKOIJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.397"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>321
                     /locus_tag="ACV5NJ_003019"
     CDS             <1..>321
                     /locus_tag="ACV5NJ_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HHY6487004.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DBKOIJ010000387          285 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000387 DBKOIJ010000000
VERSION     DBKOIJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.398"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            1..285
                     /locus_tag="ACV5NJ_003020"
                     /pseudo
     CDS             1..285
                     /locus_tag="ACV5NJ_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          110 a           58 c           56 g           61 t
ORIGIN      
        1 acaacaaatc gtcgatctct atctcgctgg taagcctcgc gcagaaatta ttcgagagta
       61 tgagcttacg ccttcttctt tcgataaatg gatgaagcaa gcacaatcaa cgggctcatt
      121 caaagaaaga gacaacttaa caccagaaca agcagaattg atcgcactaa gaaagaaaaa
      181 taagcaactc gaaatggaga atgatatttt aaagcaagcg gcgctgatat tcggacgaaa
      241 agacaagtaa ttgatgccaa caagcataaa tattccatat cagcg
//
LOCUS       DBKOIJ010000388          268 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000388 DBKOIJ010000000
VERSION     DBKOIJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.399"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(36..>268)
                     /locus_tag="ACV5NJ_003021"
     CDS             complement(36..>268)
                     /locus_tag="ACV5NJ_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6487005.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           79 a           39 c           47 g          103 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaata
//
LOCUS       DBKOIJ010000389          266 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000389 DBKOIJ010000000
VERSION     DBKOIJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.400"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>266
                     /locus_tag="ACV5NJ_003022"
     CDS             <1..>266
                     /locus_tag="ACV5NJ_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HHY6487006.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLR"
BASE COUNT           92 a           39 c           51 g           84 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaa
//
LOCUS       DBKOIJ010000390          265 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000390 DBKOIJ010000000
VERSION     DBKOIJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.401"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
BASE COUNT          123 a           32 c           40 g           70 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaa
//
LOCUS       DBKOIJ010000391          235 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000391 DBKOIJ010000000
VERSION     DBKOIJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.402"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
BASE COUNT           97 a           26 c           44 g           68 t
ORIGIN      
        1 tgacgcactt ttgatgtcat gttataataa cgaagtcgtt tggatgagat agctaataaa
       61 gcttgatttt atcgaaaaaa acttcgaaaa agttattgac aaataacaat aaagatggta
      121 tgataatcaa gtcgctgcta atgagtggca aagacgttta gaaaaaataa acaaagaagt
      181 tgttgacatt ctcttctgaa tctgataaga ttaaagagtt gttaaaacaa aaaga
//
LOCUS       DBKOIJ010000392          232 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000392 DBKOIJ010000000
VERSION     DBKOIJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.403"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..>232)
                     /locus_tag="ACV5NJ_003023"
     CDS             complement(<1..>232)
                     /locus_tag="ACV5NJ_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6487007.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DBKOIJ010000393          232 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000393 DBKOIJ010000000
VERSION     DBKOIJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.404"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(<1..>232)
                     /locus_tag="ACV5NJ_003024"
     CDS             complement(<1..>232)
                     /locus_tag="ACV5NJ_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HHY6487008.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDH"
BASE COUNT           79 a           40 c           36 g           77 t
ORIGIN      
        1 gatgatcgct acgtgcgtcc ggcacgtcag tcacgatatc aaacaaaaag tatacatcct
       61 tcttcattct agttttcgca gcaggaattt tgaaatagtt cttattagaa tagtagagat
      121 tgattaataa gctatcttcg attgctaaaa agaaaacttc tgaattccat actttataaa
      181 aatctttgat aaatctattc gaagggtcaa atttaaacca taattgtgtc tc
//
LOCUS       DBKOIJ010000394          224 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000394 DBKOIJ010000000
VERSION     DBKOIJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.405"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>224
                     /locus_tag="ACV5NJ_003025"
     CDS             <1..>224
                     /locus_tag="ACV5NJ_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HHY6487009.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DBKOIJ010000395          220 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000395 DBKOIJ010000000
VERSION     DBKOIJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.406"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>220
                     /locus_tag="ACV5NJ_003026"
     CDS             <1..>220
                     /locus_tag="ACV5NJ_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HHY6487010.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRKL"
BASE COUNT           66 a           40 c           45 g           69 t
ORIGIN      
        1 tcttttattg cgaaaaattg tcaggtatct ttgaccaccg ttattcgtac gttgaaagag
       61 tttaaaagct acttaccaaa gcaatctaag aggattctcc ccagagtatt gatggttgat
      121 gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc agatggcgaa
      181 acaggacaat taattgatgt cttgcctacg cgtaaattgc
//
LOCUS       DBKOIJ010000396          207 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000396 DBKOIJ010000000
VERSION     DBKOIJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.407"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>207
                     /locus_tag="ACV5NJ_003027"
     CDS             <1..>207
                     /locus_tag="ACV5NJ_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HHY6487011.1"
                     /translation="AVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSISNTNDID
                     IYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           74 a           37 c           36 g           60 t
ORIGIN      
        1 ggcagttgat attgaaaatt cgattaatga atggcttcaa tccgtaccca aaaatctttt
       61 caaatcaatt acctttgatt gtggaaaaga attctctaat tggaaaagta ttagtaatac
      121 gaatgatatt gatatttatt tcgcagaccc aggaacgcct tcccaacggg gattaaatga
      181 acattcaaac ggactcctcc gaaaaga
//
LOCUS       DBKOIJ010000397          206 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000397 DBKOIJ010000000
VERSION     DBKOIJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.denovo.408"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..>206
                     /locus_tag="ACV5NJ_003028"
     CDS             <1..>206
                     /locus_tag="ACV5NJ_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HHY6487012.1"
                     /translation="VKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNN
                     LIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT           74 a           44 c           37 g           51 t
ORIGIN      
        1 gtgaagcagt ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt
       61 gcctttaaga aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa
      121 tacctgaata atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat
      181 cgaagtttac gcactgcctc taccac
//
LOCUS       DBKOIJ010000398        16339 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.053, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000398 DBKOIJ010000000
VERSION     DBKOIJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.053"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(134..577)
                     /locus_tag="ACV5NJ_003029"
     CDS             complement(134..577)
                     /locus_tag="ACV5NJ_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HHY6487013.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACV5NJ_003030"
     CDS             complement(795..2135)
                     /locus_tag="ACV5NJ_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HHY6487014.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACV5NJ_003031"
     CDS             2404..3300
                     /locus_tag="ACV5NJ_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HHY6487015.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACV5NJ_003032"
     CDS             complement(3450..4391)
                     /locus_tag="ACV5NJ_003032"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HHY6487016.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACV5NJ_003033"
     CDS             4517..5638
                     /locus_tag="ACV5NJ_003033"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HHY6487017.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACV5NJ_003034"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACV5NJ_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HHY6487018.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACV5NJ_003035"
     CDS             7465..7992
                     /locus_tag="ACV5NJ_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HHY6487019.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACV5NJ_003036"
     CDS             8203..9264
                     /locus_tag="ACV5NJ_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HHY6487020.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACV5NJ_003037"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACV5NJ_003037"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HHY6487021.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACV5NJ_003038"
     CDS             10177..12159
                     /locus_tag="ACV5NJ_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HHY6487022.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACV5NJ_003039"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACV5NJ_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HHY6487023.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ACV5NJ_003040"
     CDS             12881..14305
                     /locus_tag="ACV5NJ_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HHY6487024.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="ACV5NJ_003041"
     CDS             complement(14486..15259)
                     /locus_tag="ACV5NJ_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HHY6487025.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="ACV5NJ_003042"
     CDS             complement(15256..16161)
                     /locus_tag="ACV5NJ_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HHY6487026.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
BASE COUNT         5245 a         2966 c         3392 g         4736 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatg
//
LOCUS       DBKOIJ010000399        15588 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.059, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000399 DBKOIJ010000000
VERSION     DBKOIJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.059"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            143..625
                     /locus_tag="ACV5NJ_003043"
     CDS             143..625
                     /locus_tag="ACV5NJ_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HHY6487027.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(888..1355)
                     /locus_tag="ACV5NJ_003044"
     CDS             complement(888..1355)
                     /locus_tag="ACV5NJ_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HHY6487028.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            1478..2329
                     /locus_tag="ACV5NJ_003045"
     CDS             1478..2329
                     /locus_tag="ACV5NJ_003045"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHY6487029.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(2580..4538)
                     /gene="nagE"
                     /locus_tag="ACV5NJ_003046"
     CDS             complement(2580..4538)
                     /gene="nagE"
                     /locus_tag="ACV5NJ_003046"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HHY6487030.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(4535..5407)
                     /locus_tag="ACV5NJ_003047"
     CDS             complement(4535..5407)
                     /locus_tag="ACV5NJ_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HHY6487031.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(5749..6621)
                     /locus_tag="ACV5NJ_003048"
     CDS             complement(5749..6621)
                     /locus_tag="ACV5NJ_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HHY6487032.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            6749..7318
                     /locus_tag="ACV5NJ_003049"
     CDS             6749..7318
                     /locus_tag="ACV5NJ_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HHY6487033.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            7385..8122
                     /locus_tag="ACV5NJ_003050"
     CDS             7385..8122
                     /locus_tag="ACV5NJ_003050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HHY6487034.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            8353..9621
                     /locus_tag="ACV5NJ_003051"
     CDS             8353..9621
                     /locus_tag="ACV5NJ_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HHY6487035.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            10108..10572
                     /locus_tag="ACV5NJ_003052"
     CDS             10108..10572
                     /locus_tag="ACV5NJ_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HHY6487036.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            10556..11185
                     /locus_tag="ACV5NJ_003053"
     CDS             10556..11185
                     /locus_tag="ACV5NJ_003053"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HHY6487037.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            11539..13017
                     /gene="msr(C)"
                     /locus_tag="ACV5NJ_003054"
     CDS             11539..13017
                     /gene="msr(C)"
                     /locus_tag="ACV5NJ_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HHY6487038.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            13064..13510
                     /locus_tag="ACV5NJ_003055"
     CDS             13064..13510
                     /locus_tag="ACV5NJ_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HHY6487039.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(13709..14224)
                     /locus_tag="ACV5NJ_003056"
     CDS             complement(13709..14224)
                     /locus_tag="ACV5NJ_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HHY6487040.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            14341..15542
                     /locus_tag="ACV5NJ_003057"
                     /pseudo
     CDS             14341..15542
                     /locus_tag="ACV5NJ_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         4896 a         2800 c         3229 g         4663 t
ORIGIN      
        1 ttatttccaa atggctaagt taattataaa atccgccaaa tagttaaaaa ataaaaatat
       61 ccctagaaaa aagagcgtaa tcgtgtcatt tccctttaat tttatgtaag atgaacttaa
      121 tataatagaa aggatgggag aaatggcaaa gtataggatc gaagaaaaac cagattttac
      181 ggtgacgggt attggtgtgg aattgaagag tagcaatacg gattttgtcg gtttggctgc
      241 agagaaacaa gcatttgagc aaaaagttat cggtaatgga acaatagatg cattgaaaca
      301 agctgctgaa aatcattatt tctttacagt caacgaaccc tataacggga aaatgatgta
      361 ctatgtcggt gttcaaacga gtcaggatgt tccaaacact acgcgaatca tcccattccc
      421 aaaaggaacg tatcttgttg taagcgcttt agcagaatcg cctgaagaat taagaaatga
      481 tttgacgaat acggcctttg ggcatgtttt aagagaagta gaagattacg cctatgttgg
      541 gggtccaaat gctgtagtcg aaatgggagt taccaacggg aaatatatgg gagaaatctg
      601 gataccggta gtcaataaac catgataata tagaggtcgt gaaagtagcg ctttgacctg
      661 agcattagag aggaaaaatg ggaaatagct ttccatattt tgcatttttc agaactaatg
      721 ccgagggtca gctacttgaa caccgtttat caagacgtca tgacttcagc gctttgacct
      781 gagcattaga gaggaaaaag gggcatgaca aggcttttgt cccccggaga ataaaagaat
      841 ttatgtagac gattccgatc agaaaaatgc aggcacagta gagagaatta taaaaatctc
      901 tctacgaggc ctgtgttttc tggtcttttt tctttttcca aaacggctat tttatgtatc
      961 agactatcaa gtgtgatctt agccatttct tcttctgcct ttttctgcag ccgagcgtag
     1021 ctttcttcta aagcttcttg gatattcgca ccaacgacgc aatttggatt tgtttttgga
     1081 tcgacttgga taagctgtgt attaccttcg atgctgcgat agacatctag taaagaaatc
     1141 tgatctggac ttttggcaag ggaaggctgc ggttttccgg tttttgtaaa gatcaaaccg
     1201 gattttttga gctgactcat gattttacgt acatttgctg catttgtttc gatactactg
     1261 gcaatcatct cactagaaag tgatcgggta tcttgaaaca tttcaatata ggcaagtaca
     1321 tggattgcat cactaaattg cagagaatac ttcatcagtt gtcatccttt catttttttt
     1381 gcttgactta tcttaccaca ctctttatga taaaggtgta atttataaat gtacagctgt
     1441 aaatcttaac aaataaaaag aatagggagt ggcaaatatg cattatttag taacaggagc
     1501 aactggaggt tttggtggtt atgcgctgga attcttaaaa aagatggttc cacaaaatga
     1561 aatctatgta ctagtacgta gtgaagaaaa aggagaagca ctgaaagaag ctggatttaa
     1621 tatacgtatt ggtgattatg atgatttaga ctcaatgaaa gaagcgttga aaggaataga
     1681 tcgattgctt tttgtctctg gcgttccagg aaatcgtcaa gccgaacata agaatgtagt
     1741 gaaggcagca caagcagctg gggtatctta tatcgcttat acaagttttg cagatgccga
     1801 tcactcgatc agtgtattag cgccagatca ccaattcact gaaaaaatga ttaaggattc
     1861 aggtatttcc catacatttt tacggaataa ttggtatgta gagaatgaat tacctatcat
     1921 tgggcaggct ttaaaaactg gtcaattcgt ttatgctgca ggaaatggaa aaacaggttg
     1981 ggcattgaaa cgtgaatatg cagaagctgc agcaaaagta ctagtagaaa gtgactctcc
     2041 agaaatctta gaattatccg gcaatctaac tgactatgaa gaattagcca aggcattaga
     2101 gcgtgcgaca ggtaaagagt tagaaattat tgaagcaagt gatgcagcgt ttgtcgaaaa
     2161 tctaaaagaa gccggattcc ctcaagaagc agctgacatg tttttagcga tccaacatga
     2221 tattaaaaag gatcagttag ctatttcctc tgatgattta gaaaaagtat tgggaaaacc
     2281 attaacttct cttgaagcag cattaaaaga aatattggat caggaataat gtataaagag
     2341 gttgtgaaaa agccgattag ttttgagtat gaagaaaaat tttgtgaaaa agctgttctg
     2401 catcaagtaa taagaagagt caaataaaaa atagctcttc atatgtttcg tttagctgga
     2461 cttattaccg cggagttggc aggagaacgc cgtttttatt cggaaaaagg agctgagaca
     2521 agatttttgt ctcagctcct gtttgtttat cgataaaaaa gtctggggaa gacgatggct
     2581 caggctttgt tggaaacaga gccgatttta tcatttgctt ttacatcacg tgcgggttcg
     2641 atttctaagc tttggacttt gtcgccattt gtgaatacaa cgaccatatc cgtgtttttg
     2701 cctgcagatt gaatagcggc aagatcaact aaagcaatca attgacctct tgcgactttt
     2761 tgaccttctt ctacatataa agtgaatggt tcgcctttta agtcaacagt gttgatgccc
     2821 atatgaagca agacttcgat accagcatct gtttggattc cgacagcatg tttcgttggg
     2881 aaaatgtttg tgatcgtacc agcgattgga gagaagattt ctccattttc tggtaataca
     2941 gcatagccgt cacccatcat tttttccgcg aatacatcat ctgaaacttc agtgattgga
     3001 atcagtttcc catttgcgat agcgtaaata tcttgttcga tcgtttcacc ggcaaatgtt
     3061 tctgttttaa ccacggtgtt tgtttgtgca ggtcgtgctg cagcaccacc atgtagtttt
     3121 gccatctcat ctgcaacgaa ttgtacttct gtacctacga tgacttgcgc attttttccg
     3181 tcgatgatct tcataccagg tacgccagtg gctttgattt tattttgatc gatcaggcct
     3241 gtatctttga cttgcaaacg aaggcgtgta gtacagttat cgatcgaagt tacattgtcg
     3301 gctccgccta atgcagcgta gattttgctt gcttgtgccg catatttatt gtctgaatta
     3361 gtcgttgcag tatcgtcatc tgaaagatct gcttcctcgc ggccaggagt catcagatgg
     3421 aattttttga tagcaaagtt aaagccaaag tagtaaatca ctgctgttac taatccttgg
     3481 acgatcaaca tcaatggctg attcgcaatc ggatttttca aagataggaa gtaatcgaca
     3541 aatccggcac taaaggcaaa tcctgctgtc caatggaaga atgcactcac tgctaaagaa
     3601 atcccagtga aaattgcatg caagacatat aatggccaag caacgaacat aaatgaaaat
     3661 tcaagtggtt ctgtcacacc agtaaagaat gaagcaaaag ctgctgctag catcaaggaa
     3721 gctgtttttg ctttgttttc tggacgtgca tttctgtaaa tagcatatgc tcctgctgga
     3781 agcccgaaca tcatgattgg gaaaaatcca gcttgataca ttcctgttac gccttttgtt
     3841 ccttcagaag ccaaaaattt accaatatca ttgattcctg ctacgtcaaa ccagaacacg
     3901 gagttcattg cttgatgcaa tcctgtaggg atcagtaaac ggttgaagaa gccataaaga
     3961 cctgcaccac cccagcctaa actcaaaatg aatttagcaa aagaaactaa agcagagtaa
     4021 accggaggcc atacgagcag tagcactgca ctgatcagca acatgacaaa ggcagccaag
     4081 attggcacta gacgcttgcc gctaaagaaa gataaagcca tcggtaattt tgcttcgtgg
     4141 aaacgattat aaagagctgc cgcaatcaat ccggcaataa taccaataaa tacgttactg
     4201 atctgagcaa aagatggatc aactgattct ggtttgatat tcaataaagt agctactgaa
     4261 tcagttttta aaacatttac tggaacttca aatgcgacca aacctgccaa ggcagcagca
     4321 cctgatttgt ccactgacat ccctagtgct aacccaacgg caaacagcaa aggtaattga
     4381 cctaaaactg cgttaccgcc agcttgtaaa aaatgagcga caatgtcgtt actataagat
     4441 gcccaccagt ttccgatccc tactagtaaa gcagcagcag gtaagaccgc aactggaagt
     4501 tgaagggaac gtcccattcg ttgtagatat gctttcaatt tttttcctcc tcatgattgt
     4561 taacatttgt ttgtacgatt gcgcttctta aacgctcgat atggatggcc aagtaaccca
     4621 gttcttcttt cgtgatcgcg agctggtatt ctttggtcag caacacacgg atttttttag
     4681 aaatcttata gctttctggg tattttcgtt tgaccatttc tacgatttcc gtgtctaaaa
     4741 cagcatattg ttgcgcatgg gttcgttgta gaagcagtcg taaatgattc accagtcttg
     4801 aataattcag tgaaaatggt tctgaatgga tatctgtttc caattctgat tccactaaat
     4861 ggatgatgtc agatacaatc gtcacttcac ggatactgcg atggttattg ttccgaccgc
     4921 ttcgtccgct gtgaatatgg attgcgatat atcctgcttc gtcataagta tatggaatcc
     4981 caagctcttt tgtcagatac tctactgccc attgagcaat actgaattca tcactgtaaa
     5041 gcacttcgat ttctcttaaa agtttgttgc ggatgacgat accgttatta atgttctccg
     5101 cagaaaaagc aatgtggtcg gtcaaagcga tcaatacatg ttcattcaac ttttccatca
     5161 aaacagtttc cgcatgatca atgatcgttt ctgctgcaaa taaaaagcgt tcatcaatct
     5221 gattaagtaa attttgcaac ttgatttgac cttctggttc catgacaaat atccgttcga
     5281 catcacgatc aaaaatcaaa tcatttcttt ttctgtcaaa tccaatccct tttccaatag
     5341 ctactttttc ctgtccaccg tcatctacca atactgcatt ttgattaaag atctttttga
     5401 ttttcatgcg cgcacccttt ttgaagattt ggttttcacg tttgctagac cagttgattg
     5461 cggccaaaca aaaatagaca cgaaaaacct cctagggaaa ccatgttcct cagagatttt
     5521 tcatgcctag tatgatctag tcacatgaaa cgaattattg ttaacgtaat caattttata
     5581 tagacagtta tgttttgtca attctttttt tataattata gtaatttact ggaattatca
     5641 gaaataaaag gaagaaataa attaagaaac ttatgttatt taatcgttgg atcttttgct
     5701 tatcttattc gttgttaaaa agctgaaagc gaaggaaaaa aactctactt aactgtattt
     5761 ctccgttagg acatctaaaa atttacgagc agcaggagaa agaggctggt cttttttcca
     5821 aatgagattc agtgaagctt gtaatggcgg atcgagtggg atgaatattt tttccgtccc
     5881 ataagtattg atgatcccat ctagagctaa tgcaccaaaa gtcccgttat cgaccatcaa
     5941 agaagcattg tacaataagt tatatgttcc gacgatcgat aactgatcta tgctgaaacc
     6001 tagccattca gctagttgac tgtccactga cgtttgccgg gaaatgatca aaggaacagt
     6061 tttcagttcc tgcggatgga tgacggtttg ctttgctaaa ggatgatttc gatgtatcaa
     6121 aagtccccaa cgatcttggg ctggcagtcg aaatgactgg tatttttgtt tgtttgtcgg
     6181 ttcgattacg agtccaaaat caagtaatcc gctatccagt ttgtctatga tatcatctgc
     6241 attcccgcta tataaatgaa actggatatc aggatgcgcc ttgatcaacg aatgaaggct
     6301 atcagaaatg aaagagattg ctttagtttc gcctccgcca atgtagattt ctccagagat
     6361 gattgcgtca tttgttaaat tgttcgccgt tttttctacc aacgacaaga tttcttttcc
     6421 ttttgaataa agatatcgac cagaatccgt caactgtatc tctcgggagc cccgagtgaa
     6481 aagtgtcaca ccaagttctt cttctaaatc tttcagttgt tttgagagag taggttgcgt
     6541 gatatgcaga gattttgctg ctcggctgat atttttttct ctagctactg ctaaaaaata
     6601 cgtcagtata cgaatatcca ttttttccct cctgttttta aaaaagcata ccactaagta
     6661 tagttaaaag gaatagttaa gtatgtttta taagtattag acatgataag ccgtccgtta
     6721 taaagtaagg ataaaaggag ggagcaaaat gacaagcctc atcgaaaaac aaatcgtcaa
     6781 taaagagatt cgagtaacgg atccgctctt tgaagtgatc catcagatcc aagctgaaaa
     6841 cgaagagcct ttagcagcgc tgaatactgg gtaccatgaa ccagcagata tccgcaaacg
     6901 gctagaaaat atcattagtg acaaagtaga tgatacggtg actgttctgc tgccttttta
     6961 tacagatttc ggcaaacata ttagtatagg gaaaaatgtc tttatcaatc gtcaagtgat
     7021 gttcgttgac ttaggtggca tctgtttaga agattcagta ttgattggtc ctcgtgtcaa
     7081 cctcatcacg gtgaaccatc taaccgatcc aaaagaacga agaggcttgt cagtaaagcc
     7141 aatccacatc aagaaaaatg cttggatcgg agcaggagca actattctgc caggcgtaac
     7201 gattggcgag aatgccattg ttgcagcgaa tgcaactgtt acgaaagatg tcccagacaa
     7261 taccatcgtt gcaggaatcc ctgccaaaat cgtcaaacct gtcgaacgga ttatttgaac
     7321 agtaagatga aataagagat agtgagtata agcaagagat ttttcaaaac aggaggagaa
     7381 tagaatgaat gatttatcag aaaaagtgat tgtcatcatg ggcgcttcaa gcggaatcgg
     7441 tgaagcaaca acgaagctat tagcagaaaa aggggctaaa ctagttatag ctgcacgccg
     7501 agaagatcgc ctaaaagcaa taaaagaatc tttaccagaa gctgagctat atattcaaac
     7561 agcagatgta agagattttg cacaagtcca agcagttatc gatttagcga tggaaaagtt
     7621 cggtcgaatc gatgtattgt acaataatgc aggaatcatg ccaaccgctc cattagttga
     7681 aggtcaccgt gatgaatggc aaaatatgct tgatatcaac atcatgggtg tgttaaatgg
     7741 aatttccgcc gtacttccaa tcatggaaaa acaaaaatct gggcatatca tttcaactga
     7801 ttctgtggcg ggacatgttg tttatcctga ttccgccgtt tattgcggaa caaaatttgc
     7861 tgtccgtgca atcatggaag gattacgtca agagcaacgg caaaataaca tcaaatctac
     7921 gatcatctct ccaggagctg ttcaaactga attatatcaa accatcagcg acaaaaaggc
     7981 tgctcttgaa cttcatgaag cgcaaaaaga atggggattg acctccgaag atatagcttc
     8041 tgctgtagca tttgcgatag aaacgccaga ccggatgtct gtcagtaaca tgattatccg
     8101 tccgacgaca caggaagtat aatccataac ggataatatc caaagagaat tacttgttaa
     8161 atagttattc aaacgaccat caatttaagg cgtgaatgga gaagaatatt cttcatttac
     8221 gtctttttat gtagaaaaag caaatttatc aaaaagaaac aaatgttatt taaatatgga
     8281 cattgacttg cttttacttt tttagttaca ctgttattta ggaaaagttt cataattcaa
     8341 aggagaaaaa agatgaaagc aaagaattta ttatttagcg ttattctttt aggaaatcta
     8401 gcagttgcca acacaacagt gctagctgaa gaatcgacag aaactgtcgc aactcagaca
     8461 tcgagttctt cagaaacagg aattgaaaac gatacagaga gtactttgca gtcagctact
     8521 gtctcagaaa atacaatttc aaccgaagaa ccaacaactg aaacaacaga acaaacacaa
     8581 accgaagaca gtacatcagc agaagctcag aacaatccag agaatgcaga agaaatcacg
     8641 aaccaattga cagatgggca aacattgaca catacccatc aagcagattt gtctgaattc
     8701 gaaaactata ctggctatgc tttttatgct cgctcatctg ctaattctca acaagcgttc
     8761 attgatagta ttgcctcaac tgcacaaagt ttggcaagtg ccaatgactt atatgcatca
     8821 gtcatgattg ctcaagccat cgttgaaagt ggctggggga acagtacttt ggcttctgcc
     8881 cctaattaca atctatttgg gattaaagga agctataatg gccaatctgt tacgatgcct
     8941 acttcggaat atgtgaatgg gcaatggatt acggtgaatg ccgcttttag aaaatatcct
     9001 tcgtacaaag aatcattgca agataacgta acggtattga agacgacttc ttttcagcca
     9061 ggcgtttact actattccgg tgcatggaaa agcaatacga actcttacaa agatgctacg
     9121 gcttggctaa caggtcgtta tgccactgcg cctaattatg gttctacatt gaacaatgta
     9181 atcgaaacct ataatctgac acagtatgat acagcaccaa cttcaacttc tccgatgtat
     9241 cgtctgtata accgtcatac aggagaacat ttatatacat tgaatgccgg agaaaaaaat
     9301 tatctgccaa ctgtaggctg ggaatatgaa gggattgctt ggcaagcacc aaacagcgga
     9361 caacctgtct accgtttata caatccttac agtggagacc atcattacac aatggcacaa
     9421 tctgagatca actctttaac aaaaatcggt tggcgttatg aaggtctaag tttttattct
     9481 ggtggttcca agccaattta tcgactattt aatccaaatg aaaaaactgg tacccatcac
     9541 tataccttga gtgctaagga aagagattgg ctaacaccga tgggctggag atatgaagga
     9601 ataggatttt atggttatta aaaagaggta gtgaaaaagc tgccctgcat caagtagtaa
     9661 gaacagccaa acaaaaacag attttcatgt tttttgtttg gttgaactta ttaccgcaga
     9721 tgcaagtttt tgaacaccgt ttactcaaaa aaggttgtga caacatcttg tcataacttt
     9781 tttattactg tttaagcata ctgatccaat ttctaatatt cgacgggatt cttgcaaaag
     9841 gcgatttcgg aaatttttgg cggtgtaaaa ttttctactg cagaagagct atcttctata
     9901 gtttgataag aatcaataat cttggtttta gcgttgtgct ttctttatca ttttttccgt
     9961 tcattctaca aaaaatttct tttctctata cttttctatt gagagcgttt tttttattac
    10021 acagctgatt ctttgacttt tttctttttt tattctatta ttgacaagtc aatcgttgac
    10081 gagtcaatta ttagaaagga ggaaattatg tctttagaag agattagtca ccttttatat
    10141 caaatcaaat tagccgatca aaaaatcgtc cagatctttg aagaaaatat cggtataagt
    10201 cttactagat acgagatcat gatgagactg aaagaagaaa atccattgct acaaagcagt
    10261 ttgcaagaaa agttgcagat tgatcaagga gcagttactc gtcatctgaa ggtattggaa
    10321 gcaaatggct atgtttcgcg tcagagaaat cctcagaaca accgggaagt gtatgtagcg
    10381 ctaacagaaa aagcccagaa gaaaatcgag cattgtgaaa tggaccataa agatacaggt
    10441 tccgtgctat ccccggcttt cagcgatgta gaaatgaaac aatttctaac acttttagat
    10501 aaattgaata cccaactttg caatataaca acagaaacga aagaggttaa taataatgga
    10561 acaaaaaaat gatttttcaa caattatgac tggtagaaga tccgttcgcg tatatgatga
    10621 aacatacaaa atcccacacg aagaaatgct ggaaatgatc aaagaagcga cgactgcacc
    10681 ttcttctgtc aatatgcaac catggcgctt tgtggtcgct gaaagcgagg aagcaaaaga
    10741 aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt ctgcaatggt
    10801 catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctata accaagctta
    10861 cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga tcattcctta
    10921 ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt ctagcttagc
    10981 ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc caattggtgg
    11041 attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt atgttccagt
    11101 tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc gtttagcacc
    11161 agaaaagatc actacgttcg aataaaaggt tttcagttac tattcagtag ctattgcttt
    11221 ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg
    11281 aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat gtataaacaa
    11341 caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg tgaaagctat
    11401 gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag
    11461 tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga
    11521 aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc
    11581 tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata
    11641 gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa
    11701 ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta
    11761 acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga
    11821 ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata
    11881 cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat
    11941 agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt
    12001 gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac
    12061 ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag
    12121 aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa
    12181 tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa
    12241 aagcaacaaa tcagaccgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa
    12301 gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc
    12361 aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc
    12421 cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta
    12481 aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca
    12541 ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg
    12601 aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc
    12661 attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat
    12721 cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa
    12781 gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat
    12841 gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata
    12901 tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat
    12961 gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg
    13021 tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa
    13081 agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta
    13141 taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg
    13201 attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc
    13261 ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt
    13321 gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat
    13381 acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt
    13441 accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag
    13501 taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc
    13561 tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg
    13621 atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc tcttcaatag
    13681 atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt tgttctctta
    13741 cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg atcacaacat
    13801 aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt tgattttttt
    13861 gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct gttgttttat
    13921 cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta ggagcgggag
    13981 tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag tggtagatct
    14041 ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg tctaacttgg
    14101 caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc agctgcttca
    14161 attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag gctaaagtat
    14221 tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa gtttccacgt
    14281 tttacatgtg catatttaca tgtaaaataa atcatgtatg atgctaatag agagaaaggg
    14341 atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga aacaaagggg
    14401 aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg agtgggtcta
    14461 gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc tgaaacgacc
    14521 ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac aggtttctta
    14581 tctagtcgga tcggtttgaa aaaacattga tgacaggatt gtttatcatc gttgtattcg
    14641 ctggtcttgg cggattatct gggaatatcg gaatgctgat tgggttacgt gccggctggg
    14701 gtgtcgggaa tgctttattt gtctctactg ctttagcaac gatcgtcagt attttagttg
    14761 gtaacacaga aaaagcaatc atgatgtatg aagcagcact tggctgcgga atggctgttg
    14821 gccctctagt cggtggggtc ttgggcagtg gttcatggcg ttatccattt tttggggtag
    14881 catttttgat gttttgcggt tttatagccc tagcggtact tttacctgaa actgagaaac
    14941 caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt
    15001 cagttggact aatcgcattg ttatataatt tcggattctt tactttactt gcgtatgcgc
    15061 catttttatt gattggctat tctgaattag aagtcggttt tgtatttttt ggctggggtg
    15121 tgcttctggc tattgcttcg attttcgttg caccggctct tgagcggaag tttacgactt
    15181 atcggacaat tttagcagca tttgtcttat tcattatttg tttgctgtta ttaggagctg
    15241 gaacttctat gccagtcctt gtaccgatca gcattgtgtt agcaggattt ttccaaggaa
    15301 ttatcaatac actgttgacg acgattgcta tggaaatact aggtctgcaa cgaaacgttg
    15361 cttcgtccag ctatagtttt gtccgctttt tcggtggagc tttagcacca tttattgcag
    15421 gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt gccattgttc
    15481 ttatcagtgt atgttgtatc atttatcacc gtaattattt tgctacaaat gaagaagaat
    15541 aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DBKOIJ010000400         7052 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.139, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000400 DBKOIJ010000000
VERSION     DBKOIJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.139"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            260..1171
                     /locus_tag="ACV5NJ_003058"
     CDS             260..1171
                     /locus_tag="ACV5NJ_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HHY6487041.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACV5NJ_003059"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACV5NJ_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HHY6487042.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="ACV5NJ_003060"
     CDS             2328..2612
                     /locus_tag="ACV5NJ_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HHY6487043.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2978..3277
                     /locus_tag="ACV5NJ_003061"
     CDS             2978..3277
                     /locus_tag="ACV5NJ_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6487044.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3320..4810
                     /locus_tag="ACV5NJ_003062"
     CDS             3320..4810
                     /locus_tag="ACV5NJ_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HHY6487045.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            5158..5328
                     /locus_tag="ACV5NJ_003063"
     CDS             5158..5328
                     /locus_tag="ACV5NJ_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6487046.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            5342..5959
                     /locus_tag="ACV5NJ_003064"
     CDS             5342..5959
                     /locus_tag="ACV5NJ_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HHY6487047.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            5959..6906
                     /locus_tag="ACV5NJ_003065"
                     /pseudo
     CDS             5959..6906
                     /locus_tag="ACV5NJ_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         2593 a         1045 c         1334 g         2080 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc
     3181 gattagtgga aaaaaattga cttataaagg aaaaaaatct ttttcaaaac atgcaatatt
     3241 gaaacagttg aatgaaaaag caaaccaagt taattaaaca acctatttta taggatttat
     3301 aggaaaggag aacagctgaa tgaatatccc ttttgttgta gaaactgtgc ttcatgacgg
     3361 cttgttaaag tacaaattta aaaatagtaa aattcgctca atcactacca agccaggtaa
     3421 aagcaaaggg gctatttttg cgtatcgctc aaaatcaagc atgattggcg gtcgtggtgt
     3481 tgttctgact tccgaggaag cgattcaaga aaatcaagat acatttacac attggacacc
     3541 caacgtttat cgttatggaa cgtatgcaga cgaaaaccgt tcatacacga aaggacattc
     3601 tgaaaacaat ttaagacaaa tcaatacctt ctttattgat tttgatattc acacggcaaa
     3661 agaaactatt tcagcaagcg atattttaac aaccgctatt gatttaggtt ttatgcctac
     3721 tatgattatc aaatctgata aaggttatca agcatatttt gttttagaaa cgccagtcta
     3781 tgtgacttca aaatcagaat ttaaatctgt caaagcagcc aaaataattt cgcaaaatat
     3841 ccgagaatat tttggaaagt ctttgccagt tgatctaacg tgtaatcatt ttggtattgc
     3901 tcgcatacca agaacggaca atgtagaatt ttttgatcct aattaccgtt attctttcaa
     3961 agaatggcaa gattggtctt tcaaacaaac agataataag ggctttactc gttcaagtct
     4021 aacggtttta agcggtacag aaggcaaaaa acaagtagat gaaccctggt ttaatctctt
     4081 attgcacgaa acgaaatttt caggagaaaa gggtttaata gggcgtaata acgtcatgtt
     4141 taccctctct ttagcctact ttagttcagg ctattcaatc gaaacgtgcg aatataatat
     4201 gtttgagttt aataatcgat tagatcaacc cttagaagaa aaagaagtaa tcaaaattgt
     4261 tagaagtgcc tattcagaaa actatcaagg ggctaatagg gaatacatta ccattctttg
     4321 caaagcttgg gtatcaagtg atttaaccag taaagattta tttgtccgtc aagggtggtt
     4381 taaattcaag aaaaaaagaa gcgaacgtca acgtgttcat ttgtcagaat ggaaagaaga
     4441 tttaatggct tatattagcg aaaaaagcga tgtatacaag ccttatttag tgacgaccaa
     4501 aaaagagatt agagaagtgc taggcattcc tgaacggaca ttagataaat tgctgaaggt
     4561 actgaaggcg aatcaggaaa ttttctttaa gattaaacca ggaagaaatg gtggcattca
     4621 acttgctagt gttaaatcat tgttgctatc gatcattaaa gtaaaaaaag aagaaaaaga
     4681 aagctatata aaggcgctga caaattcttt tgacttagag catacattca ttcaagagac
     4741 tttaaacaag ctagcagaac gccctaaaac ggacacacaa ctcgatttgt ttagctatga
     4801 tacaggctga aaataaaacc cgcactatgc cattacattt atatctatga tacgtgtttg
     4861 ttttttcttt gctgtttagc gaatgattag cagaaatata cagagtaaga ttttaattaa
     4921 ttattagggg gagaaggaga gagtagcccg aaaactttta gttggcttgg actgaacgaa
     4981 gtgagggaaa ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac acttgatttt
     5041 ttaattttct atcttttata ggtcattaga gtatacctat ttgtcctaat atgattttag
     5101 cagtataatt gacttggtga ataggtcatt taagttgggc ataataggag gagtaaaatg
     5161 aaaaaattta tttatcgagt tttagaaaat gacgaagtgg tggctatttt taatgagcaa
     5221 caatatgcgc aagattttat cgcttacgaa aagacaattt ctgataagca atttgaaatt
     5281 gaaaaagtag atattgctga ttggttattg caaccgagag aattttagag gttggttgaa
     5341 aatggctaaa attggttatg cacgtgtcag tagcaaagaa cagaacttag atcggcaatt
     5401 acaagcgtta cagggcgttt ctaaggtctt ttcagacaaa ttaagcggtc aatcggtcga
     5461 acgcccacaa ttacaagcta tgcttaacta tattcgtgaa ggggatattg ttattgttac
     5521 tgaattagat cgattaggac gaaataataa agaattaaca gaattgatga atcaaattca
     5581 aattaagggg gcaaccctgg aagtcttaaa tttaccctca atgaatggta ttgaagatga
     5641 aaatttaagg cgtttgatta atagccttgt cattgaattg tacaagtatc aagcagaatc
     5701 agaacgaaaa aaaattaagg aacgtcaggc acaaggaatc gaaattgcta agaaaaaagg
     5761 caaattcaaa ggtcgtcagc ataaatttaa agaaaatgat ccacgtttaa agcatgcttt
     5821 cgatttgttt ttgaatggtt tatccgataa agaagttgaa gaacaaactg gaatcaatcg
     5881 ccgaacgttt agaaggtatc gagcaagata taacgtgaca gtcgatcaaa gaaaaaacaa
     5941 tgaaaagagg gatagttaat gagtacggtt attttagctg aaaaaccaag ccaggcatta
     6001 gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg gttattttga gatcaaagac
     6061 ccactattta cagatgaaac gtttatcacc tttggttttg ggcatttagt ggaattagca
     6121 gaaccaggtc attatgacga aaagtggcaa aattggaaac ttgaatcttt gccgattttt
     6181 cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa aattgttgca
     6241 gaacttctca aaaaggcaaa tacaattatt gttgcaacag atagcgacag agaaggtgaa
     6301 aatatcgcct ggtcgattat ccataaagca aatgcctttt caaaagataa aacatttaaa
     6361 agactatgga tcaatagctt agaaaaagat gtaatccgaa gcggttttca aaatttgcaa
     6421 cctggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg
     6481 ttgatcggca tgaacgcaag ccctttgtat acgttaaatt tacaacagaa gggcgtacaa
     6541 ggtacatttt cactaggacg tgttcaaacg cccaccttat accttatttt tcagcgccag
     6601 gaagccatag agaattttaa aaaagaacct tttttcgagg tggaagctag tataaaagta
     6661 aaccaagggt cgtttaaggg cgttctaagc cccacacagc gttttaaaac ccaagaggag
     6721 cttttagctt ttgtttcttc taaacaagct aaaataggca atcaagaggg gataattgct
     6781 gatgttcaaa ccaaagagaa gaaaacgaat agtccgagtt tgttttcttt aagtagtttg
     6841 caatcaaaag tcaatcagct ttataaagcg acagcgagcc aaactttaaa agctatttct
     6901 tttttaataa cttaaaaata aacttaatgt aacagcaagc acagtcaagg tatacacctt
     6961 tgacaaaaaa tagcacattc tctatcgaaa atttttgctt attttttaaa ttattttggg
     7021 aaattttccc aatccctttt tctaactcaa aa
//
LOCUS       DBKOIJ010000401         5851 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.158, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000401 DBKOIJ010000000
VERSION     DBKOIJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.158"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            322..1014
                     /locus_tag="ACV5NJ_003066"
     CDS             322..1014
                     /locus_tag="ACV5NJ_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HHY6487048.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="ACV5NJ_003067"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="ACV5NJ_003067"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HHY6487049.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="ACV5NJ_003068"
     CDS             complement(1910..3862)
                     /locus_tag="ACV5NJ_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HHY6487050.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4165)
                     /locus_tag="ACV5NJ_003069"
     CDS             complement(3869..4165)
                     /locus_tag="ACV5NJ_003069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6487051.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="ACV5NJ_003070"
     CDS             4507..4797
                     /locus_tag="ACV5NJ_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HHY6487052.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(5069..5418)
                     /locus_tag="ACV5NJ_003071"
                     /pseudo
     CDS             complement(5069..5418)
                     /locus_tag="ACV5NJ_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5511..5813
                     /locus_tag="ACV5NJ_003072"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="ACV5NJ_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1997 a          925 c         1010 g         1919 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcca t
//
LOCUS       DBKOIJ010000402         4034 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.203, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000402 DBKOIJ010000000
VERSION     DBKOIJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.203"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            <1..1149
                     /gene="vanS"
                     /locus_tag="ACV5NJ_003073"
     CDS             <1..1149
                     /gene="vanS"
                     /locus_tag="ACV5NJ_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HHY6487053.1"
                     /translation="IKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKLG
                     DWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFD
                     EINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYL
                     AHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNL
                     QTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNI
                     LKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDT
                     GGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            1364..2332
                     /locus_tag="ACV5NJ_003074"
     CDS             1364..2332
                     /locus_tag="ACV5NJ_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HHY6487054.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            2325..3356
                     /gene="vanA"
                     /locus_tag="ACV5NJ_003075"
     CDS             2325..3356
                     /gene="vanA"
                     /locus_tag="ACV5NJ_003075"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HHY6487055.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            3362..3970
                     /locus_tag="ACV5NJ_003076"
     CDS             3362..3970
                     /locus_tag="ACV5NJ_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HHY6487056.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1229 a          773 c          954 g         1078 t
ORIGIN      
        1 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
       61 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
      121 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
      181 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
      241 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
      301 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
      361 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
      421 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
      481 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
      541 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
      601 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
      661 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
      721 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
      781 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
      841 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
      901 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
      961 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     1021 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     1081 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     1141 aggtcctaag agatgtatat aattttttag gaaaatctca aggttatctt tactttttct
     1201 taggaaatta acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc
     1261 gtaagaacga gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc
     1321 ccgttttatt tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat
     1381 tactgtttat ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt
     1441 tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc
     1501 tttcaatcaa tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc
     1561 gctgaagaga gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga
     1621 tacaactgct gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag
     1681 cgttgccgat tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt
     1741 gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga
     1801 catgacagtt ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg
     1861 aggatttgga tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt
     1921 accgtttgat gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac
     1981 ggatacgcac tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct
     2041 tatcaatact gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa
     2101 cgggaaactg ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc
     2161 tgattgcacc caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa
     2221 cgtgataatc acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga
     2281 aaaaaccatt aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag
     2341 ttgcaatact gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag
     2401 agatagccgc taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat
     2461 ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt
     2521 cagctgtact ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat
     2581 atgaaatcaa ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg
     2641 gatccataca aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa
     2701 gctcagcaat ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag
     2761 ctactcccgc cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct
     2821 atcctgtttt tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata
     2881 gcgcggacga attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa
     2941 ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt
     3001 tagttgttgg cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg
     3061 aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag
     3121 cagaggagcg aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta
     3181 gaggtctagc ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag
     3241 tcaatactct gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag
     3301 gtattgcact tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag
     3361 catggaaata ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa
     3421 atatgccact tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat
     3481 tgtagggaca tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca
     3541 agggtacgga ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat
     3601 gcaatgggct gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga
     3661 ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag
     3721 tgccattgat cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg
     3781 atttgatttt atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc
     3841 gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct
     3901 cgaatggtgg cactatgtat taagagacga accatacccc aatagctatt ttgatttccc
     3961 cgttaaataa acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa
     4021 agaataaaat ccct
//
LOCUS       DBKOIJ010000403         3520 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.217, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000403 DBKOIJ010000000
VERSION     DBKOIJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.217"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            complement(1..998)
                     /locus_tag="ACV5NJ_003077"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="ACV5NJ_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="ACV5NJ_003078"
     CDS             1489..2427
                     /locus_tag="ACV5NJ_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HHY6487057.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="ACV5NJ_003079"
     CDS             2557..3204
                     /locus_tag="ACV5NJ_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HHY6487058.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT         1186 a          512 c          585 g         1237 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg
     3361 cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt
     3421 ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag
     3481 caaaagattt taaagagtta ttttatttta agtctttata
//
LOCUS       DBKOIJ010000404         3423 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.222, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000404 DBKOIJ010000000
VERSION     DBKOIJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.222"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            57..677
                     /locus_tag="ACV5NJ_003080"
     CDS             57..677
                     /locus_tag="ACV5NJ_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6487059.1"
                     /translation="MKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIERGLNY
                     PAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGHYDNFF
                     DSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDDLYSMD
                     MFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            831..908
                     /locus_tag="ACV5NJ_003081"
                     /pseudo
     CDS             831..908
                     /locus_tag="ACV5NJ_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            1222..1269
                     /locus_tag="ACV5NJ_003082"
                     /pseudo
     CDS             1222..1269
                     /locus_tag="ACV5NJ_003082"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1285..3204
                     /gene="tet(M)"
                     /locus_tag="ACV5NJ_003083"
     CDS             1285..3204
                     /gene="tet(M)"
                     /locus_tag="ACV5NJ_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HHY6487060.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            3305..>3423
                     /locus_tag="ACV5NJ_003084"
     CDS             3305..>3423
                     /locus_tag="ACV5NJ_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HHY6487061.1"
                     /translation="MNYKWILCPVCGNKTRLKIREDTELKKFPLYCPKCRQEN"
BASE COUNT         1206 a          501 c          708 g         1008 t
ORIGIN      
        1 ttaagcatga tagacttata atagcgaaga tataacgaga tatggagggt tatgcaatga
       61 aaataagcca tttaggaaat aatatacaga ccataagaaa atttagggga atgaaacaac
      121 aggaacttgc ggacaaaatc ggtatcaata tgcagagcct ttccaagatt gaaagagggt
      181 tgaactatcc tgcctatgaa acgctggaaa agataatgga agtgctggac gttacgccca
      241 atgaattgtt gtcgggagaa tggaagtatg tcaatcaatc tgaaaaggaa gtctgtcagt
      301 ttttaagagc cgaagaacgt ctaaatgcag aactgaaaca tggacactat gataacttct
      361 ttgacagcga ggaagaatgg ctggaatatg agttagaaaa gttacgggaa tatattacag
      421 actatatcaa cgggaaaagc attgaagcgt ccgaccttta cccaatcaaa gaatttatcc
      481 agcatttgaa gtttcaaaaa ttattagacc gctatgatga tttatacagt atggatatgt
      541 ttggggaaag catagaggga cataagtata agacacctta tcaagtcgtg aaaatgataa
      601 acccgaactc aaaagaagat atggaactgt tacgagaagt attaaggaat aacaattttg
      661 atgatgagga cgagtaactt ttttcctctt gcgagaagat acagcaaaca aaaagcccga
      721 tcaagagtgc aaagcaccac caatggtggc aaagctcttg acagggcttt ttcttttctg
      781 ttgtgggcaa tcaagaggaa gaaagggata aaatctgatt tgtggtaaat caggcaaagg
      841 caacgcaggt atctcaattt gatttggtac ttgaaaagaa cgggagtaat tggaagatta
      901 tagaataaca aatattggta cattattaca gctattttgt aatcacgtac tctctttgat
      961 aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa gtatctattt
     1021 aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagtgg
     1081 gaacaaataa ttggatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa
     1141 tacctttatc atgtgattct aaagtatccg gagaatatct gtatgctttg tatgcctatg
     1201 gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg
     1261 ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt
     1321 gatgcaggaa aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa
     1381 ttaggaagcg tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga
     1441 ggaattacaa ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc
     1501 atagacacgc caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta
     1561 gatggggcaa ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta
     1621 tttcatgcac ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa
     1681 aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt
     1741 gtaatcaaac agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct
     1801 gaacaatggg atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt
     1861 aaatcattag aagcattgga actcgaacaa gaggaaagca taagatttca gaattgttct
     1921 ctgttccctc tttatcatgg aagtgcaaaa agtaatatag ggattgataa ccttatagaa
     1981 gttattacta ataaatttta ttcatcaaca catcgaggtc cgtctgaact ttgcggaaat
     2041 gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg catatatacg cctttatagt
     2101 ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca
     2161 gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta ttctggagaa
     2221 attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga tacaaaacta
     2281 ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac tgttgaaccg
     2341 agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc agatagtgat
     2401 ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc tttcttaggg
     2461 aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa
     2521 ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt
     2581 cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt
     2641 ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatcg
     2701 tttcaaaatg cagttatgga ggggatacgc tatggctgtg aacaaggatt gtatggttgg
     2761 aatgtgacgg actgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc
     2821 ccagcagatt ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga
     2881 acagaattgt tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca
     2941 cgagcataca acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat
     3001 aatgaggtca ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat
     3061 ttaactttct ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt
     3121 actaccggtg aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga
     3181 tatatgttca ataaaataac ttagtgtatt ttatgttgtt atataaatat ggtttcttgt
     3241 taaataagat gaaatatttt ttaataaaga tttgaattaa agtgtaaagg aggagatagt
     3301 tattataaac tacaagtgga tattgtgtcc tgtatgtgga aataaaacac gattaaagat
     3361 aagggaagat actgaattaa aaaaattccc cctctattgt ccgaaatgca gacaagaaaa
     3421 ttt
//
LOCUS       DBKOIJ010000405         3278 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.227, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000405 DBKOIJ010000000
VERSION     DBKOIJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.227"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            3..188
                     /locus_tag="ACV5NJ_003085"
     CDS             3..188
                     /locus_tag="ACV5NJ_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HHY6487062.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACV5NJ_003086"
     CDS             203..1072
                     /locus_tag="ACV5NJ_003086"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HHY6487063.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACV5NJ_003087"
     CDS             1053..1787
                     /locus_tag="ACV5NJ_003087"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HHY6487064.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACV5NJ_003088"
     CDS             1820..2728
                     /locus_tag="ACV5NJ_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HHY6487065.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACV5NJ_003089"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACV5NJ_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DBKOIJ010000406         1127 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.313, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000406 DBKOIJ010000000
VERSION     DBKOIJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.313"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            411..959
                     /gene="aac(6')"
                     /locus_tag="ACV5NJ_003090"
     CDS             411..959
                     /gene="aac(6')"
                     /locus_tag="ACV5NJ_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HHY6487066.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          402 a          165 c          220 g          340 t
ORIGIN      
        1 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
       61 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
      121 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
      181 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
      241 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
      301 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
      361 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
      421 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
      481 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
      541 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
      601 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
      661 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
      721 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
      781 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
      841 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
      901 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
      961 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
     1021 acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg
     1081 tgtaccagag gttgtgaaaa agtcgcttag tttcgagtag taagaaa
//
LOCUS       DBKOIJ010000407          983 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.323, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000407 DBKOIJ010000000
VERSION     DBKOIJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.323"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            36..773
                     /gene="erm(B)"
                     /locus_tag="ACV5NJ_003091"
     CDS             36..773
                     /gene="erm(B)"
                     /locus_tag="ACV5NJ_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HHY6487067.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          357 a          172 c          167 g          287 t
ORIGIN      
        1 ttgtttatag atgtaatcac ttcaggagag attacatgaa caaaaatata aaatattctc
       61 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      121 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      181 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      241 aaaaattaaa actgaatact cgtgtcactt taattcacca agatattcta cagtttcaat
      301 tccctaacaa acagaggtat aaaattgttg ggaatattcc ttaccattta agcacacaaa
      361 ttattaaaaa agtggttttt gaaagccgtg cgtctgacat ctatctgatt gttgaagaag
      421 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      481 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      541 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      601 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      661 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc attacttatg
      721 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
      781 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
      841 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
      901 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
      961 aagtgtggca acaatattgt att
//
LOCUS       DBKOIJ010000408          810 bp    DNA     linear   BCT 10-NOV-2025
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4232
            SAMEA115707058-rid28051273.guided.340, whole genome shotgun
            sequence.
ACCESSION   DBKOIJ010000408 DBKOIJ010000000
VERSION     DBKOIJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707058
            Sequence Read Archive: ERR13196790
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-NOV-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002976926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002976926.1
            Genome Coverage       :: 168x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2025 23:25:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,934
            CDSs (with protein)               :: 2,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 40 of 99
            Pseudo Genes (incomplete)         :: 54 of 99
            Pseudo Genes (internal stop)      :: 21 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707058-rid28051273.guided.340"
                     /isolate="VRE4232"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
                     /collection_date="not provided"
     gene            92..787
                     /gene="vanR-A"
                     /locus_tag="ACV5NJ_003092"
     CDS             92..787
                     /gene="vanR-A"
                     /locus_tag="ACV5NJ_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HHY6487068.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
BASE COUNT          277 a          154 c          168 g          211 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag
//
